LOCUS       DAOQPK010000001        34434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_527_length_34434_cov_4.846069, whole genome shotgun sequence.
ACCESSION   DAOQPK010000001 DAOQPK010000000
VERSION     DAOQPK010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 34434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34434
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_527_length_34434_cov_4.846069"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PLU36_00005"
     CDS             complement(<1..403)
                     /locus_tag="PLU36_00005"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HMZ45161.1"
                     /translation="MNNSKIEKVCIVGLGYIGLPTAALLANNGYNVHGVDVKENVVNT
                     INKGEIHIVEPELDKYVKTAVNNGNLKASLQAQEADVFIIAVPTPFHEGFKPNIDFVL
                     DAAKKIAPYVKDGNIVILESTSPVSTTEKVAE"
     gene            complement(396..1532)
                     /gene="wecB"
                     /locus_tag="PLU36_00010"
     CDS             complement(396..1532)
                     /gene="wecB"
                     /locus_tag="PLU36_00010"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HMZ45162.1"
                     /translation="MKNILLVIGTRPEAIKIAPLIIKLQQQKALFNTKVCLTAQHRQM
                     LDQVLEFFEIKADYDLDLMKPNQNLHSLTSDIITAIKPVYDEFKPDWVLVHGDTTTST
                     AAALAAFYSGAKVAHVEAGLRTFNKLAPFPEEMNRSITGRIADLHFAPTKASKQNLLN
                     EAVSKETISITGNTVIDALKLGITKVNEQSSEIANLKSNINLNKKIILVTGHRRENFG
                     DGFENICKALKIIAENNFDDVEIIYPVHLNPNVQNPVNKFLSNQSNIKLIQPLNYEAF
                     IWLMNKSYLIITDSGGVQEEAPSLGKPVLVMREVTERPEAVDAGTVILVGTDTQKIIT
                     EAEQLLHNANAYTLMSNKHNPYGDGFACDRIIAILKEEKVTEYE"
     gene            complement(1560..2600)
                     /locus_tag="PLU36_00015"
     CDS             complement(1560..2600)
                     /locus_tag="PLU36_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45163.1"
                     /translation="MEIEEKEISIKDLLKSFKSWFWYILKQWWKIAGIAIIGAVIAIA
                     YRLWIPINYTAKSSFVVEEGRTSTGSLASLAGQFGFDIAGSSGATLFTGDNILIFFKS
                     NSLTKETLLTEYDSITKFSLADKYAEVYGLREKWAESKRIGKEIFFKTNQTTSLSRLQ
                     DSLLQTIVVKVLKKEFSVERPEKKATFIVVQCSFRDEMLSKLFCERIVKKATEWYVES
                     KTRRHRINVERLQKRADSIEQVLNGRTFYTAATQETTLDVNPAASTAAVKLEVSNRNK
                     LMVTQVYAEVIKNLEIGKVQLSQETPTILEVDNPDFPLPKTKRQFILYSLIGMIIFSF
                     FSIIYFTIKRLV"
     gene            complement(2622..5036)
                     /locus_tag="PLU36_00020"
     CDS             complement(2622..5036)
                     /locus_tag="PLU36_00020"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HMZ45164.1"
                     /translation="MKKVNKQILTKQLLFLIFFLSIVLASTAQLPANLSRVKSSDITD
                     SQLLEFIEKAKGSGLSEEQVIQELQRRRMSFAEIEAIKGRIAALVSPSKTNTSSAAIH
                     ESRTSVTTQKPDHSSKPTTIFGSELFSNPSLSFEPDLRLPTPKNYTLGPDDKLLLDVF
                     GVNLSQQNLTVGPEGTVYVKYAGPIQVNGLTIEDASKIITKKLSKFYPAISNGQTKVV
                     LSLTNIRAIKVMVLGAVRKPGTYNISSLATLFNALYVSGGPTENGSFRNIELVRNNKV
                     LLVADLYDFLINSNQKNNIRLMDNDIIKIPYAKVQVSIAGEVNREGIYELQPKENLNK
                     LIEYAGGYQSNAFKARITGLRNTDFDRQVLDIIKDSINTFIPQNGDSYNVGSIINKFQ
                     NRLIIEGAVFKPGNYALTTGLTVQELLHKAEGLREDAYTGRAVLVRTKDDLTKEIISI
                     NLLSDSGKNFILQKEDVLQVTSIFDIKEKFEVTINGAVRKPGAYSFEDSLSLKTLILR
                     AGGFAYNALGTDIVIARRKRNLDINNPKSSIVELIKINDDKDLSNPASDIQLKPFDII
                     TIKEDPYYKSQISVAISGQVLSPGSYTLTSRVEKISDLINRAGGTLYTANIDGARLLR
                     KNSVREDEFALIDKIAEASAHDSSGVTANSQNKLYKVIAIDLIKIMANPGDKEDIFLE
                     EGDIIEVPLKNNMVTIAGEVFNPIVVGYGENKKVRDYLNDAGGIAKSGNKRKVFIVYP
                     NGSAARTKKILGIFRKYPTVTPGSKIFVPREPEKKATDYAKAGIIVTGLSALITAVAL
                     AFQITK"
     gene            complement(5290..6582)
                     /locus_tag="PLU36_00025"
     CDS             complement(5290..6582)
                     /locus_tag="PLU36_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HMZ45165.1"
                     /translation="MKLEESKIAIIGLGYVGLPLAIEFGKKFSVLGFDINQNRISELV
                     EGKDRTNEADLQGLKQATLLYNSTNGTKGLFFSSDINLLKECTVFIVTVPTPINNFKA
                     PDLTPLIAASATISKVLKPNDIVIYESTVYPGCTEEDCVPVLEKYSGLKFNKDFYCGY
                     SPERINPGDKVNTLTKIKKVTSGSTPAIANFVDDLYASIITAGTHKAPSLKVAEASKA
                     IENAQRDVNISFVNELALIFDKMGIDTTDVIEAAATKWNFLKYKPGLVGGHCIGVDPY
                     YLAHKAESLGYHPQVILSGRRVNDNMGLFVASKVVKLMIAKGQKVNGAKALILGITFK
                     ENCPDIRNSKVIDIYNELKQYGMDVDVYDTHASKEEVKEEFGINLIDSLSKKYDALIL
                     AVSHNEFMHLNINQLIATDAVIFDTKAFLDRSIVDARL"
     gene            complement(6635..7114)
                     /gene="ispF"
                     /locus_tag="PLU36_00030"
     CDS             complement(6635..7114)
                     /gene="ispF"
                     /locus_tag="PLU36_00030"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HMZ45166.1"
                     /translation="MSFRIGFGIDFHQFAEGRDLWLGGIKIPHSKGAIGHSDADVLLH
                     AICDALLGALALGDIGLHFPNTEQAYKDIDSKILLKKSYELIKEKGYTIINIDSTVCL
                     ENPKIKPFVSSMQQTIAEILDITVNDISIKATTTETMGFAGREEGLVAYATVLLQKQ"
     gene            complement(7203..8375)
                     /gene="porV"
                     /locus_tag="PLU36_00035"
     CDS             complement(7203..8375)
                     /gene="porV"
                     /locus_tag="PLU36_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HMZ45167.1"
                     /translation="MRHSIWRVTALFLLTCSFSNVLKAQSTDINVVTTAVPFLRISPD
                     ARAGGMGDVGIATTPDANAPFWNLSKVPFTTKNTEISLTYTPWLKDLGLTDVYLASFA
                     GYHKLDEEQAVAASIRFFSLGNIQFTDYAGNPLQENRPREFSIDAGYTRKLNTKLSMG
                     VTLRYINSQLANGSIGGVNYKAGNAISGDISLYNYGLSEKGEGLSWGVVLSNLGTKIS
                     YTNDATNKDYIPANLGLGVAYTTVFDETSKLTFGLDVNKLMVPTPPTPVDPNEPNADA
                     RNAALLEEYRSMSVMKSWFKSFGDSGGFGNELKEFQVSLGAEYVYNDQFALRLGYFYE
                     DKSKGNRKFFTVGAGLKYNVIGINLSYLVPSGSGVTRNPLSNTLRFGLTFDIADKK"
     gene            8755..10200
                     /gene="gatB"
                     /locus_tag="PLU36_00040"
     CDS             8755..10200
                     /gene="gatB"
                     /locus_tag="PLU36_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HMZ45168.1"
                     /translation="MGEISKYEAVIGLEIHAQLQTNSKLFSGDSNHFEMEPNSNISPI
                     VLAHPGTLPKTNKKAVEYAIKMGLACNCSIEKNNYFARKNYFYADLPKGYQISQHTTP
                     ICKGGYVTIQMNENEEKKIQLNRIHLEEDAGKSIHDQHPNYSLIDFNRAGTPLIEIVT
                     EPCMNNSNEAYKYVNEIRKLVQWIDICNGNMEEGNLRCDANVSIRKKGEITLGTKVEV
                     KNLNSVRNIKKAIDFEIERMIALAEKGEKITQQTRSFDADTDTTFALREKEDTNDYRY
                     FADPDLAPFYISDNDINKLKNNLPVLPNLLKQKFIREFNLSLYDADQLISDKSTATYF
                     LNTTKFSTNHKAIANWINGPLKQHVNTKEDSFENLKLSPKKLALLIEIVENGTINFST
                     ASTKILPVLLAEDKQPMQIAEELNLVQINNTTEIEQWISIVLSNMPDKVEEYKKGKKG
                     LLGLFVGEVKKLSKGKADPKIATQLLTEKLQ"
     gene            10236..11339
                     /locus_tag="PLU36_00045"
     CDS             10236..11339
                     /locus_tag="PLU36_00045"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HMZ45169.1"
                     /translation="MKKLSLILLASTVIFSCKTKENGAFTVSGNINNASTDKVYLEEM
                     PFAAEKSIVIDSATIKDGKFELNGLANTEGLYALTILNGPRVLLVNDSKDIDVNLDVH
                     QYKNYQIKGSKATISLKNLFTDYEAQFSKVKRAYTILDSLKELVTSDSILTIHKLSKE
                     QEMGKLNKILSNFVTSTESPAACLQALVMATRSMPLEELQTLALNAASKFKDYDGMQK
                     FNTMLRGNQYPLLNQQAPEFTLNTPDNKPIALSSFKGKYVLVDFWASWCKPCREENPT
                     VVAAYNKYKDKNFTVFGVSLDSDKNSWVEAIKKDNLTWLHVSDLLQWETPMVGLYKFD
                     GIPFNVLIDPSGKIIATGLRGDALDKKLSEILN"
     gene            complement(11708..13285)
                     /locus_tag="PLU36_00050"
     CDS             complement(11708..13285)
                     /locus_tag="PLU36_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HMZ45170.1"
                     /translation="MEDRFLLLLILIMVMIGIVMLAKKIRVAYPILLVVAGLLISFIP
                     GMPTIELRPELIFILVLPPILYEAAWACSFRELFKWRRIILSFAFFGVFITAISVAFT
                     AYWFIPGFTLALGFLLGGIVSPPDAVSTNAITKFVKLPKRISTILEGESLLNDASSLI
                     IIQFAILAVVTGNFNASKASLQFAWMVIGGVACGLLFGWIFVKAHKKLPTDANMDVLL
                     TLIAPYLMYISAEEIHTSGVLSVVAGGLFVSQKRYDFLSSTSRVRGTNVWQSFVFLLN
                     GIVFILIGLDLPQITAGLEGTNITTAIGYGILITIVVILVRLLSFYGAVGVTMIMRNF
                     ITVADRQNPGFKGPFILGWAGMRGVVSLAAALSIPFYLDNGEVFPQRNLILFITFIVI
                     LLTLVIQGLTLPFLIKRAKLKEIDYPQPEDEVSAYLDKELLKISIDYLRSVFPEKEQK
                     SKRFHEILVNVQEQLSENNEIKLDSKSTHIYCELLTLQRKQLLQINKTQPTIDEEIIH
                     KKMMIIDYQEERLKLRN"
     gene            complement(13391..15040)
                     /locus_tag="PLU36_00055"
     CDS             complement(13391..15040)
                     /locus_tag="PLU36_00055"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HMZ45171.1"
                     /translation="MAKYIFVTGGVTSSLGKGIIAASLAKLLQARGFKVTIQKFDPYI
                     NVDPGTLNPYEHGECFVTEDGAETDLDLGHYERFLNIFTSQSNNVTTGRIYQTVINKE
                     RAGEFLGKTVQVVPHITDEIKRRMQLIGKTGEYDIVITELGGTVGDIESLPFIEAVRQ
                     LQWELPDEDVVVVHLTLVPYLKAAKELKTKPTQHSVKMLSETGVHPDVIVCRTEEPLG
                     NDIKRKIALFCNVKQEAVIEAMDASTIYEVPLLMLREKLDLFCLKKLNIKQFNDPQLD
                     KWKAFLDKLKYPKAKITIGLIGKYVELQDAYKSILESFIHAGAINEVKIQVVNIHSEF
                     ITKDNVAEKLEGLDGLLVAPGFGSRGIDGKIFAVQFARENSLPFFGICLGMQMAVIEY
                     GRNILGLNDAHSVEMEPTTVSPVINMMEEQKKIKMMGGTMRLGAYPCKLQEKSLAYKI
                     YGNSQISERHRHRYEFNNDYLEMYEQAGMIASGKNEETGLVEIIEIPSHPFFIGVQFH
                     PELKSTVEEPAPLFVHFVGAAKKYNETKSALISSLLQRETI"
     gene            15353..16009
                     /locus_tag="PLU36_00060"
     CDS             15353..16009
                     /locus_tag="PLU36_00060"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HMZ45172.1"
                     /translation="MTIHREGYQTIGITALFFGVFNVVSFYFFSASMPWLALLVFFIT
                     LFFLLFIISFFRIPNRKLTIDENKIICPADGKVVVIEETTDVEYFNDKRIQVSIFMSP
                     ANVHVNRNPMSGEVLYNKYHKGKYLVAWHPKSSTENERWSVVVKNNFGTILYKQIAGA
                     LAKRICNYTQVGQQVNQCKEYGFIKFGSRVDILLPIGTKINVQLNQKVQGGVTVLAEW
                     "
     gene            16172..16414
                     /locus_tag="PLU36_00065"
     CDS             16172..16414
                     /locus_tag="PLU36_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45173.1"
                     /translation="MKKFLMLAASVILFVGVALACGDKKDCKKGKDCCQKEAKAKNKE
                     ACSKESKDKVCCKKDGAEKKEVKKEEKKETKTEKKA"
     gene            complement(16484..17335)
                     /locus_tag="PLU36_00070"
     CDS             complement(16484..17335)
                     /locus_tag="PLU36_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMZ45174.1"
                     /translation="MKKTILTAVLLSAAYITFAQTESKENTEDTVKAGNFFIVKKNGK
                     TGNKESKGFKISIHNSEDKNKNISTNWFVLDLGFANYRDETNYTNAQAGSYLRTLYTP
                     AGAVTSQSLRLKTGKSSNVNLWFFMQRLNISQHVFNLKYGLGLEMYNFRFDRNVSYRK
                     DPNPFIFNDSINFSKNKLYAGYITLPLMVNINTKPNSKKSFSFSAGVSAGYLISTRNK
                     QVSDTRGKIKYKGDFDLQPFRLAAVGEVGVGPVRLYASYSLNALHNKATLLNQYPYAI
                     GLRLSSW"
     gene            complement(17367..18473)
                     /locus_tag="PLU36_00075"
     CDS             complement(17367..18473)
                     /locus_tag="PLU36_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45175.1"
                     /translation="MKMEINRNNYQEYFLLYLDDELSVHQKNMVDEFLLIHKDLKVEF
                     NLFKQTKLPNPTLISANKSTLYRFDIDTIHKANYEENFLLYADNELDENKRQAVEKFV
                     LQNPSYQNEFTVLKMATLQPENIQCPFKNELLKNERKPIIYFGFKPVAIAASLVLIAV
                     AVWLLNFNNTIINNNNIAFNSQTNNSQNNNTSSINNLQAKTSIPTISNNKVQPSTLYN
                     QQEEPKVQSTSNTNLATTIINQNNEVNNAVTNSTVSTIHNTTKQEQNIVEVDLKSVYQ
                     NANYKTLQTSNNTALNTLIQVNNNIEETNMVQQSTFQYIDINEITENESSNEKLINIG
                     NIKVKKSKLKNIFKKAKKIIGAPIDNKAIALATL"
     gene            complement(18497..18982)
                     /locus_tag="PLU36_00080"
     CDS             complement(18497..18982)
                     /locus_tag="PLU36_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HMZ45176.1"
                     /translation="MTERDYNNCVNQYADNVYRFILKNIKQEEDAKDIVQTAFEKLWR
                     NKENVETDKSKSYLFTIAYNQMIDHIRKNKRIVLTESFKDNVRINHPTDSFSKNVFDE
                     ALSKLNDIQKSLVMLKDYEGYSYEEIGKITNLNASQVKVYLHRARIILRDFLVKRENV
                     L"
     gene            complement(19072..20196)
                     /gene="mqnC"
                     /locus_tag="PLU36_00085"
     CDS             complement(19072..20196)
                     /gene="mqnC"
                     /locus_tag="PLU36_00085"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HMZ45177.1"
                     /translation="MQLSNLYNKALQFEFLSIEEGMFLFKHAPLTELMHIANEMKKHH
                     VPHGKVTWQIDRNLNTTNVCIANCKFCNFYRIPGHSEAYITDMPTYRKKIQETINYGG
                     DQLLLQGGHHPNLGLDFYTKIFREIKQEFPTIKLHALGPPEVAHITKLEKSTHYEVLK
                     ALQNAGLDSLPGAGAEILVDRVRRLVSKGKCGADEWLEIMHQAHKLNITTSATMMFGH
                     VETVEERFIHLSKIREIQAKKPDSAKGFLAFIPWTFQDVDTLLAKIRGVHNLTTSEEY
                     IRTIAISRIMLPNIKNIQASWLTVGKETAQICLHGGANDFGSIMLEENVVSAAGAPHR
                     FTYKTIQNAISEAGFGPQLRNQQYEWRDLPNFIQEQVINY"
     gene            20326..21585
                     /gene="chrA"
                     /locus_tag="PLU36_00090"
     CDS             20326..21585
                     /gene="chrA"
                     /locus_tag="PLU36_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HMZ45178.1"
                     /translation="MILLRHIPFLKAVFLYSITAFGGPQGHYGMMLKTFVKQRKYVSE
                     KELLDLVSFCNMIPGASSTQTITLIGYKRGGTGLALLTLLIWIFPASVLMGGLSFLLS
                     YLDKNSINTQWFRFIQPMAVGFIAFSSLKLYKVCINNTITRIILVAASFITFFAFKSP
                     WVFPVLIICAGIATNFSDKRIPQKTLPTQKIKWANILIFLFLFGLTGYISETATRQNW
                     ENRKAINLFENNYRFGSLVFGGGDVLMPMMYEQYVVRPKTEKILKSKRDVLKMSKEEF
                     LTGSGMVRAIPGPVFSIASFTGGMLLRNEGSPLLHVLGCVLGVIGIFLPSALLVLFFF
                     PIWHNLKKYAVIFRSLEGINAVVVGIMAGAFFYLAKDILFTDVQISLKNFSLDVVVII
                     ATFLTLEYTKTKAPFIVFACLLLGWLL"
     gene            complement(21587..22204)
                     /gene="recR"
                     /locus_tag="PLU36_00095"
     CDS             complement(21587..22204)
                     /gene="recR"
                     /locus_tag="PLU36_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HMZ45179.1"
                     /translation="MQLPSVLLENAVAEFAKLPGIGKKTALRLVLHLLNKNEEEVKLF
                     GDTIYTMRNSIKFCNRCFNISDGDICSICSNSMRNQQIICVVENIRDVIAIESTQQYN
                     GVYHVLGGVISPLDGIGPDKLNINGLLSRVENDQVEEIVFALSPNIQGDTTIYYIQKK
                     LQNTNCKITTIARGIAFGGELEYADEMTLAGSLQNRLPVQQYVNG"
     gene            complement(22238..23125)
                     /locus_tag="PLU36_00100"
     CDS             complement(22238..23125)
                     /locus_tag="PLU36_00100"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HMZ45180.1"
                     /translation="MAFNIQTFKTRTLSAIVFAAIMLFGLFVNNWTFFLLFTIIHFGC
                     WIEYQKLLSLIDDCYKTITPFHKYGVIITGFGFMLWMLNASYAFGSFNLQLLGMSLVV
                     IFAIVLPITEILFSKKQEVKPAFLSFLGLIYISMSIGLILDLYEININNAIIGNGIIA
                     IFIIVCMWVNDTMAYIVGSFIGKTPFSKISPKKTWEGTIGGIVLCIIVMGFAMNYFIE
                     EKQLIGQSASLKVHWFILAAIASITGTLGDLLESKLKRMAGVKDSGNIMPGHGGFLDR
                     FDSLLVAVPFIWLYVKLFL"
     gene            complement(23293..23640)
                     /gene="ssb"
                     /locus_tag="PLU36_00105"
     CDS             complement(23293..23640)
                     /gene="ssb"
                     /locus_tag="PLU36_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HMZ45181.1"
                     /translation="MYALKNKVQLIGNLGNNPEVKTLETGKKMARFSMATKEVFRNAK
                     GEKVVETQWHNIVAFGKVADIVEKYLQKGTEMAMEGKLINRSYIDKEGNKKYITEVQV
                     NEVLLLGKENAKA"
     gene            23940..24332
                     /locus_tag="PLU36_00110"
     CDS             23940..24332
                     /locus_tag="PLU36_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45182.1"
                     /translation="MEHSIVKKCLSCGKVVKGRTDKKFCDDYCRNNFNNQKKSKENYS
                     PYVKGILNTLLKNRKILETLLPPDKQTIHFKKEKLVQAGFIFKYFTHTYINKQRETYF
                     YCFDFGYKQLENDWLMIVKRREGDERSI"
     gene            complement(24469..24542)
                     /locus_tag="PLU36_00115"
     tRNA            complement(24469..24542)
                     /locus_tag="PLU36_00115"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24506..24508),aa:Asn,seq:gtt)
     gene            complement(24644..24994)
                     /locus_tag="PLU36_00120"
     CDS             complement(24644..24994)
                     /locus_tag="PLU36_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HMZ45183.1"
                     /translation="MQDIKWYKIADSINLLPWQANNMCLVEVKNKIYTIAKHNNKIFA
                     FSHKCPHASGILANGWIDALANVVCPIHRYKFCMQNGRNTSGEGYFLKTYKVEIREEG
                     VFIAIENKGFLSFF"
     gene            complement(25001..26188)
                     /gene="hflX"
                     /locus_tag="PLU36_00125"
     CDS             complement(25001..26188)
                     /gene="hflX"
                     /locus_tag="PLU36_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HMZ45184.1"
                     /translation="MIEKQQKTNKQERVILVGVIQKEQTIEQVNEYMDELAFLAETAG
                     ALTVKRFTQKLQHPDSKTFVGKGKLEEIKNYIEGRNIDMVIFDDELTGSQLVNIEKVL
                     GIPIIDRSDLILDIFASRARTAQAKVQVELAQYQYILPRLKGMWKHLERQGGGIGTRG
                     PGETEIETDRRIVKDKISLLRKKLGEIDKQSFTQRKERGELIRVSLVGYTNVGKSTIM
                     NLLSKSDVFAENKLFATLDTTTRKVVFEATPFLLSDTVGFIRKLPHHLIESFKSTLDE
                     VREADILLHVVDIAHPQYEDQIGTVNKTLQDLKAFDKPVLTIFNKMDLYEKRYFDEWL
                     HSEVKQDILLELKEKWVNNTDNNCVFISALERKNIDELRAVILNKVRDIYQIRYPYKT
                     IHY"
     gene            complement(26296..26475)
                     /locus_tag="PLU36_00130"
     CDS             complement(26296..26475)
                     /locus_tag="PLU36_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45185.1"
                     /translation="MLLLETKRIIDDEGLFRTLKIGFNILRFPKARKHILVMKEVFNK
                     YKDQMNAISIIAQKA"
     gene            complement(26563..27072)
                     /locus_tag="PLU36_00135"
     CDS             complement(26563..27072)
                     /locus_tag="PLU36_00135"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ45186.1"
                     /translation="MSNKIINTEQGHWLLAKMGKKVLRPGGKELTLKLIKGLKISNSD
                     NVIEFAPGIGYTAMKVLKFNPKSYTGVELNEEAATLLKRKINSNNRKIIIGNASNVML
                     EDASADKVYAEAMLTMQADHRKSEIIKEAYRLLKKGGLYGIHELGLTPDNLNEDTKAK
                     IQHELATCI"
     gene            complement(27432..28121)
                     /locus_tag="PLU36_00140"
     CDS             complement(27432..28121)
                     /locus_tag="PLU36_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45187.1"
                     /translation="MAIINIANNMGYKNAMFLLSILISFVLYGQKKIIFKDNFVNNKN
                     RWKLEKADDEFSVRIKDKALYFKKNHRNAVNNGCLWFEKNIRKFKAEKNFIITFYAKI
                     LSSDDVSNGFDFQWGKIEEDTNTSSSISTSLYQLDFGLTDMRLSKFDNINGWTYYDWS
                     DSYTETLNYTLQYGILYKYEIIQRNDTLTVLINDKIVYALPIKPQDGKSIGVQQCLKS
                     HWLLSRLSIKQ"
     gene            28204..28332
                     /locus_tag="PLU36_00145"
     CDS             28204..28332
                     /locus_tag="PLU36_00145"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HMZ45188.1"
                     /translation="MVIIAFYNLKGGVGKTASAVTFAYLAAANGLKTLVWDLDPQV"
     gene            28534..28782
                     /locus_tag="PLU36_00150"
     CDS             28534..28782
                     /locus_tag="PLU36_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45189.1"
                     /translation="MGTTAEKNTTLIDGYVGMLVNLSTTNKLDLISKLTASVKTDLTN
                     KKSSFKKAYGAFESKKSAEEIIEEIRNSRVSTRQIEAF"
     gene            28779..29180
                     /locus_tag="PLU36_00155"
     CDS             28779..29180
                     /locus_tag="PLU36_00155"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HMZ45190.1"
                     /translation="MKKYLIDTNIAVFYLRGKHNLESKFDQVTADDCYISEITLAELK
                     FGVEKSEKPEKNRKVLENFLTGVQVLPIFHSLDLYAKEKARLQKAGTPIDDFDLLIGV
                     TSITHNLTMVTNNTNHFKNIREIDLEDWTQA"
     gene            complement(29508..31661)
                     /locus_tag="PLU36_00160"
     CDS             complement(29508..31661)
                     /locus_tag="PLU36_00160"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HMZ45191.1"
                     /translation="MKKVININFQGRVIPIEENAYDILKRYIESLGKLFANEEGKEEI
                     INDIEGRIAELFGEVLKKGSTCITAEDVNTIINSMGRPEDFDEDISQPNADKQQHQNY
                     EHQNSYSNTSDTKKMYRDENNKVLGGVCSGIANYFGIDPVVTRVIFVIFFGVMFVPYI
                     ILWVAIPSSSTQVIGSRRKRLFRDPDDKILAGVCSGISHYFGISVWIPRLLFLIPFIS
                     FIFRFNTWDIWDFPNFLKISFSPGALFIYIILWMILPEAKSSADKLEMKGEKVDLNNI
                     KNTIQSDIENFAKKAEKFGETISEKGAEFGNTVQEKGKQFSTEANTIFKKRGKGLGDI
                     IVLIVKIFAYFILGSVLLAIVVSLFGLGIVASGFLPLKGYIISTGWQNVFAWGTLLLF
                     IWVPIIAIVVFIVRRLAKIKGKSCLVRYTFSALWVLGLVCFINLLVSLSKDFKYRNNA
                     MEEQVLLTNPTVNKLEINPNNLTNYYNNNWFDMAPFADVDEDSSVIKNVRIRITKATN
                     DSFSVKMVKLSNGNSKKQANELAQKINFSIEQKDTLLYLGRGITINPKDKFRNQNVII
                     SIAVPIGKKIIIKRNAGWSWNRNIHVSFFDDSDFDWYNLDGSELAWKHNVEYIMTNEG
                     LKRTNNKYDEDDDDDFRMNEKDYLQKEYEEAQQKADRLKQKLDNNTKDSTGLDEKKYR
                     YEPTTPKTSNTPTKSTVQIPNNNAISKNILIKFGI"
     gene            complement(31694..32032)
                     /locus_tag="PLU36_00165"
     CDS             complement(31694..32032)
                     /locus_tag="PLU36_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HMZ45192.1"
                     /translation="MDIQNTQSQMRKGVLEFCILSIIRQGEVYPSDIADKMKAANLQI
                     LEGTLYPLLTRLKNAGYLTYRWVESNSGPPRKYFVMTDKGLDFYNELGKTWQELADAV
                     ATLTQQNNNS"
     gene            complement(32090..32284)
                     /locus_tag="PLU36_00170"
     CDS             complement(32090..32284)
                     /locus_tag="PLU36_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45193.1"
                     /translation="MKTQQTNLLQPLTNNEIVKLTTTQNEIVAVADNNNNKVFSSADL
                     WNLRRSRKSVTPIRRAAFIW"
     gene            32680..33600
                     /locus_tag="PLU36_00175"
     CDS             32680..33600
                     /locus_tag="PLU36_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HMZ45194.1"
                     /translation="MSLVVVGSMAFDAIETPFGKSDKIIGGAGTYIAFCSTYFTPVKQ
                     ISVVGGDFPEDELDELSKRNVCLEGVQIKKDEKTFFWSGRYHMDMNSRDTLVTELNVL
                     GDFKPIVPESYQDCEFLMLGNLAPAVQLSVIEQLKNKPKLIALDTMNFWMEVAMDDLK
                     KVLEKIDVLIINDSEARQLSGDYSIVRAAKKIMTMGPKYLVIKKGEHGALLFHENNVF
                     VAPALPLEDVFDPTGAGDTFAGGFIGHLAKTKDISFENMKTAIIVGSAMASFCVEKFG
                     TERLRNLSKDAIAERINEFVQLVHFDVSLV"
     gene            complement(33655..>34434)
                     /locus_tag="PLU36_00180"
     CDS             complement(33655..>34434)
                     /locus_tag="PLU36_00180"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HMZ45195.1"
                     /translation="HKPFTTEHKQQATASNLFKTFTGKHQAHKIEPNEKSELKHWKDF
                     FTTRPIVEINNEPEVEQKIQYNQLQNVESLFIQLHKTYILTTNQAGFLLVHQQHAHER
                     VLYEKYSAYSHNKQIATQKNLFPITFELATPDAILLEDLMPEIITIGYQIEPFGNNTF
                     IIQGTPADVGTGNEKHSLELLLEQFKHFSSDIKYSKREKLVRCISRQQAIKAGTTLSI
                     TEMQNLLESLFQCTTPNVTPTGNPTYIEFKEDYLDRMFGKM"
BASE COUNT        11380 a         5510 c         4883 g        12661 t
ORIGIN      
        1 cttctgctac tttttcagtt gtgcttacag gtgaagttga ttctaaaata acaatattac
       61 catctttaac ataaggggca atttttttag ctgcatctaa cacaaaatca atattgggtt
      121 taaacccttc atgaaatggt gttggtactg caattataaa tacatcggct tcctgtgctt
      181 gtaaacttgc ttttaaatta ccattattaa cagcagtttt tacatactta tctaactctg
      241 gttctacaat atgtatttct cctttattta ttgtgtttac cacattttct tttacatcta
      301 caccatgtac attatagcca ttattggcta atagtgctgc tgttggcagg ccaatatatc
      361 ctaaaccaac aatacatact ttttctattt tactattatt catattctgt tactttctct
      421 tcttttaaaa tggcaataat tctatcacat gcaaagccat cgccataggg attatgctta
      481 ttactcatta aagtataagc attggcattg tgtaataatt gttctgcttc agtaataatt
      541 ttttgggtgt cagtaccaac taaaattaca gttcctgcat ctacagcttc tggtcgttct
      601 gttacttctc tcattactaa aacaggctta cctaatgatg gagcttcttc ctgcacacct
      661 ccactatctg taattataag gtagctttta ttcattaacc aaataaatgc ttcataattt
      721 aatggttgaa ttaatttaat attggattga ttagagagaa atttatttac cggattttga
      781 acatttggat ttaagtgaac cggataaata atttcaacat catcaaaatt attttcagca
      841 ataattttta atgctttaca aatgttttca aatccatctc caaaattttc tcttctatgc
      901 ccagttacta aaattatttt tttatttaga ttaatatttg attttagatt tgctatttcg
      961 ctgctttgct cattaacttt tgtaatacct aatttaagtg catcaataac tgtattacct
     1021 gtaatagaaa tagtttcttt agaaactgcc tcgtttaata aattttgttt agaagctttt
     1081 gtgggggcaa aatgcaaatc tgcaattctg cctgtaatgc ttctattcat ttcttctggg
     1141 aaaggtgcta gtttattaaa tgttcttaat ccagcttcaa catgagccac ttttgcccca
     1201 gaataaaaag ctgctaatgc agcagcagta gatgttgtag tatcgccatg tactaaaacc
     1261 caatctggtt taaattcatc gtacacaggt tttattgcag taataatatc ggaagttaag
     1321 gaatgtagat tttgatttgg tttcattaaa tctaaatcat aatctgcttt tatttcaaaa
     1381 aattcaagta cttgatccaa catttgccta tgttgtgcag ttaagcaaac tttagtatta
     1441 aataatgctt tttgttgttg aagtttaata attaatgggg ctatttttat tgcttccggc
     1501 cttgtaccaa ttaccaataa tatgtttttc ataaacctgt tatactataa ataaaataat
     1561 taaaccaatc tttttatggt gaaataaata attgaaaaga atgaaaatat aatcatgcca
     1621 attaaagagt atagaataaa ctgcctctta gttttaggta aaggaaaatc tggattatcc
     1681 acttctaaaa tagttggagt ttcttgactt agttgcactt taccaatttc taagttttta
     1741 ataacctctg catatacttg tgttaccatt aatttatttc tattactaac ctccagttta
     1801 acagctgctg tagatgcagc agggtttaca tctaaagttg tttcttgagt agcggctgta
     1861 taaaaagttc ttccatttaa aacctgctca atactatcgg ctcttttttg taatctttca
     1921 acatttattc tatgtcttct tgttttagat tctacatacc actctgtagc tttttttact
     1981 attctttcac aaaatagttt acttaacatt tcatctctaa agctacattg cactacaata
     2041 aaagttgctt ttttctctgg cctctctacc gaaaattctt tctttaaaac ctttactaca
     2101 attgtttgta acaaactatc ttgtagtctt gataggcttg ttgtttggtt ggttttaaaa
     2161 aatatttctt tacctattct tttactttct gcccactttt ccctaagccc atacacttcg
     2221 gcatatttat ctgctaatga aaatttggtt atagagtcat actcggtaag taaagtttct
     2281 tttgttaaac tattgctttt aaaaaatatt aaaatattat ctcctgtaaa taaagtagca
     2341 ccagacgaac ctgcaatatc aaatccaaat tgcccagcta aagaagccag acttcctgtt
     2401 gaagttctac cttcttctac cacaaaggaa ctttttgcag tataatttat gggtatccat
     2461 aatctatatg caatagcaat tacagcacca ataattgcaa taccagcaat tttccaccat
     2521 tgttttagta tgtaccaaaa ccaggattta aaagatttta aaagatcttt tattgaaatt
     2581 tctttttctt ctatttccat tagttgcaaa tattaaaatg tttattttgt aatttgaaaa
     2641 gctaatgcta cagcagtaat taatgcagat aatcctgtaa ctattattcc agcttttgca
     2701 tagtcggttg ctttcttctc tggctctctt ggaacaaata ttttacttcc tggtgttact
     2761 gtaggatatt ttctaaaaat gcccaatatt ttttttgttc ttgctgcact accattaggg
     2821 taaacaataa atacttttct tttattacca gattttgcaa tacctcctgc atcgtttaaa
     2881 taatccctta cttttttatt ttctccatac ccaactacaa ttgggttaaa cacttcgcct
     2941 gcaatggtta ccatattatt ttttaatggt acttcaataa tatctccttc ttctaaaaaa
     3001 atatcttctt tatcaccagg gtttgccatt atctttatta aatcaattgc aattacttta
     3061 tatagtttgt tttgagagtt tgcagtaaca ccagatgagt cgtgtgctga tgcttctgca
     3121 attttatcaa ttaaagcaaa ctcatcttct cttacactat tttttcttaa tagtcttgct
     3181 ccatcaatgt ttgcagtata taaagtaccg cctgccctat ttattaaatc gctaattttt
     3241 tctactctgc ttgttaatgt ataagaacca gggcttaata cttggccaga aatggcaaca
     3301 gaaatttgcg atttataata aggatcttct ttgatagtga taatatcaaa tggttttaat
     3361 tgtatatcac tagcagggtt ggataaatct ttatcgtcat taattttaat taattcaaca
     3421 atagagcttt tggggttatt aatgtctaaa tttcttttcc ttctagcaat tacaatatca
     3481 gtgccaaggg cattataagc aaaaccacct gctcttaaaa ttaaggtttt taaggatagg
     3541 ctatcttcaa aagaatatgc tccaggctta cgaacagctc cattaatagt aacttcaaac
     3601 ttttctttaa tatcaaaaat agaagttact tgtaatacat cttctttttg taaaataaaa
     3661 tttttaccag aatctgataa taaattgatg cttataattt ctttggttaa atcatctttt
     3721 gttcttacta atactgctct tcctgtatag gcatcttccc ttaacccttc tgccttatgc
     3781 aataattctt gtacagttaa acctgttgtt agtgcataat ttccaggctt aaaaacagct
     3841 ccttctatta ttaacctgtt ttgaaactta ttgattatac ttccaacatt atacgaatcg
     3901 ccattttgag gaatgaaggt attgatagag tcttttataa tatctaacac ttgcctatca
     3961 aaatctgtgt ttcttaaccc ggttattctt gctttaaaag cgttgctttg atagcctcct
     4021 gcatattcta ttaacttgtt taaattttct ttaggttgta gttcataaat tccttctcta
     4081 tttacctcgc cagcaatact tacttgcact tttgcatagg gaattttaat aatatcgtta
     4141 tccattaacc tgatattatt tttttgattg ctgttgatta aaaaatcgta taaatcagcc
     4201 actaataata ctttgttatt tcttaccaat tcaatatttc taaatgagcc gttttcagtt
     4261 gggccaccac taacgtataa agcattaaat aaagttgcta atgaactaat attataggtt
     4321 ccaggttttc ttacagcacc caaaaccata actttaattg ccctaatatt ggttaaggac
     4381 aatactactt ttgtttgtcc attagatatt gcaggataaa atttagataa ttttttagtg
     4441 attattttag acgcatcttc aatggttaag ccattaacct gaattggtcc tgcatatttt
     4501 acatatactg ttccttctgg tcctacagtt aaattttgtt gagaaaggtt tacgccaaat
     4561 acatctaata ataatttatc atcggggcct aatgtatagt ttttgggagt aggcaatctt
     4621 aaatctggtt caaaacttaa acttggatta gagaaaagtt cggagccgaa aattgtagtt
     4681 ggctttgaac tatggtctgg cttttgagta gttacacttg ttctgctttc atgtattgca
     4741 gcactggatg tattagtttt acttggagag acaagtgctg caattcttcc tttaatggct
     4801 tctatttcag caaaactcat tcttcttctt tgcagttctt gtattacttg ttcttctgat
     4861 aaaccagagc cttttgcctt ttcaataaat tcaagcagtt gactatcggt aatatcagaa
     4921 gattttactc tgcttaaatt ggcaggcaac tgtgcagtag atgccaatac tatacttaaa
     4981 aaaaatatta aaaagagtaa ctgctttgta agaatttgct tatttacttt tttcatgagt
     5041 ttgatatttt agatattaac caataccttg gttagcattt gaattatagt aagaaataaa
     5101 aaattataca gctccgccac cttactcaca aattaggtga gtagccttta ttaagaaata
     5161 agattttagt caaaatttct tttattaaaa aaataattta aaggtggcaa atataagtct
     5221 aagcctattt taaattatta taattttatt ttataaaaag tattgtataa aattccaact
     5281 cctctaaaat tataaccttg catctacaat acttctatct aaaaatgctt tggtatcaaa
     5341 aattacagca tctgttgcta ttagttgatt aatgtttaaa tgcataaact cattatgtga
     5401 tacagctaat atcaatgcat cgtatttttt agataagcta tctattaaat taattccaaa
     5461 ctcttccttt acttcttctt tgctggcatg ggtatcatat acatctacat ccataccata
     5521 ttgtttcaat tcattataaa tatctatcac tttagagttt ctaatatcgg ggcaattttc
     5581 tttaaatgtt ataccaagta ttaatgcttt tgcaccattt actttttggc ctttagcaat
     5641 cattaatttt acaaccttac tggcaacaaa taatcccata ttatcattta ctcttctacc
     5701 acttaaaatt acttgtggat gatagcctaa gctttcggct ttgtgtgcta aatagtaggg
     5761 gtcaacacca atacagtgcc ctccaactaa acctggtttg tattttaaga aattccattt
     5821 tgtggcagca gcctcaataa catcggttgt atcaataccc attttatcaa aaattaaagc
     5881 cagctcattt acaaaagaaa tatttacatc tctttgtgcg ttttcaatgg ctttcgatgc
     5941 ttctgcaact tttaaacttg gggctttgtg tgtgcctgcg gttataatgc ttgcatataa
     6001 atcatctaca aaattggcaa tagcaggtgt ggagccagag gttacttttt taattttggt
     6061 taaagtattt actttatctc ctgggtttat tctttctggc gaataaccac aataaaaatc
     6121 tttattaaac tttaatcctg aatatttttc taaaactggt acacaatctt cttctgtaca
     6181 tcctggataa actgtagatt cgtaaattac aatatcatta ggctttaaaa ccttactgat
     6241 tgtggcagat gctgcaatta aaggcgttaa atctggagct ttgaaattat taattggggt
     6301 tggtacagtt acaataaaaa cagtacattc ttttaataag tttatatcag aagaaaaaaa
     6361 taaaccttta gtaccatttg ttgaattata caataatgtg gcttgtttca atccttgtaa
     6421 atctgcttca ttggttctgt cttttccttc tacaagctca ctaattctat tttgatttat
     6481 atcaaaccct aatacagaaa attttttacc aaattctatt gccaatggta atcctacata
     6541 acctaagcca ataatggcaa ttttactttc ttctaacttc atatatttat tattctaaaa
     6601 cttattatta aattttttgt ggtaaaatta tttattattg tttttgcaac aaaactgttg
     6661 cataagccac caatccttct tctcttcctg caaagcccat tgtttcggtt gtggttgctt
     6721 taattgaaat atcgttaact gtaatatcca aaatttctgc aatggtttgt tgcatactac
     6781 ttacaaaagg tttaattttt ggattttcta agcaaacagt actgtcaata tttataatgg
     6841 tatatccttt ttcttttatt aattcgtacg atttttttaa taaaatttta ctatctatat
     6901 ctttataagc ttgctctgta tttggaaaat gcaaaccaat atcgcctaat gccaaagccc
     6961 ctaataaggc atcgcaaatg gcatgtaata aaacatctgc atcgctatgg cctattgcac
     7021 cttttgagtg gggtattttg atacctccaa gccataaatc tctaccctcg gcaaattgat
     7081 gaaaatctat tccaaatcct attctaaaac tcatttaaaa aaatgtttac ttataaaata
     7141 ccaaaagaag gcatttcaaa aacaccttct ataaatttat ctcaagaaat agttagtata
     7201 aattatttct tatctgctat atcaaacgtt agtccaaagc gaagtgtatt tgaaaggggg
     7261 ttacgggtta caccacttcc tgatggaact aaataagata aattaatacc aattacatta
     7321 tattttaagc ctgcacctac tgtaaaaaac tttcggttgc ctttgctttt atcttcataa
     7381 aaataaccta gccttaatgc aaactgatcg ttgtaaacat attcggcccc taatgaaact
     7441 tgaaattctt ttaattcatt accaaaaccg ccagagtcgc caaagctttt aaaccaactt
     7501 ttcatcacac tcatacttct gtattcttct agtaaagcag catttcttgc atctgcatta
     7561 ggctcattgg gatctacagg ggttggtggc gttggcacca ttagtttgtt aacatctaat
     7621 ccaaaagtta gtttacttgt ttcgtcaaaa acagttgtgt aagcaacacc taaacctaaa
     7681 ttggcaggta tataatcttt atttgtagca tcgttagtat agctaatttt tgtacctaag
     7741 ttagataaaa caacacccca acttaaacct tctccttttt cactcaatcc atagttatat
     7801 aaagaaatat caccagaaat tgcattacct gctttataat ttactccacc tatagaccca
     7861 tttgctaatt gagagttgat atagcgtaat gtaacaccca ttgaaagctt tgtatttagt
     7921 ttacgtgtat aaccagcatc aatagaaaac tctctggggc ggttttcttg taatgggttt
     7981 cctgcataat cggtaaactg aatattgcct aagctaaaaa aacgaataga agctgcaaca
     8041 gcttgctctt catctaactt atgatagcct gcaaaactgg ctaaatacac atccgttaag
     8101 ccaaggtctt taagccaagg cgtataagtt aaactgattt cggtattttt agttgtaaaa
     8161 ggcactttcg ataaattcca aaaaggtgca tttgcatccg gtgtagtagc tattcctaca
     8221 tcacccatac ctcctgcacg tgcatctggc gaaattctta aaaatggtac cgcagttgta
     8281 acaacattaa tatctgtact ttgggctttt aatacatttg aaaatgaaca agttaataaa
     8341 aataatgctg taaccctcca aattgaatgt ctcatctaaa tcaaaaaaat ttaatatttg
     8401 taaaaataaa agaatatttc aaccagccaa atcttgctat ctgaaatatt gattaagtaa
     8461 aaattagccc attaaataaa tttggttaca aaaaacttta gtaattaata aaaatacaac
     8521 acttaaaaag cattatttgt ttgcaataat acaaaaatca gacacttaaa cagtattttt
     8581 cgctgtttat tgtacaattt tttattaatt ctttacttaa ataataattt tctggttcaa
     8641 ttaactactt atagccaata taaataatat aaacacttgt taaacattgt aatagaagct
     8701 aataaatttc tatttatata actgtgtaca atacctttac agccatcttt atttatgggt
     8761 gaaatatcaa aatacgaggc tgttattgga ttagaaattc atgctcaatt gcaaaccaat
     8821 agtaaattgt ttagtggcga tagtaaccat tttgaaatgg aacctaattc aaatataagc
     8881 ccaatagttt tagcacatcc tggcacttta ccaaaaacta acaaaaaagc agtagagtat
     8941 gccattaaaa tgggattagc atgtaattgt agcattgaaa aaaacaacta ttttgctcgt
     9001 aaaaattatt tttatgccga tttgcctaag ggctaccaaa tttctcaaca taccacacca
     9061 atatgtaagg gtggttatgt taccattcag atgaatgaaa atgaagaaaa gaaaattcaa
     9121 cttaatagaa ttcatttaga agaagatgct ggcaaaagca tacacgacca acaccccaac
     9181 tacagtttaa ttgattttaa ccgtgcaggc accccattaa tagaaattgt tacagagcct
     9241 tgcatgaaca atagtaatga agcatataaa tatgtaaatg aaataaggaa gttggtacaa
     9301 tggattgata tttgtaatgg gaatatggaa gaaggcaact tgcgttgcga tgccaatgtc
     9361 tccatcagaa aaaaaggaga aataacatta ggtactaaag tggaagtaaa aaatttaaac
     9421 tctgtaagaa atataaaaaa agcaattgac tttgaaattg aaagaatgat tgcactagca
     9481 gaaaaaggag aaaaaattac acagcaaaca agaagttttg acgctgatac agataccaca
     9541 tttgctttga gagaaaagga agacactaac gattatcgtt attttgccga cccagactta
     9601 gctccttttt acatttctga taacgatatt aataaactta aaaataactt acctgtttta
     9661 ccaaacttgc ttaaacaaaa gtttatacgt gaatttaatt taagtttata cgatgctgat
     9721 caactaatct cagataaaag cacagcaacc tattttttaa acacaacaaa attttctact
     9781 aaccataaag caattgcaaa ctggataaat gggccactaa aacagcatgt taacaccaag
     9841 gaagacagtt ttgaaaatct taaattaagc cctaaaaaac ttgctttatt aatagagatt
     9901 gtagaaaatg gtacaataaa tttttccact gcatctacta aaatattacc agttttacta
     9961 gccgaagaca agcaaccaat gcagattgca gaagaattaa atttagtgca aataaataat
    10021 accacagaaa ttgagcaatg gataagcatt gtactttcca atatgcccga taaagttgaa
    10081 gaatataaaa aaggtaaaaa agggctttta ggattatttg ttggagaagt aaaaaagctg
    10141 agcaagggta aagcagaccc taaaattgca acacaattat taactgaaaa actacaataa
    10201 aatagttata aattttaacc caaataagta gattaatgaa aaaactaagt ttaattttat
    10261 tagcctccac agtaattttt tcttgcaaaa caaaagaaaa tggagctttc acagtttcgg
    10321 gaaatattaa taatgccagt acagataaag tgtatttaga agaaatgccc tttgctgctg
    10381 aaaaatcaat tgtaatagac tctgccacta ttaaagatgg taaatttgag cttaatgggt
    10441 tagcaaatac cgaaggatta tatgctttaa caattttaaa cgggccaagg gttttacttg
    10501 taaatgacag taaagatatt gatgtaaact tagacgttca tcagtataaa aattatcaaa
    10561 taaaaggctc taaagctacc atttctttaa aaaatttatt tactgattac gaagcacaat
    10621 tttctaaagt aaaaagagca tacactattt tagattcgtt aaaagaatta gttacttctg
    10681 atagtatatt aactatacat aagttatcaa aagagcaaga gatgggtaag ttaaataaaa
    10741 ttttatccaa ttttgttaca tcaaccgaaa gccccgctgc ttgcttacaa gctttggtaa
    10801 tggcaacccg tagtatgcct ttagaagaat tgcaaacact tgccttaaat gcagcttcta
    10861 aatttaaaga ttatgatggt atgcaaaaat ttaataccat gttaagaggt aaccaatatc
    10921 ctttacttaa ccaacaagca cctgaattta ctttaaatac accagataat aagccaattg
    10981 ctttaagtag ttttaaagga aaatatgtat tggttgactt ttgggcaagt tggtgtaaac
    11041 catgtagaga agaaaaccca acagttgtag cagcctataa taaatacaaa gacaaaaact
    11101 ttacagtatt tggtgtatcg ttagacagcg ataaaaacag ttgggtagaa gcaataaaaa
    11161 aagataatct aacatggtta catgtaagtg atttactgca atgggagaca ccaatggtag
    11221 gcttatataa atttgatgga ataccattta atgtattaat agacccttca ggaaaaatta
    11281 ttgctaccgg attaagagga gatgctttag ataaaaaatt aagtgaaatt ttaaactagt
    11341 aataaaactt tcactaacaa tatttaaact tttgctagtg ctatctcaga gttttcttac
    11401 atattttcag agtaagaaaa ttctgagatt tttttactat tttattgagg ttaaaaaaat
    11461 aatctaggta acattcttca aacatgattt tgattaatcc tattttacaa taaagttaaa
    11521 tataattaca cagccgttga atttagtact aatgttgaat agaagtacta atgctccatt
    11581 cagcacttct gtcactattt tggcaaaccc ttgttagcgg atgccctata gtctcgtaag
    11641 ggttctgctg ttgtatgtaa ttttcaacac gctcaattta ctatgtcttg cctaatatgt
    11701 tactgtttta attcctcaat ttcaatcttt cttcctgata gtcaattatc atcatttttt
    11761 tatgaataat ctcttcgtca atggtgggtt gtgttttgtt gatttgtaaa agttgttttc
    11821 gttgtaaagt taaaagctcg caataaatat gggtgctttt tgagtctaat ttaatttcgt
    11881 tgttttcgct taattgctct tgtacgttca ctaatatttc atgaaagcgt ttgctttttt
    11941 gttccttctc cggaaaaaca ctacgtaaat aatcaatcga aatcttcagc aattctttat
    12001 ccaaataagc cgacacctca tcttcaggct gcggatagtc tatctccttt agtttagctc
    12061 tttttattaa gaatggtaaa gtaagtcctt gtataaccaa ggtaagtagg atgacaataa
    12121 aggtaataaa aagtattaaa tttctttgag ggaaaacttc gccattgtcc aaataaaaag
    12181 gaattgataa agctgctgct aaggatacaa caccacgcat tccagcccat cctaaaataa
    12241 aaggaccttt aaacccggga ttttgtcggt ctgctactgt aataaaattt ctcataatca
    12301 ttgttacgcc aacagctccg taaaaactta atagtctaac taaaattacg actatggtaa
    12361 tcaaaattcc ataaccaata gcagttgtta tattcgtacc ttccaaaccg gctgtaattt
    12421 gtggcaggtc aagtccaata agtataaaaa caattccatt gagcaagaaa acaaagctct
    12481 gccatacatt agtacctcgt acccttgatg tggagctaag aaaatcatac cttttttgag
    12541 atacaaataa accgccagca acaaccgata aaactcccga agtgtgtatt tcttcagcag
    12601 atatgtacat taaataggga gctatgagtg ttaataacac atccatgttg gcatcggtag
    12661 gtaatttttt atgtgcttta acaaatatcc acccaaataa caatccacaa gctacgcctc
    12721 ctatcaccat ccatgcaaat tgtaaagatg ctttagacgc attaaaattt ccggtaacaa
    12781 ctgccagaat tgcaaactga ataataatta atgaagaagc atcattcaat aaactctcac
    12841 cttctaaaat cgtagatatt cgtttgggca attttacaaa ctttgtaata gcattggtac
    12901 ttacggcatc gggtggagaa acaattccgc ctaataaaaa tcccaacgcc aaggtaaatc
    12961 cggggataaa ccaataagcc gtaaatgcaa cgcttattgc tgtgataaat acaccaaaga
    13021 aagcaaaact gagaataatt cttctccatt taaacaattc tcgaaatgaa caagcccatg
    13081 ctgcttcgta taaaataggc ggaagaacaa gtataaaaat aagttccggt ctcagctcaa
    13141 ttgttggcat acctggaata aaactaataa gcaatcctgc cacgaccaac aaaataggat
    13201 atgctacacg aatttttttg gcaagcatca caatgccaat catcaccatt atcagaatta
    13261 gtaataatag aaatctatct tccatatatt ttagataaac acattagttt aattagtata
    13321 actttagtgt ctttggaaag tatctagttg atgtttttta tgggtaaccc tatttcagga
    13381 caagacacgg ttaaattgtt tctctttgaa gtaaactact gattaaggca cttttggttt
    13441 cgttatattt ttttgctgct cctacaaaat gaacaaataa tggagctggc tcttctacag
    13501 tgcttttaag ctctggatga aactgaacac caataaaaaa tgggtggctt ggaatttcta
    13561 ttatttccac taaacctgtt tcttcatttt taccacttgc tatcattcct gcttgttcat
    13621 acatctctaa ataatcatta ttaaattcat atctatgtct atgcctttcg ctaatttgac
    13681 tatttccata aattttataa gccaaagatt tttcttgtag cttacacggg taagctccca
    13741 aacgcatggt gccacccatc attttaattt tcttttgctc ttccatcata ttaataacag
    13801 ggcttactgt agttggttcc atttctacac tatgagcatc gtttaaacct aaaatatttc
    13861 ttccatactc aattactgcc atttgcatac ctaaacaaat gccaaaaaaa ggcaagctat
    13921 tttctctggc aaattgcact gcaaatattt taccatcaat acctcttgat ccaaaaccag
    13981 gtgccaccaa caaaccatct aacccttcta atttttctgc cacattatct ttagttataa
    14041 actcgctgtg aatattaaca acctgaattt ttacttcatt aattgcacct gcatgaataa
    14101 agctttctaa aatgctttta tatgcatctt gcaactctac atatttacca ataagcccaa
    14161 tagtaatttt tgctttagga tattttagtt tatctaaaaa tgctttccat ttatctaatt
    14221 gcgggtcgtt aaattgtttt atgtttaatt ttttcaagca aaataaatct aacttttctc
    14281 taagcattaa taacggaact tcataaatag tactagcatc catagcttct attactgcct
    14341 cttgtttaac attacagaat aaagcaattt ttctttttat atcatttcct aaaggttctt
    14401 ctgttctaca aacaataaca tctgggtgta ctccagtttc gctcaacatt tttacactat
    14461 gctgtgtagg tttggttttt aattctttgg ctgcttttaa ataaggtact aaagttaaat
    14521 gtaccaccac aacatcttca tcgggcaact cccattgtaa ctgacgaaca gcttcaataa
    14581 aaggtaaact ttcaatgtcg ccaactgttc ctcccaattc agtaattaca atatcatact
    14641 cgccagtttt accaattaat tgcattcttc tttttatttc atcggtaata tgtggtacca
    14701 cttgaactgt ttttcctaaa aactctccag cacgttcttt attaattaca gtttgataaa
    14761 ttctgccagt tgttacatta tttgactgtg atgtgaaaat atttaaaaac ctttcgtaat
    14821 gcccaagatc taaatctgtt tcggcaccat cttctgttac aaaacattcc ccatgttcat
    14881 aagggtttaa tgtaccagga tctacattta tataaggatc aaatttttga atggtaactt
    14941 taaaacctct tgcttgtaaa agctttgcca ggcttgctgc aattatgcct ttacctaaac
    15001 tacttgtaac accacctgta acaaaaatgt actttgccat tattttttta tttcaacaat
    15061 taaaaaatat tttttaaaag gcattataaa acaataattt actttaccat atacacagta
    15121 aaatataatt aatgaaactt gaataaagcc tgatatgacc ttgtgaaaaa tgtaaataat
    15181 aaaaagaaaa actttcagag gtcgtacaaa agtaaagtaa aattgaatag gcagttgctt
    15241 taacctataa aataattatt atttaattgc aaaatgtgga ataatactaa acaggataga
    15301 ttagctgatt aaataaacaa ataacattat ttttgcagtt caaaatttgt aaatgacaat
    15361 acatagagaa ggttatcaaa ccattggaat tactgcttta ttttttggag tttttaatgt
    15421 tgtttctttt tactttttta gtgcatcaat gccatggctt gcattgttag ttttttttat
    15481 tactctattt tttttattgt ttattatttc gttttttaga atacccaata gaaaactaac
    15541 tattgatgaa aataaaataa tttgccctgc agatggaaaa gttgttgtaa ttgaagaaac
    15601 aacagatgtt gaatatttta atgacaaaag aatacaagta agtattttta tgagccctgc
    15661 caatgtgcat gtaaacagaa accccatgag tggagaggtt ttgtataata aataccataa
    15721 aggcaaatat cttgttgcct ggcacccaaa aagctctaca gaaaatgaac gttggagtgt
    15781 ggtggttaaa aataattttg gcaccatact ttacaaacaa atagcaggag cacttgcaaa
    15841 aagaatatgt aattatacac aagtaggcca acaagtaaac cagtgtaaag aatatggttt
    15901 tataaaattt ggaagtaggg tggatatttt attacctatt ggtacaaaaa ttaatgtaca
    15961 attaaaccaa aaagtacagg gcggtgttac tgttttggca gaatggtaat ttttataaaa
    16021 atttatttac atatatttta tacttatttt cagatagaaa ttttattttt ttgtagaata
    16081 ctattttttt tattactatt aacgtttttg tatttaatat tctcttattt ttacaccatt
    16141 aactctttaa taaaacctaa aaatacaaat catgaaaaaa tttttaatgt tagctgcatc
    16201 cgtaatatta tttgtaggtg tagcattagc ttgtggcgat aagaaagact gcaaaaaagg
    16261 aaaagattgt tgtcaaaaag aagctaaagc aaaaaacaaa gaagcttgca gtaaagaaag
    16321 taaagacaaa gtttgttgca aaaaagatgg tgcagaaaaa aaagaagtaa aaaaagaaga
    16381 aaagaaagaa acaaaaactg agaaaaaagc ttaattaaaa gaatacattt aaagagcctg
    16441 tcataataaa atgacaggct ctttttttaa ttaaaactga acattaccaa ctgcttaatc
    16501 ttaacccaat tgcataaggg tactgattta ataatgttgc tttattatgc aaagcattta
    16561 aactataact ggcataaagt cttacaggtc ctacaccaac ttcgccaaca gcagctaaac
    16621 gaaaaggttg taaatcaaaa tcgcctttgt attttatttt tcctctggta tcgcttactt
    16681 gtttgtttct tgtactaatt aaatatcctg cacttactcc tgcactaaaa ctaaaacttt
    16741 ttttagaatt gggcttggta ttaatattaa ccattaaggg taatgtaata tagcctgcgt
    16801 ataatttgtt ttttgaaaaa ttgatggagt cattaaaaat aaaaggatta ggatcttttc
    16861 tataactaac atttctgtca aacctaaaat tgtacatctc taaacctaag ccatacttta
    16921 aattaaatac atgctggcta atatttaacc tttgcataaa aaaccaaagg tttacgttag
    16981 agcttttgcc agttttaagt cttaaacttt gactggttac tgcacctgct ggagtgtata
    17041 aagttcttaa ataactacct gcctgtgcat tggtataatt agtttcatct ctataattag
    17101 caaagcctaa atctaataca aaccaattgg tagaaatatt tttgttttta tcttcactat
    17161 tatggatgga tattttaaac cctttgcttt ctttattgcc tgttttgcca ttctttttta
    17221 caataaaaaa atttcctgcc ttaactgtat cttctgtgtt ttctttactt tctgtttgag
    17281 caaatgtgat gtatgcagca cttaagagaa ctgctgtaag tatagttttt ttcataatga
    17341 aatttagtat aaaattttgt aaatagttat aaagtggcta aggctattgc cttattatct
    17401 ataggtgcac caattatttt ctttgctttt ttaaaaatat tttttagctt acttttttta
    17461 acttttatgt taccaatatt aattagtttt tcattgctgc tttcgttttc tgtaatctca
    17521 tttatatcta tatattgaaa tgtactttgt tgtaccatat tggtttcctc tatattattg
    17581 ttaacttgaa ttaatgtatt taaagctgta ttattagaag tttgtagtgt tttataattt
    17641 gcattctgat atacactctt taagtcaact tcaactatgt tttgttcttg tttggtagtg
    17701 ttgtggatgg ttgaaactgt tgaattagtt actgcattat ttacctcatt attttgatta
    17761 ataattgttg ttgcaagatt agtattagaa gtgctttgca ccttaggctc ctcttgttgg
    17821 ttatatagtg tagaaggctg aactttatta ttagatatag taggtatgct tgtttttgct
    17881 tgtaagttat ttattgaaga ggtgttattg ttttgagaat tattagtttg gctgttaaaa
    17941 gcaatattat tgttattgat aattgtatta ttaaaattta atagccaaac agctacagct
    18001 attaatacta aagaagcagc tatagcaaca ggtttaaatc caaaatagat aattggcttt
    18061 ctttcatttt taagtaactc atttttaaaa gggcattgaa tattttccgg ttgaagagtt
    18121 gccattttta atacagtaaa ttcgttttga taacttggat tttgcaaaac aaatttttca
    18181 actgcttgcc ttttattttc atctaattca ttatcggcat ataataaaaa attttcttca
    18241 tagttagctt tatggatggt atctatatca aatctgtaca aagtgctttt attagcactt
    18301 attaaggttg ggttaggtaa tttagtttgt ttaaataaat taaactctac ttttagatct
    18361 ttgtgtatta ataaaaattc atctaccata tttttttgat gaacagatag ctcatcatct
    18421 aaataaagta aaaaatattc ttgataattg tttctattaa tttccatttt cattgcttca
    18481 tttttctact aaaaacttat aacacatttt ctctttttac taaaaaatct cttagtataa
    18541 ttcttgctct atgcaaataa acttttacct gactggcatt taggttagta atttttccaa
    18601 tttcctcgta gctatatcct tcataatctt ttaacataac taaacttttt tgtatatcgt
    18661 ttaattttga taaagcttca tcaaaaacat ttttagaaaa gctatcggtt gggtgattta
    18721 ttcttacatt gtctttaaaa ctttcggtta aaacaattct tttatttttt ctaatatggt
    18781 ctatcatttg attgtaagct atagtaaaca agtatgattt tgatttatca gtttcaacat
    18841 tttccttgtt tctccatagt ttttcaaatg cagtttgcac aatatcctta gcatcttcct
    18901 cttgcttaat attttttaaa ataaacctat acacattatc tgcatattgg tttacacaat
    18961 tattgtaatc tctttctgtc atgcaacagc taaggtagtt ttgtttaagt tctaattaat
    19021 gtaagtaata cgcaaaaggt acaataatgt tacacttggt aaaaattaat tttaataatt
    19081 tataacctgt tcttgtataa agtttggcaa atctctccat tcatattgtt ggttgcgtaa
    19141 ttgaggtcca aaaccagctt cggaaatggc attttgaatt gttttataag taaatctatg
    19201 aggggcaccg gctgcactta ctacattttc ttctaacatt atacttccaa aatcgtttgc
    19261 accgccatgc aaacatattt gtgcagtttc ttttccaaca gttagccaac ttgcctgaat
    19321 atttttaata ttgggtaaca ttatacggct aattgcaata gttctaatat attcttcgga
    19381 agtagtgaga ttatgcactc ctctaatttt agctaacaag gtatctacat cttgaaaagt
    19441 ccatggtata aatgctaaaa aaccttttgc agaatcgggt ttcttagctt gtatttctct
    19501 tattttactt aaatgaataa atctttcttc tactgtttct acatgtccaa acatcatagt
    19561 tgcactggta gtaatattta gcttgtgggc ttgatgcata atttccagcc attcatctgc
    19621 cccacatttt cctttactta ctaatcttct cactctatct actaaaattt cggcacctgc
    19681 acctggcaaa ctatctaaac ctgcattttg caaagctttt aaaacttcgt agtgggtact
    19741 tttttccagc ttagtaatat gtgcaacctc aggggggccc aaagcatgta atttaatggt
    19801 aggaaattct tgctttatct ctctaaatat ttttgtataa aaatctaagc ctaagtttgg
    19861 gtgatgccct ccttgcagta atagttgatc tcctccataa ttaatggttt cctgaatttt
    19921 ttttctataa gtgggcatat cggtaatata tgcttctgaa tggccaggta ttctataaaa
    19981 attacaaaat ttacaattgg caatacatac attagttgtg tttaagtttc tatcaatttg
    20041 ccaagttact ttaccatgtg gcacatggtg ttttttcatt tcatttgcta tatgcatcaa
    20101 ctctgttaaa ggtgcatgtt taaacaaaaa cataccttct tctatactta aaaattcaaa
    20161 ttgtaacgct ttgttatata aatttgataa ttgcattatg gtatttataa gttagcaaag
    20221 ttaaggtgta taggctttgc aataaatggg ttagttattg aaattgaata ttgttacatt
    20281 ttatttttct ttatcttgta actagtttaa aaaaaaatac tgcttttgat tttattaaga
    20341 catataccat ttttaaaagc tgttttttta tatagcatta ctgcctttgg aggaccacaa
    20401 gggcattatg gaatgatgct taaaactttt gtaaaacaaa gaaaatacgt atcggaaaaa
    20461 gagcttttag atttagttag tttttgtaat atgattcctg gtgcatcatc tacacaaact
    20521 attacattaa taggttataa acgtggtgga acaggtttgg ctttattaac tttattaatt
    20581 tggatttttc ctgcaagcgt tttaatgggt ggtttgtctt ttttattaag ttatttagac
    20641 aaaaattcaa ttaacactca atggttcagg tttattcaac caatggcagt tgggtttata
    20701 gcattttcat cattaaagct ttataaggtt tgtattaata acaccattac aagaattatt
    20761 ttagttgctg catcatttat aacatttttt gcttttaaat cgccttgggt ttttcctgtt
    20821 ttaattattt gtgcaggaat agcaacaaat tttagcgata aaagaatacc tcaaaaaaca
    20881 ttacctacac aaaaaataaa gtgggcaaat attttaattt ttctttttct atttggttta
    20941 actgggtata tatcggaaac tgcaaccagg caaaattggg aaaacagaaa agccattaac
    21001 ctatttgaaa acaattacag gtttggaagc ttagtgtttg gcggtggcga tgttttgatg
    21061 cctatgatgt atgagcagta tgttgttaga ccaaagacag agaaaatatt gaaaagtaaa
    21121 agagatgttt taaaaatgag taaagaagaa tttttaacag gaagtgggat ggtaagagct
    21181 ataccagggc cagtttttag tattgcctct tttacaggtg gtatgttgtt aagaaatgaa
    21241 ggtagcccat tacttcatgt tttaggttgt gtgctgggtg taataggtat ttttttgcct
    21301 agtgctttat tggttttatt cttttttcct atatggcata acttaaaaaa atatgctgta
    21361 atttttagat cgttagaggg tattaatgca gtggttgtag gaattatggc aggtgcattt
    21421 ttttatttag caaaagatat tttatttacc gatgtacaaa tttcgttaaa gaattttagc
    21481 ttagatgttg tagtaattat agctactttt ttaactcttg aatatacaaa aaccaaagct
    21541 ccttttattg tatttgcctg tttgctttta ggatggttac tgtagtttat ccattaacat
    21601 actgctgtac aggaagccta ttttgtaaac ttccagctaa tgtcatttca tcggcatatt
    21661 ccaactctcc tccaaaagca ataccacgag caatagtggt aattttacaa ttagtatttt
    21721 gtaatttttt ctgaatataa tagatagttg tatctccttg aatattgggt gatagggcaa
    21781 aaacaatttc ctctacttgg tcattttcta ctcttgaaag taaaccatta atatttaatt
    21841 tatctggtcc aataccatct aatggagaaa taacacctcc taaaacatga tacacaccat
    21901 tgtattgttg agtgctttca attgcaatta catctctaat attttcaact acacaaataa
    21961 tttgttgatt acgcatgcta ttactgcaaa tactacaaat gtctccatcg ctaatattaa
    22021 agcaacgatt acaaaatttt atactattac gcattgtata aattgtatcg ccaaataact
    22081 ttacttcttc ttcattttta tttaataaat ggagcactaa tcttaaagcg gtttttttac
    22141 caatacctgg tagttttgca aactcagcta cagcattttc taataaaaca gaaggtaatt
    22201 gcataataca aagataataa ctgctaattg ttagccttta taaaaataat tttacataca
    22261 accaaataaa tggtactgct actagcaatg aatcaaatct atctaaaaaa ccgccatggc
    22321 ctggcataat gtttccacta tcttttacac cagccattct tttaagtttg ctttccagta
    22381 aatctcctaa agtacctgta atagatgcaa ttgctgccaa tataaaccaa tgtactttta
    22441 agctagcact ttggccaata agttgttttt cttctataaa ataattcatt gcaaaaccca
    22501 taactattat acacaataca attccgccaa tggtaccctc ccaagttttt ttaggagaaa
    22561 ttttagagaa aggtgtttta ccaataaatg agccaactat atatgccatt gtatcgttta
    22621 cccacataca tactataata aaaattgcaa taataccatt gcctattata gcgttattaa
    22681 tatttatctc gtacaagtct aaaattaacc caatgctcat ggaaatataa atcaagccca
    22741 aaaacgataa aaaggcaggt ttaacttctt gttttttact gaataaaatt tcggttatag
    22801 gaagtacaat agcaaaaata actaccaaac tcattcctaa taattgcagg ttaaatgacc
    22861 caaaagcata acttgcattt aacatccaaa gcataaaacc aaagcccgta attattacac
    22921 catatttgtg aaaaggtgta attgttttat aacaatcatc tataagcgat aatagttttt
    22981 gatattctat ccaacaccca aaatgtataa tggtaaagag taaaaaaaat gtccagttgt
    23041 ttacaaataa cccaaacaac attattgcag caaaaacaat tgcactgagt gtacgtgttt
    23101 taaatgtttg aatattaaaa gccataattg ttggaaacta ctgtacgaat ttgcataaaa
    23161 aatttaatat cagtttaaat attttaatcc tttgcattat acataaaaga aaaggatgca
    23221 acaaagttag tttaacaacc ttgttacatc cgtagtagta cacattttta ttatgtttac
    23281 tatagtttct atttatgctt tagcattttc ttttcctaat aataatactt catttacttg
    23341 tacttcggta atgtattttt tatttccttc tttatcaata taagacctat taattagttt
    23401 accttccatt gccatttcag ttcctttttg taaatacttc tcaacaatat cggctacttt
    23461 accaaaagct acaatattat gccattgggt ttctactact ttttcgcctt ttgcatttct
    23521 aaaaacttct ttggtggcca tgctaaaacg agccattttt ttaccagttt ctaatgtttt
    23581 tacttctgga ttattgccta agttgccaat tagttgaact ttgtttttta gtgcgtacat
    23641 aattttaaga tttttattct taatttcatt tttattggtt ttgaaaaaaa gaatggttta
    23701 ttaatttatt tcaatcaaac aaagtagtat tgacgtctga tactttttta tttgataggg
    23761 caaagatgat tagggtaaaa aacaagactc ggtttttaag tgtttactct cgatgttaag
    23821 cgtttttaaa cgactataca cgaatattta attgtacatt ttagttgtaa atcaaagtat
    23881 ttaataaaaa atagaaagca gaaaattgaa aattattttt agataataaa ttatttttta
    23941 tggagcattc aatagtaaaa aaatgccttt cttgtggtaa agttgtaaaa ggcagaaccg
    24001 ataaaaagtt ctgcgatgat tattgtagaa ataattttaa taaccagaaa aaaagtaaag
    24061 agaattatag cccctatgta aaaggaattt taaatacatt attaaaaaac agaaaaattt
    24121 tagagaccct tttgccacca gataaacaaa ctatccattt taaaaaagaa aaactggtac
    24181 aagcaggttt tatttttaaa tattttactc atacttacat taacaaacaa cgtgagacct
    24241 atttttattg ctttgatttt ggttataaac aactagaaaa tgattggctg atgattgtaa
    24301 aaagaagaga aggagatgaa agaagtatat aaatacagtt acaaaaaacc aataataagc
    24361 agcaaaatat aaacaatatc tctgtatttg tattttaatt gttcttaaag aagatattag
    24421 gtagcaatat tatataaaaa aggttggtat aaacaatacc aacctttgct ctccctgttg
    24481 gacttgaacc aacgaccctc tgattaacag tcagatgctc taaccaactg agctaaggaa
    24541 gaatattttg atgtaagata agaactattt tggatattat ttccctttcg gggcagcaaa
    24601 aataaggttg tagtatttga aagcaaaatt tttttgccgc tatttaaaaa aatgagagaa
    24661 aacctttgtt ttctatggca ataaatacac cttcttctct tatttctact ttataggttt
    24721 ttaaaaaata cccttcgcca ctcgtatttc taccattttg catacaaaat ttatatctat
    24781 gtattgggca aacaacatta gccaaagcat ctatccaacc atttgctaaa atgccacttg
    24841 catgagggca tttgtgcgaa aaggcaaaaa ttttattgtt atgtttagca attgtatata
    24901 ttttattttt tacctccacc aaacacatat tatttgcttg ccatggtaac aagttaattg
    24961 aatctgcaat tttataccat tttatatctt gcatattctt ttaataatga atagttttat
    25021 acggatatct aatttgataa atatctctta ctttatttaa aataactgct ctaagttcat
    25081 ctatattttt tctttctaat gccgaaataa atacacaatt attatcggta ttgtttaccc
    25141 atttttcttt taattccaat aaaatatctt gttttacttc gctatgcaac cattcatcaa
    25201 aatatctttt ttcatacaaa tccattttat taaaaatggt taatacaggt ttatcaaaag
    25261 cttttaaatc ttgtaaggtt ttatttactg tgccaatttg atcttcatat tgtgggtgag
    25321 caatatctac cacatgtaat aaaatatctg cttctcttac ttcatctaaa gtgcttttaa
    25381 aactttcaat taaatggtgt ggcagttttc ttataaaccc aacagtatcg cttaataaaa
    25441 atggggtggc ttcaaaaaca acttttcggg tggtggtatc taatgttgca aataatttat
    25501 tttctgcaaa aacatcgctt ttacttagca ggttcataat ggtgctttta cccacattgg
    25561 tgtaaccaac tagcgatacc ctaattaatt ctcctctttc ttttctttga gtgaaagatt
    25621 gtttatcaat ctcacctaat ttttttctta ataaagaaat tttatctttt acaatacgtc
    25681 tgtctgtttc tatttctgtt tctccagggc ctcttgttcc tatgccacct ccttgtcttt
    25741 ctaagtgttt ccacatgcct tttaaacgag gtaaaatata ttggtattgg gctaattcta
    25801 cttgcacttt tgcttgagct gttcttgctc tgcttgcaaa aatgtctaaa ataagatcgc
    25861 ttctgtctat aatgggtatt cctaaaactt tttcaatatt tacaagttga gagcctgtta
    25921 gttcatcatc aaaaattacc atatcaatat ttcttccttc aatatagttt ttaatttctt
    25981 ctaactttcc tttaccaaca aatgttttgc tatcggggtg ttgtagtttt tgagtaaatc
    26041 ttttaactgt aagtgcacct gcagtttctg ctaaaaaagc aagctcatcc atatattcat
    26101 taacttgctc tatggtttgc tctttttgta ttacacctac taaaattact ctttcttgtt
    26161 tgtttgtttt ttgttgtttt tctatcaaaa ggttttagtt ttttatgaac tgcaaaatta
    26221 gatgaattgt ttgcagctat taacatatta aacaagaaaa taaactttat ttaagttgag
    26281 aagaaatgaa ccagcttatg ctttttgagc aataatagaa atagcattca tttggtcttt
    26341 gtatttatta aacacttctt tcattacaag tatgtgtttt cttgctttcg gaaatcttaa
    26401 aatattaaac ccaattttta aagttctaaa taatccttca tcatcaataa ttcgtttggt
    26461 ttctaaaagc aacattgggt tagtgtttat ttctacaatt ttaaatcctt ctttttctaa
    26521 aagatgtatc cattcgcttt gtgttaaagg tcttgcatta actcatatac aagtggcaag
    26581 ttcatgctga atttttgctt ttgtatcttc attcaaatta tctggagtta ggcccaattc
    26641 atgtatgcca tataaaccac cttttttcaa aagcctgtaa gcttctttta taatttcaga
    26701 ttttctatgg tctgcttgca tggttagcat tgcttctgca taaactttat ctgcacttgc
    26761 atcttctaac attacattag aggcattacc aataataatt tttctgttgt tgctgtttat
    26821 ttttcttttc aaaagtgtag cggcttcttc atttagttct acacctgtat aagattttgg
    26881 attaaacttt aaaaccttca tagctgtata acctatacct ggtgcaaact ctattacatt
    26941 atcactatta gatattttta agcccttaat tagctttagt gttaattctt tacctccagg
    27001 ccttagaact tttttgccca tttttgctaa cagccaatgt ccttgctctg tattaattat
    27061 tttattactc ataaaatttt aatttgtatt gaaagctaca aaattcattt acaagcaatg
    27121 ctattattat ctaaataaga aatttgattt attgaatgag gaatatcatt tttaatatct
    27181 tctgaagcac ttgatacaag tgcattacaa gatgttttat ttttttattg taaaatattt
    27241 ttttaaaaaa tgatgacaaa tttatgacag gtattgtgct gtaaattttg tgcccaataa
    27301 aaccattatt cctaatataa aaactatttt tttatataat aatattcata aattttaatt
    27361 ttcaatactt ttttcatgta gaagatttta tataattaag tattgaagaa aaattttaca
    27421 gttaaaatac tttattgttt aatacttagt ctgcttaata accaatggct tttcaaacat
    27481 tgttgaacac caatactttt cccatcttgt ggtttgatgg gtaaagcata aacaatttta
    27541 tcattaatca atacagttaa agtatcattt ctttgaatga tttcatattt atatagaata
    27601 ccatactgca atgtataatt taatgtttca gtataactat cagaccaatc ataatatgtc
    27661 caaccattaa tattatcaaa ctttgataaa cgcatatctg ttagtccaaa atcaagttga
    27721 tacagagatg tgctgatgct tgatgaagta ttggtatctt cttctatttt accccattga
    27781 aaatcgaatc cattagaaac atcgtcagag ctcaatatct tagcgtaaaa agtaattata
    27841 aaattttttt ctgctttaaa cttccgaata tttttttcaa accatagaca accattatta
    27901 acagcgtttc tatgattctt tttaaaataa agtgctttat ctttaattct tacagaaaat
    27961 tcgtcatctg ctttttccaa tttccaacga ttcttattat taacaaagtt gtccttaaaa
    28021 attatttttt tttgtccata taaaacaaaa gaaattaaga tgcttaataa gaacattgca
    28081 tttttatatc ccatattgtt tgctatattt attatcgcca tagaaaaata ctacaacttc
    28141 tattctaaaa attttgtgta gagccaacaa gtgcgtactt ttcatgctca aaaatataca
    28201 actatggtta taatagcttt ttataattta aaaggaggag ttggtaaaac tgcttctgca
    28261 gtaacctttg catacttagc agcagccaat ggtttaaaaa ctttggtatg ggatttagac
    28321 cctcaagtgt aatttgaaaa tttttatgca gattcaaaaa ctgcttatga aaaactactt
    28381 gcttcaaaag atgcagaaat agtgcgactt accaaacaaa tagaagattt aatgaaactg
    28441 ttggggaaga agtaaaaagg aactaaatat tcttgacaaa ctattacaat tttattttct
    28501 tccatttttt ataacttttt gtacatttgg caaatgggaa caacagcaga aaaaaatact
    28561 acgcttatag acggttatgt tggtatgctt gttaatttaa gcactaccaa caagcttgac
    28621 cttatttcta aacttacagc ttctgttaaa accgacttaa caaacaaaaa atcgtcattc
    28681 aaaaaagcgt atggtgcttt tgaatcaaaa aaatctgcag aagaaattat tgaagaaatt
    28741 cgtaacagca gagtttctac aagacaaata gaagcatttt gaaaaagtat ttgattgata
    28801 caaacattgc cgttttttat ttaagaggca agcataatct tgaatcaaag tttgaccaag
    28861 ttacggcaga tgattgttat atttcagaaa taactttggc agaattgaaa tttggtgttg
    28921 aaaaaagtga aaaacctgaa aagaaccgaa aagtattgga aaacttttta actggtgttc
    28981 aagtacttcc tattttccat tcattagatt tgtacgcaaa agaaaaagca agacttcaaa
    29041 aagcaggaac gcctattgac gattttgatt tgttgattgg cgtaacttct ataacacata
    29101 atttgacaat ggttacaaac aatacaaatc atttcaaaaa tattcgtgaa attgatttgg
    29161 aagattggac acaagcctag tttttttctt cttcctaact aaacaaatac tctaaatctt
    29221 cttgttgtaa gctttccgaa gattagtacg atttgaaatt acaagatgtt ttattttttt
    29281 attgtaaaat atttttttat attttttagg aaaaagttta aatactagta ggtgtattta
    29341 ctcttatact tacacaatgg cagaactata ataaaaaaga aattttagat taaaattaaa
    29401 ctacaactta ttctattcat ttcaaaaatt agcttaaaaa aacagaaccc tgcactaatt
    29461 aaagtacaag gttcttttat tcccaacttc aaccaacttt aaattattta tataccaaat
    29521 tttattaaaa tatttttaga aatagcattg ttatttggta tttgtactgt tgatttagta
    29581 ggtgtgttgg atgttttagg ggtagtaggt tcatacctat attttttttc atctaaaccg
    29641 gtgctgtctt ttgtgttatt atctaacttt tgttttaatc tatcggcttt ttgttgtgcc
    29701 tcttcgtatt ctttttgtaa ataatctttt tcattcattc taaaatcatc gtcatcgtcc
    29761 tcatcgtatt tattattggt acgttttaac ccttcattgg tcattatgta ttctacatta
    29821 tgtttccaag caagttcaga gccatctaaa ttataccaat caaaatctga atcgtcgaag
    29881 aagctaacat gaatatttct attccaactc catcctgcat ttcttttaat aattattttt
    29941 ttaccaattg gtacggctat actaattatt acattttgat ttctaaattt atcttttggg
    30001 ttaatagtta tccctcttcc taaatacaat agtgtatctt tttgctcaat agaaaagttt
    30061 attttttgtg ctaactcatt agcttgtttt ttactgttgc cattacttag ttttaccatt
    30121 tttaccgaaa aactatcgtt ggttgctttt gtaattctta ttcttacatt ttttattaca
    30181 ctgctatctt catctacatc tgcaaatggg gccatatcaa accaattatt attgtagtaa
    30241 ttggttaagt tatttggatt aatttctaat ttatttactg taggattggt aagtaaaact
    30301 tgttcttcca tagcattatt tctgtattta aaatctttac ttaaagaaac aagtaagttt
    30361 ataaagcaaa ccaaacctaa tacccatagt gcagagaagg tatatcttac caaacaactt
    30421 ttacctttaa tttttgccaa tctgcgtact ataaaaacaa ctatagcaat aatgggtacc
    30481 caaataaata gtaacaatgt gccccatgca aaaacatttt gccaaccagt tgaaataata
    30541 taacctttaa gtggtaaaaa tcctgatgct acaataccta acccaaataa tgatacaaca
    30601 attgccaata acactgagcc taaaataaaa tatgcaaata tttttacaat aagtacaatt
    30661 atatcgccca agcctttacc tcttttttta aaaattgtat ttgcctcggt agaaaattgc
    30721 ttcccttttt cttgaactgt gttaccaaac tctgctcctt tttcgctaat tgtttcgcca
    30781 aatttttccg ctttttttgc aaaattttca atatcgcttt gaatagtatt ttttatatta
    30841 tttaaatcaa ctttttcacc tttcatttct agtttatcgg cactagattt tgcttcgggt
    30901 aaaatcatcc ataaaataat atatataaat aatgctcctg ggctaaaaga tatttttaag
    30961 aaattaggaa aatcccaaat atcccaagta ttaaaacgaa atatgaagga aataaacggt
    31021 attaaaaaaa gcaatctcgg aatccaaaca cttatcccaa aataatgact gataccacta
    31081 caaacgccag ccaaaatttt atcatcagga tctctaaaaa gtctttttct tctactgcca
    31141 attacttgag tggaactgct tggaattgca acccatagaa taatgtaagg cacaaacatt
    31201 actccaaaaa atatcacaaa aattacacgg gttaccaccg gatcaatacc aaaataatta
    31261 gctataccac tgcaaacacc ccctaatact ttattgtttt catctctata catttttttg
    31321 gtatcgcttg tattactata agagttttgg tgctcatagt tttgatgctg ttgcttatcg
    31381 gcattaggct gcgaaatatc ttcatcaaaa tcttctggcc tacccatgct attaataata
    31441 gtatttacat cctctgctgt aatgcaggta gaaccttttt tcaatacttc gccaaaaagc
    31501 tctgcaattc ttccttctat atcatttatt atttcttctt tgccctcttc gttagcaaaa
    31561 agtttaccta agctttcaat gtatcttttt aaaatatcgt aggcattttc ttcaattgga
    31621 attactctgc cttgaaagtt tatattaatt acctttttca tactttgtaa ggtttatttg
    31681 gttaaagtta gttttatgag ttgttgtttt gttgggttaa agtggccact gcatctgcaa
    31741 gttcttgcca ggttttgcct aattcgttgt aaaagtcaag acctttatct gtcattacaa
    31801 aatattttcg gggtggtccg ctattgcttt caacccatct ataagttaaa tatcctgcat
    31861 tttttaatct ggttagcaaa gggtataatg tgccttctaa aatttgaagg ttagctgctt
    31921 tcattttgtc tgcaatatca cttgggtaaa cttctccctg gcgtattata gataaaatac
    31981 aaaactccaa tactcctttt cgcatttggc tttgtgtatt ttgaatatcc attatagtaa
    32041 tattaatagt taaaaattta ataccggtgt tttgtaatag aataaagtat taccaaataa
    32101 atgctgcacg gcggatagga gtaacagatt ttctagatct tcttaaattc cataaatctg
    32161 cagaactaaa aactttatta ttattgttat cagcaactgc cacaatttca ttttgagtgg
    32221 tagtaagttt aactatttcg ttgttagtta aaggttgtaa aagatttgtt tgttgagttt
    32281 tcattgtagt atttttttaa ttttttaaat tttttgtttt tttaatgttt tgggggttta
    32341 tacccttttt ttacattgac aacacaaaga tgggattttt tttaatacta tgcaatgcat
    32401 aatactatcc ttatttaagt agtttgccaa ggataatgat aaaaatagct ctgaaactca
    32461 atgtttatag gcattcaacc aaagccttac atggtactat gcaatacata gtattatgat
    32521 gagcggtaaa atagcatgat gaaaattgca aaattttact tgaaaacata aaaattttaa
    32581 attttggaag tagaatttgt aagaaaaaaa cataaaaact atctaattaa gaacaatgct
    32641 ttagtttcgc tgccatcaaa aaataaaaat tattaattta tgtctttagt tgtggttggt
    32701 tctatggctt ttgatgcaat agaaacccct tttggtaaaa gtgataaaat aataggtgga
    32761 gcaggcacct atattgcttt ttgttcaaca tattttacac ctgtaaaaca aataagtgta
    32821 gtaggtggcg attttccaga agatgaatta gatgaattaa gtaaaagaaa tgtttgttta
    32881 gaaggagttc aaattaaaaa agatgaaaaa actttctttt ggagtgggcg ttaccacatg
    32941 gatatgaata gtagagatac cctggttaca gagttgaatg tattaggaga ttttaaaccc
    33001 atagttcctg aaagttatca ggattgtgag tttttaatgt taggtaattt agcccctgca
    33061 gtacagttaa gtgttattga gcagcttaaa aacaaaccta aactaattgc tttagatacc
    33121 atgaactttt ggatggaagt tgccatggat gatttgaaaa aagttttaga aaaaatagat
    33181 gtattaatta ttaatgatag tgaggcaagg caacttagtg gtgattattc aatagttaga
    33241 gctgcaaaaa aaataatgac catgggaccc aaataccttg taattaaaaa aggtgaacat
    33301 ggagctttat tatttcacga aaacaatgta tttgtagcac cagccttacc attagaagat
    33361 gtttttgatc caactggtgc aggagatact tttgctggag gatttattgg gcatttagct
    33421 aaaacaaaag acattagttt tgaaaatatg aaaacagcta ttattgtagg tagtgcaatg
    33481 gcaagttttt gtgttgaaaa atttggtact gaaagattaa gaaatttaag caaagatgca
    33541 attgcagaaa gaattaatga atttgttcaa ttagtacatt ttgatgtaag cctagtttaa
    33601 tttattattt acaaataact aaaaatccgt agagtagctt tacggatttt tttattacat
    33661 ttttccaaac attctgtcta aataatcttc tttaaactct atataagttg gattacctgt
    33721 gggtgtaaca tttggagttg tacattgaaa aagactttct aataaatttt gcatttctgt
    33781 aatagataag gttgtacctg cctttattgc ttgttgccta ctaatacaac gaactagttt
    33841 ttctctttta ctgtatttta tatcgctact aaagtgttta aattgttcta gtaaaagttc
    33901 taaagagtgt ttttcatttc ctgtaccaac atctgctggg gtgccttgaa taataaaagt
    33961 attgttacca aaaggctcta tttgataacc aatagtaata atttcgggca ttaaatcttc
    34021 taacaaaata gcatctgggg ttgctaattc aaatgttata ggaaaaagat ttttttgagt
    34081 agcaatttgc ttattgtgag aataggcaga atatttttcg tacaaaactc tttcgtgtgc
    34141 atgttgctga tgtactaata aaaatccagc ctggttagtt gttaatatat atgttttatg
    34201 taattgaata aataaacttt caacattttg taattggttg tattgaattt tttgctccac
    34261 ttcgggttca ttgtttattt caacaattgg ccgagtagta aaaaaatctt tccaatgttt
    34321 taattcactt ttttcgtttg gttctatttt atgtgcctgg tgtttaccag taaatgtttt
    34381 aaataggtta gaggcagtag cttgttgctt atgctcggtt gtaaagggtt tatg
//
LOCUS       DAOQPK010000002        32264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_611_length_32264_cov_4.676674, whole genome shotgun sequence.
ACCESSION   DAOQPK010000002 DAOQPK010000000
VERSION     DAOQPK010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 32264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32264
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_611_length_32264_cov_4.676674"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PLU36_00185"
     CDS             <1..501
                     /locus_tag="PLU36_00185"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:NF023246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HMZ45196.1"
                     /translation="VVPDIVMPDTYEYLEFREKDNSNSLPWDEIPKADYAVDSNSNEL
                     NFIISSENKMISENNNLNIIHNNALWLANNTKKPISLNFDNYKIFQKQIKSTVAQTTT
                     LSKLSKEMDVTVTSADKSKYFSNVDKAKGDRYQAWLKNIKNDMYISTTADMIVNMANK
                     KIVYNY"
     gene            668..1444
                     /locus_tag="PLU36_00190"
     CDS             668..1444
                     /locus_tag="PLU36_00190"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HMZ45197.1"
                     /translation="MKITRIIALVIAVSMFTTSCTNSKKLVILNDIPDTARVALPFIE
                     TPVNTIQPDDILEIKISGKNPETSNDFNSKGGGYYLNTGASSAAVPNYLVDKNGFIEI
                     YKLGKIKAAGLTKDQLKENLQSALEKELVEPTVVVRFVNMRFTVLGEVRTPSTYTIPN
                     EKLTILEAVGYAGDLTMYAKRNSIRVIRDSSGHREIGSINLNQKSLFTSPFYYIKRND
                     VILIESDYTSKKQTEVFAKTASIIGILSGLITVVYLILRK"
     gene            1453..>3725
                     /locus_tag="PLU36_00195"
     CDS             1453..>3725
                     /locus_tag="PLU36_00195"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HMZ45198.1"
                     /translation="MSYPTKGYEQEVVQQQASNNQLDAKKLIYKLIGILPWVIISLAI
                     SIFAANLYLRYTPKLFRVSGNLLIKDEDQSSYRSLKDLGIIPGNIDVPNQMQILNSYT
                     LTERVIDSLKLRLKLRQEGRVISSELYGKTMPFKINVVEEYPNTKASAYHINLTRENF
                     IISGAEGQKKYTFGDTVDISYGKIILERNPLVKINPNGYQLTINDLESEVRALRSKIT
                     VVQLTENGGILEIATNEELPDRGIDIINKLMEVYDNAGLDDKNIVAKKTIQFLNDKLG
                     NIAKDLDSIEIKIEQFRKDNKVPSFSEAGSAFFAEYTGLDKNKKILVTQSAMLDHLES
                     YITNFRNTNDIIPPTFGQTDVILLGLIETHNTTVLAKQKLELISKPTDSHLQNMIESI
                     KGIRQNILRAIRNIRVANQTQLQNYESNYSLLESKLQSIPEKQRIEMRLERDRVVKQE
                     IYKDLLKRKEETELSLASNINNTRIVDYAQNYGPKSPNIATARSVAILFGILIPILII
                     VIKDFFNTKIQDRKEIEQATAIPILGELSYEKDKKNVVIENKSRSPIAEQFRLIRTNL
                     QYMVPDKPLQSLLVTSFMSGEGKSFVSLNLASSLAITGAKTVILEFDLRKPKLSKYLE
                     LSNDIGLSNFIIKDMNIDEIIQEVPNRENLFVISSGPIPPNPAELLLSQRTVKLFEQL
                     ATRFDYIIIDTAPVGLVTDALLLEKFVDLSLFILRHKYSFKAEIPFVDKLYRDKKFKN
                     MGIIVNGIKNEGSGYGYG"
     assembly_gap    3726..3825
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3826..4013
                     /locus_tag="PLU36_00200"
     CDS             <3826..4013
                     /locus_tag="PLU36_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45199.1"
                     /translation="MGIIVNGIKNEGSGYGYGYGYGYGYGYGYGYGYGYGYGYGYYVQ
                     DKKKGFFKRLFSGFKKD"
     gene            4095..4601
                     /locus_tag="PLU36_00205"
     CDS             4095..4601
                     /locus_tag="PLU36_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HMZ45200.1"
                     /translation="MLSKEKKWYVIYTKPRWEKKVDTQLTRKNIVSWCPIQKIERQWT
                     DRKKVIEDPLFKSYVFVYTNEEERVEILRTEGVLNFVKYLKEPAVIKDEEINLIKRFL
                     AETDAKISIISDEGFKPDTLVKVSFGVFMNNVGKVVRGSKKKVYVQLQSLGQLMVVEF
                     PSGYLTPL"
     gene            4611..5597
                     /locus_tag="PLU36_00210"
     CDS             4611..5597
                     /locus_tag="PLU36_00210"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HMZ45201.1"
                     /translation="MNILLIGLGSIGKRHLKNILSIGYKNVTVVSSKNSLPEPFTALP
                     VFKTIADATQNIQFTTAVICVPTAQHRAALLECLQAGIEHIYLEKPVSNNFLGIEQIL
                     EVYSNANIYVGFDMHYDIGLQKVKQLLDENKIGKIVSANAQVGQYLPDWRPQEDYTKG
                     MSAKIDTGGGVMLDLVHEFDYLYWLFGEVDKIACFNKNSNSLNIETEDIAEVLLQFKS
                     GVIGTIHLDYLQQKLVRNCLITGSKGTIQWDLTSSKVNWITADKIENEYCYQGFERND
                     RFVNIMKDFLLQQRNSYTTNFTQGLESLKMVLAAKYSSQNNVFVKLDEFHPS"
     gene            5606..6301
                     /locus_tag="PLU36_00215"
     CDS             5606..6301
                     /locus_tag="PLU36_00215"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HMZ45202.1"
                     /translation="MFILGTICCRGGSKGVPGKNIKLLNGKPLIAYTIETALQTSLIN
                     DVIISTDSIDIANAAKLYGAKVPFMRPNDLATDAASKWPVFIHALEAYEKMFNVEVDY
                     LVDMDVTVPLKNSTDINGAIQKALDDSTVDVVITGYEPERNPYFNMMEILPNGFAEIV
                     KKGDKPIVRRQDAPEVYSLTPAAYVVKKSALYNYEHWSKAKCKIYPIPRERAVDIDTE
                     IDFKIVEFLMNKK"
     gene            6298..7095
                     /locus_tag="PLU36_00220"
     CDS             6298..7095
                     /locus_tag="PLU36_00220"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ45203.1"
                     /translation="MSINLFDLSGRVAIITGGAGLLASEHAIALSNYGAKVVLADFNL
                     EKCKSAVDILQKQSINAVAKFCDVTKKESWEALLAEVLKEFGSVDILINNAGFTNQSK
                     SANFDASFENFPLEDWNAIMNVNLTGTFLGCQTVGKQMLAQGKGSIINIASLYGVVSP
                     NHNIYPGTGISQPVAYSVSKHGVVALTKYVATLWASKGVRVNALTPGGIFNNHQGLFL
                     ERFKQLNPIGRMSEKEELRGGIVYLASDASSHVVGHNLIIDGGWTAW"
     gene            7266..7736
                     /locus_tag="PLU36_00225"
     CDS             7266..7736
                     /locus_tag="PLU36_00225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HMZ45204.1"
                     /translation="MTNKLTASTNDYGVLNIGKSGCLQLENHVKFSSGCRVYVNGLLT
                     IGENTYINPNSVIIVAKEISIGSNCAISWNVQIMDTDIHTLVVNGQPKESTKKIKIGN
                     NVWIGSNVIILKGAEIGNNVVIAAGSIVSGNIPDNVLIAGVPAKIVKEQISWLL"
     gene            8021..9067
                     /locus_tag="PLU36_00230"
     CDS             8021..9067
                     /locus_tag="PLU36_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018401706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HMZ45205.1"
                     /translation="MNNPFFEIAGRKIGYDYEPLVIAEIGINHEGSLQTAKEMVDAAK
                     AAGIEIVKHQTHIVEDEMSSAAKNTIPGNATVSIYEIMKRCSLNEEDELEMKNYVESK
                     GMIFISTPFSRAAADRLHKWNIPAYKIGSGECNNYPLLEHIASFGKPIILSTGMNTIE
                     SIAKAVAIFKKHHVPYALLHTTNLYPTPNHLVRLGAMTEIQKNFPDAVVGLSDHTLTN
                     HACLGAVALGASILERHFTDTMDRPGPDIICSMDINAAKELIEGSKIIALQRGGEKGP
                     AAEEQVTIDFAFATICTIKPIKAGEVFTKENIWVKRPGKGGILAEEYNNILGKKALVN
                     IDNDMQLQYSFIEK"
     gene            9071..10501
                     /locus_tag="PLU36_00235"
     CDS             9071..10501
                     /locus_tag="PLU36_00235"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HMZ45206.1"
                     /translation="MLFNSWVFVLFWIVFFFLYWFVFNKSIKLQNAFIAIASFVFYGW
                     WDWRFLLLLWLSLIVDFCVGYFLEIIENKSKRKLLIVLSIAFNIGLLMFFKYFNFFSN
                     SFESLAHSFGWQVDFVTLNLILPVGISFYTFQSLSYTIEVYNKKLKGTKDIFAYAAFI
                     SFFPQLVAGPIERAANMLPQYYKKRVFNYTQSVKGMQLILWGLFKKVVVADTCAIFAN
                     NAFNNYTQHDGFNLIMGAVFFSFQIYGDFSGYTDIARGLGKILGFELKINFMYPYFSR
                     SIAEFWRRWHISLSSWFRDYVYIPLGGSRSSKAITIRNTTVVFLVSGFWHGANWTFIV
                     WGALHAIYYVPLVIINKHKSFADTVAFNRTLPSFKELSFMIGTFILALIAWVFFRADS
                     IEHAFAFLKGMTNFKGFHNFGLIRCLPFISIMLLVDWVGRNNECALSDFAAKFPKWFR
                     YVIYLFVAVCIYANFSGEQEFIYFRF"
     gene            10505..11461
                     /locus_tag="PLU36_00240"
     CDS             10505..11461
                     /locus_tag="PLU36_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45207.1"
                     /translation="MQIFIKKIIIFIGLFLGISTLVALGTGQLALSKLSRKGRGVVID
                     KNNRLDSITKNKIVLIGGSNVCYGINSQMIEDSFNMPVVDMAINAGMGMSFYYQQVKH
                     AIKAGDIIIGIPEYYPYDGKITNGDAGIYSLAIIDYRNIKFFNAMQWLQLPIYLGDII
                     NENVKAFFTYEKNKITFGRFQYNKWGDYIGHKNEGSYADTFKHNKQYDLSVYENKNEI
                     YPDKNFINLIKNFEQFCNSKNIIYLHAYPVYSRYFYNDNVAKQIAEKIQPLKFINNPE
                     TYLYGFDSLYDTPNHILYKFRDERTSKLINDIRVALHQIKHE"
     gene            11454..12569
                     /gene="neuC"
                     /locus_tag="PLU36_00245"
     CDS             11454..12569
                     /gene="neuC"
                     /locus_tag="PLU36_00245"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: protein motif:HMM:TIGR03568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HMZ45208.1"
                     /translation="MNKKIVFLTGTRADFGKLKSLIEISNKNFEVHLFATGMHMDKKY
                     GYTVKEIEKCGYKNIFKFINHDASSMMDITLSRTIEGFANYIHLVQPDLIIIHGDRIE
                     ALAGATVGALNNILVAHIEGGEVSGTVDELIRHAVSKLSHIHLVANDEAKKRLIQMGE
                     NAESIYIIGSPDMDAMLSEDLPTIDEVKNNYEINFEKYAVSMFHPVTTEYDTMDEYAN
                     EYIKALEESNLNYIIIYPNNDKGSEFILNKIKRVEQNNKFRIFPSVRFEAFLVLLKNA
                     QFIIGNSSAGIREAPYYGIPTVNVGTRQNGRTSNPDIINTTYNSTSIINAIQEAKVKK
                     VTPRHLFGDGKSDEKFFQIISSQELWNTPKQKIFSDF"
     gene            12583..13431
                     /locus_tag="PLU36_00250"
     CDS             12583..13431
                     /locus_tag="PLU36_00250"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ45209.1"
                     /translation="MSDSRIVTVVDAKNKTFFFNFKELIRYKDLFYYMVLRDVTVMYK
                     QTVLGFLWAILNPFFQVVVFSVVFGTIAGIKTGIAGLPYPVFSCLAIIPWNYFSSTFS
                     ASSNSLVNTAGVFTKVYFPRLIIPMVPIFSKLVDFFISLIILVLVMGYYQYTPGSNWY
                     LFLLPLLIIILSSAGLGIWLSSLVLQYRDFRFAVNFLLPLLLYAAPVAFPALKVKEKL
                     GNTVYELYALYPMVGGIEGFRSSFVQNADFPWTLVLISGASALFIFISGAFYFKKMEK
                     HFADVA"
     gene            13421..14707
                     /locus_tag="PLU36_00255"
     CDS             13421..14707
                     /locus_tag="PLU36_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ45210.1"
                     /translation="MLHNDNNIAVKVTDLGKAYRIGVVEEKEENLSAVILKAILSPVK
                     NFRKIKSLKNISSEATGKDVFWANRNISFEVKKGEVLGIIGKNGAGKSTLLKMLSRIT
                     EPTEGRIELVGRVASLLEVGTGFNPELTGRENIFLNGTILGLTKKEIEERYESIVEFS
                     GIEKFIETPVKRYSSGMKVRLAFAVAAHLDPEILIIDEVLAVGDAEFQKKCLGKMQDV
                     AGKEGRTVLFVSHDMAAVKNLCNRAILLQHGSIVFEGSANDVVNYYLQNAASITSQSN
                     LDFSQAKGNKNFVVTDIEMLDENNNNIQIAESGRDFQLKISYETKEVAINPVVIILVK
                     NNLEQVLFTCLSRNSYNGTMQLNSQGSIICNIPKLPLLSGLYTLDIVLKYDQEVTFSL
                     ENIFSFEVEKGDFFGTGKINDDMMNGMIINHNWVIQ"
     gene            14710..16101
                     /locus_tag="PLU36_00260"
     CDS             14710..16101
                     /locus_tag="PLU36_00260"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HMZ45211.1"
                     /translation="MQKKRLLITISFSFSIRYIVRTGLLEKIKAFAEPVIVLTWNEED
                     LIKELRNKGYEVYILPELIRGTAYKDIRKKIDYWFVHFRLKSPTKKNQDKFLKQYNSF
                     KQNFIAQSRKLYNIAKMYLPGVQTKMFEKEKKLLISDTNYHQILKWVKPLNINAVFTV
                     TPFHTQEDILLRVCNDLGMKMITSILSFDNITKRGWIPVNYNVYMVWNKYNFNELVRI
                     YKGINKDKIFITGAPQFDFYYNPNFLLTKEEWMKTVGIKDATKKIILYAGGPKSLFPN
                     EPQYLQHINDAINNNEIEGSPIVLFRCHPVDILQRWKDSVGSSNNIVFDSSWTGEKNL
                     VSTNITEFDIKKLCSTLYYTDVHINLCSTMTVDGSAFNKPQIGPAYDDIAPAKAHLLK
                     GMYWQEHFIPVLKVNGIALANSKQELIELTNKALKHSDMDEQKCKQVVEEIITYANGN
                     STQRVAAILENEI"
     gene            16146..17402
                     /locus_tag="PLU36_00265"
     CDS             16146..17402
                     /locus_tag="PLU36_00265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMZ45212.1"
                     /translation="MKITHVLFGYHPSVGGTQIFYKGISENCVAWYKDEVEVLTTNSY
                     YGPHTNSFREISIPEETINGVLVKRYPFRRNHKKIIGFLNKLVAKITNKQSNYFIEML
                     TGPLSKQLFKALNSTNADVISASSLGYKYMQYPLYRHTLKTPKPFVFQGAIHFKSDEH
                     DGGITPKMLQAIKASEYYLCNTAYEKNRLTALGVNQENIVITGCPVNITQYENAKGHE
                     VRKQLNLSSNDVLIGYIGRLEATKSIPVLINAFEAAFQKNNQLHLVIAGFETAYLKQL
                     KQIVAKLDANIQCKISFLISISENEKANLFQAIDVLVLPSTNESFGIVFLEAWACKKP
                     VIGTAIGAVSSVISNGTDGLLMQPYNTIDLTEKILQLSNDKNLREFMGANGYNKVIQN
                     YTWEIVTKKFRDTYVAAIEKFKKNNF"
     gene            17423..18037
                     /locus_tag="PLU36_00270"
     CDS             17423..18037
                     /locus_tag="PLU36_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45213.1"
                     /translation="MFREEAIWINNALAKIKPLQDNNLVANLGSSTAYFREVIQPHIQ
                     QYLMQPLANNGWNIINIDLKQEDGVDVVADVTKIDFSQKIQPAGLTICTNMLEHVEDI
                     NLVVKNLIKVTTKGGYILLTVPYKYKKHLDPIDNMFRPTPNEIISLFKDVATEVIDKQ
                     VIVIHDKSYYHKKKSKFPIWGNREIIAYHLGIRHKVSGVLLQLK"
     gene            18047..19894
                     /gene="asnB"
                     /locus_tag="PLU36_00275"
     CDS             18047..19894
                     /gene="asnB"
                     /locus_tag="PLU36_00275"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HMZ45214.1"
                     /translation="MCGIVGVFSYKCRTEKYNTYLSKCCKSMKHRGPDDSGLWYNNEN
                     YSAAFVRLSIRDLSLNGHQPMISACGNFVITFNGEIYNSNNYVAELKKLGVHFKSNSD
                     TEVLLYALIYFGIEKVLAEFDGIFAFSFYDVQKNKLFIARDRVGTKPLYIGFNNDFII
                     YSSQYDHIINIDFIKNNSLDYQSLGNYLSFGYILAGEGVIKKTFSLPQGHYLETTNDS
                     FLIHKYYEFDEKKNISISNVEALFEEATISQMVSDVPLGTFLSGGVDSPLINFWANKK
                     QQVKAFTIGNETDELDESYYAKAFANKIGVEHHFKKITENDFLQLLEDNFKAFSEPFA
                     DFSSIPTMLVSKMAKEHLTVILSGDGPDELFWGYNRNVIFPNKATLFNQGKLSLAVNK
                     ILGKGNISKRYFTADSIAHFYLQSLQIHGAEASLPKVYLEKIVNNKIFSLLPERLQQP
                     KNMNDIMQMVRWLEMNIHLQRILLKVDRASMFYSLEARVPYLSNAVLDYASTLNYSDC
                     INENQGKVNMKRILETKVDKEWVYKKKKGFDIPMRQWIQNEIKQDVYETILNMPTELA
                     CVFEIDNLENILQNEYDASNKNSNYGFIWAVYSLVKWHQNHRNSSILKD"
     gene            19900..21075
                     /locus_tag="PLU36_00280"
     CDS             19900..21075
                     /locus_tag="PLU36_00280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMZ45215.1"
                     /translation="MNIAIVVNNFPSNSETFIINKVIELAKAKNSVTVFSLNGTGNKD
                     LYELYHFKNLPNISIKYINLPKNIFELIKFFLSNPLLTISSFSATPKIFFSNIKSTLL
                     TKHFNNKFDIVHFEYSGLGASLLEIIPLVKSKKVVSCRGSAEKVKPLTDATRKEKLQQ
                     LFNLVDGIHCVSEDMKQTITPYCNNIEKVFVNRPAIDATFFQPPAVKPQNNITQILTV
                     GRFTFQKGYLLLLMVAKKLATKNINFVWNIIGEGPQHEELVFHIHTLGLEKCVKLVGK
                     KNKNEVNQYLASANMFVLTSVYEGIPNVVLEAMAMELPVISTNCGGVEEVIETGIDGF
                     IAPLYDVETIANQIEYLIAKPDKANEMGRLARKKILESFTLERQVNIFVEAYNKIIG"
     gene            21085..22434
                     /locus_tag="PLU36_00285"
     CDS             21085..22434
                     /locus_tag="PLU36_00285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HMZ45216.1"
                     /translation="MIFSFIKYTQPAWYFNVLPSVKEFSCCYYHPKWLPLPNGILLDE
                     DTAYETEAAINADIGYRAWHSGYLIKACNNDLQKIIQLPKPSINDEYRFVTKYWGMHW
                     AALTLVRRIFTLHNPIKEIKGFIKNVNTPRYNLYNTVADWSEYKNFQSVLVEQQPLVS
                     VIIPTLNRYEYLADVMLDLEKQTWKNFEVIVIDQSENFNDAFYKKYNLKINVVQQKEK
                     LLWTARNNAVKLSKANYLLFFDDDSRVEPDWIEQHIKTLDFFNASISAGVSIAVVGGK
                     VSKSYDHFRWADQFDSGNALVKREVFEKIGLFDLQFNKERMGDSEFGIRAYLNGYKSI
                     SNPYGKRVHLKVSSGGLREIGHWDAFRPKKLFAPKPVPSVIYLFKKYYPSYLSKNVIY
                     LGIMLSNISYKSKSKNSMLALSVLLTFLKFPLLYIQYKKSLALANKKLQQGDKIEKL"
     gene            22457..23620
                     /locus_tag="PLU36_00290"
     CDS             22457..23620
                     /locus_tag="PLU36_00290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ45217.1"
                     /translation="MNLGIVSDCVHFTTPTGVVATDNHIFLRQLNALAVHFDRVTICC
                     PFIEFDDSKSYSVYTDKKFHFIPLSNPGGNSIKHKLKLLLTIPEWFKAFSKINKASDI
                     VYQRFPNNLNIPGFFYFYFKRKKVFATYTGAWKNEINQSKTYQFQKWLLKKFFRGPVW
                     VYSSEEKLAKNIFGGFSPSYTIQEWNEETVQVNNRINFLSNNKLTSLKLISVGALNSN
                     KNQQYILNNCLLLKQATIPFSLHIVGEGVLKNEYQNFIDQHNLNNEIILTGALNYTAL
                     RNLYREKDFVVQATLSEGFGKVPIEGFFHGLVPILHQSVMAPYFTNNGTRGYLFNAEN
                     SNDLFHVLSNVYSTTSAKLIAEKIKEGRIFVQSQTLENWASIYFSTVKKYFDL"
     gene            23610..24944
                     /locus_tag="PLU36_00295"
     CDS             23610..24944
                     /locus_tag="PLU36_00295"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HMZ45218.1"
                     /translation="MIYKAIIWKFLLALGIGVIIGAKLINVQLFGFAYFGAVLLLAYF
                     AVFNKIEQLFTVLPFVIYSEIFVRDRLDSLVPYLTIQYLYIFIFSFMLIRFSGGNKPH
                     NKAFYFLLGFAFLEIFNGLNPDRPKLLRSIFFNSFSMILAVIWASHNVLTPKLINKIL
                     ANVKLAGMYLSGIVIVAQVTGGIDYGNFSNSESSNGLAPVQLSGYLSVAACFIFFSLM
                     NKDEIKSRYVYIIAFSIITGVMILTFSRGGLYFLGAILTLYLFFNRKNLGNYFKYLLF
                     LPIGLVIYNLAIEKTEGKIIARYEAKGTSNRDILVEAAFKLFLENPVVGVGTSNYGTA
                     IVEYRLFNQESTAHNEFARAMAEHGILGIITYWGFFISLFITIFKRKEPAKQFSVYFI
                     ALFIFITIHNGLKISIQHLLIIMAIANPTAVKAIFYRKIKPNTIVKQSAISY"
     gene            24916..25569
                     /locus_tag="PLU36_00300"
     CDS             24916..25569
                     /locus_tag="PLU36_00300"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ45219.1"
                     /translation="MSNNQLYHTNSSHLPQLALCHQKCFKGSLSEKLGLAYIAKTLDW
                     FLKDDNKFLFHFQNTEGQVIGYCGGFVSKKNGDGSSSGMLQHAFKEAVIGIVKKPWLI
                     FNKEVVAMYPFIFKNIKQKLFKKSKAKQVVDYSNEEKKFAGLVVIGVHPAYRGKGFFE
                     KLMEQFFIDASLKGAIGGALSVKKNNARGIAAYKKNGWIIVREDAHTYIMQKLTQTS"
     gene            25569..26615
                     /locus_tag="PLU36_00305"
     CDS             25569..26615
                     /locus_tag="PLU36_00305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMZ45220.1"
                     /translation="MQQSILLIGLFLSEKNKHKIYRTAADQLAELLHKNNYSIIKAST
                     YVNKIPRFVHTIFSIIFNSSKYSVAIVPLYGGTFSLFWAEAATVFLKLFRKKIILIIH
                     GGSIPTRMENDRKAKNYLRTIQKAHVVVCPSNYIIHHLQKHGVNAVLIENVLNLSEYS
                     FHEKKLLRPTLFWMRTFEDVYNPLMAVRVLAILQNTYPSAKMIMAGRDAGLLEETKQL
                     AQKLNVIDKIVFPGYIDNTAKNNIAQEYDIYICTNSIDNAPVSTIEMMSLGLPVVSTN
                     VGGIPFIVTNNKDGLLVNLNDDEGMVSKIALLIENINIAEKIIQEGLNTSKKYDEEIV
                     IKKWNKIFKQLNFT"
     gene            26615..27631
                     /locus_tag="PLU36_00310"
     CDS             26615..27631
                     /locus_tag="PLU36_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HMZ45221.1"
                     /translation="MDKIKFALIGCGRIAQRHAEHINKLGILKAVCDTVEEKAKALAE
                     KYNVPYYTNFEEMLLKEKEVDVVSICTPNGLHATHAIAALNANFHTLVEKPMALSVYD
                     CGEMIKAAEKANKRLFAIKQNRFNPPVVAVKEAIDNNILGKIYNIQLSCFWNRNKDYY
                     ENSWKGTHDLDGGTLYTQFSHFIDLLYWMIGDVKRAYALTSNFSHQGIIDFEDTGVIA
                     LEFYNGAIGTINYTVNSYQKNMEGSLTIFAEKGTVKIGGQYLNELEYQAIENFEIKDL
                     PTGNTANNYGTYVGSMSNHDKVYENLIDVLQNGKSIATNSFEGLKTVEIISKIYQSAK
                     ELNK"
     gene            27659..28765
                     /locus_tag="PLU36_00315"
     CDS             27659..28765
                     /locus_tag="PLU36_00315"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HMZ45222.1"
                     /translation="MNIPFVDLKQQYQSIKNEIDTAIENVLTETAFIGGKYVNKFEKD
                     FETLYGVNHCISCANGTDSLYIIMKMLGIGAGDEVITVANSWISSSETISQTGAKPVF
                     IDIEEEYLSINEKLIEQKITSKTKALIMVHLHGQMCDIETIAQLCKKYQVYLIEDCAQ
                     SHLSEYNNIRAGLRGIAASFSFYPGKNLGAYGDAGCIITNDSALAEKCKMYARHGALI
                     KHQHKIEGINSRLDGLQAAVLSAKLPFLIEWTNKRIAAAKHYDEALKNINEIVTPKVR
                     ANSKHTFHLYVIRAKQRNELANYLKEKGIETAIHYPTPLPFLEAYNYLNYTELDFPVA
                     NKFKNEILSLPIYPEITIEQINYIALTIKSFYSK"
     gene            28762..29754
                     /locus_tag="PLU36_00320"
     CDS             28762..29754
                     /locus_tag="PLU36_00320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ45223.1"
                     /translation="MIISIISPVFNEEKYIHQTIKSFLEQEHTSFDVEILLCDGMSTD
                     KTRDIIKSYAEQYANIRLIDNPKRKTPFAFNIGIKEARGEYVAILGAHSAYDKNYLQA
                     CYNELIATNSIACSGRVITQSAFDTLEAKLCEWAMLSQFGVSGNSFRLMKEGYTHSVN
                     FPVVKKEALLSLGGYNEMLERNQDNDMNQRLIDAGYQLYCTWKTKCFYRPPATLNKLF
                     KYGYRGGFWNAKSFFAHKKSMRIHHFIPFFFVVALLTTFTLGVVEFVLGKSSYFFSLF
                     SLIVFFHLLAGLFFSAISLKYESDSRKIFLPFVFFLFHFSYGWGTVKGFVDYKK"
     gene            complement(29781..32102)
                     /locus_tag="PLU36_00325"
     CDS             complement(29781..32102)
                     /locus_tag="PLU36_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HMZ45224.1"
                     /translation="MNHIPQLITDLGLILGAAAIVTILFKWLKQPLVLGYLIAGFLVG
                     PNLHLTPSIAEVANIKVWAEIGVIFLLFGLGLEFSFKKLMKVGAPASITAIIEVSGMV
                     FLGFMLGKLMGWKQIDSIFLGGVMSISSTTIIMRAFDELGVKGKQFAGLVFGILVVED
                     LIAIVLLVLLTTLSISSTFSGSEMLFSVVKLIFFLALWFVLGIFFVPTILRKAKRLLN
                     DEMLLITSLALCILMVWLAARVGFSPALGAFIMGSILAETVYAEKIEHLLKPVKDLFG
                     AVFFVSVGMLFNPQIVVDYAGIIILMIVVIISGKFFFASLGALLSGQALKTSVQAGFS
                     LTQIGEFSFIIASLGLSLNVTSDFIYPIAIAVSAITTFTTPYLLKLSDPFYNWIVKIL
                     PVNWVKAINRYSNRSQKQSIKNEWQEYVRSWIISSFLLTVLISFVVLLSSYYILPYTR
                     TKLGDNTWATIVGSILALIVMAPFLWNLVIPFKKDKLKELAQIQRFRGSLYVLHFIRI
                     AIAIFLIGFLLDRFFSPKIALTVSIIMFIILIIFSKKIQALYNKLKNQFLLNYNEREA
                     IKITEVQSELAPWDAHIVNFTVDANFNGIGQTLLELGWREDFGINIARIFRGEKIINT
                     PGRYERIFPSDKLSIIGTDEQLMQFSSFMKAEVVNKKESTTQVKRNEITLEQLIIIPN
                     SLFVNKTIRESKIREQTHGIVVGVETNGERIVNPESVYTIKTGDILWIVGDKLRIAAI
                     KKAEEELATILNNNAKEGEDFSNYSSEESYTNT"
BASE COUNT        11861 a         4182 c         5355 g        10766 t
ORIGIN      
        1 gttgtaccag atattgtgat gccagatact tacgagtatt tagaatttag agaaaaagac
       61 aatagtaact cattgccatg ggatgaaatt ccaaaagcag attatgcagt agatagtaat
      121 tccaatgaat tgaattttat tattagttca gaaaataaaa tgattagtga aaacaataac
      181 ttaaatatta ttcataataa tgcactttgg cttgccaata atactaaaaa acctatttca
      241 ttaaactttg ataattataa aatatttcaa aagcaaatta aaagcacagt agcacaaaca
      301 acaacattat ctaaattatc taaagaaatg gatgtaactg taactagtgc agataaatca
      361 aaatattttt ctaatgttga taaggcaaaa ggagatcgtt atcaggcttg gttaaaaaat
      421 ataaaaaatg atatgtatat ttcaacaaca gcagatatga ttgtaaatat ggcaaacaaa
      481 aaaattgtat ataactacta atttattttt ttaaatcttc ttttaagcaa ctctttcagg
      541 gttgcttttt atttatatcc acattgtgtg gatttttact ctacttcatc tgagttgcta
      601 aaagttaact ttgaaatgtt actttaaccc attgatttat atgccaaaat ccattacaag
      661 tagttatgtg aaaataacac gcataattgc attagtaatt gctgtaagta tgtttaccac
      721 atcttgcacc aattctaaaa aactggtaat acttaatgat atacctgata cagcaagagt
      781 ggctttgcca ttcattgaaa cacctgtaaa tacaatacaa ccagatgata ttttagaaat
      841 aaaaatttca ggtaaaaacc ccgaaacatc caacgacttt aactctaaag gaggaggata
      901 ttatttaaac actggtgcta gctctgcggc agttcctaat tatttagtag ataaaaatgg
      961 atttatagaa atttataaat taggcaaaat aaaagcagca ggtttaacaa aagatcaatt
     1021 aaaagaaaat ttacaatctg ccttagaaaa agaattggtg gaaccaactg ttgttgtacg
     1081 ctttgtaaat atgcgtttta ctgttttagg agaagtccgt acaccatcta cttacactat
     1141 acctaacgaa aagttaacta tactagaagc agttggctat gcaggagatt taactatgta
     1201 tgctaaaaga aattctataa gagtaataag agacagtagt ggtcatagag aaataggtag
     1261 cataaattta aatcaaaaat cattatttac atctcctttt tactatatca aaagaaatga
     1321 tgtgatattg atagagagtg attatacctc taaaaaacaa actgaagttt ttgcgaaaac
     1381 tgccagtata ataggtattt tatcgggttt aataactgta gtgtatttaa tattaagaaa
     1441 gtaaaataat taatgagtta cccaacaaaa ggttatgaac aagaggttgt gcagcaacaa
     1501 gcaagcaaca accaattaga tgctaaaaag cttatttata agcttattgg cattttgcct
     1561 tgggtaatta tttctctagc aataagcatt tttgctgcta acttatattt aagatacact
     1621 ccaaaactat ttagagtgag tggtaattta ttaataaaag atgaagacca gtcaagttat
     1681 agatcattaa aagatttagg cattattcca ggaaatattg atgtgcctaa ccaaatgcaa
     1741 attttaaact cttatacatt aacggaaaga gttatagatt ctttaaaact aaggctcaag
     1801 cttagacaag aaggtagggt tatttcttca gaattgtatg gcaaaacaat gccctttaaa
     1861 ataaatgttg tagaagagta cccaaacaca aaggcttctg cttaccacat taatctaaca
     1921 agagaaaatt ttattatttc aggtgcagag gggcaaaaga aatacacttt tggtgatacg
     1981 gttgatattt cttatggaaa aataatttta gaaagaaacc ctttagtaaa aataaacccc
     2041 aatggatacc aacttaccat caatgattta gaatctgaag taagggcttt aagatctaaa
     2101 attactgttg ttcaacttac tgaaaatggt ggtattcttg aaattgctac caatgaagag
     2161 ttgccagata gaggtattga cattattaat aaattgatgg aagtatatga taatgcagga
     2221 ttagatgata aaaatattgt tgctaaaaaa accattcaat tcttaaatga taaattagga
     2281 aacattgcaa aagatttaga tagtattgaa attaaaattg aacaatttag aaaagacaac
     2341 aaagtaccct cttttagcga agctggaagt gcattttttg cagaatatac agggcttgat
     2401 aagaacaaga aaatattagt tacacaaagt gcaatgttag atcatttaga atcttacatt
     2461 acaaatttta gaaataccaa tgatattatt ccacccacat ttggacaaac agatgttatt
     2521 ttattagggt taattgaaac acacaataca accgtattgg caaagcaaaa attagaatta
     2581 atcagtaagc ctaccgattc tcatttacaa aatatgattg aaagtattaa gggaattagg
     2641 caaaatattc taagagcaat cagaaatatt agagttgcta accaaacaca gttacagaat
     2701 tacgaaagca attacagcct attagaatct aaattgcaaa gcattccaga aaaacaaaga
     2761 attgaaatgc gattagagag ggatagagta gtaaaacaag aaatttataa agacctctta
     2821 aaaagaaaag aagaaaccga actctcacta gcttcaaaca ttaataatac acgtattgtt
     2881 gactatgcac aaaactatgg ccctaaaagc cccaatatag caactgcaag aagtgtagct
     2941 attttatttg gcatattaat tcctatatta ataatagtta tcaaagattt ctttaacact
     3001 aaaatacaag acagaaaaga aattgaacaa gccacagcaa taccaatttt aggagaactt
     3061 tcttatgaaa aagataagaa gaatgtagta attgaaaaca aaagcagaag cccaatagcc
     3121 gaacagttta gacttataag aactaatttg cagtatatgg tacccgataa gcctttacaa
     3181 agtttattgg taacttcatt tatgagtggc gaaggtaaaa gttttgtatc gttaaacttg
     3241 gcaagtagtt tagctataac aggtgccaaa acagttatat tagaatttga tttaagaaaa
     3301 ccaaaattaa gtaaatattt agaactctct aacgatatag gcctttctaa tttcattatt
     3361 aaggatatga atattgatga aattattcag gaagttccca atagagaaaa cttatttgtt
     3421 ataagttcag gccctattcc acccaaccct gcagagttat tattaagtca aagaacagta
     3481 aaattatttg aacaattagc tacaagattt gattacatta ttatagatac tgcaccagta
     3541 ggcttggtta cggatgcatt attactagaa aaatttgttg atttaagctt atttatttta
     3601 cgtcataaat attcttttaa agccgaaatt ccttttgttg ataaattata ccgagataag
     3661 aaatttaaaa acatgggtat tattgtgaat ggtattaaaa atgaaggaag tggttatggc
     3721 tatggnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnacatg ggtattattg
     3841 tgaatggtat taaaaatgaa ggaagtggtt atggctatgg ctatggctat ggctatggtt
     3901 atggttatgg ctatggctat ggctatggtt atggttatgg ttatggctat tatgttcaag
     3961 ataaaaagaa aggcttcttt aaaagattat ttagcggatt taaaaaagat taatgctttt
     4021 cgcaatttaa acattgttca ctctcttaat ttttaactgt gagtgaggtg gcggagctgt
     4081 aaaaagttat cagtatgctt agcaaagaaa aaaaatggta tgttatttat actaaaccaa
     4141 gatgggaaaa aaaagtagat acacaactca caagaaaaaa tattgtaagc tggtgtccaa
     4201 ttcaaaaaat tgaacgacaa tggacagata gaaagaaagt gattgaagat cctttattca
     4261 aatcctatgt ttttgtatat accaacgaag aagaaagagt tgaaatttta agaacagaag
     4321 gtgttttaaa ttttgtaaaa tatttaaaag aacctgcagt aataaaagat gaagaaatta
     4381 atttaataaa acgcttctta gcagaaacgg atgctaaaat atcaattatt tcagatgaag
     4441 gctttaagcc agatacatta gtaaaagtaa gctttggagt gtttatgaat aatgtaggaa
     4501 aagtagtaag aggtagcaag aaaaaagtat atgttcaatt acaaagttta gggcaattaa
     4561 tggttgtgga gtttccgtca ggttatctta caccgctata atatattata atgaatattt
     4621 tattaatagg attgggctca ataggtaaaa ggcatttaaa aaatatctta tctattggtt
     4681 ataaaaatgt aaccgttgta agtagtaaaa atagtttacc agagcctttt acagcgttac
     4741 ctgtttttaa aacaattgca gacgctaccc aaaatattca atttacaact gcagttattt
     4801 gtgtgccaac tgcacaacat agagctgcac ttttagaatg tttacaagct ggtattgagc
     4861 atatttattt agaaaaacca gttagtaata attttttagg aatagaacaa attttagaag
     4921 tttattccaa tgccaatatt tatgtaggct ttgatatgca ttacgatatt gggttacaaa
     4981 aagtaaaaca acttttagat gaaaataaaa ttggtaaaat tgtttctgca aatgcacaag
     5041 tagggcaata tttaccagat tggcgaccac aagaagatta caccaaaggc atgagtgcta
     5101 aaattgatac tggaggaggt gtaatgttag atttagtgca tgaatttgat tatttatact
     5161 ggctttttgg agaggtagat aaaattgcat gctttaataa aaactctaat tctttgaata
     5221 tagaaacaga agatattgca gaagtgttat tacaatttaa atctggggtt attggcacta
     5281 ttcatttaga ttatttacaa caaaaattag tacgtaattg tttaattact ggtagcaagg
     5341 gtacaataca atgggattta actagtagta aagtaaactg gataacagca gataaaatag
     5401 aaaatgagta ttgctatcaa ggctttgaac gtaatgatag atttgtaaat attatgaaag
     5461 attttctttt acagcaacgt aattcatata ctacaaattt cactcaaggt ttagaaagtt
     5521 taaaaatggt tttagctgca aaatattctt ctcaaaataa cgtttttgta aaactggatg
     5581 aatttcatcc ttcataattt ttttaatgtt tatattaggt acaatttgtt gtagaggtgg
     5641 cagtaaaggc gttccaggta aaaatataaa actattaaat ggcaaaccat taatagctta
     5701 tactattgaa acagctttac aaacttcgtt aattaatgat gtaattattt ctacagattc
     5761 tattgacatt gcaaatgcag caaagcttta tggagcaaag gttccgttta tgagacctaa
     5821 tgatttagca acagatgcag caagtaagtg gcctgttttt attcatgcat tagaagctta
     5881 tgaaaaaatg tttaatgtag aggtagatta tttggtagat atggatgtaa cagttccttt
     5941 aaaaaatagt actgatataa atggagcaat tcaaaaagca ttagacgatt ctacagttga
     6001 tgtagtaatt acaggttatg agcctgagcg aaatccatat tttaatatga tggaaatttt
     6061 acccaatgga tttgctgaaa ttgtaaaaaa aggagataaa cccattgttc gtaggcaaga
     6121 tgctccagaa gtatatagcc ttacaccggc tgcttatgta gtaaaaaaat ctgccttata
     6181 taattacgaa cattggagta aagcaaaatg caaaatttat ccaataccaa gagaaagggc
     6241 tgtagatatt gatacagaaa ttgattttaa aattgttgaa tttttaatga ataaaaaatg
     6301 agtattaatt tatttgattt atcaggtaga gtagccatta ttacaggtgg tgcagggctt
     6361 ttagcaagtg aacatgctat tgctttaagc aactatggtg ctaaagttgt tttagccgat
     6421 tttaatttgg agaaatgtaa atctgcagta gatattttac aaaaacaaag tattaatgct
     6481 gttgcaaaat tttgcgatgt tacaaaaaaa gaaagctggg aagccttatt agcagaggtt
     6541 ttgaaagaat ttggaagtgt agatatttta ataaataatg caggttttac caaccaaagc
     6601 aagagtgcca attttgatgc aagcttcgaa aattttcctt tagaagattg gaatgctatt
     6661 atgaatgtaa atcttacagg aacattttta ggttgtcaaa ctgttggcaa acaaatgttg
     6721 gcacaaggca aaggcagcat cataaatatt gcatcattgt atggtgtggt aagtcccaat
     6781 cataacattt atcctggtac aggtatttct caacctgtgg catatagtgt aagtaagcat
     6841 ggtgtagtag cacttacaaa atatgtagca accttgtggg caagtaaagg cgttcgtgta
     6901 aatgcactta ctccaggtgg catttttaat aatcatcaag gattgttttt agaacgcttt
     6961 aaacaattaa atcctattgg tagaatgagt gagaaagaag aattgcgtgg cggcattgtg
     7021 tatttagcaa gtgatgcaag cagccatgta gtaggacata atttaattat tgatggtgga
     7081 tggacagcat ggtaaaaatt aaaagacagt tgaggtggct aaaaaagtcg aatactaagt
     7141 gttatgattt ttttcaactt atttatgcct acttgttcag accaagaaac actttcattt
     7201 gctctaaagt taagtttact aataggggta aattgaaagt taatggtgat ttttattttg
     7261 gtgttttgac aaataaatta accgcctcta caaatgatta tggcgtatta aatataggaa
     7321 aaagcggatg tttacaatta gaaaatcacg ttaagttttc ttctggttgt agagtatatg
     7381 taaatggatt acttacgatt ggtgaaaaca catacattaa ccccaattca gttattatag
     7441 ttgcaaaaga aatttctatt ggcagcaatt gtgccatttc atggaatgtt caaattatgg
     7501 atactgatat tcatactttg gttgtaaatg gacaaccaaa ggagtcaact aaaaaaataa
     7561 aaataggaaa caatgtttgg attggctcaa atgtaatcat attaaaaggt gcagaaattg
     7621 gtaataatgt tgtaatcgct gcaggctcta ttgtaagtgg caacatacca gacaatgttt
     7681 taattgcagg tgtaccagct aaaatagtta aagagcaaat tagttggctg ctataaaaat
     7741 ttgaattatg aaaatggtta gttcgttttt gtttagtgaa gtttttgaaa agaaaatttt
     7801 attgagtcaa attatcctaa atatgtaaga gttaattggg caaaaataaa aataaacaaa
     7861 taatacagaa gttgattttc taaaaatcta aaaatagatt acctacaact attcattatt
     7921 aaatatcaaa taaatgatag ggtttaaaaa aataataaaa aagtacaagt ctatttttga
     7981 caattacaac attaaattaa aaaattagaa aagtatataa atgaataatc ccttttttga
     8041 aatagcaggc agaaaaattg gttatgatta tgaaccattg gtgattgcag aaattggcat
     8101 taaccacgaa ggctctttac aaacagctaa agaaatggtt gatgcagcta aagcagcagg
     8161 aattgaaatt gtaaaacatc aaacacatat tgtagaagat gaaatgagca gtgctgcaaa
     8221 aaacaccatt cctggcaatg ctactgtaag catttatgaa ataatgaaac gttgcagcct
     8281 caacgaagaa gatgaattag aaatgaaaaa ttatgtagaa agcaaaggca tgatttttat
     8341 ttctacacct tttagtcgtg ctgctgcaga tagattacac aaatggaata ttcctgctta
     8401 taaaattggt agtggcgagt gtaataatta tcctttgtta gaacatattg catcatttgg
     8461 taaacccatt attttaagta caggaatgaa tactattgaa agtattgcaa aagctgtagc
     8521 catttttaaa aaacaccatg tgccttatgc tttattgcat acaaccaatt tatatcctac
     8581 acccaaccat ttagtacgtt taggagctat gactgaaatt cagaaaaatt ttcctgatgc
     8641 agtagtaggt ttaagcgatc atacattaac caatcatgct tgtttgggag cagttgcatt
     8701 gggtgcaagt attttagaaa ggcattttac agatacaatg gatagacctg gtccagatat
     8761 tatttgcagc atggacatta atgcagctaa agaactaata gaaggcagta aaataattgc
     8821 tttacaacgt ggaggagaaa aaggtcctgc tgctgaagaa caagttacta ttgattttgc
     8881 ttttgcaaca atttgcacca ttaaacctat aaaagcagga gaggttttta ccaaagaaaa
     8941 tatttgggta aaacgccctg gtaaaggggg cattttagct gaagaatata ataatatttt
     9001 aggcaaaaaa gcgttagtaa atattgataa tgatatgcag ttacaatatt cattcataga
     9061 aaaataatgt atgcttttta attcttgggt ttttgtatta ttctggatag tttttttctt
     9121 tctgtattgg tttgtattta ataaatctat taaactacaa aatgccttta ttgcaatagc
     9181 aagttttgta ttttatggtt ggtgggattg gcgtttcttg ttattacttt ggctaagttt
     9241 aatagtagat ttttgcgtag gttattttct tgaaataata gaaaataaaa gcaaacgaaa
     9301 attattaatt gtattaagta ttgcctttaa tataggctta cttatgttct ttaaatactt
     9361 taatttcttt tcaaattcat ttgaaagttt agctcattca tttggttggc aagtagattt
     9421 tgtaacgcta aatttaattt tacctgtagg catatctttt tatacttttc aatctttaag
     9481 ctataccatt gaagtttata ataaaaaatt aaaaggcaca aaagatattt ttgcttatgc
     9541 tgcattcata agtttctttc ctcaattagt agcaggtcca atagaacgag cagcaaatat
     9601 gttgcctcaa tattataaga agcgtgtgtt taattacaca caaagtgtaa aaggaatgca
     9661 gttgatttta tggggtttat ttaaaaaagt tgtagttgca gatacctgtg ccatttttgc
     9721 taataatgcg tttaataatt atacccaaca tgatggattt aatttaataa tgggagctgt
     9781 atttttttca tttcaaattt atggcgattt tagtggttat acagatatag caagaggctt
     9841 aggtaaaatt ttaggttttg agttgaaaat aaatttcatg tacccctatt tttcaagaag
     9901 catagcagag ttttggagaa gatggcatat ttcactttca tcatggttta gagattatgt
     9961 ttatattcct ttaggtggct caagatcttc taaagctatt acaatacgaa atacaactgt
    10021 ggttttttta gttagtggat tttggcatgg tgctaattgg acatttattg tttggggggc
    10081 tttacatgca atttattatg ttcctttggt aattattaat aaacataaat catttgccga
    10141 tacagttgct tttaatagaa ctttaccatc tttcaaagag ctatcattta tgataggtac
    10201 ttttatttta gctttaattg cgtgggtttt ctttagagca gattcaatag aacatgcttt
    10261 tgcattttta aaaggcatga caaactttaa aggctttcat aattttggat taataagatg
    10321 tttgccattt atttcaataa tgcttttagt agattgggta ggtcgtaaca atgaatgtgc
    10381 tttatctgat tttgctgcaa aatttccaaa atggtttagg tatgtcattt atctatttgt
    10441 agctgtttgt atttatgcta atttttcggg agaacaagaa tttatttatt tcagatttta
    10501 gactatgcaa atttttataa aaaaaataat aatcttcatt gggctatttt taggcatttc
    10561 tactttagtt gcattaggta cagggcaact tgcgttaagc aaacttagta gaaaaggtag
    10621 aggggttgta atagataaaa ataacagatt agattctata actaaaaata aaatagtact
    10681 tattggtggt tctaatgttt gttatggcat caactctcaa atgattgaag actcattcaa
    10741 tatgcctgtt gtagatatgg ctattaatgc tggtatggga atgagtttct actatcaaca
    10801 agtaaaacat gctataaaag ctggtgatat tattattggc attccagagt attatcctta
    10861 cgatggaaaa attaccaatg gcgatgcagg aatttatagc cttgcaatta tagactaccg
    10921 aaacataaag ttttttaatg ctatgcaatg gcttcaatta cccatttatt taggtgatat
    10981 tataaacgag aatgtgaaag catttttcac ttacgaaaaa aataaaataa cttttggacg
    11041 ttttcaatac aataaatggg gcgattatat tggtcataaa aatgaaggtt cttatgcaga
    11101 tacttttaag cataataaac aatacgattt aagtgtttat gaaaataaaa atgaaattta
    11161 tccagataaa aattttatta acttaattaa aaattttgaa caattctgca attccaaaaa
    11221 tataatttac ctgcatgctt atcctgtgta ttcaaggtat ttttataatg ataatgttgc
    11281 aaaacaaata gcagaaaaaa tacaaccact aaagtttata aataatccag aaacttattt
    11341 gtatggtttt gatagccttt atgatacccc aaaccatatt ttatataaat ttcgagatga
    11401 aagaacatca aaactaatta atgatattag agtagctcta catcaaataa aacatgaata
    11461 aaaaaatagt ttttcttaca ggcacaagag cagattttgg caaactaaaa tcgttgatag
    11521 aaatttcaaa caaaaatttt gaagtgcatt tgtttgcaac aggtatgcac atggataaaa
    11581 aatatggcta cacggtaaaa gaaattgaaa aatgtgggta caaaaatatt ttcaaattta
    11641 tcaaccacga tgcatcttca atgatggata ttactttaag cagaaccatt gaaggttttg
    11701 ccaattatat tcatcttgtt caacccgatt taatcattat tcatggcgat agaattgaag
    11761 cattagctgg tgcaactgtt ggtgctttaa ataatatttt agtagcacac attgagggag
    11821 gagaagtaag tggtacagta gatgaattaa ttcgtcatgc tgtttctaaa ctaagccata
    11881 tacatttggt tgcaaacgat gaagcaaaaa aaagattaat tcaaatgggc gaaaacgctg
    11941 aaagcattta catcattggt tcgccagata tggacgcaat gctttctgaa gatttaccaa
    12001 ctatagatga ggtaaaaaat aattatgaaa ttaattttga aaaatatgct gtatctatgt
    12061 ttcatcctgt aacaacagag tatgatacta tggatgaata tgctaatgaa tacattaaag
    12121 cattggaaga aagtaatttg aattacataa ttatttaccc caacaatgat aaaggcagtg
    12181 agtttatttt aaacaaaata aaaagagtag agcaaaataa taaattcaga atttttcctt
    12241 ctgtaaggtt cgaagccttt ttagtgctgc taaaaaacgc ccaatttatt attggtaatt
    12301 caagtgcagg aataagagaa gctccttact atggcattcc aactgtaaat gttggtacaa
    12361 gacaaaatgg aagaacctct aatcctgata ttattaatac aacttacaac agcacatcaa
    12421 tcattaatgc tatacaggaa gcaaaagtaa aaaaagtaac acctcgtcat ttatttggtg
    12481 atggaaaaag tgatgaaaaa tttttccaga ttatttcaag tcaagaactt tggaatacac
    12541 ctaaacaaaa aatattttca gatttttaga taaatattca gcatgtcaga ttcaagaatt
    12601 gtaacagttg ttgatgcaaa aaataaaact ttctttttca actttaaaga gttgataaga
    12661 tacaaagatt tgttttatta tatggtgtta agagatgtta cagtaatgta caaacaaaca
    12721 gtattgggtt ttctttgggc aatcttaaat ccattttttc aggtagtagt ttttagtgta
    12781 gtatttggaa ctattgcagg tataaaaaca ggtattgctg ggcttcctta tcctgttttt
    12841 tcttgtttgg caattattcc atggaattat ttttcatcta cattttctgc ttcatctaat
    12901 agtttagtta atacagcagg tgtttttacc aaagtgtatt ttcccagatt aattattcct
    12961 atggtgccta ttttttctaa gctggtagat ttttttattt cactcattat tcttgttttg
    13021 gtaatgggtt attatcaata tacacctggc agtaattggt atttattttt acttccttta
    13081 ttaataatta tactttcttc tgcagggctt ggtatttggc tatcctcatt ggtattacaa
    13141 tatagagatt ttaggtttgc tgtaaatttt ttattgcctt tacttttata tgctgcacct
    13201 gttgcatttc ctgcattaaa agtaaaagaa aaattaggca acacagtgta tgagttatat
    13261 gctctttatc caatggtagg aggcatagaa ggctttagaa gcagctttgt acaaaatgct
    13321 gatttccctt ggacacttgt attaattagt ggagcatctg cattatttat ttttataagc
    13381 ggtgcatttt attttaaaaa aatggaaaaa cattttgccg atgttgcata atgataataa
    13441 catagcagtt aaagtaactg atttaggaaa agcttacaga ataggcgttg tagaagaaaa
    13501 agaagaaaac ttatctgctg taattttaaa agcaattctt tcgcctgtaa aaaattttag
    13561 aaaaattaaa tcactaaaaa atatttcttc cgaagcaaca ggtaaagatg ttttttgggc
    13621 aaatagaaat atatcttttg aagtaaaaaa aggagaggtt ttaggaatca ttggcaaaaa
    13681 cggggcaggc aaaagcactt tgctaaaaat gctttcaaga attactgaac ccacagaagg
    13741 aagaattgaa ttagtaggaa gagttgcaag tttattagaa gtaggtactg gttttaatcc
    13801 tgaattaaca ggcagagaaa atattttttt gaatggaact attttaggct taaccaaaaa
    13861 agaaattgaa gaaagatacg aaagcattgt agagttttca gggatagaaa aatttattga
    13921 aacacctgta aagcgttaca gtagtggtat gaaagttcgt ttagcttttg ctgttgctgc
    13981 acatttagat cctgaaattt taattattga tgaagtgtta gcagttggtg atgcagagtt
    14041 tcaaaaaaaa tgcttaggaa aaatgcaaga tgtggcaggt aaagaaggaa gaactgtatt
    14101 gtttgttagt catgatatgg ctgctgtgaa aaatttatgc aacagagcta ttttattaca
    14161 gcatggaagt attgtttttg aaggaagtgc taatgatgta gtaaattatt atttacaaaa
    14221 tgctgccagt attacatctc aaagcaattt agatttttcg caagccaaag gaaataaaaa
    14281 ttttgttgtt actgatattg aaatgttaga tgaaaataat aacaacattc aaatagcaga
    14341 aagtggtaga gattttcaac taaaaataag ttacgaaacc aaagaagtgg caataaatcc
    14401 tgttgtaatt attctggtaa aaaataattt agagcaggta ttgtttacat gcttatccag
    14461 aaattcgtac aatggtacaa tgcaattaaa tagtcaagga agcattattt gcaatattcc
    14521 taaactaccc ttattaagtg gtttatatac gttagatatt gttttaaagt acgatcaaga
    14581 agttactttt tctcttgaaa atattttttc ttttgaagta gaaaaaggag acttttttgg
    14641 aacaggaaaa ataaatgatg atatgatgaa tggaatgatt attaatcata actgggttat
    14701 tcaataagta tgcaaaaaaa aaggttactc attacaattt catttagttt ttcaataaga
    14761 tatattgtaa gaacaggatt gttagaaaaa ataaaggcgt ttgcagaacc tgtaattgtt
    14821 ttaacctgga atgaggaaga tttaattaag gagttaagaa ataaaggata cgaagtttat
    14881 attttacctg aattaataag aggcacagct tacaaggaca ttagaaaaaa aatagattat
    14941 tggtttgttc attttagatt aaaaagtcct accaaaaaaa atcaagataa atttttaaag
    15001 caatacaata gttttaaaca aaattttatt gcacaaagta gaaaactata caatattgct
    15061 aaaatgtatt tgccgggtgt gcaaactaaa atgtttgaaa aagaaaaaaa acttttaata
    15121 tccgatacaa attaccatca aattttaaaa tgggtaaaac ctttaaatat taatgctgtt
    15181 tttactgtaa ccccatttca tacacaagaa gatattttat taagagtttg caatgattta
    15241 ggaatgaaaa tgattacctc tattctttct tttgataata taaccaaacg tggttggata
    15301 cctgtaaact ataatgttta tatggtttgg aacaaatata attttaatga attggtgaga
    15361 atttataaag gaataaataa agataaaatt ttcattaccg gtgctccaca atttgatttt
    15421 tattataatc ctaatttttt actcactaaa gaagagtgga tgaaaactgt aggaataaaa
    15481 gatgctacta aaaaaataat tttatatgct ggtgggccta agtcattatt tcctaacgag
    15541 ccacagtatt tacaacatat aaatgatgca ataaataata atgagataga agggagccca
    15601 attgttttat ttagatgcca tccagtagat attcttcaaa gatggaaaga tagtgttggc
    15661 tcttcaaata atattgtttt tgattcatct tggacaggag aaaaaaattt agtaagtaca
    15721 aatattacag agtttgatat taaaaagctt tgctcaacac tgtattatac agatgtacat
    15781 attaatttat gtagtacaat gactgttgat ggaagtgcat ttaacaagcc acaaattggc
    15841 ccagcttacg atgatattgc acctgcaaaa gcacatttat taaaaggcat gtattggcaa
    15901 gagcatttta taccagtatt aaaagttaat ggtattgctt tggcaaactc aaaacaagaa
    15961 ctgattgagc ttaccaataa agcattaaag cattcagata tggatgaaca aaaatgtaaa
    16021 caagtggtag aagaaataat tacctatgca aatggaaatt ctacacaaag agtagctgct
    16081 attttagaaa atgaaattta aattgtgtat ttctcaaata aattaaaact tctttaataa
    16141 cattaatgaa aattacacat gttctttttg gctatcatcc atcagttggt ggtacacaaa
    16201 tattttataa aggtatttca gaaaattgtg tagcctggta taaggatgaa gtagaagttt
    16261 taactactaa ttcttattat ggcccacaca ccaacagttt cagagaaatt tctataccag
    16321 aggaaacaat aaacggagta ctagtaaagc gttatccttt tagaagaaat cataaaaaaa
    16381 ttataggttt tttaaataag ctagttgcaa aaattaccaa taagcaatca aactatttta
    16441 ttgaaatgct tacaggccct ctatccaaac aattatttaa agcattaaat tcaacaaatg
    16501 ccgatgttat ttcagcttct tcattaggtt ataagtatat gcaatatcca ctgtacaggc
    16561 atacacttaa aacccctaag ccatttgttt ttcagggagc aattcatttt aaaagtgatg
    16621 aacatgatgg tggcattaca ccaaaaatgc tgcaagcaat taaagcatct gaatattatt
    16681 tgtgcaatac agcgtatgaa aaaaacaggc taacagcgtt aggtgtaaac caagagaata
    16741 tagtaataac aggttgccca gttaatatta cccaatatga aaatgcaaaa gggcatgagg
    16801 ttagaaagca attgaactta tcatctaatg atgtattaat tggttacatt ggaagattag
    16861 aagcaacgaa aagtattcct gttttaataa atgcctttga agcagcattc caaaaaaata
    16921 atcagttgca tttagtgatt gcaggttttg agactgctta tcttaaacag ttaaagcaaa
    16981 ttgttgcaaa attagacgct aatattcaat gcaaaatttc gtttttaata agcatttctg
    17041 aaaatgaaaa agcaaatttg tttcaagcta ttgatgtatt ggtattgcca tctacaaatg
    17101 aatcttttgg aattgttttt ttagaagcat gggcttgtaa aaaacctgta attggcactg
    17161 caataggtgc tgtaagctct gtaatatcaa atggtacaga tggtttgtta atgcaaccat
    17221 ataacacaat agatttgact gaaaaaattt tacagctatc caacgataaa aatttaagag
    17281 aatttatggg agcaaatggc tacaataaag taatacaaaa ctatacatgg gaaattgtaa
    17341 ccaaaaaatt cagagatacc tatgttgctg caatagaaaa atttaaaaaa aataattttt
    17401 aattcatcaa aaattagaat gaatgtttag agaagaagcc atttggataa acaatgcttt
    17461 agccaaaatt aaaccactac aagataataa tttggttgct aatcttggtt catcaactgc
    17521 atattttaga gaagttatac agcctcatat tcagcaatat ttaatgcagc ctttagcaaa
    17581 taatggttgg aatattatta atattgattt aaagcaagaa gatggcgtag atgttgttgc
    17641 agatgtaaca aaaatagatt tttctcaaaa aattcaacct gctggtttaa ccatttgtac
    17701 aaatatgtta gagcatgttg aagatataaa tttggttgta aaaaatttaa taaaagtaac
    17761 tactaaaggt ggttatatat tacttactgt gccttataag tataaaaaac atttagatcc
    17821 tatagataat atgtttaggc caacaccaaa tgaaataatt tcattattta aagatgttgc
    17881 aacagaagta atagataaac aagtgatagt aattcatgat aaaagttatt atcataaaaa
    17941 gaaatctaaa tttcctattt ggggtaacag agaaattatt gcataccatt taggtataag
    18001 acacaaagta agcggtgtgt tattacaatt aaaataactt tctagtatgt gtggaattgt
    18061 aggagttttt agttataagt gtagaacaga aaaatacaat acttatctaa gcaagtgttg
    18121 taaatctatg aaacatagag ggccagatga tagcggatta tggtataata acgaaaatta
    18181 ttctgctgct tttgttaggc tttctattag agacttatcg ttaaacgggc atcagccaat
    18241 gatttcggct tgtggtaatt ttgtgattac ttttaatgga gaaatttata acagcaataa
    18301 ttatgttgca gaacttaaaa aattaggtgt acattttaaa tctaatagcg atactgaagt
    18361 gcttttatat gccttaatat attttggtat agaaaaagtt ttagcagagt ttgatggaat
    18421 ttttgctttt tctttttatg atgttcaaaa aaataaatta tttattgcta gggatagagt
    18481 tggtacaaaa cctttgtaca ttggctttaa caatgatttt ataatataca gctctcagta
    18541 tgatcatata attaatattg attttataaa aaataatagt ttagattatc aatcactagg
    18601 caattatctt tcatttggtt atatacttgc tggcgagggt gttattaaaa aaactttttc
    18661 tttaccgcaa ggccattatt tagaaacaac aaacgatagt tttttaattc ataagtatta
    18721 tgaatttgat gaaaaaaaga atatttctat aagcaatgta gaggcattat ttgaagaagc
    18781 aactatttct caaatggtaa gtgatgttcc tttaggcaca tttttatctg gtggggtaga
    18841 tagcccttta attaattttt gggcaaataa aaagcaacag gtaaaagctt tcaccatagg
    18901 aaatgaaaca gatgaattag atgaaagcta ctatgctaaa gcctttgcaa ataaaattgg
    18961 tgtggagcat catttcaaaa aaattactga aaacgatttt ctacaattat tggaggataa
    19021 ttttaaagct ttctcagaac catttgccga tttctcttct attccaacta tgttggtttc
    19081 taaaatggca aaagaacatt taactgttat tttaagtgga gatggtccag atgaattatt
    19141 ttggggatac aaccgtaacg taatttttcc aaataaagca acacttttta atcagggaaa
    19201 attatcgttg gctgtaaata aaattttagg gaagggtaac atttctaaaa gatattttac
    19261 tgccgattct attgcacatt tttatttaca gtctttgcaa attcatgggg cagaggcttc
    19321 tttaccaaaa gtgtatctag aaaaaattgt aaacaataaa atattttctt tattgccaga
    19381 acgtttgcaa caaccaaaaa atatgaacga tattatgcaa atggttagat ggctagaaat
    19441 gaatattcat ttgcaaagga tacttttaaa agtagataga gctagtatgt tttatagttt
    19501 agaagcaaga gtaccttacc tttctaatgc tgtattagat tatgcatcaa cacttaatta
    19561 ttcagattgt attaatgaaa atcagggtaa ggttaacatg aagagaattt tagaaacaaa
    19621 agtggataag gaatgggttt ataaaaagaa aaaagggttt gatataccaa tgagacaatg
    19681 gattcaaaat gaaattaaac aagatgtata tgaaactata ttaaatatgc caacagaatt
    19741 ggcttgtgtt tttgagatag ataatctaga aaatatttta caaaatgaat atgatgcctc
    19801 aaacaaaaat tctaattatg gatttatttg ggctgtttat tccttagtaa aatggcatca
    19861 aaatcatcgt aattcatcaa ttttaaagga ttaaataaaa tgaatattgc catcgttgtt
    19921 aataattttc catccaacag cgaaaccttt attattaaca aagtaattga attggctaaa
    19981 gctaaaaatt cagtaacagt atttagttta aatggcactg gcaataagga tttatatgaa
    20041 ttataccatt tcaaaaattt acccaacatt tcaattaagt atataaatct acctaaaaat
    20101 atttttgaat taattaagtt ttttttaagt aaccctttac ttaccattag ttctttttca
    20161 gccactccaa aaatattttt cagcaacata aaatctacat tactcaccaa acattttaat
    20221 aataagtttg atatagttca ttttgaatat tcaggtttag gagcttcttt actagaaatt
    20281 attcctttag taaaatctaa aaaagtagtg agttgcagag gtagtgcaga aaaagtaaag
    20341 cctttaacag acgctactag aaaagaaaag ttacaacaac tatttaattt ggttgatgga
    20401 atacattgtg ttagtgagga tatgaaacaa accattacac cttattgcaa taatattgaa
    20461 aaggtttttg taaacaggcc tgcaattgat gctacatttt ttcaaccacc tgctgtaaag
    20521 ccacaaaaca acattacaca aatacttaca gtaggcagat tcacttttca aaaagggtat
    20581 ttattgttat taatggttgc taaaaaatta gcaactaaaa acataaattt tgtatggaat
    20641 attattggcg aaggaccaca gcatgaagaa cttgtttttc atatacatac tttaggttta
    20701 gaaaaatgtg tgaaattagt aggaaagaaa aataagaatg aagtaaacca atatttagca
    20761 tctgcaaata tgtttgtact tacaagtgtt tatgaaggaa tacccaatgt agtattagaa
    20821 gcaatggcaa tggaattgcc tgtaatatct accaactgtg gtggtgtaga agaagtaatt
    20881 gaaacaggaa tagatggatt tatagcacct ttgtatgatg tagaaacaat tgctaatcaa
    20941 atagaatatt tgattgctaa accagataaa gctaatgaaa tgggtaggtt agccagaaag
    21001 aaaattttag aatcatttac tttagaaagg caagtaaata tttttgtaga agcctataat
    21061 aaaataatcg gttaagaatt tactgtgatt ttctctttta taaaatatac tcaaccagca
    21121 tggtacttta atgttctgcc aagtgtaaag gaattttctt gttgttatta tcatcctaaa
    21181 tggttgcctt taccaaatgg cattttgttg gatgaagaca ctgcttacga aactgaagca
    21241 gcaataaatg cagatattgg ttatagagca tggcatagtg gatatttaat taaagcatgc
    21301 aacaatgatt tacaaaaaat tattcagttg cctaaaccaa gcatcaatga tgagtatagg
    21361 tttgttacaa agtactgggg tatgcattgg gctgcactta cattagtaag aaggattttt
    21421 accttgcata atcctattaa agaaataaaa ggatttataa aaaatgtaaa cactccaaga
    21481 tataatttat acaatactgt tgctgattgg agtgaataca aaaatttcca atctgtttta
    21541 gtagagcagc agcctcttgt ctctgtcatt attccaactt taaatagata cgaatattta
    21601 gcagatgtaa tgcttgattt agaaaagcaa acttggaaga atttcgaagt aattgtgata
    21661 gaccaatcag aaaattttaa cgatgctttc tacaaaaaat ataatctcaa aattaatgtt
    21721 gttcaacaaa aagaaaaatt attgtggact gccagaaaca atgctgttaa actaagcaaa
    21781 gcaaattatt tattattttt tgatgatgat tccagagtag aaccagattg gattgaacaa
    21841 catattaaaa ccttagattt ttttaatgca tctatttctg caggagtttc cattgcagta
    21901 gttggtggca aagtttcaaa aagctatgat cattttcgat gggccgatca gtttgatagt
    21961 ggcaatgcgt tagttaaaag agaagtattt gaaaaaattg gtttgtttga tttgcagttt
    22021 aataaagaga gaatgggaga tagtgagttt ggcattagag cttatttgaa tgggtataaa
    22081 agcatctcaa acccttatgg caaaagagtt cacttaaaag taagtagtgg tggattgaga
    22141 gaaattggac attgggatgc ctttagacct aaaaaattat ttgcacccaa accagtgcca
    22201 agtgttatct atttatttaa aaaatattac ccaagttatt tgtcaaaaaa tgtaatttat
    22261 ttaggtatca tgctttccaa tatttcgtat aaaagtaaaa gtaaaaatag tatgcttgca
    22321 ttaagtgtat tgcttacctt tttaaaattt ccattattat acattcaata caaaaaatct
    22381 ttagcactag ctaataaaaa gttgcaacaa ggcgataaga ttgaaaaatt atagtttaat
    22441 ttaagtcact actttaatga atttaggaat tgtttcggat tgtgttcatt ttactacacc
    22501 aacaggtgtt gttgcaacag ataatcatat attccttcgt caacttaatg ccttagctgt
    22561 tcactttgat agagtaacta tttgctgccc ttttattgaa tttgatgata gcaaatccta
    22621 ttcggtttat accgataaaa aattccactt tattcctctt tccaatccag gtggtaatag
    22681 cataaaacac aaattaaaat tgttacttac cattccagag tggtttaaag catttagtaa
    22741 aataaataaa gcaagcgata ttgtttatca aagatttcct aataatctta atataccagg
    22801 cttcttttat ttttatttta aaagaaaaaa agtttttgct acttatactg gtgcatggaa
    22861 aaatgaaatt aatcaatcta aaacatacca atttcaaaaa tggttgttaa agaaattttt
    22921 tagagggccg gtttgggttt attcatctga agaaaagtta gctaaaaata tatttggcgg
    22981 ctttagccca tcttacacta tacaagaatg gaatgaagaa acagtgcaag taaataatag
    23041 aataaatttt ttaagtaaca ataaacttac atccttaaaa ctaatttctg taggggcact
    23101 taattcaaat aaaaaccaac aatatatttt aaacaattgt ttgttgctaa aacaagctac
    23161 catacccttt agtttacata ttgttggtga aggggtattg aaaaatgagt atcaaaattt
    23221 tattgaccaa cacaacctaa acaatgaaat tattttaaca ggtgctttaa attatacagc
    23281 attaagaaat ttatatagag aaaaagattt tgtggtacaa gcaacgttgt cagaagggtt
    23341 tggtaaagtg cccattgaag gtttttttca tggcttagtt cccatattgc atcaatctgt
    23401 tatggctccc tattttacca ataatggtac tagaggctac ttatttaatg cggaaaattc
    23461 aaatgatttg ttccatgtat taagtaatgt ttattccaca acttctgcaa aattaatagc
    23521 cgaaaaaatt aaagaaggaa ggatatttgt tcaatctcaa accttagaaa attgggcgtc
    23581 tatttatttt tcaacagtaa aaaaatattt tgatttataa agccattata tggaaatttt
    23641 tattagcact tggaatagga gtgattattg gggcaaaact aattaatgtt cagttatttg
    23701 gttttgctta ttttggtgct gttttactgc ttgcctattt tgctgttttt aataaaatag
    23761 aacaactgtt tactgtactt ccttttgtaa tttatagcga aatatttgta cgagacaggt
    23821 tagatagttt agtgccatat ttaacaattc aatacttata tatttttatt ttttccttca
    23881 tgcttattag gtttagtggt ggaaataaac cacacaataa agcattttat tttttattag
    23941 gctttgcatt tttagaaata ttcaatggcc taaacccaga taggccaaaa ctattacgtt
    24001 ctattttctt caatagtttt tcaatgattt tagcagttat ttgggcatct cataatgtgc
    24061 taacaccaaa gctaattaat aaaattttag ccaatgtaaa attagctggt atgtatttat
    24121 ctggtattgt aattgttgca caagttacag gtggtataga ttatggaaac ttttccaatt
    24181 cagaatcaag taatggttta gcacctgtac agttaagtgg atatttaagt gtggctgctt
    24241 gttttatttt cttttcttta atgaataaag atgaaataaa aagcaggtat gtttatatta
    24301 tagcattttc cattattaca ggtgtaatga ttttaacttt ttctcgtggt ggcttatact
    24361 ttttaggagc aattcttaca ctttatttat tttttaatag aaaaaattta ggtaactatt
    24421 tcaaatattt attgtttcta ccaattggtt tagttattta taatttagcc attgaaaaaa
    24481 cagaaggtaa aattattgct aggtacgaag ctaaaggcac atctaacaga gatatattag
    24541 tagaagctgc atttaaacta tttttagaaa acccagtagt aggtgtaggt acaagtaact
    24601 atggtactgc aattgtggag tacagattgt ttaatcaaga aagtacagca cataatgaat
    24661 ttgcaagagc tatggcagag catggcattt taggaataat tacttattgg ggatttttta
    24721 tttcattatt tattacaatt tttaaacgaa aagaacctgc caaacaattt tctgtttatt
    24781 ttattgcttt atttattttc atcaccattc acaatggttt aaaaataagc attcagcatt
    24841 tattaatcat tatggcaatt gctaatccaa ctgctgttaa agctattttt tacagaaaaa
    24901 taaaacctaa tactattgtc aaacaatcag ctatatcata ctaatagcag ccatttacca
    24961 caattggctt tatgtcatca aaaatgtttt aaaggctctt tgtcagaaaa attaggctta
    25021 gcttatattg ctaaaacctt agactggttt ttaaaagatg ataataagtt tttatttcat
    25081 tttcaaaata ccgaggggca agtaataggt tactgtggtg gttttgtttc taaaaaaaat
    25141 ggagatggaa gtagcagtgg tatgttgcaa catgctttta aagaggctgt aataggtatt
    25201 gtaaaaaaac cttggcttat cttcaataaa gaagttgtag caatgtaccc atttattttt
    25261 aagaatataa aacaaaaact ttttaaaaaa agtaaggcaa agcaggttgt tgattattca
    25321 aatgaagaaa aaaaatttgc tggcttggtt gttattggtg tacatcctgc ttatagaggt
    25381 aaaggttttt ttgaaaagct aatggagcaa ttttttattg atgcttcttt aaaaggagct
    25441 attggtggtg ctcttagtgt taaaaaaaat aatgcaagag gtattgcagc ttataaaaaa
    25501 aatggatgga taattgttag agaagatgca catacttaca tcatgcaaaa attaacccaa
    25561 acaagttaat gcagcaaagt atattactaa ttggtttatt tctttctgaa aagaataaac
    25621 ataaaattta tagaactgcc gcagatcaat tagcagaact tttacataag aataattatt
    25681 ctattataaa agcatccact tatgtaaata aaatacctcg ttttgtacat actatatttt
    25741 ctattatttt taattcttcc aaatattctg ttgcaatagt gccattgtat ggtggcacat
    25801 ttagtttgtt ttgggcagag gcagcaacag ttttcttaaa acttttccgt aaaaaaataa
    25861 tcttaatcat tcatggaggt tccataccaa ctagaatgga aaatgatcga aaagcaaaaa
    25921 attatttaag aacaatccaa aaagcacatg ttgtggtgtg cccttctaac tacatcattc
    25981 atcatttaca aaagcatggt gttaatgctg ttttaataga aaatgtttta aacttgtctg
    26041 aatattcttt tcatgaaaaa aaattattaa ggccaacatt attttggatg agaacatttg
    26101 aagatgtgta taatccgcta atggctgtaa gagttttggc tatcttacaa aatacatacc
    26161 catctgcaaa aatgataatg gcaggcagag atgcagggct tttagaagag acaaagcaat
    26221 tagcacaaaa attaaacgtt atagataaga tagtttttcc aggttatata gataatacag
    26281 caaaaaataa tatagcccaa gagtatgata tttatatttg caccaatagc attgataatg
    26341 caccagtaag cactattgaa atgatgagtt taggcttacc tgttgtaagt acaaatgttg
    26401 gaggtattcc ttttatagta actaataata aagatggttt attagtgaac ttgaatgatg
    26461 acgaaggaat ggtaagtaaa attgcattac taatagagaa tataaatatt gcagaaaaga
    26521 taattcaaga aggcttaaat acttctaaaa aatacgatga agaaattgtg ataaaaaaat
    26581 ggaataaaat ttttaaacaa ctgaatttta cataatggat aaaataaaat ttgcattaat
    26641 tggatgtgga agaattgcac aaaggcatgc agagcatatt aataaacttg gtatattaaa
    26701 agcagtatgc gatacagtag aagaaaaagc aaaagcatta gcagaaaaat ataatgtgcc
    26761 ctattatacc aattttgaag aaatgctttt aaaagaaaag gaggtagatg ttgtatcaat
    26821 ttgtacacct aatggtttac atgcaacaca tgcaattgcc gctttaaatg caaattttca
    26881 tacacttgtt gaaaagccaa tggcactatc tgtttacgat tgtggagaaa tgattaaagc
    26941 tgcagaaaaa gcaaacaaac gtttatttgc aataaaacaa aatcgtttta acccgcctgt
    27001 agttgctgtt aaagaagcta ttgataataa tattttaggt aaaatttaca atatacaatt
    27061 aagttgtttt tggaatagaa ataaagatta ttacgaaaac tcttggaaag gaacacatga
    27121 tttagatggt ggaactttat atacacagtt tagccatttt attgatttgt tgtattggat
    27181 gattggtgat gtaaaaagag cttatgcact cacttcaaat ttttcacatc aaggcattat
    27241 agattttgaa gacacaggtg taatcgcatt agagttttat aatggtgcaa ttggaaccat
    27301 aaattatact gtaaatagct atcaaaaaaa tatggaaggt tccttaacca tttttgcaga
    27361 aaaaggaaca gttaaaattg gcggccaata tttgaatgaa ctagaatacc aagcaattga
    27421 aaattttgaa ataaaagatt tacctacagg caatactgct aataactatg gtacttatgt
    27481 aggtagtatg agtaatcatg ataaagtgta tgaaaattta attgatgtgt tacaaaatgg
    27541 taaatcaatt gctaccaact catttgaagg tttaaaaacg gtagaaatta tttctaaaat
    27601 ataccaatct gccaaagaac ttaataaata atattgaagc aaattaattt taataattat
    27661 gaatattcct tttgttgatc ttaaacaaca ataccaatca ataaaaaatg aaattgatac
    27721 cgcaattgaa aatgtattaa ctgaaactgc atttataggg ggtaaatatg taaataagtt
    27781 tgaaaaagat tttgaaacat tgtatggtgt aaaccattgc attagttgtg caaatggtac
    27841 agactcatta tacataatta tgaagatgct tggaataggt gctggtgatg aagtaattac
    27901 tgttgccaat agttggatta gtagcagcga aacaatttct caaactggtg caaagcctgt
    27961 atttattgat attgaggaag aatatttatc tatcaatgaa aaattaattg aacaaaaaat
    28021 aacttctaaa accaaagcat taattatggt gcatttgcat gggcaaatgt gtgatattga
    28081 aactatagct caactatgta agaaatatca ggtgtattta attgaagatt gtgcccaatc
    28141 tcatttaagt gagtataata atataagagc tggtttaaga ggtattgctg catcgtttag
    28201 tttttatcct ggtaaaaatt taggggctta tggagatgca ggttgtatta taactaatga
    28261 ttcggccttg gcagaaaaat gtaaaatgta cgcaaggcat ggtgctttaa taaaacacca
    28321 acataaaata gaaggtatca actctaggtt agatggctta caagctgcag tattatctgc
    28381 taaactgcct tttttaatag agtggacgaa taaaagaatt gctgctgcaa aacattatga
    28441 tgaggcttta aaaaatataa atgaaattgt tactccaaaa gttagagcta actcaaaaca
    28501 tacttttcat ttgtatgtta taagagcaaa gcaaagaaac gaattggcca attatttaaa
    28561 ggaaaaaggg attgaaactg ctattcatta tccaacacca ttgccatttt tagaggctta
    28621 caattattta aattataccg agctggattt tccggttgct aataaattta aaaacgaaat
    28681 tttatcatta cccatctatc cagaaataac aatagaacaa ataaattata ttgcattaac
    28741 cataaaatct ttttatagta aatgataata tcaattatca gcccggtttt taatgaagaa
    28801 aaatatatcc atcaaacaat aaaaagtttt ttggaacagg agcatacttc ttttgatgtt
    28861 gaaatattgt tgtgtgatgg aatgagtaca gataaaacca gagatatcat taaaagctat
    28921 gcagaacaat atgccaatat taggttaata gataatccaa aacgtaaaac accttttgca
    28981 tttaatatag gtataaaaga agctcgtgga gagtatgttg ccattttagg ggcacatagt
    29041 gcatacgata aaaattattt acaggcttgt tataatgagc ttattgcaac caactccata
    29101 gcatgttcgg gtagggtaat tactcaatct gcatttgata ctttagaagc taaattatgt
    29161 gagtgggcaa tgcttagcca gtttggggtt tcgggtaatt cttttagatt aatgaaagaa
    29221 ggctataccc attctgtaaa ttttcctgta gtaaaaaaag aagctttact aagcttaggt
    29281 ggttataacg aaatgttaga aagaaatcag gataatgata tgaaccagcg tttaatagat
    29341 gcaggctatc aattatactg tacctggaaa acaaaatgct tttacaggcc tcctgctact
    29401 ttaaacaaac tatttaagta tggttataga ggtggttttt ggaatgcaaa aagttttttt
    29461 gctcacaaaa aaagcatgcg tattcatcat tttattccat tcttttttgt tgtagcttta
    29521 ttaactactt ttactttagg tgtagtagag tttgtactag gtaagtcaag ttattttttt
    29581 agtttgtttt cattaattgt attctttcac cttcttgcgg ggcttttctt ttctgctatt
    29641 tcactaaaat atgaaagtga tagtagaaaa atatttttgc cctttgtgtt ttttttattc
    29701 cattttagtt atggatgggg aactgtaaaa ggttttgttg attataaaaa ataacaattt
    29761 ttaatttcca attatttttt ttatgtatta gtataacttt cttccgatga atagttgcta
    29821 aagtcttcac cctcttttgc attattattt aaaatagtag ctaactcttc ctctgctttt
    29881 ttaatagctg ctattctaag tttatctcct acaatccata gtatatcacc agtttttatt
    29941 gtatatacag attctggatt aactattctt tctccatttg tttcaactcc tacaactatt
    30001 ccatgtgttt gttctcttat tttcgattct ctaattgttt tatttacaaa taaagaatta
    30061 ggaataataa ttaattgctc taaagttatt tcatttcttt ttacttgtgt tgtagattcc
    30121 tttttattca ccacttctgc tttcataaaa gaactaaact gcattaattg ctcatcggta
    30181 ccaataatgg ataatttatc ggatggaaaa attctttcgt aacgtccagg cgtgtttatt
    30241 attttttctc ctctaaaaat tctggcaata ttaattccaa aatcttctct ccagcccaat
    30301 tctaatagag tttgaccaat accattaaaa ttagcatcaa cagtaaagtt tacaatatgt
    30361 gcatcccatg gtgcaagttc agattgaact tctgtaattt taatagcctc tcgttcgtta
    30421 taattaagta aaaactgatt ttttaattta ttatataatg cttgaatttt ttttgaaaaa
    30481 ataattaaaa ttataaacat aataatagaa acagtaagag caatttttgg cgaaaagaat
    30541 ctatctaata aaaagccaat caaaaaaatg gctatggcaa ttcttataaa atgtaataca
    30601 tacaagctac ctctaaagcg ttgtatttgg gcaagttctt ttaatttatc ttttttgaaa
    30661 ggtattacta aattccataa aaaaggtgcc attacaatta gtgctaaaat agaaccaaca
    30721 attgttgccc aagtattatc gcctaatttt gttcttgtat atggtaaaat ataataagag
    30781 gatagtagta ctacaaatga aattaataca gttaataaaa acgaactaat aatccaagaa
    30841 cgaacatact cttgccattc gtttttgata gattgttttt gagaacggtt actatatctg
    30901 ttaattgctt ttacccagtt aactggtaaa atttttacaa tccaattata gaaggggtct
    30961 gatagtttta ataaatatgg tgtagtaaat gttgtaatag cagatactgc aattgcaata
    31021 gggtaaataa aatcgctagt tacatttaaa gaaaggccca ggcttgcaat aataaaagaa
    31081 aattcgccaa tttgtgtaag gctaaaccct gcctgaacag atgtttttag agcttgccca
    31141 gaaagcaatg cacccaaaga agcaaagaaa aatttaccac ttataattac tacaatcatt
    31201 aatataatta tgcctgcata atctaccaca atttgtggat taaataacat accaaccgat
    31261 acaaagaata ctgcaccaaa taagtcttta acaggtttta gcaagtgttc aattttttct
    31321 gcatataccg tttctgctaa aatagaaccc ataataaagg ctcctaatgc aggagagaaa
    31381 ccaactcttg cagctaacca aaccattaaa atacataatg ctaaagatgt tatcaaaagc
    31441 atttcatcat ttaataatct tttggctttt cttaaaattg taggtacaaa aaatattccc
    31501 aatacaaacc acaatgctaa aaagaaaatt aatttaacta cagaaaataa catttcactg
    31561 ccagaaaatg tactacttat agaaagggta gtgagcaata caagcaacac tatggctatt
    31621 aaatcttcta ccactaaaat tccaaacact aagcctgcaa actgctttcc ttttacaccc
    31681 aattcatcaa aggctctcat aataatggtt gtagaagaaa tgctcattac accacctaaa
    31741 aaaatactat caatttgttt ccagcccatc agtttaccca gcataaaccc caaaaaaacc
    31801 attccagaca cttcaattat tgcagtgata gaagctggtg ctcctacttt cattaatttt
    31861 ttaaaactaa actctaaacc caagccaaat aataaaaata ttacaccaat ttctgcccat
    31921 actttaatgt ttgctacttc tgcaatagag ggtgttaaat gtaaatttgg cccaactaaa
    31981 aaacctgcaa ttaaatagcc taataccaag ggttgcttta accatttaaa taatatggta
    32041 actattgctg ctgcacctaa aattaaacct aagtctgtaa ttaattgcgg tatatgattc
    32101 atgttttggc tggcttatga aaaagtatgc tttaagcatg taagataatt ttttttattt
    32161 aatttttgta atttgctatt catgaaaata tttctaatta aaaaacccac tgaatttatt
    32221 caatgggttt tggttataat tgttttatta ttatctattt ttct
//
LOCUS       DAOQPK010000003        31276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_644_length_31276_cov_4.877038, whole genome shotgun sequence.
ACCESSION   DAOQPK010000003 DAOQPK010000000
VERSION     DAOQPK010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 31276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31276
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_644_length_31276_cov_4.877038"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   13..906
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=106..138
                     /rpt_unit_seq="tagaaagaacaaagtttgaaagcaaatcacaac"
     gene            complement(934..1110)
                     /locus_tag="PLU36_00330"
     CDS             complement(934..1110)
                     /locus_tag="PLU36_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45225.1"
                     /translation="MQLILTVNMTPQQKKVLDDAVKTKLKNEKILTGGNKYINPIRKV
                     PKKKKEGLIEKPGN"
     gene            complement(1231..3099)
                     /gene="mnmG"
                     /locus_tag="PLU36_00335"
     CDS             complement(1231..3099)
                     /gene="mnmG"
                     /locus_tag="PLU36_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HMZ45226.1"
                     /translation="MFLNYDVIVAGAGHAGCEAAAAAANMGSKVLLITMNMQTLAQMS
                     CNPAMGGIAKGQILREVDAMGGYSGIVSDKSMIQFRMLNRSKGPAMWSPRTQNDRMVF
                     ALTWREMLEQTPNVDFYQDMVKSLIIKNGTVYGVVTNLGHEIYAKSVIITSGTFLNGV
                     IHIGEKQLGGGRISEKSASGITEQLVEVGFESDRLKTGTPPRVDGRSLDYSKMEVQHG
                     DDKISGFSFLEIERIQPQQQKPCHITYTNSTVHEILKTGFDRSPMYQGRIEGVGPRYC
                     PSIEDKINRFAERDRHQLFVEPEGWNTVEIYVNGFSTSLPEDVQVKALKMVPGFENCK
                     IFRPGYAIEYDYYPPTQLNHTLETKIIHNLFFAGQINGTTGYEEAACQGLMAGINAHQ
                     KVNNLEPLILKRSDAYIGVLIDDLISKGTNEPYRMFTSRAEYRTLLRQDNADLRLTEM
                     AFKLGLASLERMEKVSYKKNKIEEIKASLNDVYIEPAEINEYFEKIGSAVMQEKQKAS
                     KIILRPGVNLNELLNFSLILKNKYSNEKEDIIEQAELQVKYQRYIEKEKDLVDRMSQM
                     EELIIPDTFDYNKVIALSNEALQKFKKIKPRTLGQASRISGVNPSDIQILMVYMGR"
     gene            complement(3219..3653)
                     /gene="ybeY"
                     /locus_tag="PLU36_00340"
     CDS             complement(3219..3653)
                     /gene="ybeY"
                     /locus_tag="PLU36_00340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HMZ45227.1"
                     /translation="MKSKVYFNNADRNLVYKNKTNLKQFVATIFELEKIELETINYIF
                     CSDEYLLKINQQFLNHHYYTDIITFCLSSPKQPIISEAYISIDRVKENAYEFNTSFNN
                     EIRRVVFHGALHLCGYKDKSKKSIDLMREKETFYLNMFNSQM"
     gene            3870..4442
                     /gene="gmk"
                     /locus_tag="PLU36_00345"
     CDS             3870..4442
                     /gene="gmk"
                     /locus_tag="PLU36_00345"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HMZ45228.1"
                     /translation="MISEKIIIITAPSGAGKTSITKYLLNKYSNLSFSISAATRLPRK
                     GEVDGKDYYFISTEDFKNKIKNNAFAEWEMVYEGKYYGTLKSELENIWKANKIPVLDI
                     DVKGAIHIQQAYLNKTLSLFIQPPSIAILKERLESRGTETEETIQTRINKAAYEISFC
                     HSFDKIIVNDNLETACKEAEKIIETFLCQS"
     gene            complement(5066..7168)
                     /locus_tag="PLU36_00350"
     CDS             complement(5066..7168)
                     /locus_tag="PLU36_00350"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HMZ45229.1"
                     /translation="MSIIQNIREKGAWLTFGFIAVALIAFILMDGVGRGGAGMTATST
                     IGKVNGTAIELGDFDQKLNMYSQGGQDRNSILPQLWNMEVERILLEQECEKLGITVTG
                     KEVGEILFGENSPLKREKQFVDDNGNFKAEEARQAFAQLKNSKNNDNLQGVIQTYVEP
                     IKQQALKAKYDAILQQSVYVPTWLVEKTKADNSGLAKINYVFVPYTSISDSTVKDISD
                     NEIAAYIKKHPAGFEQKEEGRSFSYVVFSTAPSAIDSLNTLNNILSLKPEFAKEENAE
                     AYLGRIGTEIPYLNAFLPKKDMKMPNADSIKALADGSTFGPYLDGGNLVIAKMIGKRN
                     LPDSVKVRHILVKTEDRRNPVLEDSIAKKRIDSVEALVKSGADFNQLVQKYSDDGGSK
                     ATKGEYDFALQQFSGISKEFAETIFYGKAGDKKIVKVENDAYSGYHYIEVISQKNFSD
                     AVKVAYLAKPILASNETMNAANSAALQFAAKIKNKKQFEEEALKLNKSVMIAQDVKEN
                     DFTIPNFQQASVRNLVRWLYEKKVDAVSEPMEIGENFVVSILTGVNKVGLKSVASARP
                     MVEPFVRNEKKAKLIIESKFKGSTLEEYASSSGTSIQSSDSVYFSNPVIANMGYDLKV
                     TGSSFNKDMVGKVTPPMASNAGVVAVKVITIGTIPPVQDDAAIKQNILGQMRNAAFRS
                     AQALRNAATIKDYRFKVY"
     gene            7724..8731
                     /gene="nadA"
                     /locus_tag="PLU36_00355"
     CDS             7724..8731
                     /gene="nadA"
                     /locus_tag="PLU36_00355"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HMZ45230.1"
                     /translation="MNINIAKEQVAEKGFLDIEVSPTLDLFAEIEKLKKEKNIVLLAH
                     YYQEPYIQDVADYIGDSLGLAQQAAQTNADIIVFAGVHFMAETAKILSPSKKVLLPDL
                     NAGCSLADSAPPKLFKSFKDKHPDHIVVTYVNCSAEIKALSDIICTSSNAEKIIESIP
                     KEQPIIFAPDRNLGAFLNKKTGRNMVLWNGSCIVHEIFSLEKITKLKIRHPKAKFIAH
                     PECEEAVLNVADFIGSTSQLLSYTEKNEATEFIVATEAGILYQMQQNSPQKTFIPAPP
                     NNSCACNDCPYMKLNTLEKLYLCMQYETPEITIDEKLRLQALKSLDRMMEISKAAGLI
                     K"
     gene            complement(8736..9401)
                     /locus_tag="PLU36_00360"
     CDS             complement(8736..9401)
                     /locus_tag="PLU36_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HMZ45231.1"
                     /translation="MAQICKIISLELLTQDVLKLIVEKPENLHFNPGQAVDVSINKPN
                     WKDKIRAFTFTSLPTDNFVEFIIKTYPQHNGVTNELLFLSVGDELIIGDVFGDISYKG
                     NGVFIAGGAGITPFLSILKHLQQQNKIANNILLFANKTKADIILEPFFKNLLKENFVN
                     ILSDEKLDNYEHGFINKTMLEKYTASNNIFYLCGPPPMMQAVEGFLIKLNINPENIIK
                     ESF"
     gene            complement(9408..10052)
                     /locus_tag="PLU36_00365"
     CDS             complement(9408..10052)
                     /locus_tag="PLU36_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ45232.1"
                     /translation="MEELIKLSDIEIIDSILNGNKHLFEIIVRRYNPYLYKIGRSYNY
                     THEDTQDLMQESFIDAYKNLQQFSGKSSFKSWLIKIMLHNCFHKKGKLNFKKSSMKEI
                     NENDKPIFNMETKKLNYENKELSHILENALTNLPLHYRMVFSLREINGFNINETADIL
                     NISETNVKVRLNRAKSMLRSQVEKVYSKAELFEFNLIYCNAIVNRVMSKINNLK"
     gene            10181..11128
                     /locus_tag="PLU36_00370"
     CDS             10181..11128
                     /locus_tag="PLU36_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HMZ45233.1"
                     /translation="MKNPVICIGAALVDELYSATRPMLMSTTNDAFVHRSAGGVARNI
                     AHQLALLNVPVQLISVFGNDGEGDWLKTICSYVNIKLDAAITNQALTGKYTGILNYDR
                     SLFTALLTNTSLDAITPQHLQQHESLLSTAFCIVADANIHPDTVAWLAFFCFKKNIPF
                     IIEPVSVPPAQKFAKMELKGIQMLTPNEDELPAMCSGGGTTFEENAIELLNRGVEKVW
                     LHRGKRGSMLFTKENTYSLEAPPVDDADIKDVTGAGDGSLSGWLMGKYLGKDDEDCLK
                     ISHTLSAEILQVNGAIATHLDQEKLLSLVTKYYQQRNFL"
     gene            11191..14043
                     /gene="polA"
                     /locus_tag="PLU36_00375"
     CDS             11191..14043
                     /gene="polA"
                     /locus_tag="PLU36_00375"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HMZ45234.1"
                     /translation="MTEKKLFLLDAYALIFRAYYALIRNPRITSNGKNTNAQFGFTNT
                     LVELINKQKPTHIAVCFDVGGSVIRETEYADYKANRQETPEDLLAAVPDIKKIIEGFN
                     IPVIGIEGYEADDVIGTLAWEAHDAGFQVFMVTPDKDYGQLVRDGISIYKPATQGNDV
                     EILGPKQVCEKWDIERVDQVIDILGLMGDAVDNIPGIKGVGEKTAAKLLKEYDNLENV
                     LANAENIKGALGEKVKAGKADAILSKKLATIITNVPVSFNEEEFKLKEWNKEALNEIF
                     AELEFRTLGKRLLGEDFTIASNNVQTDLFGNIVESKTPKSEKKQKETTKEQEEFNLIN
                     TTVDEENKAFKNINNTTHNYILIDSEEKIDNLIKQLLQQTEICFDTETTNIDANNAEL
                     VGISFSYKPHEAFYIPLSQNFTQAQQILEKFIPLFNNTNIVWIGQNIKYDLLVLKWYN
                     IQIQGNVFDTMLAHYVVEPEGKRSMDILSEQFLQYQPIPIEDLIGKKGKTQGNMRDIE
                     VEKIKEYAAEDADITLQLKQKIEPLLKEKEVEKVFYEVENPLVKVLTAMEFEGIKIDE
                     AFLKEYSKELSKEAKAAEEAVYKKSEVRFNLASPKQLGEVLFDKLKLDSSAKKTKTGQ
                     YQTGEDILQKLSAKGHSIAEDILVYRELTKLKSTYVDALPEIINSKTGRVHTTYGQAV
                     AVTGRLASNNPNLQNIPIRTAKGREIRKAFIPRDTNRVLISADYSQIELRIVAAISND
                     ENMCAAFRAGKDIHTATAAKVYNVAENEVTKEMRYKAKSVNFGIIYGQGAFGLADNLG
                     ISRTEAKEIIDNYKREFPGIQKYMDDTINFARENGFVQTIMGRKRWLKDINSSNFTVR
                     GFAERNAINSPIQGTAADIIKMAMIKIHTALQKEQLQSKMILQVHDELVFDVLSSEIE
                     LIKPLILNNMKSAMVLPNQVPVEAELGIGNNWLEAH"
     gene            14237..14905
                     /locus_tag="PLU36_00380"
     CDS             14237..14905
                     /locus_tag="PLU36_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45235.1"
                     /translation="MAQAQNTQELNNQSASNNLQTIYHKTVQYKQNVFTGIEYFRNYG
                     KINGHAFWNENEFINGNVGYDGNIYPNLPLKLDLMVEELITHGVNQGVQIKIIREKID
                     SFTIGSVRFLHLTPDKNKVPDGFYEILHSQKYTVFCKRNKLIKEVIVQNELERNIEEE
                     AVYFLKNQDNIIVIKNRQILFDLFRDKKNQVLDYMSNLNLNFRKQKEAFIVESIKYYE
                     QITN"
     gene            14910..17675
                     /locus_tag="PLU36_00385"
     CDS             14910..17675
                     /locus_tag="PLU36_00385"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMZ45236.1"
                     /translation="MKFSLRLLTLLALIILFSYAKAQNENNITLDLKRASFAEFVQNV
                     EKQTNYFFYYNKVDTDSVFITISVQNATIATVLNTVFENTELYFSIADNKKIFVSRNV
                     QLLTNLGTLFFKTNEQVNKKEKTITVIEDSIEETNTNDVAIQNKLIVIGKPNAAVSGK
                     VLITGQVKNGKNNEPIVGAVIAAGKTNVVTDYLGKFTISLNKGRNMLTASSIGMRDAV
                     RQLLVQSSGNINIEMQENITALKGVVVTATKRNTGVKTPKMGVEKITIKQLKQQPVLF
                     GEADVLRAVLTLPGVTSVGEASTGFNVRGGSVDQNLILFNDATIFNPSHLFGFFSAFN
                     PSVVKGVELYKGSIPEKFGGRISSVLDVSVQEGNKKKFGGAGGIGPLTSHLMFEGPIK
                     KDKGSFIIGFRTTYSDWILKNLKDVSFKNSKAAFNDLSIHLSQQINTKNFLYITGYTS
                     KDNFRLDKDTSYAYKNNNINIKWKHIFKNNLISNVTAGYDNYNYGIESSLKPLSAFTL
                     KFNINQYYLKADFTKSYGNVHRINFGYNGNFMKLNPGSYAPSGKVSLVNAINIQQEKG
                     IENAIYLGDKMELSSKLAVEAGIRVSAFTAIGSSKVYKYYNGLERDDANIQDTIFYKN
                     GQPIKTYWGPEVRLSTRYTINETSSIKAGFSTQRQYIHMLSNTTAISPTDIWKLSDTY
                     IKPQIGQQVSLGFYKSFPKSDVEVSVEVYYKWLQNFLDYKSGALLLLNQHIETDVLNS
                     KGKAYGVEFMIKKNSGKLNGWLSYTYSRTMLQTDEPTTSYKVNKGSYYPANFDKPHSI
                     NFVGNYKFSHRFNVSLTTVYSTGRPITLPIAIYNYGGGDRVYYSDRNQYRIPDFFRAD
                     FSMNIEGNHKIKKLAHSSWSLGIYNITSRKNPYSVYFATENGKIKGYQLSIFGSAIPF
                     ITYNFKF"
     gene            17692..18828
                     /locus_tag="PLU36_00390"
     CDS             17692..18828
                     /locus_tag="PLU36_00390"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HMZ45237.1"
                     /translation="MKRHIIYLVTIVAIITGCRENYTPPEVTTDHKILVVEGFIDVNK
                     DSSYFKLSRTQKLGSVSSVKPEAGANISILSSNGTVLTQFKERANGQYVVYGATLLPT
                     EFYRLKITTSGAVVYQSDLIKGKITPAIDSVTWKIDNDKNGIQFYVNSHDAANETKYY
                     KWDCEEVWEYRAYYESQYKYLGDTIIEPRLPGEQIYRCWRTVLPTQIFINTTEKLSSD
                     VISNEKILFVGKGSNRFDQRYSILVKQRALTKEAFEYWQQLKQSSELGGSVFDVQPTQ
                     LLGNLHCVTNPNEPIIGYVSATTTAEKRIYILNTNVPTFVQPYPATCEFAVVANNKDS
                     MKVYYTNKRYIPIAPDMQGAYLAGSTQCTDCRVLGGTTIKPAFW"
     gene            18870..21236
                     /locus_tag="PLU36_00395"
     CDS             18870..21236
                     /locus_tag="PLU36_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45238.1"
                     /translation="MQKLTFSVLLFLAFSFKLFAQVDDRKDVSLKFNEYVLNNFQEKI
                     FVHTDRNFYLSGDIIWFKIYNTSASQNTFTTISKIAYVELLNTENKSLLQAKIALTDG
                     TGNGQFVLPLSLPTGNYIFRAYTNWMKNYNEEIMFEKTISVINAFEKNSIDTAKKAIS
                     YDVQFFPEGGNLVNNIQSKIAFKVINSNGFGVMTKGKIVNQNNETIQEFNTNHLGIGS
                     FLFTPVNTNTYKALVFTPTNDTLSYNLPKILNDGYVMQVTRNSTHTTITVFASNYLNN
                     KAVKLVIHNNNTIQYNQTQSINNQQAVFTVHNNLLKEGINHLTIFNDYDLPVTERLLY
                     KRPTEKATLKTNINNTVFNNREKVNIMVNGVTNTNQPIETNLSVAVFKTDSFQLNNDV
                     DIQQYLWLTSELKGNIEEPAYYFNTINEETDSALDNLILTQGWRRFNWLNILQSPTTP
                     KYLPEYENHIITGKVIDKRTLQPAEGIGIYLTIPGKEFGFAAAQTNSEGNFFTLMPTI
                     YNSSELIAQTNNRTDSNYRIDINSAYWEKTTGYKPTKFLISKNNERLLNNYGISVQAN
                     YIYHSKPLYKETQAYSPFYGKADEVYFLDNYTRFSTMEEVMREYVTGVSVRKRAGKFI
                     FHTVDAPRFQFFTTEPLILLDGIAVFDADKIIATDPLKLKKVEVTTRKFYYGSFAFDG
                     ITSFTSYDGKFGKNDLHTESLVIDYDALQQEREFYAPKYDIVENKESRVPDYRVLLHW
                     SGTIKTTTNGATDIEFYTSDLGGKYTAIIQGITNTGNPVKSMVEFTVK"
     gene            complement(21238..21954)
                     /gene="kdsB"
                     /locus_tag="PLU36_00400"
     CDS             complement(21238..21954)
                     /gene="kdsB"
                     /locus_tag="PLU36_00400"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HMZ45239.1"
                     /translation="MKISAFIPARYAATRFPAKLMQMLGNKTVIRHTYDNTLATGLFN
                     EVWVVTDSAIIYNEIVNNGGRAVMSVQEHESGSDRIAEAVKNIDVDIIVNVQGDEPFV
                     KKEPLEKLLNVFEDSSVQVASLMQVLKNEQQINDPNFVKVAVDKNMNSLLFSRSVIPY
                     HRDKNIAPTYYEHIGVYAFTKQALLHFTQWEITPLEAAEKIECLRYVENGIALKMVVT
                     DYMGIEIDTPEDLINATKFL"
     gene            complement(21985..22755)
                     /locus_tag="PLU36_00405"
     CDS             complement(21985..22755)
                     /locus_tag="PLU36_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HMZ45240.1"
                     /translation="MRIISYNVNGIRAAIKKGFIDWLKTNPADIICLQETKATISDID
                     LQPFQELGFEAYFFSAQKKGYSGVAVFTKLKPNKVVYGTQIEQSDFEGRVIQLYFKNF
                     ILINAYFPSGTSGDERQTYKYQWLNEMYDYVQALGKEHKNIVLVGDYNIAHTEIDIHD
                     PKGNKNSSGFLPEERAWLTKFLHTGWIDSFRFKNPDTIGGYTWWSQRFPSVRLQNKGW
                     RIDYICVTNALKNKIKTASIFADIKHSDHCPIFLELSV"
     gene            22855..23586
                     /locus_tag="PLU36_00410"
     CDS             22855..23586
                     /locus_tag="PLU36_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HMZ45241.1"
                     /translation="MKNKDKIIPSREHYYLEGPKHRVNELVWVWSIFVEFIGAIRKLH
                     FTGPCITVFGSARFKEDNEYYIAAREFGKRIAQLGFTTMTGGGPGIMEAANRGAFENG
                     GYSIGCNIKLPFEQKENPYINKSYTFNYFFSRKVLLVKYSYAFIIMPGGFGTMDELFE
                     TLTLIQTKTINNFPVVLYGKKYFQPLINYIEEMIEQKTISKEDLNLLLVTDDFEEAIN
                     HIKAYVHKHYKVKPRKRLWWLFEKK"
     gene            complement(23592..28331)
                     /locus_tag="PLU36_00415"
     CDS             complement(23592..28331)
                     /locus_tag="PLU36_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HMZ45242.1"
                     /translation="MKLVLLSTFLIFHLFVFSQTNSEIDFKAHFEKGTNLLLAEKYDS
                     ALYYFNLSEKIAEKQNKYSDDKYSSLNINKGICYMYLQEPDNAHYYMYKGLLNARKFK
                     HIIPESNAILFLNNLHNTILEKNWTFNYPKMTTTFGSYIYFPILQIDSFKNSDSLKII
                     VAAGKYDGIANDDYKVNIYKRYDDKYKNEEDVKNSYSIGIGRVVSVENNRTIIYIKSS
                     STVPIEPKDLANIYCQTPVSAKKNKFVPLLDIGINLLDNTKEKLLLSRRFLLYYDDEK
                     IHFELIKNLKVELFYVQNAYAEDTLKPNNTLAQKATEGIFKGVNIIKAFDSTNTKHIV
                     AFINFMKAYPGKYIGNNFSFSEIYATWLLNNTPMANDDLLLYIAQTEKNKRAEIAYAL
                     KNQIKQNNIIEKWVTLTLNAFDKNDLLNVRIALMSFAAVYESEKNIDVGGWLSFLNGL
                     FQYKTGSVSLADTALKKAEKFFTKSNNKEGLTITKKALQKIHTNNKVSLEVQLGEFAS
                     YSVAMHPSGKYYATNDANNTIRIWDLHLGKTIHTFQPQNEIVYSIAYSPNGRYFATCS
                     DNKTIKIYSTFDYAVITTINTSNTEYCIAFSPNNKEIASGGYDSLIKVWNFITGEKLY
                     SLNKHKGAVTQLCYVGRNEDILFSSGTDSMVYRWNLQTKHDVNWYRKKAKLLNMKVSY
                     NGNYLFYTANDSSINVWNLYKNKFYFKEKIAVAQYGDTKFYNEPDFSPDGSLLTYANQ
                     QNSVVLVDLNTGYLKAITKTANKYAYLNTLNFTTDQMGIFATYPYISKTKLFDISGYK
                     NIHSFNTNVELKINKQYTNPNITLQFSAKDDALFTVGERIVKFDFSNNSTHQLFYAAQ
                     AIYNTKFMINDSISFVSGSLSISLFNHLSKKTISSIELTTKDTLAQYFFNEKNNIIYL
                     ISKKGSIEGYRVNNFIIEPAIVFSATINLKENKFINLVKFYSDNNDLVISTTGKIADM
                     YRVNLSSSKIITLPSIKKFYDFIVAKNCFYFNNNNGTITKNNVKNLKVEKTYTLTNVN
                     DVAGFLQLSSNKKLLAAYYNETNIAVIDVEKEKLLYTIKAHDVIGMGLAFSNNCKYLA
                     TGGFDSKICLFNASTGEKLLNIFTPAGLDFVAADTTGYYMASKNSLEGLSFKLNENIY
                     PFEQFDLEFNRPDKVLAKSGLGDTTLIAAYKKAVSKRLKKNNSSSINLSQIEQLPSLS
                     ITDNTDVSYFTNLNFTELTINCYHEKQPIKALHVLVNNNPVYGFAGKNMGNQSHDTSI
                     TIKIPLAVGKNFIKLFCTNSSGQNSLQQTFEVRCNSNADTSAKTWFIGIGVSNYKDKD
                     MNLHYAAKDVRDLATTFTKDKNNVVVDTLIDSKVTIENILTLRKKLLTANPNDKVIIA
                     ITGHGMLDKNYDFYYGTYDIDFNNPNKRGLKYEMLEKILDSVPAQKKLLLIDACHSGA
                     LDKEELKKDSSKLFVDNTDTNKTTVVTRSTIKKKKLSKVSLNNTFELMQNLFSDFTNN
                     NGSVVISAAGGLEFAFESATWNNGVFTYCIRKALENFEADEEGDFNSKTSISELMKYV
                     SENVTRLTNGQQKPTSRREVLDFDWEL"
     gene            complement(28425..31253)
                     /gene="uvrA"
                     /locus_tag="PLU36_00420"
     CDS             complement(28425..31253)
                     /gene="uvrA"
                     /locus_tag="PLU36_00420"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HMZ45243.1"
                     /translation="MIVQGARENNLKNIDIAIPKNKLVVFTGVSGSGKSSLAFDTIYN
                     EGQRRYMESFGAYARQFLGNMERPDVDKISGLSPVISIEQKTTNKNPRSTVGTVTELY
                     DFLRLLFARIGEAYSYNTGKKMVKLSEEEIIQTIFEKFKEEKILILAPLVRGRKGHYR
                     ELFEETRKKGFLKVRIDGEVKDLTPKLQVDRYKVHNIEIVVDRLEVTMNMKLRLSQSI
                     QQSLKIGKDLIFVASANQISNNKDAVFSKQLMCVDTGISYEEPSPNTFSFNSPYGACP
                     TCKGLGVEYNINIDAVLPNKSKTIKEGGIVPLGEERDASVFKQVVDFAKKNKISLDTP
                     LEKLSDQQLNLILFGDKNITQDLVINMNDENIPNEYTGSYEGIIPMLKRWFTSTNSTD
                     FLQGWVEKFMTQGICSSCNGKKLRKESLWIKINNKNISDISEMNLDKLMLWLNTIEHN
                     LNSKQNIIAKDILKEIRERLQFLLDVGLSYLSLNRSSKSLSGGEAQRIRLATQIGSKL
                     QGITYILDEPSIGLHQRDNHKLIDSLKNLRDIGNSILVVEHDKDIMLASDYLVDIGPK
                     AGKHGGQIVAFGTPKEILKSNSDTAQYLSGKKAISIPNVRRKGNGKFIELIGASGNNL
                     KTITTKLPLGNLIVITGVSGSGKSTLINETLYPILSKHCYKSNIPIKEFKSINGLENI
                     DKVIEIDQSPIGRTPRSNPATYCGFFTEIRTLFASVPEAKIRGYTAGRFSFNVKTGRC
                     EVCEGGGMKVIEMNFLPDVYVHCEKCNGKRYNRETLEIRYKGKSISDVLNMTVDEACE
                     FFNAVPWLYRKIKVLQDVGLGYLTLGQSAVTLSGGEAQRVKLSTELGKKDTGKTFYIL
                     DEPTTGLHFQDIQLLMEVVNKLVDRGNTVLIIEHNLDVIKLADYIIDLGPEGGDGGGE
                     ILFEGTPEEMILNKESYTAKFLKQELSN"
BASE COUNT        11098 a         4780 c         4586 g        10812 t
ORIGIN      
        1 catcgcctgt ggcagttgtt atcaaatata gaaagaacaa agtttgaaag caaatcacaa
       61 ccagttgtta ttaatacaag caccttgccc agttgttatc aaatatagaa agaacaaagt
      121 ttgaaagcaa atcacaacga agaataaaat gggataaggg tgtgcctggt tgttatcaaa
      181 tatagaaaga acaaagtttg aaagcaaatc acaacataat aacatcacta ctttctttta
      241 ttgtgttgtt atcaaatata gaaagaacaa agtttgaaag caaatcacaa caaaatgtac
      301 taatcattcc gggaaaggta agttgttatc aaatatagaa agaacaaagt ttgaaagcaa
      361 atcacaacta atctgttatt ctataattac cgatagccgt tgttatcaaa tatagaaaga
      421 acaaagtttg aaagcaaatc acaactataa atagatttta tatttttaaa ttttgttgtt
      481 atcaaatata gaaagaacaa agtttgaaag caaatcacaa catagcagtt ttattattaa
      541 tctcttccac cgttgttatc aaatatagaa agaacaaagt ttgaaagcaa atcacaacct
      601 ctcaactttt atctgaaaat ctgttatcgt tgttatcaaa tatagaaaga acaaagtttg
      661 aaagcaaatc acaaccggta atcctacatt acttaaaaat ggtacgttgt tatcaaatat
      721 agaaagaaca aagtttgaaa gcaaatcaca acttgtttat ttcctcaacc cattcagttt
      781 ttgttgttat caaatataga aagaacaaag tttgaaagca aatcacaacg tatgcaagaa
      841 ggattaatct gattattgtg ttgttatcaa aattagaaag aacaaagttt gaaagcaaat
      901 cacaacttta attttattgt actcgtataa aatttaatta cctggttttt ctataagccc
      961 ttcctttttt ttcttaggaa cttttctaat tggatttata tacttgttgc cgccagttaa
     1021 tattttttcg tttttaagtt ttgtttttac ggcatcatct aaaacctttt tttgttgtgg
     1081 tgtcatattt actgttagaa ttaattgcaa gttattaata taagacgact caatacagcc
     1141 aatttttatt ttatttatgg tttaaataaa ttgatgtaat atacctgact tgcttagatt
     1201 tcggtaggtt ttaaaaaata attataaaaa ctatcttccc atataaacca ttaatatttg
     1261 tatatcgctt gggtttactc cacttattct gcttgcttga cccagtgttc ttggttttat
     1321 ttttttaaac ttttgtaagg cttcgttaga tagggcaatt actttattat aatcaaatgt
     1381 atctggtatt atcaattctt ccatttggct cattctatct actaaatctt tttctttttc
     1441 tatatatcgc tgatatttta cttgtagctc tgcttgttct ataatatctt ctttttcatt
     1501 gctatattta ttttttaata ttaaagaaaa gtttaatagt tcatttaagt ttactcctgg
     1561 tcttagtata attttagaag ctttttgttt ttcttgcatt acagccgagc caattttttc
     1621 aaaatattca tttatttctg ctggttctat ataaacatca ttcaagctgg ccttaatttc
     1681 ttctatttta ttttttttat agctcacttt ttccattctt tctaaagagg ctaaaccaag
     1741 tttaaatgcc atttctgtta atcttaaatc ggcattatct tgccttaaaa gtgttctata
     1801 ttctgcccta ctggtaaaca ttctgtaagg ttcattagtt cctttactta ttaaatcatc
     1861 tattaaaaca ccaatatatg catcgcttct ttttaaaatt aaaggttcta aattatttac
     1921 tttttggtgt gcgtttatac cagccattaa accttggcag gcagcttctt cgtatcctgt
     1981 tgtaccattt atttgccctg caaagaataa gttatgtatt atttttgttt ctaatgtatg
     2041 gtttagttgt gttggcggat aatagtcgta ttctatagca tagcctggtc taaaaatttt
     2101 acaattttca aaccctggta ccattttaag ggctttcact tgtacatctt ctggcaatga
     2161 ggttgaaaag ccgtttacat atatttctac agtattccaa ccttctggtt ctacaaatag
     2221 ttgatgacga tctctttctg caaaacgatt tattttatct tcaatacttg ggcaatatct
     2281 tgggccaacg ccttcgattc taccttggta cattgggctt ctatcaaatc ctgtttttaa
     2341 aatttcatga acagtactat ttgtataagt tatatgacat ggtttttgtt gctgcggttg
     2401 tattctttct atttctaaaa aagaaaatcc ggaaatttta tcatctccat gttgtacttc
     2461 cattttagag tagtctaaac ttctaccatc aactcttgga ggagttcctg tttttagcct
     2521 atcgctttca aatccaactt ctactaattg ttctgtaatg ccagatgctg atttttctga
     2581 aattcttccg ccacctaatt gcttttctcc aatatgaata actccattta aaaaggtacc
     2641 acttgtaatt attacagatt ttgcataaat ttcatgtccc agatttgtta ctacgccata
     2701 aacagtacca ttttttatga ttaaactttt aaccatatcc tgataaaaat caacattggg
     2761 tgtttgttct aacatttctc tccaggttaa ggcaaaaacc attctatcgt tttgggttct
     2821 tgggctccac attgctggcc ccttgcttct gtttaacatt ctaaactgaa tcatgctttt
     2881 atcagaaaca ataccactat atcctcccat ggcatctacc tctcttaata tttgtccctt
     2941 ggctatacca cccattgcag ggttacaact catttgtgca agggtttgca tattcatggt
     3001 tattaataat accttgcttc ccatatttgc agcagctgct gctgcctcgc aacctgcatg
     3061 acctgcacct gcaactatta catcataatt taaaaacatt cggcaaatat aataacagta
     3121 tgtttagcat tacataaagt atatggctta aaaccataag tatattggtt ggcgtacttg
     3181 tttcacgtgg agcatgtttt attttgtttc acgtgaaact acatttgact gttaaacatg
     3241 ttaaggtaaa aagtctcctt ctctctcata aggtcaatag attttttaga tttgtcttta
     3301 taaccacata aatgtaatgc accatgaaaa acaacccttc taatttcatt gttaaaactt
     3361 gtattaaatt cataagcatt ttcttttacc ctatctatac taatataagc ttctgaaata
     3421 ataggttgtt tgggtgaaga caaacaaaag gtaataatat cggtataata atggtgattt
     3481 aaaaattgct gattaatttt taataaatac tcatcgctac aaaaaatata atttattgtt
     3541 tctaactcta tcttttctaa ctcaaaaatg gtagcaacaa attgcttcag atttgtttta
     3601 tttttataaa ccagatttct atctgcatta ttaaaatata ctttactttt cattagtgta
     3661 aatatttctc aaacttcgta tttaaacttt ataaaattta taaaagagag gaaaatttaa
     3721 tataaattaa gttataacat atatatgcct gtaaataaag tgtttatgtg aaaataatga
     3781 aaatttaggt atttattcaa tgaatctaaa atctggttgg ttttaaataa aaaattattt
     3841 ataaataaaa atgctttgtt tctttgcgaa tgatttcgga aaaaataata attataactg
     3901 caccatctgg tgctggtaaa acatctatca caaaatattt attaaataaa tatagcaacc
     3961 ttagtttttc tatttctgca gcaacaagac tacccagaaa gggagaagtt gatggaaaag
     4021 attattactt tatttctacc gaggatttta aaaataaaat taaaaacaat gcttttgccg
     4081 aatgggagat ggtgtatgag ggcaaatatt atggtaccct aaaatctgaa ttagaaaata
     4141 tttggaaggc aaataaaata cccgttttag atattgatgt aaaaggtgcc attcatatac
     4201 aacaggctta tttaaacaaa accctttcgt tatttataca accaccaagt attgctatat
     4261 taaaagaaag attagaaagc agaggaactg agacagaaga aacgatacaa accagaatta
     4321 ataaagctgc ttatgaaatt tctttttgtc attcttttga taaaataatt gtaaatgata
     4381 atttagaaac tgcttgtaaa gaagctgaaa agattataga aactttttta tgtcaatcct
     4441 gagcctcctt ttaattcatc ttatcctttt ttgtcatcct aagcgtagcg aaggatctgc
     4501 tgaataacag caacaaagaa cagagagatt cctcgtaaac tcggattgac aaaatgcata
     4561 gattcctcgc aggctcggaa tgacaaatct tattttgttt cacttcgctt acagcatgtc
     4621 atcgttttgc cttttttgtc atcctgagcg tagcgaagga tctccagtag cacaatggtt
     4681 caaacagatg tttcacttac gttcaacatg acaacaaaaa cagatagatt cctcctaaac
     4741 tcggaatgac aatgcttttt tgttttattt tcattgacta catttcatac tgagccccat
     4801 tttatttcat cttatccttt ttttgtcatc ctgagcgaag cgaaggatct actgaaaagc
     4861 agcaccaaag aacaaagaga ttcctcgcaa gctcggaatg acaaagcata tttagtttca
     4921 ttttatttgt attgacaggc ttttaaagaa taattcaata aaaactcagt agtagattaa
     4981 ttttaacttc ggtaataaag gggatattcc aaagaataaa aaaaaggagc taaatattag
     5041 ctcccttata taaaatattc tataattaat atactttaaa cctataatct tttatagtag
     5101 ctgcattgcg taaagcttgg gcacttctaa aagcagcatt acgcatttgt cctaaaatat
     5161 tttgtttaat agctgcatca tcttgtactg gcggtatagt accaattgta ataactttta
     5221 cagcaaccac acctgcatta cttgccattg gtggtgttac tttaccaacc atatctttat
     5281 taaatgaaga gccagtaact tttaaatcgt accccatgtt tgcaattaca gggttagaaa
     5341 aataaacact atctgatgat tgaatagatg taccagatga gcttgcatac tcttctaagg
     5401 ttgagccctt aaatttagac tcaataatta gttttgcttt tttctcgttt cgtacaaatg
     5461 gttctaccat tggccttgca gatgctacag attttaagcc tactttatta actcctgtta
     5521 aaatagatac tacaaagttt tcgccaattt ccattggttc acttactgca tctactttct
     5581 tttcatataa ccatctaact aaatttctaa cagatgcctg ttgaaaatta ggaattgtaa
     5641 aatcgttttc ttttacatcc tgagcaatca taactgattt atttagcttt aatgcttctt
     5701 cttcaaactg ttttttattt ttaatttttg cagcaaattg taatgcagca gaattggctg
     5761 cattcatggt ttcgttgctt gctaagattg gttttgctaa atatgcaact ttaactgcat
     5821 cgctaaaatt cttttggctt atcacctcta tataatggta gccagagtat gcatcatttt
     5881 ctacttttac tattttttta tcgccagcct taccataaaa tattgtttct gcaaactctt
     5941 tgcttatacc agaaaattgt tgtaaagcaa aatcgtattc tccttttgtt gctttacttc
     6001 caccatcatc gctatatttt tgaactaact gattaaaatc tgctcctgac tttactaaag
     6061 cttctacact atcaattctt tttttagcaa tactatcttc taaaacgggg tttcttctat
     6121 cttctgtttt aactaaaata tgtctaactt ttacactatc tggtaaattt cttttgccta
     6181 tcattttagc aattaccaag tttcctccat ctaaatatgg cccaaacgta ctaccatctg
     6241 ctaacgcttt tatactatca gcatttggca tcttcatatc ttttttaggt aaaaaggcat
     6301 ttaaataagg aatttctgta ccaattcttc ctaaataagc ttctgcattc tcttcttttg
     6361 caaattctgg ttttaagctt aatatattat ttaatgtgtt taaagaatct atagcagatg
     6421 gggctgtact aaatactaca taagaaaaac ttcttccttc ttctttttgt tcaaaccctg
     6481 ctgggtgttt tttaatataa gctgcaattt cattatctga aatatcttta actgtactat
     6541 cagaaatact agtatatggt acaaaaacat aattaatttt tgctaaaccg ctgttatctg
     6601 cctttgtttt ttctactaac catgttggca catatacact ttgttgtaat atagcatcgt
     6661 actttgcttt taaagcttgt tgtttaattg gctctacata ggtttgaata acgccttgca
     6721 aattatcatt attcttagag ttttttaatt gagcaaatgc ctggcgtgct tcttctgctt
     6781 taaagtttcc attatcatct acaaattgtt tttctctttt taatggtgag ttttctccaa
     6841 ataaaatttc tccaacttct ttaccagtaa cggttatacc taatttttca cattcttgtt
     6901 ctaacaaaat tctttctact tccatattcc ataattgagg taaaatagag tttctatctt
     6961 gcccaccttg tgaatacata tttaacttct gatcaaaatc acctaactca atagcagttc
     7021 cattaacctt acctattgtt gatgttgctg tcataccagc accacctctt ccaacaccat
     7081 ccattaatat aaatgcaata agagctactg caataaagcc aaaagttaac catgcacctt
     7141 tttctctgat attttgaata atagacataa ttagtatata aaattttaag gaacgcaaaa
     7201 ataggggaat atacttattg aacaaattgt ggataaacaa tcaaaacctt tatttggctt
     7261 gatttataaa ttttaaataa aatagctttt agtttagata aaaattgaga agaaaatgct
     7321 taaaatattt attaagataa gtttgttggt aaattgaatt tggtgtgaat atttaataaa
     7381 aaaaattaaa aacactgttg aaagaattag aataaataca agaaagtttt aagattatag
     7441 gaaaacatgt tttgttttaa ctttttactc tatcttattt aacagttaat aaatggttta
     7501 tttgagattg tgaataatag aataattttt attaagtact gtttaaaaaa tacaaaaagg
     7561 catagcacta ttatatttta cattgttaat ataaggttag taatagtgta taaccaatct
     7621 attaattaac tttgctactt gttaataaaa aacttatccc aatattaaca gctataatag
     7681 taatagttgt ttatataaat atatactatt aaatattatt acaatgaata ttaacatagc
     7741 aaaagaacaa gttgccgaaa aaggattttt agatattgaa gtttcgccaa ctttagattt
     7801 atttgcagag atagaaaaat taaaaaaaga aaaaaatata gttttattag cacactatta
     7861 tcaagaacca tatatacaag atgtagcaga ttatattggc gatagtttag ggcttgcaca
     7921 acaggcagca caaacaaatg cagatattat tgtttttgct ggtgtacatt ttatggcaga
     7981 aacagctaaa attttaagtc caagtaaaaa agtattgtta cccgatttaa atgcaggctg
     8041 ttctttagca gatagtgcac caccaaaatt atttaaaagt tttaaagaca aacacccaga
     8101 ccatatagta gtaacttatg taaattgtag tgccgaaata aaagctttaa gtgatataat
     8161 ttgtacctcg agtaatgcag aaaaaattat agaaagtatt ccaaaagagc agccaataat
     8221 ttttgcacca gatagaaatt taggtgcttt tttaaacaaa aagaccggca gaaatatggt
     8281 tttatggaat gggtcttgca tagtacacga aatatttagc ttagaaaaaa taaccaaact
     8341 taaaattcgt catccaaaag ccaaatttat agcccatcca gaatgtgaag aagcagtttt
     8401 aaatgttgct gattttattg gtagtacatc tcaattatta agctataccg aaaaaaatga
     8461 agcaacagaa tttattgttg caacagaagc tggaatttta tatcaaatgc aacaaaatag
     8521 cccacaaaaa acatttatac cagcaccacc aaataattct tgtgcttgta acgattgtcc
     8581 ttatatgaag ctaaatacat tagaaaagtt gtatttatgt atgcaatatg aaaccccaga
     8641 aataactatt gatgaaaaat taaggttaca agcattaaaa tctttagata gaatgatgga
     8701 aattagtaaa gcagcaggtt taataaaata attaattaaa aagattcttt aattatattt
     8761 tctggattaa tatttagttt aattaaaaaa ccttctacag cttgcatcat tggcggaggg
     8821 ccacacaaat aaaaaatatt atttgaagca gtatattttt ctaacatggt tttattaata
     8881 aaaccatgtt cataattatc taatttttca tcggataaaa tattgacaaa gttttctttt
     8941 aataaatttt taaagaaagg ttctaaaata atatctgcct ttgttttatt tgcaaacaat
     9001 aaaatattat tagcaatttt attttgttgt tgtaagtgtt ttaaaatact caaaaaagga
     9061 gtaataccag caccaccagc aataaaaaca ccattgcctt tataagaaat atcgccaaaa
     9121 acatcaccaa taattaattc atcgcctaca cttaaaaata aaagttcgtt tgtaacacca
     9181 ttatgttgtg ggtatgtttt tataataaat tcaacaaaat tatctgtagg taaagacgta
     9241 aaagtaaaag ctctaatttt atctttccaa tttggtttat ttatagaaac atcaacagcc
     9301 tgacctgggt taaaatgtaa attttcaggt ttttcaacta taagtttcag aacatcctgt
     9361 gtgaggagtt ctaaagaaat aattttacaa atttgtgcca tgctttgtta ttttaaattg
     9421 tttattttac tcattactct attcacaata gcattacaat aaattaaatt aaattcaaaa
     9481 agttcggctt tactgtaaac cttttctacc tgcgacctta acatagattt tgccctattt
     9541 agcctcactt ttacatttgt ttcagaaata tttaaaatat cggcagtttc gttaatatta
     9601 aaaccattta tttctcttaa agaaaaaacc attctataat gtagtggtaa attagttaat
     9661 gcattttcta gaatatgact taactcttta ttttcataat ttaatttttt agtttccata
     9721 ttaaaaatgg gtttatcgtt ttcattaatt tctttcatac tacttttttt aaaatttaat
     9781 tttccttttt tatgaaaaca attatggagc ataattttaa ttaaccaaga cttaaagcta
     9841 gacttgccag aaaactgttg taagttttta taagcatcta taaaactttc ttgcattaaa
     9901 tcttgagtat cttcatgggt ataattataa cttctaccaa ttttatacaa ataagggtta
     9961 taacgcctaa caattatttc gaataaatgt ttattgccat ttaagatgct atcaataatt
    10021 tcaatatctg ataatttaat taattcttcc attttaataa atactaaata agtagagtaa
    10081 tcatctaacc aaaaagttac aaaaaagaat tgaaaaaggt aatttttttt tatttcataa
    10141 aaataaaaaa cagccatcac aaaacagtac atttgcagct atgaaaaatc ctgtaatttg
    10201 tattggtgca gcattggtag atgagttgta cagtgccaca agacccatgt taatgtcaac
    10261 caccaacgat gcttttgtac atagaagtgc aggaggtgta gcacgtaata ttgcccatca
    10321 attagcatta ttaaatgtac ctgtacagct tataagtgta tttggaaatg atggagaggg
    10381 agattggctt aaaacaattt gtagctatgt taatattaaa ttagatgcag ccataaccaa
    10441 tcaggcatta acaggtaaat acacaggtat tttaaattac gatcgttctc tttttactgc
    10501 attattaacc aacacatcat tagatgctat aacaccacag catttacaac agcacgaaag
    10561 tttattatct acagcatttt gtatagttgc agatgccaat attcatccag atactgtggc
    10621 atggttagct tttttctgtt ttaaaaaaaa tataccattt ataattgaac ctgtttcggt
    10681 accacctgca caaaagtttg ctaaaatgga attaaaaggt attcaaatgc taacacctaa
    10741 tgaagatgaa ttaccagcta tgtgtagtgg cggcggaaca acttttgaag aaaatgctat
    10801 agaactttta aacagagggg tagaaaaagt ttggctacat agaggaaaaa gaggctctat
    10861 gctttttaca aaagaaaata cttattcttt agaagcacca cctgtagatg atgcagatat
    10921 aaaagatgta acaggagcag gtgatggcag tttaagtggt tggctaatgg gtaaatattt
    10981 aggaaaagat gatgaagatt gtttaaaaat atcgcacaca ttaagtgccg aaattttaca
    11041 agtaaatggt gccattgcaa cacatcttga ccaggaaaaa ctactttctt tagtaaccaa
    11101 atattatcag caaagaaatt ttttatagct atataaaaga aaaaaataga taaacatttg
    11161 ggtgcaaaaa ataatgtata tttttaccca atgaccgaaa aaaagctatt tttattagat
    11221 gcatatgcat taatttttag agcatactat gccttaattc gtaatccacg tattacaagc
    11281 aacggaaaaa ataccaatgc ccaatttggt tttaccaata ctttagttga attaataaat
    11341 aagcaaaaac caacacatat tgctgtttgt tttgatgttg gtggttcggt aataagagaa
    11401 acagaatatg ccgactataa ggcaaacagg caagaaacgc cagaagattt attggcagcc
    11461 gtaccagata taaaaaagat aattgaaggg tttaatatac ctgtaatagg catagaagga
    11521 tacgaagcag atgatgtaat tggtaccttg gcatgggagg ctcacgacgc aggcttccaa
    11581 gtttttatgg ttacgccaga taaagattat gggcaattgg taagagatgg aataagtatt
    11641 tataaaccag ccacacaagg taatgatgtt gaaattttag gtccaaaaca agtatgtgaa
    11701 aaatgggata tagaaagagt agatcaggta atagatattt taggattaat gggagatgca
    11761 gtagataata ttccaggaat taaaggtgtt ggagaaaaaa ctgcagcaaa gcttttaaaa
    11821 gaatatgaca atttagaaaa cgttttagcc aatgctgaaa atattaaagg tgcattagga
    11881 gaaaaagtaa aagcaggtaa agcagatgct attttaagta aaaaattagc aaccataatt
    11941 actaatgtgc cagttagttt taatgaagaa gagtttaaat taaaagaatg gaataaagaa
    12001 gcattaaatg aaatttttgc agaattagaa tttagaactt taggaaaaag attattagga
    12061 gaagatttta ctattgcttc aaacaatgtt caaacagatt tgtttggaaa tattgtagaa
    12121 agcaaaactc ctaaatcaga aaaaaaacag aaagaaacaa ctaaagaaca agaagagttt
    12181 aatttaatta atacaacagt agatgaagaa aataaagcat ttaaaaatat aaacaataca
    12241 acacacaatt atattttgat agatagtgag gagaaaatag ataaccttat taaacaacta
    12301 ctacaacaaa ccgaaatttg ttttgataca gaaacaacca atattgatgc aaataatgca
    12361 gaactggtgg gcataagttt ttcatataaa ccacacgaag cattttacat tccattatct
    12421 caaaacttta cacaagcaca acaaattctt gaaaaattta taccgctatt taataacaca
    12481 aatattgttt ggataggaca aaatattaag tacgatttat tagttttaaa atggtataat
    12541 attcaaatac aaggcaatgt gtttgataca atgttggcac attatgtagt agaaccagag
    12601 ggcaaaagaa gcatggatat tttaagtgaa cagtttttac aatatcaacc aatacctata
    12661 gaagatttga ttggcaagaa gggaaaaacg cagggcaata tgagagatat tgaagtagaa
    12721 aaaataaaag aatatgcagc agaagatgca gatattacct tacaattaaa acaaaaaatt
    12781 gaaccattat taaaagaaaa agaagtagaa aaagtttttt acgaagtaga aaatccatta
    12841 gttaaagtgc ttacagcaat ggagtttgag ggaataaaaa tagatgaagc ctttttaaaa
    12901 gaatatagta aagaattaag taaagaagca aaagcagcag aagaggcggt atataaaaaa
    12961 tcggaagtta gatttaattt agcatcaccc aaacaattag gagaagtttt atttgataaa
    13021 ttaaaattag atagctcagc aaaaaaaaca aaaacaggtc agtatcaaac cggcgaagat
    13081 attttacaaa aactatcggc aaaaggccac tctattgcag aagatatttt agtgtataga
    13141 gaattaacta agcttaaatc aacctatgta gatgctttac cagaaataat aaattcaaaa
    13201 acaggaagag tgcatactac ttatggacaa gcagttgctg ttacaggcag gttggcaagc
    13261 aataacccca atcttcaaaa tattcctata agaacagcaa aaggaagaga aataagaaaa
    13321 gcatttatac caagagatac caatagagtt ttaattagtg cagactactc tcaaatagaa
    13381 ttaagaattg ttgcagccat aagtaacgat gaaaatatgt gtgcagcttt tagagcagga
    13441 aaagatatac atacagcaac cgctgcaaag gtttataatg tggcagaaaa tgaagtaacc
    13501 aaagaaatgc gttacaaagc aaagagtgta aattttggaa ttatttatgg acaaggagct
    13561 tttggcttag cagataattt gggtattagc agaacagagg caaaagaaat aattgacaat
    13621 tataaaagag aatttccggg cattcaaaaa tatatggacg atacaataaa ttttgcaaga
    13681 gaaaatggat ttgtacaaac cattatggga agaaaacgtt ggctgaaaga tataaactca
    13741 tctaatttta ctgtaagagg ttttgccgaa agaaatgcta ttaacagtcc aatacaaggc
    13801 acagcagccg atattattaa aatggcaatg attaaaatac atactgcttt acaaaaagaa
    13861 caactacaaa gtaaaatgat attacaagta cacgatgaat tggtttttga tgtattatcc
    13921 tctgaaatag aactaataaa gccattaata ttaaacaaca tgaaatctgc aatggtatta
    13981 cctaaccaag ttcctgtaga agcagaattg ggcataggaa ataattggtt agaagcccat
    14041 tagttattta aaacaatacc actcatcatt atttctttaa ttagcagtac catttattgt
    14101 ttatctagta tattgcccaa attcaaaaaa acaaagcctg gtaattttta aaatctaaat
    14161 aagcaggcaa ctttttatag cactttttat gaaactttct ttttctcttt tgttagtatt
    14221 ggctacaaaa actctaatgg ctcaagccca aaatacacaa gagctaaata atcaatcggc
    14281 ctctaataat ttacaaacaa tttaccataa aaccgttcag tataaacaaa atgtttttac
    14341 aggaattgag tattttagaa attatggaaa aataaatggc cacgcttttt ggaatgaaaa
    14401 tgaatttata aacggaaatg tgggctatga tggaaacatc tatccaaacc tgccattaaa
    14461 gttagattta atggtagaag agttgattac acatggggta aaccaaggtg tgcaaataaa
    14521 aataattaga gaaaaaattg attcttttac tataggatca gtacgttttt tgcacttaac
    14581 acccgataag aataaagtac cagatgggtt ttatgaaata ttacatagtc aaaaatatac
    14641 agtgttttgt aaaagaaata aattaataaa agaagttata gtacaaaacg aactggaaag
    14701 aaatatagaa gaagaagccg tttatttttt aaaaaatcag gataatataa ttgtaataaa
    14761 aaacagacaa atactatttg atttatttag agataaaaaa aatcaggtat tagattatat
    14821 gagcaatttg aatttgaatt ttagaaaaca aaaagaagct tttattgttg aatcaataaa
    14881 atattacgag caaataacca actaataaga tgaaattttc tttacgactt ttaacccttt
    14941 tggcattaat aattttattt agttacgcaa aagcacaaaa cgaaaataat attacgttag
    15001 atttaaaacg agccagcttt gccgaatttg tacagaacgt tgaaaaacaa acaaactatt
    15061 ttttttatta taataaagta gataccgata gtgtatttat cactatctct gttcaaaatg
    15121 ctactattgc cactgtttta aatactgttt ttgaaaatac agaattatat ttttctatag
    15181 cagataataa aaaaatattt gtttcaagaa acgttcaatt attaaccaat ttaggaacat
    15241 tgttttttaa aacaaacgag caagtaaaca aaaaagaaaa aacaattact gtaatagaag
    15301 actctataga agaaaccaat accaatgatg tagccataca aaataaatta attgttatag
    15361 gcaaaccaaa tgcagcagta agtggcaaag tattaataac cggccaggta aaaaatggaa
    15421 aaaataacga accaattgta ggtgctgtta ttgcagcagg taaaacaaat gttgtaacgg
    15481 attatttagg aaaatttaca attagcttaa acaagggcag aaatatgctt acagcatcta
    15541 gtataggaat gagagatgcc gtaagacaac tccttgttca atcatcaggc aatattaata
    15601 ttgaaatgca agaaaatatt actgccttaa aaggagtggt ggttactgca acaaaaagaa
    15661 acactggagt aaaaacaccc aaaatggggg ttgaaaaaat tactatcaaa caattaaaac
    15721 aacaacccgt tttatttgga gaagcagatg tgctaagagc ggttttaaca ctacctggtg
    15781 taacatctgt tggcgaagca agcacaggat ttaatgtaag aggcggatcg gttgatcaaa
    15841 atttaatttt atttaatgat gcaaccattt tcaatccttc tcatctcttc ggtttctttt
    15901 ctgcttttaa cccatcggtt gttaaaggag ttgaattata caaaggaagt attccagaaa
    15961 aatttggtgg tagaatttca tctgtattag atgtatctgt tcaagaggga aataaaaaga
    16021 aatttggtgg agcaggtggc attggccctt taacaagcca tttaatgttt gagggtccaa
    16081 taaaaaaaga taaaggttct tttatcattg gctttagaac aacctactcc gattggatat
    16141 taaaaaattt aaaagatgta agttttaaaa atagcaaagc cgcatttaat gatttaagta
    16201 ttcacttatc tcaacaaata aacactaaaa actttttgta cataacaggc tataccagta
    16261 aagataattt taggttagat aaagacacaa gttatgctta taaaaacaac aatataaata
    16321 taaagtggaa acacattttt aaaaataacc ttataagcaa tgtaactgct gggtatgata
    16381 attataacta tggtatagaa agttctttaa aacctctttc ggcatttaca ttaaaattca
    16441 atatcaatca atattactta aaggcagatt ttactaaatc ttatggcaat gtgcatcgta
    16501 taaactttgg ctataatggt aattttatga aattaaaccc aggaagctat gcaccaagtg
    16561 gaaaagtgtc tttagtgaat gcaataaata ttcaacaaga aaaaggaata gagaatgcaa
    16621 tttatttagg agataaaatg gaactatcct caaagcttgc agtagaagcg ggtattagag
    16681 tatctgcatt tactgctatt gggtcttcaa aagtatataa gtattataat ggtttagaaa
    16741 gagatgatgc aaacattcaa gatactattt tctataaaaa tggccaacca ataaaaacct
    16801 actggggccc tgaggtaaga ctttctacac gatataccat taacgaaaca agttctataa
    16861 aagctggctt tagtacgcaa cgccaatata ttcacatgtt atctaacaca actgctattt
    16921 caccaacaga tatttggaag ctaagcgata cttatataaa accacaaatt ggccaacagg
    16981 tttctttggg tttttataaa tcatttccaa aatcagatgt tgaagtatcg gttgaagtat
    17041 attataaatg gctacaaaat tttcttgatt ataaaagcgg agcattgcta ttgttaaacc
    17101 aacatattga aacagatgta ttaaactcta aaggcaaagc ttatggtgta gagtttatga
    17161 taaagaaaaa ctcaggaaaa ttaaatggat ggttaagtta tacatactct agaacaatgt
    17221 tacaaacaga tgagccaaca acaagttata aagtaaataa aggcagctat tatcctgcaa
    17281 actttgataa accacatagc ataaattttg tgggcaacta taagttctct cataggttta
    17341 atgtatcgtt aacaaccgtt tacagtacag gtaggccaat aacattaccc attgcaattt
    17401 ataattatgg tggtggagat agagtatatt attctgatag aaaccaatac agaattccgg
    17461 atttctttag ggcagatttt tctatgaaca tagagggaaa tcataaaatt aaaaaattag
    17521 cccacagctc atggtcctta ggtatataca atattacttc aagaaaaaat ccatattctg
    17581 tttatttcgc aacagaaaac ggaaaaataa aaggatatca attatccatt tttggttcag
    17641 caattccatt tataacgtac aattttaaat tttaaataaa ggctttacat aatgaaaagg
    17701 catataatat atttagtaac tatagtagca ataataactg gctgtagaga aaattataca
    17761 ccaccagaag taactacaga ccataaaata ttagtagtag aaggttttat agatgtgaat
    17821 aaagacagta gttattttaa attatcacgt actcaaaaat taggttctgt ttcatctgtt
    17881 aaaccagaag caggagctaa tatttcaatt ctttcttcca acggaacagt gctaacacaa
    17941 tttaaagaaa gagccaatgg ccaatatgtt gtatatggtg caacactatt gccaacagag
    18001 ttttataggt taaaaattac tacttctgga gcggttgttt accaatcgga tttaattaaa
    18061 ggaaaaataa cacctgcaat agatagtgta acttggaaaa ttgataacga taaaaatggt
    18121 attcagtttt atgtaaactc tcacgatgct gctaatgaaa caaaatatta taaatgggat
    18181 tgtgaagagg tttgggaata tagggcttac tatgaatcgc agtataaata tttgggagat
    18241 actattattg aaccaagatt accaggagaa caaatttaca gatgttggag aacggttttg
    18301 ccaacgcaaa tttttattaa tacaaccgaa aaattatcat cggatgtaat aagtaatgaa
    18361 aaaattttat ttgtaggcaa aggatctaat aggtttgatc aacgctacag cattttggta
    18421 aaacaaagag cattaaccaa agaggctttt gagtattggc aacaattaaa acaatctagc
    18481 gaattaggag gctctgtttt tgatgtgcaa ccaacacaac ttttaggcaa tttgcattgc
    18541 gttacaaacc ccaatgaacc tataattgga tatgtgagtg caaccaccac tgcagaaaaa
    18601 agaatttata ttttaaatac caatgttcca acttttgtgc aaccatatcc tgcaacttgc
    18661 gaatttgctg ttgttgcaaa taataaagac agcatgaaag tgtattatac caataaaaga
    18721 tatataccca ttgcaccaga tatgcaagga gcttatttag caggctctac acaatgtaca
    18781 gattgtagag ttttaggtgg cacaacaatt aaaccagcat tttggtaaaa acattaaagc
    18841 agaaaaatta tattttaaga aagttaatta tgcaaaagct tacattttcg gtattactat
    18901 ttttagcgtt tagttttaaa ctatttgcac aggttgatga tagaaaagat gtttcattaa
    18961 aatttaatga gtacgtttta aataattttc aagaaaaaat atttgtacat actgatagaa
    19021 atttttattt atctggcgat ataatttggt ttaaaatata caatacatct gcttctcaaa
    19081 atacatttac taccattagt aaaattgcct atgtagagtt attaaatact gaaaacaaat
    19141 ctttacttca agcaaaaatt gctttaacag atggcactgg taatggacaa tttgttttac
    19201 cattatcctt accaacaggc aattatattt ttagagcata taccaattgg atgaaaaatt
    19261 ataacgaaga aattatgttt gaaaaaacca tatctgtaat taatgctttt gaaaaaaatt
    19321 ctattgatac agcaaaaaaa gcaattagtt acgatgtgca attttttcct gaagggggaa
    19381 acctggtaaa taacatacaa agtaaaattg catttaaagt tataaacagc aatggctttg
    19441 gggtaatgac aaaaggtaaa atagtaaacc aaaacaacga aaccatacaa gagtttaata
    19501 ctaaccattt aggtattgga tcgtttttat ttacaccagt aaatactaat acatataagg
    19561 ctttagtgtt tacccctacc aacgatactt taagttataa cctacccaaa attttaaatg
    19621 atggttatgt aatgcaagta accagaaatt caacacatac aaccattact gtatttgcca
    19681 gtaattattt gaataataaa gcagttaaat tagttataca taacaacaac actatacaat
    19741 ataaccaaac acaaagtata aataatcaac aagcagtttt tacagtacat aataatttat
    19801 tgaaagaggg aattaaccat cttactattt ttaatgatta cgacttgcca gtaactgaaa
    19861 gattattgta taaaagacca accgaaaaag caactttaaa aacgaacatt aacaatactg
    19921 tatttaacaa cagagaaaaa gtaaatataa tggttaatgg ggtaaccaac actaatcaac
    19981 caatagaaac taatctttct gtagcggttt ttaaaacaga ttcttttcaa ttaaataatg
    20041 atgtagatat tcagcaatac ttatggctta catctgaact taaaggaaat atagaagaac
    20101 ctgcatacta ttttaacact atcaatgaag aaacagatag tgcattagac aatttaattt
    20161 taacacaagg ctggagaaga tttaattggc taaatatttt acaatcacca acaacaccaa
    20221 aatatttacc agaatatgaa aatcatatta taacaggtaa agtaatagat aaaagaacat
    20281 tacaaccagc agaaggcata ggaatttatt taactattcc tggtaaagag tttgggtttg
    20341 cagcagcaca aacaaatagt gaagggaatt tttttacatt aatgccaacc atttataaca
    20401 gttctgaatt aatagcacaa accaataata gaactgatag caattataga attgatatta
    20461 atagtgctta ctgggaaaaa actactggct ataaaccaac aaaattttta atttcaaaaa
    20521 acaacgaaag actactgaat aattatggca taagtgtaca ggcaaattat atttatcact
    20581 ccaaaccatt atataaagaa acacaggcct atagcccatt ttatggaaag gcagatgaag
    20641 tgtatttttt agataactac accagatttt ctacaatgga agaggtaatg agagaatatg
    20701 ttacaggcgt atctgtacga aaaagagcag gtaaatttat ttttcatacc gttgatgcac
    20761 caagatttca gttttttact acagagcctt tgatattatt agatggtatt gctgtttttg
    20821 atgcagataa aattattgct accgatccat taaaacttaa aaaagtagaa gtaacaacaa
    20881 gaaaatttta ttatggcagt tttgcctttg atggtattac aagctttaca agttatgatg
    20941 gaaagtttgg aaaaaatgat ttacatactg aatccttagt gatagactat gatgcacttc
    21001 aacaagaaag agaattttat gcacctaaat atgatatagt agaaaataaa gaaagcaggg
    21061 tgccagatta tagagtattg ctacattggt ctggcactat taaaacaaca acaaatgggg
    21121 caacagatat agaattttat acctctgatt taggtggaaa atatacagcc attattcagg
    21181 gtattaccaa tacaggcaac ccagtaaaat ctatggttga gtttacagta aagtaaacta
    21241 taaaaatttt gtagcattaa ttaaatcttc aggggtatca atttcaatac ccatgtaatc
    21301 ggtaacaacc atttttaatg ctataccatt ttctacatag cgtaaacact ctattttttc
    21361 agcagcctct aatggtgtaa tttcccactg tgtaaaatgt aataatgctt gtttggtaaa
    21421 agcatataca ccaatatgtt cataatatgt tggagcaata tttttatctc tgtgataagg
    21481 tataacagaa cggctaaaaa gcaaactatt catattttta tcaacagcaa ccttaacaaa
    21541 attaggatcg tttatttgtt gttcattttt taaaacctgc attaagcttg caacttgaac
    21601 agagctgtct tcaaaaacat ttaataattt ttctaaaggt tcttttttaa caaatggctc
    21661 atcgccctgc acatttacaa ttatatctac gtctatattt tttacagctt cagcaattct
    21721 atcgctacca ctttcgtgtt cttgcacact cataacagcc ctacctccat tatttacaat
    21781 ttcattataa ataatagcac tatctgtaac tacccaaacc tcgttaaaca agcctgtggc
    21841 aagtgtatta tcataagtgt gcctaataac tgttttgtta cccaacattt gcataagctt
    21901 tgcaggaaaa cgagtagcag cataacgtgc aggaataaat gcagaaattt tcataaatat
    21961 tttgtattaa cttcttttaa attattaaac acttaattct aaaaatatag gacagtggtc
    22021 gctatgttta atatctgcaa aaatacttgc agttttaatt ttatttttaa gagcatttgt
    22081 tacacaaata taatctatac gccaaccctt gttttgtaaa cgcacacttg gaaaacgctg
    22141 gctccaccat gtatatccac ctattgtatc agggtttttg aatctaaaac tatcaatcca
    22201 acctgtatgt aaaaattttg taagccaggc acgttcctct ggtaaaaaac cagatgaatt
    22261 tttattgcct ttagggtcgt gaatatctat ttcggtatgg gcaatattat aatctccaac
    22321 aagtacaatg tttttatgct ctttacccaa agcttgtaca taatcataca tttcatttaa
    22381 ccattggtat ttataggttt gcctttcatc cccactggtg ccacttggaa aatatgcatt
    22441 aattaaaata aagtttttga aataaagttg aattactcta ccctcaaaat cgctttgttc
    22501 tatttgtgta ccataaacaa ctttgtttgg ttttaatttt gtaaaaacag caacaccact
    22561 atatcccttt ttttgagcag aaaaaaaata ggcctcaaag cccaattcct gaaagggttg
    22621 taggtcaata tcacttatgg ttgccttcgt ttcttgcaaa caaataatat ctgcagggtt
    22681 agtttttagc caatctataa atcccttttt tattgcagcc cttataccat ttacattgta
    22741 tgaaataatt ctcattgtat aaatttaata gttagtttta atataaaatt taatataaat
    22801 taattaaggt attattattg agtataaaaa ttattaaata agaaatatac aaaaatgaaa
    22861 aacaaagata aaattatacc aagtagagaa cattattact tagaaggccc aaaacataga
    22921 gttaatgagc ttgtatgggt ttggagtatt tttgttgaat ttattggagc aataagaaaa
    22981 ttacatttta cgggtccctg tattactgtt tttggtagtg ccagatttaa agaagataat
    23041 gaatattaca ttgcagccag agagtttgga aaaagaattg cacaattagg ttttaccact
    23101 atgactggtg gaggaccagg aattatggaa gcagccaaca ggggtgcatt tgaaaatggt
    23161 ggatatagta ttggctgtaa tattaaacta ccttttgagc aaaaagaaaa tccatacata
    23221 aacaaatcgt acacgtttaa ttattttttt tctcgtaagg ttttacttgt aaaatattct
    23281 tatgctttta taataatgcc tggtgggttt ggtacaatgg atgaattgtt tgaaacctta
    23341 actctaattc aaacaaaaac aataaataat tttccggttg ttttatatgg taaaaaatat
    23401 tttcaacccc ttataaatta tattgaagaa atgatagagc aaaaaacaat atctaaagaa
    23461 gatttgaatt tacttttagt aaccgatgat tttgaggaag ctataaatca tataaaggcc
    23521 tatgtacata agcattataa ggtaaaacca cgcaaaaggc tttggtggtt attcgaaaaa
    23581 aaataaatgg tttatagttc ccaatcaaaa tctaaaacct ctctacgaga ggttggtttt
    23641 tgttgcccat tggttaacct ggttacattt tcactcacat atttcattag ctcgctaata
    23701 gaagttttac tattaaagtc tccttcttca tctgcttcaa aattttctaa agcttttctt
    23761 atacaataag taaaaacacc attattccat gttgcacttt caaaagcaaa ctctaaacca
    23821 cctgcagcag atattacaac acttccgttg ttgttggtaa aatcagaaaa aagattttgc
    23881 attaattcaa acgtgttgtt taaacttact ttactaagct ttttcttttt tattgtgctt
    23941 ctagttacca cagttgtttt atttgtatct gtattatcca caaataattt tgaagagtct
    24001 tttttaagtt cttctttatc taaagctcca ctatgacaag catctattag taataatttt
    24061 ttttgtgcag gaacgctgtc tagaattttt tctaacattt catattttaa acctcttttg
    24121 ttagggttat taaaatcaat atcgtaagta ccataataaa aatcatagtt tttatctaac
    24181 ataccatgac ctgtaatagc aataattact ttatcgtttg ggttggcagt taataatttt
    24241 tttctaaggg taagaatatt ttcaatggtt actttgctat ctattaaagt atctactact
    24301 acattatttt tatcttttgt aaaagtggtt gctaaatctc taacatcttt agctgcatag
    24361 tgcaggttca tatctttatc cttataatta gaaacaccaa tacctataaa ccatgtttta
    24421 gctgaggtat ctgcattgct gttacatctt acttcaaagg tttgctgtaa agagttttga
    24481 ccagaggaat tagtgcaaaa aagtttgata aaatttttgc ctaccgccaa aggaattttt
    24541 atggtaatag aagtatcgtg gctttggttg cccatatttt ttcctgcaaa accataaact
    24601 gggttattgt ttactaaaac atgcaatgcc tttataggtt gtttttcgtg atagcaatta
    24661 attgttaact cggtaaaatt taaattggta aagtagctta catctgtatt gtctgtaatg
    24721 cttaaagagg gtaattgctc aatttgcgat aggttaatac ttgaagagtt gtttttcttt
    24781 aatctttttg aaactgcttt tttgtatgct gcaattaatg tagtatcacc caaaccagac
    24841 tttgccaaca ccttatctgg cctattaaac tccaaatcaa actgttcaaa tggatatata
    24901 ttttcattta atttaaaact taatccctct aaagagtttt tacttgccat gtaatatcct
    24961 gttgtatctg cagctacaaa atctaaccca gcaggtgtaa aaatatttaa aagcttttct
    25021 cctgtgcttg cattaaataa gcaaatttta gaatcgaaac cacccgttgc aaggtacttg
    25081 caattattgc taaaagccaa acccattcca attacatcat gagccttaat agtgtaaagt
    25141 agcttttctt tttcaacatc tattactgca atatttgttt cgttataata agcagccaat
    25201 agttttttat tagaagataa ttgtaaaaag ccagcaacat catttacatt agttaatgta
    25261 taagtttttt ctacctttaa attttttaca ttattttttg ttatagtacc attattgtta
    25321 ttgaaataaa aacagttttt agcaacaata aaatcgtaaa atttttttat agacgggagt
    25381 gttataattt ttgaactaga taaatttact ctatacatat cagcaatttt acctgttgta
    25441 cttataacta aatcattatt atcactatag aattttacga ggttaataaa tttattttcc
    25501 tttaaattta ttgtagcaga aaatacaatg gcgggctcta taataaaatt attaacccta
    25561 tagccttcta tgctaccctt tttgctaatt aaataaataa tattattttt ttcgttgaaa
    25621 aaatattgtg ctaaagtgtc tttggttgtt aattcaatag atgaaatagt ttttttagat
    25681 aaatggttaa acaaagagat agaaaggctg ccacttacaa atgaaataga atcattaatc
    25741 atgaattttg tgttataaat agcctgtgct gcataaaaaa gttggtgtgt gctattgttg
    25801 gaaaaatcga attttactat tctttcccca acggtaaata gagcatcatc ttttgcacta
    25861 aactgtaagg ttatatttgg attggtatat tgtttgttta tttttaactc cacatttgta
    25921 ttaaaactgt gaatgttttt atatccacta atatcaaaga gttttgtttt acttatgtat
    25981 gggtaggttg caaaaattcc catttgatcg gtagtaaaat ttaatgtatt taaataagca
    26041 tatttatttg cagtttttgt tattgctttt aaatagcctg tgtttaggtc aactaataca
    26101 acactgtttt gttggttggc ataagtaagt aaactgccat ctggtgaaaa atcgggctcg
    26161 ttataaaact ttgtatcccc gtattgggca acagctattt tttctttaaa ataaaattta
    26221 tttttatata aattccaaac attaatagag ctatcgttgg cagtataaaa taaataatta
    26281 ccgttgtagc taactttcat atttaaaagc tttgcttttt ttctgtacca attcacatca
    26341 tgttttgttt gtaaattcca acgataaacc atactatccg taccactact aaataaaata
    26401 tcttcgtttc taccaacata gcaaagctgt gtaactgcac ctttgtgttt atttaaactg
    26461 tatagttttt cgccagttat aaaattccac acttttatta aagagtcata tccgccactt
    26521 gctatttctt tattattagg agaaaaagca atacagtatt cagtattaga ggtattaatt
    26581 gtagtaatta ctgcataatc aaatgtgcta taaattttaa tggttttatt atcactacag
    26641 gttgcaaaat aacggccatt ggggctgtat gctatggaat aaactatttc attttgtggt
    26701 tgaaaagtgt gaattgtttt tcctagatgt aaatcccaaa ttctgatagt attatttgca
    26761 tcgttggttg cataatattt gccagatgga tgcattgcaa cactatagct tgcaaactct
    26821 ccaagctgta cttctaaact tactttatta ttagtgtgta ttttttgtaa tgcctttttg
    26881 gttattgtta gaccttcttt gttattagat ttggtaaaaa acttttctgc cttttttaaa
    26941 gcagtatctg ccaaggaaac acttcctgtt ttgtattgaa acaaaccatt taaaaaactt
    27001 agccacccac caacatcaat atttttttca ctttcataca ctgccgcaaa actcattaaa
    27061 gcaattctaa catttagcaa atcgttttta tcaaaagcat ttagtgttag ggttacccat
    27121 ttttcaataa tattattctg tttaatttga ttctttaaag cataagcaat ttcggccctt
    27181 ttattttttt ctgtttgagc aatatagagt aaaagatcat cgttggccat tggggtatta
    27241 tttaaaagcc aggtagcata aatttcagaa aatgaaaaat tattgccaat atacttacct
    27301 gggtatgctt tcataaagtt tataaaagca actatatgtt tagtgttggt gctatcaaag
    27361 gctttaataa tattaacgcc tttaaaaatt ccttctgttg ctttttgggc taaagtatta
    27421 tttggtttta aagtatcttc tgcgtaagca ttttgaacat aaaataattc aacttttaag
    27481 ttttttatta gctcaaaatg aattttttca tcatcgtaat acaacaaaaa tctacggctt
    27541 aataatagtt tttcttttgt attatccaac aagtttattc caatatctaa caatggcaca
    27601 aacttatttt tttttgcact cacaggggtt tggcaataaa tattagccaa atctttaggc
    27661 tctattggta cagtagagga tgatttaata tatataatgg ttctgttatt ttcaacactt
    27721 acaactcttc ctatacctat agaataacta ttttttacat cttcttcatt tttatattta
    27781 tcatcgtatc ttttataaat atttactttg taatcgtcat ttgctatacc atcgtatttg
    27841 cctgcagcaa ctattatttt taagctgtca gagtttttaa aactatctat ttgaagaata
    27901 gggaaataaa tataactgcc aaaggtggtg gtcatttttg ggtaattaaa agtccagttt
    27961 ttttctagaa tagtattatg taagttattt aaaaatagaa tagcattgga ttcgggaata
    28021 atatgtttaa attttctagc attcaaaagt cccttgtaca tataataatg agcattatcc
    28081 ggctcttgta aatacatgta acaaatacct ttatttatat ttaaagagga gtatttatcg
    28141 tctgaatatt tattttgctt ttcagcaatt ttttctgata aattaaagta gtataatgca
    28201 gaatcatatt tttcagcaag tagcaagttg gtgccttttt caaaatgtgc tttaaaatca
    28261 atttctgaat ttgtttggct aaaaacaaag agatgaaaaa ttaaaaaagt agaaagtaaa
    28321 actaatttca tataaaacag gtaaacttat ttcataaaac aaaaagggat acagttacaa
    28381 aatacaattt ttccgaaata atggttgtta atatcagttt aaacttagtt tgatagctct
    28441 tgttttaaaa actttgctgt ataactttct ttattaagaa tcatttcttc gggtgtgcct
    28501 tcaaataata tttcacctcc accatcgcct ccttcaggcc ctaaatcaat aatataatct
    28561 gcaagtttta taacatctaa gttgtgttca ataattaaaa cagtattgcc tctatccacc
    28621 aatttgttta ccacttccat taacaattgt atatcttgaa aatgtaaccc agtagttggc
    28681 tcatcaagaa tataaaaagt tttgcctgtg tcttttttgc ctaattctgt agataatttt
    28741 acacgttgag cttcgccacc actaagtgtt acagcacttt gcccaagcgt taaatagccc
    28801 aagcctacat cttgtagaac ttttattttt ctgtaaagcc agggcactgc attaaaaaat
    28861 tcacaggctt catcaactgt catgtttagc acatcgctaa tgctttttcc tttatatctt
    28921 atttctaatg tttctctgtt atatctttta ccattacact tttcgcagtg tacataaaca
    28981 tctggtaaaa aattcatttc aatcactttc attccgccgc cttcacaaac ttcgcatctg
    29041 cctgttttta cattaaatga aaatctgcct gcagtataac ctcttatttt tgcttctggt
    29101 acactggcaa acaaagttct aatttccgta aaaaaaccac aataagtggc agggttgctt
    29161 cttggtgttc tgccaattgg gctttggtct atttcaatta ctttatctat attctctaaa
    29221 ccattaatac ttttaaattc cttaattgga atattacttt tataacaatg tttgcttaaa
    29281 attggataca gtgtttcatt aattaaagtg cttttaccac taccgcttac acctgttata
    29341 acaattaaat ttcctaatgg aagtttggtt gttatggttt ttaaattatt gccactagca
    29401 ccaattagtt caataaattt accattaccc ttccttctta cattgggaat agagatagcc
    29461 tttttgccag ataaatattg tgctgtatcg ctgttgcttt ttaatatttc tttaggtgtg
    29521 ccaaatgcta caatttgtcc tccatgctta ccagcctttg gtccaatatc taccaaataa
    29581 tcgctagcta acataatgtc tttatcatgt tctaccacta aaatgctatt accaatatct
    29641 cttaaatttt ttaaagaatc tattagttta tgattatctc gttgatgaag tccaatactt
    29701 ggttcgtcta aaatataggt tataccctgt agtttactac ctatttgtgt tgccaatcta
    29761 attcgctgag cttcgccacc actaaggctt ttactgcttc tgtttaacga taaatagctt
    29821 aggccaacat ctaataaaaa ttgcaatcgt tctcttattt cttttaaaat atcttttgca
    29881 attatgtttt gttttgaatt taaattatgt tcaatagtat taagccaaag cataagttta
    29941 tctaaattca tttcgctgat atcgcttatg tttttgttgt ttatttttat ccataaactt
    30001 tcttttctta atttttttcc attacaacta ctgcatatac cttgagtcat gaatttttct
    30061 acccaacctt gtaaaaaatc agtactgttg gtagatgtaa accatcgttt taacatagga
    30121 ataattcctt cgtaactgcc agtgtattcg ttaggaatat tttcatcgtt catgtttatt
    30181 actaaatcct gtgtaatatt tttatcgcca aagagtatta ggtttagttg ttggtctgaa
    30241 agtttttcta aaggagtatc taagctaatt ttattttttt ttgcaaaatc aacaacttgt
    30301 ttaaaaacgc tagcatctct ttcttctccc aaaggcacaa tgcctccttc ttttattgtt
    30361 ttggatttat tgggtaaaac agcatctata ttaatattat attcaactcc taagccttta
    30421 caggttgggc aagcaccata aggcgagttg aatgaaaatg tatttggtga aggttcttcg
    30481 taagaaatac cagtatccac acacattaac tgtttactaa aaacagcatc tttgttgttg
    30541 gatatttgat tggcagatgc aacaaatatt aaatctttac caatttttaa actttgttgt
    30601 atgctttggc ttagccttaa tttcatattc attgtaacct caagtctatc cactacaatt
    30661 tcaatattgt gtactttata tctatccact tgtagtttag gtgttaagtc ttttacttct
    30721 ccatcaattc ttacttttaa aaatcctttt tttctggttt cttcaaacag ttctctatag
    30781 tgtccttttc tgccacgaac caatggtgct aaaattaaaa tcttctcttc tttaaacttt
    30841 tcaaaaatgg tttgtataat ttcctcttcg cttagtttaa ccattttttt ccctgtatta
    30901 tagctataag cttctccaat acgggcaaat agtaaacgca aaaaatcata caattcagtt
    30961 actgtgccta ctgtacttct tgggttttta tttgttgttt tttgttctat agaaataaca
    31021 ggagaaagac cagaaatttt atctacatcg ggccgttcca tattgcctaa aaattgtctt
    31081 gcataagcac caaagctttc catatacctt ctttgcccct cattataaat agtatcaaat
    31141 gctaaagaac ttttaccact cccacttaca cctgtaaaaa ccacaagttt attttttgga
    31201 atagcaatat ctatgttttt taaattattt tctcttgcac cttgtactat aatcttacta
    31261 ttatcaaagt tatcat
//
LOCUS       DAOQPK010000004        27219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_807_length_27219_cov_4.809895, whole genome shotgun sequence.
ACCESSION   DAOQPK010000004 DAOQPK010000000
VERSION     DAOQPK010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 27219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27219
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_807_length_27219_cov_4.809895"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2083
                     /locus_tag="PLU36_00425"
     CDS             <1..2083
                     /locus_tag="PLU36_00425"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HMZ45244.1"
                     /translation="IKKGPLLGQKDQLRTTVLININLSKRIKIDSVKYEFIDSIHHSI
                     KDSVLQNLAMQQYKKSFLKTGEPYTKQKISDELDRLVQWFRTKGYYKFTREHVYALVD
                     TTNENLFKLTLDPIELAKLIVDAEKKRRENPSWKITIKQRVNDDSTRLNKFYVRNSYY
                     YPETKISEIPDSLINSSSFRISKNKKGDLFLKEKEGLFKIKPLREHTYLRKDSLYNEY
                     SFFKTLNTLSSIPAWQQIDARIIPQGKDSLDIHYFLVPQKKYQISYNIEGSRNSGDFT
                     AGNLLGVSLSTSLNNRNVGKLAIQSNTGLRGGVELNLFQQNSQGTKSDLIQTVQFSLS
                     QTYSFPRLVMPRSFLKLPLFKRTENKRTVLTGAIAYTERFQIFRLRSANFSYAWEWQK
                     LLKNNYTALISWKPLNIELYSIDTLPGLKTLFDANPFLRNSFNTGNVIGFPFPGGGLN
                     YILTKPGKRLNSNQLIRFGIEESGLLTLPFKSLQNKVYQYIKSEVEYKYAYKKIKGET
                     VYRAFIGFGIPLRGQSLPFFKQYFAGGPNSMRAWGLRQLGLGSSILSDTLAVNTFRDR
                     FGDMQLEFNAEYRFTLANLGSFKLSSALFADIGNIWNLKKDPTNPNAEFSLSRLYNDL
                     AIGIGTGVRLDFSYFLIRVDFAYKVKDPGRITNNGWMSFKDFVWTEKRNNKNNTEIKN
                     FAIQLGIGLPF"
     gene            2112..3428
                     /locus_tag="PLU36_00430"
     CDS             2112..3428
                     /locus_tag="PLU36_00430"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HMZ45245.1"
                     /translation="MKHILLIGAGKSATVLIHFLIELVQNKNWHLTVADGNEQLLNDK
                     VKTSKNVSILVLKIDNELERKQAIASADIVISMMPPHLHYLIAIDCMEFNKHLLTASY
                     VDEKIQALAKTIKEKNLLFLCEMGLDPGIDHMSAMKLIHEIKEKGGNIVSFKSHCGGL
                     IAPESDDNPWHYKISWNPKNIILAGKAGATYLENGKVVKLPYEALFKNNISVTTSNNK
                     YAYYPNRDSLNYISIYQLQQIKTFVRTTLRHPQFCTGWNMIINLQLTNEVKQYQTNGL
                     SIAKFFETHFKEFNCHEFLKEIKKNDSLHQQLLFIGLNDKDTIINKGLCNAAEVLQFI
                     LEKKLVLNNADKDLIVMLHEFEYLLHNKKYYVTSLLELAGKDNQQTAMAKTVGLPLGI
                     AAKLILENKITTTGLHIPILPEIYLPVLEELQNYGISFNEDSSEIS"
     gene            complement(3497..6037)
                     /gene="topA"
                     /locus_tag="PLU36_00435"
     CDS             complement(3497..6037)
                     /gene="topA"
                     /locus_tag="PLU36_00435"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HMZ45246.1"
                     /translation="MAKNLLIVESPAKAKTIEKILGKDFEVKSCFGHIRDLEKDDMGI
                     DINNHYQPRYIVPDEKLKVVNELKKLSKNKEVWLASDEDREGESISWHLAEVLNLNPL
                     ETKRIVFHEITAPAIKKAVANPRFINMDLVNAQQARRVLDRLVGFELSPVLWRKIGMQ
                     RSLSAGRVQSVAVRLIVEREREINNFNTESSYKVEASLTANDINNKAVNFKAESTKKI
                     NNVINATAFLETCKNANFTVKDVQVKPTKRTPSAPFTTSTLQQEASRKLGYGVSKTML
                     LAQKLYESGKITYMRTDSINLSDTALQDIQKQVIESYGNQYYQPRKFKNKNESAQEAH
                     EAIRPTYMSNDTIADDETRKLYELIWKRTIACQMSDAAFEKTTAIINISTNNEELVAT
                     GEVMKFDGFLKVYFEGKDDDEEDNGEGFLPSLKNGQQLVFNEMTATEKFTRAAPRYTE
                     ASLVKKLEELGIGRPSTYAPTISTIIKRNYVEKRDKDAIKRKVDIVTLTPDFKITNTT
                     ILENTGAEKAKLFPTDLGMLVTDFLKQHFKNVMDYDFTAQIEKEFDEIAEGNKKWSSM
                     IDGFYKPFHETIEHTLENAERAKGERELGIEEATGRKIVARMGRYGAMVQIGEATDEE
                     KPRFAKLNPTQSIETITLEEALALFALPRIIGEYNGQELSVNIGRFGPYIKMGDQFIS
                     IPKNEDLFEIDVERAKLFIEEKQQADAPISTYNEMPVTKGKGRFGPFIKWNDMYINVP
                     RKYNFDNLSKQDIEELIEAKINKEANRYIQQWPSEKISIENGRWGAFIRFNKKMLKLG
                     KKADGTKYDTAELETIDIETIKKMIAEQVPNAFAVKEKKLKTKAAKKK"
     gene            6272..7174
                     /locus_tag="PLU36_00440"
     CDS             6272..7174
                     /locus_tag="PLU36_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HMZ45247.1"
                     /translation="MQPTLVILAAGMASRYGSMKQIQGFGPSGETIMEYSIYDAIQAG
                     FGKVVFIIREEFVDMFKENFGKKLEGKIKVDYVYQQLDSYTFGRTIPEERKKPWGTAH
                     AILCCKEKVNEPFAVINADDYYGKNAFVKAHQFLTTVVAENNYAIIGYELKKTLSENG
                     SVSRGVCDVDEEGNLIEINERTKIYKNEKGVITYEDETGLHEVSEAASVSMNFFGFHP
                     NFILTCEKYFSEFLDNEINNPKSEFFIPLVVDKLVKSNTAKVSVIPTTAQWFGVTYKE
                     DAPGVQASINNLIQQGQYPNNLWA"
     gene            7226..7621
                     /locus_tag="PLU36_00445"
     CDS             7226..7621
                     /locus_tag="PLU36_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45248.1"
                     /translation="MYIIYSRVPDYFDGETAPATIHWLKDSTTNTTIPKAVFIHGKTK
                     YEVDARYVLRSLPENKKLTVIYLVKKPQNAVVYSWWGYWITWGELLTSILLLVALFQV
                     AVAITKNPTPEAVMEQLEYFNAAKKRKYD"
     gene            7664..8158
                     /locus_tag="PLU36_00450"
     CDS             7664..8158
                     /locus_tag="PLU36_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HMZ45249.1"
                     /translation="MKQFHKLLIFTLTFIPLFIACGSTKESTNSELDEVSIAKAERII
                     LELVNKHRKKNDLPKLKAIDELTEIAEKHSKNMGDGKVKVGHGGFDNRYNKIKKEIKN
                     VGSAAENVAFGNTSLKKIVDGWIASPHHKGNIEGPFNYTGVGIYRNKQGVLFYTQIFI
                     NKVK"
     gene            8162..8665
                     /locus_tag="PLU36_00455"
     CDS             8162..8665
                     /locus_tag="PLU36_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45250.1"
                     /translation="MKKLFLLLTVFIFFQANAQKIQEKLFYHSAKGLSFIPPLSKPGL
                     IYEGKLYIGKQRLTGLFYKLNDETLNSYFNKYKANKTAADILTITGGVALPLANIFIA
                     ANEGKVNWWLIAASALLNGTGGYLNMQAQKNLLLSSIYFDKKMGYTTNNFKHKQQVVQ
                     IAIPIGK"
     gene            8698..9453
                     /locus_tag="PLU36_00460"
     CDS             8698..9453
                     /locus_tag="PLU36_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ45251.1"
                     /translation="MQNIVISVKDLNKKYGNFHAVKGISFEVFEGEIFGLLGPNGAGK
                     SSTLEIIETLRPKTSGTVKVVGFDLDTQPNFIKQQIGVQLQTSGFYPGLNLVELIDLF
                     AGLYNEKVNAKELLEQVNLQDKAQAKYKELSGGQKQRFSIATTLINKPKVIFLDEPTT
                     GLDPQARRNLWQLIKKIQAEGTTIIITTHYMDEAEILCNRVAIIDDGKIIAIDTPDKL
                     IDNLVATGFERPKEVKKANLEDVFIHLTGHSIR"
     gene            complement(9459..10022)
                     /locus_tag="PLU36_00465"
     CDS             complement(9459..10022)
                     /locus_tag="PLU36_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HMZ45252.1"
                     /translation="MNKIIVAITGASGSIYAKLLLDSLMQMKNQFAEIGVVMSDNAKT
                     VWQTELKNEDYKNYDVTFYSKNNFNAPFASGSAKFNIMIVAPCSMGTLGRIASGISDD
                     LITRAADVILKERRKLILMVRETPFNLIHLKNMETITLAGGIICPAIPSFYSLPTSIE
                     CLAKTVTDRVLELAGLEINTFRWGKQL"
     gene            10434..10721
                     /locus_tag="PLU36_00470"
     CDS             10434..10721
                     /locus_tag="PLU36_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HMZ45253.1"
                     /translation="MEEKTKINIEEGVVHNLPDDIKEVLITNIDLLEKWNKLTPLARN
                     EWICWVTIVKQEHTRKEHILRLQEDILSGKKRPCCWPGCPHRRESAKKWFK"
     gene            complement(10928..12583)
                     /gene="glgP"
                     /locus_tag="PLU36_00475"
     CDS             complement(10928..12583)
                     /gene="glgP"
                     /locus_tag="PLU36_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HMZ45254.1"
                     /translation="MNFKNFQVPYHISDQYAKKAAYFSMEFAIHQPLKIYSGGLGFLA
                     GSHLRSAYELKQNMIGIGILWKYGYYDQTRNQDQTLQPVWLEKNYHFLEDTGIKFQIK
                     IHDAPVWVKIWYLNPETFGSAPLFLMSTDLPENDYVSQTITHRLYDANVSTKVAQFIL
                     LGVGGAKLLEEIGFNTDVYHLNEAHGISAAFYLLANKYKTVNALKEHLVFTTHTPEEA
                     GNEKHDIYLCHKMSYFCGLTVDEVKKLTGQEEDQFNHSLVALRFARKANAVSQLHGVV
                     SNKMWSKYTNICPITAITNAQNFSYWADEPLYRHLDANNDWGIDDRKSYLKKRAFENV
                     ADQTGKLFNPEVLTIVWARRFAGYKRADLLTHDMERFEKLVNNKNFPVQIIWAGKPYP
                     ADYPAISQFNELVHLSKKYKNVSVMIGYELLLSRRLKQGADVWLNNPRVPREASGTSG
                     MTAAMNGAVNFSTDDGWIPEFAKPGTNSFVIPKADYENMTQHDQDIYDLYRMYDILES
                     EIIPMYYNDKDAWRQMVKNGMNDVRVQFNSNRMAHEYYEIMYK"
     gene            complement(12772..13929)
                     /locus_tag="PLU36_00480"
     CDS             complement(12772..13929)
                     /locus_tag="PLU36_00480"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HMZ45255.1"
                     /translation="MAYYYKSGNIPPKRHIQFRKVDGSLYSEQVFSTEGFSDDYSILY
                     HCHPPTQIIKTDTPIDVAPIIAEEKMLKHRSFEGMNIKPVNDYLQSRIPVLVNNDCHM
                     VLAAPQKSMQDYFFKNADADEVIFIHEGSGKLLTQYGELSFSYGDYLVLPRGTIYQIT
                     FNDSNNRLLIVESFSPIRFPKRYMSKHGQLLEHSPFCERDIRAPQNLITKDEQGDFII
                     KTKKKGILYGLHYANHPFDVVGWDGCCYPYAFSIHDFEPITGRVHQPPPVHQTFEGNN
                     FVICSFVPRLYDYHPLAIPAPYHHSNIDSDEVLYYVDGDFMSRKNVTRGMITLHPAGI
                     PHGPHPGAIEKSIGKLETKELAVMLDTFHPLMLTKQALEIENENYTMSWAE"
     gene            complement(14125..15006)
                     /locus_tag="PLU36_00485"
     CDS             complement(14125..15006)
                     /locus_tag="PLU36_00485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HMZ45256.1"
                     /translation="MKGYINNIIKGNTLSVLKTIDSNLIDIGITSPPYNKQEKHKGWL
                     VKNVQYDSYKDIKTEEEYQEEQIEILNEIFRITKEGGSFFYNHKTRWERGQMFHPIQW
                     LAKTNWTIRQEIIWDRMIAANIRGWRYWQVDERIYWLYKPINKNKIGIELESKHALLT
                     SIWRFPPENGKKNPHPAPFPLTLPSRIITSMLNDTDGIVLDPYCGSGTTLVAASLLNK
                     KYIGIDISEDYIQYAKDRLQNASSEMKIVLEELQKHIVQKTFKQRKQQGAFTGKHKNN
                     SNTEEVLQATVKQLHLL"
     gene            complement(15006..15659)
                     /locus_tag="PLU36_00490"
     CDS             complement(15006..15659)
                     /locus_tag="PLU36_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45257.1"
                     /translation="MNQDLNQIITYYATKPHKVLNELLLNKSKDNLIAILTDLLTAYI
                     NDKNSSSLREFITVSIAGYQHNSKKLGYNGYKQNSAIGSKPISCEAKPKNIQTEGYEL
                     KKSKPKLNGEGGFNDYTIERLNKDVKEKLNLLSSGFIDGELQYIIEFPFKAVYNKLRE
                     QLPERRIVGTYTRMASFNFSHYSSYPVIKIIYLNKNAIERNQQYFNKKFYQFLIDSK"
     gene            15816..16472
                     /locus_tag="PLU36_00495"
     CDS             15816..16472
                     /locus_tag="PLU36_00495"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HMZ45258.1"
                     /translation="MIKREDTYRHKGLRKQLIDILKEKGITQENVLNAINNIPRHFFL
                     DPAFDNIAYEDRAFPIGEGQTISQPYTVAYQTQLLNVKKFDKVLEIGTGSVYQASVLA
                     ELGASVFTIERQKNLYVKNNKFIFKNKYLNIKFFYGDGFEGLPQFAPFDKIIITAAAP
                     FIPTKLIEQLKIGGQMVIPVDEGEVQRMLRLTKQKDGTLLEEEFEKFSFVPMLKGKNS
                     "
     gene            16508..17857
                     /locus_tag="PLU36_00500"
     CDS             16508..17857
                     /locus_tag="PLU36_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HMZ45259.1"
                     /translation="MLPVNFIQTIQHINGFDREGFEAVHTNSNPITSIRLNDEKVCNN
                     LSYLYQQQVPWCNHAYYLKERPSFTLEPSFHAGAFYVQEASSMFLWHIIKQLYANNTE
                     KKVLDVCAAPGGKSTLLASYFKNGLIVSNEIIKHRAAILVENLSKWGTGNFIVSNNDP
                     KQFANLTSFFDAVVIDAPCSGSGMFRKDKTAVDEWSLQNVKLCSQRQQRIIANVMTAL
                     KENGYLIYSTCSYSEEENEQILDFIADNFSVESVEININPSWGIVSTKSNKHKAIGYR
                     FFPDKIKGEGFFIAVFKKNEFSKNNKFSLQLLSKLTSVENELIKNFITSTIDKKFFKH
                     NNEIKCINTAFYADVEKLANLLYLKKVGTIIGEVKGKDFVPHHELAISNISLSNIATL
                     ELNKEDALKFLAKKELDINTNKGLTLITFNGLGLGWIKVLHNRINNYYPNEWRILKI"
     gene            17880..18821
                     /locus_tag="PLU36_00505"
     CDS             17880..18821
                     /locus_tag="PLU36_00505"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HMZ45260.1"
                     /translation="MKKIFCIVLTFFILNQSFAQNKILVQGSYPNLYLQHKVAHGETM
                     YSISKLYNFSSAQLATQNGMTENDILSTDKILKINLGKNNFTQDGQSGENETLIPLYH
                     IVKPGETLFRICQNFGKIRIDFIRGWNDLVNDIIKPEQKIIIGHLRVDLKKASIVTER
                     IINTDAQETATAIDQTEPPKKDETETTTSTTPKQEEEEPVVEPVTNGNDDEGFFVTSY
                     SVNSDNSVSTTGEAATFKSTSGWTNRKYYVLCNDIPTGTIVRITANNNRSICAKVLGA
                     LPEMKENKNLIIRISNAGAAVLKITEKRFNVKITYNK"
     gene            complement(18825..19712)
                     /locus_tag="PLU36_00510"
     CDS             complement(18825..19712)
                     /locus_tag="PLU36_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45261.1"
                     /translation="MSSSEIDIFKWDNCVQENSNGLIYAYSFYLNTMAENWHGLIIND
                     YEAIFPIPVKNKLGIQYAYMPAFTQQLGLIGNVKINNTKLINAIHSFLKYGSINLNFS
                     NKNFTEKIVEKNNFIIDLKNNYSVIQEKYKKTIAYSLSKAAKHHFIYTKDDDIKKAVA
                     LYKLYNQNNMPHVIDIDYTNLKKLLLQLQKNNNVLVRKVENEKQELMSIALLMKDIKR
                     YYNILNTTTEAGRKTEANYFLYNNILQELCNQNMLFDFEGSDLPGVKKFYEKFGAINQ
                     PYFNWHFNNLPIPFKWLKK"
     gene            complement(19736..22576)
                     /locus_tag="PLU36_00515"
     CDS             complement(19736..22576)
                     /locus_tag="PLU36_00515"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HMZ45262.1"
                     /translation="MKNRIVLLLLMLFCVINSFSQNNYWQQKVDYNLKVSLNDTANTL
                     DGFATITYYNNSPDTLQFIWFHLWANAYKNDKTAYTKQALENGSTTFYFSNDEDRGYI
                     NQLNFKVNNVTALTEVHQQYTDIIKLILPSALPPQQSITITTPFFVKIPALFSRMGVS
                     KQNYFITQWYPKPAVYDRKGWHEMPYLDQGEFYSEFGDFDVQIILPDSYIVAATGELQ
                     QTDLLHTLKKLGKEKPEKQANYLNYLKEEKQKKISPSKTSKVTATKHSPTLTAYNFKQ
                     TNCHDFAWFASKNFILQYDTVKLTTKTVDVFTFYLPQQLKAWNKSIAYAKNGIKKYSN
                     WVGEYAYNTVSVVAGFENEFSGGMEYPTITVITTNTEGKRLDETIVHEIGHNWFYGVL
                     ASNERKHAWMDEGMNTFYEKKYMKEQYASNNKSLLSNYSIDVLEKVANRTITKIKKSQ
                     PIDTISTAYSNLNYFLIVYSKASSWMKQLETTLGEDSFKEVMQQYYNDWKFKHPYPED
                     FKKIVINKNKEAEFLFSYLYNKKNLDTVSYNKKIKPIFIASLKETEKYNYIGISPLAA
                     YNYYDKVSIGAFIHNYQLPLNRFQFFVAPLYAFGSKEFNFNARASFNTYKEKYWLEFA
                     TSASKFTIDNFKPTNAATINQQVVKIVPSVKYTLYRNDKRSTLRWVFQARSFLLQENG
                     LLFKTLNGVDIVEKTTENSYINQFKITTLDNRILYPYNFNLTIDQGKSFVRAGFTANY
                     HFNYKNRKGGIDARFFAGKFFYTSDKTFIKQYETDRYHLNMSGAKGYEDYTYSNYFIG
                     RNEFEGWQSQQIMQRDGFFKVSTDLLSNKIGKTDDWLMALNLSGNIPDAINILNVLPI
                     KIPLKFFVDIGTYAESWKENPATGRFLYDAGLQLPLIKGLVNIYIPILYSKVYADYFK
                     STLGSKRFSKTISFSIDLQQLQLNKLSRYIPL"
     gene            22701..24005
                     /locus_tag="PLU36_00520"
     CDS             22701..24005
                     /locus_tag="PLU36_00520"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HMZ45263.1"
                     /translation="MYPNLYYFFKEVFGIEIEGFKIINSFGFFVAIAFIGAAWALTQE
                     LKRKQKQGLFVYTEEEIIVGKPASASELMIHFFLGFIFGFKILGGFFLPNAFSDPQLY
                     IFSGQGNWWMGLLVGGVFAWIKYNEKNKLKLPTPEKRTIRIWPHDRVGDLVIYAAIFG
                     FAGAKLFDILEAPSAFFSMFKAVKEGKQDVGSLLFSGLTFYGGLIVATIAIIVYAKKH
                     KIGIIYLADAFAPAMMLAYALGRIGCQVAGDGDWGIVNSAFISNNIGESILATPEQFT
                     NALHTHSAYLSSKFGTIEQMHHMSIQPFLGLPNWMFGYTYPHNVVGDGVALANCSGVH
                     CNYLPLPVFPTPFYELTACLILFLILWAVRSKLKIPGQLAGLYLIFNGTERFFIEKIR
                     VNATYNLAGMKITQAEIISLILVIAGVVLLTMSKKWFGKLAK"
     gene            24118..25956
                     /gene="glmS"
                     /locus_tag="PLU36_00525"
     CDS             24118..25956
                     /gene="glmS"
                     /locus_tag="PLU36_00525"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HMZ45264.1"
                     /translation="MCGIVGYTGKRQAYPVVLKGLKRLEYRGYDSAGVALKNERGLHI
                     YKKKGKVAEMEESVVGKDVDSTIGIGHTRWATHGEPSDRNAHPHQSNDGKLAMIHNGI
                     IENYASLKQDLINKGYTFSSDTDTEVLLNFIEDIQKNNNCPLEEALRIALKRIVGAYC
                     ILLISEDDPETIIAARKGSPLVIGIGKGEHFLASDASPIIEYTKEVVYVNDYEVAIVK
                     PNELILKNLGNEKQTPFITKLDMELAAIEKGGYNHFMLKEIHEQPDTIYDCLRGRLLP
                     DTGQIMMSGIENNIDALKNAQRIVMVACGTSWHAGLIAEYMIEELCRIPVEVEYASEF
                     RYRNPIIHKGDVIVAISQSGETADTLVALERAKEQGAFIFGVVNVVGSSIARISHAGA
                     YTHAGPEIGVASTKAFTGQLAVLAMMALKIANAKQTITQDRYIQLMKDLHNIPEKVKE
                     ILADTSTIERIAEKYKNASDFLFLGRGYNFPVALEGALKLKEISYIHAEGYPAAEMKH
                     GPIALVDENLPVVFVATKDSYYEKIVSNVQEIKARKGQIIAVATNDDTVIPGMANDVM
                     FVPDTDEVIAPLLSVIPLQLLSYYVGVAKGLDVDKPRNLAKSVTVE"
     gene            26018..>27219
                     /locus_tag="PLU36_00530"
     CDS             26018..>27219
                     /locus_tag="PLU36_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HMZ45265.1"
                     /translation="MPQNNIKKSHLSSLGYGFIKNEHIPKGKDEYYLRDLQNKSGINY
                     RQLTAYEIEVLVRNRNTSDNWNNILVSNAFNPELVNDCKFYGLVRIGKLEPFCLEYSD
                     LKVPVGLYNSTIISCDFGDNVCIDNVRYLSHYIIGNEVIIVNVNELTTTNFAKFGNGI
                     IKQNETENRRIWLEICNENAGRSILPFNGMLTADAFLWSKYRDDVELLNKFKEFTEAR
                     FDNRTGYYGKVGHRTVIKNTAIIKDTWIGNDAYIKGSNKLKNLTINSGEEGKTQIGEG
                     CELVNGIIDYGCRVFYGVKAVRFIMASHSQLKYGARLINSYLGNNATISCCEVLNSLI
                     YPAHEQHHNNSFLCAALIMGQSNIAAGATIGSNHNSRAADGEIVAGRGFWPGLCVSLK
                     HNSQFASYT"
BASE COUNT         9556 a         4021 c         4117 g         9525 t
ORIGIN      
        1 tataaagaaa gggcctttgc ttggacaaaa agaccaacta cgaacaactg tactaataaa
       61 tattaattta agtaaaagaa taaaaattga ctcggtaaaa tatgaattta tagattccat
      121 tcatcatagc attaaagatt ctgtattgca aaacctggca atgcaacaat ataaaaaatc
      181 ttttttaaaa actggtgaac cttatactaa acaaaaaata tctgatgagt tagacaggct
      241 ggtacaatgg tttagaacca aaggttacta taagtttacc agagagcatg tatatgcttt
      301 agttgatacg actaatgaaa atttatttaa acttacttta gaccccattg aattagcaaa
      361 actaattgtt gatgcagaaa agaaacgaag agaaaatcct tcttggaaaa ttactatcaa
      421 gcaaagagta aacgatgatt caacacgatt aaataaattt tatgtaagaa attcttacta
      481 ctatccagaa acaaaaatta gcgaaatacc agactcttta ataaatagtt caagttttag
      541 aatttcaaaa aacaaaaaag gtgatttgtt tttgaaagaa aaagagggat tgtttaaaat
      601 taagccatta agagaacata cttatttaag aaaagacagt ttgtataatg aatatagttt
      661 ttttaaaaca cttaatacac tttcttctat tcctgcatgg cagcaaatag atgccagaat
      721 aataccacaa ggaaaagatt ctttagatat tcattatttt ttagttcccc aaaaaaaata
      781 tcaaataagc tataatattg aaggtagtag aaactcagga gattttacag caggtaattt
      841 actaggcgtt tctttaagta cttctcttaa taacagaaac gttggaaagc ttgcaataca
      901 aagcaataca ggtttgcggg gtggtgttga attaaattta tttcaacaaa attcacaagg
      961 cactaaatca gatttaattc aaacagtaca atttagtttg tctcaaacat atagctttcc
     1021 tagattagta atgccaagaa gctttttaaa acttccatta tttaaaagaa cggaaaataa
     1081 acgaaccgtt ttaacaggtg caatagccta tacagagagg tttcaaattt tcagacttag
     1141 atctgccaat tttagctatg catgggaatg gcaaaaactt cttaaaaata attatactgc
     1201 cttaatatct tggaagccct taaacattga gttatatagt attgacactt tacccggttt
     1261 aaaaacttta tttgatgcaa atccattctt aagaaattca tttaatactg gtaatgtaat
     1321 tggtttccct tttcctggag gtggtttaaa ttatatatta actaagccag gaaaaagatt
     1381 aaacagtaat caattaatta gatttggtat tgaagaaagt gggttattaa ccctaccttt
     1441 taaatcatta caaaataaag tgtatcagta tattaaatca gaggtagaat ataaatatgc
     1501 ttacaaaaaa ataaaaggag aaactgttta ccgtgcattt ataggttttg gtattccatt
     1561 aagagggcaa tcattaccct tctttaaaca atactttgca ggaggcccaa atagcatgag
     1621 ggcatggggt ttaagacaat taggtttagg ctcatctata ctaagcgaca cccttgctgt
     1681 aaatacattt agagatagat ttggcgacat gcaacttgaa tttaatgcag agtatcgttt
     1741 tactttagcc aaccttggaa gttttaaatt atccagtgca ttatttgcag atattggtaa
     1801 tatttggaat ttaaagaaag atcctaccaa ccccaatgct gagttttcat taagcaggtt
     1861 atacaatgat ttagcaatag gtattggaac tggtgtaaga ttagattttt cttatttttt
     1921 gattagggtt gattttgctt ataaagtaaa agacccagga agaattacca ataatggttg
     1981 gatgagtttt aaagattttg tttggacaga aaaaagaaat aataaaaata atactgaaat
     2041 aaaaaacttt gccatacagt taggaatagg cttaccattt taattatttt taattaccat
     2101 tgcaccttaa tatgaaacat attttattaa ttggtgcagg aaaatctgct actgttttaa
     2161 ttcatttctt aattgaatta gttcaaaata aaaattggca tttaacagta gccgatggca
     2221 acgaacaatt gttaaatgat aaagttaaaa caagtaaaaa tgtatctata cttgttttaa
     2281 aaatagataa tgaactggaa agaaaacaag caattgcatc tgcagacatt gttatttcta
     2341 tgatgccacc acatttgcat tacttgattg caatagattg tatggagttc aataaacatc
     2401 tactcacagc atcttatgta gatgaaaaaa ttcaggcttt agcaaaaaca attaaagaaa
     2461 agaatttact ttttttatgt gaaatgggct tagaccctgg catagaccac atgagtgcca
     2521 tgaaattaat tcatgaaata aaagaaaaag gaggaaatat tgtttcattt aaatcgcatt
     2581 gtggaggttt aattgcacca gaaagcgatg acaacccttg gcattataaa ataagttgga
     2641 acccgaaaaa tattattctt gctggtaaag ctggtgcaac ttatcttgaa aatggcaaag
     2701 tggtgaaatt gccatatgaa gcacttttta aaaacaatat ttctgtaaca acaagtaata
     2761 ataaatatgc ttattatcca aacagagatt ctttaaatta tatttcaatt tatcaattac
     2821 agcaaattaa aacttttgta agaaccactt taaggcatcc acaattttgc acaggatgga
     2881 atatgattat taatttacaa ttaactaatg aagtaaaaca ataccaaaca aatggcttaa
     2941 gtattgcaaa gttttttgaa actcatttta aagaatttaa ttgccatgaa tttctaaagg
     3001 aaattaaaaa aaatgattca ttgcaccaac agcttttatt tattggttta aatgataagg
     3061 ataccatcat taataaaggt ttatgtaatg ctgctgaagt actacaattt attctcgaaa
     3121 aaaaattagt tttaaacaac gcagataaag atttaattgt aatgcttcat gaatttgaat
     3181 atttgctaca caataaaaaa tattatgtaa caagtttatt agaacttgca ggaaaagaca
     3241 atcagcaaac tgcaatggcc aaaacagttg gtctgccatt aggaatagca gcaaaattaa
     3301 ttttagagaa taaaataaca acaacaggct tgcatattcc aatattacca gaaatatatt
     3361 tacctgtttt ggaggaatta cagaattatg gaatttcttt taatgaagat agttctgaaa
     3421 tttcataagt ttaataatta tttaaaacct attcatttaa gccaaaagaa ttctattaat
     3481 aaaaatgcaa taagggctat ttctttttag cagctttggt ttttaatttt ttttctttaa
     3541 cagcaaaagc attaggtact tgttctgcaa tcattttctt aattgtttca atatctatag
     3601 tttctaattc tgcagtatcg tattttgtgc catctgcttt tttaccaagt ttcaacattt
     3661 ttttattaaa cctaataaaa gcaccccatc taccattttc aatgctaatt ttttccgaag
     3721 gccattgttg aatgtagcgg ttggcttctt tattaatttt tgcttcaatc aactcttcaa
     3781 tatcctgctt actaaggttg tcaaaattat attttcttgg aacgttaata tacatatcat
     3841 tccatttaat aaatggtcca aatcttcctt tgccttttgt aacaggcatt tcattatagg
     3901 ttgaaattgg agcatcggcc tgttgttttt cttcaataaa taatttggca cgttctacat
     3961 caatttcaaa taaatcttca tttttaggaa tagaaataaa ctgatcaccc attttaatgt
     4021 aagggccaaa tctaccaata tttacagata attcctgacc attatattca ccaattattc
     4081 ttggtagggc aaataaagcc aatgcttcct ctaaagttat agtttctatg ctttgtgtgg
     4141 ggtttaattt tgcaaagcgt ggtttttctt catcggttgc ctctcctatt tgaaccattg
     4201 caccataacg tcccatacga gcaactattt ttctccctgt tgcttcttca attcctaatt
     4261 ctctttcgcc ttttgctctt tctgcatttt ctaaagtgtg ttcaatggtt tcatgaaagg
     4321 gtttataaaa tccatctatc atactactcc acttcttatt accttctgca atttcatcaa
     4381 actctttttc tatttgtgca gtaaaatcat aatccattac gtttttgaaa tgctgcttta
     4441 aaaaatcggt aacaagcatt cctaaatctg taggaaaaag tttagctttt tcagcaccag
     4501 tattttctaa aattgttgtg ttagtaattt taaaatctgg tgtaagtgtt acaatatcta
     4561 cttttctttt aatcgcatct ttatctcttt tttcaacata atttctttta attatggtgc
     4621 taatggttgg tgcataagta cttggacggc caatacctaa ctcttctaat ttttttacta
     4681 aacttgcttc cgtatatctt ggtgcagctc ttgtaaattt ttctgttgca gtcatttcat
     4741 taaaaacaag ttgctgtcca ttttttaatg aaggtaaaaa tccttctcca ttatcttctt
     4801 catcatcgtc tttaccctca aaataaacct ttaaaaaccc atcaaatttc attacttcac
     4861 cagttgcaac tagctcttca ttgttagtgc ttatattaat aatagcagta gttttttcaa
     4921 atgctgcatc actcatttgg caagcaattg ttcttttcca aattagttcg tacagctttc
     4981 ttgtttcatc atcagcaatg gtatcattac tcatgtaagt aggccttata gcttcatgtg
     5041 cctcttgtgc agattcattt ttatttttaa actttcttgg ctgataatat tggttgccat
     5101 aagactctat tacttgcttt tgtatatctt gcaatgcggt atcactaagg ttaatactat
     5161 ctgtacgcat gtaagtaatt ttaccacttt catatagctt ttgtgccaat aacattgttt
     5221 tacttacccc atatcctaat ttccttgaag cttcttgttg taatgtagat gttgtaaaag
     5281 gtgctgatgg tgtgcgtttg gtaggtttta cctgcacatc ttttactgta aaatttgcat
     5341 ttttacaggt ttccaaaaat gcagttgcat taattacatt attaattttt ttagtgctct
     5401 ccgctttaaa attaactgcc ttattattaa tatcattggc agttaaacta gcttcaacct
     5461 tataactact ttctgtatta aagttattta tctctctctc tctttcaaca attaatctta
     5521 cagccacact ttgtacacgg cctgcactta aacttctttg cataccaatt ttcctccaca
     5581 aaacagggct tagttcaaag cctaccaatc tgtctaatac tctacgagct tgctgggcat
     5641 tcactaaatc catattaata aaacgtggat tggcaacagc ttttttaata gctggtgcag
     5701 taatttcgtg aaaaacaatt cgtttagttt ctaatggatt tagatttaaa acttctgcta
     5761 aatgccaact aatgctctct ccttctctat cctcatcgga tgctaaccaa acttctttat
     5821 tttttgaaag cttttttaac tcgtttacaa ctttaagttt ttcatcaggt acaatatatc
     5881 ttggttggta atgattatta atatcaatac ccatatcatc tttttctaaa tctctaatat
     5941 ggccaaaaca acttttcact tcaaaatctt tacccaatat tttttcaatt gtttttgctt
     6001 ttgccggaga ctctactatt aataaatttt tagccatatc ttatacttcc tctttcaaaa
     6061 tttaaatact caaataaata atcaagaaca attttagctt aataaaattt tacttaaact
     6121 actgcaatat tagccatata gctataaaaa gcaaaaacgg aagttgattt gataataatt
     6181 aataaatatt ttttatgaag aaatctaatt atagaaggta taaattattc tatattaaac
     6241 aattatcttc gcagcatttt aaattaaaat tatgcaacct acattagtaa ttttagctgc
     6301 aggaatggca agccgttatg gcagtatgaa acaaatacaa ggttttggac caagtggtga
     6361 aacaattatg gaatacagta tttacgacgc catacaagca ggttttggca aagttgtatt
     6421 tattataaga gaagaatttg tagatatgtt taaagaaaat tttgggaaaa aattagaagg
     6481 caaaataaaa gtagattacg tttatcaaca attagatagt tatacttttg gaagaactat
     6541 accagaagaa agaaaaaaac catggggcac cgcacatgcc atactttgtt gcaaagaaaa
     6601 agtaaacgaa ccatttgctg taattaatgc agatgactat tatggtaaaa atgcttttgt
     6661 taaagcacac caatttttaa ctacagttgt tgccgaaaac aattatgcaa ttataggcta
     6721 tgaacttaaa aaaacattaa gtgaaaatgg aagtgtgagt agaggtgttt gtgatgtaga
     6781 tgaggaagga aatttaattg aaattaacga gagaactaaa atttacaaaa atgaaaaagg
     6841 agtaattact tatgaagacg aaactggttt acatgaagta agtgaagctg caagtgtaag
     6901 tatgaatttt tttggatttc atcctaattt tattctaacc tgcgaaaaat attttagtga
     6961 atttttagat aatgaaataa ataaccctaa aagcgaattt tttattcctt tagtggtaga
     7021 taaattggta aaaagcaata cagcaaaagt atctgtaata ccaacaacag cacaatggtt
     7081 tggtgttacc tataaagaag atgcaccagg tgtacaagca agtattaata atttaattca
     7141 gcaagggcag taccctaata atctttgggc ttagtggttt attatgtata aaggcattgc
     7201 ttttttatat tttgtatgct ttaccttgta tattatttac agtagggtac cagattattt
     7261 tgatggagaa actgcacctg caactatcca ttggctaaaa gacagtacta caaacacaac
     7321 tatacctaaa gcagttttta ttcatggtaa aacaaaatat gaggtagatg cccgttatgt
     7381 tttaagaagc ctaccagaaa ataaaaaact aactgttatt tatcttgtta aaaagccaca
     7441 aaatgcagtt gtatatagtt ggtggggcta ttggattact tggggcgaac tgctaacttc
     7501 tattctttta ttagttgccc tttttcaggt tgcagttgca ataaccaaaa accctacacc
     7561 agaggcagtt atggaacagc tagaatattt taatgctgct aaaaaaagga agtatgatta
     7621 atgtttaaaa acaattttaa tactaccttc acaaaaaaaa tttatgaaac aatttcataa
     7681 acttttaatt tttacactaa cttttattcc tttatttatt gcttgtggta gcactaaaga
     7741 atctaccaac tctgaattgg atgaagttag tattgctaaa gcagaaagaa taattttaga
     7801 attggtaaat aaacatcgga aaaaaaatga tttaccaaaa ttaaaagcaa ttgatgaact
     7861 aaccgaaata gctgaaaagc atagtaaaaa tatgggagat ggaaaagtaa aagtaggaca
     7921 tggtggtttt gacaatagat ataacaaaat aaaaaaagaa attaaaaatg ttggaagtgc
     7981 tgcagaaaat gttgcttttg gaaataccag tttaaaaaaa attgtagatg gttggattgc
     8041 aagcccacat cataaaggta atatagaagg cccctttaat tatacaggtg ttggaattta
     8101 tagaaataaa caaggtgtgt tattttatac ccaaatattc attaataaag taaaataact
     8161 aatgaagaaa ttatttttat tgcttacagt atttattttt tttcaagcca atgcccaaaa
     8221 aattcaagaa aaactatttt accatagtgc caaaggctta agctttattc caccattatc
     8281 aaagccaggt ttaatttatg aaggtaaact ttatataggt aagcaaaggc ttactgggtt
     8341 gttttataaa ttaaatgacg aaacattaaa tagctacttc aataaatata aggctaataa
     8401 aactgctgca gatattttaa caattactgg tggcgttgct ttgcctttag ccaatatttt
     8461 tattgcagcc aatgagggca aagtaaattg gtggcttatt gcagcaagtg ctttgttaaa
     8521 tggaactggt ggatatttga atatgcaagc tcaaaaaaat ctattacttt catccatata
     8581 tttcgataaa aaaatgggtt atactactaa taattttaaa cacaaacaac aagtagtaca
     8641 aatagcaata cccattggta aataatttta attctaaaaa gaaagcaatt ctattatatg
     8701 caaaatatag ttattagtgt aaaagattta aataaaaaat atggcaattt tcatgcagta
     8761 aaaggaatta gttttgaggt atttgaaggt gagatttttg gtttgttggg gcctaacggt
     8821 gcaggtaaat caagtacttt agaaattatt gaaaccctta ggccaaaaac aagtggcact
     8881 gtaaaggttg ttggttttga tttagacaca cagcctaatt tcattaaaca acaaataggt
     8941 gttcaacttc aaacaagtgg tttttaccct ggtttaaatt tagttgaact gattgattta
     9001 tttgctggat tatacaatga aaaagtgaat gctaaagaat tattagagca ggtgaattta
     9061 caagataagg cccaagcaaa atataaagaa ttaagtggtg ggcaaaagca gcgtttttct
     9121 attgctacaa cacttattaa taaaccaaaa gttatttttt tagatgaacc aacaacaggt
     9181 ttagaccctc aagccagaag aaatttgtgg caattaatta aaaaaattca ggcagaaggt
     9241 actaccatta ttattactac tcattatatg gatgaagctg aaatactgtg taatagagta
     9301 gcaattatag atgatggaaa aataattgct attgatacac cagataaatt aatagacaac
     9361 ttagttgcca caggttttga aagacctaaa gaagtaaaaa aagctaattt agaagacgta
     9421 tttattcatt taacaggcca ctctataaga taaatttttt acaattgttt accccaccta
     9481 aatgtattta tctctaaacc tgccaactca agaaccctat ccgttacagt tttagcaaga
     9541 cattcgatgc tggtaggtaa agaataaaat gatggaattg cagggcagat aataccacct
     9601 gccaaggtga ttgtttccat attttttaag tgaattaaat taaatggtgt ttctctaacc
     9661 ataagaatta gttttcgtct ttctttcaaa ataacatcag cagccctggt tattaaatca
     9721 tcacttatac ccgatgctat tctgcctaaa gtgcccatgc tacatggagc aactatcata
     9781 atattaaact ttgcagagcc agaagcaaaa ggtgcattga aattattttt agagtaaaaa
     9841 gttacatcat agtttttata atcttcattt ttaagttcag tttgccaaac tgttttggca
     9901 ttatcgctca taacaacacc aatttcggca aattgatttt tcatttgcat taaactatct
     9961 aatagtaatt tagcgtaaat actaccactt gcccctgtta ttgctacaat aattttattc
    10021 atgttccaaa aatacattaa acattaaaag taatttttaa aacattaata cttgatgcaa
    10081 cctttaaact gtatgcatcg tctataatat ggtttttcat agaatattgg atttaaacag
    10141 ggcaggattt ctatcctaag ccctcttttt atttatagta atattagtaa ataatctacc
    10201 atcaaataat gtaagatatg aactaatgta aacgattact ctactcttct ctacaaaagt
    10261 catcacaatt tttgcagctt caatcttctt taaaattttt taacctaaca taattagtaa
    10321 agaaaaatta catttatatt agaagccaat tagcttaata gcaagccaac gtaattccag
    10381 cacattaata aataataatt aatttttaat taattagaaa aatgaaataa aaaatggaag
    10441 aaaaaacaaa aataaacata gaagaagggg ttgtacataa tctaccagac gatattaaag
    10501 aagtattaat tactaatatt gaccttttag aaaaatggaa caaactaact ccacttgctc
    10561 gtaatgagtg gatttgttgg gttacaatag taaaacaaga acatactaga aaagaacata
    10621 tattacgatt acaagaagat attttgagtg gtaagaaacg accttgttgt tggccaggtt
    10681 gtccacatcg tagagagagt gcaaagaaat ggtttaaata atctcactta ttatacaaaa
    10741 aagctaaatg aattttataa aatttataca catttaaatt acttgcaact atttaataaa
    10801 taaaagcatc tattatttgt ttataaattt attacattct aaattacttt ttacaaaggt
    10861 tgaagtgtac aaaaaaactc cgaatgaaat atttcggagt tttgcttttt ggtttttcta
    10921 tacactatta tttatacatt atttcgtaat attcgtgagc cattctgttg ctattaaatt
    10981 gaactcttac atcattcatt ccatttttta ccatttgtct ccaagcatct ttatcattgt
    11041 aatacatagg aattatttca ctttctaaaa tatcgtacat tctgtataaa tcataaatat
    11101 cttgatcgtg ttgggtcatg ttttcataat ctgctttagg tataacaaag ctattagtac
    11161 caggtttagc aaattcagga atccatccat catctgtact aaagtttaca gcaccattca
    11221 tggcagctgt cataccactt gtgccacttg cttctcttgg tacacgaggg ttgtttaacc
    11281 atacatctgc cccttgtttc aatcttcggc ttaataataa ttcgtaaccg atcattacag
    11341 aaacgttctt atatttttta cttaaatgaa ccagttcatt aaactgagaa attgctggat
    11401 aatctgccgg ataaggtttt cctgcccaaa ttatttgtac cggaaagttt ttattattta
    11461 ctaatttctc aaaacgttcc atatcatgcg tcagtaaatc ggctctttta taacctgcaa
    11521 aacgtcttgc ccaaacaatg gttaaaacct ccggattaaa taattttccg gtttgatctg
    11581 ctacgttttc aaaagctctt ttctttaaat agctttttct atcatcaatg ccccaatcgt
    11641 tattagcatc taaatgtcta tacaaaggtt catctgccca ataactaaaa ttttgtgcgt
    11701 tggttattgc agtaatagga caaatatttg tgtatttact ccacatttta ttacttacaa
    11761 caccatgtaa ttgtgataca gcattggctt ttcttgcaaa ccttaaagca accagtgaat
    11821 gattaaactg atcttcttct tggcctgtta gctttttcac ttcatctact gttaagccac
    11881 agaaataact cattttatga cataaataaa tatcatgttt ttcattgcct gcctcttctg
    11941 gtgtgtgtgt ggtaaaaact aaatgctctt ttaaagcgtt tactgtttta tatttatttg
    12001 ctagtaaata aaatgcagca gaaataccat gagcttcgtt taagtgatat acatctgtat
    12061 taaaaccaat ttcttctaaa agctttgcac cacctacacc taataaaata aactgtgcta
    12121 ctttggtaga tacattggca tcgtataaac ggtgtgtaat tgtttgcgaa acataatcat
    12181 tttctggcaa atcggtactc attaaaaata atggtgcact accaaaagtt tctgggttta
    12241 agtaccatat tttaacccat actggagcat catgtatttt tatttgaaat ttaattcctg
    12301 tatcttctaa aaaatggtaa tttttttcta accatacggg ttgtaaagtt tggtcttgat
    12361 ttcttgtttg gtcgtaataa ccatatttcc ataaaatacc tataccaatc atgttttgct
    12421 ttaattcata agcactacgt aaatgagagc cagctaaaaa gcctaaacca ccactgtaaa
    12481 tttttaaagg ttggtgtatt gcaaactcca tagaaaaata ggctgctttt tttgcatact
    12541 gatcgcttat atgataaggt acttgaaaat ttttaaaatt catctaatgt ttttttagtt
    12601 acaaaatttc aaactttcaa aaattaataa taatggtaga aaatttgaat attattttaa
    12661 ggcaataaaa atatacaaac cgaaacttgt aaaaggtaag ttatacagca ttgttgaaaa
    12721 aaaataaacc cctgatgtta tcaagggttt gtttacaatg tttttaaaaa actattcggc
    12781 ccagctcata gtataatttt cattttctat ttctaatgct tgtttagtaa gcattaatgg
    12841 atgaaatgta tctaacatta ccgctaattc ttttgtttct aatttaccaa tgcttttttc
    12901 aattgcacca ggatgtggtc catgtggtat gcctgcagga tgcaaagtta tcatgcctct
    12961 agttacattt tttctactca taaaatctcc atctacataa tataaaactt catcactatc
    13021 aatattactg tgatgataag gtgcaggaat tgccaatgga tgataatcgt acaagcgtgg
    13081 tacaaaacta caaatcacaa aattattacc ttcgaaagtt tggtgaacag gtggtggttg
    13141 atgtactcta ccagtaatag gttcaaaatc gtgaatacta aatgcataag ggtaacaaca
    13201 accatcccaa cctacaacat caaaaggatg gttagcataa tgcaaaccat acaatattcc
    13261 tttcttttta gtttttataa taaaatcgcc ttgttcatct ttggttatta aattttgagg
    13321 tgccctaata tctctttcac aaaatggcga atgctctaac aactgcccat gtttgctcat
    13381 atatcttttg ggaaaacgaa ttgggctaaa gctttccaca atcaacaacc tattattact
    13441 atcgttgaaa gttatttgat aaatagttcc tcttggtaaa accaaatagt cgccataaga
    13501 aaaactaagt tcaccatatt gggttaataa tttaccgctt ccttcatgaa taaaaatcac
    13561 ctcgtctgca tctgcattct taaaaaaata atcttgcata cttttttgag gagctgctaa
    13621 aaccatgtgg caatcattgt ttacaagtac aggaattcgg ctttgtaaat aatcgttaac
    13681 tggtttaata ttcattcctt caaagcttct atgcttaagc attttctctt ctgcaataat
    13741 aggtgcaaca tctattggag tatctgtttt aattatttgt gttggtgggt ggcaatgata
    13801 taatattgaa taatcatcgc taaatccttc tgtagaaaaa acttgttctg aatataaaga
    13861 accatctact tttctaaatt gtatatgtcg tttaggtgga atatttccag atttataata
    13921 ataagccata aaaaatttga tttataaagt tgaaaaaaaa tataagtatg taaattatct
    13981 gtaaatagaa agttctaaag tagctaaaag aaaaatatat ttccattttc ttttatcaat
    14041 ccagtaggta aaattacgtt ttaatggcat tgagcaaata taaatagaat tattaactct
    14101 attcagttct taatcgcaat gattttataa taaatgcaat tgtttaactg tcgcttgaag
    14161 tacttcttct gtattggaat tgtttttgtg cttccctgta aatgctcctt gctgttttct
    14221 ttgtttaaaa gttttttgaa cgatgtgttt ttgtaattct tctagtacaa ttttcatttc
    14281 ggaagatgca ttttgtaatc tgtcttttgc gtattgaata taatcttctg atatatctat
    14341 accaatatat tttttgttta ataaagaagc agcaactaaa gttgttccac taccacaata
    14401 agggtctaaa actattccat ctgtatcatt aagcattgaa gtaattattc tactgggtaa
    14461 tgttaatgga aaaggtgcag ggtgcgggtt ttttttacca ttttctggag gaaaacgcca
    14521 aatagaagta agcaaagcat gctttgattc taattctatt cctattttat ttttgttaat
    14581 gggtttataa agccagtaaa ttctttcatc tacttgccaa tatctccaac ctctaatatt
    14641 ggctgcaatc attctatccc aaattatttc ttgccttatt gtccaatttg tttttgccag
    14701 ccattgtata ggatgaaaca tttgccctcg ttcccatctt gttttatggt tataaaaaaa
    14761 tgatccgcct tctttggtta ttctaaatat ttcattcaaa atttcgattt gttcttcttg
    14821 atattcctct tctgttttta tatctttata cgaatcatat tgtacatttt ttaccaacca
    14881 gcctttgtgc ttttcttgtt tattgtatgg tggtgaggta atacctatat ctattaagtt
    14941 ggaatctata gtttttaaaa cacttaatgt attacccttg ataatattat tgatatatcc
    15001 tttcattact tgctatctat taaaaattga tagaattttt tattgaagta ttgttggttt
    15061 ctctcaattg catttttatt taagtaaata attttaatta caggataact actgtaatga
    15121 ctaaaattaa aagaagccat tcttgtataa gttccaacaa ttcttctttc tggaagctgt
    15181 tcccttaatt tattataaac tgctttaaaa ggaaattcaa taatgtactg caattcccca
    15241 tctataaacc cacttgacag aaggttaagt ttttctttaa catctttatt taatctttca
    15301 attgtataat cattaaaacc tccttctcca tttaattttg gtttagattt ttttaactca
    15361 tatccttctg tttgaatatt ttttggcttt gcttcgcaag aaattggttt acttccaatc
    15421 gcagagtttt gtttataacc attatagcct aattttttgc tattatgctg ataacctgct
    15481 attgaaactg ttataaattc tcttaaactt gatgagtttt tatcgttaat ataagcagtt
    15541 aataaatcag taagaattgc aattaaattg tctttagact tattcaataa taattcatta
    15601 agcaccttat gtggtttagt tgcataatat gttataattt gatttaagtc ttgattcata
    15661 aaacaaatat aaactattcc ttatcaaata ttttctaaaa aaatgtgtaa aaatttcaaa
    15721 ttgagagtta ttttttctca ataactatgt tcgtaaattt tagttttcca cctctacttt
    15781 cctcagtgca tactaccgtt acatttgtgc taattatgat taaaagagaa gatacatata
    15841 gacacaaggg tttaagaaag caattaattg acatactgaa agaaaaagga ataacccagg
    15901 aaaatgtatt aaatgcaatc aacaatattc cacgacattt ttttttagat cctgcatttg
    15961 ataatatagc ttatgaagac agagcatttc ctattggcga gggacaaaca atttctcaac
    16021 cttatactgt tgcctaccaa acacaattat taaatgtaaa aaaatttgat aaagttcttg
    16081 aaattggtac aggaagtgtt tatcaggcat ctgttttagc agaattgggt gcatcagttt
    16141 ttactattga aaggcaaaag aatttatatg ttaagaataa caaatttatt tttaaaaata
    16201 aataccttaa tattaaattt ttttatggag atggttttga aggactgcct cagtttgccc
    16261 catttgataa aataattatt actgcagcag caccatttat accaactaaa ttaattgaac
    16321 agctaaaaat tggaggacaa atggtaatac ctgtagatga aggtgaagtg caaagaatgc
    16381 taagattaac taaacaaaaa gatggaactt tattagaaga agaatttgaa aaattttctt
    16441 ttgttccaat gcttaagggc aaaaatagtt agaagaatta ttttaaaatt aaagctatca
    16501 ttattgtatg cttccagtaa attttattca aaccatacag catattaatg gctttgatag
    16561 agagggcttc gaagcagttc atactaatag taatcctatt acctccatta gactaaatga
    16621 cgaaaaagtt tgtaataact tatcttattt atatcaacaa caagtacctt ggtgtaacca
    16681 tgcatactat ttaaaagaaa ggccttcctt tacattagaa ccaagttttc atgcaggtgc
    16741 tttttatgta caagaagcaa gtagtatgtt tttatggcat attataaaac aactatatgc
    16801 taataacaca gaaaaaaaag tattagatgt ttgtgctgca ccaggtggta aaagtacttt
    16861 attagccagt tattttaaaa atggtttaat tgtaagtaat gaaataataa agcatagagc
    16921 agccatttta gtagaaaatt tatctaaatg gggtactggt aattttattg ttagtaataa
    16981 tgaccctaaa caatttgcca accttacatc attttttgat gctgttgtaa ttgatgctcc
    17041 ttgcagcggc agtggtatgt ttagaaaaga taaaacagca gtagatgaat ggagtttaca
    17101 aaatgtaaaa ctttgtagtc aaagacaaca aagaattatt gccaatgtaa tgacagctct
    17161 aaaagaaaat ggttatttaa tttactccac ttgctcctat tcagaagaag aaaatgaaca
    17221 aatattagat tttattgctg ataacttttc tgtagaaagt gtagagataa atattaatcc
    17281 ttcttggggt attgtatcta ctaaaagcaa taagcataaa gctatagggt acagattttt
    17341 tcctgataaa ataaagggag aaggtttctt tattgcagtt tttaaaaaaa atgaattctc
    17401 taaaaacaat aaatttagcc ttcaactttt aagcaaatta accagcgtcg aaaatgaact
    17461 aataaaaaat ttcattacaa gtactattga taagaaattt tttaaacata ataatgaaat
    17521 taagtgtata aacactgctt tttatgctga tgtagaaaaa ttagcaaatt tattatattt
    17581 aaaaaaagta ggtacaatta ttggtgaagt aaaaggaaaa gattttgttc ctcatcatga
    17641 attggcaatt agtaacatta gtttaagtaa tattgcaact cttgagttaa ataaagaaga
    17701 cgctttaaag tttttagcta agaaagaatt ggatattaat accaataaag gacttacttt
    17761 aattactttt aatggcttgg gattaggatg gataaaagta ttacataacc gtattaataa
    17821 ttattatcca aatgaatgga gaattttaaa aatataaact tacttagtac caaattatta
    17881 tgaaaaaaat tttctgtata gtacttacat tttttatttt aaaccaatct tttgctcaaa
    17941 ataaaatatt agtgcaaggt tcatacccta atttatactt acaacacaaa gttgctcatg
    18001 gagaaacaat gtatagtatc agtaaattat ataatttttc cagtgctcaa ttagccacgc
    18061 aaaacggtat gacagaaaac gatattctat caaccgataa aattttaaaa attaatttgg
    18121 gcaaaaacaa ttttacacaa gatggacaaa gcggagaaaa tgaaacttta attcccttat
    18181 atcatattgt aaaacctggt gaaacgcttt ttagaatttg ccaaaatttt ggaaaaataa
    18241 gaatagattt tattcgtggg tggaatgatt tagttaatga tattattaag cctgaacaaa
    18301 aaattatcat tgggcattta agagttgatt taaaaaaagc aagcattgta acagaaagaa
    18361 ttattaatac cgatgcacaa gaaaccgcaa ctgcaataga ccaaacagaa ccacctaaaa
    18421 aagatgaaac agaaaccaca actagcacta cacctaagca agaagaggaa gaacctgtag
    18481 tagagcctgt aacaaatggt aacgatgatg aaggtttttt tgttactagc tattctgtaa
    18541 atagcgataa tagtgtatct acaactggcg aagctgcaac ttttaaatca actagcggct
    18601 ggaccaatag aaaatattac gttctatgta atgatatacc tactggtact attgtaagaa
    18661 taactgcaaa taataataga agcatttgtg ctaaagtttt aggtgcatta cccgaaatga
    18721 aagaaaataa aaatttaatt attagaatta gtaatgctgg tgctgcagta ttaaaaatta
    18781 cagagaaaag atttaatgta aaaattactt acaataaata acatttattt cttcaaccat
    18841 ttaaaaggta taggtaaatt attaaaatgc caattaaagt atggttgatt aattgctcca
    18901 aatttttcat aaaatttttt cacaccaggt aaatcacttc cttcaaaatc aaacaacatg
    18961 ttttgattgc ataattcctg taaaatattg ttgtacaaaa aataattggc ttcagttttt
    19021 cgtcctgctt ctgtggttgt gtttaatata ttgtaataac gtttaatatc tttcattaac
    19081 aaagcaatac tcattagttc ttgtttttca ttttctactt ttcgtaccaa aacattatta
    19141 tttttttgta gttgcaataa tagttttttt aagttggtgt aatctatatc tattacatga
    19201 ggcatgttgt tttggttgta caatttatat aatgcaactg ccttttttat gtcatcatct
    19261 tttgtgtaaa taaaatgatg ttttgctgct tttgataaac tgtatgcaat tgtttttttg
    19321 tatttttcct gaattacaga gtaattgttt tttaaatcaa taataaaatt attcttttct
    19381 acaatttttt ctgtaaagtt tttattggaa aaattcaaat taatagagcc atattttaaa
    19441 aatgaatgaa tagcatttat cagttttgta ttattaattt ttacatttcc tattaagcct
    19501 aattgttgtg taaatgcagg catataagca tattgaatac ccaatttatt ctttacagga
    19561 attgggaaaa tcgcttcata atcatttatt atcaaaccgt gccaattctc agccattgtg
    19621 tttaaataaa aactataagc ataaattaaa ccattagaat tttcttgaac acaattatcc
    19681 catttaaaaa tatctatttc agatgatgac aaaattttta tttgatacat aataattata
    19741 atggaatata tctgctaagt ttattcaact gaagctgctg taagtctatg gagaaagaaa
    19801 ttgttttaga aaatcttttt gaacctaatg tagatttaaa ataatctgca tataccttac
    19861 tatacaaaat aggtatataa atatttacta accctttaat caaaggcagt tgtaaacctg
    19921 catcatataa aaacctgcct gtcgctgggt tttctttcca agattcggca taagtgccaa
    19981 tatctacaaa aaactttaaa ggaattttta ttggcaaaac atttaaaata ttaattgcat
    20041 caggaatatt accacttaaa tttaaagcca ttaaccaatc atctgttttg ccaatcttat
    20101 tacttaacaa atctgtactt accttaaaaa aaccatcacg ttgcattatt tgttggcttt
    20161 gccaaccttc aaattcattt ctgccaataa aataattact gtaagtataa tcttcatacc
    20221 ctttagcacc actcatgttt aaatgatatc tatctgtttc gtactgttta ataaatgttt
    20281 tatcggaggt gtagaagaat tttccggcaa aaaatcttgc atcaattccg ccctttctat
    20341 ttttataatt gaaatgataa tttgccgtaa aaccagcacg aacaaagctt ttaccttggt
    20401 ctatggttaa attaaaattg taaggataca gtattctgtt atctaatgtt gttattttaa
    20461 attgatttat ataactgttt tcagttgttt tttctacaat atcaacacca tttaacgttt
    20521 taaacaacaa gccattttct tgtaataaaa aacttcttgc ttgaaacacc caacgcagtg
    20581 tacttctttt atcattgcga tacaaagtgt atttaactga tggaacaatt tttacaactt
    20641 gttgattaat tgttgcagca ttagttggtt taaaattatc tattgtaaat tttgatgcac
    20701 tcgtagcaaa ttctagccaa tatttttctt tataagtatt aaaacttgct cttgcattaa
    20761 agttaaactc cttactgcca aaggcataca aaggtgcaac aaaaaattga aaccgattga
    20821 gcggtaactg ataattatga ataaaagcac caatagaaac cttatcgtaa taattatatg
    20881 cagccaaagg cgaaatacct atataattat acttttctgt ttcttttaaa gaggctataa
    20941 aaatcggttt aattttttta ttataactta ctgtatctaa atttttttta ttgtataaat
    21001 aagaaaacaa aaactctgct tctttgtttt tattaataac tattttttta aaatcttcag
    21061 gataaggatg tttaaatttc caatcattat aatattgttg cattacttct ttaaaactat
    21121 cttctcctaa agttgtttct aattgtttca tccaactgct tgccttagaa taaacaatta
    21181 aaaaataatt aaggttacta taagctgtac taattgtatc aattggttga gattttttaa
    21241 ttttggtaat agttctattg gctacttttt caagcacatc tatagaataa ttgctaagca
    21301 aacttttatt attactagca tattgttcct tcatatattt tttttcataa aaagtattca
    21361 tgccttcatc catccacgca tgttttcttt cgttgcttgc caacaccccg taaaaccagt
    21421 tatgtccaat ttcgtgtaca atagtttcgt ctaacctttt cccttcagta tttgttgtaa
    21481 ttacagtaat tgtaggatat tccattccac cactaaattc attttcaaac ccagcaacaa
    21541 cagatactgt attataagca tattctccaa cccaattact atattttttt ataccatttt
    21601 tagcataagc aatactctta ttccatgctt tcaactgctg tggtaaataa aaagtaaata
    21661 catctacagt tttagtagta agtttaacag tatcatattg cagaataaaa tttttcgaag
    21721 caaaccatgc aaaatcatgg caattagttt gtttaaagtt ataggcagtt aatgttgggc
    21781 tatgtttggt agctgttact ttagaggttt tagagggaga tatttttttt tgcttttctt
    21841 cctttaaata attcaaataa ttggcttgtt tttctggttt ttcttttcct agtttcttta
    21901 aagtatgtaa caaatcagtt tgttgtaact caccagttgc agcaacaata taagaatcgg
    21961 gtaaaataat ttgaacatca aaatctccaa attcactata aaattcgcct tggtctaaat
    22021 aaggcatttc gtgccaacct tttctatcat aaactgctgg cttaggatac cattgtgtaa
    22081 taaaataatt ttgcttactt acacccattc ttgaaaacaa agcaggaatt tttacaaaaa
    22141 agggagtagt aatggtaata gattgctgag gaggtaatgc acttggtaaa attaatttta
    22201 ttatatcggt atattgttga tgcacttcag ttaatgcagt tacattattt actttaaagt
    22261 ttagctgatt aatatagcct ctatcctcat cattactaaa ataaaatgtt gtacttccgt
    22321 tctctaatgc ttgtttagta taagcagttt tatcattttt ataggcatta gcccataaat
    22381 gaaaccaaat aaattgtaaa gtatctggcg aattattata ataagtaata gtggcaaaac
    22441 catctaaagt atttgcagta tcatttaaac taacttttaa attataatct actttttgct
    22501 gccaataatt gttttgcgag aaggaattta ttacacaaaa caacattagt aatagtagaa
    22561 caattctatt tttcaccctt ctaaaaattt ggttgaagat atataaatat tttacacaaa
    22621 ataggctatt caaatcaaac tataattatt agtaaacata ttagttattt ctgtttaaca
    22681 ttgcaacata aatcagtagt atgtacccta atttatatta tttttttaaa gaagtttttg
    22741 gtatagaaat agaaggcttt aaaatcatca attcatttgg cttttttgta gcaatagctt
    22801 ttataggtgc tgcatgggct ttaacacaag agcttaaacg taagcaaaaa caaggcttat
    22861 ttgtttatac cgaagaagaa ataattgtag gcaagcctgc ctctgcatct gaattaatga
    22921 tacatttttt cttaggtttt atatttgggt ttaaaatatt aggaggcttt tttttaccaa
    22981 atgcttttag cgatccgcaa ctatatattt tttctggtca aggaaattgg tggatgggtt
    23041 tattagttgg tggcgttttt gcatggataa aatacaatga aaaaaataaa ctaaaattac
    23101 ccacacctga aaagagaact attagaattt ggcctcatga tcgtgtaggt gatttagtaa
    23161 tttatgcagc catatttggt tttgctggtg ctaaattatt tgatattcta gaagccccat
    23221 cagcattttt ttctatgttt aaagcggtaa aagaaggtaa acaagatgta ggctctttac
    23281 tatttagtgg gttaactttt tatggtggtt taattgttgc caccattgcc attattgttt
    23341 atgcaaaaaa acataaaata ggcattattt atttagccga tgcttttgca cctgctatga
    23401 tgttggctta tgctttagga agaattggtt gccaggttgc tggtgatgga gattggggta
    23461 ttgttaacag tgcttttatt agtaataata ttggagaaag cattttagca acacccgaac
    23521 aatttacaaa tgcattacac actcatagtg cttatttaag cagtaagttt ggaacaattg
    23581 aacaaatgca ccatatgagt attcagcctt ttttaggttt accaaattgg atgtttggtt
    23641 atacttatcc tcataatgtt gtaggcgatg gtgttgcatt agccaactgt tccggtgtac
    23701 attgcaatta tttacccctc cctgtttttc caactccttt ttatgagtta acagcttgtt
    23761 taattttatt tttaattctt tgggcagtaa gaagtaagtt aaaaatacca ggacagctag
    23821 caggcttata tcttattttc aatggaaccg aaaggttttt tatagaaaaa ataagagtga
    23881 atgcaaccta taatttagca ggaatgaaaa taacccaagc cgaaattatt tcattaatat
    23941 tagtaattgc aggagttgtt ttactcacaa tgtctaaaaa atggtttggc aaattagcaa
    24001 aataattttc ctaaaaaggt tattaatcta caacttattt actttggcta caatacctta
    24061 aatttgtagg ttatttagaa taaataaagt aataacatca ttaataatta aatacttatg
    24121 tgtggaattg ttggttatac aggtaaaaga caagcttacc ctgtagtttt aaaaggttta
    24181 aaacgtttag aatatcgtgg atatgatagt gcaggtgttg cactaaaaaa cgaaagagga
    24241 ttacatattt ataaaaagaa aggtaaagtg gcagaaatgg aagaaagtgt tgttggtaaa
    24301 gatgtagatt ccactattgg aattggccat actcgttggg caacgcatgg tgaaccaagt
    24361 gatagaaatg cccaccccca tcaaagcaat gatggaaaac ttgccatgat acataatggc
    24421 attattgaaa attacgcttc gctaaaacaa gatttaataa ataaaggtta tactttttct
    24481 agcgatacag atacagaagt tttattgaac tttattgaag atattcaaaa aaacaataac
    24541 tgtcctttag aagaagcttt aagaattgca ctgaaaagaa ttgtaggtgc atattgtatt
    24601 ttattaatta gcgaagacga cccagaaaca attattgcag cacgtaaagg cagccctctt
    24661 gtaattggta ttggtaaagg tgaacatttt ttagcaagcg atgcaagccc tattattgaa
    24721 tacaccaaag aagtagtgta tgtaaatgat tatgaagttg ccattgtaaa gcccaatgaa
    24781 cttattctaa aaaatttagg caacgaaaaa caaactcctt ttattactaa attagatatg
    24841 gaacttgctg ccattgaaaa aggtggttac aaccatttta tgttgaaaga aattcatgaa
    24901 cagccagata ctatttatga ttgtttaaga ggtagattat tgccagatac aggacaaata
    24961 atgatgagtg gaattgaaaa caatatagat gcattaaaaa atgcacaaag aattgttatg
    25021 gtggcttgtg gcactagttg gcatgcaggg cttattgcag agtatatgat agaagaattg
    25081 tgtagaatac ctgttgaagt agaatatgca agtgagttta gataccgaaa tccaattatt
    25141 cataaaggag atgttatagt tgctatttct caaagtggag aaactgcaga tactttagta
    25201 gctctagaaa gagcaaaaga acaaggtgca tttatttttg gtgtagttaa tgttgttggt
    25261 agtagtatag ctcgtataag ccatgcaggt gcttacacac atgctggccc agaaattggc
    25321 gttgccagta caaaagcatt tactggtcag ctagcagttt tagcaatgat ggctttaaaa
    25381 attgcaaatg ccaaacaaac aattacacag gatagatata ttcaattaat gaaagatcta
    25441 cacaacattc ctgaaaaagt aaaagaaatt ttagcagata catctactat tgaacgcatt
    25501 gcagaaaaat acaaaaatgc ttccgacttt ttatttttag gtcgtgggta taattttcct
    25561 gttgctttag agggtgcttt aaaacttaaa gaaatttctt acattcatgc agaagggtac
    25621 ccagctgcag aaatgaaaca tggcccaatt gctttggttg atgaaaacct gccagtagtt
    25681 tttgttgcaa caaaagattc gtattacgaa aaaattgtaa gcaatgtgca agaaattaaa
    25741 gcaagaaagg ggcaaataat tgcagtagca accaatgatg atactgttat accaggtatg
    25801 gctaatgatg taatgtttgt accagataca gatgaagtaa tagcaccatt attaagcgtt
    25861 attccattac aattattaag ttattatgtt ggtgtagcca aaggtttaga tgttgataag
    25921 ccaagaaatt tggctaagag tgtaacagtt gaataaatat ttttattttt aaattatata
    25981 tagaaaattt tctgtataaa aatttcaact ttaatacatg cctcaaaaca atattaaaaa
    26041 aagccattta tcctctttgg gttatggctt tattaaaaat gagcatatac caaaaggtaa
    26101 agatgaatat tacctaagag atttacaaaa caaaagtggc attaattatc gccaacttac
    26161 tgcttatgaa attgaagttt tagtacgcaa tagaaataca agcgataact ggaacaatat
    26221 tttagtaagc aatgctttta acccagaatt agttaacgac tgcaaatttt atggcttagt
    26281 tagaatagga aaactagagc ctttttgttt agaatacagc gatttaaaag tgcctgttgg
    26341 tttatacaat tcaaccatta ttagctgcga ttttggtgat aatgtttgta tagataatgt
    26401 tagatactta tcgcattata ttataggcaa cgaagttatc attgtaaatg ttaatgaatt
    26461 aaccactact aattttgcaa aatttggcaa tggaattatt aagcaaaacg aaacagaaaa
    26521 ccgaagaatt tggttagaaa tttgcaatga aaatgcaggc agaagtattt taccttttaa
    26581 tggcatgtta actgcagatg cttttttatg gagcaaatac agagatgatg tagaattatt
    26641 aaacaaattc aaagaattta ccgaagcaag atttgataat agaacaggct attacggaaa
    26701 agttgggcac agaactgtga taaaaaatac tgctattatt aaagatactt ggataggtaa
    26761 tgatgcatac ataaaaggct ctaacaaatt aaaaaaccta accattaatt caggagagga
    26821 aggcaaaaca caaattggag aaggttgtga attagtaaat ggtattatag attatggatg
    26881 tagagttttt tatggtgtaa aagctgttcg ttttataatg gcttcacact ctcaattaaa
    26941 atatggtgcc aggttaatca attcttactt aggtaacaat gcaaccattt cttgttgcga
    27001 ggtattaaac tcattaattt atcctgccca cgaacaacat cacaacaact catttttatg
    27061 tgcagcacta attatggggc aaagtaatat tgcagcaggt gctaccattg gctctaatca
    27121 caactctaga gctgcagatg gtgagatagt tgcaggacgt ggcttttggc ctggcctctg
    27181 tgtaagttta aagcataaca gccaatttgc atcttatac
//
LOCUS       DAOQPK010000005        24355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_976_length_24355_cov_4.958272, whole genome shotgun sequence.
ACCESSION   DAOQPK010000005 DAOQPK010000000
VERSION     DAOQPK010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 24355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24355
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_976_length_24355_cov_4.958272"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..1816
                     /locus_tag="PLU36_00535"
     CDS             86..1816
                     /locus_tag="PLU36_00535"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HMZ45266.1"
                     /translation="MKKLLLALVIHCSVVNFSIAQDDMKSVWSIKLEHKAELNEFDEA
                     SGILVSSNEKEISIVDATTGKVKWTKDFKTLCDGIMKKVDERIPMWEAKCIFLFDRKS
                     GKDQMAVIDIETGQMLWSSNKYEGVTEASIAYVPEVGMFAIANKKAFSMVKARTGEEV
                     WETQGFKGTLAKYIYNASDNSIIALNYNPAAAGEGILGGMGKLFAGFGAFKSQLTKFS
                     VKNGDILWQTDIKGAVEKEIATRKILAKLKIVDNDKLMLIMQGMQMFDLNNGSLLWGV
                     THSEEVLKESKRGIPSGYSGSKLVKSAVYGAVADPLIDGNDVYLFDMQNKKNQYVSKY
                     DLHTGKLIWKSKELKKLTIAPSLYKVGGKIIIQIGGWAQVQGIVEQKSNYGGGFGGSG
                     FSVSTYKRVKFYKEFSPYGVEALDATNGELVWRSERFSKGVTNSFVHNGELVVASGKE
                     LYNLNPDSGTEKYSESVKDDGIKETEQIFKVGDNVVVVCSKGMSNYVIADGKKNWTVK
                     TKKGDLSTVNNGMAFYITEKNDFVVIDLTTGKYTTYDARKDSDSEIYEDGQYILVFEK
                     DKVTKLATKQ"
     gene            complement(1998..3176)
                     /locus_tag="PLU36_00540"
     CDS             complement(1998..3176)
                     /locus_tag="PLU36_00540"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HMZ45267.1"
                     /translation="MLDILKEMPWVTAIILTAWLPVIIITLLHLINRKDISITRKIVW
                     LIIGLIPIIGLLIYGLINYKVKKSIVWLTVLATIISLITVWYFIFYQPSIAKRDVTKE
                     AAIALSVTSLLKEFEVNETEANIKYTNKVIEVSGVVEKILNDETGMVIFLQTNIEGKS
                     FSGRLKAKQATQENATITLKGILTGYILGQIQLNEAVITNGANTALSNEPTSTKDSTI
                     NLKVPIKKDSIKVVQPTEKIYSSSKGLIKFISITAVEDIEASNNQVISTISSNGKVQF
                     AALIKGFLFENELMQKHFNEDKYMHSDKFSKSEFDGTITNINSINFNTTGKYKVNAVG
                     NLTIKGITKKITTEGTIDVNNNTILLHSIFKLKIKDFGIITDEIAEQIDIVVSATYKK
                     "
     gene            complement(3192..3584)
                     /locus_tag="PLU36_00545"
     CDS             complement(3192..3584)
                     /locus_tag="PLU36_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HMZ45268.1"
                     /translation="MAFKINWDALGISTSIACAIHCAVLPLVVTSLPLFGINIIHNLW
                     FEFSMIGIACCIGIYSLYHGYKKHHHSFFPIFVFITGIIFLFAKQIWHEFEIWLLIPA
                     VIFIVTAHFINYRSCRIHKHAHKDDCNH"
     gene            3670..4326
                     /locus_tag="PLU36_00550"
     CDS             3670..4326
                     /locus_tag="PLU36_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HMZ45269.1"
                     /translation="MMKKKKWIFYVTFFVGLILVFWLALYLFTDTFNQSRLVNRSVVK
                     PFTFYKEDGSLFTKADMAGKVCVVEFFFTTCPNICPEMNTNMKKVYEAFKDEPNFLIL
                     SHTCDPKRDSVPVLKKYADSMQVNTQVWAFLTGSKDSLYNMARESYGIDDPKSVVNNI
                     EDDFLHSQFFALVDKNGNVRGQVYDGLKQDEIKQLKKDIKELLNDKGNRRGFSNNLFN
                     "
     gene            4362..4781
                     /locus_tag="PLU36_00555"
     CDS             4362..4781
                     /locus_tag="PLU36_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HMZ45270.1"
                     /translation="MQTEFLSILKKNNLSVTESRMKILELFAQANGALAHNDIEKKSK
                     LQFDRVTIYRTLQTFVEKGIIHTIPTADNSVLYALCKDECTQGHHNDNHVHFICDSCN
                     VTYCLEHSNSPSVILPKGFKQNRVDVVVSGICLKCNA"
     gene            4785..5339
                     /locus_tag="PLU36_00560"
     CDS             4785..5339
                     /locus_tag="PLU36_00560"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMZ45271.1"
                     /translation="MILVTGATGLLGNELIQQLITQGKKIRALFYHTNPTLLHENIEW
                     KQVDILDIVDLEEAMTGITQVYHCAALVSFNKAQKNYLHQINIEGTKNIVNACLQEKV
                     EKLLYVSSVAALGRIREDKPIDETMNWTEETSNSEYGKSKYLAEMEVWRGIGEGLNAV
                     IINPTIILGASNWSKGSTEIFKTA"
     gene            5418..5756
                     /locus_tag="PLU36_00565"
     CDS             5418..5756
                     /locus_tag="PLU36_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45272.1"
                     /translation="MNSNINAERFILSSETMTYKDIFSTIAKAFNKKPPYKKVTPFLA
                     ALVWRWEAIKGKLTGKHPLLTKETAKTAQTKASFNNSKLLNFLPEFKYNKIDETITRI
                     CCELKAMYKL"
     gene            complement(5759..6226)
                     /locus_tag="PLU36_00570"
     CDS             complement(5759..6226)
                     /locus_tag="PLU36_00570"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HMZ45273.1"
                     /translation="MHSNKIEFIVQSFPALLKTANENSKPLWGKMNLQQMVEHVTEFF
                     TISTNPLQQNIITPVDLLPKYKAFILSDKEFKENTQAPVELIPVEPKPLINASIEIAI
                     NLLTQEITKFVQYFHLHKNAQTIHPVFGSLNFDEWVLLHYKHVLHHCKQFGLV"
     gene            complement(6245..6904)
                     /locus_tag="PLU36_00575"
     CDS             complement(6245..6904)
                     /locus_tag="PLU36_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMZ45274.1"
                     /translation="MLAKQIIQTNFPIIQLNDKVIVALRILDDFDIQHLPIISEDKFV
                     GIISKDDLLDADENNIIATLQNYFNTAFVKTEEYFLSAIKLAAQYQLSAIAVVNEQIE
                     LQGIIPLNELVNATALYLSCNEPGGMIILETEKRHFSFGEISRLIETNDSYITQLNTY
                     VEQDTGLFIVTIKVNTIEISDIVATLQRYDYTIRYYFGEEQYNNELKENLNQLMFYIN
                     M"
     gene            complement(6947..7705)
                     /locus_tag="PLU36_00580"
     CDS             complement(6947..7705)
                     /locus_tag="PLU36_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ45275.1"
                     /translation="MNYTIHQQDKFKYLEEGDGEPLMLLHGLFGALSNFADLVEHFKK
                     THKVIVPMLPLFELDIFHTTVGGLQKFVHKFIEARGYNNIHLLGNSLGGHVALVHVLK
                     HPEKIKTLTLTGSSGLFENGMGDSYPKRGDREYMRKKTELTFYDPAMATEELVNEVFE
                     ITNNRLKVIKIIALAKSAIRNNLGEELNQIKQPTLLVWGNNDTITPPFVGQEFNKLIP
                     NSELHFIDKCGHAPMMEVPLEFNKILDTFLSKNK"
     gene            complement(7885..8421)
                     /locus_tag="PLU36_00585"
     CDS             complement(7885..8421)
                     /locus_tag="PLU36_00585"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HMZ45276.1"
                     /translation="MLIDIIVLVLLILAIFKGYTKGFIVAIFSTISIFIGLLVALKLS
                     AVTANYLQQSNIKIPIKWLPFLSFMLVVVATIILVRVGSAIIEKTVEFALMGWLNKIA
                     GIALYILLHFSVLSILLFYLIEMNLLSANTVQQSKTYFFIQPIAPKAIQVIGSITPMF
                     KNIFEQLEIFFSSIASNA"
     gene            complement(8451..8903)
                     /locus_tag="PLU36_00590"
     CDS             complement(8451..8903)
                     /locus_tag="PLU36_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HMZ45277.1"
                     /translation="MSLEIQIMGEMKEAMKAKNEAVLRGLRAIKAEIIKAKTEPGANG
                     QINEDTELKLLQKLVKQRKDSFEIFTQQNRADLAQKEKEEIEVIEKFLPKQLTEAELE
                     QVITTIITNSNATSAADMGKVMGIATKQLAGKADGKVISTIVKKLLSV"
     gene            complement(8910..9257)
                     /gene="gldC"
                     /locus_tag="PLU36_00595"
     CDS             complement(8910..9257)
                     /gene="gldC"
                     /locus_tag="PLU36_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HMZ45278.1"
                     /translation="MEKSTITVDVILDENKIPEQISWKASNSSAENKQFAKAVLLALW
                     DGAEKTALRIDLWTKDMMVDEMSDFYYQTFMGMADTYGRATNNQEMVNDLKNFAKSFY
                     DKFRDQQLKENKL"
     gene            complement(9296..10420)
                     /locus_tag="PLU36_00600"
     CDS             complement(9296..10420)
                     /locus_tag="PLU36_00600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ45279.1"
                     /translation="MRIVINTKMLPFNNTCIYRNYMQVMINKLVSRYKHYQFYIITED
                     ENNFEITEAENCIVKIISLKSTNSIALSYWYNIKLATFIKKVKPSIVWHLDSRCIVGL
                     KTPQIITIAHLSFLYQTNNLSNTARIMYSLYQKKWIQKASATITFSLFTQKAIFENYR
                     INNKVEFISPVISSTYNPITYVEKEKIQQTLTGDEEYFLYFGSFNTQHYLVQLLKAFT
                     LFKKRLQSSMKLVLIADAFSNTTILKEKLKTYKFKEDVLLIENASEELLQKITSAAYA
                     FIYTYEHTSIAFNALQAMQAGVPIACSNTGCLAELFNNVALFFDTNNEKNIAEQMMML
                     YKDEGLRNNLIENGIKKAQEFNWDKSINETITIIEHYAMP"
     gene            10544..11575
                     /locus_tag="PLU36_00605"
     CDS             10544..11575
                     /locus_tag="PLU36_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HMZ45280.1"
                     /translation="MAYFNLTDTLNFLSKLTFRRAWNAYKVWLSFQISKYSGKPIQWG
                     YPISISFEPTTSCNLRCPECPSGLREFTRPTGMLQNSFFKKTIDEIHKELLYLIFYFQ
                     GEPYLNPNFLEMVKYAHDKGIYTATSTNAHYLTEEKAKKTVESGLDRLIISIDGTTQD
                     VYQQYRRGGKLEKVLQGAQNIVKWKKELNSKTPFIFFQFLVVKPNEHQIEEVKKLGKE
                     IGVDQVRFKTAQVYDYENDPNNLIPTIEKYSRYKKDKTGSLKVKSGMHNYCWRLWHAN
                     VITWDGLVVPCCFDKDAMHRLGNLQTQNFKDIWHNNNYKHFRAELMKSRKNIDICANC
                     SEGLKVWED"
     gene            11690..13780
                     /locus_tag="PLU36_00610"
     CDS             11690..13780
                     /locus_tag="PLU36_00610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ45281.1"
                     /translation="MKKSLLLGLLFLISTIGWSQTISVAQADKAGSSSNVISGAGAYH
                     ALEAIYLDAEIGASNFIGVGNEIQKIRFAANTNSTGTFPGSIAGYSIYMKNVAAGTKT
                     FTAGSYSLSGYTLVYTGALSATSAGAPYSFTFDAEVTLTTPFARTAGNNLQVLILRNS
                     GIAATGYAWDCSNGNSVDGGTANTSRRYNGATQAVENSTSLSVSAFRPAIQLIRPVSN
                     DATVTVDGATLPTASCFSTPQTVIAYLKNSGTSTIAIGGASVKIDVTGANTYTTTVNN
                     TGALAANASEMIIFSGINLNNAGVNNFKIYSTMAGDGNATNDTAAFTITTTATYNTYP
                     VNTSAETNNLVFQYLKTLNVSNHWTLNSAITASATGAFKNTDLTDSIFPKTGTRFYVQ
                     DNYSGANSTGHSSVLYAGCFNFSSPANISFWMTQDNSLNTSLDSIYVVVSTDKAATWT
                     RLQGFGRLSASALLPTWVQQTVSLNSYAGQTIHIGLEGVSKWGNVIGVDDISVTANAP
                     VPVTFGEFVGVRENNSNQLKWNTYTETNNQGFELQRSVNGTEFTTIAFVPTKAINGNS
                     NSELNYTFQDKASSATTYYQLKQLDKDGKYAYSKVVSIKGEKPTRFELANLYPNPAKE
                     TIKVTVAAPKAAVVTLTVTDMTGKVVMKQNNQLTMGDNNIALNVAALNAGNYIVRLSC
                     ADGCEYSIQRFVKQ"
     gene            complement(13894..14727)
                     /gene="murI"
                     /locus_tag="PLU36_00615"
     CDS             complement(13894..14727)
                     /gene="murI"
                     /locus_tag="PLU36_00615"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HMZ45282.1"
                     /translation="MHKGAIGIFDSGYGGLTILKDIEQKLPEYSFIYLGDNARAPYGS
                     RSFETVYQYTWQAVQWFFSQGCHLVVIACNTASAKALRNIQQNNLPSLSNNLKRVLGV
                     IRPTAEVIGNYTNTNNIGILGTTGTIQSKSYILEIEKFFPSIKVYQQACPMWVPLIEN
                     NEYNSKGADYFVKKYINQLTEQSADIDSILLACTHYPLLINKIREYAPADMAIVTQGE
                     IVATSLSQYLNRHQQLDAICKKEGSTNFFTTDDPLMFNKQASIFYGKAVESNYVSLHL
                     L"
     gene            complement(14873..15472)
                     /locus_tag="PLU36_00620"
     CDS             complement(14873..15472)
                     /locus_tag="PLU36_00620"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HMZ45283.1"
                     /translation="MKKVFMACAALVISLAATTKSNAQQIKIGMFDEEAVLQFMPGIS
                     KVDTLLHQFVEDSLQNEYEDELYLYQTKDSTFKADSSKMSNSLKLTLKKEIALHFNKL
                     QNWQQYQQQRLQIKQQELLRPYLEKIYGALQAIIIEQKYTHIFKKDVFVHIEKGEELM
                     LRVLHKLKVPVPKEIEEQYKSFGIGGASATKPTTTPKKN"
     gene            complement(15507..16019)
                     /locus_tag="PLU36_00625"
     CDS             complement(15507..16019)
                     /locus_tag="PLU36_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HMZ45284.1"
                     /translation="MKKIILLLVCTLATTIFINAQRYAIIDTKYILDKIPEYKNADRK
                     LQSIAEQWQKEIDNKQAELDKMYKDYEAEQFMLSDDLKKKREDEIFNKEKEVRELQKK
                     RFGYEGDLFKERQKLVKPIQDRVYTAVQKIAVNRGYDFILDKSEGITVIFADPKIDKS
                     EDILKELGIK"
     gene            complement(16051..18804)
                     /locus_tag="PLU36_00630"
     CDS             complement(16051..18804)
                     /locus_tag="PLU36_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HMZ45285.1"
                     /translation="MSKRLSTKYLLLLFLTAFSTAQLSAQQNNKDTTITSVDAELINL
                     FNQKKPQKYKIAGITVVGNNYFDETLLVSISGLNIGDEIIIPGGDNFAKAIQKLWAQN
                     YFSDVAIYVSKLVDKSIWLEIHVTERPRLARFTFVGIKKSDKEDLIGKTGLVPNRIVT
                     ENMKKNAEDAIIKFYAEKSYRNVTVTIDEKKDSIINNAIELVFHINKGNKVKVNNIDF
                     ANVTLDPLKLKKQLKDTKEKSRFTIKKSDDKSAFSGITKYTFKDYLNENGFLTYSKTK
                     KLLDPYIRFKFLSSAKFNDKKFNGDIEKLLEYYNTNGYRDAVINKEDIKILPNAKGDL
                     NISIKVNEGKKYYFGNIAWRGNTKYSDSVLTSILGIKKGDVYNLETLKKGLGIQLSPE
                     GGDVSGLYMDDGYLFFRTEPIETAVYNDTIDYLIRVTEGPEATIKDVNITGNNRTKEH
                     VIRREIRTIPGEKFRRSDLIRSQREIAQLNFFNQEKIGINPRPNPEDGTVDIDYSLEE
                     KSSDQLELSAGWGGGIGLTGTVGVSFNNFSIKNIFKKQAWDPLPMGDGQKLSLRVQSN
                     GRAFRSYNFSFTEPWLGGKKRNAFTISVFDTKFSNAYDPLTGTYTSNAANKSYIKTSG
                     ASVSLAKQLKWPDDYFSLSIGLSFSRYKLKNYYIDQVNLPGFNNGFSNNLSLKFALQR
                     SSVDNPLFPRQGSNFLASLQLTPPYSLFDKTIVTSVNPYQWVEYHKWRFNSEWYVPLT
                     KAVGPEKNKQLVLKAAAKFGFIGKYNNKLKVSPFERFQLGDAGLSNQFALLGYDIIAQ
                     RGYPVYQTSNPKVNPDQQGASEYFTIFNKYVLELRYPLSLNPSSTIYALGFFEAANGW
                     FSMKDYNPFQLRRSVGIGMRFYLPMFGLLGFDYGIGLDRITPNGKLKDAAKFTFMLGF
                     EPE"
     gene            complement(18977..19753)
                     /locus_tag="PLU36_00635"
     CDS             complement(18977..19753)
                     /locus_tag="PLU36_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HMZ45286.1"
                     /translation="MQPLVDKINKQRLPKHIAIIMDGNGRWAEEKGEDRLFGHFHGVE
                     SVRNIVEGCAELGIQYLTLYAFSTENWDRPQQEVDGLMTLLVDTIRKEVPELNKNNIK
                     LHVIGNTSMLPELARNELNEAIDLLKNNTGLNLIMALSYSSRWELINAVQAIAKDVEM
                     GNLNPNSINNTVFEKYLTTANFPDPELMIRTSGEFRISNFLLYQLAYSELYFTNTCWP
                     DFRKNNLYEAIIEYQGRERRFGKTSKQIHDNIVLNSTSHP"
     gene            19933..22656
                     /locus_tag="PLU36_00640"
     CDS             19933..22656
                     /locus_tag="PLU36_00640"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HMZ45287.1"
                     /translation="MRQFLIIAFLVLTTNIQAQFTVTPNNNAMQLAQKIVGQGVQIVN
                     AWFKGSSTSAGFFTDNTGTLGISSGVLLTSGKAANVAAAASSQNDATFGAGDVDLTNL
                     VGTGTGNDACVLEFEFIPVGDSIVVKYVFASEEYPEYACSDFNDVFGFLLKSPLPIFP
                     PNVFKNIALVPGTTIPVKINTINPGAGVNGQIGNCTSQGTGSPFTQYYRNNLSNSYII
                     YDGMTTILVAKAKVVPCQINRIKIGVQDMVDGVFDSGVFIEANSFRSDTTSLIKTGGY
                     TDAQGNKNVAVEGCSKVKVKITLEKPAGPSGKTLQLNYSGSATRGVDFDAALAPTSAV
                     FAAGQSDFELEIKPIVDAVVEPLDSVIISAGIACATSTAKITVYIKDSITFRNSKDTF
                     TCSSFPTVLTASYDSSNTNVYSWNVTPAVTTQTLSVTKPGVYIATNTFLNSCYNVDTF
                     KVVKGDPDLFLGNDTTFCSKDSIILTPIKSPYGGSFTWQNNATDTFQVVKNTGTYWTK
                     YTTPNGCYVYDTVEITKVQMPYPQFGNDTAFCANSSVTLTATNYTGATYLWNTGATTN
                     TINVNSSGTYSIISTIGLCESKDTINVIKSDLPIKNLGNDTTYCGNNGIVLNATYPGA
                     TTYLWNTGATTATINITSTGTYSVTNNLNGCIAKDTIKITINPIPYPQMLISDTALCP
                     YENINLNAFYPGATYLWNTGETTNIINAKTGGLYIVTNTLNGCIAKDTVNIKNNKAAI
                     AYAGNDVLMYAGSSVNLTASQNANNKTYQWTPLNNLSSPNYYTTNASPKDTTDVFYYL
                     KVTSVDNCVAYDTILVKIMNAKFDIPNAFSPNGDGINDLWEVPFLYSFPTSRMQIFNR
                     NGQIIYTSGGTNYKPWNGMYNGKPAPIGVYYYVIEVGNGFPRKTGWVAIVR"
     gene            complement(22731..23504)
                     /locus_tag="PLU36_00645"
     CDS             complement(22731..23504)
                     /locus_tag="PLU36_00645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ45288.1"
                     /translation="MQKISAAIITFNEEKNISRCIESLLQIADEIIVVDSLSTDNTKN
                     ICSKYPIKFIEQPFLGYIEQKNFAVALTYFDYVLSLDADEALDETLQQSILAIKQKKV
                     VADSYKMNRCTNFCGKWIKHGTWYPDKKIRLFNKHNAKWAGTNPHDFVEIDKQSSCIY
                     IKGDILHYSYNSLEEVITQANKFTTIQANAMYKKGKRASVINLIVNPCVAFISGYFIK
                     LGFLDGKDGFILAKTIAYQTLLKYAKLLHLQKANKHNNI"
     gene            complement(23530..24246)
                     /locus_tag="PLU36_00650"
     CDS             complement(23530..24246)
                     /locus_tag="PLU36_00650"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ45289.1"
                     /translation="MQTKVTKQFNPPLTSAYYFIRKLLLHKIIEYKNHLQGNLLDFGC
                     GSKPYKSLFNHVSSYTGVDYENTGHSHINEQIDVFYDGKNIPFKDNEFDSILCSEVFE
                     HVFNLEEILPDLNRVLKPGGKILITCPFVWNEHEVPYDYARYTQFALKHLLEKNGFKI
                     IVLDKSGDFATTLHQMKMVYISEHFLQSIFIIGKLKFITSNIRSPLMFLCNGLFLLLH
                     KLMPKRNDLYLNNIVIAEKK"
BASE COUNT         8276 a         3648 c         3768 g         8663 t
ORIGIN      
        1 attaaaaaac aagagtttga tttaaactta cctaaaaata aacgattaaa atttagatat
       61 acttttcaat tataaaataa aacacatgaa aaaattatta ttggcattag taattcattg
      121 ctccgttgta aacttttcca ttgcacaaga tgacatgaaa tcagtttgga gtataaagct
      181 tgaacataaa gcagaattaa atgaatttga tgaggcatct ggcattttag taagcagcaa
      241 tgaaaaagaa attagcatag tagatgctac tacaggtaaa gtaaaatgga cgaaagattt
      301 taaaacactt tgtgatggca ttatgaaaaa agtggacgaa agaattccga tgtgggaggc
      361 aaaatgtatt tttttatttg acagaaaaag cggtaaagac cagatggcag ttatagatat
      421 tgaaacaggc caaatgcttt ggagtagcaa taaatatgaa ggtgtaacag aggcttctat
      481 tgcttatgtt ccagaagttg gaatgtttgc tattgcaaac aaaaaagctt ttagtatggt
      541 aaaagcaaga actggagaag aggtttggga aacacaaggg tttaaaggta cgttggcaaa
      601 atatatttat aatgcaagcg ataattctat tatagcatta aactacaacc ctgccgctgc
      661 aggagaagga attttaggag gtatgggaaa actttttgca ggctttggtg ccttcaagtc
      721 tcagcttaca aaatttagtg taaaaaatgg agatatactt tggcaaacag atattaaagg
      781 agcagtagaa aaagaaattg ccactcgtaa aatattagca aaactaaaaa tagtagataa
      841 tgacaaatta atgctcatta tgcagggtat gcaaatgttc gacctaaata atggcagcct
      901 tctttggggt gtaacacatt cggaagaagt actaaaagaa tctaaaagag gaataccaag
      961 tggctattca ggaagtaaac tagtgaaaag tgcagtgtat ggtgccgttg cagatccttt
     1021 aattgatggc aacgatgtgt atttatttga tatgcaaaac aaaaaaaatc agtatgtaag
     1081 caaatacgat ttacatactg gtaaattaat ttggaaaagt aaagaattaa aaaaattaac
     1141 cattgcacca agcctttata aagtaggcgg taaaattata attcaaattg gtggctgggc
     1201 acaagtacaa ggtattgtag aacagaaatc aaattatgga ggaggatttg gaggttctgg
     1261 tttttcagta tctacttata aacgagtaaa attttacaaa gagtttagcc cttatggtgt
     1321 agaggcttta gatgctacca atggagagtt agtttggcgt agtgaacgtt tttctaaagg
     1381 agttaccaac tcttttgtac ataatggaga gttagtagtt gccagcggca aagagttata
     1441 taacttaaac cccgattctg gaacagagaa atactctgaa agtgtaaaag atgatgggat
     1501 taaagaaaca gaacaaatat ttaaagttgg ggacaatgtg gtggtagttt gttcaaaagg
     1561 aatgagcaac tatgttattg ctgatggcaa aaaaaattgg acagtaaaaa ctaagaaagg
     1621 agacctaagt acagttaata atggcatggc tttttatatt accgaaaaaa atgattttgt
     1681 tgtaattgat ttaactactg gaaaatatac tacttatgat gctcgtaaag attcagactc
     1741 tgaaatatat gaagatggac aatatatact tgtttttgaa aaagataaag taactaaact
     1801 tgctacaaaa caataggtat tagtttatta attggtataa aagctttact ataaaataaa
     1861 cattgtaaat gctaaattaa agtatagaat aaaaagcaaa agagataggt tttataaaag
     1921 gatacatttt aaaattatac tcctatgcag ggcagccaca attattggct gcctctttat
     1981 ttgtaatact aaaaagttta ttttttgtat gtggcagaaa caacaatatc tatttgttca
     2041 gctatttcat cggtaataat tccaaaatct ttaattttta atttaaaaat actatgtaac
     2101 agtatggtat tattgtttac atcaatggtt ccctctgttg taattttttt ggttatacct
     2161 ttaatggtta gattacctac agcattcact ttatacttac cagttgtatt aaaatttata
     2221 ctattaatgt tggtaattgt tccatcaaat tcacttttag aaaacttatc gctatgcatg
     2281 tatttgtctt cattaaaatg tttttgcatt aattcatttt caaataaaaa cccctttatt
     2341 aaagctgcaa attgcacttt tccgttgctg cttattgtac ttattacttg gttattggat
     2401 gcttcaatat cctccacagc agttatagat ataaatttta tcaacccttt agagctgctg
     2461 tatatttttt cagttggttg tacaactttt atactgtctt tttttatagg cacttttaaa
     2521 ttaatagtag aatcttttgt ggaagttggc tcattgctta atgctgtatt tgcaccattt
     2581 gttatcacag cttcgtttaa ttgtatttgc ccaagtatat agcctgttaa aattcctttt
     2641 aaagttatgg ttgcattttc ttgagttgct tgctttgctt ttaaccttcc tgaaaaagat
     2701 ttaccctcta tattggtttg taaaaaaata accatcccag tttcgtcgtt taatattttc
     2761 tccacaacgc cacttacttc aattacttta ttggtatatt ttatattggc ttcagtttca
     2821 tttacttcaa actcttttag taaactagtt actgatagtg caatagcagc ttctttagta
     2881 acatctcttt ttgctataga tggctgataa aatataaaat accatactgt aattaatgaa
     2941 ataatagtgg ctaaaactgt taaccaaaca atactttttt taactttata attaataagt
     3001 ccatatatca ataaacctat aattggtatt aaaccaataa taagccaaac aatttttctt
     3061 gttattgaaa tatcttttct gtttattaaa tgaagaagtg taataattat tacaggaagc
     3121 caagctgtta aaataatagc tgttacccag ggcatttctt ttaaaatatc caacatgaaa
     3181 actaagtttt attaatgatt acaatcgtct ttatgagcat gtttatgaat tctgcaagag
     3241 cgataattta taaaatgtgc tgttacaata aaaataacag caggtattaa taaccaaatt
     3301 tcaaattcgt gccaaatttg tttggcaaat aaaaaaataa tacctgtaat aaaaacaaat
     3361 attggaaaaa aactgtgatg atgtttttta tatccatgat ataatgagta aattccaata
     3421 caacatgcaa taccaatcat actaaactca aaccataaat tatgtataat atttatacca
     3481 aataaaggaa gactggttac aaccaatggt aaaactgcac aatgaattgc acaggcaata
     3541 gaggtagaaa ttcctaaagc gtcccaatta attttaaatg ccatttctgc aaagatataa
     3601 aatgcaataa tgttgcaaaa atattctttt ttagcagttt agcttatcat gtaggcttac
     3661 ctttgccaca tgatgaaaaa gaaaaaatgg atattttatg ttactttttt tgtaggctta
     3721 atattggttt tttggcttgc attgtatttg tttacagata cttttaatca aagtagatta
     3781 gtaaataggt ctgttgttaa gccatttact ttttataaag aagatggtag tctgtttacc
     3841 aaggcagata tggcaggtaa ggtttgtgtg gtggaattct tttttacaac ctgtccaaat
     3901 atttgccctg aaatgaatac caatatgaaa aaagtgtatg aagcatttaa agatgaacca
     3961 aattttttaa ttctttctca cacttgcgac cctaaaagag attcggtgcc tgtattaaaa
     4021 aaatatgccg attctatgca agttaataca caagtttggg catttttaac tggcagtaaa
     4081 gatagcttat ataatatggc tagagaaagc tatggtattg atgaccctaa aagtgtggta
     4141 aataatattg aggacgattt tttacactct caattttttg cattagtaga taaaaatgga
     4201 aatgtgagag gccaggttta tgatggattg aaacaagacg agataaaaca actaaaaaaa
     4261 gatattaagg aattgctgaa tgataaagga aaccgaaggg gtttttctaa taatttattt
     4321 aattaaacta aactttttac catatagaat aaatatgtag catgcaaact gaatttttat
     4381 cgatactaaa aaaaaacaac ctaagtgtaa ctgaaagtag aatgaaaatt cttgaattat
     4441 ttgcacaagc taatggggca ttggcacata acgatattga aaaaaaaagt aaacttcaat
     4501 ttgatagggt aaccatatac agaaccttgc aaacttttgt agaaaagggc atcattcata
     4561 ctattcctac agcagataac agtgtattat atgctttgtg taaagatgag tgtacacaag
     4621 ggcatcataa cgacaatcat gtacatttta tttgcgatag ttgcaatgta acttattgtt
     4681 tagaacattc aaactcacct tctgtaattt tacccaaagg gtttaaacaa aatagagtgg
     4741 atgtggtagt tagtggaata tgtttaaaat gtaatgctta aattatgatt ttagttacag
     4801 gtgcaacagg gcttcttggc aacgagttaa tacaacaact tattacccaa ggtaaaaaaa
     4861 taagagcttt attttatcat accaacccaa cgttgctaca tgaaaatata gaatggaaac
     4921 aagtagatat attggatatt gttgacctgg aagaagcaat gacaggtatt acacaagtgt
     4981 accattgtgc tgcacttgtt tcttttaaca aggcacaaaa aaattattta caccaaataa
     5041 atattgaggg aactaaaaat attgtaaatg cttgcttaca agaaaaagta gaaaaattat
     5101 tgtatgtaag cagcgtagca gctttaggca gaataagaga agataagccg attgatgaaa
     5161 cgatgaattg gacagaagaa acaagcaata gtgagtatgg taaaagcaaa taccttgcag
     5221 aaatggaagt atggcgtggt attggcgaag gtttaaatgc tgtaattata aacccaacaa
     5281 ttattttagg ggcaagcaat tggagcaaag gctcaacgga aatttttaaa acagcttaga
     5341 acgaatttcc ttggtttaca aatggtacaa caggttttgt agatgtaaaa gacgttgcta
     5401 aagcaatgca acttttaatg aatagtaata ttaatgcaga aagatttatt ttaagttcgg
     5461 aaactatgac ctataaagat atttttagta ctatagctaa agcatttaat aaaaaaccgc
     5521 catataaaaa agtaactcct tttttagctg ccttagtttg gagatgggag gctataaaag
     5581 gcaagctaac aggcaaacat ccattgttaa caaaagaaac tgctaaaaca gctcaaacaa
     5641 aagctagttt taataatagt aagttattaa attttttgcc agagtttaag tacaataaaa
     5701 tagacgaaac tataacaaga atatgttgtg aattaaaagc aatgtataaa ctataaattt
     5761 atactaagcc aaattgtttg caatggtgta atacatgttt gtaatgtaat aaaacccatt
     5821 cgtcaaaatt taaactacca aatacaggat gaatagtttg ggcattttta tgtaaatgaa
     5881 agtattgaac aaattttgtt atttcctgtg ttagtaaatt aattgcaatt tcaatagatg
     5941 catttattaa aggcttaggc tctacaggta ttaactctac aggagcttgg gtgttttctt
     6001 taaactcttt atcactcaaa ataaatgctt tgtatttagg aagtaaatca acaggtgtaa
     6061 ttatattttg ctgtaaaggg ttggtagata ttgtaaaaaa ttcagtaaca tgctctacca
     6121 tttgttgcaa attcatttta ccccataaag gtttactgtt ttcattagca gtttttaata
     6181 atgcaggaaa tgattgtaca ataaattcta ttttatttga gtgcatacat ttttttaaaa
     6241 tgagttacat atttatgtaa aacattaatt ggtttaaatt ttcttttaat tcattattat
     6301 actgttcttc tccaaagtaa taacgtatag tgtaatcata tctttgaagg gttgcaacaa
     6361 tatcgctaat ttctatagta ttaactttta tggtaacaat aaataagcct gtatcttgct
     6421 ctacataagt atttagctgt gttatgtagg agtcattggt ttctattaat ctgctaattt
     6481 caccaaaaga gaaatggcgt ttttcagttt ctaaaattat cattccacca ggttcgttac
     6541 aacttaaata aagagctgta gcatttacta attcatttaa tggaataata ccttgtaact
     6601 ctatttgttc attaacaaca gcaattgctg aaagctggta ctgtgctgct aatttaattg
     6661 cacttaaaaa atattcctct gtttttacaa aagcagtatt aaaataattt tgaagagttg
     6721 ctataatatt gttttcatca gcatctaata aatcatcttt actaattata ccaacaaatt
     6781 tatcttctga tattattggt aaatgttgaa tatcaaaatc gtctaaaatt cttaaagcaa
     6841 caattacttt atcgtttagt tgtattatag gaaaattggt ttgtatgatt tgctttgcta
     6901 acatttttta tattctaaaa caattataca acaaaagatg ctgcatttat ttgtttttac
     6961 ttaaaaatgt atctaaaatt ttattaaatt ccaagggcac ttccatcata ggggcatgac
     7021 cacatttatc aataaaatgt aattcgctat tgggtattag cttattaaac tcttgcccta
     7081 caaatggggg tgtaatagta tcgttattac cccataccaa taacgttggt tgttttattt
     7141 ggtttaattc ttctcctaaa ttatttctaa tggcactttt tgctaaagca ataattttaa
     7201 tcacttttaa tcggttattg gtaatttcaa aaacctcatt taccaattct tctgtagcca
     7261 ttgcaggatc ataaaaagta agttctgttt tttttctcat gtactccctg tctccacgtt
     7321 taggataact atcgcccatg ccgttttcaa ataaaccact actaccagta agggttaaag
     7381 tttttatttt ttctggatgt tttaatacat gcaccaaagc aacatggccg cctaaagagt
     7441 tacccaataa atgaatatta ttatatcctc tggcctcaat aaatttatga acaaatttct
     7501 gtaaaccacc aactgtagta tgaaagatat ccaactcaaa tagtggtaac attggcacaa
     7561 taaccttatg cgttttttta aaatgctcca ccaaatctgc aaagttactt agtgcaccaa
     7621 ataagccatg aagtaacatt aatggctctc catctccttc ttccaggtac ttaaatttat
     7681 cttgttggtg tatagtataa ttcatattgt aattggttgc tatttggggc tatgagaatt
     7741 ttacaaatta aaggaaaaat gtagaatagt ttctttactt aattattaaa tttaaatatt
     7801 aaaatcaccc tacttgctaa gttatagttt ttgctcaact aaaaataagc ataattatat
     7861 aaaaggataa ttatttttta ctaattaagc attgcttgct atagaagaaa aaaatatttc
     7921 aagttgttca aaaatatttt taaacattgg tgtaatagag cctattacct gaattgcctt
     7981 tggtgcaata ggctgtataa aaaaataagt tttagattgc tgcactgtat tggcagaaag
     8041 gaggttcatt tctatcaaat aaaatagaag aatgcttaaa actgaaaagt gcaaaagtat
     8101 gtataaagct attcctgcaa ttttatttaa ccagcccatt aatgcaaact caaccgtttt
     8161 ttcaataata gcagaaccaa ctctaaccaa tattattgtt gccactacta ccaacataaa
     8221 tgataaaaat ggcagccatt taattggtat ttttatattt gattgttgta aataatttgc
     8281 agtaactgca ctaagcttta aggcaacaag caaccctata aaaatactta tagttgaaaa
     8341 tatggctact ataaatccct ttgtatagcc tttaaaaatt gccaatatta atagtaccaa
     8401 tacaataata tctattagca tactgaaaaa ataagtggct taaaataaca ttaaaccgat
     8461 agcaattttt ttactatagt acttataacc ttaccatctg ctttgcctgc aagttgcttg
     8521 gttgcaatac ccattacttt gcccatatct gctgcactgg ttgcattaga attggtaata
     8581 atagttgtaa taacttgttc taattcggct tcggttaatt gctttggtaa aaatttttca
     8641 attacttcaa tttcttcttt ttctttctgg gcaaggtcgg cacggttttg ttgtgtaaaa
     8701 atttcaaaac tatcttttct ttgctttact aatttttgta aaagttttaa ttctgtatcc
     8761 tcgtttattt gtccatttgc accaggttct gttttagctt ttataatttc tgctttaata
     8821 gcccttaagc cacgtaaaac ggcttcgttt ttagctttca tggcctcttt catttcgccc
     8881 attatttgta tctctaagct cataattaat tataatttat tttcttttaa ttgttggtct
     8941 ctaaatttat cataaaaact tttagcaaag ttttttaagt cgtttaccat ttcttggtta
     9001 ttggtggctc tgccataagt atctgccata cccataaatg tttgatagta aaaatccgac
     9061 atttcatcca ccatcatatc tttagtccaa aggtcaattc ttaaagcagt tttttcggct
     9121 ccatcccata gagctagtag tactgctttg gcaaactgtt tattctctgc actactattg
     9181 cttgctttcc aacttatttg ttcaggtatc ttattttcat ctaaaataac atctaccgtt
     9241 atagttgatt tttccataaa agtgtattta aaaaataatg gcaaatgtaa aactattatg
     9301 gcattgcata atgttctata atggttattg tttcattaat acttttatcc caattaaatt
     9361 cttgtgcctt tttaattcca ttttcaatta aattattcct caaaccttca tctttataaa
     9421 gcatcatcat ttgctcggca atattctttt cgttattggt atcaaaaaat aaagctacat
     9481 tgttaaacag ctcagctaaa cagcctgtat tgctacaagc aataggtacc ccagcttgca
     9541 ttgcctgtaa agcattaaat gcaatgctag tgtgctcata ggtataaata aaagcgtagg
     9601 ctgccgatgt tattttttgt agtaactctt ctgaggcatt ttctattaac aaaacatcct
     9661 ctttaaactt ataggttttt aatttttctt ttaaaatggt tgtgttacta aaagcatctg
     9721 caattaaaac taacttcata ctgctctgta atcttttttt aaataaagta aaagctttaa
     9781 gtaactgaac taaataatgc tgggtattaa aactaccaaa gtataaaaaa tattcttcgt
     9841 cgccggtaag tgtttgttgt atcttttctt tttcaacata agtaatagga ttatatgttg
     9901 aagaaataac cggggaaata aattctactt tattattaat tctataattt tcaaaaattg
     9961 ctttttgggt aaataaacta aaagtaattg tagcagaagc tttttgtatc catttttttt
    10021 gatataagga atacattatc cttgctgtgt tacttaagtt atttgtttgg tacagaaaag
    10081 ataaatgagc aatagtaata atttgagggg tttttaaacc tacaatacac ctactatcta
    10141 aatgccaaac aatagagggt tttacttttt ttatgaaagt agccaacttt atattgtacc
    10201 aataggaaag ggcaatactg tttgtagatt ttaaagaaat tatttttaca atacaatttt
    10261 ctgcttcagt tatttcaaaa ttgttttcat cttcggtaat aatataaaac tgataatgtt
    10321 tatacctgct tactaattta ttaatcatta cctgcatata atttctgtat atacaggtat
    10381 tattaaacgg aagcattttt gtattgataa caatacgcat aagggcaaat gtaaagtttg
    10441 ttagtgtaga atgtaattta aattactaat ttgctaaagc aggaatttct tttaagcttt
    10501 aattgtaaac tgaagcctac atgcttattt ttacaactta cttatggctt attttaattt
    10561 aaccgacact ctaaattttt tatcaaaact tacatttcgc agagcatgga atgcctataa
    10621 ggtatggtta agttttcaaa taagtaaata ttcaggaaag ccaattcaat ggggctaccc
    10681 aatttcaatt tcatttgagc ctacaacaag ttgtaattta agatgccctg aatgtccaag
    10741 tggtttaaga gaatttacaa gaccaacagg aatgcttcaa aatagttttt ttaaaaaaac
    10801 cattgatgaa attcataaag agctgttgta tttaattttt tattttcaag gcgaacccta
    10861 tttaaaccct aattttttag agatggttaa atatgcccac gataaaggaa tttatacagc
    10921 cacaagtaca aatgctcatt atttaaccga agaaaaagct aaaaaaacag tagaaagcgg
    10981 gttagatagg ttaataattt ctattgatgg cactacacaa gatgtttatc aacaatacag
    11041 aaggggcgga aaattagaaa aagtattaca aggtgcacaa aatattgtaa aatggaaaaa
    11101 agagttgaat agtaaaactc cgtttatctt ttttcaattt ttagtggtta aacccaacga
    11161 acaccaaata gaagaagtta aaaaattagg aaaagaaatt ggtgttgatc aggttaggtt
    11221 taaaacagca caagtgtatg attatgaaaa cgatcccaat aatttaatac caactattga
    11281 aaaatacagt aggtataaaa aagataaaac aggcagttta aaagtaaaaa gtggtatgca
    11341 caattactgc tggcggcttt ggcatgcaaa tgtaattaca tgggatggtt tagtggtgcc
    11401 ctgctgtttt gataaagatg ctatgcaccg gttgggcaat ttacaaacgc aaaattttaa
    11461 agatatttgg cataacaaca attataaaca ttttagagct gagttgatga aaagcagaaa
    11521 aaatatagat atatgtgcca attgcagtga agggttaaag gtttgggaag actaattaat
    11581 ttactttaac aatgtaataa cccactttaa aataaaataa ttttttatta aaatagttta
    11641 gctaattttg tacccttatt tatcattttt tttacttaaa aaactaatta tgaaaaaaag
    11701 tttactctta ggcttattgt ttttaataag cacaataggt tggtctcaaa ctatttcagt
    11761 tgcacaagct gacaaagcag gaagttctag taatgttata tctggtgcag gagcttatca
    11821 tgcattagaa gctatttatt tagatgctga aattggtgct tcaaatttta ttggtgtggg
    11881 caatgaaata caaaaaatta gatttgcagc caatacaaat agtacaggta cttttcctgg
    11941 ctctattgct ggctatagta tttatatgaa aaatgtggct gcaggtacta aaacatttac
    12001 agcaggttca tattcattat ctggctatac tttggtttat actggtgctt taagtgcaac
    12061 ttctgctggg gcaccatatt catttacttt tgatgctgaa gtaacactaa caacaccttt
    12121 tgcacgtact gctggcaata acttacaagt tttaatatta agaaatagtg gaattgccgc
    12181 aaccgggtat gcttgggatt gttctaatgg caactctgta gatgggggta ctgcaaatac
    12241 ttctagaaga tataatggtg caacacaagc ggttgaaaac agtacatctt tatctgtttc
    12301 tgcatttaga ccagctatac aattaattcg tcctgtttct aacgatgcta ctgttactgt
    12361 agatggtgct actcttccaa ctgcttcttg ctttagcaca ccacaaactg ttattgcata
    12421 tttaaaaaat tcaggtacat ctacaattgc tattggtgga gcttctgtaa aaatagatgt
    12481 tactggtgct aatacataca caactactgt aaacaacact ggtgctttag ctgcaaatgc
    12541 ctcagaaatg ataatttttt ctggtattaa cttaaacaat gctggtgtta acaattttaa
    12601 aatttatagt acaatggcag gcgatggaaa tgcaactaat gatactgctg catttacaat
    12661 taccacaacc gccacataca atacataccc agttaacact agtgcagaaa caaacaattt
    12721 ggtttttcaa tatttaaaaa cactaaatgt tagtaatcac tggacattaa actctgcaat
    12781 aactgctagt gcaacaggag cttttaaaaa taccgattta accgactcta tcttccctaa
    12841 aactggtact agattttatg ttcaagataa ctactctggt gcaaatagta cagggcacag
    12901 tagtgtttta tatgcaggct gcttcaactt ctcttctcct gcaaacatct ccttctggat
    12961 gactcaagat aatagcctta atacatcttt agatagtatt tatgttgttg tatctacaga
    13021 caaagctgct acatggacaa gattacaagg ttttggtagg cttagtgcta gtgcattatt
    13081 accaacatgg gttcaacaaa ctgtaagctt aaattcttat gctggccaaa ctatacatat
    13141 tggtttagaa ggtgtaagca aatggggtaa tgttataggt gtagatgata tttctgttac
    13201 tgctaatgca cctgtacctg taacatttgg cgaatttgtt ggtgtaagag aaaataacag
    13261 taatcaatta aaatggaata cttatacgga aactaataac caaggatttg aattacaacg
    13321 tagtgttaat ggtacagagt ttacaactat tgctttcgtt ccaactaaag caattaatgg
    13381 taatagtaat tctgaattaa attatacttt ccaagataaa gctagcagtg ctactactta
    13441 ctatcaatta aaacaattag ataaagatgg caaatatgct tattctaaag tagtttctat
    13501 taaaggcgaa aaaccaacca gatttgaatt agctaatttg tatcctaacc cagctaaaga
    13561 aactataaaa gttactgttg ctgcacctaa agctgctgta gtaactttaa ctgttacaga
    13621 tatgactggt aaagtggtta tgaaacaaaa taatcaatta actatgggtg ataataatat
    13681 agctttaaat gttgctgctt taaatgcagg aaattatatt gttagattat cttgtgctga
    13741 tggttgtgaa tactctattc aaagatttgt aaaacaataa aagttttata aagaatatat
    13801 taaagatgct gcttgaaaac aagcagcatc tttttttgtt attattttta aatgttctta
    13861 aaatttatat tttactttaa ataagatatt attttataat aaatgtaagc taacatagtt
    13921 gctctctaca gccttaccat aaaatatgct tgcttgtttg ttgaacatta atggatcatc
    13981 tgttgtaaaa aaattagtgg agccctcttt tttacaaata gcatctaatt gttgatgcct
    14041 atttaagtac tggcttaaac tagtagcaac aatttcacct tgggtaacaa tagccatatc
    14101 ggctggggca tattctctta ttttatttat tagcaaaggg taatgtgtac aagctaataa
    14161 tatggagtca atatcggcag attgttctgt taactggtta atatattttt ttacaaaata
    14221 atcagcccct ttactattgt attcattatt ttcaattaga ggaacccaca ttgggcaagc
    14281 ttgctggtaa acttttatag aagggaaaaa tttttcaatt tctaaaatat aagatttaga
    14341 ttgtatagtg cctgtagtac ctaatatacc aatattatta gtgttggtat agttacctat
    14401 tacttctgcc gtaggcctaa taacgcctaa tactcttttt aaattattgc ttaagctggg
    14461 taaattgttt tgttgtatat ttcttaaagc ttttgcagaa gctgtattac aagctataac
    14521 aactaaatga cagccttggc taaaaaacca ttgtacagcc tgccaagtat attgataaac
    14581 tgtttcaaaa ctacgagaac cataaggtgc tctggcatta tcgcctaagt atataaaact
    14641 atattcgggc aatttttgct ctatatcttt taaaattgtt aaaccaccgt aaccactatc
    14701 aaatattcct attgctcctt tatgcatatt tttatttagt gcttaaagtt aattgaagaa
    14761 agttaaaatc taaaattctg catttttatt aatacttaca gaagaaaaag ggtacaaaaa
    14821 agctaccttt ttaggaggta gctttagaaa atatgttaat ctacaaaatg atttaattct
    14881 tctttggagt agtggttggt tttgttgcag atgctccacc aataccaaat gatttatatt
    14941 gctcttcaat ttcttttgga actggcactt ttaatttgtg taatacacgt agcattaatt
    15001 cctctccttt ttctatatgt acaaaaacat ccttcttgaa aatatgagta tatttttgtt
    15061 caataatgat agcttgtaat gcaccataaa ttttttctaa atatggcctt aataactctt
    15121 gttgtttaat ttgtaatctt tgttgttggt attgctgcca attttgtaat ttattaaaat
    15181 gtaaggcaat ttcttttttc aaagttaact ttaaagagtt gctcatttta gaagagtctg
    15241 ctttaaaagt actatctttt gtttggtaca aatacaattc atcttcatac tcattttgta
    15301 aagagtcttc tacaaattga tgtaataaag tatctacttt agaaatgcca ggcataaatt
    15361 gtaatactgc ttcttcatca aacatgccta ttttaatttg ttgggcattt gattttgttg
    15421 ttgctgcaag gctaataact aaagcagcac aagccataaa aactttcttc atcctttttg
    15481 tttatttttt atttaataaa tagggtttat ttaattccaa gttctttaag tatgtcttcg
    15541 cttttatcaa ttttagggtc ggcaaatata acagtaattc cctcactttt gtctaatata
    15601 aaatcgtagc cacggttaac ggcaattttt tgtactgctg tataaactct gtcttgtatt
    15661 ggttttacta atttttgtct ttctttaaac aaatctcctt catatccaaa gcgttttttt
    15721 tgtaattctc ttacttcttt ttctttgtta aaaatttcat cttctctttt tttctttaaa
    15781 tcatcgctaa gcataaactg ttctgcttca taatctttat acattttatc taactctgct
    15841 tgtttgttgt caatttcttt ttgccattgc tctgcaatag attgtaattt tctatcagca
    15901 tttttatatt ctggtatttt atctaaaata tattttgtat caataattgc ataacgctgt
    15961 gcattaataa aaatggtggt tgctaaagtg caaaccaata atagaataat ttttttcatg
    16021 agtttatttt tttacttgaa aaattaattt ttattcaggc tcaaagccta acatgaatgt
    16081 aaacttagcg gcatctttta atttgccatt tggtgttatt ctatccaatc ctatgccata
    16141 atcaaaacca agcaagccaa acattggtaa ataaaaccgc atacctatac ccacagaacg
    16201 tcgaagttga aatggattat aatctttcat actaaaccag ccatttgcag cttcaaaaaa
    16261 gcctaatgca taaatagtgc tacttgggtt taaacttaaa gggtatctta gctctaacac
    16321 atatttgtta aaaatggtaa agtattcaga agctccttgt tgatctggat ttacttttgg
    16381 attagaagtt tggtacacag gatatcctct ttgtgcaata atatcataac caagtaatgc
    16441 aaattgatta gatagaccag catcgcctaa ttgaaaacgt tcaaatggag aaacctttaa
    16501 tttattattg tatttaccta taaaaccaaa tttggcagcg gcttttaaaa ctaattgttt
    16561 gtttttttct ggtcctacag ctttggttaa tggtacatac cattctgaat tgaaacgcca
    16621 tttatgatac tcaacccatt ggtaagggtt aacagaggta acaattgttt tgtcaaataa
    16681 agaataagga ggtgttaatt gtaagcttgc taaaaagtta gaaccctgtc ttggaaataa
    16741 aggattatcc acagaggaac gttgtaaagc aaactttaaa cttaaattat tagaaaagcc
    16801 attattaaag cctggtaaat ttacctgatc tatataataa tttttaagtt tataccttga
    16861 aaaacttaac ccaatgctta atgaaaaata atcatcgggc cattttaatt gttttgctaa
    16921 acttaccgat gcacccgatg ttttgatata cgatttattg gctgcattgc ttgtataggt
    16981 gcctgttaaa gggtcgtagg cattagaaaa tttagtatca aaaacggaaa tggtaaaagc
    17041 atttcttttt ttgccaccca gccaaggctc ggtaaatgaa aaattataag atctaaaagc
    17101 tctaccattg ctttgaacac gaaggcttag tttttgacca tcgcccatcg gtaatggatc
    17161 ccaagcttgc tttttaaaaa tatttttaat agagaagttg ttaaaactta cacctactgt
    17221 accagttaat ccaattcctc ctccccagcc tgcactaagt tctaattggt cgctgctttt
    17281 ttcttctaga gaataatcaa tatctactgt tccatcttct ggattaggac gaggattaat
    17341 tcctattttt tcttgattaa agaaatttaa ttgagctatt tctctttgag aacgtattaa
    17401 atcgcttcgt ctaaattttt ctccaggaat ggttcttatt tctcttctta ttacgtgttc
    17461 tttggttctg ttatttcctg taatatttac atctttaatg gtagcttctg gcccttcggt
    17521 aactctaatt aaataatcta tggtatcgtt ataaacagca gtttctatag gttcagttct
    17581 aaagaaaagg taaccatcat ccatatataa cccacttaca tcaccccctt ctggcgatag
    17641 ttgaatgcct aacccttttt ttaatgtttc taaattataa acatctcctt ttttaattcc
    17701 taaaatagaa gttaatacag agtcggagta tttggtgttt cctctccaag ctatattgcc
    17761 aaaataatat tttttacctt cgtttacttt tatactaatg tttaaatcgc cttttgcatt
    17821 gggtaatatt tttatatctt ctttattgat tacagcatct ctatagccat tagtattgta
    17881 gtattcaagc agtttttcta tatcgccatt aaatttttta tcattaaatt ttgccgatga
    17941 caaaaattta aatctaatat atgggtctaa taattttttt gttttactat aggttaaaaa
    18001 accattttca tttaaatagt ctttaaatgt atattttgtt attcctgaaa atgcactttt
    18061 atcatcagat ttttttattg taaaacgact tttttcttta gtatctttta attgtttttt
    18121 tagttttaaa gggtctaaag taacattggc aaagtcaata ttatttactt ttactttgtt
    18181 gcccttatta atatgaaata ctaattctat agcattattt attatagaat ctttcttttc
    18241 atcaatagtt acagttacat ttctataaga tttttctgca taaaatttaa taattgcatc
    18301 ttctgcattt ttcttcatat tttctgtaac aatacgattg ggaactaagc ctgttttgcc
    18361 aattaaatct tctttatcgc ttttcttaat tcctacaaaa gtaaaacgtg caagtcttgg
    18421 tcgttccgta acatgaattt caagccaaat gcttttatct actagtttag atacataaat
    18481 tgctacatcg ctaaaataat tttgagccca aagtttttga attgcttttg caaaattatc
    18541 tccacctggt attattattt catcgccaat atttaagcct gaaatagata ctaataatgt
    18601 ttcatcaaag taattattac ctactactgt aataccagct attttatatt tctgtggttt
    18661 tttttgattg aaaaggttaa ttaattctgc atctaccgat gtaatagtag tatccttatt
    18721 attttgttgt gccgataatt gagctgtact aaaagcagtt aaaaaaagca gaagtaaata
    18781 ttttgtagat aagcgtttgg acatattttt gtcttaagtt ataagtcttc taaagttgca
    18841 attacaggca atttcagcaa gggatttaaa agcaaattaa agggatttct taaaagggtt
    18901 tgttaaataa aaagttatat taatttttag ggcagtatta tttattttaa acatagtaac
    18961 agtactatta attgatttaa ggatggctag tagaatttaa aactatatta tcatgaattt
    19021 gcttgcttgt ttttccaaat cttctctctc tgccttgata ttctatgatg gcttcatata
    19081 ggttgttttt tctaaaatcg ggccaacaag tatttgtaaa atatagttca gagtaagcca
    19141 actggtaaag taaaaagtta ctaattctaa attcgccact agttcttatc attaattctg
    19201 ggtctggaaa attagcagtt gttaaatatt tttcaaatac agtattgtta atactattag
    19261 ggttaagatt gcccatttct acatcttttg ctattgcttg tacagcatta ataagctccc
    19321 atcttgagct gtaacttagg gccattatta aatttaaacc tgtattattt tttagtaaat
    19381 ctatagcttc attcagttca tttcttgcca attctggcag catacttgta ttgccaatta
    19441 catgtagctt aatattgttt ttattaagtt ctggcacttc ttttctaata gtatctacca
    19501 ataaagtcat taatccatct acttcttgtt gtggcctatc ccaattttcg gtactaaaag
    19561 catataaagt aaggtattga atacccaatt cggcacaacc ctctacaata tttcgtacac
    19621 tttcaactcc atgaaagtgt ccaaacaaac ggtcttctcc tttttcttca gcccaacgtc
    19681 cattaccatc cataataata gcaatatgtt ttggaaggcg ttgtttgttt attttatcaa
    19741 ctaaaggctg cataaattgc ttttaaggct gcgaaattag taaaatacat ataattatat
    19801 taaagtaaag cagcaaaaag tataataaaa gtatcgttta tagtttagtt tattaatatt
    19861 ttttaaaaaa tatctttaaa taatttactt tagcccctgt tttaaccttt taaacaaatt
    19921 atatccccta cagtgagaca gtttttaata attgcctttt tagtattaac cactaatatt
    19981 caagctcagt ttacagtaac ccccaacaac aatgcaatgc agctagctca aaaaattgtt
    20041 ggacaagggg tgcaaattgt aaatgcatgg tttaaaggct cttctacctc tgctggtttt
    20101 tttacagata ataccggaac tttaggaatt agtagtggtg tattattaac ttctggtaag
    20161 gcagccaatg ttgctgcagc agcatcctca caaaacgatg caacttttgg tgccggagat
    20221 gttgacttaa ctaatttagt tggaactggt actggtaatg acgcttgtgt tttagaattt
    20281 gaatttatac cagttggcga ttctattgtg gtaaaatatg tatttgcatc agaagaatat
    20341 cctgaatatg cctgctctga ctttaatgat gtttttggtt ttttattaaa aagcccatta
    20401 cctatatttc ctcctaatgt gtttaaaaat attgcgttag tgccgggtac aaccataccc
    20461 gtgaaaataa acacaattaa tcctggagca ggtgttaatg gccaaatagg aaactgtacc
    20521 tctcaaggta ctggctctcc ttttactcag tattatagaa ataatttatc caactcttat
    20581 ataatttatg atggcatgac tacaatattg gttgctaaag caaaagtagt accctgccag
    20641 ataaacagaa ttaagattgg tgtgcaagat atggttgatg gcgtttttga ctctggtgtt
    20701 tttatagaag cgaatagttt tagatctgat actacatcat taattaaaac tgggggatat
    20761 acagatgctc aaggaaataa aaatgtagct gttgagggtt gtagtaaagt taaagtaaaa
    20821 ataactttag aaaaacctgc aggaccaagc ggtaaaactt tgcaattaaa ttattcaggt
    20881 tctgcaacac gtggtgtaga ttttgatgct gcccttgcac caacaagtgc agtatttgct
    20941 gcagggcaat cggattttga attagaaatt aaacctattg tagatgcagt tgttgagcca
    21001 ttagattctg taattatttc tgcaggaatt gcttgtgcta cctccactgc aaaaattaca
    21061 gtttatatta aagattctat tacattcaga aattcaaaag atacttttac ctgttcttct
    21121 ttccctacag tacttacagc tagctacgac tcttccaata ctaatgtgta ctcttggaat
    21181 gtaaccccag cagtaactac tcaaacttta agtgttacaa aacctggtgt ttatatagct
    21241 acgaatacat ttttaaatag ttgttataat gtagatactt ttaaagtagt taaaggagat
    21301 cctgatttat ttttaggaaa tgatacaaca ttttgttcaa aagatagcat aatattaacg
    21361 ccaattaaaa gcccttacgg aggtagcttt acttggcaga acaatgcaac agatactttt
    21421 caggttgtaa aaaacacagg aacatattgg acaaaatata caacccctaa tggttgttat
    21481 gtttatgata ctgttgaaat aacaaaagtg caaatgcctt atccacagtt tggaaatgat
    21541 actgcttttt gtgccaactc tagtgttact ttaactgcaa ctaattatac cggggctact
    21601 tatttatgga acacaggagc aaccaccaac acaattaatg taaatagttc aggcacctat
    21661 tctattataa gtacaattgg gctttgcgaa tcaaaagata cgattaatgt tatcaaatct
    21721 gatttaccaa taaaaaatct tggaaatgat actacttatt gtggaaataa tggtattgtt
    21781 ttaaatgcaa cctacccagg tgctacaacc tatttatgga atacaggtgc aacaacagct
    21841 accattaata ttaccagtac tggtacttat tctgtaacaa ataatttgaa tggttgtatt
    21901 gcaaaagaca caataaaaat tactattaat cctataccat atccacaaat gctaataagt
    21961 gatacagctt tatgccccta tgaaaatata aacttaaatg cattttaccc aggggctact
    22021 tatttatgga atacaggaga aacaaccaat attattaatg caaaaactgg cggactttat
    22081 attgttacta acactttaaa tggttgcatt gcaaaagata cagtaaatat taaaaacaat
    22141 aaagctgcta ttgcttatgc aggtaacgat gtattaatgt atgccggaag ctctgtaaat
    22201 ctaactgctt ctcaaaatgc aaataataaa acctatcaat ggactccatt aaataattta
    22261 tcaagcccta attattatac caccaatgca agtcctaaag atactacaga tgtgttttat
    22321 tatttaaaag ttacttctgt agataattgt gtagcgtatg atactatttt agttaaaata
    22381 atgaatgcta agtttgatat tccaaatgca ttctcaccaa atggcgatgg cataaatgac
    22441 ctatgggaag ttcctttctt atattctttt ccaacttcta gaatgcaaat ttttaataga
    22501 aatggacaaa taatttatac aagtgggggt actaattaca agccatggaa tggaatgtat
    22561 aatggcaaac cagcaccaat tggagtttat tattatgtaa tagaggtagg caatggtttt
    22621 cctagaaaaa cgggttgggt agcaattgtt agataattaa ttaccaagaa aattttaaat
    22681 tcttagttgg aattagggtt caaaatagtc atctatttaa ttacattttt ttaaatatta
    22741 ttatgcttgt ttgctttttg caagtgtaaa agtttagcat attttaatag ggtttgatag
    22801 gcaatggttt tagctaatat aaatccatct ttaccatcta aaaaaccaag cttaataaaa
    22861 taaccactta taaatgcaac acatgggttt acaattaaat taattacaga agctctttta
    22921 ccttttttat acattgcatt agcctgtata gttgtaaact tatttgcttg tgtaataact
    22981 tcttctaaac tattgtaact ataatgtaaa atatctcctt taatataaat acaacttgat
    23041 tgcttatcta tttcaacaaa atcgtgtggg tttgtgccag cccattttgc attatgctta
    23101 ttaaataacc taattttttt atcaggatac catgtgccat gttttatcca tttgccacaa
    23161 aaattggtac acctattcat tttataacta tctgcaacaa cctttttttg tttaattgct
    23221 aaaatagatt gttgaagagt ttcgtctaat gcttcatcgg catctaaact taaaacgtag
    23281 tcaaaataag ttagtgctac tgcaaaattt ttttgctcta tataacctaa aaaaggctgc
    23341 tcaataaatt taatagggta tttactgcaa atattttttg tattatctgt tgaaagggag
    23401 tcaaccacaa taatctcatc agcaatttgt aataagcttt caatacatcg gcttatattt
    23461 ttttcttcgt taaaagttat tattgcagcc gaaatttttt gcatgagaat attttggaaa
    23521 agttatgtat tatttttttt ctgcaattac aatattattt aaatataaat cattgcgttt
    23581 gggcattagt ttgtgcaata ataaaaatag cccattacac aaaaacatta aaggtgacct
    23641 gatattggat gttataaact ttagtttgcc aatgataaaa atagattgca gaaaatgctc
    23701 acttatataa accattttca tttggtgtag tgtagtggca aaatctccag atttgtctaa
    23761 aacaattatt ttaaaaccat ttttttctaa taaatgcttt aatgcaaact gcgtatatct
    23821 ggcataatca taaggaactt catgttcgtt ccaaacaaaa ggacaggtaa ttaaaatttt
    23881 accaccaggc tttaaaactc tattcaaatc aggtaaaatt tcttctaaat taaaaacatg
    23941 ttcaaaaacc tcgctacaca gtatagagtc aaattcatta tctttaaaag gaatattttt
    24001 gccatcataa aatacatcta tttgttcgtt gatgtgagaa tggcctgtgt tttcatagtc
    24061 aactcctgta taagagctaa catggttaaa taaactttta tatggttttg agccacaacc
    24121 aaaatcaagc aaattacctt gtaagtgatt tttatattca ataattttat gcagcaaaag
    24181 ctttcttata aaataatacg cactagttaa aggtggatta aactgcttgg taactttagt
    24241 ttgcatataa tttatttggt cattattctg cctgtgtaat caattctttg tagtaaaggg
    24301 taaaaatttt gggttgcaaa atattcatcc actgcttttt tgcaaccttg ccagt
//
LOCUS       DAOQPK010000006        24153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_990_length_24153_cov_4.727903, whole genome shotgun sequence.
ACCESSION   DAOQPK010000006 DAOQPK010000000
VERSION     DAOQPK010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 24153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24153
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_990_length_24153_cov_4.727903"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(433..2076)
                     /locus_tag="PLU36_00655"
     CDS             complement(433..2076)
                     /locus_tag="PLU36_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HMZ45290.1"
                     /translation="MKLFSTLLLSILTTLSFAQKTTSTATPNTSKPKLVIGIMIDHMR
                     WDYLYKYQNRYTNDGFKKLLKEGFTCENTFIPYTPTATACGHASAYTGTVPAIHGIAS
                     NNWWDMKQQQYVYCTDDSTVKGVGSNSKKGLMSPRNMVANTITDELRLASNFKSKVIG
                     IALKDRGSILPAGHSANAAYWYDETKGIFISSTYYMNDLPKWVKDFNAQNWVDKYYAE
                     GWNTLYPIETYTQSEGDDNNYERTNWIEVDKPVFPYKFSQHISKDYKKFSYMPQSNTY
                     TLNMARAALKAENMGKNGVTDFLAISLSAPDYMGHSFGPASVEAEDMYLRLDKDLAAF
                     IQYLDTYIGKGQYTLFLSADHGGAHIAGYLQKHKIPGGSLGLKKIVSDLNKTIAQQFG
                     ISKAIAYQTYYNISLNHAQIDSAKINLQTIKDFIINSIEKEDGVYRVIDKQKLETSGL
                     PFKIKESLINGYYPNRSGDLQVIPLPQWMRDTEKGTDHSAWNPYDAHIPLVWYGYGIK
                     QGKTNKETYMTDIAVTLAALLKIQMPNGSIGKVIEEVLK"
     gene            2377..3636
                     /locus_tag="PLU36_00660"
     CDS             2377..3636
                     /locus_tag="PLU36_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMZ45291.1"
                     /translation="MADIFEKLIKNYGPIGQHRERAHGYFAFPKLEGPISSRMNFRGK
                     EVIVWSLNNYLGLANHPAIRKVDAEASEQWGLAYPMGARMMSGNSTHHEKLEAELAAF
                     EHKEDAILLNYGYQGIMSTIDALCGRHDVIVYDAESHACIIDGLRLHPGHRYVFKHND
                     VEDCEKQLERATALIDKQKTGGILVITEGVFGMAGDQGKLTEIVAFKNKYDFRLLVDD
                     AHGFGTLGKTGAGAGEEQGCQDGIDLYFSTFAKSMASIGAFLAGPRIIIDYIRYNIRS
                     QIFAKSLPMPIVIGNLKRLDMLRTMPELKEKLWHNATKLQKGLKERGFDIGKTDSPVT
                     PIYMKGGVEEATAMVMDLRENYGVFASIVVYPVIPKGHIIYRLIPSAAHTDEDIEITL
                     KAFSATKEKLDAGAYKVDVIPDMAEKK"
     gene            3883..6999
                     /locus_tag="PLU36_00665"
     CDS             3883..6999
                     /locus_tag="PLU36_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HMZ45292.1"
                     /translation="MRKFASMLFVLMLFCTLAFSQNRTITGTVNGNDGKPISGASVTI
                     KGKKGGTVTNDEGHFSISAANGDRLVFSAVGYSAKEVIVNTTSASPVSITLVQEVSNL
                     TEVIVTGVATATPKAKMTVSVTKLSADKINAVNPVSLSSALTGKVAGIKTSNSGGLPG
                     QQVDIQLRADNNLNNVGSGPLIVVDGVQLSGSLADINADDVESIEVVKGAAASALYGS
                     RAGNGVIAVTTKRGKGSGVNIVVRNEVGIQKLAHTLKVATHHPYKLANDWANFKGVFT
                     KYDGVTYPNGYSGTGFHPGIAGNRTQDADHYMDNSYGIERNPQKDVFGTGTNYTNFIS
                     VSSKSEKTGIYASFENNGQQGVVNLTDGYKRQNFRFNLDQQLASWVKFTASNLFVNRT
                     IQYPGTSSSRNSLFYDVARLETDINLSEKNPVDGQKYYPRIDQWIGEITNPMYTLWKR
                     KVNDKSRRWIANYALNFKLNSWANIDVSHTMEIENYRRSTIDPKDTWKVSGGTPATYG
                     MAYTNGGLAMFSSEDNQQNTQFTVNLMHRFGDLNVAGKLSYLYENNHYESFNVGASQY
                     VINDMEGFNNFASISSAGSYISNERAQNYFAIASFDYKDRYLLDAMFRYDGSSLFGSD
                     ARWNPYYRLSGAYRLTKDVTMKGIDEFKIRLAHGTAGIRPGFDWQYEVYTLSNGVATP
                     TQKGNKLLKPSKTTETEFGVEVNFLKRFNFEAIYSFSNTTDQFLNAPLIPFLNDGFPS
                     QWQNAGNVKSNTIEITLGANWKNTPNLKWNSSLVFSRVRQKIGTLPIPPYMFTNQNMG
                     DQYIFRVTSNEAYGAMYGYRFVRTLAEMANQLPTGKTIADYEVNSEGYVIDKGTQGTV
                     NERPIKYKENGADWYGKIGDGNAKFNIGITNTITWKDFSFYFLLDWKQGGDIYNGKDQ
                     RLAFNNMSIRQDMSGVAANEKKAYDYWFSGAYDGNNANQYWVEDGSYLKIREISIGYN
                     LNNKILKKIFNGAIKGVNAKIVGRNLFTFTKYSGYDPEVGTIRQPYDGIYMNPNFRNY
                     AFSLSFNF"
     gene            7019..8497
                     /locus_tag="PLU36_00670"
     CDS             7019..8497
                     /locus_tag="PLU36_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45293.1"
                     /translation="MKKNNIVVFLFLILLAATGCKKSLLDVRNENDPDFKSVLSNGSD
                     VETLTSSLFNTIYDGEHSYSGCSMMLATAADHVSCSWGNAGMRDMSYEPRNNAWNNSS
                     AYSNKGFTKYTFDKMYATINTASQIMKALSNGVVVDNPAKIKAFAKYAQGVAYGNLAL
                     IYDRAFITDENVTVPGELASAVDYKQVATAAIGYLNEAITQSANTFTIPASWLGTAAD
                     LTNADFKALCNTMAARILAYTPRNKAELATVNWAQVKTYADAGITFDFNVMNDNYVKW
                     YDEAGDYLVYNGWGISDTYTINMMDPTLPQHWDDSPSFPYPAASVSPIDQRLNTDFEY
                     VAAQWLNPARGYYHFSPYRNKRYDAMYVNATGAKPEVMKAENDMLRAEARAYTADVAG
                     AAAIINLSTRKTRGNMADVAAVLADVVKAIHHERFVEMYTTGIGLQFFEMRKLDLLQK
                     GTPLHLPLPAGTLETLNAPLPFYTFGTVAKADGTGTSNGGWR"
     gene            complement(8612..9757)
                     /locus_tag="PLU36_00675"
     CDS             complement(8612..9757)
                     /locus_tag="PLU36_00675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ45294.1"
                     /translation="MSSTVIKKVIIIGPAHPLRGGLASFNERLAKQFNQENIETTIYT
                     FSLQYPNFLFPGTTQYSSEPAPKNLDINVCINSINPFNWLKIGWQLKKAKADIIVVRY
                     WLPFMGACLGTILRIVKKNNKTKIVCIADNILPHEKRIGDKQLTSYFLKPIDAFITMS
                     EKVMKDLRMFTNKNAVKVEHPLYDNFGEAIDKKTARQILGLNTNDKIILFFGFIRKYK
                     GLDLLFEAMKILQVKYGHLNIKLLIAGEFYENRKEYDEQINSLNIASNLILKTDFIAD
                     SEVGYYLSAADFVIQPYKNATQSGVTPLAYHFNKPMLVTNVGGLPALVPDKKVGLIAE
                     PNSNDIAEKIVALYEFGEEYFIPYLQEEKKKYSWQNLTNTIIKLAQK"
     gene            9956..10645
                     /locus_tag="PLU36_00680"
     CDS             9956..10645
                     /locus_tag="PLU36_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HMZ45295.1"
                     /translation="MNIEQEFKKFAIKNQGISSGVLHNYQTTFSPTNLTPYIIEERPL
                     NIAQMDVFSRLMMDRIIFLGESINDYVANIITAQLLFLESADRTRDIQMYINSPGGSV
                     YAGLGVYDTMQFISPDVSTICTGIAASMGAVLLCAGVQGKRTALKHSRIMLHQPSGAI
                     GGQATDIGITAKEIKILKQELYEVIAHHSGKDIETVSKDCDRDFWMKAIDAKAYGLVD
                     EVLFTNPKKEK"
     gene            10678..11316
                     /locus_tag="PLU36_00685"
     CDS             10678..11316
                     /locus_tag="PLU36_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HMZ45296.1"
                     /translation="MLSSNKIINPFKLEDEEEDNKKDEKQEQLSPGMMKKMEKLFFEK
                     RAVYLWGVVEDKSIREVVSKILLLEADKPGKEIKLYINSPGGVVTSGMVLFDTMTMIS
                     SPVSTICMGLAASMGSILLCAGTKGKRYIYPHGEVMIHQPSISGYYQATSADIEIQAI
                     QIEKTKLASAKILAKNCGKTVEQVLADFDRDYWMDAQEAVNYGIVDEILHKL"
     gene            11443..12687
                     /gene="clpX"
                     /locus_tag="PLU36_00690"
     CDS             11443..12687
                     /gene="clpX"
                     /locus_tag="PLU36_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HMZ45297.1"
                     /translation="MAKQSHLHCSFCGRSRDEVKILIAGQEGHICESCVEHALEIIEQ
                     EIHTSDKSKSNTNLNTKLTIRKPQEIKKFLDEYVIGQDEAKKILAVAVYNHYKRISHK
                     QLPDEVEIEKSNIIMVGETGTGKTLLAKSIARMLNVPFAIVDATVFTEAGYVGEDVES
                     MLTRLLQNCNYDVAAAEKGIVYVDELDKIARKGDNPSITRDVSGEGVQQGLLKMLEGT
                     EVLVPPQGGRKHPEQKMIKIDTKNILFICGGAFDGVDKVIARRINTNAIGFNVNKEEQ
                     ELQRNNLLRFVNAQDLKKFGLIPELLGRLPVVTYLNPLDAATLRNILTEPKNSIIKQF
                     TKLFELEGIKLKIDDEVLDFMVSKALEYKLGARGLRSICESIFTDAMFEMPGTNKTEL
                     HITANYAENMFNKSKLNLLKVA"
     gene            12827..13438
                     /locus_tag="PLU36_00695"
     CDS             12827..13438
                     /locus_tag="PLU36_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HMZ45298.1"
                     /translation="MKKSDFNLQVQNDNIESKIVASLERIAQAFRVLLWQETKEFSLS
                     PIQVQILIFLLNHSKDKCKVSYLAAEFNLTKATISDTIKTLEQKKLIEKEYEVEDSRS
                     YIIHLTQKGKEIAQKTSFFTNEIKSPISKLSSENKEHLYCSLLDIIKYLNTQKIVTIQ
                     RSCINCTHYQTNKNGLKHFCKLLNSNLSTSQVRIDCPLHQEKL"
     gene            13638..15242
                     /gene="pckA"
                     /locus_tag="PLU36_00700"
     CDS             13638..15242
                     /gene="pckA"
                     /locus_tag="PLU36_00700"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HMZ45299.1"
                     /translation="MSIPSFSIPTEKLLTLGISDVTKVHYQLSPSELVQQALDRKQGV
                     LNSTGALCINTGEFTGRSPLDKFVVKDNITANSVHWNNFNIPIEDKYYQQLKRKVLNY
                     LSQQGEIWVRDAYACASPEHRINIRVINENPWSNLFAYNMFLRPTEAELSNYPVDWHI
                     IQAPGFRADASIDGTRQHNFAIVSFTEKTILIGGTGYTGEMKKGIFTILNFVLPHDKN
                     VLSMHCSANMGKDGDVAIFFGLSGTGKTTLSADPNRKLIGDDEHGWTNDSVFNFEGGC
                     YAKVIDLSAEKEPEIFNAIRPGAILENTTFLDGTNEIDFASKKITENTRVSYPLNFIS
                     NALEPSIGGLPKNIFFLTCDAYGVLPPISKLTPGQAMYQFISGYTAKVAGTEAGVTEP
                     KSTFSACFGAPFLPLHPGFYAEMLGKKMKENKVNVWLVNTGWSGGGYGVGQRMKLSYT
                     RAMITAALNGELNNVVFEKHPIFGVEMPTTCPNVPENILNPKNTWNDKEAYDAAAKNL
                     AAQFIKNFEKYAAGVTEEILASAPKA"
     gene            complement(15467..16030)
                     /locus_tag="PLU36_00705"
     CDS             complement(15467..16030)
                     /locus_tag="PLU36_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HMZ45300.1"
                     /translation="MNQQYILNTIQLFEYYKSLGDKTFNQLQEKDLFWQYNNNSNSIA
                     IIVKHLCGNMLSRWTDFLTTDGEKEWRKRDEEFENDLKNKEELLHKWKEGWDCLFKAL
                     NSLTEKDLNNTVYIRNQGHTVTDAINRQLAHYASHIGQIMYIGKMIKDADWQSLSIPK
                     GNSQQFNQEKFAKEKHDEHFTNEALGK"
     gene            16152..16985
                     /locus_tag="PLU36_00710"
     CDS             16152..16985
                     /locus_tag="PLU36_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HMZ45301.1"
                     /translation="MIEVGKFNTLVINRKVDFGFYLDNGAEGILLPKRFAPANAKIGD
                     TVNAFIYHDSDNRLIATTQQPNGVVGDIVLMKCVSTMSAGAFLDWGLMKDLFVAKSQQ
                     KVGMKVNGWYLVKIYLDVQTGRVAASEKIEKYLDNSDLTVAEKEEVDIVIYRKTDIGY
                     SCIINNRHYGLIHNNQVFQNLEVGDLLKGFVQTIREDNKIDIVLGKVGYKKTEDEVEK
                     IIRLLKESNGYLPYHDKSEPDEIYNYFGMSKKTFKMAVGALYKQRKINFAKEGIILRE
                     Q"
     gene            17007..18272
                     /locus_tag="PLU36_00715"
     CDS             17007..18272
                     /locus_tag="PLU36_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HMZ45302.1"
                     /translation="MKFLNLLFVLFTANFLYAQNKVVDTNQVVTPNRVNTEQTLQKPY
                     VILISADGFRYDYFKKYKAKNLLNIAAKGVWAKKGMHPSYPSITFPNHYSIITGLYPS
                     HHGIVDNIFYDSIRKETYKIGSKTVIDGTWYNGLPLWALAEKQGMLSASLFWVGSESN
                     AANTRPTYWYHYHEKFKDDAKVNIIKNWLTLPDSIRPHFITLYFPEVDYYGHKFGPDA
                     KETEASVHYIDVAIKKLVDSLQPLNLPINFIFVSDHGMIAVEEKDYIPLPEIDSKKFT
                     VINSYTLVRVTAKDKLDVLPLYQKLLNEKTDDYNVYLANNFPEKLNYSSREDTIGRIG
                     DILLVPVGSKLLVNKNKKPTYGKHGFNSYEIPEMKASFIAWGENFKKGKKIKTFQNIH
                     IYPLVASILDLEILQPIDGSLKVLQKTIR"
     gene            complement(18297..18719)
                     /locus_tag="PLU36_00720"
     CDS             complement(18297..18719)
                     /locus_tag="PLU36_00720"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HMZ45303.1"
                     /translation="MSELKTKKNNASVIDFINLVEKEQKRKECFQLLELFETVTKEKA
                     SMWGSSIIGFGEYKYKYESGREGDWFFAGFSPRKQNIVIYIIAGFTDYESLMQQLGKY
                     KTGSSCLYINKLADIDLKVLKLLIEKSFAFMKNKYAKN"
     gene            complement(18731..19915)
                     /locus_tag="PLU36_00725"
     CDS             complement(18731..19915)
                     /locus_tag="PLU36_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45304.1"
                     /translation="MKLFTVFLVILFVSCNGQSVLEVSKENSNTVGGGCEGCELMYVE
                     IPDEISNEHTSLGWFEGKQKLIITGTVFQLDGKTPAANTLIYYWHTDDAGLYSSNEQT
                     SEKVKAHGKLRGWIKTDSSGKYTIKTSRPASYPNEDIPQHIHLSIKEPNITNEYFADL
                     YFDDDKLYLAHKKKYGKIDRAGSEILRVLIDNEVQIAEHDIILGLNIPHYPSLKQSNN
                     LSGLNIGEDQPSFTPYHAFGADKGTQTCPVCKYGRYHGIIYFVGNHPNWNEIKTWLIF
                     LDNESRLREKYLKTYFVLGNEKEFIHENTFTFLESLGKELNLQNIALTFVPSFNDSST
                     EVNLNKIDAEAKNTIIIYKHRTIVDKYINLLPTKENFAKIINVLDKTKGNYFNLEEPK
                     HD"
     gene            20091..20267
                     /locus_tag="PLU36_00730"
     CDS             20091..20267
                     /locus_tag="PLU36_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45305.1"
                     /translation="MLLLLQNFSEIEKELFLKNESEMQDNFLSMLHYFSKANTNNDFP
                     LLRSGLVLSGVNNY"
     gene            20280..20633
                     /locus_tag="PLU36_00735"
                     /pseudo
     CDS             20280..20633
                     /locus_tag="PLU36_00735"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
     gene            20729..22225
                     /gene="guaB"
                     /locus_tag="PLU36_00740"
     CDS             20729..22225
                     /gene="guaB"
                     /locus_tag="PLU36_00740"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HMZ45306.1"
                     /translation="MATIKPSLSKNKVNRVFIDGLTFDDVLLLPAYSQVLPREVNLHS
                     HLTKEIVLNIPLLSAAMDTVTEANLAIALAREGGLGILHKNMSIEKQAEQVRKVKRSE
                     SGMIIDPLTLTTNSTIGDALKLMKENKIGGIPIIDNQGKLVGILTNRDLRFETDKKRK
                     VTDVMTNEKLITAPEGTDLKKAEIILRKYKIEKLPVVNKQGKLVGLITYRDILQLTNH
                     PNAVKDNYGRLLVGAALGITNDLMQRADALMNVGVDIVTLDSAHGHSKGVIEALKALR
                     KNFKKLQIIAGNVGTAEGALALANAGADCVKVGIGPGSICTTRIVAGAGVPQLTAVME
                     ASNALKKKNIPVIADGGIRYTGDMVKALAAGANTVMMGSVFAGTEESPGETIIYEGRK
                     FKTYRGMGSLGAMSQGSGDRYFQDVEDDVKKFVPEGIEGRVAFKGNLSEIVYQFVGGL
                     RSGMGYCGAKNIAALQNATFVKITNAGMKESHAHDIDITKEAPNYSRK"
     gene            22380..>24153
                     /gene="thrS"
                     /locus_tag="PLU36_00745"
     CDS             22380..>24153
                     /gene="thrS"
                     /locus_tag="PLU36_00745"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HMZ45307.1"
                     /translation="MINISFPDGAVRQYEAGVSAMDIAKSISEGLAKKVLVAGVNNEV
                     WDASRPITTDSSLKLFTWNDAEGKNTFWHSSAHLMAEAIEAKFPGVKFWVGPALENGF
                     YYDIDLDDRKITEEDLVELEKIMNELAKKNNVYLRTEKAKQEAIAYFTEKGDEYKLDL
                     LQNLEDGTITFYTQGNFTDLCRGPHIPNTGFIKAIKLLNIAGAYWKGDEKNNQLTRIY
                     GITFPTQKELDEYLTMLEEAKKRDHRKLGKELGIYTMNDDIGQGLILWMPNGTIIIEE
                     LEKLAKQSEEAAGYKRVVTPHIAKENLYLTSGHLPYYADSMFAPMEMDGEKYYLRAMN
                     CPHHHKIFDAEPKSYRDMPYRIAEYGTCYRYEQSGELFGLMRVRCLHMNDAHIYCTKE
                     QFAQEFKAVNDLYLKYFKIFGIEKYVMRLSLHDPEKLGQKYVNEPELWLETEELVRNV
                     LKETGVPFVEVKGEGAFYGPKIDVQIWSAIGREFTLATNQVDFAQPRSFKLSFTNQNN
                     EPEIPLIIHRAPLGTHERFIGFLLEHYAGKFPVWLAPLQVKILPISDKFLPYATTVKN
                     ALQQVGVRVEIDDRNEKIGKKIREA"
BASE COUNT         8640 a         3409 c         4178 g         7926 t
ORIGIN      
        1 gataattttc cagatcctga agatgatata ccagtagatg gtggtttaac tttattgtta
       61 gcagcaggag caggtttagg agcaaagaag ttaagagata acagaaagaa acaacagaat
      121 aaaaactaag cagcctgttt aacaacttct tcaattactg taattttaat taattacaat
      181 gtaacctcat taaataataa caaaagcaat aaaaagccct tgcatatatt ttgcaagggc
      241 ttttatatgg tatagggtat attattaaaa gaggagtaaa tttttgtgaa taggtttatg
      301 gtaagattat ttatcattgt tattcaaata aacctaaata gtaatttata aattaaaatc
      361 taccatagtt gagataaata aaaaaatcat ctgcattaca gatgatttta cgaaagtgca
      421 acagtataaa atttatttta aaacttcttc aatcacttta ccaatactac cattaggcat
      481 ttgaattttt aacaatgcag caagagtaac tgcaatatcg gtcatataag tttctttatt
      541 ggttttgcct tgttttattc cgtaaccata ccaaaccaat ggaatgtgtg catcataagg
      601 gttccaggca ctatggtctg tacctttttc tgtatctctc atccattgtg gtaaaggaat
      661 aacttgtaaa tctccacttc ggttaggata gtaaccatta attaaagatt ctttaatttt
      721 aaaaggcaag ccagatgttt ctaatttttg tttatctata actctatata ctccatcttc
      781 tttttctata gaattaataa taaaatcttt tatagtttga agattaattt ttgccgaatc
      841 tatttgagca tgatttaagg aaatgttata gtatgtttgg taagcaatag ctttagaaat
      901 accaaattgt tgagcaattg ttttatttag gtcagaaaca atttttttaa gccctaatga
      961 gccgcctggt attttatgtt tttgtaaata tcctgcaata tgtgctcccc catgatctgc
     1021 agaaagaaat aatgtatatt ggccttttcc aatataagta tctaaatatt gaataaaagc
     1081 tgcaaggtct ttgtctaatc gtaggtacat atcttctgct tcaacagatg ctgggccaaa
     1141 ggaatgtccc atataatcag gggcagataa actaatagct aaaaaatcgg ttactccgtt
     1201 tttgcccata ttttctgcct ttaaagctgc cctagccata tttaaagtgt aggtattgct
     1261 ttgtggcata tagctaaatt ttttatagtc tttactaata tgttgtgaaa atttgtatgg
     1321 aaaaacaggc ttatctactt ctatccaatt ggttctttcg taattgttat catctccttc
     1381 gctttgagtg taagtttcta ttgggtacaa agtattccat ccttcagcat agtacttatc
     1441 tacccagttt tgagcattaa aatctttaac ccatttaggt aaatcgttca tgtaatatgt
     1501 gctgcttata aaaatccctt tggtttcatc gtaccaatag gcagcattgg cactatggcc
     1561 agcaggtaaa atagagcctc tgtcttttaa agcaatacca attactttac ttttaaagtt
     1621 agatgctaac cttaattcgt ctgttatagt attagcaacc atattccttg ggctcattaa
     1681 tcctttttta gagttgcttc caacgccttt tacagtgcta tcatcggtac aatatacata
     1741 ttgttgctgt ttcatatccc accaattatt acttgcaata ccatgtatag ctggtacagt
     1801 gccagtataa gcactagcat ggccacaggc tgttgcagtt ggtgtataag gtataaaagt
     1861 attttcgcaa gtaaagcctt cttttaaaag ctttttaaag ccatcattgg tatatctgtt
     1921 ttgatattta tataaataat cccaacgcat gtgatctatc ataataccta ttaccaattt
     1981 tggttttgaa gtatttggtg tagccgtact ggttgttttt tgtgcaaagg aaagagtagt
     2041 taaaatagat aataggagag tactgaataa tttcataagt tatttattat tatttatggt
     2101 aaatgccaat tatttaataa actggtataa aaagtattta ggctaataga aaaagtttaa
     2161 tttatcacgt tgttatttca tgttttattg tagcaaatgt aataataaac ttactgattt
     2221 gatttattgg tgttatttga taaacattta cctttgcggc aatttttatt ttgttcagca
     2281 atgtaaaaag cgtacaattg tgcgacgcaa caggagctaa ataatcattc tcctgccaag
     2341 taaataatat gttttttaaa attttaactt aattcaatgg cagacatttt tgaaaaatta
     2401 attaaaaatt atgggccaat tggtcagcat agagaaagag ctcatggata ttttgctttt
     2461 cctaaattag aaggtcctat tagcagtaga atgaacttta gaggtaaaga agtaatagtg
     2521 tggagtttga ataattattt aggattagct aaccatcctg caattaggaa agtggatgca
     2581 gaagcatcgg aacaatgggg gcttgcttac ccaatgggtg caagaatgat gagtggaaac
     2641 tctacccatc atgaaaaatt agaagcagag ctagcagctt ttgaacataa agaagatgct
     2701 attttattga actatggcta tcaaggaata atgagtacta ttgatgcatt gtgtggaagg
     2761 catgatgtaa ttgtatatga tgctgaaagc catgcttgta ttattgatgg tttgcgttta
     2821 caccctggcc acagatatgt gtttaaacat aacgatgttg aggattgtga aaaacaattg
     2881 gaaagagcaa ctgctttaat tgataaacaa aaaacaggcg gtattctagt gattactgaa
     2941 ggtgtttttg gaatggcagg agaccagggt aaactaacgg aaattgtagc ctttaaaaat
     3001 aaatatgatt ttagattatt agtagatgat gcacatggtt ttggtacatt aggaaaaact
     3061 ggtgcaggtg ctggcgaaga acaaggctgc caggatggta tagatttata ctttagcact
     3121 tttgcaaaaa gcatggctag tattggtgca tttttagctg ggccaagaat aattatagac
     3181 tatataagat acaatatacg tagccaaatt tttgcaaaaa gtttacccat gccaattgta
     3241 attggtaact taaaaagatt ggatatgctg cgtacaatgc cagaattaaa agaaaaatta
     3301 tggcacaatg caactaagct acaaaaaggg ttaaaagaaa gaggatttga tataggtaaa
     3361 acagatagcc ctgttacacc tatttacatg aaaggcggtg tagaagaggc aactgcaatg
     3421 gttatggatt taagagaaaa ttatggagta tttgctagta ttgttgtata tcctgtaata
     3481 cctaaaggac atattattta tagattaatt ccaagtgctg cacataccga tgaggatatt
     3541 gaaataacat taaaagcatt tagtgcaact aaagaaaaat tagatgcagg tgcttacaag
     3601 gtagatgtaa tacctgatat ggctgaaaag aaataaaaac tttacaaata ctttttatca
     3661 agggggtagt tatttaacta cctccttttt ttggtatttg ttagtaacaa aaaactaact
     3721 gggattattt tttttaaaaa aagttaaaaa ttttttgcac tatttgttaa aataattata
     3781 attttaataa tcattataaa gatgatagag tatttttttc aatctaaact tttaaagaat
     3841 taaactctgg tatctttttt ttaaaattaa aactaacatt acatgagaaa atttgcatct
     3901 atgttgtttg tgctaatgtt gttttgtaca ttagcattct ctcaaaacag aaccattaca
     3961 ggtactgtaa atggcaacga cggaaaaccc atttctggag caagtgtaac cattaaaggc
     4021 aaaaaaggag gaactgtaac taatgacgaa ggtcattttt ctatttctgc agcaaacgga
     4081 gatagactag ttttttctgc tgtagggtac agtgcaaaag aagttattgt taacactaca
     4141 agtgcaagcc ctgtttctat tacactcgtt caagaagtgt caaaccttac tgaagtaatt
     4201 gtaactggtg tggcaactgc aactccaaag gcaaaaatga cggtatctgt tacaaaatta
     4261 tctgcagata aaattaatgc agttaatcct gtttccttat cctctgcctt aacaggtaaa
     4321 gttgctggta ttaaaacttc caactctgga ggcttacctg gtcagcaagt agatattcaa
     4381 ctacgtgcag ataataactt aaataatgtt ggctctggtc cgttaattgt tgtagatgga
     4441 gttcagttat ctggaagcct tgcagatatt aatgcggacg acgtagaatc tattgaagtt
     4501 gtaaaaggtg ctgcagcatc tgctctttat ggttcaagag caggtaatgg tgtaatagca
     4561 gtaacaacta aaagaggtaa aggatctggt gtaaatattg tagtaagaaa cgaagtaggt
     4621 attcaaaaac ttgcacatac attaaaagta gcaacccatc atccttataa attagcaaac
     4681 gattgggcaa actttaaagg tgtatttaca aaatacgatg gagttactta tcctaatgga
     4741 tatagtggta caggatttca tcctggtatt gcaggcaaca gaacacaaga tgcagaccat
     4801 tatatggata attcttatgg tatagaacgc aatccacaaa aagatgtttt tggtacagga
     4861 actaattata ccaactttat ttctgtaagc tccaaatctg aaaaaacagg tatttatgca
     4921 tcttttgaaa acaacggaca acaaggtgta gtaaacctaa ctgatggtta taagagacaa
     4981 aactttagat ttaatcttga ccaacagctt gcaagttggg taaaattcac tgcatctaat
     5041 ttatttgtaa atagaaccat tcaatatcct ggtacaagct cttctcgtaa cagtttattt
     5101 tatgatgtgg ctcgtttaga aacagatatt aatttaagtg aaaaaaatcc ggtagatggt
     5161 caaaaatatt atccaagaat agaccaatgg attggtgaga ttaccaaccc aatgtacaca
     5221 ctttggaaaa gaaaagtaaa tgataaaagc cgtagatgga ttgccaatta tgcattaaac
     5281 tttaaattaa atagttgggc aaatattgat gttagccaca ctatggaaat tgaaaactat
     5341 cgtagatcaa ctattgatcc aaaagacact tggaaagttt ctggtggtac acctgcaacg
     5401 tatggtatgg cttacaccaa tggtggttta gcaatgtttt cgtcagaaga taatcaacaa
     5461 aatacccaat ttacagtaaa cttaatgcat cgctttgggg atttaaatgt ggcaggaaaa
     5521 ttatcttatt tatatgaaaa taatcactat gaaagcttta atgttggtgc ctctcaatat
     5581 gtaattaatg atatggaagg gttcaacaac tttgcttcaa ttagtagtgc aggctcttat
     5641 ataagcaatg aaagagcaca aaactatttt gcaattgctt ctttcgatta taaagacaga
     5701 tatttattag atgccatgtt ccgttatgat ggttcttctt tatttggttc tgatgcaaga
     5761 tggaatcctt attatcgttt gtctggtgca tacagattaa caaaagatgt aactatgaaa
     5821 ggaatagatg aatttaaaat aagattagca cacggaactg caggtattag accaggtttt
     5881 gactggcaat atgaagttta tactttaagt aatggagtag caactccaac acaaaagggt
     5941 aataaattat taaaaccatc taaaactacc gaaactgaat ttggtgtgga agtaaacttt
     6001 ttgaaaagat ttaatttcga agctatttat tcattctcta atactactga tcaattctta
     6061 aatgctccat taattccttt tttaaatgat ggttttccaa gccaatggca aaatgcaggt
     6121 aatgtaaaat caaataccat tgaaattact ttaggtgcaa actggaaaaa cactccaaat
     6181 cttaaatgga attcttcctt agtattttca agagttcgtc aaaaaattgg cactttacca
     6241 attcctccat atatgtttac caaccaaaat atgggcgacc aatatatttt ccgtgtaaca
     6301 tctaatgaag cttatggggc aatgtatggt tacagatttg tacgcacttt agcagaaatg
     6361 gcaaatcaac ttccaactgg taaaactata gccgattatg aagttaatag cgaaggatat
     6421 gttatagata aaggtaccca aggtacagta aatgaacgcc ctattaaata taaagaaaac
     6481 ggtgctgatt ggtacggaaa aattggagat ggaaatgcta aatttaatat tggtattact
     6541 aacaccataa cttggaaaga tttttcattt tatttcttat tagattggaa acaaggcggc
     6601 gatatttata atggtaaaga ccaacgctta gcatttaata atatgtctat ccgtcaagat
     6661 atgagtggtg ttgcagctaa tgagaaaaaa gcttacgatt attggttctc tggagcttat
     6721 gatggtaaca atgcaaatca atattgggtg gaagatggaa gttacttaaa aattcgtgaa
     6781 atatctatag ggtataattt gaataataaa atattgaaaa aaatattcaa tggtgctatt
     6841 aaaggtgtaa atgcaaaaat tgttggtaga aacctattta catttacaaa atactctggc
     6901 tacgacccag aagtaggtac tattagacaa ccttatgatg gtatttatat gaatcctaat
     6961 tttaggaact atgcattttc gttatctttc aatttctaat ttttaaaaat tacaaaatat
     7021 gaaaaaaaat aatattgtag ttttcttatt tcttattctg ttagcagcaa ctggttgtaa
     7081 aaaaagcctg ctagacgtta gaaatgaaaa cgatcctgat tttaaaagtg tgctttccaa
     7141 tggaagtgat gtagaaacct taacttctag tttatttaat acaatttatg atggagaaca
     7201 cagttatagt ggttgctcta tgatgcttgc tactgctgcc gatcatgtat cttgctcttg
     7261 gggtaatgca ggtatgagag acatgtctta tgagccacgt aacaatgcat ggaacaactc
     7321 ctctgcatat agcaataaag gctttactaa atacactttc gataaaatgt atgcaacaat
     7381 taatactgca tctcaaatta tgaaagcatt aagtaatggc gttgtggtag ataaccctgc
     7441 aaaaattaaa gcctttgcaa aatatgcaca aggcgtagct tatggtaacc ttgcattaat
     7501 ttatgatagg gcatttatta cagatgagaa tgttacagtt cctggcgaac ttgcaagtgc
     7561 tgtagattat aagcaagttg ctactgccgc aattggttac ttaaacgaag ctattaccca
     7621 aagtgccaat acttttacaa ttcctgcaag ctggcttgga actgcagcag atttaaccaa
     7681 tgcagatttt aaagcattat gcaataccat ggctgccaga attcttgctt atacacctcg
     7741 taataaagca gagttggcta cagtaaattg ggctcaagta aaaacctatg cagatgctgg
     7801 tattactttt gattttaacg taatgaacga taactatgtt aaatggtacg atgaggcagg
     7861 agattatctt gtttacaatg gttggggaat ttctgataca tatacaatta atatgatgga
     7921 cccaacatta ccacaacatt gggatgactc tccttcattc ccttaccctg cagcttctgt
     7981 ttctccaata gatcaaagat taaatacaga ttttgaatat gtagcagcac aatggcttaa
     8041 ccctgcaaga ggttattacc atttttctcc ttatagaaat aaaagatacg atgctatgta
     8101 tgtaaacgca actggtgcaa aaccagaggt aatgaaagct gaaaatgaca tgttacgtgc
     8161 agaagcaaga gcttatactg ctgatgtagc tggtgctgca gcaattatta atttaagtac
     8221 acgtaaaaca agaggtaata tggcagatgt tgctgctgta cttgcagatg tagttaaagc
     8281 aatacatcac gaaagatttg ttgaaatgta cacaactggt attggtcttc aattttttga
     8341 aatgagaaaa ttagacttat tacaaaaagg tacgccttta cacttgcctt taccagcagg
     8401 tactttagaa acattaaatg caccattgcc attttatact tttggtactg ttgcaaaagc
     8461 agatggtaca ggtacatcaa atggtggatg gagataagat agtgttgtaa aataaaattt
     8521 aaaaaggagt tgtaaaatac aactcctttt taaataataa atagtcaaaa agttggtaac
     8581 tacaattata aaagtattaa taaaacacat tttatttttg tgctaattta attatagtat
     8641 tggtaaggtt ttgccaacta tatttcttct tttcttcctg taagtagggt ataaaatatt
     8701 cttcaccaaa ttcgtaaaga gctacaattt tttcagctat atcatttgaa tttggttcgg
     8761 caattaaacc aacttttttg tcaggtacca aggctggtaa ccctccaaca ttagtaacca
     8821 acatgggttt attaaaatga taagccaatg gggttacacc actttgagtt gcatttttat
     8881 aaggttgtat tacaaaatct gctgcactta aataatagcc aacttcgcta tctgcaataa
     8941 agtctgtttt aagaataagg tttgaagcaa tgtttaatga atttatttgt tcatcatatt
     9001 cttttctatt ttcataaaac tcacctgcaa taagtagttt aatatttaaa tggccatact
     9061 ttacttgcaa aattttcatg gcttcgaata ataaatctaa ccctttatat tttctaataa
     9121 atccaaaaaa taaaattatt ttatcattgg tatttaagcc taagatttgc ctagctgttt
     9181 ttttatctat tgcttctcca aaattatcgt ataaaggatg ctcaacttta acagcatttt
     9241 tattggtaaa cattcttaaa tctttcatta ctttttcact catagtaata aaagcatcta
     9301 ttggctttaa aaaataactg gttaactgtt tatcgccaat tcttttttca tggggtaaaa
     9361 tattatcagc tatacaaaca atttttgttt tattattttt ttttacaatt cttaaaatgg
     9421 ttcctaagca agctcccata aaaggcagcc aatagcgtac aacaataata tctgctttgg
     9481 ctttttttaa ttgccatcct attttaagcc agttaaaggg gttaatagag ttgatacaaa
     9541 cattgatatc taagtttttg ggagcaggtt cagaagaata ttgtgttgtg cctggaaata
     9601 aaaaattagg atattgtaaa gaaaaggtat aaatggttgt ttctatattt tcttgattaa
     9661 actgtttggc aagcctttcg ttaaaagaag ctaagccacc tcttaaaggg tgagcagggc
     9721 caataataat tacttttttt ataactgtag aagacacttt attgtcaata tttttttcaa
     9781 ataaatcaaa gataataatg catagttgtt ataccataat ttactaaaaa cagcaatgtg
     9841 tattaaatta tattattact atgccctttt gtcggttttt gcttttttgc atattatttg
     9901 gataaaaatt ttatcaaaat ttcaaaacac ttatcttcaa cgcaaaatta atagcatgaa
     9961 tatagaacag gaatttaaaa aatttgccat aaaaaaccag ggcataagca gtggggtttt
    10021 gcataattat caaacaactt tttctccaac taatttaaca ccatatatta ttgaagaacg
    10081 cccattaaat attgctcaaa tggatgtgtt tagtaggcta atgatggata gaattatttt
    10141 tttaggagaa agtattaacg actatgtagc aaatattata actgcacaac ttttattttt
    10201 agaaagtgca gatagaacaa gagatattca aatgtatata aatagcccag gtggtagtgt
    10261 ttatgcaggt ttgggagttt atgatacaat gcagtttata agcccggatg taagtacaat
    10321 ttgtacaggt attgcagcaa gtatgggtgc agtattactg tgtgcaggtg tacaaggtaa
    10381 gagaaccgct ttaaaacata gtcgtattat gctgcatcaa ccaagtggag ccattggtgg
    10441 gcaagcaaca gatattggta taacagcaaa agaaataaaa attttaaaac aagaattgta
    10501 tgaagtaatt gctcatcatt caggaaaaga tattgaaact gtttctaaag attgtgatag
    10561 agacttttgg atgaaagcca tagatgccaa agcttatggt ttggttgatg aagtactttt
    10621 caccaatcct aaaaaagaaa aataactttt ttcattccgt aaattcaaaa ataatatatg
    10681 ttaagtagca ataaaataat aaaccctttt aagttagaag acgaagaaga agataataaa
    10741 aaagatgaaa aacaagagca gttaagccca ggaatgatga agaaaatgga gaaacttttt
    10801 ttcgaaaaaa gagctgtgta tttatggggc gtggtagagg ataaaagtat aagagaagta
    10861 gtaagtaaaa ttttattgtt agaggcagat aaacctggca aagaaattaa gctttatatt
    10921 aatagcccag gaggggttgt aaccagtggc atggttttat ttgatacaat gacaatgata
    10981 agcagccctg ttagtacaat atgtatgggt cttgctgcta gtatgggttc cattttacta
    11041 tgtgcaggta ctaaaggcaa aaggtatata tatccacatg gtgaggtaat gatacaccaa
    11101 ccaagtataa gtggctatta tcaagcaact agtgccgata tagaaattca ggcaattcaa
    11161 atagaaaaaa ccaaattagc tagtgctaaa atattagcaa aaaactgtgg caaaaccgtt
    11221 gagcaagttc ttgcagactt tgatagagat tattggatgg atgcacaaga agcagttaat
    11281 tacggcattg tagatgaaat tttacataag ctttaaaact attttaatac tttttttata
    11341 ctcattggta taatttaatt tacatttgta cattaatttt ataagtattt acgttatact
    11401 tgtactaatt aatctattaa taacatctac atttaattaa taatggcaaa gcagagtcat
    11461 ttacattgct cattttgtgg tcgtagcaga gacgaggtga aaattttaat tgctgggcaa
    11521 gaagggcata tttgcgaaag ttgtgtagag catgctttag aaattattga acaagaaatt
    11581 catacttcag ataaatctaa aagtaatacc aatcttaata caaaacttac catacgtaaa
    11641 cctcaagaaa ttaaaaagtt tttagacgaa tatgtaatag gacaagacga ggcaaaaaaa
    11701 attctggcag ttgctgttta taatcattat aaaagaattt ctcataaaca attacctgat
    11761 gaggttgaaa ttgaaaagag taatataata atggttggcg aaactggtac aggcaaaaca
    11821 ttacttgcaa aaagtattgc ccgtatgcta aatgtaccat ttgccattgt agatgctact
    11881 gtttttactg aggctggtta tgtaggagaa gatgttgaaa gtatgctaac acggttattg
    11941 caaaattgta attacgatgt tgctgctgcc gaaaaaggta ttgtatatgt agatgaatta
    12001 gataaaatag cacgtaaagg agataaccct tctattacaa gagatgtgag tggagaaggt
    12061 gtacaacaag ggttattaaa aatgttagaa ggaaccgaag tgttagttcc accacaaggt
    12121 ggtagaaagc atcctgaaca aaaaatgatt aaaatagata ctaaaaatat tttatttatt
    12181 tgtggaggtg catttgatgg tgtggataag gttatagctc gccgtataaa taccaatgct
    12241 attggattta atgtaaacaa agaagaacaa gaattacaaa gaaataattt actacgattt
    12301 gtaaacgctc aagatttaaa aaagtttggc ctaatacctg aattattagg tagattacct
    12361 gttgtaactt atttaaaccc tttagatgct gccactctac gaaatatttt aactgaaccc
    12421 aaaaactcta ttattaagca gtttacaaaa ttatttgaat tggagggaat taagttaaaa
    12481 atagatgatg aggtactaga ttttatggta agcaaagcat tagaatacaa attaggggct
    12541 agaggcttaa gaagtatttg cgaaagcata tttaccgatg caatgtttga aatgcctggt
    12601 accaataaaa cagaacttca tattactgct aattatgcag agaacatgtt taataaaagc
    12661 aaactcaatc ttcttaaagt tgcataagta gcaatttttt cactaaattt taattgttat
    12721 tttaagctaa attatttttc ccatatagtt aggataacta cctttatggg actttttctt
    12781 ttaaactgta ttttttgcag aaactatttt agcgtaataa taaataatga agaaatctga
    12841 ttttaactta caagttcaaa atgataatat tgagagcaaa attgttgctt ctttagaaag
    12901 aattgcacaa gcatttcgtg ttttactttg gcaagaaact aaagaatttt ctttaagtcc
    12961 aatacaggta caaattttaa tttttttatt aaatcattca aaagataaat gtaaagtaag
    13021 ttacttagct gccgaattta accttacaaa agctacgata agcgatacca ttaaaacgct
    13081 tgagcaaaaa aaattaattg aaaaagaata tgaagtagaa gatagcagaa gctatataat
    13141 acatttaaca caaaaaggta aagaaatagc tcaaaaaact tcttttttta ccaacgaaat
    13201 aaagtcgcca atttcaaaac tctcttcaga aaataaagaa catttatatt gtagcttatt
    13261 agatataatt aagtatttga atacccaaaa aattgtaacc attcaaagaa gttgtatcaa
    13321 ctgtacccat tatcaaacta ataaaaacgg cctaaaacat ttttgtaaat tattaaactc
    13381 taacttatct acctcacaag taagaataga ctgtcccttg caccaagaaa aattatgaac
    13441 gtaaacacct ataaaactgg atgcacaaaa tagaatagaa aataaatagt aattatttga
    13501 ttttcagtat ttaataatgg cattaaaatt attaaaattt atttttaact aagaatgtaa
    13561 acatataatg ccctacaaaa aatattttgg atattgataa tccttatttt tgcaaactaa
    13621 caaatgtttg tttaaatatg tcaattccat ccttttcaat tccaacagaa aaattattaa
    13681 cgttaggaat ttcagatgta actaaagttc attatcagct atccccatca gaattagtac
    13741 aacaagccct agatagaaag caaggtgttt taaatagtac tggagcttta tgtattaata
    13801 ctggtgaatt tacaggacgt agcccacttg ataaatttgt agttaaagac aatattactg
    13861 ctaactctgt acattggaat aattttaata ttcctattga agataaatac tatcagcagt
    13921 tgaaaagaaa agtattaaat tacttaagcc agcaaggaga aatttgggta agagatgctt
    13981 atgcttgtgc aagcccagaa catagaataa atattagggt tattaatgaa aacccttgga
    14041 gtaatttatt tgcttacaat atgtttcttc gcccaacgga ggcagaatta tccaattatc
    14101 ctgtagattg gcatattatt caagctcctg gctttagagc agatgctagt atagatggta
    14161 cacgccaaca taattttgca atagtttcat ttacagaaaa aacaatttta attggtggta
    14221 caggttatac tggtgaaatg aaaaaaggaa tttttaccat tttaaacttt gttttaccac
    14281 acgataaaaa cgtattaagc atgcattgca gtgctaatat gggaaaagat ggtgatgtgg
    14341 ccatattttt tggattaagt ggaacaggaa aaacaacttt aagtgctgat ccaaatcgta
    14401 aattaatagg agatgatgag catggctgga ccaatgacag tgtatttaat tttgaaggcg
    14461 gatgttatgc aaaagttatt gatttaagtg cagaaaaaga accggaaatt tttaatgcca
    14521 ttcgcccagg tgcaatttta gaaaatacca ctttcttaga tggtacaaat gaaatagatt
    14581 ttgctagcaa aaaaattaca gaaaatacaa gggtaagcta tccgttaaac tttatatcca
    14641 atgctttaga gccaagtatt ggagggctac ctaaaaatat tttcttttta acctgcgatg
    14701 cttatggtgt tttacctcca attagtaaac taacaccagg acaagcaatg taccaattta
    14761 ttagcggtta tactgcaaaa gttgcaggta cagaagcagg tgttactgag cctaaaagta
    14821 cattcagtgc atgttttgga gcaccatttt taccattaca tccaggtttt tatgcagaaa
    14881 tgttgggtaa aaaaatgaaa gaaaataaag ttaatgtttg gctggttaat actggctgga
    14941 gtggtggtgg ttatggcgta ggtcaaagaa tgaaattaag ctatacaaga gccatgatta
    15001 cagctgcttt aaatggagag ttaaataatg tagtatttga aaaacaccca atttttggtg
    15061 tagaaatgcc aacaacctgt cctaatgttc cagaaaatat cttaaatcct aaaaatacat
    15121 ggaacgataa agaagcatac gatgctgctg ctaaaaattt agctgcacaa tttataaaaa
    15181 acttcgaaaa atatgctgct ggtgttacag aagaaatttt agcatctgct cctaaggctt
    15241 aatatttttt taataattgt atataggctg tctgttgtaa ggcagcctat atttttttaa
    15301 aaactattgc ctctaatttt ttagttttta caggttcata aattcgtata tacataatat
    15361 gcaaattatt taaggtatat aaatcacctc ctacaattac atatacaaag caagtagttg
    15421 aaataaaagt agaaaattaa aatagcataa ttgagtttgt aaagaactac tttcctaatg
    15481 cttcattagt aaaatgctca tcatgctttt ctttagcaaa tttttcttga ttaaattgtt
    15541 gagaattccc tttaggaatg gataaagatt gccaatcagc atctttaatc attttaccaa
    15601 tgtacattat ttgcccaata tgcgatgcat aatgagccaa ttgcctatta attgcatcag
    15661 taacagtatg accttgattt cttatgtaaa ctgtattatt caaatctttt tcagttaaac
    15721 tgtttaaagc cttaaataaa caatcccatc cttccttcca tttatgtaat aactcttctt
    15781 tattttttaa atcattttca aattcttcat ctctttttct ccattctttt tcgccatctg
    15841 ttgttaaaaa atctgtccat ctagaaagca tattcccaca taaatgttta acaattatgg
    15901 caatactgtt actattatta ttatactgcc aaaacaaatc tttttcttgt aactgattaa
    15961 aagttttatc tcctaaagat ttataatact caaaaagttg aatagtgttt aaaatatatt
    16021 gttgattcat aaaattattt tttgatttat caatactcta aaataaataa agttgtaaaa
    16081 tattttatta aaaatgataa atggctgtat taagtaaagt tattattaac ttcgaataat
    16141 ttttatttag tatgattgaa gtaggtaagt ttaatacatt agtaattaat agaaaggtag
    16201 attttggctt ttatttagat aatggagcag agggcattct tttaccaaaa aggtttgcac
    16261 cagcaaatgc taaaattgga gacacggtaa atgcctttat ttatcatgat agcgataata
    16321 gattaattgc tacaacccaa cagccaaatg gtgttgtagg agatattgtt ttaatgaaat
    16381 gtgtaagcac tatgtcggca ggtgcttttt tagattgggg tttaatgaaa gacttgtttg
    16441 ttgcaaaatc gcaacaaaaa gtaggcatga aagttaacgg ctggtattta gtaaaaattt
    16501 atttagatgt tcaaacaggt agagttgctg cttctgaaaa aattgaaaaa tatttagata
    16561 attcagattt aacagttgcc gaaaaagagg aagtagatat tgtaatttat agaaaaacag
    16621 atattggcta tagttgtatt attaacaatc ggcattatgg actaattcat aacaatcaag
    16681 tttttcaaaa cttagaagtt ggcgatttgc tgaaaggttt tgtacaaaca ataagagaag
    16741 ataataaaat agatattgta cttggtaaag taggctataa aaaaacagaa gacgaagtag
    16801 aaaaaataat taggctacta aaagagagta atggttattt accttatcac gataaaagtg
    16861 aaccagatga gatctataat tattttggaa tgagtaaaaa aacttttaaa atggcagttg
    16921 gtgcattgta taaacagcga aaaataaatt ttgctaaaga aggtattatt ttaagggaac
    16981 aatagactac taataattat taccaaatga aatttttgaa tttattattt gtacttttta
    17041 cagcaaattt tttatatgct caaaataaag tggttgacac caatcaggtg gtaacaccaa
    17101 acagagttaa tactgaacaa acgctacaaa agccttatgt aattttaatt tctgccgatg
    17161 gctttagata cgattatttt aaaaaatata aagccaaaaa tttattaaac attgcagcta
    17221 aaggtgtttg ggcaaaaaaa ggcatgcatc cttcttatcc atccattact ttcccaaatc
    17281 attatagcat tattacaggg ttgtatcctt cacatcatgg tatcgtagat aatatttttt
    17341 acgattctat cagaaaagaa acttataaaa ttggttctaa aacagtaatt gatggcactt
    17401 ggtataatgg cttgccacta tgggcattgg cagaaaaaca aggtatgctt tcagctagtt
    17461 tgttttgggt tggctcagag agtaatgctg ctaatacaag gccaacctat tggtatcatt
    17521 atcacgaaaa atttaaagac gatgcaaaag taaatatcat taaaaactgg ttaacattgc
    17581 cagatagtat tcggccacat ttcattactt tatattttcc tgaagtggat tattatggac
    17641 ataaatttgg cccagatgca aaggagacag aagcttctgt tcattatata gatgttgcta
    17701 taaagaaatt agttgatagt ttacaaccac ttaatttgcc tattaatttt atttttgttt
    17761 cggatcatgg aatgattgct gtagaagaga aagattatat tccactaccc gaaatagata
    17821 gtaaaaaatt tacagtaata aatagctata ccttagtgag ggttacagca aaagataaat
    17881 tagatgtact gccattatac caaaaattat taaatgaaaa aacagatgat tacaatgttt
    17941 atttagcaaa taattttcct gaaaaactaa attacagcag tagagaagat acaattggta
    18001 gaatagggga tatattatta gttcctgttg gttctaaatt attagtaaat aaaaataaaa
    18061 agccaactta tggcaaacat ggatttaata gttatgaaat tcccgaaatg aaagcaagtt
    18121 ttattgcttg gggtgaaaat tttaaaaaag gtaaaaaaat aaaaactttc caaaatattc
    18181 atatctatcc attagttgca tctattttag atttagaaat attacaacca atagatggta
    18241 gcctaaaagt gttgcaaaaa acaataagat aaaattttga agacttattg tttagtttaa
    18301 ttttttgcat atttattttt cataaatgca aaagattttt ctattaaaag cttcaacact
    18361 tttaaatcaa tatcagccag tttgttaata tataaacaag aggagcctgt tttatattta
    18421 cccaattgtt gcattaaact ttcataatca gtaaaacctg caattatata aattacaata
    18481 ttttgttttc tgggtgagaa gcctgcaaaa aaccaatccc cttctctacc actttcatat
    18541 ttatacttat attctccaaa accaataata ctgctacccc acatacttgc tttttctttg
    18601 gtaacggttt caaatagttc taataactga aaacattctt ttcttttctg ctctttttca
    18661 accaaattaa taaaatctat aaccgaagcg ttgttctttt ttgtttttaa ttcagacatg
    18721 gtattatatt ttaatcatgt tttggttctt ctaaattaaa gtaattgcct tttgttttat
    18781 ccagtacatt aataattttt gcaaaatttt cttttgtggg aagtaggtta atatacttat
    18841 ctacaatagt tctatgttta taaatgataa tagtattctt tgcttcggca tctattttat
    18901 tcaaatttac ttcagtacta gagtcgttaa aggagggcac aaaagttaat gcaatatttt
    18961 gcaaattcaa ctctttgcca aggctttcta aaaaggtaaa agtattttca tgtataaatt
    19021 ctttttcatt gcctaataca aaatatgttt tcaaatattt ttctcttaat ctactttcat
    19081 tatctaaaaa aattaaccat gtttttatct cattccaatt tgggtggtta ccaacaaaat
    19141 aaataattcc atgataacga ccatacttgc aaactgggca ggtttgcgtt cctttgtctg
    19201 caccaaaagc atgatagggt gtaaaagaag gctgatcctc gccaatgttt aaacctgaaa
    19261 ggttatttga ttgttttagt gatggataat gaggaatgtt taaacctaaa ataatatcat
    19321 gttctgcaat ttgtacttca ttgtcaatta aaactctcaa tatctcagag ccagctctat
    19381 caatcttgcc atattttttt ttatgggcta ggtataattt atcatcatca aaatataaat
    19441 ctgcaaaata ttcatttgtt atattaggct ctttaattga taaatgaata tgctgaggta
    19501 tatcttcatt tgggtaactt gctggccttg atgttttaat tgtatatttt ccgctagagt
    19561 cagttttgat ccagcctctt aatttgccat gtgcttttac tttctctgaa gtttgttcat
    19621 tgcttgaata caagccagca tcatctgtat gccaataata aattaaagta ttggcagctg
    19681 gggttttacc atcaagttga aatactgtac ctgtaataat taatttttgt ttgccctcaa
    19741 accatcccaa gctggtatgt tcattgctaa tttcatctgg tatttctaca tacattaact
    19801 cacaaccttc gcaaccacca ccaactgtat tgctattttc tttactaact tctaaaactg
    19861 attgaccatt acagcttaca aatagaataa ctagaaatac agtaaagagt ttcatacaat
    19921 tcattttata tttgtttggg aactttgtaa tcgtattatt agttgagcta ttagtaaaaa
    19981 tagttaaact aactaaatac ataaagtaat ttaatattaa atttcatttc tacgataaag
    20041 tttgagttat acatttttct gcaattcatt cagttaaaac tataattaaa ttgctgttgc
    20101 ttctacaaaa ttttagtgaa atagaaaaag aactgttttt aaaaaatgaa agcgaaatgc
    20161 aggataattt tctatctatg ttacattatt tttcaaaagc taatacaaat aatgattttc
    20221 cattactaag aagtggcttg gttttaagtg gagtaaataa ttactgaggg aaagatacta
    20281 ttttagaaaa caaagaagat ggcattgtta ctgcacagga aataagcaac ctcaatttat
    20341 taaataccga tttagtggta ttgagtgcat gccaaacagc tttaggagat attaatggaa
    20401 gcgaaggtgt gtatggtttg tagcgtgcat ttaaaatggc tggggtaaat aaattactct
    20461 tgagcttatg ggaagttcct gatactgaaa ctgcagaatt gatgcaactt ttttatagta
    20521 atttttttaa aggagattct tattattcag cttttagaaa agcacaatta acattaaaag
    20581 ccaaatacaa agacccaact aaatgggctg gatttgtatt agtaggagag taatattgtc
    20641 aaagaaagtt ttaaaatcta aaaagtcagt tttttaatta aatccaaact tcatttgtta
    20701 catatctttt gagccgttaa ttttgcacat ggcaacaata aaaccttcac tttcaaaaaa
    20761 taaagttaat cgtgttttta ttgatggttt aacttttgat gatgtacttt tattacctgc
    20821 atatagccag gttttaccaa gagaagttaa tttacattct caccttacta aagaaatagt
    20881 attaaatatt cctttgcttt cggcagcaat ggatacagtt acagaagcta atcttgctat
    20941 tgctttagca agagaaggag gtttgggcat tctgcacaaa aatatgagca ttgaaaaaca
    21001 ggcagaacaa gttagaaaag ttaaaagaag tgaaagtgga atgattatag acccattaac
    21061 cttaacaacc aattctacta ttggcgatgc tttgaagtta atgaaagaaa ataaaattgg
    21121 aggaattcca attatagata atcaaggtaa acttgttgga attcttacta acagagacct
    21181 tcgttttgaa acagataaaa aaagaaaagt taccgatgta atgactaacg aaaagttaat
    21241 tacagccccc gaaggaacag atttaaaaaa ggcagaaata attttaagaa aatataaaat
    21301 tgaaaaacta ccagtagtaa acaaacaagg caaattagtt gggctaatta cgtatcgaga
    21361 tattttgcaa cttaccaatc accctaatgc agtaaaagat aactatggtc gtttattggt
    21421 tggtgcagca ttaggcatca caaacgattt aatgcaacgt gccgatgctt taatgaatgt
    21481 aggagttgat attgttacct tagacagtgc tcatgggcac agcaaaggag taatagaagc
    21541 cttaaaagca ttaagaaaaa attttaaaaa actacaaatt attgcaggaa atgtaggcac
    21601 agcagaagga gctttggctt tggcaaatgc aggtgcagat tgtgtaaaag taggtattgg
    21661 accaggtagt atttgcacaa ctcgtatagt tgcaggtgca ggggttccac aacttacagc
    21721 agttatggaa gcaagtaatg ctttgaagaa aaaaaatatt cctgttattg cagatggtgg
    21781 tattcgttat acaggagata tggtaaaagc tttagctgct ggtgcaaata ctgtaatgat
    21841 gggcagtgta tttgctggta cagaagaaag ccctggcgaa actataattt atgaaggtag
    21901 aaagttcaaa acatatagag gcatgggaag tttaggagct atgagtcaag gcagtggcga
    21961 tagatatttt caagatgttg aagatgatgt gaaaaaattt gtaccagaag gcattgaagg
    22021 tagagttgct tttaaaggca acttaagcga aatagtgtat caatttgtag gagggctacg
    22081 ctctggtatg ggttattgtg gagcaaaaaa tattgcagcc ttgcaaaatg ctacatttgt
    22141 aaaaattact aatgcaggaa tgaaagaaag ccatgctcac gatattgata ttaccaaaga
    22201 agctcctaat tatagcagaa agtaaatcag aaattatcat ttataaacgc aaataatcca
    22261 atttaaagca tcacttaaaa gcatatttat ttgtttatag tacaaagttc ataatttatc
    22321 cttcttaatg ccattcattt gttacttttg cacccataaa aattttagtt gtttatagta
    22381 tgataaatat tagttttcct gatggtgctg taaggcaata cgaagctgga gtaagtgcaa
    22441 tggatattgc aaaaagtatt agcgaagggt tggcaaaaaa agtattggta gctggtgtaa
    22501 ataatgaggt gtgggatgca tcaagaccta ttacgacaga tagttcttta aaacttttta
    22561 cttggaacga tgccgaaggg aaaaatacat tctggcactc ttctgcacac cttatggcag
    22621 aagcaataga agcaaaattt cctggcgtta aattttgggt tggtccagct ttagaaaatg
    22681 gattttatta tgatattgat ttagacgata gaaaaattac agaagaagat ttagtggagt
    22741 tggaaaaaat aatgaacgaa ttagcaaaaa aaaataatgt atatctaaga acagaaaaag
    22801 ccaaacaaga ggcgatagct tactttacag aaaaaggcga tgaatacaaa ttagaccttc
    22861 ttcaaaactt agaagatgga actataactt tttatacaca aggtaatttt actgacttat
    22921 gtcgtggccc acatattcct aatacaggtt ttattaaagc aataaaacta ttaaatattg
    22981 caggagctta ttggaaagga gatgaaaaaa ataatcagct tacccgaatt tatggtatta
    23041 cttttcctac acaaaaagag ttagatgaat atctaacaat gttagaagaa gccaaaaaaa
    23101 gagatcatag aaaattaggt aaagaactag gaatttatac tatgaatgat gacattggcc
    23161 aagggttaat tttatggatg cccaatggaa ctattattat tgaagaatta gaaaaattag
    23221 caaaacaatc tgaagaggca gcaggctata aaagagttgt tacaccacat attgcaaaag
    23281 aaaatttgta cttaacaagc ggtcatttgc cctattatgc tgatagtatg tttgccccaa
    23341 tggaaatgga tggtgaaaaa tattatttaa gagcaatgaa ctgcccacac caccataaaa
    23401 tatttgatgc agagcctaag agttatagag atatgcctta tcgtattgca gaatatggta
    23461 cttgttacag atacgagcaa agtggtgaat tatttggatt aatgagggtt agatgcctgc
    23521 acatgaatga tgcccacatt tattgcacta aagaacaatt tgcacaagag tttaaggcag
    23581 taaacgattt gtacctaaaa tatttcaaaa tttttggtat tgaaaaatat gtaatgaggt
    23641 taagcttaca cgatcctgaa aaacttggac agaaatatgt aaacgagcca gaactttggc
    23701 ttgaaacaga agaattggta agaaatgttt taaaagaaac aggtgtacct tttgttgaag
    23761 taaaaggaga aggtgcattt tatggcccta aaatagatgt tcaaatttgg agtgccattg
    23821 gtagagagtt taccttagca actaaccagg ttgactttgc ccaacctcgt agttttaagt
    23881 tatcttttac caaccaaaat aacgaaccag aaattccttt aattattcat agagcacctt
    23941 taggcacaca tgaaagattt attggctttt tgttagaaca ttatgctggt aaatttcctg
    24001 tttggttagc accattacaa gtaaaaattt tacctatcag cgataagttt ttaccttatg
    24061 ccacaacagt taaaaatgca ttgcaacaag taggtgtaag agtagaaata gacgatagaa
    24121 atgagaaaat aggtaaaaaa attagggaag cag
//
LOCUS       DAOQPK010000007        22932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_1066_length_22932_cov_5.007650, whole genome shotgun sequence.
ACCESSION   DAOQPK010000007 DAOQPK010000000
VERSION     DAOQPK010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 22932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22932
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1066_length_22932_cov_5.007650"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PLU36_00750"
     CDS             <1..732
                     /locus_tag="PLU36_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HMZ45308.1"
                     /translation="LETTGINISSDRIIEIAVVKILPDGTKQVKRKLINPEMPISAGA
                     TQVHGITDEMVKDAPTFKQVANEVKQFMEGCDIGGYNSNRFDMPLLIEEFLRVGINFV
                     IQGRKFVDVQKVFHLMEQRTLTAAYKFYCDKNLEDAHSAEADATATWEILLAQVERYE
                     QLGTTIESIVKFTGEDDIIDFARRFIRVKGVEVFNFGKHKGKPVEQVIKEEPQYYDWM
                     MKGDFAMDTKQKFTEILNRTLLKKG"
     gene            826..1569
                     /locus_tag="PLU36_00755"
     CDS             826..1569
                     /locus_tag="PLU36_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HMZ45309.1"
                     /translation="MEKIVIIPTYNEKENIANILQAIFSLNQQFHVLVIDDGSPDGTA
                     QIVKDLFAKYPNQLFIEERTGKLGLGTAYIHGFKWCLKNGYEFIFEMDADFSHNPKDL
                     INLYNACKEGNADVAIGSRYVPGGKIENWPLDRHIYSRGGAIYTKLITWMPVNDPTAG
                     FVCYKRKVLETINLNTINFVGYAFQIEMKFAAWKLGFKIKEVPIVFIDRKEGVSKMSK
                     GIIKEGVFGVLSMQWQSILGSYNKLKRRK"
     gene            1628..2497
                     /locus_tag="PLU36_00760"
     CDS             1628..2497
                     /locus_tag="PLU36_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMZ45310.1"
                     /translation="MKAALIKLHIAVFLWGFTGVLGKAIHLNEGLLVWYRLIITIFTL
                     AVFFIFSNKNIKVPKQSIVHYAAIGVIIALHWVCFYGSIKYANVSIALVCLSSAGVMT
                     AFLEPIMTGKKIVINEVVLGLVAVAGIYLIFHFDVQYRTGIMLGVLATLFSVIFSILN
                     KKAILKVEPKTLMFYQLIGGFVVLSLLLPAYLNYFPVKTILPSLSDLVWLIVLSLVCT
                     ILAMDLSLQALKKISAFTQNLTLNLEPVYGIILAFVVYQENKELGSSFYIGFILIFST
                     VIYQMYSLLKKRG"
     gene            complement(2509..3801)
                     /locus_tag="PLU36_00765"
     CDS             complement(2509..3801)
                     /locus_tag="PLU36_00765"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMZ45311.1"
                     /translation="MRNFFNLLLILITSSTYAQQISLPDAINIALKNNFDIQIAKNTV
                     TINSINNHIGIAGGLPTVSATISDQESMIGINQKLNTGVTINRSGATSNNLTANITGT
                     MLLYNGNRVIATKNKLAELQKQSEQLLNAKIQNTIADVMVKYYDIVKQQSYIKTLELA
                     IELSRKQLELVEAQKAVGMANNADLFQTEIALNTRIQDLKLQQLIINQAKTDFLNLLN
                     LNPDSLIVINDTIINDQHILLDDVLNGIHQNPLIISLEQQIKINQLVEKETAALSIPS
                     VRTNAGFNYGRNQASAGQLLLNQSYGPFINIGVSIPIYNGSSFKRQKQVATINTNNAK
                     LQKESSVAGFKSNTIKTYQAYAANLEMASTQQNTYNISAQLVNLVLQRYQLSQATILE
                     LQEALRSFESAGYRLTNLLYTIKLSEIELKRISGKLGL"
     gene            complement(3828..6899)
                     /locus_tag="PLU36_00770"
     CDS             complement(3828..6899)
                     /locus_tag="PLU36_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HMZ45312.1"
                     /translation="MNISELSLRRPVLATVMNILIVLFGAVGYSFLSVREYPAIDPPM
                     INVSTSYAGANSDIIESQITEPIEKAVNGIPGIRSISSSSQVGRSSISIEFNVNENLE
                     AAANDVRDKVGQAIRNLPEDIDAPPVVTKSDANSDFIIILALQSHTKGLLELSDYAEN
                     VLLEKLQTIPEVSSINIFGQKKPSMRIWNNPDKMNAYNVSFKDIRNALYSENVEIPSG
                     KIYGSNTELTIRTLGRLTTEKDFKDLIIREDENGIVRLGDVAKVEIAPERDEYSWKLN
                     GLPAVGLAIIPQPGANYIKIADEFYKRIDNIKKSMKGDFELVTLFDSTKNVRKSIQEV
                     EETLLISFSLVVLVIFFFFRNILISIRPLIDIPISLIATFFIMYLAGFSVNVLTLLGI
                     VLATGLVVDDGIVVTENIFRKLEQGLPIKKAALEGSKEIFFAVISTSLTLAVVFLPVI
                     FLQGFVGSLFREFGIVVAAAVLISAFVSLTITPVLNVVLNRKKAGHGWFYDKTEPFFK
                     GMENGYKNLLQGFLKVRWMAWVIVAVCVGSTIFIYSNLQSELAPLEDKSSVRFSLIGP
                     EGASYDYMLKAGDQLSDILIDSVKEKEFAFISVPGWGGSGVNSGVARIKFVEPEFREK
                     SQSQLAKEITQKLAKINTIRVFPIEEQTISVGLGSRGSLPVQFILQNLDFEKIKEVIP
                     KFLEEARKDKTFQNVDVNLKFNKPELHLTIDRLKAKDLGLSTTDIAEVIQSAFSGRRL
                     AYYIMNGKQYEVISQVEYSDRQAPKDISKLYVRNNKGENIPLSAVTKMEESSSPPTLY
                     HYNRFKAATISASLAEGKTIGDGVKAMQAISDKLLDETFQTELSGPSRDFAESSSNTS
                     FAFILALVLIYLVLAAQFESFRDPFTIMITVPLALTGALLSLYLFNQTINIFSQIGMI
                     MLIGLVTKNGILIVEFANQKREFGMPKKQAVLEAAQQRLRPILMTSLATALGALPIAL
                     SLGAASTSRIPLGIVIVGGLLFSLILTLFVIPAVYTFISGKHAAKQTNITE"
     gene            complement(6940..7995)
                     /locus_tag="PLU36_00775"
     CDS             complement(6940..7995)
                     /locus_tag="PLU36_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ45313.1"
                     /translation="MRINLLIIIVISAFASCKENKQAEQNKGKAAQNTIVDVLLAGTK
                     SIQNVIEVNGTVIANETVTLHPEISGRLVYLNVPDGKQVAAGTLLAKINDAELQAQFN
                     KSKVQLELAQKTEERLRKLLQINGINQADYDIALNNVNNIKADIELVKAQLDKTLIKA
                     PFSGVLGLRQTSPGAYVTPQTTIATLQETNKIKIDFSVPEQFASLIKKGNTILFTTTE
                     NKVQQKATVIATEPEINTDTRNLKVRAIPNNSNINPGAFVKVFLKEGAVSNNIVIPTS
                     SIIPEASAKKVIVVKNGKGKFVEIVTGNRTATGVEVLSGLTVGDSVVVTGVLFVRPNA
                     DVKVRSVKTLDQLFNEN"
     gene            complement(8195..9031)
                     /locus_tag="PLU36_00780"
     CDS             complement(8195..9031)
                     /locus_tag="PLU36_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HMZ45314.1"
                     /translation="MQTILVPVDFSSTSYNAAKYAIELAAQLNASKIILYNAYELPVM
                     ANGGLTAPIMIDLDELKENSTKALQKMQEKLLAENKHPSLQIETLSDYNTVMNGVKHA
                     VKTLDAKLIIMGITGSSAIEQSLIGSNTIDVAKEANVPVIIIPPNAVFRSIQNTFLAC
                     SLQGLNENLPHTNIVDFLQKSKSNFGIVHIEKDLNKKYNDQDFLNTTVDSLFKMFNTK
                     HQVVYNPDFVEGINHFIDNNKVDLLIVIPKKHGFFDRLFNKSHTKMLAFHSHVPLMVI
                     HD"
     gene            complement(9128..9637)
                     /locus_tag="PLU36_00785"
     CDS             complement(9128..9637)
                     /locus_tag="PLU36_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HMZ45315.1"
                     /translation="MKIILTSINLNFMHWRKFNGESIQLPIKDAVADTIKKEFENGVK
                     LKVCIGTDSQVKGAETEFATVIVFLREHNGGFMYIHNEKTKLQYHIKERMLLEVAKSI
                     EIAYELCDLFIEFGVEMEVHADINTNPNFKSNDALKEAMGYILGMGFAFKAKPEAFAS
                     SCCADKICN"
     gene            9765..11264
                     /locus_tag="PLU36_00790"
     CDS             9765..11264
                     /locus_tag="PLU36_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45316.1"
                     /translation="MYKAEKNTIQILLLCIVLIGVYYYRPTNLYFLGDDFFHIPESVK
                     NIWIQQNSVRPIGNISLLIDYFFSNSNALGYHITNLLIHTLNSFLVAVLSKKLFNYFL
                     ILPSTNWLTWIVGIFFFVYPFHSEAVFWIIGRSGSLGALFFVAALICFLLRERSLLYS
                     IASILFFQLAIFSYESSWLFPLFICLIYFFKQGLKKILRSDIIYFALVWLLLLINLFL
                     ISKATLNLFSNYEASAFIHFNIKLLFQNFIKLFARTLLPPFFSNLYFIVALIVTFLIC
                     VLLIILFFKKKANTKFFYLLSVLWLLSYLPYLSIGIDTHGTEGERYLYLPSVFFCLWL
                     VYILYSISNTRHFFISIILLMSFQLYFLQKSRAYYVKASDITKTTINQIKLLESKEKL
                     YFDNLPQYNKGAVVFRRGLYNAIRWLYPNQKYDVVVVSIDDSDIKPRDKHINVFEVLY
                     SDKNFPKAVTAIKEWNGIEQNKIRGGGIIFNPKKDAWFSFNEWSLTIVR"
     gene            complement(11634..11825)
                     /locus_tag="PLU36_00795"
     CDS             complement(11634..11825)
                     /locus_tag="PLU36_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45317.1"
                     /translation="MKTKFLRVIGLLAIVMLIAMPVIAQDNFPDPEDDIPIDGGLTLL
                     LAAGAGYGAKKLRDKKRKM"
     gene            complement(11974..17103)
                     /locus_tag="PLU36_00800"
     CDS             complement(11974..17103)
                     /locus_tag="PLU36_00800"
                     /inference="COORDINATES: protein motif:HMM:NF019236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HMZ45318.1"
                     /translation="MATKSLPKKLIGIFCLTILLFTVVNKVQSQTTYYVNDGSLTGDI
                     ATTAIGDDLNAGTNTAPFATITKALTLAVSGDLIIADAGTYEENGFTITAGVKLSGVC
                     ASKSIIVVKVSSITLGSASELTRFTITRQSGSDVPSVALATTTGGADMKINNCFFTKC
                     RTAIYINSTTAANALTVSNNDFDDNRTGFVLADATYSNITISNNNIVDNRSFGIIFTA
                     ATTLSNISIANNNITGNLAAGIGIEGSATSGITLTNNWHGTPTVGIYLGVLNGGFSVD
                     AHNVPSSYPYNFTDLNGVAEPNYPKEIGGVGVTAISGATYASSEISFALPSCGVSSDN
                     PVGWYSTIQAAVTASNSGANIFVTSGTYAENVTVNKSLTIVGEDSALVKLDKGDANYT
                     ALGGNGFTITANNVSISKFKIRNYNNGVSNTIDASNISLSQITAIENYGSGFYSNKSI
                     NGLSITNSTFKNNGYKNNIHSGGTRRGIMTQSSSSDIANVTITNNVVTDNSLVGIDIN
                     LNRSTDGINISDNTLARNGDDELGVWLGNNNKDTSAAVVIKNNNITLSNAVRFGIEIK
                     NPSGSGDSTGVGSIFVSSNTITTSAHIGATRDMGGIVVIRRKDGYSGALCINDQPQGV
                     VIYNNTVNVENIHVNANDAYGIVVGGTGHIVYGNTINNTEVSIQLQTQNDNYLTNNSS
                     NQALTNYYFSRDNSKYSCAIVSSNTINMSGAPRLVTDVSTSTATLSGIQNINTGEYFC
                     AIQSAIDFASTNDVINVASGTYTEDLYLDKKLTIRGANFGISPSGPRMPETTLIPKSS
                     DPDPNSPTAVTMLYITATGSGSTIDGFTFDGNNPDSTSGVLINEGSVDVDACEAISSY
                     DGISNVSISNNIIQNINYCGIDFYNYTNGGTATSGNLIANNLFQGIRPSEYGMAILLY
                     NNCYTSITNNTILGVRVGVQTGNFSQTDPGTSHSITGNNIESVARGIWHNLHYNAASK
                     FDISNNNISTHVTGTANRGIMISTLQQTVGVTLSNNSISGAKYGYYLYSNPTTDTVTI
                     TGGFISSTNGIVADNYLGYNANAESSNYKLDGVSILSNDTAIIIRDDAANTNNATVSM
                     VLKNNIYANSMPSNIALAILGSDASALVTNAGGVTALHLLGNIVNNSGNSLDATNADL
                     EINGSTANISGGATVRNLISNTTTSTVIASGIGNTINITNSLTPGDGPLTTNNNLVLK
                     STATGTASILQGDVAGTYIVGDVTVEKYIPAKASRKWLFVTSPITGTLSNTWQQQIHL
                     TGAGTGGTVCPTLTAHTNGYDPTISSASNIYTYNASATAGMRWQKPATTNADNLTAGK
                     GFRLNVRGNRSLGCDLINGNPAFLAPTATTLSATGNINLSNKNAGDFTINYSNTDPLN
                     YVFIGNPYPSAISFSALYISNMANINNTYAIYIPSNAAGVYSYWDGSTFTGGSGYDNL
                     TGNIIANGQAIFVQAIGSGDLNLNFSESHKTDNTTAGYFRPNSNTQSFKVNYLNDNNN
                     KIDEIVVRFANDATISNNTIGKLDIPSMNHDTYITSLKGATHTSVQTRDINTISNDEV
                     WLNIGATKSGNYKLNFSDYDQLIGTDIYLIDHLANITQDVKQNPEYVFSVDINNAATK
                     GSSRFSVVFSQRVQPVVTQSIKMYPNPARNKVTIMLPTSADNNQHYSINVTDITGKTV
                     KVYKAVKGTQQLNIDKLTTGSYLVEITDSKGSRITEKLIKQ"
     gene            complement(17287..19755)
                     /locus_tag="PLU36_00805"
     CDS             complement(17287..19755)
                     /locus_tag="PLU36_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HMZ45319.1"
                     /translation="MKKIFLFFSLFCYTVLLAQHSGSQKTKDDSWKVQYRAFPEKINN
                     LVHTKLVAKFDYSKSYLLGKAWITLQPHFYATDSLALDAKGMEIHKLDIIKNGKSEKL
                     KYNYDGMVLKINLDKTYKRTEKYTVYIEYTAKPNELKVEGSSAITDAKGLYFINPEGK
                     EKNKPTQVWTQGETEATSVWVPTIDRPNQKTTQEIYMIVPNKYVTLSNGKLVSQVKNT
                     DGTRTDYWKMDKPHAPYLFFMGIGDYAVVKDNYKGKELSYYVEHEYEKVAKKIFGNTP
                     EMMQYFSKILGVEYPWNKYAQIVGRDYVSGAMENTTATLHQESAQQDARQLVDENHWE
                     GTIAHELFHHWFGDYVTAESWSNLTVNESFANYSQTLWAEYKYGKDAGDEENYNDMQG
                     YLMSGSSKKDLVRYFYKDKEDMFDAVSYNKGGRILHMLRNIVGDDAFFKSLNKYLTNN
                     KFGTGNADKLRLAFEEVTGKDLNWYWNQWFYGSGHPQLDISYAYNEDNKKATVYIKQT
                     QSGDKIFTLPFSIDVYLGKEKNRYEFLLNSREDSVSFSVSSKPDLINVDGDKILLCNK
                     KDNKTLKEYIYQYKNAGKYLDRREAIDFASKQIKNAESIVFLEEVLNDTYAPFRIKAI
                     QTLAKTTVPENIIQRIEEMAIKDKNSKVRSSAIDFLAKLENKKYRSIFEKSVYDSSYS
                     IAGASLEALEAIDSEAALAITKKIMKEPAKGRLSAAISNVLVKHGGDEAFDFVAENFE
                     KAPFSQAKFETMQALADILVKVTDFKKFIKGVDLIVEFRDSIPEAYKAQITAYINNMV
                     LKTIADKKEAAGDKEKADYVKSKL"
     gene            19909..21099
                     /locus_tag="PLU36_00810"
     CDS             19909..21099
                     /locus_tag="PLU36_00810"
                     /inference="COORDINATES: protein motif:HMM:NF026121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4476 domain-containing protein"
                     /protein_id="HMZ45320.1"
                     /translation="MKYFKLKTLICLVIVLLAFSDANAQKKHFIFVQSENNEPFYIMI
                     NNKNYSSTLSGYLIIPRLKNGKYFFVTGFPKEKYPEQKFSYIINDKDVGFVLKQNGKS
                     GWSLFNVVDFTNIMANAPDWETDKIKNDTIQIEDIYAINTSSKPSTHSSSEVIINSTT
                     PTTENISPKPLAHVNKDTIKFQTNTVLSTNNSTNHTAKEVESTNTTNTQTSTSKNNIS
                     VNSEKKIIKTYQKNDINGIDEMYIDYTTNPSDTIIIFIPINAQEEIQDTQEKHNTDKI
                     NYSNSPKSKNSSNTISASNSTQYNTSCVYLATEADYLKTRKIMSYETSDDKMIKAAKK
                     SFTNKCYYVEQIQKLGLLFISEQSRLKFFKEAYPNVYDKSNFASLEIQFTISSVINQF
                     RQVK"
     gene            21102..21845
                     /gene="truA"
                     /locus_tag="PLU36_00815"
     CDS             21102..21845
                     /gene="truA"
                     /locus_tag="PLU36_00815"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HMZ45321.1"
                     /translation="MPRYFIELAYCGTNYSGFQIQKNASTIQEKIEQALKTIIKQEIN
                     LTGSSRTDAGVHAHQNFFHFDIEQKVNQQIIYNLNAILPNDIAIKKIHLVTPNAHCRF
                     HATSREYQYHIYQQKNPFLQNQAWFYPYPLNINILNQAATIIASQNNFSTFSKKHTQV
                     NNFNCTIFKSQWAQNNNIITYTVEANRFLRGMVRGLVSTMLLVGINKITLQQFQNIIN
                     SHNCSNANFSAPAQGLFLINVKYPSDLFL"
     gene            22568..>22932
                     /locus_tag="PLU36_00820"
     rRNA            22568..>22932
                     /locus_tag="PLU36_00820"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         7478 a         3687 c         3381 g         8386 t
ORIGIN      
        1 ttagaaacaa ctggtataaa tataagttct gatagaatta tagaaattgc agttgtgaaa
       61 attttaccag atggaacaaa acaagtaaaa agaaaattaa ttaatcctga aatgcctata
      121 tctgctggtg caacacaggt tcatggtata acagatgaaa tggtaaaaga cgctcctact
      181 tttaaacaag tagctaatga agtaaaacaa tttatggaag gctgtgatat aggagggtac
      241 aacagtaata gatttgatat gccattatta atagaagaat ttttaagagt tggaattaat
      301 tttgtaatac aaggaaggaa gtttgtagat gtgcagaaag tttttcattt aatggaacaa
      361 agaacattaa ctgcagctta taagttttat tgtgataaga acttagagga tgctcatagt
      421 gccgaagctg atgcaacggc aacatgggaa attttacttg cacaagtaga gaggtatgaa
      481 caattgggta caacaataga aagcattgtt aagtttaccg gagaagatga tattatagat
      541 tttgcaagaa gatttattag agtaaaaggg gtagaggttt ttaactttgg caagcacaaa
      601 ggaaaacctg tagagcaagt aattaaagaa gaaccacaat attacgattg gatgatgaaa
      661 ggtgattttg caatggatac caaacaaaaa ttcactgaaa ttttaaaccg cactttgtta
      721 aaaaaaggct aagtaataga aagggtgtaa agtaattgcc tctaaattta ttttggtaaa
      781 aatgattagt tactttagtg cctttaataa aatggatacc ccagattgga aaaaattgta
      841 attataccta cttataacga aaaagaaaat attgccaata ttttacaagc tattttttct
      901 ttaaaccaac agtttcatgt gttggttata gatgatggaa gcccagatgg cactgcacaa
      961 attgtaaaag atttatttgc aaaatatcct aatcaattat ttattgaaga aagaacagga
     1021 aaacttggtt taggtacagc ttatattcat gggtttaagt ggtgcttaaa aaatgggtac
     1081 gaatttattt ttgaaatgga tgccgatttt tctcataacc caaaagattt aataaactta
     1141 tataatgcct gtaaagaagg aaatgcagat gtagccattg gtagcaggta tgtacctgga
     1201 ggtaaaattg aaaattggcc tttagatagg catatttact caagaggtgg ggcaatctat
     1261 acaaaactaa ttacatggat gcctgtaaac gacccaactg ctggttttgt atgttataaa
     1321 agaaaagtat tagaaacaat taacttaaat acaataaatt ttgtggggta tgcttttcag
     1381 atagagatga agtttgctgc atggaaatta ggttttaaaa ttaaagaagt accaattgtt
     1441 tttattgata gaaaagaagg tgtaagtaaa atgagtaaag gcattattaa agaaggtgtt
     1501 tttggtgtat taagtatgca atggcaaagc attttaggta gttataataa acttaaaaga
     1561 agaaaataat tttttactac taattgttgt attttcaact aacaattaca attatcttat
     1621 tttttaaatg aaagcagcat taataaaact acatatagct gtttttcttt gggggtttac
     1681 cggtgtgtta ggcaaagcaa ttcatttaaa tgaaggcttg cttgtttggt atagattaat
     1741 tattaccatt ttcacacttg ctgttttttt tatcttctct aataaaaata taaaagtacc
     1801 caaacaatct attgtacact atgctgccat aggtgtaatt attgcattgc actgggtttg
     1861 tttttatggt agtataaaat atgccaatgt ttctattgct ttagtttgtt tatcctctgc
     1921 aggtgtaatg actgcttttt tagagcctat tatgacaggt aaaaaaattg ttataaatga
     1981 agtagtgtta ggcttggttg cagttgcagg tatttattta atttttcatt ttgatgtaca
     2041 gtacagaaca ggaattatgt tgggtgtttt agcaacttta tttagtgtaa ttttttctat
     2101 tttaaataaa aaagctatcc ttaaagttga acctaaaacg ctaatgtttt atcagcttat
     2161 aggagggttt gtagttctgt ctttgcttct tccggcttat ttaaattatt ttcctgttaa
     2221 aactattttg ccttctttat cagatttagt ttggcttata gttttaagtt tagtttgtac
     2281 catacttgca atggatcttt cattgcaagc actcaaaaaa atatcggcat ttacacaaaa
     2341 ccttacgcta aatttagaac cagtatatgg tattatttta gcctttgttg tatatcagga
     2401 aaataaagaa ttaggcagta gcttttatat tgggtttatt ttaattttct ctacagtaat
     2461 ttatcaaatg tatagtttgc taaaaaaaag aggttaattt ttttagaatt acaaacctaa
     2521 tttaccacta attcttttta attcaatttc tgaaagtttt atggtgtaaa gtaaattagt
     2581 taaacgatag ccagcacttt caaaacttct taatgcctct tgcaactcta aaattgttgc
     2641 ttgcgataat tgataacgtt gtaacacaag atttactaat tgagccgaaa tgttataagt
     2701 attttgttgt gtacttgcca tttctaaatt tgctgcataa gcttgatatg ttttaatagt
     2761 attactttta aaacctgcta cggaactttc tttttgaagt ttagcattat tagtgtttat
     2821 agtagctact tgtttttgac gtttaaaact actgccatta tatattggaa tggaaacacc
     2881 aatattgatg aatggcccat aagattggtt taaaagtaat tgccctgcag aagcttgatt
     2941 tcttccataa ttaaaccctg cattggttct tacagaagga atgcttaatg ctgctgtttc
     3001 tttttcaact aattggttaa tttttatttg ttgctctaaa gagataatta atggattttg
     3061 atgaatacca tttagtacat catctaataa aatatgttga tcattaatta ttgtatcatt
     3121 aataacaatt aaactatctg gattaaggtt aagcaaatta agaaaatcgg ttttagcctg
     3181 gttaataatt agctgctgta atttaaggtc ttgtattctt gtatttaatg caatttcagt
     3241 ttgaaataaa tcagcattat tagccatgcc aactgctttt tgagcctcta caagttcaag
     3301 ttgttttctg cttagctcaa ttgcaagttc taaggtttta atatagcttt gttgttttac
     3361 aatatcataa tattttacca ttacatctgc aatggtattt tgaattttag catttaaaag
     3421 ttgttcactt tgtttttgta attctgctaa tttatttttt gtagcaataa ccctattgcc
     3481 attatacaaa agcattgtgc cggtaatgtt ggcagttaaa ttattactag ttgcaccact
     3541 tctatttatg gttactccgg tatttaattt ttgattaata ccaatcatac tttcctgatc
     3601 tgaaatggtt gcagatacag taggtaagcc accagcaata cctatatgat tattgatgct
     3661 gttaatagtt actgtatttt ttgctatttg aatatcgaaa ttatttttaa gtgcaatatt
     3721 aattgcatcg ggtaaactta tttgctgtgc ataagttgaa gaagtgataa gtataagtag
     3781 taagttgaaa aaatttctca tgttgctaaa acttattgta acaaaattta ttcggtaata
     3841 ttagtttgtt tggctgcatg tttaccacta ataaatgtat aaacagcagg tattacaaac
     3901 aatgtaagta taagtgaaaa caatagccca ccaacaatta caatacctaa aggaatacga
     3961 cttgtacttg ctgctcctaa gcttaatgca atgggtaatg cacctaaggc agttgcaagg
     4021 ctagtcatta aaattggtct taatctttgt tgtgctgctt ctaaaactgc ttgcttttta
     4081 ggcattccaa attctctttt ttggttagca aactcaacaa ttaaaatacc atttttagta
     4141 actaacccaa taagcataat cattccaatt tgagagaaaa tgttaatggt ttggttaaat
     4201 aaatataaac ttaataatgc ccctgttagt gctaagggta cagtaatcat aattgtaaaa
     4261 gggtctctaa aactttcgaa ctgtgctgcc aatactaagt aaattaaaac caaagcaagt
     4321 ataaaagcaa aggaggtatt gctgctactt tctgcaaagt ctctacttgg cccacttagc
     4381 tctgtttgaa aggtttcgtc taataattta tcagaaatgg cttgcattgc tttcactcca
     4441 tcgccaattg ttttaccttc tgctaacgat gcagaaatgg ttgctgcttt aaaacggttg
     4501 tagtgataaa gtgttggagg gctagaactt tcttccattt ttgtaactgc agataatgga
     4561 atattttctc ctttattatt tctaacataa agcttagaaa tatctttagg tgcttgccta
     4621 tcgctgtatt caacctgcga aattacttca tactgctttc cattcataat ataataagcc
     4681 aaccttctac cactaaatgc actttgtatt acctctgcaa tatcggttgt acttaaacct
     4741 aaatctttag cttttaatct atctatagtt aaatgtaatt cgggtttatt aaacttcagg
     4801 ttcacatcaa cattttgaaa agttttgtcc tttcttgctt cttctaaaaa ctttggtata
     4861 acctctttaa ttttttcaaa atctaaattt tgcagaatga attgcactgg taaggagccc
     4921 ctgctaccta aaccaacaga tatagtttgc tcttcaatag gaaaaactcg aatggtattt
     4981 attttggcta atttttgcgt aatttctttt gctaattggc tttgagattt ttctctaaat
     5041 tctggttcaa caaatttaat tcttgcaaca cctgagttaa caccagagcc tccccaacct
     5101 ggtacagaaa taaaagcaaa ttctttttct tttacactgt ctattaaaat atctgatagc
     5161 tgatctcctg ctttaagcat ataatcgtag ctagcacctt ctggacctat taaactaaat
     5221 cttacactac ttttatcttc tagtggtgct aattcagatt gtaaattgga gtaaatgaat
     5281 attgttgagc ctacacaaac tgctactata acccaagcca tccaacgaac ttttaaaaag
     5341 ccttgtaata ggtttttgta cccattttcc attcctttaa aaaatggttc tgttttatca
     5401 taaaaccaac catgccctgc tttttttctg tttaaaacta cgtttaatac aggtgttata
     5461 gttagcgaaa caaaagcact aattaacaca gctgctgcta ctacaatgcc aaactctcta
     5521 aacaaactgc ccacaaaacc ttgtaaaaat ataactggta aaaacaccac tgccaaggtt
     5581 aacgaagtag aaattacagc aaagaaaatt tctttactgc cttctaaagc agcttttttt
     5641 attggcaagc cttgctctaa ttttctaaaa atattttcag ttacaactat accatcatct
     5701 actactaaac ctgtggccaa tactatacct aaaagagtta gcacattaac actaaaccct
     5761 gcaaggtaca taataaagaa agtggcaatt aacgaaatag gtatatctat taaaggacga
     5821 atggaaatta atatatttct aaaaaagaag aaaataacca ataccaccaa gctaaaagat
     5881 atgagtaagg tttcttctac ttcttgaata ctttttctta cattttttgt actatcaaat
     5941 aaggttacta attcaaaatc gcctttcatc gattttttaa tattatctat ccgtttataa
     6001 aactcatcgg caatttttat atagtttgcc cctggctgtg gaataattgc taaaccaaca
     6061 gctggtaagc catttaactt ccaactatat tcatctcgtt caggtgctat ttcaactttt
     6121 gctacatcac caagccttac aattccattt tcatcttctc ttatgattaa atctttaaaa
     6181 tctttttctg ttgttaatct acctaatgtt cttatagtta actctgtatt gctaccataa
     6241 attttacccg atggaatttc tacattttcg ctgtatagtg catttctaat atctttaaaa
     6301 gaaacattgt atgcattcat tttatctgga ttattccaaa tacgcatact tggttttttt
     6361 tgcccaaaaa tattgatgct actaacttct ggtatagttt gaagtttttc tagcaataca
     6421 ttttctgcat aatcgctaag ctctaataaa cctttagtgt ggctttgcaa ggcaagtatt
     6481 attatgaaat cgctgtttgc atcgcttttt gtaactactg gtggtgcatc aatatcttct
     6541 ggtaagtttc taatagcttg ccccacttta tctcttacat catttgctgc agcttctaag
     6601 ttttcgttta cattaaactc aattgaaatg ctacttctac caacctggct tgatgaactg
     6661 atagatcgga ttccaggaat tccattaact gctttttcta ttggttcggt aatttggctt
     6721 tcaataatat cgctgttagc accagcataa gaggtactaa cgttaatcat tggtgggtca
     6781 atagctggat attctcttac agataaaaat gaatagccta cggcaccaaa taatacaatc
     6841 agaatattca ttactgttgc taatactggg cgtcttaatg atagttcgga tatattcata
     6901 attcttagaa aaatctaaaa aaatattttt aataaagtat taattttcgt tgaaaagttg
     6961 gtctaaagtt tttacacttc tcacttttac atctgcattt ggcctaacaa ataatacacc
     7021 agttacaaca accgaatctc caactgttaa accacttaaa acttctacac cggttgctgt
     7081 tctatttcct gtaactattt caacaaattt tccttttcca ttttttacta caattacttt
     7141 tttagcagaa gcttcgggta taatggaact tgtaggaata acaatattgt ttgaaacggc
     7201 accttctttt aaaaatactt ttacaaatgc accaggattt atatttgaat tatttggaat
     7261 tgctctcact tttaaatttc ttgtatctgt atttatctct ggctctgtag ctattacagt
     7321 ggctttttgt tgtactttat tttccgtagt ggtaaataaa attgtgttgc ctttttttat
     7381 taagcttgca aattgttctg gtacagaaaa atcaatttta attttgttgg tttcttgtaa
     7441 agttgcaata gttgtttgag gggtaacata ggcaccaggg cttgtttgcc ttaaacctaa
     7501 aacgccgcta aaaggtgctt taattaaagt tttatctaac tgagctttta ctagttcaat
     7561 atctgcttta atgttattta cattgtttag ggctatatca taatcggctt ggttaatgcc
     7621 atttatttgt aatagttttc ttaaacgctc ttctgttttt tgagcaagtt ctaactgcac
     7681 tttactttta ttaaattgag cttgtaattc agcatcgtta attttagcta ataatgtacc
     7741 agcagcaact tgttttccat ctggcacatt taagtaaacc aatcttccac taatttccgg
     7801 atgtagtgtt actgtttcgt ttgctattac ggtaccatta acctctatta cattttgaat
     7861 agatttagta cctgctaata acacatctac tattgtgttt tgtgctgctt ttcctttatt
     7921 ctgttctgct tgcttatttt ctttgcaaga tgcaaatgcc gatattacga tgatgattag
     7981 aagatttata cgcataggtc aaagttggaa tggctaagat aaccaaataa aatttttatt
     8041 taacgaggct tttataagtg gcgaaaatat attttaaagt tgttttactt ccaatcaata
     8101 gagatgaacg gtgaatttta ccttaagtta tacatatttt tcctttattc ataaagttaa
     8161 tggtaaatat gaagtacaaa aagtggtgtt atgtttaatc atgaataacc attaaaggta
     8221 catgactatg aaaagccaac attttagtat ggcttttatt aaacaacctg tcgaaaaagc
     8281 catgtttttt aggaatgaca attaataaat ctactttatt attatcaatg aaatgattga
     8341 taccttctac aaaatctgga ttatacacaa cttgatgctt agtattaaac attttaaata
     8401 aggaatcaac agtggtattt aaaaaatctt gatcattata cttcttattt aaatcctttt
     8461 ctatatgaac aattccaaag ttagatttag atttttgcag aaagtcaact atattagtat
     8521 gtggtaagtt ttcgtttaaa ccttgtaagc tacaagctaa aaaagtattt tgaatagatc
     8581 taaaaacagc atttgggggt ataataatta caggtacatt ggcttctttt gcaacatcta
     8641 ttgtattact gccaattaat gattgttcta ttgcagagct accagtaatg cccattataa
     8701 tgagttttgc atctaatgtt tttacagcat gtttcacacc attcataact gtattgtaat
     8761 cactaagtgt ttcaatttgt aaggagggat gtttattttc tgcaagtaat ttttcttgca
     8821 ttttttgtaa agccttagtg ctattttctt ttagttcatc taaatctatc attataggtg
     8881 ctgttaagcc accatttgcc atcactggta attcataagc attgtacaaa attattttag
     8941 aagcattgag ttgtgcagct aattctatag catattttgc agcattataa gatgtagaag
     9001 aaaaatcaac tggaactaag atggtttgca tacaaatatt tttatagtag taaagatagc
     9061 actataaaaa aataaggtaa gtaaatttat tgttgaagag attaaaacaa gaattaaact
     9121 tatttaatta gttacaaatt ttatcggcac aacaactgct tgcgaatgct tctggtttag
     9181 ctttaaaagc aaaacccatt cctaaaatat agcccattgc ctcttttaga gcatcgttac
     9241 ttttaaaatt tgggttggta tttatatctg catgtacttc catttcaaca ccaaattcaa
     9301 taaacaaatc acatagttca taagcaattt caatgctttt ggcaacctct agtaacattc
     9361 tttcttttat atgatattgc agttttgttt tttcgttatg tatatacata aagccaccat
     9421 tgtgctctct taaaaaaaca attaccgttg caaattctgt ttctgcacct ttaacctggc
     9481 tatcggtgcc aatacaaact tttaatttta caccattttc aaattctttt tttatggtat
     9541 ctgcaactgc atctttaatt ggtagctgga ttgattcgcc attaaatttt ctccaatgca
     9601 taaaatttag gtttattgaa gttaatatta ttttcatgta attatttcta agttattata
     9661 cacaaaaggt gcataaccat tgtatttatt aaaactcagg tttttatacg cccatttatt
     9721 gaaatacaat tctgttttct ttatttttaa tttagattta ttttatgtat aaagcagaaa
     9781 aaaatactat tcaaatttta ttgctttgca tagtattgat aggtgtttat tattacaggc
     9841 caactaattt atatttttta ggtgatgatt tttttcacat accagagagt gtaaagaata
     9901 tttggattca acaaaactct gtaaggccaa ttggtaatat ctctcttctt attgattatt
     9961 tttttagcaa cagtaatgcc ttgggctatc atattactaa tttacttatt cataccttaa
    10021 atagcttttt agtagctgta ctaagtaaaa aattatttaa ttatttttta attttaccct
    10081 ctacaaactg gcttacttgg atagttggaa ttttcttttt tgtatatcca tttcatagcg
    10141 aagctgtgtt ttggattatt ggcagatctg gtagtttagg agcattgttt tttgttgctg
    10201 cattaatttg ctttctctta agagagcgtt cattattata ttctattgct tctatactat
    10261 tttttcaact tgcaattttt agttacgaat cgtcttggct gtttccgtta tttatttgct
    10321 taatttattt ttttaagcaa gggcttaaaa aaatattaag aagtgatatt atttactttg
    10381 ctcttgtttg gcttttgtta ttgattaact tattcctcat ttctaaagca acacttaatc
    10441 tttttagtaa ttacgaagca tcggcattta tacattttaa tataaaacta ttatttcaaa
    10501 attttataaa actatttgct agaacattgt taccaccctt ttttagtaat ctttatttta
    10561 ttgttgcttt aattgttacc tttcttattt gtgttttatt aattatttta ttttttaaga
    10621 agaaagcgaa tactaagttc ttttatttgt taagtgtatt atggctgttg agttaccttc
    10681 catacttgtc tattggtatt gatacccatg gaacagaagg tgaaaggtat ttatatttac
    10741 catctgtatt tttttgttta tggcttgttt atatcttata cagtataagc aatacaaggc
    10801 atttttttat ttctattatt ttactaatga gctttcagct ttatttttta caaaaaagta
    10861 gagcatatta tgtaaaagca agcgatataa ctaaaacaac aataaaccag attaaattat
    10921 tagaaagcaa agaaaaattg tatttcgata atttaccaca atacaataaa ggggctgttg
    10981 tttttagaag agggctttac aacgctatta gatggttata tcctaatcaa aaatatgatg
    11041 ttgtagtagt tagtattgat gatagtgata ttaaaccaag agataagcat ataaatgttt
    11101 ttgaagtatt gtatagcgat aaaaactttc ctaaagcagt tactgccatt aaagaatgga
    11161 atggtattga acaaaataaa ataagaggtg gtggaattat ttttaatcct aaaaaagatg
    11221 catggtttag ttttaatgaa tggtctttaa ctattgtaag ataatctaat ataggaatat
    11281 gaattgccct aaattaactt ttaaatgtat tatccttcag ttctagttaa ataagtaatt
    11341 ctaaatctat cagtatttaa accattttta aaactacaat tttttattac aaattcttta
    11401 ccattatgta aactgatagg aatatctaat ctattagggt ttacatgtgt ggctactttg
    11461 ctactttcat taattataca ttatcctata attggggtaa tgttatttag cttaccactc
    11521 aagtaatctg tataaaaatc tatttgtttt aataagttta tattgatatt ataaaaaagg
    11581 tattgcattg ctgcaatacc ttttaaggag taattattat atattacaat ctcctacatt
    11641 tttctttttt tgtctcttaa ttttttagca ccatagcctg cacctgctgc tagtaataaa
    11701 gttaatcctc catcaattgg tatatcatct tcaggatctg gaaaattatc ttgtgctata
    11761 actggcattg ctattaacat tactattgct aataagccta ttactcttaa aaattttgtt
    11821 ttcatatttt tttacttttt atacttttca gaacgaaatg aagaatctgt ttgtgttctt
    11881 cctattgtca ttttgagcgt aagcgaaaca tctgcttgtg cattagtaca tttttcagca
    11941 gatccttcgc ttcgctcagg atgacattat atattattgt ttgattaatt tttctgttat
    12001 tctgctgcct ttgctatctg ttatttccac taaataacta ccagttgtta acttatctat
    12061 gtttaattgt tgtgtacctt tcactgcctt atataccttt actgttttac ctgtaatatc
    12121 agttacgttt atgctataat gttggttgtt atcagcactt gtaggtaaca ttatggtaac
    12181 tttatttctt gcagggttag gatacatttt aatgctttgg gttaccacag gttgtaccct
    12241 ttggctaaat actacactaa acctgctact tcctttagta gctgcattat taatatctac
    12301 actaaataca tactctgggt tttgttttac atcttgtgta atattggcta aatggtctat
    12361 taagtatata tctgttccaa ttagttggtc ataatcgcta aagtttaact tgtagttgcc
    12421 gcttttggtt gcacctatat ttaaccaaac ctcatcgttg ctaatagtat taatgtctct
    12481 tgtttgtact gatgtatgtg ttgcaccttt taagcttgtt atataagtgt catggttcat
    12541 acttggtata tctaatttac caatggtatt attactgatg gttgcatcat ttgcaaaacg
    12601 aaccactatt tcatctattt tattattatt atcatttaaa taatttactt taaaactttg
    12661 tgtatttgaa ttaggtcgaa aatatccagc agtagtatta tctgttttat gtgattcaga
    12721 aaagtttagg tttaaatcac cagacccaat tgcttgtaca aatattgcct gaccattggc
    12781 aataatattg cctgttaaat tatcgtatcc agaacctcca gtaaatgtac taccatccca
    12841 atagctatat acaccagcag cattggatgg tatatatatg gcataggtat tatttatatt
    12901 ggccatattg ctaatataca atgcactaaa gctaatagca ctaggataag ggttgccaat
    12961 aaatacataa ttaagaggat cggtattgga gtaattgatt gtaaaatctc ctgcattttt
    13021 attgcttaaa ttaatattac cagttgcact taaagtagtg gcagttggtg ctaaaaatgc
    13081 tggattacca tttattaaat cgcagcctaa gcttctgttg ccacgtacat ttaatctaaa
    13141 gccttttcct gcagttaagt tatcggcatt ggtggttgca ggtttttgcc aacgcatacc
    13201 agcagttgca gaggcattgt aggtataaat attagatgca ctactaatag taggatcata
    13261 accattagta tgtgcagtaa gtgtagggca aacagttcct cctgtgccag cacctgttaa
    13321 atgtatttgt tgctgccatg tattacttaa tgtgccagtt attgggcttg taacaaatag
    13381 ccattttcta cttgctttgg ctggtatata tttttctact gttacatcac ctactatata
    13441 ggtgcccgct acatcgcctt gcaaaataga agctgtacca gttgctgttg attttaatac
    13501 taaattatta ttggtggtaa gtgggccatc acctggtgtt aaggaattag taatattaat
    13561 cgtatttcca atacctgaag ctattacagt acttgtagtt gtattgctaa tcaagtttct
    13621 aacagttgca ccaccagaga tgtttgctgt tgaaccattt atttctaaat ctgcattggt
    13681 tgcatctaac gaatttccac tattatttac aatgtttcct aataaatgta aggcagtaac
    13741 ccctcctgca tttgttacta atgcggaggc atcgcttcct aatattgcta atgcaatatt
    13801 ggacggcatg ctattagcat aaatattatt tttcaatacc atagaaacag ttgcattatt
    13861 agtgtttgcc gcatcgtctc ttattattat tgcagtgtca tttgataaaa tactaacacc
    13921 atcaagtttg tagttacttg attctgcatt tgcattatat cctaaataat tatctgcgac
    13981 aattccattt gtagaagaga taaaacctcc tgttatagta actgtatctg ttgtagggtt
    14041 actatacaaa taatatccat actttgctcc acttatagaa ttattactaa gtgtaactcc
    14101 aacggtttgc tgtaatgtag aaatcataat acccctattg gctgtacctg taacatgtgt
    14161 tgatatgtta ttgtttgaga tgtcaaattt ggaagctgca ttataatgta gattatgcca
    14221 aatacctctg gcaacgcttt caatattgtt tcctgtaata gaatgactgg ttccagggtc
    14281 cgtctgagaa aaattacctg tttgaacgcc aactcttaca cccaatatag tattgtttgt
    14341 tatacttgta tagcagttgt tataaagtaa aattgccatt ccgtattctg aaggtctaat
    14401 accttgaaat aaattattag ctattaaatt tcctgatgtt gcagtaccac cattagtata
    14461 gttataaaaa tcaattccgc aatagttgat attttgtata atgttattag aaatactaac
    14521 attcgaaatt ccgtcgtatg atgaaatcgc ttcacaagca tctacatcta cacttccttc
    14581 attaataaga acaccactag tgctatccgg attatttcca tcaaaagtaa atccatcaat
    14641 tgtagagcca ctaccagttg ctgtaatata tagcatagta actgcagtag gactatttgg
    14701 atcaggatct gaacttttag gaattaaagt agtttctggc attcttgggc cagaaggact
    14761 tattccaaaa tttgcacccc taatggttag tttcttatct aaatataaat cttcggtata
    14821 agttccactt gccacattta taacatcatt ggtagatgca aagtcaatag cagattgaat
    14881 agcacaaaaa tattcgcctg tatttatatt ttgtattccg gataatgttg ctgtacttgt
    14941 agaaacatct gttaccaacc ttggagcacc agacatatta attgtattgc ttgaaactat
    15001 agcacaagaa tattttgaat tatctctact aaaatagtag ttggttaatg cttgattgga
    15061 actattgttt gttaaataat tatcgttttg ggtttgaagt tgtatcgaga cttctgtatt
    15121 gttaattgta tttccatata caatatgacc tgttcctcca actactatac cataagcgtc
    15181 gtttgcattt acatgtatgt tttctacatt aacggtatta ttataaataa ctactccttg
    15241 tggttggtca ttaatacata gtgcaccaga ataaccatct tttcttctaa ttactactat
    15301 acctcccata tctctagttg caccaatatg tgcacttgtt gtaatagtgt tgctagaaac
    15361 aaaaatactt cctacacctg tagagtctcc actaccagat gggtttttaa tttcaatgcc
    15421 aaaccttaca gcattactta gtgttatatt gttattttta ataaccactg cagcactggt
    15481 atctttattg ttattaccca accatacacc aagttcatca tctccatttc ttgctaaagt
    15541 attatcagag atgttgatac catctgtaga tctgtttaag ttaatatcta taccaaccaa
    15601 gctattgtct gtaactacat tattggtaat agtcacatta gctatatcgc tactgctaga
    15661 ttgtgtcatt atacctcttc ttgtacctcc actatgaata ttatttttat agccattgtt
    15721 tttaaaggtt gaattggtaa tacttaatcc attaatagat ttattactgt aaaaaccaga
    15781 tccatagttt tcaattgcag taatttgaga taaagaaata ttacttgcat ctatagtatt
    15841 agaaactcca ttattgtagt tgcgaatttt aaatttagaa atagaaacat tatttgcagt
    15901 aatagtaaac ccattaccac ctaatgcagt atagttagca tcgcctttat ctagttttac
    15961 taaagcacta tcttctccaa caatagttaa agatttattt acagtaacat tctccgcata
    16021 agtaccagat gttacaaaaa tatttgctcc ggagttagag gctgtaactg ctgcttgtat
    16081 agttgaatac caaccaactg gattatctga acttactcca caagaaggta atgcaaagct
    16141 aatttcagat gatgcatacg tagcaccaga tattgcagtt acaccaacac caccaatttc
    16201 ttttggataa tttggttcgg caaccccatt taaatctgta aaattatatg gatatgaaga
    16261 aggaacatta tgagcatcta cactaaagcc accatttaaa acacctaggt aaataccaac
    16321 tgttggtgtt ccgtgccaat tgtttgtaag tgtaatacca ctagttgcac tgccttctat
    16381 accaattcct gcagctaaat ttcctgtaat attattattt gcaatagaaa tatttgaaag
    16441 ggtagtggca gcggtaaaaa ttattccaaa acttctgtta tctacaatat tattattaga
    16501 tatagtaata ttagaataag tagcatctgc taaaacaaaa ccagttctat tatcatcaaa
    16561 atcgttattg cttacagtta aagcatttgc tgcggttgta gagtttatat aaattgcagt
    16621 tctgcatttt gtaaaaaagc aattatttat tttcatatct gcaccaccag ttgttgtagc
    16681 taatgcaaca cttggtacat cacttcctga ttgccttgtg atggtaaaac gtgttagttc
    16741 tgaggcggag cctaatgtaa ttgaactaac tttaactact ataatacttt tagatgcaca
    16801 tacaccactt agttttacac ctgctgtaat ggtaaaacca ttttcctcgt aggtgccagc
    16861 atctgcaatt attaaatcac cactaacagc tagggttagt gcttttgtaa tggtggcaaa
    16921 aggtgcagta ttggtgcctg catttaagtc atcgccaatt gcagttgttg caatatctcc
    16981 tgttaaggaa ccatcattta cataataagt tgtttggctt tgtaccttat ttacaaccgt
    17041 aaatagtaaa atggttaaac aaaaaatacc aattaatttt ttgggtaaag atttagttgc
    17101 cataaattgt agttttaaat aaataatttt tttttaaaaa aacagtttag gatactcgat
    17161 tttaagttga taagttttaa ggggggaact caaatatata aacttttaat aattaaaaaa
    17221 aataaataat aaaaaagcta ccacttactg tgatagcttg agtaataaaa taattaagaa
    17281 ttaaccttac aatttgcttt ttacgtaatc tgccttttct ttatcacctg ccgcttcttt
    17341 tttatctgca atggttttaa gaaccatatt gttaatatat gcagtaattt gtgctttgta
    17401 ggcttctggt attgagtctc taaactcaac aattaaatca acgcctttta taaatttttt
    17461 aaaatctgtt actttaacca aaatatcagc taaagcctgc atggtttcaa atttagcttg
    17521 agaaaagggg gctttttcaa aattttcagc tacaaaatca aatgcttcat cgccaccatg
    17581 tttaactaac acattggaaa tggcagcaga taatcgtcct ttagctggtt ctttcattat
    17641 tttttttgta atagctaatg cagcttcgct atcaatggct tctaatgctt ctaaacttgc
    17701 accagcaata ctgtaagatg aatcatatac acttttttca aaaatgcttc tgtatttttt
    17761 attttctaat tttgctagaa agtcaattgc actggatctt actttactat tcttatcttt
    17821 tattgccatt tcctctattc tttgtataat attttctggt acagttgttt ttgctaatgt
    17881 ttgaatagct tttattctaa atggagcata agtatcattt aatacttctt ctaaaaaaac
    17941 aattgactca gcatttttaa tttgcttact tgcaaaatca atagcttctc ttctgtctaa
    18001 atatttacca gcatttttat attgataaat atattcttta agtgttttat tatctttttt
    18061 attacataac aatattttat ctccatcaac attaataaga tctggtttgc ttgataccga
    18121 aaagctaacg ctatcttctc tactattaag caaaaactcg tacctgtttt tttctttacc
    18181 taaataaaca tctatgctaa atggtaaggt aaaaatttta tctccactct gtgtttgttt
    18241 aatatataca gtagcttttt tattatcctc attataagca taagaaatat ctaattgtgg
    18301 gtgtccactt ccgtaaaacc actgattcca gtaccaattc aaatctttac ctgttacttc
    18361 ttcaaaagct aatcttaact tatcggcatt accagtgcca aatttattat tggttaaata
    18421 tttatttaaa gatttaaaaa aagcatcatc accaacaata tttctaagca tgtgtaaaat
    18481 tctgccccct ttattatagc ttactgcatc aaacatatct tctttatctt tataaaaata
    18541 ccttaccaag tccttcttag agctgccact cattaaatat ccctgcatgt cattataatt
    18601 ctcttcatcg cctgcatctt ttccatattt atattctgcc cataaagttt gactatagtt
    18661 tgcaaaactt tcatttaccg ttaaattact ccaactttca gctgtaacat aatcgccaaa
    18721 ccaatgatga aaaagttcat gagcaatggt tccttcccaa tgattttcat caacaagttg
    18781 ccttgcatct tgctgtgcag actcttggtg tagtgtagct gtggtatttt ccatagcacc
    18841 acttacataa tctctaccaa caatttgtgc atacttattc caagggtatt ctacacccaa
    18901 tattttcgaa aaatattgca tcatttctgg tgtgttgcca aatatttttt tggctacttt
    18961 ttcatattcg tgttctacat aataactcaa ttctttgccc ttataattat cttttactac
    19021 tgcataatct ccaataccca taaaaaataa atagggtgca tgtggtttat ccattttcca
    19081 ataatctgtt cttgtgccgt ccgtattttt aacttggcta actaatttac cattacttaa
    19141 tgttacatat ttatttggga caatcatgta aatctcttga gtagtttttt ggtttggcct
    19201 atcaattgta ggcacccaaa cagatgtagc ttctgtttca ccttgtgtcc atacctgtgt
    19261 gggtttattc ttctctttac cctctgggtt tataaaatat aatcctttag catcagttat
    19321 agcagaactg ccttccactt tcagttcatt gggttttgca gtatattcaa tataaacagt
    19381 atatttttct gttcttttat aggttttatc taaatttatt ttcaatacca ttccatcata
    19441 attatatttc aacttttcac ttttgccatt tttaataatg tctaatttat gtatttccat
    19501 tccttttgca tctaatgcta aagaatcggt agcataaaaa tgaggctgaa gtgttatcca
    19561 agccttgcca agtaagtagc ttttactgta atcaaatttg gcaaccaact tggtatgcac
    19621 taagttgtta attttttctg gaaatgctct gtactgtact ttccagctat catctttggt
    19681 tttttggctt ccagagtgtt gtgccaataa aacagtgtaa caaaataagc taaagaatag
    19741 aaaaattttt ttcatgaatt gtgttttgtg tataaagata aaattgttgc aaagtaacgg
    19801 attggttatt ttacgaaagg ttaaatatta atcatttaat tatcaaatta tatgtttatc
    19861 ggtatatcca tatttttaca taaattcgtc tatcgtttac caatttcaat gaaatacttt
    19921 aaacttaaaa ctttaatttg tttagttatt gttctattag cgttttcgga tgctaatgca
    19981 caaaaaaaac attttatttt tgtacagtca gagaataatg agccttttta tattatgata
    20041 aataataaaa attatagctc tactttatct ggttacctta taattccaag gctaaaaaat
    20101 ggtaaatatt tttttgttac aggcttccct aaagagaaat atcccgagca aaaattctcc
    20161 tatataatca atgataaaga tgtaggcttt gttttaaagc aaaatggcaa aagtggctgg
    20221 agccttttta atgtagtaga ttttacaaat attatggcaa atgccccaga ttgggaaact
    20281 gataaaatta aaaatgatac aatacaaata gaggatatat atgctataaa tacttcttca
    20341 aaaccttcca cccacagttc atcagaagtc ataataaatt caactacacc aacaacagaa
    20401 aatatttctc ctaaaccttt agcccatgta aataaggata ctataaaatt tcaaacaaat
    20461 accgtattaa gtacaaataa tagcaccaat catacagcta aagaagttga aagtaccaat
    20521 acaacaaata ctcaaacaag tacttcaaaa aataatatct cagtaaattc tgaaaagaaa
    20581 ataattaaaa cctaccaaaa gaacgacatc aacggaatag atgaaatgta tattgattat
    20641 acaactaacc cttcagatac aattattatt tttattccta taaacgcaca agaggaaatt
    20701 caagatactc aagaaaaaca caatacagat aaaataaatt attccaattc accaaaaagc
    20761 aaaaatagtt caaatacaat ttctgcttcc aattcaactc aatataatac ctcatgcgta
    20821 tatttagcta cagaggcgga ttatttgaaa actcgtaaaa taatgtccta cgaaacatct
    20881 gatgataaaa tgataaaagc tgcaaaaaaa tcttttacta ataaatgtta ttatgtagaa
    20941 caaatacaaa aactcggact attatttata tcagaacaaa gcaggttaaa gttcttcaaa
    21001 gaagcctatc caaatgtgta cgataaatct aattttgcat ctttagaaat acaatttaca
    21061 atttcaagcg taattaatca atttagacaa gttaaataaa aatgcctcgt tattttatag
    21121 agttagccta ttgtggtaca aactatagcg gatttcaaat tcaaaaaaac gcaagcacaa
    21181 ttcaagaaaa aatcgaacaa gctcttaaaa ccataatcaa acaagaaata aatcttactg
    21241 gatcatcacg tacagatgca ggtgtacatg cacaccaaaa tttctttcat ttcgatattg
    21301 aacaaaaagt taaccaacaa attatttata acctcaatgc cattctccct aatgatatag
    21361 ccataaaaaa aatacattta gtaactccca atgcacattg taggtttcat gcaacatcac
    21421 gagagtatca gtaccatatt tatcagcaaa aaaatccatt ccttcaaaac caagcttggt
    21481 tttaccccta tccactaaat attaatatac tcaatcaagc cgcaactata atagcgtccc
    21541 aaaataactt ttcaactttt tcaaaaaagc atacacaagt aaacaacttt aattgtacaa
    21601 tttttaaatc tcaatgggca caaaacaaca atataattac atatacagta gaggcaaaca
    21661 gattcttaag aggtatggtt agaggcttgg taagcacaat gcttttggtt ggtataaata
    21721 aaataacact acaacaattt caaaatatta ttaattcaca taattgctcc aatgcaaatt
    21781 tttcagcacc agcacaaggt ctattcctca taaatgtaaa atacccttca gaccttttct
    21841 tataaaatat ttatgaagca agctaaatca actctaagca tcaatagttg cgtcgcaccc
    21901 ttgtactttt agtacactta gcagcaaatg tttttaaatt aaattggcat atttcaattc
    21961 aaagtttttt ataaaaaaat ttctaacgta ttttaattaa ccaacaaaca aattaaaatc
    22021 tattttcttt tttaaacctt attaaaaaac aaccagaaaa ataaattgaa ataaaaaaac
    22081 gcttaaaaca cctacaaaca ttgggcttta attaaaacta aaaattattt caataaaaat
    22141 atttggaagt aattaattca cgcttatctt tgcaccccac ttttgaaaaa gtaagtatta
    22201 caaaagcaaa agttctttaa aacaaaaaac attagaatat aattttacta tatttttaaa
    22261 attaaatttg gtagtaaaaa aaatacttct atctttgccg cccgttcaac aaaaacgggt
    22321 agttctaaaa aataaattat ttaaattttt tttaaaaaat cttaagaaaa atttggaaaa
    22381 taaaaaaagg ctcttacctt tgcaacccca aacaaaaggg gtggtaaaaa agccacaaag
    22441 ttctttgaaa gtttgaaagt aacagcactc tgaattttaa taattcaggg taaggtttta
    22501 caagcataaa tttaattata tccgacgtta attttaattt gaagttaata gtcagatcaa
    22561 ctcatttaca atggagagtt tgatcctggc tcaggatgaa cgctagcggc aggcttaata
    22621 catgcaagtc gtggggcagc atgagtagca atacttgatg gcgaccggca aacgggtgcg
    22681 gaacacgtac acaaccttcc ttcaagcggg gaatagccca gagaaatttg gattaatacc
    22741 ccataatata tataagtggc atcatttgta tattaaagct tcggcacttg ttgatgggtg
    22801 tgcggctgat taggtagttg gcggagtaat agcccaccaa gcctacgatc agtagctgat
    22861 gtgagagcat gatcagccac acgggcactg agacacgggc ccgactccta cgggaggcag
    22921 cagtaaggaa ta
//
LOCUS       DAOQPK010000008        22293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_1115_length_22293_cov_5.296115, whole genome shotgun sequence.
ACCESSION   DAOQPK010000008 DAOQPK010000000
VERSION     DAOQPK010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 22293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22293
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1115_length_22293_cov_5.296115"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1493
                     /locus_tag="PLU36_00825"
     CDS             <1..1493
                     /locus_tag="PLU36_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45322.1"
                     /translation="NKTEEAEKERKNALAQKHLAVENANEAEKQKQKAEENATEAINQ
                     KTEAEKQKQKAEENAIEANKQKKNAEEKTIEAEKQKQKAEENATEANKQKKKAEEKTI
                     EAEKQRKAAEEQRKISTKLKDLAEAKNLANLSLQKFREGNKTESKKLAINAFLLNKEN
                     DGPKQNAEIYTALNTNWADAIKNKNQATYHKYAVKNIIGKSNNNIVFTADEAGFIYTA
                     KEENGILQTIGKPILLKEDIRALAVTKDNTKLFVATANGNCFIFNCSSNAQTTLTNNF
                     KINGIAKSIAVINNQTFIIITDKEILKVTTDKASIMQASLQRSGLTSLVVGDKLNKIF
                     VAENNKLFIYNTYNDLVNNQISNTYTLPSKITSLAINENENFIAAGTYDGTLWLKETN
                     SHNNPTTATIHKSSINCIQFKKLKNGVWQIATGSADHTIKLMDVQQLVQHKQTEDIIT
                     LSDGGHTKWVYGIWYSNNGEHLYSCSEDKKIIGWKTTMDGLYKALQ"
     gene            1813..3648
                     /locus_tag="PLU36_00830"
     CDS             1813..3648
                     /locus_tag="PLU36_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45323.1"
                     /translation="MLVVLLISVSTQAQSTSYNSNTTPINGANCTAFGFGALTANTTG
                     FNNVANGSYSLFSNTIGNYNTANGTSALYSNTTGWYNTANGNQSLFNNTFGESNTANG
                     YLSLFNNTTGSANVANGSFALYSNTTGWYNTANGVGSLYSNTTGSANVANGYKSLYSN
                     STGSANVANGNYSLYSNTNGERNVANGDSSLYYNTTGYYNVANGYQSLFNNTFGFYNT
                     ANGYQSLLNNTFGNYNTANGGGSLYSNTTGIGNVANGYQSLYSNTAGHLNVANGSSSL
                     FSNTTGYDNVANGSGSLFSNTTGYNNVANGSGSLFSNTTGYDNVANGRNSLFSNTTGY
                     NNVANGIVSLSSNTTGNYNTANGSYSLAANTTGIDNVANGSYSLFFNTTSSGNVANGY
                     KSLYSNTTGSTNTANGFQALYYNSTGVNNVAIGGMAGFNSLGSNNVYIGSQAGQTETG
                     SDKLYISNGSTNAIIYGDFATHQLLLGVPNATGYVFKGNRTLNVMGGILTDSIRVALS
                     SEWADYVFDDNYNLKPLPELEQFITTYKHLPNIPTTQEVKEKGVNLAEINVKLLEKTE
                     ELTLYIIQQNKRLNEQQKHINGQQEQINNQQKQIEELKNLILNKK"
     gene            3690..5549
                     /locus_tag="PLU36_00835"
     CDS             3690..5549
                     /locus_tag="PLU36_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ45324.1"
                     /translation="MKSIKTYLVLIVIHLAIKQAHSQLYVSSGANITIQDGAKITLQS
                     TNLINNSNITGAGKLILSGNSPAQISGIGNIDNLAIDNTAGISIASIVDTVRIIQSLT
                     PMSGTFTVNGNLVLVSNAVGTARIAAGTGSYINGNITVERYIPAKATRKWSFISSPVT
                     GTINNTWQQLIHITGSGTGGTVCPFLTAHSNGFDATVSNAANIFTYDASKTINTRWEV
                     PSGTLLNTVGMGKGFRVNVRGYRSIGCNLINGNPAYLSPTSVTLRATGAISNTDKNLG
                     SFSIVYPNNGINNYVFIGNPYPSPISFNAFKTTNSSSITNTYAIYIPASPSGVYSYWD
                     GVSFTGGTGYNNATGNIIASGQAFFVQSSVAGNLNLTFDEAHKTDGSDVGYFRTRNFN
                     DKIRVHYLMQDNTQLDEIIVRYAMDAAINNTDENEWDIPSINSGSHYLTSFKGYKGMV
                     VQTRNSNTLASDTVRLNVVCNQSGIYKLGFTEFEHFIGTDIFLIDHLQQSIHNVKQLP
                     VYEFTVDKDKPETKGAGRFSVVFNKLLQNNIVNYSIKMYPNPTSKMVTIELPDNSNYV
                     INIKDITGKIVLQKNTTGGANQFNISKLTSGTYLVEVTDRKRNRATEKLLVEH"
     gene            5822..7297
                     /locus_tag="PLU36_00840"
     CDS             5822..7297
                     /locus_tag="PLU36_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45325.1"
                     /translation="MKTINLLKGITSMLVTLLISISTYAQNTSYSSNTTPINGTNCTA
                     FGVGALTSNTSGNYNVANGGNSLFSNTTGSSNVANGYQSLYSNTTGYANSANGYQSLY
                     SNTTGSYNIANGYQSLYFNTTGGNNVANGYQSLFSNTTGYDNTANGSYSLYANTSGNN
                     NTASGRFSLGSNTTGYYNVANGNNSLFNNTTGHDNTANGFYSLYANTTGINNTANGSF
                     SLLANTTGGNNTANGCQSLYSNTTGYKNTANGYQSLYSNTTGYQNTANGSNSLHSNTT
                     GGNNTANGFQSLYSNTTGRNNVANGANTLRNNTTGLKNVAIGDSTGYNNSTGNYNVYI
                     GSSAGFSETGSNKLYISNGRTNALIYGDFATHQLLLGVPNATGYVFKGNRTLNVMGGI
                     LTDSIRVALSSEWADYVFDDNYYLKPLHELEQFITTYKHLPNIPNAQEVKDKGINLAE
                     INVKLLEKTEELTLYIIQQNKRLNEQQKQIEELKNLILNKK"
     gene            7508..10093
                     /locus_tag="PLU36_00845"
     CDS             7508..10093
                     /locus_tag="PLU36_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ45326.1"
                     /translation="MTKQFFLTLVLFCFFAPSNIYAQEACNANTISEAESYYNIGRFT
                     ECILKLKQCISQSNSFTYDDKLQAYRLIAMSYLAIDSTDAADESIVQLLLLKDDFTAD
                     SRDPERFRYELIKVRANQRINIVSSVSKKAEDIRRAPATISVISKEDIIKRGYSDLIE
                     VLADLPGFDISMLYGVNYANAYQRGLRTTTMEKTLLLIDGVEENDLWTNTADISRQYP
                     ITNIKRIEVIYGPASTMYGPNAFVGVINVITKYPEDIIKQGNQFGLHANVGYGSYNSK
                     YADITLAYKKNLFSAIVTAKHYVSNRHNMSSQSYFDYNSDVYNSVNYKSIMDIKTNAQ
                     AYINTNNLPANHPYYTIYSTAGTADSIVLTQAGINAARSLDKSAYTASINGKQVGFAN
                     PTTLTLLNGKINVGDFSISMQHLVKNEAAGTLYTDLAYAINNVFWIPKRTSIAVSYER
                     KLSNKLSFSSLSSYRINGIDNNTRVSSLYNYARKNLGIKDLIKDSLPGYNTTYFFQNN
                     KQFRSEIKLLYSPTQNLYIIGGVEYRNSQLQGNYLNTTNKLFPEENGTANTGTVLGGN
                     QYNINDIGIYTQANYKFAKYFGITAGARVDNNTIRTHGGFGTEISPRLVVDFAKNNWI
                     FKTIYSRGIMNVSNFTKFGVASGRIPNPNLGTESIKNFEFFISKKINQRLTADIVFYQ
                     SIIKDVVGVKILTPTSSQNQNLGEFKIFGIQSNLYYKIKNLQFVLNYTYTNPKQTIDD
                     AGNKANLTVADIAKHHINAIVNYTFLDNLNINFRLNYISNKYAGVGTTVATNPQNVFP
                     GYTIANSTIGYTIIKGTTVQLVCNNIFNKYYFNPAGRAADGINTPSSLLQPGRNFFIK
                     LNYEF"
     gene            10112..>10973
                     /locus_tag="PLU36_00850"
     CDS             10112..>10973
                     /locus_tag="PLU36_00850"
                     /inference="COORDINATES: protein motif:HMM:NF014609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HMZ45327.1"
                     /translation="MKNIKSEKAVYKIVKAYAFTAIDKIENKKVIGNPASIVICNKRF
                     PPQTQMQQMAVKEKQPMTAFVLHEKENNFSIKYFTPLGIEFGLCVHATLIASSVVQQE
                     FGYTNIHFNAHKISLSLTLSNSFLSTNHTVAQIKLPTYMPVLVNHKLPLHYLKIIGVE
                     QQDIKYVYKCDELKDEIIVLNNAEILRSVKPNFLELSKVLTTSNTRGLFLTSLSTIKN
                     IDYEVRIFAPHFGIDEDISCGSANCSLIPLWHKITNEVGNKEYKIICPYNGKDNVIGG
                     VELGVFDVENN"
     assembly_gap    10974..11073
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11173..11727
                     /locus_tag="PLU36_00855"
     CDS             11173..11727
                     /locus_tag="PLU36_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45328.1"
                     /translation="MKICFQINNIIAEVEIKQPLISDIPYLQNLNSKWQFNALNGNTE
                     KGFLSGLFDEAFFRTLIKEEAIIAAYSNNTLIAYMLTANNANLNILKTHKEEVEKLKN
                     NLVINKNAKVAIGIQTAVEEDYHGSGLIVAIRNEFRKMLCNKFQYLFTTISKNNQRSF
                     ASATKYGWKKVGEDEHHYFLLLTV"
     gene            11742..14294
                     /locus_tag="PLU36_00860"
     CDS             11742..14294
                     /locus_tag="PLU36_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45329.1"
                     /translation="MIWNLIIKRYTDIRSDERKPVSILFLHSLLLGFSTSLFFVAANS
                     YFIKNVKINNIPYAYIAAGILGILLVFLFKYLIKVIGSIFTFFTVLVLFAITCFALYI
                     GHLYLDSTSLLSLVLAFLGFIFIFPFSGLFVLGFTGICLQLFNLHQNKRLLALIGVGE
                     VVASIIGYLMVPIITKFTGNTVHLFLFSGIFILASTRPIWHIYKINIKTFSVTPGSKT
                     IKRNYVGIFKKDRFYRSLAIVTFFSVLAVYFSDYAYLISVRKLAQLTSMDVANIVAVL
                     FCIIKIGELLFSFFSAKIISTIGMKFSLLLLPFMLILFSTLSILAFSMFKEVLIFVIL
                     FFLMSKWCDRVLRKGVYAPSSKVMYQVVEPVERMQLQTTIEGTISQFSIVLSGLILLL
                     VSKLKLTTNNDVLFYSLTAISALFSICWFVMAWLLFNNYKTKIHDYLHNINGIKNTVQ
                     KQNTSILINQWVNNTEFGLSIEDKNSILQVYDNVQMDTVNMQKIMEHIAFYNPSLQSF
                     LANNDSTLLHKKIVRLYFTNDHFFSRVLIIKYLQQNNQLSDFNIFYELYEAADFEIKK
                     IIISSLNQLNYTTQNNQYYFYDLCKIFVQEIVWAELSIYDTINVPNHHLHTLLHEYAT
                     ECKNHLLNILKVLYGNNVITIVQQVLNENDKAAEGSIFAVELLDNILQQPLKEKIIPV
                     FEPIPQQMRWAKLGTFCNIYHLSEEQRLKEILIKNFNLINIKLKQTALESYYNLTQNK
                     EVLKAFSTSNLEEMKATAEILNNKENPNPYFSKEEFLNKLQLNSIVNQYQLQYLCRFA
                     IGLENENKLNNNFIAVNKTTALEYILKVKANNNQKILLDIIGLSVLFRLQQE"
     gene            14400..15230
                     /locus_tag="PLU36_00865"
     CDS             14400..15230
                     /locus_tag="PLU36_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HMZ45330.1"
                     /translation="MIETILLPSDFSATAVNAGIYAIELAQQVGAKKIVVYHTYDAAS
                     VSDPMVSYTQKIVTEPFRQKSAAQLNEFIDKLNAKNKSSIVIEAFHSYADLKDALNEL
                     IASTGAQLIVMGITGGGVFKETFIGSNSVNVAKSTNIPVIIVPTETSFSPISNILLLS
                     DLNNVEDTTPTEAIKNILDASKAKLHVLNVTDNTEKAEASSDKQQLDKLFEAYNPNFS
                     FMSNPDFVGAVDWFVKEKGIDLVIVIPKKHGLFETLFKFNHTKTLAFHGKVPLMAVKK
                     "
     gene            complement(15283..15843)
                     /locus_tag="PLU36_00870"
     CDS             complement(15283..15843)
                     /locus_tag="PLU36_00870"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HMZ45331.1"
                     /translation="MKKTFIVLTTMAMYLLTACSGEQTTTGSESTTQTTDAVGQSGVV
                     DDQSAKNIVQTAAGSKDHTTLVAAVKAAGLLDALSNAGPFTVFAPTNAAFDKLPAGTV
                     EDLLKPENKGKLENALGYHTYVGSIKAEMMQDGQELNMVFEGNVTITKKDGKTFVNGS
                     EIVASIPTSNGMIHVINDVLMPPAKK"
     gene            complement(15966..16673)
                     /locus_tag="PLU36_00875"
     CDS             complement(15966..16673)
                     /locus_tag="PLU36_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ45332.1"
                     /translation="MIENKACILLVEDEEGLNETLKLNLEMEGYDVTSAYAGDEALTE
                     LSKAHFDLMILDIMLPEIDGITVAQTVRLSNDELPILMLSAKNSGADRVLGLKKGADD
                     YLTKPFNLEELLLRVEKLIQKNKKIYDKESTLGDTYSFGNNLIDFKSLDAITYDGKKI
                     KLSKKETNLLKLLIENKNEVVPREKILLVVWGYNVFPTTRTVDNFILMFRKHFEENSK
                     QSKHFHSVRGIGYKFTD"
     gene            complement(16677..17651)
                     /locus_tag="PLU36_00880"
     CDS             complement(16677..17651)
                     /locus_tag="PLU36_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ45333.1"
                     /translation="MKSFFHKKLALITTVYWFLLLYMIAALLFWFIKLNHHASDTAEM
                     QIALLQTSDSLYKEKISTIEATKKRKIAQYLGEGTTFLAVILIGAVFVYRATRKQIRL
                     SVQQQNFMMAVTHELKTPIAITHLNLETLQKRKLDEEKQQKLISNTIQEANRLNSLCN
                     NILVAAQLDGGAYYSNTYELNLSDLTETCIEDFAIRYPQRKLEADIQQQLFINGEALL
                     LQMLVNNLIENALKYSPKEMPVTIRLYTSNKQTFLDVLDKGCGVKPQDRKKIFDKFYR
                     SGSESTRTTKGTGLGLYLCKKIMKSHNGNITMTNNHPQGSIFTAVFNM"
     gene            complement(17651..18106)
                     /locus_tag="PLU36_00885"
     CDS             complement(17651..18106)
                     /locus_tag="PLU36_00885"
                     /inference="COORDINATES: protein motif:HMM:NF025474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HMZ45334.1"
                     /translation="MKFTAYLFFVMAFFSSCTTLDVYERTTFFNNHQWKSQQPVQAVF
                     EISDTTTLYNLYFVLRHEDAYKYNNIWVDISMQSPDTTISVKRNFVLGDNEKWLGSSM
                     SDIIEHRIKFSTAPAKLKKGKYIFTLHQIMREDPLHSILNAGIRVERTK"
     gene            complement(18155..19030)
                     /locus_tag="PLU36_00890"
     CDS             complement(18155..19030)
                     /locus_tag="PLU36_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HMZ45335.1"
                     /translation="MLYSMTGYGRGEKIIGDKTFLIEIKSLNGKQFDLRLFLPNLLKP
                     FEFEIRSKIAEDLERGSVECVITIKQNGINKPVSINTELLKAYYKSISEIAEELNADK
                     TQLMAAILRLPEVVSNSTEILSDTEWKEVEALLKNVLSDINEHRKEEGKSSECDLLQR
                     LVSIEKLQDEVKTLEPLRREKIKEGLQKLLIEYVGVNNYDTNRLEQELIYYIEKIDIS
                     EEQVRLINHCNYFRNILEEPTKAKGKKLSFILQEFGREINTTGSKANNADIQKKVVLM
                     KDELEKMKEQVLNVL"
     gene            19220..21298
                     /gene="ligA"
                     /locus_tag="PLU36_00895"
     CDS             19220..21298
                     /gene="ligA"
                     /locus_tag="PLU36_00895"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HMZ45336.1"
                     /translation="MYSNKEQWLQQSKLFLSNSLNSQFVLSNINTLRAVLQFHEYNYY
                     VLNNALISDNEYDFLFNLLTKAEKENPEIITKDSPTQRVGSSLNNGFETVQHLVPMLS
                     LENSYNETDLLDFDRKAKEASGLQEIEYCVEPKLDGASISLIYENDLLTRATTRGNGV
                     EGDDITTNIKQIRSIPLSAPFSKYGIRQIEIRGEVMMTKEAFQKYNEKLTQQGLATVA
                     NPRNTAAGSLRIKDPKEVAQRNLDAFLYHISYITQNNTHKVNTHSQQLNLLWNCGFRS
                     TENEKFVAKNINEVIEYVTSFEQKRDDLPYEIDGMVIKVNSLLLQEQLGMTSHHPRWA
                     IAYKFKARQATTVLNAVEFQVGRTGAITPVAKLEPVFIGGVTVSSISVHNVEYIKEKD
                     LKIGDTVLIERAGDVIPQIVKSITTARKGNEHEIVFPENCPVCKNQLFKEEGEAVWRC
                     INIECEAQVVEKIIHFASKDAMDIKGLGDAIVRKFYEKNLLKDIPTIYKLNLTNIGEI
                     EGFGKKSIDNLQAAIASSKQQPLNRLIYGLGIRFVGETTAKTLANSVEHLLEFKNKTT
                     DDLQTLEDVGTKVAKSIHHFFSNESNINMILELEQLGLQLKNTKKQTINIGELAGQTF
                     LFTGTLSKLKRNQAEALAEQHGGIIVSGVSAKLNYLIVGDDAGSKLEKAKKINTIKIL
                     NEEEFLQMIG"
     gene            complement(21310..21738)
                     /locus_tag="PLU36_00900"
     CDS             complement(21310..21738)
                     /locus_tag="PLU36_00900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HMZ45337.1"
                     /translation="MKNLNLVIDTNIFLVSIAKNFRYRWIYDALLNGKFNLLISTEIL
                     LEYEELFQIKYGVKYSSDKFVEFLLNKNVLLISPSFRWQTIYPDLDDNKFVDCAIAGN
                     ADFIISNDADFKILKTIDFPKVNVLTLDEFETSFKEYFIS"
     gene            complement(21725..21952)
                     /locus_tag="PLU36_00905"
     CDS             complement(21725..21952)
                     /locus_tag="PLU36_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45338.1"
                     /translation="MNTPNVQIPPSTKFSNVQLELLNLFAHNVSDEELIELKDVLGKR
                     FLSKLSNDVNKAIEEKNITNNDLEKWLNEKS"
     gene            22112..>22293
                     /locus_tag="PLU36_00910"
     CDS             22112..>22293
                     /locus_tag="PLU36_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45339.1"
                     /translation="MERKIKIFKTFEEQEAYTLQQQGKTTPLERFRNLFYMQQLSKKF
                     NSVKNSTRKITVHHGY"
BASE COUNT         8019 a         3433 c         3390 g         7351 t
ORIGIN      
        1 taaataaaac agaggaagca gagaaagaaa gaaaaaatgc acttgctcaa aaacatttag
       61 ctgttgaaaa tgcaaacgaa gccgaaaaac aaaaacaaaa agccgaagaa aatgcaacgg
      121 aagcaataaa ccaaaaaaca gaagccgaaa aacaaaaaca aaaagccgaa gaaaatgcaa
      181 tagaagctaa taagcaaaaa aagaatgcag aagaaaaaac catagaagcc gaaaaacaaa
      241 aacaaaaagc cgaagaaaat gcaacagagg ctaataagca aaaaaagaaa gcagaagaaa
      301 aaaccataga agccgaaaag caaagaaaag cagcagaaga acaaagaaaa atttcaacta
      361 aattaaaaga tttagccgaa gccaaaaatc ttgccaacct ttctttacaa aaatttagag
      421 aaggtaataa aacagagagt aaaaagcttg ctatcaacgc ctttttgctt aataaagaaa
      481 atgatgggcc taaacaaaat gcagaaattt ataccgcact aaacaccaat tgggccgatg
      541 caataaaaaa taaaaaccaa gcaacttacc ataaatatgc agtaaaaaat ataataggca
      601 aatcaaataa taatattgtt tttactgcag atgaagcagg ctttatatat acagcaaaag
      661 aagaaaacgg aattttacaa accattggta aaccaatttt acttaaagaa gatattaggg
      721 ctttggcagt aactaaagat aatacaaaac tttttgtagc cactgctaat ggcaattgct
      781 ttatttttaa ttgtagtagt aacgcacaaa ctacactcac caataacttt aaaataaatg
      841 gtattgctaa aagtatagca gtaatcaata atcaaacatt tattattatt accgataaag
      901 aaattttaaa agttactacc gacaaagcct ctattatgca agcaagttta caacgcagtg
      961 gattaacctc attagttgta ggcgataaat tgaacaagat ttttgtggca gaaaataata
     1021 aactttttat ttataacaca tacaacgatc ttgtaaataa ccaaataagt aacacttata
     1081 ccttaccatc taaaattaca agtttggcta taaacgaaaa cgaaaatttt atagctgctg
     1141 gcacttacga cggaacttta tggttaaaag aaaccaactc acataataac ccaacaactg
     1201 ccaccataca caaatcaagc attaactgta ttcagtttaa aaaattaaag aacggagttt
     1261 ggcaaattgc tacaggtagt gcagaccata ctataaaact tatggatgta caacagttgg
     1321 tacaacacaa acaaaccgaa gatattatta ccttaagcga tggtggccat acaaaatggg
     1381 tttatggtat atggtatagc aacaatggag aacatcttta ctcttgcagc gaagataaaa
     1441 aaattattgg ctggaaaaca actatggatg ggctttacaa agctttacaa taattattaa
     1501 actattagca aaaacacttt taaaacgttt taaagtttta acgaagtaat tatttaaaac
     1561 attactactg ttttgctttt ttactacaaa caatatacat acgcaataag cctgtaatgc
     1621 caatactatt acagaggcag aaaattatta ttatagttta gttataaggg tttttaggca
     1681 tatttccaaa aaatgcctgt cacatgatat ttattatttt aaaaataatg actactatac
     1741 tttagtataa taattcttaa ccaaaaaatt acttttatga aaacaataaa tttattatag
     1801 ggtattacct ctatgttggt ggtattactt attagtgtaa gcacacaagc acaaagtaca
     1861 tcttacaata gcaacacaac cccaattaat ggagcaaatt gtactgcatt tggttttggg
     1921 gctttaactg ccaacacaac cggatttaat aatgttgcta atggtagcta ctctctattt
     1981 tccaatacaa ttggaaatta caatactgct aatggtacaa gtgctcttta ttctaataca
     2041 actgggtggt acaatacagc taatggtaat caatctctat ttaacaatac attcggagaa
     2101 tccaacactg caaatggtta cctatctcta tttaacaata caaccggtag tgccaatgtt
     2161 gctaatggta gttttgccct ttattccaat acaactgggt ggtacaatac agctaatggt
     2221 gtaggctctc tatattccaa tacaaccggt agtgccaatg ttgcaaatgg ttacaaatct
     2281 ctatactcca attcaaccgg tagtgccaat gttgctaatg gtaattactc tctatattcc
     2341 aatacaaacg gagaacgcaa tgttgcaaac ggtgattcct ctctatatta caatacaacc
     2401 ggatattaca atgttgcaaa tggttaccaa tctctattta acaatacatt cggattttac
     2461 aacaccgcaa atggttacca atctctattg aacaatacat tcggaaatta caacactgca
     2521 aatggcggcg gctctctata ttccaacaca accggaatag gcaatgttgc taatggttac
     2581 caatctctat attccaatac tgctgggcat ttaaatgttg ctaatggtag tagctctctt
     2641 ttttccaata caacgggata tgataatgtt gcaaatggtt caggctctct tttttccaat
     2701 acaacgggat ataacaatgt tgcaaatggt tcaggctctc ttttttccaa tacaacggga
     2761 tatgataatg ttgctaacgg acgcaattct cttttttcca atacaacggg atataacaat
     2821 gttgctaatg gcatcgtctc tctatcttcc aatacaacgg gaaattacaa caccgcaaat
     2881 ggtagctact ctctagctgc caatacaacc ggaatagaca atgttgctaa tggtagttac
     2941 tctctatttt tcaatactac tagttcagga aatgttgcta atggttacaa atcactttat
     3001 tccaatacaa caggctctac caacacagca aacggttttc aggctcttta ctataattct
     3061 actggggtta ataatgttgc tattggtggt atggcaggct ttaacagcct tggcagcaac
     3121 aatgtgtata ttggtagcca ggcaggacaa acagaaacag gcagcgataa attgtacatt
     3181 agtaatggta gtaccaatgc cattatttat ggcgattttg ctacgcacca gcttttattg
     3241 ggtgtgccaa atgctacagg gtatgttttt aaaggtaaca gaactttaaa tgttatgggt
     3301 ggtattctta cagatagtat tagagtggca cttagcagcg aatgggcaga ttatgttttt
     3361 gatgataact ataatttaaa acccctaccc gaattagaac aatttattac cacatataaa
     3421 catttaccca atatacctac tacacaggaa gtaaaagaga agggtgttaa ccttgctgaa
     3481 ataaatgtaa aacttttaga aaaaacagaa gaacttactt tatacattat tcaacaaaac
     3541 aaaagattaa acgaacaaca gaaacatatt aatggccaac aggaacaaat taataaccaa
     3601 cagaaacaaa tagaggaact aaaaaactta atattaaata aaaaataaat tgttactttt
     3661 atataattta aaatttaata aataaaccaa tgaaaagtat aaaaacatat cttgttttaa
     3721 tagtaattca tttggctatt aaacaagctc acagccaatt gtatgttagt agtggagcaa
     3781 atattaccat acaagatggt gcaaaaataa ctcttcaaag tacaaacctt attaacaata
     3841 gtaatataac tggtgcagga aaattaattc tttctggcaa tagccctgca caaataagtg
     3901 gcattggcaa tatagataat ttggcaatag ataatactgc aggtattagt atagccagta
     3961 ttgtagatac tgtaagaatt atacagtctt taacgcccat gtcgggtact tttactgtta
     4021 atggcaattt agtattagta agcaatgctg ttggaacagc cagaatagca gcaggtacag
     4081 gtagttatat taacggtaat attactgttg aacgttatat accagctaag gcaactcgta
     4141 aatggagttt tattagcagc cctgttactg gtacaataaa taatacatgg caacaactaa
     4201 tacatattac aggttcgggt acaggtggta ctgtttgtcc ttttttaact gcacatagca
     4261 atggttttga tgcaacggta agcaatgctg caaatatttt tacttatgat gctagtaaaa
     4321 caattaatac ccgttgggag gtaccttctg gtactttatt gaatactgta ggtatgggta
     4381 aaggatttag agtaaatgtt cgtgggtata gatctatagg ttgcaattta ataaatggta
     4441 acccggcata tttaagccct acaagtgtta cacttagggc tactggggct atcagtaata
     4501 cagataaaaa cttaggtagc tttagtattg tttaccctaa caatggtatt aataattatg
     4561 tttttattgg caacccttac cccagcccta ttagttttaa tgcatttaaa actaccaata
     4621 gcagtagtat aactaatact tatgctattt atatacctgc cagccctagt ggggtgtata
     4681 gctattggga tggtgtttct tttacaggag gtacgggtta taataatgct acaggaaata
     4741 ttattgccag tggtcaggca ttttttgtgc agtcttctgt tgcaggtaat ttaaacttaa
     4801 ctttcgacga ggcacataaa acagatgggt ctgatgttgg ttattttcgt accagaaatt
     4861 ttaatgataa aataagagtg cattatttaa tgcaggataa tacacagtta gacgaaataa
     4921 ttgttcgtta tgcaatggat gcagctataa ataatacaga tgaaaacgaa tgggacattc
     4981 cttctattaa cagtggcagc cattatttaa caagctttaa aggatataaa ggcatggtgg
     5041 ttcaaactag aaatagtaat acacttgcta gcgatactgt taggctaaat gttgtttgca
     5101 atcaaagcgg aatttataaa ttagggttta ccgaatttga acattttatt ggtaccgata
     5161 tttttttaat agaccattta caacaatcta ttcacaacgt aaaacagttg cctgtttatg
     5221 agtttactgt agataaagac aaacctgaaa caaagggagc aggcagattt agtgtagttt
     5281 ttaataaact attacaaaat aatattgtaa attattctat taaaatgtat ccaaacccta
     5341 ccagtaaaat ggttacaata gaactgcccg ataactctaa ttacgttatt aatattaagg
     5401 atataaccgg aaaaattgta ctacaaaaaa acaccaccgg tggtgccaat caatttaata
     5461 ttagtaaatt aaccagtggt acttacttgg tagaagttac tgatagaaag agaaatagag
     5521 caacagaaaa gttgttagta gaacattaat tacttatcac cgttttaaaa aattaaaaga
     5581 gaagatgttt aaaaacatct tctctttttt attttcaatt gcattttctt gttgccaata
     5641 ctgcaacaaa ataagtggtt atatacaatc tattttttat aaaaaataaa actttgtata
     5701 aacattggtt attaggcttt aagcatatat cccaaaaaca ctttctctta tatttattat
     5761 tttaataata agaaatacaa tactttagta ccatatttct ttactaaata aaattactat
     5821 tatgaaaaca ataaatttat taaagggtat tacctctatg ttggttacgt tacttattag
     5881 tataagtaca tatgcacaaa atacatctta cagtagcaac acaaccccaa ttaatggaac
     5941 aaattgtact gcatttggtg ttggggcttt aacttccaac acatctggca attacaatgt
     6001 tgccaatgga ggcaactcac tattctccaa tacaacagga tctagcaatg ttgctaatgg
     6061 ttaccaatct ctttattcca atacaaccgg atatgccaat agtgctaatg gttaccaatc
     6121 tctttattct aatacaaccg gaagttacaa tattgctaac ggataccaat ctctatattt
     6181 caatacaacc ggaggtaaca atgttgctaa tggctaccaa tctctatttt ccaatactac
     6241 tgggtatgac aatactgcta atggtagcta ctcactatat gctaatacat ctggtaataa
     6301 caatactgct agtggtagat tctctctagg ctccaataca accggatatt acaatgttgc
     6361 taatggtaac aactctctat ttaataatac aacagggcat gacaatactg ctaatggttt
     6421 ctactcgcta tatgccaata caaccggcat taacaatacc gcaaatggca gcttctctct
     6481 attggccaat acaaccggtg gtaataatac tgctaatggt tgccaatctc tttattccaa
     6541 cacaactgga tataaaaata ctgctaatgg ctaccagtct ctatattcca acacaaccgg
     6601 atatcaaaat actgctaatg gcagcaattc attacattcc aatacaaccg gtggtaacaa
     6661 tactgctaat ggtttccaat ctctttattc taatacaacc ggacgtaaca atgttgcaaa
     6721 tggtgcgaac actctacgta acaatacaac agggttaaag aatgtagcta taggtgattc
     6781 aacaggttat aataacagta ctggtaatta taatgtgtat attggtagca gtgcaggttt
     6841 ttctgaaaca ggcagcaata aattgtatat tagtaatggt agaaccaatg cccttattta
     6901 tggcgatttt gctacgcacc aacttttatt gggtgtacca aatgccacag ggtatgtttt
     6961 taaaggtaac agaactttaa atgttatggg tggtattctt acagatagta ttagagtagc
     7021 acttagcagc gaatgggcag attatgtttt tgatgataac tattatttaa aacccctaca
     7081 cgaattagaa caatttatta ccacatataa acatttaccc aatattccga atgcacagga
     7141 agtaaaagac aagggtatta accttgcaga aataaatgta aaacttttag aaaaaacaga
     7201 agaacttacc ttatacatta ttcaacaaaa caaaaggtta aacgaacaac agaaacaaat
     7261 agaagaacta aaaaatttaa tcttaaataa aaaataagtt tagtatgtat taatgcttta
     7321 aaaaaattaa ctgttacttt tatagaaatt taatttttaa aaataaacca aaagcgaaag
     7381 ggagcacttt tgctcttata gccaagagaa aaaacaattg gctgtaaaac agctatggct
     7441 gggctgtaca aggctttacc ataattatta agcaattggt tcagaaattt ttaacagttt
     7501 tatatctatg acgaagcaat tttttttaac attagtactg ttttgctttt ttgcaccaag
     7561 taatatatat gcacaagagg cttgtaatgc caatactatt tctgaagcag aaagctatta
     7621 taatattggc agatttaccg agtgtatttt aaaattaaaa caatgtattt ctcaatcaaa
     7681 tagttttact tacgatgata aactacaagc atacagatta attgcaatgt cttatttagc
     7741 aatagactct acagatgcag cagacgagag tattgtacaa ctacttttat taaaagacga
     7801 ttttactgcc gactccagag acccagaaag atttagatac gaattaatta aggtacgtgc
     7861 caaccagcgt attaatattg tttcctccgt ttctaaaaaa gccgaagata taagaagggc
     7921 tcctgcaacc attagtgtta ttagtaaaga agatattatt aaaagaggtt attccgattt
     7981 aatagaagtg cttgcagatt tgccaggctt tgatatatct atgttatatg gtgtaaatta
     8041 tgccaatgca taccagcgtg gcttaagaac aacaaccatg gaaaaaactt tattattaat
     8101 tgatggggta gaagaaaatg atttatggac caacactgcc gatatttcga gacaatatcc
     8161 tattaccaat attaaaagaa tagaggttat ttatgggcca gcctctacca tgtatgggcc
     8221 aaatgctttt gttggtgtaa ttaatgtaat tacaaaatat cctgaagata ttataaaaca
     8281 aggtaaccag tttgggctac atgccaatgt aggctatggt agttataata gtaaatatgc
     8341 agacattact ttagcctata aaaaaaatct gttctcggca atagtaacag caaaacatta
     8401 tgtttctaat aggcacaaca tgagttcgca atcctacttc gattataatt ctgatgttta
     8461 taattcggta aattataaaa gcattatgga tattaaaacc aatgcccaag cttatataaa
     8521 caccaataac ttaccagcca atcatcctta ttatactatt tactctactg caggtacagc
     8581 agatagcatt gtattaacac aggcaggtat taatgcagca agaagtttag ataaatcagc
     8641 atataccgca agtattaatg gtaaacaagt gggctttgct aaccctacca ccttaacctt
     8701 attaaatgga aaaattaatg tgggagattt ttctataagt atgcaacact tagtaaaaaa
     8761 cgaagctgct ggtactttat atacagattt agcttatgct attaataatg tattttggat
     8821 acctaaaaga acttctattg ctgttagcta tgaaagaaaa ttatctaata aactttcctt
     8881 ttcctctctc tctagctaca gaattaatgg aatagataat aacacaagag tttcttcttt
     8941 atacaattat gccagaaaaa atttgggcat aaaagattta attaaagaca gcttacccgg
     9001 ctataacaca acctactttt ttcaaaacaa caaacaattt agaagcgaaa taaaattact
     9061 ttactctcca acgcaaaact tatacataat tggaggtgta gaatatagaa atagccaact
     9121 acaaggcaat tacttaaata caaccaataa actttttccg gaagaaaatg gtactgctaa
     9181 tacaggtaca gttttgggtg gcaaccaata taatattaac gatattggaa tttatacaca
     9241 agcaaactac aagtttgcaa aatactttgg tattactgca ggagccagag tagataataa
     9301 taccataaga acacatggag gttttggaac agaaattagc ccaaggctag tagtagattt
     9361 tgcaaaaaac aattggattt ttaaaactat ttacagcagg ggtataatga acgtttcaaa
     9421 ttttacaaaa tttggtgtag caagtggcag aattcccaac ccaaatcttg gtacagaaag
     9481 tattaaaaat tttgaatttt tcattagtaa aaaaataaat caacgcctaa ctgccgatat
     9541 tgttttttat caatctatta ttaaagatgt ggtaggcgta aaaatattaa cacctacaag
     9601 ctctcaaaac caaaaccttg gtgagtttaa aatttttggc attcaaagta atttgtatta
     9661 taaaattaaa aacttgcaat ttgttttaaa ctatacttat actaacccca aacaaaccat
     9721 agatgatgca ggcaataaag ccaacctaac cgttgcagat atagccaaac accatattaa
     9781 tgctattgtt aattatactt ttttagataa cctaaatata aacttccgtt taaattatat
     9841 aagtaataaa tatgcaggtg taggcacaac agttgcaacc aaccctcaaa atgtttttcc
     9901 aggctatacc attgctaaca gtactattgg ttacaccata attaaaggca ccacagtaca
     9961 actagtttgt aataatattt ttaataaata ttattttaac ccagcaggca gggctgcaga
    10021 tggtattaac acaccatcta gtttattaca accaggtaga aactttttta ttaagttaaa
    10081 ttatgaattt taatttaaca tgagtttgat tatgaaaaat atcaaatctg aaaaagcagt
    10141 ttacaaaata gttaaagctt acgcatttac tgctatagat aaaatagaaa ataaaaaagt
    10201 aattggcaac ccagcaagta ttgttatttg taataaaagg tttccccctc aaacccaaat
    10261 gcaacaaatg gctgttaaag aaaaacaacc tatgactgca tttgttttac atgaaaaaga
    10321 aaataatttt tctataaaat attttacacc attaggtata gagtttggcc tatgtgtaca
    10381 tgcaacactt attgcatcaa gtgtagtaca acaagagttc ggctatacca atatacattt
    10441 taatgctcat aaaatttctt tgtctcttac attaagcaat agtttcttgt caacaaatca
    10501 tacagttgca caaataaaat tgcctactta catgcctgta ttggtaaacc ataaactacc
    10561 tttacattac ttaaaaatta taggagttga acagcaagat ataaaatatg tgtacaaatg
    10621 cgacgaactg aaagatgaaa taatagtttt aaataatgca gaaatactaa gaagtgttaa
    10681 accaaatttt ttggagttga gtaaagtttt aactacatcc aatacaagag gattgtttct
    10741 tacctcttta tctactataa aaaatataga ttatgaagta agaatttttg cacctcattt
    10801 tggaatagat gaagacatct cttgcggctc tgccaattgt tcattaattc ctttatggca
    10861 taaaataact aatgaagtag gcaataaaga atacaaaata atttgcccat ataatggaaa
    10921 agataatgtt ataggtggag tagaattagg cgtttttgat gtagaaaaca atannnnnnn
    10981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnaaagata atgttatagg tggagtagaa
    11101 ttaggcgttt ttgatgtaga aaacaatagt gcttttatag gaggtattgt tacaagttaa
    11161 ttttatggaa caatgaaaat ttgttttcag ataaataata taatagctga agttgaaatt
    11221 aaacaaccat taatttcaga tataccttat ttgcaaaacc ttaatagcaa atggcagttt
    11281 aatgcgttaa acggaaatac agaaaaagga ttcttgtcgg gtttatttga tgaggctttt
    11341 tttagaacac tcattaaaga ggaagcaatt atagcagcct acagcaacaa cacactaatt
    11401 gcatacatgc ttacagctaa taatgcaaac ttaaacatac taaaaacaca taaagaagaa
    11461 gttgaaaaat taaagaataa cttagtaatt aataaaaatg caaaggttgc aataggtatt
    11521 caaacagcag ttgaagaaga ttatcatggc agtggattaa tagtagctat tagaaatgaa
    11581 tttagaaaaa tgctgtgtaa taagtttcaa taccttttta caaccatttc aaaaaataac
    11641 caacgatcat ttgcctctgc aacaaaatat ggttggaaaa aagtgggcga agacgagcat
    11701 cattattttt tacttttaac cgtttagaat tatattaata aatgatatgg aatttaataa
    11761 taaagcgtta taccgatatt cgttctgatg aacgaaaacc tgtatctata ttatttttac
    11821 actctttact attaggcttc tctacttcct tattttttgt tgctgctaat agttatttta
    11881 ttaagaatgt aaaaattaac aatattccgt atgcatatat tgctgcaggt attttaggta
    11941 ttttacttgt ttttcttttt aaatatttaa ttaaagttat aggaagcatt tttacatttt
    12001 ttactgtgct tgttttattt gccattactt gctttgcttt atacataggg catttgtatt
    12061 tagactctac atctttatta tccctggtgt tagcatttct gggctttatt tttatatttc
    12121 ctttttctgg tctttttgta ttagggttta caggtatttg cttgcagttg tttaatcttc
    12181 atcaaaataa aaggctgctg gctttaattg gtgttggcga agttgtagct tcaataatag
    12241 gatatttaat ggtacccatt attaccaagt ttacaggaaa tactgtgcac ttatttttat
    12301 tttctggaat ttttatttta gcctctaccc gacctatatg gcatatttat aaaattaata
    12361 ttaaaacttt ttcggttaca cctggttcta aaactattaa aagaaattat gtaggcattt
    12421 ttaaaaaaga taggttttat aggtcattag caattgttac tttcttttct gttttagcag
    12481 tttatttttc cgattatgca tacttaattt ctgtacgcaa acttgcacaa ttaacaagta
    12541 tggatgttgc aaatattgtt gctgtacttt tttgtattat aaaaattgga gaattactct
    12601 tttcattttt ttctgcaaaa attattagca caataggcat gaagttttcc ttattgctat
    12661 tgccttttat gttgatttta ttttcaactc tgtctatact tgcttttagt atgtttaaag
    12721 aggtgctgat ttttgtaatc ctcttttttt taatgtcgaa atggtgcgat agggttttaa
    12781 gaaaaggggt gtatgctcct tcttctaaag taatgtatca agtagttgag ccagtagaaa
    12841 gaatgcaact acaaacaact attgaaggta caataagcca attttcaatt gtattaagcg
    12901 gattaatttt attattggtt tcaaagctta aattaactac taataacgat gtattatttt
    12961 attcccttac ggctatttct gctttatttt ctatatgttg gtttgtaatg gcctggttgt
    13021 tattcaataa ttataaaacc aaaattcatg attatttaca taatattaat ggcattaaaa
    13081 atacagttca aaaacaaaat acatctatac taattaacca atgggttaac aatactgagt
    13141 ttggtttaag tatagaagat aaaaatagta ttcttcaagt ttatgataat gtacaaatgg
    13201 atacagtaaa tatgcaaaaa ataatggagc atattgcctt ctacaatcca tccttacaaa
    13261 gctttttagc caataatgat agtacattac ttcacaaaaa aattgtacga ttatatttta
    13321 caaacgatca tttttttagt agggttttaa taattaaata cctgcaacaa aataatcaac
    13381 taagtgattt taatattttt tatgaattgt acgaagctgc agattttgaa ataaaaaaaa
    13441 taatcatatc ctcattaaac caattaaact atacaaccca aaacaaccaa tattattttt
    13501 atgacctatg taaaattttt gttcaagaaa ttgtatgggc agagctttct atttatgata
    13561 ccataaatgt acctaaccat catttacata ccttgttaca tgaatatgca acagaatgta
    13621 aaaaccattt attaaatatt ttaaaagtgt tatatggtaa taatgtaatt actatagtac
    13681 aacaggtttt aaatgaaaat gataaagccg cagaaggaag catttttgct gttgaattat
    13741 tggataatat tttacaacaa cctttaaaag aaaaaattat tcctgttttt gaaccaatcc
    13801 ctcaacaaat gcgttgggca aaattgggta cattttgtaa tatttatcat ttaagtgaag
    13861 agcaaaggct aaaagaaatt ttaataaaaa atttcaacct aattaatata aaactaaaac
    13921 aaacagcctt agaaagctat tacaacttaa cccaaaataa agaagtatta aaggcatttt
    13981 caacaagtaa tttagaagag atgaaagcaa ctgcagaaat acttaacaat aaggaaaacc
    14041 caaatcccta cttttctaaa gaagagtttt taaacaaatt acaattaaac tctattgtta
    14101 atcaatatca attacagtat ttatgccgct ttgcaatagg tttagaaaac gaaaataaat
    14161 taaataataa tttcattgct gtaaataaaa caacagcttt agaatatatt ttaaaagtga
    14221 aagccaataa taatcaaaaa attttattag atataattgg gctaagtgtt ttatttaggt
    14281 tgcaacaaga ataaaaatag actggttgtt tgctataata tatttagcag agaacccata
    14341 taacataaaa aacttttttc ttttcaatca tcgtttatct ttacaccata cattgcttta
    14401 tgatagaaac tattttactt ccttccgatt tttctgcaac agccgttaat gcaggaattt
    14461 acgctattga gttagcccag caagttggag ctaaaaaaat tgttgtgtac cacacttatg
    14521 acgctgccag cgtttctgac ccaatggtta gctatactca aaaaattgta acagagccat
    14581 tcagacaaaa aagtgctgca caattaaatg agtttataga taaacttaat gctaaaaaca
    14641 aaagcagtat tgtaattgaa gcatttcata gctatgcaga tttaaaagat gcattgaatg
    14701 agttaattgc ctctaccggt gcacaattaa ttgtaatggg cattactggt ggtggagtat
    14761 ttaaagaaac ttttattggt agtaactctg taaatgttgc aaaaagtacc aatattcctg
    14821 taataatagt acctaccgaa acaagcttta gcccaattag taatatttta ttacttagcg
    14881 atttaaataa tgtagaagat actacaccaa cagaagctat taaaaatatt ttagatgcct
    14941 ctaaagcaaa attgcatgtt ttaaatgtta ccgataatac agaaaaagca gaagcttctt
    15001 ctgataaaca acagttagac aaattatttg aagcttataa ccctaatttt agctttatga
    15061 gtaacccaga ttttgtgggt gctgttgatt ggtttgttaa agaaaaagga attgacttgg
    15121 tgattgtaat acctaaaaaa catggtttat ttgaaacatt atttaaattt aaccacacta
    15181 aaacacttgc ctttcatgga aaagtgccat taatggctgt aaaaaaataa tctcataaaa
    15241 aaacctcttt ttaaagaggt ttttttgtat aggagtgttc aactactttt ttgctggtgg
    15301 cattaataca tcattaatta catgtatcat accattagat gttggtatag aagctactat
    15361 ttcagaacca tttacaaagg tctttccatc ttttttggta atagtaacat taccctcaaa
    15421 caccatattt aattcttggc catcttgcat catttctgct ttaatagagc ctacataagt
    15481 atggtagcct agagcgtttt ctaatttgcc tttattttct ggttttaata aatcttccac
    15541 tgtgcctgca ggtaatttat caaatgctgc attggttggt gcaaaaactg taaaagggcc
    15601 agcattgctt aaagcatcta ataagccagc tgctttaact gctgcaacta aggttgtatg
    15661 atctttactg cctgctgctg tttgtacaat attttttgct gattgatcgt caacaacacc
    15721 agattggcca actgcatctg tggtttgggt agtagattct gaacctgttg tagtttgctc
    15781 acctgaacaa gcagttaata aatacattgc cattgtggta agcacaataa acgttttttt
    15841 cataacaaaa atggtttagg gttgattgaa aatgcaaagt tgatatgaga aaaaacaata
    15901 aagcatgact gtaatcacac agaaggaaaa tattttgaat gttttaaagc tggtggttta
    15961 agtagttaat cagtaaactt ataaccaata cccctaacac tatgaaagtg tttagattgc
    16021 ttactatttt cttcgaaatg tttacgaaac attaatataa aattatctac cgttcttgta
    16081 gtaggaaaaa cattatagcc ccaaacaacc aataatattt tttctctagg tacaacttca
    16141 tttttatttt ctattaataa ctttaaaaga ttagtttctt ttttactaag cttaattttt
    16201 tttccatcat aggttattgc atctaacgat ttaaagtcaa ttaagttatt tccaaaagaa
    16261 taagtatcgc caagtgtgct ttctttatcg taaatttttt tatttttttg aattagcttt
    16321 tctaccctta ataataactc ttctaaatta aatggcttgg ttaaataatc atctgcccct
    16381 ttttttaaac ccaacactct atctgcccct gaatttttag cacttagcat taatatgggc
    16441 aactcatcat tacttaatct tactgtttgt gcaacagtta ttccatcaat ttctggtagc
    16501 ataatatcta aaatcattaa atcaaaatgg gctttgctta attcagttaa agcctcgtct
    16561 cctgcataag cacttgtaac atcataccct tccatttcta agtttaactt cagggtttcg
    16621 ttaaggcctt cttcatcttc taccaataat atgcatgctt tattctctat cataatttac
    16681 atattaaaaa cggctgtaaa aatacttcct tgtggatgat tgtttgtcat ggtaatgttt
    16741 ccattgtggc ttttcataat ttttttacat aagtataagc ctaagcctgt tcctttagtt
    16801 gttcttgtcg attcgctacc agatctataa aatttatcga atattttttt tctatcttgt
    16861 ggttttacgc cacaaccttt atctaataca tctaaaaatg tttgcttgtt gcttgtatat
    16921 aatctaatag ttacgggcat ttctttgggt gaatatttta atgcgttttc aattaaatta
    16981 tttacaagca tttgcaacaa caatgcttcg ccatttataa ataattgctg ttgtatatct
    17041 gcttctaatt ttcgttgagg gtatcttatg gcaaagtctt ctatgcaggt ttctgttagg
    17101 tcgcttaaat ttaattcgta tgtattactg taatatgctc ctccatctaa ttgagcagca
    17161 actaaaatat tattacataa actgtttaat ctatttgctt cctgaattgt gttagaaata
    17221 agtttctgtt gtttttcttc gtctaatttt cttttttgta gagtttctaa atttaaatga
    17281 gtaatggcaa taggtgtttt aagttcgtgt gtaaccgcca tcataaaatt ttgctgctga
    17341 accgatagtc ttatttgctt tctggttgcc ctatacacaa atactgcccc aattagtatt
    17401 actgctaaaa aagtagttcc ttctcccagg tattgtgcaa ttttcctttt tttagttgct
    17461 tctattgtag aaattttttc tttatataaa gaatcagatg tttgcaataa agcaatttgc
    17521 atttctgcag tatcgcttgc atgatggtta agtttaataa accaaaataa caatgcagca
    17581 atcatgtaca gtaataaaaa ccaatatact gtggtaatta aggctaattt tttatggaaa
    17641 aaagatttca ttatttagtt ctttctactc ttatccctgc atttaaaata ctatgcaatg
    17701 ggtcttctct cattatttga tgaagagtaa aaatatattt tcctttctta agtttagcgg
    17761 gtgccgtgct aaattttatt ctgtgttcta ttatatcact catagaggag ccaagccatt
    17821 tttcattatc tcctaataca aaatttcttt ttacagaaat agtggtatct ggtgattgca
    17881 tggatatatc cacccaaata ttattatact tatatgcatc ttcatgcctt agcacaaaat
    17941 ataagttata taaagtagta gtatcggaaa tttcaaaaac cgcttgaact ggttgctgac
    18001 ttttccattg atgattatta aaaaaagttg ttctttcata tacatctaac gttgtacacg
    18061 aagaaaaaaa tgccatcaca aaaaataaat acgcagtaaa tttcatttta aaacttgttg
    18121 aaataataaa tttctatcta aacaattttg ggttttataa tacatttaat acctgttcct
    18181 tcattttctc tagttcatct ttcattaata ctactttctt ttgtatatca gcattatttg
    18241 ctttgctgcc tgtagtatta atttctctgc caaattcttg taaaataaat gataattttt
    18301 tgcccttggc tttggttggt tcttctaaaa tatttctaaa ataattacaa tgatttatta
    18361 atcttacttg ttcttcgcta atatcaattt tttcaatata ataaatcaat tcttgttcta
    18421 atctgttagt atcataatta tttactccaa catattctat aagtagtttt tgtaatcctt
    18481 ctttaatttt ttctcttctt aaaggttcta acgttttaac ttcatcttgc aatttttcaa
    18541 tactaacaag gcgttgcagt aaatcacatt cagaactttt tccttcttct tttctatgct
    18601 cattaatgtc agataaaaca ttttttaata aggcttccac ttctttccat tcagtatcac
    18661 ttaaaatttc tgttgagttt gaaaccactt ctggtaatct taaaatagct gccatcaatt
    18721 gtgttttatc cgcattcaat tcttctgcta tttctgaaat gcttttatag tatgccttta
    18781 agagttcagt attaatactt acaggtttat taattccatt ttgtttaatt gtaataacac
    18841 attcaacact tcctcgttct aagtcttcgg caattttgct tcttatttca aactcaaagg
    18901 gttttaataa gttgggtaga aataacctta aatcaaattg ttttccgttt aatgatttta
    18961 tttcaattaa aaatgtttta tcgccaatta tcttttctcc tcttccataa cctgtcattg
    19021 aataaagcat aataaagtat tactgtttaa tgtaaagcaa ttttaaaagt atctcgtaaa
    19081 agtagtttaa aaaatttaat gcaaacatac aattcatcta ttgtactttt tgaggttaat
    19141 taattaaata aataaaaatt acgtgtttag tattattaat gcaatctatc ttgttaattt
    19201 taaccttcta aactagatta tgtatagcaa taaagagcaa tggctacaac agtcaaagtt
    19261 atttttaagt aatagtttaa atagccagtt tgttttatct aatattaaca ctcttagggc
    19321 tgttttgcag tttcatgaat ataattatta tgttttaaac aatgcattaa taagcgataa
    19381 tgaatacgat tttttgttta atttattaac aaaagcagaa aaagaaaatc cagagataat
    19441 taccaaagat tctcctacac aaagagttgg tagtagttta aacaatggct ttgaaacagt
    19501 acaacattta gtgccaatgc taagtttgga aaacagttat aacgaaacag atttactaga
    19561 ctttgataga aaggcaaaag aagcaagtgg tttacaagaa attgaatatt gcgtagagcc
    19621 taaattagat ggagctagta ttagtttaat ttatgaaaat gatttattaa cgagggctac
    19681 aactagaggc aatggtgtag agggtgatga tattacaacc aatataaaac aaattcgttc
    19741 cattcctcta tctgcacctt ttagtaaata tggtatcagg caaattgaaa taagaggtga
    19801 agtgatgatg accaaagagg catttcaaaa atataatgaa aagcttacgc agcaaggttt
    19861 agcaacagtt gcaaacccaa gaaacactgc tgctggaagt ttaagaatta aagatcctaa
    19921 agaagttgct caaagaaact tagatgcctt tttatatcat ataagttata ttactcaaaa
    19981 taatacccac aaggtaaata ctcattctca acagttaaac ttattatgga actgtgggtt
    20041 tcgttcaacc gaaaatgaaa aatttgttgc aaaaaatatt aatgaagtaa tagaatatgt
    20101 aacctccttt gaacaaaaaa gagacgattt gccttatgag attgatggaa tggttataaa
    20161 agttaatagc ttattattac aagagcagct tggtatgaca agccatcacc cacgctgggc
    20221 aattgcttat aaatttaaag caaggcaggc aacaactgtt ttaaatgcag tagagtttca
    20281 ggttgggcga acaggagcaa taacacctgt tgcaaaacta gagcctgtgt ttattggtgg
    20341 tgttacagtt tctagcattt cagtacacaa cgtagagtat attaaagaaa aagatttaaa
    20401 aattggagat accgttttaa tagaacgtgc aggtgatgtt attccacaaa ttgtaaaaag
    20461 cataaccaca gcaagaaaag gcaatgagca tgaaatagtt tttccagaaa attgccccgt
    20521 ttgtaaaaat caattattta aagaagaagg ggaagccgtt tggcgttgca ttaatattga
    20581 atgtgaagca caggtggtag aaaaaattat tcactttgcc agtaaagatg caatggatat
    20641 taaaggtctg ggagacgcta ttgtacgtaa gttttatgaa aaaaatttat taaaagacat
    20701 cccaactatc tacaagctaa acttaaccaa tattggcgaa atagaaggtt ttggcaaaaa
    20761 atctatagat aatttacaag ctgctattgc aagtagcaaa caacaaccat taaacagatt
    20821 aatttatggt ttaggaataa gatttgttgg cgaaacaaca gcaaaaactt tagccaacag
    20881 tgtagagcat ttgttagaat ttaaaaacaa aacaaccgac gatttacaaa cacttgaaga
    20941 tgtaggaaca aaagttgcta aaagtattca tcattttttt agtaatgaaa gtaatataaa
    21001 tatgattctt gaattagagc aattgggttt acaattaaaa aatactaaaa aacaaactat
    21061 taatattggc gaactggctg ggcaaacatt tttatttacc ggaaccctat ctaaactaaa
    21121 acgtaaccag gcagaggctt tggctgaaca acatggtggc ataattgtaa gtggtgttag
    21181 tgcaaaatta aattacttaa ttgttggtga cgacgcagga agtaaattag aaaaagcaaa
    21241 aaaaataaat actataaaga ttttaaatga agaagagttt ttgcagatga tagggtaaag
    21301 aatgattgat taggaaataa aatattcttt aaatgaagtt tcaaactcat caagtgtcag
    21361 cacatttact ttggggaaat ctattgtttt taatattttg aaatcagcat cattacttat
    21421 tataaaatct gcatttcctg ctattgcaca atctacaaat ttgttatcat ccaaatcagg
    21481 atatattgtt tgccaacgaa aagatggaga aattaacagc acgtttttat ttaataaaaa
    21541 ctctacaaac ttgtcgcttg aatattttac tccatattta atttgaaaaa gttcttcgta
    21601 ttcaagtaaa atttctgtac tgataagtaa attaaatttc ccattcagca aagcgtcata
    21661 tatccacctg taacgaaagt tttttgcaat ggaaacaaga aaaatatttg tatcaattac
    21721 taaattaaga tttttcattc agccattttt ctaaatcatt attggtaatg tttttttctt
    21781 ctatggcttt atttacatca tttgaaagtt tgctcaaaaa acgtttgccc aaaacatctt
    21841 ttaattcaat taattcttca tcgcttacat tatgagcaaa aaggtttagc aattccaact
    21901 gcacattgct gaattttgtt gatggcggta tttgaacatt aggagtattc attgtagtaa
    21961 tatttggtat aaaggtacag attttatttt tccctttacg taaacaaaaa ttgccttagg
    22021 caatagaata aacctgcaag gctataagct ttgctgagct gggtttttac aaaaaagcag
    22081 taataagcta atatttttat aaatttggaa tgtggaaagg aaaataaaaa tatttaaaac
    22141 atttgaagaa caagaagcat acactttgca gcagcagggc aaaaccactc cattagaacg
    22201 gtttagaaat cttttttata tgcaacaact ttctaagaaa tttaattctg taaaaaactc
    22261 aacaagaaaa attacagtac atcatggata ttt
//
LOCUS       DAOQPK010000009        21582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_1172_length_21582_cov_5.167278, whole genome shotgun sequence.
ACCESSION   DAOQPK010000009 DAOQPK010000000
VERSION     DAOQPK010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 21582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21582
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1172_length_21582_cov_5.167278"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..846
                     /locus_tag="PLU36_00915"
     CDS             133..846
                     /locus_tag="PLU36_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45340.1"
                     /translation="MEKKFLLILFSIFLNTVSAQKIIITGLVLDSIHNSPVEFASVTN
                     ISKNKTIICNNKGFFNMVVSFGDVLSVASVNHYFDTISVQKNILKDTIIFILKPITKN
                     LGEVTVETTINKYVYDSTQRRKQFLAKVGNGKIPVFSSANSGAGLGFNITHFSKSEKI
                     KRNAYKLFDLLEKEQYINYRFSPVVVTKYTQLQNEALLAFMQAHRPTYKWLRLNVTEE
                     DIKYYINEKLKLKKTTTNN"
     gene            932..2410
                     /locus_tag="PLU36_00920"
     CDS             932..2410
                     /locus_tag="PLU36_00920"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5106 domain-containing protein"
                     /protein_id="HMZ45341.1"
                     /translation="MKQLLIVTGLFFYLSANAQISKQATTNKTVQDKGYEIPITLSPY
                     KNTWIYLGSYFGKGKTLSDSAWLNEESKGVFKGKEKLTGGIYFIVSPSKNFIEFELLM
                     DNEQHFSITADTANRDKPTIKGSLENSLFFTYSKFSAEKGNHIQTLRGKYAAAKTKSD
                     STKCREEAIKEEKVLQAYREKIIKENPNSLLATLFHVMKKPEAPAIPIINGKADSLYP
                     YRFIKEHYWDDVNFYDDRILRTPFFEPKLDEYFKYYLSPEADSIIPEIKYMLLSARTG
                     KEMFPYLLIKFTNKYVKPEYMGQDKVFLYLFNEFYTKGDTIYLDAASRKMIFERAYSL
                     MANQIGEPAAPLNLTDTLGKTHSLYEIKSNYTLVVFWDPHCGHCKEQIPRIDSIYQAK
                     WKNMGLAVYSVYIYNDQLPDWKKFITEKKLFNWIHVYQTEEAKQYEAKNSIAGFRQLY
                     DARVTPTIYLLDDKKRIIAKQLTIEQFDDILTVKHKQIENKK"
     gene            2412..4343
                     /locus_tag="PLU36_00925"
     CDS             2412..4343
                     /locus_tag="PLU36_00925"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMZ45342.1"
                     /translation="MSKKFFLLITAACILQVLNAQNVFTYGKKAVSKAEFLKAFDKNP
                     TPGETDRKKALKEYLELYINYKLKVQSAYDDKLHEQPLFKNESSSFRKQLADNIINTE
                     ANIDNLIKEVFERSQKDIHVAQIFIEIKPGVDNIEAINQIEKAHKALKAGMDFEEAVK
                     KFSNDEYTIQTKGNLGFITTFTLPYQFENEIYKLKVGEFSSVYKSSFGYHIFKNLEER
                     SAAGKRKVAQILLAMPIGATKESKQYLATLADSIYHLAINGEDFEDLVEQYSNDRATA
                     SIGGVMQEFGIGEYAPNFEQPIFDLKNKGDIAKPFLTEHGWHIVKLLDIIPVSNNFSD
                     PVTVSNIKNQIERTDRMAFAKKALVKNWMNICKYKKEPFDEKDFMIYTDSALANINFT
                     GYKNIKPETVLFSFAKQKITVAEWIKFARAVKESKEKLAQLHTLVLLIEYENIRCSEY
                     YYDHLEDYYPTLKEQSKEFDEANLLFGAMDKHVWSKASEDTVGLKKYYAINKTKYVWQ
                     PGVSAIVVTAPTQALAVQVIDSFKAGLKDWKLVLNKINNNILFDSSRFENNQLPITQV
                     IENKIGFISNPEKNTNDNSYTFMYITALHDKIETKSFDEARGFVTNNYQQVLEQKWIQ
                     ALKKKYPIVINQAVWNTIK"
     gene            4396..6567
                     /locus_tag="PLU36_00930"
     CDS             4396..6567
                     /locus_tag="PLU36_00930"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HMZ45343.1"
                     /translation="MKKKYLLFVAAGLAICSTSALNAQKKESTKANMKKKSKVEQPST
                     NNNPLLKKSKLQYQAPEFDKIKDKDFKPAFDYGLNEQIKQIEAIANNTDKPTFENTIV
                     ALENSGQNLRRAITIFSNLNSANTNPFLQALDEEYAPIFAAHTDKIFLNEQLFQKIKR
                     LYATRANLKLDAEDVRLIEYYNQQFEIAGANLNNEQKEELKKINEQLATLQSQFNNKL
                     LAARKAGGLIIEDVKLLDGLSSNEIDAAAENAKVAGMPGKYLLALQNTTQQPLLQNLK
                     NRTVREKLFKASWLRAEKDDENDTRETLQQLARLRLKKAQIMGKKNFAEWKLQDQMAK
                     TPENAMNLLAKMAAPAVKKAKQEAEEIQKLIDKQNGGFKLEPWDWNFYAEQVRKAQYD
                     LDDNEIKPYFEVRTVLEKGVFYAAKELYGLTFKVRKDLPVYHPDVVAYEVFDKDGKSL
                     AIYYLDFYTRDNKNGGAWMSNFVEQSYSLGQKPVIVNVYNYQKPAPGKPSLISYDDVT
                     TLFHEFGHSIHGMFASQKYISISGTNTPRDFVEFPSQINEYWALEPSVLKNYALHYKT
                     GKPIPNELVKKIKNAKTFNEGYNVTEIVAAATLDMAWHTITDESEIIPANEFEKMALD
                     KFGLLVNEVPTRYHSPYFLHIWSNGYSAAYYAYLWSEMLDFNALEYFENNGGMKRENG
                     DRFRKYILSVGNSMDLNQAFRNFTGKDPEIEPLLRGRGLIK"
     gene            complement(6645..9185)
                     /locus_tag="PLU36_00935"
     CDS             complement(6645..9185)
                     /locus_tag="PLU36_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HMZ45344.1"
                     /translation="MKKIFFVVVVFASCNIVIAQKRTNPTSKDTTPTAAKTMPPQAAK
                     PKPGPKPFKEVITDKAISKKGLFNVHKVEDKYYFEIPNSLLGKEIMCVTRYSKVPGGA
                     GVYGGEVANQQTLKFEKGPNDNIFIRTITLISTADSSQKISTAVSNSHLDAIAAAFDI
                     KALGKDSTSSIIDVSDFFKGDNQIVSIDPNSKRRFSLSMLAGDRSYVQSINTYPINTE
                     IKTVKTYISSPMPSAGASQSSAAGFPAARDAGAVTIELNTSMILLPEKPMARRTWDKR
                     VGFFPDNFVVFSDDQQRVEDKTFAVRWRLEPKPEDVEKWKAGQLVEPIKPIIYYIDPA
                     TPPNWVPYLIAGINDWQKAFEKAGFKNAIMGKEWPKNDSTMSLEDARFSVIRYFASDI
                     ENAYGPNVHDPRTGEILESHIGWYHNVMKLVHDWYMVQTAAVNPRARKMVFDDDLMGQ
                     LIRFVSSHEVGHTIGLLHNMGSSSKTPVEKLRDKAWVEANGHTASIMDYARFNYVAQP
                     EDNINEMGLFPRIGVYDEWAIKWGYGYIPGNTEEEQNINSDKLIVKALAENPRHWFGT
                     YELGNSVDPRTQSEDLSDNVMIANEYGIKNLKRIIVNLPEWTKENGKGYDNLSEMYRQ
                     LVGQFNRYVNHVMRNVGGIYETFKSTSQQGDVFEFVPKATQKEAFNFINRNVLTTPTW
                     LLDKNILNKIQNPTNSESVQNIQISALNNLLDPSRLNRMIICANRYGNTNVYLFNDLM
                     DDTKKAVWSEAATGKAVDAYRRNLQKAHVERLINLLTFKPQAMVISGRTFATGPEIAT
                     TDIVSYTRGQLKALQAELTTASAATADRLTKFHYQDLVERIKLALNPR"
     gene            9536..10405
                     /locus_tag="PLU36_00940"
     CDS             9536..10405
                     /locus_tag="PLU36_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HMZ45345.1"
                     /translation="MADLKPTPTATKTISNVQPKKSSNVISWIAPIVCLILGYVIWRF
                     VFGSPNNFEKPDLNGWFWPDHHKLKPNTGMAKMYEGGIVVPILIGSFFIVITFVIERI
                     LTIQKATGAGNIGEFLRKVQFHLANKDVDKALAECDKQKGSVGNVMKSGLRTYKDMIS
                     NTELSTEQKVLNIQKSIEESTALELPMLEKNLVFLSTLASVATLLGLLGTVLGMIKSF
                     SALGEDSGGATAAAELSKGISEALFNTALGIGTSAVAIIMYNVFTTRIDAITFGIDES
                     GFTLTQSFASLYK"
     gene            10471..11115
                     /locus_tag="PLU36_00945"
     CDS             10471..11115
                     /locus_tag="PLU36_00945"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HMZ45346.1"
                     /translation="MGRAKIPRKSTNIDMTAMCDVAFLLLSFFILTTKFKPAEAVSVS
                     PPSSVSDKAAPESDIVLITIDKDGKVFLSMDNEEKKEYIANTIANEKGLKLDVQSFKK
                     AQFIGVPFNKLTSFLQLPDDQRKGDNMPGIPCKDSTNNEMIEWLRIVRSAYMGQKINL
                     LVKGDNTSKYPSFKNVITAFKKNDLLKFQIITNPEGVPVGSELYRQAQAGIKND"
     gene            11167..11724
                     /locus_tag="PLU36_00950"
     CDS             11167..11724
                     /locus_tag="PLU36_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HMZ45347.1"
                     /translation="MAEMDTSSGGSHKKGPGVKKSKKMSTRVDLTPMVDLGFLLITFF
                     IFTTTMSLPTTMNLFLPKDTDKPEEQNKIKDNSALTILLGNDNNVFYYEGMLDNANVS
                     ANFKSSNFGGGEDGIRNIILRKKANTDEKDFMVVIKPSDECTYKNVVDILDEMTINVV
                     KRYAMVDISDVEVQLIKATEAASSK"
     gene            11785..12621
                     /locus_tag="PLU36_00955"
     CDS             11785..12621
                     /locus_tag="PLU36_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HMZ45348.1"
                     /translation="MEKNNILTADILDIIFEGRNKAYGAYDLRKTYTKRITFAFVATV
                     TLCALFFVGSLLANSGEKKVAKILVQDVELESLKEPEEKKPEPPPPPPPPKQEPPKVE
                     MKQFTPPKIVKAEEVKPEEIPPEVTKLEEAAIGTKNQEGMKDVGVVAPVVEAVGTGKV
                     EAPKEEEDYNKIFTVVQIEAEFPGGKAAWNKYLQRNLNSSLPMDNGASPGKYTVVVTF
                     TVDKEGNVSDVQADNDPGYGTKDEAIKVIKKGPKWKPAVQNGRNVAYRHKQSITFIVS
                     EE"
     gene            12744..13487
                     /locus_tag="PLU36_00960"
     CDS             12744..13487
                     /locus_tag="PLU36_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HMZ45349.1"
                     /translation="MNYACTIVPVAPLRSEALHSSEMVSQLLFGECVTILDSTKDFFK
                     VSSMYDDYIGWCSKKQLTTVNENFSIQNHKQCTNIPITTALYKESNLYLSSGSFIGAL
                     KNNILTINDKIFNIHFNSTETNIQKVIHSYLNTPYLWGGKSIFGIDCSGFTQQVYKIL
                     GTKIYRDAYEQAQQGKVIGFLQEAAIGDLAFFDNENGKITHVGILINDHEIIHASGIV
                     RIDLIDNEGIINYETKERTHHLRLIKRIL"
     gene            13594..13926
                     /locus_tag="PLU36_00965"
     CDS             13594..13926
                     /locus_tag="PLU36_00965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HMZ45350.1"
                     /translation="MFSKSCEYAIKALIFLGQESNENKKVCVKTISSAIDAPQHFVAK
                     ILQDLTKKKLLKSTKGPNGGFYVDEENSKNSLADIIKAIDGTKIYSQCVLGLKECSEK
                     NPCPVHHE"
     gene            14053..16155
                     /locus_tag="PLU36_00970"
     CDS             14053..16155
                     /locus_tag="PLU36_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HMZ45351.1"
                     /translation="MNVKNKLKASILAVILLLVNKFATAQEHPNFINLDGKDIRSSVL
                     LFIIGCICIIIAIAYMQFKHAKEIGKNISALNKKNVLKDLNIEQLKTYINKSKSVGII
                     ILLLFSSLQSFAQTTTDKTKPLMSQPGIIITVLLIAIPILLAAWLIIARATKTVKKFN
                     NIKRLKQAEILASELREEANEESNKALKDRKTALEYSLQNIELGGSENATDDKGLILN
                     VGNTQEVHFTAVKRKAAPRPALEPAITKLVVWYLGCAAFWLVIGTGVGEYVGIKFIAP
                     DVDQVSWLSFGRLRPVHTNMVFWGWASLGMMGLGYYVVPMVSNTKLYSVKWGWIALVL
                     TNLQVTIGSISLMAGINNGGGEFREYIWPIMALLAVALILTVINFIKTIANRTTKEIY
                     ISNWYIVSSLMFTIIIALTAYLPFWQNGLGETIVQGYYMHTGVGMWFTLFTLGLLYYF
                     IPQQLNKPIYAYSLGILAFWTQILFYTIIGTHHFIFSSIPWWLQTVAIVGSVGMIIPV
                     FAATINYVMTFKGQWGKVSNSYTLPFFLVAVVYYFIVSMQGTAEAFRFTNLLWHFTDF
                     TVAHSHLGFYGIISFAIWGGAYAIIPRLTRKEPPQHLVGAHFWLAFIGLILYCFPLMF
                     GATLKGMMWMEGKPFIDSVVKMFDYWVWRAIGGSLMFFSHILFFYNFYKMSSKTIEDV
                     NVVDEAINILEQEPSTAK"
     gene            16164..17177
                     /locus_tag="PLU36_00975"
     CDS             16164..17177
                     /locus_tag="PLU36_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HMZ45352.1"
                     /translation="MNIFNNHTKLYTTAAIMYFVLTFFTAIKPALDNQQNNGPLPGTK
                     PLSGDALAGKMVYIAEGCVACHTQQVRNVDMDKMFGTRPGIPSDYANIGRTSVWQNTA
                     TLMGTERTGPDLTDVGNRQPSKEWNLLHLYNPRSVVAQSIMPSYKWLFEIKENPSKND
                     VIVNVPAEFMHGKTGKVVATKKALQLVAYLQSLKQTPLPESIKPYEFLYKQEKKVTAN
                     AGASEEETVDGKALFTANCQSCHQANGEGLKGAFPSLKGSPIVLGDDLKLIVDIIMNG
                     YDARPEFAVMTPVGTSMNFDEKQVAAIINYIKTSWGNDAKKTKADEVKEIIEFIKTNN
                     PAK"
     gene            17174..17434
                     /locus_tag="PLU36_00980"
     CDS             17174..17434
                     /locus_tag="PLU36_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45353.1"
                     /translation="MKKISFLLLLALLSAPAVYACPVCERNKPKILQGITPHGSMPNS
                     NWDYVIVIIAVLLLLISLFFSVKWLISPKEGNSDHIKFSIIK"
     gene            17443..17850
                     /locus_tag="PLU36_00985"
     CDS             17443..17850
                     /locus_tag="PLU36_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HMZ45354.1"
                     /translation="MQNQCSLKIFIDDNPKPIGEYAAPVSFEIDTSKLVDGEHYLRIV
                     SISNSGKEGIRTIPFIVRNGPAISIEGINENDVVDGVVPLMINAYSKGDQKKFLIEGS
                     ETPRSIPAWLWAIIILFVGWSMYYFITSALMKL"
     gene            complement(17912..18298)
                     /locus_tag="PLU36_00990"
     CDS             complement(17912..18298)
                     /locus_tag="PLU36_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HMZ45355.1"
                     /translation="MSKQIINTTNAPAPIGPYNQAVKTGNLLFVSGQIPINPATNELV
                     QGTIKEEATQVMLNLKAILSEANLSFEHIVKTTIFLSDMSLFAEVNEIYGSFFMKDFP
                     ARETVAVKGLPKGVNVEISVIATTVL"
     gene            18459..20450
                     /locus_tag="PLU36_00995"
     CDS             18459..20450
                     /locus_tag="PLU36_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HMZ45356.1"
                     /translation="MSKNLNKASATGFIIALGIIYGDIGTSPLYVFNAIISNKVVSEE
                     LIIGTLSCIIWTLTLLITIKYVFLVLKADNNGEGGIFSLYALVRRRKKWLIVPAMIGG
                     ATMLADGIITPPITVTPAIEGLKELPALREISTNTIVLIVLGILSIFFIMQQFGTASI
                     GKMFGPFMMIWFTMLAVLGIMHISDDFSILKAFNPMYAIKFLTHYPSGFWLLGAVFLC
                     TTGAEALYSDLGHCGKENIYVSWGYVKLCLVLNYLGQGAFLLSHYKGIEVNAATKASM
                     EINAFFDLMPQWFIIPGVIIATLAAIIASQAMISGSFTLISEAIRLNLWPKFKIRYPS
                     EERGQLFIPSVNLIMFVGCVTMVLHFRESSKMEAAYGLAIVLTMLSTTILFANYLVNK
                     RVQNSWIYLYLAIYIIIEASFLTALLQKFTHGGYITILLGGLMFAVMYVWFKSRKIKN
                     RYVEFVRLEDYISQIQELSNDKAIPKYATHLVYMTSSVHHKEIEHKIIYSILNKKPKR
                     ADIYWFVHVDVLDDPYTCEYIVEHIVPNDIIRIEFRLGFRMEQRINLMFKKVVTDLVA
                     NKEVNINSRYESLGKNNVVGDFQFIVLEKYLSQDNELPFFERIVMKLYFWLKEISLSE
                     ERGFGLDPGFVTVEKFPLIVAPVRNLNLKRVIPEQFDEE"
     gene            20565..21305
                     /locus_tag="PLU36_01000"
     CDS             20565..21305
                     /locus_tag="PLU36_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMZ45357.1"
                     /translation="MLVYFLQEKFIFKPEKLKADFKFKYDIPFTELFFNIEQGVCING
                     LHFYRTNPSGLILYFHGNTRSIKGWAKYARDFYRFNYDVVLVDYRGFGKSTGKRSEKE
                     MLADMQFIYDELKKKYPEHHLLVYGRSMGSGFATKIACDNKPRYLILDSPYYSFKKVV
                     ERFIPILPVKYILRYHLRTDKWIRKVNCHTYIIHGTKDYLIPISQSIKLQKLNPSKVT
                     LIKINGGGHNNLPNFDEYHNFIRDILKE"
BASE COUNT         7987 a         3135 c         3474 g         6986 t
ORIGIN      
        1 taataataaa aactaagcaa taaagttaag gataaatgaa ctatacaaaa gaaagttaaa
       61 agatacgggt aaagtaaata aagcggagcc aactgtactt ttaaccaact gtatttataa
      121 tatatttaca taatggaaaa aaaattcctc cttatactat tttctatttt tttaaatact
      181 gtttctgctc aaaaaataat tattacaggc ttagtgcttg atagtattca taacagccct
      241 gttgagtttg ccagtgtaac aaatatttca aaaaacaaaa ctataatttg caataacaaa
      301 ggctttttca atatggtagt tagctttggc gatgtacttt ctgtagcatc ggttaatcat
      361 tattttgata cgattagtgt ccaaaagaac attcttaaag acaccatcat ttttatatta
      421 aaacctatta caaaaaactt aggagaggta acagttgaaa caaccattaa taaatatgtt
      481 tatgatagca cacaaaggcg aaaacaattt ttagccaaag ttggcaatgg taaaatacct
      541 gtattttcaa gtgctaatag tggggcaggc ttgggtttta atattacaca tttcagcaaa
      601 tctgaaaaaa taaaacgcaa tgcctataag cttttcgatt tacttgaaaa agaacaatat
      661 attaattata ggtttagccc tgttgtagtt acaaaataca cccaattaca aaatgaagct
      721 ttattagcat ttatgcaagc ccataggcct acttataaat ggcttaggct aaatgtaacc
      781 gaggaggata taaaatatta tataaacgaa aaattaaagt tgaaaaaaac tactacaaat
      841 aactaggcat atattttgtt ataccatttt aaccttcttt acagtttgaa ttacttaaat
      901 ttacgcctcc aaataaagaa gtatcattaa aatgaagcaa ctacttattg taacaggttt
      961 atttttttat ctttcagcaa atgctcaaat ctcaaaacaa gcaactacca ataaaactgt
     1021 tcaggataaa gggtatgaaa ttccaattac actttcgccc tataaaaata cctggattta
     1081 tcttggtagt tattttggaa aaggtaaaac cttatcagat agtgcatggt taaatgaaga
     1141 aagcaaaggt gtatttaagg gcaaagaaaa acttaccgga ggcatttatt ttattgtatc
     1201 cccatcaaaa aattttattg aatttgaatt actgatggat aatgaacaac actttagcat
     1261 aacagccgat actgcaaata gagataagcc aactattaaa ggctctttag aaaattcgct
     1321 attttttaca tattcaaaat ttagtgcaga aaaaggaaac cacattcaaa cacttagagg
     1381 aaaatatgct gcagcaaaaa ctaaatcaga ctctacaaag tgcagagaag aagcaattaa
     1441 agaagaaaaa gtgttacaag cctacagaga aaaaattata aaagaaaatc ccaactcact
     1501 attggctact ttatttcatg taatgaaaaa accagaagca cctgctatac caattattaa
     1561 tggcaaagca gactctttat acccttatcg tttcattaaa gaacattatt gggatgatgt
     1621 aaatttttat gatgatagaa ttttaagaac tccttttttt gagccaaaat tagatgaata
     1681 ttttaagtac tacctaagcc cagaggcaga ttctatcatt cctgaaatta aatacatgtt
     1741 actttctgca agaactggta aagaaatgtt tccatattta ttaattaagt tcacaaataa
     1801 atatgtaaaa ccagagtaca tggggcagga taaggttttt ctttatttgt ttaacgaatt
     1861 ttatacaaaa ggcgatacca tttatttaga tgctgcatca agaaaaatga tttttgaaag
     1921 agcttatagt ttaatggcaa atcaaattgg agaacctgca gcacctttaa atttaacaga
     1981 tacattaggc aaaacccatt ctttgtacga aataaaatca aattacactt tagtagtttt
     2041 ttgggatcct cattgtgggc attgtaaaga acaaattcca cgaattgata gtatctatca
     2101 agcaaaatgg aaaaatatgg gattggctgt atattctgta tatatttata acgaccaatt
     2161 gcccgactgg aaaaagttta ttacagaaaa aaaattattt aactggattc acgtttatca
     2221 aacagaagaa gccaaacaat atgaagccaa aaacagtatt gcaggcttta ggcagctata
     2281 tgatgccaga gtaacaccaa caatatattt acttgatgat aaaaaaagaa ttattgccaa
     2341 gcaattaacc attgagcaat ttgatgatat attaactgtt aaacacaaac aaatagaaaa
     2401 taaaaaataa gatgagtaag aaattttttt tattaattac agcagcttgc attcttcagg
     2461 tacttaatgc acaaaacgta tttacttatg gaaaaaaagc agttagtaag gcagagtttt
     2521 taaaagcatt cgataaaaac cctacaccag gagaaacgga cagaaaaaaa gcattgaaag
     2581 aatatttgga actttacatt aattataaat taaaagttca gtctgcttac gatgataaat
     2641 tacatgaaca acccttgttt aaaaacgaaa gcagtagctt tagaaaacaa ttagcagata
     2701 atattattaa tacagaagca aatattgata atttaattaa agaagtattt gaacgtagcc
     2761 aaaaagatat tcatgtagct caaatattta ttgaaataaa accaggtgtt gataatatag
     2821 aagctataaa ccaaatagaa aaagctcaca aagcattaaa ggcaggaatg gattttgaag
     2881 aagctgttaa aaaattttct aacgatgaat atacaattca aacaaaaggc aacctaggtt
     2941 ttattaccac ttttacacta ccttatcaat ttgaaaatga aatttataaa ttaaaagttg
     3001 gagagttttc atctgtttat aaaagcagtt tcggctacca tatttttaaa aacttagaag
     3061 aaagatcagc agcaggaaaa agaaaagtag ctcaaatatt attagctatg ccaattggtg
     3121 caacaaaaga aagcaaacaa tatttagcta cactggcaga tagtatttat catttagcta
     3181 ttaatggaga agactttgaa gatttagtag aacaatatag caacgacagg gctacagcaa
     3241 gtattggagg ggtaatgcaa gaatttggaa ttggcgaata tgctccaaat tttgaacaac
     3301 ccatttttga tttaaaaaac aaaggagata ttgctaaacc attcttaaca gaacatggat
     3361 ggcatattgt aaaattgcta gatataatac cggttagtaa taatttttca gacccagtta
     3421 cagtttcaaa cattaaaaat caaatagaaa gaacagatag aatggctttt gctaaaaaag
     3481 cattagttaa aaactggatg aacatttgta aatataaaaa agaacctttt gacgaaaagg
     3541 attttatgat ttatacagat agtgccttag caaatatcaa ctttaccgga tacaaaaata
     3601 ttaaaccaga aactgtttta ttttcttttg ctaaacaaaa aattactgta gctgaatgga
     3661 taaaatttgc aagagcagta aaagaaagta aagaaaaact tgcacaacta cacacacttg
     3721 tattgctaat agaatatgaa aatattagat gtagcgaata ttattacgac catttagaag
     3781 attattatcc aacacttaaa gaacaaagca aagaatttga tgaagctaat ttactgtttg
     3841 gtgctatgga taaacatgta tggagcaagg ccagtgaaga tacagttggt ttaaaaaaat
     3901 attatgctat aaacaaaaca aaatatgtat ggcagccggg tgtttctgca attgttgtaa
     3961 cagcaccaac acaagccttg gcagttcaag ttatagacag ttttaaagct ggtttaaaag
     4021 attggaaatt ggttttaaat aaaataaaca ataatatttt atttgatagt agcaggtttg
     4081 aaaataacca acttccaata acacaagtta tagaaaacaa aattggcttt attagtaatc
     4141 cagaaaaaaa cacaaatgac aacagttata catttatgta tattactgca cttcatgata
     4201 aaatagaaac aaaaagtttt gacgaagcta gaggctttgt taccaacaac tatcaacaag
     4261 tgttagaaca aaaatggatt caggctttaa agaaaaaata ccccatagtt attaatcaag
     4321 ctgtttggaa tactattaaa taataaaata ttttacttca taaaatatat cttttaccat
     4381 ttaaactaat tgaccatgaa aaaaaaatat ctactctttg ttgcagcagg cctggcaatt
     4441 tgtagtacat cagcattaaa tgctcaaaaa aaagaaagta caaaagctaa catgaaaaaa
     4501 aaaagtaaag tagaacaacc cagtacaaat aacaacccct tattaaaaaa aagtaaacta
     4561 caataccaag caccagagtt tgataaaatt aaagacaaag attttaagcc tgcatttgat
     4621 tatggcttaa atgagcaaat aaaacaaata gaggcaattg caaacaatac agataaacca
     4681 acttttgaaa atacaattgt tgcactagaa aatagtggcc aaaatttacg tagggcaatt
     4741 accatttttt caaacttaaa tagtgctaat acaaatccct tcttacaagc tttagatgaa
     4801 gaatatgcac caatttttgc tgcacatacc gataaaatat ttttaaacga gcagcttttt
     4861 caaaaaataa aacggttata tgctaccaga gcaaacctaa aattagatgc agaagatgta
     4921 agattaattg aatattataa tcaacagttt gaaattgcag gagccaattt aaacaacgaa
     4981 caaaaagaag aattaaaaaa aataaacgaa caattagcta ccctgcaatc tcaatttaat
     5041 aataaattat tggctgctag aaaagcaggt ggtttaatta ttgaggatgt taaactttta
     5101 gacggcctct cttctaacga aatagatgca gctgccgaaa atgcaaaagt tgcaggtatg
     5161 ccaggtaaat atttattagc attacaaaat accacgcaac aaccgctttt acaaaattta
     5221 aaaaatagaa ctgtaagaga aaaattattt aaagcatcat ggttacgagc tgaaaaggac
     5281 gatgaaaacg acactcgaga aactttgcaa caattagcaa ggctacgttt aaaaaaggca
     5341 caaataatgg gtaaaaagaa ttttgcagaa tggaaattac aagaccaaat ggcaaaaacc
     5401 ccagaaaatg caatgaactt attagcaaaa atggctgccc ctgctgttaa aaaagcaaaa
     5461 caagaagcag aagaaattca aaaattaatt gacaaacaaa acggtggctt taaattagaa
     5521 ccttgggatt ggaattttta tgcagaacaa gtaagaaaag cacaatacga tttagatgat
     5581 aatgaaatta aaccttactt tgaagtaaga actgtactgg aaaaaggtgt tttttatgca
     5641 gctaaagaac tgtatggctt aacctttaaa gtacgtaaag atttacctgt ttatcatcca
     5701 gatgttgtgg cttatgaagt atttgataaa gatggaaaat cattagcaat ttattattta
     5761 gatttttata ctagagataa taaaaacgga ggagcatgga tgagtaattt tgttgaacaa
     5821 tcttacagtt taggacaaaa gcctgtaatt gtaaatgtgt acaattatca aaaaccagca
     5881 ccaggtaaac catctttaat ttcttacgat gatgtaacca ctttatttca tgagtttggc
     5941 catagtattc atggcatgtt tgcaagccaa aaatatattt ctatttctgg caccaatacc
     6001 ccaagagatt ttgtagagtt tccttctcaa attaatgaat attgggcatt agaaccatct
     6061 gtattaaaaa actatgcttt gcattataaa acaggaaaac caataccaaa tgaattagtt
     6121 aaaaaaataa aaaatgctaa aacatttaac gaaggatata atgtaaccga aattgttgct
     6181 gctgctactt tagatatggc ttggcataca attacagatg agagtgaaat tataccagca
     6241 aacgaatttg aaaaaatggc tttagataaa tttggcttat tggtaaatga agtacctacc
     6301 cgttatcatt ctccttattt tttgcatatt tggtctaacg gttactctgc agcttactat
     6361 gcatatttat ggagtgaaat gctagatttt aatgctctag aatattttga aaataatggt
     6421 ggaatgaaaa gagaaaatgg agacaggttt agaaaatata ttttatctgt tggtaactct
     6481 atggatttaa accaagcatt cagaaacttt acaggaaaag acccagaaat tgaaccttta
     6541 ttaagaggta ggggactaat aaaataacct gaatagtttt ttataaaaag aagaggttgc
     6601 aaaaatttgc aacctcttct tttttagatt ctatagcaag aaaactaacg aggatttaat
     6661 gctagtttta ttctttcaac caaatcttga tagtgaaact tagttaatct atctgcagta
     6721 gcagcacttg cagtagttaa ttctgcttgt aatgctttta attgaccacg tgtataagaa
     6781 acaatatctg ttgtggctat ttcagggcct gtagcaaaag ttctgccaga tataaccata
     6841 gcttgtggtt taaaggttaa taaattaatt aatctttcta catgggcttt ttgcaagttt
     6901 cttctataag catctacagc ttttccagtt gctgcttcgc tccaaactgc ctttttggta
     6961 tcatccatta aatcgttaaa taaataaaca tttgtattgc cataacgatt agcacaaata
     7021 atcattctgt ttaatcttga tggatctagt aaattgttta aagcactaat ttgaatgttt
     7081 tgtaccgatt cgctattggt tggattttgt attttattta aaatattttt atccaataac
     7141 caagttggtg tagttaatac atttctatta ataaaattaa atgcttcttt ttgtgttgct
     7201 ttaggaacaa attcaaaaac atctccttgt tggctagttg atttaaaggt ttcataaata
     7261 ccaccaacat ttctcattac atgatttacg tatctattaa attgaccaac taattggcga
     7321 tacatttcag ataaattatc ataccctttt ccgttttcct tagtccactc tggtaagttt
     7381 acaataattc tctttaaatt tttaatccca tattcgttag ctatcatcac attatcacta
     7441 agatcttccg attgtgtacg aggatctaca gagttgccca attcataagt accaaaccaa
     7501 tggcgtgggt tttctgctaa agctttcact attaatttat cgctattaat attctgttct
     7561 tcttctgtat taccaggtat ataaccatag ccccatttaa ttgcccattc atcgtacacc
     7621 ccaattcttg gaaataatcc catttcattg atattatctt ctggttgtgc aacatagtta
     7681 aaacgtgcat aatccattat agaagctgta tgtccattag cttctaccca agctttgtct
     7741 cttaattttt caacaggggt tttactactg ctacccatat tgtgtaataa accaattgta
     7801 tggccaactt cgtgagaaga aacaaaacga attaattgac ccattaaatc atcgtcaaaa
     7861 accatttttc ttgcacgtgg atttacagca gcagtttgta ccatatacca atcgtgtact
     7921 aatttcatta cattgtgata ccatccaata tggctttcta aaatttcacc agttcgtggg
     7981 tcgtgaacat tagggccata agcattttca atatctgatg caaaatatct aattacagaa
     8041 aaacgagcat cttctaaact catggtagaa tcgtttttag gccattcttt acccattatt
     8101 gcgtttttaa atccagcttt ttcaaaagct ttttgccaat cgttaatacc agcaattaaa
     8161 taaggcaccc aattaggagg cgttgcagga tctatgtaat aaataatagg ctttataggt
     8221 tctaccaatt ggccagcctt ccatttttcc acatcttctg gtttaggttc taacctccaa
     8281 cgaactgcaa aagttttatc ttcaactctt tgttggtcat cgctaaatac tacaaaatta
     8341 tctggaaaaa aacctactct tttatcccat gttcttcttg ccattggttt ttctggcaat
     8401 agaatcatag aagtatttaa ttctatagtt acagcaccag catctcttgc tgcaggaaag
     8461 ccagcagcac tggattggct tgcacctgcc gaaggcattg ggcttgaaat atatgttttt
     8521 acagttttaa tttctgtgtt aatgggatag gtatttatgc tttgtacata acttctatct
     8581 ccagcaagca tacttaagct aaatcttctt ttactatttg gatcaatact tacaatttgg
     8641 ttatctcctt taaaaaaatc acttacatca ataatactac tagtgctgtc ttttccaaga
     8701 gccttaatat caaaagcagc agcaatagca tctaaatgag agttactaac cgctgtagat
     8761 attttttggc tactatctgc agtactaatt aaagtaattg ttctaataaa aatattatcg
     8821 ttggggcctt tttcaaattt taaagtttgt tggttagcca cttcaccccc ataaacgcca
     8881 gctccaccag gtactttgct gtaacgggtt acacacataa tttctttgcc taataaacta
     8941 ttgggtattt caaaataata tttatcttct accttatgta cattaaataa ccctttttta
     9001 ctaatagcct tatcagtaat tacttcttta aaaggttttg ggccaggttt tggcttagct
     9061 gcttgcggag gcattgtctt agcagcagtt ggtgttgtgt ctttagaagt tggattggtt
     9121 cgtttttgag caataacaat attgcacgaa gcaaacacaa ctacaacaaa gaaaattttt
     9181 ttcataattg tttataaagt tggttaaaag aatggctaag ttaaggaagc gggtaatttt
     9241 ttaataaatt tcttgttttt tattacatca gcggttaaat aaactttatg aacagtattt
     9301 tatagtaact aatttatata gtattactaa ttaatagagt taaattttat ttcccattta
     9361 aaaaaaatca aataattttt ctaatgttta taattattaa tttaaaaaac tattaacttg
     9421 agcaactttt caatttcact tgaaataaaa tattaaaaag ctaatatttt ttcagtagaa
     9481 ttgctaaata tttattaatt taaaagtatt tttaaaaaac taaaacaatt gaattatggc
     9541 tgacttaaaa ccaactccaa cagcaacaaa aacaatttca aatgttcaac ccaaaaaaag
     9601 cagtaatgtt atttcatgga ttgccccaat tgtatgtctt attttaggat atgtaatttg
     9661 gaggtttgtt tttggatctc ctaacaattt cgaaaaacca gatttgaatg gttggttttg
     9721 gccagaccat cataaactta aaccaaatac aggtatggct aaaatgtatg aaggtggtat
     9781 agtagtacct atattaattg gttcgttctt tatagtaata acatttgtta ttgaaagaat
     9841 tttaactata caaaaagcta ctggtgcagg caatattggt gagtttttac gtaaagtgca
     9901 atttcattta gctaataaag atgttgataa agctttagca gaatgtgata aacaaaaagg
     9961 tagtgttggt aatgtaatga aatctggttt acgtacttat aaagatatga tttcaaatac
    10021 agaactatca accgaacaaa aggtactaaa tattcaaaaa tcaattgaag aatctacagc
    10081 attagaattg ccaatgctag agaaaaattt agtattccta tccactttag catctgtagc
    10141 aaccttatta ggtttattag gaacggtatt aggtatgatt aaatccttct ctgccttagg
    10201 agaagatagt ggtggtgcaa ctgcagctgc agaattatca aaaggtattt ctgaagcatt
    10261 atttaataca gcgttaggta ttggtacatc tgctgttgca attattatgt ataatgtatt
    10321 taccaccaga attgatgcta ttacttttgg aattgatgag tcaggcttca cattaactca
    10381 aagctttgca tctttataca aataattttt gtggaaagat gaatactttg aatctaaaca
    10441 attagtgaat aaccttaaaa tcaaaaaacg atgggacgtg caaagattcc taggaagagt
    10501 accaatatag atatgacagc aatgtgcgat gtagcttttt tgctcttatc gttctttatc
    10561 cttacaacaa aattcaaacc agccgaagca gtttctgtta gccctccaag ttctgtgtct
    10621 gataaagctg caccagagtc tgatattgtt ttaattacaa tagataaaga tggtaaagtt
    10681 tttttatcta tggataacga agaaaagaaa gaatacatag ccaatacaat tgccaacgaa
    10741 aaaggcttaa aattagatgt acaatcattt aaaaaagccc aatttattgg agtaccattt
    10801 aataaactaa cttcattttt acagctacca gatgatcaaa gaaaaggcga taatatgcct
    10861 ggaatacctt gtaaagacag taccaataac gagatgattg agtggcttag aattgtaaga
    10921 agtgcttata tggggcaaaa aataaattta ttagttaaag gcgataatac ctctaaatac
    10981 ccttctttta aaaatgtaat aacagcattt aagaaaaacg atttattaaa gtttcaaatc
    11041 attacaaacc cagagggagt gcctgtgggt agcgaacttt acagacaagc acaagccgga
    11101 attaaaaatg attaacttga atttgttctc ttaaattatt taaaaattaa ataaaaagta
    11161 accgaaatgg cagaaatgga tacctcgtcc ggtggcagtc ataaaaaagg ccccggcgtc
    11221 aaaaagagca aaaaaatgtc caccagggtt gatttaaccc ccatggtaga tttgggcttt
    11281 ttgttaatta cttttttcat atttactaca acaatgagtc tgcctactac tatgaatctt
    11341 tttttaccaa aagatacaga taagccagaa gagcagaata agattaaaga taatagtgca
    11401 ttaactattc tattaggcaa cgataataat gtgttttatt acgagggaat gttagacaat
    11461 gctaatgttt ctgcaaactt taaatcatcc aattttggag gcggagaaga tggtataaga
    11521 aatattattc ttcgcaaaaa agccaatacc gatgaaaaag attttatggt tgtaataaaa
    11581 ccaagtgatg aatgtactta taaaaatgta gtagatatat tagatgaaat gaccattaat
    11641 gttgtaaaaa gatatgcaat ggtagatata tctgatgtag aagtacaatt aataaaagct
    11701 accgaagctg catcatctaa ataattttta cttttcttat gtaaaattat ttagcactgc
    11761 atcttataat aaaattaatt agttatggaa aagaataaca ttttaacagc agacatactt
    11821 gatattatct tcgaaggaag aaataaagct tatggtgcat acgatttaag aaaaacttac
    11881 acaaagcgta taacttttgc ctttgttgca actgttacat tatgtgcctt gttttttgta
    11941 ggttcattat tagcaaattc aggagaaaag aaggtagcta aaattttggt tcaagatgtt
    12001 gaattagaaa gcttaaaaga gccagaagag aaaaaacctg aaccaccacc accacctcct
    12061 cctcctaaac aagagcctcc taaggttgag atgaaacaat ttactcctcc aaaaattgta
    12121 aaagcagaag aagtaaaacc agaagaaatt ccaccagaag ttactaaact agaagaggct
    12181 gcaattggta ctaaaaacca agaaggtatg aaagatgttg gtgttgttgc ccctgttgtt
    12241 gaagcagtag gtactggtaa agtagaagca cctaaagaag aagaagatta taataaaata
    12301 tttacagttg ttcaaattga ggctgaattt cctggaggta aagctgcttg gaacaaatat
    12361 ttacaacgca atttaaatag cagcctacca atggataatg gtgcaagccc tggaaaatat
    12421 acagttgttg ttacttttac tgtagataaa gaaggtaatg taagcgatgt gcaagcagat
    12481 aatgaccctg gttatggcac aaaagacgaa gcaataaaag taattaaaaa aggacctaaa
    12541 tggaaaccag cagtacaaaa tggtagaaac gttgcttatc gccacaaaca aagtatcaca
    12601 tttatagttt ctgaagagta attataactt tcataaacaa ttttagaggt tgcttgtaag
    12661 cagcctcttt tttttgtaat tgctaaaaaa tatttccatg tttaaaaata ttcaactgta
    12721 ttttagtaaa taaatttata gcaatgaatt atgcttgtac aattgtacct gttgcaccat
    12781 taaggagtga ggcattacat agcagtgaaa tggttagtca attattattt ggagaatgtg
    12841 taacaatttt agattcaaca aaagattttt ttaaagtttc atccatgtat gatgattata
    12901 ttggctggtg ttctaaaaaa caactaacaa cagttaatga aaatttttct attcaaaacc
    12961 ataagcaatg taccaatatc ccaattacaa ctgctttata taaggagagt aatttgtatt
    13021 tatcgtctgg ctcttttatt ggtgcattaa aaaataatat tttaaccatc aacgataaaa
    13081 tttttaatat tcattttaat tcaaccgaaa ctaatattca aaaagtaatt cattcttacc
    13141 tcaacactcc atacttgtgg ggtggtaaaa gtatttttgg tatagattgt agtggcttta
    13201 cccagcaagt atataaaata ctaggaacaa aaatttacag agatgcttat gagcaagcac
    13261 aacaaggaaa ggtaattggc tttttgcaag aagctgccat aggcgacctt gctttttttg
    13321 ataatgaaaa tggaaaaatt acacatgtag gtattttgat aaacgaccat gaaattattc
    13381 atgcatctgg tattgttaga atagatttaa tagataatga aggaattatt aattatgaaa
    13441 ctaaagaaag aacccaccat ttaaggctaa taaaaagaat tttatagaaa aaatttgtac
    13501 attttatcaa aaatttcgtt gaataaaaaa atcagataaa aaactctttt tatatgaatc
    13561 aattgtcata cttttgtcta taattctgct tatatgtttt caaaatcatg tgaatatgca
    13621 attaaagctt tgattttctt gggccaagag tctaatgaaa ataaaaaggt ttgtgtaaaa
    13681 acaatttcaa gtgcaataga tgctccacaa cattttgttg ccaaaatatt acaagaccta
    13741 acaaagaaaa agttattaaa atcaacaaag ggccccaatg gtggttttta tgtagatgaa
    13801 gaaaattcta aaaattcttt agcagatatt attaaagcta tagatggcac taaaatatat
    13861 agccaatgtg tgttaggctt aaaagagtgt tcagaaaaaa acccttgccc agtacatcat
    13921 gaataaaaac attaaattaa acttaattaa aatgattgaa aataatacca tttcaacttt
    13981 taatacacag agtaatttag gtaaatattt tttaaaaaat gattaatata aaagaataaa
    14041 aggatattaa atatgaatgt aaaaaacaaa ttaaaagcca gcatattagc agtaatttta
    14101 ttactggtta ataaatttgc aaccgcccaa gagcatccta attttattaa tttagatgga
    14161 aaagacatac gaagtagcgt attgttgttt ataattggat gcatttgtat aatcattgca
    14221 attgcttata tgcagtttaa acatgctaaa gaaattggaa aaaacatttc ggctttaaac
    14281 aaaaaaaatg ttctaaaaga tttgaatata gaacagttaa aaacctacat caacaaatcc
    14341 aaatctgtag gtatcattat tctactgcta tttagctctc ttcagagttt tgcacaaact
    14401 actacagaca aaacaaaacc attaatgtct caaccaggca ttattattac tgtgctatta
    14461 atagcaatac caatactatt ggcagcatgg ttaattatag caagagcaac taaaacagtt
    14521 aaaaaattca ataacataaa aagattaaaa caagccgaaa ttttggcatc agaattaaga
    14581 gaagaagcta atgaagaatc taacaaagcc cttaaagacc gaaaaacagc tttagaatat
    14641 tcattacaaa atattgaatt aggaggaagt gaaaatgcaa cggatgataa aggattaata
    14701 ttaaatgtag gaaacacgca agaagtacat tttactgcag taaaaagaaa agcagcacca
    14761 aggcctgcat tagaacctgc aattacaaaa ttagtagtat ggtatttagg ttgtgctgca
    14821 ttttggttag taattggtac aggtgttggc gaatatgttg gtataaaatt tattgcaccc
    14881 gatgttgatc aagtaagttg gctaagcttt ggaaggttaa ggccagtgca caccaatatg
    14941 gtattttggg gttgggcttc tttaggtatg atgggtttag gttattatgt tgtaccaatg
    15001 gttagcaaca caaaactata tagtgttaaa tggggttgga ttgcacttgt attaaccaat
    15061 cttcaagtta ccattggctc cataagttta atggcaggta ttaacaatgg tggaggtgaa
    15121 tttagagaat atatatggcc tataatggca ttgcttgcag ttgctttaat acttactgta
    15181 attaatttta taaaaaccat tgctaataga actactaaag aaatttatat atctaactgg
    15241 tatattgttt cttccttaat gtttaccatt atcattgcat taactgctta tttacccttt
    15301 tggcaaaatg gtttaggaga aaccattgta caaggatatt atatgcatac aggcgttggt
    15361 atgtggttta ctttgtttac actaggctta ttatattact ttataccaca acaattaaat
    15421 aagccaatat atgcttatag cttaggcatc ttagcatttt ggacacaaat tttattttat
    15481 acaattattg gtacacacca tttcattttt agttctattc cttggtggtt acaaacagta
    15541 gctattgttg gtagtgtagg aatgataatt cctgtttttg cagcaacaat caactatgta
    15601 atgacattta aaggccaatg gggtaaagta tctaatagtt ataccttacc atttttttta
    15661 gtagcagtag tttattactt tatagtttct atgcaaggca ctgcagaggc ttttagattt
    15721 accaatttgc tttggcattt tactgacttt actgtagcac actcacatct tggtttttat
    15781 ggaattattt cttttgctat ttggggaggt gcttatgcaa ttataccacg tttaacaaga
    15841 aaagagccac cacaacattt agtaggtgcc catttttggt tagcatttat tggtttaata
    15901 ttatactgtt ttccgttaat gtttggagca actttaaaag gaatgatgtg gatggaagga
    15961 aagccattta tagattcggt agttaaaatg tttgactatt gggtttggag agcaattggc
    16021 ggaagcctta tgtttttctc tcatattctt ttcttctata acttttataa aatgtcttca
    16081 aaaactatag aagatgtaaa tgttgtagat gaggcaatta atattttaga acaagaacct
    16141 tcaacagcta aataatttaa aagatgaata tatttaacaa ccataccaaa ttatatacta
    16201 cagcggcaat catgtacttt gtattaactt tttttaccgc tataaaacct gctttagaca
    16261 atcaacaaaa caatggccca ttgccaggta ctaaacctct tagtggcgat gcattagctg
    16321 gtaaaatggt ttatattgct gaaggttgtg tagcatgtca tacacaacag gtaagaaatg
    16381 tagatatgga taaaatgttt ggaaccagac caggaattcc atcagattat gccaatattg
    16441 gaagaacaag tgtttggcaa aatactgcca ctttaatggg cacggaaaga acaggaccag
    16501 atttaacaga tgtaggtaat agacaaccaa gtaaagagtg gaatttatta cacctttata
    16561 accctcgtag tgttgttgca caatcaataa tgccaagtta taaatggctt ttcgaaataa
    16621 aagaaaatcc ttctaaaaat gatgttatag taaacgtacc tgcagagttt atgcatggta
    16681 aaacaggcaa agtggttgca accaaaaaag ctttacaatt agttgcttat ttacaatcat
    16741 taaaacaaac cccattacca gaaagtataa aaccttatga gtttttatac aagcaagaaa
    16801 agaaagtaac tgccaatgca ggtgcttcag aagaagaaac agttgatggt aaagcattat
    16861 ttactgctaa ctgccaaagc tgtcatcagg caaatggcga gggtttaaaa ggtgcatttc
    16921 cttcattaaa aggtagccca attgtattgg gagatgattt gaaactaatt gtagatatta
    16981 ttatgaatgg ttatgatgct cgtcctgaat ttgcagtaat gacaccagtt ggaacaagta
    17041 tgaactttga tgaaaaacaa gtagctgcaa ttattaacta tattaaaacc agttggggca
    17101 acgatgctaa aaaaacaaaa gcagatgagg tgaaagaaat aattgaattt attaaaacca
    17161 ataatcctgc aaaatgaaaa aaataagctt tctcctatta ttggctttac tttctgcacc
    17221 tgctgtatat gcttgtccag tgtgcgaaag aaataaacca aaaattttac aaggcattac
    17281 accacatggc agtatgccaa acagcaattg ggattatgta attgtaataa tagctgtttt
    17341 acttttattg ataagtttat ttttttctgt taaatggctt ataagcccta aagaaggaaa
    17401 ttctgaccat attaaatttt caattattaa ataaaaataa ttatgcaaaa tcagtgctca
    17461 ttaaaaatat ttatagacga taatccaaaa ccaattggtg aatatgcagc tcctgtatct
    17521 tttgaaatag atacaagtaa attagtggat ggagagcatt atttaagaat tgtaagtata
    17581 agtaactcag gcaaagaagg cattagaacc ataccattta tagtaagaaa tggacctgca
    17641 atatctattg aaggcattaa tgaaaatgat gtagtagatg gtgttgtacc tttaatgata
    17701 aatgcctaca gcaaaggaga tcagaaaaaa ttcttaatag aaggaagtga aaccccacgt
    17761 agtattcctg catggttgtg ggcaataatt atcctatttg ttggatggtc tatgtattac
    17821 tttattacct ctgctttaat gaagctataa tatattcatt gctgccttca tcaatataac
    17881 acctatgaga tgatgttggc agcaattttt tttaaagaac tgtagtagct attacactaa
    17941 tttctacatt cactcctttg ggtaaccctt taactgcaac agtttccctt gctggaaaat
    18001 ctttcataaa aaagctacca tatatttcat ttacttcagc aaataaactc atatcgctaa
    18061 gaaaaatagt tgttttcaca atatgctcaa agcttaagtt ggcttcagat aaaatggctt
    18121 ttaagttcaa cataacctgt gtagcttctt ctttaattgt tccttgcacc aattcattag
    18181 tggcaggatt aataggaatt tgaccactta caaatagcaa gttaccagtt tttactgctt
    18241 ggttataagg gccaataggt gcaggtgcat tggttgtatt aataatttgt tttgacatac
    18301 gtttacattt atagaggggc aagtaaatca ttttttgtaa aaccacaaag cctaatgcag
    18361 aaaataaaaa aatatcatcc tattaaagaa ataaattaaa aatagttctt gtatattttt
    18421 ataaattata catttgttct atcatttaaa aaagatttgt gtcaaaaaat ttaaacaaag
    18481 cctccgctac aggttttatt attgctttag gaattattta tggcgatatt ggtacttctc
    18541 ctctctatgt ttttaatgct attataagca acaaagtagt ttcagaagag ttgattattg
    18601 gtacactatc ttgtataatt tggacactaa cactacttat tacaatcaaa tatgtttttc
    18661 ttgttttaaa agcagataat aatggcgaag gaggtatttt ttctttatat gctttagtaa
    18721 gaaggcgaaa aaaatggtta attgtacctg caatgattgg tggtgcaacc atgcttgcag
    18781 atggtattat cacaccacct ataacagtaa ctcctgccat agaaggactt aaagaattac
    18841 cagcccttag agaaatatca accaatacta ttgtattaat agtacttgga attttatcga
    18901 tattttttat tatgcaacaa tttggtactg caagtattgg taaaatgttt ggaccattta
    18961 tgatgatatg gtttaccatg ttggcagttt tgggaataat gcatatatca gatgatttta
    19021 gtattctaaa agcatttaac ccaatgtatg ctataaaatt tcttacacat tatccaagtg
    19081 gtttttggtt attgggtgct gtatttttat gtaccacagg tgccgaagcc ctatacagcg
    19141 atttaggaca ttgtggcaaa gaaaatattt atgtatcctg gggctatgta aaactttgtt
    19201 tagtattaaa ttatttaggg caaggggcat ttttattatc tcattataaa ggtatagaag
    19261 taaatgctgc tactaaagcc tctatggaaa taaatgcatt ttttgattta atgcctcaat
    19321 ggtttattat tccaggtgta ataattgcta cacttgcagc cattattgca agccaggcaa
    19381 tgatttctgg ctcatttact ttaataagtg aagctattag attaaatctt tggcctaagt
    19441 ttaaaattcg atacccatct gaagaaagag gacaactttt tataccaagc gtaaacctta
    19501 ttatgtttgt tggctgtgtt actatggtat tacactttag agaatcttct aaaatggaag
    19561 cagcttatgg tttagcaatt gtattaacca tgctttctac aaccatttta tttgctaatt
    19621 atttagtaaa caaaagagtt caaaacagtt ggatttattt gtatttagca atctatatta
    19681 ttatagaagc ttctttctta actgctttat tacaaaaatt tactcatggt ggttatatta
    19741 caatactact aggtggttta atgtttgccg ttatgtatgt ttggtttaaa agcagaaaaa
    19801 taaaaaaccg ctatgttgag tttgttagac tggaagatta tatttctcaa atacaagaac
    19861 taagcaacga taaagctatt ccaaaatatg ctacccactt agtgtacatg accagttctg
    19921 ttcatcataa agaaattgaa cataaaatta tttattccat acttaataaa aaacctaaac
    19981 gagcggatat atattggttt gtacatgtag atgttttaga cgatccttat acctgtgaat
    20041 atattgtaga acatatagta ccaaacgata taataagaat tgaatttagg ttaggtttta
    20101 gaatggagca acgtataaac ctaatgttta aaaaggttgt tacagattta gttgccaata
    20161 aagaagtaaa tataaatagc cgatacgaaa gcttaggaaa gaataatgtt gttggagatt
    20221 ttcagttcat tgtacttgaa aaatatttaa gccaggataa tgaattacct ttctttgaaa
    20281 gaattgtgat gaaactctat ttctggttaa aagaaatttc attaagcgaa gaaagaggct
    20341 ttggcttaga tccaggtttt gtaacagtag aaaaatttcc attaattgtt gcacccgtac
    20401 gaaatttaaa tttaaaaaga gttattccag aacagtttga tgaagagtag aaataaataa
    20461 gcatttgctt aaaatctaac caaatatttt ctatatttat tgtataattt ttttctatca
    20521 atcagtgggt attatatata atttgtagcc taatagtgtt atctgtgctg gtatattttt
    20581 tacaagaaaa atttatattt aaaccagaaa aattaaaagc cgattttaag tttaaatacg
    20641 atataccatt taccgaactt ttttttaata tagaacaagg tgtatgtata aatgggctac
    20701 atttttatag aactaaccca tcaggtctta tcttatattt tcatggcaat accagaagta
    20761 taaaaggttg ggcaaaatat gcaagagatt tttacagatt caattacgat gttgtattag
    20821 tggattatag aggatttgga aaaagtacag gaaaaagaag cgaaaaagaa atgttggcag
    20881 atatgcaatt tatttatgac gaactcaaaa aaaaatatcc cgaacatcat ttattagtgt
    20941 atggaagaag tatgggtagt ggttttgcta caaaaattgc ctgcgataat aaaccacgtt
    21001 atttaatttt agattcgcct tattatagtt ttaaaaaagt agtagaaaga tttattccaa
    21061 tattgcctgt taaatacatt ttaaggtatc atttaagaac agataaatgg ataagaaaag
    21121 taaactgtca tacctatatc attcatggta caaaagatta tttaatacct atttctcaaa
    21181 gtataaaatt gcaaaaacta aatccatcaa aagttacatt aataaaaata aatggtggtg
    21241 ggcataataa tttaccaaat tttgatgagt atcataattt tataagagat attttaaaag
    21301 aataacactt tagtttagca gattcttctt tacacccaag aacgaataag cgttttctat
    21361 gatgttagat aagaattact attgataaca aaagtaatga ttttttgtac taatttattt
    21421 tgttcataca tttgactctc tttggggcat aaaatttact tcgattttta aaataaacta
    21481 ttatgaaatt tacttagcta atatgcttta tttatttttt aaattttact tttcaaacca
    21541 tacaagcaca atgtaaccat aatttatgga gtggttcaac tt
//
LOCUS       DAOQPK010000010        21142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_1217_length_21142_cov_4.298667, whole genome shotgun sequence.
ACCESSION   DAOQPK010000010 DAOQPK010000000
VERSION     DAOQPK010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 21142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21142
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1217_length_21142_cov_4.298667"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="PLU36_01005"
     CDS             <1..695
                     /locus_tag="PLU36_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HMZ45358.1"
                     /translation="FREAGAKVVAIAEYNGAIYNANGLNEEECFEYKKKNGSLLGFPG
                     ATDIPNSNDALELECDILIPAALENVINKNNADRVKAKMIGEAANGPLTPEADDVFNA
                     KGILVIPDMYLNAGGVTVSYFEWLKNLSHVRYGRMEKRFDENQNEKIIRQIESLTGKA
                     ISDAERHQIVHGADEVDLVQSGLEETMITATQEIMEIWKANPEIPDMRTAAYVNAINK
                     VATSYSELGIFP"
     gene            complement(758..1069)
                     /locus_tag="PLU36_01010"
     CDS             complement(758..1069)
                     /locus_tag="PLU36_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ45359.1"
                     /translation="MDRIEQIRTFLQDNPKDNFLRHALALELIKIGKDSEAKTLFEAI
                     LNEYPDYVGSYYHLAKLFERNQQIETAKEWYLKGMEVSKKAKDQHSYNELQAAYENLV
                     Y"
     gene            complement(1077..1589)
                     /locus_tag="PLU36_01015"
     CDS             complement(1077..1589)
                     /locus_tag="PLU36_01015"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HMZ45360.1"
                     /translation="MRNFHNYIRRLILTVVDFFYFPFKRIMPLQTYRYAACGGFNTLL
                     GIVLYFVAFHYIFIHETVHITEQFALKRHIASDFLFSFWINFPIGFYLNRYVVFQESK
                     LPKRIQLFRYFVVLCGSIGINYFCLKLFVEIFKIYGPIAKILTAIIVIAFSYISQRNY
                     SFKTTKEGVY"
     gene            1651..3426
                     /gene="argS"
                     /locus_tag="PLU36_01020"
     CDS             1651..3426
                     /gene="argS"
                     /locus_tag="PLU36_01020"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HMZ45361.1"
                     /translation="MQIIENIKYTITTAIKELFDLELAPNEVLINKTKQEFTGDYTIV
                     LFGLVKQLKQNPEDIGNKIGNFLKENNSDLFLDFNVIKGFLNVTIQDAIWAGFLNENA
                     NNSNYGIATKTNHTVMVEYSSPNTNKPLHLGHLRNIFLGWSIAEIFKTLGNKVIKTCI
                     VNDRGIHICKSMIAWQLFANGDTPEKSKLKGDHFVGKYYVKFNEELKKQTEALLAQGV
                     SKENAEKETPIMKATQQMLLDWEDGNEAVLTLWKTMNNWVYSGFDITYKNIGSDFDKI
                     YYESDTYLLGKNLVETGLSKNVFYKKEDGSVWIDLQNEGLDEKLVQRKDGTSVYITQD
                     IGLAAQRYTEYEAQESIYVIGDEQNYHMKVLKLICQKLQLPGSNGMHHLSYGMVELPE
                     GKMKSREGTVVDADDLVEEMINISKQKTEELGKVSDFSSVELDELNKVIGLGALKFFL
                     LRVDPKKKMIFDPVESIDFHGFTAPFIQYTHARIKSILRKNSITNNASSNSKLLPLEK
                     EVVRHLELFNATLLEAAKEHDPSKIAIYVFNLAQVFNSFYAQHSIAHAENENQKLLRL
                     QIASLTAHVIKKGMHVLGIEVPERM"
     gene            3605..5446
                     /locus_tag="PLU36_01025"
     CDS             3605..5446
                     /locus_tag="PLU36_01025"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HMZ45362.1"
                     /translation="MAYASLEECLIDLEQHHHLIKINEEVDPYLEMAAIHLRVHEMNG
                     PALLFNNVKGTKFRAASNLFGNIERSKFIFRDTFKTVQELIEIKNNPINVLKKPFKNI
                     KAGIAALKAFPLKNPIHKPVLYQEINISDLPHIHHWKKDGGAFITLPQVYTEDIEQPG
                     VMHSNLGMYRIQLTGNDYVLNKEIGLHYQLHRGIGIHQTKANKKGCSLKVSCFVGGPP
                     AHTLSAVMPLPEGMSELTFAGVLGARRFRYIYQNEFCISTDADFVITGEVIVNENKPE
                     GPFGDHLGYYSLTHDFPVLKVNKVYAKKNAIWPFTVVGRPPQEDTSFGALIHELTGSA
                     LKNEISGVKEIHAVDAAGVHPLQLAIGSERYTPYLPSKQPAELLTIANHILGTGQLSL
                     LKFLFITADDTNNLSTKNIQSFFTYVFERIDYRRDIHFYTNTTIDTLDYSGTSLNTGS
                     KVVFAAYGDVIRNLCKEVPSCFYGLQNFANPQIVIPGVIALKAKTFTTYENAKQEMEQ
                     LNSYLYSYSSELKDVAQIIVCDDSNFLSEKLNNYLWVTYTRCNPSHDIYGVAEFIEYK
                     HWGCTSSVILDARIKPHHAPLLEKDEATERKIDRLFEKGGSLYSLIK"
     gene            5462..5998
                     /locus_tag="PLU36_01030"
     CDS             5462..5998
                     /locus_tag="PLU36_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HMZ45363.1"
                     /translation="MNWKKVAVFCGSKLGNNQLYYQHAVELGKLLAEHKIQMVYGGGK
                     VGIMGAIADAVMEYNGTVTGIIPELLVEWEQQHENISQLHVVKDMHIRKKMMYELCDA
                     VIVLPGGYGTMDELFEVITWNNLKIHEKKTILLNTAGYYNHLIAHIDTMAKEGFLYNN
                     WNENIIVANNPAAIFSSF"
     gene            complement(5979..6611)
                     /locus_tag="PLU36_01035"
     CDS             complement(5979..6611)
                     /locus_tag="PLU36_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45364.1"
                     /translation="MKKIIVCFGWLLLVLNSNAQRGEVKELGFKKQNVFIGGSLTLGY
                     GNVSNSYGGGSNFVIGANPEIGYSLAEWIDVGTVFNIIYSSTRFNDYTYRYRQTGLNT
                     GVGAFIRLYPIPSFFLLAQPEHNWIKVTQTNLDQPGVPKITQKVQASSFLVGVGYGQR
                     IIGSSSFFTTIMFDLGKEIYSPYRDGFNNAIPIVRTGFNWYLNSAKKKKK"
     gene            complement(6645..8048)
                     /locus_tag="PLU36_01040"
     CDS             complement(6645..8048)
                     /locus_tag="PLU36_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HMZ45365.1"
                     /translation="MATISTDQIKFLIALIGADNVLYDTESLEKYSRDETEKLKFYPD
                     VIVKPKTTNEVAAIMQFCNQHLIPVTPRGAGTGLSGGALATLGGVILSTERMNSIIEI
                     DTRNMFVITEPGVITEDLMNAVKEKNLFYPPDPSSKGSCFIGGNIAENSGGPKAVKYG
                     VVKDYVLNVELVLPTGEVIWTGANVIKNATGYNLTQLIVGSEGTLGIVTKIVLKLLPL
                     PKVDLLMLVPFKNLNNASDAVSAIFNAGFTPSALELIEIEALKITSKLIGNHSVPVSD
                     DIAAHLIIETDGNDKEVLMKEMETIAELMQQYNCEEIFFAEDANQKQELWKLRRRAAE
                     AVKLEGYTIEEDTVVPRAELTKLIVGVKELGKQHGFKVVCYGHAGDGNLHIRINHPSI
                     KNSYGSEEMINILKKLFELVKSLGGTISAEHGIGLIQKPYMDIFFNKTNLQIMRSIKK
                     VFDPNNILNANKIFDIN"
     gene            complement(8152..8240)
                     /locus_tag="PLU36_01045"
     tRNA            complement(8152..8240)
                     /locus_tag="PLU36_01045"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8204..8206),aa:Ser,seq:gct)
     gene            complement(8408..12694)
                     /gene="rpoC"
                     /locus_tag="PLU36_01050"
     CDS             complement(8408..12694)
                     /gene="rpoC"
                     /locus_tag="PLU36_01050"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HMZ45366.1"
                     /translation="MAIKKDVNKTKPGFSKITISLASPDSILERSFGEVLKPETINYR
                     TYKPERDGLFCERIFGPVKDYECACGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHI
                     KLVVPVVHIWYFKSLPNKIGYLLGMSSKKLETIVYYERYVVIQGGVKESLGTGDLLTE
                     EEYLDIIDTLPKENQYLPDEDPEKFIAKMGAEAVHDLLERLDLDELSYQLRNAAANET
                     SQQRKADALKRLSVVEAFRDSSARISNRPEWMVMQYIPVIPPELRPLVPLDGGRFASS
                     DLNDLYRRVIIRNNRLKRLMEIKAPEVILRNEKRMLQEAIDSLFDNSRKSNAVKAEGG
                     RALKSLSDVLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKMHECGLPKDMAAELFKP
                     FIIRKLLERGIVKTVKSAKKYVDKKEAIVWDILENILKGHPVLLNRAPTLHRLSIQSF
                     QPRLIEGKAIQLHPLVTAAFNADFDGDQMAVHVPLSSAAILEAQLLMLSSHNILNPQN
                     GTPITLPSQDMVLGLYYITKIKKSTKEEPVKGEGKTFYSLDEVVIAYNENVVDLHAGI
                     KVKTTVRENGQLVNKLIDTTVGRVLFNQHVPKEVGYVNALLTKKSLREIIGEIIKITN
                     VPKTAKFLDDIKTLGFRMAFKGGLSFSVNDLIVPEERNELLENAKAEVDEVWESYNMG
                     LITNNERYNQVVDIWGRADMIITETLIHKMTTDKQGFNSVFMMLDSGARGSKTQVKQL
                     VGIRGLMAKPRKSGSSGSEVIENPILSNFKGGLNVLDYFISTHGARKGLADTALKTAD
                     AGYLTRRLVDVAQDVVISEEDCGTLRGIAISALKDNEDIIEPLADRIEGRTSLHDVLH
                     PVTDALIIKAGEEISSEVAKEIENSGIETVEIRSVLTCESKRGVCVKCYGKNLATGYI
                     TQSGDAVGIIAAQSIGEPGTQLTLRTFHVGGVAGSSSVESSLNAKFDGTVQFDGLRTV
                     SSVNNEGNKVNIVIGRTGEVRTMDVKNDRLLITNNVPYGSTLLVKDGQQIKKGDVICT
                     WDPFNNVIVAEQNGTVEFESLIDGITFREESDDQTGHREKVIIETKDKTKIPTVIVSG
                     KDNKHYNLPVGSHLAVDEGEEVKAGQVLVKIPRILGKLRDITGGLPRVTELFEARNPG
                     NPAVVCEIDGIVTFGNVKRGNREIAVESKDGMIKKYLVPLSRQILVQDGDFIKAGAPL
                     SDGQIAPADILAIKGPFAVQEYVVNEIQEVYRLQGVKINDKHIEVIVRQMMKKVEIVD
                     AGDTKFLEEDLEDRVDFIAENDRIFDKKVVVDAGESTKMRAGKIITLRELREENSILR
                     RADKKTVEVRDAKPATATPVLLGITKASLGVQSWISAASFQETTKVLSTAAINGKVDY
                     MTGLKENVITGHLIPAGTGQREFDNLIVGSKEEYELLTAAREAMSFDDDE"
     gene            complement(13033..13317)
                     /locus_tag="PLU36_01055"
     CDS             complement(13033..13317)
                     /locus_tag="PLU36_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45367.1"
                     /translation="MYKYLTFTILTSVLCITASAGEFNPKPNKSIANKNVTATIQTTK
                     KPLGFIKKTNTLAALKLNYQEVVKVKIDSNTVVEYVPIFVEKKIKIKRNS"
     gene            complement(13606..14802)
                     /locus_tag="PLU36_01060"
     CDS             complement(13606..14802)
                     /locus_tag="PLU36_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45368.1"
                     /translation="MVPNIRNNFNEHFYQSNYESLIEDINTKFPNALDFRVAETPVFV
                     DKEFTQKMIDCCENIVDVITGFNFKTLTSHGIPANVRVANENNHSHFIAFDFGVCENN
                     EGKLEPQLIEMQGFPTLFAYQVLLDQVTRKYFSIPDNYTPYLSNLNEESYLNLLKEII
                     VGNHPAENVILLEIFPHQQKTRVDFYCTEAYLGIKTICLTELILEGKKLYYEKDGKKI
                     EVKRIYNRLIFDDLQQQTAEVQEKGKILLSDLEVEWVPHPNWFYRISKYTLPYINHPF
                     VPETKFLTDLIELPTDLENFVLKPLFSFAGQGVIIDVTKEDISKVKDPENWILQKKVN
                     YADIIKTPDGPAKAEVRIFYFWKDGEARPIATFNLARISKGKMIGVRYNKGKEWVGGH
                     IAYFEK"
     gene            complement(14886..15722)
                     /locus_tag="PLU36_01065"
     CDS             complement(14886..15722)
                     /locus_tag="PLU36_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase"
                     /protein_id="HMZ45369.1"
                     /translation="MNWAESKKIKIAILDLYEGHANQGMRCIREIINTWAEHNQLEVN
                     FDEFDLRLKEEIPDTSYHIYISTGGPGSPLETIGTNWEQKYFKWLQSIEEWNNNATNI
                     PKHVFFICHSFQLICRHYKIGNVCKRKSTSFGVFPVHKLEEGNEEMVFENLKEPFYVV
                     DSRDYQVIEPNYSILREMGAKILAIEKNRPHVPFERAIMAVRFNDYFIGTQFHPEADA
                     IGMKLHLLTEEKKATVILNHGEEKWRSMVEQLGDPEKILWTYNHILPNFLNHAVGVLT
                     EA"
     gene            complement(15832..16515)
                     /gene="scpB"
                     /locus_tag="PLU36_01070"
     CDS             complement(15832..16515)
                     /gene="scpB"
                     /locus_tag="PLU36_01070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HMZ45370.1"
                     /translation="MELSAIIPHIEVLIFASEKPLTALEIVELINNSFGFLEERINLD
                     QVESCIEGIVEKYNTEFYPFEVKESGGGFQFLTKKDFHQTVAQLNGDKFLKRLSTAAL
                     ETLAIIAYKQPITKGEIESLRGVNSDYSVQKLLEKELIVIAGRNENLPGHPLLYTTSK
                     SFMDYFGINSTDDLPKIKEILSEQLVEGTIINTDDFTSNTIIDEVETNLPNTENSNNS
                     ISENSNTEQ"
     gene            complement(16544..17428)
                     /gene="atpG"
                     /locus_tag="PLU36_01075"
     CDS             complement(16544..17428)
                     /gene="atpG"
                     /locus_tag="PLU36_01075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HMZ45371.1"
                     /translation="MAGQLKEVRNRIKSVQSTQQITKAMKMVSAAKLRRAQDAITQMR
                     PYAQKLQEMLSNIVSNIDGDTELKLAENRPVEKVLLIVVTSDRGLCGAYNSNIIKLAK
                     STIDQKYSTQYQKGNVAIWNIGKKGWEALTKAGYKTSDTFKDIFQQLSFTNVQAAAQA
                     ATKAFENNEFDAVEIIYSHFKNAATQTFIAEPFLPIPKVESNTNINKKSDFIFEPNKD
                     ELIAELMPKILNTQLYKAVLDGNASEHGARMTAMDKASDNANELLKTLKISYNRARQA
                     AITTELTEIVGGAAALQG"
     gene            complement(17565..18521)
                     /locus_tag="PLU36_01080"
     CDS             complement(17565..18521)
                     /locus_tag="PLU36_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HMZ45372.1"
                     /translation="MLTNTNKLAIGIDIGGTSTKFGIVNSKGEILEQNKISSTQHADI
                     NLFIAELYQVINPLIEKYGGKNAIKGIGIGAPNGNFYTGTIEYAPNLIWKEIIPLASL
                     IQKAFNLPTFLTNDANAAAFGEMKYGKAKEIKHFITITLGTGVGSGIVIDGNIVLGHD
                     GFAGELGHTIIRHNGRYHKGTGAYGSLEAYASANGIKQTALELLSSTNNSSLLRNYQP
                     EEITSEIIYNCAIQEDKIAKQVFEFTGEILGEALANFVMFSSPEMVVLFGGIAKAGDL
                     LLIPTQKAMEKNLLPIFKNKVKLVLSNLHEADAAILGASALV"
     gene            complement(18554..19021)
                     /gene="rnhA"
                     /locus_tag="PLU36_01085"
     CDS             complement(18554..19021)
                     /gene="rnhA"
                     /locus_tag="PLU36_01085"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HMZ45373.1"
                     /translation="MHLLKIYTDGASRGNPGKGGFGIVMLWGNTKKEIAAGFRLTTNN
                     RMELMAVIVALKQLTKKNISVTIYTDSKYVVDSIEKKWLDNWIKTDFKGGKKNKDLWL
                     EFYTLSKDFKIKMQWVKGHAENVFNNRCDELATTAADANNLLIDEGYEKTAGG"
     gene            complement(19039..20667)
                     /locus_tag="PLU36_01090"
     CDS             complement(19039..20667)
                     /locus_tag="PLU36_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HMZ45374.1"
                     /translation="MNVELNRNEDWMKLKLSEVKQHLSKIKLGGGKKAIDKQHEKNKL
                     TARERIEYLCDKDKPFIELGSFAGFEMYAEEGGCPAGGTVAGIGYICNRQCIIVANDQ
                     TVKAGAWFPITGKKNLRMQEIADENNLPIIYLVDSAGVYLPMQDEIFPDKEHFGRIFR
                     NNAKMSANGIIQIAAVMGACVAGGAYLPIMSDETLMVENNGSIFLAGSYLVKAAIGED
                     IDNETLGGAITHTEISGIADYKFKTEQECLQQIKKIISKISHQPTAGFDRINAIEPTK
                     NSQEIYSIMPEGNAKPYDMREIIERMVDASEFDEFKKDYGKTILCGYARIEGWAVGIV
                     ANQRLIVKSKKGEMQMGGVIYNDSADKAARFIMNCNQKKIPLIFLQDVTGFMVGSRSE
                     HSGIIKDGAKLVNAVANSVVPKITIIVGNSYGAGNYAMCGKAYDPRFIYAWPSAKIAV
                     MGGEQAAKTMLQIQVATMKAKGKELTQEEETELLDKLKSKYNNQTTPYYAAARLWVDE
                     IIDPLQTRQLISEAIHAANHQNIIPEFKTGVFQV"
     gene            complement(20834..>21142)
                     /locus_tag="PLU36_01095"
     CDS             complement(20834..>21142)
                     /locus_tag="PLU36_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HMZ45375.1"
                     /translation="TTLVVINSVCGCAAGACRPGVIMAVNNATTKPDNLATSFAGYDT
                     EAVQKVREYLMPYPPSSPAIALFKDGNLVSMFERHQIEGYPAQAIAQKLITEFEKYCN
                     "
BASE COUNT         6910 a         3488 c         3150 g         7594 t
ORIGIN      
        1 attttagaga agcaggtgca aaagtagttg ctattgcaga atataatgga gccatttata
       61 atgccaatgg cttaaacgaa gaagagtgtt ttgaatataa aaagaaaaat ggttctttat
      121 taggctttcc tggtgcaaca gatataccta atagtaatga tgctttagaa ttagaatgtg
      181 atatattaat tccggctgct ttagaaaatg ttataaataa aaataatgca gatagagtaa
      241 aagctaaaat gattggcgaa gctgcaaatg gcccattaac tccagaagct gacgatgttt
      301 ttaatgcaaa aggaatttta gtaattccag atatgtattt aaatgcaggc ggtgtaaccg
      361 tttcgtattt tgaatggtta aaaaatctta gccacgttcg ttatggcaga atggagaaaa
      421 gatttgatga aaatcaaaat gaaaaaatta tcagacaaat tgaatcgtta acaggcaagg
      481 caattagtga tgcagaaaga catcaaattg tacatggtgc agatgaagtt gatttagtac
      541 aaagtggatt agaagaaaca atgataaccg caacacaaga aattatggaa atttggaaag
      601 ccaatcctga aattccagat atgcgtacag cagcttatgt aaatgcaatt aataaagttg
      661 ctacgagcta ctccgaacta ggaatttttc cttagtacat ttaagaatta ttgttataaa
      721 attaaacccc gatatttttc ggggtttaat tttatggtta atacactaag ttttcgtatg
      781 ctgcttgtag ttcgttataa ctatgctgat cttttgcttt tttagatact tccattcctt
      841 ttaaatacca ttcttttgct gtttcaattt gctggtttct ttcaaataat ttagctaaat
      901 gataatagct gcctacataa tctggatact catttaagat tgcttcaaat aaagttttcg
      961 cttcagaatc tttaccaatt tttattaatt ctaaagctaa tgcatggcgt aaaaaattat
     1021 ctttagggtt gtcttgcaaa aaggttctta tttgttcaat tctatccata taatttttaa
     1081 tatactcctt ctttagtagt tttaaatgaa taattacgtt gagaaatata gctaaaagca
     1141 attacaataa ttgctgttaa aattttagct attgggccat aaattttaaa aatttctaca
     1201 aataatttta aacaaaagta gttaattcct atagagccac ataacaccac aaaatatcta
     1261 aaaagctgta tcctcttagg taatttagac tcttgaaaaa caacatatct atttaaataa
     1321 aagcctatag gaaaattaat ccaaaatgaa aataaaaaat ctgaggctat atgccttttt
     1381 aaagcaaatt gttcggtaat atgaactgtt tcatgaataa aaatataatg aaaagcaaca
     1441 aagtacaaaa ctatacccaa caaagtatta aagcccccac aagctgcata tctataagtt
     1501 tgtaaaggca ttattctttt gaaaggaaaa taaaaaaaat caaccactgt taaaataagc
     1561 cttctaatat agttatgaaa attacgcata aaacaaggct gctaagatag tgtgtaaatt
     1621 attagctttg cacccaaaag tttaagtggt atgcaaataa tagaaaacat aaaatataca
     1681 attacaacag ctataaaaga attatttgat ttggaattag cacctaatga agtattgata
     1741 aataaaacca aacaagagtt tactggagat tacactattg ttttatttgg gttagttaaa
     1801 cagcttaaac aaaatccgga agatatagga aacaaaatag gtaatttttt aaaagaaaat
     1861 aatagcgatt tattcttaga ttttaatgta ataaaaggtt ttttaaatgt aaccattcaa
     1921 gatgcaatat gggcaggttt tttaaatgaa aatgcaaata attctaacta tggaattgct
     1981 accaaaacta accatacagt aatggtagag tattcttctc ccaatacaaa taaacctttg
     2041 catttagggc atttaagaaa tattttttta ggttggagta ttgcagaaat ttttaaaaca
     2101 cttggtaaca aagttattaa aacttgtata gtgaatgata gaggaataca tatttgtaaa
     2161 agtatgatag cctggcaatt atttgcaaat ggagataccc cagaaaaaag taaattaaag
     2221 ggagaccatt ttgttggtaa gtattatgta aagtttaatg aagaattaaa aaaacaaacc
     2281 gaagctttac ttgcacaagg agtaagtaaa gaaaatgcag agaaagaaac tccaattatg
     2341 aaagcaaccc aacaaatgtt acttgattgg gaagatggaa atgaagctgt attaacctta
     2401 tggaaaacaa tgaataattg ggtttatagt ggtttcgaca ttacatataa aaatattggt
     2461 agcgattttg ataaaattta ttatgaaagt gatacttatt tattaggtaa aaacttagta
     2521 gaaactggcc tttctaaaaa tgtattttat aaaaaagaag atggtagtgt ttggattgat
     2581 ttacaaaatg aagggttaga tgaaaaatta gtacaaagaa aggatggaac ctctgtttat
     2641 attacacaag atattggttt agctgctcag cgttataccg agtatgaagc acaagagagt
     2701 atttatgtaa taggagatga gcagaactac cacatgaaag ttttaaaatt aatttgccag
     2761 aaattacaat tgccaggttc taatggtatg catcatttaa gttatggcat ggttgaatta
     2821 ccggaaggta aaatgaaaag ccgtgaagga acagttgtgg atgctgatga tttagtagaa
     2881 gaaatgataa acatatcaaa acaaaaaaca gaagaattag ggaaggtaag cgattttagt
     2941 agtgtagaat tagatgaact taataaagta attgggctag gtgcattaaa attctttcta
     3001 cttagagtgg accccaaaaa gaaaatgatt tttgacccag tagaaagtat agattttcat
     3061 gggtttactg caccttttat acaatataca catgccagaa taaaaagtat tttaagaaaa
     3121 aattcaataa cgaataatgc aagtagtaat tcaaaattac taccactaga aaaagaagtt
     3181 gttaggcatt tagaactttt taatgcaaca ttattagaag ctgctaaaga acatgaccca
     3241 agtaaaattg ctatttatgt ttttaattta gcacaggtat ttaatagctt ttatgcacaa
     3301 cattccatag ctcatgctga aaatgaaaac caaaaactat taagattgca aattgcatct
     3361 ttaacggcac atgtaattaa aaaaggtatg catgttttag gtattgaagt tccagaaaga
     3421 atgtaaaaaa tattctatta ggtaaagcaa tagttaagta ataaaactaa atttacttcc
     3481 aacttataaa atacgctaat cctatttttt ataattgaat gcagttaact atttagtttc
     3541 tctgttcttt taattctgaa ttaaaatata ctttactttt attgcataaa caaaaaactg
     3601 tagcatggct tacgctagtt tagaagagtg tttgattgat ttagaacaac atcatcattt
     3661 aataaaaata aatgaagagg tggatcctta tttagaaatg gctgccatac atttaagagt
     3721 acacgaaatg aatggccctg cattactttt taataatgta aaaggaacaa aattcagagc
     3781 tgcatcaaac ctatttggta atattgaaag gagtaagttt atatttagag atacatttaa
     3841 aacagttcaa gaactaatag aaattaaaaa caatccaata aatgttttaa agaaaccttt
     3901 taaaaatatt aaagcaggaa ttgcagcctt aaaagcattt ccattaaaaa atcctattca
     3961 caaaccagtt ttataccaag aaattaatat tagcgattta ccacatattc atcattggaa
     4021 aaaagatgga ggagcattta ttacattacc tcaagtttat accgaggaca tagaacagcc
     4081 aggtgtaatg cactctaacc ttggtatgta tcgtattcaa cttactggta atgattatgt
     4141 gttgaataaa gaaattggtt tgcactatca attacatcgt ggcattggta ttcaccaaac
     4201 caaagcaaat aaaaaaggtt gctcgttaaa agttagttgt tttgttggtg ggcctcctgc
     4261 tcatacttta agtgctgtaa tgccattgcc agaaggtatg agtgaattaa cttttgcagg
     4321 agttttaggt gcaagaagat ttaggtacat ttatcaaaat gaattttgta taagtaccga
     4381 tgcagatttt gtaattactg gtgaagttat agttaatgaa aataaacccg aaggcccttt
     4441 tggagatcat ttaggttatt attcattaac acatgatttt ccggttttaa aagtcaataa
     4501 agtttacgct aaaaaaaatg caatatggcc ttttacagta gttggcaggc caccacaaga
     4561 agatactagt tttggggctt taatacatga gcttacaggt agtgcattaa aaaatgaaat
     4621 tagtggtgta aaagaaattc atgctgtgga tgctgctggt gtacatcctt tacaattagc
     4681 tataggtagc gaaagatata cgccttattt gccttccaaa caaccagcag aattattaac
     4741 aattgccaat catattttag gtacagggca actaagttta ttaaagtttt tatttattac
     4801 agccgatgat accaataatc tttctacaaa aaatattcaa tcctttttta cctatgtttt
     4861 tgaaagaatt gattatagga gagatattca tttttatacc aatactacaa tagatacttt
     4921 ggattatagt ggtacaagct taaatacagg cagcaaagtt gtatttgcag cctatggcga
     4981 tgtaattaga aatttgtgta aggaagttcc tagttgtttt tatgggcttc aaaactttgc
     5041 taatccacaa attgtaattc ctggagttat tgctttaaaa gcaaaaactt ttacaaccta
     5101 tgaaaatgct aaacaagaaa tggagcagtt aaattcatat ctatattctt acagcagtga
     5161 gttaaaagat gttgcacaaa taattgtttg tgatgacagt aatttcttat cagaaaaatt
     5221 gaataattat ttatgggtaa cttacacacg ttgtaatcca agtcatgata tttacggagt
     5281 tgctgagttt attgagtata aacattgggg atgtacaagt tctgtaattt tggatgcaag
     5341 aattaaacca catcatgcac cgttattaga aaaagacgaa gctacagaaa ggaaaattga
     5401 cagattattt gaaaagggtg gaagtttata tagtttaata aaataaaaaa ttaggttgtt
     5461 tatgaattgg aaaaaggttg ctgttttttg tggaagtaaa ttaggtaata atcaattata
     5521 ttatcaacat gcagtagagc ttggcaaatt actagcagag cacaaaatac agatggttta
     5581 tggtggaggt aaagtaggaa taatgggtgc cattgctgat gcagtaatgg agtataatgg
     5641 aactgttact ggcatcattc ctgagctttt ggtagagtgg gagcaacagc atgaaaatat
     5701 cagtcaattg catgtggtaa aagacatgca tattagaaag aaaatgatgt acgagttatg
     5761 cgatgctgta attgttctgc caggtggcta tggtactatg gatgagttgt ttgaagtaat
     5821 tacctggaac aatttaaaaa tacatgaaaa gaaaacaata ttgcttaata ctgcaggcta
     5881 ttacaaccat ttaattgcac atattgatac aatggcaaaa gaaggttttt tgtataataa
     5941 ttggaatgaa aatattattg ttgctaacaa tcctgcagct attttttctt ctttttagca
     6001 gagtttaaat accagttaaa tcctgtacga acaataggaa tagcattatt aaaaccatct
     6061 ctataaggag aataaatttc ttttcctaaa tcaaacataa tagtggtaaa aaaactggaa
     6121 ctgcctatta ttcgttgtcc ataacctacg ccaaccaaaa aacttgatgc ttgtactttt
     6181 tgagtaattt taggaacacc aggttgatct aaattagttt gagttacttt tatccagtta
     6241 tgttcaggct gtgctaatag aaaaaaacta ggaataggat aaagccttat aaaggcacca
     6301 actcctgtat ttaagcctgt ttgcctatat cggtaagtat aatcattaaa tctagtactt
     6361 gaataaataa tattaaaaac agtacccaca tctatccatt ctgctaaaga gtaaccaatt
     6421 tctgggttag caccaattac aaaattacta cctcctccgt atgagttact tacatttcca
     6481 tagcctaaag taagactacc accaataaaa acattctgtt ttttaaaacc tagttctttt
     6541 acttctcctc tttgtgcatt actatttaaa acaagtagca accaaccaaa acatacaatt
     6601 atttttttca tgagaattta gcttaacaat tcaaaataaa tatattaatt aatatcaaaa
     6661 attttatttg cgtttaaaat attattaggg tcaaaaactt ttttaatact acgcattatt
     6721 tgcaaattgg ttttgttaaa aaaaatgtcc atataaggtt tttgaattaa ccctattcca
     6781 tgttcggcac taatagtacc acctaagctt tttactaatt caaaaagttt ttttaaaata
     6841 tttatcattt cttcactacc ataactgttt ttaatgcttg ggtgattaat tcttatatgt
     6901 aaatttccat cccctgcatg gccgtaacaa actactttaa aaccatgttg tttacccaat
     6961 tctttaactc caacaattaa ttttgtaagt tctgctcttg gcacaactgt atcttcttct
     7021 attgtgtaac cttctaattt tacagcttca gcagctcttc ttctcaattt ccacagttct
     7081 tgtttttgat tagcatcttc tgcaaaaaat atttcttcgc aattgtattg ttgcattaat
     7141 tctgcaatgg tttccatttc tttcattaaa acttctttat cattaccatc ggtttcaata
     7201 attaaatgtg ctgcaatatc atctgaaaca ggtactgaat gattaccaat taatttgctc
     7261 gtaattttca atgcttcaat ttcaataagt tctaatgcac ttggtgtaaa acctgcatta
     7321 aaaatggcac tcactgcatc gcttgcatta tttaagttct taaatggtac aagcattaat
     7381 aaatctacct taggtaatgg caatagtttt aaaacaattt ttgttacaat acctaatgtg
     7441 ccttcgctgc ctacaattaa ttgtgttaag ttatatcctg ttgcattttt aataacattg
     7501 gctcctgtcc aaattacttc accagttggc aaaacaagct ctacatttaa aacataatct
     7561 tttacaacac cgtatttaac tgcttttggg ccgccactat tttctgcaat attgccacca
     7621 ataaaacaac tacctttact gctagggtct ggaggataaa ataaattttt ctctttaaca
     7681 gcattcatta agtcttcagt aattacccca ggttcggtta taacaaacat atttctagta
     7741 tctatttcaa taatactatt cattctttct gtacttaaaa tcacacctcc taaagtagcc
     7801 aatgcacctc cacttaaacc tgtgccagca ccacgtggag ttactggaat aagatgctga
     7861 ttacaaaact gcatgattgc agcaacctcg tttgttgttt ttggttttac aataacatct
     7921 gggtaaaatt ttaatttctc tgtttcatct ctactatatt tttctaaaga ttctgtatca
     7981 tataaaacat tatcagcacc tattaatgct ataagaaatt ttatttgatc tgttgatatt
     8041 gtagccataa tatttttcta aagcaaatgt atgttataag aattatagat aataaatatt
     8101 gcattaggtt taaagaataa aaaaagagac tgataaaaat caatctcttt gcggtgagag
     8161 agggattcga accctcggta cagtttgacc cgtacggcag tttagcaaac tgctgatttc
     8221 agccactcat ccatctcacc aacattatcc ctattctgct ataacgaaag ggctgcaaat
     8281 ataatagcaa attgtatata gaaaaaacta tttgtaaata gtttttttat ttttttttaa
     8341 agaatttgga ataaaaaatg caagcttttt aaggcctgca tttttatatt gaaagaataa
     8401 ttttaaacta ttcatcatca tcaaaagaca ttgcctctct tgctgcagtt aataattcat
     8461 attcttcttt actacctaca attaaattat caaattcacg ctgcccggta cctgcaggaa
     8521 ttaaatgacc tgtaattacg ttttctttta aacctgtcat ataatctact ttaccattaa
     8581 ttgctgctgt acttaatact ttagttgttt cttggaaaga agctgcagaa atccaacttt
     8641 gtacacctaa agaggcttta gtaataccaa gtaatactgg agttgcagtt gcaggtttag
     8701 catctctcac ttcaacagtt tttttatctg ctcttcttaa aatagaattc tcttctctta
     8761 gttcacgaag agtaattatt tttcctgctc tcattttagt gctttcgcca gcatctacca
     8821 ctactttttt atcaaaaatt ctatcatttt ctgcaataaa atctactctg tcttctaaat
     8881 cttcttctaa gaattttgta tcgccagcat ctactatttc tacctttttc atcatctgac
     8941 gaacaattac ttcaatatgt ttatcgttaa tttttacacc ttgcaaacga tatacttctt
     9001 gaatttcgtt tactacatat tcttgtacgg caaaaggacc tttaattgca agaatatctg
     9061 ctggtgcaat ttgtccatca gataatggtg cacctgcttt aataaagtca ccatcttgaa
     9121 caagaatttg acgagataat ggtactagat attttttaat cattccatct ttagattcaa
     9181 ctgcaatttc tctattacca cgttttacat taccaaaagt aactatacca tcaatttcac
     9241 aaacaactgc aggattgcct gggtttcttg cctcaaataa ttctgtaaca cgaggtaaac
     9301 cacccgtaat atctcttaac ttacctaata tacgtggaat tttaactaaa acctgaccag
     9361 ctttaacttc ttctccttcg tcaactgcta agtggctacc tacaggtaag ttatagtgct
     9421 tgttatcttt acctgataca ataactgttg gaattttagt tttatctttt gtttcaataa
     9481 ttactttttc acggtgtcct gtttggtcat cactttcttc tctaaaagta attccatcta
     9541 ttaaactttc aaattctaca gtaccatttt gttctgcaac tattacattg ttaaatggat
     9601 cccatgtaca aataacatca ccctttttaa tttgttgacc atcttttaca agtaatgtag
     9661 agccataagg aacattattt gtaattagta atcggtcgtt tttaacatcc attgttctta
     9721 cttcaccagt acgaccaata actatattta ctttgttacc ttcattattt acactagata
     9781 cagtacgtaa accatcaaac tgaacagtac catcaaactt agcatttaaa gaagattcaa
     9841 cagaagagct gcctgcaaca ccacctacgt ggaaggtacg aagtgtaagc tgtgtaccag
     9901 gttcaccaat agattgggct gcaataattc ctacagcatc gccactttgt gtaatataac
     9961 ctgttgctag gtttttgcca tagcatttaa cacaaacacc acgtttagat tcgcaggtta
    10021 aaacggaacg tatttcaaca gtttcaattc cgctattttc aatttctttg gcaacttccg
    10081 aactaatttc ttcacctgct ttaataatta aggcatctgt tacagggtgt aaaacatcgt
    10141 gtaatgatgt tctaccttct attctatctg ctaatggttc aataatatct tcattatctt
    10201 ttaatgcact aatcgcaatt ccacgtagtg taccacaatc ttcttcagaa attacaacgt
    10261 cttgtgctac atcaactaat ctacgtgtta agtaaccagc atctgcagtt tttaaggctg
    10321 tatccgccaa acctttacga gcaccgtggg tagatataaa gtagtctaat acattcaagc
    10381 cgcctttaaa gttcgaaaga attggatttt caattacctc agaaccgcta gatccgcttt
    10441 tacgtggttt agccattaag cctctaatac caactaactg ttttacctgt gttttacttc
    10501 ctctggcacc actatccaac atcatgaata cagagttaaa tccttgttta tctgtagtca
    10561 ttttatgaat taatgtttca gtaataatca tatcggctct accccaaata tctacaactt
    10621 ggttataacg ttcgttattg gtaattaagc ccatattgta actttcccaa acttcatcaa
    10681 cttctgcctt agcattttct aaaagttcgt ttctttcttc tggtactata aggtcgttta
    10741 cactaaatga aagaccgccc ttaaatgcca ttctaaaacc taaggttttg atatcatcta
    10801 agaacttagc agttttaggt acattggtaa ttttaataat ttctccaata atttctctta
    10861 aacttttttt ggttaaaagt gcatttacat aacctacttc ttttggaaca tgttggttaa
    10921 ataatacacg tcctactgta gtatcaatta gtttgtttac taactgtccg ttttcacgaa
    10981 ctgtagtttt tactttaata ccagcatgta aatcaaccac gttttcattg taagcaatta
    11041 caacttcatc taaactataa aatgtttttc cttcgccttt tacaggttct tctttggtag
    11101 attttttaat ttttgtgata tagtataagc caagtaccat gtcttgagaa ggtaaggtaa
    11161 ttggtgtacc attttgagga tttaaaatgt tgtgagaaga caacattaat aattgagctt
    11221 ctaaaattgc agcactactt aaaggaacgt gtacagccat ttggtcacca tcaaagtcag
    11281 cgttgaaagc tgctgtaact aatgggtgta attgtatagc ttttccttca attaaccttg
    11341 gttgaaaaga ctgaatagat aatctgtgaa gggtaggggc acgatttaat aaaacaggat
    11401 ggccttttag aatattttct aaaatgtccc aaacaattgc ttctttttta tctacgtact
    11461 ttttagcaga ttttacagtt ttaacaatac ctctttctaa taatttacga ataataaatg
    11521 gcttaaataa ttctgcagcc atatctttag gcaatccaca ttcatgcatt tttaattcag
    11581 gtcctactac aattacagaa cgaccagaat agtcaaccct tttacctaat aagttttgac
    11641 ggaaacgacc ttgtttacct tttaaaacat cacttaaaga ttttaacgct ctaccacctt
    11701 cggcttttac tgcatttgat ttgcggctat tatcaaataa gctatcaata gcttcctgta
    11761 acatacgttt ttcgttacgc aaaatcactt cgggagcttt aatttccatc aaacgtttta
    11821 aacgattgtt tctaatgatt acacgacgat ataaatcgtt taaatcagaa gaagcaaaac
    11881 gtccaccatc taaaggtaca agtggtctta attctggtgg aataactgga atatattgca
    11941 taaccatcca ttctggacgg tttgaaattc ttgcagaaga gtctctaaaa gcttccacaa
    12001 cactcaaacg ttttaaagca tctgctttac gttgttgaga tgtttcgttt gctgcagcat
    12061 tacgtaattg atagcttaat tcatcaaggt ctaatctttc taataaatca tgcactgctt
    12121 ctgcacccat tttagcaata aacttttctg ggtcttcatc tggcaaatac tgattttctt
    12181 ttggtaaggt atctataata tccaaatact cttcttcagt taataaatct cctgtaccta
    12241 aactttcttt cacaccacct tgaattacta cataacgttc gtagtaaaca attgtttcta
    12301 attttttaga actcattcct aataaataac caattttatt aggtaaactt ttgaagtacc
    12361 aaatgtgcac aaccggaacc accagtttta tatgccccat tctttctctt cttacttttt
    12421 tttctgtaac ttctacgcca caacgatcac aaacaatgcc tttataacga atacgtttgt
    12481 atttaccaca agcacattca taatctttta cagggccaaa aattctttcg caaaataaac
    12541 catctctttc tggtttatac gtacgatagt tgatagtttc aggttttaaa acttcgccaa
    12601 aacttctttc caaaatagaa tctggagagg ctaggctgat tgtaattttt gaaaaaccag
    12661 gtttggtttt gtttacatct tttttaatag ccatattata gtagatgaaa ttttattttg
    12721 aaatagtcta tcttctcatt cctgttttgg tttccatagc taactgaatg ttggtgaggc
    12781 actcagcatt acttgtacat tcttacacaa ttttgttgta agacaagctg cagtttaaca
    12841 caaaattttt tgaaaaagat acaatatccc aaattgttta aggcggcgaa ggtatgtata
    12901 gtttttgaaa tgaaaaagta attttttgaa ttatttaaaa gctgtaaata catggttttc
    12961 acctaacaaa aagcctttta tgttttgcat aaaaggccta acgtattatt taataaactg
    13021 gtatggttat ttctaactgt ttcttttaat tttaattttt ttctctacaa aaattggaac
    13081 atattctact acagtattac tgtctatttt tacttttact acctcttgat aatttagttt
    13141 tagagcagct aaagtatttg tttttttaat aaagcctaag ggctttttgg ttgtttgtat
    13201 tgttgcagta acatttttgt ttgcaatgct tttatttggc tttgggttaa attcgccagc
    13261 acttgcagta atgcataaaa cagaagttaa gatagtgaaa gttaaatact tgtacatttt
    13321 atttaaatat taaagtttgc taaattgttt tagcttttca ctaaatattg gattactgaa
    13381 ccaatattat tataagaaat actatgccaa accaaaacaa tacaattata aaattaaata
    13441 actcattgat taatagtgaa taattatatt tttttaatta aaaatttaaa tataaattta
    13501 ttaaattttt ccatttttgg cttagttttc caaattgtag aatacataaa ataaaaatcc
    13561 cgataaaaca atgctttatc gggttataaa taaaattaaa agaagctatt tttcaaaata
    13621 agctatatgt ccaccaaccc attcttttcc tttattatat cgtactccta tcattttacc
    13681 tttacttatt ctggctaaat taaaggttgc tattggtcta gcttctccat ctttccaaaa
    13741 ataaaatatt ctcacttcag ctttagctgg cccgtcaggt gttttaataa tatcggcata
    13801 gtttactttt ttttgtaaaa tccaattttc tggatctttc actttactta tatcctcttt
    13861 agtaacatca ataattaccc cttgccctgc aaaagagaat aaaggcttta aaacaaagtt
    13921 ttctaaatct gttggcaatt ctattaagtc tgttaaaaat ttagtttctg gtacaaaagg
    13981 atgattgata taaggtaaag tgtatttact tattctgtaa aaccaattag gatgtggtac
    14041 ccattctacc tctaaatcac ttaataaaat tttgcctttt tcttgtactt cggccgtttg
    14101 ttgttgtaaa tcatcaaaaa ttaacctatt gtatattctt tttacttcaa ttttttttcc
    14161 atctttttcg tagtataatt ttttgccttc taaaattaat tcagttaaac aaatagtttt
    14221 tatacctaaa tatgcttcgg tacaataaaa atcaactctt gttttttgct gatgaggaaa
    14281 tatttctaat agtattacat tttctgctgg atgattgcca acaataattt cttttaaaag
    14341 gtttagatag gattcttcgt ttaaattact taaataaggg gtgtagttat ctggaatgct
    14401 aaaatatttt cttgttactt ggtctaatag tacttgataa gcaaataaag taggaaaacc
    14461 ctgcatttcg attaactgag gttctaattt tccttcgttg ttttcacaaa ctccaaaatc
    14521 aaaagctata aaatgagagt ggttattttc atttgctaca cgaacatttg caggtatgcc
    14581 atgagaggta agtgttttaa aattaaagcc agttataaca tctacaatgt tttcgcagca
    14641 atctatcatt ttttgagtaa attctttatc tacaaaaacg ggagtttcgg ctacacgaaa
    14701 atctaaggca ttggggaatt ttgtattaat gtcttctatt aaagactcgt aattactttg
    14761 gtaaaaatgt tcattaaagt tgtttcttat attaggaacc atagtgaaag taaattaatt
    14821 attttttgaa agataaatta agattgtgaa aaaacagcag gagagttaag aaatctgata
    14881 aaaaattatg cttctgttaa tactcctaca gcatggttta agaaatttgg taaaatgtga
    14941 ttatatgtcc aaagaatttt ttctggatcg cctaattgtt ctaccatgct tctccatttt
    15001 tcttctccat gatttaaaat tacagttgct ttcttttctt cggtaagtaa atgtaatttc
    15061 attccaatag catctgcctc tggatgaaat tgagttccta taaaataatc attaaatctt
    15121 acagccataa tggctctttc aaagggcaca tgaggcctat ttttttcgat tgccaaaatt
    15181 ttagcaccca tttctcttaa gatagaatag ttaggctcaa ttacttgata gtctctgcta
    15241 tctacaacat aaaaaggttc ttttaaattt tcaaaaacca tttcttcatt tccttcttca
    15301 agtttatgaa caggaaaaac gccaaatgag gtagattttc ttttacaaac attgcctatt
    15361 ttatagtgtc tgcaaattaa ttgaaagcta tgacaaataa agaaaacatg ctttggaata
    15421 ttggttgcat tattattcca ttcttctatt gattgtaacc atttaaagta tttttgctcc
    15481 caattagtgc caatagtttc aagaggtgaa cctggaccac cagttgaaat ataaatatga
    15541 taagatgtat ctggaatttc ttctttaagc cttaaatcaa actcatcaaa gtttacttct
    15601 agctgattat gttctgccca agtgtttata atttctctaa tacaacgcat accttgattg
    15661 gcatgccctt catataaatc taatattgca atttttattt tctttgattc tgcccaattc
    15721 attctataac aaacataata ataaattaat acaactaatc caatgcttta tacacattat
    15781 tgaataaagc ttctcaaaat taatagtttt taatttttta ggaaaagaga tttattgttc
    15841 ggtgttggaa ttttctgaaa ttgaattgtt actgttctcc gtatttggaa ggttagtttc
    15901 aacttcatca ataattgtat tgctggtaaa atcatctgtg tttatgatgg taccttctac
    15961 aagttgttca gaaagtattt cttttatttt tggtaaatca tcggtagaat taataccaaa
    16021 ataatccata aatgattttg aagttgtata taaaagtgga tgccctggaa ggttttcgtt
    16081 tctgcctgca attacaataa gttctttttc tagtaatttt tgaacactat aatcactatt
    16141 tacgcctctt aaactttcaa tttcgccttt ggtaattggt tgcttgtatg caatgatggc
    16201 taaagtttct aaagcagcgg tagatagcct ttttagaaat ttatctccat taagttgtgc
    16261 aacagtttga tgaaaatctt ttttagttaa gaactgaaaa ccaccaccac tttctttcac
    16321 ttcaaatgga taaaattcgg tattgtattt ttcaacaatt ccttctatgc agctttcaac
    16381 ctggtctaaa tttattcttt cttctaaaaa accaaaagag ttatttatta gttctacaat
    16441 ttctagtgct gttaaaggtt tttcactagc aaaaattaat acttcaatat gtggtataat
    16501 agcagataac tccatttgca aaagataggt ttattttttt aaattatcct tgtaaagctg
    16561 ctgctccgcc tactatttct gtaagttctg ttgtaattgc tgcttgcctt gcacggttgt
    16621 acgaaatttt taatgttttt aataattcat ttgcattatc acttgcttta tccattgctg
    16681 tcattctagc accatgttcg ctagcattgc catctaaaac tgctttgtat aactgcgtat
    16741 ttaaaatttt aggcatcaat tctgcaatta actcatcttt atttggctca aaaataaagt
    16801 cagatttttt attaatatta gtattgcttt ctactttagg tataggtaaa aaaggttctg
    16861 ctataaaagt ttgtgttgct gcatttttaa aatggctata aataatttct actgcatcaa
    16921 actcgttatt ttcaaaagct ttagttgctg cttgtgcagc agcttgtaca ttagtaaaag
    16981 atagttgttg aaagatatct ttaaaagtgt cgctggtttt atagcctgct ttggttaaag
    17041 cctcccaacc cttcttacca atattccaaa tagctacatt tcctttttga tattgggtag
    17101 aatacttttg atcaatggta ctttttgcta gttttataat attactgttg taggcaccac
    17161 ataaacctct atcactagtt acaacaataa gcaatacttt ttctactggt ctattttcgg
    17221 caagttttaa ttctgtatcg ccatcaatat tacttacaat attgctaagc atttcttgta
    17281 atttttgggc ataaggacgc atttgtgtta tagcatcttg tgccctgcgt aactttgctg
    17341 cactaaccat tttcatagct ttggtaattt gctgtgtgct ttgtacgctt tttattctat
    17401 ttcttacttc ttttaattga cctgccattt tgaattaagt ttataaaact ttaatagtta
    17461 aaattggctg caaaagtagc agaataaggc taaaatacca tattcagttt ttaatgtgaa
    17521 aaattattgg tttttaggcg gtttattaac tgtttttagt taagttatac caacgcactt
    17581 gcacctaata tagcagcatc ggcctcgtgt aaattgctta aaactaattt tactttattt
    17641 ttaaaaatgg gtaagaggtt cttttccatt gctttttgtg taggaataag taaaagatct
    17701 cctgctttgg ctatgcctcc aaaaagaaca accatttctg gagaggaaaa cattacaaaa
    17761 tttgcaaggg cttctcctaa aatttcgcct gtaaattcaa aaacctgttt ggctatttta
    17821 tcttcctgta ttgcacaatt ataaattatt tcgctggtaa tttcttcagg ttgatagttt
    17881 cgtagaaggc ttgagttatt ggttgaagat aatagttcta aagcagtttg ttttattcca
    17941 tttgcactgg cataagcctc taaactgcca taggccccag tgcctttatg gtatcttcca
    18001 ttatggcgaa taatggtatg gcctaattct cctgcaaatc catcatggcc taaaacaatg
    18061 tttccatcaa ttacaattcc actacctaca cctgttccta aagtgattgt aataaaatgt
    18121 ttaatttctt tagctttgcc atatttcatt tctccaaaag ctgctgcatt agcatcgttg
    18181 gttaagaagg ttggtaaatt aaatgctttt tgtattaagc ttgctaatgg tataatttct
    18241 ttccatatta aattaggggc atactcaatt gtaccagtat aaaagttgcc attaggagca
    18301 ccaatgccaa ttcctttaat agcatttttt ccaccatatt tttcaattaa tggatttatg
    18361 acttgatata attctgctat aaataaatta atatcagcat gctgtgtaga agatatctta
    18421 ttttgctcta gaatttcacc tttagaattt acgattccaa attttgttga agttcctcct
    18481 atatcaattc caatagcaag tttatttgta tttgtaagca ttgtaatttg aagaaattta
    18541 tttaaggttg aagctagcca cctgctgttt tttcataccc ttcatctatt aataaattgt
    18601 tggcatcggc agcagtagtt gctaattcat cgcaacggtt gttaaaaaca ttttctgcat
    18661 gtccttttac ccattgcatt ttaattttaa agtctttgga aagagtataa aattctagcc
    18721 aaaggtcttt gtttttcttt cctcctttaa aatcagtttt aatccagtta tctaaccatt
    18781 ttttttctat actatctacc acatatttac tatctgtata aatggtaaca gatatatttt
    18841 ttttggttaa ctgttttaat gctactataa cagccattaa ctccattctg ttattggtag
    18901 ttaaacgaaa gcctgcagca atttcttttt tggtattgcc ccatagcatt acaataccaa
    18961 agccaccttt gcctggattt cctctgcttg caccatcggt atatattttt aataaatgca
    19021 aaagataatt tgaaaatttt acacttgaaa gacaccggtt ttaaattctg gaataatatt
    19081 ttgatggttg gctgcatgaa tggcttcaga aattaattgc cttgtttgta atgggtcaat
    19141 aatttcatca acccacagcc ttgctgctgc ataataaggg gttgtttggt tattatattt
    19201 gcttttcagt ttatctagta attctgtttc ttcttcttgt gttaattctt taccttttgc
    19261 tttcatggtg gctacctgta tttgtaacat tgttttggct gcttgctctc cgcccataac
    19321 agcaattttt gcactgggcc atgcataaat aaaccgagga tcgtatgctt tgccacacat
    19381 tgcataattg ccagcaccat aactgttacc cacaattatg gttattttgg gtactacaga
    19441 attggcaaca gcattaacaa gtttagcacc atcttttatt ataccactat gttcactacg
    19501 gctgcctacc ataaaacctg taacatcttg taaaaaaatt aatggtattt tcttttggtt
    19561 gcaattcata ataaaacgtg cagctttatc agcactgtcg ttataaatta caccacccat
    19621 ttgcatttcg ccttttttgc tttttacaat taaacgttgg ttagctacaa tgcctactgc
    19681 ccaaccttct attctggcat agccacataa aatagttttg ccataatctt ttttaaattc
    19741 atcaaattcc gaggcatcta ccattcgttc aattatctct ctcatgtcat aaggttttgc
    19801 atttccttcg ggcatgatgc tataaatttc ttggctattt tttgttggtt caattgcatt
    19861 aattctatca aatcctgcag tgggttggtg gcttatttta ctaataattt tttttatttg
    19921 ttgtaagcac tcttgttctg ttttaaactt ataatctgca attccgctaa tttcagtatg
    19981 ggtaattgca ccaccaagtg tttcattatc tatgtcttcg ccaattgcag cttttaccaa
    20041 ataactacca gctaaaaaaa tactaccatt attttcaacc atcaaagttt catcgctcat
    20101 tattggtaaa tatgcaccac ctgcaacaca ggcacccata actgcagcaa tttgaatgat
    20161 gccattagca ctcatttttg cattgtttct aaaaattcta ccaaaatgtt ctttgtccgg
    20221 aaagatttca tcttgcatag gcaagtacac acctgcacta tctactaaat aaattatagg
    20281 taaattattt tcgtctgcaa tttcttgcat tcttaaattc tttttacctg ttataggaaa
    20341 ccaagcacct gctttaactg tttggtcgtt tgcaacaata atacattgcc tattacatat
    20401 atatccaata ccagcaacag taccacctgc agggcagcca ccttcctctg catacatttc
    20461 aaaacctgca aaagagccta attcaataaa gggtttatct ttatcgcaga ggtattctat
    20521 tctttctctt gcagttaatt tatttttttc gtgttgctta tctatggctt ttttaccacc
    20581 acctaactta attttagata ggtgttgttt tacctcgcta agctttaact tcatccaatc
    20641 ttcgtttcta tttagttcta cattcatcgt acttacagtt gttagtttgc aaagataggg
    20701 atgtggttaa aagtaaaatt tgtttacatg tttaaaataa tcgcttaaaa gaatctattc
    20761 aattttaaaa aaataaaagg ctttctcggt aagagaaaac ctttagaata taagagacaa
    20821 acaatgaaaa attttaattg caatattttt caaattcagt tattaatttt tgtgcaattg
    20881 cttgtgcagg atatccttct atttgatgac gctcaaacat gcttactaag ttgccatctt
    20941 taaacaaggc aatagcagga gaggatggag ggtaaggcat taaatattct cttacctttt
    21001 gtactgcttc tgtatcgtac ccagcaaaac tggttgctaa attatcgggt ttggttgttg
    21061 cattatttac agccataatt actccaggtc tgcaagcacc tgcagcacag ccacaaacac
    21121 tatttataac tactaaagtt gt
//
LOCUS       DAOQPK010000011        20906 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_1234_length_20906_cov_4.803511, whole genome shotgun sequence.
ACCESSION   DAOQPK010000011 DAOQPK010000000
VERSION     DAOQPK010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 20906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20906
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1234_length_20906_cov_4.803511"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PLU36_01100"
     CDS             <1..262
                     /locus_tag="PLU36_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45376.1"
                     /translation="IPKEIKRWYHRKDSIRVAKEKEKEKEMEQREDITPDSTTNIKNT
                     FDPEAEPNRKENDKNNNPSTLDQPPLLLHNTEKKKKKDSIKP"
     gene            259..1602
                     /gene="rodA"
                     /locus_tag="PLU36_01105"
     CDS             259..1602
                     /gene="rodA"
                     /locus_tag="PLU36_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HMZ45377.1"
                     /translation="MSIQHNSMRVSQKTDTLIIWLYAILVTIGICCIFMVEYKPGMNV
                     MQNILGGTTNFSKQTLFAGVCVIVATFIMLSDSKLFTAFANLFYAFGIVLMLATFVLG
                     KNINGSKSWIPLGGGFNLQPAELCKIFVSLALAKYLSRQETDFSKLKSQIIGAAIAMV
                     PAILSIAQQETGLALVYSAFFIVMYREGLPSTILITGISVIILVVATLLLEPNTLAII
                     LTLIAAIIIFILRRQIKRQKNILTIIVTLWLVCVSIQRFAVPYFFDNVLKCYQSTRVY
                     SMVGKDYDCTKNKHASNNTSKAVRKPDDYNVRQSKIAIGSGGVIGKGYLKGTQTRGKY
                     VPEQSTDFIFTSIGEAFGFVGSFIFLLLYLLLLLRIIRLAERQRSTFSRVYAYSVVSI
                     LFFHITVNICMTIGLFPIIGIPLPLISYGGSSLLTFTILIFIMLRLDADRQMVLR"
     gene            complement(1664..2077)
                     /locus_tag="PLU36_01110"
     CDS             complement(1664..2077)
                     /locus_tag="PLU36_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45378.1"
                     /translation="MKKILAIAITLLGFAMHSNAQGKITDVPANTTNEKINSKQAKFE
                     KEMLAALTEAGLTEEEIKKVRAIQDDAYKKTGEIRKDNSLSKEQKEEKAKEINKDKNR
                     KIREVIGKEKFSKFMDIRKRQREEKSTNKEDKSAE"
     gene            complement(2106..3134)
                     /locus_tag="PLU36_01115"
     CDS             complement(2106..3134)
                     /locus_tag="PLU36_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HMZ45379.1"
                     /translation="MKKILPLLTVLFAVCTISTNAQQRPYYTQYILNNFIVNPAVAGI
                     ENYTDVKLSHRMQWVGLQDAPVTTYFTIHAPLKKDVYERETATSIHARGENPRGKNFV
                     ADYTAPSNHAGIGLSIINDKIGPLNNFIATATYAYHMGVGPHTSLSAGVSAGISNLRL
                     NAAKLQFANGTQDPAVAGSGYLNNVRPDVSAGLWLYDRDFFIGLAAQQIVPQKVTYSN
                     NHVGLEQGKLVPHLFLSAGYRLFFTDDLTFLPSAVLRYVSPMPIGFDLNAKIQYQDYI
                     WAGASYRYKDGYAAMVGINLSNSINIGYSYDLTTSRLNTVSKGTHEFVLGFILGNKYG
                     DWCPRYNF"
     gene            3342..3415
                     /locus_tag="PLU36_01120"
     tRNA            3342..3415
                     /locus_tag="PLU36_01120"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3374..3376,aa:Gln,seq:ttg)
     gene            3560..4519
                     /locus_tag="PLU36_01125"
     CDS             3560..4519
                     /locus_tag="PLU36_01125"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HMZ45380.1"
                     /translation="MMNPSVKVFSGTGSQKLAEKIAQRFGTPLGKVNIQKFSDGEFQP
                     IFLESIRGDYVFLIQSTFAPTENLMELLLMIDAAKRASASRIVAVIPYYGFARQDRKD
                     KPRVAIGSKLIATLLQAAGADRIITMDLHAPQIQGFFDIPVDHLDSSAIFIPYIEQLQ
                     LGNLTFAAPDVGSTNRVREIASYFNAEMVICDKHRKRANEIASMVVIGDVTNKDIVLI
                     DDICDTGGTLAKSAALLKEKGAKSVRAFCTHPVLSGKAYENIENSVLEELVVCDTIPL
                     KQESGKIKTISVADLFAIAIRNAYENKSITSLFVHSQLRGRDK"
     gene            4537..4917
                     /locus_tag="PLU36_01130"
     CDS             4537..4917
                     /locus_tag="PLU36_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45381.1"
                     /translation="MRLKYLVFLIILSSFIGYLEWGTESKTFLIIAEAEIIHKIITHP
                     SSVIHPFILIPLLGQLLLVYQLIKNKLQKIILYLAISCIALLFIFMLFIAITTLNLKI
                     LLSVMPFFVVSIYTLYSIKKSPIN"
     gene            complement(4955..5272)
                     /gene="trxA"
                     /locus_tag="PLU36_01135"
     CDS             complement(4955..5272)
                     /gene="trxA"
                     /locus_tag="PLU36_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HMZ45382.1"
                     /translation="MALEFTDANFQQEVIESDKLSVVDFWAEWCGPCRAIGPVIEELA
                     KEYEGKVKIGKVNVDVNPNVSVNYGITSIPAILFIKGGKIVDKQIGAVPKNVLDKKIQ
                     ANM"
     gene            complement(5425..5772)
                     /locus_tag="PLU36_01140"
     CDS             complement(5425..5772)
                     /locus_tag="PLU36_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45383.1"
                     /translation="MTEKEPILFQNDCNEQEMFDYLNNNLTPQQRHAFELKMEENVFL
                     QEAVEGLEKIQNKQKVHQFITELNHNLDKQLERKRIKKHKRKIKTQGWIIISIVIILA
                     LCVLGYLVIKQIK"
     gene            complement(5769..6338)
                     /locus_tag="PLU36_01145"
     CDS             complement(5769..6338)
                     /locus_tag="PLU36_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ45384.1"
                     /translation="MQPANYQHITDKELLNLYNSSNENKWIGILLQRYTLLLFGICFK
                     YLKNEEAAKDAVQQIFLHAIAEIKKYEITYFKSWIYVLARNYCLMQLRKKNVYIEITD
                     SHVDENDTKYNIEKLLEKEETLNLLEKALQQLNQEQKTCLSLFYLQKKTYQQVSEATG
                     YSLLQVKSFIQNGKRNLKLLIQKNSTQKI"
     gene            6555..7739
                     /locus_tag="PLU36_01150"
     CDS             6555..7739
                     /locus_tag="PLU36_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMZ45385.1"
                     /translation="MAARADLFQSPDYFALDELLSEEHKLIRETVRNYVKKEISPIIE
                     DYAQRAEFPEQIVKQMGELGCFGPTVSVEYGGGGLDYISYGLMMQELERGDSGVRSTA
                     SVQGSLVMYPIYAYGSEEQKRKYLPKLANGEFLGCFGLTEPNHGSDPSSMLTTIKDAG
                     DHVILNGAKMWISNAPYAHVAVVWAKDEHNIVRGLIVERGMPGFSTPTTHGKWSLRAS
                     ATGELVFDNVKVPKENIFPNVQGLKGPLGCLTKARFGIAWGALGAAMDCYDTALRYSK
                     ERIQFDKPIAGFQLQQKKLAEMVTEITKAQLMVWRLGVLMNEGKATPQQVSMAKRNSC
                     EIATNIARDARQMLGGMGITGEYSIMRHMMNLESVITYEGTHDIHLLITGMDVTGINA
                     FK"
     gene            7794..8930
                     /locus_tag="PLU36_01155"
     CDS             7794..8930
                     /locus_tag="PLU36_01155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ45386.1"
                     /translation="MNKHLHIISFTVPYPPNYGGVVDLFWKLPALQKAGIQIHLHCFE
                     YGQNLQTELNKYCASVHYYKRLVGLNGLSFTLPYIVASRKNEELFLKLLEDDYPILLE
                     GIHSTYLLNDNRFENRKIIVRLHNVECNYYKHLANNSNNLLNKLYYLAEHLLLKKYEK
                     KLATTKAIFLTVTEKDKSTFIHQFGYKNINYLPLFLPESWKFNLATTKNSYCLYQADL
                     SVVNNEKAAIWLLEKVFNTIDFPLIVAGKNPSLKLIQIVKKYKHVTLNANPNNEVMQQ
                     LISHAQINILPSLSNTGIKLKLLNALYNGKYCLVNSNTIDGTEFESLCIVANNVEEMQ
                     QKIKELFCMSFNEPEIIKRKTVLDKIYNNTQNAEQLIKYIFDES"
     gene            8954..9394
                     /gene="smpB"
                     /locus_tag="PLU36_01160"
     CDS             8954..9394
                     /gene="smpB"
                     /locus_tag="PLU36_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HMZ45387.1"
                     /translation="MTKEMNNRQAYFNYFIEDKYVAGIVLLGTEVKSIREGKVSFNDA
                     FCLLVDGQLWLRGFYIAEYSHGTVNNHIAVHDRKLLLQKREIKKLQNKLKDKGLTIVP
                     LKVYLNEKNLVKVEIGLAKGKKLHDKRDTIKQRDAERELKRFVK"
     gene            9414..9974
                     /locus_tag="PLU36_01165"
     CDS             9414..9974
                     /locus_tag="PLU36_01165"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HMZ45388.1"
                     /translation="MKANDKSFLVYVAKLVLLFLALDYGTTFFIGITTPGGSHYVPWL
                     DKYFNYVNWLRQTILQSSKFCMGILGYNMELVSPYILKIKNGAGVHLVYSCLGYGVIS
                     FWIAFVLANKATLWLKVKWLLAGVAIIIFTNILRVCILIIALQKKMFKPFTLDHHTFY
                     NIVAYSVVIILMLLFIKQIRKQQLKV"
     gene            complement(9982..11175)
                     /locus_tag="PLU36_01170"
     CDS             complement(9982..11175)
                     /locus_tag="PLU36_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HMZ45389.1"
                     /translation="MNNRQIFQQHLAQTSPAPIAIEMNKAEGIYLFDVDGKKYIDAIS
                     GFSVANIGHTNSTVVEAVTKQAKEYMHLIVYGEFIQQPQTAYAKLLTDNLPSDLNCVY
                     FTNSGTEATEGALKLAKRATGRTKIYAFNNAYHGSTQGSLSVIGSEYWRNNFRPLLPE
                     VYHFNYNSNEIIEAIDSTVACVIIEIVQAEAGIKAANKTWLQTLYKKCKANGVLFIVD
                     EIQSGFGRTGSLWAFEQFEIIPDILLLGKALGGGMPLGAFIANYSLMNLLTNNPVLGH
                     ITTFGGHPISCVAGKAALEVLLTENLLNNIQHKQSILINNLTKKLIKHVNYIGLWAAI
                     AFENFETNHAVIQKCITKGLITDWFLFAPNCMRLAPPLISTNQQLLDICSIINESIDE
                     VELNQ"
     gene            complement(11207..11899)
                     /locus_tag="PLU36_01175"
     CDS             complement(11207..11899)
                     /locus_tag="PLU36_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45390.1"
                     /translation="MKKLGFLFLILLCFQSIVFSQDATTLLNKVKAKINLVNDYTATG
                     KMKTDITFIKAPIGKVTVYFKKPNKFKLKKENGISILPKGGVSINIGAIINVEDFDII
                     DSGEEIIDNTITRKIILLPKNETNDVALSTMYIDAEKLLVKKVVTTTKENGTYQILLS
                     YGNYSQYGLPDKVVFGFNTKDYKMPKGITMEFDDEGKKEMREKMKNKKGKVEIMYNTY
                     IINKGINDAIFN"
     gene            complement(11911..12786)
                     /locus_tag="PLU36_01180"
     CDS             complement(11911..12786)
                     /locus_tag="PLU36_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HMZ45391.1"
                     /translation="MFTLIQIELYKIFNRPRTYIAFIAIAALTIIVQLGLKVDGKEYA
                     DFVMADLKNTFSIDGKIINGYFVCYVLLQLLLVHVPLLIALIAADMISGEANIGTLRL
                     LLTKPISRVQFIIAKFIATCIYTVALLIWLAILGLLLSIFIFGTGDLLLLKNNYVVQL
                     QQNDIFWRYTGAFLFAAIAMITVAAVGFFLSLFAENSIGPIVATMSIIIVCTILSTMS
                     LPLFNLIKPYLFTTHLVTWKEFFDIKINNQNETIAGSIQNPAKIINSLTVLIAHIIIL
                     ISITIFIFKKKDILS"
     gene            complement(12788..13699)
                     /locus_tag="PLU36_01185"
     CDS             complement(12788..13699)
                     /locus_tag="PLU36_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ45392.1"
                     /translation="MNEIIKVTNLTKQFKQITAVNNLSFNVNSGDVYGFLGQNGAGKS
                     TTIRMLLTLIQPTNGSIEIFGLPLQNNRNEILKNIGAVIEKPDVYKYLSAYDNLKLFA
                     RLNGTRPTHHQLMQQLEIVGLANRAKDKVATFSQGMKQRVGIAIALIHQPQLIILDEP
                     TNGLDPQGIADIRNLIIKLSKEHGKTILISSHLLSEIEQIATRILIIDKGKKIIEDTA
                     TALFNPSQTIVEIETTNNEFALQQLQKSKWLTSIQQHTTNTITFKLDKSQIPTLNSNL
                     VEMQIQLISIKPRHSLEDYFLRVTTAN"
     gene            13855..14457
                     /locus_tag="PLU36_01190"
     CDS             13855..14457
                     /locus_tag="PLU36_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HMZ45393.1"
                     /translation="MVKNIFIIALMGMVNIVIAQQSSQSNKTISVCLSNSEMKLYNLV
                     MEYRLQNGLPSIPLSASLCFVAQQHCKDLINNKPKTSVCNMHSWSSNGSWSSCCYTPD
                     HKNAACMWNKPRELTNYKGNGYEIAFYVWSSASATYTAEPEEALKGWKGSPGHNDVII
                     NKNIWKSANWNSIGIGIYYGYATIWFGEEKDTETIPQVCN"
     gene            14505..15272
                     /gene="rsmA"
                     /locus_tag="PLU36_01195"
     CDS             14505..15272
                     /gene="rsmA"
                     /locus_tag="PLU36_01195"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HMZ45394.1"
                     /translation="MQYTLKKALGQHFLKDENICKNITDVINQSLNETTISNLLEVGP
                     GAGAITKYLLQIPGINFKVVELDAEKVAYLNATYTAIQSKIIHQNFLEMHAPFDEKFM
                     VVGNFPYNISSQILFKILEWKQNVPVVVGMFQKEVAQRVAAQPGSKTYGIISILIQAY
                     YDVEYLFDVSANCFNPPPKVMSGVIQLKLKDKTFSKRSDKDFINLVKTAFNQRRKMLR
                     NAVKNLFSEDELKNEIFNKRAEQLSIEDFAALTFKMK"
     gene            15277..16203
                     /locus_tag="PLU36_01200"
     CDS             15277..16203
                     /locus_tag="PLU36_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ45395.1"
                     /translation="MKRIIISGYAHSVLQEMLSNAGFEVIYNPTISYQELLNEIEPAV
                     GLITTTRIKADEMLLRKATNLKFIGRLGSGMDLIDVEFAKQKNIKCLNSPEGNRNAVA
                     EHTLALLLNLMNKINISYDALKQNIWAREENRGDELEGKIVGIIGFGNTGKRFAELLQ
                     PFNVTVLAYDKYKFGFASNYIKQASFEQICKQANVISFHVPLTAETHYYANDSFFNSL
                     QQQPYFLNTCRGKVTNTKALINALENKKIKAAGLDVLENEQLSTYTTDEKIMLQQLIR
                     FNNVIITPHTAGVTAQSLFKMSKIMANKILDL"
     gene            complement(16216..16668)
                     /gene="dtd"
                     /locus_tag="PLU36_01205"
     CDS             complement(16216..16668)
                     /gene="dtd"
                     /locus_tag="PLU36_01205"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HMZ45396.1"
                     /translation="MKVVIQRVTNASVTVNNVVVAKINTGLLVLLGIEDSDTNEDIIW
                     LSNKIVNLRIFNDEDGVMNKSVLELHGDILLVSQFTLHASTKKGNRPSYIKASKPNTA
                     IPIYEKMKIQLEQDCNKKIETGIFGADMKVSLLNDGPVTIIIDTKNKE"
     gene            16703..17110
                     /locus_tag="PLU36_01210"
     CDS             16703..17110
                     /locus_tag="PLU36_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HMZ45397.1"
                     /translation="MKIDISKEIIFKTARSGGKGGQNVNKVETMVEARWDINATTLVT
                     EEEKELLKEVLKNKISINGFLILKSQQSRTQLGNKEIVVNKMNNLINVAIQKKKSRIA
                     TKTPNAVIEQRKENKIIKSKQKQNRKRINLDEL"
     gene            17107..17820
                     /locus_tag="PLU36_01215"
     CDS             17107..17820
                     /locus_tag="PLU36_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45398.1"
                     /translation="MIIKNLTTTLFCTLLITIAVAQETTTIQLKHKVGDELLQLGTTS
                     YTNQWNEQFTVNKFKYYITALEVEFTNGNKNYSWHKKYHLVDEANAASKTIVLDKKLN
                     NIKAIHFLLGVDSIVNTNGIHTGDLDPMKTMFWTWNTGYIYAKLEGQSSSSKAQAHSF
                     SYHIGGYKANENAVKKISLLIKDNATTDNEIIIEANILAWFNGAKKLKIAEEPICHQP
                     GKLAMDIANNYEKMFSVKL"
     gene            17826..18884
                     /locus_tag="PLU36_01220"
     CDS             17826..18884
                     /locus_tag="PLU36_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HMZ45399.1"
                     /translation="MKTFKVITILICLVVVGVGFIEIKKHKPTPLIFIVPKGWPKPIY
                     NFKNNPLTVEGFELGKKLFYDERLSKDSTISCASCHQQFSAFATFDHNFSHGYNNTFT
                     TRNAPALQNLAWQKELMWDGGINHLDVQPITPIVAENEMAETLENVIYKLKKDTSYKR
                     MFKNAFGSNEITTERMTKALSQFMVMLVSSNSKYDKVKRGEDTFNLPEKLGYEIFKQK
                     CASCHTEPFFTDLSYRNTGMPLDATINDIGRMRITNNKNDSLKFKVPSLRNVDLTFPY
                     GHDGRFFTLSNVFEHYRSNMQPIHNTDILLKNKLYLSNFQIGQLTAFLFTLTDSSFTK
                     NPLFAAPAYTKPIILHQH"
     gene            complement(19105..19947)
                     /gene="tsf"
                     /locus_tag="PLU36_01225"
     CDS             complement(19105..19947)
                     /gene="tsf"
                     /locus_tag="PLU36_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HMZ45400.1"
                     /translation="MSTTTAITAADINKLRQATGAGMMDCRKALTETGGDFEAAIDWL
                     RKQGQKIAAKRSDREAKEGVVIAQTTADNKTGFVVCISCETDFVSKNADFVAFAQSIA
                     DAAVANNVNSIEELNNVSINGAKVSDLINDKLAAIGEKIGIVKFEKVEAPYVASYIHG
                     AYRLGVLVALSAAEAEAGKDVAMQIAALNPVAVDANSVSADVIERERNIIIETMKQDP
                     KMAGKPDDMIAKIAEGKLNAFFKEQTLLAQAFVKDGSKTVAEYLTSVQKDLQVISFKR
                     VALG"
     gene            complement(20120..>20906)
                     /gene="rpsB"
                     /locus_tag="PLU36_01230"
     CDS             complement(20120..>20906)
                     /gene="rpsB"
                     /locus_tag="PLU36_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HMZ45401.1"
                     /translation="KGIHIIDLNKTVVHLQETAAALKQIAKSGKKIMFVATKKQAKEI
                     VSECAKRVNMPYATERWLGGMLTNFNTVRKSVKKMQSIDKMLNDGSAESLTKKERLTL
                     SRDKEKMEKVLGGIAQLGRLPSALFIVDIGVEHIALAEAKRLGISTFGVVDTNCDPNK
                     VDFAIPANDDATKSIAIITNYITAAIAEGLAERMANKEEEEANQNDDNNENEAKAKRL
                     EQEAQAEGGRGRGRGGRTTGDGATNANNAPKRRVPNTRRPAGK"
BASE COUNT         7210 a         3051 c         3201 g         7444 t
ORIGIN      
        1 aataccgaaa gaaattaaac gttggtacca tagaaaagat tctattagag tagcaaaaga
       61 aaaagaaaaa gaaaaagaga tggagcaaag agaagatata acaccagata gtactacaaa
      121 tattaaaaac acttttgatc cggaagctga gccaaacaga aaagaaaatg ataaaaacaa
      181 taatccatcc acattagatc agcctccttt attattacac aatacagaaa aaaagaaaaa
      241 gaaagatagt ataaagccat gagtattcag cacaatagca tgagggtttc gcaaaaaaca
      301 gataccttaa taatatggct ttatgccata ttggttacaa ttggtatatg ttgcattttt
      361 atggttgaat ataaacctgg aatgaatgtg atgcaaaaca ttttaggagg tacaaccaat
      421 tttagtaaac aaacactatt tgctggggta tgtgtaattg tggcaacttt tattatgcta
      481 agcgatagta aactttttac agcatttgca aatttatttt atgcttttgg cattgtatta
      541 atgctggcaa cttttgtgtt aggcaaaaac attaatggca gtaaaagttg gataccatta
      601 ggtggaggct ttaatttaca accagccgaa ctatgcaaaa tatttgtatc attggcattg
      661 gctaaatatt tatcaaggca agaaacagac ttctctaaat taaaatctca aattattgga
      721 gcagctattg caatggtgcc tgccattctt tctattgccc agcaagaaac aggtttggca
      781 cttgtttact ctgctttttt tattgtaatg tatagagaag ggctcccttc taccatactt
      841 attacaggta tttctgtaat tattcttgtg gtagcaactt tattattaga gccaaatact
      901 ttggcaataa tattaacttt aattgctgct attataattt ttatacttag aaggcaaata
      961 aaaaggcaaa aaaatattct aaccattata gttacacttt ggcttgtttg tgtaagtatt
     1021 caacgttttg ctgtgcccta tttttttgat aatgttttaa aatgttatca aagtacaaga
     1081 gtgtatagca tggtaggtaa agattatgat tgcactaaaa ataaacatgc ttctaacaac
     1141 actagtaaag ctgtaagaaa accagatgat tataatgtaa ggcaaagtaa aattgcaatt
     1201 ggctctggag gggtaatagg caaaggctat ttaaaaggta ctcaaacacg aggtaaatat
     1261 gtacctgaac aaagtacaga ttttattttt acttccattg gtgaggcatt tggatttgtt
     1321 ggtagtttta tttttttact tctgtactta ttacttttat tacgaattat taggttagca
     1381 gaaagacaaa ggagtacatt tagcagggtt tatgcttata gcgtagtaag cattttgttt
     1441 tttcatatta ctgtaaatat ttgtatgact attggtttat ttcctataat tggtattcca
     1501 ttaccattaa ttagttatgg gggctcatcg ttacttactt ttactatctt aatatttatt
     1561 atgctaaggt tagatgcaga taggcaaatg gttttaagat agaaataaaa aagctgacaa
     1621 taagtatgtt tatgtcagct ttttttataa aatttttact taattattca gcactcttgt
     1681 cttctttatt agttgatttc tcttctcttt gtcttttcct tatatccata aatttagaaa
     1741 atttttcctt gccaattact tctctaattt ttctattttt atctttgttt atttcttttg
     1801 ctttctcctc tttctgttct ttacttaaac tattgtcttt tctaatttct ccggtttttt
     1861 tgtatgcgtc atcttgtatt gctctaactt ttttaatttc ttcttcagtt aaaccagctt
     1921 ctgttaatgc agcaagcatt tctttttcaa actttgcttg tttgctattt attttttcat
     1981 ttgtagtatt tgcaggtaca tccgtaattt ttccttgtgc attgctatgc attgcaaaac
     2041 ccaataaggt aattgcaatt gctaatattt ttttcattgt tgaaatattt taaataaatg
     2101 tagaattaaa aattatatct agggcaccaa tcgccatatt tatttcctaa aataaaccct
     2161 aaaacaaatt catgagtgcc tttacttaca gtatttaaac gagatgttgt taaatcgtaa
     2221 gaatagccaa tattaatact attgcttaaa ttaataccaa ccatagcagc ataaccatct
     2281 ttatatctgt aggaagcacc agcccaaata taatcttggt attgaatttt tgcattcaaa
     2341 tcaaaaccta taggcattgg gcttacatat cttaatactg cagatggtaa aaaagttaaa
     2401 tcatcagtaa aaaataaacg atagccagca cttaaaaata aatgaggtac aagtttacct
     2461 tgttctaagc ctacatggtt attgctataa gttacttttt gaggtacaat ttgctgtgca
     2521 gctaagccaa taaaaaaatc tctatcgtaa agccataggc ctgcacttac atctggtcta
     2581 acattattta aatatccact accagcaact gcagggtctt gtgtgccatt ggcaaattgt
     2641 aatttagcag catttaatct taggttagaa ataccagcac taacacctgc acttaaggaa
     2701 gtgtgtggac caacacccat gtgatatgca taggttgctg tagcaataaa attatttaaa
     2761 gggcctattt tatcgttaat aatagataag ccaatacctg catggttgga tggtgctgta
     2821 taatctgcta caaagttttt ccctcttggg ttttcgcccc tagcatgaat actggtagct
     2881 gtttctcttt cataaacatc tttctttaaa ggggcatgaa ttgtaaaata agtagttaca
     2941 ggtgcatctt gcaaaccaac ccattgcata cgatggctta attttacatc agtgtaattt
     3001 tcaataccag caactgcagg gtttacaata aaattgttta aaatgtactg tgtgtaataa
     3061 ggtctttgct gagcatttgt tgaaatagtg caaactgcaa ataatacagt tagtaggggt
     3121 aaaatctttt tcatacgttc caaaaataaa ggaaagatgc aaacatatta tgttatgctg
     3181 tttattgatg cttaaatttt aaatattttt ttattttgta tataatattt ttctgaaaat
     3241 tctgaaaata aaatagttat aaaatatttt ttgaaatctt tgtttatttt tttggataaa
     3301 gtttttgtag cctttacctt tgccgtccat ttttaaggtt ttggcctata gtataatggt
     3361 agtacgacag attttggttc tgttcgtctt ggttcgaatc caggtaggcc aaccaaataa
     3421 cccacttttt gtgggttttt ttttatattt cctacaaaat aaagtctttt caatactaat
     3481 tcacaattct taaacatact ataatttcaa ttttttagct tataacatac gtttatcttt
     3541 gcagcacatt atacccatta tgatgaatcc ttctgttaaa gtattttcag gcacaggttc
     3601 tcaaaaacta gccgaaaaaa ttgctcaacg ttttggtaca ccacttggaa aagtaaatat
     3661 tcaaaaattt agtgatggtg aatttcaacc cattttttta gaaagtatta gaggcgatta
     3721 tgttttttta attcaaagca catttgcacc caccgaaaat ttaatggaac ttttgctaat
     3781 gatagatgct gctaaaagag caagtgccag tagaattgtg gctgttattc catattatgg
     3841 ttttgcaagg caagatagaa aggataaacc acgtgttgca attggcagta aattaattgc
     3901 tacacttcta caagctgcag gtgcagatag aattattaca atggatttac acgcaccaca
     3961 aattcaaggc ttttttgata ttcctgtaga tcatttagat agtagtgcca tttttatacc
     4021 ttatattgaa caattacaat taggaaactt aacttttgct gcaccagatg taggtagtac
     4081 taaccgagta agagaaattg ccagttattt taatgcagaa atggttattt gcgataaaca
     4141 tcgtaaacgt gccaacgaaa ttgctagcat ggttgttata ggagatgtta ccaataaaga
     4201 cattgtgttg atagatgata tttgtgatac aggaggaaca cttgctaaaa gtgcagctct
     4261 attaaaagaa aaaggtgcca aaagtgttag agccttttgt acacatcctg ttttaagtgg
     4321 caaagcatac gaaaatattg aaaatagtgt tttagaagaa ctagtagtat gcgatactat
     4381 tcctttaaaa caagaaagtg gtaagattaa aactatttca gttgccgatt tgtttgcaat
     4441 agctatacgc aatgcttatg aaaataaaag tataaccagt ttatttgttc atagccagtt
     4501 aagaggcaga gataagtaaa aaatttatct ctttttatga ggcttaaata tcttgtattt
     4561 ttaattattc tatcttcttt tattggatat ttagaatggg gtaccgaaag taaaaccttt
     4621 ttaattattg cagaagccga aataattcat aaaattatta ctcacccttc ctctgttatt
     4681 catcctttta tattaatacc attattaggg caattacttt tagtttatca gttaattaaa
     4741 aataaactac aaaaaattat tttatattta gcaataagtt gtattgcctt gctttttata
     4801 tttatgcttt ttattgcaat tacaacactt aatttaaaaa ttcttttatc tgtaatgcct
     4861 ttttttgttg taagtattta tactttatat agtataaaaa aaagccccat taattaaact
     4921 aattactagg gcttaaaatt ttaaacaaat attattacat gtttgcctga atttttttat
     4981 ctaatacatt tttaggtaca gcaccaattt gtttatctac tattttacca cctttaataa
     5041 acaatatggc tggtatagat gtaatgccat agtttacact aacattgggg ttaacatcca
     5101 catttacttt tcctatcttt acttttcctt catattcttt tgctaactct tctattacag
     5161 ggccaattgc tctacatggt ccacaccatt ctgcccaaaa atcaacaacc gataatttat
     5221 cactttctat tacttcttgc tgaaagtttg catctgtaaa ttctaatgcc ataattgtaa
     5281 atttaattta ttttaataaa acttttcctt gagtttaaac atcaaatata tatccaattt
     5341 acattttgtg ctgttttgac ttttccacca atgttattta tgactattta agctcaaaac
     5401 tgccattggg gcatattatt gtaattactt aatttgcttt ataaccaaat agcctaaaac
     5461 acaaagtgct aaaattatta caatagaaat aattatccag ccttgtgttt ttatttttct
     5521 tttatgtttt tttatccttt tgcgttctaa ttgtttatcc aggttatggt ttagttcagt
     5581 aataaattga tgtacttttt gtttgttttg tatcttttct aaaccttcca ctgcttcttg
     5641 caaaaaaaca ttctcttcca ttttaagctc aaatgcatgg cgttgttgag gtgttaaatt
     5701 attattcaaa taatcaaaca tttcttgttc gttacagtcg ttttgaaaca atattggctc
     5761 tttttctgtc atattttttg agtcgagttt ttttgaatta gaagctttaa atttcttttg
     5821 ccattttgta taaaactttt aacctgtaat aaagaataac ctgttgcttc tgatacttgc
     5881 tgataggttt tcttttgtaa ataaaataac gataaacatg ttttttgttc ctggtttaat
     5941 tgttgtaaag ccttttctaa taagtttaat gtttcttctt tctccaatag tttttcaata
     6001 ttatattttg tatcattttc atcaacatga gaatcggtta tttcaatata cacatttttc
     6061 tttcttaatt gcattaaaca atagtttctg gccaacacgt aaatccaact tttaaaataa
     6121 gttatttcat atttttttat ttcggcaatt gcatgcaaaa atatttgttg tacagcatct
     6181 ttggctgcct cttcgttctt taaatattta aagcaaatgc caaaaagtaa taatgtatat
     6241 cgttgtaata aaattccaat ccatttattt tcattactgg agttgtaaag atttaaaagt
     6301 tctttatcag taatatgttg gtagttagca ggctgcacta tacaccttta ttgcatagta
     6361 aacttaataa agtttaatta atttttctaa ttaaagagta atttttttga gaagtttttt
     6421 gctgtttaat gccaatcttt aaaaccaaat aaaatagaat ttattcactt acttttgcat
     6481 aaatttttaa agaaatttta ataccaactt taactttggt ttaattcatt tcatcaacaa
     6541 aaaaaatatt ttacatggca gcaagagcag atttatttca aagcccagat tattttgcat
     6601 tagacgaatt attatcagaa gagcacaagt taattagaga aactgttcgt aattatgtta
     6661 aaaaagaaat atctccaatt atagaagatt atgctcaacg tgccgaattt ccagaacaaa
     6721 ttgttaaaca aatgggtgag ttaggatgtt ttggtccaac tgtttctgtt gaatatggtg
     6781 gtggaggttt agattatatt tcttacggat taatgatgca agaattagaa agaggagata
     6841 gtggagtaag aagtactgca agtgtacaag gcagtttagt tatgtatcct atatatgcct
     6901 atggtagcga agaacaaaaa agaaaatatt tacctaaact tgcaaatggt gaatttcttg
     6961 gatgttttgg gctaacagaa cctaatcatg gtagtgaccc tagtagtatg ctaactacca
     7021 taaaagatgc aggagatcat gtaattttaa atggagcaaa aatgtggatt agcaatgcac
     7081 cttatgctca tgttgcagtt gtatgggcaa aagatgaaca taatatagta agaggcctaa
     7141 ttgtggaacg tggaatgcct gggttttcta ccccaacaac acatggtaaa tggagtttaa
     7201 gagctagtgc aacaggtgaa ttagtttttg ataatgtaaa agtacctaaa gaaaatattt
     7261 ttcctaatgt acaaggctta aaagggccat tgggctgttt aactaaagca agatttggaa
     7321 ttgcttgggg tgctttaggg gctgcaatgg attgctacga tactgcttta agatatagta
     7381 aagaaagaat acaatttgat aaaccaattg ctggttttca gttacaacaa aaaaaattag
     7441 ctgaaatggt taccgaaatt accaaagcac agctaatggt ttggcgttta ggagtattaa
     7501 tgaacgaagg aaaagcaacg ccacaacaag taagtatggc taaaagaaat agctgcgaaa
     7561 ttgctacgaa tattgccaga gatgctaggc aaatgcttgg tggtatgggc attacaggag
     7621 aatatagtat tatgagacac atgatgaatt tagaaagtgt aattacttac gaaggcactc
     7681 atgatattca cttgttaatt actggtatgg atgtaaccgg tattaatgca tttaaataat
     7741 aaactatttc taaattattt taagaatatt gcactgtatt caacagtaag tatttgaaca
     7801 aacatttgca tataatttct tttacagttc cttatccacc taattatggc ggggtagttg
     7861 atttgttttg gaaattacca gcactacaaa aagcaggtat tcaaatacat ttacattgtt
     7921 ttgaatatgg acaaaactta caaacagaat taaataaata ttgtgcatct gttcactatt
     7981 ataaaagatt agttggctta aatgggctaa gctttacttt accttacatt gttgcctcta
     8041 gaaagaacga agaattattt ttgaaattat tagaagatga ttatcctatt ttgttagagg
     8101 gtattcatag tacctattta ttgaatgata acagatttga gaatagaaaa attattgtac
     8161 gattacacaa tgtagagtgt aattattata aacatttagc taataatagc aacaacttat
     8221 taaataaact ttattatcta gccgagcatt tattattaaa aaaatacgaa aaaaaattag
     8281 ctaccacaaa ggctattttt ttaactgtaa cagaaaaaga taagtcaact tttatccatc
     8341 aattcgggta taaaaatatt aattatttgc ctttgttttt accagaaagt tggaaattta
     8401 atttagcaac tacaaaaaat tcttattgtt tataccaagc agacttaagt gttgttaata
     8461 acgaaaaagc tgcaatttgg ttattggaga aagtatttaa tactattgat tttcctttaa
     8521 ttgttgcagg caaaaatcca tcattaaaac taattcaaat tgtaaaaaaa tataaacacg
     8581 ttactttaaa tgctaaccca aacaacgaag taatgcagca attaattagc catgcacaaa
     8641 ttaatatttt accctcttta agtaatacag gtattaaatt aaaattatta aatgcacttt
     8701 ataatggcaa atattgcttg gtaaatagta atacaataga tgggactgaa tttgaaagtt
     8761 tatgtatagt tgccaataat gtggaagaga tgcagcaaaa aataaaagag ttattttgta
     8821 tgagttttaa tgaaccggaa ataataaaac gaaaaaccgt attagataaa atatacaata
     8881 acacacaaaa tgcagaacaa ttaataaaat atatctttga tgaaagttga ggcaaccaag
     8941 cagtattaat accatgacaa aagaaatgaa taataggcag gcttatttta attacttcat
     9001 agaagataag tatgttgcag gtattgtatt attaggcact gaagtaaaaa gtattagaga
     9061 aggaaaagta agttttaatg atgcattttg tttgttggtt gatgggcaat tatggttaag
     9121 aggtttttat attgcagaat attcacatgg tactgtaaac aaccatattg ctgtacacga
     9181 tagaaaattg cttttacaaa aaagagaaat taaaaagcta caaaataaat taaaagataa
     9241 aggtttaacc atagtgcctc ttaaagttta tttgaacgaa aaaaatttgg taaaagtaga
     9301 aattggttta gcgaaaggca aaaaattaca cgataaaaga gataccatta aacaaagaga
     9361 tgctgaacgt gaattaaagc gttttgtaaa ataacaactc aaaatttatt atagtgaagg
     9421 ctaatgacaa atcattttta gtttatgttg caaagctggt gctgttattt ttagcattag
     9481 attatggcac tacttttttt attggtatta caacacctgg aggaagccat tatgtgcctt
     9541 ggttggataa gtattttaat tatgttaatt ggcttaggca aactatatta caatcttcta
     9601 agttttgtat gggcatattg ggatacaata tggaacttgt tagcccttat attttaaaaa
     9661 taaaaaatgg agcaggtgta catttagttt attcatgctt gggctatggt gttattagtt
     9721 tttggatagc atttgtatta gctaacaaag ctactttatg gctaaaagta aaatggttat
     9781 tggctggggt cgcaataatt atttttacta atattttaag agtatgtatt ttaattattg
     9841 cattacaaaa gaaaatgttt aagccattta ccttagatca tcacacattt tataatattg
     9901 ttgcttactc tgtagtaatt attttgatgt tactttttat caagcaaata agaaagcaac
     9961 aactaaaagt ataaattaga tttattgatt aagttcaacc tcatcaatac tttcattaat
    10021 aatactacaa atatcaagta attgttgatt ggtactaatt aatggaggag ccaaacgcat
    10081 acagtttgga gcaaataaaa accaatctgt aattaagcct tttgttatgc atttttgaat
    10141 aacggcatga ttagtttcaa aattttcaaa agcaatagca gcccacaaac caatataatt
    10201 tacatgctta attaattttt ttgtaagatt atttataaga attgattgtt tgtgctgaat
    10261 gttatttaat aaattttctg ttaataaaac ctctaaagca gccttacctg caacacaact
    10321 tataggatgc ccaccaaagg tggtaatatg gcctaataca gggttattgg ttaataaatt
    10381 cattaaagag tagttggcaa taaaagcacc taaaggcata ccaccaccca atgcttttcc
    10441 taaaagtaaa atatcaggta ttatttcaaa ttgttcaaaa gcccataaac ttcctgttct
    10501 tccaaaacca ctttgtattt catcaacaat aaaaagtaca ccattggctt tgcatttttt
    10561 atacaatgtt tgaagccaag ttttgttggc agctttaata ccagcttcag cttgcactat
    10621 ttctataatt acacaagcta cagtagagtc tattgcttca ataatttcat tagagttata
    10681 attaaagtgg taaacttcgg gcaacaaagg acgaaagtta tttctccaat attcacttcc
    10741 aataacactt aaagagcctt gtgtgcttcc atgataagca ttgttgaatg cataaatttt
    10801 ggttctgccg gttgctcttt ttgctaattt taaagcacct tctgttgctt ctgttccact
    10861 atttgtaaag taaacacagt ttaaatctga aggtaaatta tcagtgagta atttagcata
    10921 ggcagtttgt ggttgctgaa taaactcacc atatacaata aggtgcatgt attcttttgc
    10981 ttgcttggtt acagcttcta caactgtaga atttgtgtgt cctatattgg ctacactaaa
    11041 tccagaaatt gcatcaatat attttttccc atcaacatca aacaaataaa ttccttcggc
    11101 tttattcatt tctattgcaa ttggtgcagg agatgtttgt gcaagatgtt gctgaaagat
    11161 ttgcctgtta ttcattacca gttattttat aataatatag caaaagttaa ttaaaaatgg
    11221 catcattaat gcctttattt ataatgtatg tgttatacat aatttcaact tttccttttt
    11281 tatttttcat tttttctctc atttcttttt tcccttcatc atcaaactcc attgttatgc
    11341 ctttaggcat tttgtaatct ttagtattaa aaccaaaaac aactttatcg ggtaacccat
    11401 attggctata atttccataa cttaataaaa tttgataagt gccgttttct tttgttgttg
    11461 taactacttt ttttactaat aatttttctg catcaatata catggtactt aacgcaacat
    11521 cgtttgtttc atttttgggt aataatatta tttttcttgt tatagtattg tcaataattt
    11581 cttcaccact atctataata tcaaaatcct ctacattaat aattgctcca atatttatag
    11641 atacaccacc ttttggtaaa atagatattc cattttcttt tttaagctta aacttatttg
    11701 gctttttgaa ataaactgta acctttccga ttggggcttt aataaaagtt atatctgttt
    11761 tcattttgcc agttgcagta taatcattta ctaaatttat ttttgctttt actttattta
    11821 gtaatgtggt tgcatcttgc gaaaaaacaa ttgattgaaa acacaagagt attaaaaata
    11881 gaaatcctaa cttcttcatt gttgaaaatt ttagcttaat atatctttct ttttaaaaat
    11941 aaaaatggta atacttatta gaataataat atgagcaatt aaaacggtta agctattaat
    12001 aatttttgct gggttttgaa tacttcctgc aatagtttcg ttttggttat taatttttat
    12061 atcaaaaaat tctttccaag taaccaaatg ggttgtaaac aagtaaggtt ttattaaatt
    12121 aaataaaggt aaactcatag tacttaaaat ggtacaaact ataataatac tcattgttgc
    12181 aacaattggg ccaatagagt tttcggcaaa taaagaaaga aaaaaaccaa cagcagcaac
    12241 tgttatcatt gcaattgctg caaataaaaa tgcaccagtg tatcgccaaa aaatatcgtt
    12301 ttgctgaagt tgtacaacat aattattttt tagtaacaat aaatctcctg tgccaaaaat
    12361 aaatatactt agtaataaac ctagaattgc aagccatata agtaaagcaa cagtatatat
    12421 acaggtggca ataaattttg caataataaa ttgcactcta ctaattggtt ttgttagcaa
    12481 taatcttaag gttccaatat tggcttcacc gctaatcata tctgctgcaa ttaaagcaat
    12541 tagcaaaggc acatgcacta atagcaattg taatagaaca taacatacaa aatatccgtt
    12601 tattattttt ccgtcaatac taaaagtgtt ttttaaatcg gccattacaa agtctgcata
    12661 ttctttacca tctactttta atcctaactg cacaataatg gttaatgcag ctattgcaat
    12721 aaaagcaata tatgttctgg gacggttaaa tattttatat aattctattt gtattaaagt
    12781 aaacatatta attggcagtg gttactcgta aaaaataatc ttctaaacta tgtcttggtt
    12841 taatgctaat aagctgaatt tgcatttcaa ccaaattgct attcaaggtt ggtatttggc
    12901 ttttatctaa tttaaaagta atggtattag tagtatgttg ctgaatgctt gttagccatt
    12961 tacttttttg tagttgctgc aaagcaaatt cattattggt tgtttctatt tcaacaattg
    13021 tttgcgatgg gttaaacaaa gcggtagcag tatcttcaat aattttttta cctttatcaa
    13081 ttatcaaaat tcttgttgca atttgttcta tttcacttaa aaggtgcgat gagattaaaa
    13141 tagttttacc atgctcttta cttaatttta ttattaaatt tcttatatct gctatgcctt
    13201 gaggatctaa accattagtt ggttcatcta aaataattaa ctgtggctgg tgtattaatg
    13261 caatagcaat tcctactctt tgtttcatgc cttgagagaa ggttgcaact ttatcttttg
    13321 ctctatttgc tagcccaaca atttctaatt gttgcattag ttgatgatgt gttggcctgg
    13381 taccatttag cctggcaaaa agttttaaat tatcgtaagc acttaagtat ttatatacat
    13441 ctggtttttc tattactgca ccaatatttt ttaatatttc atttctatta ttttgtagtg
    13501 gtaagccaaa aatttctata ctaccattag tgggttgtat taatgtaagt aacattctaa
    13561 tggttgtact ttttccggct ccattttgcc ctaaaaagcc atacacatca ccactattaa
    13621 cattaaaaga taaattattt actgctgtaa tttgcttaaa ctgcttagtt aaatttgtaa
    13681 ccttaattat ttcattcatg taatatagcc aattggggca aaggtagtag ctacatatat
    13741 tgcatagtag gttttgtata ataaatttga taaattgatg tgaaaaatac tatttgcttt
    13801 ttttaagtaa aaagaaacct tatttttaag tatcaaattt ttattcaact tataatggta
    13861 aaaaatattt tcattattgc tttaatgggt atggtaaata ttgttatcgc acaacaatca
    13921 tcacaaagca ataaaaccat aagtgtttgt ttaagtaata gtgaaatgaa attgtataat
    13981 ttagtgatgg aatacaggct gcaaaatgga ttaccctcta tacctctttc tgcatcttta
    14041 tgttttgtag cacaacagca ttgtaaagat ttgattaata acaaacctaa aacttctgtg
    14101 tgcaatatgc atagttggag tagtaatgga agttggagtt cttgttgtta tacgccagat
    14161 cataaaaatg ctgcttgcat gtggaataag ccaagagagc taaccaatta taaaggtaat
    14221 ggatacgaga ttgcttttta tgtttggagt tcggcttcag ccacatatac tgcagaacct
    14281 gaagaagctt taaaaggatg gaagggaagc cctggacata acgatgttat aattaataaa
    14341 aatatttgga aatcggctaa ttggaattca attggtattg gtatctatta tggttatgct
    14401 accatttggt ttggagaaga aaaggataca gaaacaatac cacaggtttg taattaattt
    14461 tttccatttt aattgcaata ttatttgatg ttacatttgc agcaatgcaa tacacactaa
    14521 aaaaagcatt aggccagcat tttttaaaag atgaaaacat atgtaaaaat attacagatg
    14581 taattaatca gtctttgaat gaaactacta ttagtaattt attagaagtt ggacctggtg
    14641 ctggtgctat tacaaaatac ttgcttcaaa tacctggtat aaattttaaa gttgttgaat
    14701 tagatgcaga aaaagtagct tacttaaatg ctacttatac tgctatacaa tctaaaataa
    14761 ttcatcaaaa ttttttagaa atgcatgcac catttgatga aaaatttatg gtggtaggta
    14821 attttcctta taatatttct tctcaaatat tatttaaaat attagaatgg aaacagaatg
    14881 tacctgttgt tgttggcatg tttcaaaaag aagtagccca acgtgttgca gcacaacctg
    14941 gcagcaaaac atacggtatc attagtatat taattcaggc ttattatgat gtagaatatt
    15001 tatttgatgt ttctgcaaat tgtttcaatc ctccaccaaa ggttatgagt ggtgtaattc
    15061 aacttaaatt aaaagataaa accttcagca aaagaagcga taaagatttt attaatttag
    15121 taaaaaccgc ttttaatcaa cgaagaaaaa tgcttagaaa tgcagttaaa aatttatttt
    15181 cagaagatga gttaaagaat gaaattttta ataaacgagc agagcagtta agtattgaag
    15241 actttgctgc cctaactttt aaaatgaaat aaaaaattga aaagaataat tatttctggt
    15301 tatgctcatt ctgtattgca agaaatgtta agcaatgcag gctttgaggt aatatataac
    15361 ccaactatta gctaccaaga attgttgaat gaaattgaac ctgcagttgg gttaattaca
    15421 acaactagaa ttaaggcaga tgaaatgctt ttgagaaaag caactaattt aaaatttata
    15481 ggtaggttag gtagcggaat ggatttaatt gatgtggagt ttgcaaaaca aaaaaatata
    15541 aaatgtttaa atagtccaga gggaaataga aatgcagtag ctgaacatac attggcattg
    15601 ttgttaaacc ttatgaataa aataaacata agttacgatg cattaaagca aaatatttgg
    15661 gcaagagaag aaaatagggg agatgagtta gagggtaaaa tagttggtat tattggcttt
    15721 ggtaatacag gtaaaagatt tgctgaactt ctacaacctt ttaatgttac tgtattagct
    15781 tatgataaat ataagtttgg gtttgcatct aactatatca agcaagccag ctttgagcaa
    15841 atttgcaaac aagctaatgt aattagtttt catgtgccac ttacagcaga aacgcattat
    15901 tatgcaaatg attctttttt taactcttta caacaacagc cttatttttt aaatacttgc
    15961 agaggtaaag taaccaatac aaaagcttta attaatgcct tagaaaataa aaaaattaaa
    16021 gcagcaggct tagatgtttt agaaaatgaa caattatcta cttatactac agatgaaaaa
    16081 attatgctgc agcaactaat ccgttttaat aatgtaatta ttacaccaca tacagcaggt
    16141 gttacagcac aatctttgtt taaaatgagt aaaataatgg caaataaaat tttagatctt
    16201 taactgcttc ctacattatt ctttattttt ggtatctata ataatcgtta ctggtccatc
    16261 attcagcaag cttactttca tatctgcacc aaaaattcct gtttcaattt ttttattaca
    16321 gtcttgctct aattgtatct tcattttttc ataaatggga attgcagtgt ttggtttact
    16381 tgctttaata taagatggcc tattaccttt tttggtagat gcatgtaagg taaactggct
    16441 tactaataaa atatctccat gcaactccaa aacacttttg ttcattactc catcctcatc
    16501 attaaaaatt ctaagattaa ctattttgtt gcttagccaa ataatatctt catttgtatc
    16561 agagtcttct attcctaata aaactaataa accagtatta atttttgcaa caactacatt
    16621 atttactgta acacttgcat tagttactct ttgtatgacc actttcataa aataaaatat
    16681 aaaatgattg tacttttatg aaatgaagat agatataagc aaggaaatta tttttaaaac
    16741 agctagaagt ggtggcaaag gaggacaaaa tgtaaataaa gttgaaacca tggtagaggc
    16801 aagatgggat attaatgcta caacattagt aacagaagaa gaaaaggaat tattgaagga
    16861 agttttaaaa aacaaaattt ctatcaatgg ctttttaatt ctaaaatctc aacaaagcag
    16921 aacacaatta ggtaataaag aaattgtggt taataaaatg aataatttaa ttaatgttgc
    16981 catacaaaaa aagaaaagta gaattgctac caaaacccct aatgctgtta tagaacagag
    17041 gaaggaaaat aaaataataa agtctaaaca aaaacaaaac agaaaaagaa taaaccttga
    17101 tgaattatga taattaaaaa tttaaccact actctttttt gtactttatt aattacaatt
    17161 gccgtagcac aagaaacaac aaccatacaa ttaaagcata aagtaggaga tgagttgttg
    17221 caacttggta ctacatctta cacaaatcaa tggaatgaac agtttacagt aaataagttt
    17281 aaatactaca ttactgcctt agaagttgaa tttacaaatg gtaacaaaaa ttatagttgg
    17341 cataagaaat atcatttggt agatgaggcc aatgctgcat caaaaacaat tgtattagat
    17401 aaaaagctca acaatattaa agcaattcat tttttgttag gggtagatag tattgtaaat
    17461 acgaatggca ttcatactgg cgatttagac cctatgaaaa caatgttttg gacatggaat
    17521 actggctata tatatgcaaa actagaaggg caaagtagtt cctctaaagc acaagcacat
    17581 agctttagtt atcatattgg tggttacaaa gcaaatgaaa atgcagttaa aaaaatatca
    17641 cttttaatta aggataatgc tacaactgat aacgaaataa ttatagaagc taatattttg
    17701 gcttggttta atggggccaa aaaattaaaa attgccgaag aaccaatttg tcatcaacca
    17761 ggtaaattag caatggatat tgcaaataat tatgaaaaaa tgttttcagt aaaattgtaa
    17821 ctcaaatgaa aacttttaaa gtaataacca tacttatttg tttagtagtt gtaggagttg
    17881 gttttattga aattaaaaaa cataaaccta cacctttaat atttattgtt cccaaaggtt
    17941 ggccaaaacc aatttataat tttaagaaca acccattaac tgtagaaggt tttgaattag
    18001 gtaaaaagct tttttacgat gaaagattaa gcaaagacag cacaattagt tgtgcaagtt
    18061 gccaccagca atttagtgct tttgccactt ttgatcataa ttttagccat gggtataaca
    18121 acacttttac aacacgtaat gcaccagcat tacaaaattt agcttggcaa aaagagttga
    18181 tgtgggatgg tggaataaat catttagatg tacagcctat aacacccatt gtagcagaaa
    18241 atgaaatggc agaaacctta gaaaatgtaa tttataaatt aaaaaaagat accagctaca
    18301 aacgaatgtt taaaaatgct tttggtagca atgaaattac aacagaaaga atgaccaaag
    18361 ctttaagcca atttatggtt atgctagtta gtagtaatag taaatatgat aaagtaaaaa
    18421 gaggagaaga tacttttaac cttcctgaaa aattaggcta cgaaatattt aaacaaaaat
    18481 gtgctagctg ccataccgaa ccatttttta cagatttaag ttatagaaac actggaatgc
    18541 ctttagatgc tacaattaac gatataggaa gaatgagaat aaccaataat aaaaacgatt
    18601 ctttaaaatt taaagttccc tctttacgca atgtagattt aacttttccg tatgggcatg
    18661 atggtaggtt ttttacatta agcaatgtgt ttgagcatta cagaagtaat atgcagccta
    18721 tacataatac agatatactt ctaaaaaata aattgtattt atccaacttt caaattgggc
    18781 agttaactgc atttttattt acattaaccg atagcagttt tacaaaaaat cctctgtttg
    18841 cagcccctgc ttatactaag ccaataattt tacatcaaca ctaaagacta agtaattata
    18901 ggttataata ttttaagaat tttttatgag gtaacttttt acaccataat taactacaaa
    18961 atgaaaattc tttttattat taaacctgca ttactctctt tgctaatttg tttattttgt
    19021 atataaaaat gcccccaatt agtgtctaac tttttggggg cagtgcaaaa aagtggcttt
    19081 ttattttata gaatttgaaa aaaattaacc taaagctact ctcttaaagc taattacttg
    19141 taaatctttc tgtacagatg ttaagtattc agcaactgtt ttgctgccat cttttacaaa
    19201 agcctgagct aataaagttt gttctttaaa gaaagcattt agtttgcctt ctgcaatttt
    19261 agcaatcata tcatcaggtt tacctgccat tttaggatct tgcttcatgg tttcgataat
    19321 aatatttctt tctctttcaa taacatcagc agatacgctg ttagcatcta cagctacagg
    19381 gtttaaagct gcaatttgca tagcaacatc tttacctgct tctgcttctg cagcacttaa
    19441 agctaccaac actcctaaac gataagcacc atgtatataa gatgcaacat aaggagcttc
    19501 tactttttca aattttacaa ttcctatttt ttcgccaata gctgcaagtt tgtcattaat
    19561 taaatcgcta actttagctc cattaatgct aacattattt aactcttcaa ttgaatttac
    19621 attgttggct actgctgcat ctgcaatgct ttgagcaaaa gccacaaagt cggcattttt
    19681 agaaacgaag tctgtttcgc agctaataca aacaacaaaa cctgttttat tatcagctgt
    19741 tgtttgtgca attactacac cttcttttgc ttctctatcg ctacgttttg cagcaatttt
    19801 ttgtccttgc ttacgcaacc agtcaatagc agcttcaaaa tcgccgcctg tttctgttaa
    19861 tgcttttctg caatccatca tgccagcacc tgttgcctga cgtaatttgt taatatctgc
    19921 agcagttata gctgttgttg tactcatggt attttaagtt taatattttt tataaggtta
    19981 tgtttaagat atttattatt gaaaatatgt ttagtataat aaataatatt ttgaatgaaa
    20041 aattaataaa tagataaaaa gcaaattggc tgactataca aataatcaac caattaattg
    20101 ctattattta actgataaat tatttacctg ctggtctgcg agtattaggt actctacgtt
    20161 taggagcatt attagcatta gtagcaccat caccagttgt tctaccacct ctgccacgtc
    20221 cacgacctcc ttcagcttgt gcttcttgtt ctagtctttt tgccttagct tcattttcgt
    20281 tattgtcgtc gttttggttt gcttcttctt cttctttatt tgccatacgt tcagccaagc
    20341 cttctgcaat tgctgcagta atataattgg taataatggc aatagatttt gtagcatcat
    20401 catttgcagg aattgcaaaa tctactttat tagggtcgca attagtatcc acaactccaa
    20461 aagtagaaat acctaaacgc ttagcttctg ctaatgcaat atgctctaca ccaatatcaa
    20521 caataaataa agctgatggt aaacgaccta attgagcaat accacccaat actttttcca
    20581 ttttttcttt atcacggcta agagttaaac gttctttttt agttaggctt tctgcactac
    20641 catcgtttaa cattttatca atgctctgca tttttttaac acttttgcgt acagtattaa
    20701 aattggtaag cataccacct aaccaacgtt ctgttgcata aggcatattt acacgtttag
    20761 cacattcgct aactatttct tttgcttgtt tttttgttgc aacaaacata atttttttac
    20821 cgctttttgc aatttgcttt aaagcagcag ctgtttcttg taagtgaaca acagttttat
    20881 ttaaatcaat aatatgaata cctttt
//
LOCUS       DAOQPK010000012        20390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_1287_length_20390_cov_4.459602, whole genome shotgun sequence.
ACCESSION   DAOQPK010000012 DAOQPK010000000
VERSION     DAOQPK010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 20390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20390
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1287_length_20390_cov_4.459602"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1961)
                     /locus_tag="PLU36_01235"
     CDS             complement(<1..1961)
                     /locus_tag="PLU36_01235"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HMZ45402.1"
                     /translation="MKNKHFNWLVLFFLTITTITIIAACNKKFDEPDTTFVDPNLPIT
                     MTLKELKALLPTTGTVVEITVDKTISGIVVADDKSGNFYKSISIQDATGGVSLRLDGT
                     NLYTSYPVGRRVYINVKGLCVGEYGGMIQLGAVNKATSPISQTPIPQNLFDKYVIKGG
                     FGNVITPKIFSSITDLKTTLQDTVQNTLIQLENFQFSAADTLKTFADPSLASSAVNLN
                     IQNCSGNTITLRTSSYSNFAGIQVPKKKGTITAIYTFFNGTKQLLIRDTSDVQFKDDR
                     CGSSISPTLVYKSIQDVRAMGDGAIMPANTGIRGTIVSNTLNESNGNYRIQDGSGYGL
                     QLRFPSINPNFALNDSVAVDISGLSLSLFNGDLQITNVVNAIKLATGTVVPRNTTVSQ
                     INSNINSWSSTVVKLTNVSIAETSSNATGKNYTVTDASGSIVSFVRTTLGVSSLPANA
                     TTFIGYVSLYNGTPQITIRNAADVVAGGSGPGGGGSTTGITLGSTSPFTLNFNGIGSG
                     LPTGVSVYTGATTTSFGTAGSFNSALGLWANFSAGFKNFASATGLSATSDQTAQDAAS
                     NRALGVRQTSSTGYDPGASFVFLLDNTTGKNNVQLQFLLQSLDNSVGRTVTWTVDYAL
                     GDSPASFTAASASGTLTTSNVFSSNTINV"
     gene            complement(2013..4610)
                     /locus_tag="PLU36_01240"
     CDS             complement(2013..4610)
                     /locus_tag="PLU36_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ45403.1"
                     /translation="MLKFLKFAIILLMAVPISVMAQKQKNKSAKTNGSAVVDTTITDD
                     VKDAMSDNIAVVTIDENDQGDGGAQNVSSVLTAGRDPFFSVMSFNFNAFRFKMRGYDG
                     DLNTTYLNGTPMDNLDNGFTPFGLWGGLNDVMRNRDVSLGLRPSTFAFGDIASSSSID
                     ARASKQRKQTQIGYAISNRNYTHRLTVYHGTGISKKGWAFVFAGSFRGADEAYVPGTY
                     YNGYSYFIGVDKRFGQKHLLSLSLFEAPTESGRQSGVVDELRTITGDNFYNPTWGLQN
                     GKKRNANIAKSSQPTFILNDEYRFNNRTTISTSISATWGKRSTSALDWYNAPDPRPDY
                     YRYLPSYQLSSELQQQVLEAMQTNINKRQINWHGLYEVNRNSFAAIQDANGIQGNTVA
                     GKRSRYIQEERVIDTRRLNFNSVFNTSVGKNIEMTGGISFQSQTNNYYKEVADLLGGE
                     FYVNVNQYAERSFPNNNTANQNDLNTPNRILYKGDKFGYDYDITIRKSSAWLQSVFKF
                     KRVDLFVAGELSSTQFWRTGYTQTGIFPNNSFGKSDVNSFNNYAVKGGVTYKLDGRNY
                     IYANGAILTRAPLFDNVYISPRTRDFIQDNTTNESIQTAEAGYVLNAPKIKVRLTGYF
                     TKIDNQMNVMTFYHDDYRNFVNYAMSNIDKLHFGLEFGAEAKIMRNVTLNVAVAAGRY
                     YYNSRQDATVTVDNDATVLTKDLIYSQNYRLAGTPQNTFSLGFSYRSPKFWFVSLSAN
                     YAANRWLDFNPIRRTFDAVEGLDITKPNEAAIRESILAQTKLEDDYTIDFFGGWSWKL
                     PKSFGFKKSTYLVFNLGINNLLNNKNIISGGFEQLRFDYADKNINKFPPKLYYAYGLN
                     YFGSVTLRF"
     gene            complement(4720..5979)
                     /locus_tag="PLU36_01245"
     CDS             complement(4720..5979)
                     /locus_tag="PLU36_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HMZ45404.1"
                     /translation="MNKLSKLAESLVGSEIVKLGNAISERIRNGETIYNYTIGDFNPS
                     VFPIPTLLEELIIEEYRNKSTNYPPADGVVELRNAISYYLHEWLHLNYNEKEIQVASG
                     GRPIIYTIFKAIVDEGDKVIYGVPSWNNNHYVHLTNGQHCLIDCTPENNFMPLPEDVA
                     KHIKGATLICLCTPQNPTGTTISEESLGKICDLIIEENNSRGEHEKKLYLMFDQMYWM
                     LTMDNIQHFNPVTLRPAMKQYTIFVGGISKSLCATGVRVGWGFGPEHVISKMKAILSH
                     IGAWAPMAEQKATAKYLVNTPALKEFVSNFNKEIDYRLQHIYAGFIALKNKGYQIDAI
                     TPQAAIYLTVKIDLVGKQTNEGNILYTQEDVTAYLLNEAKLAIVPFYCFGASKNSAWY
                     RLSIGTSHKQDIPIMLQKLENALSKLK"
     gene            6179..7702
                     /gene="lysS"
                     /locus_tag="PLU36_01250"
     CDS             6179..7702
                     /gene="lysS"
                     /locus_tag="PLU36_01250"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HMZ45405.1"
                     /translation="MSRQLSEQEIIRREKLQKITNAGIDAFPAPLYPVNTYSADIKNN
                     FTEENKDTFSKVCVAGRIMSINDKGKVFFIKIQDSKGIIQLYVKRDEICPEEDKSFWD
                     NIIKHGLDLGDIIGCTGYVFITKTGETSIHAQTITLLTKSLKPLPVVKRDEEGNVFDE
                     VTDPEFRYRQRYADLIINPSVKETFTKRTKLVNTIREYLNNLGALEVDTPVLQAIPGG
                     AAARPFITHHNALDVPFYLRIANELYLKRLIVGGFDWVYEFSRNFRNEGMDRTHNPEF
                     TILEWYTAYKDYFWMMEITEQLLEKIAVALNGTTTLQVGDKTISFKAPFRRISIFDAI
                     KENTGIDISEKDEIGLREVCKQLNIDVPPTIGKGKLIDEIFGATSEHTYIQPTFIIDY
                     PVEMSPLTKKHRSKKGLVERFELMINGKEIANAYSELNDPIDQRERFEEQAKLMERGD
                     DEAMFIDYDFLRALEYGMPPTSGIGIGIDRLVMLMTNQQSIQDVLFFPQMRPEKQEE"
     gene            7758..8795
                     /locus_tag="PLU36_01255"
     CDS             7758..8795
                     /locus_tag="PLU36_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HMZ45406.1"
                     /translation="MSDLSKFNPNSLGNPNNNIFGLPFTEEEASLILLPVPWEVTVSY
                     GAGTARSAEHIFNAALQVDLFDVDAPNVWKKGFYMLPVDKKLLMKSDYLRKEAELYID
                     FIAKEENFEGNKFMCKNLKEINEGCNYMNNFVYQQTKQLLGNNKFVGLIGGDHSTTFG
                     YIKSLSEKYNQFGILQIDAHCDLRVAYENFTYSHASIMYNILSEFEQVNKLVQLGIRD
                     YSDDEYNYIKNSNGRVTTFFDRDIKHRMYEGEIWKNIVSEIVDALPNNIYISFDVDGL
                     DPKLCPNTGTPVAGGFETEQIVYLFNKLKESNKKIIGFDLVETGVGENDWDSNVSARL
                     LWKMCNLVMNQ"
     gene            8806..9333
                     /locus_tag="PLU36_01260"
     CDS             8806..9333
                     /locus_tag="PLU36_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45407.1"
                     /translation="MQFNFSVFNNRKKQIWLLAYGLMIVNGLIFLLFALKSNIYKLAF
                     IYSALLITTTLVSIFIKKYKSIFYNVSYLLIAFSWIQLNYPWIGVLLIILLSVTKKFT
                     ADIAVTINEKEVEIKNVFSNNYQWSSFQNVILKDKLLTLDFKNNKILQVEILNKTTLE
                     QEKEFNEFCQSCLKT"
     gene            9342..10637
                     /locus_tag="PLU36_01265"
     CDS             9342..10637
                     /locus_tag="PLU36_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMZ45408.1"
                     /translation="MLTHTPYLLYSDGKGNIFEDTTLFALGRAGWDAFEVELNNWIEL
                     PDGGNLYELPGRKAIGIDTTTGEMRLCEKGWAVAAFIPPAHTSLFLAAYETDKNAPTL
                     PLFCYTAAGWYNNKTYVTAIRIEKDIRQDCAGYNNEKIKNGTDWLLQQYPNNRLVTHL
                     MNTCCMTYSCPAARNFSLNRWECPIPISPACNANCIGCVSFQPTDEDITSPQDRLTFK
                     PTVEEIVEYTVPHLMQAPFPIISFGQGCEGEPLLMWETIVPTIKEIRKHTNKGSININ
                     TNGSKPDAVKKLFESGLDSIRVSLNSARKDIYTKYYRPNNYCFEDIVESLKIAHQYNG
                     WSSINYFVFPGMTDSVEEYEALRKLIKDTNLKMIQWRNFNIDPDWYLGILNISETNEM
                     IGVKQMMHLIQEEFPDLKFGYFNPPIERIKGDFEVNFAH"
     gene            10664..11479
                     /locus_tag="PLU36_01270"
     CDS             10664..11479
                     /locus_tag="PLU36_01270"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HMZ45409.1"
                     /translation="MNLQELIIAAWNDRTLLKDETYTNAVKAVIEEVDKGKLRTASPI
                     AQGNWQVNEWVKQAILLYFGIQQMQTWDVAPFEFYDKMLLKKNYAALGVRAVPHAVAR
                     YGAYLAKNVVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKGVHLSGGVGIGGVLEP
                     LQASPVIIEDGCFIGSRCIVVEGVIVEKEAVLGANVVLTQSTKIIDVSGSEPIEMKGR
                     VPAQSVVIPGSYNKKFAAGEFGVGCALIIGKRKPSTDLKTSLNDALRDFNVSV"
     gene            complement(11558..11749)
                     /locus_tag="PLU36_01275"
     CDS             complement(11558..11749)
                     /locus_tag="PLU36_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45410.1"
                     /translation="MKTKLLRVIGVLAIIMLIALPVIAQDNFPEPEDDIPIDGGLTLL
                     LAAGAGYGAKKLREKRKIK"
     gene            complement(11959..17922)
                     /locus_tag="PLU36_01280"
     CDS             complement(11959..17922)
                     /locus_tag="PLU36_01280"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HMZ45411.1"
                     /translation="MKQLYLPKPFSSIIESKIILLFSFMLFAFVANAQTTIINESCGS
                     SHLHQQMMQNNSNYASKVLANEQRLIKYKNSTYLNKSTSSQTRVVPVVFHVMNDGTPL
                     TQITKEDIKKAIKEMNERFRKIAGSEGDGNGVDVGIEFALAVRDPNGNCTDGINYWDI
                     SGNANFTNYVNNGVSSAPGNPGVPDVTFKDLARWNPSQYYNIWVVGKINGPVDVVAGY
                     ALLADGAHGIDADGTVVQAKYVKFGTTLTHELGHAFNLHHTFQGDGNGGQCPTNVDCE
                     TDGDKVCDTPPHKRSTNCDAGTNSCTGASNEAHVHNYMDYSGGSCQNEFTAGQKDRML
                     FAIDSIRKSFLASEGNMSLVPPAVADADFVNPTTVACVGTNLKFYDKSTCTPNTFLET
                     AFSGISFAWTVKKNGNNVHTSTNQNLSYTFIDTGNYTITLQVTNAHGTDSKTSNLLIT
                     NPAIVAVCQPGSFNQPNNYDATLNNVTFGNINNTTSISKNGAYQDFSCTNATIVDALQ
                     TYPLTISVKSFASSAQRYEAYLDYNNNGIFEYPNELVASGTGVINQNKSYLHNVTIPD
                     SAVKGKIIRLRVMADVHAFSDNKRNCTQVLDLGDVEDYGVYINGTPTPPTVCPPGNVT
                     LTTQAQINSFPINYNNCKQISGNLTITGPSDITNLNGLSNLETITGQLNIVHNNNLTD
                     LSGLSNLKTVGNNFWISDNTALTTINGLNALETTGQFYIGNNAALTTINFSNTLTGLT
                     SFTIVNNPLLQSINGFNNITSISGGLNINNNTVLQNFNAFSKLSSVGNLLQFSDNAQL
                     QNIDSLHKLTTVGGAFNIAKNGALTHLNGFRKLTSVGGFFQLWDNDNLVNIDSLYKLT
                     NVGAELIVEENPQLSNITALAGVNPNTIGGTYGLYIVNNTSLSVCNLPNFCTYLANPS
                     NTHPRTISGNLTNCLNEAAVVAACTAPTNEYVNFLGTMFVQKGASGTGSSWSNAIGEL
                     ADALVIAKSINDTSAGKVKEIWVTSGTYKPMYSPEDGANFGTNQGRNNAFSLLNNVKL
                     YGGFAGSESSFSQRILDTANPTILSGDIDNNNLLDNGNTYHVLVAAGVSSSTVLDGFT
                     IKMGYASSTGSLVVNGNSVERRVGGGIINYSSSPIIRNCIITQNRTDYDGGGMANYFS
                     SNPTITQCFFTGNTASYGGAIENYNNSNPIINNSLFAGNNANYGSGIESYQNSISVIT
                     NCTFSGNSASNAGVIYSYGTSTEPIITNTIIYGNNFGLNYLPTNIISNSLIQGLAGGS
                     NGNLNGNSDPLFVNAPSYILAPFTNGDYRLQIGSPAINTGTSDTTGFNIGNRDVVGIA
                     RIVGGTIDMGAYESYKKFYYTTGSNEGYVLSNWKSNIDGTGSSPSAFKEAADFTIQSG
                     HTITLNNGMDLDVGNNDVIVQPNAVINGNMPLITGGTLTNNGTISTLAILFGNLHQTI
                     KGNGTITNLGLNNSAGATIETNSTVNITGTYTPVLGTLTTNNGLILLSNENGTARIAQ
                     GTDNYINGNVTIERYIPAKAARKWSFITSPVSGTIHNTWQQQIHITGAGTGGTVCPTF
                     TPHINGFDATVSSAPNIYTYDALQISGNRWKVPSSTLSDIIDAGKAFRVNIRGDRSLG
                     CSLLDGSPSGLVPSAVTLRATGALSNAAKNMGSFSVIYPNNGVNNYVFVGNPYPSAIS
                     FSALLTTNNSSINNTYALYVPSSPSGVYSYWDGSTFTGGTGYDNTKGNIIASGQAFFV
                     QSKVAGNLTMFFEESHKTDGANTGYFRTNNYNEKIRVHYLQNDNSQMDEIVIRYANDA
                     TINNVEESSLDIASINSGSNYLTSLKGNKGMAVQTRDLQTHTTDTVWLNVVSTQSGAY
                     KLNFSEFENFATADIYLIDHYTKTTQNVKQNTVYEFSVDKDNAATKGTQRFSVVFNKK
                     QSPELTLNKNIKLYPNPANKQVYVQLPQSAEINYNIKVTNMVGKIVLQQKATNSTEQL
                     NISKLSKGTYIVEIIDSKGNRTTEKLVKN"
     gene            complement(18073..18783)
                     /locus_tag="PLU36_01285"
     CDS             complement(18073..18783)
                     /locus_tag="PLU36_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ45412.1"
                     /translation="MDKKNILLVEDDFLNRRYTKKILIENGYNVLESKNAIETMALLK
                     THTPDLIILDIHLGETDKNGINLAQNIKELFNIPFLFLTAYSATDVVEKAIATLPQSY
                     ITKPFKNIDLITAVELAIRQSNNQEKRKPVVLIKEGGFNIELLVDEIDYIESDGNYLL
                     IYTQEKVYKSRFTIKQILETLPAATFVQTHRAYIVNKNRIEKYSTKNVIVNNKEIPVS
                     KNYIDDIKVFEKMRNLYT"
     gene            complement(18791..>20390)
                     /locus_tag="PLU36_01290"
     CDS             complement(18791..>20390)
                     /locus_tag="PLU36_01290"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HMZ45413.1"
                     /translation="ETAQKITDSIYKGLQSQKNNKALLLEIKYKKALILDRQEKPPNE
                     PLSILLDIIDEVHAAKLHSLLYRIYLLIALCHEKAANLELTNKYLNEAYSIYEKYKLE
                     NLYSMYCIRRGSYYRFLEKIDSVFYFANQAKENAVAYNNEKVLVDSYILLAFAANRNK
                     NYTAALECFFQLKNYRVKYKDTSALAMSYINIAQCYKNMKNFQKALIYSDSSFVFFKS
                     KPTFIYSYQYSKIKYEIFEALGNMDSAYYYLKQYQQEWSIVKNHEVDVKTNELEKQYQ
                     SNKKEAIINSKNQQLFFIIILLFVITVGSILIVRQNRKISKQNKIINNQIDELSKIIE
                     QKQMLLSELQHRVKNNLQHVISILEIQKESVDFNNIDELIRGNQNRIHSMALLHKKLN
                     ISENVNEEDLPKYISELSELVKDSYDNHTKKIQLNVKCEIDKIALEKALPIGLIIVEL
                     VSNSMKYAFLSRSIGIINILLSTNHDTTENTFYYTDNGEGFDFNAIVNKGLGIEIIKG
                     LISQLDGKYETSNSKGFEFMIYFK"
BASE COUNT         6771 a         3287 c         3151 g         7181 t
ORIGIN      
        1 tttacattaa ttgtgttact agaaaatacg ttacttgtgg ttaatgtacc tgaagctgaa
       61 gcagcagtaa atgaagctgg gctatcccct aaagcataat ccacagtcca ggttacagtt
      121 cttccaaccg aattgtctaa agattgtaat aagaattgta attgaacatt atttttaccc
      181 gttgtattat ctaataaaaa tacaaaagaa gctccaggat cataacctgt agaactagtt
      241 tggcgaacac ctaaagctct attgcttgca gcatcttgtg cagtttgatc tgaagttgca
      301 gataaaccag ttgcagaagc aaagttttta aatcctgcac taaaattagc ccaaagtccc
      361 aaagcagaat tgaaagaacc agcagtgcca aaagatgttg ttgtagcccc tgtataaaca
      421 cttacacctg ttggtaaacc agagccaatg ccattaaagt ttaatgtaaa tggtgatgta
      481 gaacccaaag taatacctgt agtgctacca cctcctccag gaccagaacc accagcaaca
      541 acatctgctg catttctaat ggttatttga ggcgttccat tatataaaga aacatatcct
      601 ataaaagtag ttgcatttgc tggtaatgat gaaacaccta aagttgttct aacaaatgaa
      661 acaattgagc cagatgcatc ggttactgta taattttttc cagttgcatt tgatgaggtt
      721 tcagctatgg aaacattagt tagtttaaca actgtagaag accatgaatt gatgtttgag
      781 ttgatttgag agacggttgt atttctaggc acaacagtac cagttgctaa ttttattgca
      841 ttaaccacat tggtaatttg taaatctcca ttgaataaag aaagtgataa gccagaaata
      901 tctacagcaa cactatcatt taatgcaaag tttggattaa tagatgggaa acgtaattgt
      961 aacccataac cactaccatc ttgaatacgg tagttaccat ttgattcgtt taaagtatta
     1021 gatacaatag tacctctaat gcctgtattt gctggcatta tggccccatc tcccattgct
     1081 ctaacatctt gaatagattt ataaactaaa gttgggctta tactgctacc acatctgtca
     1141 tctttaaatt gtacatcgct ggtatctcta attaataatt gtttagtacc attaaaaaaa
     1201 gtatagatag cggttatagt tcctttcttt ttaggtactt gaattccagc aaaattagaa
     1261 tagctacttg ttcttaaagt tatagtatta cctgaacaat tttgaatatt tagatttaca
     1321 gctgaagatg ctaaactagg gtctgcaaaa gtttttaaag tatctgcagc actaaattga
     1381 aagttctcta actgtattaa agtattttga acagtatctt gtaatgttgt ttttaaatcg
     1441 gttatgctag agaaaatttt aggagtaatt acatttccaa atcctccttt aatcacatat
     1501 ttatcaaaca aattttgtgg tattggtgtt tgagaaattg gtgaagtagc tttgttaact
     1561 gcacctaatt gaatcattcc accatactca cccacacaca atcctttaac attaatatat
     1621 actcttcttc ctacagggta gcttgtatat aaattagttc catctaatct taaactaacg
     1681 ccaccagttg catcttgtat agagattgat ttataaaaat ttccgctttt atcatctgca
     1741 actactattc ctgaaatagt tttatctact gtaatttcta caactgttcc tgttgtaggt
     1801 aacaatgctt ttagctcttt taaagtcatt gttattggta agttggggtc aacaaaagtt
     1861 gtatctggtt cgtcaaattt tttattacat gctgcaataa tagtaattgt ggttatcgtt
     1921 aaaaaaaaca atactaacca attgaagtgt ttatttttca tatttctcat ttgtaagagt
     1981 tataataatt ttttactgaa ttttttaatt tattaaaatc taagtgttac actgccaaaa
     2041 taatttaagc cataagcata ataaagctta ggtggaaact tattaatatt tttatcggca
     2101 taatcaaatc tcaactgctc aaagccgcca gagatgatat ttttattgtt taataaatta
     2161 tttataccta ggttaaatac caaataggtt gattttttaa acccaaagct ctttggtaat
     2221 ttccaactcc aaccaccaaa aaagtctata gtataatcgt cttctaattt tgtttgtgct
     2281 aaaatacttt ctctaatagc tgcctcgttt ggcttggtaa tatctaatcc ttccactgca
     2341 tcaaaagttc ttctaatcgg gttaaaatct aaccatctat tagctgcata gttggcactt
     2401 aaactaacaa accaaaattt agggcttcta taagaaaatc ctaagctgaa tgtgttttga
     2461 ggggtgcctg ctaatctata gttttgagaa taaattaaat ctttagttag aacagtagca
     2521 tcattatcta ctgttacagt agcatcttga cggctgttat aataatatct tcctgcggca
     2581 actgctacat ttaatgttac atttctcatt attttagctt ctgcaccaaa ctctaaccca
     2641 aaatgtagtt tatctatgtt actcatagca tagtttacaa aatttctgta atcatcatga
     2701 taaaaagtca ttacattcat ttggttatct attttagtaa aataacctgt aagtctaact
     2761 tttattttag gtgcatttaa cacataacct gcttcggcag tttgaatgct ttcgttcgta
     2821 gtattatctt gaataaaatc tcttgtacgt ggagatatat acacattatc aaacaaagga
     2881 gcccttgtaa gaattgctcc attggcataa atataatttc taccatctaa tttgtatgta
     2941 acaccacctt ttactgcata attattaaaa ctgtttacat ccgattttcc aaatgaatta
     3001 ttagggaaaa ttcctgtttg tgtgtaacca gttctccaaa attgtgtact ggataattct
     3061 cctgcaacaa ataaatcaac tcttttgaat ttgaaaacag attgtaacca agcagaagat
     3121 ttacgtatgg ttatatcgta atcataacca aacttatcgc ctttatataa aattctatta
     3181 ggggtattta aatcattttg attggcagta ttgttattag ggaaagatct ttcggcatat
     3241 tggttaacat tcacataaaa ctcaccacct aataaatctg caacttcttt ataataatta
     3301 tttgtttggc tttgaaaaga aatgccacct gtcatttcta tatttttacc tacacttgta
     3361 ttaaatactg aattaaaatt taaacgtctg gtatcaatta ctctttcttc ttgaatatat
     3421 ctacttcttt tgcctgcaac agtattacct tgaattccat tagcatcttg aatagctgca
     3481 aaactatttc tatttacctc gtacaaacca tgccagttaa tttgcctttt attaatattg
     3541 gtttgcatag cttctaaaac ctgttgttgc aattcgctgc ttaattgata gcttggtaaa
     3601 taacgatagt aatctggtct aggatctggt gcattatacc aatctaaagc actagtactt
     3661 cttttacccc aagttgcact aatagatgta gagattgttg ttctattatt aaaacggtac
     3721 tcgtcgttta aaataaaggt tggctggctg ctttttgcaa tattagcatt gcgttttttg
     3781 ccattttgta aaccccaagt tgggttatag aaattatctc ctgtaattgt tcttaattca
     3841 tccactacac cactttgtct accactttca gttggtgctt caaataaaga caaagacagc
     3901 aaatgctttt gaccaaaacg tttatctacg ccaataaaat aactatatcc gttatagtaa
     3961 gttccaggta catatgcttc atctgcacct ctaaaactac ctgcaaaaac aaatgcccat
     4021 ccttttttgc ttattcctgt accatgataa acggttaaac gatgtgtata atttctattg
     4081 ctaattgcat agcctatttg tgtttgtttt ctttgcttac ttgctctagc atctatagaa
     4141 gaagaagatg caatatcgcc aaaagcaaaa gtgctaggcc ttaagcctaa agatacatct
     4201 ctgttacgca ttacatcatt taaacctccc cataaaccaa atggtgtaaa accattatct
     4261 aaattatcca ttggggttcc attcaaataa gtagtattca aatctccatc atagcccctc
     4321 attttaaatc taaaagcatt aaaattaaaa gacattaccg agaaaaaagg atctctgcca
     4381 gctgtaagta cacttgaaac attttgtgca ccaccatcgc cttgatcatt ttcatcaatg
     4441 gtaactactg caatattatc agacattgca tctttaacat catcggtaat tgttgtatct
     4501 acaacagcac taccattggt tttagcagat ttgttttttt gtttttgtgc cattaccgat
     4561 attggcacag ccattaatag tataatggca aattttaaaa atttcaacat aagtttaatt
     4621 tagtccaaaa ggcaaattta agttcattta agtaataatt agcctctttt ctttaaaagg
     4681 tatttctttc tgtattcagt aagatttata aagaatttat tattttaatt tacttaaagc
     4741 gttttctaat ttttgtagca taattggtat atcttgttta tggcttgtgc caatacttaa
     4801 tctataccag gcactatttt tagaagctcc aaaacaataa aatggtacta ttgctaattt
     4861 ggcttcatta agtaaataag cagttacatc ttcttgtgta tataaaatat ttccttcatt
     4921 agtttgctta ccaactaaat ctatttttac agttaaataa attgcagcct gtggtgtaat
     4981 agcatcaatt tgatatcctt tattttttaa agctataaag ccagcataaa tatgctgcag
     5041 tctataatct atttctttat taaaattgct tacaaattcc tttaatgcag gcgtattaac
     5101 taaatatttt gcagtagctt tttgttctgc catgggtgcc caagcaccaa tatgacttag
     5161 aattgctttc attttagaaa ttacatgctc aggcccaaaa ccccaaccaa ctcttacgcc
     5221 tgtagcacat aacgattttg aaataccacc tacaaaaatg gtatattgct tcattgctgg
     5281 tcttaatgta accggattaa agtgttgaat attatccata gtaagcatcc aatacatttg
     5341 atcaaacatt aagtaaagct ttttttcatg ttctcctctc gagttgtttt cttctataat
     5401 taaatcgcaa attttaccca agctttcctc agaaattgtt gtaccagttg ggttttgagg
     5461 tgtacataaa caaattaaag tggctccttt aatatgcttg gcaacatctt ctggcagtgg
     5521 cataaaatta ttttctggtg tacaatcaat taaacaatgt tgcccattgg ttaaatgtac
     5581 atagtggtta ttattccaac ttggtacgcc ataaattact ttgtctcctt catctacaat
     5641 agctttaaaa atagtataaa taataggtct tcctccacta gcaacctgaa tttctttttc
     5701 attataattt aaatgtaacc attcgtgtaa ataatagcta atagcatttc ttaactctac
     5761 aacaccatct gctggtggat aattagtgct tttatttcta tattcttcta taattagttc
     5821 ttctaataat gttggaatag gaaaaacgga aggattaaaa tcgccaatag tgtaattata
     5881 aatggtttct ccatttctaa tacgttcact aattgcattt cctaatttta caatttcact
     5941 acctaccagt gattctgcaa gtttacttag tttattcata aagccatatt ttggcaacga
     6001 agataagttt ttgaaaaaaa tcaaaaagta aaatactaat atctgctatt tactaattat
     6061 tagattattg gctttcaagc aaaatatttt aattctaaac aaatgttgct aattaaataa
     6121 aacccttttt taaagccaat gctaacctta cttttgcact tcttaaaaaa gaaaaaatat
     6181 gagtaggcaa cttagtgaac aagaaattat aagaagagaa aaacttcaaa aaattaccaa
     6241 tgcaggtata gatgcatttc ctgccccttt atatcctgta aatacctatt ctgcagatat
     6301 caaaaataat tttaccgaag aaaataagga tacttttagt aaggtatgtg tagctggtag
     6361 aattatgagt attaatgata aaggcaaagt tttttttatt aaaatacaag acagtaaagg
     6421 catcattcaa ctatatgtaa aaagagatga gatttgccct gaagaagaca aaagcttttg
     6481 ggataatata attaaacatg gcttagacct tggcgatata attggttgca ccggttatgt
     6541 ttttatcacc aaaacaggcg aaacatctat tcatgcacaa actattacat tactaactaa
     6601 aagcttaaaa cctttacctg ttgtaaaacg agatgaggaa ggtaatgtat ttgatgaagt
     6661 tactgaccca gaatttagat acagacaacg ttacgcagac ttaattataa acccatcggt
     6721 taaagaaaca tttacaaagc gtactaaact tgtaaatact atcagagaat atttaaataa
     6781 tttaggtgca ttagaagtag atactcctgt tttacaagca atacctggtg gtgcagcagc
     6841 tagaccattt attacacacc ataatgcttt agatgtacct ttttatttaa gaattgccaa
     6901 tgaattgtat ttaaaaagat taattgtggg tggctttgat tgggtttatg agtttagcag
     6961 aaattttaga aacgaaggca tggatagaac gcataatcca gaatttacca ttttagaatg
     7021 gtacactgcg tataaagatt atttctggat gatggaaata acagaacaac ttttagaaaa
     7081 gattgcagtt gctttaaatg gtactactac attacaagtt ggcgacaaaa caattagctt
     7141 taaagcacct tttagaagaa tttctatttt tgatgccata aaagaaaaca caggtattga
     7201 tatttctgaa aaagatgaaa ttgggttaag agaagtatgt aaacaattaa atattgatgt
     7261 gccgcctact attggtaaag gaaaactaat agatgaaatt tttggtgcta ctagcgaaca
     7321 tacttatatt caaccaacct ttattattga ttaccccgta gagatgagtc cacttaccaa
     7381 aaaacataga agtaaaaaag gattagtgga aagatttgag ttaatgatta atggcaaaga
     7441 aattgctaat gcatatagcg aattaaacga cccaattgac caacgtgaac gttttgaaga
     7501 acaagctaaa ctaatggaac gtggcgatga tgaagcaatg tttattgatt atgatttttt
     7561 aagagcatta gagtatggta tgccaccaac aagtggtatt ggtattggta ttgatagatt
     7621 agtaatgtta atgactaatc agcagagcat tcaagatgtt ttattctttc cgcaaatgag
     7681 gccagaaaaa caagaagaat aattattatt taaaaagtaa taaaatagaa ttaacaaaag
     7741 ttgtataaaa aaagattatg tcagatttat caaaatttaa tccaaactct ttaggtaatc
     7801 ctaacaacaa tatttttggt ttgccattta ccgaagagga agcatcgctt atacttttac
     7861 ctgtaccttg ggaggttact gtaagttatg gagcaggtac tgcaagatcg gcagaacata
     7921 tttttaatgc tgcattgcag gtagatttgt ttgatgtaga tgcacccaat gtttggaaga
     7981 aagggtttta catgctacca gttgataaaa agcttttaat gaaaagcgac tatttgcgta
     8041 aagaagcaga attgtatatt gattttatag caaaagaaga aaactttgaa ggcaataaat
     8101 ttatgtgtaa aaaccttaaa gaaattaatg aaggttgtaa ttacatgaac aattttgttt
     8161 atcaacaaac caaacaactt ttaggtaata ataaatttgt tggcttaatt ggcggagacc
     8221 acagtaccac ttttggatac atcaaatcac tctcagaaaa atacaaccaa tttggtattt
     8281 tgcaaataga tgcccactgc gatttaagag ttgcctacga aaactttacg tacagccacg
     8341 caagtatcat gtataatata ttatcagagt ttgaacaagt taacaagtta gttcaattag
     8401 gtattagaga ttatagtgat gatgaataca attacattaa aaacagtaat ggcagagtaa
     8461 ccacattttt tgatagagat attaaacaca gaatgtacga aggagaaatt tggaaaaata
     8521 ttgtatctga aatagtagat gccttgccta ataatattta tataagtttt gatgtagatg
     8581 gtttagaccc caaactttgc ccaaatacag gaacgcctgt agcaggaggt tttgaaacag
     8641 aacagattgt ttacttattt aataaattaa aagaaagtaa taaaaagata attggttttg
     8701 acttggtaga aacaggagtg ggcgaaaatg attgggatag caatgtatct gcaaggcttt
     8761 tatggaaaat gtgcaattta gtaatgaacc aataattagt agaatatgca atttaatttt
     8821 tctgttttta ataaccgaaa aaaacaaatt tggttattgg cttacggatt aatgatagtt
     8881 aatggattaa tttttttgct gtttgcccta aagagtaata tttataaatt agcttttatc
     8941 tattctgcat tactaataac tactacactt gtaagcatct tcataaaaaa atacaaatca
     9001 attttctaca acgtttccta tttacttata gcttttagtt ggatacaact taattatcca
     9061 tggattggag tattgctaat tattttacta tcagttacaa aaaagtttac agccgatatt
     9121 gccgttacaa ttaatgagaa agaggttgaa atcaaaaatg tattctctaa taattatcaa
     9181 tggagcagtt ttcaaaatgt aatacttaaa gacaaattac ttactttaga tttcaaaaat
     9241 aataaaatac ttcaggtcga aattcttaat aaaactacac tagagcaaga aaaagaattt
     9301 aacgaatttt gccaaagttg tttaaaaact taacacagtt tatgcttacc cacacaccat
     9361 accttttata ctctgatggt aaaggaaata tatttgaaga tacaacattg tttgctttag
     9421 gaagagcagg atgggatgca tttgaagtgg agttaaataa ttggatagaa ttaccagatg
     9481 gtggaaattt atatgaacta ccaggtagaa aagctattgg cattgatacc acaacaggcg
     9541 aaatgcgttt gtgcgaaaag ggttgggctg ttgcagcatt tattccccct gcacacacca
     9601 gtttattttt agcagcttat gaaacagata aaaatgcccc aactttgcct ttgttttgtt
     9661 atactgctgc tggttggtat aacaataaaa cttatgtaac cgcaataaga atagaaaaag
     9721 acatacgcca agattgtgct ggatataaca acgaaaaaat aaaaaatgga accgattggc
     9781 ttcttcaaca atatccaaat aacagattgg taactcattt aatgaacact tgctgtatga
     9841 cttatagttg ccctgcagca agaaactttt ccttgaacag atgggaatgt ccaattccaa
     9901 tttcaccagc ctgtaatgcc aattgtattg gctgtgtatc ctttcaacca acagatgaag
     9961 atataacttc gccacaagat agattaactt ttaaacctac tgtagaagaa atagtagaat
    10021 atacagtacc acatttaatg caagcacctt ttcctataat aagctttggg caaggctgcg
    10081 aaggtgagcc tttattaatg tgggaaacga ttgtacctac tataaaagaa ataagaaaac
    10141 acaccaataa aggaagcata aatattaata ccaacggctc taaaccagat gctgtaaaaa
    10201 aattatttga atctgggtta gatagtatta gagtgagttt aaacagtgcc agaaaagata
    10261 tttacacaaa atattatcgc cccaataatt attgttttga agatattgtt gaaagcctaa
    10321 aaattgctca tcaatacaat ggttggagta gtattaatta ttttgttttt cctggaatga
    10381 cagatagtgt ggaagaatat gaagctctta gaaaattaat aaaagatacc aatttaaaaa
    10441 tgatacaatg gagaaatttt aatatagatc cagattggta tttaggcata ttaaatattt
    10501 cagaaactaa tgaaatgatt ggagtaaaac aaatgatgca tttaattcaa gaagaatttc
    10561 ctgatttaaa atttggatat tttaatccac caatagaaag gataaaaggg gattttgaag
    10621 tgaactttgc acactaaaaa ttattttaat aaaaaaaaca gaaatgaatt tacaagaact
    10681 aattatagca gcctggaacg atagaacttt attaaaagat gaaacttata ccaatgcagt
    10741 aaaagcagta attgaagaag ttgataaagg aaaattaaga acagcttcac caatagctca
    10801 aggtaattgg caagtaaatg aatgggtaaa acaagctata cttttatatt ttggtattca
    10861 acaaatgcaa acatgggacg ttgctccttt tgagttttac gataaaatgc tattgaaaaa
    10921 aaattatgca gcattaggtg taagagcagt gccacatgca gttgcaagat atggtgctta
    10981 cttagctaaa aatgttgtgc ttatgccaag ctatgtaaat attggggctt atgtagatga
    11041 aggcacaatg gttgatactt gggcaactgt gggtagttgt gctcaaattg gtaaaggtgt
    11101 tcatttaagt ggtggtgttg gtattggtgg tgttttagag cctttacaag caagccctgt
    11161 tattatagaa gatggttgtt ttattggtag ccgttgtatt gttgtagaag gtgtaattgt
    11221 agaaaaagaa gccgtattag gtgctaatgt tgttctcact caatctacaa aaattattga
    11281 tgttagtggt agcgagccaa tagaaatgaa aggacgtgta cctgcacaaa gtgttgttat
    11341 tcctggttct tacaataaaa aatttgctgc aggagaattt ggggtaggtt gtgcattaat
    11401 tattggtaaa cgaaaaccaa gtaccgattt aaaaacaagc ctaaatgatg ctttgagaga
    11461 ttttaatgta agtgtgtaaa tattcatttt cttcagaaaa ttttatttaa caaaaacaaa
    11521 accccgaatg ttataatgga ttcggggttt tgttttttta tttaattttt cttttctccc
    11581 ttaatttttt agcaccataa ccagcacctg ctgcaagcaa taaagttaat cctccatcta
    11641 ttggaatatc atcttctggc tctggaaaat tatcttgtgc tattacaggc aatgctatta
    11701 gcattatgat tgccagcact cctattactc ttaataattt tgttttcata attattttat
    11761 tttttaatgt tctttctgaa tgaaataaaa agcaatgtca tgttgaacga agtgaaacat
    11821 ctgcttgatc ctttaatgct attctgaaga tccttcgctt cgctcaggat gacataaaga
    11881 actactcagg ataacaacga tttatcattc catttcttaa ctcttaattc ttaacactaa
    11941 actcataatt ctaaactctt aattctttac caatttctct gttgttctgt tgcctttgct
    12001 gtctattatt tctacaatat aagttccttt gcttagctta cttatgttta gctgttctgt
    12061 actgtttgtt gctttctgct gtaatactat tttgcctacc atatttgtta ctttgatgtt
    12121 gtaattaatt tctgcacttt gtggtagttg tacatatact tgtttgtttg caggattagg
    12181 atatagttta atattcttgt ttaaagtcaa ttcgggtgat tgctttttgt tgaataccac
    12241 actaaacctt tgtgttcctt tggtagcagc attatcctta tcaacactaa actcatacac
    12301 agtattttgt tttacatttt gtgttgtttt agtgtaatga tcaattaaat aaatatctgc
    12361 tgttgcaaag ttttcaaact cgctaaagtt taatttgtaa gctccacttt gtgtacttac
    12421 tacattcaac caaactgtat ctgtagtatg tgtttgtaag tctcttgttt gtacagccat
    12481 gcctttgttt cctttaaggc ttgttaggta attgctacca ctatttatag aagcaatgtc
    12541 taaactgctt tcctctacat tattaattgt tgcatcattg gcataacgaa ttacaatttc
    12601 atccatttgt gaattgtcat tttgcaaata atgtactctt attttttcat tgtaattgtt
    12661 tgtacggaaa taacctgtat tggcaccatc tgttttatga ctttcttcaa aaaacattgt
    12721 tagatttcct gctactttag attgtacaaa aaatgcctga ccgcttgcta taatatttcc
    12781 tttagtatta tcatatcctg tacctcctgt aaaagttgaa ccatcccaat aactgtacac
    12841 tccagaaggg cttgaaggca catacaaggc ataagtatta ttaatactac tattattggt
    12901 tgttagcaat gcactaaagc taattgcact tggataagga ttccctacaa atacataatt
    12961 attaacgcca ttgttaggat aaatgacact aaagctgccc atatttttcg cagcattgct
    13021 caaagctcct gtagctctta atgttacagc acttggtact aaaccacttg ggcttccatc
    13081 taacaaacta catcctaaac tcctatctcc acgaatattt actctaaatg ctttacctgc
    13141 atctatgatg tctgacaaag tacttgatgg tactttccaa cggttaccac ttatttgtaa
    13201 agcatcataa gtataaatat tgggagcact gctaactgtt gcatcaaaac cattaatatg
    13261 tggtgtaaat gtagggcaaa ctgtgcctcc tgtacctgca cctgtaatat gtatttgctg
    13321 ttgccatgta ttgtgtatag taccacttac agggcttgtt ataaaactcc atttacgtgc
    13381 tgctttggca ggtatgtaac gctctattgt aacgttgcca ttaatgtaat tgtctgtacc
    13441 ttgagctata cgagcagtac cattttcatt gcttaataaa attaaaccgt tattggtagt
    13501 taaagttcct aatacaggtg tataagtgcc tgttatattt acagtgctat ttgtttcaat
    13561 ggtagcacct gcagaattat taagacctaa attagttatt gttccattac cttttatagt
    13621 ttgatgtaga tttccaaata gtatagctaa tgttgagata gttccattat tagttaaagt
    13681 tccaccagta attaaaggca tgttaccatt aataactgca ttgggctgaa caattacatc
    13741 attgtttcca acatccaaat ccattccatt atttaaagtt attgtatgac cagattgaat
    13801 agtaaaatct gcagcttctt taaatgcaga aggtgatgaa cctgttccat ctatattgct
    13861 tttccagttg cttaaaacat atccttcatt actacctgtt gtataataga attttttgta
    13921 agattcgtaa gcacccatat ctattgtacc acctactatt cgtgcaatac ctacaacatc
    13981 tctattacca atattaaaac ctgttgtatc agaagtacca gtattaatag ctgggcttcc
    14041 tatttgtaat ctataatcac catttgtaaa aggtgctaaa atatatgaag gagcatttac
    14101 aaacaatgga tcagaattgc catttaaatt accatttgaa ccaccagcta acccttgaat
    14161 taaactatta ctaataatat tggtaggtag ataatttaac ccaaaattat taccataaat
    14221 aatggtattg gttattatag gttctgtaga tgttccataa gaataaatta ctccagcatt
    14281 acttgcacta ttgccagaaa aagtacagtt tgtaattacg gatattgaat tttgataact
    14341 ttctattcca ctgccataat ttgcattatt ccctgcaaat aggctattgt ttataatagg
    14401 atttgagtta ttatagtttt caatagcacc tccataagat gcagtattac ctgtaaaaaa
    14461 acattgagta attgttgggt tggatgaaaa ataattagcc atacctcctc catcataatc
    14521 agttctattt tgtgtaatta tacagtttct aattatagga gaagatgagt agttaattat
    14581 tccaccacca actcttcttt caacagaatt accatttaca actaaagacc cagtagaact
    14641 tgcatacccc attttaatag taaaaccatc taacacagta gagctggata ctcctgcagc
    14701 aactaaaaca tggtatgtat taccattatc cagtaaatta ttattatcta tatctccact
    14761 taaaatagtt ggattagcag tgtctaaaat tctttgagaa aatgaagatt cagaacctgc
    14821 aaaaccgcca tataatttta cattattcaa taaagaaaaa gcgttgtttc tcccttgatt
    14881 tgttccaaaa ttagcaccat cttctgggct atacataggt ttataagtac cacttgtaac
    14941 ccatatttct tttacttttc cagcacttgt atcattaatg ctttttgcaa ttaccaatgc
    15001 atctgccaac tcaccaatag cattactcca actgcttcct gtaccagatg ctcctttttg
    15061 tacaaacatg gtacctaaaa aattcacata ctcatttgta ggtgcagtgc aagctgctac
    15121 aactgctgct tcatttaaac aattcgtaag attacctgaa attgttcttg gatgagtatt
    15181 ggaagggttt gccaagtaag tacaaaaatt aggaagatta catacagaaa gacttgtatt
    15241 atttactata tataaaccat acgtgccgcc tatagtatta gggtttactc ctgcaagagc
    15301 tgtaatattt gacaactgtg gattttcctc tacaataagc tcagcaccaa cattagtgag
    15361 tttataaagg ctgtctatat ttaccagatt gtcattatcc catagttgaa aaaatccacc
    15421 aacagaagta agttttctaa agccattaag atgcgttaat gctccatttt tagcaatatt
    15481 aaaagcacca ccaacagtag taagtttatg taaactgtct atattttgaa gttgagcatt
    15541 gtctgaaaat tgtaataaat taccaacaga actgagttta ctgaaagcat taaaattttg
    15601 cagaactgta ttgttattaa tatttaaacc gccgctaata cttgttatat tgttgaatcc
    15661 atttatactt tgcagtaagg gattatttac aatggtaaaa ctagtgagac ctgttaatgt
    15721 gttagaaaaa ttaattgtag ttaaagctgc attatttcca atataaaatt gccctgttgt
    15781 ttctaaagca ttcaaaccat taattgtagt taatgctgta ttatctgaaa tccaaaaatt
    15841 atttccaact gtttttaaat tggataatcc acttaggtca gttaagttat tgttatgtac
    15901 aatattaagt tgtcctgtaa tcgtttctaa attacttaaa ccattaagat ttgtaatatc
    15961 tgaaggtcct gtaatagtta agttgccact tatttgtttg caattattat aattaatagg
    16021 aaagctatta atttgtgctt gagtagttaa agttacattt ccaggagggc atacagttgg
    16081 tggtgttgga gttccattaa tataaacacc ataatcttct acatctccta aatctagaac
    16141 ttgtgtacag ttgcgtttat tatcactaaa tgcatgtaca tcagccataa ctcttaaacg
    16201 aataattttt cctttaacag cagaatctgg aattgtaaca ttgtgtaaat agcttttatt
    16261 ttgattgata actccagtac cagaagcaac taattcatta ggatattcga atatgccatt
    16321 attgttataa tctaaataag cctcataacg ttgtgcagaa cttgcaaacg atttaactga
    16381 aatggttaat ggataggttt gtaatgcatc cacaatggta gcatttgtac atgaaaaatc
    16441 ctgataagct ccattttttg aaatggatgt agtgttatta atattcccaa aagttacatt
    16501 atttagtgta gcatcatagt tgttaggttg gttgaatgag ccaggttgac aaactgcaac
    16561 aattgctgga ttggttatta agagattact tgttttagaa tcagttccat gagcattggt
    16621 aacttgcaag gtaattgtat aattaccagt atctataaaa gtataagata gattttgatt
    16681 tgtagaagta tgtacgttat taccattttt tttcactgtc catgcaaatg aaatacctga
    16741 aaatgctgtt tcaagaaatg tattaggtgt acaagtagat ttatcataaa acttcaaatt
    16801 tgtaccaaca caggcaactg ttgtaggatt aacgaaatca gcatcagcaa cagcaggagg
    16861 cacaagcgac atatttcctt cggaagctaa aaaagatttc cgaatactat caattgcaaa
    16921 taacattcta tctttttgcc ctgcagtaaa ttcattttga caagaaccac ccgaataatc
    16981 catatagttg tgtacatgag cttcattaga agctcctgta caactattgg tgcctgcatc
    17041 acaattagtt gaacgtttgt gtggtggtgt atcgcatact ttatctccat ctgtttcaca
    17101 atctacattc gtagggcatt gacctccatt accatctcct tgaaaagtat gatgtaggtt
    17161 aaaagcatgc cctaattcgt gtgtaagtgt tgtaccaaat tttacatatt ttgcttgtac
    17221 tacagtgcca tcagcatcaa ttccatgagc accatctgca agcaaagcat aaccagccac
    17281 aacatctaca ggtccattta ttttacctac tacccaaatg ttgtaatatt gtgatggatt
    17341 ccatcttgcc aaatctttaa aagtgacatc tggcactcca ggattaccag gggcagaact
    17401 aacaccatta ttaacataat tagtaaaatt tgcattacca gaaatatccc aataattaat
    17461 tccatctgta caatttccat tagggtctct aacagcaagt gcaaattcta ttcctacgtc
    17521 tacaccatta ccatctcctt cagatcctgc aatttttcta aagcgttcat tcatctcttt
    17581 aattgctttc ttaatgtctt ctttggttat ttgtgttaat ggtgtaccat cattcattac
    17641 atgaaaaaca acaggcacta ctcttgtttg tgaggatgta ctcttgttta agtaggttga
    17701 atttttgtac ttaattaacc tttgttcgtt ggctaacact tttgatgcat aattagaatt
    17761 gttctgcatc atttgttgat gtaaatgaga tgagccacac gactcattaa taattgttgt
    17821 ttgtgcattt gcaacaaaag caaatagcat aaaactaaac aataaaataa ttttagattc
    17881 gataatagag ctaaaaggtt ttggtaaata cagttgtttc atgtggtaaa atgtttatga
    17941 aacaaaaata tttgtctggt atctatacta aatttttatg ggataaaaca aagatatttg
    18001 ggatagaaaa tacattccta aaattactct attaaaattg caaatgttat aatagaaaat
    18061 tagcttggct ttttaagtat ataagtttct cattttttca aaaactttta tatcatcaat
    18121 atagtttttc gaaacaggaa tttctttatt attcactatt acatttttag tactatattt
    18181 ttcaattctg tttttattta caatgtacgc tctatgtgtt tgtacaaaag ttgcagcagg
    18241 aagtgtttct aaaatttgtt taatggtgaa acggcttttg tacacttttt cttgagtata
    18301 aattaaaaga taatttccat cagattctat gtaatctatt tcatcaacaa gtagttcaat
    18361 attaaaccct ccttctttta ttaatacaac aggtttacgt ttttcctgat tgttagattg
    18421 gcgaattgca agttctactg ctgttatcaa atctatattt ttaaatggct tggtgatata
    18481 ggattgtggc aaagtagcaa ttgctttttc aacaacatca gtggcactgt aggctgtaag
    18541 gaacaaaaaa ggaatattaa atagctcttt aatattttgt gcaaggttta tcccattctt
    18601 atctgtttct ccaaggtgaa tatcaagaat aataaggtct ggtgtatgtg tttttaataa
    18661 tgccattgtt tcaatagcat ttttactttc caatacatta tatccgtttt ctattagtat
    18721 ttttttggta tatcttctat tcaaaaaatc atcctctact aataaaatat tttttttgtc
    18781 catcttgcca ttatttaaaa taaatcataa actcaaaacc tttactgttt gatgtttcat
    18841 attttccatc taattgacta attaaacctt ttattatttc aatgcctaag cctttattta
    18901 ctattgcatt aaaatcgaaa ccttcgccgt tatcagtata ataaaatgta ttttctgttg
    18961 tatcgtgatt ggttgatagc aaaatattaa taataccaat gcttcggctt aaaaaagcat
    19021 atttcatact attgctaact aattccacaa taattaaacc tattggtaat gctttctcta
    19081 aagctatttt atcaatttca catttaacat ttaattgaat tttctttgta tgattatcgt
    19141 aggaatcttt taccaattcc gaaagctctg aaatgtattt tggcaaatct tcttcattca
    19201 cattctctga tatattcaac tttttatgca acaatgccat agagtgaata cggttttgat
    19261 tgcctctaat gagttcatca atattattaa aatctacaga ttccttttgt atttccagaa
    19321 tgctaataac atgttgtaaa ttatttttta ccctatgttg caactcagag agaagcattt
    19381 gcttttgttc tataatttta gaaagttcgt ctatttgatt attgattatt ttattttgtt
    19441 tactgatttt tctattttgt ctaacaatta aaatagaacc aactgtaatt acaaaaagca
    19501 ggatgatgat gaaaaaaagt tgttgatttt tgctgtttat aatggcttct ttcttattgc
    19561 tttggtattg tttttcaagc tcatttgttt taacatctac ttcatgattt tttactatac
    19621 tccattcctg ttgatattgt tttagataat aataagccga atccatatta cctaatgctt
    19681 caaaaatttc atactttatt tttgaatatt gataactata aatgaaggtg ggcttgcttt
    19741 taaaaaaaac aaaagaggaa tcactataga tcaaagcttt ttgaaaattt ttcatgtttt
    19801 tatagcattg agcaatgttg atgtatgaca ttgctaatgc agacgtgtct ttatatttta
    19861 ccctataatt ttttaactga aaaaaacatt ccaatgcagc cgtataattt ttatttctgt
    19921 tagcagcgaa tgcaagtaat atataagaat caaccaaaac tttttcatta ttgtatgcta
    19981 ctgcattttc ttttgcttga ttggcgaaat aaaaaactga atcaattttt tctaaaaatc
    20041 tataataaga acctcgtcta atacaataca tgctgtacaa gttttcaagc ttatactttt
    20101 cgtatatact atacgcttca ttaagatatt tattggttag ctctaagttg gctgcttttt
    20161 cgtggcacaa agcaattaac aaataaatac ggtataagag tgaatgaagt tttgcagcat
    20221 gtacttcatc tataatatcc aataatatgc ttagaggttc gttagggggt ttttcttgtc
    20281 tgtctaatat aagagccttt ttgtatttta tctctaataa taaggcttta ttattttttt
    20341 gcgattgtag tcctttgtaa atactatctg ttattttttg agcagtttca
//
LOCUS       DAOQPK010000013        19532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_1390_length_19532_cov_4.537095, whole genome shotgun sequence.
ACCESSION   DAOQPK010000013 DAOQPK010000000
VERSION     DAOQPK010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 19532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19532
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1390_length_19532_cov_4.537095"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PLU36_01295"
     CDS             complement(<1..247)
                     /locus_tag="PLU36_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HMZ45414.1"
                     /translation="MKILMVCLGNICRSPLAEGILRTKANKLNLNWQVDSAGTADYHV
                     GKPPHILSQKVALENGIHIGAHKCRQFNKNDMLLFDKI"
     gene            440..670
                     /locus_tag="PLU36_01300"
     CDS             440..670
                     /locus_tag="PLU36_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45415.1"
                     /translation="MNSNEDKRRKSYLLMRMTYDIGMAVFLIGMAVFLLLGNFWGIEQ
                     ILTIDSTFRYMFGSICLLYGLFRLYRGIKREY"
     gene            672..1592
                     /locus_tag="PLU36_01305"
     CDS             672..1592
                     /locus_tag="PLU36_01305"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HMZ45416.1"
                     /translation="MKKYLIIALCCLFVIMQACNNATTYNLNNDTPQTGKIFISVDES
                     FKPVIEQQIKVFQSSYPKANIIAEYKPEVECFKDLQEDSTRMILVARNINDKETEYFN
                     AKLSYTPKQDILAYDAVVVIVNINSKDSIFTLQQLQDILNGKNNITAVMDGSNATSTV
                     RFLKDSILKGEQFGKNVVASNGSNAVVEAIAKMPNAVGFVGLSWVGNTDEQNQKEQLN
                     KIKLALIECVKCNDKGVFAKPSQSTITYGQYPLARPLYYVLKEYSEGLGTGFKNFMTN
                     ERGQLIFRRAYLAPAKMNLNTRNSAIKEEQ"
     gene            1641..3302
                     /locus_tag="PLU36_01310"
     CDS             1641..3302
                     /locus_tag="PLU36_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45417.1"
                     /translation="MMKKIATIIFTLMLVWSGINAQSIEDGKKLLSYQKNKSAVDLFQ
                     QLYNKNTNSAEHIYWYGQSLIANKNYAEAKALYQKALQAGVNEPLIWIGAGHIELIEK
                     GDLNSAKQKFEQAITASTPTKGRNKHKPDPSILNAIGRANAYGDSKMGDPNYGIEKLR
                     QASEIDLKDAEILVNMGKCYQKLGGEMGGDAVKAYTEATVRDPKYAEANWRMGLIYYS
                     QDNKDMYEKYYNAAIASDIEFPPVYLSYYRVYEQTNVNVAKEYLDKYMQYADKDCGTD
                     YFYADYLFRAGKYQESLNKAIEMSNSNCKNFERLPILFAYNYDRLGDSINAKKFIDQF
                     FATTNTADIEATDYELAVKVFSKFEGVENQTVAFLQKAIDNDSKKENKLKYTKMAADI
                     YGKAKMYKEHVQWLHKYNDLKGSMGEYDYYVITNSSFQSKDYVGTMVLAQKYIAAFPN
                     KPQGYAYNVAAAKALDTSSTICFAEPAILQYNDFLLKDSTTNKSQIVSNYYYIMIYYA
                     DKIKDYSKALEYCNKILLIIPDDKEMLDIKKVLEERAAKSPKSTK"
     gene            3433..3828
                     /locus_tag="PLU36_01315"
     CDS             3433..3828
                     /locus_tag="PLU36_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HMZ45418.1"
                     /translation="MHHFLLNASNANTTSNYLPIGIQLIFAVGFVATMLFLSAMVGPK
                     RRTKEKLETFTSGIESHGDARQPVSVKYFLVAILFVLFDVEVIFFYPYAVNFKMLGWE
                     GFLAVLMFVALFLVGFIYIIKKKALKWED"
     gene            3895..4482
                     /locus_tag="PLU36_01320"
     CDS             3895..4482
                     /locus_tag="PLU36_01320"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HMZ45419.1"
                     /translation="MRPVKYNIHPKEAKLADSLIVSEMPDGYTGEGFFATKLSDVVGL
                     ARKNSIWPMPFATSCCGIEFMATMASHYDLARFGSERVGFSPRQCDLLMVMGTIAKKM
                     GPVLRQVYIQMAEPRWVMAVGACASTGGIFDTYSVLQGIDQIIPVDVYVPGCAPRPEA
                     ILDGVMRIQDIVQNESIRRRNTDHYKALLNSYGIE"
     gene            4506..5027
                     /locus_tag="PLU36_01325"
     CDS             4506..5027
                     /locus_tag="PLU36_01325"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HMZ45420.1"
                     /translation="MMALNNEIVKQKLVEKFGEQLTNWEEPYNFLTFVAPKDLNLKVL
                     QFLYDDETLKFRFLTDLCAVHYPDRKGEEVAVVYHLHNLENNIRVRFKVYTDAEKTEV
                     YTATALYEAANWMERETYDFFGVNFIGHPNLKRILNVDEMDYFPLRKQYPLEDQTRVD
                     KDDEMFGRGGSIL"
     gene            5069..6301
                     /locus_tag="PLU36_01330"
     CDS             5069..6301
                     /locus_tag="PLU36_01330"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HMZ45421.1"
                     /translation="MSDILHHNIKLPKGAIEKETTTLNLGPTHPATHGVMQNIIELDG
                     ERIVSAEQTIGYIHRAFEKLAERRPLFQITTLTDRLNYCSAPLNNMGWHLTCEKLLGI
                     QTPKRVDYLRVIIMELSRITDHLICNSIVGVDSGAFTGFLYVMQYRELVYEIYEEICG
                     ARLTTNMGRIGGFERNFNDTAWKKLDKFLVEFPKAWKEFGNLFLRNRIFMDRLIGTGA
                     ISAERALNYGFTGPNLRAAGVDYDVRVHTPYSSYQDFNFTIPVGTTGDNYDRFLVRDQ
                     EVWQSISIIQQAYQKVQEFKGAEAEVYHADAPEYYLPEKELVYTRMESLIWHFKIVMG
                     ETEIPKGEVYNSIEGANGELGFYLISDGGRTPYRLHFRRPCFIYYQAYSELTKGGMLS
                     DAIMTMSSLNLIAGELDA"
     gene            6373..6873
                     /locus_tag="PLU36_01335"
     CDS             6373..6873
                     /locus_tag="PLU36_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HMZ45422.1"
                     /translation="MIQFNDTQMAEFKRLVNRYPADKKKSALLPVLHLAQDSFGGWLS
                     TETMDYVAALLEIKPIEVYEVATFYSMYNLKPVGKYMFEVCQTGPCMLRGSDDIIAYI
                     NEKLGIKPGETTADGMFTLKTVECLGACGYAPMMQLGKHYKEHLTKQKIDEIIEDCKK
                     AAINNN"
     gene            6990..8345
                     /gene="nuoF"
                     /locus_tag="PLU36_01340"
     CDS             6990..8345
                     /gene="nuoF"
                     /locus_tag="PLU36_01340"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HMZ45423.1"
                     /translation="MGSIKLLLEKAHVEGIRYYDVYRREGGYRSVEKALKTMSPETIV
                     EEVKKSGLRGRGGAGFPAGMKWSFIAKPEGVPRHLVCNADESEPGTFKDRYLMEFIPH
                     LLIEGLIVSSFALGSNDTYIYIRGEYAWIVDILEQAIIEAKNNGWLGKNILGSGFDCN
                     IHVQRGAGAYICGEETALIESLEGKRGNPRIKPPFPAVQGLWMRPTVVNNVETLAAVV
                     PIINMGGEEYAKIGVGRSTGTKLISACGNINKPGVYEIDMTISVEEFIYSDEYCGGIA
                     NGKRLKACIPGGSSVPILPANLLLKTAKGETRFMNYESLSDGGFASGSMMGSGGFIVL
                     DEDQCVVKNTYTLARFYRHESCGQCSPCREGTGWMEKILWNIESGKGKMSDIDLLWDV
                     QSKIEGNTICPLGDAAAWPVAAAIRHFRDEFEWHITNKEEAQKRNFGLAHYANPIHVP
                     A"
     gene            8461..9558
                     /locus_tag="PLU36_01345"
     CDS             8461..9558
                     /locus_tag="PLU36_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HMZ45424.1"
                     /translation="MSETLFKVTIDNIVIEVPAGTTILNAARKIGGEVAPPTMCYYSK
                     LEGSGGKCRTCLVEVSKGSEKDPRPMPKLVASCRTTVMDGMEVKNITSERVLTARAGV
                     VEFLLINHPLDCPICDQAGECYLQDLSYEHGADKSRFEFQKRTFKKVDLGDKIQLHMT
                     RCILCYRCVLVANQLTNQREHGILDRGDHAQIATYIQKNLQNDFIGNIIDVCPVGALT
                     DKTFRFKNRVWFTKPVDAHRDCNNPKCCGEVRLWMRGDEVYRVTGRIDEWGEVKDASN
                     GKPGWICNECRFDKKKVSDWVIEGPTNVNRHSVISQGHYVNSVLPQETLPEVLNGRQP
                     KLLMDIHNVSEVNKPNIELSALDRPAHSTDF"
     gene            9578..10642
                     /gene="nuoH"
                     /locus_tag="PLU36_01350"
     CDS             9578..10642
                     /gene="nuoH"
                     /locus_tag="PLU36_01350"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HMZ45425.1"
                     /translation="MTFLTIDWLFIIEKLVLIIIIILASLGIALYTTFAERKVAAILQ
                     DRPGPNRAGPFGLLQPLADGVKMFTKEEIVPTHASKWLFILGPGLAMVASLMTCAVIP
                     WGGSVELFDRKIDLQVADINIAVLYIFAVVSLGIYGIMIGGWASNNKFSLMAVLRAAS
                     QAISYELAMGLSLIAVLMITGTLSLNEMVKQQQEGLWNIVYQPLGFLLFFICALAECN
                     RTPFDLPEAESELNFGYHQEYSSMKLGFYLFAEYINMIMSSAVMATLYFGGYDIPFVN
                     DANLYASIGNWAALLQVTSILIKIVIFIFVFMWIRWTTPRFRYDQLMDLGWKKMVPLA
                     LFNMLVTAFIVLLKHNGWKF"
     gene            10904..11389
                     /gene="nuoI"
                     /locus_tag="PLU36_01355"
     CDS             10904..11389
                     /gene="nuoI"
                     /locus_tag="PLU36_01355"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HMZ45426.1"
                     /translation="MNFLERIYLWNIFKGMFITFKHMFKKQPTIHYPEQKRTFSPVFR
                     GLHVLNRDEEGRERCTACGLCAVACPAEAITMEAAERLPEEENLYREEKYAAKYEINM
                     LRCIFCGLCEEACPKDAIYLSETFAPSNYSRKNFIYSKQDLLIPNPKTETADLEKAKG
                     K"
     gene            11401..11916
                     /locus_tag="PLU36_01360"
     CDS             11401..11916
                     /locus_tag="PLU36_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HMZ45427.1"
                     /translation="MNLIQILFWFLTGLALFSALMVILSKNPVHSVLWLIVVFFAISG
                     HYILLNAQFLAIVNLIVYAGAIMVLFLFVIMLLNLNKETEPQKNVWMKLAGTIAGGCF
                     LLSVCNVIYNAQVTTLELNKKTVLMGEGNIGLIENLGKKLFTDYVIPFEISSVLFLSA
                     MVGAVVIAKKD"
     gene            11935..12270
                     /gene="nuoK"
                     /locus_tag="PLU36_01365"
     CDS             11935..12270
                     /gene="nuoK"
                     /locus_tag="PLU36_01365"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HMZ45428.1"
                     /translation="MPVQYYIYLALALFSIGIIGVLTRRNTIIVFMCIELMLNAVNLL
                     LVAFSKMHHHAAYLQNKASVVGSEGQLFVFFIMVVAAAEVSVGLAIIVMLYRNTHSVD
                     INFLNRLKN"
     gene            12379..14292
                     /gene="nuoL"
                     /locus_tag="PLU36_01370"
     CDS             12379..14292
                     /gene="nuoL"
                     /locus_tag="PLU36_01370"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HMZ45429.1"
                     /translation="MSKLIYLVPLLPFIGFLINGLCRKYLTKPIIATIGSGSIFVSFI
                     ISVVLFFDVKATGLKIFKAFNFINVGAFKIDFAFQLDQLSALFLLIITGIGFLIHLYS
                     SAYMKDEEPPHYARYFAYLNLFVFSMLLLVLGANFLIMFIGWEGVGLCSYLLIGFWFK
                     NDKYNYAAKKAFVMNRIGDVGFLLAIFWLLAKVGTLSYAEVFSPSILSQLSQTDITVI
                     TILLFIGAMGKSAQIPLYTWLPDAMAGPTPVSALIHAATMVTAGIYMIARSNVLYSLS
                     EVTQHLVAIIGLATALFSATIAIKQNDIKKVLAYSTVSQLGYMFLGLGVGAYTGAVFH
                     VMTHAFFKALLFLGAGSVIHAMHHEQDIRNMGGLRKKLPITHITFLLACIAISGIPPF
                     SGFFSKDEILAAAYAHSPIYWGIGVATAALTAFYMFRLYATTFLGKFRGTQEQEHHLH
                     ESPSAMTIPLIILAVFSVVAGFVGVPAVLGGNHQLHHYLSTVLVSEKTHTIAHSTEYM
                     LMGISVAIAVIAIVFAISKYSKNPELKEPVGLGKTLANKWYVDELYDSIIVKPLLSLA
                     SFFKNIIEKLFIDGIVNNVGTFVQYASRQFRLVQSGQVANYIMIMVLAIVVFILLWFF
                     DSSILKLAGKLFY"
     gene            14309..15736
                     /locus_tag="PLU36_01375"
     CDS             14309..15736
                     /locus_tag="PLU36_01375"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HMZ45430.1"
                     /translation="MISVLLILIPLVAGLISFFIKSNNAIKQWSLFASLAVLGVVLWG
                     FTCNNNCLNIDTAWLATIGSRFTTKLDGMALSLCLLSAIVYPLIFVSTYKNEYHHANN
                     FYGLMLLALAGLMGVFVAADALLFYFFWELTLIPMYFLCSRWGGEKRIAVTFKFFVYT
                     FLGSLLMLIGILYLYYKTPNHSFALTDFYALHLSVKEQGFIFWLFFIAFAIKMPIFPF
                     HTWQPDTYEQSTTAVTMVLSAVLVKMGVFAIIRWMLPVFPLAVSSNATIVIVLSVIGM
                     LYASLLAIKQDNIKKMIAYSSIVHIGLMCAAIFANNEIALKGVILQMFNHGINILGLW
                     IVADAIEQKTGTIQFSQLGGLAQKQPVLAILFMIMALANVALPLTNAFIGEFLMFSGL
                     FQYNMYASGIALLSIILAAVYTLNMMQKVFYGNTLQATENTTEISTNVQFALIVLAIG
                     IIVLGVYPQPLIDLSKDTVMFILGK"
     gene            15761..17152
                     /locus_tag="PLU36_01380"
     CDS             15761..17152
                     /locus_tag="PLU36_01380"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HMZ45431.1"
                     /translation="MNAIIISALMGVLLMFASMATSNKQIIKNVAIVALLFLLCSNIA
                     DTYSWCTIKVNTRDMFVFTKFGLYFNSLAIAATLIYVLLSGNEIIKTGNYAAEYFTLI
                     FFVLCGISILSSFNNLLMMFLGIEIMSIPLYILTGSAKKNLKSNEAALKYFLMGSFST
                     GILLMGIALVYGASGTFDIHQVAPIGANGLQHAPLLEVIGLLLIFSAMLFKVSAAPFH
                     FWAPDVYDGSPSVFSSFMMTIVKAAGFIALINLFYIKADSLAHLWKILLFIVIIVTLV
                     VGNITAIFQQSVKRMMAYSSIAQAGFMLFALYAINDVSKEGILLYAVAYTLATVGIFA
                     ILVKIEDYTLEGYNGLAKTQPMLAFVNAIFVFSLAGIPLTAGFFAKYYMLAAVIKTGG
                     NAGIVLVIIGVIFAAVSVYYYFKILQAMYFNEGDAKVNNVSAGFKYGLFLLALTIIFI
                     GIFPDTLINWFYF"
     gene            complement(17167..17493)
                     /locus_tag="PLU36_01385"
     CDS             complement(17167..17493)
                     /locus_tag="PLU36_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HMZ45432.1"
                     /translation="MNVEQLYEYVNSKEGVEECFPFGEETLVFKVKGKMFLLVALDES
                     PLRFNAKCNPELALELREQFPQNVLPGYHMNKKHWNTIIVDGIIPNNQIKEFINHSYN
                     LVSSKK"
     gene            17644..18126
                     /locus_tag="PLU36_01390"
     CDS             17644..18126
                     /locus_tag="PLU36_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45433.1"
                     /translation="MKFHQSILLLIAVSFLACNTATTQTSSIENSDSNKDNQQIKELK
                     ENRHYRGAITGGMKGDSIFFDVTNNGTRLENLTFKGYWRCNGKLEMLRAAGPDGFFTL
                     VNNKVDDHISEPPNGGSTAWRFNLSATINGNTASGTFSMNINNLGCNTGKLQWTAVAL
                     "
     gene            18324..>19532
                     /locus_tag="PLU36_01395"
     CDS             18324..>19532
                     /locus_tag="PLU36_01395"
                     /inference="COORDINATES: protein motif:HMM:NF024956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HMZ45434.1"
                     /translation="MKHYSSKPIFKRIPALRLLIPLVAGILVQFHFKIGLNFFLYVAV
                     GSLLLFGIFFFFKPTIKYSVRWLTGISITLLIIALGGCISYIRNIENNNLWVGKFYKN
                     NVPVLLTLQEPIVEKPKSYKALAQINALYINNNWQLTKGNALIYFRKDSSKPSLQYGS
                     QIIVIKNLLPIINAGNPGGFNYAQYCAFQKIHFQVFLNDKEYAVLPNLNTNYFDNLIL
                     KTKLKVLKILRKNITEPNQLSIAEALLIGHRDDLDRDLVQSYSNTGVVHIIAISGLHL
                     GMIYGLLILLFKPFRRFKSANFLKPIFILLVLWGFCFVAGAAPSILRSAVMFSFIVVG
                     EAMSKKTTIYNSLAASAFLLLLINPFNLWDVGFQLSYCAVLSIVAFQKKIRNLFYIKN
                     KLIRFLWNLNA"
BASE COUNT         6807 a         2596 c         3572 g         6557 t
ORIGIN      
        1 aaatcttatc aaataaaagc atatcatttt tattaaactg cctgcattta tgagcaccaa
       61 tgtgaatacc attttctagt gccacttttt gagataaaat atgaggcggt ttccctacat
      121 gataatcagc tgttcctgca ctatctactt gccaattaag gtttaattta tttgctttag
      181 ttcttaatat accttctgct aaagggcttc tacaaatatt tcctaaacaa accattaaaa
      241 ttttcatcag ggcaaatata gtttttgtta ttattcttta actaatcaag catccttagt
      301 aatagtattt atatttttaa ttgcctcttt ttaaggtaat aaatagttga aacagaggag
      361 tatttaatac atagtagtat ttttttattt cataaattat tatttttata tctttccagc
      421 ccttaatact ttattagtaa tgaatagcaa cgaggataag agaagaaaaa gttatttgct
      481 aatgcgtatg acctacgata ttggcatggc tgttttttta attggcatgg ctgttttttt
      541 gttattgggt aatttttggg gaattgaaca aatcttaact attgattcta cttttagata
      601 tatgtttggc tccatttgtt tactgtatgg gttattcaga ctttatagag gtattaaaag
      661 agagtattaa tatgaaaaag tatttaataa tagctttatg ttgtttgttt gttattatgc
      721 aggcgtgtaa taatgcaaca acatataatt taaataatga tacaccacaa acaggtaaaa
      781 tatttattag tgtagatgaa agttttaagc ctgttattga acaacaaata aaagtttttc
      841 aatcatcgta tcctaaagca aatattattg cagagtataa gccagaagtt gagtgcttta
      901 aagatttgca ggaagatagt actagaatga tattagtggc tagaaatatt aatgataaag
      961 aaacggaata ttttaatgct aaactatcct acacacctaa acaagatata cttgcttacg
     1021 atgctgtggt agtaattgta aatataaata gtaaagacag catatttaca ttgcaacaac
     1081 tccaagatat tttaaatgga aaaaataata ttactgcagt aatggatggc agtaatgcta
     1141 ctagcactgt aaggttttta aaagatagta ttttaaaggg agaacaattt ggtaaaaatg
     1201 tagtggcaag caatggtagt aatgcagtgg tagaagcaat tgctaaaatg cccaatgctg
     1261 ttggctttgt tgggctaagt tgggttggca atacagatga gcaaaatcaa aaagaacagt
     1321 taaataaaat taaacttgca ttaatagaat gtgtaaaatg caatgataaa ggagtatttg
     1381 ctaaaccaag ccaaagcaca attacttatg gtcaataccc tttggcacga cccttgtact
     1441 atgtattaaa agaatattca gaagggcttg gaacagggtt taaaaatttt atgaccaacg
     1501 aaagaggcca gctaattttt agaagagctt atttagcccc tgcaaaaatg aatttaaata
     1561 caagaaatag tgcaataaaa gaagaacaat aactttataa aatttaaaac taaacgttaa
     1621 ccatttaatt aaacctgaaa atgatgaaga aaattgctac aattattttt acacttatgt
     1681 tggtttggag tggaataaat gctcaaagta tagaggatgg gaaaaaactt ttatcttatc
     1741 aaaaaaacaa aagtgctgta gatttatttc aacagttata taataaaaat acaaatagtg
     1801 cagagcatat ctattggtat ggtcaatctt taattgcaaa taaaaattat gccgaggcaa
     1861 aagcacttta tcaaaaagca ttacaagcag gtgttaacga acctttaatt tggattggtg
     1921 caggacatat tgaacttatt gaaaaaggcg atttgaattc tgccaaacaa aagtttgaac
     1981 aagctatcac tgcatctaca cctactaaag gaagaaataa acataaacca gacccttcta
     2041 tattaaacgc tataggtaga gctaatgctt atggcgattc taaaatggga gacccaaatt
     2101 atggaattga aaaactacgt caagcttctg aaattgattt aaaagatgca gaaatattag
     2161 taaatatggg caaatgctac caaaaacttg gtggagaaat gggaggcgat gcagttaaag
     2221 cttatacaga agctacagtt agagacccta aatatgctga ggcaaattgg agaatgggtt
     2281 taatttatta tagccaagat aataaagata tgtatgaaaa atactacaat gctgccatag
     2341 cttcagacat agaatttcca ccagtgtatt taagctatta cagagtgtat gaacaaacaa
     2401 atgtaaatgt ggctaaagaa tatttagata aatatatgca gtatgcagat aaagactgtg
     2461 gtaccgatta tttttatgca gattacttgt ttagagcagg taaatatcaa gaaagtttaa
     2521 ataaggcaat agaaatgagt aattctaatt gcaaaaactt tgaaagatta cctatattat
     2581 ttgcatataa ttatgataga ttaggcgaca gcattaatgc taagaaattt attgaccaat
     2641 tttttgcaac aacaaacaca gccgatatag aagcaaccga ttatgaatta gccgtaaaag
     2701 tatttagtaa attcgaggga gtagagaacc aaactgttgc ctttttacaa aaagcaatag
     2761 ataatgactc taaaaaagaa aataaactaa aatatacaaa aatggcagcc gatatttatg
     2821 gaaaagctaa aatgtataaa gaacatgtac aatggctgca taaatacaat gacctaaaag
     2881 gaagcatggg agagtatgat tattatgtta taaccaattc atcttttcaa tctaaagact
     2941 atgtgggtac aatggtttta gcacaaaaat atatagcagc atttcctaac aaaccacaag
     3001 gctatgctta taatgttgct gctgcaaaag ccttagatac atcttcaaca atttgctttg
     3061 ccgaacctgc aattttgcaa tacaatgatt ttttattgaa agattctacc actaataaat
     3121 ctcaaattgt tagcaactat tattacatca tgatatatta tgcagataaa ataaaagact
     3181 actcaaaagc attagaatat tgcaataaaa ttttattaat tatacccgat gataaagaaa
     3241 tgttagatat taaaaaagta ctagaagaaa gggccgcaaa atcgcctaaa tcaacaaaat
     3301 aattgaagca ataattagta aaactttgct tttttagaaa ttaaagtgaa atagctctcc
     3361 aatacttgga gagtttttct tttttaccca actaacacct atttttgtag cttgtaagaa
     3421 acaaattcaa ttatgcatca tttcttatta aatgcatcca atgcaaatac tacatctaac
     3481 tatcttccaa ttggtattca attaattttt gctgtgggtt ttgttgcaac tatgttattt
     3541 ttatctgcaa tggttggccc aaaaagaaga accaaagaaa aactagaaac atttactagt
     3601 ggcatagaat ctcatggcga tgcaaggcaa ccagtttcgg ttaaatattt tttagtggct
     3661 attttatttg tgttgtttga tgtagaggta atcttctttt acccttatgc agttaatttt
     3721 aaaatgttgg gctgggaagg ctttttagct gttctaatgt ttgtagctct tttcttagtt
     3781 ggatttattt atataattaa gaaaaaagca ttaaagtggg aagattaaat aaacaattgt
     3841 aagcattatg taagaatatg gttagtaacc agaagaaaaa attataaatt taaaatgaga
     3901 ccagtaaaat ataatataca tccaaaagaa gcaaaattag ccgacagttt aattgttagc
     3961 gaaatgcctg atggatatac tggcgaagga ttttttgcaa caaagcttag cgatgtagtt
     4021 ggtctggcac gcaaaaattc tatttggcca atgccttttg caaccagttg ttgtggtata
     4081 gaatttatgg ctaccatggc aagccattac gatttagcac gctttggaag cgaacgtgtg
     4141 ggcttttctc cacgccagtg cgatttgtta atggttatgg gaacaatagc taaaaaaatg
     4201 gggcctgtac tacgtcaggt ttatatacaa atggctgaac cacgttgggt aatggctgtt
     4261 ggagcttgtg caagcactgg tggtattttt gatacctata gtgtattaca aggaatagac
     4321 cagataatac ctgtagatgt gtatgtgcct ggctgtgcac caagaccaga agctatttta
     4381 gatggagtga tgagaataca agatatagta caaaatgaaa gtattagaag aagaaacaca
     4441 gaccactata aggctttatt aaacagttac ggaattgaat aaaatttatt aagttgaatt
     4501 tttaaatgat ggcattaaac aacgaaatag taaaacaaaa attagttgaa aaatttggag
     4561 aacaattaac caattgggaa gaaccttaca acttcctaac ttttgttgcc cccaaagatt
     4621 taaacttaaa agtacttcag tttttatatg atgatgaaac tcttaaattt agatttttaa
     4681 ccgatttgtg tgcagttcat tatccagata gaaaaggcga agaagttgca gttgtttatc
     4741 atttgcacaa tctagaaaac aatattagag taagatttaa agtatatacc gatgcagaaa
     4801 aaacagaagt atatacagca acagcactat acgaagctgc caattggatg gaaagagaaa
     4861 catacgattt ttttggagta aactttattg gtcatcctaa tttaaaaaga atattaaatg
     4921 tagatgaaat ggattatttt ccgttaagaa aacaatatcc attagaagat caaacaagag
     4981 ttgataaaga tgatgaaatg ttcggtcgtg gaggctctat tttgtaattt catctaacta
     5041 aaaattataa tcagctttaa aaaaaattat gtcagatatt ttacaccata atataaaact
     5101 gccaaaaggt gcaatagaaa aagaaacaac tacattaaac cttggcccaa cacaccctgc
     5161 aacacatggt gtaatgcaaa atattattga gttagatgga gaacgtattg taagtgcaga
     5221 gcaaactatt ggctatatac atagagcttt tgaaaaatta gcagaacgca gacccctatt
     5281 tcaaattacc acattaacag atagattaaa ttattgttca gcccctttaa acaatatggg
     5341 ttggcacctc acttgcgaaa aattattagg cattcaaaca cctaaaaggg tagattattt
     5401 acgagtaatt attatggaat tatctcgtat taccgaccac ttaatttgca attctattgt
     5461 tggggtagat agtggtgctt ttacaggatt cttatacgta atgcaataca gagaattggt
     5521 gtatgaaatt tatgaagaaa tatgtggtgc aagattaaca acaaacatgg ggcgtattgg
     5581 cggttttgaa agaaacttta atgataccgc atggaagaaa ttagataaat ttttagttga
     5641 atttcctaaa gcttggaaag aatttggcaa tctgttttta agaaacagaa ttttcatgga
     5701 tcgtttaatt ggaactggtg caattagtgc cgaaagagct ttaaactatg gctttactgg
     5761 tccaaattta agagctgctg gtgtagatta tgatgttaga gtacatacac cttatagtag
     5821 ttaccaagat tttaatttta ctattccagt aggtacaact ggcgataatt atgatagatt
     5881 tttagtgaga gaccaagaag tttggcaatc catttcaatc attcaacaag catatcaaaa
     5941 agtacaagaa tttaaaggag ccgaagcaga agtgtaccat gcagatgcac cagaatatta
     6001 tttgcctgaa aaagaactgg tttataccag aatggaatct ttaatatggc attttaaaat
     6061 tgtaatgggc gaaacagaaa tacccaaagg ggaagtatat aatagcattg aaggagcaaa
     6121 cggagaattg ggcttttatt taattagcga tggaggcaga acaccttata gattacattt
     6181 tagacgccct tgctttattt attatcaggc gtatagcgag ttaacaaaag gtggcatgtt
     6241 gagcgatgca attatgacaa tgagtagctt aaatttaata gctggtgagc tagatgctta
     6301 ataaaatata gaaagtaaaa ttttaaaaat tgattatttc agttatttaa aattaaaaat
     6361 taaaatagaa aagtgataca gtttaacgat acacaaatgg cagaatttaa aaggttagta
     6421 aaccgctatc ctgctgataa aaaaaagagt gccttattgc ctgtactgca tttggcacaa
     6481 gatagctttg gtggttggtt aagcaccgaa actatggatt atgtagctgc ccttttagaa
     6541 attaaaccaa tagaagtata tgaagtagct actttttata gcatgtacaa tttaaaacct
     6601 gttggtaaat atatgtttga agtttgccaa actggtcctt gtatgttaag aggcagcgat
     6661 gatattattg catatataaa tgaaaaatta ggaataaaac ctggcgaaac aacggcagat
     6721 ggaatgttta cattaaaaac agtagaatgt ttaggagcct gtggttatgc ccctatgatg
     6781 cagttaggaa agcattataa agagcattta accaaacaaa aaatagatga aattattgaa
     6841 gattgtaaaa aagcagcaat aaacaataat taaaactaat ggtggctatg cttgttacag
     6901 tacaagtgtg cgacgcaaca aaagctaaat agttgcagaa aagcagagta caaaaaaata
     6961 aattttaaaa atctctttag agatacaata tgggcagtat aaaattatta ttagaaaaag
     7021 cacatgtaga aggcatcagg tattatgatg tgtacaggcg tgaaggtggt tatagaagcg
     7081 tagaaaaagc tttaaaaaca atgagccccg aaactattgt tgaagaagtg aagaaaagtg
     7141 ggttacgtgg tcgtggtggt gctggttttc ctgctggaat gaaatggagc tttattgcaa
     7201 aaccagaagg tgtgcctcgt catttagttt gtaatgcaga cgaaagtgaa ccaggtacgt
     7261 ttaaagacag atatttaatg gagtttatac cccatttgct aatagaagga ttaatagttt
     7321 cttcgtttgc attaggcagc aacgatacat atatttatat acgtggagaa tatgcttgga
     7381 ttgtagatat tttagagcaa gccattatag aagcgaaaaa taatggctgg ttaggtaaaa
     7441 atattttagg ctcaggcttt gattgtaata ttcatgtaca acgtggtgca ggagcttata
     7501 tatgtggaga ggaaactgca ttgattgaga gccttgaagg taaaagagga aatccaagaa
     7561 taaaacctcc ttttcctgca gtacaaggtt tatggatgag acctactgta gtaaataatg
     7621 tagaaacttt agctgctgtt gtaccaatca ttaatatggg tggagaagaa tatgcaaaaa
     7681 ttggtgtagg cagaagtaca ggaacaaaac taatatcggc ttgtggaaat attaataaac
     7741 caggggtgta tgaaattgat atgaccataa gcgtagaaga atttatttat agtgatgaat
     7801 attgtggagg aatagcaaat ggtaaaagac taaaagcctg tattccaggc gggtcgtcag
     7861 tgcctatttt acctgccaac ctcttactta aaacagcaaa aggcgaaaca agatttatga
     7921 attatgaaag ccttagcgat ggaggttttg cttcaggaag tatgatgggt agcggaggct
     7981 ttattgtttt agatgaagat caatgtgtag taaaaaatac ctacaccctg gcaagatttt
     8041 atcgtcatga aagttgtggg caatgctcgc cttgtagaga aggcactggc tggatggaaa
     8101 aaatattatg gaatattgaa agtggaaaag ggaaaatgag tgatatagat ttattgtggg
     8161 atgtgcaatc taaaattgaa ggaaatacaa tttgcccatt aggcgatgca gcagcttggc
     8221 cagttgcagc agccattagg cattttagag atgagtttga atggcatatt accaataaag
     8281 aggaagcaca aaaaagaaat tttggattag cacattatgc caatccaatt catgtgcctg
     8341 cttaattttt gagtagagaa atatgtaaga aaataacttt aaaattaaac agaagtatta
     8401 ttgaagaaat aggttaaaag tgagaattgt attattagat gtttagtttg aattaatatt
     8461 atgagtgaaa cattatttaa agtaaccatt gataacattg ttattgaagt accagcaggt
     8521 actacaatat taaatgctgc aagaaaaatt gggggagaag ttgctccacc tactatgtgt
     8581 tactatagca aattagaagg cagtggaggt aaatgcagaa cttgtttagt agaggtaagt
     8641 aaaggctccg aaaaagaccc acgccctatg cctaaattag tggcaagctg ccgtaccact
     8701 gtaatggatg gaatggaagt aaaaaacatt acatccgaaa gagtattaac tgcaagagca
     8761 ggtgtagttg aatttttatt aataaaccac cccttagatt gcccaatttg tgaccaagca
     8821 ggcgaatgtt atttacaaga tttaagttat gaacatggag cagataaatc tagatttgaa
     8881 tttcaaaaaa gaacctttaa aaaagtagat ttaggagata aaatacaact acacatgaca
     8941 cgttgtattt tatgctatcg ttgtgtgtta gtagccaatc aactaactaa ccaaagagaa
     9001 catggcattt tagacagagg cgaccatgca caaattgcca cttatattca gaaaaattta
     9061 caaaacgatt ttataggaaa cataattgat gtatgcccgg taggtgcatt aacagataaa
     9121 acttttagat ttaaaaaccg tgtatggttt actaaacctg tggatgctca tagagattgc
     9181 aataacccta aatgctgcgg agaagttaga ttatggatga gaggtgacga agtatataga
     9241 gtaacaggaa gaattgatga atggggtgaa gtgaaagatg caagtaatgg caaaccaggt
     9301 tggatatgca acgaatgtag atttgataaa aagaaagtaa gcgattgggt aatagaaggc
     9361 ccaactaatg taaacagaca ttccgtaatt tctcaagggc attacgtaaa cagtgtgctg
     9421 cctcaagaaa ctttaccaga agttttaaac ggaagacagc ccaaattatt aatggacatt
     9481 cataatgtga gcgaagttaa taaaccaaat atagaattga gtgccttaga caggccagct
     9541 cattcaacag atttttaata attaattgtt atatataatg acatttttaa ctattgactg
     9601 gctatttata attgaaaaac ttgttttaat aatcattatt attttggcaa gtttaggcat
     9661 tgctttatat accacttttg cagaacgtaa agtagctgca attttacaag accgtccagg
     9721 accaaaccgt gcaggaccat ttggcttatt acaacctttg gcagatggtg taaaaatgtt
     9781 tactaaagaa gaaattgtac ctacccatgc aagtaaatgg ttatttattt taggtcccgg
     9841 tttagcaatg gttgcttctt taatgacttg tgcagtaata ccttggggcg gaagtgtaga
     9901 actatttgat agaaaaattg atttacaagt tgccgatata aatattgctg tattatacat
     9961 ttttgctgtg gtaagcttag gtatttatgg aataatgatt ggaggctggg caagcaataa
    10021 taaattttct ttaatggctg tactaagagc agcctctcag gctattagtt atgagctggc
    10081 aatgggctta agcttaattg ctgttttaat gataacagga actctatcat taaacgaaat
    10141 ggtaaaacag caacaagaag ggttatggaa tattgtttac caacctttgg gctttttatt
    10201 attttttatt tgtgcacttg cggagtgtaa cagaacacct tttgatttac ctgaagcaga
    10261 aagcgaatta aattttggct accatcaaga gtactctagt atgaaattgg gtttttattt
    10321 atttgctgaa tatattaata tgataatgag cagtgcagta atggcaaccc tatattttgg
    10381 tggatatgat attccatttg taaacgatgc taatttatat gcaagtattg gcaactgggc
    10441 agccttgcta caagtaacat ctatactgat aaaaatagtg atttttattt ttgtatttat
    10501 gtggatacgt tggacaacac caagatttag atatgatcaa ttaatggatt taggttggaa
    10561 aaaaatggtg ccattggctt tatttaatat gctagttact gcttttatag tattattaaa
    10621 acacaatggt tggaagtttt aataatagaa aacgaaactt aaaacaaagt agttttaaaa
    10681 taaagatgca ttaagtacaa aaagttgaag agtgcgacgc aacaatagat gacaaaagaa
    10741 ccagaagaat gaaacataaa acaaaacagt tgagagtatt taaattgttt agttgggtac
    10801 aaaaaatata tttgcaaacc ttagcattag ccattgctaa aagctaaaat taatagaaat
    10861 gcagacgtta actaatagat caaaacaggt agatagaagt ccgatgaact ttttagaacg
    10921 gatttattta tggaatattt ttaaagggat gttcatcact tttaaacaca tgtttaaaaa
    10981 gcaacctact attcactatc ctgaacaaaa aagaacattt agtcctgttt ttagagggct
    11041 tcatgtacta aatagagacg aagaaggcag agaacgttgt acagcttgtg ggctttgtgc
    11101 agtagcttgc ccagcagaag ctattacaat ggaagctgca gaaagattac ccgaagaaga
    11161 aaatttatac agagaagaaa agtatgcagc taagtacgaa ataaatatgc tgcgttgtat
    11221 tttttgtggc ctttgcgaag aagcttgccc caaagatgca atttatttaa gcgaaacttt
    11281 tgcaccatct aattatagca gaaagaattt tatttatagt aaacaagatt tgctaatacc
    11341 aaatcctaaa acagaaactg cagatttaga aaaagcaaaa ggaaaataga aataaacacc
    11401 atgaacttaa tacaaatatt attttggttt ttaacaggct tagcattgtt tagtgcttta
    11461 atggttatat taagcaaaaa tccagtacac agtgtactat ggctaatagt tgtattcttt
    11521 gctatctcag gtcattatat tttattaaat gcacagtttt tagcaattgt aaacttaata
    11581 gtttatgcag gggccataat ggttttgttt ttatttgtta ttatgctact taacttaaat
    11641 aaagaaacag agcctcaaaa aaatgtttgg atgaagttag ctggcaccat tgctggtggc
    11701 tgttttttat tgagtgtatg taatgttatt tacaatgcac aagtaaccac attagagcta
    11761 aataaaaaaa ccgttttaat gggtgaagga aatattggat taattgaaaa tttaggaaaa
    11821 aaattattta ccgattatgt aataccattt gaaattagta gcgtactatt tttaagtgct
    11881 atggttggtg cggtagtaat tgcaaaaaaa gattaagggt taaaaaaata aattatgcct
    11941 gtacaatatt atatatactt agcattggct ttatttagca taggaataat aggagtgctt
    12001 accagaagaa acactattat tgtttttatg tgtattgaat taatgttaaa tgcagtaaac
    12061 ctattgttgg ttgcattttc aaaaatgcat catcatgctg cctatttaca aaataaagct
    12121 tctgttgttg gtagcgaagg acagttgttt gtatttttta taatggtggt tgcagctgca
    12181 gaagtaagtg taggcttggc cataattgta atgttgtata gaaatacaca ctcggttgac
    12241 attaattttt taaacaggtt aaagaattag aaaagcatca taatattttg atgcgattaa
    12301 aataaataac ttaattttta ttatagcatt agaaaaatgt tataataaaa cctcgtaaag
    12361 aggataatag gaaactgtat gagtaaactg atttatttag taccactgtt accatttata
    12421 ggatttttaa taaatgggct atgcagaaaa tatttaacta aaccaattat tgccaccatt
    12481 ggtagtggta gcatttttgt atcatttatt attagtgtag ttttattttt tgatgtaaaa
    12541 gctacagggc ttaaaatatt taaagcattt aattttatta atgtaggagc atttaaaatt
    12601 gattttgctt ttcagttaga tcagctatct gctttatttc ttttaattat tacaggtatt
    12661 ggttttttaa tacatttata ttcctctgca tacatgaagg atgaagaacc gccacattat
    12721 gcaagatatt ttgcttactt aaacctcttt gttttttcaa tgttattatt ggttttagga
    12781 gcaaactttt taattatgtt tattggttgg gagggtgtag gcttatgttc ttatttacta
    12841 attggttttt ggtttaaaaa cgataaatat aattatgctg ctaaaaaagc ttttgtaatg
    12901 aaccgcattg gtgatgtagg ttttttatta gctatatttt ggttacttgc aaaagtgggt
    12961 acattatctt atgccgaagt tttttctcct tcaatactta gccaactttc acaaacagac
    13021 attacggtta taaccatttt attatttatt ggagcaatgg gtaaaagtgc ccaaatacct
    13081 ttatatacct ggttgccaga tgctatggct gggccaactc ctgttagtgc attaatacat
    13141 gctgcaacaa tggtaactgc tggtatttat atgattgcaa gaagtaatgt gctatatagt
    13201 ttgagtgagg ttacccaaca tcttgttgca attattggtt tagccacagc attgtttagt
    13261 gcaactatag ccataaaaca aaatgatatt aaaaaagtgt tagcttactc tacagttagt
    13321 cagttaggat atatgttttt ggggttaggt gtaggtgctt acacaggtgc tgtttttcat
    13381 gtaatgacac atgcattttt taaagcctta ttgtttttag gggcaggctc tgtaatacat
    13441 gctatgcacc acgaacaaga tattagaaat atgggaggat taagaaaaaa acttcctata
    13501 acgcatatca cttttttatt agcttgtatt gctatatccg gtattccacc atttagtggg
    13561 ttcttctcta aagatgaaat tttagctgca gcttatgctc atagcccaat ttattggggt
    13621 attggtgttg ctacagcagc attaactgca ttttatatgt tccgtttata tgctaccaca
    13681 tttttgggta aatttagagg tacacaggag caggaacatc atttgcatga aagcccaagt
    13741 gccatgacca ttccattaat tattttagcc gtttttagtg ttgttgctgg ctttgttggt
    13801 gtacctgctg ttttaggtgg caaccatcaa ttacaccact atttatcaac tgttttagtt
    13861 tcagaaaaaa cacatactat agctcacagt acagaatata tgttaatggg aatttctgta
    13921 gccattgcag taattgctat agtttttgca attagcaaat acagtaaaaa tcctgaatta
    13981 aaagaaccag ttggtttagg aaaaacattg gctaataaat ggtatgtaga tgaactttac
    14041 gattcaatta ttgtaaaacc attattgtca ttagcttcct tctttaaaaa tataattgaa
    14101 aaattattta ttgatggtat agtaaataat gttggaacat ttgtacaata tgcttctaga
    14161 caatttagat tagttcaaag tgggcaggta gccaattata ttatgataat ggttttagca
    14221 attgttgttt ttatattatt atggttcttt gattcatcaa ttttaaaatt agctggtaaa
    14281 ttattttatt aagcaataga aaataattat gatttctgta ttacttatat taattccttt
    14341 agttgcaggc ttaatatctt tttttataaa gagtaataat gcaattaagc aatggtcatt
    14401 atttgcttct ttagcggtat tgggtgttgt attatggggc tttacttgca ataataattg
    14461 tttaaatatt gatactgctt ggctagcaac tataggtagt agatttacaa ccaagttaga
    14521 tggtatggct ttatcactat gtttattatc ggcaatagta tatcctttaa tttttgtttc
    14581 aacctataaa aacgagtatc atcatgccaa taatttttat ggcttaatgc tgcttgcgtt
    14641 agctggttta atgggggtat ttgttgcggc agatgcattg ttgttttact ttttttggga
    14701 acttacttta atacctatgt attttttatg tagccgttgg ggaggagaaa aaagaattgc
    14761 agttacgttt aaattttttg tatatacttt cttaggttct ttattaatgc ttataggtat
    14821 tttatattta tattacaaaa cacctaatca tagttttgca ctaaccgact tttatgctct
    14881 acatctttca gttaaagagc agggctttat tttttggttg ttttttattg catttgcaat
    14941 taaaatgccc atctttcctt ttcatacatg gcagccagat acctacgagc aatccacaac
    15001 tgctgttaca atggtattaa gtgctgtatt agttaaaatg ggtgtttttg caattattcg
    15061 ttggatgctt cctgtgtttc cattagctgt ttcgtccaat gcaacaatag taatagtgtt
    15121 atctgtaatt ggtatgctct atgcttctct attagcaata aagcaggata atattaaaaa
    15181 aatgattgcc tattcatcaa tagtgcatat tggcttaatg tgtgctgcca tttttgcaaa
    15241 taatgaaatt gcattgaagg gggtaattct acaaatgttt aatcacggaa ttaatatttt
    15301 aggactatgg attgttgcag atgctattga acaaaaaaca ggcaccattc aatttagtca
    15361 attaggaggt ttagctcaaa aacaaccagt acttgctata ttatttatga taatggcatt
    15421 agcaaatgtt gcattgcctt taaccaatgc ttttattgga gagtttttga tgtttagtgg
    15481 tttatttcaa tacaatatgt atgcatctgg tattgcactt ttaagtatta ttttggcagc
    15541 agtttatact ttaaatatga tgcaaaaagt tttttatggt aacactttgc aagctactga
    15601 aaatacaact gaaataagca ctaatgtgca gtttgcttta attgtattgg caattggtat
    15661 tattgtatta ggggtatatc ctcaaccatt gatagattta tcaaaggata cagtaatgtt
    15721 tattttagga aaataatttt ttaaagagat taggttaatt atgaatgcaa ttattatttc
    15781 ggctttaatg ggtgtgttgc taatgtttgc aagcatggca acatcaaaca aacaaattat
    15841 aaaaaatgta gcaattgttg cattactatt tttattgtgt agcaatattg ccgatacata
    15901 tagctggtgt accattaaag taaatacaag agatatgttt gtgtttacaa agtttggttt
    15961 gtattttaac tctcttgcaa tagctgcaac tttaatatat gttttactaa gtggcaatga
    16021 aataataaaa acaggcaact atgccgcaga atattttacc cttatatttt ttgtattatg
    16081 cggtatctcc atattatctt cattcaataa tttattaatg atgtttttag gtattgaaat
    16141 catgtcaata ccattgtata ttttaacagg ctcagccaaa aagaatttaa aaagcaatga
    16201 ggcagcttta aaatattttt taatgggctc tttttctact ggcattttat taatgggtat
    16261 agcattggta tatggggcaa gtggtacttt tgatattcat caggttgcac caattggtgc
    16321 caatggttta cagcatgcac ctttattaga ggttataggg ttattattaa ttttttcagc
    16381 catgctattt aaagtgtcag ctgctccttt tcatttttgg gcacccgatg tttatgatgg
    16441 ctcgccaagt gttttctcat cgtttatgat gacaattgta aaagcggcag gttttattgc
    16501 attaattaat ttattttata ttaaagcaga tagcctggca catttatgga aaattttatt
    16561 atttatagtt attattgtaa cactggtggt gggtaatatt acagcaattt ttcagcagag
    16621 tgtaaaacgt atgatggctt actcaagtat tgcacaagca gggtttatgt tatttgcttt
    16681 atatgcaata aatgatgtat ctaaagaagg tattttatta tatgctgtag cctacacttt
    16741 agcaacagtt ggtatttttg ctatattggt taaaattgaa gattatactt tagaaggata
    16801 taatggattg gctaaaacac aacccatgct tgcatttgta aatgctattt ttgttttttc
    16861 cttagcaggt attccattaa cggctggttt ttttgctaaa tattatatgt tggcagcagt
    16921 tattaaaaca ggaggtaatg caggaatcgt tctggtaatt attggagtaa tttttgctgc
    16981 tgtaagtgta tattattatt tcaaaatatt acaggcaatg tattttaatg agggagatgc
    17041 aaaagtaaac aatgtttctg cagggtttaa atatggttta tttttattag cattaaccat
    17101 catttttatt ggtatcttcc cagatacttt aattaactgg ttttattttt aacgatttta
    17161 aagtttttat ttttttgaac taaccaagtt ataagagtgg ttaataaact ctttaatttg
    17221 attgttgggt attatgccat caacaataat agtgttccag tgttttttat tcatatggta
    17281 gccaggtagt acgttttgag ggaattgttc tcttagctct aatgctaatt caggattaca
    17341 ttttgcatta aatcgtaaag gactctcgtc taatgccact aataaaaaca ttttcccctt
    17401 tactttaaaa accaaagttt cttcgccaaa tggaaaacat tcttctactc cttctttgct
    17461 attaacatat tcataaagct gctctacatt cataaaataa aagtaaataa aaaatagtta
    17521 gtgaaacctg aagaagttta accaactagc aaactaatat tatgaaagat acttgaagac
    17581 atgaatattt tttcttgatg gtgatatttt tctatacatt atctttatcg caaaaatatt
    17641 actatgaaat ttcaccaatc aattttacta ctaattgccg tatcttttct agcatgtaat
    17701 acggctacta ctcaaacgtc aagcatagaa aatagtgatt caaataaaga taaccaacag
    17761 ataaaagagt tgaaagaaaa tagacattat agaggagcca ttacaggtgg aatgaaagga
    17821 gattctattt tttttgatgt aactaataat ggtacaaggt tggagaactt aacttttaaa
    17881 ggctattggc gttgcaatgg aaaattagaa atgttaagag ctgcaggccc cgatggtttt
    17941 tttacattag taaataataa agttgatgat catatcagcg agcctccaaa tggtggttct
    18001 acagcatggc gttttaattt gtcagcaact attaatggta atacagcttc tggaactttt
    18061 agtatgaaca tcaataatct tggatgcaac acaggtaagt tacaatggac tgcagtagct
    18121 ttataaaact taaactgtat ttgggtgtat aatctctaaa agtgtaatct tgttttattt
    18181 tacaatattg caatttgtgt tacactcttt aatattctat taatactaaa taaaatatta
    18241 tttgcagata atgtattaaa ttgggttgta atttttagta gaactttcat tttctatttt
    18301 attcatccaa ataaatagtt gcaatgaagc actattccag taaaccaatt tttaaacgaa
    18361 tacctgctct acgtttatta attcctttgg ttgctggtat tttagtgcag tttcatttta
    18421 aaataggttt aaattttttt ctttatgtag cagttggcag tctattatta tttggcattt
    18481 ttttcttctt taaaccaaca attaaatata gtgtaagatg gttaacaggt attagtatta
    18541 ctttattaat aattgcctta ggtggctgta taagctatat aagaaatatt gagaacaata
    18601 atttatgggt cggaaaattt tataaaaata atgtacctgt actacttaca ttacaagagc
    18661 caattgtaga aaaaccaaaa tcgtataaag ccttagccca aattaatgct ttatatatta
    18721 ataataattg gcagcttaca aaaggcaatg cattaattta ttttagaaaa gatagttcaa
    18781 aacccagttt gcaatatggt tcacaaatta ttgtgataaa aaatttgtta ccaataataa
    18841 atgctgggaa tccaggaggc tttaattatg ctcaatactg tgcttttcag aaaattcatt
    18901 ttcaagtttt tttaaacgat aaggaatatg ctgttttacc caatttaaac actaattatt
    18961 ttgataactt aattttaaaa accaaactaa aagtattaaa aatattaaga aaaaatatta
    19021 cagaaccaaa tcaattaagc attgcagaag ccttgctaat tggacataga gatgatttag
    19081 atagagacct tgtgcaaagt tatagtaata caggagtggt acatattatt gccataagtg
    19141 ggttgcattt aggcatgatt tatgggttac taattttatt atttaaaccc tttagaaggt
    19201 ttaagagtgc aaacttttta aagccaatat ttattttact tgtattatgg ggcttctgtt
    19261 ttgttgctgg tgcagcacca tctattttaa gaagtgcagt aatgttttct tttattgtgg
    19321 ttggagaagc tatgagtaag aaaacaacta tttacaatag cttagcagcc tctgcatttc
    19381 tgttattatt aattaatcca ttcaaccttt gggatgtagg ctttcaactt tcatattgtg
    19441 cagtactgag tattgttgct tttcagaaaa aaataagaaa tctgttttac attaaaaata
    19501 aactcatcag gtttttatgg aacttaaatg ca
//
LOCUS       DAOQPK010000014        19286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_1423_length_19286_cov_4.548749, whole genome shotgun sequence.
ACCESSION   DAOQPK010000014 DAOQPK010000000
VERSION     DAOQPK010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 19286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19286
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1423_length_19286_cov_4.548749"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..7793
                     /locus_tag="PLU36_01400"
     CDS             63..7793
                     /locus_tag="PLU36_01400"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HMZ45435.1"
                     /translation="MKLLNRLTFFVLLFGSLLFNASTVSAQLGFAASNVSNFEVAPTG
                     FNEVNTLNDVNIKTEINSFNSIHYTTCVGLTSGGTVAANQQNCVAFTPNAFTNSVSAS
                     GGSGGSIVYQWQKLVSCGSFSNISGATAATYASGSVTKTTIFKRKAWRNCSGGTGNVA
                     VYSNEVTVLIVYPGTISGDESGNCNFNSSTITGTNHSADNNYCLSDGTPQNQSYQWQK
                     SIDGGNTWTDISGANAQNYSPGSITQTAKFRRATTVSGYTTYSNVVTKTVNISPITLS
                     TESFPNSFNTGFTAPINTNFNGSTGSWGAYSTTARSTVVVHSAYYQSSPYALKMVNYS
                     TNGYAATSSKATSPKIDLSAGNNVELSFKLYTYSVSSANTCYSIKLEFSNDNGATWNL
                     IESSTAQEIANAYGQGTWNTVSLTVPGNYYNSNFRYRFATEQAAGCNYNTYLYIDDIT
                     FKSYPTCLPCPSSTNAGIDSTQCNNPDFTMAATPAPQGSTGTWSVINGSANISNVNSP
                     TSVITVTGSPATLRWTVSTPGCPDVYDDVVLTNTNNVTTLSYESFTNSFNTGFTAPIN
                     TTFTGSTGTWSAYSSTSRSTIVVNNAHVQSAPYALKIVNYNTNGYAATTSRAKSPKIN
                     LTGGNNVEMSFKLYTYSVSNSNTCFTFNVEISNDNGTTWTTVLAQTSKQLENTYGQGT
                     WNTITIPIPVSYYNANFRYRYSGVQQANCCYDTYLYIDDIRLNSNTPCVPCPSSTDAG
                     SDITQCNDANFTMTATPAPQGSTGTWSVVSGTATILNANSPTTTVTTTSSPVTLRWTL
                     VTPGCPNVTDDVVLTNNAQVVTLSNESFTNSFNTGFSAPINSTFTGAIGTWGAYSSTS
                     RSTIVVNNAHIQSAPYALKIYSVNTNGYSASTARATSPTVNLTSGSNVEMSFRLYTYA
                     VNANNTGYSFNVEFSSDNGSTWTNVFTRTSQELYNVYGQSTWNTINISVPNSYYNANF
                     KYRLSGVIKANTNYDTHLYIDDITFKSYPPCLPCPTEADAGADISNCNNATFTMDATP
                     APQGSTGTWSVISGNASISNVNSPTSSVTVSGSPATLRWTITTPGCPTIYDDVVLTNN
                     DNVVTLSTESFPNSFNTGFTAPINSTFTGSTGTWGAYSSTSRSTIVVHNSNYQSAPYA
                     LKLYNISTSGYAATTARATSPKLDLTGGDSVVMNFRILPHTVSGSNTCYTLKLEFSND
                     NGSTWNVMETGTAQDIYNTLGQGTWSTVSYVVPSMYYNANFKYRFVTSQAAGCNYNTY
                     IYVDDITFKSYPPCLPCPTNDDVDAGVDQGQCNNNIFTMSASAPPQGSTGTWSINSGT
                     ATIANPNSPTTTVTVSVSPVTLRWTLTTPNCSNVYDDVVLTLNNNVSIISNESFPNTF
                     NVNFDAPINSTFTGSTGTWSANSTNTFSTVVVNNGNYQSAPYGLRLVNHNNSGGYPAS
                     TSKATSPTINLSGTSNVELSFKLYTHTVHANNTCYTFNVEFSNDNGSTWTNVYSKTSQ
                     YLYNTFGLNTWNTITIPVPNNYYNANFKYRVSGVQQSSCNFNAYLYLDDIKVQTNTPC
                     LPCPTNNDVAAGANQDQCNNANFTMSASAPPQGSTGTWSIVSGTATIANINSPTTAVT
                     VTGSPVTLKWTITTPGCITPAFDTVTLTNTVTTTANAGSDQSICGSNSFTMSANAVGG
                     GETGTWTVISGAATISNVNSPTTLVNVTTSPAVLRWTISKNGCSTYDEVTLTVTNGSS
                     LLGDFVWWDINDNGIQDAGEPGIDGVTVKLYADANSDNIPDSNVPLFTTTTNASGQYL
                     FNNLCTGKYIVSIVTPDGYEQGTTTGTSSDPNNGSEYDNNGVAIYNGTEIRTNNVNFS
                     LNNPRVDFGLKGKLNLGNLVWMDQCNYGTKDNSETGVNGVTVYLYRDANADNLPDGSA
                     IKTTTTNVNGIYNFGTLAPGKYIVGILLPTGYASTVTTINSSNPNSDTDNDNNGIRTS
                     SGILYSNFITLTFGGEPNTGIDGDGTNGNLTLDFGIAKDTDGDRIADIIDIDDDNDGI
                     TDVNESGGFNPLGDCDNDCIPNYLDNTPGNGCPEWSDCNGDGINDFYDWDRDGIINSL
                     DLDSDNDGILDVMEARPSANAFNNHSNGMITGTDTDANGLLSSADNGSGFNNPHLNGL
                     IPHDLDRDGKPNFLDLDSDGDGITDLTEALGVYSSTGVVTGTDEDGDGVRGENFGNNG
                     ANVADNINGFGAKGVTLLDSDNDGRPNAYDIDSDNDGITDNTEAQATCSFKMPLYSDC
                     DNDGVDDAYDIGGCASCTRTSGGLTPFDKDGDSTPDYLDLDTDNDSAPDIYEGHTIKT
                     VNYWIGATGDADKDGLMDYFDGYNIITATDGLYWRNVINNNMGNFGSWDTGVGSTGSI
                     SQLPKSRPGACNVGDRDWRDFYILPVTIVEFKGNITSDAIAKLNWSVTNEININHYDL
                     ERSTNGSEFSKVAQVNAINASSSSAISNYNANDNVAGLNGIIYYRLKIVENDGTIKYS
                     NVINFKLSQSTRTVVSLYPNPAVNNFTVKITSPKDETVQVRVFDMLGKVLLSQQSTIV
                     VGINNLSFNNISKLSTGTYTVQIIMSNQVFTEKLVVTK"
     gene            complement(7913..9175)
                     /locus_tag="PLU36_01405"
     CDS             complement(7913..9175)
                     /locus_tag="PLU36_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HMZ45436.1"
                     /translation="MALHFPIVSWAIGASIYEVNIRQYTTEGTIKSFQNNLLRLKEMG
                     ITILWFMPLTPISKEKRQGSLGSYYACSAYTKVNDEFGTAEDFKELVNQAHALGLKVI
                     IDWVANHTGFNHEWAITNSDWFIKDEHGNFTEKNGWIDVIDLDYTNINMRSAMINAMQ
                     YWINEFDIDGFRCDMAHLVPLSFWYEARKHCDVIKELFWLAECDEDEYLNVFDANYAW
                     QLMHISENYCKQKSTLNQIKDLLSTYNGLPTNTYKLLFTSNHDENSWNGTEYEKYGDA
                     AKAFAVLSCTFKGISLLYSGQENPNLKRLAFFEKDTIQWNKTFLLEQFYTKLLNLRKN
                     KAISQGQTNFISISNNNILVYIRKYEENIVIVLLNLSQENKVKFMLEHEWLSGNFKNL
                     FSDFEFSFNYTSNFELQAYEARVYVKIN"
     gene            complement(9242..10966)
                     /locus_tag="PLU36_01410"
     CDS             complement(9242..10966)
                     /locus_tag="PLU36_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HMZ45437.1"
                     /translation="MKSSIDWIVLIVTLATIIAYGVYKSRTSKNLEGYFLSNRNMPWY
                     LILLSIMGTQASAITFLSAPGQAFTDGMRFVQYYFGLPLAMVALCITFVPLFGKLKIF
                     TAYEFLEQRFDVKTRAFTSFLFLLSRGLSTGISIYAPSIILSSLMGWDIFITNIIMGG
                     LLIIYTVSGGAKAVAYTQQIQLIIIFAAMFFAGYTIINLLPKNIGFIDALNISGGSNK
                     MNIITSGFENGNYNWKDKYNIISGVIGGFFLALSYFGTDQSQVGRYLTAKNTTESRLG
                     LLMNGLVKVPMQFFILLIGVLLFAFYQFKPQPIFFNPVQEQKLLQSNKKDSAILLQNE
                     FDKLNTLKTETLKNYSSNKEQTIKTIHHINDSASRIKNTYINLFNTANIEGDKNDTNY
                     IFLRFVVDYLPVGLVGLIIAIIFLAAWGSIAAALNSLAACTIIDFHKRFSKKKMIDET
                     EQAIKDYRYSKIYTLLWGIFCIVIAETVVGMGSLIEAVNVLGSLFYGVILGIFLVGFY
                     IKKVKGNAVFVAAIISEIAIIGLFVLDKNGIIGLGFLWLNVVGALLVMLLSFILQYVI
                     HGLPKSSK"
     gene            complement(11104..13614)
                     /locus_tag="PLU36_01415"
     CDS             complement(11104..13614)
                     /locus_tag="PLU36_01415"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HMZ45438.1"
                     /translation="MKKILVSSLLLITTFLKAQTNSAEIFIELKKLNVLGSVLYIAAH
                     PDDENNTLLPYLAKEKLYRTAYLSLTRGDGGQNLIGDEQGIELGMIRTQELLAARRID
                     GGEQYFTNAYEFGYSKTAEETLNKWNRLQLLYEIVWNIRKFQPDIIINRFPQDGRAGH
                     GHHAASAILAQEAFIAAADITKFPEQFTLGVQPWQAKRLLWNTFNFGNNNTTNNNQLK
                     LEVGNYNPLLGLSYGEVGGIARTMHKSQGEGRPKRKGNITEYFAYVNGDSATTDIMNR
                     VNTTWSRLEGTSIIQQKINEVIAEYNFANPQSSVSKLVTIYNLLSNYPTKNNWVTLKI
                     KEIENLIIKCSGLFIEATSSEEAVVQGQKATFNFFSNNRSDSNIKLSKISLSISNNNT
                     FDSVINLQLLKNSNYSFSKECIIPLTQPVTQPYWLTKPILKNMFHPSNQAMLGEAENS
                     PLYIAKFFLNIDGTDFTLNVPIQFKMVHPVRGEIYEPLIIVPPITVNLTPNNILLHIQ
                     QNKKIVNSKNIQLQITSNIYALKTSVTISLHQANKIVYAKDTILDLKNAESYPFNIAI
                     SNLKNYTFNEVITPSVQINYQTKEQQYTQDLKNISYEHIPNLHYFFTNETKFINEKII
                     TKPLKVGYINGAGDKVAEAIEQMGYIVTQLTEKDLNIETLKQYNAIVIGIRAYNIHEY
                     LTDKNNVLNAFVKNGGNLIVQYIKSNSIGTKAIQSGPYSFTVYSRSRITEEDAKVKFL
                     LPNHTVLNYPNKISSADFENWVQERSTYQAEKFDSNFECILGMNDTGEKETNSSLLIA
                     KYGKGNYVYCSLALFRQLPAGIAGSYKLLANMLELEKH"
     gene            complement(13657..16167)
                     /locus_tag="PLU36_01420"
     CDS             complement(13657..16167)
                     /locus_tag="PLU36_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HMZ45439.1"
                     /translation="MRNVILLIGFLFYVGNASAQTVPSPEEFLGYKIGTKYTRHHQII
                     DYVKAVAIAKKDMIIAEQYGTTNEGRPLMLLYISTPENIQNLENIRKNNLRITGILKD
                     NVKPQIKNSPSIIWLSYNVHGNEPSSSEAAMLTLYELVNPANTITKEWLKNTVVIIDP
                     CLNPDGRDRYVNWFNQMVGSNEDADPQSREHSEPWPNGRSNHYNFDLNRDWAWQTQVE
                     SQQRIKKYNQWMPQIHVDFHEQYYNNPYYFAPAAEPLHEAITPWQKEFQEIIGRNHAK
                     YFDTNGWLYFTKESFDLFYPSYGDTYPIYNGSIGMTYEQAGHSSGGLAVVKSDGDTLT
                     LTDRVFHHFTSGLSTVEAASINATKTITEFEKYFADASAGKNVVYKTYILTSDNKYNI
                     EAIKDLLNKNGILYGTIDYKGLKGYCYQLNKDAEVKPLTNTIAVSTCQPKGILANILL
                     EPKSKLNDSATYDITAWSLGYAYGVNTYAIKEKKEINYAVTLPATIRNYSSNYGYLIQ
                     YNSINSVKVLAHLLKKGIKVRFAEKPFVYNGKNYERGTLIIIKTGNDINLINKEINFA
                     ANKFDINADIVTTGFMDKGIDFGSPHVKLINTKKILLVTGESVSSLAAGEVWNFFDKQ
                     LNYPITLVNATELNRINFKNYQVIILPDGYYKFSSDKNTVDKIKEFVSTGGKLIALES
                     AVNQLAQLDVGFKLKTNPDKEPEKTDYTLLKKYADREKDYLPSSIPGAIYKIDLDNTH
                     PLAFGYDSIYYTLKLDATIYEFMKEGWNVGYLKKENYVTGFAGYKVKENLKDGTVIGA
                     TEYGRGNIIFFADNPIFRMFWENGKLMLCNAVFMVGQE"
     gene            complement(16189..17121)
                     /locus_tag="PLU36_01425"
     CDS             complement(16189..17121)
                     /locus_tag="PLU36_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HMZ45440.1"
                     /translation="MIKNWMVTVLIVIVGNLFAQDGSIKSLKEETAKKDIKKNATDTA
                     KSNWKKGGLFSLNLAQGSLSNWAAGGDKFSMSVNLYLNAHAYYKKGKNSWDNNVDFYL
                     GYVNTTTLGSRKNDDRFDLTSKYGYALNPKLNLSSLFNFRTQFYKGYEYSNNNKVLTS
                     NFMAPGYILISLGLDYKPTNDLSIFFSPITSRWVVVNDKNLSALGLYGVKPGKKSVNE
                     IGAFASVNYTKNLNKIISYKGKLDMFSNYRNKPQNIDIFMTNLFSAKISKILSATWSL
                     DLIYDDDVKLFGNNKNSPGLQLKSLFGVGLMVKL"
     gene            17268..19223
                     /locus_tag="PLU36_01430"
     CDS             17268..19223
                     /locus_tag="PLU36_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HMZ45441.1"
                     /translation="MLIGLQNVTFEFGARVIVEDATWHIHPGERIGLIGYNGTGKSTL
                     LKVLTQQYTPSKGTVEKSKDTTIGYLHQDLLSFDTNESIVEVAMSAFEQVKLLEKEIE
                     NLGNQLAENSEDESLLMLYSEKLHDLEVAGGYTVHHKTEEVLQGLGFDNKDLQRPYKE
                     FSGGWRMRVLLAKMILQQPDVLLLDEPTNHLDLPSIEWLEKYLLHYKGSVVIVSHDKY
                     FLNRMVNKIVELYQQQLHIYTGNYDYYETEKAIRVEMQNKAYENQQDYIRQNERLIER
                     FRAKASKASMAQSLMKKLDKLERIETAEIERPNIKINFQVDKQPGKIIASLNNVCKNF
                     GENVIIKNGNAEIDRGDKIALIGANGKGKSTLLRIIAGTEKFDGQREWGHNVEESFYA
                     QHQLEALNLNNTILDEMKECGSQKTELELRALLGCFLFSGDETDKKIKVLSGGEKARV
                     ALAKVIVSKSNFLMLDEPTNHLDMHSVELLAEALNKYEGSLILVSHDRYFISKTANKI
                     WEIIDHEIKEFKGTYTEWVEWNERMAKQKAQSEKQKTAIENDVVIEKKKEEKKTSNTI
                     EQTALKNELKQLQKKISKLEMDINNVNSRKEAVEADLGNPTFYTNHIEFTKLESNYKK
                     IIEEKKKLDKEYEEIFEKMMDVESAIG"
BASE COUNT         6582 a         3006 c         2974 g         6724 t
ORIGIN      
        1 attcaagtac tctaagaaac agaaatgttt cattaagcaa ttgtgcttaa aaattataaa
       61 ttatgaaatt attaaacaga cttacttttt ttgttttatt gtttggctct ttattattta
      121 atgcttcaac agtatcagct cagttgggct ttgctgcttc aaacgtttct aactttgagg
      181 ttgctccaac tgggtttaat gaagttaata cactaaacga tgtaaatatt aaaactgaaa
      241 ttaattcttt taatagtatc cattatacta catgtgttgg tcttacaagt ggtggaacag
      301 ttgctgctaa ccaacaaaat tgtgtagcat ttacacctaa tgcatttacc aattctgttt
      361 ctgcaagtgg tggttctggt ggtagcattg tttaccaatg gcaaaagttg gttagttgtg
      421 gtagtttttc taatatctct ggtgctactg cagcaactta tgcatctggt tcggttacaa
      481 aaacaacaat ttttaaaaga aaagcttgga gaaactgttc aggtggtact ggtaatgtag
      541 ctgtttattc taatgaagtt actgtattaa ttgtttatcc aggaactatt tcaggtgatg
      601 agtctggcaa ttgtaatttt aattcctcta ctattacagg taccaatcat agtgctgata
      661 acaattattg tttaagcgat ggtacaccac aaaaccaaag ttatcagtgg caaaaaagta
      721 tagatggtgg aaatacatgg acagatattt ctggagccaa tgcccaaaac tactcaccag
      781 gatctatcac acaaactgca aaatttagaa gagccactac tgttagtggt tatactactt
      841 atagcaatgt agtaactaaa actgtaaata tttcacctat tacactttct acagaaagtt
      901 ttcctaatag ttttaataca ggctttactg caccaattaa tactaatttt aatggttcta
      961 caggttcttg gggtgcatat tcaacaacag ctagatcaac agttgttgtg catagtgctt
     1021 attaccaatc ttcgccttat gctttaaaga tggttaatta cagcacaaat ggttacgctg
     1081 ctacatcatc taaagctact tctccaaaaa ttgacttatc tgcaggaaat aatgttgagt
     1141 taagttttaa attatatact tattcagttt ctagtgctaa tacttgttat agtattaaac
     1201 tagaattttc aaacgataat ggagcaactt ggaatttaat agaaagtagc actgctcaag
     1261 aaattgcaaa tgcttatggt caaggtacat ggaatactgt ttctttaact gtacctggta
     1321 attattacaa ctctaatttt agatatcgtt ttgcaacaga acaagctgct ggttgtaatt
     1381 acaatactta cttgtatata gatgatatta cattcaaatc ttatccaact tgtttgccat
     1441 gcccatctag tactaatgca ggtatagatt ctacacaatg taataaccca gattttacaa
     1501 tggctgcaac gcctgcacca caaggatcta caggtacttg gagtgtgatt aatggcagtg
     1561 caaatatttc taatgttaat tctcctacat ctgtaattac tgttacaggt tcaccagcaa
     1621 cattaagatg gacagtttcc acacctggtt gcccagatgt ttatgatgat gttgttttaa
     1681 ctaatactaa taatgttact acattatctt acgaatcttt tacaaacagt tttaatacag
     1741 gctttactgc tcctataaat accacattta ctggatcaac tggtacttgg agtgcatatt
     1801 ctagcacaag cagatctaca attgttgtta ataatgcaca tgtgcagtct gcaccttatg
     1861 ccttaaaaat tgtaaactac aatacaaacg gttatgctgc aacaacttca agagcaaaat
     1921 ctccaaaaat aaatttaaca ggcggtaata atgtagaaat gagttttaag ctttatactt
     1981 actcagtttc taacagtaat acttgtttta cttttaatgt agagatttct aatgataatg
     2041 gcacaacttg gactacagtt ttagctcaaa cttctaaaca attagagaat acctatggtc
     2101 aaggaacatg gaatacaatt actattccaa ttcctgtaag ttattataat gcaaacttca
     2161 gatatagata tagtggtgtt caacaagcaa attgttgtta tgatacttat ttatatattg
     2221 atgacattag actaaattca aatacacctt gtgtaccttg cccttcaagt acagatgctg
     2281 gttcggatat tacacaatgt aatgatgcta attttaccat gactgcaaca cctgcaccac
     2341 aaggttctac tggtacttgg agtgttgtaa gtggtacagc aactatttta aatgcaaatt
     2401 caccaacaac aactgtaaca acaacctctt ctcctgtaac tttaagatgg actttagtta
     2461 ctcctggttg tcctaatgtt actgatgatg tagttttaac caacaatgct caggtcgtta
     2521 ctttatctaa cgaatctttt acaaatagtt ttaatacagg attttcagct ccaattaact
     2581 caacatttac aggtgcaatt ggtacatggg gtgcttactc ttcaacaagt agatccacca
     2641 tagttgttaa taatgcacat atacaatctg caccttatgc attaaagatt tatagtgtaa
     2701 atacaaatgg ttatagtgca agcacagcaa gggcaacatc acctacagtt aacttaacta
     2761 gtggctctaa tgtggaaatg agttttagat tatacactta tgctgtaaat gctaataata
     2821 caggctactc ttttaacgta gaattttcga gtgataatgg tagtacatgg actaatgtat
     2881 ttacaagaac ttctcaagag ttatacaatg tgtatggtca aagtacttgg aatactatca
     2941 acatttctgt tcctaatagc tactataatg caaactttaa atatcgttta agtggtgtaa
     3001 taaaagctaa tacaaattat gacacacatt tatacataga tgatattact tttaaatcat
     3061 acccaccttg tttaccttgc ccaacagaag ctgatgcagg agctgatatt agtaattgta
     3121 ataacgcaac atttacaatg gatgctacac ctgcaccaca aggatcaact ggtacatgga
     3181 gtgttataag tggtaatgca tctatttcaa atgtaaattc tcctacaagt tcagtaactg
     3241 tttcagggtc tcctgcaact ttaagatgga caattacaac accaggctgc cctactattt
     3301 atgatgatgt tgtgttaact aataatgata atgttgttac attatcaacg gaatctttcc
     3361 ctaacagttt taatactggc tttacggcac caattaattc tacatttaca ggttctacag
     3421 gtacatgggg tgcctactca agtacatcaa gatctacaat agttgttcat aattcaaatt
     3481 atcagtctgc tccttatgct ttaaagttgt ataatattag cacaagtgga tatgcagcaa
     3541 ctacagctag agcaacctct cctaaattag atttaacagg tggtgatagt gttgtaatga
     3601 attttagaat attacctcac acagtttctg gctctaacac ttgttataca ttaaaattag
     3661 aattttctaa tgataatggt agtacatgga atgtaatgga aacaggtaca gcacaagata
     3721 tttataatac cttaggtcaa ggaacttggt caacagttag ctatgtagtt ccatctatgt
     3781 actataatgc taattttaaa tatcgttttg ttacttcaca ggctgctggt tgtaattata
     3841 atacttatat ttatgtagat gatattacat ttaaatcata ccctccatgt ttaccttgtc
     3901 ctaccaacga tgatgtagat gcaggtgtag atcaagggca atgtaataat aatatcttta
     3961 caatgtctgc ttctgcacct ccacaaggtt caacaggaac ttggagcatt aatagtggca
     4021 ctgcaactat tgcaaatcca aattctccaa caacaaccgt aacagtatca gtttctcctg
     4081 taacattaag atggacatta accactccta actgttctaa tgtatatgac gatgttgttt
     4141 tgactttaaa taataatgta agtatcattt ctaacgaatc atttccaaat acatttaatg
     4201 taaactttga tgcaccaatc aactctacct ttactggttc tacaggtact tggtctgcaa
     4261 attcaacaaa tactttctct actgttgttg taaacaatgg taattaccaa tctgcccctt
     4321 atgggttaag attagtaaac cacaacaata gtggtggata tcctgcaagc acttctaaag
     4381 ctacatctcc aactattaac ttaagtggta caagcaatgt agagttaagt tttaaacttt
     4441 acacacatac agtgcatgcc aataatactt gttatacttt taatgttgaa ttttcaaatg
     4501 ataatggaag tacttggaca aatgtgtatt ctaaaacatc tcaatattta tacaatacgt
     4561 ttggattaaa tacttggaat actattacaa tacctgttcc aaacaattac tataatgcta
     4621 attttaaata tagagtaagt ggtgttcagc aaagtagttg taattttaat gcttatttat
     4681 atttagatga tattaaggtt caaactaata caccttgttt gccttgccca acaaataacg
     4741 atgtagctgc aggtgccaat caagatcagt gtaacaatgc caactttaca atgtctgctt
     4801 ctgcaccacc acaaggctca acaggtacat ggagcattgt aagtggtact gcaacaattg
     4861 ccaacattaa ttcacctaca acagctgtta ctgtaactgg ctcacctgta actttaaaat
     4921 ggacaataac tacaccaggt tgcataacac ctgcttttga tacagttact ttaactaata
     4981 cagttactac aactgcaaat gccggttctg accaatcaat ttgtggtagt aattcattta
     5041 ctatgagtgc aaatgcagtt ggtggaggcg aaacaggtac atggactgta ataagtggtg
     5101 ctgcaacaat ttctaatgta aattctccaa caactttagt aaatgttaca acttctccag
     5161 cagttttacg ttggacaatt agcaaaaatg gttgttctac ttacgatgaa gtaactttaa
     5221 ctgtaaccaa tggctcaagc ctattaggtg attttgtatg gtgggatatt aacgataatg
     5281 gaattcaaga tgctggtgaa cctggtattg atggagtaac tgttaagtta tatgcagatg
     5341 ctaattctga caatatacct gatagtaatg ttccattatt tactacaaca actaatgctt
     5401 caggccaata tttattcaat aatctttgta ctggaaaata cattgtaagt attgtaacgc
     5461 cagatggata tgaacaaggc actacaactg gtacaagctc agatcctaat aatggtagtg
     5521 agtacgataa taatggtgta gctatttata atggtacaga aataagaact aataatgtta
     5581 acttcagcct taacaatcct agagttgatt ttggattgaa aggaaaatta aacttaggta
     5641 atttagtatg gatggatcag tgtaattatg gtactaaaga taattctgaa actggcgtaa
     5701 atggtgtgac tgtttattta tatagagatg ctaatgcaga taatttacct gatggttctg
     5761 ctataaaaac aacaacaaca aatgtaaatg gcatttacaa ctttggaact ttagctcctg
     5821 gtaaatatat tgtaggtata ttactaccta ctggctatgc ttcaacagtt actactataa
     5881 atagttcaaa ccctaatagc gatactgata atgacaataa tggtataaga acatctagcg
     5941 gaattttata cagcaacttt attacattaa catttggagg agaacctaat acaggcattg
     6001 atggtgatgg tactaacgga aacttaactt tagattttgg tattgctaaa gatactgatg
     6061 gagatagaat tgctgatatt attgatatag atgatgacaa tgatggaata actgatgtaa
     6121 atgaaagtgg tggatttaat ccattaggcg attgtgataa tgattgtatt cctaactatt
     6181 tagataatac ccctggtaat ggttgccctg aatggagtga ttgtaatggt gatggtataa
     6241 atgactttta tgactgggat agagatggta tcattaactc attagactta gacagtgata
     6301 atgatggtat tttagatgta atggaagcaa gaccatcagc caatgcattt aataaccata
     6361 gtaatggtat gataacaggt actgataccg atgcaaatgg tttattgagt tctgcagata
     6421 atggctctgg gtttaataac cctcatttga atggcttaat accacacgat ttagacagag
     6481 acggtaaacc taacttttta gatttagata gcgatggaga tggtataact gatttaactg
     6541 aagcattagg tgtatattca tctacaggtg tagtaactgg caccgatgaa gatggtgatg
     6601 gtgtaagagg tgaaaacttt ggtaacaatg gagcaaatgt agcagataat attaatggct
     6661 ttggagctaa aggagttacc cttttagata gtgataatga tggaagacca aatgcttatg
     6721 atattgatag tgataatgat ggtattactg ataatacgga agcacaagca acttgttcct
     6781 tcaaaatgcc tttatactca gattgcgata atgatggtgt agatgatgct tatgatatag
     6841 gtggctgtgc atcttgtaca agaactagtg gtggattaac tcctttcgat aaagatggag
     6901 attctacacc tgactattta gatttagata ctgataatga tagtgcacca gatatttatg
     6961 aaggccacac catcaaaact gttaactatt ggattggtgc aacaggcgat gcagacaaag
     7021 atggtttaat ggattatttt gatgggtata acattattac agcaacagat ggtttatatt
     7081 ggagaaatgt aatcaataat aatatgggca actttggttc ttgggataca ggtgttggat
     7141 ctactggtag tatttctcaa ttaccaaaaa gcagaccagg agcttgtaat gtaggtgata
     7201 gagattggag agatttctat attcttcctg taactattgt tgaatttaaa ggaaatataa
     7261 caagtgatgc tattgctaaa ttaaactgga gtgttacaaa cgaaataaat attaaccatt
     7321 atgatttaga aagaagtaca aatggttctg aatttagtaa agtagcacag gtaaatgcaa
     7381 tcaatgcttc ttcttcatct gcaatcagta attacaatgc aaatgataat gttgcaggat
     7441 taaatggaat tatttattat agattaaaaa ttgtagaaaa tgatggtact attaagtata
     7501 gtaatgtaat caatttcaaa ttaagccaat ctaccagaac tgtggtttca ttatatccaa
     7561 acccagcagt taataacttt actgttaaaa taacatcgcc aaaagatgaa acagtgcagg
     7621 tacgtgtatt tgatatgtta ggaaaagtgt tattaagcca acaatctact attgttgtag
     7681 gtatcaataa tttatctttc aataatataa gcaaattatc tacaggcacc tatactgtac
     7741 aaataattat gagcaaccaa gtgtttactg aaaaattggt tgttacaaaa taattaatcc
     7801 ctatgtttat tctaaagctg cttttattaa aagcagcctt ttttattcag taatttagct
     7861 gcattaattg gcattatatt ttgggtataa attaaattaa ctaaagtgta gtttagttta
     7921 tttttacata aactcttgct tcgtaagctt gtaattcaaa attagatgta tagttaaatg
     7981 agaattcaaa atcagaaaat aagtttttaa aattgccaga aagccattca tgctctagca
     8041 taaattttac tttattttct tgagataagt taagcaaaac tattactata ttttcctcat
     8101 attttcttat ataaactaaa atgttgttgt tgcttattga aataaaattt gtttgtccct
     8161 gagatattgc tttatttttt ctcaagttta aaagctttgt ataaaattgt tctaataaaa
     8221 aagttttatt ccattgtatt gtatcttttt caaaaaatgc caatcgtttt aggttaggat
     8281 tttcttgccc actatacagt aaggaaatac ctttaaaggt acaggataga actgcaaaag
     8341 ctttagcagc atctccatac ttttcgtatt cagttccatt ccaactattc tcatcatgat
     8401 tgctggtgaa gagtaattta taagtattgg ttggtaaacc attatatgta gaaagcaaat
     8461 cttttatttg gtttaatgtg cttttttgtt tacaataatt ttcagagata tgcattaatt
     8521 gccatgcgta attagcatca aacacgttta aatattcatc ttcatcacat tcggctaacc
     8581 aaaataattc tttaattaca tcgcaatgtt ttcttgcttc ataccaaaaa cttaaaggaa
     8641 ctaaatgtgc catatcacat ctaaaaccat caatatcaaa ttcgttaatc caatactgca
     8701 ttgcatttat cattgcagaa cgcatattga tattggtata atctaaatca attacatcta
     8761 tccaaccatt tttttctgta aaatttccat gttcatcttt aataaaccaa tcagagtttg
     8821 taattgccca ttcatggtta aaccctgtat gattggcaac ccaatcaata attaccttta
     8881 agccaagagc atgagcttga tttaccagtt ctttaaaatc ttctgctgta ccaaattcat
     8941 cattaacttt tgtataggca ctacaagcat aatagctgcc taagctacct tgcctttttt
     9001 ctttactaat aggggttaag ggcataaacc ataaaatggt tatacccatt tcttttaatc
     9061 gtagtaaatt attttgaaaa cttttaatag ttccttccgt agtatattgt ctaatattta
     9121 cttcataaat acttgcacca attgcccaac ttacaatagg aaaatgtaaa gccatatcaa
     9181 taaaatattg ctttaaatat aatttaattt attagtttat gagtagaaat aaaggttgaa
     9241 tttatttgga agatttaggc aagccatgta ttacatattg taatataaaa cttagtagca
     9301 taaccaataa agcaccaacc acatttagcc ataaaaatcc taagccaatt ataccatttt
     9361 tatctaaaac aaataagcca attatagcaa tttcgctaat aatagctgca acaaaaactg
     9421 cattaccttt cactttttta atataaaagc ctactaaaaa aatacctaaa attactccat
     9481 aaaataaaga gcctaataca tttactgctt caattaaact acccatacca acaactgttt
     9541 ctgcaataac tatacaaaat ataccccaga gtaaagtata aattttagag taacgataat
     9601 ctttaattgc ctgttccgtt tcatctatca tttttttctt actaaacctt ttatgaaaat
     9661 ctattatagt acaagctgca agagagttta aggctgctgc aatactgccc catgcagcta
     9721 aaaaaatgat ggcaataatt aatccaacta aaccaacagg taaatagtca acaacaaatc
     9781 ttaagaagat gtagttagta tcatttttat ctccttcaat atttgcagta ttaaaaaggt
     9841 ttatgtaagt attttttata cgtgatgctg aatcattaat gtgatgaatt gtttttattg
     9901 tttgctcttt attggagcta taatttttta atgtttcggt tttaagtgta tttaatttat
     9961 caaattcatt ttgcagcaaa atggcactat cttttttatt gctttgtaat aatttttgtt
    10021 cttgaacagg attgaaaaaa attggttgag gcttaaattg gtagaatgca aataacaaaa
    10081 caccaataag taaaataaaa aattgcattg gtactttaac taaaccattc attaataaac
    10141 ctaatctgct ttcggttgtg ttttttgctg ttaaatatct tccaacttgg ctttgatcgg
    10201 ttccaaaata acttaatgct aaaaagaagc caccaataac tccactaata atattgtact
    10261 tatccttcca gttataattt ccattttcaa aaccagatgt tatgatattc attttattac
    10321 taccaccact aatattaaga gcatcaataa aaccaatatt ttttggcaac aaatttataa
    10381 ttgtataacc tgcaaaaaac atggctgcaa aaatgatgat aagttgaatt tgttgtgtgt
    10441 aggcaacagc tttagctcca ccactaactg tataaattat taataaacca cccataataa
    10501 tattggtaat aaaaatatcc caacccatta aggaagataa aataatggaa ggtgcataaa
    10561 tgctaatgcc tgtgcttaac cctctggata ataaaaaaag aaaggatgta aaggctcttg
    10621 tttttacatc aaatctttgt tctaaaaatt cgtaagcagt aaaaattttt aacttaccaa
    10681 ataaaggaac aaacgtaata cacaacgcca ccattgctaa tggtaaacca aaataatatt
    10741 gtacaaaccg cataccatct gtaaaagcct gtcctggtgc agataaaaaa gtaattgcac
    10801 tagcctgtgt acccattata cttaaaagaa ttaagtacca aggcatattt ctattgctta
    10861 aaaaatatcc ttctaagttt tttgatgttc tgcttttata cactccatag gcaataatag
    10921 ttgctagggt aacaattagt actatccagt ctatactgct cttcatttat ttgtaagcat
    10981 taaaatggtt ggtgatacaa taaaaaataa ttattataac taataaaact aaagtaataa
    11041 aagcatacca gtggctccac tttttaaata aaggaatttt aggtgtactc atttttaata
    11101 atattaatgc ttttctaatt ctagcatatt agccaataat ttataacttc ctgcaatgcc
    11161 agcaggcaac tgcctaaaca atgctaagct acaataaaca taatttcctt tgccatattt
    11221 tgcaattaat aagctactgt ttgtttcttt ttctcctgta tcattcattc ctaaaataca
    11281 ttcaaaattt gaatcaaact tttctgcctg ataagtactt ctttcttgta cccagttctc
    11341 aaaatctgca gaagaaattt tattaggata atttaaaact gtatggttgg gtaacaaaaa
    11401 tttcacctta gcatcttctt ctgtaattct tgaacgacta tatactgtaa aactgtaagg
    11461 accagattga atagctttag taccaattga attagacttg atatattgaa caattaagtt
    11521 gccaccattt tttacaaatg cattaagtac attattttta tctgttaaat actcatgaat
    11581 attatatgct ctaataccta ttactattgc attatattgc ttaagcgttt caatatttaa
    11641 atctttttct gttaactgtg taactatata tcccatttgt tctattgctt ctgctacttt
    11701 atctcctgca ccatttatat acccaacttt taaaggcttt gtaataattt tttcgttaat
    11761 aaactttgtt tcgtttgtaa aaaaatagtg caaattggga atatgttcgt aactaatatt
    11821 ttttaaatcc tgtgtatatt gttgctcttt tgtctgatag tttatttgaa cagatggagt
    11881 aataacttca ttgaaagtat aattttttaa attggatatg gcaatattga atggataaga
    11941 ttctgcattt tttaaatcta aaatggtatc ttttgcatac actattttat ttgcttgatg
    12001 taatgaaata gtaacagaag tttttaaagc atatatgttt gaggtaattt gtaattgaat
    12061 atttttacta tttactattt ttttattttg ttgaatatgt agtaaaatat tattaggtgt
    12121 aagattaact gttattggtg gaacaataat taatggttca taaatttcgc ctcttaccgg
    12181 atgaaccatt ttgaattgaa taggtacatt taaagtaaaa tcagtaccat caatatttaa
    12241 aaaaaatttt gcaatataaa gtgggctgtt ttctgcttct cccaacattg cttgattgct
    12301 tggatgaaac atatttttta gaataggctt agtaagccaa tagggttgag ttacaggttg
    12361 ggttaatgga ataatacatt ctttagaaaa agagtagttg ctatttttta agagctgtaa
    12421 attaattact gaatcaaaag tgttgttatt gcttattgaa agagaaattt tacttaattt
    12481 aatattagaa tcggatctgt tattactgaa aaaattaaag gtagcttttt ggccttgtac
    12541 tacagcttct tcgctacttg ttgcttcaat aaataaacca ctacacttaa taattaaatt
    12601 ttcaatttct ttgattttta aagtaaccca attatttttt gttggatagt tgcttaataa
    12661 attataaatg gttactaatt ttgaaacgga tgattgtggg ttagcaaaat tatattctgc
    12721 tattacttca tttatttttt gttgaattat gcttgttcct tccaatctgc tccaagtagt
    12781 atttacccta ttcataatat ctgtggtagc actatctcca tttacataag caaagtattc
    12841 ggtaatattt ccttttcttt ttggtctgcc ttcaccttgg cttttgtgca tagttctggc
    12901 aatgcctcct acttcgccat aacttaagcc taacagaggg ttgtagttgc caacttctaa
    12961 ctttagttgg ttattgttgg ttgtattatt gttgccaaaa ttaaaagtat tccataacaa
    13021 cctttttgct tgccatggct gtacaccaag tgtaaactgt tctggaaatt ttgttatatc
    13081 tgctgctgca ataaatgctt cttgtgctaa aattgcacta gcagcatgat gtccatgacc
    13141 tgctctgcca tcttgtggaa acctattgat gattatatct ggttgaaatt ttcttatgtt
    13201 ccaaactatt tcatataaca gttgtaatct gttccatttg tttaatgttt cctctgctgt
    13261 tttgctataa ccaaattcat aggcattagt aaaatattgt tcaccaccat caattcttct
    13321 tgcagctaat aattcttgag ttcttatcat tcctaattct ataccttgct catcgccaat
    13381 caaattttgg cctccatctc ctctggttaa gcttaaataa gctgttctgt ataatttttc
    13441 tttagctaaa tagggtagta gtgtattatt ctcatcatct ggatgtgcag ctatgtataa
    13501 aacacttcct aaaacattta atttttttag ttcaataaat atttcggcac tattggtttg
    13561 tgcttttaag aaagtagtaa ttaataataa tgaacttact aaaatttttt tcaaaacatt
    13621 atctggttta caaaatgagt aattgggtat aaaaatttat tcttgtccaa ccataaatac
    13681 agcattgcat aacattagtt tgccgttttc ccaaaacatt ctgaaaatag gattgtcggc
    13741 aaaaaatata atatttcctc taccatattc agttgcacca ataacggtac catcttttaa
    13801 attttctttt actttatagc ctgcaaagcc ggtaacataa ttttcttttt ttaaatatcc
    13861 aacattccaa ccctctttca taaactcata aatagtagca tctaatttta atgtgtaata
    13921 aatactatca tatccaaatg ctaaaggatg tgtgttgtct aaatctattt tataaatagc
    13981 accaggaata gaagatggta aatagtcttt ttctctatcg gcatattttt taagtaaagt
    14041 gtaatctgtt ttttctggct ctttatctgg attggttttt aacttaaacc ctacatctaa
    14101 ttgtgctaac tgatttactg cactttctaa tgcaattaat ttaccaccag tggatacaaa
    14161 ttctttaatc ttatcaacag tatttttatc tgagctaaat ttataatagc catctggtaa
    14221 aataataacc tgatagtttt taaaattaat tctgtttaat tcagtagcgt ttactaatgt
    14281 aattgggtag tttaattgct tgtcaaaaaa gttccatact tcgcctgcag ctaatgagga
    14341 tacgctctct cctgtaacta ataatatttt tttggtatta attaatttta catgtgggct
    14401 accaaaatct atacctttat ccataaaacc tgttgttaca atatcggcat ttatgtcaaa
    14461 tttattggca gcaaaattta tttctttgtt gattaaattt atatcgttgc ctgttttaat
    14521 aataattaaa gtacctcttt cataattttt tccattataa acaaatggtt tttctgcaaa
    14581 tcttacttta ataccttttt ttaataaatg tgctaaaacc tttaccgaat taatactgtt
    14641 gtattgtata agataaccat aattagaaga ataatttctg attgttgcag gcagcgtaac
    14701 agcataatta atttcttttt tttcttttat tgcataggta tttacaccat aagcataccc
    14761 taggctccaa gcagtaatat cataagttgc actgtcgttt aatttacttt ttggttctaa
    14821 aagaatgttg gccaaaatac cttttggttg gcatgtactt actgcaatgg tatttgttaa
    14881 aggcttaact tctgcatctt tatttaattg gtaacaatat ccttttaacc ctttatagtc
    14941 aatagtgcca tataaaattc catttttatt gagtaagtct ttaatagcct ctatattata
    15001 tttattatcc gaggttaaaa tataagtttt gtaaacaaca tttttacctg cacttgcatc
    15061 ggcaaaatat ttttcaaatt cagtaattgt tttagtagca ttaatagatg cagcctcaac
    15121 tgttgataag ccagatgtaa aatgatgaaa aactctatct gttaatgtta atgtgtcgcc
    15181 atcactttta acaactgcta aacctccgct actatgtcct gcttgttcat aggtcattcc
    15241 tatgctacca ttgtaaatag gataagtatc gccataagag ggataaaata aatcgaaact
    15301 ttctttggta aaatataacc aaccattagt atcaaaatac tttgcatgat ttcttcctat
    15361 tatttcctga aactcttttt gccaaggtgt tatagcttcg tgtaatggct ctgctgctgg
    15421 agcaaaataa taaggattat tgtaatattg ttcatgaaaa tctacatgta tttgtggcat
    15481 ccattggtta tattttttta ttctttgttg gctttctact tgtgtttgcc aagcccaatc
    15541 tctgtttaaa tcaaaattgt aatgattgct tctgccatta ggccaaggtt cactatgttc
    15601 tctactttgt gggtctgcat cttcattact acctaccatt tgattaaacc aatttacata
    15661 tctatctctt ccatctgggt ttaaacaagg gtctataatt actacagtat tttttaacca
    15721 ctctttagtt attgtatttg cagggtttac caattcatac aaagtaagca ttgcagcttc
    15781 gctgctactt ggttcgttgc catgcacatt ataactaagc caaataatac tgggcgaatt
    15841 ttttatttgg ggttttacat tatcttttaa aattcctgtt attcttaaat tattcttacg
    15901 aatattttct aggttttgaa tattttctgg agtagaaata tataacagca tcaagggcct
    15961 tccttcgttg gttgtaccat attgttcggc aattatcata tctttttttg ctatggctac
    16021 tgcttttacg taatctataa tttgatgatg cctggtgtat ttagttccta ttttgtagcc
    16081 caaaaattct tcgggagaag gaactgtttg tgcacttgca ttaccaacat aaaaaagaaa
    16141 gccaattaat aaaattacat ttctcataat atttttattt ttatctaatt ataatttaac
    16201 cattaagccc acaccaaata aagattttaa ttgcaatcca ggagagtttt tattgtttcc
    16261 aaaaagctta acatcatcat cataaattaa atctaagctc caagttgcgg ataatatttt
    16321 agaaattttg gcagaaaata aattggtcat aaatatatca atattctgtg gtttatttct
    16381 ataattacta aacatatcta acttaccctt gtaagaaatt attttattca aatttttggt
    16441 ataattaaca cttgcaaaag caccaatttc gttaacagat ttttttcctg gctttactcc
    16501 atacaatcct aatgcagata agtttttatc gtttacaaca acccatcttg aagttatagg
    16561 agaaaagaag atggataaat cattagttgg cttataatct aaacctaaac taattaaaat
    16621 atatccagga gccataaaat ttgaagtaag cactttatta ttattactat actcgtagcc
    16681 tttataaaat tgagttctaa aattaaataa tgaacttaaa tttaatttag ggttaagtgc
    16741 ataaccatat tttgaggtta aatcaaatct gtcatcattt tttctactac ccaaagtagt
    16801 agtatttaca taacccaaat aaaaatctac attattatcc caactgtttt ttcctttttt
    16861 ataatatgca tgagcattta aatataagtt taccgacatg gaaaacttat cgccaccagc
    16921 agcccaatta cttaaagatc cttgagctaa attcaagcta aataatccac cttttttcca
    16981 attgcttttt gctgtatcgg tagcattttt tttaatatct tttttggctg tttcctcttt
    17041 taaactttta atgcttccat cttgagcaaa taaattaccc actattacta ttaaaacagt
    17101 aaccatccag tttttaatca taattattaa ctttttagac tacaataata acaagttaat
    17161 tgcttactca tttaccaatt aaacaatttt tgaattatat tttatgtttt attaaaccta
    17221 taaataacag cagcccatta cctttgctgc tttctaaaaa ataaattatg ctgataggtt
    17281 tacaaaatgt aacatttgaa tttggtgcaa gagtaattgt agaagatgct acatggcaca
    17341 tacacccagg cgaaagaatt ggcttaattg ggtataatgg cacaggaaaa tctactttat
    17401 taaaagtatt aactcaacaa tacacaccaa gtaaaggcac tgttgaaaaa agtaaagaca
    17461 ccaccattgg ttatttgcat caagatttgt taagttttga taccaatgaa tctattgtag
    17521 aagtggcaat gagtgctttt gaacaagtta aattgttaga aaaagaaatt gaaaatttag
    17581 gtaaccaatt agccgaaaat agtgaagatg aaagcttact aatgctatac agcgaaaaac
    17641 tacatgattt agaagttgca ggcggttata cagtacacca taaaactgaa gaagtattac
    17701 aaggtttagg atttgataat aaagatttac aacgccctta taaagagttt agtggaggtt
    17761 ggcgtatgag ggttttatta gctaaaatga ttttacaaca acccgatgtt ttattgttag
    17821 atgaacctac caaccactta gatttaccaa gtattgaatg gcttgaaaaa tatttattgc
    17881 attataaagg aagtgtagta attgttagcc acgacaaata tttcttaaac cgaatggtaa
    17941 acaaaattgt tgaattgtat cagcaacagt tacatattta tactggcaac tacgattatt
    18001 acgaaacaga aaaagctata cgtgtagaaa tgcaaaataa agcttatgaa aatcaacaag
    18061 attatataag acaaaatgaa agattaattg aacggtttag agctaaagcc agcaaggctt
    18121 ctatggctca aagcttaatg aaaaaattag ataagttaga aagaatagaa actgctgaaa
    18181 tagaacgtcc taacataaaa attaattttc aggtagataa acaaccagga aaaataattg
    18241 cctcattaaa taatgtatgt aaaaactttg gtgaaaatgt gataataaaa aatggaaatg
    18301 cagaaattga cagaggagat aaaattgctt taattggtgc aaacggtaaa ggaaaatcta
    18361 ctttacttag aataattgca ggaacagaaa agtttgatgg acaaagagaa tggggacata
    18421 atgttgaaga aagtttttat gctcagcacc agttagaggc attaaattta aacaacacta
    18481 ttttagatga gatgaaggag tgtggaagcc aaaaaacaga attagaatta agagccttat
    18541 tagggtgttt tttatttagt ggagatgaaa cggataaaaa aataaaagta ttaagtggtg
    18601 gcgaaaaagc aagagttgct ttggctaaag ttattgtgag taaaagcaat tttttaatgt
    18661 tagatgaacc taccaaccac ttagatatgc atagtgtaga gcttttggca gaagctttaa
    18721 ataaatacga aggtagttta attttagtga gccatgatag gtattttatt tctaaaactg
    18781 caaataaaat atgggaaata attgatcatg aaattaaaga gtttaaaggc acttacaccg
    18841 aatgggtaga atggaatgaa agaatggcta aacaaaaagc acaaagtgaa aaacaaaaaa
    18901 cagctataga aaatgatgtt gttattgaaa agaaaaagga agaaaaaaaa actagtaata
    18961 caatagaaca aacagcatta aaaaacgaac taaagcaact tcaaaaaaaa attagtaagc
    19021 tggaaatgga tataaataat gtaaatagca gaaaagaagc agtagaggct gatttaggga
    19081 atccaacatt ttacaccaac catatagaat ttactaaatt agaaagcaac tataaaaaga
    19141 ttatagaaga aaaaaagaaa ctagataaag aatacgaaga aatttttgaa aaaatgatgg
    19201 atgtagagag tgctatagga tagctttatt tggtactatc ttataaactg tgtaagtctt
    19261 cataaagttg gggtacccca acttta
//
LOCUS       DAOQPK010000015        18804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_1478_length_18804_cov_4.481892, whole genome shotgun sequence.
ACCESSION   DAOQPK010000015 DAOQPK010000000
VERSION     DAOQPK010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 18804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18804
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1478_length_18804_cov_4.481892"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PLU36_01435"
     CDS             <1..239
                     /locus_tag="PLU36_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HMZ45442.1"
                     /translation="LYNYITVEELRACTTCNACVEECPVSISPLEIILDLRRSLIMED
                     SNAPQEWNGMFSNIENNFAPWKFSPDERDKWTTE"
     gene            291..1670
                     /locus_tag="PLU36_01440"
     CDS             291..1670
                     /locus_tag="PLU36_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HMZ45443.1"
                     /translation="MNKTAAEFIAKSTIKAADLEHRRKINFNIGKYNAAVPNGKKQFN
                     NLLNTNQQAKNIKWNAIEHLDKYLLEFEEKITARGVKVIWAENANQALEAIGNICKEK
                     NCKTVVKSKSMVTEEIHLNAFLEKKGIESVETDLGEYIQQLDNEPPYHIVTPAMHKSK
                     EDVAKLFAEKLGVPNGLTPEELTQVARQTLRDKYVQAEVGITGANFVLADIGGIAITE
                     NEGNARLSCSMPKTHIVIAGIEKVLPSVNDLHLFWPLLSTYGTGQKVTVYNTIVTGAK
                     QNGEVDGPEEMYVILLDNGRTNLLENTKVREALYCIRCGACLNACPVYKNIGGHTYAT
                     TYSGPIGKVITPYMQGMNEFKHLSYASSLCGNCTEVCPVKINIHELLIENRRESVLQS
                     YSSIAERIAWKAWKKASLNRKMMNKGTGKLKNWVVNKLIKGWSKHRGDLNFSEKTFNE
                     MWQAFRTDL"
     gene            1701..2984
                     /locus_tag="PLU36_01445"
     CDS             1701..2984
                     /locus_tag="PLU36_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HMZ45444.1"
                     /translation="MIIYSTKITNRLVYIVQTLFSNSITITNNQQTYLQSALVKINYS
                     SAPITNNEIWIQPNNLLFEENITPQTIEIFYWQKLPAFFKTNGNLPFDIFSAAFFLLS
                     RYEEYLPNKKDMYGRFAHENSVAFKNNFLHLPLINLWLKELQSLYPFLKQQPTCFKFI
                     PTYDIDIAYNTKHKGFIKNTGSTLKHILKLNLSAAKENILTSIGKSNDPFDVYDDLFK
                     IHQKLNLQPIYFFLLANKRKGYDKNLNPSLKVIHELIRKHTIKYKDGVGIHPSWQSNF
                     DTKLLKSEITILEKITNQKVSKSRQHYIKMSLPETYKHLINNHITNDYSMGYGSINGF
                     RASYTNSFFWFDLSTNLTSNLSISPFCFMEANSFFEQKYNAQQAAEELEQFYKIVKSV
                     DGTFITIFHNHFITEQPQWLAWREMYFNFLLTNFQ"
     gene            3061..3891
                     /locus_tag="PLU36_01450"
     CDS             3061..3891
                     /locus_tag="PLU36_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HMZ45445.1"
                     /translation="MGKKSIPALLIAILIPLIGYFTLKSAGEDATILPKRYFFDSVIT
                     EEKDGKIITDTIWHKTANIQLVNQLGDTVSLYDIKNKAIVINIFFTSCGSICPQLTKS
                     MAKLQQSFLKGGNTRIKIDTSVVQFISLSIDPERDSVTVLKHYADSYGVNPDNWWMLT
                     GNRDSIYNFIFQELKIDKLEKVPISPEFPHTGRFVLLDKDYVVRGRVEQAYSGILKDS
                     ASMSVLARDIGLLILEKDKTKKSKIFTQIIDLSWLWLVIALLVSGFIWGFNQRRKKEK
                     "
     gene            3891..4427
                     /locus_tag="PLU36_01455"
     CDS             3891..4427
                     /locus_tag="PLU36_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HMZ45446.1"
                     /translation="MKATIQKNDKKAKTVISIISIIVFLIIVSLGRIQLNINIGFNIH
                     VFATINAYINTAIALILILALIAVKNKKYYLHKRLMSIALILSIVFLLSYIAHHILAG
                     DTKFGGVGLIKYFYYFILITHIFLAAVILPLILFTTYRGLTAEFGKHKKIARITFPLW
                     LYVAITGPTIYCLIHPYY"
     gene            4435..4893
                     /locus_tag="PLU36_01460"
     CDS             4435..4893
                     /locus_tag="PLU36_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45447.1"
                     /translation="MKKKFHPIIISTITIHTVMLCTQVLLAITLFLYLRIENPKTKLE
                     TERTTQLILVSLVLLSLFFGFYIFKKKIESLRNVKVVKEKLELFKKACVIKFVCIEFT
                     CFLALLGYFLTQNLSFMILFGVLLLFFALQRPTILSITNYLQLSNNDLYE"
     gene            4941..5588
                     /gene="rpe"
                     /locus_tag="PLU36_01465"
     CDS             4941..5588
                     /gene="rpe"
                     /locus_tag="PLU36_01465"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HMZ45448.1"
                     /translation="MPIIAPSLLSANFLQLEKDCNMLNESQADWFHLDVMDGRFVPNI
                     SFGPMIVEFISKATTKVCDVHLMIEEPEKYAEQFKNAGANILTVHLEACKHLHRNIQQ
                     IKSLGMKAGVAINPHTPVSLLEDILYDIDLVCLMSVNPGFGGQKFIPQTIEKIKQLKT
                     MCVQKNLKTYIEIDGGVTLDNADSIIKAGANVLVAGNTVFKASNPINAIAELKSK"
     gene            5655..5728
                     /locus_tag="PLU36_01470"
     tRNA            5655..5728
                     /locus_tag="PLU36_01470"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5689..5691,aa:Asp,seq:gtc)
     gene            complement(5999..7405)
                     /gene="lpdA"
                     /locus_tag="PLU36_01475"
     CDS             complement(5999..7405)
                     /gene="lpdA"
                     /locus_tag="PLU36_01475"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HMZ45449.1"
                     /translation="MTYDVVVIGSGPGGYPAAIRASQLGFKVAIIEKENLGGICLNWG
                     CIPTKALLKSAQVNEYLKHSADYGINATGSADFNAVIKRSRGVADKMSKGVQFLMKKN
                     KIDVIMGYGKVKGKGQVEVTASDNTIQIIACKYIVIATGGRSRELPNLKQDGSKVIGY
                     REAMVLANQPKSMIIVGSGAIGVEFAYVYNSMGTKVTIVEFMPRIVPVEDEDISKELE
                     KQYKKQGIEIMTNSSVESVDTTGAGVKALVKKQDGSTLTLEADVLLSAVGVVANIENI
                     GLEETGIKTEKGKVIVDKYQQTSLLGVYAIGDCTPGQALAHVASKEGINAAEHMAYLE
                     KKHHHLPDPMDYNNIPGCTYCTPEISSVGYTEKAAKEAGYEIKVGKFPFVASGKAVAG
                     GATEGFVKVIFDAKYGEFLGCHMIGANVTDMIAEAVVARKLETTAHEILNAVHPHPTM
                     SEGLKEAVAAAYGEAIDI"
     gene            complement(7487..8227)
                     /gene="recO"
                     /locus_tag="PLU36_01480"
     CDS             complement(7487..8227)
                     /gene="recO"
                     /locus_tag="PLU36_01480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HMZ45450.1"
                     /translation="MQIHSTKGIVIRAVKYGDTSIIATIYTQLFGLQSYIIKGVRGSS
                     AKSTYKANCFQPAAILELEVYHHPQKQIQFIKNYSWSYVYKNIFSNVICNSVAIYIIE
                     LLQHSIKQPEPNTDLYNFIEETLIITDTETKDTISNLPIFFTLLLCNHLGFKINGCYL
                     EITPVLDFQEGTFVAETPTHNYFAVEWEAETISWLNSINNYQDLKLLQLNRINRKIIL
                     QHLLQYFTIHIHNFGSLKSYQILQEVLD"
     gene            complement(8236..8877)
                     /locus_tag="PLU36_01485"
     CDS             complement(8236..8877)
                     /locus_tag="PLU36_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ45451.1"
                     /translation="MDTPKINIAIVDDHQIVIDGIVALLTGYHSINIVATSTSGLVML
                     EILQSKKVQILLTDIMMPAISGQDLAKQVKANFPEIKILALSMSGQGHIVSKMIEDAD
                     IAGYVLKNIGKDELFHAIENIANGGVYFSQEVLNELQHFTSLKKENETINLTVREIEI
                     IKLIEKEFSNKEIANELFISERTVETHRKNIFRKTGTSGIVGLLKYAYEHKLI"
     gene            complement(8934..11087)
                     /locus_tag="PLU36_01490"
     CDS             complement(8934..11087)
                     /locus_tag="PLU36_01490"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ45452.1"
                     /translation="MKKVLLIIFLLATVYTINAQKNKPIIDSIEKALPTQSDSAKVKS
                     YSELTWQYRSINREKAIYYGNKGIALAKKINYQEGLAQVYNDLGIIYYDKELYDSSII
                     LYNAALQIRTKLNDDLGIAKLFNKIGIVYQRQGKFGEALYNQQNALSLFEKTNFLLGI
                     SYSLNNIGILQQNLGRYNEALDYHHQSIVIKQKLKDKNGLLQSYINIANIYKITNENT
                     KAENFYKKAIELSKELENNEYLANSLNNLGNLQFSLNKLEEGKQSILESYKIRTELKD
                     SKGRVSCLTNLANILMSLNKYDSAKQVLSTAEEIALKAVNTKPELILVYQTLSKLNEI
                     TGNATASLNYYKMYAAYKDSIYSNDLSNKFSELETKYKTAQKEKQIQDQQITINKNLY
                     ELSKQEVELYQNKLNIAASNIQLFNQNELILTQRLDSTNKEKQIALLTKEKKIDKLQI
                     KNNELESNRKTTILIVSIILTILTVLIAFSGYKRYKLKKEKQLQHEIFLQKQINTQNI
                     LQAEENERKRIASDLHDGIGQLMSAAKMNLSAMETTIPFVSNEQKSSYQNALNLVDES
                     CKEIRAVSHNMMPNALLKAGLDNALKAFIYQMSNSGLEINLYTEGLETRLSANTETFL
                     YRIIQECINNVIKHAKATKLDISLIKDEDGISAMVEDNGKGFEVKNINNSEGIGLKNI
                     KSRVEYLSGTVEWKSASEKGTLIAIHIPIEAKQIA"
     gene            complement(11184..11909)
                     /gene="dapB"
                     /locus_tag="PLU36_01495"
     CDS             complement(11184..11909)
                     /gene="dapB"
                     /locus_tag="PLU36_01495"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HMZ45453.1"
                     /translation="MKIALIGYGKMGKAIEEIAVAKGHEIVLKISTNNLSDFNESNLK
                     HADVAIEFTNPHSAVKNIEQCLNFGLPIVCGSTGWLNDWHGIENLCKQKNGTLLYASN
                     FSIGVNLFFELNTFLARLMSQQKNYDVMIEEIHHTQKKDAPSGTAITLAEQVLQYLTQ
                     KNKWVNYTAEQTNELQIISERKDPAPGTHKIKYTSTIDDIEIIHTAHNRKGFANGAVL
                     AAEYIFNKKGIFTMKQVLGLEIF"
     gene            complement(11948..12604)
                     /locus_tag="PLU36_01500"
     CDS             complement(11948..12604)
                     /locus_tag="PLU36_01500"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HMZ45454.1"
                     /translation="MKIYKGILLLVFCSCILIITTSNSNAANFQRLDTSKPIVENMPS
                     TTKSKAKHNPKVATRRSAILPGWGQAYNKEYWKIPIVYGALSIPTITFIYNNNWYNRT
                     KSAYQLLYKSSQPNATHLDTLNLAKIHPKLQGLSLSSLQKFRNAFRQDRDYSVLWFVV
                     VWGLNVVDATVFGHLKEFNVSDNLSMQIKPSINPINQAKGLSLVFNINKPIIRTAKVR
                     "
     gene            complement(12613..13530)
                     /locus_tag="PLU36_01505"
     CDS             complement(12613..13530)
                     /locus_tag="PLU36_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HMZ45455.1"
                     /translation="MTTPKQNKDAIGKGLRSLLNNIDQDLKNTSGSLKSTVIEQTTGI
                     VRVPVADILVNPKQPRRDFDETELSELATSIKIHDIIQPLTVSKLANGKYQLIAGERR
                     WRASKIAALKDVPVYIRTANDKELLELALLENLQRTDLNAIEIGLSYKRMMEELNYTQ
                     EQVADRMGKERSTVTNYIRLLKLPPDIQLAVRNGKLSMGHARALITIDTVDKQLFVFS
                     EIKNKQLSVRQTEDLVRKLYKGIDTAKTPAKNNLPPAYKKIEDNLASHFSTKVNLLHN
                     KKGHGSITIEYYSLQELNKILDQMNIVVS"
     gene            complement(13580..14380)
                     /locus_tag="PLU36_01510"
     CDS             complement(13580..14380)
                     /locus_tag="PLU36_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ45456.1"
                     /translation="MARIIGVANQKGGVGKTTTAINLAASFAVLEFKTLLVDADPQAN
                     STTGVGFDLHNITTSLYDCMVNNAKANEVILKSDIPNLDLLPSHIDLVGAEIEMINYP
                     NRENVMRLLLDEVKSKYDFIVIDCSPSLGLITVNSLVAADSVVVPVQCEFFALEGLGK
                     LLNTIKIVQSRLNPELQIEGILMTMYDGRLRLSNQVVSEVRRHFDEMVFSTIIHRNTR
                     LSEAPSVGKPVILYDADSKGAVNYLNLAKEVLQKNEMTKMTNEERIIE"
     gene            complement(14634..14780)
                     /locus_tag="PLU36_01515"
     CDS             complement(14634..14780)
                     /locus_tag="PLU36_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45457.1"
                     /translation="MLLISYQFLINDQYILDSVKTNNKLIQADSIIVEFNTNSPLQHK
                     AIIP"
     gene            complement(14958..15386)
                     /locus_tag="PLU36_01520"
     CDS             complement(14958..15386)
                     /locus_tag="PLU36_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45458.1"
                     /translation="MYNGLLHLHNFLRWVIIILLVINIIKAFSGNKNIKLSLWLLISS
                     HITLLIGLYQYFFGAMGFKLFGTYGAEVMKNSALRFWAVEHIMGMVIAIALITIGHIS
                     LKKQATKKASILFLVALIIILAVVPWPFREGIGRAWFPGM"
     gene            complement(15443..16528)
                     /locus_tag="PLU36_01525"
     CDS             complement(15443..16528)
                     /locus_tag="PLU36_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45459.1"
                     /translation="MKKIILLLAVVSSYASYAQHSHQSQPVVDSMHYANEKHFKNIQQ
                     LTFGGDNAEAYFSFDGKYLIFQKTNPKEGIDCDQIFIGKLPKKGEPFNYKMVSTGRGR
                     TTCAAFLKDGKHFIYASTHLGGNECPPVPDRKKYGNRYIWPLYSSFDIFMADTSGKIV
                     KQLTNQPGYDAEGTISPDGKTMVFTSTRDGDLELYLMDLKTLKVKRITNTLGYDGGAW
                     FSPDGKKIIWRASRPKTDAEVKEYKELLADNLVAPNNMEIWVANADGSNAKQITNFGQ
                     ANWAPYFMPDSKRIIFASNHEYKRGFPFNIYTINGDGSKLTKVSRDKGFDAFPMFSYD
                     GKKIVFCSNRNNGGTRDTNIFIADWEE"
     gene            16619..17398
                     /locus_tag="PLU36_01530"
     CDS             16619..17398
                     /locus_tag="PLU36_01530"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ45460.1"
                     /translation="MKLVVLFSFIFLYACNANNNNVHHKNNSPVEYLKNQITSYPDSV
                     ELRMQLIDVLDSMKLYKEALFELDVLIKKDSFNAENWYYKAQLYEKSKDTVAAIINYK
                     RAIRIYPSPDNILTLANLYAEYRNDTALILCVEIETTYTDKKYSADCMFIAGVYYART
                     GNFTKAMELFDLCINNSYSYIVAYLEKGFIYYDNKDYKKALEIFKLSAQVNATYADSY
                     YWQAKCYEALNDKQNAISNYEKALILDKNLQEAEVAIKRLK"
     gene            complement(17404..>18804)
                     /gene="carB"
                     /locus_tag="PLU36_01535"
     CDS             complement(17404..>18804)
                     /gene="carB"
                     /locus_tag="PLU36_01535"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HMZ45461.1"
                     /translation="IDAVLPTMGGQTALNLCKEVDELGIWNKFNCKLIGVDVEAINTA
                     EDRDLFRQLMLKIGISVAPSHVANSFLEGKEHAQKIGFPLVIRSSFTLGGSGGGFVHN
                     KEELDDALKNGLEASPIHEILVEKAVLGWKEYELELLRDKNDNVVIICTVENFDPMGV
                     HTGDSITVAPAMTLSDTAFQNMRNQAILMMRSLGNFAGGCNVQFALNPETEELIAVEI
                     NPRVSRSSALASKATGYPIAKIAAKLAIGYTLDELKNQITQTTSAYFEPALDYVIVKI
                     PRWNFDKFKGANDTLGLQMKSVGEVMAIGRSFTEAIQKACQSLENNAVGLGYYGKSMM
                     KSDEIVEYIKTPKWDRIFRIKDALMQGVTVKTIAQATGIDKWFLFQIQKICTIEKELD
                     KYTLEDIPTNLLKEAKTNGFSDEQIAKILSGNVTEEEVYEKRKALGITRVYKMVDTCA
                     AEFEAKTPYFYSTFEG"
BASE COUNT         6228 a         3056 c         2662 g         6858 t
ORIGIN      
        1 ttttatacaa ctacattact gtagaagaat taagagcttg caccacttgt aatgcatgtg
       61 tagaagagtg tcctgtaagt atttcacctc ttgaaataat tcttgacctt agaagaagct
      121 taataatgga agatagtaat gcaccacaag aatggaatgg aatgttcagt aatatcgaaa
      181 ataattttgc accttggaaa ttctcgccag atgaaagaga taaatggact acagagtagt
      241 taataggaat tattactaca ttcacttcaa caaaaactta cctttacagt atgaataaga
      301 ctgctgcaga atttattgca aaaagcacca ttaaggccgc cgatttagaa catagaagaa
      361 aaataaattt taacattggc aagtacaatg cagctgtacc taatggaaaa aaacaattca
      421 acaatttatt aaatactaac caacaagcta agaatattaa atggaatgct attgaacatt
      481 tagataaata tttattagaa tttgaagaaa aaataacggc aagaggagta aaagtaattt
      541 gggccgaaaa tgctaaccag gctttagaag ctattggcaa tatatgtaaa gaaaaaaact
      601 gcaaaacagt agtaaaaagt aaaagcatgg ttacagaaga aattcactta aatgcttttc
      661 ttgaaaaaaa aggaattgaa agtgttgaaa cagatttggg agaatatatt cagcagttag
      721 ataacgaacc tccataccat attgtaacac cagcaatgca caaaagcaaa gaagacgttg
      781 caaaactttt tgcagaaaaa ttaggtgtac caaatggctt aactccagaa gaacttaccc
      841 aagttgcaag acaaacatta agagataaat atgtacaagc agaagtaggt attactggtg
      901 caaactttgt acttgcagat attggtggta tagctatcac tgaaaatgaa ggtaatgcaa
      961 gattaagttg tagcatgcct aaaacacata ttgtaattgc agggatagaa aaagtattac
     1021 cctctgtaaa cgatttgcat ttgttttggc cattgttaag cacctacgga acaggccaaa
     1081 aagtaacagt ttataatacc attgttacag gtgccaaaca aaatggtgaa gtagatgggc
     1141 ctgaagagat gtatgtaata cttttagaca atggcagaac aaatttatta gaaaatacta
     1201 aagtaagaga agccttatac tgcatacgtt gtggagcttg tttaaatgca tgtcctgttt
     1261 ataaaaatat tggtgggcat acttatgcaa caacctatag cggaccaatt ggcaaagtaa
     1321 ttacgccata tatgcaaggt atgaatgaat ttaaacattt aagttacgca tcttcattat
     1381 gtggaaattg taccgaggtt tgcccagtaa aaataaatat tcatgaatta ttaattgaaa
     1441 acagaagaga atctgtttta caaagttata gcagcattgc agaacgcatt gcatggaaag
     1501 catggaagaa agctagttta aatagaaaaa tgatgaacaa aggaacagga aaattaaaaa
     1561 attgggtggt caataaatta attaaaggct ggtctaagca tagaggtgat ttaaacttta
     1621 gcgaaaaaac ttttaatgaa atgtggcagg cctttagaac agatttataa caatttaaat
     1681 tacttgcatt tttagttgga atgattatat actccaccaa aataaccaat cgtttagtgt
     1741 atattgttca aacattattt agcaacagta ttactattac caataaccaa caaacctatt
     1801 tacaaagtgc tttagttaaa ataaattata gtagtgcacc tattactaat aacgaaattt
     1861 ggatacaacc taacaatctt ttatttgaag aaaatataac cccacaaaca atagaaattt
     1921 tttattggca aaaactaccg gcatttttta aaaccaatgg taacttacct tttgatatat
     1981 tttctgcagc ttttttttta ttaagcagat acgaagaata tttaccaaac aaaaaagata
     2041 tgtatggcag gtttgctcat gaaaacagtg tggcatttaa aaataatttt ttgcatttac
     2101 ctttaatcaa tctatggtta aaagagttgc aaagcctgta cccttttctt aaacaacaac
     2161 caacttgttt taaattcatt cctacatacg atatagatat tgcttataat actaaacaca
     2221 aagggtttat taaaaataca ggtagcactt taaagcatat cttaaaatta aatttaagtg
     2281 ctgccaaaga aaatatttta actagtatag gtaaaagcaa cgacccattt gatgtgtatg
     2341 atgatttatt taagattcac cagaaactaa acctacaacc aatatatttt tttctattag
     2401 ccaataaaag aaaaggttac gataaaaatt taaatccatc tttaaaagtt attcatgaat
     2461 taataagaaa acacaccata aaatataaag atggtgttgg tattcatcca tcatggcaaa
     2521 gtaattttga tacaaaactt ttaaaatctg aaataactat tttagaaaaa attacaaatc
     2581 aaaaagtatc taaaagcaga caacattata taaaaatgag tttgccagaa acgtataaac
     2641 acttaataaa taatcatatt acaaatgatt attctatggg ctatggaagc attaatggat
     2701 ttagagcttc atacaccaat tcattctttt ggtttgatct ttcaacaaac ttaacaagca
     2761 atcttagtat atctccattt tgctttatgg aagccaactc tttttttgaa caaaaatata
     2821 atgcccaaca agctgcagaa gaactagagc aattttacaa aattgtaaaa agtgtagatg
     2881 gtacattcat tactattttt cataatcatt ttataacaga acagcctcaa tggttggcat
     2941 ggagagaaat gtattttaat tttttgctaa ccaattttca ataaaattac aacactaatt
     3001 aatgtattaa ataccccttt attcaactac atttgcagtt aattttatta ctgctacatt
     3061 atgggtaaaa aatctattcc agctttatta attgctattt taattccact tattggttat
     3121 tttactttaa aatctgcagg agaggatgca accattctgc ctaaaagata ctttttcgat
     3181 tctgttatta cagaagaaaa agatggtaaa ataattaccg atactatttg gcacaaaaca
     3241 gcaaatattc agttagtaaa ccaattgggc gatacagttt ctttatacga tataaaaaat
     3301 aaagcaattg taataaatat attttttaca agctgtggta gtatctgtcc tcagcttacc
     3361 aaaagtatgg ccaaactaca acaaagtttt ttgaaaggtg gtaatacaag aataaaaata
     3421 gacacctctg ttgtacagtt tatttcgcta tcaatagacc cagaaagaga tagtgttacg
     3481 gttctaaaac attatgccga ttcttatggt gtaaacccag ataattggtg gatgcttaca
     3541 ggcaatagag atagtatata taattttatt tttcaagaat taaaaataga taaattagag
     3601 aaagtgccta tcagtcctga gtttcctcat actggcagat ttgttctttt agataaggat
     3661 tatgttgtaa gaggcagggt agaacaagct tacagtggca tactaaaaga ctcagcatct
     3721 atgagtgttt tagcaagaga tattggtttg cttatattag aaaaagataa aacaaaaaag
     3781 agtaaaattt tcacgcaaat aatcgattta agctggcttt ggttggtaat tgcattgtta
     3841 gtatctggtt ttatctgggg ttttaatcaa agaagaaaaa aagaaaaata atgaaagcaa
     3901 ccattcaaaa aaacgacaaa aaagctaaaa cagttattag cataatttct ataattgttt
     3961 ttttaattat tgtaagttta ggaagaatac aacttaatat aaatattggc tttaatattc
     4021 atgtatttgc taccattaat gcatatataa atacagccat agcattaatt ttaattcttg
     4081 ctttaattgc agtaaaaaat aagaaatatt atttacataa acgattaatg agcatagccc
     4141 taatattatc tattgtattt ttattaagtt atattgctca tcatatttta gcaggagata
     4201 ctaagtttgg aggcgttggt ttgataaagt atttttatta ttttatatta attacccata
     4261 tttttttagc tgctgttatt ctacctctta ttttatttac aacctataga ggtttaactg
     4321 ctgaatttgg aaagcataaa aaaatagctc gtatcacttt cccactatgg ctatatgtag
     4381 ctataacagg gccaactatt tattgtctta ttcatccata ttattaaaat aataatgaag
     4441 aaaaagtttc atccaataat aataagtaca ataacaattc atactgttat gttgtgtaca
     4501 caagttttat tagcaattac actttttctt tatcttagaa ttgaaaaccc aaaaacaaag
     4561 ttagaaactg aaagaaccac ccaacttatc cttgttagtt tagtattact aagtttattt
     4621 ttcggatttt atatttttaa gaaaaaaata gaatctttaa gaaatgtaaa agtggtgaaa
     4681 gaaaaacttg aattatttaa aaaagcatgt gtaataaagt ttgtttgtat agagtttacc
     4741 tgctttttag cattgttggg ctactttctt actcaaaatt tatcgtttat gatacttttt
     4801 ggtgtattac ttttattttt tgcattacaa agaccaacca tattatctat aaccaattac
     4861 ttgcagctta gcaacaatga tttatatgag tagatgatta tttttgtatt atctatttat
     4921 taaattttta aaatttaatt atgccaatta tagccccatc tttattaagt gctaattttt
     4981 tgcagctaga aaaagattgc aatatgctaa acgaaagcca ggcagattgg tttcatttag
     5041 atgtaatgga cggcaggttt gtacctaata tttcttttgg accaatgata gttgagttta
     5101 taagtaaagc caccacaaaa gtttgtgatg tacacttaat gatagaagaa ccagaaaaat
     5161 atgcagagca atttaaaaat gctggagcca atattttaac tgtacattta gaggcttgta
     5221 aacatttaca ccgaaatatt caacaaatta aaagccttgg tatgaaagca ggtgtagcaa
     5281 taaacccaca cacgcctgta agcttattag aagatatttt atacgatatt gatttggttt
     5341 gtttaatgag tgtaaaccca ggttttgggg ggcaaaagtt tataccacaa acaatagaaa
     5401 aaattaaaca acttaaaacc atgtgtgtac aaaagaactt aaaaacatat attgaaatag
     5461 atggtggcgt tacattagac aatgcagatt ctattattaa agctggggcc aatgttttag
     5521 tagcaggaaa tactgttttt aaagcatcta accccataaa tgcaatagct gaattaaaaa
     5581 gtaaataatt tttaaaaaag atttgctaaa gaaaaaatct tgcccttata tttgcaaccc
     5641 cgaaggggga aattggatcg gtagttcagt tggttagaat gccgccctgt cacggcggag
     5701 gtcgcgggtt cgagtcccgt ccggtccgca aaaaaggttg ttattaatag caaccttttt
     5761 tgatttagtt ttatcctaaa ctgcctgttt caaaaactaa taagggtggt tcattctcca
     5821 gctattttta gtattaccga tagtattatt taagtattcc tatcttttta tttcaaattt
     5881 atgccacata tcagcctgta tttttgtttg taatggaatg aatgcaataa taaattgcta
     5941 taaaaaacaa aggctatcta aaactagata gccttttgaa aaaatatata ttatacaatt
     6001 aaatgtctat agcctcgcca taagcagctg caacagcttc ttttaatcct tcgctcattg
     6061 ttgggtgcgg atgtacagca tttaaaattt cgtgtgctgt agtttctaat tttctggcaa
     6121 caacagcttc ggcaatcata tctgttacgt ttgcacctat catgtggcag cccaaaaatt
     6181 caccatattt tgcatcaaaa ataactttta caaatccttc agttgcacca ccagcaactg
     6241 cttttccact agcaacaaat ggaaacttac caaccttaat ttcgtaacct gcctcttttg
     6301 ctgctttttc ggtatagcca acactactta tttctggagt acaataggta catcctggaa
     6361 tattgttgta atccataggg tctggtaagt gatggtgttt tttctctaaa taagccatgt
     6421 gttctgctgc atttattcct tctttagatg ccacatgtgc taaagcttga ccaggtgtac
     6481 aatctccaat tgcatataca cctaataatg atgtttgctg atacttatca actataactt
     6541 ttcctttttc tgttttaatt cctgtttctt ctaagccaat attttcgatg tttgcaacta
     6601 ccccaactgc acttaataac acatctgcct ctaaggtaag tgtactgcca tcttgctttt
     6661 taaccagggc ttttacgcca gctccggtag tatctacact ttcaacagat gagttggtca
     6721 taatttctat accttgtttt ttgtattgct tttctaattc tttagatatg tcttcatctt
     6781 ctacaggcac aatacgtggc ataaactcaa caatggtaac tttagtaccc atgctattat
     6841 atacataagc aaactctact ccaatagcac cactaccaac aataatcatg cttttgggct
     6901 gattggctaa aaccattgct tctctgtatc caataacctt actaccatct tgttttaaat
     6961 ttggtagctc tctacttctg ccacctgttg caataacaat gtatttacaa gcaattattt
     7021 gtatagtatt atccgatgcg gttacttcta cttgtccttt tcctttcact tttccataac
     7081 ccataattac atcaatctta ttcttcttca ttaaaaactg tactccctta ctcattttat
     7141 ctgcaacacc tctgcttctt ttaattacag cattaaaatc ggctgaacct gttgcattga
     7201 tgccataatc tgcactatgt tttaaatatt catttacttg agcacttttt aatagtgctt
     7261 ttgttggaat acaaccccaa tttaaacaaa tgccacctaa gttttctttt tcaataatgg
     7321 ctactttaaa acctaattga gatgctctga tagctgcagg ataaccacct ggtccgctac
     7381 caattactac tacatcgtat gtcatagctt tttaatttta aaattgtggt tgcaaaaata
     7441 cttgcaaaag ccgaaatgga aaaatgagat tattgtatgg cttggtttaa tctaatacct
     7501 cttgtaaaat ttgatagctt tttaagctgc cgaaattatg tatatgtatt gtaaagtatt
     7561 gaagtaagtg ttgtaaaata atttttctat taattctatt taattgcaaa agttttaaat
     7621 cttgataatt attgatgctg tttaaccaac tgatagtttc tgcctcccac tcaactgcaa
     7681 aatagttatg agttggcgtt tcggcaacaa aagttccttc ttgaaaatct aaaactggtg
     7741 ttatttctaa ataacaacca tttattttga atcctaaatg attacataac agaagggtaa
     7801 aaaatatagg taaattactt atagtgtctt tcgtttcagt atcggtaatt attaatgttt
     7861 cttcaataaa attatataaa tctgtattag gttcgggttg ttttatacta tgttgtaata
     7921 gttcaataat ataaatagca acactattac aaattacatt agagaaaata tttttataca
     7981 cataactcca actataattt tttataaatt gtatttgttt ttgagggtga tgatacactt
     8041 ctaactctaa aattgcagct ggctgaaaac aatttgcctt gtaagttgat ttagctgatg
     8101 agcctcttac cccttttatg atataactct gtaagccaaa aagctgtgtg taaatggttg
     8161 ctataatgct tgtatcacca tattttactg ctcttattac aatgcctttg gtagagtgaa
     8221 tttgcatgaa agaaatcaga taagtttatg ttcgtatgca tattttagta aaccaacaat
     8281 accacttgta ccggtttttc taaaaatatt ttttctatgc gtttcaacag ttctttcact
     8341 aatgaataat tcattggcaa tttctttatt ggagaactct ttttcaatta gtttaataat
     8401 ttctatttct cttacagtta aattaatcgt ctcattttct ttcttcaaac tagtaaaatg
     8461 ctgtaattca tttaatactt cttggctaaa atacacccca ccatttgcaa tattttcaat
     8521 agcatgaaat agttcatcct ttcctatatt ctttaacaca taccctgcaa tatcagcatc
     8581 ttcaatcatt ttacttacaa tgtgcccctg gccactcatg cttaatgcaa ggattttaat
     8641 ttcaggaaaa tttgctttaa cctgcttagc taaatcttga ccacttattg caggcatcat
     8701 tatatctgta agaagtattt gaactttttt agattgtaaa atttctagca tcactaaccc
     8761 agaggtagaa gttgccacaa tatttataga atgatagcct gttaacaaag caacaatacc
     8821 atcaattact atttggtggt catctacaat tgcaatattt atttttgggg tatccattag
     8881 gggtattcta caaagtaaag tagtttttat tactagaaaa atatgtttta caattaagca
     8941 atttgtttag cttctattgg aatatgaatg gctattaagg ttcctttttc tgaggcagat
     9001 ttccattcaa cagttccgct taaatactct accctcgatt tgatattttt caatcctata
     9061 ccttccgaat tgttgatatt ttttacttca aatccttttc cattgtcttc caccattgct
     9121 gaaatcccat cttcatcttt tattaaagaa atatctaact tggttgcttt agcgtgtttt
     9181 attacattgt ttatacactc ttgaattatt ctatacaaaa aagtttcggt attggcagat
     9241 aaccttgttt ctaacccttc ggtatataaa ttaatttcta acccactatt actcatttgg
     9301 taaataaaag cttttaaggc attatctaaa cctgctttta gtaatgcatt gggcatcatg
     9361 ttatggctta ctgctctaat ttctttacaa ctttcatcta ccaagtttaa agcattttgg
     9421 tagctacttt tctgctcatt gcttacaaat ggaatagtag tttccatagc acttaagttc
     9481 attttagcag cactcattaa ttgtccaata ccatcatgca aatcgcttgc tattctttta
     9541 cgttcattct cttcggcctg taaaatattt tgtgtattaa tttgtttttg caaaaatatt
     9601 tcatgttgta attgtttctc ttttttaagt ttataccttt tataacctga gaatgcaatt
     9661 agcacagtta gtattgttaa tattatagat acaataagta ttgtagtctt tctgttgctt
     9721 tctaattcat tatttttaat ttgtaactta tcaattttct tttcttttgt taaaagggct
     9781 atttgctttt ctttattggt tgaatctaac ctttgagtta gaattaattc attttgattg
     9841 aacaactgaa tattagaagc tgcaatattt aatttatttt gatacaactc tacttcttgt
     9901 tttgataact cgtacaagtt tttattgata gttatttgct ggtcttgaat ttgtttttct
     9961 ttttgtgcag ttttgtattt tgtttccagc tcactaaatt tattactcaa atcattacta
    10021 taaatagaat ctttatatgc tgcatacatt ttatagtaat ttaaggatgc agttgcattg
    10081 cctgtaattt cattaagttt agatagtgtt tgataaacca atatcaactc tggtttagta
    10141 tttactgctt taagagcaat ttcttctgcc gttgataata cttgttttgc tgaatcgtat
    10201 ttatttaaac tcattaatat attggctaaa ttagttaaac aactcaccct gcctttagag
    10261 tcttttaatt cagttcttat tttataagat tctaaaatac tttgtttacc ttcttctaat
    10321 ttatttaagc tgaattgtaa gttgcctaag ttgtttaaac tatttgctaa atactcatta
    10381 ttctctaatt ctttagaaag ctcaattgct tttttataaa aattttctgc ttttgtattt
    10441 tcgttggtaa ttttataaat attggcaatg tttatataag attgcagtag cccatttttg
    10501 tctttaagct tttgtttaat aactatagat tgatgatgat agtctaaagc ttcattgtac
    10561 cttcctaaat tttgttgtaa aatgccaata ttattgaggg aataagaaat tcctaataaa
    10621 aaatttgttt tttcaaaaag gcttagtgca ttttgttggt tataaagtgc ttctccaaat
    10681 ttaccttgcc gttgatatac aatgccaatt ttattaaaca attttgctat acctaaatca
    10741 tcgtttaatt tagttcttat ttgcaaggct gcgttgtata aaataattga tgagtcgtac
    10801 agttctttat cataataaat aatgcccaag tcgttataaa cttgtgctaa cccctcttga
    10861 taattaattt ttttggctaa agcaattcct ttgttgccgt aataaatggc tttctctctg
    10921 tttatacttc tatattgcca agtaagttca ctatatgatt ttacttttgc actatcactt
    10981 tgtgtaggca aagctttttc aatgctatca ataataggct tatttttttg agcattaata
    11041 gtgtaaactg tagctaataa aaaaataatc agtaatactt ttttcatttg catgcctttg
    11101 tgttaaatat ataatagttt cttctattta tttatcttct tagttataga gtaaaattag
    11161 acacaaattg cataacaaat ttcttaaaaa atttctaacc ctagtacctg tttcattgta
    11221 aaaattcctt ttttgttaaa aatatattct gcagctagta ctgcaccgtt tgcaaatcct
    11281 ttgcgattgt gggctgtatg aataatttct atatcatcaa tagtagaggt atattttatt
    11341 ttatgtgtgc cgggtgcagg gtcttttctt tctgaaataa tttgtaactc atttgtttgt
    11401 tctgcggtat aatttaccca tttatttttt tgagttaagt attgtaaaac ttgttctgcc
    11461 aaagttattg ctgtaccact tggagcatct tttttttgtg tatggtgtat ttcttctatc
    11521 attacatcat aatttttttg ctggctcatt aaccttgcta aaaaagtatt taactcaaaa
    11581 aataaattta cacctatact aaagttactg gcgtataata aagtgccatt cttttgtttg
    11641 cacaaatttt caataccatg ccaatcattt agccagcctg tacttccaca aacaattggc
    11701 aaaccaaaat ttaaacattg ttctatattc tttacagcac tatgtgggtt agtaaactca
    11761 atagcaacat cggcatgttt taagttgctt tcattaaaat cggacaagtt attggtagaa
    11821 atttttaata caatttcatg cccttttgca acagcaattt cctctattgc ttttcccatt
    11881 ttgccatatc ctattaaagc aattttcata cacttagttt taaaaaatga aaaaaaatcc
    11941 tacaatttta tcgtactttg gctgtacgaa ttataggttt attgatgtta aaaaccaaac
    12001 ttaacccttt tgcctgattt ataggattaa tgctaggttt aatttgcatt gaaagattat
    12061 cgctaacatt aaactctttt aaatgtccga aaacagttgc atctactaca ttcaagcccc
    12121 aaacaacaac aaaccaaagt accgagtaat ctctatcttg cctaaaagca tttctgaatt
    12181 tttgaagaga gcttaagctt agcccttgta gttttgggtg aatttttgct aaatttagag
    12241 tatctaaatg tgtagcattg ggttgtgatg atttgtataa aagttggtaa gcacttttag
    12301 tacgattata ccaattattg ttataaataa acgttattgt tggtatagaa agggcaccgt
    12361 acacaatggg tattttccag tattctttat tataagcctg cccccaacct ggtaaaatag
    12421 ctgaacgtct tgttgcaact tttggattat gctttgcttt tgatttggtt gtagaaggca
    12481 tattttctac aattggttta ctagtatcta acctttgaaa gttggctgca tttgaatttg
    12541 aagttgtaat aattaagata caactacaaa acaccaacaa caatatgcct ttataaattt
    12601 tcattaaaaa atttagctta caacaatatt catctggtct aaaattttat tcaattcctg
    12661 taatgagtaa tactctatgg taatactgcc atgccctttt ttgttgtgca ataaatttac
    12721 tttagtgcta aaatgtgaag ctaaattatc ttcaattttt ttataggctg gtggtaaatt
    12781 atttttagca ggagttttag cagtatctat tcctttatat aattttctaa ctaagtcttc
    12841 tgtttgtctt acacttaatt gtttgttttt tatttcgctg aaaacaaaaa gttgtttatc
    12901 aactgtatca atggtaatta aggcccttgc atgccccata gataatttgc catttcttac
    12961 agccaattgt atatctggcg gaagttttaa taaacgaata tagttggtta cagtgcttct
    13021 ttctttaccc attctgtcag ctacttgctc ttgcgtataa tttaactctt ccatcatacg
    13081 tttgtagctt agtccaattt caattgcatt taaatctgta cgctgcaggt tttctaacaa
    13141 agcaagctct aataattctt tatcgtttgc agtacgaatg taaacaggaa catcttttaa
    13201 tgctgcaatt ttagaagctc tccatcttct ttcgcctgct attaactgat actttccatt
    13261 ggctaattta cttacagtta atggttgtat aatatcatga atttttatag aagtagctaa
    13321 ttcacttaat tctgtttcat caaaatctct tcttggttgt tttggattaa ctaaaatatc
    13381 ggctactggt acacgtacaa tgcctgtagt ttgctcaata actgtacttt ttaatgagcc
    13441 tgaagtattt tttaaatctt gatctatatt atttaaaagg cttcttaagc ctttgccaat
    13501 agcatcttta ttttgtttgg gcgttgtcat ttaatgcaaa taaaattatt taacttgttg
    13561 aagcgttcga aagatatgct tattcaatta ttctttcttc gttggtcatt ttggtcattt
    13621 catttttttg taaaacttct ttagcaaggt ttaaatagtt tacagcacct ttactatctg
    13681 catcatacaa aataactggt ttaccaacac ttggggcttc acttaatcta gtatttctgt
    13741 gtataatagt gctaaacacc atttcatcaa aatgccttct tacctcacta accacttggt
    13801 tacttaaacg taaacgtcca tcatacatgg tcattaatat tccttcaatt tgtaattcag
    13861 gatttaatct gctttgtaca atttttatag tattcaatag ttttcctaaa ccttctaaag
    13921 caaaaaattc gcattgtaca ggtactacta cactatctgc tgctactaaa gaatttactg
    13981 taattaaacc taatgatggg gagcagtcaa ttacaataaa atcgtatttg ctttttacct
    14041 catctaatag caaacgcatt acattttctc tgttggggta attaatcatt tcaatttctg
    14101 caccaactaa atctatatga gagggtaata agtctaaatt aggaatatca ctttttaaaa
    14161 taacttcatt tgctttggca ttatttacca tacaatcgta caaactagtg gtaatattat
    14221 gaaggtcgaa accaacccct gttgtactat tagcttgtgg atcagcatca accaacaaag
    14281 ttttaaattc tagcacagca aaacttgctg ctaaattaat tgcagtggtt gttttaccaa
    14341 cgccaccttt ttgattagct acaccaataa ttctagccat aattctcgtt atcttctatt
    14401 ttaaaaactt tcaaaaatta caaatttata ataataagtt actctacaaa gaaccgaaat
    14461 ttatcaatgc tttagtgtag taattttccc cacttttttc cccttaaatt gtttaaatat
    14521 aatttgatta atttatacac agaaggaact aaatatattc atgcttttat ctctacaaca
    14581 aggtattcaa ttttatacaa agtacttttt acaaattatt ataaaaatag tggttaagga
    14641 ataattgcct tgtgttgtag agggctatta gtattaaact ctacaataat actatctgct
    14701 tgtattagtt tattgtttgt tttaacacta tctaaaatat attggtcatt tattagaaat
    14761 tgatagctaa ttaacaacat agagtcggta gttagttttc tttctataat ctttgcttta
    14821 cattgttgtt tattactgtt gcaagcaatt agtaaaatta ttatgaaact acaaaaagct
    14881 agtattttaa aattcatact gcaaataaaa caaaaaaggc ttcgtaaacg aaaccttttt
    14941 aaaaaattaa aagttaatta cataccagga aaccaagctc ttccaatacc ctctctaaaa
    15001 ggccaaggta caactgctag aataattatt aaggcaacca aaaatagaat tgatgctttt
    15061 ttagtggctt gttttttaag cgatatatgc ccaatagtaa ttaaagcaat ggcaattacc
    15121 atacccataa tatgctctac agcccaaaag cgtaaggcac tatttttcat tacttcggca
    15181 ccataagttc caaaaagttt aaaacccatt gccccaaaaa agtattgata caggccgatt
    15241 aatagtgtaa tatggctact tattaacagc cataagctaa gtttaatatt tttatttccg
    15301 ctaaaggctt taataatatt aattactaat aatattatta ttacccaacg taaaaaattg
    15361 tgtaagtgta ataatccgtt gtacataatt ggtagatttt tacaaaacta agggtatttg
    15421 ttttgtatat agatatatta gtttattctt cccaatctgc tataaaaata ttagtatctc
    15481 ttgtaccacc attgtttctg ttactacaaa aaactatttt tttaccatca taactaaaca
    15541 taggaaatgc atcaaaaccc ttatctctac taacttttgt taacttgcta ccatctccat
    15601 taatggtata aatattaaac ggaaagcctc ttttatattc atggttacta gcaaaaataa
    15661 ttcttttgct atctggcata aaataaggtg cccagttagc ttggccaaaa ttggttattt
    15721 gtttagcgtt actgccatcg gcattggcaa cccaaatttc catattatta ggtgctacca
    15781 aattatcggc taatagctct ttatattctt tcacttctgc atctgtttta gggcgactag
    15841 cacgccaaat tatttttttg ccatctgggc taaaccatgc tccaccatca taccctaatg
    15901 tattggttat tcgttttact ttaagcgttt ttaaatccat taagtacaat tctaaatctc
    15961 catctcttgt gcttgtaaaa accattgttt taccatctgg cgaaatagtt ccttctgcat
    16021 cgtatccagg ttggtttgtt aattgtttaa ctattttgcc acttgtatca gccataaata
    16081 tatcaaagct gctgtataaa ggccatatat atctattgcc atattttttt ctatctggta
    16141 caggtgggca ttcatttcca cctaaatgtg tagaggcata aataaaatgc ttaccatctt
    16201 ttaaaaatgc agcacaagtt gttctacccc tgccagtact taccatttta tagttaaatg
    16261 gttcgccttt ttttggtaat tttcctataa aaatttgatc acagtcaatg ccttctttag
    16321 ggtttgtttt ttgaaaaatt aaatattttc catcaaaact aaaataagct tcggcattat
    16381 cgccaccaaa agttagttgc tgaatatttt tgaaatgttt ttcatttgca tagtgcatag
    16441 agtcaacaac aggttgagat tgatggctat gttgtgcata gcttgcataa ctacttacaa
    16501 cagctaataa aagtataatt tttttcatgc tgcaaagtaa atgtgaaaac tattaaaagg
    16561 ctttggataa atgtcagata aatgtaaaat tattactaag gatttttatt tttgtgcaat
    16621 gaaattagtt gtattatttt ctttcatttt tctttatgca tgtaatgcaa acaataataa
    16681 tgtacatcac aaaaacaatt cacctgtaga atatttaaaa aaccaaatta caagttaccc
    16741 agatagtgtt gaattgagaa tgcagttaat tgatgtttta gatagtatga aactatataa
    16801 agaagcgtta tttgaattag atgttttaat aaaaaaagat agttttaatg cagagaattg
    16861 gtattataaa gcacaacttt atgaaaaatc taaagatact gttgccgcaa ttattaatta
    16921 taaaagagca attagaattt atccatctcc cgataatatt ttaacccttg caaatcttta
    16981 tgcagagtac agaaacgata ctgcactaat tttatgtgtt gaaatagaaa caacttatac
    17041 cgataaaaaa tattctgccg attgtatgtt tattgcaggt gtgtattatg caagaacagg
    17101 aaattttact aaagcaatgg aactttttga cctttgtatt aataatagct attcttatat
    17161 tgttgcttat ttagaaaaag gctttattta ttacgataat aaagattata aaaaggcatt
    17221 agaaattttt aaactttctg ctcaagttaa tgcaacttat gccgactcat attactggca
    17281 agctaaatgt tacgaagcat taaacgataa acaaaatgca ataagtaatt acgaaaaagc
    17341 cttaatatta gacaaaaatt tacaggaagc tgaagtagct attaaaaggt taaaataaaa
    17401 ttattaacct tcaaaagtgc tataaaaata aggcgtttta gcttcaaact ctgctgcaca
    17461 agtatctacc attttataca ctcttgtaat gcctaatgct tttctttttt catatacttc
    17521 ttcttcggta acattaccac ttaaaatttt ggctatttgc tcatcactaa atccattggt
    17581 tttagcctct tttaataaat ttgttggtat atcctctaaa gtatatttat ccagttcctt
    17641 ctctatggta caaatttttt gaatttgaaa taaaaaccac ttatcaatac ctgttgcctg
    17701 agcaattgtt ttaacagtta caccttgcat taaagcatct ttaattctaa atattctatc
    17761 ccatttggga gtttttatat actccacaat ctcatcactt ttcatcatac tcttgccata
    17821 ataacccaaa ccaactgcat tattctctaa actctgacag gccttttgaa tggcttctgt
    17881 aaaacttctg ccaattgcca ttacctcgcc aacacttttc atttgtaagc ctaaagtatc
    17941 gttggctcct ttaaatttat caaaattcca acgcggaatt tttacaatta cataatctaa
    18001 agctggttca aaataggcac ttgttgtttg cgtaatttga ttttttaatt cgtctaaagt
    18061 atatcctatg gctaatttag ctgcaatttt tgcaatagga taaccggttg ctttacttgc
    18121 caaagcacta ctacggctta cacgtggatt aatttctact gctattaact cttcagtttc
    18181 tggatttaat gcaaattgca cattacaacc cccagcaaaa ttacccaagc ttcgcatcat
    18241 taatattgct tgatttcgca tattttgaaa tgctgtatca cttaatgtca tggcaggtgc
    18301 aacagttata ctatcgcctg tatgaacacc cattgggtca aaattttcta ctgtacaaat
    18361 aataacaaca ttatcatttt tatctcttag taattctaat tcatattctt tccagcctaa
    18421 aacagccttc tctactaaaa tttcatgaat agggcttgct tctaatccat ttttcaaagc
    18481 atcatctagt tcttctttat tatgcacaaa tcctcctcca ctacctccta aagtaaatga
    18541 tgaacgtatt actaacggaa agccaatttt ttgtgcatgc tcttttcctt ctaaaaaact
    18601 atttgccaca tggcttggtg ctacactaat tcctatttta agcattaatt gtctaaacaa
    18661 atctctatct tctgcagtat taattgcttc tacatctaca ccaataagct tacaattaaa
    18721 tttattccaa attcctaatt catccacttc tttacaaagg tttaaggcag tttgcccacc
    18781 cattgttggt aacactgcat ctat
//
LOCUS       DAOQPK010000016        18493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_1505_length_18493_cov_4.193080, whole genome shotgun sequence.
ACCESSION   DAOQPK010000016 DAOQPK010000000
VERSION     DAOQPK010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 18493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18493
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1505_length_18493_cov_4.193080"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /gene="glyA"
                     /locus_tag="PLU36_01540"
     CDS             complement(<1..231)
                     /gene="glyA"
                     /locus_tag="PLU36_01540"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616685.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HMZ45462.1"
                     /translation="MQRDTQVFDLINQELQRQRRGIELIASENFTSLQVMQAMGGVMT
                     NKYAEGYPGRRYYGGCEIVDQTEQLAIDRLKQI"
     gene            567..1559
                     /locus_tag="PLU36_01545"
     CDS             567..1559
                     /locus_tag="PLU36_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HMZ45463.1"
                     /translation="MKAIIPVAGAGTKLRPLTYTQPKALIPLAGKAILGFIVDQLKNA
                     GINEFIFIVGYFGDKIQDYIKQNYPSINAHFVYQNERHGTGHAIELTRHIVDSDEVFI
                     ALGDTICEYDLNEVIHSPFNMLGVRKIDDPRNFGVATINEEGFIENVVEKPAFPKSNL
                     ALVGLYKIKDSSLLYKCLQHLFVEDIKTQGEYNLTDALDCMIQRGAKFQSFKVKNWFD
                     CGKKETLIESNATLLKKLGGNTTSTHQFEDTIIIPPVSIAENCHIKNAVIGPNVSIGN
                     NTSILNSVVHNSIIGSFTSIKQVVLNNSLIGSDAHVKGLSKSLNIGDNTEIEFG"
     gene            complement(1584..2231)
                     /locus_tag="PLU36_01550"
     CDS             complement(1584..2231)
                     /locus_tag="PLU36_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45464.1"
                     /translation="MKKIIIVLLMFLYSCNQNNENVSLLEDVYSIPSYMRYAASSNFS
                     ENTDAEHNYKLLDTLKWMHESKLQTWNSKKMWFVLKKEMSDTLLKTKQENVVSFAKAY
                     KALVLLKYTDLLQQQDDEAIQEMKDCLEQLIAWQYGGYHIYYATIISLKNHIPNKELK
                     GYINESLAIENPLFTVHVRDADTDDEKAVSSLLGTNKLISNIQKMKLYKEKLAIL"
     gene            2542..3483
                     /locus_tag="PLU36_01555"
     CDS             2542..3483
                     /locus_tag="PLU36_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HMZ45465.1"
                     /translation="MFTNSITSLFKIQYPIIQAGMIWASGWRLASAVSNAGGLGIIGA
                     GSMYPAVLKEHIKKCKAATNKPFAVNLPLLYPSIEEHIKIIMEEKVPIVFTSAGNPKT
                     WTSLLKENGIKVVHVVSSSKFAQKAEAAGCDAVVAEGFEAGGHNGREETTTMVLIPAV
                     KKAISIPLIAAGGIATGKQMLAAMILGADAVQIGSRFVCTPEASSHQNFKESILNANE
                     GDTMLHLKKTVPVRLLKNKFSETIKEAENRGASAEELNEILGRARAKKGMFEGDLQEG
                     ELEIGQVSSIIHEIKPAAEIVQEIWNEFETTLKNPFK"
     gene            3632..4021
                     /locus_tag="PLU36_01560"
     CDS             3632..4021
                     /locus_tag="PLU36_01560"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMZ45466.1"
                     /translation="MKNILLTITSLFLVFMVSAKTIKSKGEPVVVGNVTDAVSKKPVA
                     EVTVTAIHLTTKSEHQISTDINGNFKINQLPTGNYKIKFEKNNYKPVEKNNITIKQDS
                     TSKINIEITEKEETTYESIIWDLRFGI"
     gene            complement(4028..4372)
                     /locus_tag="PLU36_01565"
     CDS             complement(4028..4372)
                     /locus_tag="PLU36_01565"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ45467.1"
                     /translation="MKKIYILFLGIFLIVGTSFTVPFGSGNDVEIKTKIVNFYPNPAT
                     SVIYFEYTKALDKGCTIQLYNFIGRKVHEVVVTGSKTTVNLDGYYRGIYVFQLRDKSG
                     KIIESGKFQVVK"
     gene            4568..5494
                     /gene="nusB"
                     /locus_tag="PLU36_01570"
     CDS             4568..5494
                     /gene="nusB"
                     /locus_tag="PLU36_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HMZ45468.1"
                     /translation="MISRRNIRVKVMQLLYVLESSGDKNFFKDPITQLQKNINKTNEL
                     LVYLIYNLTEVIRYVEIDAKNRASKHLPSYQDLNVNTKIAGNTILWGLLENNYIKDAF
                     IQYKCSSMVNTELIRKLYLQLIETEEYKTYIANQQRDKKEDKVIVDFVFNSLMLPNEL
                     FDEHIDELFSNWDDDAELMQQLVNAFIQKPGNDLASLLPEETLSFGKLLLKTVIEKNS
                     HTLETIKPKLKNWDSDRIAVLDMILMQMGICELLYFETIPTKVTINEYIDLAKSYSTN
                     QSGQFVNGILDSIHKELLAEGKINKVDFKKKK"
     gene            5500..5934
                     /locus_tag="PLU36_01575"
     CDS             5500..5934
                     /locus_tag="PLU36_01575"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HMZ45469.1"
                     /translation="MKTVLYISLLLFIFGCGSEQTLKPKSLSDLEKIMADTTNYSNIH
                     WIDSIADFGTIKEGEVIEVKFRFKNIGNKPLYITNVQAGCGCTSPDYSKEAIAPQQEG
                     WVKGIFNSLNQVGTVSKQIKVITNSKNFSEHTLYFTGTVNNN"
     gene            5964..6299
                     /gene="yajC"
                     /locus_tag="PLU36_01580"
     CDS             5964..6299
                     /gene="yajC"
                     /locus_tag="PLU36_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HMZ45470.1"
                     /translation="MNFLQATFLMANPNQPGSQWTSLIMMGAIILVFWFFMIRPQAKK
                     AKEQKKFIDNLQKGDKIVTIAGIHGVINKVNDDGTINLEVNPGSYIKIERSAISLEWS
                     NAINKTAAK"
     gene            6871..8133
                     /locus_tag="PLU36_01585"
     CDS             6871..8133
                     /locus_tag="PLU36_01585"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HMZ45471.1"
                     /translation="MKILLKKVLIVDKESIFNNTINDILIENGIIKTINENITETAEI
                     VFQEKNCIVSIGWVDCFSNFNDPGFEFRETLETGSNAAIAGGFTTVCIIPNTQPTIHS
                     KSQVEYVVQKTKQLPINILPLGTLTQNAEGKILAEMYDMHQSGAIAFTDGLHPIQIPG
                     VLLKALQYVKSFNGTVIQMPIDKSIGGNGLMNEGIISTKLGLPGLPSLAEEIIIKRDI
                     ELLKYTQSKLHITGITTAKSVALIKGAKAEGLAITCSVTPYHLFYCDEDLQAYDTNLK
                     VNPPLRTKADVVALQQAVLNGDIDCIATHHFPHHSDNKVCEFEYAKNGMIGLQTAFAA
                     INTALPNLSNELLVNLFYTNATDIFSLRKNSIKENQVANLTIFTRDGNTTLNKENNKS
                     KSFNTPFSNKTLKGKVLGVITKNIFHKN"
     gene            8142..8660
                     /locus_tag="PLU36_01590"
     CDS             8142..8660
                     /locus_tag="PLU36_01590"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HMZ45472.1"
                     /translation="MEQKPLSVPVKGLIISLVLIVLGLIIHFAGLSQIKGLQLLQYVI
                     LGSGLIWACITYAKQNNGNVTFGNTFAHGFKVTAAITGIIAVYTFLAIKFIMPEMIDY
                     AIDEARKGMVEGNKMTAEQIEQALAMTRKFFLPFALAGVIVGFLVLGVIFSLIGAAVA
                     KKNPNYSPIQNQ"
     gene            8669..9607
                     /locus_tag="PLU36_01595"
     CDS             8669..9607
                     /locus_tag="PLU36_01595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ45473.1"
                     /translation="MDISIVIPLYNEEESLPELSEWIKRVTTTNNFSYEVIFIDDGST
                     DNSWNIIEKINANNNNFKGIKFQRNYGKSAALNEGFRAAKGTVVITMDADMQDSPDEI
                     PSLYKMVTEDNFDIVSGWKKKRFDPITKTIPTKFFNAATRWSSGIKLHDFNCGLKAYK
                     NKVVKSIEVYGEMHRYIPVIAKWAGFRKIGEKIVEHRTRKYGKTKFGWERFINGFLDL
                     ATISFVSRFSKRPMHFFGLYGTICFLCGFLMSIYLIISKLLISNFALTNRPAFFIALT
                     VMIIGMQLFLAGFIAELIGRNAPERNSYLIESRLDV"
     gene            complement(9625..10326)
                     /locus_tag="PLU36_01600"
     CDS             complement(9625..10326)
                     /locus_tag="PLU36_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HMZ45474.1"
                     /translation="MLFYAKTYLADFLHLIFPNYCEGCGTDNITEDHFLCIKCIAALP
                     VTGFENVYENTIEKMFYGRLNLQYATAGFYFSKQTLMQHLMHQLKYKGNKEAGLFLGK
                     MLGLQLLHSDKFKTIDAIIPMPISSKKIVVRGYNQAEIISQGITMVFNKPILKNVVVR
                     IINSKTQTKQNRVERWENMKGVFEIKNKIDLEGKHILLVDDIVTTGATLEACGKVILQ
                     NGNCTLSIATVAYTL"
     gene            complement(10330..11694)
                     /gene="radA"
                     /locus_tag="PLU36_01605"
     CDS             complement(10330..11694)
                     /gene="radA"
                     /locus_tag="PLU36_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HMZ45475.1"
                     /translation="MAKIKTSFFCNNCGHESTKWLGKCPSCNEWNTFIEEVIEKNKSE
                     NWNSYSDDKKQNKIVALNNVSTVKENRIVTTDAELNRVLGGGIVHGSIILVAGEPGIG
                     KSTLFLQHGLMMKDITILYISGEESEQQIKMRADRLKMTNNNFYLLTETNTQIIFQEI
                     KKLKPQLVIIDSIQTLQSNLIDAGAGSVSQIKECAAEFQRFAKETNTPVFLIGHITKD
                     GNIAGPKILEHMVDTVLQFEGDRHYAYRILRTIKNRFGSTAELGIYEMTGEGMRAVNN
                     PSEILISQRDDQLSGIAIAATIEGMRPLLIEVQALVTQSVYGTPQRTVTGFDLRRLQL
                     LLAVLEKRGGFLFGTKDVFVNIAGGLKIEDPSIDLAVICALLSSYDDTPISHKICFAG
                     EVGLNGEIRAVNRIEQRIAEAEKLGFEKIFVSKFNKKSVNLKLFNIDIIMVSQLHEVY
                     QKIF"
     gene            complement(12069..13616)
                     /locus_tag="PLU36_01610"
     CDS             complement(12069..13616)
                     /locus_tag="PLU36_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HMZ45476.1"
                     /translation="MNKELIINTTPIGVEIALLEDKKLVELHNEKSDANFGVGDLYLG
                     KVKKLIPGLNAAFVDVGFEKDAFLHYTDLSPFARSILKFTQQAMAANENSFEFSNFQI
                     EPEIIKSGKINEVLNGKPNVLVQILKEPIAAKGPRLSCEISLPGRFIVLTPFNEIVAV
                     SKKIHSGEERKRLQKIVESIRPKNFGVIVRTAAEGKSTAELHQDLLELTETWKTIQNN
                     IKGAVAPAKILSEQNKTTSILRDLLNESFNKIVVNDKNIFNDIKSYIQRIAPDKTDIV
                     NYVNNGTPLFDQFGITKQVKGAFGKTVNLDSGAYLIIEHTEALHVIDVNSGYKSVSNS
                     QEENALQTNLEAAEEIARQLRLRDIGGIIVVDFIDLKLPDNRKKLQEAMEEYLKADRA
                     KHAVLPISKFGLMQITRQRMRPEVNINTSENCPTCSGTGKVSSTLLLEDEIEKRIHYL
                     STHSHNYLNLFVHPIVHSHLTKGIFTSIVKKWRRKHKLKLTVKPNTSYGLVEFKFFDK
                     NEEEIKF"
     gene            complement(14014..14865)
                     /locus_tag="PLU36_01615"
     CDS             complement(14014..14865)
                     /locus_tag="PLU36_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ45477.1"
                     /translation="MNKKQLTLLLSGLVLFSTLFFFGKTVSPKKIKSNTEQHSPDDGH
                     GHTINFSELLIKAKEKINDEKKQYVGMLENSIANSSKEEQIKTYKLLANFWKDTARVF
                     EPYAYYTAEGAKLENSEKSLTFAAQLFIDNLFDEANASMQNWLASNGKVLLEKALAIN
                     PNNDSSKIGLGACYMLGNISDNPMQGIIEVKAVADKNPDNLYAQWVLGLGGKKSGQYD
                     KAAERFLFIVQKQPTNLIAMLHLAECYELNNNKAEAIKWYKTVQSVIPNPEAKKELQQ
                     RIEQLQK"
     gene            complement(14893..15180)
                     /locus_tag="PLU36_01620"
     CDS             complement(14893..15180)
                     /locus_tag="PLU36_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HMZ45478.1"
                     /translation="MRKSDLINNISEKTGIPKVDVLVTVETLLKEIKQSLISGENIYI
                     RGFGSFITKKRAAKIGRNIKKNSAVHIPEHYIPAFKPAKEFVSEVKTNYKP"
     gene            15379..16461
                     /gene="mutY"
                     /locus_tag="PLU36_01625"
     CDS             15379..16461
                     /gene="mutY"
                     /locus_tag="PLU36_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HMZ45479.1"
                     /translation="MQTSKEKYFTKQLLNWNKKFNDRKMPWKGIKDPYKIWLSEVILQ
                     QTKVEQGIHYYIKFTEKYTNIISLANAKDKEVYKLWEGLGYYNRCKNLLFTARKLRDE
                     NAGKFPTNYHEILQLKGIGVYTAAAIASFAFNLPYAVIDGNVYRILARYFGIHTPIDS
                     NEGKKEFTHLANKLLDKRKPAVYNQAIMDFGATVCKPNLPLCNNCKLKKNCIAFISNE
                     ANLLPIKNKRIVKKHRWLNYFILINNHDVWIKERTEKDIWQNLHEFYLVESEKKIKWN
                     ELNINKQLQQLNIKNYTIKNISKEFKQQLTHQSLHVQFIQILISTKPTTLKRDYWISQ
                     NKLSTVAFPKIINEFIKNKDNYLTSS"
     gene            16559..16981
                     /gene="ssb"
                     /locus_tag="PLU36_01630"
     CDS             16559..16981
                     /gene="ssb"
                     /locus_tag="PLU36_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HMZ45480.1"
                     /translation="MRGVNKVMLIGNLGKDPHIDYLEGNIGVAKFPLATTETYKDRNG
                     KLVSQTEWHTVVLWRGLADLASKYLHKGSLIYLEGRLRTRSWEDKEGNKKFATEIVGD
                     NLIMLDKRNEGSHSANDIIDDGYNDNQSLPNTDENLPF"
     gene            17059..18387
                     /gene="gldE"
                     /locus_tag="PLU36_01635"
     CDS             17059..18387
                     /gene="gldE"
                     /locus_tag="PLU36_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HMZ45481.1"
                     /translation="MNDFSIFFKPTLVTIIDSQGTTILFVLLLFLLVLSFILSGAEVA
                     FFSLNYKDISVLKTKQQPVYKRIVTLLENPKTLLGSLLIANTFSNISIIIISNVLLDN
                     LIHFHPNFEWVEFLIKVTLVTFMLVLFSEVMPKVMATQNNVRFAKDVGWLVEIVSYVF
                     NSLSNWLVKYSDLVEKKLSNKNLNNNTNEELYHAIDITHTDSNESEKNILKGILKFGN
                     TTVKQVMKTRLDVHGIDYNISFSELLQLINELHYSRLPVFKDDLDEIAGIIHTKDILQ
                     YLDADNNFNWHSLLRQPYFVHEQKMIEDLLSEFQSKRIHFAIVVDEFGGTSGIVTMED
                     IMEEVIGEIADEFDDEDAGQKKIDDRNYIFEGKTMIRDVCKFMNLSIDTFDGIKGESD
                     SLAGLILEIAGEIPYVTQVITCGDFEFTMLEVAKNRINKIKVTIVPHMVE"
BASE COUNT         6663 a         2673 c         2693 g         6464 t
ORIGIN      
        1 aatttgtttt aatctgtcaa ttgctaattg ttcagtttgg tcaacaattt cacaaccgcc
       61 ataatacctt cttcctggat aaccttcagc atatttatta gtcattacac cacccatagc
      121 ctgcattact tgaaggcttg taaaattttc gcttgctatt aattctattc cacgtctttg
      181 tctttgcagt tcttggttta ttaaatcaaa aacttgtgta tctctttgca taatttctac
      241 ttttaaaaat taagatgttt tgctgaaaat atttaagtat gcaaatttaa ttgattgatt
      301 gaagtaggca agtttaatta gcaaattgat ttaacgaata ctttatttta ttttttgcgt
      361 acttatgatg actggaaatt tacaaataac cacatagtgc aattaaattt ttattcctta
      421 atacaaacaa tttataaatt gaagtaaaaa aaggggtatt gatttaaaac aaaaaacttg
      481 tataaaataa ttattttcct actttcacag ccccaattat attttgtggc aaatgtttat
      541 gtgttgtaat gggaaaattt taaaaaatga aagcaatcat ccccgttgct ggtgcaggaa
      601 ccaaacttcg tcctctaacc tatacacagc caaaagcttt aataccttta gcaggtaaag
      661 caatacttgg ttttattgtg gatcagctaa aaaatgctgg tataaatgaa tttattttta
      721 tagttggata ttttggcgat aaaattcaag attacataaa acaaaattac ccatctataa
      781 atgcacactt tgtttatcaa aacgaaaggc atggtactgg gcatgcaatt gaattaacca
      841 ggcatattgt ggatagcgat gaggttttta ttgccttggg agatacaata tgtgaatatg
      901 atttaaacga agtaatacat agcccgttta atatgttagg ggtaagaaaa attgatgacc
      961 caagaaattt tggagttgca accataaacg aagaaggctt tattgaaaat gttgtagaaa
     1021 aaccagcatt tccgaaaagt aatttggctt tagtgggatt atataaaatt aaagacagca
     1081 gcctattata taaatgcctg caacatttat ttgttgagga tataaaaacg caaggtgaat
     1141 ataatttaac cgatgcctta gattgtatga tacagcgtgg agcaaagttt caaagtttta
     1201 aagttaaaaa ctggttcgat tgtggtaaaa aagaaacatt aattgaaagc aatgctactc
     1261 ttttaaaaaa acttggtggc aatactactt ctacccatca atttgaagat actattatta
     1321 tacctcctgt aagtattgct gaaaattgcc acataaaaaa tgctgtaatt ggtcccaatg
     1381 tctctattgg taataacact tctatattaa actctgttgt acataatagt attattggct
     1441 catttacatc tattaaacaa gtagttctaa ataattcatt aattggtagc gatgcccatg
     1501 ttaaaggatt aagcaaaagt ttaaatattg gcgataatac agaaatagaa tttggataga
     1561 gtattttaat aatgccagta ttattataga atggcaagtt tttctttata caatttcatt
     1621 ttttgaatat tgcttattag tttgtttgta cctaacaagc tagatactgc cttttcatca
     1681 tcagtatcag catctcttac atgaacagta aacaatggat tttcaatagc taaactctcg
     1741 ttaatatatc cttttaactc tttattaggt atatgatttt taaggcttat aattgtagca
     1801 tagtaaatat gataaccacc atattgccat gcaataagct gttccaaaca atctttcatt
     1861 tcctgtattg cttcatcatc ttgttgctgc aaaagatctg tatatttaag taatacaagt
     1921 gctttgtagg cttttgcaaa tgaaacaaca ttttcttgtt ttgttttcaa taatgtatca
     1981 ctcatttctt tttttaaaac aaaccacatt tttttagaat tccacgtttg caatttgctt
     2041 tcatgcatcc atttcaaagt gtctaataat ttgtaattat gttcagcatc tgtattttct
     2101 gaaaaattgc tactagcagc ataacgcata taagatggaa tggaataaac atcttctaat
     2161 aggcttacat tttcattgtt ttgattacaa ctataaagaa acattaatag aacaataatt
     2221 atttttttca taattaattg ttttaagtta gttaataatg taatgaatgg catttgcata
     2281 cgaaagaaaa aaaaaaaatg acatttccaa atatattttt ttgaagccca caaagggttt
     2341 ggtgcaaaaa tattgtactt tacttcctca atttaattag tttcattaat gaagaattct
     2401 ttcaattagt tattaatttt atcgaaattt gctttaacat tgtacaggtt gattgttttt
     2461 attaagccat gtagtagcaa tatttaaaac attatttcaa aaacttattt tcactaaaaa
     2521 ttaatttatt ttttatcatt tatgtttacc aatagcatta catctttatt caaaattcaa
     2581 tatcccatta tacaagcagg aatgatttgg gcaagtggtt ggcgtttagc aagtgctgta
     2641 agtaatgcag gtggtttagg tattattggt gcaggaagta tgtatcctgc tgttttaaaa
     2701 gagcatatta aaaaatgtaa agcagcaacc aataaacctt ttgcagtaaa cttgcctttg
     2761 ctttaccctt ctattgagga acatataaaa ataattatgg aagaaaaagt tccgattgtt
     2821 tttacaagtg ccggaaatcc taaaacatgg actagcttat taaaagaaaa tggaattaaa
     2881 gtagtgcatg tagtaagcag tagcaagttt gcacaaaaag cagaagcagc aggctgtgat
     2941 gctgttgtag cagaaggttt tgaagcaggt ggacataatg gaagagaaga aacaaccaca
     3001 atggttttaa ttcctgcagt aaaaaaagca attagcattc ctttaatagc agcaggtggt
     3061 attgcaacag gtaaacaaat gttggctgca atgattttag gtgcagatgc tgtacaaata
     3121 ggcagtaggt ttgtttgtac tcctgaagca tccagtcatc aaaattttaa agagtcaatt
     3181 cttaatgcca acgaaggcga tactatgttg catttaaaaa aaacagttcc tgtacgttta
     3241 ttaaaaaata aattttctga aaccattaaa gaagcagaaa atagaggagc ttcagccgaa
     3301 gaattgaatg aaattttagg cagagcaaga gccaaaaaag gaatgtttga aggtgactta
     3361 caagagggtg aattagaaat aggacaagta agcagcatta tacatgaaat aaaacctgct
     3421 gcagaaattg tacaagaaat ttggaatgaa tttgaaacta ctttaaagaa tccttttaaa
     3481 taataaccat ctaaatttaa gttctatatc tacaatactt ttttcatttg caaaatgtta
     3541 aaatttgcaa aacatttaac catttacaca aaaggtagta atagataaag aaaaaaaatt
     3601 aactttcaat cttaatttca aaaattaatt tatgaaaaat atattactta ctattaccag
     3661 cttattttta gtatttatgg ttagtgctaa aactataaaa agtaaaggag aacctgttgt
     3721 agtaggtaat gtaacagatg cagtttccaa aaagccagta gcagaagtta ctgtaacagc
     3781 aattcattta actactaaat cagagcatca aattagcacc gatataaatg gaaattttaa
     3841 aataaatcaa cttcctacag gcaactacaa aataaaattc gaaaaaaaca attataagcc
     3901 tgtagagaaa aataatataa cgattaagca agatagtact tcaaaaataa atattgaaat
     3961 taccgaaaaa gaagaaacaa cctacgaaag tattatatgg gatttgaggt ttggtatata
     4021 acacaaacta tttaacaacc tgaaattttc cactttcaat tattttacca ctcttatctc
     4081 ttaattgaaa aacataaatt cctctgtaat agccatctaa attaacggtt gttttactgc
     4141 cagttaccac cacttcatgt actttacgac caataaaatt atacaattgt atagtacaac
     4201 ctttatctaa agctttagtg tattcaaaat aaataacaga tgttgccgga ttgggataaa
     4261 aatttacaat tttagtttta atttctacat cattaccact accaaatggc acagtaaaag
     4321 atgtgccaac aattaaaaaa atgcctaaaa atagaatgta gatttttttc atattaaata
     4381 tacatttatg aaggtaaata aacattttaa atttcccaaa acttatttgt ttaacagcta
     4441 acgaaaattc aaaaaaaata ttatgtaaca ttaaataata acaatatttt ttttaatcaa
     4501 aactttaact attaagccaa taaaccccct tatcttcgca gccctaaaat aatgctcttt
     4561 atattaaatg attagtagaa gaaatatacg ggttaaagta atgcagcttt tatatgtttt
     4621 agaaagcagt ggcgataaaa acttttttaa agaccctata acacaattac aaaaaaatat
     4681 taataaaaca aacgaactat tggtgtactt aatttataac ctaactgagg ttataagata
     4741 tgtagaaatt gatgcaaaaa acagagctag taaacacctt ccatcttatc aagatttgaa
     4801 tgtaaatact aaaattgctg gcaatactat attgtggggt ttacttgaaa acaattatat
     4861 taaagatgca tttatacagt ataaatgttc tagtatggtt aatacagagc ttattagaaa
     4921 attgtattta cagctaatag aaacagaaga gtataaaacc tatatagcta atcaacaaag
     4981 agataaaaaa gaagacaaag taattgtgga ctttgtattt aatagtttaa tgttacctaa
     5041 tgaattgttt gatgaacata tagatgaact tttttctaac tgggatgacg atgcagaatt
     5101 gatgcaacaa cttgtaaatg cctttataca aaagcctggc aatgatttag caagcttgtt
     5161 acctgaagaa actttaagtt ttggcaaatt gcttttaaaa actgttattg aaaaaaacag
     5221 ccatacttta gagacaataa aaccaaaatt aaaaaattgg gatagcgata gaattgcagt
     5281 tttagatatg attttaatgc aaatgggtat atgtgaactt ttgtactttg aaacaattcc
     5341 aactaaagtt actattaacg agtatattga tttggcaaaa agttatagca ctaaccaaag
     5401 tgggcaattt gtaaacggaa ttttagatag tattcataaa gaattactgg ctgaaggaaa
     5461 aataaataaa gttgatttta aaaagaagaa ataaaattaa tgaaaacagt attgtacata
     5521 agtttactac tttttatttt tggttgcggg tcagagcaaa ctttaaagcc taaaagctta
     5581 tcagatttag aaaaaattat ggccgatact actaactatt caaacataca ttggatagat
     5641 agtatagcag attttggtac cataaaagaa ggagaagtga tagaagtgaa atttagattt
     5701 aaaaatattg gtaacaagcc tttgtatata accaatgttc aggcaggttg tggttgcacc
     5761 tcaccagatt attcaaaaga agcaattgca ccacaacaag aaggttgggt aaagggaatt
     5821 tttaatagct taaaccaagt tggcactgta agtaaacaaa ttaaagtgat aaccaactcc
     5881 aaaaactttt cagaacatac actctatttt acaggaacag taaataataa ttaataattt
     5941 ttcaacttaa aaaacaaaac agtatgaatt ttttacaagc aacattttta atggctaatc
     6001 ctaatcagcc aggcagccaa tggacttctt taataatgat gggagccata attttagtat
     6061 tttggttttt catgattcgt ccacaagcaa aaaaagccaa agaacaaaaa aagtttattg
     6121 ataacttaca aaaaggcgat aaaatagtaa ccattgcagg catacatggt gtaataaata
     6181 aagtaaatga tgatggcact attaacttgg aagtaaaccc tggtagttat attaaaattg
     6241 aaagaagtgc aataagttta gagtggagta atgcaataaa caaaactgca gccaaataat
     6301 ttattaaata aaaaaagttg tattactgcc tccaatttgt gaggcagttt tttttgtcat
     6361 gaatttgttt tactgatata gtaattggat ttccttaatt tataaaagtt atactaacgc
     6421 ttaaaataca tttgtgttct ataaataatt ataacagtac tatgaaagca attttattaa
     6481 agaataaaat gttatttagt tatcttatta aattttttat ttggcttagt ttaattattg
     6541 ctgttaaagc tattctattg cttttattgc cattaattat tttttaattt ctatttaaac
     6601 tctggtggtt taataactcc actaacttta ataaagttac ctttattttc tgctacttta
     6661 tagtagcagt ttttatataa atacagctta ttctttataa ttcacaaccc atattcggtt
     6721 catagcttat taattaatta ttaattttat attttttact attcatttct acaattaaag
     6781 gttgaaaaag tgattaaaat tcattactct taaatctaaa cacttaattt aatttagatt
     6841 aatattgcat aaatttcttg taaatcactt atgaaaattc ttctcaaaaa agttttaata
     6901 gttgataagg aaagtatttt taataatacc attaacgata ttttaattga aaatggaatt
     6961 attaaaacaa ttaatgaaaa cattaccgaa actgcagaaa ttgtttttca agaaaaaaat
     7021 tgcatagtat ctataggttg ggttgattgt ttctccaact ttaacgaccc tggttttgaa
     7081 tttagagaaa cattagaaac aggaagtaat gcagctatag caggtggatt tacaactgta
     7141 tgtattattc caaataccca accaactatt cattcaaaat cgcaagtgga atatgtagtt
     7201 cagaaaacaa agcaattacc aataaatatt ttgccattag gcacattaac ccaaaatgca
     7261 gaaggcaaaa ttttggctga aatgtatgat atgcaccaaa gtggtgccat tgcatttact
     7321 gacggtttac atcctattca aatacctggt gttttactaa aagcattaca atatgtaaaa
     7381 tcttttaatg gtactgttat tcaaatgcca atagataaaa gtattggtgg taatgggcta
     7441 atgaatgaag gaattatttc aactaaatta ggtttaccag gtttaccaag tttagcagaa
     7501 gaaataataa ttaagagaga tatagaatta ctaaaataca cccaaagtaa actacatatt
     7561 acaggtataa caactgctaa aagtgtagca cttataaaag gagctaaagc agaaggcttg
     7621 gcaattactt gtagtgttac accatatcat ttgttttatt gcgatgaaga cttgcaagcg
     7681 tatgatacaa atttaaaagt aaaccctcct ttgcgtacta aggcagatgt ggttgcacta
     7741 cagcaagcag ttttaaatgg agatattgat tgtattgcca cacatcattt tccacatcat
     7801 tcagataata aagtgtgtga atttgagtat gcaaaaaatg gtatgattgg cttacaaaca
     7861 gcttttgcag ccattaatac tgcacttcca aatttatcta atgaactgct ggtaaattta
     7921 ttttatacta atgcaactga tatttttagt ttaagaaaaa atagcattaa agaaaatcaa
     7981 gttgctaact taactatatt tacaagagat ggaaatacta ctttaaataa agaaaataat
     8041 aaaagcaaat cattcaatac ccctttttca aacaaaacct taaaaggaaa agtattgggc
     8101 gtaattacaa aaaacatttt tcacaaaaac taaaataaaa aatggaacaa aagccactat
     8161 ctgtacctgt taaaggatta atcatttctt tagtattaat tgtattaggt ttaattatac
     8221 actttgcagg cctaagtcaa attaaaggat tgcaactttt gcagtatgta attttagggt
     8281 ctggtttaat ttgggcatgt ataacttatg caaagcaaaa taatggtaac gttacttttg
     8341 gtaacacatt tgcacatggc tttaaagtta ctgctgccat tactggtatt attgcagttt
     8401 atacatttct agctattaaa tttattatgc cagaaatgat agattatgct attgatgaag
     8461 caagaaaagg aatggttgaa ggaaataaaa tgactgcaga acaaatagaa caagcattgg
     8521 caatgactag aaaattcttt ttaccttttg ctttagctgg tgttattgta ggctttttag
     8581 ttttaggagt aatattttct ttaattggtg cagcagttgc taagaaaaat ccaaactact
     8641 cccctattca aaatcagtaa aatactatat ggatatttct attgttatcc ctttatacaa
     8701 cgaagaagaa tcgctacctg aattaagtga atggataaaa cgtgtaacca ctacaaataa
     8761 ttttagttat gaagtaattt ttattgatga tggtagcacc gataatagtt ggaatattat
     8821 tgaaaaaata aacgctaaca acaacaattt taaaggaata aaatttcaac gaaattatgg
     8881 taaaagtgca gctttaaacg aaggttttag agctgctaaa ggaactgttg taataactat
     8941 ggatgcagat atgcaagaca gtccagatga aattccttct ttatacaaaa tggttactga
     9001 agataatttt gatattgtta gcggttggaa gaaaaaacgc tttgacccta ttactaaaac
     9061 aattccaaca aaatttttta atgcagccac acgttggagt agtggcatta aactacacga
     9121 ttttaattgt ggcctaaaag catataaaaa caaagtagta aaaagcattg aagtatatgg
     9181 cgaaatgcac agatatattc ctgtaattgc taaatgggct ggcttcagga aaattggcga
     9241 aaaaattgtt gagcatagaa ctagaaaata tggtaaaaca aaatttggat gggaacgatt
     9301 tattaacggc tttttagatt tagcaaccat aagctttgtt agcagattta gtaaaagacc
     9361 aatgcacttt tttggacttt atggaacaat ttgtttctta tgcggatttt taatgagcat
     9421 ttatttaatt atttcaaagc tcttaataag caactttgca ttaaccaatc gtcctgcttt
     9481 ttttattgca ttaacagtaa tgataattgg tatgcaactt tttttagcag ggtttattgc
     9541 cgaattaatt ggaagaaatg cacctgaaag aaatagttat ttaattgaaa gtagattaga
     9601 tgtttaatac tgcattttta tacactataa agtataagct acagttgcaa tacttaatgt
     9661 gcaattgcca ttttgtaaaa taactttacc gcatgcttct aaagtagcac ctgttgttac
     9721 aatatcatct actaataaaa tatgtttccc ttctaaatca attttatttt ttatttcaaa
     9781 aacacctttc atattttccc aacgttctac cctattttgt ttggtttgag tttttgaatt
     9841 aataattctt actacaacat tttttaatat tggtttatta aaaaccattg taatgccctg
     9901 cgaaataatt tctgcctgat tgtagcctct tacaactatt ttttttgagc ttatgggcat
     9961 tggaataatt gcatctatag ttttaaattt atccgaatgt aacaattgca gcccaagcat
    10021 ttttcctaaa aataagcctg cttctttatt acctttatac tttaattggt gcattaaatg
    10081 ttgcattaaa gtttgttttg aaaaataaaa accagcagtt gcatattgta aatttaatct
    10141 gccataaaac attttttcaa ttgtattctc atacacattt tcaaaaccag taactggcaa
    10201 tgcagcaata catttaatac ataaaaaatg atcttctgta atattatcag tgccacaacc
    10261 ctcgcaataa ttagggaaaa taagatgcaa aaaatcagct aaatatgttt ttgcataaaa
    10321 taacatacac taaaatattt tttgataaac ttcgtgcaat tggcttacca taataatatc
    10381 tatattaaat agttttaagt taaccgattt tttgttgaat ttactaacaa atattttttc
    10441 aaatccaagt ttttctgctt cggcaattct ttgttcaatt ctgtttacag ctctaatttc
    10501 tccattcaat cccacttctc ctgcaaagca aattttatgt gaaataggcg tatcatcata
    10561 actggatagt aaagcacaaa taactgctaa gtcaatggat gggtcttcta tttttaaccc
    10621 tcctgcaatg tttacaaaaa catcttttgt gccaaataaa aaccctcctc gtttttctaa
    10681 aacagcaagt aacaattgta acctacgcaa atcaaagcct gtaacagtac gttgtggggt
    10741 gccataaaca ctttgtgtaa ccaatgcttg cacttctatc agcaatggtc tcattccttc
    10801 tatagttgca gcaattgcaa taccacttaa ttgatcatct cgttgagaaa ttaaaatttc
    10861 gcttgggtta tttactgccc tcatgccttc tcctgtcatt tcataaatac caagttctgc
    10921 agtgcttcca aatctatttt taatagttct taaaattctg taggcataat gtctgtctcc
    10981 ttcaaattgt aaaacagtat ctaccatgtg ttctaaaatt ttaggccctg caatgttgcc
    11041 atcttttgtt atatggccaa ttaaaaaaac gggtgtattg gtttcttttg caaatcgttg
    11101 aaactctgca gcacattctt ttatttggct tacactacct gcacctgcat ctattaaatt
    11161 gctttgtaat gtttgtatag aatctataat aacaagttgt ggttttaatt tttttatctc
    11221 ttgaaaaata atttgtgtat ttgtttcggt aagtaaataa aaattattgt tagtcatttt
    11281 tagcctgtct gctctcattt ttatttgttg ctcactttct tccccactaa tgtacaatat
    11341 tgttatatcc ttcatcatta atccatgttg taaaaacaat gtactcttcc caattccagg
    11401 ttcacctgca acaagtatta tactaccatg tacaattcca cctcctaata ctctgtttaa
    11461 ctcagcatca gttgtaacaa ttctattttc ttttacggtg gatacattat ttaatgcaac
    11521 aattttattt tgctttttat catcagaata actattccag ttttctgatt tatttttttc
    11581 aatcacttct tctataaatg tattccattc attacaactt gggcacttac ctaaccattt
    11641 tgtactttca tgaccacagt tgttacaaaa aaaagatgtt ttaatttttg ccatagtata
    11701 aagataacaa tcgtactaat ttatgtttta ttgaataaaa aatttgtgta agaaaaataa
    11761 aagaagaaaa aagttaggct aaaagaagaa gaattaattc tcattttcaa tttagaatca
    11821 acttactagt gccaacatta ctaaatgtaa aagggtcggc atttctgcta accctcttac
    11881 ttactaaccc attaaattct aagtgctaaa ttacaattta gggcggtaat tcaaaatatt
    11941 tttttgtatt tgtgtaaaac ttacttgttt aaagtataga ttttaaaaat tttacagcta
    12001 aaatacacaa aaacatgaca cttggtctta attttattcc acaaataata aattaagcca
    12061 ttttgtcgct aaaatttaat ttcttcttca ttcttatcaa aaaacttaaa ttctactaat
    12121 ccgtagcttg tatttggctt aactgtaagt tttaatttat gctttctcct ccattttttt
    12181 acaatagaag taaaaattcc ttttgtaaga tgagaatgta caattggatg aacaaataga
    12241 tttaaatagt tatgagaatg agtagataaa taatgaattc ttttttcaat ttcatcttct
    12301 agcaacaagg tactacttac cttgccagtt cctgagcatg ttggacaatt ttctgaagta
    12361 ttaatattta cttcaggcct cattctttgc cttgtaattt gcattagccc aaatttagaa
    12421 attggtaaaa ctgcatgttt ggctctatcg gcttttagat attcttccat agcttcttga
    12481 agcttctttc tattatctgg aagtttcaaa tcaataaagt caacaacaat tattcctcca
    12541 atatctctta accttagttg tcttgcaatt tcttcggctg cttctaaatt tgtttgcaaa
    12601 gcattttctt cctggctatt gcttacactt ttataaccac tatttacatc tataacatgc
    12661 aatgcctctg tatgttctat tattagatat gcaccactat ctaaattaac agttttgcca
    12721 aatgcacctt ttacttgttt agtaattcca aactgatcaa acaatggtgt gccattgttt
    12781 acataattta caatatccgt tttatctggt gcaattcttt gaatgtagct tttaatatcg
    12841 ttgaagatat ttttatcatt aacaactatt ttattgaaag attcatttag taaatctctt
    12901 aatatgcttg ttgttttatt ttgttcactt aagattttgg caggtgctac agctccttta
    12961 atattatttt gaatagtctt ccaggtttct gttaactcta gtaaatcctg atgtagctca
    13021 gcagttgatt taccttcggc agctgttcta acaattacac caaaattttt aggccttata
    13081 ctttcaacaa ttttttgtaa cctttttctt tcttcgccag aatgaatttt tttagatact
    13141 gcaacaattt cattaaatgg tgttaatact ataaaacgcc caggcaggct tatttcacaa
    13201 cttaatctag gaccttttgc ggcaattggc tcttttaaaa tttgcactaa tacatttggc
    13261 ttgccattta atacttcatt aatcttacca gatttaataa tttctggttc tatctgaaaa
    13321 ttagaaaatt cgaagctatt ttcattagca gccattgctt gctgggtaaa ttttaaaata
    13381 gaacgtgcaa atggagacaa atctgtataa tgcaaaaaag cgtctttttc aaaaccaaca
    13441 tctacaaagg cagcatttag cccaggtatt aattttttta cttttcctaa atataaatct
    13501 ccaactccaa aatttgcatc agacttttca ttatgcaact caaccagttt tttatcttca
    13561 agcaatgcaa tttctacacc aattggcgta gtattaatta ttaattcttt attcacaaaa
    13621 tttgcaactt atttaaatac acaatcactc cttacttaaa agaagtatta caacagcact
    13681 atttagtaat tagccagaaa gaaaaaggga ggaaaggtaa agttgcagca aggaaggcta
    13741 ataccttaaa gtgtttgctt tttaaaagcc tgtatataat ttaaaatata cagactttta
    13801 taagcttggc aaatgccaat cattcattta gaagaaatat tatttcttct tcttatgacg
    13861 attttttctt aaacgttttt tacgtttgtg cgtagcaatt ttatgacgct ttctcttttt
    13921 accacaaggc ataaattcaa ataattttaa ccagttaaaa aataaaataa attataaaac
    13981 ctgtatttag tttttaaacc tatacagcag tttttatttt tgcaattgtt caattctttg
    14041 ttgaagttct tttttagctt caggattggg tataacagat tgaacagttt tataccattt
    14101 tattgcttct gctttattgt tatttaattc ataacattca gctaaatgca acatagctat
    14161 taaattagtt ggctgttttt gtactataaa taaaaatctt tctgctgctt tatcgtattg
    14221 tccacttttc tttcctccta aacctaatac ccattgtgca tacaaattat ccggattttt
    14281 atcagctact gctttaacct ctataattcc ttgcattgga ttgtctgaaa tatttcctaa
    14341 catataacag gcaccaagcc ctattttgct actatcgtta ttagggttta ttgccaatgc
    14401 tttttctaaa agaacttttc cattgctagc taaccagttt tgcatgcttg catttgcttc
    14461 atcaaacaag ttatcaataa acaattgggc ggcgaaggtg aggctttttt ctgaattttc
    14521 caattttgct ccttcagcag tataataagc ataaggttca aacactctag ctgtatcttt
    14581 ccaaaaatta gctaatagtt tataagtctt aatttgttct tctttactgc tattggcaat
    14641 gctgttttca agcattccta catattgttt tttttcgtcg ttaatctttt cctttgcttt
    14701 tattaatagt tcagaaaagt ttattgtatg gccatgccca tcatctggag aatgctgttc
    14761 tgtattgctc ttaatttttt tgggagaaac tgttttacca aagaaaaata aagtagaaaa
    14821 tagaactaaa ccacttaata gtaaagttaa ttgcttctta ttcacttaaa aaatttgcct
    14881 caaaggtaac agttatggct tatagtttgt tttaacttca gaaacaaatt ctttggcagg
    14941 tttaaatgct ggtatataat gctcgggtat atgaacagca gagtttttct taatatttct
    15001 accaattttt gctgctcttt ttttggtgat aaaactacca aaacctctaa tataaatatt
    15061 ctcgccagag ataaggcttt gttttatctc tttcaatagt gtttctacag ttactaacac
    15121 atctactttt ggaatacctg ttttttcaga tatgttattg attaaatctg attttctcat
    15181 aagttaattt tttcatgtgt aagcaatcaa agtctaaagt aaataaaact atttggtatt
    15241 tacaagtatt tttttgaaaa catgaacaca ttattatacc ttttttaaag aaatctaaaa
    15301 tactaaacct aaaaactaat tgaataatta aataattaaa gttttatttg tataaacagc
    15361 tttgctatat acctttttat gcaaacaagt aaagaaaaat attttaccaa acaattatta
    15421 aattggaata aaaaatttaa cgatagaaaa atgccttgga agggcattaa agacccttat
    15481 aaaatttggc ttagtgaggt tattttacaa caaacaaagg ttgaacaagg catccattat
    15541 tatataaagt ttacagaaaa atatacaaat ataatttcat tggcaaatgc aaaagataag
    15601 gaagtatata aactatggga aggcttaggt tactataaca gatgtaaaaa tttattattt
    15661 actgcaagaa aactaagaga tgaaaatgca ggaaaatttc ctacaaatta ccatgaaatt
    15721 ttacaattga aaggaattgg tgtttacaca gcagcagcta tagcatcatt tgcatttaat
    15781 ttaccttatg cagttattga tggtaatgtg tatagaatat tggctcgcta ttttggaata
    15841 cacactccta ttgatagtaa tgaagggaaa aaagaattta cacacttagc aaacaaatta
    15901 ttagataaac gtaaacctgc agtttacaac caagcaataa tggattttgg agcaaccgtt
    15961 tgtaagccta atttaccctt atgtaataat tgcaagctta aaaaaaattg tattgctttt
    16021 attagtaacg aggcgaattt attgccaata aaaaataaaa gaattgtaaa aaaacataga
    16081 tggcttaact actttatact aattaataac catgatgttt ggattaaaga aagaacagaa
    16141 aaagatatat ggcaaaactt acatgaattt tatttagttg aatctgagaa aaaaataaag
    16201 tggaatgaat taaatataaa taaacaattg cagcaactaa acattaaaaa ttatactatc
    16261 aaaaatattt ctaaagaatt taaacaacaa ctcactcacc aaagtttaca tgttcaattt
    16321 atacaaatat taatttctac taaacctaca actttgaaaa gagattattg gatatctcaa
    16381 aacaaacttt caacggtggc tttccctaaa ataataaatg agtttataaa aaacaaagac
    16441 aactacctta ctagtagcta agcataaaaa taaatatgga taaaaaagtg gttcatatcg
    16501 taaaaaaaat tattttagaa caagaaatta atttttttat aaaaaataaa aaaaagctat
    16561 gagaggagtt aataaagtta tgttgatagg aaacttaggt aaagacccac acattgacta
    16621 cttggaagga aatattggag tggcaaaatt tcctttagct acaacagaaa cttataaaga
    16681 cagaaatgga aaattagtga gccaaacaga atggcatact gttgttttat ggcgtgggtt
    16741 agccgattta gctagtaaat atttacacaa aggaagctta atatatttag aaggacgttt
    16801 gagaaccaga agttgggaag ataaagaagg taacaaaaaa tttgcaacag aaattgttgg
    16861 agataattta ataatgcttg ataaaagaaa tgagggtagc cattcagcta acgatataat
    16921 tgatgatggc tataacgata accaatcttt acccaatact gatgaaaact taccttttta
    16981 aaggttggta ttcttgtaaa attaataaca catacaacct aagtagttgt ataaatatta
    17041 ttcactaaag aaaatatttt gaacgatttt tcgatatttt ttaaacctac tcttgtaacc
    17101 ataattgact ctcaaggtac aaccatcttg tttgttttgt tacttttttt attggtatta
    17161 tcgtttattt taagtggtgc cgaagtggct tttttttcgt taaactataa agatattagt
    17221 gttttaaaaa caaagcagca gcccgtttat aaaagaatag taaccctttt ggaaaatcct
    17281 aaaacattat taggttctct tttaattgct aatacttttt caaatatttc tattattatt
    17341 ataagcaatg ttttattaga taacttaata cattttcatc caaattttga atgggttgag
    17401 tttttaataa aagtaacctt agtaaccttt atgcttgttt tatttagtga ggttatgcct
    17461 aaagtaatgg caacacaaaa taatgtgcgt tttgcaaaag atgtgggatg gctggtagaa
    17521 attgtatcgt atgtttttaa tagtttaagc aattggttag taaagtatag cgatttagtt
    17581 gaaaaaaaat tatctaataa aaacttaaac aacaatacca acgaagaact ttaccatgca
    17641 attgatatta cacatactga cagtaatgaa agtgaaaaaa atattttaaa aggaatttta
    17701 aagtttggca atactactgt taaacaggtt atgaaaacaa gattagatgt gcatggtatt
    17761 gattacaata tttcttttag cgaattactt caattaataa atgaactaca ttatagcagg
    17821 ttaccagttt ttaaagacga tttagatgaa atagctggaa ttattcatac aaaagatata
    17881 ctacaatact tagatgccga taataatttt aactggcact cccttttaag gcaaccttat
    17941 tttgttcatg aacaaaaaat gattgaagat ctcttaagtg aatttcagag caaacgaatt
    18001 cattttgcta tagtagtgga tgagtttgga ggtacaagtg gtattgttac tatggaagat
    18061 attatggaag aagtaatagg agaaattgcc gatgaatttg atgatgaaga tgcaggacaa
    18121 aagaaaatag atgacagaaa ttacattttt gaaggtaaaa ctatgataag agatgtttgc
    18181 aaattcatga atctttctat tgatactttt gatggtataa aaggagaaag tgattcttta
    18241 gcaggattaa ttttagaaat tgcaggcgaa ataccttatg ttacacaggt aattacttgt
    18301 ggcgattttg aatttaccat gttagaagta gctaaaaaca gaataaataa aattaaagtt
    18361 accattgtac cacacatggt agaataactt aacatttttt atatcaccca tacattatcc
    18421 taaaattact tattgcattt atttagtttt accttatgtt tgcaaccatc ttaaaaggtc
    18481 ctgtggccga gtg
//
LOCUS       DAOQPK010000017        18415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_1517_length_18415_cov_5.064815, whole genome shotgun sequence.
ACCESSION   DAOQPK010000017 DAOQPK010000000
VERSION     DAOQPK010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 18415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18415
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1517_length_18415_cov_5.064815"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            251..1282
                     /locus_tag="PLU36_01640"
     CDS             251..1282
                     /locus_tag="PLU36_01640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HMZ45482.1"
                     /translation="MKFLLFLCTSLVCCINNYAQDFTFSQFYEKPLLRNPSLAGVFDG
                     DLRVTGIYRNQWQSVTVPFNTSGASVEVKFPVGKGNDFLTVAMQMTNDVAGDIKLKRT
                     QILPVINFHKSLNDEYNSYLSLAFMAGNVSSRFDPSKLQMNDQFVNGSFNPGNPTMQS
                     FSKTSINYWDLGAGLTYSFDFGESSSLYFGASLFHLNKPKVSFYANNANSTTLHNKLS
                     LNAGLTTPTSDYNRLVFYADYFMQSGNKQFLGGILYGFDLVELYDSDEKISLYSGGFY
                     RWGDALIPVIKLNYYKLAVGLSYDINVSKLTTASQARGGFEISASYRTQLNIRNNGAE
                     KMRCVKFTL"
     gene            1545..2807
                     /locus_tag="PLU36_01645"
     CDS             1545..2807
                     /locus_tag="PLU36_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMZ45483.1"
                     /translation="MTIYRSTTKTILFSALLLLSVAFSNKAVAQDGKALFNANCASCH
                     KLDKDLTGPALMGVEERWGGSREKLIHWVKNSAAFLKTGDKHANELFNKWNKIPMPGY
                     DGILSDADINAIFDYIAKGAAPAPTDNKAAEVKPVEKSNNNLLFGILTLILAIVAFVL
                     INVNSNLKRLANEEQGLASSLPIPFWKNKTYITIIAVALFVTGGYLTTKGAIGLGRNT
                     EYQPVQPIFYSHKVHAGVNQINCLYCHGGAEGGKHANIPSVNVCMNCHKAIHEYTGPA
                     LVDGNGKEVNGTEELQKLFKYAGFDPKNANNWNPNDAKPIEWVQIHSLPDHVYFNHSQ
                     HVVAGKVQCQTCHGEIDKMDEVKQVNDLSMGWCINCHRETKVQFKDNGFYSIYEKYHN
                     ELKSGKLDSTKGITVEKIGGTECQKCHY"
     gene            2896..5982
                     /locus_tag="PLU36_01650"
     CDS             2896..5982
                     /locus_tag="PLU36_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HMZ45484.1"
                     /translation="MEKKYWQSFGELNNSEAFQKSSKDEFKEDLPFDEDKGLLEAKTP
                     RRDFLKYLGFSTAAAAVAASCEVPVKKAIPFANRPEEIIPGVANYYATTYVQDGDAVS
                     VVAKVRDGRPIKIEGNELCPITSGGTSARVQASVLDLYDGARLRFPANYTSSVKGKEL
                     TFDAADKAINEALATAGNIVLLTSTINSPSSLEVINQFLAKYPGSKQVTYDAVSYSGI
                     LLANEACYNKRAIPTYRFDAAKVIVSLGADFLATWISPIEFAKQYSKGKKINDASPEM
                     SKHIHFESVASLTGSNADERFLHRPSETYAIAVGLLSVLQGGEVSNIKDTKLIAGIKN
                     AAKALADKKGEALVVCGSNDVNTQIIINTINEILLAKDKTIDWSSTYNVRKGVDADFA
                     ALVEEMNTGTVGALIVIDANPVYSWLDKAKLKSGLDKVKLKISLSGKADETTQLCGYV
                     VPSHHYLESWGDAEVKVGHYSFIQPTIYPLFKTRQWQDSLMKWSAATTDYLAFLKSFW
                     ATKLTGTNSWDKALQDGVVMNSPLAVATTFNSSATNLALAAVQNPSSGIELQLYQKIA
                     IGAGQGTANPWLQEMPDPITRATWDNYLIVSPTMAKTLLKIDLSNNGQSDSYEVNPPK
                     KVVSIKANGITVELPALIIPGTHPDTVGIALGYGRDKKVGKTVVDVEGNSIGANAFPF
                     AKIVNGTVCFDINNVELKLTDKKYPVALTQTHNLYDTPHGKRTEVIKELTLAEYKHNT
                     KVVLEEREKELAAYGGVADFREGGSIYKPFDKPGIKWGMSIDLNSCNGCGACVVACNA
                     ENNIPVVGKPEVLRFHDMHWLRIDRYYSGDKENPDVVFQPMLCQHCDNAPCENVCPVA
                     ATNHSSEGLNQMTYNRCIGTRYCANNCPYKVRRFNWADYTGSDSFQNNQDELNNAVHY
                     MNDDLTRMVLNPDVTVRSRGVIEKCSFCVQRLQEGKLKAKVASRPLKDEDVHVACAQA
                     CPSDSIIFGNVNNPESEISKARKDSNRLFYSLEFLHVMPNINYLAKVRNKEEATKA"
     gene            6061..7500
                     /gene="nrfD"
                     /locus_tag="PLU36_01655"
     CDS             6061..7500
                     /gene="nrfD"
                     /locus_tag="PLU36_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HMZ45485.1"
                     /translation="MSLKYESQLREPLVYGNKTYHQVTEDIVRPIEAKPTKLWYIGFF
                     ISVSLLMFGVYSVYREVVYGVGEWNLNKTVGWGWDITNFVWWVGIGHAGTLISAILLL
                     FRQGWRTGVNRAAEAMTIFAVMCAGQFPVFHMGRVWMAFFVFPYPNTRGPLWVNFNSP
                     LLWDVFAISTYFTVSLLFWYSGLLPDFATVRDRAKTKLRKLLYGIASFGWTGSTKHWQ
                     RHESLSLVLAGLSTPLVLSVHTIVSFDFATSVIPGWHTTIFPPYFVAGAIFSGFAMVQ
                     SLMVVIRKVFGLKDYITIGHIEAMNKVIVLTGSIVGIAYLTELFMGWYSQSKYEGYTF
                     WYSRANLFSPYGWSYWGMMACNVLSPQIFWSRKMRRNVFVTFFMSVLVNVGMWFERFV
                     IIVTSIYRDYLPSSWSVYYSPSIWEIGFYLGTFGLFFTCFFLFAKYFPTIAIAEIKYV
                     LKTTGESYKDKMEGIENENVEDFAHKHAH"
     gene            7576..8163
                     /locus_tag="PLU36_01660"
     CDS             7576..8163
                     /locus_tag="PLU36_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HMZ45486.1"
                     /translation="MAKKKFVVGCFDNEDVLFSAVKKIRTAGYKIKDVYTPYPIHGLD
                     HALGMRETSLHTAGFVYGILGTITALSGISWIFVKDWPLDIGGKPHFALAAWIPIIFE
                     LTVLFSAVGMVLTFCYLCQMAPFVKKHHFHARATDDLFVMAIECTDVTNTNDTEAFLT
                     NNGAVEVKTEIAEDGWWFGRYDKEEMFFESKNAVA"
     gene            8185..8766
                     /locus_tag="PLU36_01665"
     CDS             8185..8766
                     /locus_tag="PLU36_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMZ45487.1"
                     /translation="MKKLAFITCIATTIIMVSCNSSVKRHPNDAYIPDMGYSRAYETY
                     ADHSNLKEKGIKYNNMPVAGTVARGEDFPFPLAHDAVGDTTNYHLSKTVPNPYDSLSE
                     TDLVEAERLYLVHCGICHGKKLDGNGPLWKDGSGPYPAAPKNLITLNMPDGQMFYSIT
                     YGKGQMGSYASQLSRKHRWQIVTYIKEKQKAGK"
     gene            8837..10063
                     /locus_tag="PLU36_01670"
     CDS             8837..10063
                     /locus_tag="PLU36_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:cytochrome C oxidoreductase"
                     /protein_id="HMZ45488.1"
                     /translation="MASIKQNFEIPSKMKTWSYVLMGIGLIALIAGFITKGMSHDHHE
                     KDVFLGTLMYNTIYWTLICNTAMFFICINTMAWAGWHVSFKRVSEAISTMVPIFGAIT
                     LIVLLYIAFSGSHIYHWTDTKAVAEDPILKGKSGFLNIKFFTIWTVLAIGLWSLLGWR
                     MRKLSAQADEAAMNKETGHGFVIKNTKVAATFLVWFGLTVGSTIPWLWMMSIDAHWFS
                     TMYSWYNFASTFVSGLALLALWIIYLKNKGYLEYTNQEHLHDVAKYMFAFSIFWAYLW
                     FSQYMLIWYSNQPEEVMYFKHRVQGAYKGIFFLNLIINFICPLLILMKKASKRNYTLV
                     TFMAVLIIFGHWIDYYQQVIGSISKDHVTLGWLDFGILSLFVGMMIFGVSKALASKPL
                     IPKYHPFLKESIIHHT"
     gene            10175..11212
                     /locus_tag="PLU36_01675"
     CDS             10175..11212
                     /locus_tag="PLU36_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HMZ45489.1"
                     /translation="MAKASEYVSILKGEEVTRKQTNKINAFLMLGFMIAGFIGVWYCN
                     EVLYHKTLLPFPAASDHGEKIDEMLWVTIWITGAVFIVTQFLLFWFAFKYQEKEGRKI
                     FYFVHSTKLEILWTSVPAIVLAVLIVVGLKHWFAITGDAPKNALEVEVVGKQFSWIFR
                     YKGKDNVFGKKYYKVIDEATNPLGLIWKDNKELGLKDDKKSHDDIVVQQTIYVVKNRP
                     VKLIIGSRDVVHDVGLPHFRMKMDAVPGTPTTMWFTPKYTTKEMKEITKNPNFVYEIS
                     CDQMCGNGHYSMKGTIEVVTQAEFNAWLAGLTPAYKSAMANNAIPNSKNAEPVMAAAA
                     SINNKLIAQSK"
     gene            11275..13098
                     /locus_tag="PLU36_01680"
     CDS             11275..13098
                     /locus_tag="PLU36_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HMZ45490.1"
                     /translation="MSNAAVLHSNIGTMHDDHSAHHEHHHQETFINKYVFSQDHKMIA
                     KQFLITGMVWALIGGFLSVLFRMQLGFPEESFPWLEDLLGKWWAAGGRITAQGYYALV
                     TTHGTVLVFFVLTAGLSGTFANFLIPLQIGARDMASPFMNMLSYWFFFAAGVIMLASI
                     FVQTGPFSGGWTAYPPLSALGDASPGSKTGMDMWIISMALFVVSSLLGGLNYIATILN
                     MRTKGMSMTRMPLTIWALLFTAILGVLSFPVLLSGFILLIFDRNFGTSFYLSDIFLAS
                     TNTALPNEGGSAILFQHLFWFLGHPEVYIIMLPAMGMVSEIISINSRKPIFGYLAMVG
                     SLFAIATLAFLVWAHHMFVTGLNPFLGSIFALLTLLIALPSSIKVFNWLTTLWRGNIR
                     LTPGMLFAIGFVSMFISGGLTGIWVGNATLDIHLHDTYFVIAHFHLVMGVASMFGMFA
                     GVYHWFPKMYGRHMNNTLAYIHFWITLIGAYLIFWPMHYQGLAGMPRRYYDYSIWESF
                     KQFSELNRFISIVVFITFIAQLLFLINFFYSIWKGRKATNLNPYGANTLEWTTPINPG
                     HGNWPGEIPEVHRWAYDYGKDGKDFITQTTPVGEEESTEHH"
     gene            13200..14102
                     /gene="cyoE"
                     /locus_tag="PLU36_01685"
     CDS             13200..14102
                     /gene="cyoE"
                     /locus_tag="PLU36_01685"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HMZ45491.1"
                     /translation="MTDKSLTYSTSFLSKLHDYKELTKFTLTFTVVFTCVISYLLAPS
                     IISYNLKMILLLFAAGLLITGSANAINQAVEKNSDALMKRTANRPVAAGRMSQKEAYT
                     FAFLTGAIGVFIMWYFFNTSSALLSAFSLFLYSFIYTPLKTKNSIAVLIGGLPGALPC
                     LIGWVAAEDSFSIGGWILFAIQFIWQFPHFWAIAWVAHKDYTAAGFKLLPSNEGPTKY
                     TAIQTIMYSVLMIPIGLLPYYFNITGSVSMYIILACNIAMVIQSIRLYNQMNVKAAKR
                     VMFSSYIYLPIVYLAMLADKIAIL"
     gene            14117..14698
                     /locus_tag="PLU36_01690"
     CDS             14117..14698
                     /locus_tag="PLU36_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HMZ45492.1"
                     /translation="MQIAMNNQPTTHRIHPHKFTLWMAIGSICMMFAGLTSAYIVKRN
                     QDNFLEFDLPIIFWLSTIVIILSSVTMHLALKAYKARKTNKFKMFITATAALGALFAA
                     MQAVGFLDLENHGIPLIGFKSNAAASFILVILGVHALHVLGGVVALVITFVKALFKKT
                     NQYNNIPVEVLSTYWHFVGILWIYLLVFLIWLR"
     gene            14793..15470
                     /locus_tag="PLU36_01695"
     CDS             14793..15470
                     /locus_tag="PLU36_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HMZ45493.1"
                     /translation="MSTVATAQPKLWAGGKSPFNVEYGKVMMWYFLMSDTFTFAAFLI
                     AYGTVRFSANSWPDPNEVFNTFPFAPEGVHTPLVFVSIMTFILIVSSVTMVLAVQAGH
                     HNDKKAVVKNLILTIIGGALFLGCQAWEWTHLYHEGAWWGKNPFLNADGTAPTVNFTN
                     FFFTITGFHGFHVFTGVIINIIMLIMTLNNTFEKRGHYLMIEKAGLYWHFVDLVWVFV
                     FTCFYLI"
     gene            15491..15922
                     /locus_tag="PLU36_01700"
     CDS             15491..15922
                     /locus_tag="PLU36_01700"
                     /inference="COORDINATES: protein motif:HMM:NF015582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HMZ45494.1"
                     /translation="MEYSTNSHGDYSAIKKEIWKVTLILSLLTIIELVFGFWIYAQPT
                     MGEGLKWTLKGLIIILMLSKAFYIVAYFMHLKHELRTFIMTIILPLISFVWIIFAFLY
                     DGNSFKDLRNGFDRHHKAQSKVKAVKPAATHNNTKKQEHGH"
     gene            16067..17380
                     /gene="der"
                     /locus_tag="PLU36_01705"
     CDS             16067..17380
                     /gene="der"
                     /locus_tag="PLU36_01705"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HMZ45495.1"
                     /translation="MSYTIAIVGRPNVGKSTFFNRLLEQRKAIVDDISGVTRDRQYGV
                     SEWAGKTFNVIDTGGFVPNSNDIFETEIRKQVKIAIDEADAIIFMVDVATGITDLDNS
                     MAHILRRSNKPVYLTVNKVDNHSRELDATEFYSLGFEKIYTLSSISGTGTGELLDAIT
                     EKIELTDIADNNTENELPKFAIIGQPNVGKSSLLNALIGQERTIVSNIAGTTRDTIHT
                     HYNLFNKEFILIDTAGIRKKSKEKDDLEFYSIIRAIKAMDEADICLLLLDAEKGITAQ
                     DVAIFSLAARKGKGIAILVNKWDLVEKETNTARDYEKKLKEKIAPFTDVPILFISVKD
                     KTRIFKAIEIALEVYNNKQQKISTSKLNEVMLKVVEANKAPVVRGHIVKIKFITQLPT
                     HVPSFAFFTNFPNDIKTPYKNFLENQLRAKFNFKGVPIRIFFRKK"
     gene            complement(17950..>18415)
                     /locus_tag="PLU36_01710"
     CDS             complement(17950..>18415)
                     /locus_tag="PLU36_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45496.1"
                     /translation="TFFYVTTLNMTDGLVVLSPSFLLSCPPLFVILPLSFLLSCLSRF
                     CHPASLVFVILPSLIFVILSPPVFVILSEAKDLKVKNNEIPLRLGMTNLFLCLYVQHD
                     RWVGCPEHLVFVTLHPLFCHPASLIFVILPPLFMSSYPLFYVILSVSEGSKG"
BASE COUNT         6218 a         2758 c         3412 g         6027 t
ORIGIN      
        1 cgatcctgca caaggttgga atggaacaat taatggcaaa ccagctagcc cagaggttta
       61 tgtttacact tgtgaagttg tttgcgaaaa taatgttgct tatacttaca agggtaatgt
      121 agcaattata aaataatggc tttaaataaa gaattatagg aaaatgaaaa atattattgt
      181 aattgaatgg aagaattaat tatatataaa tttataagat tgccaatggc agaagcacaa
      241 aaataaacac atgaaatttt tacttttttt atgtacctcg ttagtatgct gcataaataa
      301 ctatgcacaa gattttactt tttcgcaatt ttatgaaaaa ccattattgc gtaacccttc
      361 attggcaggt gtttttgatg gagacctaag agttacaggt atatatagaa accagtggca
      421 aagtgtaaca gtgcctttta acacttcagg tgcaagtgta gaagtaaagt ttcctgtagg
      481 taaaggcaac gattttttaa ccgttgcaat gcaaatgacc aatgatgttg caggagatat
      541 taaattaaaa agaacacaaa ttttaccggt aattaatttt cataaatcac taaatgatga
      601 gtacaacagt tatttatcat tagcatttat ggcaggcaat gtaagtagca ggttcgatcc
      661 aagtaaatta caaatgaacg atcagtttgt aaatggctct tttaatccgg gcaacccaac
      721 tatgcaaagc tttagcaaaa caagtattaa ttactgggat ttaggtgcag gcttaaccta
      781 tagttttgat tttggcgaaa gcagtagcct ttattttgga gcttctctct ttcatttaaa
      841 caaaccaaaa gtttccttct atgccaataa tgctaattct acaactcttc ataataaatt
      901 atccttaaat gctggtttaa caacacccac aagcgattac aatagattgg ttttttatgc
      961 agattatttt atgcaatctg gcaataaaca atttttaggt ggaatattat atggatttga
     1021 tttggtagaa ctctacgatt ctgatgaaaa gatatctctt tattctggag gattttatcg
     1081 ttggggcgat gctttaattc ctgttataaa actaaattat tataaattgg cagtaggttt
     1141 aagttacgat ataaatgtat ctaaattaac aacagcttct caggcacgtg gagggtttga
     1201 aatttctgca agctatagaa cacaattaaa cattagaaat aatggtgcag aaaaaatgcg
     1261 atgtgtaaaa tttactttgt aatacttctt ttgtttgtaa aaacatttta tccattaaat
     1321 actgaaacag taaaataatg gatgtttttt taacatcttt tttttacgaa aaaaggtaaa
     1381 aaaatatgac aatttgaagg ccgaaatttc aatattcgct gaaagtcaag ctacataagg
     1441 ataaattttt ttttttaaca ttgtgttttt gtcagattag tgtcatattt ttgcacctat
     1501 tcaagcattt tttatctcat attttagaat catagcttgt gaatatgacc atctacagaa
     1561 gtacaaccaa aacaatctta tttagtgcac ttttattatt aagtgttgct tttagtaata
     1621 aagctgttgc ccaagatggc aaagccttat ttaatgccaa ctgtgccagt tgtcataagt
     1681 tagataaaga ccttaccggt ccagcattaa tgggtgtaga agaacgctgg ggtggtagtc
     1741 gtgaaaaatt aatccattgg gttaaaaatt ctgcagcctt tttaaaaaca ggcgataagc
     1801 atgcaaatga actttttaac aaatggaata aaataccaat gcccggttac gatggtattt
     1861 taagtgatgc agatataaat gccatttttg attatatagc aaaaggtgca gcacctgcac
     1921 caacagataa caaagctgct gaagtaaaac ctgttgaaaa aagtaacaat aaccttcttt
     1981 ttggtattct aactctaatt cttgcaatag ttgcatttgt tttaataaat gtaaacagca
     2041 atttaaaaag acttgctaac gaagaacaag gtttagcttc tagcttacct attcctttct
     2101 ggaaaaataa aacttatatt acaataattg ctgtagcctt atttgtaact ggtggctatt
     2161 taacaacaaa gggtgcaatt ggtttaggta gaaatacaga gtaccaacca gtgcaaccta
     2221 ttttctactc tcacaaagta catgctggtg taaatcaaat aaactgtttg tattgtcatg
     2281 gtggtgccga gggaggtaaa catgcaaata ttccttctgt aaatgtttgt atgaattgcc
     2341 ataaagctat tcatgaatat actgggcctg cattggtgga tggtaacggc aaagaagtaa
     2401 acggaacaga agagctacaa aaattattca aatatgcagg atttgatcct aaaaatgcta
     2461 acaactggaa ccccaatgat gcaaaaccaa tagagtgggt tcaaatacat agtttaccag
     2521 atcatgttta ctttaatcac agccaacacg tagttgcagg caaagtgcaa tgccaaacct
     2581 gccatggaga aattgacaaa atggatgaag taaaacaagt gaatgattta agtatgggct
     2641 ggtgtataaa ctgccataga gaaactaaag tgcagtttaa agacaatggt ttttatagta
     2701 tttacgaaaa atatcataat gaactaaaga gtggtaagtt agatagtaca aaaggtataa
     2761 cagttgaaaa aattggcggt accgagtgtc aaaaatgtca ctattaataa caaacattaa
     2821 ttttttagtt aagtgattgt agagtaataa tatttttata aacattaaac tgttctcaaa
     2881 agaagataag ggcttatgga aaaaaagtat tggcaaagtt ttggcgaact gaataactct
     2941 gaggcttttc aaaaatcatc aaaagatgaa tttaaagaag accttccatt cgatgaagat
     3001 aaaggtttgc tagaagcaaa aacaccacgc cgtgatttct taaaatattt aggatttagt
     3061 acagcagcag ctgctgttgc tgcaagttgc gaagtgcctg taaaaaaggc aattcctttt
     3121 gcaaatcgtc cagaagaaat tataccaggt gttgcaaatt attatgcaac aacttatgtt
     3181 caagatggcg atgcagtaag cgttgtagct aaagttagag atggccgtcc tattaaaatt
     3241 gaaggaaatg aattgtgtcc tattaccagt ggtggtacaa gtgccagagt acaagccagc
     3301 gttttagatt tgtacgatgg tgcaagattg cgttttcctg caaattatac cagtagcgta
     3361 aaaggtaaag aactaacttt tgatgccgca gataaagcaa ttaatgaagc attggctaca
     3421 gctggaaata ttgttttatt aacaagcaca attaactctc cttcttcctt agaagtaatt
     3481 aaccaatttt tagcaaaata ccctggcagt aaacaagtaa cgtatgatgc tgtttcttat
     3541 tctggtattt tattagctaa tgaggcttgt tataataaaa gagctattcc aacctatagg
     3601 tttgatgcag caaaagtaat tgtaagctta ggtgccgatt ttttagccac ttggattagc
     3661 cctatagagt ttgcaaagca atactcaaaa ggtaaaaaaa taaatgatgc ttcaccagaa
     3721 atgagtaaac atattcattt tgaaagtgta gcaagcctta caggaagtaa tgcagatgaa
     3781 agatttttac accgccctag cgaaacctat gcaattgcag tgggcttact tagtgtgctt
     3841 caaggtggag aagtatccaa tattaaagac accaaattaa ttgctggcat aaagaatgct
     3901 gcaaaagcat tagcagataa aaaaggagaa gctttagtag tttgtggaag taatgatgta
     3961 aatacacaaa ttattattaa tacaattaac gaaattcttt tagctaaaga taaaacaata
     4021 gattggtcat ctacttacaa tgtaagaaaa ggggtagatg cagactttgc agctttagta
     4081 gaagaaatga atacaggaac agttggagct ttaatagtta tagatgctaa ccctgtatat
     4141 agttggttag ataaagcaaa attaaaatct ggtttagata aagtaaaatt aaaaatttct
     4201 ttaagtggca aagccgatga aacaacacaa ctttgtggtt atgttgtacc aagccaccac
     4261 tatttagaat cttggggaga tgctgaggtt aaagtgggtc attattcatt tatacagcca
     4321 actatttacc cactgttcaa aacacgccag tggcaagata gtttaatgaa atggagtgca
     4381 gcaactaccg attatttagc attcttaaaa agtttttggg ctactaaact aacaggtaca
     4441 aatagttggg ataaagcttt acaagatggt gttgtaatga atagtcctct tgctgttgct
     4501 acaactttta attcaagtgc tacaaactta gcattagctg cagtacaaaa tccatcatca
     4561 ggtattgaac tacaactata ccaaaaaatt gcaattggtg ctggtcaagg tacggcaaac
     4621 ccttggttac aagaaatgcc agaccctatc actagagcca cttgggataa ctaccttatt
     4681 gtttcgccaa caatggctaa aacattatta aaaatagatt tatctaataa tggtcagtca
     4741 gatagctatg aagttaaccc gcctaaaaag gtagtttcta taaaagctaa tggcattaca
     4801 gtagaattgc cagcattaat tattccaggt actcatccag ataccgttgg tatagcatta
     4861 ggttatggaa gagataaaaa agtgggtaaa actgtagtag atgtagaagg aaattcaata
     4921 ggtgcaaacg cttttccatt tgctaaaatt gtaaatggta cagtttgttt tgatattaat
     4981 aatgtagaat taaaacttac agataaaaaa tatcctgttg cacttactca aacacataac
     5041 ctttacgata cgccacatgg aaaacgtaca gaggtaatca aagaacttac tttagccgaa
     5101 tataaacaca ataccaaagt agttctagaa gaaagagaaa aagaattagc agcttatggt
     5161 ggtgtagcag attttagaga aggaggttct atttacaaac ctttcgataa accaggaatt
     5221 aaatggggca tgagcattga cctgaacagt tgtaatggct gtggtgcttg tgtagtagca
     5281 tgtaatgcag aaaacaatat acctgtagtt ggtaagccag aagttttgcg ttttcatgat
     5341 atgcattggt taagaataga tagatattat agcggagata aagaaaatcc agacgttgta
     5401 tttcagccaa tgttatgcca acactgcgat aatgcacctt gcgaaaacgt ttgtcctgtt
     5461 gcagctacta atcatagctc agaaggttta aaccaaatga catacaaccg ttgtattggt
     5521 acaagatact gtgctaacaa ctgtccttat aaagtacgta gatttaactg ggctgattat
     5581 acaggaagtg atagtttcca aaataaccaa gatgagttga acaatgcagt acactatatg
     5641 aacgatgatt taaccagaat ggttttaaat cctgatgtta ctgttcgttc tcgtggtgtt
     5701 attgaaaaat gctctttctg cgtacaacgt ttacaagaag gtaagttaaa agcaaaagta
     5761 gctagcagac cattaaaaga tgaagatgta catgtagctt gtgctcaagc ctgtccttct
     5821 gattctatca tttttgggaa tgtaaataat cctgagagtg aaataagtaa agcaagaaaa
     5881 gatagtaata gattatttta ttctttagaa tttttacatg ttatgcctaa tataaattat
     5941 ttggcaaaag ttagaaataa agaagaagct actaaagcat aggctttgta gaacagataa
     6001 atatttaaga acctaaacat caatttgttt taatacaaat tactaaataa ccaagtacag
     6061 atgagtttaa agtacgaatc gcaattaagg gaaccgttag tgtacggtaa taaaacctat
     6121 catcaggtaa ctgaagatat tgttcgccca atagaagcca aaccaactaa gttatggtat
     6181 attggttttt ttatttcagt atcgttgtta atgtttggtg tttacagtgt gtacagagaa
     6241 gttgtttatg gtgtgggaga gtggaacctg aacaaaacag taggatgggg ctgggatatt
     6301 accaacttcg tttggtgggt aggtataggt catgccggta cattaatttc agcaatctta
     6361 ttgttattcc gccaaggttg gagaactggt gttaaccgtg cagcagaggc aatgacaatt
     6421 tttgcggtta tgtgtgctgg tcagtttcct gtattccaca tgggacgtgt ttggatggca
     6481 ttctttgtat ttccttaccc aaatactcgt ggccctttat gggtaaactt taactcacca
     6541 cttttatggg atgtatttgc aatttcaact tactttacag tttccctttt attttggtat
     6601 agcggtttat taccagattt tgcaactgtt agagatagag ctaaaactaa attgcgtaaa
     6661 ctactttatg gtattgcatc ttttggttgg acaggttcaa ccaaacactg gcaacgccac
     6721 gaaagccttt ccttggtttt ggctggttta agtactccgt tagtattatc agtacacact
     6781 attgtatctt tcgactttgc aacctctgtt attccaggtt ggcatacaac aatatttcca
     6841 ccttatttcg ttgcaggggc aattttttct ggctttgcta tggtacaatc attaatggtt
     6901 gtaattagaa aagtgtttgg attaaaagat tatattacaa tcgggcatat tgaagcaatg
     6961 aataaagtaa ttgttttaac tgggtctatt gtaggtattg cctatttaac agaactattt
     7021 atgggttggt acagccaaag taaatatgaa ggatatactt tttggtatag ccgtgccaac
     7081 ttgtttagcc cttatggttg gagctattgg ggtatgatgg cttgtaatgt attaagccca
     7141 caaatcttct ggtctagaaa aatgagaaga aatgtgtttg taactttctt tatgagtgta
     7201 ttggtaaacg taggtatgtg gttcgaaaga tttgtaatca ttgtaacttc tatttataga
     7261 gattatttgc caagtagctg gtctgtttac tatagtcctt ctatttggga aattggcttc
     7321 tatttaggaa catttggttt gttttttacc tgcttcttcc tctttgctaa atatttccca
     7381 acaatagcaa tagcagaaat taagtatgta ttgaaaacaa caggagaaag ttataaagat
     7441 aaaatggaag gaattgaaaa tgaaaatgta gaagattttg cacataaaca tgcacattaa
     7501 ttttttacaa ttcattcaaa aaataaaaag ggtttaaaaa ttataaacaa tcatttatag
     7561 gtataaatgt aagttatggc aaaaaagaaa tttgtagtag gctgttttga taatgaagat
     7621 gttttattct ctgctgtaaa aaaaatacgt acagcaggat ataaaattaa agatgtttat
     7681 actccttatc caattcatgg cttagaccat gcattaggta tgagagaaac cagtttacat
     7741 actgctggtt ttgtgtatgg catattaggt actattactg ctttaagtgg cattagctgg
     7801 atttttgtga aagactggcc attagatatt ggtggtaaac cacattttgc attagcagca
     7861 tggataccaa ttatttttga acttactgta ttattttctg ctgttggtat ggttcttact
     7921 ttttgctatt tatgccaaat ggcaccattt gtaaaaaaac atcactttca tgccagagct
     7981 accgacgatt tatttgtaat ggcaattgaa tgtaccgatg taaccaatac caacgataca
     8041 gaagcatttt taacaaacaa tggagctgta gaagttaaaa cagaaatagc agaagatggt
     8101 tggtggtttg gtagatatga taaagaggaa atgtttttcg aatcaaaaaa cgcagttgca
     8161 taatttatta gataaagtaa acaaatgaag aaattagcat ttataacatg tatagcaacc
     8221 accattataa tggtaagttg caatagcagt gtaaagcgtc atcctaatga tgcttatatt
     8281 ccagacatgg gttatagcag ggcttatgaa acctatgcag accatagcaa tttaaaagag
     8341 aaaggcatta aatataataa tatgccagtg gctggaacag ttgcaagagg cgaagatttt
     8401 ccttttcctt tagctcatga tgctgtagga gatactacta attatcattt atccaaaaca
     8461 gttcctaacc catacgattc tttatctgaa actgatttag tggaagccga aagactttat
     8521 ttagtgcatt gtggtatttg ccatggaaaa aaattagatg gcaatggccc actttggaaa
     8581 gatggttcag gaccttaccc tgcggcacct aaaaatttaa ttactttaaa tatgcccgat
     8641 ggacaaatgt tttacagtat tacctatggt aaaggccaaa tgggtagtta tgcttctcaa
     8701 ctaagccgta aacaccgttg gcaaattgtt acctatatta aagaaaaaca aaaagctggt
     8761 aaataattgt aactattata tttaagtatt ttataaataa acagagatta agctatacta
     8821 gaaaaaacta attaaaatgg catctattaa acaaaatttc gagattcctt caaaaatgaa
     8881 aacatggtct tatgtactta tgggcatagg attaatagca ttaattgcag gatttataac
     8941 taaaggcatg agccatgacc accacgaaaa agatgttttt cttggtacat taatgtacaa
     9001 taccatttat tggactttaa tttgcaatac tgccatgttt tttatttgta ttaatacaat
     9061 ggcatgggct ggttggcacg tatcttttaa aagagtttca gaagctattt ctacaatggt
     9121 tccaatattt ggtgctatta ctttaatagt tttattatat atagcatttt ctggtagcca
     9181 tatttaccac tggacagata caaaagcagt tgccgaagat cctatattga aaggtaaatc
     9241 aggcttctta aacataaaat tctttaccat ttggacagtt ttagcaattg gcttatggag
     9301 ccttttaggt tggagaatga gaaaattaag tgcccaagcc gatgaagctg caatgaataa
     9361 agaaacagga catggatttg taattaaaaa tacaaaagta gctgcaacct ttttagtttg
     9421 gtttggctta actgttggct ctactattcc atggctttgg atgatgagta tagatgcaca
     9481 ttggtttagt acaatgtata gttggtataa ttttgccagc acatttgtta gcggattggc
     9541 tttattagcc ctttggataa tttacttaaa aaataaaggt tatttagaat ataccaacca
     9601 agagcatttg catgatgtgg caaaatacat gtttgctttc tctatttttt gggcttattt
     9661 atggttctct cagtacatgc taatatggta ctctaaccaa ccagaagaag tgatgtattt
     9721 taaacataga gttcaaggtg catacaaagg aatcttcttc ttaaacctta ttataaactt
     9781 tatttgtcca ttattaatat taatgaaaaa agcttctaaa agaaactaca ctttagtaac
     9841 ttttatggct gttttaatta ttttcggaca ttggatagat tattaccaac aagtaattgg
     9901 tagcattagc aaagaccatg taaccttagg ttggttagat tttggaattt taagcttatt
     9961 tgtaggtatg atgatatttg gtgtaagcaa ggcattagca agtaaaccac ttattcctaa
    10021 ataccatcct ttcttaaaag aaagcattat tcaccatact taacagataa gtatctaaaa
    10081 cctgcatttt aaaaattaag aaaacagtta tataaccatg acaatgtttt taatcataac
    10141 agtagttgtt ctagtattct tagtcatttt tcaagtggct aaagcaagcg aatatgtatc
    10201 tatactaaaa ggcgaagaag taaccagaaa acaaactaat aaaataaatg cctttttaat
    10261 gcttggcttt atgattgctg gctttattgg cgtttggtat tgtaatgaag ttttgtacca
    10321 taaaacctta ttaccttttc ctgctgctag cgaccatggc gaaaaaattg acgaaatgct
    10381 ttgggttact atttggatta ctggagcagt ttttattgtt acgcagtttt tacttttttg
    10441 gtttgcattt aaatatcaag aaaaagaagg aagaaaaata ttttactttg tacatagcac
    10501 caaattagaa attctttgga catccgttcc tgcaattgtt ttagcagttt taattgttgt
    10561 tggcttaaaa cattggtttg ctattactgg cgatgcacct aaaaatgctt tagaagtaga
    10621 agttgtaggc aaacaattta gttggatttt tagatacaaa ggaaaagaca atgtatttgg
    10681 taaaaaatat tataaagtta ttgatgaagc aaccaatcca ttgggcctaa tttggaaaga
    10741 caataaagaa ttaggtttaa aagatgataa aaaatcgcat gatgatattg tggttcaaca
    10801 aacaatttat gtagttaaaa atagacctgt aaaactaatt attggtagta gagatgtagt
    10861 gcatgatgtt ggcttaccac attttagaat gaaaatggat gcagtgccag gcacgccaac
    10921 tactatgtgg tttactccta aatataccac aaaggaaatg aaggaaatta ccaaaaatcc
    10981 aaactttgtg tatgaaataa gttgtgatca aatgtgtggt aatggacact actctatgaa
    11041 aggaaccatt gaagtagtaa cccaagcaga gtttaatgca tggttagcag gcctaacacc
    11101 agcgtacaaa agtgcaatgg caaacaatgc aataccaaat tcaaaaaatg cagaaccagt
    11161 aatggctgct gcagcatcta taaacaataa actaattgct caaagtaagt agcaattttg
    11221 caaaagtttt tttaataatt ttttagaaca ctatacaaga aataagtatt tattatgagt
    11281 aacgcagcag ttctacattc aaatattggt accatgcatg acgatcattc tgctcatcat
    11341 gaacaccacc accaagaaac ttttataaac aaatatgtat ttagccaaga ccataaaatg
    11401 attgctaaac agtttttaat aactggtatg gtatgggctt taattggtgg ctttttaagt
    11461 gttttattta gaatgcaatt aggttttcca gaagagtctt ttccttggtt agaagattta
    11521 ttaggcaaat ggtgggctgc aggtggtaga attactgctc aagggtatta tgccttagtt
    11581 actacacacg gtactgtact agttttcttt gtattaactg ctggcttaag tggtactttt
    11641 gcaaacttct taattcctct gcaaattggt gcaagagata tggcttcgcc ttttatgaat
    11701 atgcttagct attggttttt ctttgcagct ggggttatta tgttagcttc tatttttgtt
    11761 caaacaggtc cttttagtgg tggttggaca gcctatcctc ctcttagtgc tttaggagat
    11821 gcttctccag gttctaaaac tggtatggat atgtggatta tttctatggc tttgtttgtt
    11881 gtatcctctt tattaggggg cttaaattat attgctacca ttttaaacat gcgtaccaaa
    11941 ggtatgagca tgactagaat gccattaaca atttgggctt tattatttac agctattcta
    12001 ggcgttttat ctttccctgt gttattatct ggttttattc ttttaatttt tgatagaaat
    12061 tttggaacaa gtttttattt atctgatatc ttcttagcat ctaccaatac agccttacca
    12121 aacgaaggtg gtagtgccat tttattccaa catttattct ggttcttagg ccacccagag
    12181 gtttatatta ttatgcttcc tgcaatgggt atggttagtg aaatcatcag tataaattct
    12241 cgtaagccta tttttggtta tttagcaatg gtgggtagtt tatttgcaat tgctacactg
    12301 gcatttttag tttgggcaca ccacatgttt gttacaggtt taaatccttt cttaggttca
    12361 atttttgctt tacttacttt attaattgcc ctacctagtt caattaaagt atttaactgg
    12421 ttaactacat tatggcgtgg taatattagg ttaacgcctg gtatgctgtt tgccattggc
    12481 tttgttagta tgtttatatc tggtgggtta actggtattt gggtaggtaa tgcaacttta
    12541 gatattcatt tacacgatac ttactttgtt attgctcact tccacttagt aatgggcgta
    12601 gctagtatgt ttggaatgtt tgcaggtgta tatcattggt ttcctaaaat gtatggaaga
    12661 cacatgaaca atactttagc gtatatacac ttttggataa cattaattgg tgcttaccta
    12721 attttctggc caatgcacta ccaaggttta gctggtatgc ctcgtagata ttatgattac
    12781 agcatttggg aaagctttaa acaattcagc gagttaaaca ggtttattag tattgtggtg
    12841 tttatcactt tcatagctca attattattt ttaattaact ttttctattc catttggaaa
    12901 ggaagaaaag ctactaactt aaacccttat ggtgccaata ctttagagtg gacaacacca
    12961 attaaccctg gtcatggtaa ctggcctggc gaaattcctg aagtacaccg ttgggcttat
    13021 gattatggta aagatggtaa agactttatt acccaaacca ctcctgttgg agaggaagaa
    13081 agtacagagc accactaaaa tttatggtat agtaacatct aaattataaa tgatttatgt
    13141 agaaaatgcc ctgaactttt cggggtattt ttttgaattt ttttattact taattcttaa
    13201 tgacagataa aagcctaaca tattctactt cttttttatc taagctacac gattataaag
    13261 agctcactaa atttacgcta acttttaccg tagtttttac ctgtgttatc agttatttat
    13321 tagccccaag cattattagt tacaacctaa aaatgatttt gttacttttt gctgctgggt
    13381 tattaataac aggtagtgca aatgccatca atcaggctgt agaaaaaaac tctgatgctt
    13441 tgatgaaaag aactgcaaat aggcctgttg ctgctggcag aatgagccaa aaagaagcct
    13501 atacttttgc ttttttaaca ggtgcaatag gtgtgtttat tatgtggtat tttttcaata
    13561 cctcttctgc cttactttct gcctttagtt tatttcttta ttcatttatt tatacgccat
    13621 taaaaactaa aaattctatt gcagttttaa ttggtggttt accaggtgcc ttgccttgtt
    13681 taattggttg ggttgctgcc gaagatagtt ttagcattgg cggatggata ctgtttgcca
    13741 tacaatttat ttggcaattt ccacattttt gggctattgc atgggtggct cataaagatt
    13801 atacggctgc tggttttaaa cttttaccaa gcaacgaagg accaactaaa tatactgcca
    13861 tacaaacaat tatgtatagc gttttaatga ttcctatagg gcttttacca tattacttta
    13921 atataacagg ttccgtgagt atgtatatta ttttagcttg taatattgca atggttatac
    13981 aaagtataag attgtataac caaatgaatg taaaagcagc aaaaagagta atgtttagta
    14041 gttatattta tttaccaatt gtatatttag caatgctagc cgataaaata gctatacttt
    14101 aaattttaat tgtattatgc aaatagccat gaacaatcaa ccaactacac atagaattca
    14161 tccccacaaa tttaccttat ggatggccat tggtagcatt tgtatgatgt ttgcaggctt
    14221 aacgagtgcc tatattgtta aacgcaatca agataatttt ttagaatttg atttacccat
    14281 tattttttgg ttaagtacta ttgtaattat tttaagcagt gtaactatgc accttgcttt
    14341 aaaagcctat aaagcaagaa aaacaaacaa gtttaaaatg tttattacgg caactgctgc
    14401 tttaggtgct ttatttgctg ctatgcaggc agtaggtttt ctagacttag aaaatcatgg
    14461 tattccgtta ataggcttta aaagcaatgc tgcagcatct tttattttgg taattttagg
    14521 agtacatgca ttgcatgtat taggtggtgt ggtagcatta gtaataacat ttgtaaaagc
    14581 actatttaaa aaaacaaatc agtataacaa cataccggta gaagtattaa gtacttactg
    14641 gcattttgta ggaattttat ggatttattt gcttgtattt ttaatttggc tacgctaaaa
    14701 taaaatagtt gtaaaatcgt taaaaaaaat agcaattaaa ttatattttt aaagtttaag
    14761 ctaatatttt tgtaacacaa acagaccaaa atatgtcaac tgtagcaact gcacaaccta
    14821 aattatgggc aggtggtaaa agccccttta atgtagagta tggtaaagtt atgatgtggt
    14881 attttttaat gagcgatact tttacatttg ctgcctttct aattgcttac ggaacggtaa
    14941 gattttctgc caatagctgg cccgatccta atgaagtttt taatactttc ccttttgcac
    15001 cagaaggggt acatacacca ttggtatttg taagtatcat gacttttatt ttaattgtta
    15061 gctctgtaac aatggtatta gctgtacaag ctggtcacca taacgataaa aaagcagtag
    15121 ttaaaaacct tattctaaca attattgggg gtgctttatt cttaggctgt caagcttggg
    15181 agtggacgca tttataccac gaaggtgctt ggtggggtaa aaaccctttt cttaatgcag
    15241 atggtactgc acctactgta aactttacca acttcttctt tactattaca ggctttcacg
    15301 gctttcacgt atttacagga gtaatcatta atataatcat gttaataatg accctcaata
    15361 atactttcga aaaaagaggc cattacctaa tgattgaaaa agctggctta tactggcact
    15421 ttgtagattt agtttgggta tttgtattta cttgttttta cttaatttaa aacttaataa
    15481 atttttataa atggaatatt caactaactc tcatggcgat tattctgcca taaaaaaaga
    15541 aatttggaaa gtaaccctta tcctttcttt acttactatt attgaacttg tttttggatt
    15601 ttggatttat gcccaaccta ctatgggcga aggtttaaaa tggacattaa aaggcttaat
    15661 cattatttta atgctctcta aagcatttta cattgtagct tatttcatgc acttaaaaca
    15721 tgagttaaga acttttatta tgaccattat tttaccttta atttcttttg tgtggattat
    15781 tttcgccttc ttatacgatg gcaattcttt taaagattta agaaacggtt ttgaccgcca
    15841 ccataaagca caaagcaaag taaaagctgt taagcccgct gctacgcaca ataatacaaa
    15901 aaagcaagag catggccact aaacagccat acaactaatt tgtaaaccat cggtacaacc
    15961 gatgtttttt ttattctttt cgccttcctg tttttttaaa ctaatattgc agttttaagt
    16021 ttgtatatac cgcttttaat aagcccaaag caataaatat tttagaatga gttataccat
    16081 tgcaattgta ggaagaccta atgtaggcaa aagcactttt tttaataggc tgttagagca
    16141 acgtaaagct attgtagatg atattagtgg tgtaaccaga gatagacaat atggtgtaag
    16201 cgaatgggca ggtaaaactt ttaatgttat tgatacaggt gggtttgtgc ctaacagcaa
    16261 tgatattttt gaaaccgaaa taagaaaaca ggtaaaaata gctatagacg aagctgatgc
    16321 tattattttt atggtagatg tagcaactgg tattacagat ttagataact ctatggctca
    16381 tattttaaga cgttctaata aaccagttta tcttactgta aacaaagtag ataaccatag
    16441 cagagaatta gatgctaccg aattttatag tttagggttc gaaaaaattt atactcttag
    16501 cagcattagt ggtacaggca ctggcgaatt attagatgca attacagaaa aaattgaact
    16561 aaccgacata gcagataata atacagaaaa tgaactgcca aaatttgcca ttattggtca
    16621 accaaatgtt ggcaaatcat ctttactaaa tgcactaatt gggcaggaga gaaccattgt
    16681 tagcaatatt gcaggcacta ccagagatac tattcataca cattataact tatttaataa
    16741 agaatttatt ttaatagaca ctgcaggtat tagaaagaaa agtaaagaaa aagacgacct
    16801 ggaattttac tctatcatta gagctattaa agcaatggac gaagcagata tttgtttatt
    16861 gcttttagat gcagaaaaag gaataacggc acaagatgta gccattttta gcctggctgc
    16921 ccgtaaaggt aaaggcatag ctattttagt aaataaatgg gatttggtag aaaaagaaac
    16981 taatactgca agagattatg aaaaaaaatt aaaagaaaaa atagccccgt ttaccgatgt
    17041 gcctatttta tttatatctg taaaagataa aacccgaatt tttaaagcca tagaaattgc
    17101 tttagaagtg tataacaata aacaacaaaa aattagcact tctaaactaa acgaggtaat
    17161 gctaaaagtg gtagaggcca ataaagcacc agtggtacgg gggcatattg taaaaattaa
    17221 gtttataaca caattaccaa cccatgtacc ttcctttgca ttttttacta actttccaaa
    17281 cgatattaaa accccgtata aaaatttttt agaaaaccag cttagggcaa aatttaattt
    17341 taaaggagta ccaattagaa ttttctttag aaagaaataa tagccatgta aaccttttta
    17401 attgtgaata attaattggg taattgtgta tttttttata taattctttt ttattattaa
    17461 ctcatttaag ctatctttat cgtttattat ttttgcaaag caacttttat ttttaaaaat
    17521 aaaaagctac tgctaacaaa actggtaaaa cccttaaaac aataacaaca accaaccaat
    17581 gccaacaagg catggtatta atattaattt atagtgaaca ttttttaagt aggcttacct
    17641 gcttataggg tgctttactt ttgtttaaac tgtggcatta tgaatgaaat gaaaaaaaag
    17701 aattgtcatg ttgaacgaag tgacataaaa aaaggtttgt cattccgagc ctgagaggaa
    17761 tctcattgtt cttaaccttt agatccttcg cttacgctca ggatgacaaa aagagggggc
    17821 aggatgacaa aaaagagggt gtaggataac aaaaatgagg gggcagtaca accaaacctt
    17881 gtcatgttga acgtagtgaa gaatctttac ttgtctgcca ttccgagcct gagaggaatc
    17941 tcattgttct taacctttag atccttcgct tacgctcagg atgacataaa agagggggta
    18001 ggatgacata aaaagtgggg gcaggataac aaaaatgaga gaggcaggat gacaaaaaag
    18061 agggtgtagg gtaacaaaaa cgaggtgctc aggacaacca acccatctgt catgttgaac
    18121 gtagagacat aaaaaaaggt ttgtcattcc gagcctgaga ggaatctcat tgttcttaac
    18181 ctttagatcc ttcgcttcgc tcaggatgac aaaaacagga gggctcagga taacaaaaat
    18241 gagagagggc aggatgacaa aaacgagaga ggcaggatga caaaaacgag agaggcagga
    18301 taacaaaaac gagagaggca ggatgacaaa aagaggaggg caggataaca aaaatgaggg
    18361 gctcaggaca accaacccat ctgtcatgtt gagcgtagtg acataaaaaa aggtt
//
LOCUS       DAOQPK010000018        18313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_1528_length_18313_cov_4.215686, whole genome shotgun sequence.
ACCESSION   DAOQPK010000018 DAOQPK010000000
VERSION     DAOQPK010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 18313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18313
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1528_length_18313_cov_4.215686"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PLU36_01715"
     CDS             complement(<1..609)
                     /locus_tag="PLU36_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587618.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HMZ45497.1"
                     /translation="MSNENEIKHHTDSGQCPYHQVAKNALPTGSNKEIKKDWWPNQLN
                     LQVLRQNSSLSNPMDKDFDYAQAFNQLDYFALKADLAKLMTDSQEWWPADFGNYGPFF
                     IRMAWHSAGTYRIGDGRGGAGTGQQRFSPLNSWPDNGNLDKARRLLWPIKQKYGSKIS
                     WADLMILTGNVALETMGFKTLGFAGGRADVWEPESDIYWGSET"
     gene            721..1671
                     /locus_tag="PLU36_01720"
     CDS             721..1671
                     /locus_tag="PLU36_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HMZ45498.1"
                     /translation="MTLIQLEYIIAVSNLKHFAKAANFCNVTQPTLSMQIQKLEEEFE
                     VKIFDRSKQPVIPTILGEKIIEQAKRIIAEKENLSQLLIQTKGETAGELKIGIIPTLA
                     PYLLPLFLNKFIKKYPKVKLKITELNTNSIIKKLKENTIDIGILVTPLNEKVISETPL
                     FYEELMAYISKRNKEFNKTYMLTKDIDIKKLWLLEEGHCFRTQVLNLCELRKNMNGES
                     QFEYAAGSFETLRKMVDVTDGLTILPELATEYFSASEMQRIRHFKAPAPVREISIVLH
                     RNYAKQQLVTILKDEILNAIPEKIKKNKKTFIVPFESKES"
     gene            1697..2206
                     /locus_tag="PLU36_01725"
     CDS             1697..2206
                     /locus_tag="PLU36_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45499.1"
                     /translation="MKKILILLFVMAFNYNSFSQGIATESAKQFYNKIFEYDYVEKKP
                     IFVAGRDSLRNFYLSHFNAFDSIVTRAIIKGDTAKYLRIHFEYIIDENGVPYNSQFKY
                     IGSTRYGSSSGDKKLKYFNDLNSYFKEATKTMIHKMPNWRPALQNNIRVKCNVEDYFQ
                     IWLGINPEK"
     gene            2237..4021
                     /locus_tag="PLU36_01730"
     CDS             2237..4021
                     /locus_tag="PLU36_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ45500.1"
                     /translation="MKILIRYIKPYKWLIALAFLLATINQIFSLLDPLIVGKVIDLFA
                     NHPKHFDTNKTLLRTENEFLFGSSTFHGLIFFILLLIGTAMVSRVAKAFQDYTVNVII
                     QKFGAGIFTDGLKHSMQLPFQEFEDQRSGETLSILTKVRTDTEKFISYFINVFLSIII
                     SIVFVSIYAFKLHWSIVPVYMGGSILIGFTTSLLSKKIKNIQKNIVKETTVLAGSTTE
                     SLRNIELVKSLGLTNEEVKRLNNNTYKILGLELKKVKSLRTLSFIQGTMINFLRQVIT
                     FTLLWLIYKDVLTTGQFISLQFYSFFIFGPMQEIGNIIISYREAQASLLNFDELMKKK
                     PEPKPTSPLHLGIIKEVSFEHISFKHTSAKYKALDDISFKVNEGETVAFVGPSGSGKS
                     TLVKLLVGLYTPQEGNIKYNNLYSKEIDFDELRTQIGFVTQDTNLFAGTIKENLLFVN
                     SSATNDELNDVLEKASCQNLLERADNGINTVIGEGGLKLSGGEKQRISIARALLRKPH
                     LLIFDEATSALDSITEEEITNTIKQISLHKKQVTIMIAHRLSTIMHADRIFVLEKGKI
                     VETGNHQELLNEKGLYFAMWRQQIGERK"
     gene            complement(4032..5138)
                     /locus_tag="PLU36_01735"
     CDS             complement(4032..5138)
                     /locus_tag="PLU36_01735"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HMZ45501.1"
                     /translation="MSAILSIFNDFFIHSFWMKVLKLYNKKVETKFYFKFCFFILFGL
                     GIYQNSNAQVATVDKIKMYAPYRFNLLTEQNFFDSLSQNTPSHLRNYKRIRLVSAVNI
                     VGYGGAMIGLYNAWYKNYPQSNFHFFNDNEEWLQVDKAGHAYSAYVESKASMELWRWA
                     GLPRKQRIWLGGLSGATYQTAIEFLDAYSVGWGWSWGDFIANCYGSGLLIAQELLWDE
                     QRVDLKFSFHKVNYKDPFLHNRADKLFGKSFLNRMLKDYNGQTYWLSANIKSFFPKSN
                     VPSWLNVAVGYGADGMFDGDDNIWKDENNNVIDRSDIPRYRQWYIAPDINLTKIKTKS
                     KFLKVALFFLNSFKFPAPSIGFSKKGVEFNWLHF"
     gene            5210..6934
                     /locus_tag="PLU36_01740"
     CDS             5210..6934
                     /locus_tag="PLU36_01740"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HMZ45502.1"
                     /translation="MDNSISVKVNEWLGGNYDETTKQIIKNLQAENELELTESFYKNL
                     EFGTGGLRGIMGVGTNRMNKYTVGMATQGFANYLKKVYGQEEIKIVIGHDSRNNSRFF
                     AETTANVFAANGCKVFLFEALRPTPELSFAIRHLGCKAGVVCTASHNPKEYNGYKAYW
                     NDGSQLVPPHDKNVIKEVEAIANVDEVKWTGGQANITLLGKEMDEAYIGMVKALSIYP
                     DVIAKQHNLKIVYTPIHGTGITIVPETLKQFGFSNVTIVEEQATPDGNFPTVVYPNPE
                     ETEAMSIGLKKAIEIDADILCGTDPDSDRVGIGVKNHKGEWVLMNGNQTLVLAFAYLI
                     EARKLKGLAKPNDMVISTIVSTDMAHNVAQQNNISSYGVLTGFKWIAELIREKEKDEN
                     YIIGGEESFGLMIGNGTRDKDAVSAVALLCEMAAYEKEHGRSLFDKLIELYIKYGFYY
                     ESLISITKKGMNGQAEIAEMMEGYRTNPPKVINGSKVVEILDYEKQIGKDFESGDSWK
                     IELPKSNVLQFITEDGSKISARPSGTEPKIKFYFSVNTKLNSKNEFDEKFELLQNKIK
                     GIIASLNL"
     gene            complement(7006..7455)
                     /locus_tag="PLU36_01745"
     CDS             complement(7006..7455)
                     /locus_tag="PLU36_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMZ45503.1"
                     /translation="MQQAKKGDTVKIHYHGRLTDGTTFDSSEGREPLQFELGSGMVIP
                     GFDNGVTGMSVGEKKTINIPAAEAYGESNPDMIMDFPKAQFPPDLTPEVGMQLTMHNG
                     AGQQFNVTIIELKEESVILDANHHLAGKELIFDLELVDIKAKSLIIT"
     gene            7756..7980
                     /locus_tag="PLU36_01750"
     CDS             7756..7980
                     /locus_tag="PLU36_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HMZ45504.1"
                     /translation="MFWTLELASYLEEAPWPATKDELIDYSIRSGAPIEVVENLQELD
                     DEGTEVYESIEDIWPDYPSQDDFMFNEDEY"
     gene            complement(8008..9528)
                     /locus_tag="PLU36_01755"
     CDS             complement(8008..9528)
                     /locus_tag="PLU36_01755"
                     /inference="COORDINATES: protein motif:HMM:NF017390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HMZ45505.1"
                     /translation="MQPIATSFLLQSLGYTLINSLWQVAIIWLLYILFTNFLKLPSKH
                     KYLIAVVAQIIGFCSFIYTFCYNFFSTSSNLKNGLFLQSNNFNSFFIPLILKVEKALP
                     FLSVVYLLVTIILLVKVIIGFNHTNNLRINKLHKVNAEWRVFVNEMAIALGLNKEVKI
                     YLSELVNTPLTIGYFKPIILLPLASINNLSTYQIEAVILHELAHIKRFDFLVNILLLI
                     VEIVLFFNPFSRLLSEHIKQERENSCDDWVLQFKYCPNLYANALLQIAMSNLRPYQFG
                     MAAISNKNELLKRVNRITNSKSAKNNYSALRFGFVFMLFALLSFTFFFSGNIQNESIN
                     KATNRLLSNTIKNEVKTLSHTGLAQNKSSNNQQEQYILFEKLVKEPADIESISTNDNK
                     RKLSTNSEVGKPFNSKKNSPKNSKPKINDTELEEEDFEIPESSMPFSYAFNTLNDSSY
                     TVTATDIEQENNISKLIIRKKGLTEKELEKIIPKRYDIVISNEGKPDKKIILEVWQ"
     gene            complement(9537..9920)
                     /locus_tag="PLU36_01760"
     CDS             complement(9537..9920)
                     /locus_tag="PLU36_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HMZ45506.1"
                     /translation="MFNKQKIKPTESELEILRILWDKKSASVREVHEILSLHKESGYT
                     TTLKLLQIMFEKSLVIRDDTSKVHIYTPNVSKESTQKQFMHKMVETLFGGSSVQLAMQ
                     ALGNSKPSKDELEEIEKILNRLKNK"
     gene            complement(10048..11418)
                     /locus_tag="PLU36_01765"
     CDS             complement(10048..11418)
                     /locus_tag="PLU36_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ45507.1"
                     /translation="MKKIILLFVVGLITTTSFAQNQKADYKKRPTLSVNFFMKDFLTP
                     SRIGTSSLSSVIGNKQWAQFADMVPGLGVSYIKGISNHMDFASNLGGSFIDYPFLDAP
                     KLGKEKFLLELDATLRLKLLTDKYIVVPYVSGGVGATALGGSHFGAYIPVSTGLQFNL
                     GQSEAFLFTDVSYRYPVTQKTGNYHLNYSIGLGAPLCDKPAAKPVEPPMPPKKVEPKD
                     TDKDGIVDSLDKCPTVYGIAKYQGCPIPDTDKDGINDEEDKCPTVYGVAKYQGCPIPD
                     TDKDGINDEEDKCPTVAGLARYQGCPIPDTDKDGINDEEDKCPTEAGPAHNNGCPVLT
                     EEKKQKVQLYAKNIYFATGKSALLTKSYKSLDTIVALLKEKGNETLGLNIEGYTDNVG
                     KPASNLKLSQSRANAVMGYFKKKGIAENRLSAKGFGQESPVADNKTAQGRALNRRVEL
                     KLVEKQ"
     gene            11506..12228
                     /locus_tag="PLU36_01770"
     CDS             11506..12228
                     /locus_tag="PLU36_01770"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HMZ45508.1"
                     /translation="MNKKYYIFFLLCFIISCKSNKQIFSKNEYGLIVLTTPKQYFSTV
                     SLDSSKLLKRLNGFIPNIVFDWRYATTNNFTKQILYTNPDAYLRIEAANNLKNVAIEL
                     EKIGLGIKVFDAYRPYSVTKKMWEVVPDDRYASDPAKGSGHNRGIAIDLTLYNLETGT
                     ELEMPTSYDNFTENAHANYQQLSQIVLKNRTILINTMQMFGFKVLDTEWWHFYLPNSS
                     KYELMDYDFKTMSKMCNVNTHK"
     gene            complement(12285..13817)
                     /locus_tag="PLU36_01775"
     CDS             complement(12285..13817)
                     /locus_tag="PLU36_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HMZ45509.1"
                     /translation="MNSNLKNLFTVVIVSAATAVLSVVGYDKYMQNKYAGTQEEGKLP
                     VNYARFFDNTNAPIGADFSLAATSATPAVVHIKTKTKSKQISNNAPRSRNPFSDMFGG
                     DMFDEFFNGPRMQVIPEQQASGSGVIVSKDGYIVTNNHVVDGADEIKVTLTDKKTYTA
                     SIIGTDPSTDLAVIKIEGNNFPYLIYGNSDEVKLGQWVLAIGYPLNLDVTVSAGIVSA
                     KARSININQRQSKTPIESFIQTDAAVNPGNSGGALINTNGELVGINSAIASPTGYYTG
                     YAYAIPVNIVKKVIADMVKYGSVQRAFIGISYPPEGIGEKQQEQFDKEYGTAWKTNEG
                     VFVIGVPEDGAAYAAGIKEKDIITKVNGVATPSGPQLQEQVSLYKPGDKVKITLKRNN
                     KEMVVSVTLKNKAGNTEIVKKSTILDKIGGTLETIDSKIAAANDIQGGVVVKKIGTGA
                     LSKTRVQEGFVITSVNGIEIKNLEDLKSALDKADGRIRLEGIYPGYEGSYTYPLNINN
                     ED"
     gene            complement(14163..14510)
                     /locus_tag="PLU36_01780"
     CDS             complement(14163..14510)
                     /locus_tag="PLU36_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HMZ45510.1"
                     /translation="MAIKITEECINCGACEPECPNNAIYEGGVEWAITDGTTVKGDFT
                     LLNGTTIDVEQRNEPISVDTYFIVPDKCTECQGFHEEPQCAAVCPVDCCVPDEMYRET
                     VEELLAKKDKMHI"
     gene            14601..15608
                     /locus_tag="PLU36_01785"
     CDS             14601..15608
                     /locus_tag="PLU36_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HMZ45511.1"
                     /translation="MILQERINLLKKLNNYFLENNIELQNVKQKAFQENAWFIPEFVE
                     ESIKNISNEFLEENKLNNLIQQYKIPAENIQPKRIGIVMAGNIALVGFHDFLCAFITG
                     HSVVIKPSSKDSVLIKHIVQKLAEWNSEINSIISFAEILKGCDAFIATGSNNSSRYFE
                     YYFEKYPSIIRKNRTSVAILTGKESKTELDLLAKDIQLYFGLGCRNVTKLFVPEKYDF
                     IPLLDALKKYDYYLEYHKYKHNYDYHLALLIMGNKLYMNNGSIVFTENESNYSPISQV
                     HYSYYKKNEIDYLLENIQPNQEIQCIIGYQGVPFGCSQQPCITDFADGVDTIQFLINL
                     N"
     gene            complement(15605..16645)
                     /locus_tag="PLU36_01790"
     CDS             complement(15605..16645)
                     /locus_tag="PLU36_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HMZ45512.1"
                     /translation="MRNRIHITTIIVLFMLFGKQQNVQAQDVHFSQFFEAPFLRNPSF
                     SGLFQGDIRFQGIYRSQWASVTTPFTTGSFNFEYKKPIGGADDFLTLGMQMLYDKAGS
                     ASLTTTSFLPALNYHKALSGEKNKYLSLGIMGGFVQRKFDRSKITTNSHYDGNGYNPS
                     LPDGETFLNSNFNYLDGSIGLSFNSTIGGKAEDNYFIGVAYHHLNRPKNSFYKNPDIE
                     LQAKMVVSTGIKFSLNETSYFTIHADYSKQGSFTELIGGAMYSYKIGDDIDNPKYTFH
                     VGAFMRAKDAFIPMIKLDYHPFAFSFSYDANVSQLRTASQGRGAMEFSISYTGFFDRD
                     NSSKFVPLCPKF"
     gene            complement(16724..>18313)
                     /locus_tag="PLU36_01795"
     CDS             complement(16724..>18313)
                     /locus_tag="PLU36_01795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMZ45513.1"
                     /translation="IYRPGIIMIDSAGCQVPIIGTDTVRVADVSTLIKVPQTNFCDSV
                     QLNFLDSTIVYNDTVSTYLWNFGDGVTSTIKNPTHFYNQTGSYKITLKVTTNLGCTNT
                     DTLNVPINVVQTPSIAITGDSIACINSFLQFNGTIIKSDTNAISWQWNLANGNTSVLQ
                     NPTTQQYITAGTYNIKVIAFNIAGCADTVTKNILIHPLPIINAGVDTFVCKGKTIKLN
                     ASGGVGYVWNMDSTLSCTNCASPNVFTQNSSIYKVTGTSSFGCKNEDSVAVTVIEPFT
                     FSISANDSICTGSSSQLLASGNADKFQWQPTTGLNNPNIANPIATPTTTTVYTVIATD
                     NKKCFADTGLVEIRVFPMPQFNIIESLVQSNVGNVVPLLTSNSADITKWEWIPNQWLT
                     CADCPTPNAIITDKIKYVAEASNPGGCFARDEVTIEPLCNGYNVFIPNTFSPNNDGVN
                     DVFYPRGKGLFTVRNMTIFNRWGEIVFTKRDFNANNAANGWDGTFNGKQLSADVYVYT
                     IEVICANSQTMFLKGNITLLR"
BASE COUNT         6417 a         2802 c         2755 g         6339 t
ORIGIN      
        1 tgtttcacta ccccaataaa tatcagattc tggctcccaa acatcggctc taccacctgc
       61 aaaacccaaa gttttaaaac ccattgtttc aagagctaca ttacccgtta aaatcattaa
      121 atctgcccag gaaattttgc taccgtattt ttgtttaata ggccataaca atcttctagc
      181 tttatctaaa ttgccattat ctggccatga atttaaaggc gaaaatcttt gttgccctgt
      241 acctgctcca cctcttccat ctcctattct ataggttcct gcactatgcc atgccatacg
      301 aataaagaaa ggtccgtaat ttccaaaatc tgctggccac cattcttgag agtctgtcat
      361 taatttagct aaatcggctt ttaaagcaaa ataatctaat tgattgaaag cttgtgcata
      421 gtcaaaatct ttatccatgg ggttagataa agaagagttt tggcgtaaga cttgtaagtt
      481 taattgatta ggccaccaat cttttttaat ttctttatta ctacctgtag gtaaagcatt
      541 ttttgccacc tgatggtaag ggcattgacc actatcagta tggtgtttta tttcgttttc
      601 attgctcata aaaaattatt ttgaggttga ttaattatag aatgcaacaa acttattgaa
      661 aattattgta tcaataaaat caattaactt tatataatca tagataaaac ctattaacaa
      721 atgaccctta ttcagttaga atatattata gcagtaagta atttaaaaca ctttgcaaaa
      781 gcagcaaatt tttgtaatgt tacacagcca acattaagca tgcaaataca gaaattagaa
      841 gaggaatttg aagtgaagat ttttgataga agtaagcaac cagttatacc tacaatattg
      901 ggtgaaaaaa ttattgaaca agccaaaaga attatagcag aaaaagaaaa cttaagccaa
      961 ttattaatac aaacaaaagg agaaactgct ggggagttaa aaattggaat tattcctaca
     1021 cttgcaccct acttattgcc tttgttttta aataaattta ttaaaaaata tccaaaagtt
     1081 aaattaaaaa taaccgaact taacactaat agtataataa agaaattaaa agagaataca
     1141 atagacatag gcattttagt aaccccactt aacgagaaag taatttccga aactccctta
     1201 ttttacgaag agttaatggc atatatttca aaaagaaata aggaatttaa taaaacttat
     1261 atgctaacaa aagatataga tataaaaaaa ttatggcttt tagaagaagg gcactgcttt
     1321 agaacccagg ttttaaatct ttgcgaatta agaaaaaata tgaatggcga aagccaattt
     1381 gaatatgctg ctggcagctt tgaaactttg agaaaaatgg ttgatgtaac cgatgggtta
     1441 accattttac ctgaattggc aacagaatat tttagtgcta gcgaaatgca gagaataagg
     1501 cactttaaag ctcctgcacc tgtaagagaa attagtattg ttttacatag aaattatgca
     1561 aagcaacaat tggtaacaat tttaaaagat gaaattttaa atgctattcc ggaaaaaatt
     1621 aaaaaaaata aaaaaacatt tattgttcca tttgaaagta aagaatcata aatttaattt
     1681 taaaaaatat tgcaaaatga agaagatact gatattgcta tttgttatgg ctttcaacta
     1741 caattctttt tcgcaaggaa ttgcaaccga aagtgccaaa caattttata ataaaatttt
     1801 tgagtatgac tatgtagaga aaaaaccaat atttgttgct ggcagagatt ctttaagaaa
     1861 tttttattta tcgcatttta atgcttttga ttcaatagtt accagagcca ttataaaagg
     1921 tgatacagca aagtacttaa ggatacattt tgagtatata attgatgaaa atggtgtacc
     1981 atacaattct caatttaaat atattggtag tacaagatat ggcagttcat ctggagataa
     2041 aaaattaaaa tattttaacg accttaactc gtattttaaa gaagctacaa aaacaatgat
     2101 acataaaatg cctaattggc ggccagcctt acaaaataat ataagagtaa aatgcaatgt
     2161 ggaagattat tttcaaattt ggttaggtat caatccagaa aaataacaaa accatcgaac
     2221 tgcaatttta aaacaaatga aaattttaat ccgctacatt aaaccttata aatggcttat
     2281 agcactagct tttttattag caaccattaa tcaaatcttt tcactcttag acccactcat
     2341 tgttggaaaa gtaattgatt tatttgccaa tcatccaaaa cattttgaca ccaataaaac
     2401 tttattaaga acagagaatg aatttttatt tggcagttca acttttcatg gattaatctt
     2461 ttttatttta ttgcttattg gcactgcaat ggtaagtaga gttgcaaaag cctttcagga
     2521 ttatacagtg aatgttatta ttcaaaaatt tggagcaggc atatttacag atggactaaa
     2581 acacagtatg caattgccat ttcaagaatt tgaagaccaa cgaagtggtg aaacattatc
     2641 tattttaact aaagtaagaa cagatacaga aaaatttatt tcctatttta taaatgtatt
     2701 tttaagcata attatcagca ttgtttttgt ttctatttat gcttttaaac ttcattggtc
     2761 tattgtacct gtatatatgg gaggctctat tttaattggg tttaccacta gtttattgag
     2821 caaaaaaatt aaaaatattc aaaaaaatat tgtaaaagaa actactgttt tagcaggctc
     2881 tactacagaa agtttaagaa atatagagtt agttaaaagt ctaggtttaa ccaacgaaga
     2941 agttaaaagg cttaacaata atacctataa aattttaggg cttgaactaa aaaaagtaaa
     3001 aagtttaaga actctaagtt ttattcaagg tactatgatt aattttttaa ggcaggtaat
     3061 tacatttact ttgttatggt taatttataa agatgtttta actacgggtc aatttatatc
     3121 ccttcagttt tatagttttt tcatttttgg ccctatgcaa gaaattggca atattattat
     3181 tagttacaga gaagctcagg catctttatt aaactttgat gagttgatga agaaaaagcc
     3241 agagcctaaa ccaacctcac ctttacattt aggtattatt aaagaagtta gttttgaaca
     3301 tatatctttt aaacacacct ctgcaaaata caaagcacta gatgatattt cttttaaagt
     3361 aaatgaggga gaaacagttg cttttgttgg gccaagtggt agtggtaaaa gtactttagt
     3421 taaattatta gttggtttat acaccccaca agaagggaat attaaataca ataatttata
     3481 cagcaaagaa atagattttg atgaacttag aacacagatt ggttttgtaa ctcaggatac
     3541 caatttattt gcaggaacta taaaagaaaa cctgcttttt gtaaactctt cggcaactaa
     3601 tgatgaacta aatgatgtat tggaaaaagc aagttgccaa aatttattag aacgtgcaga
     3661 taatggcatt aatactgtaa ttggagaagg aggtttaaaa ctaagtggtg gcgaaaagca
     3721 aagaatttcc attgccagag cccttttaag aaaacctcat ttattaattt ttgatgaagc
     3781 aacaagtgct ttagatagta taacagaaga ggaaattact aatactatta agcaaattag
     3841 ccttcataaa aagcaagtaa ccataatgat agcacataga ttgagtacta ttatgcatgc
     3901 agatagaata tttgttttag aaaaaggtaa aatagttgaa acaggtaatc accaagaatt
     3961 actaaatgaa aaaggtttat attttgctat gtggaggcaa caaattggag aaagaaaata
     4021 aaaaaaattc tttaaaaatg caaccaatta aactctacac cctttttgct aaaaccaata
     4081 ctaggtgctg gaaatttaaa tgaatttaaa aaaaacaaag ccacttttaa aaacttactt
     4141 tttgttttaa ttttagttaa atttatatct ggtgcaatat accattgtct atatctaggt
     4201 atatcacttc tatcaattac attattgttt tcatctttcc aaatattgtc atctccatca
     4261 aacattccat ctgcaccgta accaacagca acatttaacc aagagggtac attgctttta
     4321 ggaaaaaaag attttatatt agcacttaac caataagttt ggccattgta gtctttcaac
     4381 attctgttta aaaaactttt gccaaaaagc ttatcagctc ggttatgtaa aaacgggtct
     4441 ttataattta ctttatgaaa cgaaaatttt aaatcaactc tttgttcatc ccataataat
     4501 tcttgtgcaa taagcagtcc actaccataa caatttgcaa taaaatctcc ccagctccaa
     4561 ccccaaccaa cactataagc atctaaaaat tcaatagcag tttggtatgt tgcaccactc
     4621 aatcctccta accaaattct ttgtttacgt ggtaagccag cccaacgcca aagttccata
     4681 cttgctttac tctctacata agcactatat gcatggcctg ctttatctac ctgtaaccac
     4741 tcttcattat cattaaaaaa atgaaaatta gattgtggat aatttttata ccaagcatta
     4801 tacaatccaa tcatagcacc cccatatcct acaatattca ctgcagaaac caacctaatt
     4861 cttttataat ttcttaaatg agatggagta ttttgagaaa gcgaatcaaa aaaattttgt
     4921 tctgtaagca aattaaaacg ataaggtgcg tacattttaa ttttatctac agtagcaacc
     4981 tgtgcattgc tattttggta aatacccaac ccaaataata tgaagaaaca aaatttaaaa
     5041 taaaattttg tttccacttt tttattataa agcttaagta ctttcatcca aaaactatgt
     5101 ataaaaaagt cgttaaatat gcttaaaatt gcactcatta atgtacaggt tatcaacaaa
     5161 tgtattttat tattatttag atttttaaaa ataaaattaa ttaacaatta tggataattc
     5221 aatttcagtt aaagttaacg aatggctcgg tggaaattat gatgaaacaa ccaaacaaat
     5281 tattaaaaat ttacaagcag aaaatgaact agaacttact gagagttttt ataagaactt
     5341 agaatttggt actggtggcc taagaggcat aatgggtgtt ggcactaaca gaatgaataa
     5401 atatactgtt ggtatggcta cgcaaggctt tgcaaactat ttaaaaaaag tatatgggca
     5461 agaagaaata aaaattgtca ttgggcatga tagtagaaat aatagtagat tttttgcaga
     5521 aacaactgca aatgtttttg cagcaaatgg ttgcaaagta tttttatttg aagcattaag
     5581 accaacgcca gaattaagtt ttgccataag gcatttaggc tgtaaagcag gtgtagtatg
     5641 tactgcaagc cataacccaa aagaatataa tggttataaa gcatattgga atgatggaag
     5701 tcaattagtt ccaccacatg ataaaaatgt tattaaagag gtagaggcaa ttgcaaatgt
     5761 agatgaagtt aaatggacag gtggccaagc aaatataaca ttattgggta aggaaatgga
     5821 tgaagcatat attggtatgg ttaaagcttt aagcatttat cctgatgtta ttgctaagca
     5881 acataatttg aaaattgttt acacacccat tcacggtact ggcattacaa ttgtaccaga
     5941 aactttaaag caatttggtt ttagtaatgt aacaattgtg gaagaacaag ctacaccaga
     6001 tggcaatttt cctacagttg tttatcctaa tccagaagaa actgaagcaa tgagtattgg
     6061 attaaaaaaa gctattgaaa tagatgcaga cattttgtgt ggtacagatc ctgatagtga
     6121 tagagttgga ataggtgtaa aaaatcataa aggtgaatgg gttttaatga atggaaatca
     6181 aactttagtt ttagcttttg cttatttaat agaagctaga aaattaaaag gtttggcaaa
     6241 accaaatgat atggttattt ctactatagt ttctaccgat atggcccata atgttgcaca
     6301 gcaaaataat ataagtagct atggtgtttt aacagggttt aaatggattg ctgaattaat
     6361 tagagaaaaa gaaaaagatg aaaattatat aattggtgga gaagaaagtt ttggtttaat
     6421 gattggtaat ggaactagag ataaagatgc tgtaagtgct gttgccttat tgtgcgaaat
     6481 ggcagcttat gaaaaagaac atggaagaag cttatttgat aaacttattg aactatatat
     6541 aaaatatggt ttttattacg aatccttaat tagtattact aagaaaggta tgaacggaca
     6601 agcagaaatt gcagaaatga tggaaggata tagaaccaac ccacctaaag taattaatgg
     6661 tagcaaagta gtagaaatat tagactatga aaagcaaata ggaaaagatt ttgaaagtgg
     6721 cgatagctgg aaaatagaat tgcctaagag taatgtatta caattcatta ctgaagatgg
     6781 aagtaaaata tcggcaaggc caagtggaac agagcctaaa ataaaatttt attttagtgt
     6841 aaatactaaa ctcaactcta aaaatgagtt cgacgaaaaa tttgaattac ttcaaaataa
     6901 aattaaaggt ataattgcaa gtttgaactt gtagagtgaa tacactacac aataaaaaac
     6961 ctgaaacata atatttcagg ttttttattg ttgttcaatt ataaactaag taattattaa
     7021 agatttggct ttaatatcta ccaattctaa atcaaaaatt aattctttac ctgctaaatg
     7081 atggttagca tctaatatta cagattcctc tttaagttca ataatggtaa cattaaattg
     7141 ctgacctgca ccattatgca ttgttaattg catgccaact tctggagtta aatctggagg
     7201 gaattgagct ttaggaaaat ccataatcat atctgggtta ctttcaccat aggcttctgc
     7261 tgcaggtatg ttaatagttt tcttttctcc aacactcatg ccagttacac cattatcaaa
     7321 accaggaatt accattccgc tacctaattc aaattgtaat ggttctcttc cttcactact
     7381 gtcaaaagtt gttccatctg ttaatctacc atgataatga attttaacag tatcgccttt
     7441 ttttgcttgt tgcattattt tatttttaaa atgaataata taccgttttc tttcaaataa
     7501 atttatttgc acctattcgg gcagccaaag tacaccctaa tttattaacc tacaagaaat
     7561 tttaatttat gtgcataaaa cagcttatag caacataaca aacagaattt ctttaccagc
     7621 tattggtatg taaattgtat tcccataaca cttttataaa ttattttttc ctaaaataaa
     7681 tatttcacaa aagcacttaa aactcttatt tggttatttc atttatacgg ttacatttgc
     7741 aaaaaattaa ggactatgtt ttggacatta gagttggcaa gttatcttga agaagcccct
     7801 tggcctgcta caaaagatga actaatagat tatagcatac gtagtggtgc ccctattgaa
     7861 gtagttgaaa atttgcagga attagatgac gaaggaacag aagtttatga gagtattgaa
     7921 gacatttggc cagattatcc aagccaagat gactttatgt ttaacgaaga tgaatattaa
     7981 ataattaacc accttttggg tggtttttta ttgccatact tctaatataa ttttcttatc
     8041 tggtttacct tcattagaaa ttacaatatc atacctcttg ggtattattt tttctaactc
     8101 tttttccgtt aatccttttt ttctaataat tagtttagat atattattct cttgttcaat
     8161 atctgttgct gttacagtat aagagctatc atttaatgta ttaaatgcat aactaaatgg
     8221 cattgaactt tcaggaatct caaaatcttc ctcttctaat tctgtatcat taatttttgg
     8281 cttactgttt ttaggagaat tttttttgct attaaatggc ttacctactt cagaatttgt
     8341 agaaagtttt ctcttattat catttgtaga aatgctttct atatctgctg gctctttaac
     8401 caatttttca aataatatat attgttcttg ttgattattg ctacttttat tttgtgctaa
     8461 accagtatgt gataaggttt taacttcatt tttaattgtg ttacttaata atctatttgt
     8521 tgctttatta attgattcgt tttgaatatt accagaaaag aaaaatgtaa aagatagcag
     8581 tgcaaataac ataaaaacaa agccaaatcg taatgcagaa taattatttt tagcactttt
     8641 tgaatttgta attctattga ctctttttaa taactcattt ttatttgata tagcagccat
     8701 accaaattga tagggcctaa ggttactcat tgcaatttgt agcaaagcat ttgcatataa
     8761 attggggcaa tatttaaatt gcaaaaccca gtcatcacaa ctattttctc tctcttgctt
     8821 tatatgctca cttaacaatc ttgaaaaagg attaaagaat aaaactattt caactattag
     8881 aagtaaaata ttaactaaaa aatcaaacct tttaatatgt gctaattcgt gtaatattac
     8941 tgcctctatc tgataggtag ataaattatt aatacttgct aaaggcaata atataatagg
     9001 cttaaaatac ccaattgtta gtggtgtgtt taccaattcg cttaagtata ttttaacttc
     9061 tttatttaat cctaatgcaa ttgccatttc attaacaaaa actctccatt ctgcatttac
     9121 tttatgtagt ttattaattc ttaaattgtt tgtatgatta aaccctataa tgacttttac
     9181 aagtagaatt attgttacta ataaatatac aacagataag aaaggcaatg ctttttctac
     9241 ttttagaatt aggggtataa aaaatgaatt aaaattattt gattgaagga ataggccatt
     9301 ctttaagtta gatgaagtag aaaaaaagtt atagcagaaa gtatatataa aactacagaa
     9361 accaattatt tgggctacaa cagcaattag atatttatgt ttggaaggta acttcaagaa
     9421 atttgtgaaa agaatataaa gcaaccagat tattgccact tgccataaac tatttattag
     9481 tgtatagcct aaagattgta ataaaaagga tgtagcaatt ggttgcataa ataaagctat
     9541 ttatttttta gcctattcaa tattttttct atttcttcca attcatcttt acttggtttg
     9601 ctgttaccta aagcttgcat tgctaattgt acagatgagc ctccaaaaag cgtttcaacc
     9661 attttatgca taaattgttt ctgtgtagat tcttttgaaa cgttgggtgt ataaatatgt
     9721 actttgcttg tatcatctct aattaccaaa ctcttttcaa acattatttg taataatttt
     9781 agagttgttg tataaccact ttctttgtgt aaactcaaaa tttcatgcac ctctctaaca
     9841 ctagcagatt tcttatccca taatattctt aaaatttcca attcactttc ggtaggttta
     9901 attttttgct tattaaacat aattacgatt ttattcgtaa gcaatatact ttataaattt
     9961 taattagaat tataaaatta tataaatggt agtaaaaaca aactgcccca atatttgggg
    10021 cagtttgaat ataaattaaa gcgaatatta ttgtttttca actaacttaa gttccactct
    10081 tctatttagt gctctgcctt gtgctgtttt attatcagct acagggcttt cttgaccaaa
    10141 tccttttgca gataaacgat tttctgcaat accttttttc ttaaagtagc ccattacagc
    10201 atttgcacgt gattgagata attttaaatt acttgcaggt ttacctacat tatcagtgta
    10261 gccttcaata tttaaaccta atgtttcatt gcctttttct tttaataaag ctacaatagt
    10321 atctaatgat ttatagcttt ttgttaataa ggcagattta ccagtagcaa aataaatatt
    10381 tttagcgtat aactgaacct tttgtttttt ctcttctgtt aaaactgggc agccattatt
    10441 gtgtgcaggg ccagcttctg tagggcattt gtcttcttca tcattaatgc catctttatc
    10501 cgtatctggt ataggacaac cttgataacg agctaaacca gcaactgtag ggcatttgtc
    10561 ttcttcatcg ttaataccat ctttatccgt atctggtatt gggcaacctt gatattttgc
    10621 aacaccatat actgtagggc atttatcttc ttcatcatta atgccatctt tatctgtatc
    10681 tggaattggg caaccttgat attttgcaat accatacact gtagggcatt tatctaaact
    10741 atcaacaatg ccatctttgt cggtatcttt tggttcaact tttttaggag gcattggagg
    10801 ttctactggt tttgcagcag gtttatcgca taaaggagca cctaagccaa ttgaatagtt
    10861 taagtgatag ttaccagttt tttgagttac agggtaacga taagaaacat ctgtaaataa
    10921 aaatgcttcg ctttggccta aattaaattg taagcctgta ctaactggaa tgtaagcacc
    10981 aaaatgtgag ccacctaatg ctgttgcacc aacaccacca gaaacataag gaacaactat
    11041 atacttatca gttaataatt ttagtcttaa agtagcatct aactctaata aaaacttttc
    11101 tttacccaat tttggagcat ctaaaaaagg ataatcaata aaactaccgc ctaaattgga
    11161 tgcaaaatcc atgtgattag aaatgccttt aatatacgaa actcccaatc ctggtaccat
    11221 atcagcaaat tgagcccatt gcttgttacc aattacagaa gataaagatg aagttccaat
    11281 acgactaggt gttaagaaat ctttcataaa aaagttaaca cttagtgttg gccttttttt
    11341 ataatcagct ttttgatttt gagcaaaaga agttgttgtt attaatccaa caacaaaaag
    11401 taaaataatt ttcttcataa ttacatttta attaattgtt gcaaaattat gggtatatga
    11461 taaaataaac gaattttaac tttgaatatc ttaataagtt tatacatgaa caaaaaatat
    11521 tatatatttt tcctactttg ctttatcatt tcttgcaaat ctaataagca aatattttct
    11581 aaaaatgagt atggtttaat agttctcacc actccaaaac aatatttttc taccgtatca
    11641 ttagatagca gtaaactatt aaaaaggctt aatggtttta taccaaatat tgtttttgat
    11701 tggagatatg caaccacaaa taattttaca aaacaaatat tatacacaaa cccagatgct
    11761 tatttaagaa ttgaagcagc caataattta aaaaatgttg ctatagagtt agaaaaaata
    11821 ggattaggaa taaaagtttt tgatgcttat cgtccttatt ctgttacaaa aaaaatgtgg
    11881 gaggttgtgc cagacgatag atatgcatct gaccctgcaa aaggcagtgg gcataatcgg
    11941 ggtattgcta tagacttaac attgtataat ttagaaacag gaactgaact tgaaatgcca
    12001 acctcttacg ataattttac tgaaaatgct catgctaatt atcagcaact aagccaaata
    12061 gttttaaaaa atagaacgat tttaataaat actatgcaaa tgtttggttt taaagtatta
    12121 gatacggaat ggtggcattt ttatttacca aactcttcta aatacgaatt aatggattat
    12181 gattttaaaa ctatgagcaa aatgtgtaat gtaaatactc ataaataaaa atgctaccta
    12241 aaatttaggt agcattttat gtttaaaatg gtgagttaaa tttattagtc ttcgttgtta
    12301 atgtttaaag gatatgtgta agaaccttcg tagcctggat agataccttc taatctaatt
    12361 cttccatcag ctttatccaa tgcagatttt agatcctcta aattttttat ttcaatacca
    12421 tttacagaag taattacaaa gccctcttga acacgtgttt tggataatgc accagtacca
    12481 atttttttaa ccactacacc accttgtata tcatttgcag cagcaatttt tgaatcaatt
    12541 gtttctaatg ttcctccaat tttatctagt attgttgatt tttttactat ctcagtattt
    12601 cctgctttat ttttaagtgt tactgataca accatctctt tgttgtttct tttcaaagta
    12661 atttttactt tatctcctgg tttatataaa gatacttgct cttgtaattg tgggccacta
    12721 ggagttgcta caccatttac ttttgtaata atatcttttt cttttatacc agcagcataa
    12781 gctgctccat cttctggtac accaattaca aaaacacctt cattggtttt ccaggctgtg
    12841 ccatattctt tgtcaaactg ttcttgttgt ttttcaccaa taccttctgg tgggtaagaa
    12901 atacctataa aagctctttg aacagagcca tattttacca tgtcggcaat tacctttttt
    12961 acaatattta ctggtattgc ataagcatag cctgtataat agccagttgg agatgcaata
    13021 gcactattga tacctactaa ctctccatta gtatttatta aagctccacc actgttacct
    13081 gggtttacag cagcatctgt ttgtataaaa ctttcaatag gtgttttact ttggcgttga
    13141 ttaatattaa tgcttctagc ttttgcacta acaatgcctg cacttactgt tacatctaag
    13201 tttaatggat atccaatagc taaaacccat tgtccaagtt ttacttcatc actatttcca
    13261 taaattaaat aaggaaaatt attgccttca attttaatta ctgcaaggtc ggtacttggg
    13321 tctgttccaa taatactggc agtataagtt tttttatcag ttaaggtaac ttttatttca
    13381 tcggcaccat ctacaacatg gttattagta acaatatatc catctttact tacaattaca
    13441 ccgctaccac ttgcttgctg ttctggtatt acttgcatac gtggaccatt aaagaactca
    13501 tcaaacatat cgccaccaaa catatcacta aatgggtttc tgctacgagg ggcattattg
    13561 cttatttgct ttgattttgt tttggttttt atatgtacca cagcaggtgt agcacttgtt
    13621 gctgctaaag aaaaatctgc tccaattggg gcatttgtat tatcaaaaaa tcttgcatag
    13681 tttacaggta gtttaccttc ctcttgagtg cctgcatatt tgttttgcat atacttatca
    13741 tatcctacca cactcaaaac agcagtagca gcacttacta ttactactgt aaataaattt
    13801 ttgagattgc tattcataat tgttctttta aaatgattat tctgatttaa aattaatgat
    13861 taaaatattt caaataccat gcctttcgta tgcaagtaaa taaaccagta gaaatgtcag
    13921 tacatttaat acatcattta acattctttt acatagcatt taacaagttg atgtattttg
    13981 agataagaat ataaaattaa aaaagcaaaa gatgctttta gttcttttgc ttttaaaagc
    14041 aggaaatatc acccgccgtt gctaaaagta gtattgctac tttttattgt aatactaagg
    14101 taataatgtt ttgatattaa ttgctttttt tagttataat attatgcaca ttgcattgtg
    14161 ctttaaatgt gcattttatc tttcttggct aaaagttctt caactgtttc tctatacatt
    14221 tcatctggca cacagcaatc tacagggcaa actgctgcac attgtggttc ttcatgaaac
    14281 ccttgacact ctgtacattt atctggtaca ataaaatagg tgtccacact aattggttca
    14341 ttacgttgtt ctacatcaat ggtggtgcca tttaataatg taaaatctcc tttcactgta
    14401 gtaccatctg ttattgccca ctctactcct ccttcgtaaa ttgcattgtt aggacattca
    14461 ggttcgcaag ccccacagtt tatacattct tctgtaattt ttattgccat aataataaat
    14521 tttatcttgg ttatgaatag atagcaatag aaaagtattt tacaacctta cctttgccaa
    14581 ctcaaaaata aagttaagaa atgattttac aagaaagaat aaatttatta aaaaaattaa
    14641 acaattattt tcttgaaaat aatatagaac ttcaaaacgt aaaacaaaag gcatttcaag
    14701 aaaatgcttg gtttatacca gagtttgtgg aagaaagtat aaaaaatatt tcgaatgaat
    14761 ttttagagga gaacaaatta aataacttaa ttcaacaata taaaattcct gcagaaaata
    14821 tacaacctaa aagaattggt attgtaatgg ctggtaacat tgcattagtt ggttttcatg
    14881 attttttatg tgcatttata acaggacatt ctgttgttat taagccttct tctaaagatt
    14941 ctgttttaat aaaacatatt gttcaaaaat tagctgaatg gaatagcgaa attaatagca
    15001 taataagctt tgctgaaatt ttgaaaggtt gtgatgcgtt tatagcaaca ggcagcaaca
    15061 acagcagtag atactttgaa tattactttg aaaaataccc aagcattatt agaaaaaata
    15121 gaactagtgt tgccatactt actggcaaag aaagtaaaac cgaattagat ttactagcta
    15181 aagacattca actatatttt ggattaggtt gcagaaatgt tactaaactt tttgtacctg
    15241 agaagtatga ttttatacca ctattagatg cattaaaaaa atacgattat tatttagagt
    15301 accacaaata caaacataat tacgattacc atttggcatt attaataatg ggtaataaat
    15361 tgtatatgaa taatggctct attgttttta ctgaaaatga aagtaattac tctcctatta
    15421 gtcaagttca ttatagttac tataaaaaaa atgaaattga ttatttacta gaaaacattc
    15481 aaccgaatca agaaattcaa tgtataatag gctatcaagg tgtgccattt ggttgttctc
    15541 aacaaccttg tataaccgat tttgcagatg gagtggatac catacagttc ttaataaatt
    15601 tgaactagaa tttagggcaa agaggaacaa attttgagct attatctcta tcaaaaaaac
    15661 ctgtatagga aattgaaaac tccatagcac ctctaccctg gcttgcagtt cttaattgag
    15721 aaacattagc atcgtaacta aatgaaaatg caaaagggtg ataatctaat tttatcattg
    15781 gtataaaagc atctttagcc ctcataaatg cacctacatg aaaagtgtat ttaggattgt
    15841 ctatatcatc gccaatttta taagaataca ttgctccacc aattagctct gtaaagctac
    15901 cttgtttaga ataatcggca tgaattgtaa aataagaagt ttcatttagt gaaaatttga
    15961 tacctgtaga gacaaccatt ttggcttgta attctatatc ggggttttta tagaaagaat
    16021 ttttaggacg atttaaatgg tgatatgcaa cacctataaa ataattatcc tctgcttttc
    16081 cgccaattgt tgaattaaaa ctaagcccta tacttccatc taaataatta aaattagaat
    16141 ttaaaaatgt ttctccatct ggtaaagaag ggttataacc attaccatca taatggctat
    16201 ttgtagtaat cttacttcta tcgaattttc tttgtacaaa gcctcccatt atgccaagag
    16261 aaaggtattt atttttttct ccacttaatg ctttatggta atttaaagct ggtaaaaaac
    16321 ttgtagttgt taaagaggca gagcctgctt tatcatacaa catttgcata cctaaagtta
    16381 aaaaatcatc tgctccacca attggttttt tatattcaaa attaaaagag ccagttgtaa
    16441 aaggagtagt taccgatgcc cattgacttc tatatatacc ttgaaaacga atgtctcctt
    16501 gaaataatcc tgaaaaagaa ggatttctta aaaagggagc ttcaaaaaat tgtgaaaaat
    16561 gaacatcttg tgcttgtaca ttttgttgct tgccaaaaag cataaaaagc acaataattg
    16621 tggtaatatg gatacggttt ctcattttta gagatattgg gtacaataca aacgtacttt
    16681 ttattttatt tattgtataa acccttttca taatagtgct tttttagcgt aaaagcgtaa
    16741 tatttccttt taaaaacatt gtttggctat ttgcacaaat aacttctata gtataaacgt
    16801 aaacatctgc agaaagttgt ttaccattaa atgttccatc ccagccattg gcagcattat
    16861 tggcattaaa atctctttta gtaaatacaa tttcgcccca acgattaaaa atggtcatat
    16921 ttcttactgt aaataaacct tttcctcttg gataaaaaac atcatttacg ccatcgttgt
    16981 ttggtgaaaa tgtatttgga ataaaaacat tgtatccatt acaaagtggt tcaatagtta
    17041 cttcatctct ggcaaagcaa ccaccaggat tagatgcttc ggctacatat ttaattttat
    17101 ctgttataat tgcattaggc gttgggcaat cggcacatgt aagccattga tttggtatcc
    17161 attcccattt agttatatct gcagagttgg aagttagtaa aggaaccaca ttgccaacat
    17221 tagattgaac taatgattca atgatattga attgaggcat ggggaaaacc ctaatctcaa
    17281 ccaatcctgt atcagcaaaa cactttttat tatcggttgc tataacagta taaacagtag
    17341 ttgtagttgg cgtagcaata gggttagcaa tatttggatt gtttaatcct gttgttggtt
    17401 gccattgaaa tttatctgca ttaccagatg ctaaaagttg agaactgcta ccagtacaaa
    17461 tactatcatt tgctgaaatt gaaaaggtaa atggttcaat tactgttact gccacagaat
    17521 cttcattttt acagccaaaa ctgcttgtac ctgttacttt ataaatagaa gagttttggg
    17581 taaatacatt tgggcttgca cagttagtac aacttaaagt agaatccatg ttccaaacat
    17641 accctacacc accacttgca tttagtttaa tagtttttcc cttacaaaca aaagtatcca
    17701 caccagcatt tattattggc aaagggtgta ttaaaatatt ttttgtaaca gtatcggcac
    17761 agcctgctat attaaatgca ataactttaa tattataagt gccagccgta atgtattgtt
    17821 gtgtagtagg gttctgaagt acagaggtat ttccatttgc caaattccat tgccaactaa
    17881 ttgcatttgt gtcacttttt attattgttc cattaaattg tagaaaacta ttaatgcaag
    17941 caattgaatc tcctgtaata gctattgaag gtgtttgcac aacgttaata ggaacattca
    18001 gtgtatcggt attagtgcat cctaagttgg ttgttacttt tagagtaatt ttatagctac
    18061 ctgtttgatt gtaaaaatga gtagggtttt ttattgttga ggtaactcca tctccaaagt
    18121 tccataaata ggttgaaaca gtatcgttat aaacaatagt tgagtctaaa aaattaagtt
    18181 gaacagaatc gcagaaattt gtttgtggta cttttataag agtgcttaca tctgctactc
    18241 ttactgtatc tgttccaata attggcactt gacaccctgc tgaatctatc ataataattc
    18301 caggcctgta aat
//
LOCUS       DAOQPK010000019        18099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_1553_length_18099_cov_4.804256, whole genome shotgun sequence.
ACCESSION   DAOQPK010000019 DAOQPK010000000
VERSION     DAOQPK010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 18099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18099
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1553_length_18099_cov_4.804256"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /gene="atpA"
                     /locus_tag="PLU36_01800"
     CDS             <1..1201
                     /gene="atpA"
                     /locus_tag="PLU36_01800"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HMZ45514.1"
                     /translation="YEMPLERKAPGVIYREPVTEPLQTGIKSIDAMIPIGRGQRELII
                     GDRQTGKTAIAIDTIINQKEFFDAGKPVYCIYVAIGQKASTVAGVMKTLADNGAMAYT
                     TIVAASASDPAPQQFYAPFAGAAIGEFFRDTGRPALIIYDDLSKQAVAYREVSLLLKR
                     PPGREAYPGDVFYLHSRLLERAAKVINNDSVAKNMNDLPESIKHLVKGGGSLTALPII
                     ETQAGDVSAYIPTNVISITDGQIFLEGNLFNAGIRPAINVGISVSRVGGNAQIKSMKK
                     VAGTLKLDQALYRELEAFSKFGGDLDAATKNVIDKGARNVEILKQNQYTPFSVEKQIA
                     IIYLGTQGLLKDVAVKNVKAFEEMFLTEMQNKLPEVLAEFKKGQLNNDSIDKMVNLAK
                     GLIPQYK"
     gene            1332..3251
                     /locus_tag="PLU36_01805"
     CDS             1332..3251
                     /locus_tag="PLU36_01805"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HMZ45515.1"
                     /translation="MKPYWNIKITVRVYILVLTIFTFFRIILFINEYGRLDETATLTN
                     RLLAFFMGVRFDIVISGYILFFPFVALSILNITKDSLLVKRIIFYFIFILFTLAFLVC
                     AADIPYFNQFFSRFSVTAFEWLNSPMFVLKMIIQEPRYWLFFILFVVVVIIFFKRLQH
                     IFKSNELTIIKNKKWKFGLISLLAAGFIFLGVRGRIDEKSPIRVGTAYFCNNAFLNQL
                     GLNPNFTLMRSWIDAMEEENKLVNLMDEKIAIQNTQKYLQITNPSQTLPISRIENDTV
                     TNKHNYNVVFIIMESMSAAKMKRHGNAKNLTPFLDSISTKGLYFENNYSAGIHTFNGI
                     FSTLFSYPALFRQHPMKESGMFKYNGIASTLKSNNYSTIYFTTHDGQFDNVEGFLNGN
                     DYEQVISKEDYPFGKAPTTLGVPDDYMFEFSIPLLNNLHQKNKPFFAAFMTASNHRPF
                     YIPDYFSSKFTDEKDKIVEYADFALSKFISMASHQEWFKNTIFVFVADHGSPISSKYD
                     LSLDYNHVPLLFYAPEIITKHKTYSTMSGQIDIYPTTMGLLGLTFTNNTLGVNLFKQQ
                     RPYIYFNADDKYGVIDSNWLLIVRNDKFKGLYKYRNNNVTNYANEYKDIVNRMQTYAE
                     SNLQTFQYLLKNKKL"
     gene            3327..4769
                     /locus_tag="PLU36_01810"
     CDS             3327..4769
                     /locus_tag="PLU36_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HMZ45516.1"
                     /translation="MNIIVNNIESLFYNWQKSAPLKIEKLQQSGSDRIYFRVFGSHQN
                     YIATYNLNIQENKSFIAFARHFKNKGLNVPEIYCHNEDYTIYIQQDVGEKSLLNLLEE
                     YGQNDFVFNFFKESLKQLAAFQIIGNEGMDYSLCLTAQEFGKEAILSDLLYFKYYFLD
                     TLKLPYDKQAMLKDFDALSTYLSYSEHKYFMFRDFQSRNIMLENNKISFIDFQGGMKG
                     ALQYDVASLLWQAKAALSNEWKDALLEYYMDEAEKLLHQKIERNSFISQYHGYVLIRL
                     LQVLGAYGFRGLFERKAHFLTSIPLGLLNLKYFIENKKVGITTPEFDRVLKIIISNNI
                     FEKFTPQQANDETRLLVTINSFSYKNGIPADTSENGGGFVFDMRGILNPGRQEVYKYL
                     SGKDKPVQDFLDQQTKMPNFLNSVWDLIDINVENYLTRDFENLMINFGCTGGQHRSVY
                     AAEQTARHLRNKYKLKVILNHTNQANWLRK"
     gene            4779..5510
                     /locus_tag="PLU36_01815"
     CDS             4779..5510
                     /locus_tag="PLU36_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HMZ45517.1"
                     /translation="MSSTLNKAMLLAAGLGTRLQPFTNHHPKALAIVNNKTLLQRNIE
                     YLQQFGISNVIINVHHFASQITDALQQNNGWGSNITISNESNEVLETGGGLQKAAWYF
                     DNTEHFLLMNVDILTNLNLYELLNAHIQNKALATLAVTDRESSRYLLFNENNKLCGWR
                     NVKTGEEKIPVPTNPLFQKAFSGIQIIQTKLLSLIQQKGKFSMIDVYLSLAKENNIQC
                     FNHSHTKLIDVGKPENLERAASMFE"
     gene            complement(5522..6091)
                     /locus_tag="PLU36_01820"
     CDS             complement(5522..6091)
                     /locus_tag="PLU36_01820"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HMZ45518.1"
                     /translation="MFIVFSSAIAQTNYKQYIKQYQQEYVAKHEVVVGDDKQFFSFFK
                     ADENFKTNSTVTLLSDTIGFIMKTSGTKDKKFYRYAIINFKLYKKPFRLTLYSNKIIK
                     DSSQTNAYMFLPFTDNTCGNESYSGGRYLDFELADIKQNNLSLDFNKAYNPYCVYALG
                     YNCPIPLRENHLQINIRAGEKMFKGKKKK"
     gene            6266..7258
                     /locus_tag="PLU36_01825"
     CDS             6266..7258
                     /locus_tag="PLU36_01825"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HMZ45519.1"
                     /translation="MKVAVVGATGLVGTKMLQVLAERNFPVTEIIPVASKKSIGKEIE
                     FKGKKYKVVSMEDAIKAKPALALFSAGGSTSLEWANKFAEANITVIDNSSAWRMDESK
                     KLVVPEVNATVLTVNDRIIANPNCSTIQMVVALQPLHKKYKIKRVVVSTYQSVTGTGM
                     KAVDQLFNERNNKEGAMAYKYKIDLNAIPQIDVFLDNGYTKEEMKMVKETCKIMQDET
                     IKVTATTVRIPVIGGHSESVNVEFDNEFELEEVKEILTNSPGIIVQQNDAEQFYPMPI
                     WAHEKDEVFVGRIRRDESQPKTLNLWIVSDNLRKGAATNAIQIAEYLYKNGFLN"
     gene            7264..7872
                     /locus_tag="PLU36_01830"
     CDS             7264..7872
                     /locus_tag="PLU36_01830"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HMZ45520.1"
                     /translation="MLLFIHPDNPNPRNIKTVVECLKDGGTIIYPTDTIYGLGCSIYE
                     HKAIEKVAKIKNVSPEKAHLSFICYDLSDLSKYTKSISTPLYRMLKNYLPGAYTFILP
                     ASKEVPKILHSKKKTIGLRVPDNNIARTIIKELGSPILSASLPGEMVEEYTDPELMYE
                     NFKHLVDIVIDGGAGGMIPSTVVDCSQEPYQLIRKGLAAWND"
     gene            complement(7930..9321)
                     /locus_tag="PLU36_01835"
     CDS             complement(7930..9321)
                     /locus_tag="PLU36_01835"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HMZ45521.1"
                     /translation="MIKRLLLASALFCFITSNAQAPVGYYDSVKNKNCKELKSTLKSI
                     ITNGHTAQGYGDLWDQYKKTDTIKNPSGNNYIIWDMYSFKANGTADYYYTPGTDQCGS
                     YNSEADCYNREHSFPASWFNDEAPAYTDYNHLFPTDGWVNNKRSNYRFGEVASPSYTS
                     SNGSKLGSSATAGINGTVFEPIDMYKGDFARAYLYMVTRYQNRITTWYGYNTDGALTL
                     SNDTFPSVNINYLKLMLKWHNQDPVSEKERYRNNGTYNFQGNRNPYIDSPQYVTRVWN
                     STCSGLAALPINIVLFSGKLKNGNVFLTWQTENAINFNSFNVERSFNGKDYTAIGTVP
                     ANNKNDYSFTDNEENIRGRRVYYRLKQIDNDGSFQFSEVFSIHIPLNSKYTIYPNPAM
                     NFIIIEKTGINNNLSHQIQLTDLAGKILISKYYANANSQIILPIQHLPNGTYILRFTA
                     GNESFIQKIVKRN"
     gene            complement(9332..10888)
                     /locus_tag="PLU36_01840"
     CDS             complement(9332..10888)
                     /locus_tag="PLU36_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HMZ45522.1"
                     /translation="MPLAKIMWVDDEMESLQSQKIFLENKGYEVITFSNGFDAIEYVK
                     ENITDVVLLDESMPGITGLETLSKIKEINQQLPVVMITKNETETLMDEAIGSQIADYL
                     LKPVNPNQVLLSLKKIIDNKRLVAEKTTTNYQQQFRELFMALNNNPNYNEWMELYKKL
                     VYWELEMEKSDSPEMQEVLATQKQEANTEFFKFISKNYSKWMHPKTTDAPIMSHNLFQ
                     FKVLPHVEKGTATFFILIDNLRYDQWKAIQPIFTENFRILEEETFYSILPTATQYSRN
                     AIFSGLLPIDIEKKFPLEWKNDDEEGGKNLFEEKFFRQQLKKLNKDDLKVSYTKVVHH
                     NDGQQLVNNIHNLLNNDLNIIVYNFVDMLSHARTEMEVLKELAGDEKSYRSVTKSWFE
                     HSALNQALKKISGKKINLIVATDHGTVRVKTPYKVIGDKQTTANLRYKHGRNLHYEAK
                     EVLAFKDPREAGLPVPNVNSSFIFAKEDGYLCYPNNYNHFVNYYKNTFQHGGISLEEM
                     IVPIIKMVSK"
     gene            complement(11047..12414)
                     /gene="tig"
                     /locus_tag="PLU36_01845"
     CDS             complement(11047..12414)
                     /gene="tig"
                     /locus_tag="PLU36_01845"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HMZ45523.1"
                     /translation="MATVTRENIGNLTDKLTVNLSKEDYLKNFETSLKKYAKTANIPG
                     FRKGMVPAGLVKKMYGQSVFTDEVLRTVETQLNEYLTKEKLEIFAQPLPLDSDARALD
                     MNNPMDYAFAFEIGLKPSYTINTNFNVTRYKINVTNEMLNDEIDRLQRRFGKMTEPAT
                     VTDDETMLNVTFTEVDKEGNMVENGINKPNSVLVKYFAPAFRANLLGKKLEDVIDVHL
                     ATAFEDKELDVILSDLGLDKNIAGVADKNFKLTITKIGLVEKAAINNELFIAAYPNKE
                     IKTEEEFNNAVKEDIEAYFTQQSNNQIHDQIYHQLLDNTSIDFPEAFLKRWIQTNSET
                     PKTIEEAEQEFPSFKNSLKWTLITSQLSEENKIEVLPEDLRNFAKQQLFQYMGNQLGI
                     LDEQQQWIEDYADKMMKDKKFVEDSYHRISTEKLFNILAQKVNATEESISAEDFASKL
                     HHHHH"
     gene            complement(12511..12592)
                     /locus_tag="PLU36_01850"
     tRNA            complement(12511..12592)
                     /locus_tag="PLU36_01850"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12556..12558),aa:Leu,seq:tag)
     gene            12807..13013
                     /locus_tag="PLU36_01855"
     CDS             12807..13013
                     /locus_tag="PLU36_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45524.1"
                     /translation="MDKSKMIKAYCLKTKEKNVPMHDAVISKTSRGGYMASGNDGKGN
                     KMSAMLGEAKALQAIADGVAKKGF"
     gene            complement(13072..13686)
                     /locus_tag="PLU36_01860"
     CDS             complement(13072..13686)
                     /locus_tag="PLU36_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HMZ45525.1"
                     /translation="MGNIKNIIFDLGSVILNIDYLLTEKAFIQLGITNFSAFYNQHKS
                     SLLFEQLETGNISPEEFYNTFRKITKNSLSNLQIQTAWNAMLLNLPKERINWLLEIKT
                     KYNIFLYSNTNQIHYNCFIDLANNATPSKNFDELFIKAYYSHICGFRKPYKESYLKIV
                     EEQNLQPKETLFIDDTLVNIEGAKLAGLQTLHLLYPKTIIDLSL"
     gene            complement(13745..15265)
                     /locus_tag="PLU36_01865"
     CDS             complement(13745..15265)
                     /locus_tag="PLU36_01865"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HMZ45526.1"
                     /translation="MKIVLTTLCYFIVAATFAQTNYNVEKFANTITQKDLKQHLTYIA
                     GNETEGRETATVGQRKAALYIEEQFKNLGLKPGNANSFYQYFKVYQDVLHSSSLKIYD
                     AHFSLNNHYTIQPNSTSNGSISINQIVFASYGLTDKTMNNYENIDIKGKWVMIIESNP
                     EDIDKEKSGADSRKSFTKIKTALDKGAIGVLLVSKNFDNPKYSTLPITGSMYVKKQSN
                     NLSTPIISISYQAAASILTKTIVNFSDLKNIETGTYTTQIVLNNNKETKTLESSNVVG
                     FLEGTDKKEEFVVLTAHYDHLGKKGDVIYYGADDDGSGTVTIIEMAEAFIKAKKKGYA
                     PRRSIVFMTVSGEEKGLWGSDYYTQHPIYPLAKTTVNLNIDMIGRIDPTYKGDSLNYV
                     YIIGDDKLSSDLTPISNNVNKLYKLELDRKYNDPKDPNRFYYRSDHYNFAKNGVPVIF
                     YFNGVHKDYHRPSDTVDKINFDVMTKRARYIFATAWEIANRNDMLKRDIPLNMPAR"
     gene            complement(15384..15785)
                     /locus_tag="PLU36_01870"
     CDS             complement(15384..15785)
                     /locus_tag="PLU36_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ45527.1"
                     /translation="MTNELLIGKNILIVEDNAINQMLVKYAFAKSGANTIVAENGSQA
                     LELMNQNKFDAILMDIYMPELDGYQTTDIIRNQLHSSIPIIAMTALAMKGEEEKCLAL
                     GMNGYISKPFSSETLFQEIKRVLEFTEAIAS"
     gene            15970..16710
                     /locus_tag="PLU36_01875"
     CDS             15970..16710
                     /locus_tag="PLU36_01875"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMZ45528.1"
                     /translation="MKKIFLLFFITIGFTSYVCSQQVEKASKKKYDLTNRTSDHFMLQ
                     YGMDRWSGAPDSLNVKGSSRHFNFYFMLDKPFKNSPKFSVAYGVGIGSSNIFLDKRYA
                     DIKSNTTVLPFKVSYPGTDSSYFNKFKMTTIFLEAPVELRFYSDPENTNKSFKAALGV
                     KVGTLLKAYSKGKDLKNKSGQSIFGNRFIEKQYDKHFFNGTRLAFTARIGYGIFGLHF
                     SYQITQAIKEGAGAEVKPYSIGLTIGGL"
     gene            complement(16793..17833)
                     /gene="dusB"
                     /locus_tag="PLU36_01880"
     CDS             complement(16793..17833)
                     /gene="dusB"
                     /locus_tag="PLU36_01880"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HMZ45529.1"
                     /translation="MVKIGDNINLNDFPLLLAPMEDVSDPPFRAICKDNGADLMYTEF
                     ISSEGLIRHAIKSKRKLDIFDYERPIGIQIFGGDEEALALSAKIVDTVNPDLVDINFG
                     CPVKKVVTKGAGAGVLKDVDLMVKLTQAVVKSTKLPVTVKTRLGWNDDTKNIEEVAEK
                     LQDVGIKALAIHGRTRCQMYKGEADWTLIGKVKNNPRINIPIFGNGDIDSPQKAKQYK
                     ERYGVDGIMIGRAAIGYPWIFREIKYFLQTGQELSPPTLLERINVCKKHLLKSVEWKG
                     NKVGIFEMRRHYTNYLKGLPNIKEFRGKLVTMIEVEEIIQTLEEIYNYYNGFEIEKTP
                     IELINYHEKCSL"
BASE COUNT         6020 a         2641 c         2783 g         6655 t
ORIGIN      
        1 atatgaaatg ccattagagc gtaaagcacc tggcgtaatt tatcgtgagc cagtaacaga
       61 accattacaa actggtatca aatctataga tgcaatgatt cctattggtc gtggccaacg
      121 tgagttaatt attggtgata gacaaacagg taaaactgca attgctattg atacaatcat
      181 caatcaaaaa gaattttttg atgcaggcaa accagtttat tgcatttatg ttgcaatagg
      241 acaaaaagca agtactgttg ctggtgttat gaaaacatta gcagataatg gtgcaatggc
      301 ttatacaaca atagttgcag cttctgcatc tgaccctgct cctcaacaat tctatgctcc
      361 atttgcaggt gctgctattg gagaattttt tagagatact ggtcgtccgg cattaattat
      421 ttatgacgat ttatctaaac aagctgtagc atatcgtgag gtttctttat tattaaaaag
      481 acctccagga cgtgaggctt accctggtga tgtattctat ttacatagcc gtttattaga
      541 acgtgcagca aaagttatta ataacgattc ggttgcaaaa aacatgaacg atttaccaga
      601 aagcattaaa catttagtaa aaggtggagg tagcttaact gctttaccaa ttattgaaac
      661 acaggctggc gacgtatctg catacattcc aacaaacgta atttcaatta ccgatggaca
      721 aattttctta gaaggtaact tatttaatgc tggtattcgt cctgcaatta acgtaggtat
      781 ttctgtaagt cgtgtaggtg gtaatgcaca aattaaatca atgaaaaaag tagctggtac
      841 tttaaaatta gaccaggcgt tataccgtga gttagaagcc ttctctaaat ttggtggaga
      901 tttagatgca gctaccaaaa atgtaattga taaaggtgca agaaacgtgg agattttaaa
      961 acaaaatcag tacacaccat tctcggtaga aaagcaaatt gctattattt acttaggtac
     1021 acaaggtttg ttgaaagatg tagcagtaaa aaatgtaaaa gcttttgaag aaatgttttt
     1081 aactgaaatg caaaacaaat taccagaagt gttggctgaa tttaaaaaag gacaattaaa
     1141 taatgacagt attgataaaa tggtaaactt agcaaagggc ttaataccac aatataaata
     1201 attttaaatt tttatattct caaaatcggc ttcaataact tgaagccgat tttgtttatg
     1261 aaacaagttt tttaaaaaaa agaatatttt tactacttaa ttaataatat tttctataaa
     1321 taattacatt aatgaagcct tattggaata ttaaaataac tgttagggtt tatattttag
     1381 tactaacaat ttttactttt tttagaatta ttttgtttat aaatgaatat ggcagattag
     1441 atgaaactgc aactttaacc aataggttac tagctttttt tatgggtgta agatttgata
     1501 ttgttataag tggttatata ctatttttcc cttttgtagc tttatcaata ttaaatatta
     1561 caaaagatag cctacttgta aagagaatta ttttctattt tatttttata ttatttacgt
     1621 tggcattttt agtttgtgct gccgatattc cttattttaa tcaattcttt tctcgttttt
     1681 ctgtaacagc ttttgagtgg ttaaacagcc cgatgtttgt tttaaaaatg ataattcagg
     1741 agccacgtta ttggctattt tttattttgt ttgtggtagt ggtaattatt ttttttaaaa
     1801 ggcttcaaca tatttttaaa tcaaatgagt taaccattat taaaaataaa aaatggaaat
     1861 ttggattaat ttctttgctt gctgctggct ttattttttt aggtgttagg ggtaggatag
     1921 atgagaaatc tcctattaga gttggtacag cttatttctg taataatgct tttttaaatc
     1981 agttagggtt aaaccccaat tttactttaa tgagaagctg gattgatgca atggaagaag
     2041 aaaataaatt ggtgaactta atggatgaaa aaattgctat tcaaaacaca caaaaatatt
     2101 tgcaaataac taacccatcg caaacattac ccattagtag aatagaaaat gacacagtaa
     2161 ccaacaaaca taactataat gttgtattta ttattatgga aagcatgagt gctgcaaaaa
     2221 tgaaaagaca tggcaatgct aaaaacctca ctccattttt agatagcatt tctactaaag
     2281 gcttatattt tgaaaataat tactctgcag gcatacatac ttttaatgga atttttagca
     2341 cacttttttc ttatcctgca ttatttagac agcacccaat gaaagaaagt ggtatgttta
     2401 agtataatgg tattgcaagt actttaaaaa gtaataacta ttcaactatt tactttacta
     2461 cacacgatgg gcagtttgat aatgtagaag gttttttaaa tggaaatgat tatgaacaag
     2521 tcatttcaaa agaggattat ccttttggca aagcaccaac aacattaggt gtaccagatg
     2581 attatatgtt tgaattttct attcctttac taaataattt acaccaaaaa aataaaccat
     2641 tttttgctgc atttatgaca gcaagcaatc atcgtccttt ttatatacct gattattttt
     2701 cttcaaagtt taccgatgaa aaagataaaa ttgtagagta tgcagatttt gctttgagta
     2761 aatttattag tatggcatct catcaagagt ggttcaaaaa taccattttt gtttttgttg
     2821 cagatcatgg ttcgccaata agctctaagt atgatttatc tttagactat aatcatgtac
     2881 ccttactttt ttatgctcct gaaataatta caaagcataa aacatattca accatgtctg
     2941 gccaaattga tatttatcca acaacaatgg gcttacttgg cttaactttt accaataata
     3001 cattaggtgt taatttattt aaacaacaaa gaccatatat ttactttaat gcagatgata
     3061 agtatggagt aatagactct aattggttat taattgtacg taacgataaa tttaaaggac
     3121 tttataagta tagaaataat aatgttacca attatgccaa cgagtataaa gacattgtaa
     3181 acagaatgca aacctatgca gagtctaact tacaaacctt tcaatattta ttaaagaata
     3241 aaaagttata aatatttatt caaaaaatta aactagcagc actgcttcct ataaaagaca
     3301 ctacattcgt tacagaaaaa tttgttatga atattatagt aaataatatt gaatccctct
     3361 tttacaattg gcaaaaatct gcacctctaa aaattgaaaa attacaacaa agcggaagtg
     3421 atagaattta ttttagagtt tttggtagcc atcaaaatta tatagctact tataacttga
     3481 atattcaaga aaataaatcg tttattgcct ttgcaaggca ttttaaaaat aaaggtttaa
     3541 atgtaccaga aatttattgc cataatgaag attataccat ctatattcag caagatgttg
     3601 gcgaaaagtc gttattaaat ttattagaag agtacggaca aaatgatttt gtatttaatt
     3661 tttttaaaga aagtttaaaa cagttagcag cttttcaaat tataggcaat gaaggcatgg
     3721 attatagcct ttgccttact gcacaagagt ttggtaaaga agctatactt agcgatttgt
     3781 tgtattttaa gtattatttt ttagatactc taaagctacc ttacgataaa caagcaatgc
     3841 taaaagattt tgatgcattg agcacctact tgtcctattc ggaacataag tattttatgt
     3901 ttagagattt tcaaagcaga aatataatgc ttgaaaataa taaaatatcg tttatagact
     3961 ttcaaggtgg tatgaaaggt gctttacaat atgatgtagc atcgctttta tggcaagcaa
     4021 aagcagcatt aagcaatgaa tggaaagatg ctttattaga gtattatatg gatgaagcag
     4081 aaaaattatt acatcaaaaa attgaaagaa attcatttat tagccaatac catggttatg
     4141 ttttaataag gctattacag gttttaggtg cttatggctt tagaggtttg tttgaaagaa
     4201 aagcacattt tttaactagc attccacttg gattattgaa tcttaaatat tttattgaga
     4261 ataaaaaagt tggcattacc acacctgagt ttgacagagt tttaaaaata attatcagca
     4321 ataatatttt tgaaaaattt acaccccaac aagcaaatga tgaaacaaga ctgcttgtta
     4381 ccattaatag ttttagttat aaaaatggaa tacctgctga tacttcagaa aatggaggag
     4441 gctttgtttt tgatatgaga ggaattttaa accccggaag gcaagaagtg tataaatatt
     4501 tatctggcaa agacaaacct gtacaagatt ttttagacca gcaaactaaa atgcccaatt
     4561 ttttaaatag tgtttgggat ttaatagata taaatgtaga aaattattta acgagagatt
     4621 ttgaaaacct gatgataaat tttggttgca ctggtgggca acacagaagt gtgtatgctg
     4681 cagaacaaac tgcaagacat ttaagaaata agtataaact aaaagtaatt ctaaaccata
     4741 ccaaccaggc taattggtta agaaaataat tacacccaat gagtagtaca ctaaataaag
     4801 caatgttatt agcagcaggt ttaggcacaa ggttacaacc ctttaccaac catcatccca
     4861 aagcattggc tattgtaaac aataaaactt tactacaaag aaatattgaa tatttacaac
     4921 agtttggtat aagtaatgta ataattaatg tacaccattt tgccagccaa attacagatg
     4981 cgttgcaaca gaataatgga tggggtagta acattacaat tagcaatgaa agcaatgagg
     5041 tattagaaac tggtggaggc ttgcaaaaag ctgcttggta ttttgataac acggaacatt
     5101 ttttactgat gaatgtggat atacttacta atctgaattt atatgaacta ctgaatgctc
     5161 acattcaaaa taaagcctta gctactttag ctgttacaga tagggaatca tcgagatatt
     5221 tattgtttaa tgaaaacaat aaattatgtg gttggagaaa tgtaaaaaca ggagaagaga
     5281 aaataccagt accaactaat ccattatttc aaaaagcatt tagcggtatt caaataattc
     5341 aaactaaatt attaagccta atacagcaaa aaggtaagtt ctctatgata gatgtttatt
     5401 taagtttagc aaaagaaaat aatattcaat gttttaatca tagccataca aaattaatag
     5461 atgtaggcaa gccagagaat ttggaaaggg cagcaagcat gtttgagtaa aatttattgt
     5521 atcatttttt ctttttaccc ttaaacattt tttcgcctgc acgaatatta atttgcaaat
     5581 gattttctct taaaggtata gggcaattat agcccaaagc atatacacaa taagggttgt
     5641 aggctttgtt gaaatctaaa cttaaattgt tttgtttaat atcggctaat tcaaaatcta
     5701 aataccttcc accactataa ctttcattac cacaagtatt atctgtaaaa ggtaaaaaca
     5761 tgtatgcatt agtttggcta ctgtctttaa ttattttatt actatataaa gttaacctga
     5821 atggcttttt ataaagttta aaattaatta tagcatagcg ataaaatttt ttatcttttg
     5881 tgccagaagt tttcataata aaaccaatag tatcgctaag aagggtaaca gttgaattgg
     5941 ttttaaaatt ttcatcagct ttaaaaaaag aaaaaaactg tttatcatca ccaacaacaa
     6001 cttcatgttt agctacatac tcttgttggt attgtttgat gtactgctta tagttagttt
     6061 gagctattgc agaagaaaat actataaaca caaacaaaat aagttgtaga agttttttat
     6121 ttttcatttt tttaatcata ataaatataa aattaagcaa accgaataat ttttacacaa
     6181 taaccaaaac actaattact tttgttgcca taaaaaatta tatgaattaa tttttttcca
     6241 taatattatt agaacaaaaa ttataatgaa agtagcagta gtaggagcca ctggcttagt
     6301 aggaacaaaa atgttacagg tactggcaga aagaaatttt cctgtaacag aaattatacc
     6361 tgtagcaagc aagaaaagta ttggaaaaga aattgagttt aaaggcaaaa agtataaagt
     6421 agtaagtatg gaagatgcta taaaagcaaa acctgcttta gcacttttta gtgccggagg
     6481 aagcacaagt ttagagtggg caaacaagtt tgcagaagcc aatattacag ttattgataa
     6541 tagtagtgcc tggcgtatgg atgaaagtaa aaaattagtt gtgcccgaag taaatgcaac
     6601 tgtgttaact gttaatgata gaattattgc taaccctaat tgctctacaa tacaaatggt
     6661 agttgcatta cagccactac ataaaaaata taaaattaaa agagtagttg tttcaactta
     6721 tcaaagtgtt acaggtacag gtatgaaagc agttgaccaa ctttttaatg aaagaaataa
     6781 taaagagggt gctatggctt ataagtataa aatagatttg aatgctatac cacaaattga
     6841 tgttttttta gataatggtt atactaagga agaaatgaaa atggttaaag aaacttgtaa
     6901 aataatgcaa gatgaaacca taaaagttac agctacaacc gttcgtattc ctgttattgg
     6961 tggacatagt gaaagtgtga atgtagaatt tgataatgaa tttgaacttg aggaggttaa
     7021 agaaatttta accaattcac ccggtataat tgttcaacaa aatgatgcag aacaatttta
     7081 cccaatgcct atttgggcac acgaaaaaga tgaggttttt gttggtagaa ttagaagaga
     7141 tgaatctcaa cccaaaactt taaatctttg gatagtgagt gataatctta gaaaaggtgc
     7201 agctactaat gctattcaga ttgcagaata tttatataaa aatggatttt tgaattaata
     7261 tttatgctat tatttattca cccagataat cccaatccta gaaacataaa aacagttgta
     7321 gagtgtttaa aagatggtgg aactattatt tatccaacag atacaattta tggtttgggt
     7381 tgtagtattt atgaacataa agccatagaa aaagttgcca aaataaaaaa cgtttcgcct
     7441 gaaaaggctc atttaagttt tatttgttac gaccttagcg atttgagcaa atacactaaa
     7501 tcaatttcta caccattata tcggatgcta aaaaattact tgcccggtgc ttacactttt
     7561 atattacctg caagtaaaga agtgccaaaa attttacata gtaaaaaaaa gactattggc
     7621 ttgcgtgtgc cagataataa tattgccagg acaataataa aagaacttgg tagcccaatt
     7681 ctttcagcat cattacctgg cgaaatggtt gaagaatata ctgaccctga attaatgtat
     7741 gaaaacttta aacatttagt agatattgtt atagatggag gtgcaggagg aatgattcca
     7801 tcaacagttg tggattgctc acaagaacct tatcaattaa ttagaaaagg attagctgcc
     7861 tggaatgatt agctcctcat tccattttta acaacacagt tttattaaaa ttattctacc
     7921 aattttctgt tagtttcttt ttacaatttt ttgaataaaa ctttcattgc ctgctgtaaa
     7981 ccttaaaata taagtaccat ttggtaaatg ctgaattgga aggataattt gactattggc
     8041 atttgcgtaa tacttactta ttaaaatttt acctgccaaa tcggtaagtt gaatttgatg
     8101 cgacaaatta ttattaatgc ctgttttttc aataataata aaattcattg cagggttagg
     8161 ataaatagtg tatttactgt ttaaaggaat atgtatgcta aatacttcgc tgaattgaaa
     8221 acttccatca ttgtcaattt gcttcaatct ataataaacc cttctgcctc ttatattttc
     8281 ttcattgtcg gtaaaagaat aatcgttttt gttgttggct ggtacagttc caattgctgt
     8341 ataatctttt ccattaaaac ttctttctac attaaagcta ttaaagttta tagcattttc
     8401 tgtttgccat gttagaaata catttccatt ttttaattta cccgaaaaca atacaatatt
     8461 aataggcagt gcagctaagc cagaacatgt actattccac actcttgtta catattgcgg
     8521 actatcaata taaggatttc tatttccttg aaaattataa gtgccattgt ttctgtatct
     8581 ttctttttcg cttactggat cttgattatg ccatttaagc attagtttta agtagttgat
     8641 atttacacta ggaaaagtat cgttagataa tgttaaagct ccatctgtgt tatagccata
     8701 ccaagtagta attctatttt gatatcttgt aaccatatac aaataagctc tggcaaagtc
     8761 tcctttgtac atatctattg gttcaaaaac agtaccatta atgccagcag ttgcagagct
     8821 gcctagtttg ctaccattgc tgcttgtata ggatggagaa gccacttcgc caaaacgata
     8881 attagagcgt ttattattta cccatccatc tgttggaaaa aggtggttat aatctgtata
     8941 agcaggagct tcatcattaa accaacttgc aggaaaagaa tgttctctgt tataacaatc
     9001 ggcttcagaa ttataagatc cacattggtc tgttcctgga gtataataat aatctgcagt
     9061 gccatttgct ttaaaagagt acatatccca aataatatag ttattgccag aaggattttt
     9121 tatagtatct gtttttttgt actgatccca taaatcgcca taaccttgag ctgtatgtcc
     9181 attagtaata atggatttta aagttgattt taattctttg caatttttat tttttactga
     9241 atcgtaataa ccaactgggg cttgagcatt ggaagttata aaacaaaata atgccgaagc
     9301 taacaataat ctttttatca tctttttata attatttact aaccatttta attattggaa
     9361 caatcatttc ttctaaacta atacctccat gctgaaaagt atttttataa tagttcacaa
     9421 aatggttgta gttgtttggg taacataaat agccatcttc ttttgcaaat ataaatgatg
     9481 aattaacatt tggcacaggt aaacctgctt ctcttgggtc tttaaaagca agcacttctt
     9541 tagcttcata atgaaggttt cttccatgtt tgtatctaag gttagcagtt gtttgcttat
     9601 ctccaattac tttgtaaggt gtttttactc ttactgttcc atgatcggtt gcaacaataa
     9661 ggtttatttt tttgccactt atttttttta aagcctggtt tagtgcacta tgctcaaacc
     9721 agcttttggt aacactccta taactttttt catctcctgc taattctttt aaaacttcca
     9781 tttctgttct ggcatggctt agcatatcta caaaattgta cacaattata tttaagtcgt
     9841 tgtttaataa attatgtatg ttatttacta attgttgccc atcattatgg tgcaccactt
     9901 tagtataaga aacttttaaa tcatctttat ttaatttttt tagttgttgt ctaaaaaatt
     9961 tttcttcaaa taaattttta cctccttctt catcgtcgtt tttccattct aacggaaatt
    10021 ttttttctat atctattggc agtaatccgc tgaagatagc atttctactg tattgtgtag
    10081 cagttggtaa aatagaataa aaggtttctt cttctaaaat tctaaagttt tctgtaaaaa
    10141 ttggctgaat agctttccat tggtcgtatc gtaaattatc tattaaaata aaaaatgtag
    10201 cagtaccttt ttcaacatgt ggcaaaactt taaattgaaa taaattatgg ctcataatgg
    10261 gtgcatcggt tgttttggga tgcatccatt tggaataatt tttactgata aatttaaaaa
    10321 attccgtatt ggcttcttgt ttttgtgtag ctaatacttc ttgcatttcg gggctatcac
    10381 ttttctccat ttcaagctcc caataaacta attttttata aagttccatc cattcgttat
    10441 aatttggatt attgtttaag gccataaaca attctctaaa ctgttgttgg tagttggttg
    10501 tagttttttc agcaactaat cttttgttat caattatttt ttttaaactc aataaaacct
    10561 gattaggatt tacaggtttt agcaaataat ctgcaatttg gctacctatg gcttcatcca
    10621 ttagtgtttc tgtttcattt ttagttatca tcactactgg taattgctga tttatttctt
    10681 ttattttaga taaggtttca aggcctgtaa tgcctggcat gctttcatct aacaaaacaa
    10741 catcggtaat gttttctttt acatattcta tagcatcaaa cccattacta aaagtaatta
    10801 cctcgtagcc tttgttttct aaaaatattt tttgactttg taggctttcc atttcatcat
    10861 ctacccacat aattttagct aatggcatat tgtttatttt tacggaagat aaaagtaact
    10921 attaaatatt gttgttaaag ttgtttttat ataaatcttt tataaaaatg tagaggggag
    10981 ctgaaacaaa aaatctctgc attttttaat tattgcagag atttaaaatt attattaata
    11041 aacaaattag tgatgatggt ggtgcaactt gctagcaaaa tcttcggcac ttatactttc
    11101 ttctgttgca tttacttttt gtgctaaaat attaaataac ttttcagtac ttattctgtg
    11161 gtaactatct tctacaaatt ttttatcttt catcatttta tctgcataat cttctatcca
    11221 ttgttgttgt tcatctaaaa tacctaattg attacccata tattgaaata attgctgctt
    11281 agcaaaattt cttaaatctt ctggtagtac ttcaatttta ttctcttcag atagttgaga
    11341 ggtaattagc gtccatttta aactattttt aaaagaagga aattcttgtt cagcttcttc
    11401 gattgttttt ggagtttcgc tgtttgtttg aatccaacgt tttaaaaatg cttcaggaaa
    11461 atctatagaa gtattatcta ataattgatg ataaatttgg tcgtgaattt gattgttgct
    11521 ttgttgagta aagtaggctt ctatatcttc ttttacagca ttattaaatt cttcttctgt
    11581 ttttatttcc ttgttcgggt atgcagctat aaaaagttca ttattgattg ctgctttttc
    11641 aactaatcct attttagtaa ttgtaagttt aaagttttta tctgcaactc cagcaatatt
    11701 tttatctaat cctaaatcgc ttaatattac atctaattct ttatcttcaa aagctgttgc
    11761 taaatgaaca tcaataacat cttctaattt tttacctaat aaattggcac gaaaagcagg
    11821 agcaaaatat ttaactaaaa cactattcgg tttattaata ccattttcta ccatgttacc
    11881 ttctttatct acttctgtaa acgttacatt taacatagtt tcgtcatcag taactgttgc
    11941 tggctctgtc attttaccaa atcttctttg taaacgatct atttcatcgt ttaacatttc
    12001 gttggttaca tttattttat acctggttac attaaaatta gtattaatgg tatagcttgg
    12061 ttttagccct atttcaaaag caaaagcata atccatagga ttattcatat ctaatgctct
    12121 agcatcgcta tctaggggta aaggctgtgc aaaaatttct aatttctctt ttgtaagata
    12181 ttcgtttaac tgtgtttcta cagtacgcaa aacttcatcg gtaaatacgc tttggccata
    12241 cattttttta actaaacctg ctggtaccat accttttcta aagccaggta tattagctgt
    12301 tttagcatat ttctttaagc ttgtttcaaa gttttttaaa taatcttctt tgctaagatt
    12361 tactgtaagt ttatcggtaa ggttaccaat attttctctg gttactgttg ccatagtaag
    12421 ggcctgttgt atgtttttaa aataaagaag ttgataagaa aagagttgaa gagaattgtt
    12481 caacccctca acttatcaac ttcacattag tgcgggtgga gggactcgaa cccccatgcc
    12541 tcacggcacc agatcctaag tctggcgtgt ctaccaattt caccacaccc gcaggtgtat
    12601 aaatttaggg tggcaaaagt agggttttta ttgtaaaatt tcacaattgt atcatttagt
    12661 ttaaaagttt tttatggtta ctattttgct aaaaaagtaa taccaacata ttgtgaatat
    12721 aaaaaaggta atattttatt gtatttatca gaaaaactgt agtttgtgca tcgctaataa
    12781 gttatctaac ccaaaaatga ataattatgg acaaaagcaa aatgattaaa gcctattgcc
    12841 ttaaaacaaa agaaaaaaat gtaccaatgc atgatgctgt tataagtaaa acatcaagag
    12901 gtggttacat ggcatctggc aatgatggta aaggcaataa aatgtcggca atgctaggcg
    12961 aagcaaaagc tttacaggca attgcagatg gtgttgctaa aaaaggtttt taatagcttt
    13021 taattttttt atgttacagt attaaagtga acaactttaa tactgtattt tttatagaga
    13081 taggtctata attgttttgg ggtataaaag atgtaaggtt tgtaagcctg caagttttgc
    13141 tccttctata ttaactaaag tgtcatctat aaaaagtgtt tccttaggtt gcaggttttg
    13201 ttcttctact atttttaaat agctttcttt ataaggtttt ctaaaaccac aaatatgaga
    13261 gtaatatgct ttaataaata gctcatcaaa atttttagat ggagtggcgt tattggctaa
    13321 atctataaag cagttataat gaatttggtt ggtattggag tataaaaaaa tattgtactt
    13381 ggtttttatt tctagtagcc aattaattct ttcttttggt aaattaagca acatggcatt
    13441 ccatgcagtt tgtatttgaa gattagataa agagtttttg gtaatttttc tgaatgtatt
    13501 ataaaattct tcggggctaa tatttcctgt ttctaattgt tcaaataaca aagaagattt
    13561 atgttgatta taaaacgctg aaaaattagt tataccaagt tgtataaaag ctttttcggt
    13621 taataaataa tctatgttta aaattacact acccaaatca aaaatgatat ttttgatatt
    13681 acccataaaa aaagttatta atagttgcaa tattaataac tcttttatct gtattttaaa
    13741 ttaattatct tgctggcata tttaaaggaa tatctctttt tagcatatca tttctattag
    13801 ctatttccca tgcagtggca aaaatgtacc tggctctttt agtcattaca tcaaaattta
    13861 ttttatcaac tgtatcactt ggtctgtggt aatctttatg tactccatta aaataaaaaa
    13921 taacaggtac tccatttttt gcaaaattgt aatgatcgct tctgtaataa aatctgttag
    13981 gatcttttgg gtcattgtat tttctatcta attctaattt atacaatttg tttacattat
    14041 tgctaatagg tgttaaatcg ctacttaact tatcatctcc aataatataa acataattta
    14101 agctatcgcc tttataggtt gggtctattc tacctatcat atcaatattt aggtttacag
    14161 tagttttggc taaaggataa attgggtgtt gtgtgtaata atcgctgccc cataaacctt
    14221 tttcttctcc gcttacagtc ataaaaacaa tacttcttct tggtgcatat ccttttttct
    14281 ttgccttaat aaaagcttct gccatttcaa taatagtaac tgttccactt ccgtcatcat
    14341 cggctccata ataaataaca tctccttttt tacctaagtg gtcgtaatgt gcagttaata
    14401 caacaaactc ttctttttta tctgttcctt ctaaaaagcc aactacattg ctactttcaa
    14461 gtgtttttgt ttctttatta ttatttaaaa ctatttgtgt agtataagta cctgtttcaa
    14521 tattttttaa atcgctaaaa ttaactatgg tttttgttag aatagatgct gctgcctgat
    14581 acgaaattga gataataggt gtagaaagat tattgctttg tttttttaca tacatactac
    14641 cagtaattgg tagtgtacta tattttggat tatcaaagtt tttagaaact aataaaacac
    14701 ctatagcacc tttgtctaaa gcggttttaa tttttgtaaa actttttctg gaatctgctc
    14761 cagatttttc tttgtcaata tcttcggggt tactttctat tatcattacc cattttcctt
    14821 ttatatcaat attttcataa ttgttcattg ttttatcagt aaggccatag cttgcaaaaa
    14881 caatttgatt aattgaaatg ctaccattgc ttgtactatt tggttgaatg gtataatgat
    14941 tatttaaact aaaatgtgca tcgtatattt ttagtgaaga actgtgtaat acatcttgat
    15001 atactttaaa gtattggtag aatgaattag cattaccagg ctttaagcct aaatttttaa
    15061 attgctcttc aatatataat gcagcttttc tttgcccaac tgttgctgtt tctcttcctt
    15121 cagtttcatt gccagcaatg taagtaaggt gttgtttcaa atctttttga gtaattgtgt
    15181 ttgcaaattt ttccacattg tagttggttt gtgcaaatgt tgcagcaaca ataaaatagc
    15241 aaagtgtagt taaaacaatt ttcattgtag tttatttaag gtctgaataa taaaaggcaa
    15301 aatagtgttt tacagattat tatacaactg aaaattgtga atggtaaaga tatgagtagt
    15361 tttaacttat taaggtagtt aagttaggaa gcaatagctt ctgtaaattc taaaactctt
    15421 tttatttcct gaaataatgt ttcggaagaa aatggtttag aaatatagcc attcatgcca
    15481 agtgctaagc atttttcttc ttcgcctttc attgctaagg ctgtcattgc tataattggt
    15541 atagaagaat gaagttgatt gcgaataata tctgttgttt gatagccatc taattctggc
    15601 atataaatat ccattaaaat agcatcaaat ttattttggt tcattagttc taatgcttgg
    15661 ctgccgtttt ctgcaacgat ggtgtttgca ccagattttg caaaagcata ttttaccaac
    15721 atttgattaa ttgcattatc ctctacaatc aatatatttt ttcctattag taattcattg
    15781 gtcataattc aaaagcattg gtttacaaag aaaatcggat ttatattgtt ataacgataa
    15841 aaaagttaat tcattatccc aaaagggtaa aataatttaa ttttttataa atatttaacc
    15901 aataaagtga taccatttct attatttttg ctgctttagt gctttttaaa gcctatttaa
    15961 ttaaagttta tgaagaaaat atttttgtta ttttttatta caattgggtt tacaagttat
    16021 gtatgcagcc aacaagtaga aaaagcttcg aagaaaaaat atgatttaac taatcgcact
    16081 tccgatcatt ttatgttaca atatggaatg gatagatggt ctggtgcacc agatagtttg
    16141 aatgtaaaag gaagcagcag gcattttaat ttttatttta tgcttgataa gccatttaaa
    16201 aacagtccta aatttagtgt ggcttatggt gttgggatag gcagtagtaa tattttttta
    16261 gataagagat atgccgatat aaaatctaat acaacagtat taccttttaa agttagctac
    16321 ccaggtacag attcatctta ctttaataaa tttaagatga ctacaatttt tttagaagca
    16381 cctgttgaat taagatttta ttcagaccca gaaaatacca ataaatcttt taaagctgct
    16441 ttgggggtaa aagttggtac tttattaaag gcatattcaa aaggcaagga tttaaagaat
    16501 aaatctggcc aatctatttt tggaaatagg tttatagaaa aacagtacga taaacatttt
    16561 tttaatggaa caaggcttgc ttttacagca agaattggtt acggaatttt tggcttacat
    16621 tttagttacc aaataactca agctattaaa gaaggggcag gtgctgaagt taagccttat
    16681 agcattggac ttactattgg cggattgtaa ttattaaatt taaattattt taaaggtggt
    16741 attttaaaaa atactgcctt tttttagtag aatttttttg gggtttgccc tcttacaaag
    16801 aacatttttc gtgatagttt atcaactcta ttggcgtttt ttcaatttca aatccattgt
    16861 agtagttgta tatctcctct aaagtttgta taatttcttc tacttctatc atggttacaa
    16921 gtttacctct aaactcttta atatttggta agccttttaa atagttggta taatgtcttc
    16981 gcatttcaaa aatgccaact ttatttcctt tccattcaac cgattttaaa agatgttttt
    17041 tgcaaacatt tattctttct aaaagtgtag gaggtgaaag ctcttggcct gtttgtaaaa
    17101 aatattttat ttctctaaaa atccaagggt agccaatagc agctctgcca atcattatac
    17161 catctacacc atatctttct ttatattgtt tagctttttg tgggctatca atatcgccat
    17221 tgccaaaaat aggaatattg attcttggat tattttttac ctttcctatc aaagtccaat
    17281 ctgcctcgcc tttatacatt tgacacctag ttctgccatg tatagccaag gcttttatac
    17341 caacatcttg taatttttcg gctacttctt caatgttttt tgtatcgtcg ttccagccta
    17401 atcttgtttt tacagttacg ggtagtttgg tgctttttac aacagcctgt gtaagtttaa
    17461 ccatcaaatc aacatctttt aatacgcctg caccagcacc ttttgtaaca acttttttta
    17521 ccgggcatcc aaaattaata tccaccaaat ctggattaac tgtatctaca attttggcac
    17581 ttaatgctaa tgcttcttca tcgcctccaa aaatttgaat accaattggg cgttcataat
    17641 caaaaatgtc taattttctt ttactcttta ttgcatgcct tattaaacct tcactgctaa
    17701 taaattcagt gtacatcaag tctgcaccat tatctttaca aatggctcta aatgggggat
    17761 cacttacatc ttccataggt gcaagtagaa gagggaagtc gttcagatta atattatctc
    17821 caatttttac cattactatt ttattttttt aaaagcaggg tgcaaaatta gctacaatta
    17881 atcaattgaa attttcttta acctataatt tactagtgct ataaaaaaaa ttacctagtc
    17941 tccattttta ttttcctctt cttcattgtt tttcttgcta tcttttttct tttttttctt
    18001 tttgtctttt ttggtctctg ttttatcttc ttctttcttt tgctctgctt tatcttcttt
    18061 cttttctgtt tctgctttta cctcttttgc aggttctgt
//
LOCUS       DAOQPK010000020        17078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_1681_length_17078_cov_4.709099, whole genome shotgun sequence.
ACCESSION   DAOQPK010000020 DAOQPK010000000
VERSION     DAOQPK010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 17078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17078
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1681_length_17078_cov_4.709099"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="PLU36_01885"
     CDS             complement(<1..668)
                     /locus_tag="PLU36_01885"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HMZ45530.1"
                     /translation="MYKLIRDILFCFPPENVHYFSMNMLKMLCSNKAIKNLISNQFQF
                     QQTTLQKEVFGLQFKNPVGLGAGFDKNALYLNELNALGFGFVEIGTVTPLAQNGNDRP
                     RLFRLPKDKALINRMGFNNDGVHEIKKRLIHWRNQPSLQSNQLIIGGNIGKNKITPNE
                     NAWKDYEICFNELFELVDYFVVNVSSPNTPGLRELQEKDALRKILAHIQNQNQGKTMP
                     KPLL"
     gene            complement(702..1814)
                     /locus_tag="PLU36_01890"
     CDS             complement(702..1814)
                     /locus_tag="PLU36_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ45531.1"
                     /translation="MSFTTKILVIEDEDNIRENIQELLEAKQYLVRTANNGRQGVLEA
                     IDFRPHLILCDIMMPKMDGYKVLEYIRKTSIIQNVPFIFLTAKIDRNDIRQGMELGAD
                     DYLTKPFTAKDLVTAIETRLKKQQKIIDEYAKVKHELDTSAFASYYHEFNTPLHGIIG
                     GINLLLSSRTSFNDTQIQELLYSILKSGLRLNHSLANLMLFEEIKRAEVHTELLSMFN
                     SGNSSGKWADRIKTELEVIAREIYNRPEDLVFDMQATDVHISPEYLMRILLELTDNAL
                     KFSSKGSKISIKGKPDGDNYFIEIKDSGKGFAIENNNQIAPFKQFNRSKYEQQGLGIG
                     LYLVKRLVEINSGEIKIESEENKGTTITILLPSFGI"
     gene            complement(1827..4628)
                     /locus_tag="PLU36_01895"
     CDS             complement(1827..4628)
                     /locus_tag="PLU36_01895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ45532.1"
                     /translation="MPVTESIETKNNQLLAQCYEQLINNNINDALSNITNLIIQYTNA
                     AMCCISVNRDKANKLYHCANNDQIIFSENKVQQFFKQFKEIQPTHSSFNLRSNSNENI
                     SLFANSPIKQILGYSFNHNNNELILLAFKEPVEENIYSSILQKLSTAISKVASTTTIQ
                     EELFFRNDVYESTLYTLNQIIWDHNLKTDTTHVMGFAHRIGNFIPQKTFQYNIKDWLL
                     DKAHPDDMQRVMQNYEQFILQKNNTVNEIKFRMFSPTANEYFWVQTRQTLLYDETGEP
                     ALVVGTTIDIQDTYQIAADLEKHKIQNLLLVESLQESNEKLLTILNSSKEIIFTIDLQ
                     TSQFENVNKAISLLGYTPKEWIGQNYTQWSLDKRKKFHNLLKHASASNNSVKTQQISF
                     TTKDEKTEIPFEFSTSVFTFKNKKYLLCILRDISERVAYENSIKQVTEQLAYLINNID
                     DVYAIYNLQQKKYEFVSNNIEYLYGCSKDTYLSNDLYWKEIIFKDDYASIESQMNDII
                     SNKSKGEFFYRINTPVGETKMLLEKVTVTTDKDGNADKLFIVKSDYTNLENAEQNLIE
                     SERKFRFISENITDFISIIDIDGRFMYASPSSVKVIGYTTEELIGKNIIFFIHAEDVT
                     NFLEEALEKIVFNKEEASLRYRLNKKDGSFNWVETYFKPIIDANNDTTSIICSTRDVT
                     EREQLMKELQQSLNKERALNELRAQFVSMASHQFRTPLTVIQSGIELMQIYLEGISEQ
                     KQQPFKKQFLRIESEVSRLQDLMNDVLLLGRAEANRTPYNPTFGNIIQFCRDIIESRY
                     NNQDYNNRKVILYIFGTAQEVMFDAKLLDHTLENILSNAFKYSNKGDIKFTITFSETT
                     VNIAVKDQGIGIPANEIENLFQPFYRATNATEFEGTGLGLSIVNEFIETHGGKLFVES
                     EIDKGTTVNIQLPLYQIKK"
     gene            complement(4746..6002)
                     /locus_tag="PLU36_01900"
     CDS             complement(4746..6002)
                     /locus_tag="PLU36_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HMZ45533.1"
                     /translation="MEEVYNLIWLFCSIIIIGFFAGYEIAFVSTNKLSVELKKKQGKK
                     SGIVIAKFLEKPAEFIGSCLIGLNFFYVIYGLAFNQFLQGSFWSFFHVKNGYLQLAIN
                     TLIAGIVILVLGEFFPKAIFRAKNDMLISVFAPLANIFHSLFAPIARFFVSVSQWVLK
                     YLFNIKMSEKEEAFSHIDLEHFFQQAKEHDESGEELNKELFENALSLPTVKIRQCLVP
                     RTEIEGIDIESNIEEVKKIFIQTKLTRLIVFKDNIDNILGYIHQLDLFKKPKNIQDVL
                     LPIPVIPEAMNAIDLISKFTKERRNIAWVVDEFGGTAGIVTLEDILEEIFGEIHDEYD
                     TDDFVAKQLSENEFVLSGRLELDMLAEKYKLTFDVDDSETLSGYIINFYGSIPNQRES
                     IIIGNYQFDILSVSDTRIENVKLKVL"
     gene            complement(6083..7468)
                     /locus_tag="PLU36_01905"
     CDS             complement(6083..7468)
                     /locus_tag="PLU36_01905"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HMZ45534.1"
                     /translation="MKNKPSFIVLTKISPKALFNAAKFFVAIAFVLVASFQLHAQTKE
                     ELQKKEDELRKEINELSKNLVQIQKNKKVSLAQVASVQRKIAAREELIRTINRRVRLL
                     DETIYQNELEIYRMRKELDTLKQQYARSIAFAYKNRGNYQYLNFLFSSTSFNDALKRV
                     TYLKSYRKLRETQVGNIVKTQQVLQQKIGTLTNSKNEQNQVLESQKAQLVDLEADKKE
                     KNDVVKGLKGQEKEVTALLRKKEKQRAEMRNTIAAIIRREIAEAEKARLAKLKAETDA
                     RNKANAANKNIAKTTTTKPGVATTEVVEEMASTKTVNREYSALESTAEGVEMSVNFEK
                     RNLNWPVSIGTVIAPFGNYQITEKLQGHSDGIEIAVPQGTAVKSVADGEVTYVGDFGG
                     SPAVIVKHGKYFTIYNYLSEITARKSQQVKAGTVLGKSGISTFGEESLLFMVTNDKTV
                     FLNPRNWLKSR"
     gene            complement(7634..8467)
                     /locus_tag="PLU36_01910"
     CDS             complement(7634..8467)
                     /locus_tag="PLU36_01910"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HMZ45535.1"
                     /translation="MNKQLLVGCTLLLVVIFSCRTTKNTHNIQTAITKKDTTQVVTVA
                     EEKSIDSFAVVKEILNQTINHKINFSTFYAKIKVDYESPSDSRKLTAYTYMQKDSVIY
                     IRLVGSFLGITKEGVVAKITKDSVTVINKIDKIVQKRTIAYLQEVTQIPFDFSTIQDV
                     IIGNPVYIDSNIVSYRNSLDDFSVLMLGNLFKHLITLDKNDFKVLHSKLDDIDVFKNR
                     TCNITYSDYVDAFGVKFSTKRKFSVSEKTKLDVWLDFKQYTFNEPLTYMFNIPKNYKK
                     Q"
     gene            complement(8477..8911)
                     /gene="dut"
                     /locus_tag="PLU36_01915"
     CDS             complement(8477..8911)
                     /gene="dut"
                     /locus_tag="PLU36_01915"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008629012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HMZ45536.1"
                     /translation="MQINIINKSTNPLPTYETKGASGMDVRANINEAITLQPLQRALI
                     PTGIFIEIPQGYEAQIRPRSGLAIKQGITCLNTPGTIDADYRGEIKIILINLSNETVT
                     IQHGERIAQMVFCKIEQAELVQVQIINETTRGTGGFGHTGKQ"
     gene            complement(8916..9581)
                     /locus_tag="PLU36_01920"
     CDS             complement(8916..9581)
                     /locus_tag="PLU36_01920"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HMZ45537.1"
                     /translation="MSKNNFSFGFNQNSFTNEQLQYKALQINGSLAKRFFDIAVSICF
                     LLFIFSWLYPIIAIAIKLETKGLAMFVQDRIGLNGKIFKCYKFRTLLSNSNSLDKDSL
                     PLSYTTPATKVGVFLRKYNLDELPQFINVLKGNMSIIGPRPHAVPFHNLYSEYVPNIH
                     QRLLVKPGITGLSQINGLRGDVEDIEQNKKNIQQRVQCDTNYIFHWSFKLDLKIFVKT
                     FIG"
     gene            9673..10218
                     /locus_tag="PLU36_01925"
     CDS             9673..10218
                     /locus_tag="PLU36_01925"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase/exosortase family protein"
                     /protein_id="HMZ45538.1"
                     /translation="MANSFFQTIPSAVKKFLLRSIVFFIIWKLIYHLVLYPIRVPDKQ
                     LTHITAVITKEMIQFHYPNDKLNIEEINYPSPKENLLKNNKKIVGIADGCNGLELYIL
                     YIGFLACFPSNKKRLFAYAITGIVLIFVLNNIRSYVITLLNIHNSSFTDVAHHYFFKI
                     FIYAVMFLLWVKYTKPKLKDE"
     gene            10211..10612
                     /locus_tag="PLU36_01930"
     CDS             10211..10612
                     /locus_tag="PLU36_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45539.1"
                     /translation="MNKTFKHIIAISLILIVCWWGYTNTEEINKISNVHKPFEQQLKR
                     IIWLLIVGIITWWAFAKDNNQWLSKIVIIIYAFVIITIGLIGVLEWKFKFLSENWREF
                     FAGVRLFLSSPLPFIFLWIMRKINFDHFSKN"
     gene            complement(10609..12195)
                     /locus_tag="PLU36_01935"
     CDS             complement(10609..12195)
                     /locus_tag="PLU36_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45540.1"
                     /translation="MVICKFNVAIGQAKFPVKIQNKLYAQLYEKWARENNIYSIAALQ
                     PYCNTDIRFNKELLSFYQLPSDSLRKKRNFFTQTFRNGNMAEFVSDKSTVTINPLFQF
                     EFGKSKDYSVYHFNRGIQLDANFNNKVFISSSIYENVSKFPKYINQYIDSLTVVPGMG
                     KARYNTNGKNIEYALPIGFIGYKPNKNFYFELGNNKNFIGNGYRSLLLSDVAYSYPYF
                     KMQTHISKFTFQTIWSQFVDASKNWVNTNGYDKKFGAFNTVSYTGIKNVELNLFQSVI
                     WSNKDSLGNRRDQEWGYFVPIIFFNSLNFNNGSPDNSLIGVDASYTIKKHTVIYGQLI
                     IDDFNIRESKNGKGFFQNKYGVQLGVKSFKPFNLDNAFARIEFNTVRPYTYASKTPTL
                     SYTHYGEVLAHPFGANFREFLLEANYKIKRYLFSANLIIAWYGADDANSHWGKNIYKS
                     DYLSQTGIFSFNNKITQGIKTNLIYSNVAIRYLMNPVTNSGIAAQIFYRTEKSNLINN
                     KDFIFNISYSTNLLNILKEF"
     gene            complement(12230..13624)
                     /locus_tag="PLU36_01940"
     CDS             complement(12230..13624)
                     /locus_tag="PLU36_01940"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR03023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HMZ45541.1"
                     /translation="MHHKYAAFIRFLNFIYDLAILNIILLFLFLRFSPSVINVSDLLF
                     NKVYFFNWLNINFAWIAATIFTNVYTLRNLIYFKEGVKNSFNGLAIFILFVVCINFFL
                     SSVVFFSKKDLLIFIILFSTFFWLARLVFYYFKKRYNLELYAGKRILLIGDESAIDEV
                     KKTFYEKKELGFVYAGHLPDSEFHANNQKYIENLLASIQLQNIDEVFFAKSSIKDEEL
                     YNLIQTLDKNTIRVRVIPDFFRFYTKFKNLSFHGNLPLLSLREEPLQNILNRLLKRFF
                     DFIFSLLVIILLASWLFPLLALLIKLESRGPVFFKQLRSGKDNKQFWCYKFRSMRVNV
                     NADVIQATKGDQRITRIGKFLRKTSIDELPQFLNVFIGNMSVVGPRPHMLKHTEQYAA
                     MVDKYMIRHFVKPGITGWAQVHGYRGEISNVEDIEKRVEYDIWYIENWSLALDIQVVL
                     LTVYNIIRGEENAH"
     gene            complement(13632..14369)
                     /locus_tag="PLU36_01945"
     CDS             complement(13632..14369)
                     /locus_tag="PLU36_01945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HMZ45542.1"
                     /translation="MRIELFNCPIDAITMHQTLNKIENAIIQKQHLHHVVVNVAKLVH
                     MQNNSELRESVVNCDIINADGQGVVWASKILGKKIPERVAGIDLMQNLVQLAHDKKYK
                     IFFFGAEEHIVKEVVNKYTNQFSHSIIAGYRNGYFNKEEESEIANEIANSGANILFVA
                     MGSPKKENFLFNYKQVLQKLNFVMGVGGSFDVVAGKVKRAPVWVQKIGMEWFYRMAQE
                     PRRLFMRYFTTNLALIKLILKEKLKKG"
     gene            complement(14350..15528)
                     /locus_tag="PLU36_01950"
     CDS             complement(14350..15528)
                     /locus_tag="PLU36_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45543.1"
                     /translation="MNSNFENSFSKLQQYVEEENYKGWDPYDGLNSKVFQAIPFIKNN
                     RFCRLVWIQGFKKSPINFRKLLLVEKGYNSKGIALFLSAYCRLYTIEPTEKNVQKIKE
                     LSTKLLEMKADGWSGYCWGYNFDWQARAFFQPKNTPTVVASVYASWALLEAYEILQDK
                     KLLDAAESCCSFILKDLNRNYDADGDFCFSYSPLDKSIVFNASLLGSRLLSKMYSLTK
                     NELYKIEAKKSVSYCVKHQQSNGAWAYGLQSFHYWIDNFHTGFNLECIAAYQKYTGDT
                     SFETAISKGFEYYINNFFEEDGKPKYYNNKTYPIDIHAPAQLVITLYELGLIQKEEKL
                     CSTVLNWTLQNMQDNNGYFYYQINQSLNSKIPYIRWAQAWMFFALATYKKAFHENRIV
                     "
     gene            complement(15545..>17078)
                     /locus_tag="PLU36_01955"
     CDS             complement(15545..>17078)
                     /locus_tag="PLU36_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45544.1"
                     /translation="AATPIADSVIVSAAYWSAKQEQLSGNGYVKINGSNISYDSSTFI
                     TIQDSTEQNASFKISGNATANSFKINSTNSLGLISKNLAGVNIQQINAGNDTTNINSY
                     NSFIGHNLWFDGFSFIRRDRRIKGNGVLLNGNTMTLVQDTFNASTPDNNYPVSVVYAP
                     IYNSFNIESIGLIKQDFLGNGALQIIPQNTNINSGMAGLRMNKNSSLHHNPHGFMFSV
                     DDITKWDLGMDYENEDFVLAYNHHTNHKGDFIRIAENNRMVIGTGIGTPKYNLFKYTF
                     STSSSDSIGLGGIKIQTYSNLDTALNTNGVFAGSKAYFTSHIKSDDYIQAGMYNGKAR
                     LTSYFNNTNFAQFVNTQMASSITNYGFMQNNTGELFVGGTKLNVNTSAYFTNDIFSNN
                     KRVLTTEDSVKFLIRTVEFNNVSTLNYTLTLSDATKMININQIDINTITIPNQLSVAF
                     PIGTKILVTQMGVGQITFMADGGVTLNSSSNANKTKNQYSVATLIKTDTNTWLLFGDI
                     IN"
BASE COUNT         5615 a         2699 c         2250 g         6514 t
ORIGIN      
        1 aagagtaatg gcttaggcat tgttttgcct tgattttgat tttgaatatg tgccaaaatt
       61 tttcttagag catctttttc ttgcaattct cttaaacctg gtgtgtttgg gctacttaca
      121 ttaactacaa agtaatcaac caattcaaat aattcattga aacaaatttc ataatctttc
      181 caagcatttt cgttgggtgt aattttattt ttaccaatat ttccgccaat aattaattgg
      241 ttactttgaa ggctaggctg gttacgccaa tgaattaatc ttttttttat ttcatgaaca
      301 ccatcattat taaaacccat tctatttatt aaagccttgt ctttgggcaa acgaaataaa
      361 cgtggccgat cgttgccatt ttgagctaat ggtgttacgg taccaatttc tacaaatcca
      421 aaaccaagtg catttaattc gtttaagtaa agtgcatttt tatcgaaacc tgcacctaag
      481 ccaacaggat ttttaaattg caacccaaaa acttcttttt gtaaggttgt ttgttgaaat
      541 tgaaattggt tactgattaa gtttttaatt gccttgttag aacaaagcat ttttagcata
      601 ttcattgaaa aataatgcac attttccgga ggaaaacaaa acaaaatatc acgaattagc
      661 ttatacataa aacaaagata aaggcgaaag ttacagctaa gttaaatacc aaaacttggc
      721 aataaaatag ttatagtggt tcctttattt tcttctgatt ctattttaat ttctccacta
      781 ttaatttcaa ctaatctttt aaccaaatac aagccaatgc ctaagccttg ttgttcatat
      841 ttagaacgat tgaattgctt aaaaggtgct atttgattgt tattttcaat agcaaaccct
      901 ttaccactgt cttttatttc aataaaataa ttatctccat ctggttttcc ttttatagaa
      961 attttgcttc cctttgatga aaattttaat gcattatctg ttagttctaa taaaattctc
     1021 attaaatatt caggactaat atgcacatcg gtggcttgca tatcaaaaac taaatcttct
     1081 ggtctattat aaatttctct tgcaataact tctagttctg ttttaattct atctgcccat
     1141 ttacctgacg aattaccaga gttaaacatg cttagcaatt cagtatgcac ttctgctctt
     1201 tttatttctt caaaaagcat taggttggct aatgaatggt ttagccttaa cccagatttt
     1261 aaaattgaat aaagtagttc ttgaatttga gtatcgttaa aagaagttct ggaacttaat
     1321 aataaattaa tgcctccaat tatgccatgt aatggtgtat taaactcatg ataatagctt
     1381 gcaaaagcag aggtatctaa ttcgtgtttt acttttgcat attcatcaat tattttttgt
     1441 tgttttttta atcttgtttc aattgctgtt actaaatctt tagcagtaaa tggtttggtt
     1501 aggtaatcat ctgctcctaa ttccattcct tgccttatat catttctatc aattttagca
     1561 gttaaaaata taaatggaac attttgaata atactagttt tacgaatgta ctctaacact
     1621 ttataaccat ccattttagg catcattata tcgcataaaa ttaagtgtgg cctaaaatct
     1681 attgcttcta aaacgccctg tcttccgtta tttgctgtgc gtacaagata ttgtttagct
     1741 tctaacaatt cttgaatatt ttctcttata ttatcttcat cttctatcac taaaatttta
     1801 gttgtaaaac tcataaaata ttggttttat ttttttattt gatataatgg aagttgaata
     1861 tttacagtgg tgcctttatc tatttcactt tccacaaaca atttacctcc atgtgtttct
     1921 ataaattcat ttacaatgga taggcctaaa cctgtacctt caaattctgt tgcatttgtg
     1981 gcacgataaa aaggttgaaa taaattttca atttcgtttg caggtatgcc aatgccttgg
     2041 tcttttacag caatatttac tgttgtttca ctaaaagtaa tagtaaattt tatatctccc
     2101 ttgttagaat atttgaaagc attgcttaaa atattttcta aagtatggtc taataattta
     2161 gcatcaaaca ttacttcttg agcagttccg aaaatatata atattacttt tctgttgtta
     2221 taatcctggt tattatacct actttctatt atgtctctac aaaattgaat gatgttacca
     2281 aaagtagggt tataaggtgt tctattggcc tctgccctgc ctaaaagtaa tacgtcattc
     2341 attaaatctt gtagtcttga aacttcagat tctattctta aaaattgttt tttaaatggc
     2401 tgttgttttt gttcagaaat gccttctaaa taaatttgca ttaattcaat accgctttga
     2461 attactgtta gtggcgtacg aaattggtga gatgccattg aaacaaactg agcccttaat
     2521 tcgtttaatg ctctctcttt atttaaagat tgttgcaact ctttcattaa ctgttctctt
     2581 tcagtaacat ctctagtgct gcaaattata gatgtagtat cgttgtttgc atctataatt
     2641 ggtttgaaat aagtttctac ccaattaaaa ctgccatctt ttttatttaa cctatatctt
     2701 aaacttgcct cttctttatt aaatactatt ttttctaaag cttcttctaa aaagttggta
     2761 acatcttcgg catgaataaa gaaaataata ttttttccaa ttaactcttc tgtagtgtag
     2821 cctataactt ttacagaaga tggagatgca tacataaacc taccatcaat atcaattata
     2881 gaaataaaat cagtgatgtt ttctgaaata aatctaaact tacgttcact ttcaattaaa
     2941 ttttgttccg cattttctaa attggtataa tcagatttta caataaacaa tttatcggca
     3001 ttgccatctt tatctgttgt tacagttacc ttttctaaca acatttttgt ttctccaact
     3061 ggtgtattaa ttctataaaa aaattcgcct ttacttttat tgctaattat atcgttcatt
     3121 tggctttcta tactggcata atcatcctta aaaataattt ctttccagta taaatcgttg
     3181 gagagataag tatctttact acagccatat aaatattcaa tattattgct tacaaattca
     3241 tatttttttt gttgtaaatt ataaatagca tacacatcat ctatattatt aataaggtat
     3301 gcaagctgtt cggttacttg ttttatacta ttttcgtatg ctactctttc agagatatct
     3361 cttaaaatac agagtaagta ttttttattt ttgaaagtaa aaacagaggt agaaaactcg
     3421 aaaggaattt ctgttttctc atcttttgtt gtaaaactaa tttgctgtgt tttaactgaa
     3481 ttattgcttg cactggcatg ctttaataag ttatgaaatt tttttctttt atctaaagac
     3541 cattgtgtgt agttttgacc aatccattct ttgggtgtgt agccaagtaa actgattgct
     3601 ttgtttacat tttcaaattg gcttgtttgc aaatcaatag taaaaattat ttctttacta
     3661 ctatttaaaa tggttaataa tttttcgttg gattcttgca aagattctac cagcagaaga
     3721 ttttggattt tatgtttttc taaatctgca gctatttggt aagtatcttg aatatctatt
     3781 gtggtaccaa ctactaatgc aggctcacca gtttcatcat acagcagtgt ttgcctcgtc
     3841 tgcacccaaa aatattcatt ggcagttgga ctaaacattc taaatttaat ttcattaaca
     3901 gtattgtttt tttgaagaat aaattgttca taattctgca tcaccctttg catatcatct
     3961 ggatgtgctt tatctaatag ccaatctttt atattgtact gaaatgtttt ttgaggtata
     4021 aaattgccta tacgatgggc aaaccccatt acatgtgtag tatctgtttt taaattatga
     4081 tcccaaatta tttggtttaa tgtatataaa gtagattcat acacatcatt tctaaaaaac
     4141 aattcttctt gaatggttgt tgtgcttgca actttactta ttgctgttga taatttttgt
     4201 aaaatagaag aatatatatt ttcctctaca ggttccttaa aagccaataa tattagttca
     4261 ttgttattat ggttaaagct gtagcctaaa atttgcttaa ttggagaatt ggcaaacaaa
     4321 gaaatatttt cattactatt tgaacgtaag ttaaaagagg aatgagttgg ttgaatttct
     4381 ttaaactgtt tgaaaaattg ttgtacttta ttttcagaga aaattatttg gtcattatta
     4441 gcacaatgat acaatttgtt tgctttatct ctatttacac taatacaaca cattgctgca
     4501 ttagtgtact gaattataag gtttgtaata ttacttaatg catcattaat attgttatta
     4561 ataagttgct cgtaacactg agcaagcaat tgattatttt tggtttctat agattccgta
     4621 acaggcatta gagcatatta tactttcaat tcaaattata tataacgctt tttgtggggt
     4681 ttattattgt attagattta tatattcttt ttaataaagt attgagtaaa aaagtaaggg
     4741 tataattata atactttcag tttaacattt tcaattctgg tatcgctaac acttaaaata
     4801 tcgaactgat aattaccaat aattattgat tctctttgat taggaattga gccataaaaa
     4861 ttaatgatat acccagataa ggtttctgag tcatctacat caaaagttaa tttatatttt
     4921 tctgcaagca tatcaagctc tagccttcca cttaaaacaa attcattttc tgatagttgc
     4981 ttagccacaa aatcatctgt atcatattca tcatgtattt cgccaaaaat ttcttctaaa
     5041 atatcttcca gagttacaat accagcagtt cctccaaact catctaccac ccatgcaata
     5101 tttcttcttt cttttgtaaa cttgctaatt aaatctattg cattcatagc ttctggtata
     5161 acaggaattg gcaataatac atcttgaata tttttaggtt ttttaaataa atctaactga
     5221 tgaatatagc ccaaaatatt atcaatatta tctttaaata caattagcct ggttagtttt
     5281 gtttgtataa aaattttctt tacttcttct atattacttt ctatgtctat cccttctatt
     5341 tcagttcttg gaactaagca ttgccttatt ttaactgtag gtaaactaag tgcattttca
     5401 aataactctt tgtttaattc ttcgccagat tcatcatgtt ctttggcttg ttgaaaaaaa
     5461 tgttctaaat caatatgaga gaaagcttct tctttttcac tcattttaat attaaacaaa
     5521 tattttaaaa cccattgaga aacacttaca aaaaaccttg caatgggtgc aaacaagcta
     5581 tgaaaaatat tagctaaggg tgcaaaaaca ctaatcagca tatcattttt ggctctaaaa
     5641 attgcttttg gaaaaaactc acctaaaact aaaataacta taccagcaat taaagtgttt
     5701 attgcaagtt gaagataccc atttttaaca tgaaagaaac tccaaaaaga gccttgtaaa
     5761 aattggttaa aagcaagccc ataaataacg taaaaaaagt ttaagccaat taagcaagag
     5821 ccaatgaatt cagcgggttt ttctaaaaat tttgctatga cgatcccgct ttttttgccc
     5881 tgttttttct taagttcaac agataattta ttggtgctaa caaaagcaat ttcgtaacca
     5941 gcaaaaaagc caattataat aattgaacaa aaaagccaaa tcaaattgta cacttcttcc
     6001 atagttacga atgtgcacta agttaagcta aaatagtgat aggataaaat acctgttatt
     6061 aatagagtag tataaagaaa aattatctgc tttttaacca atttcttggg tttaaaaaaa
     6121 cagttttatc gttggtaacc ataaaaagta aagattcttc accaaaagtg cttatacccg
     6181 atttccctaa aactgtacct gcctttacct gttggctctt tcttgctgta atttctgaca
     6241 aataattata aattgtaaaa tattttccat gctttacaat tacagcaggc gagcctccaa
     6301 aatctccaac ataggttact tcaccatctg caacagattt tacagcagta ccttgtggaa
     6361 cagctatttc aattccatcg ctatggcctt gtaatttttc tgttatttga taattgccaa
     6421 atggtgcaat aacggtacca atacttacag gccaattcaa attccttttt tcaaaattaa
     6481 ccgacatttc aacaccttct gctgtacttt ctaatgcact atactctcta tttaccgttt
     6541 tggtgcttgc catttcttcc actacctctg tggtggcaac acctggtttt gtagttgtag
     6601 tttttgcaat atttttattg gctgcatttg ctttatttct tgcatctgtt tctgctttta
     6661 actttgccaa ccttgctttt tctgcctctg caatttctct tctaattatt gctgcaatgg
     6721 tgttcctcat ctctgctctt tgcttttctt tttttcttag taaagctgtt acctcttttt
     6781 cttggccttt taatcctttt actacatcat tcttctcttt tttatctgct tctaaatcta
     6841 ctaactgcgc tttttgcgat tctaatactt gattttgctc gtttttagag ttggttaagg
     6901 tgccaatttt ttgttgtaaa acctgttgtg tttttacgat gttgccaact tgtgtttctc
     6961 ttaattttct atagcttttt aagtaagtta ctcttttaag agcatcgtta aaactggtgg
     7021 aagaaaataa aaaatttaaa tattgataat ttcctctatt cttataagca aaagcaatac
     7081 ttcgggcata ttgctgtttt aaggtgtcaa gctctttacg cattctataa atttctaact
     7141 cattttggta aatggtttca tctaaaagcc tcacccttct atttattgtt cttatcaact
     7201 cttctctagc tgcaattttt ctttgtactg atgcaacttg tgctaatgaa acttttttat
     7261 ttttttgaat ttgaactaaa ttcttagata attcattaat ctcttttctt aactcatctt
     7321 ctttcttttg tagttcttct tttgtttgtg catgaagttg aaaagaagca actaatacaa
     7381 aagcaatagc tacaaaaaat tttgcagcat taaataatgc tttaggtgat attttggtta
     7441 atacaataaa acttggtttg tttttcatac aagattttct ttttaagata ccaaaagaat
     7501 agatattaca taaaaagcat aatagctata tagtattgaa aggtcataag taaacaaaaa
     7561 aaatattatt tatgccctat gtgcaaaagt ttactatcaa tgcagtaaaa ctaatggaat
     7621 tgtggagaaa cttttattgc tttttataat ttttaggaat attaaacata tacgttaaag
     7681 gctcgttaaa ggtgtattgt ttaaaatcta accaaacatc taatttagtt ttttctgaaa
     7741 ctgaaaattt tctttttgta gaaaacttta ctccaaaagc atctacataa tcgctgtaag
     7801 taatattaca agttctgttt ttaaaaacat ctatatcatc taacttacta tgcaatactt
     7861 taaaatcgtt tttgtctaaa gtaattaaat gcttaaacaa gttgcccaac ataagtacag
     7921 aaaaatcatc taaactattt ctgtaagaaa ctatattgct atctatataa acaggattac
     7981 caattatgac atcttgaatt gtgctaaaat caaaaggtat ttgtgtaact tcttgcaaat
     8041 aagcaatagt tcttttttga acaattttat caattttatt gattacggta acgctatctt
     8101 ttgtaatttt tgctaccaca ccttctttag taattcccaa aaagctacct actaatctta
     8161 tgtaaattac actatctttt tgcatatacg tataggctgt aagctttctg ctatctgaag
     8221 ggctttcata atctactttt attttagcat aaaaggtaga gaagttgatt ttatgattta
     8281 tagtttgatt caaaatttct ttaacaacag caaaggaatc tattgatttt tcttcggcaa
     8341 cagttacaac ttgagtagta tcttttttag taattgctgt ttgaatatta tgagtatttt
     8401 tggttgtacg gcaagagaat attactacta ataataaagt gcagcctact aataactgtt
     8461 tattcattgt gcaaatttat tgttttcctg tatgtccaaa gcctcctgta cctcttgtag
     8521 tttcgttaat tatttgaacc tgtaccagtt ctgcctgttc aattttacaa aataccattt
     8581 gtgctatacg ttcaccatgt tgtatggtta ctgtttcatt ggaaagattg attaaaataa
     8641 tttttatttc gcctctgtaa tcagcatcta tagtaccagg tgtatttaag caagtaattc
     8701 cttgttttat agccaaacca cttcttggtc taatttgtgc ttcatagcct tgaggtattt
     8761 caataaaaat gccagttggt atcaatgccc tttgtaaagg ttgtaatgta atagcttcgt
     8821 ttatatttgc tcttacatcc ataccagatg ccccttttgt ttcataagta ggtagagggt
     8881 tggttgattt attaataatg ttaatctgca tactattagc caataaatgt tttgacaaaa
     8941 attttaagat ctagtttaaa ggaccagtga aagatataat ttgtatcgca ttgtactctt
     9001 tgttgaatat tttttttatt ctgctctata tcttctacat cgccacgtaa tccatttatt
     9061 tgagaaagcc ctgtaatacc aggtttaact aataaacgct gatgaatatt tggcacatac
     9121 tcgctatata aattatgaaa aggcactgca tgtggccttg gaccaatgat agacatattg
     9181 cctttaagaa catttataaa ctgaggtaat tcatctaaat tatattttct taaaaaaact
     9241 cccactttgg ttgcaggtgt tgtatagcta agtggcaatg aatctttatc tagcgaattg
     9301 ctattgctta aaagcgttct aaacttgtag catttaaaaa ttttaccatt caaaccaata
     9361 cgatcttgca caaacatagc aagccctttg gtttctagtt ttatagcaat tgcaatgatt
     9421 gggtataacc atgaaaaaat aaagaggagg aaacaaatag aaactgcaat atcaaaaaat
     9481 cttttagcca atgatccatt tatttgcaat gccttatatt gcaactgttc attggtaaat
     9541 gaattttgat taaagccgaa agaaaaatta tttttactca ctgttgtttg ttaaacagcc
     9601 gtaaaaatat tggattaata atattatttt cactttagtt caaaatactt ttaagattaa
     9661 actttaaagc ttatggctaa tagttttttt caaactattc cttctgcagt aaaaaaattt
     9721 ttactaagaa gcatagtatt ttttattata tggaaactga tttaccattt ggttttatat
     9781 cctattcgtg ttccagataa acagcttaca catattacag cagttattac taaagaaatg
     9841 atacaatttc attaccctaa tgataaattg aatattgaag aaataaacta tccttcaccg
     9901 aaagaaaatt tattaaaaaa taataaaaaa attgtaggta ttgcagatgg ttgcaatggg
     9961 cttgagttgt atattttata tattggcttt ttagcttgct tcccttctaa taaaaaaaga
    10021 ttatttgctt atgctattac tggcatagta ttaatttttg ttttaaacaa tatacgtagt
    10081 tatgtaatta cattattaaa tattcacaac tcttccttta cagatgttgc ccatcattat
    10141 ttttttaaaa tttttattta tgctgttatg tttttattgt gggtaaaata tacaaaaccc
    10201 aaattaaaag atgaataaaa catttaaaca tattattgct atttctttaa tattaattgt
    10261 ttgttggtgg ggttacacaa atacagaaga aataaataaa atatctaacg tacataagcc
    10321 ttttgagcaa caacttaaaa ggataatttg gttattaatt gtgggaatta ttacttggtg
    10381 ggcttttgcg aaagacaata atcaatggct tagcaaaata gttataatta tttatgcttt
    10441 tgttataatt accattgggc taataggggt acttgaatgg aaatttaagt ttttgagtga
    10501 gaattggaga gaattttttg caggagtaag attattttta tctagtccat tgccttttat
    10561 ttttttatgg ataatgcgta aaattaattt tgatcacttt tctaaaaatt aaaactcttt
    10621 tagaatatta agtaaattag tagagtagga aatattgaaa ataaaatctt tgttatttat
    10681 taaattactt ttctctgttc tataaaatat ttgtgctgca atgcctgaat ttgtaacagg
    10741 attcattaaa taccttatag ccacattaga ataaattaaa ttggttttaa taccttgtgt
    10801 tattttatta ttgaatgaaa aaattcctgt ttgtgataga taatctgatt tataaatatt
    10861 tttaccccaa tgtgaatttg catcatctgc cccataccat gcaataatta agttagcact
    10921 aaataagtat cttttgattt tataatttgc ctctagtaaa aactctctaa aattagcacc
    10981 aaatgggtga gctaaaacct cgccataatg tgtatagctt aaggttggtg ttttactagc
    11041 ataagtataa ggacgaacag tattaaactc aattcttgca aaagcattat ctaaattaaa
    11101 tggcttaaaa gatttaacac caagctgtac accatattta ttttgaaaaa aacctttacc
    11161 attctttgat tctctaatat taaaatcatc tattattaat tgaccataaa ttactgtatg
    11221 tttttttata gtataactag catctacccc tattaaagaa ttatctggag agccgttgtt
    11281 aaagtttaat gagttaaaga aaataattgg aacaaaataa ccccattctt gatctcttct
    11341 atttcctaag ctatctttat tactccaaat aacagattga aataaattta attctacatt
    11401 ttttattccg gtataactaa ctgtattaaa agccccaaat tttttatcgt aaccatttgt
    11461 attaacccaa tttttagaag catctacaaa ctggctccat atagtttgaa aagtaaattt
    11521 gcttatatgt gtttgcattt taaaataagg ataactatac gctacatcgc ttaataataa
    11581 agaacgataa ccattgccaa taaagttttt gttatttcct aattcaaaat aaaaattttt
    11641 gttcggttta tatcctatga aacctattgg aagtgcatac tcaatatttt ttccatttgt
    11701 attgtagcga gctttaccca tgcctggcac aactgttaag gaatctatat attgattaat
    11761 atacttcgga aatttagaaa cattttcgta aatgctgctt gaaataaaaa ctttgttatt
    11821 aaagtttgca tctagttgaa tgcctctatt aaaatggtaa acactatagt cttttgattt
    11881 accaaattca aattgaaata aaggattaat tgttaccgta cttttatcag aaacaaattc
    11941 tgccatattt ccatttctaa atgtttgcgt aaaaaaattt ctttttttcc ttaatgaatc
    12001 tgatggtaat tgataaaaac ttaagagctc tttattaaat ctaatatctg tattgcaata
    12061 tggttgtaat gcagctatac tataaatatt gttttctcta gcccattttt cgtacaactg
    12121 tgcatatagt ttattctgaa ttttaacagg aaacttggct tgaccaattg ctacattaaa
    12181 cttacaaata accaaaattg caagtagaac gtattttaaa aagtatgttt tagtgagcat
    12241 tttcttcacc tcttattata ttataaacag ttaataatac tacttgaata tctaatgcaa
    12301 gcgaccaatt ttctatatac caaatatcat actctactct tttttcaata tcttcaacat
    12361 tagaaatttc gcctctataa ccatgtactt gagcccagcc cgtaatgcct ggttttacaa
    12421 aatgacgaat catgtattta tcaaccatag cagcatattg ctctgtatgc tttaacatat
    12481 gaggtcttgg accaaccaca ctcatgttgc caataaatac atttaaaaac tgcggcaatt
    12541 catcaatact tgtttttcgt aaaaatttgc caattcttgt aattctctga tctccttttg
    12601 tagcttgtat tacatctgca tttacattta ctctcataga cctaaacttg taacaccaaa
    12661 attgtttatt atcttttccg gatcgtagtt gtttaaaaaa tacgggcccc ctcgattcta
    12721 attttattaa taatgctaat aatgggaata gccagcttgc taaaagaatt ataaccaata
    12781 gagaaaatat aaagtcaaaa aaccgcttta gtaatctatt taatatattt tgtaatggct
    12841 cctctcttaa agataacagt ggcagattgc catgaaaact taggttttta aactttgtat
    12901 aaaatctaaa aaaatcggga attactctaa ctcttattgt gttcttatct aatgtttgaa
    12961 tgagattgta caactcttca tctttgatgg aagattttgc aaaaaacact tcatctatat
    13021 tttgtagttg tatagaggcc aaaagatttt caatatactt ttgattattt gcatgaaatt
    13081 cagaatctgg caagtgtcct gcatatacaa agcctaattc ttttttttcg taaaatgttt
    13141 ttttaacttc atctatagca ctttcatctc ctattagcaa tattctttta cctgcataca
    13201 attctaaatt atatcttttt ttaaaataat aaaataccaa cctagccaac caaaagaagg
    13261 tgctaaaaag tataataaaa atgagcaaat cttttttaga aaagaaaaca acagaggata
    13321 aaaagaaatt aatacaaaca acaaacaaaa taaaaatagc taacccatta aaagaatttt
    13381 ttactccttc tttaaaataa attaaatttc ttaaagtata aacattcgta aaaattgttg
    13441 cagcaatcca tgcaaagttg atattaagcc aattaaaaaa atatacttta ttaaacaata
    13501 aatcagatac attaatgaca gatgggctga atcttaaaaa caaaaaaagc agtattatat
    13561 ttaatattgc taaatcgtaa atgaagttta aaaaccttat aaaagctgca tacttatggt
    13621 gcataattga actagccttt ttttaatttt tcttttaaaa ttaatttaat taatgctaag
    13681 ttagtagtaa aatatctcat aaaaagcctg cgtggctctt gtgccattct gtaaaaccat
    13741 tccataccaa ttttttgtac ccaaactggg gcacgtttta ctttgcctgc cactacatca
    13801 aaactaccac caacacccat tacaaaattt aatttttgta atacttgttt gtagttgaat
    13861 aaaaaatttt cttttttagg agaccccatt gcaacaaata aaatatttgc accagaattt
    13921 gctatttcat ttgctatttc gctttcttct tctttattaa aatacccgtt tctatagcca
    13981 gcaattatag aatgcgaaaa ttggtttgta tatttattta ctacctcttt tacaatatgc
    14041 tcttctgccc caaaaaaaaa tattttatac tttttatcat gagcaagttg taccaaattt
    14101 tgcattaaat ctatacctgc cacacgttct ggaatttttt ttcctaaaat ttttgaagcc
    14161 caaacaacac cttgtccatc ggcatttatt atatcgcaat taactacact ttctcttaac
    14221 tcactattat tttgcatgtg tactaattta gccacattaa ccactacatg atgtaaatgc
    14281 tgtttttgaa taattgcatt ttcaatttta ttaagtgttt gatgcatggt tatagcatct
    14341 attgggcagt taaacaattc tattctcatg aaaggctttt ttatatgttg ctaaagcaaa
    14401 aaacatccaa gcttgagccc agcgtatata aggtattttt gaatttaatg attggttaat
    14461 ttgataataa aagtatccat tattatcctg catattttgt agagtccagt ttaatactgt
    14521 actacataat ttttcttctt tttgaattaa ccctaattca tataaagtta ttacaagttg
    14581 tgcaggtgca tgaatatcta taggataggt tttgttatta taatattttg gtttgccatc
    14641 ctcctcgaag aaattattaa tataatattc aaagccttta ctgatagcag tttcaaaact
    14701 tgtatctcct gtgtattttt gataggcagc aatacattct aaattaaacc ctgtatgaaa
    14761 attatctatc caataatgaa aactttgtaa gccataagcc catgcaccat tactttgttg
    14821 atgttttaca cagtaactaa ccgatttttt tgcttcgatt ttataaagct cattttttgt
    14881 taaagaatac attttactaa gcaacctgct gccaagcaaa gaggcattaa aaacaatgct
    14941 tttatctaaa ggagaatagg aaaagcaaaa atctccatca gcatcataat ttctgttaag
    15001 gtcttttaat ataaaactgc aacaactttc tgctgcatct aacaattttt tatcttgtag
    15061 aatttcatac gcttctaaca aagcccatga ggcataaaca gatgcaacaa cagtaggtgt
    15121 atttttaggt tgaaaaaatg cccttgcctg ccaatcaaag ttataacccc aacaataacc
    15181 actccaacca tctgctttca tttcaagcag tttagttgaa agctctttta ttttttgtac
    15241 atttttctct gttggttcaa tggtatataa tctacaatat gcagataaaa aaagtgctat
    15301 tcctttacta ttataccctt tctcaactaa taatagcttt ctaaaattga taggagattt
    15361 tttaaaaccc tgaatccaaa ctaatctgca aaatctatta ttttttataa atggtattgc
    15421 ttgaaatact ttactattca acccatcata agggtcccag cctttataat tttcttcttc
    15481 tacatattgt tgaagtttag aaaaagaatt ttcaaaattt gaattcatta atttaagaga
    15541 gaaattagtt tatgatatcg ccaaacaata accaagtgtt tgtatctgtt tttattaaag
    15601 tagcaacact gtattgattt ttagttttat tggcattaga tgaactgttt agcgtaactc
    15661 cgccatctgc cataaaagta atttgaccaa cacccatttg tgtaactaaa attttagttc
    15721 caataggaaa agcaacactc aattggttag gaattgtaat agtatttata tcaatttgat
    15781 tgatattaat cattttagta gcatctgaaa gtgtaagggt ataatttaaa gtagaaacat
    15841 tattaaactc aactgttctt attaaaaact ttacactatc ctcagttgtt aaaactcttt
    15901 tattgtttga aaaaatatca ttagtaaaat aagctgaagt gtttacattc aactttgttc
    15961 caccaacaaa taattcgcca gtattatttt gcataaagcc ataatttgta atactactgg
    16021 ccatttgtgt atttacaaat tgtgcaaagt ttgtattatt gaagtaagat gttaatctag
    16081 ctttaccatt atacatacct gcttgaatat aatcatctga ttttatatga gaagtaaagt
    16141 aagctttgct acctgcaaaa acaccattag tatttaatgc agtatctagg tttgaataag
    16201 tttgaatttt tattcctcct aaacctatac tatcactact acttgtagaa aaagtgtatt
    16261 taaataaatt atatttaggc gtgccaattc cggtaccaat taccattctg ttattttctg
    16321 caattcttat aaaatcacct ttatggttgg tatgatgatt ataagccaat acaaaatctt
    16381 cattttcgta gtccataccc aaatcccatt tagtgatatc atctaccgaa aacataaaac
    16441 catgtggatt gtgatgcagg cttgaatttt tattcattct taaacccgcc attccactat
    16501 taatattggt attttgtgga ataatttgta aggcaccatt acctaaaaaa tcttgcttaa
    16561 ttaaacctat tgattcaata ttaaaagagt tataaatagg agcataaact actgacacag
    16621 gataattatt atcaggagtt gaagcattaa aagtgtcttg aactagtgtc attgtatttc
    16681 catttagtaa aacaccattc cctttaattc ttctatctct tcttataaaa gagaacccat
    16741 caaaccataa attatgccca ataaaagaat tataagaatt aatattagtt gtatcattac
    16801 ctgcattaat ttgttgaata tttactcctg ctagattttt ggaaataagg cctaaggaat
    16861 tggtagaatt tattttaaat gaatttgcag tagcatttcc acttatttta aaacttgcat
    16921 tttgttcagt agaatcttga atagtaataa acgttgaact atcataacta atgttggagc
    16981 catttatttt cacatatcca tttcctgaaa gttgctcttg tttagcactc caataagcag
    17041 cagaaacaat tacagaatct gcaattgggg tagcagcc
//
LOCUS       DAOQPK010000021        16173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_1820_length_16173_cov_4.661310, whole genome shotgun sequence.
ACCESSION   DAOQPK010000021 DAOQPK010000000
VERSION     DAOQPK010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 16173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16173
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1820_length_16173_cov_4.661310"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1767
                     /locus_tag="PLU36_01960"
     CDS             <1..1767
                     /locus_tag="PLU36_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HMZ45545.1"
                     /translation="FNTSLLTASMFATHTLVAYPIVSKFGVSKNQAVAITVGGTILTD
                     TAVLIILAVIMGNAQGSLNNEFWIKLGVSLSIFSAIMFLLVPRIAKWFFRKLESEKHS
                     HYIFVLSVVFFAAFLAEVAGVEPIIGAFVAGLALNKLIPHSSALMNRIEFIGNSLFIP
                     FFLISVGMLVDVSVLLKGPTAIIVAATLSVVALFSKWFAALFTQLIFKYSKAQRQLIF
                     GLSGAHAAATLAVILVGFKAGILDENILNGTIILILITCIVASFATEKAAKKILIETD
                     EDASNIVKPNGVENEHILLPISNFSNIDKLLEFSGLIKEKKSYHPISMLSVVSDNNEA
                     EANLIKARNKLEEFVKQASASETKVKIITTLDHNDSSGINRISKEIMADIIILQWSER
                     IGFFDKFLGDKTDNIINNSNKSIFICQIAQPLVLQKRIVIVAPPLAEHENGFSIWVTK
                     ISKLAQELSIPIQIYCNKATYKAIEKTQKKFKLNIAFQFIEFEDWEDFLVLSRNILST
                     DLFILISARKSATSYINVLESLPVKLEKYFSNNSRIIIYPQQFVEEIINDQFNDFSSE
                     PLNKSIEAVQRIGKGIGSILKK"
     gene            complement(1774..2103)
                     /locus_tag="PLU36_01965"
     CDS             complement(1774..2103)
                     /locus_tag="PLU36_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HMZ45546.1"
                     /translation="MIHWNDFEKIDIRTGTIISATNFVEAKKPAYKLTIDFGEFGIKK
                     SSAQITNLYELEMLVGKQILAVVNFPPKQIANYISECLVLGVYDSNHQVILLQPDRQV
                     ANGMKVG"
     gene            2217..2690
                     /locus_tag="PLU36_01970"
     CDS             2217..2690
                     /locus_tag="PLU36_01970"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HMZ45547.1"
                     /translation="MSDRLEFMKEAIKLSENGMKNNDGGPFGCVIVKDGKIIGRGWNK
                     VTGTNDPTAHAEVTAIRDACKNLGTFQLDGCEIYTSCEPCPMCMGAIYWARPSKVFYA
                     NTRQDAANIGFDDSMIYHEITLPLTERKIPIEPLGREFALEIFNEWSNKLDKTKY"
     gene            2770..3591
                     /locus_tag="PLU36_01975"
     CDS             2770..3591
                     /locus_tag="PLU36_01975"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HMZ45548.1"
                     /translation="MSNLMQQLNETVNYIKSKVNAQATTAIVLGSGLGNLASVIKTDV
                     AIPYTDIPHFPVSTVKGHGGKLIFGELGGTKVIVMSGRFHFYEGYSAQQVVYPVRVMK
                     MLGVENLLLSNAAGAVNPNYKVGDLMLINDHISFFTTNPLIGRNEEELGTRFPDMSEP
                     YNKAILQKAKSIATANNILVHEGVYTAVTGPTFETHAEYRLIKISGSDVVGMSTVQEN
                     IAAVHCGMKVFAVSVVTDLGIREENNVITHEEVLQAAKEAEPKLTLLFTELVKVL"
     gene            3686..4774
                     /locus_tag="PLU36_01980"
     CDS             3686..4774
                     /locus_tag="PLU36_01980"
                     /inference="COORDINATES: protein motif:HMM:NF024136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HMZ45549.1"
                     /translation="MHNKQVNLLKIFYKEWLTPLIILLCIYLISFFPTVIEKYYSNGI
                     YLYISYILRSISGSVSFSIGDVLYIILVGYLLVQGIKFFAILFSKERRKDFFKYILQT
                     AKKALWLIIFFQILWGMNYYRIGVTHQLNIPKNDYTKEDVESLVCDLVQKLNTIRPAI
                     GRNELPQPSFENIIEESILLHDSLRMKNNLLNYKKASVKKSIFSKYGHYIGFVGYYNP
                     FTAEAQLSVDVPKVLAPYIVCHEIAHQLGYAAEDEANFVGYLSCSQSNNPYFKYSVYL
                     DLYRYAAIELLMMNETNTHKWELDTLVQNDLKDIRKFFLAKANNISPTFSAIYNQYLK
                     ANRQSKGLSSYNNVIGLLISKKKKYGNI"
     gene            4846..4929
                     /locus_tag="PLU36_01985"
     tRNA            4846..4929
                     /locus_tag="PLU36_01985"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4880..4882,aa:Leu,seq:gag)
     gene            5114..5557
                     /gene="rplK"
                     /locus_tag="PLU36_01990"
     CDS             5114..5557
                     /gene="rplK"
                     /locus_tag="PLU36_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HMZ45550.1"
                     /translation="MAKEISGFVKLQVKGGQANPAPPVGPALGSKGVNIMEFCKQFNA
                     RTQDKMGKVVPVLITVYADKSFDFVIKTAPASIQLMEVAKVQKGSKESNRVKVGKVNW
                     QQIEAIALDKMPDLNCFTKESAMKMVAGTARSLGLTVEGNAPWEN"
     gene            5584..6276
                     /gene="rplA"
                     /locus_tag="PLU36_01995"
     CDS             5584..6276
                     /gene="rplA"
                     /locus_tag="PLU36_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HMZ45551.1"
                     /translation="MSLTKKRKVANTKVDKNKVYSLKEASTLVKEINCTKFDSSVDLH
                     IRLGVDPKKADQQVRGTVSLPHGTGKTKRVLVLCTPDKEADAKNAGADFVGLDEFIAK
                     IEGGWTDIDVIVATPSVMPKIGKLGKVLGPRNLMPNPKTGTVTNDVAAAVNEVKGGKI
                     AFKVDKTGIVHASIGRVSFAPDKIAENSTELINAILKLKPATAKGTYLKGVSMASTMS
                     PGITLDTKSLSN"
     gene            6336..6851
                     /gene="rplJ"
                     /locus_tag="PLU36_02000"
     CDS             6336..6851
                     /gene="rplJ"
                     /locus_tag="PLU36_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HMZ45552.1"
                     /translation="MTKEQKNQVIEVLKEKFNQYNNFYITDTESLSVEQVGKLRKACF
                     DKQVEMKVAKNTLIKKALESLDGEKYAGVYESLHNVTALMFSENPKEPAVIISSFRKG
                     SNGEKPTLKAAFINGDVYSGDNQLKALTQIKTKNELIGEVIGLLQSPAKRVLAALLHH
                     HEKQAGAAVAE"
     gene            6951..7325
                     /gene="rplL"
                     /locus_tag="PLU36_02005"
     CDS             6951..7325
                     /gene="rplL"
                     /locus_tag="PLU36_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HMZ45553.1"
                     /translation="MADVKNLAEQLVGLTVKEVQELADVLKSEYGIEPAAAAVVVSAD
                     GGGGAAAVEEKTAFDVILVNGGASKLNVVKIVKELTGLGLKEAKDLVDGAPKPIKEGV
                     SKAEADDLVAKLKEAGAEVEVK"
     gene            complement(7398..8045)
                     /gene="msrA"
                     /locus_tag="PLU36_02010"
     CDS             complement(7398..8045)
                     /gene="msrA"
                     /locus_tag="PLU36_02010"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HMZ45554.1"
                     /translation="MIKSIIFSLFSLSTIASIYFNFKSNNTLKMNDFQIIKEEAYFGA
                     GCFWCTEAIFQQVNGVIKVESGYSGGNLVNPTYEDICNKNTNHAEVVKITFNSKIITY
                     DELLEIFWQTHDPTTLNKQGADIGTQYRSIIFFTNTIQKQKAEDYKNALNKSGAFEKP
                     IVTIIEPIKKFYPAEAYHQNYYINNTSKGYCQFVIKPKIEKFKKVFKQKLKDKIE"
     gene            8150..8542
                     /locus_tag="PLU36_02015"
     CDS             8150..8542
                     /locus_tag="PLU36_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45555.1"
                     /translation="MKKLIVSIIAVLYFTVTSGMVVNFHFCKGKLSSIDFNVSPNKSC
                     ACKVKSKFNKNKSCCKSEYKVVKLNNLHNATYSNIDCKKPFYLAQPAVTFYQIISPKI
                     ATGFLVDNIVKPPISYQKAYIINCVFRI"
     gene            8639..9166
                     /locus_tag="PLU36_02020"
     CDS             8639..9166
                     /locus_tag="PLU36_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HMZ45556.1"
                     /translation="MKSSLLLLSFLVFSFTSFAQDKKEVSEEIKVWGNCGMCKSRIEK
                     TLKGLGVTSASWSSETKMLSVKYNSEKLNNAKIQKAIAAVGHDTQDETASDEVYSKLP
                     GCCKYERKAVATEQKSNVSATTTTAKSCCSKDASKNENAKQECKKDATANKDHKDCAK
                     DGKSESKSCCSKKVD"
     gene            9255..9725
                     /locus_tag="PLU36_02025"
     CDS             9255..9725
                     /locus_tag="PLU36_02025"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HMZ45557.1"
                     /translation="MKKYLIVLFLFLFCLNLNAQPDIGMYSPEIRLTDVNGKSLQLSS
                     LKGKIVLIDFWASWCGPCRAANKKLKKLYDKYKLKGFEIYAISVDYNTNAWKRAIKAD
                     KINWIQVYDENSLIANKWKVSYIPNSYLLNKEGKVVGIDLSPYDLENKIKELLQ"
     gene            9882..12005
                     /gene="metG"
                     /locus_tag="PLU36_02030"
     CDS             9882..12005
                     /gene="metG"
                     /locus_tag="PLU36_02030"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HMZ45558.1"
                     /translation="MNNPTRYLITSALPYANGLKHIGHLAGAYLPADIYTRYLRAQNR
                     EVVFVCGSDEHGTAIPIQAKKEGTTAREIIDKYHPIIEQNFKDLGISFDIYHRTSSPI
                     HHTTAQEFFTELNNNGHLEIKETEQYYDDEAKMFLADRFIKGTCPNCSYNNAFGDQCE
                     KCGKSLSPEELINPVSTLSGKAPIKKLTSHWYLPLNKHEEFLKEWVLNTHKEDWRANV
                     VGNVKSWIDAGLQPRAVTRDLDWGVKVPVKTEENKVLYVWFDAPIGYISATKQWALDN
                     DKDWKPYWYNNDTKLVHFIGKDNIVFHAIIFPVMLKLHGNILPDNVPSNEFMNLEGDK
                     MSTSRGWSIEMEDYINGWIKKENGGESMADVLRYYLTNIAPETKDSEFTWKGFQDANN
                     SELVSIFGNYVNRTFVLMHKLCGGKVPAFHNNLLDETDKQLIIDIAQTKNSVEQLLEQ
                     YKFREAQFEVIDLARKANQYMQKKEPWIVAKTLNETPNNQQLIDNCMHLCLQLTANLA
                     ILINPFLPFTAKKMLYMMKVVDKMLEWKNAGSVKLLSVGYSLREPQLLFRKIEAEEIN
                     EQLEKLKQKAMLGKTEVATSEEVNVEAELKNFKPEIQFDDFTKIDLRIGTIKTAEKVA
                     KADKLLKLEVDLGSEVRTIVSGIAMHFTPEEIINKQVTVVCNLAPRKMRGIESNGMIL
                     MAEDNNGKLHFINPDNVIKEGSSVS"
     gene            complement(12010..13242)
                     /locus_tag="PLU36_02035"
     CDS             complement(12010..13242)
                     /locus_tag="PLU36_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HMZ45559.1"
                     /translation="MNLNVPFFIAKRLAINKKQSFSAFIIKLSIAATALSVAAMIITS
                     AFVNGFQDTVAKKVFSFWGHLRVQYYETSKAFISEENPIPKSMEVENILKKNNQVKHI
                     QPFATKSGVLEKNRNIEGILFKGIDETYDSSTIKPFILQGNWLNFKDSLYSKDIMISS
                     ILANELNIKLYDTVNVFFVSANEGESIYRPVIVKCIYKTGIEEYDKIFVVGDIKLIRR
                     LSNWNEDEIGGYEVILKDYQQMNAVNNNLDLPVIWNSKTIQQIYPNIFEWLQIQDLNK
                     NVMFIIMSIVAIINLITCLLILVLERIKMIGILKAVGYSNSSIQKIFLYHASIIAFIG
                     IIIGCFVGIGLCLLQQYTSFITLDESNYYVSVAPVKIIWGQVFAICGAVLVICFASLS
                     LPVILVKKINPLQAIQFR"
     gene            13405..14685
                     /locus_tag="PLU36_02040"
     CDS             13405..14685
                     /locus_tag="PLU36_02040"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HMZ45560.1"
                     /translation="MKKPFLMLFFLITFYAVLAQEKFSDAIYYPEIKTVQLYQQNNQM
                     SLPIINLGSSDLLELHFDDLDGYVKNYSYTFQLCNENWEPVDLSPFDYIKGFIQNRLN
                     QYRVSSIAQTKYVHYQALLPDRGSVPTKSGNYLLKVFLNGDINKVAFTKKFFVLDNKA
                     SIGLQVLQPYDNSLFRTYQRVQVSVNATQLNPINPQQQVKVVVLQNNRWDNVVRNIKP
                     AFIRGNVLEYNGEQDCLFAAGKEYRWVDLRSFRIATERVHSIDKLTIPNEIILKPDLT
                     RSQLRYAYFKDFNGWYFISSSENINPFWQADYANVHFTFIPPNNKAIEGKDVYIMSNF
                     TNSELNDSTKLLYNEEEGWYEKTFLMKQGYYSYTYVTKDKNNENAIADVTLTDGNLWE
                     TENDYTVFVYYKSVNGRHDELVGITTVNTRLLNR"
     gene            complement(14688..15719)
                     /locus_tag="PLU36_02045"
     CDS             complement(14688..15719)
                     /locus_tag="PLU36_02045"
                     /inference="COORDINATES: protein motif:HMM:NF018378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucuronyl C5-epimerase family protein"
                     /protein_id="HMZ45561.1"
                     /translation="MKNNFSYILLLIVVSIFIASIVSNFKGDDWEENSRKALYFLKGD
                     SVPSTATEYTLSNGVPYVHYVAENGVPAITRFNSTIVANYAIKYAQQFLENSDSTYYK
                     KFLNCVQFLKDSVTQQNNLTFYQFNWQQGWYPNISPPFISSMTNGRAIEVFLYAYQFT
                     NNNYYLQFAKNLLHAFYITVQNNGFAYKLNDSAWWYEEIAKPNVTTPYILDGHIYAIQ
                     GIQKLWLTTKSDSALYLFEKGINALKLKLPSYNAGKGEIYYDAHQHKADKKYHKILTN
                     QIYQLWQSTNDSTFLRYYQQWIKPSKVPYVIRMLKEKNISGLLLIAMLSALFFPILYL
                     VTNKIVLKK"
     gene            complement(15789..>16173)
                     /locus_tag="PLU36_02050"
     CDS             complement(15789..>16173)
                     /locus_tag="PLU36_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HMZ45562.1"
                     /translation="RNNGVLISKFQGATTAYSIDKLQDRGRFFVDPGEEVYIGQVMAE
                     HIKPGDLNVNAIEGKKLTNHRASGSDEGIRITPKILMTLEECMEYIQQDECIEVTPKN
                     IRMRKVILDENERNKAAKSLKVEAV"
BASE COUNT         5915 a         2182 c         2657 g         5419 t
ORIGIN      
        1 tttaatacaa gtttacttac tgcaagtatg tttgcaacac atactttggt agcctatcca
       61 atagttagca agtttggtgt ttcaaaaaac caggcagtgg ctataactgt tggtggtact
      121 attttaacag atactgcagt gctaattatt ttggctgtaa ttatgggtaa tgcacaaggt
      181 agcttaaaca atgaattttg gataaagttg ggtgtttcat taagtatatt ttctgccatt
      241 atgtttttat tagtaccacg tattgcaaaa tggttcttca gaaaattaga aagcgaaaaa
      301 cactctcatt atatttttgt tctttcagtt gttttttttg ctgctttttt ggccgaggtt
      361 gctggtgttg aaccaataat tggtgccttt gtggctgggc tggcattaaa taaattaata
      421 cctcactctt cggccttaat gaacagaatt gagtttattg gtaattcact ttttatacct
      481 ttctttttaa taagtgtagg aatgttggta gatgtaagtg tattattaaa agggccaact
      541 gcaataattg tggcagctac attatcggtg gttgctttat tcagcaaatg gtttgcagca
      601 ttattcacgc aacttatttt taaatattct aaagctcaac ggcaattaat ttttggatta
      661 agtggggcac atgctgctgc tactttagca gtaattttag taggctttaa agcaggtatt
      721 ttagatgaaa acattttaaa tggtactatt attcttattc ttattacttg tattgttgcc
      781 tcatttgcta ctgaaaaagc tgctaaaaaa atattaattg aaacagatga agatgcaagt
      841 aatatagtta aacccaatgg ggtagagaat gaacatattc tattacccat ttcaaacttt
      901 tctaatattg ataaactctt agaattttct ggattaatta aagagaaaaa atcctatcat
      961 cctatatcta tgttatctgt tgtgtcagat aacaatgaag cagaagctaa tttaattaaa
     1021 gcaagaaaca aactggaaga atttgtaaaa caagcatctg catctgaaac aaaagtaaaa
     1081 attattacta ctttagatca taacgattct agcggaatta atagaatttc aaaagaaata
     1141 atggcagata taattatttt acaatggtca gaacgaatag gtttctttga taaattttta
     1201 ggagataaaa cagataatat catcaacaac tctaataaat caatttttat ctgtcaaata
     1261 gctcaaccat tggtactaca aaaaagaatt gtaattgttg cacctccttt agctgaacac
     1321 gaaaacggat tttctatttg ggtaacaaaa atttcaaaac ttgcacaaga gttgagtatt
     1381 ccaattcaaa tatattgcaa taaagctacc tataaagcga tagaaaaaac gcaaaagaaa
     1441 ttcaaattaa atatagcttt tcagtttata gaatttgaag attgggaaga ctttttagtt
     1501 ttgtctcgga atattctctc aacagattta tttatattaa tttctgctcg aaaaagtgca
     1561 acttcttata ttaacgtatt agaaagtttg ccagtaaaat tagaaaaata tttttccaat
     1621 aatagcagaa taattattta ccctcaacaa tttgttgaag aaattattaa cgaccaattc
     1681 aatgattttt cctctgaacc tttaaataaa agtatagaag ctgttcaaag aattggaaaa
     1741 ggaattggtt caatactaaa aaaatagtta ctcttaacca actttcattc cattggcaac
     1801 ttgcctatca ggctgtaata aaataacctg atggttgctg tcgtaaactc ccaaaaccaa
     1861 acactcactt atataatttg cgatttgttt gggaggaaaa tttacaactg ctaaaatttg
     1921 tttgccaacc aacatttcta actcatataa attagttatt tgtgccgatg atttttttat
     1981 tccaaattca ccaaagtcaa tggttaattt ataggcaggc ttttttgctt caacaaaatt
     2041 ggtagcactt ataatggtac cagttcttat atcaattttt tcaaaatcat tccagtgtat
     2101 cattttgttt aatttatttt cttaaaactt aaaactaaat taacaattaa ttttttattc
     2161 attttagtta atcaaattcc tatctttatc aactttgtaa ataaattttt atgcaaatga
     2221 gtgatagatt agagtttatg aaggaagcca ttaaactttc tgaaaatggt atgaagaata
     2281 atgatggtgg tccttttggt tgtgttattg tgaaggatgg aaaaataatt ggacgaggtt
     2341 ggaataaagt aacaggaacc aatgacccaa ctgcacatgc tgaggttact gctattagag
     2401 atgcatgtaa aaacttagga acttttcagt tagatggctg cgaaatttat acttcttgcg
     2461 aaccatgtcc catgtgtatg ggggcaattt attgggcaag accatctaaa gtcttttatg
     2521 ctaatacaag gcaagatgct gctaatattg gattcgacga ctcaatgatt taccatgaaa
     2581 taactttacc acttaccgaa agaaaaattc ctattgagcc attaggcaga gaatttgcct
     2641 tagaaatttt taatgaatgg agtaataaac ttgataaaac aaagtattaa gaaaaattag
     2701 ttaaacatat acacaattct tgtacctaat tagcacaagt atatatttgc agacttaata
     2761 atattttata tgagcaattt aatgcaacaa ttaaatgaaa ctgttaatta cataaaaagt
     2821 aaagtaaatg cacaagcaac tactgcaatt gttttaggaa gcggtttagg taatttagct
     2881 tctgtaataa aaacagatgt agcaattcca tataccgaca ttcctcactt tcctgttagc
     2941 acagtaaaag ggcatggcgg taaacttatt tttggagaac ttggaggcac caaagtaatt
     3001 gttatgagtg gtagatttca tttttacgaa ggctactctg cacaacaagt agtgtaccca
     3061 gttagggtga tgaagatgtt aggcgttgaa aatttattat tatcaaatgc tgctggtgca
     3121 gttaacccaa attataaagt aggagattta atgttaatta acgatcacat tagttttttt
     3181 acaaccaatc cgttaattgg aagaaatgaa gaagagttgg gtactcgttt tccagatatg
     3241 agtgagcctt acaataaggc aattctacaa aaagcaaaat ccattgctac tgcaaataat
     3301 attttagtac acgagggtgt ttacactgca gtaactggcc caacctttga aactcatgca
     3361 gagtatagat taattaaaat tagtggaagc gatgtggttg gcatgagtac tgtacaagaa
     3421 aacatagcag cggtacattg cggaatgaaa gtttttgcag taagtgtagt aacagattta
     3481 ggtattagag aagaaaacaa tgtaattaca cacgaagaag ttttacaagc agcaaaagaa
     3541 gcagagccaa aattaacttt gctatttaca gaattagtaa aggttttata accaattaat
     3601 attttttgaa atccgattat gaaaataatc ggattttttt tataattgaa tcttaattgt
     3661 aaataattat agcaaatttt ttaaagtgca taataagcaa gttaatttat taaaaatatt
     3721 ttataaagaa tggcttacac ctttaattat tttattgtgc atctacctaa ttagtttctt
     3781 ccccactgta attgaaaagt attatagcaa tggaatttat ctatacataa gttatatttt
     3841 acgtagtatt tcaggtagtg taagttttag cattggtgat gttttatata taattttagt
     3901 aggttattta ttagtacagg gtattaaatt ttttgcaata ctattttcaa aagaaagaag
     3961 aaaagatttt tttaaataca ttttacaaac agctaaaaaa gctctttggc taattatttt
     4021 ttttcaaatt ctttggggaa tgaattatta tagaattggc gttacccatc aactaaacat
     4081 accaaaaaat gattacacta aagaagatgt agaaagcctg gtttgtgatt tagttcaaaa
     4141 attaaatact atacgaccag caattggcag aaatgaatta ccacaacctt ctttcgaaaa
     4201 tataatagaa gaaagtattt tactacacga tagcttaaga atgaaaaata atttacttaa
     4261 ctataaaaaa gcttctgtta aaaaaagtat attcagcaaa tatggacatt atatagggtt
     4321 tgtaggatat tataatcctt ttactgccga ggctcaatta agtgtagatg tacccaaagt
     4381 attagcacct tatattgttt gccacgaaat tgctcaccaa ttaggatatg cagcagagga
     4441 tgaagcaaat tttgtaggtt atttatcttg tagccaaagt aacaatccgt attttaagta
     4501 ctcggtttat ttagatttat ataggtatgc agcaattgag ttattaatga tgaatgaaac
     4561 caatacccat aaatgggagc tggatacctt ggttcaaaat gatttaaaag atatacgaaa
     4621 attttttctg gcaaaagcaa ataatatatc tccaaccttt tcagctattt ataaccaata
     4681 tttaaaagcc aataggcaaa gcaaagggtt aagtagttat aataatgtaa tagggttatt
     4741 aattagtaaa aagaaaaagt atggtaatat ttagaataga ttattatttt atttggttag
     4801 atgtaaataa tttattttca cttaatacat ttgcaccttt acattgccca ggtgttgaaa
     4861 ttggtagaca agccactttg aggtggtggt gttcgaaaga acgtgcaggt tcaaatcctg
     4921 tcctgggcac taataatctt aaaatcagct atcttaagtg tatttttaaa attatttcta
     4981 ttatttcaat aaatacactt acttttgcac tcccaaaaag atctttttaa ataagaatga
     5041 ttgaaagcaa tgaataattg cgatatggga gttcatcaaa tgatgaacgt tattaccaat
     5101 taaatctaaa aaaatggcaa aagaaatctc cggatttgtg aagttgcagg taaaaggtgg
     5161 tcaagccaat cctgcacctc cagtaggtcc agcccttggt tctaagggtg taaacattat
     5221 ggagttctgc aaacaattta atgcaagaac tcaagataaa atgggaaaag ttgttcctgt
     5281 tttaattaca gtatatgcag ataagtcttt cgactttgtt attaaaactg caccagcatc
     5341 aattcagtta atggaagtag caaaagttca aaaaggaagt aaagaaagta accgtgtaaa
     5401 agtgggtaaa gtaaattggc aacaaattga agccattgct ttagacaaaa tgccagattt
     5461 gaactgcttt actaaagaaa gtgctatgaa aatggttgca ggtacagcac gtagcttagg
     5521 tttaacagta gaaggtaacg caccttggga aaattaaatt attcaccatt aaaataaatt
     5581 aaaatgtcat tgactaaaaa aagaaaagta gccaatacaa aggtggataa aaacaaagtt
     5641 tattccctta aagaggcttc aacactagta aaagaaatta attgcacaaa atttgatagt
     5701 tcagttgatt tacatattcg tttaggagta gatcctaaaa aagcagatca acaagtacgt
     5761 ggtacagttt ccttaccaca tggtactggt aaaacaaaac gtgtgttggt tttatgtact
     5821 ccagataaag aagcagatgc aaaaaatgcg ggtgctgatt ttgtaggatt agatgagttt
     5881 attgcaaaaa ttgaaggtgg ttggacagat attgatgtaa ttgttgctac cccttctgta
     5941 atgcctaaaa ttggtaaatt aggtaaagta ttaggtcctc gtaacttaat gccaaatcct
     6001 aaaaccggca ctgtaacaaa cgatgttgct gctgctgtaa atgaagttaa aggtggtaaa
     6061 attgcattta aagtagataa aacaggtatt gttcacgctt ctatcggtcg tgtttctttc
     6121 gctcctgata aaatagctga aaatagtacc gaacttatta atgctatatt aaaattaaaa
     6181 ccagctactg ctaaaggtac ttacttaaaa ggagtaagca tggctagcac aatgagccct
     6241 ggtattacat tagacacaaa atcattatct aactaaagcc tgtggattat actttaaaag
     6301 tataaaatga aatgggttta taataaaact tagttatgac taaagaacaa aaaaatcaag
     6361 tgattgaagt tttaaaagaa aaattcaatc aatataataa cttctacatt acagatactg
     6421 aatcgttaag tgtagaacaa gttggtaaat tacgtaaagc ttgttttgat aaacaagttg
     6481 aaatgaaagt agcaaaaaat actttaatta aaaaagcatt agaaagttta gatggcgaaa
     6541 aatatgcagg tgtttatgaa agcctacata atgttactgc tttaatgttt agtgaaaacc
     6601 caaaagaacc tgctgttatc atttcatctt tccgtaaagg aagcaatggc gaaaaaccaa
     6661 cattaaaagc tgcatttatt aatggtgatg tttacagtgg cgataatcaa ttaaaagctc
     6721 taactcaaat taaaactaaa aatgagctta ttggcgaagt aattggttta ttacaatctc
     6781 ctgcaaaacg tgtgttggct gctttattgc accaccacga aaaacaagct ggtgctgctg
     6841 ttgcagaata ataaaaatta aataaccaat gtctctgagg tttaaatgag gcaaatttat
     6901 taaccattcc gctggagata aaacgatgaa agcggttaaa acttaaaaat atggcagacg
     6961 taaaaaactt agctgaacaa ttagtaggct taacagtaaa agaagtacaa gaattagcag
     7021 atgtgttaaa atctgagtat ggtattgaac cagctgctgc agcagttgtt gtttctgcag
     7081 atggtggtgg cggtgctgct gctgttgaag aaaaaacagc atttgatgta attttagtta
     7141 atggtggtgc aagcaaatta aacgttgtta aaattgtaaa agagttaaca ggtttaggtt
     7201 tgaaagaagc taaagattta gtggatggtg ctccaaaacc aattaaagaa ggtgtaagca
     7261 aagctgaagc tgatgactta gtagctaaat taaaagaagc tggtgctgaa gttgaagtaa
     7321 aataattact tcttttctaa tagaattaaa tagtcgtaac tgtaatggtt acgactattt
     7381 tctttaatac actttattta ttctatttta tcttttagtt tttgtttaaa aaccttttta
     7441 aacttttcta ttttgggttt tataacaaat tggcaatatc ctttactagt attattaata
     7501 taataatttt gatgataagc ttcggcagga taaaattttt ttattggttc tataattgta
     7561 acaattggtt tttcaaaagc cccactctta tttaaagcat ttttataatc ttctgctttt
     7621 tgcttttgaa tggtgttggt aaaaaaaatt atacttctat attgtgtgcc aatatctgca
     7681 ccttgtttat ttagagttgt tgggtcatgt gtttgccaaa aaatttcaag taactcgtcg
     7741 taagtaatta tttttgaatt aaatgtaatt tttacaacct ctgcgtggtt ggtattttta
     7801 ttacaaatat cttcataagt tgggtttact aaatttccac cactatatcc actttctact
     7861 ttaattacac cattaacttg ttgaaaaata gcttcggtac accaaaaaca gcctgcacca
     7921 aaataagcct cttctttaat tatctgaaaa tcattcattt ttaatgtgtt attgctttta
     7981 aaattaaaat aaatagaagc tattgttgat aaagagaata aactgaatat tattgattta
     8041 atcatctgat aatcaattta taaggtttaa aaaaatatta ttttttgctt ttgtatattt
     8101 attaaacttt agctatttat ttcatttaaa attaatagtt ttgcaactaa tgaaaaagtt
     8161 aatagtatcc attattgcag ttttatattt tactgttaca agtggaatgg ttgttaattt
     8221 tcatttttgt aaaggaaaac taagttccat agattttaat gtttcaccaa ataagtcttg
     8281 tgcctgtaaa gtaaaatcaa aattcaataa aaataaaagt tgttgtaaat cggaatataa
     8341 ggttgttaaa ttaaataatt tacacaatgc tacctacagt aatattgatt gtaagaagcc
     8401 attttactta gcacagcctg ctgttacctt ctatcaaata atttcaccaa aaatagcaac
     8461 agggttttta gttgataata ttgttaaacc tccaatttct tatcaaaaag cgtatattat
     8521 aaattgtgtt ttcagaattt agaatctgaa aattagccct tagtttttaa tttgtaatat
     8581 cctataaata ttggcaataa aattgctatc tttattaata attttaaata aataaaatat
     8641 gaaatcttcc ttattattat tatcattttt agttttttcg tttacatcat ttgctcaaga
     8701 caaaaaagaa gtttctgaag aaattaaagt ttggggtaat tgtggtatgt gtaaaagcag
     8761 aatagaaaaa acattaaaag gtcttggtgt aacttcagct tcctggagtt ctgaaaccaa
     8821 aatgctatct gttaaataca attctgaaaa attaaataat gcaaaaattc agaaggcaat
     8881 agcagcagtt ggtcatgata cgcaagatga aacagcatct gatgaagttt atagtaaatt
     8941 acctggttgt tgtaaatatg aaagaaaagc tgtagcaact gaacaaaaaa gtaatgtaag
     9001 tgccacaact actactgcaa aatcttgttg cagtaaagac gccagcaaaa atgaaaatgc
     9061 taaacaagaa tgcaaaaaag atgctactgc caataaagat cataaagatt gtgcaaaaga
     9121 tggtaagtca gaatcaaaaa gttgttgctc taaaaaagta gattaatttt tatattattt
     9181 agccttaaat tgaaaaacta agccatcaat ttgttgttta aaaacacttt tgatggcttt
     9241 attattgttg tattatgaaa aaatatttaa ttgttctttt tctttttttg ttttgtttaa
     9301 acttgaatgc acagccagat attggaatgt atagccctga aatacgttta acagatgtta
     9361 atggaaaatc acttcagctt tcatcactaa aagggaaaat agtattgata gacttttggg
     9421 caagctggtg tgggccttgt agggcagcta ataaaaaact aaaaaaactt tacgataaat
     9481 ataagttgaa aggctttgaa atttatgcta tatctgttga ttataatacc aatgcatgga
     9541 aaagggcaat aaaggccgat aaaataaatt ggattcaggt atatgatgaa aatagtttga
     9601 tagccaataa atggaaagta agctatatac caaacagcta tttattgaat aaagaaggta
     9661 aggttgttgg aattgattta tcgccttatg accttgaaaa taaaataaaa gaactcctac
     9721 aataatattg gaaatttatt tgaaagcaat actgctaaat acttaatttg acaagtgaaa
     9781 gcttattttt gtgaacttaa ttttagttgc ataatgtact aacagtacaa ttgtgcgacg
     9841 caacaaaagt ttaatagcat ttaaaagttt gtaacataat aatgaacaat ccaaccagat
     9901 atttaataac aagtgcattg ccttatgcta atggattaaa acatattggg catttagctg
     9961 gtgcctattt acctgcagat atatatacac gttatttacg tgcacaaaac agagaagttg
    10021 tttttgtgtg tggcagcgat gagcatggca ctgccattcc tattcaggca aaaaaagagg
    10081 gcactacggc aagagaaatt atagataaat atcaccctat tattgaacaa aattttaaag
    10141 atcttggaat aagttttgat atttatcata gaacttcatc tcccattcat cataccactg
    10201 cacaagaatt ttttacagaa ttaaataata atggacattt agaaattaaa gaaacggagc
    10261 agtattatga tgatgaagct aaaatgtttt tagcagatag atttattaaa ggcacttgcc
    10321 caaattgctc ttacaacaat gcttttggcg accaatgtga gaagtgtggt aaaagtttaa
    10381 gtccagaaga gttaattaat cctgtatcta ctttaagcgg aaaagcaccc attaaaaaac
    10441 ttacaagcca ctggtattta cctttaaata aacacgaaga atttttgaaa gaatgggttt
    10501 taaacacaca taaagaagat tggagagcca atgtggttgg taatgtaaaa agttggattg
    10561 atgcaggttt acagccaaga gcagttacaa gagatttaga ttggggtgta aaagtgcctg
    10621 taaaaactga agagaataaa gtgttgtacg tttggtttga tgcacctata ggttatataa
    10681 gtgccaccaa acaatgggct ttagataatg ataaggattg gaaaccttat tggtataata
    10741 acgatactaa actggtgcat tttattggaa aagataatat tgtttttcat gcaatcattt
    10801 ttccggttat gttaaaactt catggaaata ttttgccaga taatgtgcca agcaatgagt
    10861 ttatgaattt agaaggagat aaaatgagca caagcagagg atggagtatt gaaatggaag
    10921 attatataaa cggttggata aaaaaagaaa atggtggcga aagtatggca gatgtattgc
    10981 gttattattt aacaaacatt gcaccagaaa cgaaagatag tgagtttaca tggaaaggct
    11041 ttcaagatgc taataatagc gaattagtta gcatttttgg aaattacgta aacagaactt
    11101 ttgtgttaat gcacaaatta tgcggaggta aagtacctgc ttttcataat aacttacttg
    11161 atgaaacaga taaacaactg ataattgata ttgcacaaac aaaaaattct gttgaacaat
    11221 tattagaaca atataaattt agagaagcac aatttgaagt tattgattta gcaagaaaag
    11281 caaatcaata tatgcagaaa aaagaacctt ggattgttgc taaaacttta aatgaaacac
    11341 ccaacaatca gcaattaatt gataattgta tgcatttatg tttacaatta actgctaacc
    11401 ttgctatttt aattaatcca tttttaccat ttactgctaa aaaaatgttg tatatgatga
    11461 aggtggttga taaaatgctg gaatggaaaa atgcaggaag tgtaaaacta ttaagtgtag
    11521 ggtatagttt aagagaacct caattattat tcagaaaaat tgaagccgaa gaaattaatg
    11581 aacaattaga aaaattaaaa caaaaagcaa tgttgggtaa aacggaagtt gctactagcg
    11641 aagaagtaaa tgttgaagct gaattaaaaa attttaaacc agaaatacag tttgatgatt
    11701 ttacaaaaat tgacttaaga attggtacta ttaaaactgc tgaaaaagta gcaaaagctg
    11761 ataaactttt aaaactggaa gttgatttag gaagtgaagt tagaactatt gtttctggca
    11821 ttgccatgca ctttacacca gaagaaatta ttaataagca agtaactgtt gtttgtaatt
    11881 tagcacctag aaaaatgcgt ggtatagaaa gtaatggaat gattttaatg gcagaagata
    11941 ataatggcaa actacatttt attaatccag ataatgttat taaagaaggc agtagtgtaa
    12001 gttaaaacct tatctaaact gtattgcttg taatgggtta atttttttaa ctagtattac
    12061 aggtaatgat agagaagcaa aacaaataac cagtaccgcc ccacaaattg caaatacttg
    12121 cccccaaata atttttacag gagcaacaga tacataataa ttactttcat ctaaggttat
    12181 aaaactagta tattgttgta ataggcaaag gccaatgcca acaaaacagc ctattattat
    12241 tcctatgaaa gcaattatac ttgcatgata tagaaatatt ttttgaatag agctattgct
    12301 atatccaaca gcttttaaaa ttccaatcat ttttattctt tcaagcacta aaatgagtaa
    12361 acaggtaata agattaatga tagccacaat actcattata atgaacatta catttttgtt
    12421 taaatcctga atttgaagcc attcaaaaat attagggtaa atttgttgaa tagttttact
    12481 attccaaata acaggcaaat ctaggttgtt gtttacagca ttcatttgct gataatcttt
    12541 taaaataact tcatatccgc caatttcatc ttcattccag ttgcttaatc tgcgtattaa
    12601 tttaatatcg ccaacaacaa aaattttatc gtattcttca attcctgttt tgtaaataca
    12661 ttttacaatt acaggtctgt aaatagattc tccctcgtta gcagatacaa aaaatacatt
    12721 tacagtgtcg tacagtttta tgtttagttc attggccagg atactagata tcataatatc
    12781 cttactgtat aaactatcct taaaattcag ccaattgcct tgtagtataa atggtttaat
    12841 agtactgcta tcataagttt catcaatacc tttaaataaa ataccttcaa tatttctatt
    12901 tttttccaat acaccacttt tggtagcaaa gggttgaata tgctttactt gattattctt
    12961 ctttaaaata ttctctacct ccatactttt tggtatagga ttttcttcgg aaataaaagc
    13021 tttgcttgtt tcgtaatatt gtactctcaa gtgaccccaa aagctaaata ctttttttgc
    13081 gactgtatct tgaaaaccat taacaaaagc agatgttata atcattgcag ccacacttaa
    13141 agcagtagca gcaatagata gtttaatgat aaaagcagaa aaagattgct ttttattaat
    13201 agctaaacgc tttgctataa aaaaaggtac attcaaattc aaaaatgcat tttgggttta
    13261 caaaaagtaa aacttaaaca aaaattaaaa agtatgtaaa tataatatga ttagtataag
    13321 aattttgttt atggcataat tttaggaact tgaaaaataa taaatgatat ttacgctttc
    13381 aacttttaga agattttatt aattatgaag aaaccatttt taatgttgtt ttttttaata
    13441 actttttatg ctgttcttgc acaagaaaag tttagcgatg caatttatta tcctgaaatt
    13501 aaaactgtac aattatacca gcaaaacaac caaatgagct tacctattat caatttaggc
    13561 tcatcagatt tgttagaatt gcattttgat gatttagatg gatatgtgaa aaactactct
    13621 tatacttttc agctttgcaa tgaaaattgg gagccagtag atttaagccc ttttgattat
    13681 attaaagggt ttattcaaaa ccggttaaat caatatagag tctcatctat tgctcaaacc
    13741 aagtatgttc attatcaagc attgttgcca gatagaggga gtgtgccaac taaaagtggg
    13801 aattatttat taaaagtgtt tttaaatgga gatattaata aggttgcatt tacaaaaaaa
    13861 ttctttgttt tagataataa agcttctatt gggttacaag ttctacaacc ttatgataac
    13921 tctttattta gaacttatca gcgtgtacaa gtaagtgtta atgctacaca actaaaccct
    13981 ataaacccac agcaacaagt aaaagttgta gtgttacaaa ataaccgatg ggataatgtg
    14041 gtaagaaata ttaaaccagc ttttataaga ggaaatgttt tagaatacaa tggagagcaa
    14101 gattgcttat ttgctgcagg aaaagaatac cgatgggtag atttaagaag ttttagaata
    14161 gctacagaac gagtgcatag tattgataaa ttaactatac ccaatgaaat tattttaaaa
    14221 cctgatttaa cacgttctca attaaggtat gcttacttta aagattttaa tggatggtat
    14281 tttatttcaa gttcagaaaa tatcaatcct ttttggcagg cagattatgc caatgtacac
    14341 tttactttta taccaccaaa caataaagct atagaaggca aagatgtgta tataatgagc
    14401 aattttacta atagtgagct aaacgattct acaaaattat tgtataatga agaggaaggg
    14461 tggtatgaaa aaactttttt aatgaagcaa ggttattact cttatacata cgttacaaaa
    14521 gataaaaaca acgaaaatgc cattgcagat gttaccctta ctgatggaaa tttatgggaa
    14581 acagaaaatg attatactgt atttgtatat tataaatcgg taaatggaag gcatgatgag
    14641 ttagttggta taactactgt aaatacaaga ttactaaaca ggtagtatta cttttttaaa
    14701 actattttat ttgttaccaa gtacagaatg ggaaaaaata atgcactaag catagcaatt
    14761 aataacaaac ctgaaatgtt tttttctttc aacattctaa ttacgtaagg tactttagaa
    14821 ggttttatcc attgttgata ataacgtaaa aaggtactat cattagtgct ttgccataat
    14881 tgatatattt ggttggtaag tattttatgg tattttttat ctgctttgtg ttgatgggca
    14941 tcgtaatata tttcaccttt acctgcattg taagatggta gcttaagttt taatgcattt
    15001 ataccttttt caaataagta taaagcagaa tcgcttttag tagttaacca taatttttgg
    15061 ataccttgaa ttgcataaat atgaccatct aaaatataag gtgtagttac atttggtttt
    15121 gctatttcct cataccacca agcagagtca ttaagcttat aagcaaaacc attattttgt
    15181 actgttatat aaaaagcatg gagtaaattt tttgcaaact gcaaatagta attattgttg
    15241 gtaaattgat aagcatataa aaaaacttca atagctctgc cattggtcat gctacttatg
    15301 aagggagggg aaatgtttgg ataccagcct tgttgccaat taaattggta aaaggttaaa
    15361 ttattttgtt gcgtaacaga atcttttaaa aactgaacac aatttaaaaa ttttttatag
    15421 taggtagagt cagaattttc taaaaattgt tgagcatatt ttattgcata attagcaaca
    15481 atggtggaat taaaacgagt aatagcagga actccatttt ctgcaacata atgtacatag
    15541 ggtacaccat tagataatgt gtattctgtt gccgtagaag gtacagagtc tccttttaga
    15601 aagtataaag cttttcttga attttcctcc caatcatctc ctttaaaatt actaacaata
    15661 ctagcaataa aaatagaaac aactataagt agaagtatat atgagaaatt atttttcaat
    15721 atgtaagaat aatatcaaaa ataatatttc tactatacta tataaaaata ttgttataaa
    15781 gtaaaggatt aaacagcttc tactttaagg cttttagcag ccttattacg ttcgttttcg
    15841 tctaaaataa ctttacgcat cctaatgttt ttaggagtta cttcaataca ttcatcttgc
    15901 tgaatatatt ccatacattc ttctaaagtc attaaaattt taggcgtaat acgaatacct
    15961 tcatcagagc cactagctct gtgattggta agctttttac cctcaatagc atttacattt
    16021 aagtctccag gttttatatg ctcagccatt acttgaccaa tatacacttc ttcacctggg
    16081 tcaacaaaaa atctacctct gtcttgtaat ttatctatag agtaagcagt agtagcacct
    16141 tgaaatttag atattaaaac accattattt cta
//
LOCUS       DAOQPK010000022        15863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_1866_length_15863_cov_4.397710, whole genome shotgun sequence.
ACCESSION   DAOQPK010000022 DAOQPK010000000
VERSION     DAOQPK010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 15863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15863
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1866_length_15863_cov_4.397710"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PLU36_02055"
     CDS             <1..454
                     /locus_tag="PLU36_02055"
                     /inference="COORDINATES: protein motif:HMM:NF039585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HMZ45563.1"
                     /translation="FSQQQIAELEKSGSLNIQLSNSEYQLTVSDVEIVAEDIPGWSVA
                     SNSSVTVALDITITEALQSEGDAREFVNRVQNIRKESNFELTDRIFVELEKLNVPESI
                     QYSLNQHKTYICGEILADSIDWKSESLIKGIEIEVNSIMLKIAVTKKA"
     gene            459..1466
                     /locus_tag="PLU36_02060"
     CDS             459..1466
                     /locus_tag="PLU36_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HMZ45564.1"
                     /translation="MATKKPALKKTVKPVKKAAPAKKAAPVKKAALAKKAAPVKKAAP
                     AKKVAPVKKAAPAKKVAPAKKAATKPAAKKVDKSVKKDKKIPKLEKPLKKEVKPEAKK
                     VEKVIKPEKIKKEKAPKPAKEPKVVAPKKILKAGMPLKKTPVKPVKASGIEKSTTVIP
                     MKQKPMIKKEEPVKDVKIPKTSVKTSVAYKPGYTPLENRVEKSAPGAPLVKYSDSDLN
                     EFREIIFKKLEAAKKELAYFQGVITRKDEGGEGEEGRYMTMEDGSLSMEREQLSQMAS
                     RTINYIDNLKKALMRIENKTYGICRVTGKLIDKARLRAVPHATLSLEAKLGLVKPSSA
                     E"
     gene            1640..2416
                     /locus_tag="PLU36_02065"
     CDS             1640..2416
                     /locus_tag="PLU36_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ45565.1"
                     /translation="MITPLKITFLGTGTSSGVPMIGCNCMVCTSRNTKDKRLRSSILV
                     QSATTTIVVDTTPDFRYQMLCTNTKHVDAILFTHPHKDHIAGLDDVRAFNFLTNQPMQ
                     LYANSLTAEALRRDFYYAFSDKKYPGVPNLNIHIIDTNSFFIGDIPIQPILVWHLNMP
                     VFGYKFGNFTYITDANRIDEKEQKLISNSTILVLNALRKEKHISHFTLDEATQLAQVL
                     QAQKTYFTHISHQLGLYNEINNSLPKNIELAYDGLVVEVS"
     gene            2457..3377
                     /locus_tag="PLU36_02070"
     CDS             2457..3377
                     /locus_tag="PLU36_02070"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HMZ45566.1"
                     /translation="MFKEYFTYSKRERNAIIILLLIIIASIIIPFFYQPKFNTNKINI
                     ALADSINALQKRSTTAKKYSKNWSNQQSDFNDEKVTEIILFYFDPNTLDMDGWKKLGL
                     RDKTINTILNYISKGGKFRTPEDIRKIWGLKKEEADRLIPYIRIKPDETYKTSANRTK
                     QVINTNKNEKKSLLDINTVTAWQLKQIPNIGNSLPYKIVTYREKLGGYLFIHQLKEVY
                     EMTDTLYHNILPYIQLIPIKIKKINLNKATEYDLSMHPYIDKNIAKAIVIQRTKYGTY
                     NSIQELKTKIVFLKEEVFLKFEPYITVQDE"
     gene            complement(3386..3844)
                     /locus_tag="PLU36_02075"
     CDS             complement(3386..3844)
                     /locus_tag="PLU36_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ45567.1"
                     /translation="MINIRRAEKADCSRLLELIKELAHFEKAPEEVTVSFEHFVESGF
                     GTNPVWKGFVASTTENNIEKIVGFALYYIRYSTWKGQRMYLEDIIVSEEMRGKGIGGK
                     LFEQLIEEAKNNKLNGIVWQVLNWNEPAIQFYKKYQAKFDDEWINVSINF"
     gene            complement(3856..5310)
                     /locus_tag="PLU36_02080"
     CDS             complement(3856..5310)
                     /locus_tag="PLU36_02080"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HMZ45568.1"
                     /translation="MRKIAVAKGDGIGPEIMDATLKIFNAANVALDYQIVEMGKWVFD
                     KGFSNGMTPLAQQTIEQTGILFKGPMETPKGKGVKSVNVTARKTWNTYANKRTFQTLH
                     GVDSVFSKAGIPIDITIVRENIEDTYGGVEHMLTHDVALSRRFITRPGSMQVIRYAFE
                     MAKKKGAKRITCGHKANIMKITDGLFLDVFYEVAKEYPELNADDVIVDDLCMKLVTKP
                     NLFDVVVLTNLQGDIVSDLCAGLVGGLGFAPSANIGDHISIFEAVHGTAPDIAGKNIA
                     NPTSLLLSGVGMLQHIGLMESASLIENALLYTLESGVHTGDFGDKTTPALNTTEFADT
                     IIANFGKQPKHNPKAYLNDVPFSCSVLHLDKNPMLETTENTGEKIVGVDMFIESNVQP
                     EELAKKCLRHAGVKFKLVNISNRGTQVWPTGSRYTNLVNQYNARFESIDDTALNQQDI
                     IGLYVNLSGDFKICSLELLNSWGDKKSYSLAQGQ"
     gene            5494..6270
                     /locus_tag="PLU36_02085"
     CDS             5494..6270
                     /locus_tag="PLU36_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HMZ45569.1"
                     /translation="MSFFKSGNPTLSQKMFEKSLNAQAEMQGTMSVRGTMNKFGFLLL
                     TVIGGAAYTWQLYVSGQEKLMTTLMIVGVIGGLISALAIAFKPNWAPFLAPAYGILEG
                     FFIGAISVVINNMLAEEGQPNIIMQAVGLTFGVAIAMFVLYNFRIIKPTEKFKSIIVS
                     ATVGIAIFYGITLLLGFFGVKMDFMAFGNGSLLGIGINLFIVAIAALNLILDFDMIEQ
                     GAERGAPKFMEWYGAFGLLVTIVWLYIEILKLLSRFSGRD"
     gene            complement(6350..7225)
                     /gene="sucD"
                     /locus_tag="PLU36_02090"
     CDS             complement(6350..7225)
                     /gene="sucD"
                     /locus_tag="PLU36_02090"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HMZ45570.1"
                     /translation="MAVLVNKNSKIIVQGFTGTEGTFHATQMLEYGTNVVGGVTPNKG
                     GTFHIDKPVFNTVAEAVKTTGANVSIIFVPPAFAADAIMEAADAGIALVVCITEGIPV
                     QDMVKVKNYLQGKSTRLIGPNCPGVITAEEAKVGIMPGFIFKKGKIGIVSKSGTLTYE
                     AADQAVKAGLGISTAIGIGGDPIIGTPTVEAVKLLMDDPETEGIIMIGEIGGSMEADA
                     ARWIKDNKTKPVVGFIAGQTAPPGRRMGHAGAIVGGADDTAAAKMKIMAECGIAVVAS
                     PADIGKTMAAELAKK"
     gene            7427..9589
                     /locus_tag="PLU36_02095"
     CDS             7427..9589
                     /locus_tag="PLU36_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HMZ45571.1"
                     /translation="MKKIHLSIILIVLISFSYAQRGGSLKWNEAGNAYYTTEKGDIVK
                     FSLPKFDKTILVEKKALVPEGKNSELSIKSFSVSTDENVFLIFSNSKKVWRYETRGDY
                     FIYNKNTGKLVQLGKSLSASTLMFAKISPDGKKAAYVSKHNIYVEDLGTNKITKLTKD
                     GTDRIINGTFDWVYEEEFGCRDGFRWNPDSKSIAYWQIDARNIRNFLMINNTDSIYSF
                     TIPVEYPKVGEDPSACKVGVVDIATQKTKWMNVPGSAIQNYITRMEWANNGKLIIQQL
                     DRKQQVSKIYFLNSKTATASLIYTEKDNAWIDIKSRWNNDNPMGWEWVNNNKDFLWVS
                     EKDGWRHIYLISNEGEKETLLTNGEYDMIDFCGIDEKTGFVYFTASPNNALQKYLYKV
                     KLDGTGNAILVSSAEQNGTHSYQVSPNGLYAKHSFSSATIPPSEEWVNLQTGENIKGE
                     IKTKPNKNATTQFFKVTTEDGVTMDGWKILPENFDSTKKYPVVFYVYTEPGSATVRDT
                     YGSASTFLYQGNIAADGYIQISLDGRGTPLPKGAAWRKSIYKKVGILNVRDQAMAAKE
                     ILKWNYVDKSRIAVWGWSGGGSTTLNLLFQYADIYQTGIAIAPVASRLTYDNIYEERY
                     MGLPQENKDDYIQASAITHAKGLKGNLLLVHGTGDDNVHYQNTELLINELVKFNKQFT
                     LMSYPNRSHSISEGKGTFQHLSTLFTNYLKQHCPGGGR"
     gene            9691..9882
                     /locus_tag="PLU36_02100"
     CDS             9691..9882
                     /locus_tag="PLU36_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45572.1"
                     /translation="MPNYNAKEFFYDSLKAKRIENIEYLLNITSEEWKDFEESIFPSN
                     KRRLNDSQKQAIYKPFYTE"
     gene            9898..12675
                     /locus_tag="PLU36_02105"
     CDS             9898..12675
                     /locus_tag="PLU36_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HMZ45573.1"
                     /translation="MKLVLLVFFSVLFHTLLQAQQPHSLLHKADSLATIGEYEAAIKL
                     REQHLQTINSNDTAYNDYIFSLAKLYNANKQYYKAVNLYETSLNGYLKNTKKNKEKLA
                     DNYNNLALAYEAIGRYDKSISYLTKAYNIVRKYYSNNIGSNITGMGNLITALIDNGDM
                     KAAKNLLDTLNEYRANFNHPPLRKEWNKLNAYEKWNRDIFILLTAIRYYANAGDEKNI
                     QTHIAALEKKFTPPLSTEKKNDLAHLLEAYDAIAYQYRISKNYTKALEYYAKAEALVH
                     EDFYKMKAAAHKAIIYNIMGKYQDAYSYVEKALTVFQFPPENNSHYGLIALKAELLQS
                     LHKNKEAVKQLEQLYSLLLKQNITADNFSTFFLNQFTHKINHTQIIIFIKSGNIYYGM
                     FNQSKQKEHLTTATHFYTLAAQLFKTYYSREFYNKALDDLNKQITEKILTVSLEQKNT
                     DYSSAVNLIENNTSKHLWKKFLSRYSENLKTPYELLNKRNMLAAALSNYEILDNKTQE
                     DKKQQQQLNQQIAVLDKEISKTDQFFHTFQSEEFAIKNIQKKLHKNQLIIKYTVADNK
                     VYGLAVTYNNIYLHEIGSIAHLQNQVNLFHAALKNISPIYKKNAETLFTLLVKPFYEE
                     LKNTTDIIIIPEDFLSFLPFEALINPNTNHLLITDYTISYSYALQLWQLQNAANKHIT
                     GTVAFAPDYPNLPLPVKNAITTRGDLYNLENTSKEAAQIVQINKGVLYNGNNATKNNF
                     LNSLGKYGIYHLAMHAQIDNNRYENSNLIFSNDEKLHFYQLYALNFPAQMVVLSACNT
                     GIGTMQHGEGLMSLSRALVYSGVQSSVYSLWEVPDKETAELMILFYKNLGKGQSKDEA
                     LKNAKQQFIKNNPAKSHPYYWAGFVINGNTQPIKNTNYTIWLILVGGVIILLSGWWIK
                     KKYKKTTYSVS"
     gene            complement(12656..13372)
                     /locus_tag="PLU36_02110"
     CDS             complement(12656..13372)
                     /locus_tag="PLU36_02110"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ45574.1"
                     /translation="MDKQTLITQYFENNLSKEALQQFNELLQTDKAFAEEVTFQQQLK
                     AAIHLNKRQELKQQLQQYEQGKVGKINVRKWLYIAAAIIFIAGSGILVLVQKPDAKEL
                     YAAYYQPYPNTVQPIVRGNNDNEKNVFAAFNAYEKGQYETALTLFDELYTIHKKDYPI
                     LYKAICFLQLNQPNESIEILKQYNFTEYEFIVMGKWYLAMAYLKTGDKENAIPLVKYV
                     SENENPFQDAAKTLFKKLNK"
     gene            complement(13365..13889)
                     /locus_tag="PLU36_02115"
     CDS             complement(13365..13889)
                     /locus_tag="PLU36_02115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ45575.1"
                     /translation="MNTIAQLKLGNEATVKDVYNCNKQGFISFAGKYDVSEYEATDIY
                     QDAVIALIENARKGKLDNLKSSLTTYLFAIGKFMLFAYLKKQNKQVMMSIDEAVEWNS
                     YETEQQEEQTALLQKHFEQLGEQCRKMLTLFYYEEKKLEEIMHLMNYDNKDVVKSQKH
                     RCLKQLKNLMGKNG"
     gene            13998..15335
                     /locus_tag="PLU36_02120"
     CDS             13998..15335
                     /locus_tag="PLU36_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ45576.1"
                     /translation="MKALFIVGGIFFMITQLNSQTVWEKTKEKAKEKTEQRRETRTDE
                     AIDKGLDKVEKGIKGIFKKKKKKNESENKETKETANDNETTTPTTSSDNTDTTPVTPI
                     NKGFKAYSKFDFIPGEKITSYEDFSQDAVGDFPAKWSTNASGEVVTFKGVENKWLQFG
                     NNGIFFPEFVNTLPENFTIEFDMAVTDDFSEMQSGLKLYFTPVSNRNLMFDQFFDNKP
                     GVGIDIHPNVTNDENAKSTTKIWVFGKTENKLLENEAAFTGWKISEVNHISVWRQKTR
                     LRIYINETKVWDLPRAFEADIQYAPLFATNIWDGSAFLANLRVAEGLPDTRNKLITEG
                     KFVTNGILFEFQKAEVKPESYAVIKEIATVLKENPTIKIKITGHTSNDGDANANLTLS
                     KQRAAAVQSVLTNEFGIDASRMQTDGKGGSEPVDTTNTPTGKANNRRVEFIKL"
     gene            15375..>15863
                     /locus_tag="PLU36_02125"
     CDS             15375..>15863
                     /locus_tag="PLU36_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45577.1"
                     /translation="MKRIMIAAICFISLQAVKAQIEGITAFEQPSTSKITPKLKVSIN
                     TKQANSIDVSQDNKAFANFTTQQLVDGWERTKTSVYSSSTKDAFGTIAVSIIYLKVKR
                     LPDNSGMWVVRQAGLLNPLTKKTDYSTRDSTLFPNPFFKKTTKPIPGTKLEWHQEFTI
                     SNI"
BASE COUNT         5859 a         2556 c         2470 g         4978 t
ORIGIN      
        1 ttttagccaa caacaaattg cagagttaga gaaaagtgga agcttaaata ttcaactttc
       61 aaactcagaa taccaactta cagtatcaga tgtagaaata gtagcagaag atatacctgg
      121 ttggtcagtt gcatccaatt caagtgtaac tgttgcatta gatataacaa ttacagaagc
      181 cctgcaaagt gaaggagatg caagagagtt tgtaaatcgt gtacaaaata taagaaaaga
      241 aagcaatttt gaacttacag accgtatatt cgtggaatta gagaaattaa atgtgcccga
      301 aagcatacaa tattctctaa atcaacataa aacctatatt tgcggcgaaa tattagctga
      361 ttctattgat tggaagtcag aatctttaat aaaaggtatc gagattgagg ttaatagtat
      421 aatgctaaaa atagcggtaa ctaaaaaagc gtaaatctat ggctactaaa aaacctgctc
      481 taaaaaaaac tgttaaacca gttaaaaagg ctgcaccagc aaaaaaagct gcaccagtta
      541 aaaaggctgc actagcaaaa aaagctgcac ctgttaaaaa ggctgctcca gcaaaaaaag
      601 ttgcaccagt taaaaaggct gctccagcaa aaaaagttgc accagccaaa aaggctgcaa
      661 caaagccggc tgcaaaaaaa gttgacaaat ctgtaaaaaa agataagaag atacctaaat
      721 tagagaaacc tttaaaaaag gaagtaaagc cggaggctaa aaaagtagaa aaagtaataa
      781 agccagaaaa gattaaaaaa gagaaggcac caaaaccagc caaagaacct aaggttgttg
      841 caccaaaaaa gattttaaaa gcaggtatgc ctttaaagaa aacgcctgtt aagccagtga
      901 aagctagtgg aattgaaaaa tctactacag ttattccaat gaagcaaaag ccaatgatta
      961 aaaaagaaga gcctgtaaaa gatgtaaaaa ttcctaaaac tagtgtaaaa acaagtgttg
     1021 cttataaacc tgggtacaca cctttagaaa acagagtgga aaaatctgca ccaggtgcac
     1081 cattagtgaa atatagcgat tctgacttga atgagtttag agaaataata tttaaaaaat
     1141 tagaagccgc taagaaagaa ttggcttatt ttcaaggtgt aattactcgt aaagatgaag
     1201 gtggagaagg agaagaagga agatatatga ctatggaaga tggtagtttg agtatggaaa
     1261 gagagcaact tagccaaatg gcaagcagaa ccataaacta tatagataac ttgaagaaag
     1321 ccttaatgcg aattgaaaat aaaacttatg gcatttgccg tgttactggt aaattaattg
     1381 ataaagcccg tttacgtgct gtgccacatg caactttaag tttagaggct aaacttggtt
     1441 tggtaaaacc atcatctgct gaataacaat attagtaata aatatagctt taagccccga
     1501 aaggggcttt ttttatttac agtaaattgg tgaactaaga aatgaataaa aaattaattt
     1561 ataaatattg aaggaatctt taactattaa ataatttata gaataatatt atataaattt
     1621 taatgtaaat ttgaatgtaa tgattacgcc tttaaaaatt acttttttag gaacaggtac
     1681 aagtagcggt gtaccaatga ttggctgcaa ttgtatggtt tgtacctcaa gaaatactaa
     1741 agataaaagg ctaagaagta gcattttggt tcaatctgct acaacaacta ttgtggtaga
     1801 tactacacca gattttagat atcaaatgtt gtgtactaac actaaacatg tggatgccat
     1861 tttatttact cacccccata aagatcatat tgcaggatta gatgatgtaa gagcatttaa
     1921 tttcttaact aatcagccta tgcaattata tgccaactct ctaactgccg aagctttgag
     1981 aagagatttt tattatgcat tttcagataa aaaatatcct ggcgtgccaa atttgaatat
     2041 ccatattatt gacacaaata gcttttttat tggcgatatt ccaatacaac ctattttggt
     2101 ttggcattta aatatgcctg tatttgggta caaatttgga aactttactt atataactga
     2161 tgcaaataga attgatgaaa aagaacaaaa attaattagt aattcaacaa ttttggtttt
     2221 aaatgcatta agaaaagaaa agcatatttc tcattttacg ttagacgaag ctacccaatt
     2281 agcccaagta ctgcaggccc aaaaaactta ctttacacat atcagccatc aacttggttt
     2341 atataatgaa ataaataaca gcttacccaa aaatattgaa ttagcttatg atggattagt
     2401 tgtagaagta agttaagaaa agttagtact tacagataaa taattgaaat gtaaagatgt
     2461 ttaaagagta ttttacatat tcaaaaagag aaagaaatgc aataataatt cttctactta
     2521 ttattattgc tagcatcatt attccctttt tctaccaacc aaaatttaat accaataaaa
     2581 taaatattgc tttggcagat tctattaatg ccttacaaaa aagaagtact actgcaaaaa
     2641 aatattctaa aaattggagt aatcaacaat cagattttaa tgatgaaaag gttactgaaa
     2701 ttattttatt ttattttgac cccaatacct tagatatgga tggttggaaa aaattaggac
     2761 taagagataa aacaataaac actattttaa actatatatc taaaggagga aaatttagaa
     2821 caccagaaga cattagaaaa atatggggct taaaaaaaga agaggcagat agattaatac
     2881 cctatataag aataaagcct gacgaaactt ataaaacaag tgccaacaga acaaagcaag
     2941 ttattaatac aaacaaaaat gaaaaaaaat ctttactaga tattaatact gtaacagctt
     3001 ggcagcttaa acaaataccc aatataggca actcattacc ttataagata gttacttata
     3061 gagaaaagtt gggaggttat ttatttattc atcagctaaa agaagtgtat gaaatgacgg
     3121 atacattata ccacaatatt ttaccttata tacaattgat accaataaaa attaaaaaaa
     3181 ttaatctaaa caaagcaaca gaatatgatt taagtatgca cccctatatt gataaaaata
     3241 ttgctaaagc tatagtaata caaagaacga aatatgggac ttataattcc atacaagaat
     3301 taaaaaccaa aatcgttttt ttaaaagaag aagttttttt aaagtttgaa ccttatataa
     3361 ctgtacaaga tgaatagagt atagtctaaa aatttatact aacatttatc cattcatcat
     3421 caaattttgc ttggtatttt ttataaaatt gaatagctgg ttcattccag ttcagcactt
     3481 gccaaacaat accatttaat ttgttatttt ttgcctcttc aattaattgt tcaaataatt
     3541 taccaccaat tccttttcct ctcatctcct ccgaaacaat aatatcttct aaatacattc
     3601 tttgcccctt ccatgtacta tatctaatat agtataatgc aaagcctaca attttttcta
     3661 tattattttc tgttgtagaa gctacaaagc ccttccatac agggtttgtg ccaaaaccac
     3721 tttctacaaa atgctcaaaa gatactgtta cttcttcggg agctttttca aaatgtgcaa
     3781 gttcctttat caattctaat aaacggctac agtctgcttt ttcggctctt ctaatgttta
     3841 tcatccttta tattattact gaccttgtgc aaggctataa ctttttttat cgccccagct
     3901 atttaataat tctaaactac aaattttaaa atcgccactt aggttaacat ataaaccaat
     3961 tatatcttgc tggtttaagg cagtatcgtc tatactttca aaacgagcat tgtattgatt
     4021 tactaaattg gtataacggc taccagttgg ccaaacttgt gtgcctctat tactaatatt
     4081 aactaactta aattttacac ctgcatgcct taaacatttt tttgctaatt cttctggttg
     4141 cacattgctt tcaataaaca tatctactcc aacaattttt tcaccagtgt tttctgttgt
     4201 ttcaagcatt gggtttttat ctaaatgtaa aacagagcag gaaaagggta catcatttaa
     4261 ataagcttta ggattatgtt ttggttgttt accaaaattg gcaataatag tatctgcaaa
     4321 ctctgtagta tttaatgcag gggttgtttt atctccaaaa tctcctgtat gtacaccact
     4381 ctctaaagta tataataaag cattttcaat taaacttgca ctttccatta atcctatatg
     4441 ctgcaacatg ccaacaccac ttaacaataa agaagttgga ttagcaatat tttttccagc
     4501 aatatctggt gcagtaccat gtacagcttc aaaaatagaa atatggtctc caatattggc
     4561 acttggtgca aagcctaacc caccaactaa gcctgcacat aaatcgctta caatatctcc
     4621 ttgtaaatta gttaaaacaa ccacatcaaa taaattgggt ttagttacta acttcataca
     4681 taaatcatca acaattacat catctgcatt taattcggga tattctttag caacttcata
     4741 aaacacatct aaaaataagc catcggtaat tttcataatg tttgctttat gcccacaggt
     4801 aattcttttg gctccttttt tctttgccat ttcaaaagca tatctgataa cttgcatgct
     4861 acctggtctg gtaataaatc ttctgcttaa agcaacatca tgagtaagca tgtgttcaac
     4921 accaccataa gtatcttcaa tattttctct tacaatagta atatcaatag gaataccagc
     4981 tttgctaaaa acagaatcaa ccccatgtaa tgtttgaaaa gttcttttat ttgcataagt
     5041 gttccaggtt ttacgtgctg taacatttac actttttaca ccttttcctt ttggcgtttc
     5101 cattggtcct ttaaataaaa tacctgtttg ctcaatagtt tgttgtgcta aaggtgtcat
     5161 gccattgcta aaacctttat caaaaaccca tttacccatt tcaactattt gatagtctaa
     5221 agcaacatta gctgcattaa atatttttaa agtagcatcc ataatttctg ggccaatgcc
     5281 atctcctttt gcaactgcta ttttcctcat tgtactttat ttttgaggtg caaaagtaag
     5341 ggttgaaagt gtagtaactt gtgaaagaaa aagtaaataa ataattttat tatggatatt
     5401 aaataggtaa tttttataca ttgttaaatt gtaagagaat tgatagattt taatctgcaa
     5461 ttttttatct ttacagttca aaattaaaaa actatgtctt ttttcaaatc cggaaaccca
     5521 actttatctc aaaaaatgtt cgagaaaagc cttaatgctc aggcagaaat gcaaggtaca
     5581 atgagcgtta ggggaacaat gaataagttt ggctttttat tacttacagt tattggtggt
     5641 gcagcttaca cttggcaatt atatgttagt ggtcaggaaa aattgatgac tacacttatg
     5701 attgttggtg taattggagg cttaatttca gctcttgcaa ttgcttttaa acctaattgg
     5761 gctccttttt tagctcctgc ttacggtatt ttagaagggt tttttatagg tgcaatctct
     5821 gttgttatta ataatatgct tgcagaagaa gggcaaccta atattattat gcaagctgtt
     5881 ggtttaactt ttggtgttgc aattgcaatg tttgtattgt ataatttcag aattattaaa
     5941 cctaccgaaa aatttaaaag tattattgtt agtgctacag taggtattgc aattttttat
     6001 ggcataacct tattgttggg cttttttggc gtaaaaatgg attttatggc ctttggtaat
     6061 ggtagtttat taggtattgg tataaaccta tttatagttg ctattgctgc cttaaattta
     6121 attttagatt ttgatatgat agaacaagga gccgaaagag gtgcccctaa atttatggag
     6181 tggtatggtg cttttggttt attggtaact attgtttggt tatatattga aattttaaaa
     6241 ctattaagcc gtttctctgg cagagattag aagagaaata caggtaaaaa tttaaaagcc
     6301 ccttgtacat tacaacaagg ggcttttaaa tttatttaat gcttattttt tattttttag
     6361 ctaattcggc agccattgtt ttaccaatat ctgcagggct tgcaacaaca gcaattccac
     6421 attctgccat aattttcatt ttagcagcag cagtatcatc tgcaccacca acaattgcac
     6481 cagcatgacc cattctacga cctggaggtg ctgtttgtcc agcaataaaa cctactactg
     6541 gttttgtttt attgtcttta atccaacgtg cagcatcagc ttccatactt cctccaattt
     6601 cgccaatcat aataatacct tctgtttcag ggtcatccat taaaagtttt acagcttcaa
     6661 cagttggtgt gccaattatt gggtctccac caataccaat agcagtagat atacctaaac
     6721 ctgcttttac agcttgatcg gcagcttcgt aagttaatgt accagattta gaaacaatgc
     6781 cgattttccc tttcttaaat ataaagccag gcataatacc aactttggct tcttctgcag
     6841 taattacacc agggcaatta ggaccaatta accttgtact tttcccttgt aaataatttt
     6901 tcacttttac catatcttgt acaggaatac cctcggtaat gcaaacaact aaagcaatac
     6961 ctgcatcggc agcttccata attgcatcag cagcaaaagc tggtggtaca aatatgatac
     7021 taacattggc tcctgtggtt tttactgctt ctgcaacagt attaaatact ggtttatcta
     7081 tatggaaagt tccgccttta ttgggtgtta caccaccaac aacattggtt ccatattcta
     7141 acatttgagt tgcatgaaaa gtaccttcgg tacctgtaaa accctgaaca ataattttag
     7201 agtttttatt aactaatact gccatttgaa gattttttgt gtcgcaaaaa taagagaaac
     7261 taaagcattg gcaatataaa atttaaaaga ctacccttct tttttagttg ttttaaattg
     7321 tttattaata gaagtaaatg taaagttgaa ctaccactta taattttaaa gtatgtttta
     7381 ttattattgt actattaaaa ctatattttg caaaaaaatt aaagaaatga aaaaaataca
     7441 tcttagcatc atactaattg ttcttatttc tttttcttat gcacaacgtg gtggtagttt
     7501 aaaatggaat gaggctggaa atgcttatta tactaccgaa aaaggagata ttgtaaaatt
     7561 tagcttacct aaatttgata aaaccatttt agtggagaaa aaagcattag tacctgaagg
     7621 gaaaaattcg gaattaagta ttaaaagctt ttcagtaagt actgatgaaa atgttttttt
     7681 aatttttagt aatagtaaaa aagtttggag atatgaaaca agaggggatt attttattta
     7741 taataaaaac acaggaaaat tagttcaatt gggtaaatca ctaagtgcat ctacattaat
     7801 gtttgctaaa atttctcccg atggaaaaaa agctgcgtat gtaagcaaac acaatattta
     7861 tgttgaggat ttaggtacta ataaaataac taagctaaca aaagatggta cagatagaat
     7921 aattaatggc acttttgatt gggtatatga agaagagttt ggctgtagag atggatttag
     7981 atggaaccca gatagtaaaa gcattgcata ttggcaaata gatgcaagaa atattcgcaa
     8041 ctttttaatg ataaataata ccgactctat ttattctttt accattcctg tagaatatcc
     8101 taaagttggc gaagacccat cagcttgtaa agtaggtgtg gtagatattg caacccaaaa
     8161 aacaaaatgg atgaatgtgc caggaagtgc tattcaaaat tatattacaa gaatggagtg
     8221 ggccaataat ggtaaactta taattcagca acttgataga aaacaacaag taagtaaaat
     8281 ttatttttta aactcaaaaa ctgcaactgc atctttaatt tatactgaaa aagataatgc
     8341 atggattgat attaaaagta ggtggaataa cgataatcca atgggttggg aatgggtgaa
     8401 taataataaa gactttttat gggttagcga aaaagatggt tggaggcata tttatttaat
     8461 aagcaacgaa ggggaaaaag aaaccttatt aactaatgga gagtatgata tgattgactt
     8521 ttgtggtata gatgaaaaaa caggctttgt atattttacg gcatcgccaa acaatgcatt
     8581 gcaaaaatat ttgtataaag ttaagttaga tggaacaggt aacgcaattt tagtttcctc
     8641 tgctgaacaa aatggcactc atagttatca ggtttctcca aatgggttat atgcaaaaca
     8701 tagtttttct agtgccacta taccaccatc tgaagagtgg gttaatttgc aaacaggaga
     8761 aaatataaaa ggagaaatta aaacaaagcc aaataaaaat gcaactaccc aattttttaa
     8821 agtaaccaca gaagatggcg taactatgga tggctggaaa attttaccag aaaatttcga
     8881 ttctaccaaa aaataccctg ttgtgtttta tgtatatacc gagccaggct ctgctactgt
     8941 tagagatact tatggtagtg caagtacttt tttgtatcaa ggaaatattg ctgctgatgg
     9001 ctatattcag attagccttg atggaagagg aaccccattg cctaaaggtg ctgcttggag
     9061 aaaatctatt tataaaaaag ttggaatttt gaatgtaaga gaccaagcaa tggctgcaaa
     9121 agaaatttta aaatggaatt atgtagataa aagcagaatt gcagtttggg gctggagtgg
     9181 tggtggctca actactttaa acttattatt tcaatatgcc gatatctatc aaacaggcat
     9241 tgctattgca ccagttgcaa gcaggttaac ttatgataat atatatgaag aacgctatat
     9301 ggggttgcca caagaaaata aagacgacta tattcaggct tctgccataa cacatgcaaa
     9361 aggattgaaa ggaaatttac tattagtaca tggaacagga gatgataatg tgcattatca
     9421 aaacacagaa ttgttaatta atgaattggt taaatttaat aagcagttca ctctaatgtc
     9481 ttaccctaat cgttctcata gtattagcga aggcaaaggt acttttcaac atttatcaac
     9541 actatttaca aattatttaa aacaacattg cccaggaggt ggaagatagt tcttgcacta
     9601 acgatatttt tagttttttg ctttaattga ttttgagtgc taatgatgta attttaaaaa
     9661 aaatattttt tttaagtagg taaccaacct ttgccaaact acaatgcaaa ggagtttttc
     9721 tacgattcgt taaaagctaa aagaattgaa aatattgagt atttattaaa cataacaagt
     9781 gaagagtgga aagactttga agaaagcatt tttccatcaa ataaaagaag gctaaatgat
     9841 tctcaaaagc aagccattta caaaccattt tatacagaat aaacctgttt atacaagatg
     9901 aaattagtat tgcttgtctt tttttccgtt ttgtttcata cactgctgca agcacagcaa
     9961 ccccattcct tactacacaa agcagacagt ttagcaacaa taggggaata tgaagctgct
    10021 ataaaactaa gagagcaaca cttacaaaca atcaattcca atgacactgc atacaacgat
    10081 tatatatttt ccttagccaa attatacaat gcaaataaac aatattataa ggctgtaaat
    10141 ctttatgaaa cttctttaaa tgggtattta aaaaacacta aaaaaaataa agaaaaatta
    10201 gctgataact acaacaacct tgctcttgct tatgaagcta ttggcaggta cgacaaaagc
    10261 atcagctatc ttaccaaagc atacaatatt gtaagaaaat attacagcaa caacataggc
    10321 agtaacataa caggtatggg aaaccttatt actgctttaa tagacaatgg cgatatgaaa
    10381 gctgccaaaa atctgttaga tacattgaat gaatacagag caaatttcaa tcaccctcct
    10441 ttacgaaaag aatggaacaa actgaacgct tatgaaaaat ggaatagaga tatttttatt
    10501 ttacttacag ccataagata ttatgccaat gcaggtgatg aaaaaaatat acaaacacat
    10561 atagcagcat tagaaaaaaa atttacaccg cctttatcca cagaaaagaa aaatgattta
    10621 gcccatttat tggaagccta tgatgcaata gcatatcaat atcgcataag caagaattat
    10681 acaaaagcat tagaatatta tgccaaagcc gaagctcttg tgcatgaaga tttttacaaa
    10741 atgaaagccg ccgcccacaa agctattatt tataatataa tgggcaaata tcaagatgct
    10801 tatagttatg tagaaaaagc attaactgtt tttcagtttc ccccagaaaa caacagtcat
    10861 tatggtttaa tagctttaaa agcagagcta ttacaatcgt tgcataaaaa caaagaagct
    10921 gtaaagcaat tagaacaact ttacagccta ctccttaaac aaaatattac tgcagataat
    10981 ttttcaactt ttttcctcaa ccagtttaca cataaaataa accatacaca aattattatt
    11041 ttcataaagt ctggaaatat ttattatgga atgttcaacc aaagcaaaca aaaagaacat
    11101 cttaccacag caacacattt ttatacatta gctgcacaat tatttaaaac ttattactcc
    11161 agagaatttt acaataaagc attagatgac ctgaacaaac aaataacaga aaaaatattg
    11221 actgtatctc ttgaacaaaa aaatacagat tactcatcag ccgtaaatct tatagaaaac
    11281 aacacatcca aacatttatg gaaaaaattt ctgagccgtt acagcgaaaa tcttaaaacc
    11341 ccttacgaat tgctgaacaa aagaaatatg cttgcagcag cattaagcaa ttacgaaata
    11401 cttgacaata aaacccaaga agataaaaaa caacagcaac aactaaacca acaaattgca
    11461 gtattagaca aagaaattag caaaacagac caattttttc acacattcca atcggaagag
    11521 tttgccatca aaaacattca aaaaaagctg cacaaaaacc aactcattat aaaatatact
    11581 gtggcagata ataaagtata tggtttagct gttacttaca ataatattta tttgcacgaa
    11641 ataggcagta ttgctcactt acaaaatcag gtcaatttgt ttcatgcagc tttaaaaaac
    11701 atcagcccta tatataaaaa aaatgctgaa acattattta cattattagt aaagcccttt
    11761 tacgaagaat taaagaacac aactgacata attataattc cagaagattt tttaagtttt
    11821 cttccctttg aagcattgat taatcctaat accaatcatt tattaattac agactacact
    11881 atcagttatt cttatgcttt acaattatgg caactccaaa atgcagccaa caaacacatt
    11941 acaggcacag tagcttttgc accagattat ccaaatttac ctttacccgt taaaaatgct
    12001 attaccacac gtggcgattt atacaattta gaaaatacca gcaaagaagc agcacaaata
    12061 gtacaaatta acaaaggtgt attgtacaat ggcaacaatg ctacaaaaaa caactttcta
    12121 aacagtcttg gcaaatatgg catttatcat ttagccatgc acgcccaaat agacaacaat
    12181 cgttacgaaa actccaacct tattttcagt aatgatgaaa agctacattt ttatcaacta
    12241 tatgcattga attttcctgc tcaaatggta gttttaagtg cctgcaatac aggtattggc
    12301 actatgcaac atggcgaagg attaatgagt ttatcaagag cattagtata tagtggtgtg
    12361 caatcttctg tttattcttt atgggaagtg cctgataaag aaacagcaga actaatgatt
    12421 ttattttata aaaatcttgg taaaggtcaa tcaaaagacg aagcattaaa aaatgccaaa
    12481 caacaattta ttaaaaacaa tcctgcaaaa tcgcatccct attattgggc aggatttgta
    12541 ataaacggca atacacaacc cataaaaaat actaactaca caatatggct tatacttgtt
    12601 ggtggagtaa taattttatt aagcggttgg tggataaaga aaaaatataa gaaaactact
    12661 tattcagttt cttaaataaa gtctttgctg catcttgaaa agggttttca ttttccgata
    12721 catattttac caaaggaata gcgttttctt tatcacctgt ttttaaataa gccattgcca
    12781 aataccattt acccattact ataaattcat attcggtaaa attgtattgc tttaaaattt
    12841 ctatagattc atttggctga ttaagttgca aaaaacaaat ggctttataa agaataggat
    12901 agtctttttt gtgtattgtg tataattcat caaataaagt taaagccgtt tcatattgtc
    12961 ctttttcata agcattaaaa gcagcaaaca catttttttc attatcatta ttgcctctta
    13021 caattggttg cacagtattt ggatagggtt gataatatgc agcatacaac tcttttgcat
    13081 caggcttttg cacaagaacc aatattccag aacctgcaat aaatataatg gcagcagcaa
    13141 tgtacagcca ttttcttaca ttgattttcc ctacttttcc ttgctcatat tgttgtaatt
    13201 gttgtttcag ttcctgcctt ttgtttaaat gtatagctgc ttttagctgt tgttgaaaag
    13261 ttacttcttc tgcaaatgct ttatccgttt gcagcaattc attaaactgc tgcaatgctt
    13321 ccttgctcaa attattttca aaatattgtg ttatcaatgt ttgcttatcc attttttccc
    13381 atcaggtttt ttaattgctt aaggcatcta tgtttttggc tttttactac atctttatta
    13441 tcataattca tcaggtgcat tatttcttcc aatttttttt cttcataata gaacaacgtg
    13501 agcatttttc tacattgttc tcctaattgt tcaaaatgtt tttgcagcaa agccgtttgt
    13561 tcctcttgct gttctgtttc ataactgttc cattcaacag cttcatctat actcatcatt
    13621 acttgtttgt tttgcttttt cagataagca aacaacataa attttcctat ggcaaataaa
    13681 tatgtagtta aagaactttt taaattatcc aattttcctt ttcgagcatt ttcaattaat
    13741 gcaattactg catcttgata tatatctgtt gcttcatatt cagaaacatc atacttacct
    13801 gcaaaagaaa taaatccttg cttattacaa ttataaacat cctttactgt tgcttcattc
    13861 cccagtttta gctgtgctat tgtattcatt agtgtaaata atgtagtaaa agtaaacgca
    13921 aagaaaaaaa taaaaaataa tgtttacttt tttctgtttt agcacactaa gtatttatac
    13981 agctaaaaaa actaaatatg aaagcattat ttattgtagg gggcattttt tttatgatta
    14041 cacagttaaa ctcccaaacc gtatgggaaa aaaccaaaga aaaagccaaa gaaaaaacgg
    14101 aacaaaggag agaaaccaga accgatgaag ctattgacaa aggattagat aaagtagaaa
    14161 aaggcataaa aggaattttt aaaaagaaaa agaagaaaaa cgaatcagaa aataaagaaa
    14221 caaaagaaac agcgaatgat aatgaaacta ctacacctac cacttcttca gataatacag
    14281 atactactcc tgtaacgcct ataaacaaag gctttaaagc ttacagcaaa tttgatttta
    14341 tacccggtga aaaaataaca tcttatgaag atttttcgca agatgccgtt ggcgattttc
    14401 ccgcaaaatg gagtaccaat gcatcaggcg aagtagtaac ttttaaaggt gtagaaaaca
    14461 agtggctaca atttggcaac aatggtattt ttttccccga atttgtaaat acactacccg
    14521 aaaattttac catagaattt gatatggctg taacagatga tttcagtgaa atgcaatctg
    14581 gactgaaatt gtattttaca cctgtaagca accgcaactt aatgtttgac cagttttttg
    14641 acaacaaacc aggtgtaggc atagacattc atccaaatgt aaccaatgac gaaaatgcaa
    14701 aaagcactac taaaatttgg gtttttggta aaacagaaaa taaactgtta gaaaatgaag
    14761 ctgcttttac aggttggaaa atttcagaag taaaccatat ttctgtttgg cgacaaaaaa
    14821 caagactaag aatatatatt aatgaaacca aagtgtggga tttgccaaga gcatttgaag
    14881 cagatataca gtatgctcct ttatttgcca ccaatatatg ggatggcagt gcttttcttg
    14941 ctaatttacg tgttgcagaa ggcttgcctg atacaagaaa caaactgatt acagaaggaa
    15001 aatttgttac taatggtatt ttgtttgaat ttcaaaaagc agaagtaaaa cctgaaagct
    15061 atgctgtaat taaagaaata gcaactgtat taaaagaaaa tccaaccatt aaaataaaaa
    15121 taacaggaca caccagcaat gatggcgatg ccaatgccaa tcttacttta tctaaacaaa
    15181 gagctgctgc tgtgcaaagt gttttaacca atgaatttgg catagatgct tctcgtatgc
    15241 aaacagatgg caaaggaggc agcgagcctg ttgataccac aaatacacct acaggaaaag
    15301 ccaataacag acgagtagaa tttataaaac tttaaataaa aaatcaataa atcatttcac
    15361 taaaagcaaa aaacatgaaa agaataatga tagctgcaat atgctttatt tcattgcagg
    15421 ctgtaaaagc acagattgaa ggaataactg cctttgaaca accttctaca agtaaaatta
    15481 ctcctaagct aaaagtttct attaatacaa aacaggcaaa tagtattgac gtatctcagg
    15541 acaataaagc gtttgcaaat tttactacac agcaattagt cgatggatgg gaaagaacca
    15601 aaacatctgt gtactcatca tcaacaaaag acgcatttgg tactattgca gtttcaataa
    15661 tatatttaaa agtcaaaaga ttacccgata attcaggaat gtgggttgta cggcaagcag
    15721 gcttactcaa tccattaaca aaaaaaacgg attatagtac tagagacagt acgctgtttc
    15781 ctaatccatt ctttaaaaaa accacaaaac ctattcctgg aactaaatta gaatggcatc
    15841 aagaatttac tatttccaac att
//
LOCUS       DAOQPK010000023        15696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_1896_length_15696_cov_4.462247, whole genome shotgun sequence.
ACCESSION   DAOQPK010000023 DAOQPK010000000
VERSION     DAOQPK010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 15696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15696
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1896_length_15696_cov_4.462247"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="PLU36_02130"
     CDS             complement(<1..873)
                     /locus_tag="PLU36_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HMZ45578.1"
                     /translation="MQQQRYYSLDVFRGATVALMILVNNPGSWASIFPPLEHAKWNGC
                     TPTDLVFPFFLFAVGNAMAFVMPKFEQKGSKYFLIKVIKRSILIFGIGLLLNWSPFVQ
                     WSGNELVFKPWSYLHTNANGTSYEDGVRLLGVLQRIAICYLIASLIVYYFKPKGAMVA
                     AIIILFAYWLISLLAGNSADPFSITGHFARNIDIAVLTKYHMLHIDKIDGKVFHFDYE
                     GLASTTAAIAQVIMGYLIGYYIQQKGKTYEMLSNLLVAAILFMAAGYCWDMVFPINKK
                     IWSSSYTIYTTGLAI"
     gene            1053..2246
                     /locus_tag="PLU36_02135"
     CDS             1053..2246
                     /locus_tag="PLU36_02135"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HMZ45579.1"
                     /translation="MKTCYIIDAVRTPIGKYAGKLSNTRPDDLLALVIKALVIRNPNI
                     DTNKIEDVIAGAANQSGEDNRDVARMAALLAGLPLTVGGNTVNRLCASGLQAIMDASR
                     AIMCGDGLLYIAGGTESMTRAPFVTAKADTAFNRKTETYDTTIGWRFINKKLADLYYP
                     FSMGETAENVAKQYGISREEQDLFAFQSQEKYAAALKANIWQEEIIPVEIFENKQAVL
                     FTKDEHPRQSSLEKLATLKPAFDKNGTVTAGNSSGINDGASAILLASEEAVKLFNLQP
                     LARVVSMATAGVDPSIMGIGPVPATQKALQRAGLSTNHLDLIELNEAFASQSIACIKE
                     LNLDTSKININGGAIALGHPLGCSGSRITTTLLHQMKKIKSKYGLATMCVGVGQGAAI
                     VYEGL"
     gene            2580..4994
                     /locus_tag="PLU36_02140"
     CDS             2580..4994
                     /locus_tag="PLU36_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ45580.1"
                     /translation="MKLKIFTYLIIFFLLHTTVKSQNSIIGVVQDANNKTNIEGAIVY
                     IAEIKMKTTTNKSGRFVLRNIPKGSYSLKVNATGYQTINKKIELSSDTLFNFFLEQNF
                     TALNEVVVTAVSRSTQVKLSPVIIKPVDIAQLNQNSSTNLIDALKNIPGVNQITTGSG
                     ISKPTIRGLGYNRVITLYNGIKQEGQQWGDEHGIEIDEYSVSRVEIVKGPGSLMYGSD
                     AIAGVLNFITPKIKSLKHTTTQLISNYQSNNNLIGNSFSHAGSTKSFHWLGRVSNKLA
                     GNYQNKTDGKVYNSGFKELNANIFLGIHKNWGYTHFNISTYNIELNIVDGDRDSLGNF
                     VYDFVNNNRTITKKTVTSNILKGYKRGIPYQIVNHFRVMSNSFFQLSKGSINMDIGYQ
                     NNRRKEFGNALYENRKDQFFDLHTINYSARFNFLENNNWATSIGINGMQQNNFNKGLE
                     FLIPQYNLFDIGGYIITQKLFNKLTLAGGLRFDLRKLATKKLVLDSLKRPVNYEDNTT
                     IIKFNNLNKVFNNFSGSVGISYQINKNETLKLNISRGFRAPSMAELTSNGQHEGTFRY
                     EFGNAQLQSEVSHQIDIAYFNNTSEFTLECTPFINFVSNYIYTYKLVSVFGGDSIYNP
                     IKPFPSIKYTQGNAVLFGGELYFNLHPTAIKWLNIESAFSYVRATQNKQPDSTKFLPL
                     IPAPKLNCNVKLQCKQVGKLFTNAYFKIGFDYFLEQNKYYKANFSETLTPAYWLLNFG
                     FGGNLKLFNKKDMLSLFFSADNFLDVSYQSHLNRLKYAPQNKLTGKNGVFNMGRNFCI
                     KTIFNF"
     gene            5070..5756
                     /locus_tag="PLU36_02145"
     CDS             5070..5756
                     /locus_tag="PLU36_02145"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HMZ45581.1"
                     /translation="MKARNLIILILVVLFADQALKFYIKLNYYTGEEHKVIGNWFRLH
                     FVENEGMAWGWKFGGGFGKIALTLFRLAAVIWGTFLLRDFIKKKYHKWFIVCAALIYI
                     GALGNLIDSMFYGLIFDISYDEYHTHFYGQVAKAFTGVHYGSFLHGKVVDMFYFPLLK
                     GNFPEWMPFWGGEEFEFFRPVFNIADASISVGIITILIFQNKFFNQKQVANTNTVETS
                     TEVNDTTQVL"
     gene            5786..6406
                     /locus_tag="PLU36_02150"
     CDS             5786..6406
                     /locus_tag="PLU36_02150"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HMZ45582.1"
                     /translation="MIQKLYFHNFIKASIISLVAAIILFYFSFTLGKHPFFLLLNNNL
                     GIVADYFFKFYTHFGDGTIWAILLFLFIKFNKKYIPFLIAAFILCTLLVQVCKYVILP
                     NEPRPIAAIQPTSLIHTVQGVEPHTISTFPSGHTAAAFCFFFIASLFIHKKWIIPVGF
                     LYAILVGYSRVYLAQHFPFDVAAGIVTAIIAISFSIFIQKIFEKKK"
     gene            complement(6425..7693)
                     /gene="ricT"
                     /locus_tag="PLU36_02155"
     CDS             complement(6425..7693)
                     /gene="ricT"
                     /locus_tag="PLU36_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HMZ45583.1"
                     /translation="MGCSSCSKSNGKPNGCQSNGGCSSGSCNRLNVHDWLVNLPIDDY
                     ESSCKIVEVSFKQGSRKEFYRNPTLQFFEKGEYVTVEGVNGFDVGEVSLSGELVRVQM
                     KKKGVEEFNPEIKKILRRSSDRDIEIFKINKDREPSVQAKSRELAIIHKLQMKISEVE
                     IQADGKKATFFYTADDRVDFRELIKQYASEFKLKVEMRQIGIRQEAAKVGGIGSCGRE
                     LCCSTWLHDFKSVNTTAARYQNLSINQTKLSGQCGRLKCCLNYELDTYLDALQHFPDN
                     ADTFETTKGRAFLIKKDIFKNLMWYVLEGGNKHYPLTIQRVKEIKKLNAQGNKPDDLE
                     AVEVTSSKPKEVEPQFVDVVGQISLRSLEKSDSRRRNQQRDQRVDKRGNERNLKINTD
                     RNRNHLNTNQPKQNQQPNSGNNQPPPRKRN"
     gene            8082..8867
                     /locus_tag="PLU36_02160"
     CDS             8082..8867
                     /locus_tag="PLU36_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HMZ45584.1"
                     /translation="MIEQINEGYVKVESEHGIVTIEFHHPQSNSLPGKILDALAKEIH
                     AAGINPNTRVIILQSTGTKVFCSGASFDELASIKTEEEGLKFFSGFAKVINAMRKTPQ
                     LIIARVHGKCVGGGVGLAAAADYAIACDGAEVKLSELAVGIGPFVVGPAVERKLGLSA
                     FSQLAIDANLWRPADWARRKGLFAELHNEVTGMDDSIARLSLNLSKASPAAMHELKKI
                     FWQGTEHWDELLKERAAISGKLILSDFSKNFIAKFKEKQKENK"
     gene            9079..10593
                     /locus_tag="PLU36_02165"
     CDS             9079..10593
                     /locus_tag="PLU36_02165"
                     /inference="COORDINATES: protein motif:HMM:NF014344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HMZ45585.1"
                     /translation="MSERNLYVDSELGTLRKILIHSPDGGIGKIIPGTFADNLYDDIV
                     HLKKMQKEYNHYVKLLLYYLDPEKAKYISEYEKNQTSSNAGFCFVPGRKEYFNSNKVL
                     EAQTLLEEIVKDEKVKLRLIAAVCSYEESSFHIQHKLENINNPALLAKIFITGIFPAE
                     ENGTKEDQYIFPPIPNFIFTRDNAIMMKDHILLSRMATTARKRESLISKFLALYFLFR
                     DNPEKVIEVIEESDYFLYEEEERKQRIITIEGGDIMMIHPTHLLVGCSERTSASAINE
                     IIHTIFNNSHLEIEKISVVKIPKKRAQMHIDTIFTQVKRNVWVLYGRYSDTILKAEED
                     TKKSYLKKLSHVPKKTELEEVEIFQFYKNKKEPYAKNKDYSISNAPAGLESLLRHISE
                     VDFGVKPEDVKIIYSGNNEFPHDEREQWTDSSNVVALKEGVVISYDRNDKTTETFKNA
                     GFNIVTTTQVFEMFEKGIDPHSLEDTVILIPSAELSRARGGSHCMSMPLLRDKW"
     gene            10614..11525
                     /locus_tag="PLU36_02170"
     CDS             10614..11525
                     /locus_tag="PLU36_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HMZ45586.1"
                     /translation="MQTTQHLLMIKPVHFTYNAQTAVNNAFQNAAADSNTQQNALNEF
                     NNFVELLMQNGIDVTVIEDTLQPHTPDSIFPNNWISFHANGTILLYPMYAENRRQERK
                     PHVLENISKKFSISTTIDLTNYEKSNLFLEGTGSMVLDREKNIAYACISPRTDATILE
                     EFCNKMHFTPMLFKANDSNNSPIYHTNVMMCVADKYVVICLDAITDHQERSTVIHSIE
                     SSGKQIVPITLQQMNKFAGNMLQVNNQKGEKFLIMSSQAYQALTPQQINQLQSYNHIL
                     HSNLTTIETNGGGSARCMMAEVFLPLK"
     gene            11620..12468
                     /locus_tag="PLU36_02175"
     CDS             11620..12468
                     /locus_tag="PLU36_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HMZ45587.1"
                     /translation="MNTTTYQIKLDHFEGPFDLLLFFIERDELDIYNIPITKITNDFL
                     NYIHSQEKLNIELSSEFILFVSTLMRIKAKLLIPRKELDADGKEIDPRQELVDKILEY
                     KKFKEASAKMADMEALRMLMVKRGNLQKELNEIGEQAGEGTEIQNITLFKLMKSFEKV
                     MQRLNERQNKPVHTVVRYNYTMEGSRDYMLHFIQKEKTVSFEKVFDVCESRVHAIFLF
                     LSMLELTQQRFMKLLIGEGRNNFIIEWNENREQEIQEEGLDINELNNMPTSFPDSIQD
                     DIATLN"
     gene            12483..13301
                     /locus_tag="PLU36_02180"
     CDS             12483..13301
                     /locus_tag="PLU36_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ45588.1"
                     /translation="MLFNNKTVFISGASRGIGKAIALKLASYGANIVIAAKSTTENSK
                     LGGTIYTAAKEIEAAGGKALAVQTDVRNEEQIANAVKQTIETFGGVDIVINNASAIQL
                     TNTAQTEAKRFDLMHSVNVRGSFLVVQHFLPYLQKGNNPHILTLSPPVNLISKWLSPH
                     IAYTMSKFNMSMMALAWAEEFKNFGIASNALWPQTTIDTAAVRNLLGGETLANMSRTP
                     QIIADAAFYILSKQNLEYTGNTFIDEIVLAKEGITNFDKYAVVPGAKLYTDLFL"
     gene            13364..14239
                     /locus_tag="PLU36_02185"
     CDS             13364..14239
                     /locus_tag="PLU36_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HMZ45589.1"
                     /translation="MQLHPVDIVQNISAEEFKKKYYLTSKPLVIKSLSQQWSAYSKWN
                     WDYFKTLVGSQKVALYNNIKSNAYTPINTADDYKTFGEYIDMIKAGPAGWRIFLFNIF
                     DHAPQLINDFTWPEHLTKGFVKKYPMLFTGGATSITHMHFDIDLSHIFHTQFIGRKRV
                     LLFPFKEQYKLYRKPFEVLSFVDFSNYYDEANSKIDYTKFPILKNAQGMEVILEHGDT
                     LFMPAGYWHHMEYLDSGFAMSLRALQPSITGKLAGVWNLFGMRNIDTLLKKTIPQPWY
                     NWKQKKAYARAAKEI"
     gene            14335..>15696
                     /locus_tag="PLU36_02190"
     CDS             14335..>15696
                     /locus_tag="PLU36_02190"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMZ45590.1"
                     /translation="MFTKLKLLIVACFCIAIINAQETFPVNGTKDVRINCYAFTNATI
                     IQNATTTLQNATLVIKQGKIVGINIPVPKEAVVVDCKGKFIYPSFIDLFSEYGLKPVA
                     NTGFRGFGQPRQFESKTKGAYNWNQAIKPEINAFEKFEGNEKLAKELRAIGFGTVVTH
                     EKDGIARGTGALVTLGDDKENFELVKEKVGAFYSFNKGSSTQDYPGSLMGVIALIRQT
                     YIDAAWYKNKPTTEGTNISLQAWNNNLSLPQIFDIGNDKYNALRANKIAKEFGATYIL
                     NGSNDMYQRLDEIKAMNSSIILPLNFPAPMDVEDPNDARMVAYADLKHWEMAPYQPAM
                     FEKENINFSLTTNGLTATNDFLINLRKAIHNGLSEKTALDALTRLPATWINAYDITGS
                     LEPGKLANFFITNTNIFSDSATIFQNWVQGKKYIVKENGWNDYNGTYVFTVTTKGKTN
                     FYEA"
BASE COUNT         5688 a         2304 c         2538 g         5166 t
ORIGIN      
        1 aatggcaagc cctgttgtat aaatggtata gctactgctc catatttttt tgttgatagg
       61 aaaaaccata tcccaacaat agccagctgc cataaataaa attgcagcta ccagtaaatt
      121 gctaagcatt tcgtaagttt ttcctttttg ttgaatataa tatccaatta aatatcccat
      181 aataacctgt gctattgcag cagttgtact tgccagccct tcataatcaa aatgaaaaac
      241 tttgccatca attttatcaa tatgtaacat gtggtatttg gttaatacag caatgtcaat
      301 atttctggca aaatgccctg taatagaaaa agggtctgca gaattaccag ctaataaact
      361 tattaaccag taagcaaata atataataat tgcagcaacc attgcacctt tgggtttaaa
      421 gtaatacaca attaacgatg caattaaata gcaaatagca atacgttgca acacacctaa
      481 aagtcttaca ccatcttcat agcttgtgcc atttgcattg gtatgtaaat aagaccatgg
      541 cttaaaaaca agttcgttgc cgctccattg tacaaatggg ctccaattta ataataaccc
      601 aattccaaaa attaaaatac tacgcttaat cacttttatt aaaaaatact ttgagccttt
      661 ttgttcaaac tttggcatta caaaggccat tgcattacca actgcaaata aaaaaaatgg
      721 aaaaaccaaa tctgtaggtg tacagccatt ccatttggca tgttctaaag gaggaaaaat
      781 agaagcccag ctacccggat tgtttactaa tatcattaaa gctacagttg ctcctctaaa
      841 aacatctaat gaatagtatc gttgctgttg catagataat tttatttata gcgtaacgaa
      901 tgtatgcaat ttgattagta aataaaaaga caatctctga acatttttat tctcactgtt
      961 tcataaaaca aattaataag taatcaaaac ttgttaaata cgttacgcta cttatacaat
     1021 atttttcttt cctttgctac ttaaatttta atatgaaaac ttgttatata atagatgctg
     1081 taagaacacc aataggtaaa tatgctggaa aattgagtaa tacaagaccc gatgatttat
     1141 tagcacttgt tattaaagca ttggttatta gaaaccccaa tattgatact aataaaatag
     1201 aagatgtaat tgcaggagct gcaaaccaaa gtggagaaga taatagagat gttgcaagaa
     1261 tggcagcatt gttagctggt ttgcctttaa cagttggtgg caatactgta aacaggttgt
     1321 gtgcaagcgg cttgcaagca ataatggatg catctagagc catcatgtgt ggagatggtt
     1381 tactgtatat agctggtggt acagaaagta tgacaagggc accatttgta actgctaaag
     1441 cagatactgc ttttaataga aaaacagaaa cctatgatac tactattggt tggcgtttta
     1501 taaataaaaa attagccgat ttatactacc cttttagtat gggcgaaacg gcagagaatg
     1561 ttgctaaaca atatggcata agtagagaag agcaggattt atttgcattt caatcgcaag
     1621 aaaaatatgc tgctgcatta aaagcaaata tttggcaaga agaaattata cctgtagaaa
     1681 tttttgaaaa taaacaagcc gttttgttta caaaagatga gcatcctagg caatctagtt
     1741 tagaaaaatt agcaacctta aaacctgcat ttgataaaaa tggaacagtt actgcaggaa
     1801 actcatcagg tataaacgat ggagcatctg ctatattatt ggctagcgaa gaagctgtaa
     1861 aactttttaa tttacaacca ttagcaagag tagttagtat ggcaactgct ggggttgatc
     1921 cttctataat gggtattggg ccagtgcctg ctacacaaaa agcattacaa cgtgcagggc
     1981 tttccacaaa ccatttagat ttaatagagt taaatgaggc atttgcttct caatcaatag
     2041 cctgtattaa agagttaaat ttagacacta gtaaaataaa cataaatggt ggtgcaattg
     2101 ctttaggcca tccattaggt tgcagtggta gtagaattac tacaacactt cttcatcaaa
     2161 tgaaaaaaat aaaaagtaaa tatggtttag ctactatgtg tgttggtgta ggacaaggtg
     2221 cagcaattgt ttatgaagga ctataaaggc tttgaataaa aattaaaaat atataatttg
     2281 ttttattaag tattaaaaat cttgatgtaa tattgtttaa aatattcagc ttatagcttc
     2341 aattttacgc actacttact gttttagtat tcttattttt aaatattttt ctatttaaaa
     2401 attttcaatt taaactattc attataacac tagctaagtt ctatttggct actcgttata
     2461 tgttttcaag aatacatttt taaaggttaa actgttcatt acggtgtaat gaatgtattt
     2521 aattatttca atactataca agcactttta ataaaaaaca ttaaactttt tatataaata
     2581 tgaaattaaa aatttttact tatttaatta ttttctttct actacataca accgttaaaa
     2641 gccaaaattc tattattggt gttgtacaag atgcaaacaa caaaacaaac attgaaggag
     2701 caattgtgta cattgctgaa ataaaaatga aaacaacaac aaataaatct ggaaggtttg
     2761 tgctaagaaa tatacctaaa ggtagttata gtttaaaagt aaatgctaca gggtatcaaa
     2821 caataaataa aaaaattgaa ttaagcagcg atactttatt taattttttc ttagaacaaa
     2881 attttacagc attaaatgaa gtggttgtta cagcagtgtc tagaagtact caggttaagt
     2941 taagtccagt aattattaag cctgtagata ttgctcaact aaatcaaaat agttccacta
     3001 atttaattga tgcattaaaa aatattccag gtgttaatca aattacaaca ggttctggaa
     3061 tttcaaaacc aactatacgt ggcttgggtt ataatagggt aataactttg tacaatggta
     3121 ttaagcaaga ggggcaacaa tggggagatg aacatggaat tgagattgac gaatattctg
     3181 taagtagggt tgagattgta aaaggccctg gtagtttaat gtatggctca gatgcaatag
     3241 ctggggtttt aaattttatt actcctaaaa taaaatcact taaacataca acaactcagt
     3301 taatatctaa ctatcaatcc aataataatt taataggaaa ttctttttcg catgcaggtt
     3361 caacaaaaag ttttcattgg ttagggagag taagcaataa gcttgctgga aattatcaaa
     3421 ataaaacaga tggtaaagta tataactccg gttttaaaga actaaatgca aatatttttt
     3481 tgggcattca taaaaattgg ggctatactc attttaatat cagtacttat aatattgaat
     3541 taaatatagt ggatggagat agagatagct taggaaattt tgtttatgat tttgtaaaca
     3601 acaatagaac aataacaaaa aaaacggtta ctagtaatat tttaaaaggc tataaaagag
     3661 gaatcccata ccaaattgta aatcacttta gagtaatgag taatagtttt tttcagctta
     3721 gtaaaggttc cataaatatg gatattggat atcaaaataa tagaagaaaa gaatttggaa
     3781 atgcattata tgaaaatagg aaagatcaat tctttgattt gcatacaatc aattattcgg
     3841 caagatttaa ttttttagaa aacaataatt gggctacatc aattggtatt aatggaatgc
     3901 aacaaaacaa ctttaataaa ggattagagt ttttaatacc tcaatacaat ttatttgata
     3961 taggtggcta tatcattact caaaaactat ttaataaact aacattagca ggtggtttaa
     4021 ggtttgattt aagaaagtta gcaacaaaga aattggtatt agatagttta aaaagacctg
     4081 taaactatga ggataataca acaatcataa agtttaataa tttaaataaa gtattcaata
     4141 atttttcagg aagtgttggc ataagctatc aaataaataa aaatgaaact ctaaagctta
     4201 atatttctag gggatttaga gcaccaagta tggcagagct tacatcaaat gggcaacatg
     4261 aaggaacatt taggtatgag tttggtaatg cacaacttca atcagaagtt agccatcaaa
     4321 tagatattgc ctattttaac aacacatcag aatttacttt agagtgtacg ccatttataa
     4381 attttgtatc caactatatt tatacttaca aattagtgag tgtttttgga ggagattcca
     4441 tttataatcc aataaaacct tttccatcaa ttaaatatac acaaggcaat gctgtattgt
     4501 ttggtggcga attatatttt aatctccacc caactgctat aaagtggttg aatattgaaa
     4561 gtgctttttc ttatgttaga gcaacccaaa ataaacagcc agacagcaca aaattcctgc
     4621 ctcttatacc tgcaccaaaa cttaattgca atgtaaaact tcagtgtaaa caagtaggta
     4681 aattgttcac caatgcctat tttaaaattg ggtttgatta ttttttagaa cagaataaat
     4741 attataaagc taatttttct gaaaccctaa caccagctta ttggttatta aactttggct
     4801 ttggagggaa tttgaaattg tttaataaaa aagatatgct aagtttattt tttagtgccg
     4861 ataatttttt agatgtttct tatcagagtc atttaaacag attaaaatat gcacctcaaa
     4921 ataagctaac tggcaaaaac ggtgttttta atatgggcag aaacttttgt ataaaaacaa
     4981 tttttaattt ttaatgttaa tacaatattc acttttatca gtagccattt ctcttatctt
     5041 tgccccactt aatttttaag catactatag tgaaagcaag aaatttaatt attttaattt
     5101 tggttgtttt gtttgccgac caagcattaa agttttatat aaaacttaat tattatacag
     5161 gagaggaaca taaagtaatt ggtaattggt ttaggctgca ttttgttgaa aatgaaggaa
     5221 tggcttgggg ttggaaattt ggtggcggtt ttggtaaaat tgctttaacc ttatttaggc
     5281 tggctgcggt aatttggggt acttttttat taagagattt tatcaaaaaa aaataccaca
     5341 aatggtttat tgtttgtgct gcattaattt atattggtgc attgggtaac ttaatagata
     5401 gcatgtttta tgggttaata tttgatataa gctacgatga gtaccatact catttttatg
     5461 gccaggttgc aaaggcattt actggtgtac actatggaag ttttttacat ggtaaggtgg
     5521 tagatatgtt ttactttcca ttattaaaag gtaattttcc ggaatggatg ccattttggg
     5581 gaggagagga atttgagttt tttagaccag tttttaatat tgcagatgct tctatatctg
     5641 ttggtattat taccattctt atttttcaga ataaattttt taatcaaaag caagttgcaa
     5701 ataccaacac agttgaaacc tcaaccgaag taaatgatac aacacaagtg ttgtaattat
     5761 ttattctttt tgcaataata attttatgat acaaaaatta tacttccaca attttattaa
     5821 agcaagtata attagtttag tagctgctat tatacttttt tatttttcgt ttacgttggg
     5881 taaacatccc ttttttctat tactaaataa taatttaggt atagttgccg attatttttt
     5941 taaattttat acccactttg gtgatggtac tatttgggct attcttttat tcctttttat
     6001 aaaattcaat aaaaaataca ttcctttttt aattgctgca tttatactgt gtaccttatt
     6061 agttcaggtt tgtaaatatg taattttacc taacgaacca aggcccatag ctgctattca
     6121 gccaacaagc ctaatacata ctgttcaagg agtagagcct catactattt caacatttcc
     6181 atctgggcat actgctgctg ctttttgctt cttttttata gcttcattgt ttatccataa
     6241 aaaatggata attcctgtag gttttttgta tgctattttg gttgggtatt caagagttta
     6301 tttggcccaa cactttccat ttgatgtagc tgctggtatt gttacagcca taatcgctat
     6361 aagtttttcc atctttattc aaaaaatatt tgaaaaaaag aagtaaccat aacaatctat
     6421 aaaattaatt tctctttcgt ggcggtggtt gattgttacc actatttggc tgctgatttt
     6481 gttttggctg atttgtattt agatggtttc tatttctgtc agtatttatt tttaaatttc
     6541 tttcattacc tctcttatct accctttgat ctctttgttg atttcttctt ctgctatccg
     6601 atttttctaa actacgcaaa ctaatttgcc caactacatc tacaaattgt ggttccactt
     6661 ctttaggttt tgaacttgtt acttcaactg cctctaaatc atctggttta ttgccttgag
     6721 cgtttaattt cttaatttct ttaacccttt gtatggttaa gggataatgt ttgttaccac
     6781 cttctaacac ataccacata aggtttttaa aaatatcttt tttaattaaa aatgccctgc
     6841 cttttgtagt ttcaaaagta tctgcattat ccggaaaatg ttgtaaagca tctaaataag
     6901 tatctagctc gtaatttaaa caacatttta gcctgccaca ttgcccacta agttttgttt
     6961 gattaatact taagttttga taacgtgccg ctgtagtatt tacactttta aaatcatgaa
     7021 gccaagtgct acagcacaac tctcttccac aacttccaat accgccaact ttagctgctt
     7081 cttgtctaat accaatttgc ctcatttcaa cttttaactt aaactctgag gcgtattgtt
     7141 taattaattc tctaaaatca acacggtcat cggcagtata aaaaaaagtt gcttttttgc
     7201 catcagcctg aatttcaacc tcactaattt tcatttgtaa cttatgtata atggccaatt
     7261 ctctgctttt tgcctgaacg ctaggctctc tatctttatt tattttaaaa atttcaatat
     7321 ctctatcgct acttcttcgt aatatttttt ttatttctgg gttaaattct tctactccct
     7381 tttttttcat ttgtactctt accaattcac cacttaacga aacttctcca acatcaaagc
     7441 cattaactcc ctctacggtt acatattctc ctttttcaaa aaattgtaaa gttgggtttc
     7501 tgtaaaattc ttttctacta ccttgcttaa aactaacttc tactatttta caactacttt
     7561 cataatcgtc aattggtaaa tttactagcc agtcgtgtac attcaatcta ttacagctac
     7621 cagagctgca acctccattg ctttgacatc cattgggttt cccattgctt ttgctacaag
     7681 aactgcatcc cattatatat acagttaaaa aatgaataaa tgttttggag aaattttaag
     7741 aaaatcaagc agattggtta cattaccctt ctgtaaggct attattatat atgaatttat
     7801 aaaaataaaa tagtaagctt ttagtttaat tagtttatat aataaataag gtaatttgta
     7861 gttctactta aaaatttata cttcaaaatc tgtttctaca ttaagaattt atttcttaaa
     7921 atttctctac ctttcaaaaa taagagtttt attttaaatg aaaactatct tacacctact
     7981 gggtactaat taacaaaact taaattaata aaaaactatc ttagtaacct catttaccaa
     8041 ataaatttta aaaaatatct tttcttataa aaaaataaag tatgattgag caaattaatg
     8101 aaggatatgt aaaagttgaa agtgagcatg gtattgtaac aatagagttt catcacccac
     8161 aaagcaactc attaccaggt aaaattttag atgctttggc taaagaaata catgcagcag
     8221 gtataaaccc taacactaga gttattattt tacaaagtac cggaactaag gttttttgca
     8281 gtggtgcaag ttttgatgaa ctagcctcca ttaaaacaga agaagaggga cttaaattct
     8341 tttctggctt tgcaaaagtt ataaatgcca tgcgtaaaac accacagcta attattgcaa
     8401 gagtgcatgg taagtgtgtg ggtggtggtg ttgggcttgc agcagcagca gattatgcca
     8461 ttgcttgcga tggtgcagaa gtaaaactaa gtgagcttgc agtaggcatt ggaccttttg
     8521 ttgttggccc tgcagttgaa agaaaattag gcttaagtgc cttttcacaa ttagcaatag
     8581 atgctaattt gtggaggcca gcagattggg caagaagaaa aggactattt gcagaattgc
     8641 ataatgaggt tacaggaatg gatgatagta ttgctagatt atctttaaat ttatcaaaag
     8701 ccagccctgc cgcaatgcat gaattgaaaa aaatattttg gcaaggaaca gaacattggg
     8761 acgaattgct taaggaaagg gcagcaatta gcggaaaatt gattttaagc gattttagca
     8821 aaaattttat tgcaaaattt aaagaaaagc aaaaagaaaa taaatagaat aatatttatg
     8881 taatagcatc agctatctac ccttacattt ttttcattgg ggtaaatgcc gtactttgca
     8941 agcaaacaga aagctatatt ctggatagtt agcttatatt tattttgtat ctaatacagt
     9001 aatcaaaaat aggtgcgatt gttttattag ctattcaata caatcggtaa ctgaatattt
     9061 tctgtaaaaa tttattatat gtcagagcgt aatttatatg tggactcaga attgggtacc
     9121 cttagaaaaa ttttaattca cagcccagat gggggtattg gaaaaattat tccaggcact
     9181 tttgccgata atttatacga tgatattgtg catctaaaaa aaatgcaaaa agagtataac
     9241 cattacgtta aacttttact gtattattta gatcctgaaa aggcaaaata tattagtgag
     9301 tatgaaaaaa atcaaactag tagcaatgct ggcttttgct ttgtaccagg tagaaaagaa
     9361 tattttaact ctaacaaggt tttagaagca caaacacttt tagaagaaat agtgaaagat
     9421 gaaaaggtaa aactacgatt aatagcagca gtatgctctt acgaagaatc tagctttcat
     9481 attcagcata aactagaaaa cattaataac ccagcattat tagctaaaat atttattaca
     9541 ggcatttttc ctgcagaaga aaatggaacc aaagaggatc aatatatttt tccacccatt
     9601 cctaatttta tatttacaag agataatgct ataatgatga aagaccatat tttactgagc
     9661 agaatggcaa caacagctcg taaaagagag tcattaattt ctaaattttt agcactttat
     9721 tttttattta gagataatcc agaaaaggta attgaagtaa ttgaagaaag tgattatttt
     9781 ttgtatgaag aagaagaacg taagcaaaga ataattacta ttgagggtgg cgatattatg
     9841 atgattcacc caacacattt attggtaggt tgtagcgaaa gaacatcggc tagtgcaatt
     9901 aatgaaatta ttcataccat tttcaataac tctcatctag aaattgaaaa aatttcggta
     9961 gtgaaaattc ctaaaaaaag agctcaaatg catatagaca ccatttttac acaagtaaaa
    10021 agaaatgttt gggttttata tggcagatat tctgatacta tcttaaaagc agaagaagat
    10081 accaagaaat cttatcttaa aaaattatcg catgtgccta aaaaaacaga gctagaagaa
    10141 gtagaaattt ttcaattcta taaaaacaaa aaagaacctt atgctaaaaa taaagattat
    10201 agcataagta atgcccctgc agggttagag tcattattaa ggcatataag tgaggtagat
    10261 tttggtgtaa agcccgaaga tgttaaaata atttatagcg gcaacaacga atttcctcat
    10321 gatgaaagag aacaatggac agactctagc aatgtagtag ccttaaaaga aggggttgta
    10381 attagttatg atagaaatga taaaacaaca gaaacgttta aaaatgcagg gtttaatatt
    10441 gtaactacaa cccaagtgtt cgaaatgttt gaaaaaggta ttgacccaca tagtttagaa
    10501 gatacagtaa tactaatacc atctgcagag ttatctagag ccagaggagg atctcattgc
    10561 atgagtatgc ctttattaag agataaatgg taaactattt aataaaaaat tttatgcaaa
    10621 ctacacagca tttgttgatg ataaagcctg tgcattttac ttataatgca caaacagcag
    10681 taaataatgc atttcaaaat gcagcagcag atagcaacac acagcaaaat gcactgaatg
    10741 agtttaataa ttttgtagag ttattaatgc agaacggtat tgatgtaact gtaatagaag
    10801 atactttaca accacataca cccgattcta tttttcctaa taactggatt tcttttcatg
    10861 ccaatggcac catcctgtta taccccatgt atgccgaaaa tagaaggcaa gaaagaaaac
    10921 cacacgttct tgaaaatatt tctaaaaaat tcagtatttc tacaaccata gacttaacca
    10981 actacgaaaa aagtaacttg tttttagaag gcactgggag catggtttta gatagagaaa
    11041 aaaacatagc ttatgcctgt atttctccaa gaacagatgc cacaatttta gaagaatttt
    11101 gcaataaaat gcactttaca ccaatgttgt ttaaagctaa tgactcaaac aactcaccca
    11161 tttatcatac caatgtaatg atgtgtgtag ctgataaata tgtggtaatt tgtttagatg
    11221 caattactga ccatcaagag agaagtacgg ttattcactc tatagaaagc tctggtaaac
    11281 aaattgtacc aataacctta cagcaaatga acaaatttgc tggcaatatg ctacaagtta
    11341 ataatcaaaa aggagaaaag tttttgatta tgtcatcgca agcataccaa gccttaacgc
    11401 cacaacaaat aaatcaacta caaagctaca accatatatt acattccaac cttacaacta
    11461 ttgaaacaaa tggtggagga agtgcaagat gtatgatggc tgaagtattt cttcccttaa
    11521 aataaagggt ataaattatt ttttatagtg gataattaag ctgtgtaatt gctcttcata
    11581 tcttaattgt acgtttaatt tgcaataaat aaaattgctg tgaatacaac tacttaccaa
    11641 ataaaattag accactttga gggacctttc gatttgttat tattttttat tgaaagagat
    11701 gagttagata tttacaatat acctattaca aaaattacca acgatttttt aaattatata
    11761 cactctcagg aaaaattaaa tatagagtta agtagcgagt ttattttatt tgtttctacc
    11821 ctaatgagaa taaaagcaaa gctacttata ccacgtaaag agttagatgc agatggtaaa
    11881 gaaatagatc cacgacaaga gttagtagat aaaatattag agtataagaa gtttaaagaa
    11941 gcctccgcaa aaatggcaga tatggaagcc ttgagaatgt taatggtaaa aagaggcaac
    12001 ttgcaaaaag agttaaatga aataggagag caagcaggag aaggaacaga aattcaaaac
    12061 attactttat tcaaactaat gaaatctttt gaaaaagtaa tgcaacgcct caatgaaaga
    12121 caaaacaagc cagtacacac tgttgtaagg tataattata caatggaggg aagcagagat
    12181 tacatgttac attttatcca aaaagaaaaa acagtttcat tcgaaaaagt ttttgatgtt
    12241 tgtgagagta gagtgcatgc catctttctt tttttatcta tgttagaact tacacaacaa
    12301 cgattcatga aattattaat tggcgagggg agaaataatt ttattattga atggaatgaa
    12361 aacagagaac aagaaataca ggaagaaggg ttagatataa atgaattaaa caatatgcca
    12421 acttcttttc ctgattccat acaagatgat attgcaacat taaactaata attttttacc
    12481 gcatgttatt taacaataaa acagttttta tttcaggagc ttcaagaggt attggtaaag
    12541 ccatagcatt aaagcttgca agttatggag ctaatattgt tattgcggca aaaagcacta
    12601 ctgaaaattc aaaactagga ggtacaattt atactgctgc aaaagaaata gaagctgcag
    12661 gtggcaaagc tttagcagta caaacagatg ttagaaatga agagcaaata gccaatgctg
    12721 taaaacaaac aatagaaaca tttggaggtg ttgatattgt aattaacaat gcttctgcaa
    12781 ttcaactaac caatacagca caaacagaag cgaaacgctt tgacttaatg catagtgtaa
    12841 atgtacgtgg tagcttttta gtagtgcagc attttttacc ctatctacaa aaaggaaaca
    12901 atccacacat acttacactt tcaccaccag taaatttaat aagtaaatgg cttagccctc
    12961 atattgccta tactatgagc aaatttaata tgagtatgat ggcattagct tgggcagaag
    13021 agtttaaaaa ttttggcatt gcaagcaacg cactatggcc acaaacaacc atagatactg
    13081 ctgcagtaag aaatttatta ggtggcgaaa ctttagctaa catgagtcgc actccacaaa
    13141 ttattgcaga cgcagctttt tatattttat ctaaacaaaa tcttgaatat actggcaaca
    13201 catttattga tgaaattgta cttgccaaag aaggtatcac caactttgat aaatatgctg
    13261 ttgtaccagg tgctaaactt tatactgatt tatttttata aaagttttaa ataattatat
    13321 ttttcttcaa taaattctta tttatttttg aattttattt tatatgcaat tacatccagt
    13381 agatattgtt caaaatattt cggcagaaga gtttaaaaaa aaatattatc ttacttcaaa
    13441 gccattagta ataaaaagtt tatctcagca atggtctgct tatagtaaat ggaattggga
    13501 ttattttaaa acattggtag gcagtcaaaa agtggctttg tataataata ttaaaagcaa
    13561 tgcatacaca cctatcaata cagcagatga ttacaaaacc tttggagagt atattgatat
    13621 gataaaagca gggcctgcag gatggagaat ttttttattt aatatttttg atcatgcccc
    13681 acaactaata aatgatttta cttggccaga gcatttaaca aaaggctttg taaaaaaata
    13741 tccaatgtta ttcactggtg gtgcaacatc tataactcac atgcattttg atatagattt
    13801 aagccatatt tttcatacac agtttattgg tagaaaaaga gtattattat ttccgtttaa
    13861 agagcaatat aaattatatc gtaaaccatt tgaagtttta agttttgttg attttagtaa
    13921 ctattatgat gaagcaaata gtaaaataga ttatactaaa tttcctattc taaaaaatgc
    13981 acaaggaatg gaagtaattt tagaacatgg agatactcta tttatgcctg ctggttattg
    14041 gcaccacatg gagtatttag atagtggatt tgctatgagt ttaagagcct tacaaccttc
    14101 tataacaggc aaacttgctg gtgtgtggaa tttatttggt atgagaaata tagatacgct
    14161 tttgaaaaaa accattccac agccatggta taactggaaa caaaaaaaag cctatgccag
    14221 agctgctaaa gaaatataaa acggttagca gctattttta tttttttaat tttatgaacg
    14281 gtataatttt ttgtactgtt ttatctaatt ttagtcctaa caaatatttt aaagatgttt
    14341 acaaaactaa aactgctaat tgtagcatgc ttttgtattg ctataataaa tgcccaagaa
    14401 acttttcctg taaacggaac caaagatgta agaataaatt gttatgcatt tactaatgca
    14461 actattattc aaaacgcaac cacaacttta caaaatgcaa cacttgtaat aaagcaagga
    14521 aagattgttg gtataaatat tcctgtacct aaagaggcag ttgtggtaga ttgtaaagga
    14581 aagtttatat atccgtcttt tatagattta ttttcggaat atggcctaaa acctgttgca
    14641 aatacagggt ttagaggttt tgggcaacct cggcagtttg aatctaaaac aaaaggagct
    14701 tataattgga atcaggcaat aaaaccagaa ataaatgctt ttgaaaaatt tgaaggaaac
    14761 gaaaaattag ctaaagagtt aagagcaatt ggttttggca cagttgtaac acacgaaaaa
    14821 gatggcattg cacgtggtac tggagcatta gttacacttg gcgatgataa agaaaatttt
    14881 gaattagtta aggaaaaagt aggagccttt tattctttta ataaaggaag ttctacacaa
    14941 gattatccag gctctttaat gggtgttatt gcacttattc gccaaacata tattgatgca
    15001 gcatggtata aaaacaaacc aaccaccgaa ggcacaaata ttagcttaca agcatggaac
    15061 aacaatttat ccctacctca aatttttgat attggaaatg ataaatacaa tgcattaaga
    15121 gcaaataaaa ttgcaaaaga gtttggagca acttatattt taaatggcag caatgatatg
    15181 taccaaaggt tagatgaaat taaagcaatg aattcatcta taatattacc actaaatttt
    15241 cctgcaccaa tggatgtaga agaccctaat gatgcaagaa tggttgctta tgccgattta
    15301 aaacattggg aaatggcacc ttatcaacca gcaatgtttg aaaaagaaaa tattaatttc
    15361 tctctaacca caaatggctt aacagcaaca aacgattttt taattaactt aagaaaagca
    15421 attcataatg gcttatctga aaaaacagca ttagatgctt taacacgttt accagcaaca
    15481 tggataaatg cttatgacat aacaggaagt ttagaacctg gaaaattggc taactttttt
    15541 attaccaaca caaatatttt ttccgattct gcaactattt ttcaaaactg ggttcaagga
    15601 aaaaaatata ttgtaaaaga aaatggttgg aatgattata acggaactta tgtatttaca
    15661 gttaccacaa aaggcaaaac aaatttttat gaagct
//
LOCUS       DAOQPK010000024        15607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_1907_length_15607_cov_4.418274, whole genome shotgun sequence.
ACCESSION   DAOQPK010000024 DAOQPK010000000
VERSION     DAOQPK010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 15607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15607
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1907_length_15607_cov_4.418274"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="PLU36_02195"
     CDS             complement(<1..622)
                     /locus_tag="PLU36_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HMZ45591.1"
                     /translation="MLVKTFGSAVYGVEAISITIEVNVSGGDLKYYMVGLPDNAVKES
                     LQRIESAIKTNQLSFPRTKIVINLAPADIKKTGSAFDLPIALGILGASEQINESEKLK
                     DFIIMGELSLDGSIQPIKGALPIAIQARKEGFKGLIVPKQNAREAGMVNNILVYGVEH
                     INEVVNFFKDEQTLQPTIVNTREDFFNAQFEFEVDFNDVKGQENIKR"
     gene            730..1611
                     /locus_tag="PLU36_02200"
     CDS             730..1611
                     /locus_tag="PLU36_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HMZ45592.1"
                     /translation="MILLDGKLASASVKENLKKEVDKIIEKNLRPPHLAAILIGNNGA
                     SETYVASKIKSCKEVGFTSTLIRYEESVLEKVLLDKILELNNDPTIDGILVQLPLPKH
                     IVETKVIETINPKKDVDGFHPINAGKLVQGLETLIPATPYGVMLMLEHFNVDTKGKHA
                     VIIGRSNIVGRPMSILLSSNLLHGNCTVTLCHSHTPDLIEICKQADILVAALGKPESI
                     TAKMVKPGAVIIDVGITRIDDNTKKNGYRLKGDVKFDEVAAIASAITPVPGGVGLMTI
                     AGLLKNTLKAYQLNTKN"
     gene            1641..2282
                     /locus_tag="PLU36_02205"
     CDS             1641..2282
                     /locus_tag="PLU36_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HMZ45593.1"
                     /translation="MRKTSMLYPVMESFYTLQGEGFYTGAAAYFIRLAGCDVGCVWCD
                     VKESWNANEHPKYSVNDIIEKVLIEVNYHSISNKTNLPIIVITGGEPLMHNLNELTKA
                     LQQNGFKTNIETSGSSALSGSWDWICLSPKKFKPPIKENIVLANELKIIVYNKQDFKW
                     AESYAKQVNSHCKLYLQPEWSKRELITPLIVEYIKLNPQWQLSLQTHKYINVP"
     gene            2562..4475
                     /locus_tag="PLU36_02210"
     CDS             2562..4475
                     /locus_tag="PLU36_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ45594.1"
                     /translation="MKNIVLLFACIFCINVYAQTYNPEKINKKAVKIYMQAVEYLADG
                     MLPEALPYLNKAIATDTNYVEAILSLAGVYGQLKKHQLSVENYEWAKRKDTVYFRQYH
                     LPYSINLAGIGKFDEALEAINYFLSIPKISDKSIKAANYRKSCYQFALSYSTKHIDTS
                     YTFIPENLGDSINTETSEYYPSVTIDDSLLVFSRRGQGMREDFMQSKLDSNGYRKATF
                     INGDINLEPSKGALTISADGEWMIFAGNFGREKGFGSFDLYISFYTPEGWSDPENLGP
                     NINTEYWESSPSLSPDKRVLYFSSNRYGGVGGKDLYFSERMANGKFGPAVNMGKGINS
                     ESDEHSLFIHVDNQTLYYSSNGLPGYGGSDLYVLRKQADDTWSEPENLGYPVNTIDDE
                     GYFAVSADGLTAYFASDRADSRGGLDLYKFKLRNNIRPAKTLYVKGKVYDLHTNKGLP
                     CTIELIDNETGLALMKIQTDELGEYFITLPIGKNYTFSVNRKGYLFYNELYEMKNMVA
                     DSVYKKDIALQPVALHTSIAFKNIQFEHNSYKLLPVSLIELDKLFQLLKDNPTLQVEI
                     GGHTDNSGNTADNLKLSTNRAKAVVDYLVSKGIAINRLSYKGFGESKPIADNATAEGR
                     AINRRTTFTFTQM"
     gene            4499..5599
                     /gene="dprA"
                     /locus_tag="PLU36_02215"
     CDS             4499..5599
                     /gene="dprA"
                     /locus_tag="PLU36_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HMZ45595.1"
                     /translation="MNNDLLYQIALTLVPNIGAVQAKLLIENFDSAEAIFKAKIKDLS
                     RVEGIGEIRAKAIKNFTEFKLAEEEILFTEKYKIECLCLKNEQYPKRLLHCYDAPTIL
                     YYKGNANLNNSKIISIIGTRTNTDYGKQITQEIIEHLQPFHVLIVSGLAFGIDAIAHK
                     AALENNLATVGVLAHGLDSIYPTQHKKLAKEMLVNGGLLTECRKNIKADKFNFPQRNR
                     IVAGMADATIVVETAIKGGSMITAEMAYNYNKDVFAVPGKIYDNKSSGCLSLIQQNKA
                     ILLAKPQQIADELGWQTKTIKPPKQKELFVQLSSDEKIIINLLQLKETVAIDELFIQT
                     GLSSSAVAAALLNLELQNIVISLPGKIYKLIN"
     gene            5734..7755
                     /locus_tag="PLU36_02220"
     CDS             5734..7755
                     /locus_tag="PLU36_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMZ45596.1"
                     /translation="MAIAALLLVNSCNYSHKPEPINFKANEELENKFITFHLTDSVFL
                     NYTLDSLKKIYPQSHNTKVLYYYLLYLKEHYTNNYYENYTNAEALLNYLENNKLTTVY
                     SKYYTDALFAKGEYFFAARKYEEALKEYYKAKLILDAQTDTCNYSKYYNKLWNFYYSK
                     KDYRKALDYLNISIDREEKCKNNTAYFLYFHRIQSTLNTKALCYEKLGAYDSAIIFYQ
                     QGIKFIQNQESIHEINNQKRELALGIYYGNLGGAFAYNKQPDSAIYYLNKSIHINSKP
                     GYDSIDLQTAYLKLVDVYLSKKQYAKAKDILDFSDRLINSDSHPTIGYIRSKQRLLYY
                     NYYYQSGQLEKAIKAFESFFHVQDSVNQIAYDLNNFSIGKEVDKIKSEYLLKNFEKKN
                     EIKTIYIIVFSIVMALLFVIIILILRYLKQIKITANQTTLHNLQLQSTLQQFEQSNKN
                     FVRVMKVMAHDLRSPLAGITGITDILLNDRTIDENEKREMLTLIQSSSNNSLSMITEL
                     LSSSSNLEQKELVKEEINIIELLKQCTELLKFKANEKNISLHFHHSIESLNINADKEK
                     LWRVFSNLIMNAIKFSHSGKSVYIHVEKYLNEIVVNVQDEGIGIPEKYYSKVFDMFTE
                     AKRKGTSGEQPFGIGLHICKQIVEAHNGKIWFISNEPIGTSFYVSLPIN"
     gene            7801..8409
                     /gene="gldD"
                     /locus_tag="PLU36_02225"
     CDS             7801..8409
                     /gene="gldD"
                     /locus_tag="PLU36_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HMZ45597.1"
                     /translation="MKNKFSFLNFSACFLLIITTLLACNSVYTPKPKGYFKINFPEKK
                     YTTFQMKDYPYSFEYPVYAKVVKDSLFFGTKTENPWWINIDFPQFNGRIYVSYKLISN
                     NNFNDLVEDAFKLTNKHTQKATSIDDSLIITPNNVHGIYFKVGGDVATANQFFLTDSV
                     THFLRGALYFDATPNEDSLKIVNNFLLEDVKHLINTLRFTKK"
     gene            8500..9714
                     /locus_tag="PLU36_02230"
     CDS             8500..9714
                     /locus_tag="PLU36_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HMZ45598.1"
                     /translation="MAASKPVISTSFSYETLEETLDIKPDGAKLHIGIPKEIAFQENR
                     VALSPDAVGVLVSNGHTVMVETKAGEGCNYADKEYGEAGATITYDRTEVFKAPVLIKT
                     APPVQEDLPLIQNGQTIISPIHYSALNKATVKKLMEKKVTAISFDNLKDDSGTFPIVR
                     SMSEIAGGAVMLIAGQYLSSFNKGKGVLLGSISGIPPTKVVIIGAGIVGEVATRNALA
                     LGASVKVFDNNVYKLKQLQNNLGQRIWTSVLEPKILAKQLKTCEVAVGALSNAYGHSP
                     VVVTEEMVANMRKGSIIIDVAIDRGGCFETSQLTSHENPTFLKYDVIHYCVPNIPSGF
                     ARTASQAISNVIMHLILQISNEGGLDEIIWHNFNIREGIYLFKGALTDFYISQKFDLK
                     YTDLNLLIASRR"
     gene            complement(9735..10715)
                     /locus_tag="PLU36_02235"
     CDS             complement(9735..10715)
                     /locus_tag="PLU36_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HMZ45599.1"
                     /translation="MKFLVIRFSSIGDIVLTTPVVRCLKQQVTNSEIHFLTKASFSTI
                     VENNSYIDKVVLLNNNWDETILQLKQENYDYIIDLHHNLRTLRIKKALKIKSFSYNKL
                     NIQKWLLTAFKINTLPPIHIVDRYIETVKKIGVINDGKGLDYFIPEKDCIKQNDIPAQ
                     HQFGYIGIVIGAALNTKKMPVNKLIDLCSKIDFPIILLGGKEDKENGDTIAAIDTIKI
                     YNACGKFNLNESADLVRKAKLIITHDTGLMHIAAAFKKPILSVWGNTIPSFGMSPYFG
                     NNQITNLHYEVKLGCRPCSKIGFNKCPKKHFKCMEQQDVSQIAKDALKLV"
     gene            10838..11719
                     /locus_tag="PLU36_02240"
     CDS             10838..11719
                     /locus_tag="PLU36_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HMZ45600.1"
                     /translation="MFYFYYALFYLLSLLPWRVMYFLSDCFYLLAYYVIRYRRNVVAN
                     NLQIAFPDKTERERIKISKAFYHNLIDSFVESIKLLTISRKEFNKRCITNFKVVNDLY
                     STGQSVQLLSGHFFNSEFLNLGCAANLKYPFLGVYMGLSNKNLEKVMVIMRKKFGTIL
                     FPASEFRTEFPKHSKSVYSIGLIADQNPPIPTNAVWFPFFGRMTPFHTGPEKGAIRMN
                     TAIAFIEMTKVKRGYYKLEAKLVTTTPKEFLEGTITKQLVAFIEETVKQHPDNYLWSH
                     RRFKYEFDEKKYGHLVI"
     gene            complement(11790..13421)
                     /locus_tag="PLU36_02245"
     CDS             complement(11790..13421)
                     /locus_tag="PLU36_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HMZ45601.1"
                     /translation="MWKKHPKALPFLFFSEMWERFGYYLMIGIFTLYLKDVKAGFAMT
                     EAESADLYGTFIALVFLTPFIGGLIADRYWGYRKSIIIGGLLMGAGYCLMSIHNIAAL
                     YASITLVIVGNGFFKPNISTLLGNVYTTPQHNHQKDEGYNIFYMGINIGAFICNFFGA
                     VLYNILGWGAAFFAAGIGMFIGVLIFVIGTKHYAAFDIKKGVKEGDMSMGKITVLILL
                     PSVLAGVLGWLLPGNIFGSDSTDAFIFACVPVIFFYASLYFKADENEKTPIGVLLTIF
                     AVVIMFWAVFKQNGTALNTWADRYTDRSVSGTTEKVFKAVQFTQTLKYQKDSVPLYDS
                     MFRLQKQNGVVIKEFNYPVYFRNDMSSKMQTEGGELSIWATNLSQSINPAWVIILTPL
                     IVAFFTFLRGRKKEPSTPTKIAFGLFVSALSVLVMIAAVKAGNNGAEKVSVWWLIGSY
                     GVVTIGELLLSPMGLSLVSKLSPVRITSLMMGGWFLSTSIGNKLSGVLASMWDKYDDK
                     ANFFWLNFFLLLFSASIMFLMLKRLNKVLKVRGVK"
     gene            complement(13493..14989)
                     /locus_tag="PLU36_02250"
     CDS             complement(13493..14989)
                     /locus_tag="PLU36_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HMZ45602.1"
                     /translation="MNQTAAPTSGHPKGLYLLFFTEMWERFSYYGMRAIFVLYMTKML
                     LMPDDTASNIYGSYTGLVYLTPLLGGYLSDRFLGNRRSIEIGGVLMALGQFLMFFSAS
                     ATGTMAITFMWAGLTALIIGNGFFKPNISTMVGQLYPAGDRRVDSAFTIFYMGINLGA
                     FFSPLVCGTLAEKIDFKWGFFAAGIGMVIGLLLFMALKNKYLNTEDGKPIGKSVKPLD
                     TKNIFYLVGSIALIFFLLNFKTLFNNNWDVIGWVIYGSMIAIPVIILSDKTITPVERS
                     RILVIFILAFFVIFFWGAFEQAGASLTIFADRQTDRHIFGWEMPASYFQSINPLAIIA
                     LAPVFSALWLTLGNKNAEPSTPKKMALGLVLVAVGYLLIAYAVKGVDASTKVSMFWLF
                     SLYIVHTMGELCLSPIGLSMVAKLSPLRFSSLLMGTWFLANAAANKFAGTLSELIPSE
                     TKPTTSFIGIQITNLYEFFTVFIVMCGLAAGILFMLSGKLQKMMHGIK"
     gene            15150..>15607
                     /locus_tag="PLU36_02255"
     CDS             15150..>15607
                     /locus_tag="PLU36_02255"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613883.1"
                     /note="catalyzes the formation of
                     N(1)-(5-phospho-D-ribosyl)glycinamide from
                     5-phospho-D-ribosylamine and glycine in purine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HMZ45603.1"
                     /translation="MNILLLGSGGRENALAWKMKQSHHCNNLFIAPGNAGTNLIGINV
                     SIDVNDFESQKQFCIEKNIDIVVVGPEEPLVNGVFDYYKKDELLQHIIVVGPSAKAAQ
                     LEGSKAFSKQFMQTYKIPTATYAEFSVSEIEEAKKYIQQHSLPIVVKADG"
BASE COUNT         5832 a         2302 c         2406 g         5067 t
ORIGIN      
        1 cccttttaat gttttcttgc ccttttacat cgttaaaatc aacttcaaat tcaaactgtg
       61 cattaaaaaa atcttctctg gtatttacaa ttgttggttg taaagtttgc tcatctttaa
      121 aaaaattaac tacttcatta atatgttcta caccatacac caaaatatta ttcaccattc
      181 ctgcctctct tgcattttgc ttgggaacaa ttagaccttt aaagccttct tttcttgctt
      241 gtatggcaat gggtaaagca cctttaatag gttgtataga gccatctaaa ctcaattcgc
      301 ccattataat aaaatctttt agtttttcag actcattaat ttgttcactt gcacctaaaa
      361 ttcctaaggc aattggtaaa tcaaaagcac tgcctgtttt ttttatatca gcaggagcta
      421 agtttataac aattttcgtt ctaggaaaac taagctgatt agttttaatg gcactttcaa
      481 ttctttgcaa actttctttc actgcattat ctggtaaacc taccatgtag tattttaaat
      541 caccaccact aacattcact tctatagtaa tagaaattgc ttcaacacca tatacggcac
      601 tgccaaatgt ttttacaagc atggtaggtt tttaaaaaaa taatagttta ttacttgtaa
      661 tattaagaat aaaatactta taaaccattt ttttactgct gcatagtttg tagatattaa
      721 ctttgcagta tgatattgtt agatggaaaa ttggcttcgg ctagtgttaa agaaaatctt
      781 aaaaaagaag ttgataagat aattgaaaag aatcttagac ctcctcattt agcagcaatt
      841 ttaataggca ataatggtgc aagcgaaact tatgtagcca gtaaaataaa aagttgtaaa
      901 gaggttggtt tcacttctac tttaattaga tatgaagaat ctgtattgga aaaggtatta
      961 ctagataaaa ttctagaatt aaacaatgat cctactatag atggcatttt agttcagtta
     1021 cccttaccta aacatattgt tgaaactaaa gttatagaaa ctataaaccc taaaaaagat
     1081 gtagatggat ttcacccaat aaatgcagga aaacttgtac aagggttaga aactttaata
     1141 cctgctacac cttatggagt aatgctaatg ctagaacatt ttaatgtaga tactaagggt
     1201 aaacatgctg ttattattgg tagaagtaat attgtgggca ggccaatgag cattttactt
     1261 agtagtaatt tattgcatgg aaattgcaca gttaccttat gtcatagcca tactccagat
     1321 ttaatagaaa tttgtaagca agccgatatt ttggttgcag cattaggtaa accagaatct
     1381 attactgcca aaatggtaaa accaggtgct gtaattattg atgttggaat tactagaatt
     1441 gatgataaca ccaaaaagaa tggctatcgc ctaaaaggtg atgtaaaatt tgatgaagta
     1501 gctgctattg caagtgctat tactcctgta ccaggtggtg ttggtttaat gaccattgca
     1561 gggttattaa aaaatacttt aaaagcctat caactaaata ctaaaaatta agtaaaagaa
     1621 aaatcgttta atcattttta atgcgtaaaa cttctatgtt atatcctgta atggagagct
     1681 tttatacctt acaaggcgaa gggttttata caggggctgc agcatatttc attaggcttg
     1741 caggttgcga tgttggttgt gtttggtgcg atgtaaaaga aagttggaac gccaatgagc
     1801 acccaaaata tagcgttaat gatattattg aaaaagtttt aattgaagta aattatcatt
     1861 caattagtaa caaaacaaat ttaccaataa ttgttattac aggtggcgag cctttaatgc
     1921 ataatttgaa tgagttaaca aaagcactac aacaaaatgg gtttaaaaca aatattgaaa
     1981 ctagtggatc atctgcatta agtggtagtt gggactggat ttgcctttcg ccaaaaaaat
     2041 ttaaacctcc aattaaagaa aacattgtac tagcaaatga gttaaaaatt atagtgtaca
     2101 acaaacaaga ttttaagtgg gcagaaagtt atgccaaaca agtaaatagc cattgtaaac
     2161 tttatttaca accagagtgg agtaaaagag aattgataac cccattaatt gttgagtata
     2221 ttaaattaaa ccctcaatgg caattaagct tacaaacaca taagtatata aatgttcctt
     2281 aaacgaactt gttagtttaa tttaatgtaa ctcttttcaa tataagtatg gtttgttgta
     2341 acattaactt tcaaattgct ttaaagcaac aataaaaaat catatttata attataactt
     2401 tagttttttt attagcagtt tctatgttaa aaataagttt tcattagtgg tttgcaaata
     2461 catttttagt tttgaaaata tttacctttt ttgctattta acaaataaac aaatctccta
     2521 attatccata tttaaaaaat tggtattacc tttcgcaaac aatgaaaaat attgttttac
     2581 tttttgcatg tatattttgt attaacgtat atgcacaaac ttataatcct gaaaaaataa
     2641 ataaaaaagc tgtaaaaata tatatgcagg ctgtggagta tttggcagat ggtatgttgc
     2701 cagaagcctt accctatttg aataaagcca ttgcaacaga cactaattat gtagaagcta
     2761 ttctatcttt agcaggtgtt tatggccagt taaaaaagca tcaactatct gtagagaatt
     2821 atgaatgggc taaacgaaaa gatactgttt acttcaggca ataccatttg ccctattcta
     2881 ttaatcttgc aggaattggt aagtttgatg aagctcttga agcaataaat tattttttaa
     2941 gtattccaaa aattagcgac aaaagtatta aagcagcaaa ctacagaaaa agttgttatc
     3001 aatttgcact ttcctattct accaaacata ttgataccag ctatacattt attccagaaa
     3061 atttaggaga tagtataaat accgaaacaa gtgaatatta tccaagtgtt acaattgatg
     3121 atagcttatt agttttctcc agaagagggc aaggtatgag agaagatttt atgcaaagta
     3181 aattagatag taatggctat cgcaaagcaa cttttattaa cggagatatt aatttagaac
     3241 cctccaaagg tgctttaact atatcggcag atggcgagtg gatgattttt gcaggaaatt
     3301 ttggaagaga aaaaggcttt ggaagttttg atttgtacat ttctttttac acaccagagg
     3361 gatggagtga tccggaaaac ttagggccaa atattaatac agagtattgg gagagcagcc
     3421 caagccttag cccagataaa agagtacttt attttagcag taataggtac ggtggcgtag
     3481 gaggcaaaga tttatacttt agcgaaagaa tggcaaacgg caaatttgga cctgcagtta
     3541 acatgggaaa aggaattaac tccgaatcag atgaacattc tcttttcata catgtagata
     3601 accaaacatt atattattct agcaatggtt tgccaggcta tggtggtagt gatttatatg
     3661 ttttaagaaa acaggcagat gacacttgga gcgaacctga aaacttaggc taccctgtta
     3721 ataccattga tgatgaagga tattttgctg taagtgccga tggtttaact gcatactttg
     3781 caagtgatag agcagattcg agaggcggat tagatttata taaattcaaa ttaagaaata
     3841 atataagacc tgcaaaaact ttatacgtaa aaggcaaagt gtacgatttg catacaaaca
     3901 aaggattgcc ctgtaccata gaattaatag ataatgaaac aggtttggca ttaatgaaga
     3961 ttcaaacaga tgaattggga gaatatttta taacattacc aattggtaaa aactatacct
     4021 tctctgtaaa cagaaaagga tatttatttt ataatgaatt atatgaaatg aaaaatatgg
     4081 ttgcagatag tgtatataaa aaagatattg ccctacaacc agttgctcta cataccagta
     4141 ttgcatttaa aaatattcag tttgaacata actcttataa attattacca gtaagcttga
     4201 tagaattaga taagctattt caacttttaa aagataaccc aacactacaa gtagaaattg
     4261 gaggacatac tgataatagc ggcaacactg cagataattt aaagttaagt accaacagag
     4321 ctaaagctgt agtagattat ttagtgagta aaggaattgc tataaacaga ctatcttata
     4381 aaggttttgg cgaatcaaaa cctattgcag ataatgctac agcagaggga agagcaataa
     4441 acagaagaac tacttttaca tttacacaaa tgtaaaaaaa tgtaaccatc attattcaat
     4501 gaataacgat ttattatacc aaattgcatt aacacttgta cccaatattg gagcagttca
     4561 agcaaagtta ttgatagaaa attttgatag tgctgaagca atttttaaag caaaaattaa
     4621 agatttaagt agggtagaag gtattggaga aataagagca aaagctatca aaaattttac
     4681 cgaatttaaa cttgcagaag aagagatatt gtttacagaa aaatataaaa ttgaatgttt
     4741 atgtttaaaa aatgagcagt atcctaaaag gttattacat tgttacgatg ctccaacaat
     4801 tttatattat aaagggaatg ccaatttaaa caactccaaa ataattagta taattggtac
     4861 aagaacaaat acagattatg gtaaacaaat aacacaagaa attatagaac atttacaacc
     4921 ttttcatgta cttattgtaa gtggtttagc atttggaata gatgccattg cacataaagc
     4981 tgcattagaa aataatttag caactgttgg tgtattagct catggcttag atagtattta
     5041 cccaacacaa cacaaaaaac ttgcaaaaga aatgttggta aatggtggct tattaacgga
     5101 atgtagaaaa aatataaaag ccgataaatt taattttcct caaagaaata gaattgtagc
     5161 aggcatggca gatgccacaa tagtagttga aacagctatt aaaggtggta gtatgataac
     5221 agcagaaatg gcctataatt ataataaaga tgtgtttgct gtaccaggta aaatttatga
     5281 taataaaagt agtggatgcc ttagtttaat tcaacaaaac aaagctattt tattagccaa
     5341 gccacaacaa attgcagatg agttgggttg gcaaaccaaa accataaaac ctccaaaaca
     5401 aaaagaatta tttgttcaat tatcatcaga tgaaaaaatt ataattaatc ttctacaatt
     5461 aaaagaaaca gtagctatag atgaattatt tattcaaact gggctaagta gtagtgctgt
     5521 agctgcagca ttactaaatt tagagttaca aaacatagta attagtttac ctggtaaaat
     5581 ttacaagtta attaactgat tatataattt taatttttca tattaattat ttgcttacat
     5641 tgcaacggct aaatttgaaa tttcactctt taagacatga tgctatttgg taagtgtaag
     5701 aactataaat atccaatttt atttttttat ctgttggcaa tagcagcact attgctagtt
     5761 aatagttgta attactccca caaaccagag ccaatcaatt ttaaagcaaa cgaagagcta
     5821 gagaataaat ttattacttt tcacttaacc gattctgttt ttttaaatta tactttagat
     5881 agcttaaaga aaatctaccc acaatcgcac aacactaaag ttttatatta ttatttactt
     5941 tatttaaaag aacattatac caataactat tatgagaatt acactaatgc agaggcatta
     6001 cttaattatt tagaaaataa taaactaact acagtttact ctaagtacta tacagatgct
     6061 ttatttgcta aaggagaata tttttttgct gcaagaaaat acgaagaggc attaaaagaa
     6121 tattataaag ctaaattaat tttagatgcc caaaccgata cttgtaatta tagcaagtat
     6181 tacaataagt tgtggaattt ttattactcc aaaaaagatt acagaaaagc cctagattac
     6241 ttaaatatta gtatagatag agaagaaaaa tgtaaaaata acaccgctta ttttttatat
     6301 tttcatcgaa ttcaatcaac gttaaatact aaagctttat gttatgaaaa attaggagct
     6361 tatgatagtg ccattatatt ttaccaacaa ggcattaaat ttattcaaaa tcaagaatct
     6421 atacatgaaa taaacaatca gaaaagagaa ttagcattag gaatttatta tggaaattta
     6481 ggtggagcat ttgcttataa taagcaacca gactctgcta tttattattt aaataaatcc
     6541 atccatatta attcaaaacc cggttatgat tctattgatt tacaaactgc ctatttaaaa
     6601 cttgtggatg tttatttaag taaaaagcaa tatgcaaaag caaaagatat tttagatttt
     6661 tctgataggt tgataaattc agattcacat cctaccattg gttacattag aagtaagcaa
     6721 cgattattat attataatta ttattaccaa tcaggccaat tagaaaaggc aattaaagca
     6781 tttgaaagtt tttttcatgt gcaagactca gtaaatcaaa ttgcttatga tttaaataat
     6841 tttagtatag gcaaagaagt agataaaatt aaaagcgaat atttattaaa aaatttcgaa
     6901 aaaaagaatg aaattaaaac catttacatt attgtatttt caatagtaat ggcattatta
     6961 tttgttatta taatattaat tttaagatat ttaaagcaaa taaaaattac tgcaaaccaa
     7021 accacacttc ataatttaca gttacaaagt accttacaac aatttgaaca aagtaataaa
     7081 aattttgtac gtgtaatgaa ggtaatggcc catgacttaa gaagcccctt agcaggcata
     7141 acaggtatta cagatatttt attgaatgat cgtactattg atgaaaatga aaaaagggaa
     7201 atgctaactt taatacaatc gtcaagcaat aattcattaa gtatgattac tgaattgctt
     7261 agtagcagct ctaacttaga acaaaaagaa ttagtaaaag aagaaataaa tattattgaa
     7321 ttattaaaac aatgtaccga attattaaag tttaaagcaa atgaaaaaaa cataagtctt
     7381 cattttcatc attccataga aagtttaaat ataaatgcag ataaagaaaa attatggagg
     7441 gttttctcta atttaataat gaatgcaata aaatttagcc attcaggaaa atctgtttat
     7501 atacatgtag aaaaatattt gaacgaaatt gtagtaaatg ttcaagatga aggtatagga
     7561 atacctgaga aatattatag taaagtgttt gatatgttta cagaagcaaa acgtaaaggc
     7621 acatctggtg aacaaccttt tggaattggc ttacatatct gtaaacaaat agtagaagca
     7681 cacaatggta aaatatggtt tatttctaat gaacctattg gtacatcatt ttatgtttcc
     7741 ctgcctatta attaatagta ttactttgca ttctttccac acaaaaaata atttatgggt
     7801 atgaaaaata aattttcatt tttaaatttc agtgcttgtt ttctattaat aataactaca
     7861 ctattggctt gtaatagtgt ttatactcca aaaccaaaag gctattttaa aataaatttt
     7921 cccgaaaaaa aatatactac ttttcaaatg aaagactatc cttatagttt tgaatacccg
     7981 gtttatgcaa aagtggttaa agattcttta ttttttggaa ccaaaacaga aaacccttgg
     8041 tggattaata tagattttcc tcagtttaat ggaagaattt atgtaagcta taaactaatc
     8101 tccaataata attttaatga tttagtagag gatgctttta aattaaccaa caagcataca
     8161 caaaaagcaa catctataga cgattcatta attattacac ctaacaatgt acatggaatt
     8221 tattttaaag taggcggaga tgttgcaact gcaaatcaat tttttttaac ggatagtgta
     8281 actcattttt taagaggggc attatatttt gatgccacac ctaatgaaga ctcattgaag
     8341 attgtaaaca actttttgtt agaagatgta aaacacctta ttaatacttt aaggtttaca
     8401 aaaaaataag attaccgctt gtttgtgcag cataaaaaaa ctatttcatt taaactaaat
     8461 tactataact aactttaggc aataattaat aaatcagtta tggctgcttc taaacctgtt
     8521 atatccactt cttttagtta cgaaacacta gaagaaacct tggacataaa accagatggt
     8581 gcaaaactac acataggcat acccaaagaa attgcttttc aagaaaacag ggtagctctc
     8641 tcgcctgatg ctgtaggcgt gttggtaagt aatggccata cggttatggt agaaactaaa
     8701 gcaggagaag gatgtaacta tgcagataaa gaatatggcg aagcaggggc aaccattact
     8761 tacgacagaa ctgaagtttt taaagcaccg gttttaataa aaactgcacc acccgtacaa
     8821 gaagatttac ctcttattca aaatgggcaa actattatat ctcctattca ctattctgct
     8881 ttaaataaag caacagttaa aaaattaatg gagaaaaaag ttactgcaat tagctttgat
     8941 aatttaaaag atgatagtgg cacttttcca attgtaagaa gtatgagtga aattgcaggt
     9001 ggagcagtaa tgctaattgc tgggcaatat ttaagtagct ttaataaagg taaaggagtt
     9061 ttgttaggta gtattagcgg tattccacct actaaagttg taataattgg agctggaatt
     9121 gtaggtgaag tagcaactag aaatgccttg gctttaggag caagtgttaa agtttttgat
     9181 aataatgttt acaagctaaa acaactacaa aataatttag gacaaagaat ttggacaagt
     9241 gttcttgaac ctaaaatttt agcaaaacaa ttaaaaacct gcgaagtggc agtaggagct
     9301 ttaagcaatg cttatggcca ttcgccagtt gtagttaccg aagaaatggt tgccaatatg
     9361 cgtaagggaa gcataattat agatgttgct attgatagag gtgggtgttt tgaaacaagc
     9421 caactaacca gccacgaaaa cccaaccttt ttaaaatacg atgtaattca ttattgtgta
     9481 ccaaacatac caagtggttt tgcaagaact gcaagccaag caattagtaa tgtaattatg
     9541 caccttattt tacaaataag taacgaagga ggtttagacg aaattatttg gcacaacttt
     9601 aatataagag aaggtattta tttgtttaaa ggagccttaa cagatttcta catcagtcaa
     9661 aaatttgatt taaaatacac agatttgaat ttattaattg ccagcagaag ataacaattc
     9721 aatttatata ctttctacac taactttaat gcatcttttg caatttgaga cacatcttgt
     9781 tgttccatac atttaaaatg ttttttagga catttgttaa aaccaatttt actacatggg
     9841 cggcatccaa gttttacttc ataatgtaaa ttggtaattt ggttgtttcc aaaataaggc
     9901 gacattccaa aactcggaat tgtattaccc caaacagaaa gaatgggttt tttaaatgct
     9961 gctgctatat gcattaatcc agtatcgtgt gttataatta gtttagcttt tcttactaaa
    10021 tcagcacttt cattaaggtt aaacttaccg caagcattgt atatttttat tgtatctatt
    10081 gctgcaattg tatctccatt ttctttgtct tctttacctc ctaaaagaat aataggaaaa
    10141 tctattttac tgcataaatc tatcagtttg tttacaggca tttttttggt atttaatgct
    10201 gcaccaatta caatacctat atatccaaac tggtgttgtg caggtatatc attttgttta
    10261 atgcaatctt tttcaggtat aaaataatct aatcctttac catcgttaat tactcctatt
    10321 ttttttacgg tttctatata cctatccaca atatgtatgg gtggtagtgt attaatttta
    10381 aaagcggtga gcaaccattt ttgaatattc agtttattat aggagaacga ttttattttt
    10441 aaagctttct ttattcttag tgttcttaaa ttatgatgta agtcaataat ataatcataa
    10501 ttttcctgtt ttagttgcag tatagtctca tcccaattat tgtttaataa aacaacttta
    10561 tctatgtaag aattattttc aacaatagta gaaaatgagg cctttgttaa aaaatgtatc
    10621 tctgaatttg ttacttgttg ttttaagcat cttacaactg gtgttgtaag cacaatatct
    10681 ccaatagagg agaaacgtat tacaagaaat ttcattcaac aaagttgcta ccaaagcctt
    10741 taattttttt atttgttgat aaaaaattaa aaagctacgg attattagtt ataatttttt
    10801 atgcagctat tgtttacttt tgttatttta caacaacatg ttctatttct actacgcatt
    10861 attttaccta ttatcattac taccttggag ggtaatgtat tttttgagcg attgttttta
    10921 cttattagca tattatgtaa ttaggtatag aagaaatgta gttgccaata atttacaaat
    10981 tgcatttcct gataaaactg aaagagaaag aattaaaatt tcaaaagctt tttatcataa
    11041 tttaatagat tcttttgtgg aaagtataaa gctcttaact atttctagaa aagagtttaa
    11101 taaacgatgt attacaaatt ttaaagtagt aaatgatttg tattcaacag ggcaaagtgt
    11161 tcaattatta tctggtcatt tttttaattc cgaatttttg aatttaggtt gtgcagccaa
    11221 tcttaaatat cctttcttag gtgtttatat ggggctttct aacaaaaatt tagaaaaggt
    11281 aatggtaata atgagaaaga aatttggtac aattcttttt cctgcatccg aatttagaac
    11341 agaatttcct aagcatagca aaagcgttta ttctattggg cttattgcag atcaaaatcc
    11401 accaatacca accaatgctg tttggtttcc tttttttggt agaatgacac catttcatac
    11461 agggccagaa aaaggagcaa tacgtatgaa tacagctata gcttttatag agatgacaaa
    11521 agtaaaaaga ggctactaca aattagaagc taaattggtt acaacaacac caaaagaatt
    11581 tctagaaggc actattacaa aacaactagt tgcatttata gaagaaacag tgaaacaaca
    11641 tccagataac tatttatgga gccatagaag atttaaatat gaatttgatg aaaaaaagta
    11701 tggtcattta gtaatttaaa taatttcttt agttaataaa aaaatgccag cacaaaggct
    11761 ggcaaacaat aaaattttga ttaaaatatt tatttaactc ctctaacttt taaaacttta
    11821 ttaagcctct taagcattaa aaacataatg gatgcagaga ataataacaa aaagaaattt
    11881 agccagaaaa agtttgcttt atcatcgtac ttatcccaca tacttgctaa aacaccagat
    11941 agtttattac ctattgaagt agataaaaac catccaccca tcattaaaga agtaatacgc
    12001 acagggctta gtttagatac taaagataaa cccattgggc ttagtaaaag ttcaccaatg
    12061 gtaacaactc cataactacc aattaaccac caaacactta ctttttctgc cccattatta
    12121 ccagctttta ctgctgcaat cataactaaa acagaaagtg cagaaacaaa taatccaaat
    12181 gcaatcttag taggtgtact aggttctttt tttctacctc ttaaaaatgt aaaaaatgct
    12241 actattaacg gtgtaagaat aattacccaa gcagggttaa tactttggct taaattagta
    12301 gcccaaatgc ttagttcgcc accttcagtt tgcattttac tactcatgtc atttctaaaa
    12361 taaactgggt aattaaactc ttttattact actccatttt gtttttgaag cctaaacata
    12421 gagtcatata atggcacaga atctttttgg tactttaagg tttgtgtaaa ttgtactgct
    12481 ttaaaaactt tttctgtggt gcccgataca gaacgatctg tgtatctatc tgcccaagtg
    12541 tttaatgcgg tgccattttg tttaaataca gcccaaaaca taattacaac agcaaaaatg
    12601 gtaagcagta cgcctatagg tgttttttca ttttcatctg ctttaaaata caaagaggca
    12661 taaaaaaata taactggtac acaagcaaaa ataaaagcat cggtagagtc gctaccaaaa
    12721 atattacctg gcaaaagcca tcctaaaaca ccagcaagta cagatggtaa aagaataaga
    12781 actgtaattt ttcccatact catatctcct tctttaaccc cttttttaat atcaaatgct
    12841 gcataatgtt ttgttccaat tacaaaaatt aaaacgccaa taaacatacc aatacctgca
    12901 gcaaaaaatg ctgcccccca acctaaaata ttgtataata cggcaccaaa aaaattacaa
    12961 ataaatgcac caatattaat acccatataa aaaatattat acccttcatc tttttgatga
    13021 ttgtgttgtg gtgtagtgta cacatttcct aagagggttg aaatattagg tttaaaaaaa
    13081 ccatttccaa caattactaa agtaatacta gcatataatg cagctatatt gtgtatgctc
    13141 attaagcaat agccagcacc cattaaaagc ccaccaatta tgatagattt tctatacccc
    13201 caatatctat cggcaattaa tcctccaata aaaggagtaa gaaaaaccaa tgctataaat
    13261 gttccatata agtcagcact ctcagcctcg gtcattgcaa agcctgcttt aacatcttta
    13321 aggtataaag taaaaatacc tatcattaaa taataaccaa accgttccca catttcagaa
    13381 aaaaataaaa agggtaaagc tttagggtgt tttttccaca tgattaaaaa gtttaaataa
    13441 taaaaaagcg gtgaagaaat taaatcaccg ctttaaaaat aagaaagttc aattatttaa
    13501 tgccatgcat cattttttgt agtttaccac ttaacatgaa taaaatgcca gcagctaaac
    13561 cgcacataac aataaatact gtaaagaatt cgtataaatt agttatttga ataccaataa
    13621 atgaagtagt aggttttgtt tcgctaggaa ttaattcact taatgtgcca gcaaatttat
    13681 ttgcagctgc attggctaaa aaccaagttc ccattagtaa agaggaaaat cttaatggcg
    13741 ataattttgc aaccatggat aatcctatag gtgataaaca tagttctccc atagtatgca
    13801 caatgtataa actaaataac caaaacatgc ttactttggt ggatgcatcc acacctttaa
    13861 ctgcatatgc aattaataaa taacctacag caaccaatac taaacctagt gccatttttt
    13921 taggagtaga aggttctgca tttttatttc ctaaggttaa ccataaagca gagaatacag
    13981 gagcaagtgc tataatggct aatgggttga tagactgaaa ataacttgca ggcatttccc
    14041 aaccaaaaat atgtctatct gtttgtctgt ctgcaaaaat ggttaacgat gcacctgcct
    14101 gctcaaaagc accccaaaag aaaattacaa aaaatgccag aataaaaatt actaaaattc
    14161 tacttctttc taccggtgta atagttttat cagataatat aattacaggt atggcaatca
    14221 tagagccata tattacccag ccaattacat cccaattatt attaaaaaga gttttaaaat
    14281 ttaagagaaa aaatattaaa gcaatggagc caactaggta aaaaatattt ttagtatcta
    14341 atggttttac agattttcca attggttttc catcttcggt attaaggtat ttatttttta
    14401 aagccataaa taatagcaag ccaataacca ttcctatacc agctgcaaaa aatccccatt
    14461 taaaatctat tttttctgct aaagtaccac atactaaagg agagaagaaa gcacccaaat
    14521 taatacccat ataaaaaatt gtgaaagcac tatcaactct tctatctcct gctgggtaca
    14581 attggccaac cattgttgaa atattgggtt taaaaaaacc attcccaatt attagagctg
    14641 ttaagccagc ccacataaat gtaattgcca ttgttccggt tgccgaggca ctaaaaaaca
    14701 ttaaaaattg ccctaatgcc atcaatactc ctccaatttc aatacttctt ctattaccta
    14761 aaaaacggtc gcttaaatag ccacctaata aaggagttaa atacactaaa ccggtataac
    14821 taccataaat atttgaggca gtatcatcgg gcattaaaag cattttggtc atatataaca
    14881 caaaaattgc tctcatgcca taataactaa aacgttccca catttcagta aaaaataata
    14941 aataaagacc cttaggatgt cctgaagtag gtgcagcagt ttgattcata tacaattaat
    15001 tttcgttaga ggttattttg aaggcttgaa aataagtaga aaagttaata aacttcttaa
    15061 aaagttttaa cagttaaagt agaggaataa aaattcaatg ttttgtttaa aaaatcgcat
    15121 ttactttcgc cttaaataat tatgtaaata tgaatattct tttgttaggt agtggtggaa
    15181 gagaaaatgc tttggcatgg aagatgaagc aaagccatca ttgtaataat ttatttatag
    15241 caccaggtaa tgctggtacc aatttaatag gaataaatgt atctattgat gtaaatgatt
    15301 ttgaaagtca aaagcaattt tgcatagaaa aaaatataga tattgttgtt gtaggtccag
    15361 aagagccttt ggtaaatggt gtgtttgatt attacaagaa agatgagttg ctacaacata
    15421 taattgtagt tggtccatct gctaaagctg cacagttaga aggtagtaaa gcatttagta
    15481 aacaatttat gcaaacctat aaaattccta cagctactta tgcagagttt tctgttagtg
    15541 aaatagagga agcaaaaaaa tatatacaac aacatagttt acccattgtt gttaaggcag
    15601 atggttt
//
LOCUS       DAOQPK010000025        15367 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_1951_length_15367_cov_5.141001, whole genome shotgun sequence.
ACCESSION   DAOQPK010000025 DAOQPK010000000
VERSION     DAOQPK010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 15367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15367
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1951_length_15367_cov_5.141001"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="PLU36_02260"
     CDS             complement(<1..603)
                     /locus_tag="PLU36_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HMZ45604.1"
                     /translation="MFTTKNLSPKQLSAFTSFIVSLPASLVFFVIEKQVWIGFAAFVV
                     MFIGSYFLISFVLEWFIYRKIKLIYKFIFNTKASKKEETYYKYILPKKSIDEVKEDVE
                     KWADQRKAEIELLKQNEAFRKEFLQNISHEFKTPIFAIQGYIEALQAEGLKDNETNQK
                     FLNNAANNVERMVNLVNDLDEITKLERGEQPLNKSNFIIQD"
     gene            complement(706..1290)
                     /locus_tag="PLU36_02265"
     CDS             complement(706..1290)
                     /locus_tag="PLU36_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HMZ45605.1"
                     /translation="MIKNICKICVVFVFSFLFITKDLIAQEAAKVPEIDKSPMDISYF
                     PKDYPLMKMNGKSKDLPIARVIYSRPMKKGREIFGGIIQYKEIWRLGANEATEIEFFR
                     NVKIAGKTIAKGRYSLLCIPQPTKWTIIFNKDNYSWGSFNYDAKKDLLRVDCTLEKTE
                     EAIEALSIYFEPLKNGADLIIAWDNTKTKLAITW"
     gene            1350..1937
                     /locus_tag="PLU36_02270"
     CDS             1350..1937
                     /locus_tag="PLU36_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor"
                     /protein_id="HMZ45606.1"
                     /translation="MKKILRVIKIWNINNYYATFATEIETDERNEVVPFFFIPMSADS
                     NLNKIEIFINGLLQSEPNYFLVDLKVKPTNNIKVFIDGDEGITIEKCIYFNRKLYKLI
                     EEAGIYEEGNFSLEISSPGVGEPLKLKRQYQKNIGRNIEVIFIDESKKDGKLLDVMED
                     SILVENTTGKGKKAVTQQLVISFLNIKTTTVQIKF"
     gene            1982..3238
                     /gene="nusA"
                     /locus_tag="PLU36_02275"
     CDS             1982..3238
                     /gene="nusA"
                     /locus_tag="PLU36_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HMZ45607.1"
                     /translation="MTRINLIDAFQDFKDAENLDRPTLMKVVEDVFKTLLRKRYGADD
                     NFDVIVNAEKGDLEIIRRRMIVEDGEVLDPLAEIGYTEATKIDPDFEVGEDLYEEVNM
                     IDFGRRAILAAKQTLASRINDLKKNVLVKKYADRIGEIISAEVYQVWKKEVLLLDEDG
                     NELILPKSEQIPQDFFKKGENIRAVVEKVDLKNNAPVIILSRTSHKFLAKLLEIEVPE
                     IFDGLITIKKIVREPGERAKVAVESYDDRIDPVGACVGMKGSRIHGIVRELKNENIDV
                     INYTTNIQLLIQRSLTPAKISFMNLDNDKKQADVYLKADQVSLAIGKRGVNIKLACEL
                     TGYEIDVYREDEEEQEYEFDIDLDEFSNEIEQWIIDELKRVGCDTAQSVLKLSNIEIE
                     RRTDLEKETIEEIRSILQAEFDKEAE"
     gene            3326..6229
                     /gene="infB"
                     /locus_tag="PLU36_02280"
     CDS             3326..6229
                     /gene="infB"
                     /locus_tag="PLU36_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HMZ45608.1"
                     /translation="MSELKLPRLLAAAKEFNIGQETLIDFLAKKGFNKDDLKPTAKLS
                     EEMYYALQAEFQSDKAAKNKADQIEIPKSVAPDKKKKEEETVTKKVEKKAEEIKVEPS
                     PIKEEPLEEKKPDEVKKVEVEVEKKIEQKTTATEIVAPKVVDKIDLSAIDSSTRPKKT
                     AKAKTTEPTKEQPSSKKEETAKVEQQEPKVEPAITEETNSIVKPPDTASTIVENIEVE
                     KLTGPKILGKITLPVDSDTRPKPISKVEQHKRKRIVVDKKPGSNIQRVDFRKEGGGQT
                     QTGNNNRGGNQQQQNRFGGKPGGGGGHPQRGGGRNAGNNRGKQDNRRVADKEIDAKEI
                     QDKIKETQAKLAGSGGRGKSLKAKYRREKRQEAAEAAMEGSGLDDNKLQVTEFVTVSE
                     LANLMDVSFTDVISKCMGLGLMVSINQRLDAEVIELVAGEFGFEAEFTSIDDVEDEEE
                     DDEDTPENLAPRSPIVTIMGHVDHGKTSLLDYIRNASVVAGEAGGITQHIGAYEVTLA
                     NGKNITFLDTPGHEAFTAMRARGAKVTDIAVIVVAADDAVMPQTKEAISHAQAANVPM
                     IFAVNKIDKDGANPQKIYEQLSQMNLLVESWGGKYQSQELSAKKGLHINELLEKILLE
                     AELLELKANANREATGTIIEASLDKGRGYVATILVQNGTLKQGDIIVSGQYYGRIKAM
                     FNERNQRVQEAPPSTPVVILGLNGAPQAGEKFKVYSDEAYAKDIATKRAQIIREQGLR
                     ARKHITLDEIGRRLALGNFKELNIIIKGDVDGSVEALSDSLQKLSTSEVAVNVVLKAV
                     GQISESDVLLATASDAVLIGFNVRPSSQASKLAATEGVQIKQYSIIYTAIEEIKSAME
                     GMLEPKVEEKEVATVEIREVFRFDKAIVAGCYVTEGKIKRDHKIKVFRDGILVYPRQE
                     GAYAELGSLKRFKDDVKEVNNNYECGLTVKGFTDIQAGDTVVAYEEEEIKRTL"
     gene            6339..8606
                     /locus_tag="PLU36_02285"
     CDS             6339..8606
                     /locus_tag="PLU36_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HMZ45609.1"
                     /translation="MAHQANTDPEALSVAYNLNEEEEKKEILRRYRSLLKALRPKLKK
                     GNRQIVRNAFEMAADAHKTMRRKSGEPYIFHPIAVAMICVEEIGLGVRSTICALLHDT
                     VEDTDLTLEDIEREFGNEIAKIVDGLTKISTVFDNNTSQQAENFKKILLTLTDDPRVI
                     LIKLADRLQNMRTLEFMKREKQLKVSSETIWVYAPLAHRMGLYNIKTELEDLAMKYME
                     PEAYKDIAKRLAETKKERTKYINEFIKPLKEKLTNTGFNFEIYGRPKSIHSIWNKIKK
                     KDVTFNEVYDLFAIRIILNPPPEKEKEECWKVYSIITDEYNPNPERLRDWLSNPKSNG
                     YEALHTTVMGPQGKWVEIQIRTKRMNEIAEKGLAAHFKYKEGSNNEDRFDKWLGQIRE
                     ALSQDDTDSINFLKDFKTSFLAEEIYVYTPKGEVKMLPMGSTALDFAFAIHTAVGSNC
                     IGAKVHHKLVPISYKLKSGDQVEIITSSKQKPSEDWLNIVVTTKAKTKIKDALKEEKR
                     KIADDGKYVVQKKLEGLGAAWNTANIDELIVFYKLPSHLELFYKVAIKKIDLKELKEF
                     QVIGDRIEAPKTKIISKETITDLPTNKPSSKKDSELIIFGESSDKIQYNLAKCCNPIP
                     GDDVFGFVSSGKGLIIHRTNCPNATQLLANFGHRVVKTKWAKNKEISFLTGLKIIGMD
                     DVGVVHKITNIISGDLKINIAGLTIESSEGLFEGTIKVFVHDKEELDELVDRIKQLNG
                     IQKVDRFDMEEVANK"
     gene            8734..9111
                     /gene="rbfA"
                     /locus_tag="PLU36_02290"
     CDS             8734..9111
                     /gene="rbfA"
                     /locus_tag="PLU36_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HMZ45610.1"
                     /translation="MEEGKRQKQVAAVLQDELNNIFRKLGLNMMGGGMVSIAQVKVTP
                     DLLEARIYISFFKVEDIKAAMKKIDDKAWEIKRDLANHIKNQVRRIPVLHFYLDDTLD
                     YVFKMEDVFKKIETDKLNNNLAN"
     gene            9207..10430
                     /locus_tag="PLU36_02295"
     CDS             9207..10430
                     /locus_tag="PLU36_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HMZ45611.1"
                     /translation="MNFLFAWRYFKSKKSTNAVNIIAYISIVAIMIGTAALILVLSVF
                     NGFESLVKNLYNDFYADAKIVPVKGKSFHLNNQQYQLLQTNSNIAYWSKVVEEKALLK
                     NSENQATVIVKGVQNNYTNIAPINKHIIRGSFDIGNTENPKIVIGSGIESATAIYLEQ
                     PLEDPVLFLPNKKAVNLNSINNAFLSYNVIPSGTFVLQQDFDNKYIFTNLPFMQFMLD
                     MDSTQFSYLELKLTTKSSTDEEKIIKQLQQQLGTQVKVLSRYEQNKSLYTIMQIEKWI
                     IYGILCLILIVAAFNMIGSLTMLILEKQKDIAILKAIGANDVTIKNIFITNGFILAFV
                     GSFIGILLATLICWLQIKFKLLKLSGSFVVDYYPVEMRWQDYLLVLFTVFIIAFIASF
                     IPASKANRQNINLKS"
     gene            complement(10510..11085)
                     /locus_tag="PLU36_02300"
     CDS             complement(10510..11085)
                     /locus_tag="PLU36_02300"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HMZ45612.1"
                     /translation="MLNVILFGPPGSGKGTQSEKLIAKYGFKHLSTGDLLRSEITAKT
                     ALGLEAKGLMDKGQLVPDEVVIGMISHALEANPKATGFLFDGFPRTEAQSVALDNLLK
                     KYNTQIGVFLALEVSEEELVKRLLNRGLTSGRSDDTNEEVIRARIIEYERKTMVVADY
                     YEKFKKVAHIKGEGSVEDIFNTLCNEINKRV"
     gene            11175..11699
                     /gene="msrB"
                     /locus_tag="PLU36_02305"
     CDS             11175..11699
                     /gene="msrB"
                     /locus_tag="PLU36_02305"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HMZ45613.1"
                     /translation="MNIKFYFYTILLVLSFTNCTAQNNNNNKSNMQKENKANPYYSNK
                     DTNKLVLKEEEWRKILTSEQFYIARQKGTEQPFSSKFENFKEKGTYYCAACGNALFIS
                     DTKFESGCGWPSFYQPINKSSIIYTPDTTHGMQRTEVQCGRCKAHLGHVFNDGPPPSG
                     LRYCINGVILDFKK"
     gene            complement(11735..12433)
                     /locus_tag="PLU36_02310"
     CDS             complement(11735..12433)
                     /locus_tag="PLU36_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HMZ45614.1"
                     /translation="MLQLKEFLLLWWEIYFPVLTNPSLYKVSLGGTFYFSMFQYFIIY
                     KPFNTLSQFSSTENKQTLAHYCKLAKNIYPVGRLDYDSEGLLILTNDKKLNNLLLNPN
                     YEHRRTYIVQVEGSITPIAIQQLNNGVSINVNGKNYTTKKADAAIISAPINLPNRNPP
                     IRFRNNIPTSFIQLTLTEGKNRQVRKMTAKTGFPTLRLIRYSIENVSINNMQPGDIIE
                     LSQKTIYNKLSIPL"
     gene            complement(12810..14177)
                     /locus_tag="PLU36_02315"
     CDS             complement(12810..14177)
                     /locus_tag="PLU36_02315"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HMZ45615.1"
                     /translation="MQIWKEYQEQHKERFLNELLELLRIPSVSARSEHKEDMIKCAET
                     VKQRLLEAGADKVEIYPTAGHPVVYGEKIVDATKPTVLVYGHYDVQPPDPLELWHSGP
                     FEPVIKDGKIFARGACDDKGQFYMHVKALETMVNTNTLPLNVKFLIEGEEEIGSMHLG
                     EFLKQHKELLQSDVILISDTAMLSMENPSIDVGVRGLTYIEVEVTGPNRDLHSGVYGG
                     AVANPLTILAKMISSLHDENNHITIPGFYDDVVESSEAERKQLAEAPYDEEEYKKDLG
                     VKALWGEKGFTTNERTGIRPTLEVNGLWGGYTGEGAKTVLPSKAFAKISCRLVPNQTT
                     AKTEKLLIDHLHKIAPDYIQLKASLHHGGDPYMTPVNSIEYKAAAKAIEASFGKAPIP
                     VRGGGSIPITALFESVLGVKTVFMGFGLDSDNLHSPNEKYDLYNYYKGIETIPFFHQF
                     YNEMK"
     gene            complement(14258..15154)
                     /locus_tag="PLU36_02320"
     CDS             complement(14258..15154)
                     /locus_tag="PLU36_02320"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMZ45616.1"
                     /translation="MKKTYSKWILFVVLCLIWGSSFMLMKWGMFDKNNNPTLSAYDVA
                     ALRILFAGIVMLPFVPSSIKKISSKYLVYVLLSGWLGSFFPAFLFCIAESKIDGSLAG
                     SLNALTSLFVIITGFLFFNVKSTRKKIIGVIIGLLGCTLITVANYSKPFEYIAYSGFV
                     IFATFMYGFNINLIQKKLSALNALSVAAISLTGLIIPSATVLFFSGYFSLPLLQHTYI
                     ISTLSSVVLGILGTSLATILFYVLIKRSGGLFASLVTYGIPFVAIGWGFYNNEFITHV
                     HIIALLVILIGVFIVNKKQPKL"
BASE COUNT         5893 a         2213 c         2494 g         4767 t
ORIGIN      
        1 atcttgaata ataaaatttg atttatttaa tggctgttcc cctctttcta atttggtaat
       61 ctcatctaaa tcatttacta aattaaccat cctttctaca ttgtttgcag cattatttaa
      121 aaacttctgg tttgtttcat tatcttttaa accctcagcc tgtaaagctt ctatgtaacc
      181 ttgaatggca aaaatgggtg ttttaaattc atgcgaaata ttttgcaaaa actcttttct
      241 aaaagcctcg ttttgtttta aaagttcaat ttctgctttt ctctgatctg cccatttttc
      301 aacatcttct tttacttcat caatactttt tttaggtaaa atatatttat aataggtttc
      361 ttctttttta ctagcctttg tattaaaaat aaatttataa atcagtttta ttttacgata
      421 aataaaccat tctaaaacaa aactaattaa aaaatagctg cctataaaca tcactacaaa
      481 agcagcaaat cctatccaca cttgcttttc aataacaaag aaaaccaagc tggctggtaa
      541 agaaactata aaagaggtaa aagcagataa ctgttttggg ctaaggtttt tggtagtaaa
      601 catagtgcta cgaaactact atgtttttaa ttgataaaat aataataata aacaaactat
      661 tgttaagttt tataaagtag caatcctttt tacttgcata aaactttacc aggtaattgc
      721 caattttgtt ttggtattat cccaggctat aattaaatct gcaccgtttt ttaatggttc
      781 aaaatatata ctaagggctt ctatggcttc ttctgttttt tctagtgtac aatctaccct
      841 aagtaaatct ttttttgcat cgtaattaaa actgccccaa gagtagttat ctttattaaa
      901 aataatagtc catttagttg gttgaggaat acataataaa ctatacctgc cttttgcaat
      961 agttttacca gcaattttta catttctaaa aaactcaatt tctgtagctt catttgctcc
     1021 taacctccat atttctttgt attgaattat tccgccaaat atttcacgcc ctttcttcat
     1081 tggtctgcta taaattactc tggcaatggg taaatcttta cttttaccat tcattttcat
     1141 taatggataa tctttaggaa aatagctaat atccattggg cttttatcaa tttctggaac
     1201 tttggctgct tcttgagcta ttaaatcttt tgtaataaaa agaaaactaa atacaaaaac
     1261 aacacaaatt ttacatatat ttttaatcat agtacaaaac tttatgcaaa aatataatta
     1321 tatacttagt taaatacttt taagaaaatt tgaagaaaat tttaagagta ataaaaattt
     1381 ggaacataaa taattattat gctacatttg caacggaaat tgaaacagat gaaaggaacg
     1441 aggtcgttcc tttctttttt atacctatgt cagcagattc taatttaaac aagattgaaa
     1501 tttttattaa tggcttatta caaagcgagc caaactactt tttggttgat ttaaaggtaa
     1561 agccaaccaa taatataaaa gtatttattg atggcgatga aggaataacc attgaaaaat
     1621 gtatttattt taatagaaaa ttatacaagc taatagaaga agcaggtatt tacgaagaag
     1681 gtaattttag tttagaaatt tcgagtccgg gagtaggaga accattaaaa ttaaaaagac
     1741 agtatcaaaa aaacattggt agaaatatag aagtaatttt tatagatgaa agcaaaaaag
     1801 atggtaagct tttagatgtt atggaagact ccatcttagt agaaaacaca acaggaaaag
     1861 ggaagaaagc agttacccaa caattagtaa tttctttttt aaacataaaa acaacaacag
     1921 ttcaaattaa attttagcta gaagaagcaa ctttttgctt cattttttaa atttaaatta
     1981 tatgacaagg ataaacttaa ttgatgcctt tcaggatttt aaagatgcag agaatcttga
     2041 cagaccaacc ttaatgaagg tggtagaaga tgtatttaaa acacttctaa gaaaaagata
     2101 tggtgcagat gataattttg atgtaatagt taatgccgaa aaaggcgatt tagaaattat
     2161 tcgaagaaga atgattgtgg aagatggtga agtactagac cccttagcag aaattggata
     2221 tactgaagct accaaaatag acccagattt tgaagtaggc gaagatcttt acgaagaagt
     2281 aaatatgatt gattttggac gaagagcaat tttagctgcc aaacaaaccc tggcaagccg
     2341 tataaacgat ttaaagaaaa acgtattggt aaaaaaatat gccgatagaa ttggggaaat
     2401 tattagtgca gaagtttatc aggtttggaa gaaagaagta ctattgttag atgaagatgg
     2461 aaatgaatta attttaccaa aatctgaaca aataccacaa gactttttca aaaaaggaga
     2521 aaatattaga gctgttgtag aaaaagtaga tttaaaaaat aatgctccag taattattct
     2581 ttcaagaaca agtcataaat ttttagctaa gcttttagaa attgaggttc cagaaatttt
     2641 tgatggttta attactatta aaaaaattgt tagagaacct ggagaaagag ccaaagttgc
     2701 tgtagaaagc tacgacgata gaatagaccc tgtaggtgct tgtgttggta tgaaaggtag
     2761 cagaatacat ggaatagtta gagaactaaa aaatgaaaat atagatgtaa ttaactacac
     2821 aacaaatatt caattactta ttcaacgttc tttaacacct gctaaaatta gtttcatgaa
     2881 tttagataac gacaagaaac aagcagatgt atatttaaag gcagatcaag tatctttagc
     2941 cattggcaaa agaggcgtaa atattaaatt ggcttgcgaa cttaccggtt atgaaataga
     3001 tgtttacaga gaagatgaag aggaacaaga atacgaattt gatatagatt tagatgaatt
     3061 tagcaacgaa atagaacaat ggataattga cgaattaaaa cgtgtaggtt gcgatactgc
     3121 acaaagtgtt ttaaaattat ctaacataga aattgaaaga agaacagatc tggaaaaaga
     3181 aactatagaa gaaataagaa gcattttaca agcagaattt gataaagaag cagagtaaaa
     3241 tagtaaaccc aaagcagata aaaatctgtt ttgaaataat aaacaaaatt tcgctaatac
     3301 agaagaacag aaaaacaata acttaatgtc tgaattgaaa ctaccaagat tattagcagc
     3361 agctaaagaa tttaatatag ggcaagaaac ccttatagat tttttagcaa aaaaaggatt
     3421 taataaagac gatctgaagc caactgcaaa gctttcagaa gaaatgtatt atgctttaca
     3481 agccgaattt caaagtgata aagccgcaaa aaacaaagca gaccaaatag aaattccaaa
     3541 atctgttgca ccagataaaa agaaaaaaga agaagagact gtaacaaaaa aggtagagaa
     3601 aaaagccgaa gaaataaaag tagagccttc tccaattaaa gaagaaccat tagaagaaaa
     3661 aaagcctgat gaagtaaaaa aagttgaagt agaagtagag aaaaaaattg aacaaaaaac
     3721 aacagcaaca gaaatagttg ctcctaaagt tgttgataaa atagatttat ctgcaataga
     3781 ttcatctacc agaccaaaga aaactgccaa agcaaaaaca actgaaccaa ccaaagaaca
     3841 accttcaagt aaaaaagaag aaactgcaaa agtagaacag caagaaccaa aagtggaacc
     3901 tgctattaca gaggagacta acagcattgt aaaaccaccg gatacagcaa gtacaattgt
     3961 agaaaatatt gaagttgaga aattaacagg gccaaaaata ttaggcaaaa ttacattacc
     4021 tgtagatagt gatacaaggc caaaaccaat atcaaaagta gaacagcata aacgtaaacg
     4081 tatagttgta gataaaaagc caggtagcaa tatacaacgt gtagatttta gaaaagaagg
     4141 aggagggcaa acacaaacag gtaataataa ccgtggtgga aaccagcaac agcaaaatag
     4201 atttggagga aaaccaggag gtggtggagg tcatccacaa agaggaggag gaagaaatgc
     4261 tggaaataat agaggcaaac aagataatcg tagagtagca gataaagaaa ttgatgcaaa
     4321 agaaattcag gataaaatta aagaaacaca agcaaaactt gcaggtagtg gaggtagagg
     4381 taaaagccta aaagcaaaat acagaagaga aaagaggcaa gaagctgcag aagctgccat
     4441 ggaaggaagt ggattagatg ataataaatt acaagttaca gagtttgtta cagtaagtga
     4501 actagctaac ctaatggatg taagctttac agatgtaatc agtaaatgta tgggcttggg
     4561 cttaatggta tcaataaacc aaagattaga tgccgaagtg attgaattag ttgccggaga
     4621 gtttggtttt gaagctgaat ttaccagcat tgatgatgta gaagatgaag aggaagacga
     4681 tgaagatacg ccagaaaatt tagctccaag gtctccaata gtaaccatta tgggacacgt
     4741 agatcatggt aaaacttcat tacttgatta tatcagaaat gctagtgtag tagcaggaga
     4801 agcaggtgga attacacaac atattggtgc ttatgaggta acattagcta acggaaaaaa
     4861 tataacattt ttagatacac caggtcacga agcttttaca gctatgaggg caagaggtgc
     4921 aaaagtaacc gatattgcag ttattgtggt tgctgcagac gatgcagtaa tgcctcaaac
     4981 taaagaagca atcagccatg ctcaagcagc caatgtacca atgatttttg ctgtaaataa
     5041 aattgataaa gatggagcaa atcctcaaaa aatttatgag caattatctc aaatgaattt
     5101 attagtagaa agttggggag gtaaatatca gagtcaggaa ttaagtgcaa aaaaagggct
     5161 tcatataaat gaacttttag aaaaaatatt attagaagca gaattattag aactaaaagc
     5221 caatgcaaat agagaagcta caggaacaat tattgaagca tctttagata aaggacgtgg
     5281 ttatgttgca acaattcttg ttcaaaacgg tactttaaaa caaggcgata ttattgtatc
     5341 tggtcaatat tatggtagaa taaaagccat gttcaacgaa cgtaatcaac gtgttcaaga
     5401 agccccacca tctacccctg ttgtaatatt aggattaaat ggtgcaccac aagcaggaga
     5461 aaaattcaaa gtttattccg atgaagctta tgcaaaagat attgccacca aaagagctca
     5521 aataattaga gagcaaggct tgcgtgcaag aaaacatatt actttagatg aaataggcag
     5581 aaggcttgct ttaggtaatt ttaaagagtt aaatattatt attaaaggag acgtggatgg
     5641 ttccgttgaa gcattaagcg atagcttaca aaaactttca acatccgagg tagcagttaa
     5701 tgtagtttta aaagcggttg gtcaaatttc agaaagcgat gtacttttag ctacagcttc
     5761 tgatgctgtt ttaattgggt ttaatgttcg tccatcttct caagcaagta aactagcagc
     5821 taccgaagga gtacaaataa aacaatactc tattatttat actgcaattg aggaaattaa
     5881 gagtgctatg gaaggtatgt tagaacctaa agtagaagaa aaagaagttg ctactgttga
     5941 gataagagaa gtatttagat ttgataaagc tattgttgct ggttgttatg taacagaagg
     6001 aaaaattaag agagaccata aaattaaagt atttagagat ggcattttag tttatccaag
     6061 acaagaaggt gcttatgctg aattaggaag cttaaaacga tttaaagacg atgtaaaaga
     6121 ggtaaacaac aattatgaat gcggtttaac tgtaaaagga tttacagaca ttcaagcagg
     6181 cgatactgtt gttgcttacg aagaagagga aattaaaaga acactataac tataaatagt
     6241 ataaatattt aagaggctga ttactcagcc tctttttttg ttttaacaat agccttaaaa
     6301 aatatttacg taaaaaaaat tcatatattc gttacactat ggcccatcaa gcaaatacag
     6361 acccagaagc actctctgtt gcatataatt taaatgaaga agaagaaaaa aaagaaatat
     6421 taaggcgtta cagaagcctc ttaaaagctt tacgcccaaa acttaaaaaa ggtaataggc
     6481 aaattgtacg caatgctttt gaaatggcag cagatgccca taaaactatg cgtagaaaaa
     6541 gtggcgaacc ttatattttt catcctattg cagttgctat gatttgtgtg gaagaaattg
     6601 gattaggagt aagaagcacc atttgtgcat tactgcatga tacagtagaa gacacagact
     6661 taaccttaga agatattgaa agagaatttg gaaacgaaat agcaaaaatt gtggatgggt
     6721 taacaaaaat ttcaactgta tttgataaca acacctctca acaagcagaa aattttaaaa
     6781 aaattcttct aaccttaaca gatgacccaa gagtaatatt aataaaatta gcagacaggc
     6841 ttcaaaatat gcgaacccta gaatttatga aaagggaaaa gcaattaaaa gtatctagcg
     6901 aaactatttg ggtatatgca cctcttgctc atagaatggg cttatataat attaaaaccg
     6961 aactagaaga ccttgcaatg aaatatatgg agccagaagc ctataaagac attgcaaaaa
     7021 gattagcaga aactaaaaaa gaaagaacaa aatatatcaa tgaattcata aaaccattaa
     7081 aagaaaagtt aactaataca ggatttaatt ttgaaatata tggtaggcca aaaagcattc
     7141 actcaatatg gaataaaata aaaaagaaag atgtaacatt taatgaagta tatgatttgt
     7201 ttgcaattag aataattcta aatccaccgc cagaaaaaga aaaagaagaa tgctggaaag
     7261 tttatagcat tattacagat gaatacaacc ctaacccaga aagattaaga gattggctaa
     7321 gcaacccaaa aagcaatgga tacgaggctt tgcatacaac agtgatgggc cctcaaggaa
     7381 aatgggtaga gatacaaata cgaactaaaa gaatgaatga aattgcagaa aaagggttag
     7441 ctgcacattt taaatacaaa gaaggcagca ataatgaaga tagatttgat aaatggttag
     7501 ggcaaattag agaagcactt tctcaagacg atacagatag tataaacttt ttaaaagatt
     7561 ttaaaacatc atttcttgcc gaagaaattt atgtttacac tcctaaaggc gaagtaaaaa
     7621 tgttaccaat gggcagtaca gcattagact ttgcatttgc aattcatact gctgttggta
     7681 gtaactgtat tggtgctaaa gttcatcata aactagtgcc aatttcctat aaacttaaaa
     7741 gtggagatca agtagaaatt attacaagct ctaaacaaaa accatcagaa gattggctaa
     7801 atattgttgt aactacaaaa gcaaaaacaa aaattaaaga cgcacttaag gaagaaaaaa
     7861 gaaaaattgc agacgatgga aagtatgtag tgcaaaaaaa gttagaaggc cttggtgcag
     7921 catggaatac agccaacatt gatgagttaa tagtatttta taaactaccc tctcaccttg
     7981 aattatttta taaagtagct ataaaaaaaa tagacttaaa agaactaaaa gagtttcagg
     8041 ttataggcga tagaattgag gctccaaaaa caaaaattat ttctaaagaa accattaccg
     8101 atcttcctac aaacaaacca tcttctaaaa aagatagtga actgattatt tttggcgaaa
     8161 gcagcgataa aatacaatat aatttagcca aatgttgtaa cccaattcct ggcgatgatg
     8221 tttttggttt tgtaagctct ggcaaaggtt taattattca cagaactaat tgccctaatg
     8281 ccacccagtt attagctaat tttggacata gagtagttaa aacaaaatgg gcaaaaaata
     8341 aagaaatatc ttttttaact ggcttaaaaa ttattggtat ggatgatgtt ggtgtggtac
     8401 ataaaattac caatattatt agtggcgatt taaaaataaa tattgctggt ttaactatag
     8461 aaagtagcga aggtttattt gaaggaacca taaaagtatt tgtacacgat aaagaagaac
     8521 tagatgaatt agtagataga attaaacaac taaatggtat tcaaaaagta gataggtttg
     8581 atatggaaga ggtagccaac aaataatgtt aatacaggct acatcataaa aagagtaaat
     8641 atttcttact taaaattttg tctcttagcc tctatcttat tttcaatact taccaactaa
     8701 attaaaggtt gtacttttgt aaaaattaaa tgtatggaag aaggaaaaag acaaaagcag
     8761 gtagctgctg tactgcaaga tgaactaaat aatattttta gaaaattagg cttaaatatg
     8821 atggggggtg gtatggtatc tattgcccaa gtgaaggtta ctccagattt attagaggca
     8881 agaatttata ttagtttttt taaggttgaa gacattaagg ctgcaatgaa aaaaattgac
     8941 gataaagctt gggaaattaa aagagattta gccaatcata taaaaaatca agttaggcgt
     9001 attcctgtat tacattttta tttagatgat acacttgatt atgtttttaa aatggaagat
     9061 gtatttaaaa aaatagaaac cgataaattg aataataatt tagccaacta atgtacaaag
     9121 ccctgaagtg tgcgacgcaa caaatgaata atagttttac aaaagcttag tacaaaaaaa
     9181 taattaatct gtaaatttta tagctgttga actttttatt tgcatggcgt tattttaaaa
     9241 gtaaaaaaag taccaatgcc gtaaacatta ttgcatacat aagtattgta gcaattatga
     9301 ttggcactgc agccttaatt ttagtgttaa gtgtttttaa tggatttgaa agtttagtta
     9361 aaaacttata caacgatttt tatgcagatg ctaaaattgt tcctgtaaaa ggtaaaagct
     9421 ttcacttaaa caatcagcag tatcaattac tacaaaccaa ttcaaatatt gcatattgga
     9481 gtaaggtagt agaagaaaaa gcactattaa aaaatagcga aaaccaagca actgtaattg
     9541 taaaaggtgt tcaaaataat tataccaata ttgcaccaat aaataaacat ataattcgtg
     9601 gtagttttga tattggcaat acagaaaacc ctaaaatagt aattggtagt ggcattgaaa
     9661 gtgctactgc aatttattta gagcaaccat tagaagaccc tgttttattt ttaccaaaca
     9721 aaaaggcagt aaatttaaat agtataaaca atgctttttt atcttataat gtaataccaa
     9781 gcggcacttt tgttttacag caagattttg ataataaata tatttttacc aatttacctt
     9841 ttatgcagtt tatgctagat atggatagta ctcagttttc gtatctagaa ctaaagctta
     9901 caacaaaaag cagcaccgat gaagaaaaaa ttataaagca acttcaacaa caattaggca
     9961 ctcaagttaa agtgctatcc agatacgagc aaaacaaaag tctttatacc ataatgcaaa
    10021 tagaaaaatg gattatttat ggtattcttt gcttaatatt aattgttgca gcttttaaca
    10081 tgattggttc attaaccatg ctaatattag aaaaacaaaa agacattgct attttaaaag
    10141 caattggtgc aaatgatgta accataaaaa atatttttat caccaatggt tttattttag
    10201 cctttgtggg ttcattcatt ggtattttat tagctaccct aatttgttgg cttcaaataa
    10261 aatttaagct actaaaactt agcggaagtt ttgtagtaga ttattaccct gttgaaatgc
    10321 gttggcaaga ttatttatta gtgctattta cagtttttat tattgcattt atagcttcat
    10381 ttattcctgc aagtaaggca aacaggcaaa atataaattt aaaaagttag taataaaaaa
    10441 atcctactaa gaatactcag taggattggt tgcagttggt attggtagta aaaattaagg
    10501 atttataatt tatacccttt tattaatttc attgcatagg gtattaaaaa tatcttcaac
    10561 actgccttcg ccttttatat gtgctacttt tttaaatttt tcataataat ctgccaccac
    10621 cattgttttt ctttcatact caataattct ggctcttatt acttcttcat tggtatcatc
    10681 acttctgcca ctggttaatc ctctattcaa caatctttta accaactctt cttcgctaac
    10741 ttctaaggca agaaaaacgc caatttgtgt attgtatttt tttaataaat tatctaatgc
    10801 tacactttgt gcttctgttc ttggaaaacc atcaaataaa aagcctgtag cttttggatt
    10861 ggcttctaat gcatggctta tcataccaat tacaacttcg tctggcacca actgaccttt
    10921 atccattaag cctttagcct ctaagcctaa tgctgttttt gcagtaattt cacttcttag
    10981 caaatcgcct gtacttaaat gcttaaagcc atatttagca ataagttttt cactctgtgt
    11041 gcctttgcca ctacctgggg gaccaaataa aataacattt aacatgtacc taaaacctta
    11101 cttgaacaac aaatataagg cttgagtgtt aattttgtaa acaataaaaa aattagataa
    11161 attattattc aaatatgaat attaaatttt atttctatac cattttactt gttttgtctt
    11221 ttactaattg tacagctcaa aacaataata ataacaaaag caatatgcaa aaagaaaata
    11281 aagcaaatcc atattacagt aataaggata ccaataaact tgtattaaaa gaagaagaat
    11341 ggagaaaaat tttaacttct gaacagtttt atattgcaag gcaaaaaggc actgagcaac
    11401 cttttagtag taagtttgaa aattttaaag aaaaaggaac ttattattgt gcagcttgtg
    11461 gtaatgcact ttttataagt gataccaaat ttgagagtgg ttgtggttgg ccaagttttt
    11521 atcaaccaat taataagtct tctattattt atacgccaga taccacacat ggaatgcaaa
    11581 gaacagaagt acagtgtggc agatgcaaag ctcatttagg tcatgttttt aatgatggtc
    11641 ctccaccatc ggggttaagg tattgcatta atggagtgat tttagatttt aaaaaatgat
    11701 ttataaaaat tgagtaacaa attgagtaac ttaattataa aggaattgaa agtttattat
    11761 aaattgtttt ttgggataat tcaattatat ctccaggctg catattatta atagatacat
    11821 tctctatact ataccttatt aaacgcaaag ttggaaatcc tgtttttgca gtcatttttc
    11881 ttacctgcct atttttacct tcggtaagcg ttaattgtat aaaactagtt ggtatattat
    11941 ttctaaatct tatgggagga tttctatttg gtaaattaat tggtgcacta attattgcag
    12001 catctgcttt tttagttgta taatttttac cattaacatt aatacttaca ccattgttta
    12061 attgttgtat tgcaataggt gtaatgctac cttctacttg tacaatataa gttcttctat
    12121 gttcgtaatt tggattaagt aataagttat ttaatttttt atcgttggta agaattaata
    12181 aaccttcact atcataatct agcctaccaa caggataaat attttttgca agcttacaat
    12241 aatgtgcaag tgtttgtttg ttttctgttg aggaaaactg gctgagtgta ttaaaaggtt
    12301 tatatataat aaagtattga aacatgctaa aataaaaagt gccgccaagc gacactttat
    12361 ataatgatgg gttagtaagt actgggaaat aaatttccca ccacaataat aaaaattctt
    12421 ttaactgtaa caaaaaaaaa tatatacaaa cttatcaaca ttttattttt ttttgtgaat
    12481 tgacagaaga attactttat taattatttc atacttcagt actcatttta tgaaaatata
    12541 tttctactgt taatactaca acactttgaa acacttacaa ataaagggct acaaaggatt
    12601 atgagatttt attttataaa catgaatcaa aaagaataaa aaaacttttc actgaaattc
    12661 taaaaatatt tttctcaact tctaaaaaaa aaataaaaaa aaggttcatg tacctattga
    12721 ataaattaaa aaacagagaa gaaaaaattc aagcaacaaa aaatcctgat gtagttatca
    12781 tcaggatttt taaaattata aattaaaaac tatttcattt cattgtaaaa ttgatgaaag
    12841 aaaggaatag tttcaatgcc tttataataa ttatacaaat catatttctc gttagggcta
    12901 tgtaaattat cactatctaa tccaaagccc atgaaaacag ttttaactcc aagaacactt
    12961 tcaaacaatg cagttattgg aatactacca ccaccacgaa caggtattgg tgctttgcca
    13021 aaacttgctt caattgcttt agctgcagct ttgtattcta tactgtttac aggagtcatg
    13081 tatgggtctc caccatgatg taagcttgct tttaattgaa tatagtctgg tgcaatttta
    13141 tgcaaatggt ctatcaataa tttttcagtt ttagcagtcg tttggttggg tactaaacgg
    13201 caactaattt tagcaaaagc tttacttggt aaaacagttt ttgcaccttc tcctgtatag
    13261 ccaccccata aaccatttac ttctaatgtt ggtcttatgc cagttctctc attggttgta
    13321 aatccttttt cgccccataa tgcttttaca cctaaatctt ttttatactc ttcttcatca
    13381 taaggagctt ctgctaattg ttttctttca gcttcgctgc tttctaccac atcatcataa
    13441 aaaccaggta tagtaatatg attattttca tcgtgtaaag aagaaatcat tttagctaaa
    13501 atagttaatg ggtttgcaac tgcacctcca tacacaccac tatgtaaatc tctatttggt
    13561 cctgttactt ctacttcaat ataagttaag cctcttacgc caacatcaat gcttgggttc
    13621 tccatgctta acatagcagt atcgctaatt aaaataacat ctgattgtaa aagctcttta
    13681 tgttgtttca aaaattcgcc taaatgcatg ctaccaattt cttcttcccc ttctattaag
    13741 aattttacat ttaaaggtaa agtgttggtg ttaaccattg tttctaatgc tttaacatgc
    13801 atataaaatt gtcctttatc atcacaggct ccacgagcaa aaatttttcc atctttaata
    13861 actggttcaa atggcccact atgccaaagt tctaaagggt ctggtggttg aacatcgtaa
    13921 tgcccataaa ccaaaactgt aggtttggtt gcatcaacaa ttttttctcc atacactaca
    13981 ggatgtcctg ctgtgggata aatttcaact ttatctgcac ctgcttctaa taatctttgc
    14041 tttacagttt cggcacactt aatcatatct tctttatgtt cacttcttgc acttacacta
    14101 ggtattctta ataactccaa caactcattt aagaaccttt ctttgtgttg ttcttgatat
    14161 tctttccaaa tttgcattgc taaaaatttt tgttgaacaa cgaaagtagg aatgtttttt
    14221 tagtgtaaaa aaaaactata aaacaaggta actggtatta caatttaggc tgttttttgt
    14281 ttacaataaa aacgcctata agtataacca ataaagcaat aatgtgcaca tgggtaataa
    14341 actcattatt ataaaaaccc caacctattg ctacaaaagg aattccataa gttaccagtg
    14401 aagcaaataa gccaccagat cttttaatta atacataaaa taaaatagtt gcaagcgaag
    14461 tacccaatat gcctaaaact acacttgaca aggtactaat tatataagta tgttgtaaaa
    14521 gtggcaatga aaaataacct gaaaaaaata aaacagttgc tgaaggaatt atcaaacctg
    14581 tgaggctaat agcagcaaca gataaagcat ttaatgcgga taattttttt tgaattaggt
    14641 ttatattaaa gccatacata aaggtggcaa atattacaaa accactgtat gctatgtatt
    14701 caaaaggttt gctgtaattg gcaactgtaa ttaaagtgca tcctaataat cctattatta
    14761 caccaattat ttttttacga gtactcttta cattaaaaaa caagaagcct gttataatta
    14821 caaacaaaga ggttaaagca tttaaacttc ctgccaaaga gccatcaatt ttagattcag
    14881 caatgcaaaa taaaaatgct ggaaaaaaac ttcctaacca tccacttaat aaaacatata
    14941 caagatattt acttgaaatt tttttgatac tacttggaac aaatggaagc attacaattc
    15001 ctgcaaacaa aattcttaaa gcagctacat cataagcaga aagagtaggg ttgttgtttt
    15061 tatcaaacat accccatttc attaacataa aagaactacc ccatattaaa catagcacta
    15121 caaataaaat ccatttgctg taagtttttt tcacaaaata aatttaagca aaggaagagt
    15181 aatttataaa taagttgaat tttattctac cgtaattatt ttagattgta tgttattgcc
    15241 aatatctaca actattatgg agtatatacc tttaggaagt gatgaaatac cgagttgtac
    15301 gttattcaaa atattatagt tattagttat caatttcctt cctattatga ctgcaacagt
    15361 tacttgt
//
LOCUS       DAOQPK010000026        15232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_1978_length_15232_cov_5.105554, whole genome shotgun sequence.
ACCESSION   DAOQPK010000026 DAOQPK010000000
VERSION     DAOQPK010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 15232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15232
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1978_length_15232_cov_5.105554"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1393
                     /locus_tag="PLU36_02325"
     CDS             149..1393
                     /locus_tag="PLU36_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ45617.1"
                     /translation="MKKLFHKEGLSPLKYKEFRLFIAFRFLYIMALRMVSTVVAYELF
                     QITQSSFSIGLVGLSEFIPVFSLALYAGHVIDKSDKRTMLMKGMMLYAICVVGLIVVT
                     HSSMKKHLSVTAMEMCFYTIIFFTGVIRAFVGPSNNAIIFQLVPKSILHFASNLSSST
                     FLTASIVGHASAGFYIAWFGVNTTFVLILFFVIASVFIVSKIIKKPITNTKLDVKTWD
                     SMKEGLLYVFKNKILLGAISLDLFAVLFGGAVALLPEFASKILRVGPIGFGWLNAATD
                     IGSMLIIISLTLFPLRKRQGEIMLYAVACFGICIMLFGLSNFYTVSFILLLLAGCFDG
                     ISVIVRSTILQLTTPDEMRGRVSSVNSMFINSSNELGQLESGIASRLLGLRPSIIFGG
                     SVTLIVVIITWFKAKQLRKMQY"
     gene            1441..2475
                     /locus_tag="PLU36_02330"
     CDS             1441..2475
                     /locus_tag="PLU36_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HMZ45618.1"
                     /translation="MTLQQNNKAVATWLLVGAAMIIIQVLLGGLTRLTDSGLSITEWQ
                     PLLGSIPPLNNTDWQIAFNKYKELAQFKFLHSHFNLNDFKFIYFWEWFHRIWARLMGV
                     VFILPFVYFIYKKWINRTMLMPMIILFLLGGLQGFVGWFMVKSGVGTSLVFVDHTRLA
                     IHFIAALVLLVYVVWFALKLIINNNQLINAPKIKRLNILIIALLTIQLIYGAFMAGSR
                     AAYSAITWPSINGFFYPPGMLHKESLWYDLFNNLLAIQFIHRGLAYLLLIVIIIFTLQ
                     LKKINSSKFLNSIKFLPFYLVTVQVILGIFTIINYTTKYKLILSITHQFIGMLLLVSL
                     FFTLFIVRKK"
     gene            2551..3198
                     /locus_tag="PLU36_02335"
     CDS             2551..3198
                     /locus_tag="PLU36_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HMZ45619.1"
                     /translation="MNLYYCYDAYCGWCYGFSPIIKKIAEEYKNVLHTEVLSGGMILP
                     ETPTHIGTMANYILKAYKTVEEYAGIKFGKDFLWHIENPTLSDWFPDSLKPAIALCIF
                     KEYHANLQIQFASDLQYALNFEGRDLTDDAAYLHLLTKYNIPKNAFYEKMNSEEYIDK
                     AKYEFSLCKQLQVTGYPCVLLQANESKFYLLAKGFTTYEELKKRVENVLNELTAN"
     gene            3204..3680
                     /locus_tag="PLU36_02340"
     CDS             3204..3680
                     /locus_tag="PLU36_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HMZ45620.1"
                     /translation="MDKNIETIQKFYTAFQQKDYETMNSCYANDIAFSDPIFGLLHGN
                     QVKAMWQMLCTNAKEFKLQFNNIKTDDDEYYTCSWIAMYRFSATNRRVVNKCTAYMRL
                     KDGVIIEHSDGFNYYKWCRQAFGIKGILLGWSGFMHKKVSNKARKQLFKYMEQHGL"
     gene            3749..4027
                     /locus_tag="PLU36_02345"
     CDS             3749..4027
                     /locus_tag="PLU36_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HMZ45621.1"
                     /translation="MKEHKSHTIDTASSTKKITSLFGKENYILMAVGAIIITVGMLLM
                     SGGKNQDPNVFDESVVYSTTRITVAPILILIGFVVEIYAIFKKPKASA"
     gene            4124..5323
                     /locus_tag="PLU36_02350"
     CDS             4124..5323
                     /locus_tag="PLU36_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45622.1"
                     /translation="MTFNDVIGNKNVKQHLLELMLQNRLPHALLFLGKEGSGALPLAL
                     AFAQLIICQPKSSPVVEDLFGGMSAISNEPSFLHPNDIESSTSYSKANLLVHPDLHFS
                     FPFINKKNSDSSTISADYITEWREFLMQSPYGNLYDWLQVIKAENRQGNISAKECLEI
                     IKKLSLKSFESDYKVLLMWLPELLSKEGNKLLKLIEEPPPNTIFIFVAENEEKILPTI
                     LSRCQLIKIPLPENDEVEEALLKKANIEPEQAKQIAAISNGNYREALHLIQHAEEDWQ
                     GLLRDWLNTIMRRLLPENIKWIEEISKIGREKQKQLLQYFNHTIEQALRLKTIGADNI
                     SLSESEKDFAQRFNKIANISQMQAISEELDKATFSIERNVNGKIVFTALSVKLYHILI
                     NKVVVEV"
     gene            5457..6650
                     /locus_tag="PLU36_02355"
     CDS             5457..6650
                     /locus_tag="PLU36_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HMZ45623.1"
                     /translation="MSNTLQSNESISSSRKKIIVLGSGPNRIGQGIEFDYCCVHGLLA
                     IKECGYEAIMINCNPETVSTDFDMADKLYFEPVYWEHLWEIIELEKPEGVIVQLGGQT
                     ALKLAKKLHEKGIKIIGTSYNDMDIAEDRGRFSDLLKELEIPYPNYGTAYTAEEAIEV
                     ANKVGYPVLVRPSYVLGGQRMRIVINDEEVEKSCISILKHIPDNKILIDHFLDRCQEA
                     EVDAIYDGKSFHVMGIMEHIEPAGIHSGDSNAVLPAFNLTPLVIETMQFYSEKIAKAL
                     NIKGLINIQFAIKDGKVYVIEANPRASRTTPFIAKAYQIPYLNIATKVMLDKAKLSDF
                     NLEKKLDGYAIKEPVFSFNKFPGVNKELGPEMKSTGEAIRFIKDLRDPYFRTLYKERS
                     MHLSK"
     gene            6773..7897
                     /locus_tag="PLU36_02360"
     CDS             6773..7897
                     /locus_tag="PLU36_02360"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HMZ45624.1"
                     /translation="MRLLLMLAILIVFFTTVTAQKVFKDANGSYGLKDNNGKIILKPT
                     YTFIRSFVDGFAAIAINKYPNIKWSFINTKGQIITPFKYDKIDFFSEGLAVVKLNKLY
                     SYINSLGKEVFGFIFLEAFGFHQNIAVVKNKNGKYGVINKQGKTVVNYKYDNMNSHFT
                     EGLAWVTLNNKVGFINAKGIEVAACIYDDAGNFNEGMAPVKQNGKWGFINNKGKVVIP
                     FQYDVTSLNSFNNGLVKVKINEYYGFINKQGEEVIPFIYENVDDFSEGLAGFLKEGKY
                     GFMDLTGKEIIKPIYKYTWGFNNGLAAVEVENEKKENKWGFINKKGILVIPALYSSIL
                     PQQNKHTHKIIFGFYKNKAYVSLSDKNNFYINNKGLKISD"
     gene            7975..9336
                     /locus_tag="PLU36_02365"
     CDS             7975..9336
                     /locus_tag="PLU36_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HMZ45625.1"
                     /translation="MKKILLLLLLPIVANSQQTDSLFIKKIADEILTNGKAYDLLYHL
                     TKKIGGRLAGSPQYNKAVNWGFNTMQQMGADKVFLQECIVPHWVRGVGDFATLQNTNS
                     SSVKKQLNITAIGNSLGNTKIIKAPIILIHNFQELENKKEEVKGKIVFYNAAFNPTNI
                     RTFKSYGETASYRVNGASRAAKYGAVAVIVRSLTEATDNHPHTGTLRYNDSFPKIPAV
                     CVGLKDADYIAQNIKKGNWQVALKTNGKLLPDTINHNVIAELKGTEFPNEYITIGGHL
                     DSWDVSEGAHDDGAGVVQTIEILRAFKALGYQPKHTIRFVLFANEENGTKGGLKYAEK
                     AKENKEQHIFALESDAGGFTPRGFLFTMDKEKLAKVKLWLPLLKPYGTEALDAGGGGA
                     DIGPLNKTFNTPIAGFMPDTQRYFDVHHSVNDIFENVNKRELLLGAVNMAALIYLIDQ
                     HSL"
     gene            9343..9741
                     /locus_tag="PLU36_02370"
     CDS             9343..9741
                     /locus_tag="PLU36_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45626.1"
                     /translation="MNMLKKKFVKIFLLVFILILIIFGYFRFFYVFAEGTKAGVLNTF
                     QKKGYVFKTWEGIIIQSGFKQNVQSNEFEFSVINEKIAKELEKHSGKEVNLHYNRYFG
                     TLPWRGVSVYVVDSIFEIRNPDNKTNITPQ"
     gene            9827..10264
                     /locus_tag="PLU36_02375"
     CDS             9827..10264
                     /locus_tag="PLU36_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45627.1"
                     /translation="MKTLEYNIQLNQPSEKVWDCLWQIDNYKKWTRPFSEGTYYKTSD
                     FKQGNKIHFLLPDGNGMYSTIEVLIINKLVIFNHIGEIKNFEEQPLDEKTKQWTNAKE
                     SYELVETTYGTNLNIKVDCADEYIEYMNTAFLLAIEEIKKLAS"
     gene            10292..10732
                     /locus_tag="PLU36_02380"
     CDS             10292..10732
                     /locus_tag="PLU36_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMZ45628.1"
                     /translation="MATVNVYLTFNGNCEEAFLFYQSVFGGEFPYIGRFNEMPQNDPN
                     CPPMSEEDGKKIMHVTLPISKETCLMGSDTFGDFSPPHIQGNNFSISINTNSTAEADN
                     LFNALSKNGQVVMPLNKTFWGAYFGMFTDKFGINWMVNYDEQTA"
     gene            complement(10932..11156)
                     /locus_tag="PLU36_02385"
     CDS             complement(10932..11156)
                     /locus_tag="PLU36_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ45629.1"
                     /translation="MPIIVNLDVMMAKRKISLNELSEKVDLTLSNLSILKTGKAKAIR
                     FSTLDAICKALNCQPADILEYKSEEENTGN"
     gene            complement(11160..11696)
                     /locus_tag="PLU36_02390"
     CDS             complement(11160..11696)
                     /locus_tag="PLU36_02390"
                     /inference="COORDINATES: protein motif:HMM:NF022636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HMZ45630.1"
                     /translation="MSKTNNIVFWALYAIAWIIFIGLSIEAGGLIVNFLFSLYKPEIV
                     QNLYQKLDLIQMYNENKFAFFSIYSFILIISILKAVLFYTVISLMHTMDRTKPFSTFV
                     AKQISYISYFTLSIGLLSFIGREVTKNLMHEGLALNKVNQFWVDSEAYIFMWAVIHII
                     ATIFKKGVAIQNENDLTV"
     gene            12027..14087
                     /locus_tag="PLU36_02395"
     CDS             12027..14087
                     /locus_tag="PLU36_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HMZ45631.1"
                     /translation="MIVSQLIHPKSIAVIGASDNTTKPGGMVLQNLLKGNFNGPIYAI
                     NPKPLNIESVISYDNINKINTVDLAIIAIPASLCLQTIKELLKKGAKAFIIFSAGFSE
                     AGEQGIAIEKEIVEIINKSNATLIGPNCVGVINQHYKGVFTTPVPEYDPNGCELISSS
                     GATAVFIMESALLTGLRFSNIYSIGNAAQTGVEEILEYLDNTFDEKKSSKVKLLYLEQ
                     VKKPFKFLKHATSLIEKGCRIAAIKSGYSEAGGRAASSHTGALATSDNIIRALFRKAG
                     IVYCSSRDELIAVGSIFQSKKLQGENIAIITHAGGSAVMLTDALTSNGLNVPPFESDR
                     TQELLAKLNPGSSVNNPIDFLATGTAKQLGEIIDFCDNYEAVDAMVVVFGSPGLFNVK
                     DVYELLDEKMNSCKKPIYPVLPSLINAEKEIKQFLNKGRVNFSDEVNLGKALAHVYVS
                     TKYKFEKTILPDMDIVSIRSIINTAQNGYLDAATCVELIEAAGIEIAKQLIFTQAHEV
                     ENIEKLIKFPIVMKVVGPIHKTEVDGVKLNIITTQQAVETFHYLMQIPDATAVLVQEL
                     VKGEELYCGAAKQGDFGHVVLCGLGGIFLELINDTASAMAPMSYSEVRLMVESLRGYP
                     LLKGYRNKKGLNEEKFIDIIQRIGALVHIAPEISELDLNPIKAEDKNMVAVDARICID
                     KINK"
     gene            complement(14090..15004)
                     /gene="moaCB"
                     /locus_tag="PLU36_02400"
     CDS             complement(14090..15004)
                     /gene="moaCB"
                     /locus_tag="PLU36_02400"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HMZ45632.1"
                     /translation="MVDITLKIKSHRIAIAQAVVTVSKQTTIDAINNKTVPKGDVLEA
                     SRVAGLFGIKKTSEMIPDCHPLPIEFAQIKHTIDGLQIFIEVEVHTIYRTGVEVEAMH
                     GASVVALTMYDMLKPIDKGVEINNIKLLKKKGGKSQFGENFSRKLSAAVIVCSDSIAE
                     GKKQDSAGKAIINCLNKYEIDINDYTIIPDEVKDIQDKVNFYCSKQVDIILLTGGTGL
                     SKRDVTPEAIKPLLDREIPGIGEVARSFGQELTPYSMLSRSLGGLKGNTLIIALPGST
                     KGASESMHALFPYILHLFKVMDYFRHEG"
     gene            complement(15010..>15232)
                     /locus_tag="PLU36_02405"
     CDS             complement(15010..>15232)
                     /locus_tag="PLU36_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HMZ45633.1"
                     /translation="DAFKKYPLVCMHIYHSLGIVNAGEISLFVFVSSIHRKEAIKACE
                     EIVERIKKEAPVWGKEIFCNNNSQWKINS"
BASE COUNT         5463 a         2133 c         2474 g         5162 t
ORIGIN      
        1 atacaataaa gatgaaaata atttagaagt attaaaagat tgtataaata aattcattta
       61 gcatttcaaa aaacaactta tttattttta aagaagttag ctttcaaatc ttttactgaa
      121 atttgttctt tgtattgcta aaaaatcaat gaagaaatta ttccataaag aagggttatc
      181 tccactaaaa tataaagaat ttagattatt tattgctttt aggtttttat atataatggc
      241 tttaagaatg gttagcacag ttgttgctta cgaactattt caaattactc aaagctcttt
      301 ttctattggc cttgttggct tatcggagtt tatacctgta ttttcattgg cattatatgc
      361 tggccatgta attgataaaa gcgacaaaag aaccatgctt atgaaaggca tgatgctgta
      421 tgctatttgt gtggttggtt taatagttgt aactcattct tccatgaaaa aacatttatc
      481 tgttactgca atggaaatgt gtttttatac aattattttt tttacaggag ttatcagagc
      541 atttgtaggc ccttcaaaca atgcaattat ttttcagctt gttcctaaaa gcattttgca
      601 ttttgcatct aacttaagtt cctctacttt tttaacagct tctattgtgg gccatgccag
      661 tgcaggcttt tacattgcct ggtttggggt aaatactact tttgtattaa tacttttttt
      721 cgtaatagcc tctgttttca ttgtttcaaa aattattaaa aaaccaatta ccaatacaaa
      781 attggatgtt aaaacctggg atagtatgaa agaaggcttg ttgtacgttt ttaaaaataa
      841 aattttattg ggtgctattt cattagacct ttttgctgta ttatttggtg gggcagttgc
      901 tttattacca gagtttgcaa gcaaaatttt acgtgtaggg ccaataggtt ttggttggtt
      961 aaatgctgct acagacattg gttccatgct tatcattatt tccttaaccc tattcccttt
     1021 aagaaaaagg caaggggaaa taatgctata tgcagtagct tgttttggaa tttgtattat
     1081 gctttttgga ctaagtaatt tttatacagt atcctttatt ttattgcttt tagcaggttg
     1141 ttttgatggt ataagtgtta ttgtacgaag tacaatttta caattaacaa cacccgatga
     1201 aatgagaggc agagtatcct ctgtaaactc tatgtttata aacagcagta acgagctggg
     1261 gcaattagaa agtggtatag catctagact tttaggtctt aggccatcta ttatttttgg
     1321 aggttcggtt actttaattg ttgttattat tacttggttt aaagcaaaac aattaagaaa
     1381 aatgcagtat taaattacaa gtaaattttt aattattttt acccggttta caattttaat
     1441 atgacgttac aacaaaacaa taaagcagtt gccacttggt tgctagtggg tgctgccatg
     1501 ataattattc aggttttatt aggtggctta acccgtttaa cagactctgg tttatctata
     1561 accgaatggc agccattgct tggttcaatt cctccattaa ataatacaga ttggcaaatt
     1621 gctttcaata aatataaaga gctagcacag tttaaattct tacacagcca tttcaattta
     1681 aacgatttta aatttattta cttctgggaa tggtttcata gaatttgggc aagattaatg
     1741 ggtgtagttt ttatccttcc atttgtttac tttatttata aaaaatggat taacagaact
     1801 atgttaatgc ctatgattat tttattttta ttgggcggtt tacagggttt tgtaggctgg
     1861 tttatggtaa aaagtggtgt aggtacaagt ttagtttttg tagatcatac taggcttgca
     1921 atacatttta ttgcagcatt ggttttattg gtttatgtag tttggtttgc actaaaatta
     1981 attattaata acaaccaact tattaatgca ccaaaaataa aacggcttaa tattttaata
     2041 attgctttac ttaccataca attaatttat ggggcattta tggcaggctc tagagctgct
     2101 tatagtgcaa taacctggcc aagcattaat ggctttttct atccaccagg catgttacat
     2161 aaagaaagtt tatggtacga tttgttcaac aatttattgg caatacaatt tatacatcgt
     2221 ggtttggcat atttactttt aattgtaatt attattttta cgcttcaact aaaaaaaata
     2281 aatagtagca agtttttaaa ttcaataaag tttttaccat tttatcttgt aacagtacag
     2341 gttattttag gaatttttac cataataaat tatactacca aatacaaatt aattttaagc
     2401 atcactcatc aatttattgg tatgctatta ttggtaagtt tattttttac cctttttatt
     2461 gttagaaaaa aataaacaaa tattaaattg aaactacctt aaagtgatat attaatttag
     2521 atttgagttt tatacgttta tcttcgtttt atgaatttat attattgtta tgatgcttat
     2581 tgtggttggt gttatggctt tagtcctata ataaaaaaaa ttgcagaaga atataaaaat
     2641 gttttacata cagaggtatt aagtggtgga atgattttac ctgaaacacc aacccatatt
     2701 ggcactatgg ctaattatat tttaaaagca tataaaactg tagaagaata tgcaggaata
     2761 aagtttggaa aagatttttt atggcatata gaaaatccga cacttagcga ttggtttcct
     2821 gatagtttaa aacctgccat tgctctttgt atttttaaag aataccatgc taatttacaa
     2881 attcaatttg ccagcgattt gcagtatgca ttaaattttg aaggaagaga tttaaccgac
     2941 gatgctgctt atttacattt gctaacaaaa tacaatattc ccaaaaatgc attttatgaa
     3001 aaaatgaata gtgaagaata tatagacaaa gccaaatacg aattttcttt atgcaaacaa
     3061 ttgcaagtaa ccggctaccc ttgcgtttta ttacaagcca atgaatcaaa attttattta
     3121 ttagctaaag gttttacaac ctatgaggaa ttaaaaaaaa gagtagaaaa tgttttaaat
     3181 gaacttactg ccaactaatt attatggata aaaatataga aactattcaa aagttttata
     3241 ctgcctttca gcaaaaagat tatgaaacta tgaatagttg ttatgcaaat gatattgctt
     3301 ttagcgaccc tatttttggc ttattacatg gcaaccaggt aaaagcaatg tggcaaatgc
     3361 tttgcaccaa tgctaaagag tttaaactac aatttaataa tattaaaacc gatgatgatg
     3421 aatattatac ttgtagttgg attgctatgt atagattttc tgcaaccaac cgtagagttg
     3481 taaataaatg tactgcatac atgcgattaa aagatggagt aattatagaa cacagcgatg
     3541 gttttaatta ttataaatgg tgcaggcagg cttttggcat taaaggtata ttattaggct
     3601 ggagcggatt tatgcacaaa aaagtttcga ataaagcaag aaaacaatta tttaaatata
     3661 tggagcaaca tggtttataa ttttataaaa gtttaaatac cttttccata atagttttgc
     3721 atatattgca cccataattt ttaaaagcat gaaagaacat aagagtcata ctattgatac
     3781 tgcatcttca acaaaaaaaa taactagttt atttggaaaa gagaactata ttttgatggc
     3841 agttggtgct ataataatta cagttggcat gttattaatg agtggtggca aaaatcaaga
     3901 tccaaatgtt tttgatgaaa gtgttgttta ctcaacaacc agaattaccg ttgcaccaat
     3961 acttatttta attggctttg tagtagaaat ttatgctata tttaaaaaac ccaaagcatc
     4021 tgcttaaata acagatatat atacaaattt ttactagcga tacaatgttt tgtatcgctt
     4081 tttattttta ttcattttaa gcaaggctta tcttcgttta gttatgactt ttaatgatgt
     4141 aattggcaac aaaaatgtaa aacagcattt attagaatta atgctacaaa acaggctacc
     4201 acatgcacta ctttttttag gtaaagaagg tagcggtgca ttacctttgg ctttggcctt
     4261 tgcacaatta attatttgtc agccaaaatc atcgcctgtt gtagaagatt tatttggagg
     4321 catgagtgcc ataagtaacg aaccatcttt ccttcatcca aacgatattg aaagctcaac
     4381 cagttattca aaagctaatt tattagttca ccccgattta catttttctt tcccttttat
     4441 taacaaaaaa aattcagact cttctactat tagtgcagat tatattacag aatggagaga
     4501 gtttttaatg caatctcctt atggtaattt gtacgattgg cttcaagtta taaaagcaga
     4561 aaacagacaa ggtaatattt cagcaaaaga gtgtcttgaa attattaaaa aactaagtct
     4621 aaaaagcttt gaatccgatt acaaagtttt attaatgtgg ttaccagaat tattatctaa
     4681 agagggtaat aaacttttaa aattaattga agaaccacca cccaatacca tttttatttt
     4741 tgttgcagaa aatgaagaaa aaattttacc aaccatttta agcagatgcc aattaataaa
     4801 aattcctttg ccagaaaatg acgaagtaga agaagccctt ttaaaaaagg caaatataga
     4861 acccgaacag gcaaaacaaa ttgcagctat tagtaatggt aattatagag aagctttaca
     4921 tttaatacaa catgcagagg aagactggca aggtttatta agagattggc ttaatactat
     4981 aatgagaaga ttgctgcccg aaaatataaa atggattgaa gaaataagca aaataggcag
     5041 agaaaaacaa aagcaattat tacaatattt taatcatact attgaacaag cattaaggct
     5101 aaaaaccatt ggagctgata atattagctt atctgaaagt gaaaaagatt ttgctcaacg
     5161 ttttaataaa attgccaaca ttagccaaat gcaagctata tcagaagagt tagacaaagc
     5221 aactttttca attgaaagaa acgtaaatgg caaaatagtt tttactgctc tttctgttaa
     5281 attatatcat attctaataa ataaagtagt ggtagaagta taaaaacact taagtattta
     5341 tatatcatta aattactgta taaataaatg aatattggta aaaatataat gtacaaaaga
     5401 aaaaaaattt aacttaaaaa attcaaacct tacttttgct caatccttct acaaaaatga
     5461 gcaacacatt acagagcaac gaatctatct cctcttcaag aaaaaaaatt attgtacttg
     5521 gcagtggccc taatagaatt ggccaaggaa ttgaatttga ttactgctgt gtacatggtt
     5581 tattagccat taaagaatgt ggctacgaag ctataatgat aaactgcaac cccgaaactg
     5641 tgagtacaga ttttgacatg gcagataagt tatactttga gcctgtttat tgggagcatt
     5701 tatgggaaat tatagaatta gaaaaacccg aaggagtaat tgtacaactt ggtgggcaaa
     5761 ctgctttaaa gcttgcaaaa aaattacacg aaaaaggaat taaaattata ggcacttcct
     5821 ataatgatat ggatattgct gaagacagag gacgttttag cgatttgctt aaagagcttg
     5881 aaattcctta tcctaattat ggtacagcct atactgcaga agaagctata gaagttgcca
     5941 ataaagtagg ctatcctgtt ttagttcgcc caagttatgt actaggtggg caaagaatga
     6001 gaattgtaat taatgatgag gaagtagaaa aatcttgtat cagtatttta aaacatatcc
     6061 ctgacaataa aattttaatt gatcattttt tagatcgttg tcaagaggca gaagtagatg
     6121 ctatatatga tggcaaaagt tttcatgtaa tggggattat ggaacatata gaaccagcag
     6181 gtattcatag tggtgatagc aatgccgttt taccagcatt taatttaact ccactagtta
     6241 ttgaaaccat gcagttctat agcgaaaaaa tagcaaaagc attaaatatt aaaggcctta
     6301 tcaatattca gtttgcaatt aaagatggta aagtttatgt tatcgaagcc aacccaagag
     6361 cttctcgtac aacaccattt attgcaaaag cctaccaaat accatactta aatattgcca
     6421 ccaaagtaat gctagataaa gcaaaactta gcgactttaa tttagaaaaa aaattagatg
     6481 gctacgctat taaggagcct gtttttagct ttaataaatt tccaggtgtt aacaaagaac
     6541 taggccccga aatgaaaagc actggagaag caatcaggtt tattaaagat ttgagagacc
     6601 cctactttag aactttgtat aaagaaagaa gcatgcactt aagtaaataa tcttcttctt
     6661 aaaacaataa ttgtacttat ctctttatct tttttgattt aatttcttta aaaaaaagac
     6721 cacattggct ttaatatgcc taaaaacaat aactttagtt taaattttta taatgaggtt
     6781 attgttgatg cttgccatat taattgtttt ttttacaact gtaactgctc aaaaagtttt
     6841 taaagatgca aatggcagct atgggttaaa ggataataat ggaaaaataa tattaaaacc
     6901 tacttatacc ttcattcgtt cttttgtaga tggctttgca gccattgcca ttaacaagta
     6961 ccccaatatt aaatggagtt ttataaatac taaaggccaa ataattactc cttttaagta
     7021 tgataaaatt gatttttttt cggaaggatt ggctgttgtt aaacttaaca aattgtatag
     7081 ttacataaat agcttaggta aagaagtatt tggatttatt tttttagaag catttggatt
     7141 tcatcaaaac attgctgttg taaaaaataa aaacggaaaa tatggagtaa ttaataaaca
     7201 aggtaaaaca gtggttaatt ataagtacga taatatgaat agccatttta ccgaaggttt
     7261 agcatgggta accttaaata ataaagtagg ttttattaat gcaaaaggta tagaagttgc
     7321 tgcttgtatt tatgatgatg caggtaattt taatgaaggc atggctcctg taaaacaaaa
     7381 tggcaaatgg ggttttatta ataataaagg caaagtagta ataccttttc aatacgatgt
     7441 tacctcttta aactctttta ataatggatt agtaaaagta aaaattaatg aatactacgg
     7501 gtttattaat aaacaaggag aagaagttat accttttata tacgaaaacg tagatgactt
     7561 tagcgaaggt cttgcaggct ttttaaaaga agggaaatat ggatttatgg atttaacagg
     7621 taaagaaata ataaaaccaa tttataaata tacctggggt tttaataatg gccttgcagc
     7681 agttgaagtt gaaaatgaaa aaaaagaaaa caaatggggc tttatcaata aaaaaggaat
     7741 cctggtaatt ccggctctgt actcttctat tctgccacag caaaacaaac acacccataa
     7801 gataattttt gggttttata aaaacaaagc ctacgtttct ttatcagata aaaacaactt
     7861 ttacattaat aataaaggct taaaaattag cgattaagtt gtatcccttc cttagtctaa
     7921 atttctcatc aaattaattc taaagtgtac tatccaatta atatctttag aagtatgaaa
     7981 aaaatattat tactgctgct tctgccaatt gtagccaata gtcagcaaac cgattcttta
     8041 tttattaaaa aaatagctga tgaaatttta actaatggca aagcttatga cttattatac
     8101 cacttaacaa aaaaaattgg aggcaggctt gcaggttcgc ctcaatacaa caaagcagta
     8161 aattgggggt ttaacactat gcaacaaatg ggtgcagata aggtttttct gcaagagtgt
     8221 attgtaccac attgggtaag gggtgttggc gattttgcaa cattacaaaa tactaattcc
     8281 tcttctgtca aaaaacaatt aaatattacc gctattggca actctttagg taatactaaa
     8341 atcatcaaag ctcctattat tttaatccac aattttcaag aattagaaaa taaaaaagag
     8401 gaagtaaaag gcaaaattgt tttttataat gctgcattta acccaaccaa tataagaaca
     8461 tttaaaagtt atggagaaac ggcatcctac cgtgtaaatg gtgctagccg tgctgcaaaa
     8521 tatggtgcag ttgctgtaat tgtaagaagt ttaaccgaag caacagataa tcatcctcat
     8581 acgggaacat tacgttataa cgattctttt cctaaaatac cagcagtatg tgtagggtta
     8641 aaagatgcag attacatagc acaaaatata aaaaaaggta attggcaagt agccctaaaa
     8701 acaaatggaa aattattgcc agatactatt aatcataatg taattgcaga actaaaagga
     8761 acagaatttc ctaatgaata tattactatt ggtggacatt tagatagttg ggatgtaagc
     8821 gaaggggcac atgacgatgg tgcaggtgtt gtgcaaacta tagaaatatt aagagcattt
     8881 aaagctttag gctaccaacc caaacatact ataagatttg tattatttgc taacgaagaa
     8941 aatggaacaa agggcggttt gaaatatgcc gaaaaagcta aagaaaataa ggaacaacat
     9001 atttttgctt tagaaagtga tgcaggtggt tttacaccaa gaggtttttt atttacgatg
     9061 gataaagaaa aattagcaaa agttaaattg tggttgcctt tattaaaacc ttatggtacc
     9121 gaagctttag atgctggtgg aggtggtgca gatattggcc ctttaaacaa aacatttaac
     9181 accccaattg ctggctttat gccagatacc caaagatatt ttgatgtgca tcattccgta
     9241 aatgatattt ttgagaacgt aaataaaaga gaacttttat taggtgctgt taatatggct
     9301 gcactaattt atttgattga tcaacatagc ttataaaaca caatgaatat gcttaaaaaa
     9361 aagtttgtaa aaatttttct gcttgtattt attttaatac taatcatttt tggatacttt
     9421 agattttttt atgtatttgc cgaaggcact aaagccggtg tattaaatac ttttcaaaaa
     9481 aagggatatg tatttaaaac ttgggaaggc ataattatac aaagcggctt taaacaaaat
     9541 gtacaaagca acgaatttga gtttagcgta attaacgaaa aaattgcaaa agaattagaa
     9601 aaacattctg gcaaagaagt gaatctacat tacaatagat attttggaac attgccatgg
     9661 cgtggtgtta gtgtatatgt agtggatagt atatttgaaa ttagaaaccc agacaataaa
     9721 actaatatta caccacaata aactttagta gaaaaaacta ccaactattt tttaatagca
     9781 ataaaaaaaa atttactatt tttaataata tttaatagta gaaattttga aaacattaga
     9841 atataatata cagcttaacc aaccttctga aaaagtttgg gattgtcttt ggcaaataga
     9901 taattataaa aaatggacaa ggcctttttc cgaaggaacc tactacaaaa cttctgattt
     9961 taaacaaggc aacaaaattc atttcctctt acccgatgga aatggtatgt acagtacaat
    10021 tgaagtttta ataataaaca aactggttat ttttaatcat ataggagaga taaaaaattt
    10081 tgaagagcaa ccattagatg aaaaaacaaa acagtggaca aacgctaaag aaagttatga
    10141 gctagtagaa actacatacg gaactaatct taatataaag gtagattgtg cagatgagta
    10201 catagaatat atgaacactg cttttttatt agctattgaa gaaataaaaa aattagctag
    10261 ttaatattta ttaataataa aaaatataaa aatggcaaca gtaaatgttt acttaacttt
    10321 taatggtaat tgtgaggaag cttttttatt ctatcaatct gtttttggtg gtgagtttcc
    10381 ttatatagga cgttttaatg aaatgccaca aaacgatcct aattgcccac caatgagtga
    10441 agaagatgga aaaaaaatca tgcatgttac ccttcctata agtaaagaaa cctgtttaat
    10501 gggcagcgat acatttggcg atttttctcc accacatatt cagggtaata atttttccat
    10561 ttcaataaat acaaacagca ctgccgaggc tgataattta tttaatgcac tatctaaaaa
    10621 tggccaagta gtaatgccac taaataaaac attttgggga gcctactttg gtatgtttac
    10681 agataagttt ggaataaatt ggatggtaaa ttatgatgag caaacagcat agtaaacatg
    10741 agtagctcaa ctataaaatt ttattgcatt ttttgccttc tattctttat tgctttgcat
    10801 taacatgtat cattaatgtt ggcttaccac aacaaggtaa ataggtagca attgcaattt
    10861 taatatttca ttgtagaaat attaattaat agctcattac aatctactat tgtttttgta
    10921 ttaccatatt atcagttacc tgtattttcc tcttcacttt tatattctag aatatctgca
    10981 ggctggcagt tcaaagcttt acaaatagca tccaatgtgc taaagcgaat tgcttttgcc
    11041 ttgccagttt ttaagataga aaggttagat aatgtcaaat caactttttc tgaaagttca
    11101 ttcagagaaa ttttacgctt tgccatcatc acatctaagt ttacaatgat tggcataatt
    11161 tatacagtta aatcgttttc gttttgaata gctactccct ttttgaaaat tgtagcaatg
    11221 atatgaatta cagcccacat aaaaatatag gcttcgctat caacccaaaa ttggttaacc
    11281 ttgttaagag ccaagccttc atgcattaaa tttttagtta cttctctgcc aataaaactt
    11341 aatagcccta tggaaagggt aaaataactt atgtacgaaa tttgtttagc aacaaaagtg
    11401 ctaaacggtt tggttctgtc cattgtatgc attaagctta ttacggtgta aaataaaaca
    11461 gcttttaaaa tggaaataat tagaataaaa ctgtaaatac taaaaaaagc aaacttgttt
    11521 tcattataca tttgaattaa gtctaacttt tggtagagat tttgaacaat ttctggctta
    11581 tacaaactga ataagaagtt tactattaag cctcctgctt caatagataa gcctataaaa
    11641 attatccaag ctatagcata caaggcccaa aatacgatgt tgtttgtttt tgacatattt
    11701 aatatttttt ttcttttaca ttgcaaacat aataaatatt tattgataaa caataaattt
    11761 atattttaat tcaaaaaata ttttacatta aaaaatttat tatttcttag ggtttacatc
    11821 agattattaa ttatgcattg tattaaaaat acaaaagcaa ctataattgg tgcccaatta
    11881 gttagagaaa taagtgttga gtaatattga ttgcttgcat gttttgttac ttagaaacat
    11941 agagctaact ttcaaagcca tgactcaggt catttaggta tgaggttttc aaatttatat
    12001 ttgtatttaa aacatttata tagagtatga ttgtttctca attaattcac cccaaaagta
    12061 tagctgttat tggtgcatca gataatacca ctaaaccagg tggtatggtt ttacaaaact
    12121 tgcttaaggg taattttaat ggtccaattt atgccattaa tccaaagcct ttaaatatag
    12181 aaagtgttat aagctacgat aatataaata aaataaatac agttgattta gccattattg
    12241 caatacctgc aagtttgtgt ttgcaaacaa ttaaagagtt acttaaaaaa ggagcaaaag
    12301 catttattat tttttctgca ggtttttcag aagcaggaga gcagggcata gccattgaaa
    12361 aagaaattgt agaaataatt aataaatcca atgcaacatt aattgggcct aattgtgttg
    12421 gtgtaattaa ccaacattat aaaggtgtat ttacaacacc agttcctgaa tatgacccta
    12481 atggttgcga gttaatttca agctctggag caacagcagt ttttataatg gagtctgctt
    12541 tgttaacagg gttaaggttt tctaatattt attccattgg caatgcagca caaacaggtg
    12601 tagaagaaat tttagaatat ttagataata cttttgatga aaagaaaagt tctaaagtta
    12661 aattattgta tttagagcag gtaaaaaagc ctttcaaatt tttaaaacat gctacttcat
    12721 taatagaaaa aggatgcagg attgctgcta taaaatctgg ttattcagaa gctggcggaa
    12781 gagctgcctc ttctcatact ggtgcattgg caacatctga taatattatt agagctttat
    12841 ttagaaaagc aggtattgtt tattgtagca gtagagatga gttgattgct gttggcagta
    12901 tttttcaatc taaaaaacta caaggcgaaa atattgccat aataacccat gcaggaggct
    12961 ctgcagttat gcttacagat gctttaacat ctaatggttt aaacgtacct ccttttgaaa
    13021 gtgatagaac acaagaactt ttggcaaaat taaatcctgg ttcttcagta aataatccaa
    13081 tagatttttt agcaacaggc actgcaaaac aattaggaga aataatagat ttttgcgata
    13141 actacgaagc agtagatgca atggttgtgg tttttggaag ccccggttta tttaatgtaa
    13201 aagatgtgta tgaattgctt gatgaaaaaa tgaatagttg caaaaaacct atttatcctg
    13261 ttttaccatc tttaataaat gccgaaaaag aaataaaaca atttttgaat aagggaagag
    13321 taaatttttc ggatgaagta aatttaggca aagccttggc tcatgtatat gtttctacaa
    13381 aatataagtt cgaaaaaaca atattgcccg atatggatat tgtttctatt aggtctatta
    13441 ttaatactgc tcaaaatggt tatttagatg ctgctacatg tgtagaatta atagaagcag
    13501 caggtattga aattgcaaag caactaattt ttactcaagc tcatgaagtt gaaaatattg
    13561 aaaaattaat taagtttcct attgtaatga aagtagtagg gccaattcat aaaacagaag
    13621 tagatggagt aaaattgaat attattacca cacaacaagc tgtagaaact ttccattatt
    13681 taatgcaaat acccgatgct actgcagtac ttgtacaaga gttggttaaa ggagaagagt
    13741 tgtactgtgg tgcagcaaaa caaggcgatt ttggacatgt ggttttatgt ggcttaggtg
    13801 gaattttttt agaactaatt aatgatactg catctgcaat ggctccaatg agttatagcg
    13861 aagttaggtt aatggttgaa tcattaagag gttatccatt acttaaagga tatagaaata
    13921 aaaaaggcct aaacgaagaa aagtttattg atattattca aagaattggt gcattagtac
    13981 atattgcacc agaaataagt gagttggatt taaatccaat taaagccgaa gataaaaata
    14041 tggttgcagt agatgcaaga atatgtattg ataaaataaa taaataacat tacccttcat
    14101 gcctaaaata atccattact ttaaataagt gtagtatata aggaaataaa gcatgcatac
    14161 tttctgatgc accttttgtt gaacctggta aagctattat caatgtattt ccttttaatc
    14221 ctcctaaact tctggacaac attgagtaag gtgtaagctc ctggccaaaa cttctggcaa
    14281 cttctccaat accaggtatt tctctatcta acaagggttt tattgcttct ggtgttacat
    14341 ctcttttaga taaacctgta ccacctgtta ataaaataat atctacttgt ttgctacaat
    14401 aaaaatttac tttatcctga atatctttta cctcgtctgg tataattgta taatcattaa
    14461 tatctatttc atatttattt aaacaattta tgattgcttt acctgcagag tcttgctttt
    14521 taccctcagc tatactatca gaacaaacaa ttactgctgc agaaagtttt ctcgaaaaat
    14581 tttctccgaa ttgagattta cctcctttct ttttcagtaa tttaatattg tttatttcaa
    14641 ctcctttatc aattggcttt aacatgtcat acatggttaa tgctactaca gatgctccat
    14701 gcatagcttc tacttctaca ccagttctat aaattgtatg cacttctacc tcaataaaaa
    14761 tttgcaaccc atcaatagta tgttttattt gagcaaactc tatgggtaaa ggatgacagt
    14821 caggaatcat ttcactagtt ttctttattc caaataaacc agcaaccctg cttgcttcta
    14881 aaacatctcc tttgggtaca gttttattat taatagcatc tatggtagtt tgtttactaa
    14941 cagttacaac tgcttgagct attgcaatac gatgcgattt tatttttaac gttatatcta
    15001 ccattgttat taagaattaa ttttccattg tgagttattg ttacaaaaaa tttccttgcc
    15061 ccacacaggt gcttcttttt ttattcgttc tacaatttct tcacaagctt taatggcttc
    15121 ttttctatgt atagaagaaa caaaaacaaa taaactaatt tctcctgcat ttacaatacc
    15181 caggctatga taaatgtgca tgcaaaccag tgggtatttt ttaaaagcat ct
//
LOCUS       DAOQPK010000027        15018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_2022_length_15018_cov_4.298269, whole genome shotgun sequence.
ACCESSION   DAOQPK010000027 DAOQPK010000000
VERSION     DAOQPK010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 15018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15018
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2022_length_15018_cov_4.298269"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="PLU36_02410"
     CDS             complement(<1..948)
                     /locus_tag="PLU36_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45634.1"
                     /translation="MIKKTLAACILTIAFCLISKHIFAQPDSILKVYATDFQEERIFI
                     HFDKTTYLPGETIWFKAYLASGIDLSYTSKNFYAEFTDSAGNVLQHITAPIVLSSAKA
                     MFVIPKDYASNNIFLRAYTSWMLNFDSSFIYKKNIPVIQPVNTKKFRAQKPKTSITFF
                     PEGGNLVNNIKSVVAFKANTKTGKPVKIKGLLFNNKGKILDSISSVHDGMGSFTLTPI
                     PNETYYVQWMDEWNTKYKSILPTANEEGIVMNVVQQEYDKLNVQLHRQENATNEFKQL
                     RVVATMHKNMVYRSNVKLVNNTNITVPIPIAHLPTGVLQI"
     gene            complement(976..2001)
                     /locus_tag="PLU36_02415"
     CDS             complement(976..2001)
                     /locus_tag="PLU36_02415"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ45635.1"
                     /translation="MKKIVLAVLLIFQIATVVVAQPNQTAKELHETARTFMRQGDYDN
                     ALLVIKRAAEQDPDNLEILKDLAFLYFLKRDYANAMQIGKVIIDRPDADVQSFQILGL
                     TYKAIAEYKECKKLYKKALAKFPESGVIYNEYGELLAMDNDLNNAIVQWEKGIEVDAN
                     YSSNYYNAVKYYAQNPNPELLWIALHGENFVNLESYTARTKEIKKIILEAYKKMYAYS
                     NLLENTPKKTSGFETDVLSILNNSKDIANNGINAESLMAIKARFVLDWFEGKQQKFPF
                     RLFDHYQHMLKEGIFDAYNQWLYGSVANADAYELWIKNNDKQAEAFKKFQNARVYKIP
                     QGQYYKK"
     gene            complement(2144..3382)
                     /locus_tag="PLU36_02420"
     CDS             complement(2144..3382)
                     /locus_tag="PLU36_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HMZ45636.1"
                     /translation="MAIIKPFKALRPQPQLAKQVASRPYDVLNSEEAKQEALGNPYSF
                     LTVTKSEITLNSNIDIHSQEVYDKAKENIETFIKHNVLFKEDKSCYYIYQLVMDGRSQ
                     TGLVVASSIDDYENGLIKKHEFTRPEKELDRINHIKTTGAQTGNVFLAYRNNVNIDTI
                     VENWKASKKPVYDFVADDQIQHSIWVVNDDNVIQNISSIFEKEIPCTYIADGHHRAAS
                     ASKVRKDTGIKHTDGGDYFLTTLFPSNQLQIMDYNRVVKDLNGLTLEQFFDLLKNNFE
                     IILTEKNQVKPSTLHEFGMYFENNWYKLIAKNGVYTNDPIGVLDVTILSNLILDAILG
                     IKDQRTDKRVDFVGGIRGLKELEKRVDSGEMKVAFSLYPVTINQLFDIADCNNVMPPK
                     STWFEPKLRDGLLTHLIYEA"
     gene            complement(3412..4482)
                     /gene="serC"
                     /locus_tag="PLU36_02425"
     CDS             complement(3412..4482)
                     /gene="serC"
                     /locus_tag="PLU36_02425"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HMZ45637.1"
                     /translation="MRKHNFNSGPSVLPKEVLEQASEAIINFNGINLSLLEIGHRTNW
                     FVDVIEESKLLVKELMQLNNNYEVLFLHGGATTQFMQVPLNLLNENETAAYCDNGIWG
                     SKAISEAKIFGKVNVIASSKDQNYNYIPKSFTLPDNTKYLHLTSNNTVEGTQWHQFPE
                     TTIPIVADMSSDIFSRQLNFNQFQLIYAGAQKNMGAAGVSLVVIDKNILGKVSRQISP
                     ILDYRKHIEAASLLNTPPVFAIYVALLTLRWIKKEGGLAEMENRANKKSSLLYNTIDS
                     LPLFECAIKKEDRSKMNAVFCIKEEVLQNKFLEECKEEGMIGVKGYRTVGGLRVSMYN
                     ALSLESVEAITMLMKNFANKYS"
     gene            4639..5139
                     /locus_tag="PLU36_02430"
     CDS             4639..5139
                     /locus_tag="PLU36_02430"
                     /inference="COORDINATES: protein motif:HMM:NF025734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HMZ45638.1"
                     /translation="MAKKIFYSFLAIIAVLQLFQPDRNISTENFKVELANHYSVPDSI
                     ESLLNTVCYDCHSNNTDYPWFVNIQPIGWYLQGKINKGKSKLNFSEFGSLFKEKAIEK
                     LENIEEVMKKNTMPLTSYKWYNKNADLTIEQREMISKWAINLSKQIASDSTAALNADS
                     LPVKPL"
     gene            5239..5595
                     /locus_tag="PLU36_02435"
     CDS             5239..5595
                     /locus_tag="PLU36_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45639.1"
                     /translation="MVNYINDSTNQLARYKITAECTEPFEHLIIAGFCSNVQTENILY
                     RHLQNVCKQSKGNFSNAHTLINDTITHWQNTFNLPAVQFLKNINTEKTACRYNKICLY
                     KINHQATEELLYNIYL"
     gene            complement(5617..6546)
                     /locus_tag="PLU36_02440"
     CDS             complement(5617..6546)
                     /locus_tag="PLU36_02440"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HMZ45640.1"
                     /translation="MTKRIFQTLVIFLSSTILVYAQKTEEQNDKKKDKESSVTKKEKE
                     EKYVLVIDGDQITVNGIPVEKYALDNVEIVRGKRFKGQENGKHFFENFRELNELFIDK
                     MKPNKAILGVSTKFEKEGAIVVNVSKESAAEKAGLQIEDIITQIDKEIVSDTNSLYKI
                     IGKYKPNDKVTITYIRNGKTQTTTAILDKYKGPNVVSLNNIYEGFDMKNNGGNFSPRR
                     PKIGLEIQDVEEGNGVKVLDVEDESPAEKAGLKEEDIITEINGKELKSVDDFKVNLKD
                     LKEGDTFKLKYKRSNKTIQTEIKLPKRLKKASL"
     gene            complement(6638..7507)
                     /locus_tag="PLU36_02445"
     CDS             complement(6638..7507)
                     /locus_tag="PLU36_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HMZ45641.1"
                     /translation="MNPKNVLGNPTKLLLLFFSIFILSCINNNANNIPVLTSKNDSAK
                     LKESIKVIVDSNAIKFERKPVNYDSNKTYIYLTFDDGPGHGTMNCLDVCKKEGIKASF
                     FMVGKHANDSWGQQTVKTIRNAYPQFLLSNHSYTHTNEKYVYFYSHPLMAEQDFYKAQ
                     ESLNVPYKIIRLPGNNAWVTKDKIRTSKLVKPVCQKLNDAGFNVIGWDVEWNFTKGNS
                     FPVQSPQQVAAQVKNAASGQSFTKKHIVILTHDRMFRTPEYANWLSEFIKTLKQNSNF
                     VFETIDNYPNLKF"
     gene            7689..8294
                     /locus_tag="PLU36_02450"
     CDS             7689..8294
                     /locus_tag="PLU36_02450"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HMZ45642.1"
                     /translation="MSFTLASLPYGYDALEPHIDTTTMQIHHGKHHQAYVDNLNKAIA
                     GTDYEQKTLDQINAIAGSISAAVRNNGGGHWNHSFFWESLAPNSGGLPSGNLADAINT
                     AFGSFDEFKAKFANAGATRFGSGWAWLIVKEGKLEICSTPNQDNPLMDIAETKGTPIL
                     GVDVWEHAYYLKYQNKRADYLSAIWNVVNWNKVAERYNAAK"
     gene            complement(8363..11002)
                     /locus_tag="PLU36_02455"
     CDS             complement(8363..11002)
                     /locus_tag="PLU36_02455"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMZ45643.1"
                     /translation="MKKFLFLLITLFTTFFSYAEHISGGELFYTYIGPGAGNTDRYLV
                     TMRLFRECGPTGPNFAALNGENVTIGVYNNPGLTLVTTQKLTQQFSGTPPEVHNTAGA
                     NPCLLPFVSVCYQVGIFQANIDLPKTENGYTLSWIRYTRTSITNVSNSSSQMGATFTT
                     KIPGTKQLPVGNNNCPTFAVRDTNTVCISTDFLLDFSASDTDGDSLAYKLSAAYDGQD
                     GPNPSPNPPPPSVLNPVSLNYPAPFSSNNPFGTGASINPKTGIISGKAPPIAGKYVVC
                     VTVEEWRNDTLINEHRKDFILRVANCSSVKPNAGPDDRTCDGFTYFFENQSPNPAIIS
                     YVWSFGDGNTSTVPTPTYTYSDTGKYLIKLKVTATGGCQDSSYKYIYAFPGFKPGINT
                     VGTCVVSPINILDATTTVYGTVNSWRWNFGDLTTVADTSNKKDTAWKYPAPGTYNISL
                     IVTNTKGCLDTVNKNVIVKDLPTISLPFKDTLICSIDTLPLIANTTGNISWAPNYNII
                     SPTSLNPSVYPKDTTTYVITVNDNGCISKDSIKVNVLDFISVTAGPDSTVCLTDTFRL
                     STISHALSYVWSASTGEIVPQVKFPLVKPTAITKYYVTANLGKCQDYDTIVITPIPYP
                     KVAINAINSICYGDSITLSTNNFIGSSFYWSPSTFITNANSLSPTVKPTDTTFYIITV
                     NDTLGCPKPFSDSIQVNVIPKVVVFAGNDTVVVKNQPLQLQAVTSPIPNNSTYVWSPS
                     TGINNTTIFNPIATLNINSDSIKYKVTVKRPEGCIGSDELVVKIFKTQPDIFIPTAFT
                     PNNDGKNDILKPIPVGISQIDFFRIYNRFGQLIYETKEYLKGWDGNVNGTPQASGTYV
                     FTAQGVDYTGKVIFKKGTVVLIR"
     gene            complement(11142..11996)
                     /locus_tag="PLU36_02460"
     CDS             complement(11142..11996)
                     /locus_tag="PLU36_02460"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ45644.1"
                     /translation="MQQSIFNYKGHNIAYTIYGSGKPLVLLHGFGEDSTIFNNQVNYL
                     KNNFQVIVPDLPGSGNSSLLKLRSVIEINKIMLHEAAITDYANCIAALLNFLAINECI
                     LLGHSMGGYITLAFAEKFPNYLKAFGLLHSTAFEDNEIKKENRMQSIAMIEEYGAFSF
                     LKNVIPNYFGKKFSMQQAPQINKLIDKSAKFTNEALQQYYFAMMNRPDRTEVLKDASV
                     PVLFIIGTEDVPAPMKDVLQQVHLPQKSYVHIFDEVGHMSMVEATDKLNKSIEVFCKD
                     IFQNLTLN"
     gene            complement(11996..13570)
                     /gene="lnt"
                     /locus_tag="PLU36_02465"
     CDS             complement(11996..13570)
                     /gene="lnt"
                     /locus_tag="PLU36_02465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HMZ45645.1"
                     /translation="MKTFKYLLLSTVAALMMYIAWPPQPITIAVFFAFIPLLFIADTV
                     SNKKLFFAFVFIALLLWNVFTTWWIWNSTSVGSIFAIVANSFVMYLPWWGYYHTKNKF
                     NKQISYLALICFWMLFEYIHLNWELSWPWLSLGNVFANSPYWIQWYEYTGVGGGTLWI
                     LFCNILIFETTKQWSTISAKVKFGYLLAVIFAISFPIIISYQIANNREKLIAKANENV
                     VIIQPNIDPYLKFESGNINSQIEKLVSLTASSIDSNTVLVLWPETALSSAIPEDAISN
                     ATVYKPIFDFLKNHPKITLVSGIETYKIMSSETKYSQKTTQDVYYESYNAAIAINSNK
                     VSNIYIKSKLVPGVETLPSFLKILSPVFERFGGTTGGYAKDETSKVFQINNQLNVAPV
                     ICYESIYGEYLSTYIKKGANLITILTNDGWWGNTAGHKQHLAYAKLRAIETRCWIVRS
                     ANTGISAVINDYGLVLNTLDWNKEGAIKQTVQINNAKSFYVQHGDYLYFIFSIIAFIL
                     IGYFLFILLRKNKKTQ"
     gene            13674..14090
                     /locus_tag="PLU36_02470"
     CDS             13674..14090
                     /locus_tag="PLU36_02470"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HMZ45646.1"
                     /translation="MKKILIFVPGFIWLIISLWLFTLPGTAIPKFDLFTKLQGDKLIH
                     AIIFIVLCISFLLPLHNIQPYCANNKFWFLFIILVFGMYGLAIEFVQEKFIKFRSFDV
                     GDIVADWVGCFIGFKLFIRYWIKKIGLSRNRDRNQN"
     gene            14656..>15018
                     /locus_tag="PLU36_02475"
     CDS             14656..>15018
                     /locus_tag="PLU36_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HMZ45647.1"
                     /translation="MALFNIRVYGILKDDNNNVLLSDEYIYGNYFTKFPGGGLEFGEG
                     TRECLIREFKEETGLDIIVGKHIYTTDFFQISAFNNKDQIISIYYYVHASNLSALKTS
                     ATPFNFKDEQLIDKKGEVE"
BASE COUNT         5017 a         2319 c         2106 g         5576 t
ORIGIN      
        1 tatttgtaaa acccctgttg gtaaatgagc tattggaatt ggaacagtaa tattggtatt
       61 gtttaccaat ttaacattgc ttctgtacac catattttta tgcatggttg caactactct
      121 taattgctta aactcatttg ttgcattttc ttggcgatgt aattgtacat ttagtttatc
      181 atactcttgt tgtaccacat tcattacaat tccttcttca tttgcagtag gtaaaattga
      241 tttgtacttt gtattccatt catccatcca ttgtacatag taagtttcat tgggtattgg
      301 agttaatgta aaactgccca taccatcgtg tacagaggaa attgaatcta aaattttacc
      361 tttattatta aacaataacc cttttatttt tacaggctta cctgtttttg tatttgcttt
      421 aaaggctact acacttttaa tattgtttac taaatttcct ccttcaggaa aaaaagtaat
      481 tgaagttttt ggcttttgtg ctctgaattt ttttgtattt acaggctgaa ttactggaat
      541 atttttttta taaataaaac tgctgtcaaa gtttagcatc cagctagtat atgctcttaa
      601 aaaaatattg ttagaagcat aatctttagg aataacaaac attgcttttg ccgatgataa
      661 aacaattggt gccgtaatat gttgtaaaac atttccagca ctatcagtaa attcagcata
      721 aaaattttta ctggtataac ttaaatcaat accactagct aaataagctt taaaccaaat
      781 agtttcgcct ggcaagtatg tagttttatc aaaatgtata aatattcttt cttcttgaaa
      841 atcggtagca tacactttta aaatagaatc gggctgtgca aatatgtgtt tgcttattaa
      901 acaaaaagca atagttaaaa tgcaggcagc taatgttttt ttaatcatag ctactaatgg
      961 ttaaaatata aagaattatt ttttgtagta ttgaccttgt ggaattttat atactcttgc
     1021 attctgaaat tttttaaatg cttctgcttg tttatcatta ttttttatcc aaagttcata
     1081 agcatctgca ttggcaacag agccataaag ccattgatta tatgcatcaa aaattccttc
     1141 ctttagcatg tgttgataat ggtcaaataa tctaaatgga aatttttgtt gtttgccttc
     1201 aaaccaatct aaaacaaatc ttgctttaat agccattaaa ctttcagcat taattccgtt
     1261 attggctatg tctttactat tgtttaagat agataataca tcagtttcaa atccactggt
     1321 ttttttagga gtattctcta ataagttact ataagcatac atttttttat acgcctctaa
     1381 aataattttt ttaatttctt ttgttctagc agtatagctt tctaggttaa caaagttttc
     1441 tccatgtaaa gcaatccaca gtagttctgg atttgggttt tgtgcataat actttactgc
     1501 attataataa ttactactat aattggcatc tacttcaatt cctttttccc attgaacaat
     1561 ggcattattt aaatcattat ccattgctaa tagttcgcca tattcattat aaattacacc
     1621 actttcagga aattttgcca atgctttttt atataatttt ttacactctt tatattcagc
     1681 aatagccttg taggttaggc caagtatttg aaaagattga acatcagcat ctggtctatc
     1741 aataataact tttcctattt gcattgcatt ggcataatct ctttttaaga agtatagaaa
     1801 tgccaggtct tttaaaattt ctaaattatc tgggtcttgt tcggcagctc tttttataac
     1861 cagtaatgca ttgtcataat ctccttgtcg cataaatgtt ctagctgttt cgtgcaattc
     1921 ctttgcagtt tgatttggtt gagcaaccac taccgttgca atttgaaaaa ttaataaaac
     1981 agctaaaact atttttttca tgtataatat tattttggcc tttggtattg tttagttgaa
     2041 ttacaactta ttatttaatt attaaaatat ttgctctact aaatttttgg ttgaaattta
     2101 tagaggtgag ataatatttt aagaagtata aataaattgt tttttaagcc tcataaatta
     2161 aatgtgttag taacccatct cttaattttg gctcaaacca agtacttttt ggtggcatta
     2221 cattgttgca atctgcaata tcaaataact gatttatagt tacaggatat aaactaaaag
     2281 caactttcat ttcaccacta tctactcttt tttctaactc ttttagcccc cttattccac
     2341 caacaaaatc aacacgttta tcggtgcgtt gatctttaat tcctaaaata gcatctaaaa
     2401 ttaagttaga taaaatggtt acatctaaaa cacctattgg gtcattagta taaactccat
     2461 tttttgctat aagtttatac caattgtttt cgaagtacat accaaattcg tgtaaagttg
     2521 agggctttac ttgatttttt tccgttaaaa ttatttcaaa attatttttt aacaaatcga
     2581 aaaattgttc taaagttaat ccgttcaaat cttttacaac acgattataa tccataattt
     2641 gtaattgatt agaaggaaat aaagtggtta aaaaataatc tcccccatct gtatgtttta
     2701 tacctgtatc ttttcttact ttagaggcag atgcagctct gtgatgccca tctgctatat
     2761 atgtacatgg aatttctttc tcaaaaattg atgaaatatt ttgaataaca ttatcatcat
     2821 ttacaaccca aatagaatgt tgaatttgat catctgcaac aaaatcatac actggctttt
     2881 tagaagcttt ccagttttct acaatagtat ctatattaac attatttcta taagctaaaa
     2941 aaacattccc agtttgggct cctgtagttt taatatggtt tattctatct aattcttttt
     3001 ctggccttgt aaattcatgt ttttttatta gcccattttc ataatcatca atactacttg
     3061 ctaccaccaa acctgtttgg cttctaccat ccataactaa ttgataaatg taataacagc
     3121 ttttatcttc tttaaataaa acattatgtt ttataaatgt ttctatattt tcttttgctt
     3181 tatcatatac ttcttgagag tgtatatcta tattactgtt taaggtaatt tcactttttg
     3241 ttactgttaa aaacgaataa ggattaccta aagcttcttg ctttgcttct tcactattca
     3301 acacatcgta tggacggcta gctacttgtt ttgctaattg tggttgtggt cttaaagctt
     3361 taaatggttt aataatagcc atgttctgat tttttattta ttgaatttat tttatgaata
     3421 tttatttgcg aaatttttca ttaacattgt tattgcttct acactttcaa gtgatagtgc
     3481 attatacatg cttactctta atcctcctac tgttctatac ccttttactc caatcattcc
     3541 ttcttcttta cattcttcta aaaatttatt ttgtaaaact tcctctttta tacaaaatac
     3601 agcattcatt ttactcctat cttctttttt aattgcacat tcaaataatg gtaaactatc
     3661 aatggtattg tataataaac ttgatttttt gtttgctcta ttttccattt ctgctaatcc
     3721 gccttctttt ttaatccaac gcaatgtaag caaagcaaca taaatagcaa ataccggggg
     3781 agtatttaat aaacttgcag cttctatatg ttttctataa tctaaaatag gagaaatttg
     3841 gcgacttact tttcctaaaa tatttttatc tattactact aaactaacac cagcagcacc
     3901 catatttttt tgtgcaccag cataaattaa ttgaaactga ttaaaattta attgcctact
     3961 gaaaatatcg ctgctcatat cagcaacaat aggtatggta gtttcaggaa actggtgcca
     4021 ttgtgttcct tcaacggtat tgttgcttgt taaatgcaaa tattttgtat tatctggtaa
     4081 ggtgaagctt ttaggaatat aattatagtt ttgatctttt gaagaagcaa taacattaac
     4141 cttaccaaaa atttttgctt cacttattgc tttgcttccc caaatgccat tatcgcaata
     4201 tgcagcagtt tcattttcat ttaataaatt taatggaacc tgcataaact gtgtagtggc
     4261 accaccatgt aaaaaaagca cttcataatt gttgtttaac tgcatcaatt ctttaaccaa
     4321 caatttagat tcttctatta catctacaaa ccaatttgtt ctatgaccaa tttctaataa
     4381 agaaaggttt ataccattaa aatttattat tgcttctgag gcctgttcta acacttcttt
     4441 gggtaaaaca gatggaccag agttaaaatt atgtttcctc atttcggcac aaacataaaa
     4501 taaatcaata agtattttat gtttttaatt tattagactt ttattaataa aaataaagca
     4561 atctgaaatt tgtaaagtaa tctacaaatg ttaaaaaatg ttttttggta aactaatctt
     4621 ttctccgaaa tttattgcgt ggcaaaaaaa atattttact cttttttagc aataattgct
     4681 gttctacaat tgtttcaacc agatagaaat atatctactg aaaattttaa agtagaatta
     4741 gccaatcatt actctgtacc agatagtatt gaatctcttt taaatactgt ttgctacgac
     4801 tgtcatagta acaatacaga ttatccttgg tttgtaaata ttcaacctat tggttggtat
     4861 ttgcaaggaa agataaataa gggcaaaagc aaacttaatt tttctgaatt tggaagttta
     4921 tttaaagaaa aagcgataga aaagttagaa aatatagaag aggtaatgaa aaaaaatact
     4981 atgccattaa cctcttataa atggtacaat aaaaatgccg atttaactat tgaacaaaga
     5041 gaaatgatat ctaaatgggc tattaattta agtaagcaaa ttgcaagcga tagtactgca
     5101 gctttaaatg cagattcctt acctgttaaa ccactttaat taaaaatttt aaaattaaat
     5161 tcccaaaaaa ataatagaat tcactaaatt actaattttt ttagtatatt tgaactacaa
     5221 attttaggaa tttattttat ggtaaattat attaatgata gtacaaatca actagccaga
     5281 tataaaataa ctgcagaatg cacagagcca tttgaacacc ttattattgc aggtttttgc
     5341 tctaatgtgc aaacagaaaa tatattatac aggcacctac aaaatgtttg taaacaaagc
     5401 aaaggaaatt ttagtaatgc ccatacgcta attaatgata caataactca ctggcaaaat
     5461 acttttaacc tgcctgcggt acagtttctt aaaaatatca atacagaaaa aacagcttgc
     5521 agatataata aaatttgcct ttataaaatt aaccatcaag caaccgaaga actattgtat
     5581 aatatttatt tatagtaata agcaataact aaaaaattac aaacttgcct tttttaatct
     5641 tttaggtaat ttaatttcag tttgtatggt tttattgctt cttttatact ttagtttaaa
     5701 cgtatctcct tctttaaggt cttttaaatt tactttaaaa tcatccacac tcttcaattc
     5761 ttttccattt atttctgtaa taatatcctc ttcttttaac cctgcttttt ctgctggtga
     5821 ttcatcctct acatctaata cttttacacc attgccttct tccacatcct gaatttctaa
     5881 accaattttg gggcgacgag gcgagaaatt gccaccgtta tttttcatat caaacccttc
     5941 gtaaatatta tttaatgata ctacattagg gcctttgtat ttatctagta tggcagttgt
     6001 tgtttgtgtt ttaccatttc taatataagt tatagttact ttatcattag gtttatattt
     6061 tccaataatt ttatataaac tattagtatc actcacaatt tctttatcta tttgggttat
     6121 tatatcttct atttgtaaac ctgctttttc tgctgcactt tctttactta cattaacaac
     6181 aattgcaccc tccttttcga atttagttga aacgccaagg attgctttat taggtttcat
     6241 tttatcgata aatagttcat ttaattctct gaaattttca aaaaagtgct tgccattttc
     6301 ttgtccttta aatcttttac ctctaactat ttcaacatta tccaaagcat atttttcaac
     6361 aggaatgcca tttacagtaa tttggtcccc atctataact aaaacatatt tctcttcttt
     6421 ttctttttta gtaacagaag actctttatc ctttttctta tcattttgct cctcagtttt
     6481 ttgagcataa actaaaatag tagaagataa aaaaataact aatgtttgaa aaattctttt
     6541 tgtcataaca gaataattta atgatgtacg aatattttag tagaacaaat ataaaaaaga
     6601 atccgatata cgaaacattt cggaaatgtt aaatatttta aaatttgagg ttggggtaat
     6661 tatctattgt ttcaaataca aaatttgaat tttgttttaa tgttttaata aattcactca
     6721 accaattggc atattctgga gttctaaaca ttctatcatg cgttaaaata actatatgct
     6781 ttttagtaaa gctttgccca ctggctgcat ttttaacttg tgctgcaact tgttgagggc
     6841 tttgaacagg aaaagaatta cctttggtaa agttccattc tacatcccat ccaattacat
     6901 taaaaccagc atcatttaat ttttggcaaa ctggttttac taatttacta gtcctaattt
     6961 tatcttttgt aacccaagca ttgttacctg gtaatcttat tattttataa ggcacattta
     7021 aactttcttg tgctttataa aaatcttgct cggccattag tggatgagaa taaaaataaa
     7081 cgtatttctc gttagtatgc gtatagctat gattggataa tagaaattgt gggtatgcat
     7141 ttctaattgt ttttacagtt tgttgccccc aactatcatt tgcatgttta cccaccataa
     7201 aaaagcttgc ctttattcct tcttttttgc atacatccaa gcaattcatt gtaccatgcc
     7261 ctggcccatc atcaaatgtt aaataaatgt atgttttatt tgaatcgtaa ttgacaggtt
     7321 tcctttcaaa ctttattgca ttggagtcaa caataacctt aattgattct tttaattttg
     7381 cactatcatt tttacttgta agaacaggaa tattatttgc attattattt atgcaagaga
     7441 gaataaaaat ggagaaaaat aatagtaata atttggttgg gttgcctaac acattttttg
     7501 ggttcattga tactattttg taactaaatt atacaaaaaa tatataggag ctagctaaat
     7561 gattgttata atcttaattt gcttacttac gacaattgga agaatttatt gaactattaa
     7621 tatagtaagc ataattaatt aaatttgtgg tattcatttg taattattca ttattaaaat
     7681 taaatattat gtcttttact ttagcttctt taccttatgg ttacgatgcc ctagaaccac
     7741 acatagatac aacaactatg caaatacatc atggcaagca ccatcaggct tatgtagata
     7801 atttaaacaa agccattgct ggtacagatt atgaacaaaa aacattagat caaataaatg
     7861 ctattgctgg ttcaatatct gctgcagtta gaaataatgg tggaggtcat tggaaccatt
     7921 cttttttctg ggagagcctt gcaccaaaca gtggtggctt accaagtggc aatttagcag
     7981 atgctattaa tacagctttt ggtagttttg atgagtttaa agcaaagttt gccaatgctg
     8041 gtgccacacg ttttggaagt ggttgggctt ggttaattgt taaagaaggg aaattagaga
     8101 tttgctcaac acctaatcaa gataaccctt taatggatat tgctgaaaca aaaggaaccc
     8161 ctattttagg tgtagatgtt tgggagcatg catactattt aaaatatcaa aataaacgtg
     8221 ccgattattt aagtgccatt tggaatgtgg ttaactggaa taaagttgca gaaagatata
     8281 atgctgcaaa ataaattaat gcttgcaaaa aaaaacgagg cctactgtaa gcctcgtttt
     8341 ttttataagt aaaatctaat tgctatctta ttaaaaccac tgtacctttt ttgaaaatta
     8401 ccttacctgt ataatcaacc ccttgtgctg taaatacata agttccgcta gcttgaggtg
     8461 tgccatttac atttccatcc caacctttta agtattcttt tgtttcataa attaattgac
     8521 caaaacgatt gtagattcta aaaaaatcaa tttgagatat accaacagga ataggtttta
     8581 gaatatcatt ttttccatca ttatttgggg taaaagcagt tggtataaaa atatcgggtt
     8641 gcgttttaaa tattttaact actaattcat ccgaaccaat acagccttct ggccttttta
     8701 ctgttacttt atattttata gaatctgaat tgatatttag agtagcaatt gggttgaaaa
     8761 tagtagtgtt attgatacct gtgcttggag accaaacata agtactattg ttaggaattg
     8821 gacttgttac agcttgtaat tgcaagggtt gattttttac aactacagta tcattacctg
     8881 caaacactac cacttttggt attacattta cttgaataga atcgctaaat ggttttggac
     8941 aacctaatgt atcgttaaca gttataatat aaaaagtagt atcagtaggt tttactgttg
     9001 gcgaaagtga atttgcgtta gttataaaag ttgatggaga ccaataaaaa ctactgccaa
     9061 taaaattatt tgtacttaaa gtaatactat cgccataaca aatagaattg atggcattta
     9121 tagctacttt aggatatgga attggagtta ttactattgt atcataatct tgacattttc
     9181 ctaaattggc ggttacataa tatttggtaa tagccgtagg ttttactaaa ggaaatttaa
     9241 cttgaggcac aatttctcca gtagaagcag accaaacata actaagggca tggctaattg
     9301 tactcagtct aaaagtatcg gttaagcata ctgttgaatc tggccctgca gtgactgaaa
     9361 taaaatctaa cacgtttact ttaatagaat ctttactaat acaaccattg tcgtttacag
     9421 tgataacata ggttgtggta tcttttggat atacagaagg atttaaacta gtaggactaa
     9481 taatgttata gttaggtgcc caacttatgt tgccagttgt attagcaatt aagggcaatg
     9541 tatcaatact acatattaat gtatctttaa aaggcaggct aatggtaggt aaatctttaa
     9601 caataacatt tttatttacg gtatctaaac aaccttttgt attggttaca attaaagata
     9661 tgttgtaagt accaggggca ggatacttcc aagcagtatc ttttttatta gaagtatcgg
     9721 caacagtagt taaatctcca aaattccatc tccaactatt tacagtacca taaacagttg
     9781 ttgtggcatc taaaatattt attggcgaaa caacacatgt gccaactgta ttaatacctg
     9841 gcttaaaacc agggaaagca taaatatatt tataagaaga atcctggcat ccaccagtag
     9901 cggttacttt tagtttaatt aaatatttac ctgtatcgga ataagtatat gttggtgtag
     9961 gtactgttga tgtattacca tctccaaaag accatacata cgaaataatt gcagggttag
    10021 gagactgatt ttcgaagaag taagtaaatc catcgcaagt tctatcatct ggtcctgcat
    10081 taggttttac agaagagcag tttgcaactc ttaatataaa atcttttcta tgttcattaa
    10141 ttaaagtatc atttctccat tcttctacag ttacacatac aacatattta cctgcaatgg
    10201 gtggtgcttt tccacttatg attcctgttt ttgggttaat gcttgcacct gtgccaaaag
    10261 gattatttga actaaaaggt gcaggataat ttaatgatac aggatttaat acacttggtg
    10321 gtggtggatt tgggctagga tttggtccat cttggccatc atacgctgca cttaatttat
    10381 aggctaaaga atctccatct gtatctgatg cactaaagtc aagtaaaaaa tcggtagaga
    10441 tacaaactgt attcgtatct cttacagcaa aagttgggca gttattattg cctactggta
    10501 attgttttgt accaggaatt tttgttgtaa aagtagcccc catttggcta gaggagttgg
    10561 aaacattggt aatggatgtt ctggtatatc ttatccaaga aagtgtatag ccattttctg
    10621 ttttgggtaa atcaatattt gcctgaaata ttcctacttg ataacaaaca ctaacaaaag
    10681 gtaataaaca agggttggca ccagcagtat tatgcacttc gggaggtgta ccagaaaatt
    10741 gttgcgttaa cttttgtgtt gtaacaagtg ttaaaccagg attgttataa accccaatgg
    10801 ttacattttc tccatttaaa gcagcaaagt ttggaccagt tgggccacat tctctaaaca
    10861 atcgcatcgt aactaaatat ctatctgtat ttcctgcacc tggacctata taagtataaa
    10921 ataattcacc accagaaata tgctcggcat aagaaaaaaa ggtagtaaat aatgttatca
    10981 ataaaaataa aaacttcttc atcattttaa atactggggg ttgaatggtc aaaagtacat
    11041 acaactattt aatgtaaata ttttaaaaat agaggcaata ttagttagta tttataaata
    11101 ataaaagagt attattttaa tggctttgct gcattaaaaa tctaatttaa tgttaggttt
    11161 tggaaaatat ccttacaaaa tacttctata cttttattta acttatctgt tgcttctacc
    11221 atactcatgt gtcctacttc atcaaaaata tgcacataag atttttgggg taaatgtact
    11281 tgctgcaata catccttcat tggtgctggc acatcttctg taccaataat aaataaaacg
    11341 ggtactgatg catctttcaa aacctccgtt ctatctggtc tattcatcat tgcaaaataa
    11401 tattgttgca aagcctcatt tgtaaactta gcagatttat caattagctt attaatttga
    11461 ggtgcttgtt gcatactaaa ttttttacca aaataatttg gaataacatt ttttaaaaaa
    11521 gagaatgccc catattcttc tatcattgca atactctgca ttctattttc ttttttaatt
    11581 tcattatctt caaaagcagt ggagtgcaat agcccaaatg cttttaaata gtttggaaat
    11641 ttttcggcaa atgcaagtgt tatgtaacca cccatgctat gccccaataa aatacattca
    11701 ttaatggcta aaaaatttaa taatgctgct atacaatttg cataatctgt tattgcagct
    11761 tcatgaagca taattttatt aatctcaatt acacttctta atttaagtag gctactattg
    11821 ccagatccag gtaaatccgg tacaattact tgaaaattat ttttaagata attaacttga
    11881 ttattaaaaa ttgtggaatc ttcaccaaaa ccatgtaaaa gcaccaaggg ctttccagaa
    11941 ccataaattg tataagcaat attatgcccc ttataattaa aaatactctg ttgcattatt
    12001 gtgttttttt attttttctt aataaaataa acagaaaata accaattaat atgaatgcta
    12061 taattgaaaa aataaagtac aaataatctc catgttgtac ataaaaagat ttagcattat
    12121 taatttgtac agtttgtttt attgcacctt ccttattcca atctaaagtg tttaatacta
    12181 aaccatagtc attaattaca gcactaatgc cagtattggc acttcttaca atccaacatc
    12241 tagtttcaat agctcttaat ttggcataag ccaaatgctg cttatggcct gcagtattgc
    12301 cccaccaacc atcatttgta aggatggtaa ttaagtttgc accttttttt atataggtgc
    12361 ttaaatattc tccataaata ctttcataac aaataactgg tgctacatta agttgattat
    12421 ttatttgaaa aactttagag gtttcgtctt ttgcatagcc acctgttgtt ccaccaaatc
    12481 gttcaaaaac cggagaaaga atttttaaaa aacttggtaa agtttcaaca cctggtacta
    12541 atttactttt aatataaata ttcgaaactt tattgctatt aatggctatt gcagcattat
    12601 aactttcgta ataaacatct tgagtagttt tttgagaata ctttgtttcg ctactcatta
    12661 ttttataagt ttcaattcct gaaactaaag ttatttttgg atggttcttt aagaagtcaa
    12721 aaatgggttt atacacagtt gcatttgaaa ttgcatcttc tggaattgca gaagataaag
    12781 ccgtctctgg ccataatact aaaactgtgt tgctatcaat agaacttgca gttaaggata
    12841 ctaatttttc aatttggcta ttaatattac cactctcaaa cttcaggtaa gggtctatat
    12901 taggttgaat gattactaca ttttcattag cctttgcaat taatttttct ctgttattgg
    12961 ctatttgata agaaattatt attggaaaac taatggcaaa aattactgcc aataagtaac
    13021 caaattttac ttttgcagaa attgtactcc actgcttagt agtttcaaaa attaaaatat
    13081 tacaaaataa aatccataaa gttcctcctc caacacctgt atattcatac cattgaatcc
    13141 aataggggct attggcaaaa acatttccta aacttaacca aggccaactt aactcccaat
    13201 ttaaatggat atattcgaat aacatccaaa aacaaattaa tgccaggtaa ctaatttgtt
    13261 tgttgaattt atttttggta tgataataac cccaccaggg caagtacata acaaaactat
    13321 tagcaacaat agcaaaaatg ctacctacac ttgtactatt ccatatccac catgttgtaa
    13381 aaacattcca taatagaaga gcaataaata caaatgcaaa aaatagtttt ttattactaa
    13441 ccgtatctgc aataaaaagt aaagggataa aagcaaaaaa aacagcaatt gtaataggct
    13501 gtggtggcca agcaatatac atcattaagg ctgccacagt gcttaataat aaatatttaa
    13561 aagttttcaa taaagtattt ttaggctgcg aaagttcgga aaaaataaac aattctttca
    13621 attaaattaa tgcacaatgt gtaatgattg tattttagct tcaaaatatg tttttgaaaa
    13681 aaattttaat ttttgtaccc ggttttattt ggcttataat tagcttatgg ctttttacct
    13741 tgcctggtac tgctattcct aaatttgatt tatttacaaa gctgcaagga gacaaactaa
    13801 tacatgccat tatttttata gttttatgta tatcttttct acttccatta cataatattc
    13861 aaccttattg tgccaataat aaattttggt ttttattcat tatccttgtt tttggtatgt
    13921 atggtttggc aatagaattt gttcaagaaa aatttattaa atttcgttct tttgatgtag
    13981 gagatattgt ggcagattgg gttggttgct ttataggatt taaacttttt attagatatt
    14041 ggataaaaaa aataggtctc agtagaaacc gagaccgcaa ccaaaactaa ctgcttatga
    14101 gaaaaatttt aaatctttat tttattgttt gtaattagta acgcaattaa gatgccatta
    14161 ttataatctt tacatatttt aattgctaaa gaaatcttaa agcctacttt ttttatagtt
    14221 taatacttct aagaactaat tacattgcaa aaatgaatac ttaaccctaa aaattttgct
    14281 aacgttatat caacgaattg ttaattatta atattgtaat aaaaataaaa aagttaccta
    14341 ttaaagtgta acccattaag ccacaaataa ttaatttagc tattttttat tgattattct
    14401 gagtttgccc atattttctc tactaatgca ttaaatttag tacaaacctg acaaattgcc
    14461 ttttctgtca atatatgttt agcaagtgta taactgattt ttcaattaaa aaaagtagct
    14521 gaaaaaatca ttctataaaa aatttaaaaa atggtgtttc caaaatttca aatactcaaa
    14581 aagatacatt gtaatggaaa tatttattaa ggtttagatt tgaacaaact agttttacct
    14641 aattaatttt gtacaatggc tttatttaat ataagagttt atggcatttt gaaagatgat
    14701 aataataatg ttttattgag tgatgaatat atttatggaa attattttac aaaatttcct
    14761 ggtggtggtt tagagtttgg agaaggaaca agagaatgtt taataagaga atttaaagaa
    14821 gaaacagggc ttgatattat tgttggaaaa catatttaca ctacagattt ttttcagatt
    14881 tcagccttta acaataaaga tcaaataatt tctatttact attatgtgca tgcaagtaat
    14941 ttgagtgctt taaaaacatc ggctacgcca tttaatttta aagatgaaca attaatagat
    15001 aaaaaaggtg aagtggag
//
LOCUS       DAOQPK010000028        14917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_2037_length_14917_cov_5.008007, whole genome shotgun sequence.
ACCESSION   DAOQPK010000028 DAOQPK010000000
VERSION     DAOQPK010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 14917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14917
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2037_length_14917_cov_5.008007"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..1954
                     /gene="acs"
                     /locus_tag="PLU36_02480"
     CDS             20..1954
                     /gene="acs"
                     /locus_tag="PLU36_02480"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HMZ45648.1"
                     /translation="MSYPYQINSFEEYQQAYKKSIEQPEEFWASVAEHFTWRKKWDKV
                     LNWNFTEPTIEWFKGAKLNITENCIDRHLEKLGDKPALIWEPNNPEERVRIVTYNRLH
                     KRVCQVAQMLKNNGVKKGDRVCIYMGMVPELAYAVLGCARIGAIHSVVFGGFSAQSIS
                     DRLEDAKAEFIITCDGAYRGEKTIPLKSVIDDALIGNKTVKRIIVFTRTRTPVSMLKG
                     RDVWWEDEMDYAEEQVEKNGVVSFEPEIMDAEDPLFILYTSGSTGKPKGVVHTTAGYM
                     LFTNYSFVNIFNYKQGQIHFCTADIGWVTGHSYIVYGPLSAGATSVMFEGIPTYPDAG
                     RFWDIIDKHKINILYTAPTAIRSLMGFGTKPLENKNLSSLQVLGSVGEPINEEAWHWY
                     DEHVGKKRCPIVDTWWQTETAGILISNFAGITPSKPGWATLPMPGVQPILVDENGNEV
                     LPKENETIAGNLCIKAPWPGIIRTTYGDHERCRTNYFATYQNLYFSGDGALKDKDGNY
                     RITGRVDDVLNISGHRIGTAEVENAINMHVGVVESAVVGYPHAIKGQGIYAFVIVSSK
                     HSDDELTRKDILQTVTRVIGAIAKPDKIQFVAGLPKTRSGKIMRRILRKIAEGEVENL
                     GDTTTLLDPNIVDEIKNGRI"
     gene            complement(1977..2999)
                     /locus_tag="PLU36_02485"
     CDS             complement(1977..2999)
                     /locus_tag="PLU36_02485"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HMZ45649.1"
                     /translation="MKSLLLLTLICFIYNGKAQGLYPTYVNENVVYIDSTGSKKLETK
                     HKRCGLYQNGLAWVIINEAIGFMNTKGEVIIEPKYSETSGFYEGFASVVVDNAWGVIN
                     NKGETIVEPKYYEIGIYSDGMCKFKENKSLFSEWGFLDTKGKVAIRPTFENIDDFHEG
                     LAMFITDAKAGYIDTTGKVVIEPSFYYGAFFKINGKVVFEKGAFSNGLAAVEINNKFG
                     FIDKTGKVIIAAQYDDVKNFSEGLAAVKIKENWGFIDTKGSIIITPTYANVESFSEGL
                     AAVKIKENWGFINTKGKLIIPATLFGSIDIGIDLKFKDGVCQAMLTDKVWGYINKQGK
                     TLWKSK"
     gene            3160..4299
                     /locus_tag="PLU36_02490"
     CDS             3160..4299
                     /locus_tag="PLU36_02490"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HMZ45650.1"
                     /translation="MKVGIIGGGVAGLSTALALKKANISFHLFEQASAFKDIGAGVML
                     SSSTKYLLHQLGVGNDFEKASTLIKDFNITNHHLQTIKRIPSNEIGYGIHRARLIEVL
                     SKPLQQEDYTLNARIEKIEQNESGVTIDANNTTYFFDIVVAADGVHSVTRNKFSPHIQ
                     PSYTGQIMWRGIATLNLPDKFKNAAHEMWGKNKRFNIIHKGGDDYFWFCIRWIKDGIF
                     YEEENLKQFLHQEFNDFPEYAHAVIDADNNIIKTPLRHIKFNNLNNWYNNRIVFVGDC
                     IHACTPDIAQGACQAIESAYTLAACLKKYPNNFKDAFELYQFKRKPKTKFINNASFAF
                     SRFSHQRKKWQYNVLFSVFKLLPNSFLQKKFNKTNDIAYMNDLQL"
     gene            complement(4300..5115)
                     /locus_tag="PLU36_02495"
     CDS             complement(4300..5115)
                     /locus_tag="PLU36_02495"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMZ45651.1"
                     /translation="MQFNKKKAFRFIKVFIILYGGIGLIIYFFQDNFLFHPKKIAKDY
                     VYTFSTPFKEINIQYDSSTLFNTIQFLPKDTQAVKGVVLYFHGNRENINRYAPYVANF
                     TKNGYEVWITDYPTFGKSTGTLSEKVLETQALEIYKIAETKFKDSSIILYGKSLGTGL
                     AAYVASRKNCKQLILETPYSSITSLMKRYCFIYPIEKMIRYKLPVIEYIKKATAPIII
                     FQASEDWVIPNSNTEMLKVNLKATDKFVNIEGASHNTINNYPQYHKTLDSLLQ"
     gene            complement(5161..7785)
                     /gene="alaS"
                     /locus_tag="PLU36_02500"
     CDS             complement(5161..7785)
                     /gene="alaS"
                     /locus_tag="PLU36_02500"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HMZ45652.1"
                     /translation="MMTSAEIRQHFLNFFESKKHEIVASAPIVVKDDPTLLFTNAGMN
                     QFKDYFLGNKEPKNKRIADTQKCLRVSGKHNDLEEVGVDTYHHTMFEMLGNWSFGDYF
                     KTEAIAWSWELLTEIYKLDKDRLYVTIFEGDEKEGLPKDNEAYNEWKKVIAEDRILLG
                     NKKDNFWEMGDTGPCGPCTEIHVDCRMEEERKLVDGKLLVNNDHPQVIEIWNNVFIQF
                     NRKKDGSLEQLPAKHVDTGMGFERLVRVIQKKSSNYDTDVFTKTIAAVENITEKKYDF
                     GDNKQAIAFRVLADHIRAISFTIADGQLPSNTGAGYVIRRILRRAVRYYYSYLEYQQP
                     LLFQLVPVISTQFQNVFPELYKQKDFVSKVVKEEEEAFLRTLNKGLKLIDEIIVAANA
                     NQLKSINGNDAFELYDTFGFPIDLTRLIAAENNLSIDEEGFEIALQQQKKRSRAATII
                     DTEDWIIINEGNSVFVGYNQLSIETKILKYRKVKAKGKESYQIVLAETPFYAESGGQV
                     GDKGWIDFGNCTIEVFDIKKENNLSIHFVTTLPESINLTGNIIATVNKDLRKETANNH
                     TATHLLHAALRNVLGSHVAQKGSLVNHENLRFDFSHFAKVTDDEIAAIEKIVNTKIRE
                     NIPVIIKEMKKEEAVALGAMALFGEKYGDVVRVVIIDPNYSVELCGGTHVTNTGELGF
                     FKIKHETAVSAGVRRIEALSGAGVEEFINNQLLQLQAVKNLFNNPKELLKVIENNAIE
                     NKELKKKIESFEAKQIVALKAELNEKVEIINNTKFIGAIVDVNSADNLKKLVIELKQN
                     ADVAILATNIIGKSNVVVMINEAWENTKSLDATKIIKEHIAPVIKGGGGGSKNIATAG
                     GQEASNLPKVIDVVKALL"
     gene            complement(7869..8363)
                     /locus_tag="PLU36_02505"
     CDS             complement(7869..8363)
                     /locus_tag="PLU36_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HMZ45653.1"
                     /translation="MFYLLIPAIIITSTYNNYINKIKQPTTINCKVIGIKDGDTFEVL
                     FNNKKLVVRLAHIDCPERKQPFYQNAKQYASSLCFGKNVTLVHNNNFDRNKRLIAEVY
                     IGTLNINKNLVKSGLAWHFKKYSTNTEYAIIEMEAKRNKIGIWSENNPIAPWEWRKAK
                     KKNY"
     gene            8453..8851
                     /locus_tag="PLU36_02510"
     CDS             8453..8851
                     /locus_tag="PLU36_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HMZ45654.1"
                     /translation="MSSEKTRIDKYLWAIRLFKTRSLATEACDKGRVKLNGVTVKAAK
                     TVHLNEEYEIKTEARKWVIKVNGLLNQRVAYTEAIKFYTDITPPEEKATIEFKPVSFH
                     TGKILSKIGRPTKKQRRDIDNFINFDEDEN"
     gene            8841..9290
                     /locus_tag="PLU36_02515"
     CDS             8841..9290
                     /locus_tag="PLU36_02515"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HMZ45655.1"
                     /translation="MKIKFSIFIICMVAIFKVNAQEENPDKKNEKADSTYSIVQIPAS
                     FTGGAPAWRKYLQNNLDVSLGTKYIQIPKGQKSAKVTVIVSFIIDKEGKVSEVKLHDA
                     EPDHIPYIFIKEAIRIIQTGPKWVPAVQYGKLVKYRMLQPISWVVSE"
     gene            9309..9983
                     /gene="trmD"
                     /locus_tag="PLU36_02520"
     CDS             9309..9983
                     /gene="trmD"
                     /locus_tag="PLU36_02520"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HMZ45656.1"
                     /translation="MNIDIISVAPELLDSPFAHSIMKRAKEKKLLQIQVHNLRNWAIN
                     KHGQIDDYQYGGGAGMVMMCEPLVNAIEALQQQKKFDEIIFMTPDGEPFTQQKANQLS
                     LKENILIICGHYKGIDERIREHYVTLEISIGDYVLSGGELAAAVVVDAIGRIIPGVLN
                     DETSALTDSFQDNLLAPPVYTRPANFKGWQVPDVLLQGDTKKIEDWRLEQSIIRTEKK
                     RPNLLK"
     gene            complement(10287..10943)
                     /locus_tag="PLU36_02525"
     CDS             complement(10287..10943)
                     /locus_tag="PLU36_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45657.1"
                     /translation="MKFYNLIIRYRFWLSLVAIVGGVILNVTGTAGFWPTFPLYFIGA
                     IGLFSNFFIGPLRLIQEPMESGNVEEVKKILDTIWFPNLLFKPVRSTYYTIKGNLAMM
                     EQDFDTAEKHLKKSSDIGSPMPEAEGANKLQLGMMAMQRGDLKQGESYIRAAIRAGIP
                     DKESEAVAYLQMCQIFMNKREFRAAKDFFRKSKACKPKTQQVLDQIKEIEKYISRMPG
                     "
     gene            11147..11572
                     /locus_tag="PLU36_02530"
     CDS             11147..11572
                     /locus_tag="PLU36_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45658.1"
                     /translation="MPKLIILFFVSAVLFACNQNTSIEKKFDEDNYETTKETLAEKEK
                     KNPARFLQVENRERKNIIGQTVVIGHFTNTATVCTYKDVQIKLSFFSKTGTQVDEGME
                     TIYETIAPGKRVKFKTKYFAPKGTDSVSIKVIDAIGEIK"
     gene            complement(11575..12777)
                     /locus_tag="PLU36_02535"
     CDS             complement(11575..12777)
                     /locus_tag="PLU36_02535"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HMZ45659.1"
                     /translation="MPGYELWGEEEKKHVNDVLETGILMRYGFDTPRKGIWKSIELEK
                     AITETFGCKYAQLTSSGTAALTTALAALGIGAGDEVIMPSFTFVASFEAVLSVGAVPV
                     LVDVDKTLTLNPKAVKNAITANTKCIMPVHMCGSMADLDALQAICKEHNLFLLEDACQ
                     SIGATYKGKHLGTIGNAGTFSFDFVKTITCAEGGAVVTNNQTVYTNCDGFTDHGHDHK
                     GVDRGADLHPFIGYNFRISELHAAVGLAQIKRLSTFLHIQRNNHAALKNILQQVPEVS
                     FREIPDPNGDSCTFLSWFLPTEEITKAFVAEMKAQGILAGNFYWYANNWHYIKQWQHL
                     QEAKSLYNYNPQQKDALLKLPSQNFSDSDAILSCCISTAISLVWINEQIIEKGTKMVA
                     AINKALGK"
     gene            12918..13844
                     /gene="rsgA"
                     /locus_tag="PLU36_02540"
     CDS             12918..13844
                     /gene="rsgA"
                     /locus_tag="PLU36_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HMZ45660.1"
                     /translation="MKALVYKSTGSWYIVKNSFGKQYNARIKGVLKIDGITSTNPIAV
                     GDEVIIEIENEIENTATIIEVCTRKNYVARQSPHNKRQHHIIASNLAQSLLFATITNP
                     VTSTGFIDRFLCSCEMFHIPAVIVFNKSDLYKEKDVEKFEQLKNIYTTVGYKVILTSL
                     INGNGIEEIKNILSNKTTLLSGHSGVGKSSFLNIVFPELNLRTQEVSSWSGKGLHTTT
                     FAEMFDIKDEGRIIDTPGIRELGLTGITKQELSGYFPEIKAEISHCKFNNCMHINEPA
                     CAVKNALQQGKIFEERYINYLKILDTIKDNNY"
     gene            complement(13841..14131)
                     /locus_tag="PLU36_02545"
     CDS             complement(13841..14131)
                     /locus_tag="PLU36_02545"
                     /inference="COORDINATES: protein motif:HMM:NF023724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3630 family protein"
                     /protein_id="HMZ45661.1"
                     /translation="MSYQLTNFSGAVEAVIDDNCGISKFHNIVHIMLQELKVKFLAKE
                     DDSDTVDWHFKYKGRPLTLHYNIYNGVSVFTQHSKNNSAVNEVAKYLESKTF"
     gene            complement(14209..14451)
                     /locus_tag="PLU36_02550"
     CDS             complement(14209..14451)
                     /locus_tag="PLU36_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45662.1"
                     /translation="MKQFKAVIIILILAVVLSSCNFFRNIFGGGDKYGCPTSVNNIGA
                     EKLSGDYKPKNREEAKAIKRAAKANKKAAKRNPYMY"
     gene            complement(14586..>14917)
                     /locus_tag="PLU36_02555"
     CDS             complement(14586..>14917)
                     /locus_tag="PLU36_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HMZ45663.1"
                     /translation="YQKNATNIGNNVSIGHNAIVHGCTIHDNVLVGMGAIVMDRCIVH
                     SNSIIAAGAVVLEGTVIEEGSIYAGVPAKKVKEISQDLIKGEIDRIANNYIQYASWFE
                     KYNDAAK"
BASE COUNT         5086 a         2327 c         2280 g         5224 t
ORIGIN      
        1 ttattaatta aagaagaata tgtcatatcc ttatcaaata aattcttttg aagaatacca
       61 acaagcatat aaaaaaagta ttgaacagcc agaagagttt tgggcatcag ttgcagagca
      121 ttttacctgg agaaagaaat gggataaagt gttaaactgg aattttacag aacctactat
      181 agaatggttt aagggagcaa agctgaacat aacagagaat tgtattgata gacatttaga
      241 aaaattaggc gataagcctg ccttaatttg ggagcccaat aacccagagg aaagagtaag
      301 aattgttact tacaatagat tacataaaag agtttgccag gttgctcaaa tgcttaaaaa
      361 taatggtgta aaaaaaggcg atagagtttg tatttacatg ggtatggtac cagaattagc
      421 ttatgctgtg ttaggttgtg ccagaattgg agcaatacat agtgttgttt ttggtggctt
      481 tagtgcacaa agcatttctg ataggttgga agatgctaaa gcagaattta ttattacttg
      541 tgatggagcc tataggggag aaaaaacaat accattaaaa tctgtaatag atgatgcttt
      601 aataggaaat aaaacggtaa aaagaataat cgtttttacc cgaacaagga cacctgtatc
      661 tatgttaaaa ggtagagatg tttggtggga agacgaaatg gattatgctg aagagcaagt
      721 ggaaaaaaac ggagttgtaa gctttgagcc tgaaataatg gatgcagaag accctttatt
      781 tatcttatat accagtggca gtactggcaa accaaaagga gttgtacata ccactgctgg
      841 ttacatgctg tttaccaatt attcttttgt aaatattttt aattacaaac aaggtcaaat
      901 tcatttttgt actgcagata ttggttgggt tacagggcat agttacattg tgtatgggcc
      961 attgagtgct ggtgcaacaa gtgtaatgtt tgaaggtatt ccaacctacc cagatgcagg
     1021 aagattttgg gatattatag ataaacataa aattaatatt ttatatactg caccaactgc
     1081 tataagaagt ttaatgggtt ttggaacaaa gccattagaa aataaaaatt taagtagttt
     1141 acaagtatta ggttcggttg gtgaacctat taatgaagaa gcatggcatt ggtacgatga
     1201 gcatgttgga aaaaaacgtt gcccaattgt agatacttgg tggcaaacag aaactgcagg
     1261 tattttaatt tcaaactttg ctggcattac accatctaaa ccaggttggg ctacattgcc
     1321 tatgcctggt gtacaaccta ttttagttga cgaaaacggc aatgaagttt taccgaaaga
     1381 aaatgaaacc atagcaggaa acctttgtat aaaagcacct tggccaggta ttattagaac
     1441 aacttatgga gatcatgaac gatgcagaac gaattatttt gcaacctatc aaaatctgta
     1501 tttttctggc gatggtgcat tgaaagataa agatggtaac tacagaatta ctggcagggt
     1561 agatgatgtt ttaaatatta gtggacatag aattggtact gcagaagttg aaaatgccat
     1621 taatatgcat gtaggtgttg ttgaaagtgc agtagtgggt taccctcatg caattaaagg
     1681 acaaggaatt tatgcttttg ttatagtaag ctcaaaacat agtgatgatg aacttaccag
     1741 aaaagatatt ttacaaactg taacaagagt aattggtgct attgcaaaac cagataaaat
     1801 tcagtttgta gccggtttgc ctaaaacaag aagtggtaaa ataatgcgta gaatattaag
     1861 aaaaattgct gaaggagaag tagaaaattt aggcgatact actactttgt tagaccccaa
     1921 tattgtagat gaaattaaaa acggaagaat ataaattctt ccgtttttaa tactctttac
     1981 ttactcttcc aaagtgtttt tccttgttta tttatataac cccatacttt atcagttaac
     2041 atagcttggc aaactccatc tttaaacttt aaatctattc caatatcaat agagccaaat
     2101 aaagttgcag gtataattaa tttgcctttg gtattaataa agccccagtt ttcttttatt
     2161 ttaactgcag ccaaaccttc gctaaagctt tcaacgtttg cataagtagg tgtaataatt
     2221 atacttcctt tagtatctat aaagccccag ttttctttta ttttaactgc agccaaacct
     2281 tcactgaaat tttttacatc atcgtattgt gctgcaataa ttacttttcc tgttttatct
     2341 ataaagccaa atttattatt tatttctacc gcagctaagc cattactaaa tgcacctttt
     2401 tcaaaaacta ctttaccatt tattttaaaa aatgcaccat aataaaaaga aggttcaatt
     2461 actactttac cagttgtatc tatatagcct gcttttgcat ctgtaataaa catggctagc
     2521 ccttcatgaa aatcatcaat attttcaaaa gttggcctaa tagccacttt gccttttgta
     2581 tctaaaaagc cccattcaga aaataagctt ttattctctt taaacttaca cattccatcg
     2641 ctataaattc caatttcata atatttaggc tctactattg tttctccttt gttattaatt
     2701 actccccagg cattatcaac tacaacgctt gcaaatcctt cataaaaacc agatgtttca
     2761 gaatattttg gctctattat cacttctcct tttgtattca taaagccaat agcttcgtta
     2821 ataattaccc atgctaaacc attttggtat aaaccacaac gtttgtgttt tgtttctaac
     2881 tttttacttc ccgtagaatc aatatataca acattttcat taacataagt agggtacaag
     2941 ccttgtgcct tgccattata aataaagcaa attagtgtta gcagaagtaa acttttcatt
     3001 gtaagtttta taatatttta tttacattat aaggtataaa gtatccacat taatgtaatc
     3061 aaatttcatt caactcttct tatattcata tacccaataa atggtatttt atggctaaat
     3121 ttattttgta cttataaaaa tagaaaccaa atttactgca tgaaagttgg cataataggt
     3181 ggaggagttg caggattaag tactgcattg gctcttaaaa aagcaaatat ttcatttcat
     3241 ttatttgaac aggcttctgc ttttaaagat attggtgcag gtgttatgct aagtagtagt
     3301 actaaatatt tattacatca attaggtgta ggaaatgatt ttgaaaaagc atctacttta
     3361 attaaagatt ttaatattac taaccatcac cttcaaacaa ttaaaagaat accttctaat
     3421 gaaattggtt atggaataca tagagcaagg ttgatagaag tattaagcaa acctttacag
     3481 caagaggatt atacattaaa tgcaagaatt gaaaaaatag aacaaaacga gagtggcgtt
     3541 acaattgatg caaataatac aacctatttt tttgacattg tagtagctgc cgatggtgtg
     3601 cactctgtta caagaaataa attttcgcct cacattcaac caagttatac agggcaaata
     3661 atgtggcgtg gtattgcaac attaaaccta cccgataagt ttaaaaatgc agctcatgaa
     3721 atgtggggta aaaacaagcg atttaatatt attcataaag ggggggatga ttatttttgg
     3781 ttttgcatac gttggataaa agatggaatt ttttacgaag aagaaaatct aaaacaattt
     3841 ttacaccagg agtttaatga ctttcctgaa tatgcccatg cagtaattga tgcagacaat
     3901 aatattatta aaacaccatt aaggcatatt aaattcaata atttaaataa ttggtataat
     3961 aatagaatag tttttgttgg agattgtatt catgcttgta caccagatat tgcacaaggg
     4021 gcatgccagg caatagaaag tgcatacacg ttggctgctt gtttaaaaaa atatcccaat
     4081 aattttaaag atgcttttga gctttatcag tttaaaagaa agcctaaaac taaatttatt
     4141 aataacgctt catttgcatt tagcaggttt agtcatcaac gaaaaaaatg gcagtataat
     4201 gttttatttt ctgtgtttaa acttttgcca aattcttttc tgcaaaagaa atttaataaa
     4261 accaatgata ttgcttatat gaatgatttg caattgtagt tattgtaata aactatctaa
     4321 ggttttatga tattgaggat aattattaat ggtattgtga gaagcacctt caatatttac
     4381 aaacttatcg gttgccttta agtttacttt tagcatttca gtattgctat tgggtatcac
     4441 ccaatcttcg cttgcttgaa aaataattat gggtgctgtt gcttttttta tatattcaat
     4501 tactggcaac ttataacgaa tcatcttttc tataggataa ataaaacaat atctcttcat
     4561 taaagaagtt atactgctat aaggagtttc taaaattagt tgtttacaat tttttcttga
     4621 tgcaacataa gcagcaaggc ctgtaccaag gcttttgcca tataaaataa tagaagaatc
     4681 tttaaacttt gtttctgcta ttttgtatat ttctaatgct tgagtttcta aaactttttc
     4741 agaaagggta ccagtgcttt tgccaaatgt agggtaatca gttatccaaa cttcgtagcc
     4801 attttttgta aaattagcaa cataaggggc atatctattt atattttctc tattgccatg
     4861 aaagtataaa acaactcctt ttacagcttg ggtatcttta ggtaaaaatt gaattgtatt
     4921 aaataaagtg ctactatcgt attgaatatt tatttcttta aaaggagtgg aaaaagtata
     4981 tacataatct ttagctattt tttttggatg aaacaaaaaa ttatcttgaa agaaataaat
     5041 aattaaacct ataccaccat ataaaataat aaaaactttt ataaaacgaa aagctttctt
     5101 tttgttgaat tgcatggtat tttaattaag aaatagtgta tggtatttta aaattttaat
     5161 ttatagcaaa gccttcacaa catctattac tttaggtaag ttacttgctt cttgcccacc
     5221 tgctgttgca atatttttag agccaccacc accaccttta ataacaggtg ctatatgttc
     5281 tttaataatt tttgtagcat ctaatgattt agtattttcc caagcttcgt ttatcattac
     5341 taccacattt gattttccta taatattggt agcaagtatt gctacatctg cattttgctt
     5401 taactcaata actaattttt ttaaattatc agcactgttt acatccacaa ttgcaccaat
     5461 aaatttagta ttgtttataa tttctacttt ctcattaagc tctgctttta atgctacaat
     5521 ttgttttgct tcaaagcttt ctattttctt ttttagctct ttattttcta tagcattgtt
     5581 ttcaataact tttaataatt ctttagggtt attaaataaa ttttttacag cttgtaattg
     5641 taaaagttgg ttattaataa actcctccac acctgcacca cttaaggctt ctattctgcg
     5701 tacgcctgca gaaacagcag tttcatgttt aattttaaaa aagcctagtt ctcctgtatt
     5761 ggttacatgg gttcctccac atagttctac agaataattg ggatctataa ttactactct
     5821 tactacatcg ccatattttt ctccaaataa agccatagca ccaagtgcaa cagcctcttc
     5881 ttttttcatt tcttttataa taacaggaat attttctcta atttttgtgt taacaatttt
     5941 ctcaatggct gcaatttcat catcagtaac ttttgcaaag tgagaaaaat cgaaacgtaa
     6001 gttttcatga tttactaagc tacctttttg agccacatgg ctacctaata catttcttaa
     6061 agctgcatgt aataagtgtg tggcagtatg gttattggct gtttcttttc ttaaatcttt
     6121 atttaccgta gcaataatat tacctgttaa atttatgctt tctggtaggg ttgttacaaa
     6181 gtgaatggat aagttgtttt ctttttttat atcaaaaact tctattgtgc aatttccaaa
     6241 atctatccaa cctttatcgc ctacttggcc tccactctct gcataaaaag gtgtttctgc
     6301 taaaactatt tgataacttt cttttccttt tgcttttact tttctgtact ttaatatttt
     6361 agtttctatt gataactgat tgtagccaac aaaaacagag tttccttcat taattattat
     6421 ccaatcttct gtatctataa tagtggctgc tctagatctt tttttctgtt gttgtaatgc
     6481 aatttcaaaa ccttcttcat caatagataa attattttct gcagcaatta atcttgttag
     6541 gtctatagga aaaccaaagg tatcgtacaa ctcaaaagca tcgttgccat taattgattt
     6601 tagttggttt gcattagctg ctacaataat ttcatcaata agttttaagc ctttatttaa
     6661 ggttcttaaa aaagcttctt cttcttcttt tacaacctta cttacaaagt ctttttgttt
     6721 gtataattca ggaaaaacat tttgaaattg tgtagatata actggaacaa gttgaaaaag
     6781 taaaggctgt tgatactcta aataagaata ataataacga acggcacgtc ttaatattct
     6841 tctaattaca tatcctgctc cagtattgct tggtaactgt ccatcggcaa tggtaaaact
     6901 aattgcccta atatgatctg ctaatacacg aaaagcaata gcttgtttat tatctccaaa
     6961 atcgtatttt ttttctgtta tattttctac agcagcaatg gttttggtaa aaacatcggt
     7021 atcataatta gatgattttt tttgaataac ccttactaat ctttcaaagc ccattcctgt
     7081 atctacatgc tttgcaggta actgttctaa actgccatct ttttttctgt taaattgtat
     7141 aaatacgttg ttccatattt ctattacttg tgggtggtcg ttgtttacta ataatttccc
     7201 atccactaat tttctttctt cctccattct acaatcaaca tgtatttcag tacaagggcc
     7261 acatggtcct gtatctccca tttcccaaaa attatctttt ttgtttccta acaaaattct
     7321 gtcttctgca attacttttt tccattcatt gtaagcttcg ttatcttttg gcagtccttc
     7381 tttttcatct ccttcaaaaa tggtaacata caaacggtct ttatctaatt tatatatttc
     7441 tgtaagtagc tcccaactcc aggcaatcgc ttctgtttta aaataatctc caaaactcca
     7501 gttgcctagc atttcaaaca tagtatgatg ataggtgtca acacctactt cttccaaatc
     7561 attatgttta ccgcttactc gtaagcattt ttgagtatct gcaatgcgtt tgtttttagg
     7621 ttctttatta cctaaaaaat aatctttaaa ctggttcata ccagcattgg taaataataa
     7681 agttgggtca tctttaacaa caattggtgc agaagcaact atttcatgct ttttactttc
     7741 aaaaaaattt aaaaaatgtt gtctaatttc agccgaagtc atcatataaa agtaggtatt
     7801 aaaaagtgag caaagataag gccgaggctt ttatatgttt agtttattca ataatttaaa
     7861 aggcttattt agtaattttt ctttttagcc tttctccatt cccaaggtgc aattgggttg
     7921 ttttcactcc aaataccaat tttgtttctt ttagcctcca tttcaataat ggcatattct
     7981 gtattggtag agtatttttt aaaatgccat gcaagtccac tcttcactaa atttttattt
     8041 atatttaaag taccaatata cacttctgca ataagtcttt tatttctatc aaaattatta
     8101 ttatgaacca gagttacatt ttttccaaag cataaacttg aggcatattg tttggcattt
     8161 tgatagaatg gctgtttcct ttcagggcaa tcaatatgtg caagtcttac tactaatttt
     8221 ttattattaa acaatacttc aaaagtgtct ccgtctttaa ttcctattac tttacaatta
     8281 atagttgttg gctgttttat tttgttgatg taattattat acgtactagt aataatgatt
     8341 gctggtatta ataaataaaa catgcttatt tttagaaaag aaatcatttg tatttttcca
     8401 ctacaatttt taaaaaagaa gttaaatata aagtattatt ttttgagaaa ttatgagttc
     8461 agaaaagaca agaatagata aatacctttg ggcaataagg ctttttaaaa caagaagttt
     8521 agctaccgaa gcttgtgata aaggcagagt aaaattgaat ggagttactg taaaagctgc
     8581 taaaacagta cacttgaatg aagagtatga aattaaaaca gaagcaagga aatgggtaat
     8641 aaaagtaaac ggcttattaa accagcgtgt tgcttatacc gaagcaatta aattttatac
     8701 tgatattaca ccaccagaag agaaggcaac catagagttt aaacctgttt cttttcatac
     8761 aggtaaaatt ttaagcaaaa taggaaggcc aactaaaaag caaagaagag atattgataa
     8821 ctttataaat tttgatgaag atgaaaatta aattttcaat atttataata tgcatggttg
     8881 ctatttttaa agtaaatgct caagaagaaa accccgataa gaaaaatgaa aaagcagact
     8941 ctacctacag tatagtgcaa atacctgcta gttttacagg aggagcacct gcatggcgta
     9001 aatatttgca aaataattta gatgttagct tgggtacaaa gtatatccaa atacctaagg
     9061 ggcaaaaatc agcaaaggta actgttatag taagttttat tattgataaa gagggtaagg
     9121 tatctgaagt aaaattgcat gatgcagagc cggaccatat accttatatt tttattaaag
     9181 aagccataag aataattcaa actgggccaa aatgggtacc tgcagttcaa tatggcaaac
     9241 ttgtaaaata tagaatgtta caaccaatta gctgggtagt aagtgaatag taggtttata
     9301 tttgcaatat gaatattgat ataatttctg tagctccaga attattagac agcccttttg
     9361 ctcatagcat aatgaaaaga gcaaaagaaa aaaaattact acaaattcaa gtacataatt
     9421 taagaaactg ggctatcaac aaacatggcc agattgatga ttaccaatat ggtggtggtg
     9481 caggtatggt aatgatgtgt gagcctttag taaatgcaat agaggcttta caacaacaaa
     9541 aaaaatttga tgaaataatt tttatgacac cagatgggga accttttact caacaaaaag
     9601 ccaatcaatt atcgttaaaa gaaaatatat taattatttg tggccactat aaaggaattg
     9661 atgaaagaat aagggaacat tatgtaacac tagaaatttc tattggtgat tatgtgttaa
     9721 gtggtggaga gttggctgct gcagtagttg tagatgctat tggaagaata atacctggtg
     9781 ttttaaatga tgaaacttct gcattaactg attcttttca agataattta cttgcaccac
     9841 ctgtttatac caggccagct aattttaaag gttggcaggt accagatgtt ttattacaag
     9901 gcgataccaa aaaaattgag gattggcgtt tagagcaatc cattattaga acagaaaaaa
     9961 aacgcccaaa ccttttaaaa tgattaatta atcctttttg gttaatgcat aaacttttag
    10021 ggatattaaa ttgatactga atgaaaaatt tgtctttttg gggtattaca aaatctaata
    10081 cattaacatt tatttaaaag ttggtactac ttttaatcaa caaaagaaaa tccggtgagc
    10141 tttatgaaaa caaaagttcc tgtttaaaat ccaagatttc tctataactt aaaaatccta
    10201 tgtagaacca acaccggtct aaaagcatta aaatgcaaaa caaaaaatcc ccaaaatttt
    10261 ggggattttt tgttttaatt agttaattaa ccaggcattc ttgaaatata tttttcaatt
    10321 tcttttattt ggtctaaaac ctgctgggtt tttggtttac aagctttaga ttttctaaaa
    10381 aaatctttag cagctctaaa ctctctcttg ttcataaata tttggcacat ttgtaaataa
    10441 gcaacagctt cactttcttt atctggtatg ccagctctaa ttgctgctct tatataactt
    10501 tcgccttgtt ttaaatcgcc acgttgcata gccatcatgc ctaattgtaa tttattagct
    10561 ccttctgctt ctggcattgg gctaccaata tcactacttt ttttcaaatg tttttcggca
    10621 gtatcaaagt cttgctccat cattgccaaa ttacctttaa tagtgtaata ggtagaacgt
    10681 acaggtttaa ataataaatt aggaaaccaa atggtatcta aaattttctt aacctcctca
    10741 acatttcctg attccattgg ctcttgaatt aatcttaatg ggccaataaa aaagttacta
    10801 aataaaccaa tagcaccaat aaaataaagt ggaaatgtag gccaaaagcc tgctgtgcca
    10861 gttacgttta aaataactcc tcctacaatg gctactaaac ttaaccaaaa acgataccta
    10921 ataattaaat tataaaactt catattttta agaaaaattt attaaagggg gcaaagttaa
    10981 cttttaaaga ttcataacca ttattttata ccattgcttt ttttaataaa gtttatttaa
    11041 cttattattg aataaattta tgcttagaaa actaggaagc ataaaacttt gcaaaaaata
    11101 aaaaactaat ttattgcagc tttatataaa aattaaaaat aaactcatgc caaaactaat
    11161 aattcttttt tttgtaagtg ccgtgttgtt tgcttgtaat cagaatacat ctatagaaaa
    11221 aaaatttgat gaagataatt atgaaacaac caaagaaact ttagcagaaa aagagaaaaa
    11281 gaaccctgcc agatttttac aagtggaaaa ccgagaaaga aaaaatatta ttgggcaaac
    11341 agttgttatt ggccatttta caaatacagc aacagtttgc acatataaag atgtacaaat
    11401 aaaacttagt ttttttagta aaactggtac acaggtagat gaaggaatgg aaactattta
    11461 tgaaaccatt gccccaggta aaagagtaaa atttaaaaca aaatattttg caccaaaagg
    11521 caccgatagt gtaagcatta aagtaataga tgcaatagga gaaataaaat aaaattattt
    11581 acctaaagct ttatttattg ctgctaccat tttagtacct ttttcaatta tttgctcatt
    11641 gatccatact aaacttatag ctgtagatat acaacaactt aaaattgcat cactatccga
    11701 aaaattttgt gatggtaatt taagtaaagc gtctttttgt tgtggattat agttgtataa
    11761 actttttgct tcttgtaaat gctgccattg tttaatatag tgccagttgt tggcatacca
    11821 gtaaaaatta cctgctaaaa ttccttgtgc tttcatttct gctacaaagg cttttgtaat
    11881 ttcttcggtt ggtaaaaacc aacttaaaaa ggtgcaacta tcgccattag ggtctggtat
    11941 ttctctaaaa ctaacttcgg gtacttgttg taaaatattt tttaatgctg catggttatt
    12001 acgctgaata tgtaaaaaag tagaaagcct ttttatttgt gccaatccaa cagcagcatg
    12061 taactcacta attctaaaat tatatccaat aaatggatgt aggtctgcac ctctgtcaac
    12121 tcctttatgg tcatgtccat gatcagtaaa accatcacaa ttagtataaa ctgtttggtt
    12181 gtttgttact acagcacctc cttcggcaca agtaattgtt tttacaaaat caaaactaaa
    12241 agtaccagca ttgccaattg tgcctaagtg tttaccttta tatgttgcac caatactttg
    12301 gcaggcatct tctaataaaa ataaattatg ttccttacaa attgcttgta gtgcatctaa
    12361 atctgccatg ctgccacaca tgtgtacagg cattatacat tttgtatttg cagttatggc
    12421 attttttaca gcctttggat ttaaggttaa agttttatct acatctacta aaactggaac
    12481 agcaccaaca cttaatactg cttcaaaact tgccacaaaa gtaaagcttg gcattataac
    12541 ttcatctcct gcaccaatac ctaatgcagc caaagcagta gttaatgctg ctgtgccact
    12601 acttgttagt tgtgcatatt tacaaccaaa ggtttcagta atggcctttt ctaattctat
    12661 acttttccat attccttttc ttggtgtatc aaaaccataa cgcattaata ttcctgtttc
    12721 taatacatca tttacatgtt ttttctcttc ttctccccaa agttcataac ctggcataat
    12781 gttgtaattt atttttttat aaagagcaac aaaattagag aaacaaagca actaaaagca
    12841 atagtttttt tttagcaatt cattttactt ttaaacaaat aaaaaattaa ttactgtact
    12901 tattaacttc gtacacaatg aaagcccttg tttataaatc tacaggaagt tggtatattg
    12961 taaaaaatag ttttggcaag caatacaacg cacggataaa aggggtatta aaaattgatg
    13021 gcattacatc taccaatcct attgcagttg gagatgaagt gataattgaa attgaaaatg
    13081 aaatagaaaa tactgcaaca ataattgaag tgtgcactag aaaaaattat gtggctcgcc
    13141 aatctcctca taataaaagg caacatcata ttattgcatc taacttagct caaagcttac
    13201 tttttgctac tataactaat cctgtaactt caactgggtt tatagatagg tttttatgct
    13261 cctgcgaaat gtttcacata cctgctgtaa tagtttttaa taaatcagac ttatacaagg
    13321 aaaaagatgt tgaaaaattt gagcaactaa aaaatattta tacaacagta gggtataagg
    13381 taatacttac aagtttaatt aatggcaatg gtattgaaga aataaaaaat attttaagta
    13441 ataaaacaac attgttaagt gggcatagcg gcgttggcaa atcttctttt ttaaatattg
    13501 tttttcctga attaaattta aggacacaag aggttagcag ttggagtggt aaaggcttac
    13561 acacaactac atttgccgaa atgtttgaca ttaaagacga aggtagaatt attgatacgc
    13621 caggtattag agagcttggc ttaactggta ttaccaaaca ggaattgagt ggctattttc
    13681 cagaaattaa agcagaaatt agccactgta agtttaataa ttgcatgcat ataaacgagc
    13741 cagcttgtgc tgtaaaaaat gcattacaac agggcaaaat ttttgaggaa aggtatatta
    13801 actacctaaa aattttagat acaataaagg ataataatta ttagaaggtt ttactttcta
    13861 aatatttagc aacttcgtta acagcagaat tatttttact gtgttgcgta aatacagaaa
    13921 cgccattgta aatattataa tgtaaagtaa gcggacgacc tttgtattta aaatgccaat
    13981 ctactgtatc gctatcatct tcttttgcta agaactttac ttttagctct tgtagcataa
    14041 tgtgtacaat gttatgaaat ttactaattc cacaattgtc gtcaataaca gcctccacgg
    14101 ctcctgaaaa gttggttaat tggtagctca tagaaataaa gttttatgga tttaaaaatt
    14161 ggatataaag attttttaac tattttctat taaccaagaa tacccttttt aatacatgta
    14221 tgggtttctt tttgctgcct ttttattggc tttagctgct ctttttattg cttttgcttc
    14281 ctctctattc tttggtttat aatcgcctga taacttttct gctcctatat tatttacact
    14341 tgttgggcag ccatatttat ctcctcctcc aaaaatattt ctaaaaaaat tacaagaact
    14401 tagaacaaca gccaaaataa gtataattat aactgcttta aattgtttca tgaatttatt
    14461 ttttgaactg caaagttatc atataccaat aagttcagta gtaattttta tcaaaaatct
    14521 ataaaaagct ttaaaactgg gttaaaactt ttgtttgtac attatataac ggcataatat
    14581 aaaatttatt ttgctgcatc attatatttt tcaaaccaag aggcgtattg aatataattg
    14641 ttggcaatac ggtctatttc gcctttaatt aaatcttgag aaatttcttt aacttttttt
    14701 gctggcacac ctgcataaat acttccttct tcaataacag tgccttctaa aactactgca
    14761 cctgcagcaa taatactgtt actatgtaca atgcatctat ccattactat ggctcccatt
    14821 cctactaaaa cattatcgtg tattgtacag ccatgcacaa ttgcattatg gccaatggaa
    14881 acattgtttc caatattggt agcatttttt tgataag
//
LOCUS       DAOQPK010000029        14849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_2051_length_14849_cov_4.868460, whole genome shotgun sequence.
ACCESSION   DAOQPK010000029 DAOQPK010000000
VERSION     DAOQPK010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 14849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14849
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2051_length_14849_cov_4.868460"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..1478)
                     /locus_tag="PLU36_02560"
     CDS             complement(210..1478)
                     /locus_tag="PLU36_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45664.1"
                     /translation="MSDFTHHINYTAADINNYLQGKMSNAERNAFEKAALADPFLADA
                     LEGFEIAETTKALQMLKETDNLISASALKQNKNYNLADIENYLQNNMGNAERNAFEKA
                     TLADPFLADALEGFENANLTEAKKYIGETATTILSKEVGKAKVVTMPIYKKQWLRIAA
                     GFIIMIGAGATIWLLNNNTSTKKENTLAAITKEEIKQTPNTISALPTEKENTSEKIVN
                     NHQQNASNKKEKVKIEDFNIINDNALISSPQAAIKTTESSAGIFSTEDALNKNKPKDI
                     SVKSMVGEPPAFPNNGAVNAQNSRNIEERNAIASNLAEASKSKKATAAVLKISDNEQN
                     NKGITPIGGWKKFDDYINTKRAALHFTNELVESVIVTFLIDKEGKPYQIQAQSSIPEK
                     SVRAIELIENGVNWQVKNTTNNKAVVIIKL"
     gene            complement(1471..2040)
                     /locus_tag="PLU36_02565"
     CDS             complement(1471..2040)
                     /locus_tag="PLU36_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ45665.1"
                     /translation="MALTVKHIDNTADDNSLMLLYQQSLDQKSLATLFLRYNDLVFGT
                     CMKYFKDEETSRDAVMDIYQELVQKLPNHKVDNFKSWLYVVTKNHCLMQLRKNKKTTT
                     VEFDGQFMQSEDFSHLDNVLEKEQKLNSLEGCIETLNEEQKKSIQLFYLENKCYNEIA
                     DITGIEWNKVRSLIQNGRRNLKICMDKNV"
     gene            2196..3113
                     /locus_tag="PLU36_02570"
     CDS             2196..3113
                     /locus_tag="PLU36_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ45666.1"
                     /translation="MKSAIFLQIATPCNENWEKMTPMEKGKFCASCAMQVTDFTLMTD
                     KEILQYLSTTRGKVCGRIHADQSNRALINYENKKSKKIQWTIAGLMSLLFSINKVNAQ
                     QKTVKESPKSSWLAANRNEDMVTIKQSVTVLKDNAKNLNTPYLHQVFAIENSLVSKSA
                     SAFLINNNDTTIIKEVKDTSLSFFDKASFEQTNIMMGGITTFSDEVKPAPFTTFVKKV
                     FNKSFFKISPNPTNNNGVTISIKQPGNYVVQIFNNNSILVYTTEIEVSYKDEKVQIKF
                     NGSIAKGTYYIRVIDKKNNNQYTDKLLVN"
     gene            3268..3555
                     /locus_tag="PLU36_02575"
     CDS             3268..3555
                     /locus_tag="PLU36_02575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ45667.1"
                     /translation="MNKHIFKLFKKKKVSVFPNPATKGKVVNLMINKGNYAVQLEDKN
                     SQAVYEQEIMVANKNEVKLFNIPSNINNGTYYIKVVNKNNQQVLRSCLKVV"
     gene            complement(3560..4462)
                     /gene="xerD"
                     /locus_tag="PLU36_02580"
     CDS             complement(3560..4462)
                     /gene="xerD"
                     /locus_tag="PLU36_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HMZ45668.1"
                     /translation="MWQPYKKGYKDWLQLEKSLSDNSVEAYLRDIEKLTEYLIVQNSP
                     KKINEISLKDLQLFIQWIGELGMTPTSQARIVSGIRSFFKYCLTEQLITSDPSILLET
                     PKVKRALPDVLSFEEIENIINQIDLSKPDGGRNKAIVETMYSCGLRVSELINLKISCL
                     YLDVGFIRVIGKGDKERLVPIGSDAIKYITIYKNNIRTNIVPKQGFEDILFLNKFGKS
                     ISRVMIFYIIKELVLKANIDKNISPHTFRHSFATHLVEGGANLRAVQEMLGHESITTT
                     EIYTHLDREFLRTTLTQFHPAFKK"
     gene            4579..5082
                     /locus_tag="PLU36_02585"
     CDS             4579..5082
                     /locus_tag="PLU36_02585"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HMZ45669.1"
                     /translation="MQPVNLRSFKQKVFHHKKAFKKFLSKVEKNPPKNINSIANKTNE
                     AVWQEIDCLSCANCCKVMSPTFTTNDIKRISTHLKLTTQEFKDKYLFYDKKDGDWMNK
                     KQPCQFLNLTTNKCNIYEIRPEDCAGFPHLKKKKITDYIHVHQQNIEYCPATYKMVEK
                     WMIAINK"
     gene            complement(5164..6168)
                     /locus_tag="PLU36_02590"
     CDS             complement(5164..6168)
                     /locus_tag="PLU36_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45670.1"
                     /translation="MSYTQFPTSNSGSENKLTNSDSENKNNKNKIIYGLLILALIGSW
                     AYIIYEKNKNKEEKILFVDRIQKDSIDRVALQLQFDMLSSKADSLTTNNQELQGSLAE
                     RASEINKLKASIASILKKKNATAAELATAQKQIAELNNKIEELYAEVETLKAQNKQLT
                     ASNEQLNEEKKKLNEEKDALNSDLDKTKKDKAHVEDVASTLHANNINITAINLKGSKE
                     KETSKAKKADFFRISFDISENYITPSGKKALMVCVYYPDGSLSQSVGSFTDRQGNSKQ
                     YTNKMEIEYEQGRKMPVSFDWKPGNKFQTGLYKIEIYNNGFKIGEGAKKLDKSTFLGM
                     "
     gene            6291..7004
                     /locus_tag="PLU36_02595"
     CDS             6291..7004
                     /locus_tag="PLU36_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HMZ45671.1"
                     /translation="MLKDLLIIDIETVPIVESFNELSKEWQTLWWDKVSNSLPPNCTP
                     EESWNRKAGILAEFGKIICISTAYFYENENRNQCLKIKSIYGHNEVDVLNQFILLCSK
                     MYKHNKNFIYCGHNIKEFDIPYICRRLMINNLPIPEYLWLHDRKPWDVNMFDTLNWWK
                     FGDNKNYISLHLLASVLNIPTSKSDIDGSKVQEVYYKEKNLQRIVEYCQKDVVVCANI
                     VLKYKGIPLLSNESIFITN"
     gene            7137..7871
                     /locus_tag="PLU36_02600"
     CDS             7137..7871
                     /locus_tag="PLU36_02600"
                     /inference="COORDINATES: protein motif:HMM:NF023521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HMZ45672.1"
                     /translation="MKKIFLIIFLLPFLSNAQVLGGNNVIKANLSSLAFGNYHLTYER
                     ALLKKLSISVSYRYMPKKTVPLSNLLEKYIDNKDVNFGSFEMGNTAITPELRLYPVKK
                     LKGFYLAAYGRFADFDLSVPVRYVASSLPGSPTEYATFNGKIKSSSIGFMIGTQFRLA
                     KKLVLDWWIIGGHYGSSKGDLNALINRNLSPIEQQSLQATLDDLKEIGPFKFEGKVTS
                     PNTANVKSTGPWAGVRSMGLSLGVRF"
     gene            complement(7951..9630)
                     /locus_tag="PLU36_02605"
     CDS             complement(7951..9630)
                     /locus_tag="PLU36_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HMZ45673.1"
                     /translation="MTNSEIADNFSLLSKLMDINRENSFKAKSYSSAAFTIDKLPVQL
                     SSLEQNKIFSLKGIGESVGQKIIEQINNGFLQTLNEYILKTPIGVREMLNIKGIGPKK
                     IATIWHEMEIESIGELLYACNENRLTLFKGFGEKTQQTIKENIEFYLNTFGNYLYQQI
                     ENYAIAFHQKLQQTFLHSEFIFTGSFRRQIEVMDKVEWVTNVEVNELKIFFKSNNYNI
                     IQENEGYIIFKGIENVELCFYLTDVSFLFNQLFATSCSNHFLEEWISKTGWQITDPYE
                     SEEEIFSLNNCNYIPAYQRETAEIIALAFKQPLPKVIETRDIKGIIHSHSNWSDGVNT
                     IEEMANACMLKGFEYLVISDHSKTAFYAQGLSVERVLAQHILIDELNKKLYPFKIFKS
                     IESDILNDGSLDYEPEILNKFDLVIASIHSNLKMNEEKAMHRILGAVTNPYTSILGHL
                     TGRLLLSRNGYPVNYSTLFEACKKHRVVIELNAHPRRLDMDWRYINDAVDNNILISIN
                     PDAHAIEGFGDCKYGVLIAQKAGLTTNNNLSSFSLQQMNTFIETQKLKRRG"
     gene            9767..10567
                     /locus_tag="PLU36_02610"
     CDS             9767..10567
                     /locus_tag="PLU36_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45674.1"
                     /translation="MKIKLLISLVIIQFTVIKSVNAQEINSVTFLKEFNELFSDAQDG
                     FPKSKGSLLEDIGWFGRKYAANKYFFNNSAMAALQYIKPHEPTKYVPKATPEQFYFTQ
                     TFIVERNEGKFANDSLEIVLDLIAKGGGYDKKNIKQEKEYRKTYKSIEYQKSSKPIYS
                     LLKNLKSNTYSITIYSPVRPSDVAKPKNRLGCIVFKYTGFSYVYAVTVYGEKLSNVNN
                     IVEKAFKATGLVDRYNSYFWHPKKSVDQISIELGNSVIVRDAGSIDVD"
     gene            complement(10665..14471)
                     /gene="rpoB"
                     /locus_tag="PLU36_02615"
     CDS             complement(10665..14471)
                     /gene="rpoB"
                     /locus_tag="PLU36_02615"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HMZ45675.1"
                     /translation="MSTTTLNNRINFGKTKHLAETPDLLDIQLESFKEFFQLETTPDK
                     RNNEGLFRVFKDNFPITDTRNIFVLEFLDYFIDPPRYSIAECMERGLTYAVPLKAKLR
                     LSCNDEEHVDFQTIVQDVFLGNIPYMTPRGTFVINGAERVVVSQLHRSPGVFFGQSVH
                     PNGTKIYSARVIPFKGAWMEFATDINNIMYAYIDRKKKFPVTTLLRSIGFETDKDILE
                     LFGMADEVLTDKKELSKHIGKRLAARVLRTWIEDFVDEDTGEVVSIERNEIVMERDTV
                     LDEDGINTIIEMQVKSVFIQKEDVGGDYAIIYNTLNKDTSNSELEAVQHIYRQLRGAD
                     APDNETARGIIDKLFFSDKRYDLGEVGRYKINRKLGLDYPLTKKVLTKQDIIEIIKYL
                     VRLTNSKAEIDDIDHLSNRRVRTVGEQLYAQFGVGLARMARTIRERMNVRDNEVFTPV
                     DLINARTLSSVINSFFGTSQLSQFLDQTNPLSEITHKRRISALGPGGLSRERAGFEVR
                     DVHYSHYGRLCTIETPEGPNIGLISTLCVHASINEMGFIETPYRKVTNGKVNMKELTF
                     LSAEEEDTAKIAQSSTPLDNSGNFIEDRIVSRETGDFPILDKSDVEYIDVAPNQIVGL
                     SASLIPFLEHDDANRALMGSNMQRQAVPLIRPEVPVVGTGLEGKAARDARIQIHADGN
                     GVVEFVDSREIHVRYDRNEGERLISFEDDLVVYQLTKFIKTNQATCINLRPAVKKGQK
                     VSAGDFLTEGYATQNGELALGRNLQVAFMPWKGYNFEDAIVINERVVREDWFTSVHIE
                     EYELEVRDTKLGEEELTPDIPNVSEDATKDLDENGIIRIGATIKEGDILIGKITPKGE
                     SDPTPEEKLLRAIFGDKAGDAKDASLKAPNGTEGVVIDKKLFQRAKKDKNAKVREKAL
                     LDKVEKLHQDNEEDLKEQLLNKLQILLRDKSSLGVSNSFGEVLIGKGAKFNQKNLATI
                     DYATVNPLGWTGDEKTDDSINTLLHNYTIKYNEELGRYKREKFNISIGDELPAGVLKL
                     AKVYLAVKRKLKVGDKMAGRHGNKGIVAKIVRAEDMPFMEDGTPVDIVLNPLGVPSRM
                     NLGQIYETILGWSAKKLGVRFATPIFDGASTTEIEEYCNQAAIPLNGHTYLFDGETGD
                     RFHQKATVGVIYMIKLHHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWA
                     LEAYGASNILQELLTIKSDDIIGRAKTYEAIVKGDNIPKAGVPESFNVLVHELRGLGL
                     DLNFEQ"
BASE COUNT         5041 a         2354 c         2038 g         5416 t
ORIGIN      
        1 aaaataaggc tgtctatctt tttgatttca ctattttcaa tataattctg tgccacaaca
       61 ctatggcagt aaaaaaataa acttaataaa aatgaatact tcaatgccat aataaatagc
      121 aaatatcaaa aataacactt ctgcaattta tataacaata gagggataga agtaagtttt
      181 taggggcaga aagaatgttg agaataattt tacaacttaa ttataacaac agccttatta
      241 ttcgtagtat tttttacttg ccaattaacg ccattttcaa ttaattcaat agctcttaca
      301 gatttttctg gaatagagct ttgagcttga atttgataag gcttgccttc tttatcaatt
      361 aaaaaagtaa caattacaga ttctactagt tcgtttgtaa aatgtaatgc tgctcttttt
      421 gtattaatat aatcatcaaa ttttttccaa ccaccaatag gagtaattcc tttattgttc
      481 tgttcattat ctgatatttt tagaacagca gcagttgctt tcttactttt tgatgcttca
      541 gctaaattag aagcaatagc attcctttct tctatatttc tagaattttg tgcatttaca
      601 gcaccattat ttggaaatgc aggaggctct cctaccatgc ttttaacaga aatatctttg
      661 ggtttatttt tatttaaagc atcttcagta ctaaaaattc ctgcacttga ttcggtagtt
      721 tttattgcag cttgtggcga actaattaat gcattatcat taattatatt aaagtcttca
      781 atttttacct tctctttttt attggatgca ttttgctgat gattatttac tattttttca
      841 gaagtatttt ctttttctgt aggtaatgca gaaatagtat ttggagtttg ttttatttct
      901 tctttggtaa ttgctgctaa agtattttct tttttagtac ttgtattatt atttaaaagc
      961 catattgtag ctcctgcacc aatcattatt ataaaacctg cggcaattct taaccattgc
     1021 tttttatata ttggcatagt taccactttt gcttttccaa cttctttact taatatagtt
     1081 gttgctgttt cgccaatata ttttttagct tcggttaggt ttgcattttc aaaaccttct
     1141 aaagcatctg ctaaaaaggg atctgctaat gttgcttttt caaatgcatt cctttctgca
     1201 ttacccatat tgttttgtaa ataattttca atatcggcta aattgtaatt tttattttgt
     1261 ttaagtgcag aagctgaaat gaggttatct gtttctttca acatttgaag tgctttagta
     1321 gtttcagcaa tttcaaaacc ttctaaagca tctgctaaaa agggatctgc taatgctgct
     1381 ttttcaaaag catttctttc agcattgctc attttgcctt gcaaatagtt attaatatct
     1441 gctgctgtat aattaatatg gtgtgtaaaa tcagacattt ttatccatgc aaattttcag
     1501 gttacgcctt ccgttttgta ttaaactacg tactttattc cattctattc cagtaatatc
     1561 tgctatttcg ttatagcatt tattttctaa ataaaacaac tgaatacttt tcttttgttc
     1621 ttcatttaaa gtttcaatac aaccttccag gctatttaat ttttgttctt tttctaaaac
     1681 attatcaaga tgcgaaaagt cttcggattg cataaattgt ccatcaaatt ctactgttgt
     1741 tgttttttta ttctttctta actgcattaa acagtggttt tttgttacca catataacca
     1801 acttttaaaa ttatccacct tatgattagg aagcttttga actaattcct ggtaaatatc
     1861 cattacagca tcacggcttg tttcttcatc tttaaaatat ttcatacaag taccaaacac
     1921 taaatcatta tacctgagaa ataaagtagc caacgatttt tgatctaagc tttgctggta
     1981 caaaagcatt aaagaattat cgtctgctgt attatcaata tgttttacag ttaatgccat
     2041 aaggcactaa gataaatatt taatgatata tacaaataaa ctgttataaa aattgaaata
     2101 attctaaaga aataaacaat ttacccattg tagttctatt tattaaaaaa gtatgtaatt
     2161 gttcaaaagg caacatctgt aactaaacaa attatatgaa atcagctatt tttttgcaga
     2221 ttgctacacc ctgtaacgag aattgggaaa agatgacacc tatggaaaaa ggtaaatttt
     2281 gtgctagttg tgcaatgcag gttacagatt ttacattaat gactgataaa gaaatactgc
     2341 aatacttatc tactacacga ggtaaggttt gtggaagaat tcatgctgac caatctaaca
     2401 gggctttaat aaattatgaa aataaaaaaa gtaaaaaaat acaatggacc attgcaggtt
     2461 taatgtctct gttattttca atcaataaag taaatgctca acaaaaaact gtaaaggaat
     2521 ctccaaaatc atcttggctt gccgccaata gaaatgaaga tatggtaact attaaacaat
     2581 cagtaactgt attaaaagat aatgctaaaa atttaaacac tccatactta catcaggtat
     2641 ttgctatcga aaatagttta gttagcaaat cagcctctgc atttttaatt aataataatg
     2701 ataccacaat cattaaagaa gtaaaagaca cttcgttatc attttttgat aaagcaagtt
     2761 ttgaacaaac caatattatg atgggtggaa taacaacctt ttctgatgaa gtaaaacctg
     2821 caccatttac aactttcgta aaaaaagtat ttaataaatc ttttttcaaa atttcaccaa
     2881 accctactaa taataatggc gtaactattt ctattaagca accaggcaac tatgtagtac
     2941 aaatttttaa taacaattca atactagttt acacaacaga aattgaagta agctacaagg
     3001 atgaaaaagt gcaaattaaa tttaatggca gtattgctaa aggaacatat tatataagag
     3061 ttattgacaa aaaaaataat aatcaatata ccgataaact tttagtgaat taaaaatatt
     3121 aatggttaca agtattatta tctatatcca ttaatgaatg gtgagcaaca aaattttctt
     3181 atattatgta attattaaat tctcttcatc tgtatttata attcaatcat cagtacaaaa
     3241 tttgcataat taaaatatat tttttttatg aacaagcata tttttaaact atttaaaaaa
     3301 aagaaagttt ctgtttttcc taatcctgca acaaaaggca aggttgtaaa tttaatgatt
     3361 aataaaggca attatgctgt acaattagaa gataagaata gccaggctgt atatgagcaa
     3421 gagattatgg ttgcaaacaa aaacgaggtg aaactattta atattcccag taatattaac
     3481 aatggcactt attacattaa agtagtcaat aaaaataatc agcaagtttt aagaagttgt
     3541 ttgaaagtag tttaataatt tattttttaa aggctggatg aaactgtgtt aatgtagttc
     3601 tcaaaaattc tctgtctaaa tgtgtgtaaa tttcagtggt tgtaatactt tcatgaccta
     3661 gcatttcttg cactgctctt aaatttgctc ctccctctac caaatgtgtt gcaaaagaat
     3721 gacgaaaagt atgaggtgaa atatttttat caatattagc ttttaatact aactctttaa
     3781 tgatataaaa aatcattaca cgagaaatag atttaccaaa cttgtttaaa aacaaaatat
     3841 cctcaaaccc ttgctttggc actatattag ttcttatatt gtttttataa attgtaatat
     3901 attttatagc atcgctacca attggcacca gtctttcctt atcgcctttt cctattactc
     3961 taataaagcc aacatctaaa tacaaacaac ttattttaag gtttattaat tcgctcactc
     4021 ttaaaccgca gctatacata gtttctacaa tggctttatt tctaccacca tcaggtttac
     4081 ttaaatcaat ttgattgata atattttcaa tctcctcaaa gcttaacaca tctggcaaag
     4141 ctctttttac ttttggtgtt tctaataaaa tagatggatc acttgtaata agttgttcgg
     4201 ttaggcaata cttaaaaaaa cttcttatgc cagaaactat acgagcttgc gaagttggtg
     4261 tcataccaag ctctcctatc cactgaataa aaagttgcaa atcttttaaa ctaatttcat
     4321 ttattttttt gggactgttt tgtacaatta aatattccgt aagcttctct atatctctta
     4381 aataagcttc tacagaatta tcgcttaaag atttttctaa ctgtaaccaa tctttatatc
     4441 cttttttgta tggctgccac attattaaag aagtaagtta taaaagtagg agatgttttt
     4501 tatttgaaag tacaattatg taactatatt cgtttttaaa acaagtacga aaatttattt
     4561 tcaattatta aatttacaat gcaaccagtt aatttacgtt cgtttaaaca aaaagttttt
     4621 catcataaaa aagcttttaa aaaattttta agtaaagtag aaaaaaatcc tcctaaaaat
     4681 attaatagta ttgcaaacaa aactaatgaa gcagtttggc aagaaataga ttgtttaagt
     4741 tgtgctaatt gctgtaaagt aatgagccct acatttacta caaatgatat taaaagaatt
     4801 tctacccatt taaaattaac cactcaagaa tttaaagaca agtatttgtt ttatgataaa
     4861 aaagatggcg attggatgaa taaaaaacaa ccttgccagt ttttaaattt aacaacaaat
     4921 aaatgcaata tttatgaaat taggccagaa gattgtgccg gatttccgca tttaaaaaag
     4981 aaaaaaataa ccgattatat tcacgttcat caacaaaata ttgagtactg ccctgcaacc
     5041 tataaaatgg tagaaaaatg gatgattgct ataaacaagt agaagcagtt agtgttataa
     5101 aaaaaggcta cccaacttca ggtagccatt ttttttataa gatgaaaatt aaaatttata
     5161 caactacatt cctaaaaaag tagatttatc caatttttta gctccctcac caattttaaa
     5221 accattgtta taaatttcaa ttttatatag tcctgtttga aatttattac caggtttcca
     5281 atcaaagctt actggcattt ttcttccttg ctcatactca atttccattt tattagtgta
     5341 ttgttttgaa tttccttgcc tatcagtaaa agaacctact gattgtgaca aagatccatc
     5401 tgggtaataa acacaaacca ttaatgcctt tttgccactg ggtgtaatat agttttcaga
     5461 aatatcaaaa cttattctaa agaaatcagc tttttttgcc ttacttgttt ctttctcctt
     5521 actacctttt aaattaattg ctgtaatatt aatattgttt gcatgtaaag tactagcaac
     5581 atcttcaaca tgtgctttat cttttttagt tttatctaaa tcagaattca aagcatcttt
     5641 ttcttcgttt agttttttct tttcttcatt tagttgttcg ttgctggcag taagctgttt
     5701 gttttgtgct tttaaagttt ccacctcagc atacaattct tcaattttat tatttaactc
     5761 tgcaatttgc ttttgtgcag tggctaactc tgcagctgtg gcatttttct tttttagaat
     5821 agaggcgatg cttgctttta atttattaat ttctgatgcc ctttctgcta atgagccttg
     5881 caactcctgg ttattagttg ttaaagaatc tgctttacta cttaacatat caaattgtaa
     5941 ttgtagtgca actctatcaa ttgaatcctt ctgaattcta tctacaaata aaattttttc
     6001 ttctttattt ttattttttt cgtaaataat atatgcccaa ctgccaatta atgcaagaat
     6061 tagcaaccca taaataattt tatttttatt gttcttgttt tcagaatcag aattagttaa
     6121 cttattttca gatcctgaat tagatgttgg aaattgtgtg taactcatat tttattatta
     6181 ctttaaaatg tgctgcaata tagttttctt tttatttaaa aaatgtgaaa atgtttaatc
     6241 caattgtaat tgtaaattat cctgtaactt taaaaggcta aattgtagat atgctaaaag
     6301 atttattaat catagatatt gaaacagtac ctattgttga aagctttaat gagctatcta
     6361 aagaatggca aacactatgg tgggataagg tttctaattc gttaccacca aattgcacac
     6421 ccgaagaaag ctggaataga aaagcaggta ttttagcaga gtttggaaaa attatttgta
     6481 taagtacagc ttatttttat gaaaatgaaa atagaaatca gtgtttaaaa ataaaaagta
     6541 tttatggcca caatgaggta gatgttttaa accaatttat tttattgtgt agcaaaatgt
     6601 ataagcataa taaaaatttt atttactgtg gtcataacat taaagaattt gatatacctt
     6661 atatatgccg caggttaatg attaataact tacccatacc cgaatatctt tggctacatg
     6721 atagaaagcc ttgggatgta aatatgtttg acactttaaa ttggtggaaa tttggcgata
     6781 ataaaaacta tatatcctta cacttattag cttctgtttt aaatattcct acaagtaaaa
     6841 gcgatataga tggtagtaaa gtgcaagaag tttattacaa agaaaaaaac ttacaacgaa
     6901 ttgttgagta ctgccaaaaa gatgttgtag tttgtgctaa tatagtttta aagtacaagg
     6961 gcataccact gcttagtaat gaaagtatat ttattacaaa ttagaacata aaataccatt
     7021 aattgggtat ttggcttatt gaaaaaaaaa ttatcattgc aattaataat cgttaacttt
     7081 atttataaat tattataaat aaccatttaa atacaaaata atttaaaatt taaaccatga
     7141 aaaagatatt cttaattatt tttcttttac cgtttttatc taatgcacaa gttttaggtg
     7201 gcaacaatgt tattaaagcc aacttatctt ctttagcttt tggcaattac catttaactt
     7261 acgaaagagc attacttaaa aaactttcta tctctgtttc ttacagatat atgcctaaaa
     7321 aaactgttcc tttatctaat ttattagaaa aatatataga caataaggat gttaactttg
     7381 gaagttttga aatgggtaat actgctatta ctccagagtt aagactatat cctgttaaga
     7441 aattaaaagg attttattta gctgcctatg gcagatttgc agattttgat ttaagtgttc
     7501 ctgtaagata tgttgctagc tcattaccag gcagcccaac agaatatgct acttttaacg
     7561 gaaaaattaa atcgtcaagt attggtttta tgattggtac acaatttagg cttgctaaaa
     7621 aattagtact agactggtgg attataggtg gccactatgg cagcagcaaa ggagatttga
     7681 atgctttaat taacagaaat ctttctccta tagagcaaca aagccttcaa gctaccttag
     7741 atgacttaaa agaaattggc ccttttaaat ttgaaggtaa agtaacctcg ccaaatacag
     7801 ctaatgtaaa atctacaggc ccatgggcag gtgttagaag tatgggttta agtttaggtg
     7861 ttaggtttta aataaataaa atttcataat aaaataaggc agcccaaggg ctgccttatt
     7921 ttattatgac taatatagcc tctaaaattt ttatcctcta cgttttaact tttgagtctc
     7981 tataaaagtg ttcatttgct gtaaactaaa gctacttaaa ttattattag ttgttagccc
     8041 tgctttttgt gcaattagca caccatattt acaatctcca aacccttcaa tagcatgagc
     8101 atcagggttt atagaaatta aaatattatt atctactgca tcattgatgt atcgccaatc
     8161 catatctaat cttctagggt gagcatttag ttctattact acccggtgtt ttttacatgc
     8221 ttcaaataaa gtagaataat tcaccggata tccatttcta cttaataata acctacctgt
     8281 taaatgtcct aaaatagaag tatatggatt ggtaacagca cctaaaatac gatgcattgc
     8341 tttttcttca ttcattttta aattagaatg tatagaagct attaccaaat cgaatttatt
     8401 taaaatttct ggctcataat ctaagctgcc atcatttaaa atatcgcttt ctatactttt
     8461 aaatatttta aagggataaa gttttttatt taattcatct atcaaaatat gttgtgctaa
     8521 aactctttct acgcttaacc cttgagcata aaaagctgtt ttgctatggt cgctaataac
     8581 taaatattca aagcctttta gcatacaggc attagccatt tcttcaatag tatttacacc
     8641 atcactccaa ttactatggc tatgaatgat gccttttata tctcttgttt ctattacttt
     8701 gggtaatggc tgcttaaagg ctaatgcaat tatttctgca gtttctcttt ggtaagctgg
     8761 tatataattg caattgttta aagaaaaaat ttcttcttct gattcataag ggtccgttat
     8821 ttgccagcct gttttactta tccattcttc taaaaaatga ttactgcaac ttgttgcaaa
     8881 aagttggtta aataaaaacg aaacatcggt taaataaaaa caaagttcta cattttcaat
     8941 tcctttaaaa attatatagc cctcattttc ctgaattatg ttataattat ttgacttaaa
     9001 aaaaattttc aactcattaa cttccacatt ggttacccat tctactttat ccattacttc
     9061 tatttgtctt ctaaaagaac ctgtaaatat aaattcggaa tgtaaaaatg tttgttgcaa
     9121 tttttgatga aatgcaattg cataattctc tatttgttga tacagataat tgccaaaagt
     9181 gtttaagtaa aactcaatat tttcttttat ggtttgttgt gttttttcgc caaaaccttt
     9241 aaataaagtc aatctatttt cattacaagc ataaagcaat tcgccaatac tttcaatttc
     9301 catttcatgc catattgtag caattttttt ggggccaatg ccttttatat taagcatttc
     9361 tcttacccca atgggtgttt ttaaaatata ctcattaaga gtttgtaaaa agccgttatt
     9421 tatttgttct ataatttttt gtccaacaga ttcgccaatt ccttttaaag aaaaaatttt
     9481 attttgctct aaagaagaaa gttgtacagg tagtttatca atagtaaaag cagccgagga
     9541 atatgacttt gctttaaaac tattctccct attaatatcc attagtttag atagtaaaga
     9601 aaagttatca gctatttccg aattggtcat tttaataaat gtttgtgttg aacaaattaa
     9661 gcatttttaa aaaaatattt caaacaataa tttataaaat gtaacatgta tttattttta
     9721 ctgaataaaa gaataatata ttgttatacc tttttaaata ttaattatga aaataaagtt
     9781 attaattagt ttagtgataa ttcagtttac agttatcaaa agtgtaaatg ctcaagaaat
     9841 aaattctgtt acctttttaa aagaatttaa tgaacttttt tctgatgcac aggatggttt
     9901 tcctaaaagt aaaggtagtt tgttagaaga tattggttgg tttggtagaa aatatgcggc
     9961 caataaatat ttttttaata attctgctat ggcagcatta caatatatta aaccacacga
    10021 acctacaaag tatgttccta aagcaacacc agagcagttt tattttacac aaacatttat
    10081 tgtagaaaga aatgaaggaa aatttgccaa cgattcttta gaaatagttt tagatttaat
    10141 agcaaaagga ggaggatatg ataaaaaaaa tattaagcaa gagaaagagt atagaaaaac
    10201 atacaaatca atagaatatc aaaaaagcag taaaccaata tactctttgc taaaaaatct
    10261 taaaagcaat acatattcca tcactattta ttctcctgtt agacccagtg atgttgcaaa
    10321 gcccaaaaac agattaggct gtattgtgtt taaatataca ggattttcat acgtatatgc
    10381 agttacagta tatggagaaa aactaagtaa tgtaaataat atagttgaaa aagcttttaa
    10441 agcaacaggg ctagtggata gatacaactc ctatttttgg catcccaaaa aatcagtaga
    10501 tcagatttct attgaattag gtaatagtgt tattgttaga gatgcaggaa gtattgatgt
    10561 ggattaaaaa atctttctta tatagcttat attttcttaa acaaaataaa aaaactccta
    10621 tattttttta tataggagtt tttaaatatt gaattaaaat ttatttactg ctcaaaattt
    10681 aaatctaagc ctaaacctct taattcatgt actaatacat taaaagattc tggtactcca
    10741 gcttttggaa tattatcgcc ttttacaata gcttcataag tttttgcacg gccaattata
    10801 tcatcagatt taatagttag taattcttgt aaaatattac ttgcaccgta agcttctaaa
    10861 gcccacactt ccatttcacc aaaacgctga ccaccaaatt gtgctttacc acctaaaggt
    10921 tgttgtgtaa ttaaactata agggccaatt gaacgagcat gcattttatc gtcaaccatg
    10981 tggtgtaact taatcatata aataacacca acagttgctt tttggtggaa gcgatcgcct
    11041 gtttcaccat caaataaata agtatgtccg tttaaaggaa ttgcagcttg attacaatat
    11101 tcttcaattt ccgttgtaga ggctccatca aaaattggtg tggcaaaacg cactcctaat
    11161 tttttagcac tccatcctaa aatagtttca taaatctgac caaggttcat acgagaaggt
    11221 acacctaaag ggtttagaac aatatctact ggtgttccat cttccataaa aggcatatct
    11281 tcagcacgta caattttggc tacaatacct ttgttaccat gtcggcctgc cattttatct
    11341 cctactttta atttacgttt tacagctaag taaactttgg ctaattttaa tacacctgca
    11401 ggtaattcgt ctccaattga aatattaaac ttctctcttt tgtatctacc taattcttcg
    11461 ttgtatttaa tagtgtagtt atgcagtaag gtattgatag agtcatctgt tttttcatcg
    11521 cctgtccaac ctaaaggatt aactgttgca taatctatag ttgctaaatt cttttgatta
    11581 aatttagccc ctttgccaat taaaacttct ccaaaactat tgcttacacc taatgaagat
    11641 ttatctctta ataagatttg caatttgttt aataattgct cttttaaatc ttcttcatta
    11701 tcctgatgta gtttttctac tttatctaat aatgcttttt cacgaacctt agcattttta
    11761 tctttcttag ctctttggaa aagcttttta tctattacta caccttctgt tccatttgga
    11821 gcttttaaag aagcatcttt tgcatcgcct gctttatctc caaaaatggc tcttaataat
    11881 ttttcttcag gggttgggtc actctctcct ttaggagtaa ttttaccaat taatatatcg
    11941 ccttctttaa tggttgcacc aatacgtata ataccatttt catctaaatc ttttgttgca
    12001 tcttcactaa cgtttggtat atctggtgtt aattcttctt ctcctaattt agtatctcta
    12061 acttctaatt catattcttc aatatgaaca gatgtaaacc aatcttcacg aacaactctt
    12121 tcattaatta caatagcatc ttcaaagttg tatcctttcc aaggcataaa agcaacctgt
    12181 aagtttctac ctaaagctaa ttcaccattt tgggtagcat atccttctgt taagaaatct
    12241 cctgcactaa ctttttgacc ttttttaact gctggtctta aattaataca agtagcttgg
    12301 tttgttttaa tgaatttagt tagttgataa accactaaat catcttcaaa acttataagt
    12361 ctttcgcctt catttctgtc ataacgaaca tgaatttctc ttgaatcaac aaattctaca
    12421 accccatttc cgtctgcatg aatttgaatt ctggcatccc gtgctgcctt accttctaag
    12481 ccggtaccta caactggtac ttcaggacga atcaaaggaa ccgcttgacg ttgcatgttg
    12541 gaacccatta aggcacggtt tgcatcatca tgttctaaga aaggaattaa agaagcactt
    12601 aagcctacaa tttggttagg tgcaacatct atatattcta catcagactt atcaagtata
    12661 ggaaaatcgc cagtttcgcg gcttacaatt ctatcttcaa taaagtttcc actattatct
    12721 aaaggcgtac tactttgggc aattttagct gtatcttctt cttcggcact taaaaaagtt
    12781 aattctttca tattaacttt tccgttggtt actttacgat aaggcgtttc aataaagccc
    12841 atttcattaa tacttgcatg tacacataag gtagaaatta acccaatgtt tggcccctct
    12901 ggtgtttcaa tagtacataa acgaccataa tgtgaatagt gtacgtcacg aacttcaaaa
    12961 cctgcacgtt ctctgcttaa accacctggc cctaaagctg aaatacgacg tttgtgtgta
    13021 atttctgata aaggattggt ttgatctaag aattgtgata attgtgaagt accaaagaaa
    13081 gaattaatta cagaagaaag tgttctggca ttaatcaaat ctactggtgt aaatacttca
    13141 ttatctcgta cattcattct ttctctaatg gtacgtgcca tacgagctaa gccaactcca
    13201 aattgtgcat acaattgttc gccaactgta cgtacacgac gattggataa atgatctata
    13261 tcatctattt cggctttaga attagttaaa cgaactaaat attttattat ttctatgata
    13321 tcttgcttag ttaaaacctt tttagttaaa ggataatcta aaccaagttt tctattaatt
    13381 ttataacgtc ctacttctcc taaatcataa cgtttgtccg agaagaataa tttatctatt
    13441 ataccacgtg ctgtttcatt atctggagca tcggcaccac gtaattgacg atagatatgt
    13501 tgcacagctt ctaattcgct gttagaagta tctttattta aagtgttgta aataattgca
    13561 taatcgccac caacatcttc tttttgtata aatacgcttt taacctgcat ttcaataatg
    13621 gtattaatac catcttcgtc taatacagta tccctttcca ttactatttc atttctctca
    13681 atactcacta cctcaccagt atcttcatcc acaaaatctt caatccaagt acgtagtacc
    13741 ctagctgcaa gccttttacc aatatgttta gataattctt ttttatcggt cagtacttca
    13801 tcagccatac caaataattc aagaatatct ttatctgttt cgaagccaat ggaacgtaat
    13861 aaagtagtta cagggaactt ctttttacga tctatataag catacataat attgttaata
    13921 tctgttgcaa actccatcca agcaccttta aaaggaatta ctcttgcaga gtaaatttta
    13981 gtgccattag ggtgaacaga ttgcccaaag aatacaccag gagaacgatg taattgtgaa
    14041 accactacac gttctgcacc atttataaca aaggtacctc ttggtgtcat ataaggtata
    14101 ttacctaaaa acacatcttg aacaatggtt tgaaaatcta cgtgttcttc atcgttacaa
    14161 cttaaacgta atttggcttt taaaggaaca gcgtaagtta acccacgttc catacattct
    14221 gcaatagaat agcgtggtgg gtcaataaaa taatctaaaa attctaatac aaatatgttt
    14281 ctagtatcag ttattggaaa attgtcttta aatacacgaa ataagccttc gttattacgc
    14341 ttatctgggg ttgtttctaa ctggaaaaat tctttaaaag actctaactg aatgtctaaa
    14401 aggtcaggag tttcggcaag atgttttgtt ttaccaaaat taatcctgtt gttcaatgta
    14461 gttgttgaca taatattttg taaaaccggt ttattgcaat aataatttgg aaaaaagcat
    14521 atactctttt ataaactgat attcagacaa ttatgcttaa atggccatta acataatcat
    14581 caaaatatag tttgccaaat ttaaaaggat tgcaaaagta cgtttttgat tgggaatgac
    14641 aaaactattt ttttttaaaa gatttatata atttattttt aaaaataaaa gaagtttaaa
    14701 aggcgacata aataatgcaa gttttcaaaa taaaagcact gaaaatcaat acaaaatatt
    14761 tttgttgaat atgaaaaggt tcgtatattt gagagttagc ggtcattgta gaaaaacgca
    14821 cccctaaaaa tgactgaata aaaaagata
//
LOCUS       DAOQPK010000030        14832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_2059_length_14832_cov_4.747378, whole genome shotgun sequence.
ACCESSION   DAOQPK010000030 DAOQPK010000000
VERSION     DAOQPK010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 14832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14832
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2059_length_14832_cov_4.747378"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..1172
                     /locus_tag="PLU36_02620"
     CDS             15..1172
                     /locus_tag="PLU36_02620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ45676.1"
                     /translation="MPKKKILLLYEYFYPGFKAGGPIQSLVNLLLAMQHQYEFYVATT
                     AYDMQTATPYPNVVCNKWVNTQLTPNALPVKVWYSSNIKPGYKELLQTILTSGCTTIY
                     INGFYTNYFLFPLLYLKLGKLKNMDCIVAPRGMLTKGALSVKPFRKKIYFTVIKCFGL
                     LKTVRYHATNTEEVQEIQSMFGRSSSITLASNVSKPPFEKFRQSTKNNDELRLVFLAL
                     ISPMKNLLLLLQYLQSCSNTIVLDIYGPVKDKAYWQQCLQAIQALPKNIKVHYKGDAE
                     PHTVQNIFTGYDAMILLTKGENFGHAIYESLSVGTPVIISHFTPWNNLQEKKAGWNIN
                     INNKEELLTTLHQLSKMTLQEHNDCSNNAWHVAKDYYEQSNYTKNYNNLFS"
     gene            1178..2902
                     /locus_tag="PLU36_02625"
     CDS             1178..2902
                     /locus_tag="PLU36_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HMZ45677.1"
                     /translation="MIILGINAYHADSSAAIFKDGIMIAATEEERFRRIKHWAGFPSM
                     AIEFCLKEVGVTLKEVDHIAIGRDPKAKFLKKLFFFAQHPVHSIKFVAERWSNQKQVT
                     SIEKELAQLSGLDETEIKNKIHQVEHHRSHLASAFFASPFEEAALLSIDGSGDFSTTM
                     LGIGKGNKIEVIDSVDFPHSMGIFYTAFTQLLGFPYYGDEYKVMGLAPYGEPKYVDKL
                     RDVVLWKDNGLFQLNFAYFRSATQGFIKYENNLPIVPPLFGKKMVEVFGAERKKDEPL
                     SQYHKDVAASVQRFTEETIFHILKVLHKRTGLDKVCIAGGVAQNSVANGKLTRNTPFK
                     EVYIPSAGHDAGISMGAAQYVYHQIFNKPRVAPIYSAYTGAKFSNDEIEVLLKTKNIN
                     YKKYDDETLYNVVADRLINAGVVGWFNGRAEFGPRALGARSILADPRRNDAKDLLNAK
                     IKRRESFRPFAPSILKEFVEEYFEVNDIVPFMEKVFPIKKEKQHLIPAVTHVDGTGRL
                     QSVDSNTSPAYHSLIKNFYNKTGVPILLNTSFNENEPIVNSPLEALDCFLRTSMDMLV
                     LENIVIER"
     gene            2911..3666
                     /locus_tag="PLU36_02630"
     CDS             2911..3666
                     /locus_tag="PLU36_02630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ45678.1"
                     /translation="MKVSIITATYNSATTVADTLLSIEKQTYLPNIEHIIVDGLSADN
                     TLEIVNHFQHVTKVISEKDKGIYDAMNKGIALANGDIIGILNSDDFYAHDNVIHRVVE
                     IFNTTNCDAVYGDLIFVHPNNPNKVLRKWIAGSFNANMFLKGWMPPHPTFFVRKEVYE
                     KYGKFNLTLKSSADYELLLRFLYKHTIKVEYLRDVIVKMRSGGQSTKSIANRIRAHKE
                     DYMAWKINNLKPKWFTLLFKPARKIKQFLYPRI"
     gene            3712..6189
                     /locus_tag="PLU36_02635"
     CDS             3712..6189
                     /locus_tag="PLU36_02635"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HMZ45679.1"
                     /translation="MKVFLFTIRVFLLIVCISFVSNVAAQSGSLQELINSRDLSNFKV
                     EMLKDEDIIKYKTYLQNAGITESQAEQMALQRGMPSSEILKLKARVASLSSRTTTTTK
                     PTTKGRTNTSRDIDSLDVEGSRVVEKEKVLKPEDEIFGSALFNNNDLRFEPDLRIATP
                     KNYIIGPDDEIAIDVYGYQETNPRLTVSPEGFINIPNVGFVSVNGLTVETAAKRIKEK
                     MIKNGYSSAATGNTKIEVSISKIRTIRVTVIGEAKKPGTYNVSSLSSLFNVLYAAGGP
                     SNKGSFRTIELIRNNKVIATLDAYDFLLKGIQQNNVKLTDQDVVRIPAANILVKLKGE
                     VKHQGIFEMLPTENMNSLLSFAQGFTGKAYTASVNVKSFTDIERKIQDINKTEFINYK
                     PKNGDEVFVGRVLERYSNRVILEGAVYRPGQYELTSNLTLSKLIAKADGLKEDAYKER
                     GLLYRTNEDLTKQVIDFNLNDVNSNNNQDIILQKDDSIVIASAKDFYETYTLTVDGEV
                     RKPGVYEYYKGITLKGLIFQTGGFTDAATAQRIEIARRIKSDDINATAIAQVIEIATE
                     KDLSIKGEEVKLQPWDVVIVRSNPGYKPQIAVKVEGEVLYPGTYILGSKEDKVSDIIK
                     RAGGVTPHSDKTGAFITRINTSAFKDEAAERLQKLKKASDTSTQLIEELTKPTVKISL
                     RLDEVLNNKGNLLENITLMEGDVIHVPKQRNVVKVNGEVMFPTEIVYKEGASIHYYID
                     KAGGFTENARKKKVTVLNSNGSAAKTKKFLFFRSYPSVLPGSEILVPKIPVKENKGLS
                     TAEWLAVASGLASLAGVAVAIINVSRK"
     gene            6216..7268
                     /locus_tag="PLU36_02640"
     CDS             6216..7268
                     /locus_tag="PLU36_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45680.1"
                     /translation="MSLVNPEEKVVKAKHLIALMKYWWKFLYAKKVLIIAITFLGAVV
                     GFTFGWFQKPNYKAELTFAPEDAGQLGGYLGIAAQFGIDLGMGSGNVFEGENITELFK
                     SSTLISNTLYSEATIGGKNQTLIAYKIASKYKKNKPVLDSLQLHQVNFNQSLQNKALG
                     IRFADSLVKAIIKETSKSLDVNKVDKKLNIIKVSFTDKDEVFAKLFVDLLAQNAIKYY
                     TGYKSKKARDNVEILQHQADSVKRMLFGGIGDVASMNDLNVNPTKQTARVSTQKRTID
                     VQVNGAIYTEILKNLELAKIALRKETPFIQIIDRPYYPLERSRIGRLLGAILGTFIFG
                     FGIVFFLVIKKSFKEE"
     gene            7339..8538
                     /locus_tag="PLU36_02645"
     CDS             7339..8538
                     /locus_tag="PLU36_02645"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HMZ45681.1"
                     /translation="MFIGHSFFAFSQWIQLSVVTKFCTSLDLGIYTLGLAIIGPLFIF
                     AGLQLKAIQITDVTTNWKFQEYFTLRVICLLAAIIFVIVYSLLFSREFFSLFLLLALL
                     KVVEGISEIMNGQQQLQEDMKKVAISNILRGFAIAVGVFIGVYFIKSLVVGLLIALLG
                     NLLVLVFYDYKNCTTLLKTKQLIQFATTRYKSIVLKALPLAIVVTLIYLNANVAKYFI
                     EYLKSTVEQGIYSTIGYLIVIGNLVNNALGQAITPRLSKYFAEREMKKFNQLAKQFII
                     ANIVIGILIIAVVHFTGEYILLFFFNNSIAQYTQLFTLFMVAGLLLYCVSCIGYILTS
                     MHYIKQQPIIHIVGLATNLICCFLLIRIYGLNGAAYAWIISFAAQLIISVLLLNKSYK
                     NILKPAV"
     gene            8584..9612
                     /locus_tag="PLU36_02650"
     CDS             8584..9612
                     /locus_tag="PLU36_02650"
                     /inference="COORDINATES: protein motif:HMM:NF026247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HMZ45682.1"
                     /translation="MAVITAFSYINKLNKTDVFAYLMYSFMLLMAVLRIDTGADYATY
                     IEMHERVGKSGFQDMLDFTEPLFFWLLKLIYVFTDDYWIMFAVVSFFIYTVLFLALKK
                     LSTDIALSLILYVITSYYFISINQLRQSISLVIFLYSLTYIINAKPGKYFLLNLIGVG
                     FHYSAAIVLFLYPLFRINFSRRNLIIIYLASIVLSIFIEKMFVSSLSALDLFYAQYFD
                     MEEYMEKNYLALGKVVIPNLILIYSIIQFKNFSKAEYSIFLVFFASIIYYNIFFGKHI
                     FIRPGMYFEIVTILYIPIFIKKFFSITMGLVVETFLIIYYFFAVTLYGILINGGQGVV
                     PYKFIFSR"
     gene            9609..10691
                     /locus_tag="PLU36_02655"
     CDS             9609..10691
                     /locus_tag="PLU36_02655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ45683.1"
                     /translation="MRIVHIAYFDFEYKTGIGEVVVNLAKAQQQNGNDVFILNTLSQN
                     NIENYENIGVNIVTNTFNFKNYIQTIKPNVVVFYSFYQLPFLKFYKILKALKIPYFIQ
                     PQGAFGKANQQKGYLKKKLANYLFFNAFIQNSNGILYLNNAEKSISVFNWFKASYILP
                     NGVFTNVNYLQNKTPNLPINLIFLSRIDLMHKGLDILLDALLKVDKQLAEKNISLQIY
                     GYGEEADVNYVKKRIPDFTCNINLNNPVFGNEKENVLCNADIFVLTSRYEGMPVAILE
                     ALAYGVPCFITPGTNMAEIIKENNAGWVTSLNTDEIGVNLLNAIEEYKINFSTLRNNA
                     RNCAVNNYQWSVVAEKSIQIYQQAVS"
     gene            10725..11576
                     /locus_tag="PLU36_02660"
     CDS             10725..11576
                     /locus_tag="PLU36_02660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ45684.1"
                     /translation="MTNTTHNQYEPFSVLMATYYKDNPQFLRAALNSLFTQTVLPSEI
                     VLVIDGSIPNSIQEVINDYLKQQPTIFKTFPLDKNVGLGNALNYGLKMCNNELVARMD
                     SDDICVHNRFEIQLNYFKNNPTVSAHGGWMNEFEIELNDRNKIKSTPIGLDKIIKYAR
                     LRNPLNHPTVMFKKSAVLSVNSYIEINLFEDFYLWLRMLNKGLLLANSKDILVNFRVN
                     DSMIARRHGLNYLKKELQFIIKCYRENLIPFNSLLVQILTRLPLRLMPMGALKFIYKY
                     ILRNNSN"
     gene            11585..12286
                     /locus_tag="PLU36_02665"
     CDS             11585..12286
                     /locus_tag="PLU36_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HMZ45685.1"
                     /translation="MQAIILAGGLGTRLKSVVANKPKVLSPVAGKPFLHYIITYLQNQ
                     GVEKYIFALGYLSEQVIDFLKDEYPSLNYEYTIEDTPLGTGGGIRKAIQLITEENVLI
                     VNADTFFEVDIKALHSFHLSTNAHCTISLKQMYNFERYGCVELDNNSNTILSFKEKTF
                     TTKGYINAGYLVINKNYFLSATNNLPQIFSFEKDFLEKNLAKMLIKGLAFDGYFIDIG
                     IPEDFVLAQEKFKNI"
     gene            12388..13359
                     /locus_tag="PLU36_02670"
     CDS             12388..13359
                     /locus_tag="PLU36_02670"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMZ45686.1"
                     /translation="MNVCVIGGSGFIGSHLINSIATHHNVTNVDKQLPEYNNVNVANV
                     IADIRDYDALKLAIPDNSDYIILLAAEHRDDVSPISLYYDVNVEGTHNVLRVMAEKNI
                     EHIIFTSSVSVYGLNKKDPDELSGTDPFNHYGKSKLEAEKVLRRWFDNDRNGKSLIIV
                     RPTVTFGPGNKGNVYNLLKQIASGNFIMVGKGRNKKSMAYVENVAGFISYCLDKKFKG
                     YHLFNYIDKPDLSTKELVHIAEEALGKKLPPIRIPYFIGYTGGLTLDLISRITKKKFP
                     ISAVRVKKFCATTQFSSATIQATNYTPKKQLSEGLSITIKSIVNGSK"
     gene            13406..14515
                     /gene="gmd"
                     /locus_tag="PLU36_02675"
     CDS             13406..14515
                     /gene="gmd"
                     /locus_tag="PLU36_02675"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HMZ45687.1"
                     /translation="MKKALITGITGQDGAYLAEFLLKKGYEVHGIKRRSSLFNTQRID
                     HFYEDPHIPNRHLTLHFGDLTDSTNLIRIIQEVQPDEIYNLAAMSHVHVSFETPEYTA
                     NADGIGTLRILEAVRLLGLINKTKIYQASTSELYGLVQAVPQSETTPFYPRSPYAAAK
                     LYAYWITVNYREAYNMYACNGILFNHESPLRGETFVTRKITRAVAAIALGFQEQLFLG
                     NLNAQRDWGHAKDYVEAMWLILQQEKPEDFVIATGKTTYIRDFVKMAFAEIGIELEFN
                     GEAENEEGKVIANTGKYNIPIGKIVVKVDKRYYRPTEVDLLIGDPTKAQTKLGWKPKY
                     TLAEMVKEMVANDIIVYSKERMLKDNGFSTKSLSQ"
     gene            14522..>14832
                     /locus_tag="PLU36_02680"
     CDS             14522..>14832
                     /locus_tag="PLU36_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMZ45688.1"
                     /translation="MKKDSKIYIAGHRGMVGSAIVRNLQQKGFSNIIYKTSNELDLRN
                     QQAVNDFFEKEKPQFVFLAAAKVGGIVANNTYRADFIYENLMIEANIIHAAFINKVEK
                     L"
BASE COUNT         5457 a         1957 c         2433 g         4985 t
ORIGIN      
        1 aaaacaatac ttgaatgcct aagaaaaaaa tattgctttt atacgaatat ttttatcctg
       61 ggtttaaagc tggtggtcca atacaatctt tggtaaattt gcttttggct atgcaacatc
      121 agtatgaatt ttatgttgct actactgctt atgatatgca aactgcaaca ccttatccaa
      181 acgtagtatg caataaatgg gttaatacac aacttacgcc aaatgcttta cctgtaaagg
      241 tttggtattc atctaatata aaaccagggt acaaagagtt attgcaaact atactaacat
      301 ctggctgtac tactatttat atcaatggtt tttataccaa ttatttttta tttccattat
      361 tgtatttaaa gttgggaaaa ttaaagaata tggattgcat tgttgctcca agaggtatgc
      421 ttacaaaagg ggcattgagt gtaaaaccat ttcgaaagaa aatttatttt actgtaatta
      481 aatgttttgg tcttttaaaa actgttcgtt atcatgctac caatacagaa gaagtacaag
      541 aaatacaatc tatgtttgga agaagttctt caattacttt agcttcaaat gtttctaaac
      601 ctccttttga aaaattcagg caatctacca aaaataatga tgagttaaga ttagtttttt
      661 tagctctaat atcaccgatg aaaaatttat tactgctatt acaatattta cagtcttgca
      721 gtaatactat tgtgttggat atttatggtc ctgtaaaaga taaagcctat tggcaacaat
      781 gtttacaagc tatacaagct ttaccaaaaa atattaaagt acattataaa ggcgatgcag
      841 agccacatac agtgcaaaat attttcacag gctacgatgc aatgattcta ttaaccaaag
      901 gcgaaaattt tggtcatgct atttatgaaa gtttaagtgt aggtacacct gtaattataa
      961 gccattttac accatggaat aatttgcaag aaaaaaaagc aggttggaat attaatataa
     1021 ataataaaga agaattacta accactttac atcaattatc aaaaatgaca ttacaggaac
     1081 ataatgattg tagcaacaat gcttggcacg ttgcaaaaga ttattatgaa caatcaaatt
     1141 atacaaaaaa ttacaacaat ttatttagtt aaaaactatg attattttag gtattaatgc
     1201 atatcatgca gactcatctg cagcaatttt taaagatgga attatgattg cagcaacaga
     1261 agaagaacgt tttagaagaa taaaacactg ggctggtttt ccatctatgg caatagagtt
     1321 ttgtttaaag gaagtaggag ttactctgaa agaagtagat catattgcaa taggaagaga
     1381 ccctaaagca aaatttttaa agaaactttt tttctttgca cagcaccctg ttcactccat
     1441 aaaatttgtt gcagaaagat ggagtaatca aaaacaagta acaagtattg aaaaagaatt
     1501 agcacaactt tctggattag atgaaactga aataaaaaat aaaattcatc aggtagagca
     1561 tcatagaagc catttagcaa gtgccttttt tgcctcacct tttgaagaag ctgctttgct
     1621 tagtattgat ggctcaggcg atttttcaac aaccatgtta ggcattggaa aaggcaataa
     1681 aattgaagta attgattctg ttgatttccc tcattctatg ggaatttttt atacagcttt
     1741 tacacaactt ttaggcttcc cttattatgg agatgagtat aaagtaatgg gtttagcacc
     1801 ttatggcgaa cctaagtatg ttgataaatt aagagatgtt gttttatgga aagacaatgg
     1861 attatttcaa ttaaactttg cttatttccg ttcagcaaca cagggcttta taaaatatga
     1921 aaacaatttg cctattgtac caccattatt tggtaaaaaa atggttgaag tttttggagc
     1981 agaaagaaaa aaagatgaac cattatcaca ataccataaa gatgtagcag cctctgtaca
     2041 acgattcact gaggaaacca tatttcatat tttaaaagtg ttacataaaa gaacaggact
     2101 tgataaagtt tgtattgctg gaggtgttgc acaaaatagt gttgctaatg gtaagttaac
     2161 aagaaacaca ccttttaaag aagtttatat tccctcggca gggcatgatg ctggtatttc
     2221 aatgggtgct gctcaatatg tttatcatca aatatttaat aaaccaagag tagctccaat
     2281 ttacagtgct tatactggag caaaattttc taacgatgaa attgaagttt tattaaaaac
     2341 aaaaaatatc aactacaaaa aatacgatga tgaaactttg tacaatgttg ttgcagatag
     2401 attaattaat gcaggagtgg taggttggtt taatggtaga gcagaatttg gtccaagagc
     2461 tttaggagca agaagtattt tggctgaccc cagaagaaac gatgctaaag atttattaaa
     2521 tgcaaaaatt aaacgtagag aaagttttag accttttgca ccaagcattt taaaagaatt
     2581 tgtagaagaa tattttgaag taaatgatat tgttcctttt atggaaaaag tttttccaat
     2641 taaaaaagag aaacaacatt taattcctgc tgtaacacat gttgatggaa caggaagatt
     2701 acaaagtgta gatagtaata catcgccagc ttatcatagt ttaataaaaa atttttataa
     2761 caaaacagga gttcctatct tgctaaatac ttcttttaat gaaaatgaac caatcgttaa
     2821 ttcccccctc gaagcattgg attgttttct tagaacatca atggatatgt tagtgttaga
     2881 gaatattgta atagaaagat aaattaataa atgaaagtct ccatcattac cgctacctat
     2941 aacagtgcaa caacagttgc tgatacttta ctgtctatag aaaagcaaac ttatttgcca
     3001 aatattgagc atataattgt agatggactt tcggcagata atactttaga aattgtaaat
     3061 cattttcaac atgtaactaa agtaatttca gaaaaagata aaggtattta cgatgcaatg
     3121 aataaaggta ttgcattagc caatggtgat attattggca ttttaaattc tgatgatttt
     3181 tatgcacatg ataatgttat ccatcgtgta gtggaaatat ttaacactac aaattgtgat
     3241 gcagtttatg gcgatttaat ctttgttcat cccaataatc caaataaagt tttacgtaaa
     3301 tggattgcag gaagttttaa tgccaatatg tttttaaaag gttggatgcc tcctcatcct
     3361 actttttttg taagaaaaga ggtatatgaa aagtatggta aatttaatct tacattaaaa
     3421 agttctgcag attatgaact attgttaagg tttttgtaca agcatacaat aaaagtagag
     3481 tatttaagag atgtaattgt aaaaatgcgt agtggtggtc aaagcacaaa atccatagca
     3541 aacagaatta gagcacacaa agaagattat atggcttgga aaataaataa tttaaaacca
     3601 aaatggttta cacttttatt taaaccagca cgaaaaatta aacaattcct ttatccaaga
     3661 atttaattac taatttcgtt tttgagttta ttttcaaatt aatattgcat aatgaaagtt
     3721 tttcttttta ccattagagt tttcttacta atagtttgta tttcttttgt atcaaatgtg
     3781 gcagcacaaa gtggctcatt acaagaactt atcaacagca gagatttatc aaactttaaa
     3841 gtagaaatgc tgaaagatga agacattatt aagtacaaaa cctatttgca aaatgcagga
     3901 attactgaat cgcaagcaga gcaaatggct ttacaaagag gtatgccatc ttcagaaatt
     3961 ttaaaattaa aagcaagagt tgcaagttta tctagcagaa ctactactac cacaaagcca
     4021 actaccaaag gaagaactaa tacgagtaga gatatagact ctttagatgt agagggtagt
     4081 agagtagttg aaaaagaaaa agttttaaaa cccgaagatg aaatttttgg ttccgcttta
     4141 tttaataata atgatttgcg ttttgaacct gatttaagaa ttgccactcc taaaaattat
     4201 attataggcc cagatgatga aattgcaatt gatgtgtatg gatatcaaga aaccaatcca
     4261 agacttacag tatctcctga aggatttata aatataccca atgtaggttt tgtttcagta
     4321 aatggtttaa ctgtagaaac tgctgcaaaa aggattaaag aaaaaatgat taaaaatggg
     4381 tattcaagtg cagcaacggg caacactaaa attgaagtaa gcattagtaa aattagaact
     4441 attagagtta ctgtaattgg agaagctaaa aaacctggta cctataatgt gtcttctcta
     4501 tcatccttat ttaatgtgtt gtatgcagca ggtggcccaa gtaataaagg tagttttaga
     4561 accatagaat taataagaaa taataaagta atagccacct tagatgctta cgattttcta
     4621 ttaaaaggca ttcaacaaaa taatgttaaa ctaaccgatc aggatgttgt tagaatacca
     4681 gcagccaata ttttagtaaa gcttaaagga gaagtaaagc accaaggaat ttttgaaatg
     4741 ttgccaaccg aaaatatgaa tagcttatta agctttgcac aaggctttac aggtaaagct
     4801 tatacagcat cggttaatgt taagtcgttt acggatattg aaagaaaaat tcaagacatt
     4861 aataaaacag aatttataaa ttataaaccc aaaaatggag atgaagtttt tgtaggaaga
     4921 gttttagaaa ggtatagtaa cagagtaatt ttagaaggtg cagtatatcg tcctggacaa
     4981 tatgaactaa cttccaacct tacattaagt aaactaatag caaaagcaga tggcttaaaa
     5041 gaagatgctt ataaagaaag aggattgtta tatagaacaa acgaagactt aacaaaacaa
     5101 gtaatagact ttaatttgaa tgatgttaat agcaataata atcaagatat tattttgcaa
     5161 aaagatgatt caattgtaat tgcttcggcc aaagattttt atgaaaccta tactttaaca
     5221 gtagatggag aagtaagaaa accaggtgtg tacgaatact ataaaggcat tactttaaaa
     5281 gggttaatat ttcaaacagg aggctttacc gatgctgcta cagcacaaag aatagaaatt
     5341 gcaagaagaa ttaaaagcga tgatattaat gccactgcaa tagcccaggt tatagaaatt
     5401 gcaaccgaaa aagatttaag tattaaaggc gaagaagtaa aattgcaacc ttgggatgta
     5461 gttattgtaa gaagtaatcc aggctataaa cctcaaattg ctgtaaaagt agaaggagaa
     5521 gttttatatc caggaaccta tattttaggg tctaaagaag ataaagtaag cgatatcata
     5581 aaacgtgcag gtggcgtaac accacactcc gataaaacag gagcatttat aactcgtatt
     5641 aataccagtg catttaaaga tgaagctgcc gaaaggttac aaaaactaaa aaaggcaagc
     5701 gatacttcta ctcaacttat tgaagaatta actaaaccaa cagtaaaaat tagtttaaga
     5761 ttagatgaag tattaaataa taaaggcaac ttattagaaa atattacttt aatggaaggc
     5821 gatgtaatac atgtacctaa acaaagaaat gtagtgaagg taaatggaga agtaatgttt
     5881 cctacagaaa ttgtgtataa agaaggagca agcatacact attatattga taaagcagga
     5941 ggctttacag aaaatgccag aaagaaaaaa gtaacggtgt taaactccaa tggtagtgca
     6001 gctaaaacaa agaaattttt atttttcaga agttacccaa gtgtattgcc aggttcagaa
     6061 atattagttc caaaaattcc ggtaaaagaa aataaaggat taagcactgc agaatggttg
     6121 gctgttgcaa gtggtttagc atctttggca ggtgttgcag ttgcaattat aaatgtaagt
     6181 agaaaataat ttttttttaa taaattttat tatttgtgag tttagtaaac cctgaagaaa
     6241 aagttgtaaa agcaaaacac ctaattgcac tcatgaaata ttggtggaag tttttatatg
     6301 ctaaaaaagt attgataata gctattacat ttttgggtgc agtagtaggt tttacttttg
     6361 gatggtttca aaaaccaaat tataaagcag aattaacttt tgcaccagaa gatgctggcc
     6421 aacttggagg ttatttagga attgctgcac aatttggcat tgatttaggc atgggcagtg
     6481 gtaatgtttt tgaaggagaa aatattaccg aattatttaa aagcagcact ttaatttcaa
     6541 atacacttta ttcagaagct accataggcg gtaaaaatca aaccttaatt gcttataaaa
     6601 ttgcttctaa atacaaaaaa aataagcctg ttttagattc gttacaatta caccaagtta
     6661 atttcaatca atctctacaa aacaaagctt taggaattag gtttgccgat agtttagtga
     6721 aagcaattat taaagaaaca tccaaatcct tagatgttaa taaagttgat aaaaaactaa
     6781 acatcattaa agttagtttt acggataaag atgaagtttt tgcaaaatta tttgtggatt
     6841 tattagcaca aaatgcaata aaatattata cagggtataa aagtaaaaag gcaagagata
     6901 atgttgaaat cttacagcat caggccgact ctgttaaaag aatgttgttt ggaggaatag
     6961 gagatgttgc aagtatgaat gatttgaatg taaatcctac taaacaaact gcaagggtaa
     7021 gtactcaaaa aagaactatt gacgtacaag tgaatggtgc aatttataca gaaattctta
     7081 aaaatttaga gttagctaaa atagctttaa gaaaagaaac accttttatt caaattattg
     7141 atagacctta ttacccatta gaaagaagca gaattggtag gttattaggt gccattttag
     7201 gtacattcat ttttggtttt ggtattgtat tcttcttagt tattaaaaaa tcttttaagg
     7261 aagaataaca gagtaaattt tattttctaa aatttcattt ttatttctac aaaaagcatt
     7321 aagcatagca tatcatggat gtttataggg cattcatttt ttgcattttc acagtggata
     7381 caattatcag tagttactaa attttgtacc tcgttagatt taggtattta tacattgggc
     7441 ttagctatta taggccctct ttttattttt gcagggcttc aactaaaagc aattcaaata
     7501 acggatgtaa caacaaattg gaaatttcaa gagtacttta cattacgtgt tatttgccta
     7561 ttggctgcaa ttatttttgt tattgtctat agcttacttt tttcaagaga atttttttca
     7621 ctttttttat tacttgcatt attaaaagtt gtagaaggta tttctgaaat tatgaatggc
     7681 cagcaacagc tacaagaaga tatgaaaaaa gtagctattt caaatatatt aagaggtttt
     7741 gctattgcag tgggggtgtt tattggcgtt tacttcatta aaagtttagt agtagggctt
     7801 ttaattgcat tactcggtaa ccttcttgta cttgtttttt acgattataa aaactgcact
     7861 actttactta aaactaaaca acttattcaa tttgcaacaa cacgttataa aagtattgta
     7921 ttaaaggcat tgccattagc tattgtagtt accctaattt atttaaatgc taatgtggct
     7981 aaatatttta ttgaatattt aaaaagtact gttgaacaag gtatttattc tacaattggt
     8041 tacttaattg taattggtaa tttagttaac aatgctttag ggcaggcaat tacaccaagg
     8101 ttaagtaaat attttgcaga aagagaaatg aagaagttta accaacttgc aaagcagttt
     8161 ataattgcaa atattgtaat tggtatctta attattgcag tggttcattt tactggagaa
     8221 tatattttat tatttttttt taataattca attgcacaat acacccaact atttacattg
     8281 tttatggttg caggtttact gctttattgt gtttcttgta ttggatatat tttaaccagt
     8341 atgcattata ttaagcaaca accaataatt catattgttg gcttagccac aaatttaatt
     8401 tgttgttttt tactaattcg tatttatggt ttaaatggtg cagcttatgc ctggattatt
     8461 tcctttgctg cacaactaat tattagtgtt cttttattaa ataaatcgta taaaaatatt
     8521 ttaaagcctg ctgtttaagt aatgcacttt gctttttctc aatattttat ttatatagtt
     8581 tttgtggctg taattacagc cttctcttat attaataaat taaataaaac agatgttttt
     8641 gcatacttaa tgtattcctt tatgctgtta atggctgtat taagaattga tacaggagca
     8701 gattatgcaa cgtatattga aatgcatgaa agagttggca aaagtggctt tcaagatatg
     8761 ttggatttta cagaaccact ttttttctgg ttacttaaat taatttatgt atttaccgat
     8821 gattattgga taatgtttgc tgttgtatct ttttttatat acacagtttt atttttagca
     8881 ttaaaaaaac tttctacaga tattgcctta tcgttaatac tatatgttat tacttcttac
     8941 tattttattt ctatcaatca gttaaggcaa tccatatctt tagtaatttt tttgtacagt
     9001 ttaacttata taataaatgc caagccagga aaatatttct tgctaaattt aattggtgta
     9061 ggctttcact attcagcagc cattgtatta tttctatatc ccttatttag aattaatttt
     9121 tctcgtagaa atttaattat catttacttg gcatctattg tactttctat ttttatcgaa
     9181 aaaatgtttg tatcctctct atcggcttta gatttatttt atgcccagta ttttgatatg
     9241 gaagaataca tggaaaaaaa ttatttagct ttaggcaaag tagttattcc aaatttaatt
     9301 ttaatttatt ccattataca gtttaaaaac tttagcaaag ccgagtactc cattttttta
     9361 gtattctttg caagtattat ctattacaat atattttttg gcaaacacat ttttatcagg
     9421 ccaggaatgt attttgaaat tgtaacaata ttgtatattc caatatttat aaaaaaattc
     9481 tttagtataa caatgggttt ggttgtagaa acatttttaa ttatttatta cttctttgct
     9541 gtaacactct atggtatttt aattaatggt gggcaaggag tagtgcctta taaatttatt
     9601 ttttccagat gagaattgta catattgctt attttgactt tgaatataaa acaggaattg
     9661 gagaagtagt tgtaaatctt gctaaagcac aacaacaaaa tggaaatgat gtatttattt
     9721 taaataccct atctcaaaat aatatagaaa attacgaaaa tattggagtt aatattgtta
     9781 ctaatacttt taattttaaa aattatattc aaacaattaa gccaaatgtt gtagtttttt
     9841 attcttttta tcaactgcct tttttaaagt tttataaaat tttaaaagca ttaaaaattc
     9901 catattttat acagcctcaa ggtgcctttg gcaaggctaa ccaacaaaag ggttatttaa
     9961 aaaagaaact tgctaattac ttatttttta atgcatttat acaaaactca aatggtattt
    10021 tatacttgaa taatgcagaa aaaagtattt ctgtttttaa ttggtttaaa gcttcttata
    10081 ttttaccaaa tggtgttttt actaatgtaa attatttgca aaataaaact ccaaatttgc
    10141 caattaacct tattttttta tctagaatag atttaatgca taagggttta gacatattgt
    10201 tagatgcttt attaaaagta gataaacaac ttgctgaaaa aaatatcagt ttgcaaattt
    10261 atggttatgg agaggaagct gatgtaaatt atgtaaaaaa aagaattcca gattttactt
    10321 gcaatatcaa tcttaataac cctgtttttg gtaatgaaaa agaaaatgtt ttatgcaatg
    10381 cagacatttt tgttttaaca tcaagatatg aaggcatgcc tgttgcaatt ttagaagcat
    10441 tagcgtatgg agtgccttgt tttattacac caggtaccaa tatggcagaa ataattaaag
    10501 aaaacaatgc tggttgggta acttcattaa atacagatga aataggagtc aatcttttaa
    10561 atgcaattga ggagtataaa ataaattttt caaccctaag aaataatgct agaaactgtg
    10621 ctgttaataa ttaccagtgg agtgttgtag cagaaaaatc aattcaaatt taccaacaag
    10681 cagttagtta gaaagcaaat taacccttaa cctccctttc ttaaatgact aatacaaccc
    10741 ataatcaata cgagccattt tctgtgttaa tggccactta ttataaagat aatccacaat
    10801 ttttaagagc cgcattaaat agtttgttta cacaaacagt acttccatca gaaattgttt
    10861 tagtaataga tggtagcatt ccaaactcta ttcaagaggt tattaatgat tatcttaaac
    10921 aacaaccaac aatttttaaa acctttcctt tagataaaaa tgttggtttg ggcaatgcat
    10981 taaattatgg cttaaaaatg tgtaacaatg aattagtggc tagaatggat agtgatgata
    11041 tttgtgtaca taaccgattt gaaattcagt tgaattattt taaaaacaat ccaactgtat
    11101 ctgcacatgg tggctggatg aatgaatttg aaattgaatt aaacgatagg aataaaatta
    11161 aatcaacccc aattggttta gataaaatta taaaatatgc aaggctaaga aacccactca
    11221 atcacccaac agtaatgttt aaaaaatctg ctgtacttag tgtaaattct tatattgaaa
    11281 ttaatttgtt tgaagatttt tatttatggt taaggatgtt aaataaaggc cttctattag
    11341 ctaactcaaa agatatttta gtaaacttta gagtaaacga tagcatgatt gccagaagac
    11401 atgggcttaa ctacttaaaa aaagaattac agtttattat taaatgctac agagaaaatt
    11461 taattccttt taattcatta cttgtacaaa tattaacacg tttgccttta aggctaatgc
    11521 ctatgggggc tttaaagttt atttataaat acattttaag aaataattca aactaaagtt
    11581 tacaatgcaa gccattattc tcgcaggagg tttaggtact cgtttaaaat cggtagtggc
    11641 aaataagcca aaagtattat ctccagttgc tggtaaacct ttcttgcatt atatcattac
    11701 ttatttacaa aaccaaggcg ttgaaaaata tatttttgct ttaggatatt tgagtgagca
    11761 ggtaattgat tttttaaaag atgaataccc ctcattaaat tatgaatata caatagaaga
    11821 tacaccatta ggcacaggtg gaggaattag aaaagcaatt caattaataa ctgaagaaaa
    11881 tgttttaatt gtaaatgccg atactttttt tgaggtggat attaaggctc tgcactcttt
    11941 tcatctttct accaatgcac attgtactat atctttaaaa caaatgtata attttgaaag
    12001 gtatggttgt gttgagttag ataataactc taacacaatt ctttctttta aagaaaaaac
    12061 ttttacaact aaaggatata ttaacgcagg gtatttagta ataaataaaa attatttttt
    12121 gtcggcaacc aataatttac cccaaatatt ttcttttgaa aaagattttt tagaaaaaaa
    12181 tttggctaaa atgctcatca aagggttagc ttttgatggc tattttatag atattggaat
    12241 accagaagat tttgtactgg ctcaggagaa atttaaaaac atatagttaa gctaagaaat
    12301 ttaataacaa atcaacccaa taagaaattt tgttgatttt tgtatagtgc aattccttat
    12361 cttgcttttt taattttagg ataataaatg aatgtttgtg taattggagg ctcaggattt
    12421 attggctcgc atctaattaa ttctattgca acccaccata atgtaactaa cgttgataag
    12481 caactaccag aatacaataa tgttaatgtt gcaaatgtta ttgcagatat aagagattat
    12541 gatgcattaa aacttgctat acccgataat tctgattaca taattttatt agcagccgaa
    12601 catagagatg atgtttcacc aatttctctt tattatgatg taaatgtaga aggtacgcat
    12661 aatgtgttaa gagtaatggc tgaaaaaaat attgaacata ttatttttac cagttctgtt
    12721 tctgtatatg gtttaaataa aaaagacccc gatgaacttt ctggcaccga cccttttaac
    12781 cattatggta aaagtaaatt agaagctgaa aaagttttaa ggcgttggtt tgataatgat
    12841 agaaatggta aatccttaat cattgttagg cctacagtaa cctttggtcc aggtaataaa
    12901 ggaaatgttt acaacctttt aaaacaaatt gctagtggta attttattat ggttggcaaa
    12961 ggccgaaaca aaaaatcaat ggcttatgta gaaaatgttg ctggtttcat cagttattgt
    13021 ttagataaaa aatttaaagg ctatcattta tttaattata tagataaacc agatttatct
    13081 actaaagaat tagtgcatat tgccgaagaa gctttaggca aaaaattacc gcctattaga
    13141 attccttatt ttattggcta cacaggtggc ttaacattag atttgatttc tagaattacc
    13201 aagaaaaaat ttcctattag tgcagttaga gttaaaaagt tttgtgctac tacacaattt
    13261 tcttctgcca ctattcaggc taccaactac acccctaaaa aacaattatc cgaagggtta
    13321 tcaattacta ttaaaagtat tgtaaacggt tcaaaataat acttaattag ctattattaa
    13381 aggttagtat aaatttaatt tagaaatgaa gaaagcatta attactggca ttacagggca
    13441 agatggtgca taccttgccg agtttttatt aaagaaaggt tacgaagtac atggcattaa
    13501 acgcagaagt tcgttattta atacacaacg tatagatcat ttttacgaag atccacatat
    13561 tcctaatcgt catttaacat tgcattttgg cgatttaaca gacagcacca atttaataag
    13621 aattatacaa gaagtacagc cagatgaaat ttataatttg gcagcaatga gccatgtgca
    13681 tgtaagtttt gaaacaccag aatatacagc taatgcagat ggtataggca ctttaagaat
    13741 tttagaggct gtaagattat taggtttaat aaataaaaca aaaatatacc aagcctcaac
    13801 ttctgaattg tatggtttag tgcaagcagt acctcaaagc gaaacaactc cattctatcc
    13861 tcgtagccct tatgctgcag caaaattgta tgcttactgg attacagtaa actacagaga
    13921 agcttataat atgtatgctt gcaatggaat tttattcaac catgaatctc ctttacgtgg
    13981 cgaaactttt gtaacaagaa aaattactag agcagtagct gcaatagctt taggttttca
    14041 agaacaatta tttttaggta acttaaatgc tcaaagagat tgggggcatg caaaagatta
    14101 tgtagaagct atgtggctta ttttacaaca agagaagcca gaagattttg taattgcaac
    14161 aggaaaaaca acttacataa gagattttgt aaaaatggct tttgccgaaa taggcattga
    14221 attagaattt aatggcgaag ctgaaaatga agaaggtaaa gtaattgcta atactggtaa
    14281 atacaatatt cctattggaa aaattgtagt taaagtagat aaaagatatt ataggcctac
    14341 agaagtagat ttattaatag gagatcctac aaaagctcaa acaaaattgg gttggaaacc
    14401 taaatatact ttagcagaaa tggtaaaaga aatggtagcc aacgatatta ttgtttactc
    14461 aaaggaaaga atgttaaaag ataatggctt ctccactaaa agtttatcgc aataaagtaa
    14521 tatgaagaaa gattctaaaa tttatattgc tgggcatcgt ggaatggtag gcagtgctat
    14581 tgtgcgaaac ttgcaacaaa aaggttttag taatattatt tacaaaacgt ctaatgaatt
    14641 agatttaaga aaccaacaag cggtaaatga tttttttgaa aaagaaaaac cacaattcgt
    14701 ttttttagct gctgcaaaag ttggtggaat tgttgccaac aatacctata gagccgattt
    14761 tatttatgaa aatttaatga tagaagccaa tattattcat gcagctttta taaataaagt
    14821 agaaaaatta tt
//
LOCUS       DAOQPK010000031        14775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_2070_length_14775_cov_4.141644, whole genome shotgun sequence.
ACCESSION   DAOQPK010000031 DAOQPK010000000
VERSION     DAOQPK010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 14775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14775
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2070_length_14775_cov_4.141644"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1843
                     /locus_tag="PLU36_02685"
     CDS             <1..1843
                     /locus_tag="PLU36_02685"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HMZ45689.1"
                     /translation="FLGASVEFGSYARGFESFQASVYAGAGDVSKSVSALVEEIERAK
                     RFGLTQAELDRAKTNLLTRMERMNNEKDKTESADFVEEYIRNFLTNEPIPGIATEYEL
                     HKTLLPTITLADVNAIGNSLKNNSNFVTAVTGPIATDGVTLPKDEELVTIVTSAASKQ
                     DIKPYEEKEIAKNLLSKEPKAGKIISSTKNIKLGTTTYVLSNGIKVTVKKTDFKNDQI
                     LLSARRFGGLNNYGIADKYNANFLTSVISSMGIGAFSPTDLRKALTGKTATINPVFGE
                     YNEGFSGSSSVKDFESMLQLLNLYVTEPRKDTALFKSFIQKSKSQTKFMMANPQVTFI
                     DTFYKAMYKNNPLAPIAIPKGEYYDKINLDRSLAIYKERFGDASGLHFVLVGSLPKDT
                     TEMLIEKYIASLPVSNKKFNFKDNGLRTAKGKININVHKGKEEKALILAVYSGEVPYS
                     ESLSLKAEAISEVLNIKIIEELREKIQGIYGGGIYSSVEKYPYNNYNYVAQLPCGPEK
                     ADTLVIALNNEINNIKTKGPSVEDLNKVKQQWIEQYKTSIKENRTWLTKIQAITLEGA
                     SVDYFLNYEKIVNALTAAQIKATANLLFNGKNVITAVLKPEEKKGEE"
     gene            complement(1922..3232)
                     /gene="aroA"
                     /locus_tag="PLU36_02690"
     CDS             complement(1922..3232)
                     /gene="aroA"
                     /locus_tag="PLU36_02690"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HMZ45690.1"
                     /translation="MQAIIHPSQINGIITAPASKSIMQRACAAALIKKGKSIIYNYGN
                     SYDEKTALEIIQCLGATTIVNNNKICIESNGLYAVNNNIHCNESGLSVRLFTFLAATL
                     NEEITINANGSLLKRPMHFFKEIFPLLNVTIHQSNNIFPFFVKGPLLPTTIEINASIS
                     SQYLTGLLFAYSAALQNKYQTDSFLNEQICIKANNLNSKPYINLTLQVMKHFGLFTPE
                     VVGENRFCFNSKNYERSNANTTTEFTVEGDWSNAAFLLVAAAIAGKVKVNGMNFISEQ
                     ADRRIVEVVEKIGCNFWVNNESIQVEKKHLNCFDFDATHCPDLFPPLVALACNCKGIS
                     TIKGVNRLLYKESNRAEALQTEFAKMGVEILVDGDIMIIKGDNEIKGSNLNSHQDHRI
                     VMACTIAALNASQNTKICNVEAVNKSYPLFFDHMKKLGTRIELY"
     gene            complement(3238..4248)
                     /gene="aroB"
                     /locus_tag="PLU36_02695"
     CDS             complement(3238..4248)
                     /gene="aroB"
                     /locus_tag="PLU36_02695"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HMZ45691.1"
                     /translation="MIKQTYLFKYSATNIFFNMPISSLTKLVDVKQCVIITDENIFAL
                     YKVKFKKYNTIVLKAGEEYKVQQTADSIIKQLLEFDADRNTILIGVGGGVITDITGYV
                     ASIYKRGVQLGFVPTTLLAMIDASIGGKNGIDVGHYKNMVGCFKQPNFILFDFLFLKT
                     LPQKQWQQAFAEIIKHACIKDAALFKEIQINNLQFYRSNKKALIALIEKNIVIKLGIV
                     QKDELENGERKLLNFGHTLAHALENLYQLNHGNAVSIGIAFAVKLSQKITGFNQTSAV
                     IELLNKYNLPVELNYKASKVFEVLKKDKKRNQQELHFVLLNKIGKAVIVPIPLQEIEK
                     LL"
     gene            complement(4305..5360)
                     /locus_tag="PLU36_02700"
     CDS             complement(4305..5360)
                     /locus_tag="PLU36_02700"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HMZ45692.1"
                     /translation="MFTKRPFIIAGPCSAETQEQLMQTAINLKSNTNIDLFRAGIWKP
                     RTRPGQFEGVGKVGLQWLQQVKNETGLATTIEVANTVQVDEALKHNIDVLWLGARTVA
                     NPFSVQEIATALQGVNIPILIKNPTNPDLELWTGAVERISKAGITNIGLVHRGFSVYG
                     NDEYRNAPMWHLAIEMKRRHPEKMFLCDPSHIAGNRNILQAISQKSIDLNYDGLMIES
                     HISPDNAWSDAKQQITPNILGSLLAKLIWRNEFAECNEDKNLLENLRQSINLLDDELI
                     QLLGKRMKIADTIGRFKKDNNITILQTSRWNEILLKIFSKGEELGLSKEFLTKYFDAV
                     HMESINHQNKVMNDDIQ"
     gene            complement(5401..7068)
                     /locus_tag="PLU36_02705"
     CDS             complement(5401..7068)
                     /locus_tag="PLU36_02705"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMZ45693.1"
                     /translation="MITFSALGLDEKLVKATDALGFEHATPIQEKSIPVLLSGTKDMI
                     GLAQTGTGKTAAFGLPLLQLVQVTEKYPQALIVCPTRELCLQIVNEIEQFKKFISGMH
                     VVAVYGGASIGMQIRELKRGVQIVVATPGRLIDLIERKAINLEQIKYVVLDEADEMLN
                     MGFQEDIEFILQNTPQRESTWLFSATMPGEIKKVTKKYMKNPFEVTVGKVNTANKSID
                     HQYFITQAQHRYETLKRLIDFNPGMYGIIFTRTKADAQEIAEKLTREGYDIDALHGDL
                     SQPQRDKVMNAFRDKTLQLLIATDVAARGIDVKEITHVINYELPDDTEVYTHRSGRTG
                     RAGSTGVCMSIVHAREIYKLRNIERFIQTKFNKLEIPSGKDVCRKQFYTFMDKLLQTD
                     ISHGEYATFLPMLQEKFAEVSKEEVLQRVAALEFDRFLKYYENAEDLNIREQKRDTKR
                     EFGGDGRDQARSRGRDNKGVKYSGGNGFARLFVNLGTKDGFYKASFLQFILDMSDLRK
                     DVLGRIDMKEMNSWIEIDKKAAKQMIQSIDGKNYRGRKIRMNDANSR"
     gene            complement(7162..7659)
                     /locus_tag="PLU36_02710"
     CDS             complement(7162..7659)
                     /locus_tag="PLU36_02710"
                     /inference="COORDINATES: protein motif:HMM:NF040422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6438 domain-containing protein"
                     /protein_id="HMZ45694.1"
                     /translation="MKKFFYLLVAALIFQACCVCNKMSAVDAMQFDKIVFHSSTCFGS
                     CPQIAIEINREGGTKLKRQFFNESATQTKERSGEFKGHISKEALDGLMLELNHSNYET
                     LQFPNVDCCDAPVITIIVYAKGKKVYLRSMMPPKDAGDLIKYLTELTTTVELPLAEDK
                     FEIEN"
     gene            7769..9616
                     /locus_tag="PLU36_02715"
     CDS             7769..9616
                     /locus_tag="PLU36_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HMZ45695.1"
                     /translation="MIRLLLVVGLLICNNIIAQTTNNSISIIPLPNNITIGKGYFTLN
                     NLTTINYTTAESKKLALLLQEYLQVQYNLNIKITEKATNNCIQFITNKKTNNEAYDLV
                     ISTNKIEIIGYDAGLFYGYQSLLQLLPVTIKNKEILLPIVTIKDTPRFEYRGMHLDVC
                     RHMFPLSFLKKYIDILASYKLNVFHWHLTDDQGWRLEIKKYPKLTSIGAYRNQTLIGN
                     YRTVGEYDNTAYGGFYTQEQAKELVAYARERFVTIIPEIEMPGHALAALAAYPTLGCG
                     DKTTNFQTAQTWGVFDDVFCAGKEETFELLQNVIDEVIEIFPSKYIHIGGDECPKTKW
                     KTCSSCQKRIKDNNLKDEHELQSYFIQRMEKYINNKGRKIIGWDEILEGGLAANATVM
                     SWRGDAGGIAAANQNHDVIMTPASYGLYFDHKQGADRNREPLSIGGLSTLEKVYLSNP
                     IPAVLEPTKHKFIKGVQANVWTEYIATTNKVEFTIYPRIFALAEIAWTSQEHKNWTNF
                     SQTRVANHLAKLDKTNTFYRVPEVYGLKDTIIYASEYIFKNLTPSVEGAKIYYTIDDY
                     NPSALDNEFINEVKITVPPSKERIFKAIVVTPAGKTSNYVKAVIINSKN"
     gene            9687..10811
                     /locus_tag="PLU36_02720"
     CDS             9687..10811
                     /locus_tag="PLU36_02720"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HMZ45696.1"
                     /translation="MITKLPRLAIKIIFIIITCLVLVLFLPALLSFWVSPQEFWVIGF
                     LTLIFPYTATILALLCIFWFISKPKFGWFILIVFLLGYKHYCAIFSFTSIKDFMLNKQ
                     TKDIRIISWNIQSFNGISKTKEAKKNSKNDILNCINKYKPNIVCLQEFNTSNLSADAD
                     NLSTFKKYYPYYYFSEDYKRKKGSYISGCIIFSTYPIIKTGKTKYPFAESLIYADIKI
                     NSDTIRVYTSHLQSFKFKKEDYDELDNISQHNEASKTASKNLFNKMKLAFKHRAEQAN
                     IVNSVTSADNKPSIICGDFNDVPASYTYNTIKGNRQDAFLNNIFGIGKTFTSLAPTLR
                     IDYILPDNYFKINQFDLVDENLSDHLMLVTDVMLHNNKSH"
     gene            10889..11374
                     /locus_tag="PLU36_02725"
     CDS             10889..11374
                     /locus_tag="PLU36_02725"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HMZ45697.1"
                     /translation="MAIKIFITGGTFDKEYNELNGTLFFKDTHMPEILKLGRNKANVD
                     VRTLMMIDSLEMTQEDRMMIIYQCQQCIEDKIIITHGTDTMAETAELLAQTVFNKTIV
                     LTGAMIPYKFGSSDGLFNIGNAIAFVQTLPYGVYIAMNGLYFNAGRVRKNRQTGSFES
                     I"
     gene            11525..11680
                     /gene="rpmH"
                     /locus_tag="PLU36_02730"
     CDS             11525..11680
                     /gene="rpmH"
                     /locus_tag="PLU36_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HMZ45698.1"
                     /translation="MKQTFQPHKRRRKTVHGFRKRMQTANGRKVLASRRAKGRKKLTV
                     SDERGLK"
     gene            11750..12142
                     /gene="rnpA"
                     /locus_tag="PLU36_02735"
     CDS             11750..12142
                     /gene="rnpA"
                     /locus_tag="PLU36_02735"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HMZ45699.1"
                     /translation="MLKKYSYNKKEKLKSKKLLDEVFKKGKTFSIYPVKVFYLVTNDE
                     LESNNHLKTGVGVSKRYFKQAKKRNYIKRVLRECYRQNKLPLVDHLKNNKKHLALFFL
                     YTDKEVLSFIEIEKRVKNILDKLQQQLN"
     gene            12139..12369
                     /gene="yidD"
                     /locus_tag="PLU36_02740"
     CDS             12139..12369
                     /gene="yidD"
                     /locus_tag="PLU36_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HMZ45700.1"
                     /translation="MKYLLIILSYPFLLLIKLYQYIISPMLGPKCRFTPTCSQYAAEA
                     LKKYGIFKGSWLALKRISKCHPAGSSGYDPVP"
     gene            12381..13472
                     /locus_tag="PLU36_02745"
     CDS             12381..13472
                     /locus_tag="PLU36_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HMZ45701.1"
                     /translation="MQEFLDKIILNNNVRSYATVFAIIIIAFIFKRYLTKYIAGWLYK
                     LVSNKEIKVKKESFLNKVIKPLDAFLILLVSIIAVDKLNFPEILDFKILKISFKTIIE
                     SVSNAAMVIVFIWLCRRMIDFIAIILEEKANATEDPTDNQLIVFFKDFFKVILVIIGI
                     LLILRFSFHKNIGNLLTGLSIVGAAIALSLRESLENLIASFIIFFNKPFVTGDVVKVH
                     NFTGTVEKVGLRSTRIRTDVKTFVTVPNKQMVDSILDNITLRTQRKIDIRLEISLSTS
                     ISQLKELTPLLQNIVTPNSVIENSLVYFLETGKNAHVFVVEVYAAMFQNINEFNVLRE
                     EINLKIIELLENQQIEFAATIADIKLHNK"
     gene            13714..>14775
                     /locus_tag="PLU36_02750"
     CDS             13714..>14775
                     /locus_tag="PLU36_02750"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HMZ45702.1"
                     /translation="MRKVLLAFLLLNIFFSIKAQNKISYYRSKGKLPMMSYSKGSDRL
                     GGAKMNIIDTNILLKIIDSTHEFFTVQLSKNHTSYLEKEFAIPDTLVKPKNYYLCNSI
                     NIKSIEDSFDLVSINMEEKVPYKIWMENNPNKIMLQLYGVQSNINWINQRINTKSVKN
                     VTINQTEDDVITVTIELSNKQHWGFSTSYIHNTLNIKVKHAPKPEIKNLIIAIDAGHG
                     GNNKGTIGSKTKIEEKDYTLIYAKELEKLLTKKGAKVIMTRTTDTSFGNKDRILFLQQ
                     YSPHLLISLHFNASVKPEINGVSTYYKHIGFKPLAQSILQKMLELDLNEFGNIGQFNF
                     SLNAITDFPNCLVEIAFLSN"
BASE COUNT         5269 a         2279 c         2067 g         5160 t
ORIGIN      
        1 atttttaggt gcatctgttg aatttggctc ttatgcaaga ggttttgaat cttttcaagc
       61 ctctgtgtat gctggtgcag gtgatgtttc aaaatctgtt tctgctttag tagaagaaat
      121 agaaagagca aaacgttttg gtttaacaca agcagaatta gatagagcaa aaaccaattt
      181 gctaaccaga atggaaagaa tgaataacga aaaagacaaa acagaatctg ctgattttgt
      241 agaggaatat attcgtaatt ttttaactaa cgaacctata cctggtattg ctacagaata
      301 tgaattgcat aaaacattat taccaacaat tactttggcc gatgtaaatg caattggaaa
      361 tagtttaaaa aataatagca attttgttac agccgttaca ggcccaattg ctacagatgg
      421 tgtaactcta ccaaaagatg aagaacttgt aactatagta actagtgcag catctaaaca
      481 agatattaaa ccttacgaag aaaaagaaat tgccaaaaat ttattaagta aagaacctaa
      541 agccggcaaa attatttctt ctacaaaaaa tataaaactt ggtactacta cgtacgtttt
      601 aagtaatggc attaaagtta ctgttaagaa aacagatttt aaaaacgacc aaattttatt
      661 aagtgcaaga cgttttggtg gtttaaataa ttatggtatt gcagacaaat acaatgcaaa
      721 tttcttaacc tcagtaatta gttctatggg tattggagca ttttcgccaa cagatttgcg
      781 taaagcatta actggtaaaa ctgcaacaat taatcctgtt tttggtgaat ataatgaagg
      841 gttctctggt agtagttctg taaaagattt tgaatctatg ttacagttat taaatttata
      901 tgtaacagag ccacgtaagg atacagcttt atttaaaagt tttattcaaa aaagtaaatc
      961 gcaaactaag tttatgatgg ctaaccctca ggttactttt atagatactt tttataaagc
     1021 aatgtataaa aataatccgt tagctcctat agctattcct aaaggtgagt actacgataa
     1081 aataaacctt gaccgttcat tagccattta taaagaacgt tttggcgatg caagtggttt
     1141 acattttgtt ttagtaggta gcttaccaaa agatactacg gaaatgctaa ttgaaaaata
     1201 tattgctagc cttcctgttt ccaataaaaa attcaatttt aaagataatg gcttacgtac
     1261 agcaaaagga aaaattaata ttaatgtaca taaaggaaaa gaggaaaaag ctttaatact
     1321 tgctgtgtat agtggagaag tgccttatag cgaatcttta tccttaaaag cagaagctat
     1381 tagcgaagtt ttaaatatta aaattattga agagctgaga gagaaaattc aaggtattta
     1441 tggtggtgga atttacagta gtgttgaaaa atatccttac aataattaca actatgttgc
     1501 tcaattacct tgtggccctg aaaaagcaga tacacttgta attgctttaa acaatgagat
     1561 taataatatt aagactaaag gtccatctgt agaagacttg aacaaagtaa aacaacaatg
     1621 gatagagcaa tacaaaactt ctataaaaga gaacagaact tggttaacta aaattcaagc
     1681 tattacttta gaaggtgcta gtgttgatta cttccttaac tatgaaaaaa tagtaaatgc
     1741 attaacagca gcacaaatta aagcaactgc aaatctttta tttaatggta aaaatgtaat
     1801 aactgcagtt ttaaaaccag aagaaaagaa aggtgaagaa taattaactt ctttcaccaa
     1861 caaaataaag ccgaatgaat caaaaacatt cggctttatt tttttaaaaa tatactatag
     1921 cttaataaag ttctattcta gttcctaatt ttttcatgtg gtcaaaaaac aatgggtaag
     1981 atttgtttac ggcttctaca ttacaaattt ttgtgttttg actagcattt agtgctgcaa
     2041 ttgtgcaagc cattacaatt ctatgatctt gatgagaatt aagattggat cctttaattt
     2101 cattgtcacc tttaataatc attatatctc catccaccaa tatttcaact cccattttag
     2161 caaattcagt ttgcaaagct tctgccctat tactttcttt atatagtaac ctattaactc
     2221 cttttatggt agaaattcct ttacagttgc aagctagagc cactaagggt ggaaataaat
     2281 ctggacaatg agttgcatca aaatcaaagc agtttaaatg ttttttttca acttgaatag
     2341 attcattatt tacccaaaaa ttacacccaa ttttttctac aacttctact attcttctat
     2401 cggcttgttc cgatataaaa ttcataccat tcacttttac cttgccagca atagcagcag
     2461 ctaccaataa aaaagcagca ttactccaat ctccttcaac tgtaaattca gtggttgtgt
     2521 ttgcattact tctttcataa ttcttactat taaagcaaaa cctgttctct cctaccactt
     2581 ctggagtaaa taagccaaaa tgcttcataa cttgtaaggt aaggtttatg taaggcttac
     2641 tattaaggtt atttgctttt atacaaattt gctcattcaa aaaactatct gtttgatatt
     2701 tattttgtaa agcagcacta taagcaaata ataatccagt taaatattga gaacttatac
     2761 ttgcatttat ttcaatagta gtgggtagta atggtccttt aacaaaaaat ggaaatatgt
     2821 tattgctttg atgaatggta acgtttaaga gaggaaaaat ttctttaaaa aaatgcatag
     2881 gtctttttaa taaactacca tttgcattaa tagtaatttc ctcatttaaa gttgctgcta
     2941 aaaatgtaaa tagccttaca cttaacccac tttcgttaca atgtatatta ttatttactg
     3001 cataaagccc attgctttca atacaaattt tattgttatt aactattgta gttgctccta
     3061 agcattgaat gatttctaat gctgtttttt catcatagct gttaccataa ttataaatta
     3121 tgcttttacc ttttttaatt agtgcagcag cacaagccct ttgcataata cttttagatg
     3181 caggggcagt aataatgcca ttaatttgtg atgggtgaat gatagcttgc acaagtatta
     3241 taataatttt tcaatttctt gtaaaggaat aggtacaata acagctttgc caattttatt
     3301 taataaaaca aaatgcaact cttgttgatt tcttttttta tccttcttca gtacttcaaa
     3361 aacttttgat gccttgtaat ttaattccac aggcaagtta tacttattca ataattcaat
     3421 aactgcactt gtttgattaa atcctgtaat tttttgggaa agtttaaccg caaatgcaat
     3481 acctatagaa actgcatttc catgatttaa ttgataaagg ttctccaatg catgtgctaa
     3541 tgtatgacca aaatttagca atttcctttc tccattttct agttcatctt tttgaacaat
     3601 gcctaatttt ataacaatat ttttttctat taaagctatt aatgcttttt tattactcct
     3661 ataaaattgc aaattattta tttgaatctc tttaaataat gcagcatctt ttatacaagc
     3721 atgtttaata atttctgcaa atgcttgctg ccattgtttt tggggtaaag ttttcaaaaa
     3781 caaaaaatca aataaaataa aattgggttg tttaaaacag ccaaccatat ttttatagtg
     3841 gccaacatca attccatttt ttcctccaat actagcatct atcattgcaa gtaaagtagt
     3901 aggtacaaac cctaattgta caccacgttt ataaatgctg gcaacataac ctgtaatatc
     3961 agtaattaca ccaccaccaa caccaattaa aatagtgttt ctatctgcat caaattctaa
     4021 taattgttta ataatactat cggctgtttg ttgtactttg tactcctctc ctgcttttaa
     4081 aacaatagtg ttatattttt tgaattttac tttatataat gcaaaaatat tttcatcggt
     4141 aatgattaca cattgtttaa catccaccaa tttagttaaa ctgctaattg gcatattaaa
     4201 gaagatgttg gtagcagaat atttaaatag ataggtttgt ttaatcatac agcttacttg
     4261 ataaaaaata atggtttaaa gttaatgttt aaaaccgtgc catgctactg tatatcatca
     4321 ttcattactt tattttgatg attgatactt tccatatgaa ctgcatcaaa atatttcgtt
     4381 aagaactctt tacttaaccc caactcctct ccttttgaaa atatttttaa gagtatttca
     4441 ttccatctgc tggtttgtaa aattgtaata ttattatctt ttttaaatct ccctattgta
     4501 tcggcaatct tcattctttt ccctagtaat tgtataagtt catcatctaa taggttaatg
     4561 ctctgtctta aattttctaa caagttcttg tcttcattac attctgcaaa ttcatttctc
     4621 caaattaatt tagcaagtaa acttcctaat atatttggtg taatttgctg tttagcatca
     4681 ctccaagcat tatcaggaga aatatggctt tctatcatta agccatcata attaaggtca
     4741 atactttttt gtgaaatggc ttgtaaaata tttctattgc cagcaatgtg agaaggatcg
     4801 catagaaaca ttttttctgg atgccttctc ttcatttcta ttgccaaatg ccacatagga
     4861 gcatttctgt actcatcatt tccatacaca ctaaaacctc tatgcactaa accaatattt
     4921 gttatgccag ccttactaat tctttccact gcaccagtcc aaagttctaa atcggggttt
     4981 gttggatttt taattaaaat aggaatattt acaccttgta aagcagtagc aatttcttgt
     5041 acactaaaag ggttagcaac agttctggca ccaagccaaa gtacatcaat attatgtttt
     5101 aaagcctcat caacctgtac agtatttgcc acctctattg tagtagctaa gcctgtttca
     5161 tttttaactt gttgtagcca ttgtaaacca accttgccaa ctccttcaaa ctgccctggc
     5221 cttgttcttg gcttccaaat tcctgctcta aataaatcaa tattagtatt gctttttaaa
     5281 ttaatggcag tttgcattag ctgctcctgt gtttctgcag aacatggccc agcgataata
     5341 aaaggtcgtt ttgtaaacat agtttaaagc ttgtttacct ttaaatttta agcaaaaata
     5401 ttaacgagaa ttagcatcat tcattctaat ttttcttcct ctatagtttt taccatctat
     5461 agattgtatc atttgtttag ccgctttttt atcaatttca atccagctat tcatttcttt
     5521 catatctatt cttcctaaaa catcttttct taaatcactc atatctaaaa taaactgtaa
     5581 aaagctagct ttataaaatc catcttttgt acccaggtta acaaatagtc tagcaaaacc
     5641 attaccacca ctatatttca ctcctttatt gtctcttcct ctgcttcttg cctggtctct
     5701 tccatcgcct ccaaattctc ttttagtgtc tcttttctgt tctctaatgt ttaaatcttc
     5761 tgcattttca taatatttca aaaacctgtc aaactctaaa gcagctaccc gttgcaatac
     5821 ttcttcttta gatacttcgg caaatttttc ttgtaacatt ggtaaaaatg tagcatactc
     5881 tccatgcgaa atatctgttt gtaatagttt atccatgaaa gtgtaaaact gttttctaca
     5941 aacatcttta ccacttggaa tttctagttt attaaatttt gtttgaataa aacgttcaat
     6001 atttctaagc ttataaattt ctctagcatg tacaatgctc atacaaacgc ctgtgctgcc
     6061 tgcacgtcct gttcttccac ttctatgtgt gtaaacttca gtatcatcag gtaattcgta
     6121 gttaattaca tgggtaattt ctttaacatc tatacctctt gcagctacat ctgttgctat
     6181 aagtaattgt agggttttat ctctaaaagc attcataact ttatctcttt gtggttgaga
     6241 taagtctcca tgtaaggcat caatatcata tccttctcta gttaattttt ctgcaatttc
     6301 ctgagcatct gcttttgttc ttgtaaaaat aattccatac atacccggat taaaatctat
     6361 caaccttttt aaggtttcgt atctatgctg tgcttgtgta ataaaatatt gatgatctat
     6421 actcttattt gctgtattta ctttgccaac tgtaacttca aaaggatttt tcatgtactt
     6481 tttggttact ttttttatct cgccaggcat agtagcacta aatagccaag tactttctct
     6541 ttgaggtgta ttttgcaaaa taaattcaat atcttcttga aagcccatgt ttagcatctc
     6601 atctgcctca tctaaaacta catattttat ttgttctaaa ttaattgctt ttctttcaat
     6661 taaatcaatt aacctacctg gtgttgcaac tacaatttgc accccacgtt ttagttctct
     6721 tatttgcatg ccaatacttg ctccaccata aacagcaaca acatgcatac ccgaaatgaa
     6781 ttttttaaat tgctctattt cgtttacaat ttgtaaacaa agttctctgg tagggcaaac
     6841 tattaaagcc tgaggatatt tttctgttac ttgtactaat tgtaataatg gcaaaccaaa
     6901 agcagccgtt ttacctgttc ctgtttgtgc caaacctatc atatctttag ttccacttaa
     6961 caatacagga atactttttt cttgaatagg tgtagcatgt tcaaagccta aagcatcggt
     7021 tgccttaacc aatttttcat ccaaacccaa cgcactaaat gttatcattt attttaaaaa
     7081 tttgcggcaa aggtaactaa tagcatttac tatgctaatt ttttgtgttc atcagtaaat
     7141 aattgtgcag taaacaggct tttaattttc aatttcaaat ttatcttcag ccaatggcag
     7201 ttcaacagtt gtagttaact ctgttaaata tttaattaag tcgccagcat cttttggtgg
     7261 catcattgaa cgcaaataca cttttttgcc tttagcataa actataattg taattactgg
     7321 tgcatcgcaa caatctacat ttggaaattg aagagtttca tagttactat ggtttaactc
     7381 aagcattaat ccatctaacg cttctttact tatatgtcct ttaaactctc cagaacgttc
     7441 tttagtttga gttgcacttt cattaaaaaa ttgtcttttt aactttgttc ctccttctct
     7501 atttatttct attgcaattt gtgggcaact accaaagcaa gtggaggaat gaaatactat
     7561 tttatcaaat tgcatcgcat caacagcact cattttattg catacacaac atgcttgaaa
     7621 aattaatgca gcaactaata aataaaaaaa ctttttcata ctataaaatt actttatgca
     7681 aattttaaat aaaagtagtt agattaattt cattctttaa atttattctt cgttttaatc
     7741 aaatattttt gacactaaaa tatatactat gataaggcta ttgctggtag ttggtttatt
     7801 aatttgcaat aatattattg cacaaactac taataattct atttccatta ttccattacc
     7861 caataatatt acaataggta aaggatattt tactttaaat aacctaacaa caataaacta
     7921 tacaactgcc gaaagcaaaa aactggcttt attgttacaa gagtacttac aagttcaata
     7981 taacttgaat attaaaataa ctgaaaaagc tacaaataat tgtattcaat ttataacaaa
     8041 taaaaaaact aataatgaag cttatgattt agttatttca accaataaaa ttgaaattat
     8101 tggttatgat gcaggtctgt tttatggata ccaatctcta cttcaacttt taccagtaac
     8161 cataaaaaat aaagaaatat tattacctat tgttaccata aaagatacac caagatttga
     8221 gtatagagga atgcatttag atgtttgcag gcatatgttt ccactaagtt ttttaaaaaa
     8281 atatatagat atattagcaa gttataaact aaatgttttt cattggcatt taaccgacga
     8341 tcaaggttgg cgtttagaaa taaaaaaata tccaaagctt acctctattg gtgcatatag
     8401 aaatcaaact ttaattggaa attaccgaac agttggtgaa tatgacaata ctgcttatgg
     8461 tggattttat acacaagaac aggcaaaaga attggttgct tatgcaagag aacgctttgt
     8521 tactattatt cccgaaattg aaatgcctgg ccatgcatta gctgctttgg ctgcttatcc
     8581 aacattgggt tgtggcgata aaacaactaa ctttcaaaca gcacaaactt ggggcgtttt
     8641 tgatgatgtt ttttgtgccg gcaaagaaga aacatttgaa ctactgcaaa atgttattga
     8701 cgaagtaatt gaaatttttc caagtaaata cattcatatt ggaggcgatg aatgccctaa
     8761 aacaaaatgg aaaacctgta gctcatgtca aaaaagaatt aaagacaata acttaaaaga
     8821 tgaacatgaa ttgcagagtt atttcattca aagaatggag aaatacatta ataataaagg
     8881 tagaaaaatt attggatggg atgaaatact tgaaggtgga ttagcagcca atgctacagt
     8941 aatgagttgg cgtggagatg caggaggaat tgcagcagca aatcaaaatc atgatgtaat
     9001 tatgacacct gcatcgtatg ggttatattt tgatcataaa caaggagcag acagaaatag
     9061 agagccttta agtattggcg gcctttctac tttagaaaaa gtatatctat caaaccctat
     9121 acctgcagtt ttagaaccaa caaaacataa atttattaaa ggcgttcaag ccaatgtttg
     9181 gacagaatat attgcaacta caaacaaggt agagtttacc atttacccaa gaatttttgc
     9241 tttagctgaa attgcatgga caagccaaga acataaaaac tggactaact tctctcaaac
     9301 tagagttgct aaccatttag caaaacttga taaaaccaat acattttatc gtgtaccaga
     9361 agtttatggt ttaaaagata caatcattta tgcatcggaa tatattttta aaaatttaac
     9421 cccatcggtt gaaggggcta aaatttatta taccattgat gattataacc catctgcatt
     9481 agataatgaa tttattaatg aagttaaaat aactgttccg cctagtaaag aaagaatttt
     9541 taaagcaata gttgtaaccc ctgctggtaa aacaagtaat tatgtaaaag ctgtaatcat
     9601 caattcaaaa aattaatact taccttttgt tagtttttta aatagataaa taatcataaa
     9661 caagtaagat ttattatttt taaagcatga taactaaact gcctagactg gctattaaaa
     9721 ttatttttat aattataact tgtttggttt tagtgttgtt tttaccagca ttattaagtt
     9781 tttgggtaag ccctcaagaa ttttgggtta ttggttttct tacacttatt tttccttaca
     9841 ccgctacaat tttagcctta ctttgtattt tttggtttat ttcaaaacca aaatttggct
     9901 ggtttatatt aattgttttt ttattaggct acaagcatta ttgtgcaata ttttccttta
     9961 cgagtattaa agattttatg ttaaataaac agactaaaga tattagaatt atttcgtgga
    10021 atattcaaag ttttaatggt atatctaaaa ccaaagaggc taagaaaaat tcaaaaaacg
    10081 atatattaaa ctgcatcaat aaatacaagc ccaatatagt ttgtttacaa gagtttaata
    10141 catctaattt aagtgcagat gcagataacc tttctacttt taaaaaatat tatccttatt
    10201 attatttttc tgaagattac aaaaggaaaa aaggaagtta tataagtgga tgtattattt
    10261 tttcaaccta tcctataatt aaaactggaa aaacaaaata cccttttgca gaaagcttaa
    10321 tttatgcaga tattaaaatt aattcagaca ccataagagt ctatacttcg catttacaat
    10381 cctttaaatt taagaaagaa gattatgatg agttagataa tattagccag cacaatgaag
    10441 catctaagac tgcttcaaaa aatttattta ataaaatgaa attagctttt aagcatagag
    10501 ccgaacaagc aaatattgtt aatagtgtaa catcagcaga taataaacct tctataatat
    10561 gtggagattt taatgatgta ccagctagtt atacttacaa tactatcaaa ggcaataggc
    10621 aagatgcatt tttaaataac atttttggta taggaaaaac ttttacatct ttagccccca
    10681 ccctaagaat agattatatt ttacccgaca attattttaa aataaatcag tttgatttag
    10741 tggatgaaaa tcttagcgac catttaatgt tagttactga tgtaatgctc cacaacaata
    10801 aaagtcatta aaatcttcta tcctcaaatc aacttttttt tgaaacctaa aactttgtta
    10861 ctttgtttta atcattcaaa taaaaactat ggctataaaa atatttatta cgggtggtac
    10921 ttttgataaa gaatacaatg aactaaatgg caccttattt tttaaagaca cacacatgcc
    10981 agagattttg aaacttggta gaaataaagc caatgtagat gtaagaactt taatgatgat
    11041 tgatagttta gaaatgactc aagaagatag aatgatgatt atttaccaat gccaacaatg
    11101 tatagaagat aaaataatta taacccatgg tacagatact atggcagaaa ctgcagagct
    11161 gttagcacaa acggttttta ataaaactat tgtacttact ggtgccatga ttccttataa
    11221 atttggtagt agtgatggat tatttaatat aggaaatgcc attgcctttg tacaaacatt
    11281 accttatggc gtttatatag caatgaatgg attatatttt aatgctgggc gtgtaagaaa
    11341 aaacaggcaa acaggctcat ttgaaagtat ttagttttga atttcaaggt gttaattatt
    11401 tttttatcaa acttttgcta aattatttat tacccctatc tttgcacacc tttcacaaaa
    11461 tggtggtatg gtgaccacta ttgaaatgaa acaaaattgt taggaaaaga aaactttaaa
    11521 agtaatgaaa caaacattcc aaccacataa acgtcgtaga aaaacagttc atggttttcg
    11581 taaacgtatg caaacagcta atggtcgtaa agtattggca agccgccgtg caaaaggacg
    11641 taaaaaatta actgtttctg acgaacgtgg attaaaataa taaatagaaa caaataaatt
    11701 atatttaagt tctgccttta cagcagaact ttttagtttt atagtatcta tgttaaaaaa
    11761 atattcttat aataaaaaag aaaaactgaa gagtaaaaaa cttttagatg aggtgtttaa
    11821 aaaaggtaaa acattttcta tttacccagt taaagttttt tatttagtaa caaatgatga
    11881 gttggaaagt aataatcatt taaaaacagg tgttggtgta agcaaaagat attttaaaca
    11941 ggcaaaaaaa agaaactaca ttaaaagagt tttaagagaa tgttacaggc aaaataaatt
    12001 gcctttagta gatcatctta aaaataataa aaaacattta gctttatttt ttttatatac
    12061 cgataaagaa gttctatctt ttattgagat agagaaaaga gttaaaaaca tattagataa
    12121 attacagcaa caattaaatt gaaatattta ctaattatat taagttatcc atttttattg
    12181 ctaataaagc tttatcagta tatcatttct ccaatgttag gtcctaaatg taggtttact
    12241 cctacttgct cacaatatgc tgcagaagca ttaaagaaat atggtatttt taaaggtagt
    12301 tggttagctt taaaaagaat tagtaaatgc catcctgcag gaagtagtgg ctacgaccct
    12361 gtaccttaat aaaatatagt atgcaagaat ttttagataa aataatatta aacaacaatg
    12421 taagaagtta tgcaactgtt tttgctataa taataattgc ttttattttt aaaagatatc
    12481 ttacaaaata cattgctggt tggctataca aattggtttc taacaaagaa attaaggtta
    12541 aaaaagaatc cttcttaaat aaagtaatta aaccattaga tgctttttta attttattag
    12601 tttccataat tgctgtagat aaattaaact ttcctgaaat tttagatttt aaaatattaa
    12661 aaatttcttt taaaacaata atagaaagtg ttagcaatgc tgcaatggta attgtgttta
    12721 tatggctttg tagaagaatg atagatttta ttgctattat tttagaagaa aaagccaatg
    12781 caaccgaaga ccctacagat aatcagctaa tagtattttt taaagacttt tttaaagtaa
    12841 tattagttat tataggcatt ttacttatac tacgtttttc atttcataaa aacataggca
    12901 acctacttac aggattaagt attgtgggtg ctgcaatagc actatcgtta agagaaagtt
    12961 tagaaaattt aatagcctct tttatcatat tttttaataa gccttttgta acaggcgatg
    13021 tagtaaaagt gcataatttt actggcactg ttgaaaaagt tgggttaaga agtacaagaa
    13081 taagaaccga tgttaaaact tttgttactg taccaaacaa acaaatggta gatagcattt
    13141 tagacaatat aactttaaga acccagcgta aaatagatat tcgtttagaa attagcttat
    13201 ctaccagcat atctcagcta aaagaattaa ccccattatt gcaaaatata gttacaccca
    13261 acagtgttat agaaaacagt ttagtttact ttttagaaac tggaaaaaat gctcatgttt
    13321 ttgttgtaga agtttatgct gcaatgtttc aaaacattaa tgaatttaat gtattgagag
    13381 aagaaataaa tttaaagata attgaacttt tagaaaacca gcaaattgag tttgctgcaa
    13441 ccattgcaga tattaaacta cataataaat aaacgtttta actaaactac tgtaaaataa
    13501 ttttaaagca atttttaact ctgctgctac attgttgtat ctacacacaa aattttatct
    13561 ataatacaaa cattaaaact tgtttgctaa aaaaatattc tgattttatt tctcttaaaa
    13621 tacttttcgt accttgtaaa catcttggtt taacttgcaa tattttaaat attaccaaat
    13681 aagccaataa taaaaaaaca tttcaacaat ttcatgagaa aagtactatt agcatttcta
    13741 ttattaaaca tttttttttc cataaaagcc caaaataaaa ttagttacta tcgttctaaa
    13801 ggcaaattac ctatgatgtc ttatagcaaa ggctctgata gattaggtgg tgctaaaatg
    13861 aatataattg acacaaatat tttattaaaa attatagata gcacacacga attttttact
    13921 gttcaattat caaaaaatca tacttcctat ttagaaaaag aatttgcaat accagatact
    13981 ttagtgaagc caaaaaatta ctatttatgc aatagtatta atataaaatc tattgaagat
    14041 agttttgatt tggtatccat taatatggaa gaaaaagttc cttataaaat ttggatggag
    14101 aataatccaa ataaaataat gcttcagctc tatggtgtac aaagcaatat caactggata
    14161 aatcaacgta taaatacaaa atctgtaaaa aatgtaacca ttaaccaaac agaagatgat
    14221 gtaataacag ttactattga actatctaat aaacaacatt ggggttttag cactagttac
    14281 atacataata ctttaaacat aaaagttaaa catgcaccca aaccagaaat aaaaaattta
    14341 ataattgcaa tagatgccgg ccatggtgga aacaataaag gaactattgg aagcaaaact
    14401 aaaattgaag aaaaagatta cactttaata tacgccaaag aattagaaaa attacttacc
    14461 aaaaaaggtg caaaagttat tatgacaaga acaacagata ccagttttgg taataaagac
    14521 agaatattgt ttttgcaaca atatagccca catttgctta ttagtttgca cttcaacgca
    14581 tctgtaaagc ctgagattaa tggagtaagc acctattata aacatattgg ttttaaacca
    14641 ttagcacaat ctattttaca aaaaatgctt gaattagacc taaacgagtt tggcaatatt
    14701 ggccagttta atttttcttt aaatgccatc acagactttc caaactgcct ggtagaaata
    14761 gcatttctat ctaac
//
LOCUS       DAOQPK010000032        14652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_2094_length_14652_cov_4.697267, whole genome shotgun sequence.
ACCESSION   DAOQPK010000032 DAOQPK010000000
VERSION     DAOQPK010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 14652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14652
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2094_length_14652_cov_4.697267"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..146
                     /locus_tag="PLU36_02755"
     CDS             6..146
                     /locus_tag="PLU36_02755"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HMZ45703.1"
                     /translation="MLGINTEFKPRFLRQYASLHDITFNAVQQYITDIKSLNFPNQTE
                     SY"
     gene            180..1205
                     /locus_tag="PLU36_02760"
     CDS             180..1205
                     /locus_tag="PLU36_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HMZ45704.1"
                     /translation="MIYRSKAPLRIGLAGGGTDVSPYSDEFGGAILNATISLNAYASI
                     QLEKTGGITLQALDRNEIQQFDFSKTLPINGTVDLLKGVYNRIQKDYGFAHQNFTLST
                     FVDAPAGSGLGTSSTLVVAIIGAFVEMLKLPLGDYDIAHYAYDIERNDLSLAGGKQDQ
                     YAATFGGVNFMEFYNNDKVIVNPLRIRSEYISELENNLVLYFTATSRESATIIKEQQK
                     NVVNKNEKSIEAMHQLKEQAKMMKEALLKGKLNEFGEILDFGFQQKRKMANNISNSNI
                     ENIYDAAKKAGATGGKISGAGGGGFMIFYCPQNTRYKVIETLNSFGGVVKPYAFTKNG
                     LKTWSVH"
     gene            1225..1797
                     /locus_tag="PLU36_02765"
     CDS             1225..1797
                     /locus_tag="PLU36_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HMZ45705.1"
                     /translation="MQQKIKSIIQASITTKQEILNNNSLIQTIDDCVNSIVQAFNNGN
                     KVLFCGNGGSAADAQHLAAEFSGRFYKDRKALPAEALHCNTSYLTAVANDYSYDAIYA
                     RLLDGIGNSGDILIGLTTSGNSKNIIQAFEVANAKNITTIAFTGNTGGDIKGLTKYLL
                     NVPSNNTPRIQESHIMLGHIICELVEEKLF"
     gene            1860..2420
                     /locus_tag="PLU36_02770"
     CDS             1860..2420
                     /locus_tag="PLU36_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HMZ45706.1"
                     /translation="MQLLKNIKNNWTLFLDRDGVINYEKENSYIFNWEEFRFYPEVKE
                     ALKILQQYFATIVVVTNQRGIGKQLMSEGDLQNIHQNMVQEIQNAGGRIDKIYFCSDL
                     ENTSPCRKPNTGMALQAKKDFPAMDFTKSIMVGNKLSDMQFGKNAGMHTVFVATTHPQ
                     TPYPHATIDARFKNLLEFALAIQHAK"
     gene            2543..3505
                     /locus_tag="PLU36_02775"
     CDS             2543..3505
                     /locus_tag="PLU36_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HMZ45707.1"
                     /translation="MLKVGVFGVGHLGKFHLNNWKEIEGIKIVGFFDPNNDAAKEVTA
                     TYGIKRFLDEDKLIDACDIIDIVVPTDWHFEVCSKAVRKGKHVFVEKPLCNTIPEAKA
                     LVEMVREANIKMQVGHVERFNPAYLALKDLSLNPMFIEVHRLAQFNPRGTEVSVILDL
                     MIHDLDIILSLVKSDVKTISANGVAVMTDTPDIANVRIEFNNGCVANLTSSRISMKKM
                     RKMRLFQKDSYIGIDFLEKKTEIIKLKEPTDKNVFAFDVETPKGKKTIAVANPTITEA
                     NAIKLELEAFVDSILNNKQTIVNEIDGYLAMEVAHQILNKINNN"
     gene            complement(3654..5153)
                     /locus_tag="PLU36_02780"
     CDS             complement(3654..5153)
                     /locus_tag="PLU36_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HMZ45708.1"
                     /translation="MKNIKSILAKPFAAIVSKQVKKSIQNAVVDQKSIFTSLIKTAKN
                     TQFGNDHFFKDINNYATFANAVPIRDYEQFKNYIELIKEGKQNILWKGKPIYLAKTSG
                     TTSGVKYIPITKDSISNHINTARNALLCYMNETGNTNFAAGKMIFLSGSPVLERVGNI
                     PTGRLSGIVNHHVPQYLRTNQLPSYTTNCIEEWETKLEKIVEETIQKNMTLISGIPPW
                     VQMYFDKLTEKSGKTIAELFPNFSVMVQGGVNFEPYKAKLFESIGKKIDTIELFPASE
                     GFFAFQNSQQEEGLLLNTNSGIFYEFIPAAEIFTENPTRLCLQDVKLGENYALIINSN
                     AGLWGYNIGDTVKFVSLNPYKIIVSGRIKHFISAFGEHVIAEEVEHALLKAANEENIK
                     IIEFTVAPNVAQGKGKSFHEWFIEFENLPKNLPSFIEKVDKNLRDKNVYYDDLIKGNI
                     LQTLHINCIRKNGFIDYMKSIGKLGGQNKVPRLSNDRKIAEELNGFINN"
     gene            complement(5161..5994)
                     /locus_tag="PLU36_02785"
     CDS             complement(5161..5994)
                     /locus_tag="PLU36_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45709.1"
                     /translation="MKLLLSILTFCSISIQPLFAQYYFNDIVATNNTNQLQQLYVKQN
                     IKTIKTVSKESDGSIINDFFIMQSIHKNGNEITTQSQQSSGSKSVLKSFFENGKIVKT
                     INEEDGVSTTSNYSYIDNKLQSIVSGTTDTFITSNSKEEHIWFYKSNSTPEKMFKIKN
                     GKDTTVVNFISDENNLIIEEQWVRNGKMIENYYYYYNDKNWLTDIVRYNNKAKKMLPD
                     FLYEYDTEGRVSKMIQVIAGGTNYNIWVYTYNSLGLKEKEVCYDKKKQLLGTVEYLYN
                     N"
     gene            6128..6907
                     /gene="mazG"
                     /locus_tag="PLU36_02790"
     CDS             6128..6907
                     /gene="mazG"
                     /locus_tag="PLU36_02790"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HMZ45710.1"
                     /translation="MNHTILSNSFLKLVTIMDELREQCPWDKKQTIHTLRPLTIEELY
                     ELTDAITNNNWENIKEELGDVLLHILFYSRIAKEENKFLLHEVIETICEKLINRHPHI
                     YGNVKVENEEDVKRNWEKIKLNEGKKKSVLSGVPTGLPAIIKASRMQEKAKQVGFEWE
                     NKNDVWKKVEEEMCELQEAIKHNNQQHIEEEFGDVLFSLINYARFLKIDAEGALEKTN
                     QKFMHRFKSMEEIATAKGKSLTNMSLEEMDSIWNEIKKANN"
     gene            6943..10656
                     /locus_tag="PLU36_02795"
     CDS             6943..10656
                     /locus_tag="PLU36_02795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ45711.1"
                     /translation="MLLLVAAILYLVSFIVTLFYNYANNPTRVQEKLSKSIQKKERSF
                     EGFLANKQIIESIIYDTLNNTNKNNLQEYNYGIYIYKQKQQPELIYWNNNNYSIAKSD
                     ILKNDNTFIVRYQNGIFECIKQTINIHNNQFIVAALLPIHRHFFIENKYLKSAFDEYP
                     EFDDYYEITNNSNGYSITNTKGNSIYTISKKAGANNTNYNLITILLRIAAIVCLFFYL
                     HFLCLKTLQKKGFNKAFLLLSTVLILLRTISYFINFPFNFSQLPLFDPSVYASNFLHP
                     SLGDLLINCILIFWLIIFYKVNCTLKNSALLFIIRNKKLQKIVPYIHLVALVIITFLC
                     TSTIKSLVLDSKVSLDVTNFFSLTWFSIVSFIILCFCVLIFYHLSHLLLNAVFNFQIS
                     LFKQLLIIALAGLALLSNQLISVSATSNLLTILWLLIYVVIINFRKVDTSIYLLHSTF
                     FIFWILFFSINVAALLMLQNNYIETEQRKKWAEELAEKIDPNSETLLKIATTNFNDEF
                     LQHNFHRFQEEFSNKFIKDSLVNENFSGYLNKYDTRIYTFDCAFNPLFNDDSLPFSVI
                     RTIVLNQSKSTTIVNLHSFEDAYQKENYLYEKNIVADSGTLGYLFVIVKPKQYKSEAI
                     YPELFKQVKDLSNDFNIHYAYAIYNNGALINSFNNYNFPFAHHVSKTPSGKYKIIKQG
                     SYKELWYSNQSGKTVVIVKKSSTLLELFTLFAYLFCTFLILVALLHIASFLIKTRLKK
                     EAIKQVLQFKISTQIQAIIIMISLFSFIVIGVTTISFFIYRFNQTNQERLSKTIQVMA
                     NQVEEKLKTTTANLAFDDMVDITTLGFGNSLEKVINDVSEIYNTDINLYNNTGTLIAS
                     TQPYIYNKQLLSEKMNPIAFETLNKNKSVRFIQSESIASFKYLSIYLPVNDSDGNTYA
                     FINIPFLNSQAELNQEISGFLSTLMNLNAFIFLLAGAIAYMATNKITASFSLIGNKMK
                     AINFEHKNETIEWKRNDEIGILVNEYNKMVLKLEASAKALAKSEREGAWREMARQVAH
                     EIKNPLTPMKLSIQYLQKSIETKQINTPELIKNVSKTLIEQIEQLARIASDFSQFANI
                     ENTQPEVFNVSEIIASLIKLYNTREDVHFVWHEPSEDFLIYADKSQISRLLKNLISNA
                     IEAGNENKQVEIMITQLINNNNIEIAIKDNGTGIDEALKQKLFTPNFTTKSSGTGLGL
                     AICKGIVERANGTISFTTQKNIGTTFFISLPRT"
     gene            complement(10710..14009)
                     /locus_tag="PLU36_02800"
     CDS             complement(10710..14009)
                     /locus_tag="PLU36_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HMZ45712.1"
                     /translation="MKFYYKTLLLILVVCPILTMAQNNFFTNKNESQFAGSQDLVRPI
                     KPERFRAVSLDVTGLKQFLSTVPMEFSADASTRKSILTLPMPNGGTANFSIVESPMQE
                     PGLAAKFPDIKTYSGQGIDDKTATIKLDWTGFGFHAQILSPISGSIYIDPYSRGNVEN
                     YMSYFKKDLQPSSQFQEVGIEGTTDESFNTTQTTSGYCFSQLRSYRFAVACTGEYAVA
                     VGGSTPALLHSAIVTTVNRVNGVYEQEVAVRLILISNNNLIEFLNPSTDPFNGNDNAG
                     VLINESQSVITSTIGTSNFDIGHTFSTGGGGLAGLGVVCISGSKGRGITGSSNPTGDA
                     YDIDFVAHEVGHQFGGSHTFNSTTGSCGGGNRSASSAVEPGSGVTIMGYAGICGSNDL
                     QPHSIPNFHSKSQEQIGTYAFTGIGSTCGTLIATGNNAPVVNAGSDYTIPANTPFMLT
                     GSATDANGDVMTYSWEQYDLGTAGNWNSGNNPLFRSFEPTLSPTRYFPKIQDILDGTT
                     IGEMLPPSARTMSFRLTARDNRAGAAAICSDETSITVVNSSGFSITSQNSMTTWTANG
                     SNTATITWNVAGTNIAPVNTPNVTILFSADGGLTFPYTIVTSTPNDGSQDIIIPSVKT
                     TNGRFMVKGDGNIFFDINNAAITVNVSCGAVGAVISPSSNVVKEVGSVDLNLALSPAY
                     TTAFNVSGNISTSDAQTTLVQATSGLTCVQYSNQYNYDVYAFTASVSGAYVITRTGSS
                     VFNLYVNSFDPGNLCNNWITSNLQGNSLANGVSVFLTAGMPYYIVVGVYYVSGNPVVA
                     LPHNYSFNMATGPGVLYNGAGIYADPGASYSYSYVIVDNATNNIKAISATANLSNSAT
                     YPVGTYTVYGISYLSAHAAAINGFAGGSFAALTSAITNNPSTTCADLSRNAVTVIVKG
                     TLPVTFTDLTAKKKDNDVILNWGTLTEINSSHFEIERADNGKDFNTKIGTVTSAGNSN
                     AIKNYSLVDKAPLKGMNYYRVKQVDMDGKFSYSNIAVINFERGNAHVTLYPNPAKHTL
                     NVEYTAIRNNKISVQVIDARGSVILQSNYTATIGKNIHSLNVSSLTKGVYMLKTIDTD
                     GNITVSKFIKD"
     gene            complement(14131..>14652)
                     /locus_tag="PLU36_02805"
     CDS             complement(14131..>14652)
                     /locus_tag="PLU36_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HMZ45713.1"
                     /translation="LSGIMIWVILMLLYFSFFKYFFLIVGSPIFAYLSEKTASIIEGK
                     DYPFNFSQILKDIVRGIRISLRNALWQTVYLFSILIIGMIPLVGWFVPVLALLIECYY
                     YGFSMLDYSMERNKKSVFESVNYIGVHKGLAIGNGFVFYTMHLLPVIGWVLAPAYAVI
                     AATLSIYDLKEKS"
BASE COUNT         5324 a         2220 c         2167 g         4941 t
ORIGIN      
        1 atgatatgct tggaattaat acagaattta aaccacgttt tttgagacaa tatgcctctt
       61 tacacgatat tactttcaat gcagttcagc aatatattac tgatattaaa agtttaaatt
      121 ttcctaacca aaccgaaagc tattaacctt aattcatcta tttataataa ttttcaatta
      181 tgatttatcg cagcaaagcc ccattgcgta taggtttggc aggaggaggg acagatgtta
      241 gcccttatag cgatgaattt ggtggtgcca ttttaaatgc aactatttca ttaaatgctt
      301 atgcatctat tcaattagaa aaaactggtg gtataacact tcaggcatta gacagaaatg
      361 aaattcaaca atttgatttt agtaaaacat tacccattaa tggcactgta gatttattaa
      421 aaggcgttta taatcgtatt caaaaagatt atggttttgc tcatcaaaac tttacactca
      481 gcacttttgt tgatgctcca gcaggcagtg gtttaggcac ttcatcaacc ttagtagtgg
      541 ctataattgg ggcttttgta gaaatgctga aattgccttt aggagattat gacattgccc
      601 attatgccta tgatattgaa agaaatgatt taagtttagc aggaggcaaa caagatcaat
      661 atgcagctac ttttggtggt gtgaatttta tggaatttta taataacgat aaagtaattg
      721 taaatccatt aagaatacgt tcagaatata taagcgaatt agaaaataat ttagtactgt
      781 attttactgc tacaagtaga gaaagtgcta ctataataaa agagcaacaa aaaaacgtag
      841 tgaataaaaa tgaaaaaagt attgaagcta tgcaccagct aaaagaacaa gccaaaatga
      901 tgaaagaggc attactgaaa ggcaaattaa atgagtttgg cgaaatatta gattttggct
      961 ttcagcaaaa aaggaaaatg gcaaacaata tatctaacag taatattgaa aatatttatg
     1021 atgcagcaaa aaaagcaggt gcaacaggtg gtaaaataag tggtgccggg ggcggtggtt
     1081 ttatgatttt ttattgccca caaaatacaa ggtataaagt aatagaaact ttaaatagtt
     1141 ttggtggtgt agtaaaacct tatgcattta caaaaaatgg attaaaaaca tggagcgttc
     1201 attaaacgaa aactaaaaat tatcatgcag caaaaaataa aatcaattat acaagcttcc
     1261 attacaacta aacaagaaat tttgaataac aattcactca ttcaaacaat tgatgattgt
     1321 gtgaatagta ttgtacaggc ttttaataac ggaaataaag tgttgttttg tggcaatggt
     1381 ggcagtgcag cagatgcaca acatttggct gctgagttta gtggaagatt ttataaagac
     1441 aggaaagcat tgccagcaga ggctttgcac tgcaacacat cttacttaac tgctgttgca
     1501 aatgattata gttacgatgc aatttatgca agactactag atggaattgg caatagtgga
     1561 gatattttaa taggtttaac aactagtggc aacagtaaaa atattattca ggcttttgaa
     1621 gtagcaaatg caaaaaatat tacaaccata gcatttacag gtaatactgg tggtgatata
     1681 aaagggctta ccaagtattt attaaacgta ccaagcaata atacacctcg tattcaagaa
     1741 agccatatta tgttggggca tattatttgc gaattagtgg aagaaaaatt attttagttt
     1801 tataaccatc agttgaattt tgtagcagca tttgttgcgt ttcacatttg tattttttta
     1861 tgcaattatt aaaaaatata aaaaataact ggactctgtt tttagacaga gatggagtaa
     1921 ttaactatga aaaagaaaat agctatattt ttaattggga ggaatttcgt ttttacccag
     1981 aagtaaaaga agcattgaaa atacttcagc aatattttgc aactattgtt gttgttacca
     2041 atcaaagagg tattggcaaa caattaatga gtgagggaga tttacaaaac attcatcaaa
     2101 acatggtgca agaaatacaa aatgccggtg gcagaataga taaaatatat ttttgttcag
     2161 atttggaaaa tacttcgcca tgccgaaaac ccaatacagg tatggctttg caggcaaaaa
     2221 aagattttcc tgcaatggat tttactaaaa gcattatggt aggcaataaa ttaagtgata
     2281 tgcaatttgg gaaaaatgca ggcatgcata ccgtttttgt tgctactaca cacccacaaa
     2341 caccttaccc ccatgcaact attgatgcaa gatttaaaaa ccttttagaa tttgcattag
     2401 caattcaaca tgcaaaataa tattgataat tttaatacca atatttccac atacccattt
     2461 ttgaacgttt ttacttttag ggtttattta tctttacccc tttaaaaact atcattcata
     2521 gtcttatctt aaataatgta taatgcttaa agtaggtgta tttggtgtag gccatttagg
     2581 aaaatttcat ttaaataact ggaaagaaat agaaggtatt aaaatagttg gtttttttga
     2641 cccaaacaac gatgcagcta aagaagttac tgcaacctat ggcattaaaa gatttttaga
     2701 tgaagataaa ttaatagacg cttgtgatat tattgatatt gtagtaccaa ccgactggca
     2761 ttttgaagtt tgttcaaaag cagtacgtaa aggcaaacat gtttttgttg aaaagccatt
     2821 atgcaacact attccagaag ctaaagcttt agtggaaatg gttagagaag caaatattaa
     2881 aatgcaagta gggcatgtag aaaggtttaa ccctgcttat ttagccttaa aagatttatc
     2941 gttaaaccct atgtttattg aagtacatag gttggcacaa tttaacccaa gaggtacaga
     3001 agttagcgta atactagatt taatgataca cgatttagac attattttaa gtttagtaaa
     3061 aagcgatgtg aaaacaatta gtgcaaatgg tgttgcagtg atgacagata caccagacat
     3121 tgctaacgta agaatagagt ttaacaatgg ctgtgttgct aacttaacca gcagcagaat
     3181 tagcatgaag aaaatgcgaa aaatgcgttt gtttcaaaaa gatagttata taggtattga
     3241 ttttttagaa aagaaaacag aaataattaa acttaaagaa ccaacagata agaatgtttt
     3301 tgcctttgat gtagaaacgc caaaaggtaa aaaaactatt gcagttgcta accctacaat
     3361 aacagaagct aatgcaatta aattagaatt agaagctttt gtagatagca ttttaaacaa
     3421 taaacaaact attgttaatg aaattgatgg ctacttagct atggaagtag ctcatcaaat
     3481 tttaaataaa ataaacaata actaaataac tttttacaat ccttttgttt attcaatctt
     3541 aacaaaaatg aggttcgttt ttaaatagat gtatgtgcaa attaattcta ttacaacatc
     3601 attagtaata gtgataaaac atctgaatga aaatatttat agtaataaca agtttaatta
     3661 tttataaacc catttaattc ctctgctatt tttctatcat tacttagacg gggtacttta
     3721 ttttgccctc ctaattttcc tatacttttc atataatcta taaatccatt ttttctgata
     3781 caatttatat gcaaggtttg taaaatattt ccttttatta aatcgtcgta atacacattt
     3841 ttatctcgta aattcttatc tactttctct ataaagcttg gcaaattttt gggcaaattt
     3901 tcaaactcta taaaccattc atgaaagctt tttcctttac cttgtgctac atttggggca
     3961 actgtaaact caattatttt tatattttct tcattggctg cttttaataa tgcatgttct
     4021 acttcttcag caattacatg ctctccaaac gcactaataa aatgttttat tctaccactt
     4081 acaataattt tataaggatt taaactaaca aattttacag tatcgcctat attataaccc
     4141 cataaacctg cattgctatt aatgattagt gcataatttt ctccaagctt aacatcttgt
     4201 aagcaaagcc tggttgggtt ttcagtaaaa atttctgcag caggaataaa ttcgtaaaaa
     4261 attcctgagt tggtgtttaa caataaacct tcttcttgct gtgagttttg aaatgcaaaa
     4321 aaaccttcgc tggcaggaaa taattctata gtatcaattt tctttccaat gctttcaaat
     4381 aatttagctt tataaggttc aaagtttacc cccccttgca ccattacact aaagtttgga
     4441 aaaagttcgg caattgtttt ccccgatttc tctgttaact tatcaaaata catttgcacc
     4501 catggcggaa ttccactaat taaagtcatg ttcttttgga tagtttcttc tacaattttc
     4561 tccaattttg tttcccattc ttcaatacaa ttggtagtat aagaaggcag ttgattcgtt
     4621 cttaaatatt gtggtacatg atggttcaca attccactta acctacctgt tggaatgttg
     4681 cctactcttt ctaaaacagg gcttccactt aaaaaaatca ttttaccagc agcaaagttt
     4741 gtattacccg tttcgttcat ataacacaac aatgcattac gtgctgtgtt aatatggtta
     4801 gaaatagaat cttttgtaat gggaatatat tttacgccac ttgtggtgcc acttgtttta
     4861 gctaaataaa tgggtttacc tttccataaa atattttgct tgccttcttt aataagttca
     4921 atataattct taaattgttc gtaatctcta attggaacag catttgcaaa agttgcataa
     4981 ttattaatgt ctttaaagaa atggtcattg ccaaattgag tgttttttgc agttttaata
     5041 agactagtaa aaatagattt ttgatctaca acagcattct gaatactttt tttaacttgc
     5101 tttgaaacta ttgctgcaaa aggctttgct aatatagatt ttatgttttt cataagctaa
     5161 ttagttattg tataggtatt caactgtgcc taataactgc ttttttttat cgtaacacac
     5221 ttccttctct ttcaacccta aactgttgta agtataaacc caaatattat aattagtgcc
     5281 gccagcaatt acctgaatca ttttgcttac tcttccttct gtatcgtact catataaaaa
     5341 atcgggtagc atttttttag ctttgttatt ataccttaca atatcagtaa gccaattttt
     5401 atcattataa tagtaataat aattttctat catttttcca tttctcaccc actgttcttc
     5461 aataatcaaa ttattttcat cagaaataaa gttaactaca gttgtatctt ttccattttt
     5521 aatcttaaac attttttcag gagtagagtt agatttataa aaccaaatat gttcttcttt
     5581 actattggat gtaataaaag tatctgtagt accagaaact atggattgaa gcttattatc
     5641 aatataagaa taattggaag tagttgaaac accatcttct tcattaatag tttttactat
     5701 ttttccattt tcaaaaaaag attttaaaac agatttacta cccgatgatt gctgtgattg
     5761 cgtagtaatt tcattgccat ttttatgaat actttgcata ataaaaaaat catttattat
     5821 gctaccatca ctttctttac taactgtttt tattgtttta atattttgtt ttacatataa
     5881 ttgttgtaat tggtttgtat tattggtagc aacaatatca ttaaaataat attgtgcaaa
     5941 caagggttga atgcttatag aacaaaaagt aagaattgaa agtaataatt tcataaaata
     6001 tatttctgcc tcaaaagtag caacaagcta ttcaacattc aaaaatgaat agaagtttag
     6061 taaaactgtg gttaaaaaaa cagtactttt aaagttaaat taacaaaaag aattttaagc
     6121 aaatactatg aatcatacaa ttttaagtaa tagcttttta aagttagtta caataatgga
     6181 tgaactaaga gaacaatgcc cttgggataa aaaacaaacc atacacactt taaggccatt
     6241 aaccattgaa gaattatatg aacttacaga tgcaattacc aataataatt gggagaatat
     6301 taaagaagaa ttaggcgatg tattgttgca tatattattc tacagcagaa ttgcaaaaga
     6361 agaaaataaa tttttattac atgaagttat tgaaacaatt tgcgaaaaat taattaatag
     6421 gcatccgcat atttacggta atgtaaaagt agaaaacgaa gaagatgtaa aaagaaactg
     6481 ggagaaaata aaacttaatg aagggaaaaa gaaaagcgta ttaagtggtg tgcctactgg
     6541 cttgcctgct ataattaaag caagcagaat gcaagaaaaa gccaaacaag ttggatttga
     6601 atgggaaaat aaaaatgatg tttggaaaaa agtggaggaa gaaatgtgcg aattacaaga
     6661 ggcaattaag cataataatc agcagcatat agaagaagaa tttggcgatg tactttttag
     6721 cttaataaat tatgcacggt ttttgaagat agatgcagaa ggtgctttag aaaaaaccaa
     6781 tcaaaaattt atgcataggt ttaaaagcat ggaagaaatt gcaacagcca aaggaaaatc
     6841 attaaccaat atgagtttag aagaaatgga tagtatttgg aatgaaataa aaaaagcaaa
     6901 taattgagca acttaaccaa acttatacat caaaaaaaaa tattgttgtt attagtagca
     6961 gcaatattat atctagtatc ttttatagtt accctttttt ataactatgc caataaccct
     7021 acaagagtac aagagaaact aagtaaatct attcaaaaaa aggaaagaag ctttgaaggt
     7081 tttttagcga ataaacaaat aattgaaagc atcatttacg atactttaaa caacaccaat
     7141 aaaaataatt tacaggagta caactatggc atttatatat acaaacaaaa acaacaacca
     7201 gagctaatat attggaataa caataattac tccattgcaa aatcagacat tctaaaaaac
     7261 gataacacat ttattgtacg ttaccaaaat ggcatttttg aatgtataaa acaaacaatt
     7321 aatattcata ataaccaatt tattgttgct gccttattac ccattcacag gcactttttt
     7381 attgaaaata aatatttaaa aagtgctttt gatgagtatc ctgagtttga tgattattac
     7441 gaaataacca ataatagcaa tggttacagt ataacaaata caaaaggcaa ttcaatttat
     7501 actatttcta aaaaagcagg tgccaacaat actaattata atttaattac tatattatta
     7561 cgaattgctg ccattgtatg tttgtttttt tacttgcact ttttgtgttt aaaaactctt
     7621 caaaaaaaag gatttaataa agcattctta cttttatcca ctgttttaat attgcttaga
     7681 acaatatcct atttcatcaa ttttccattt aattttagtc aattaccact ttttgatcca
     7741 tctgtatatg cttcaaactt tttacatcct tccttaggcg atttacttat taattgtatt
     7801 ttaattttct ggctgattat tttttataaa gttaattgca cactaaaaaa ctcggcttta
     7861 ttattcatta tcagaaataa aaagttacaa aaaattgttc cttacattca tttagtggca
     7921 cttgtaataa ttactttttt gtgtactagt accattaaaa gtttggtatt agattcaaaa
     7981 gtatcattag atgttaccaa cttttttagt ttaacttggt ttagcatagt aagctttatt
     8041 attttatgtt tttgcgtatt aattttttat catttatctc acttattact taatgctgtt
     8101 tttaattttc aaatttcttt gtttaaacaa ttgttgatta ttgctttggc aggtttggca
     8161 ctactaagca accaattaat atcagtttca gcaacgtcta atttgcttac tattttatgg
     8221 ttacttattt atgtagttat tattaatttt cgcaaagtag atactagtat ttatttacta
     8281 cattctacat tctttatatt ctggatatta tttttttcaa taaatgttgc agcattatta
     8341 atgttacaaa acaattatat agaaacagaa caaagaaaaa aatgggccga ggaattagca
     8401 gaaaaaatag accctaatag tgaaacactt ttaaaaattg caactacaaa ttttaatgac
     8461 gaatttttgc aacacaactt tcatagattt caagaagagt tttcgaataa atttattaaa
     8521 gatagtttgg tgaatgagaa cttttctggt tacttaaaca aatacgatac aagaatttat
     8581 acttttgact gtgcttttaa tcctttgttt aatgatgatt ctctaccctt ttctgttatt
     8641 agaacaattg ttctaaatca aagcaaaagc actactattg ttaacttaca tagttttgaa
     8701 gatgcctatc aaaaagaaaa ttatctatat gaaaaaaata ttgtagcaga tagtggaaca
     8761 cttggatatt tatttgttat tgttaaacca aaacaatata aaagcgaagc aatatatcct
     8821 gaattattta aacaggttaa agatttatcc aacgatttta atattcatta tgcttatgca
     8881 atttataata atggagcttt aataaatagc ttcaataatt ataattttcc atttgcacat
     8941 catgtttcaa aaactccttc aggaaaatat aaaataatta agcaaggttc ttataaagaa
     9001 ttgtggtata gtaaccaatc gggaaaaact gttgttattg ttaaaaagag ctcaacactg
     9061 ctagagttgt ttactttatt tgcctattta ttttgcacct ttttaatttt agtggctctg
     9121 ctgcatattg caagtttttt aattaaaaca agattaaaaa aagaagcaat taaacaggta
     9181 ttacaattta aaattagtac acaaattcaa gctataatta taatgattag tttgttttca
     9241 tttattgtta taggcgttac taccatttca ttttttattt atcgttttaa tcaaacaaat
     9301 caagagagac tttctaaaac tattcaggtt atggcaaatc aagtagaaga aaaattgaaa
     9361 actactactg ccaatttagc gtttgatgat atggtggata ttactacact aggttttgga
     9421 aatagtttag aaaaagtgat aaatgatgtt tcagagattt ataataccga tattaattta
     9481 tacaataata caggcacact cattgcctcc acacagccat acatttataa taaacagcta
     9541 ttaagcgaaa aaatgaaccc tattgcattt gaaactttaa ataaaaataa aagtgtaagg
     9601 tttatacaat ccgagtcaat tgcaagtttt aaatatttaa gtatttactt acctgtaaat
     9661 gattctgatg gcaatacgta tgcatttatt aatattccat ttttaaactc tcaggcagaa
     9721 ttaaaccagg aaatttctgg ctttttatct acactaatga acttaaatgc atttatattt
     9781 ttgttagcag gtgcaatagc atatatggct acaaataaaa ttactgcttc ttttagttta
     9841 attggcaaca aaatgaaagc aattaatttt gagcataaaa atgaaactat agaatggaag
     9901 agaaatgatg agattggaat tttagtaaat gaatataata aaatggtttt aaagcttgaa
     9961 gctagtgcaa aagcattggc taaaagtgaa agagagggtg cttggaggga gatggcaaga
    10021 caggttgctc atgaaataaa aaatcctctt acccctatga aactcagtat tcaatatttg
    10081 caaaagtcta tagaaaccaa acaaattaat actccagagc ttattaaaaa tgtaagtaaa
    10141 acattaatag aacaaataga acagttagca agaattgcaa gtgatttttc tcagtttgca
    10201 aatatagaaa acacacaacc cgaagtattt aatgtctcag aaataattgc ttcattaata
    10261 aagttgtata atacaagaga agatgttcat tttgtttggc atgaaccaag tgaagatttt
    10321 ttgatttatg cagacaagtc tcaaataagc cgtttattaa aaaatcttat ttctaatgca
    10381 attgaagcag gaaatgaaaa caagcaagtt gaaataatga ttactcaatt aataaataac
    10441 aacaatattg aaattgcaat aaaagataat ggcactggta tagatgaagc attgaaacaa
    10501 aaattattta ctccaaactt tactactaaa tcatctggta caggtttagg tttggcaatt
    10561 tgtaaaggaa ttgttgaaag agcaaatggt actatatctt ttacaactca aaaaaatatt
    10621 ggtactacct tctttattag tttaccaagg acataaaaaa aatccgatta aaaaaattta
    10681 atcggatttt ttagtagtta cactattatt taatctttta taaatttaga tacagttatg
    10741 tttccatcgg tatctattgt tttaagcata tatactcctt ttgttaaaga acttacattt
    10801 aaactatgaa tatttttacc aatagttgct gtatagttac tttgcaaaat aaccgatcct
    10861 cttgcatcaa ttacttgaac acttatttta ttatttctaa tagctgtata ttcaacattt
    10921 aatgtatgct tagcaggatt agggtataaa gtaacatgag catttcctct ctcaaaatta
    10981 attacagcta tattgctata actaaatttg ccatccatat ctacttgttt aactctataa
    11041 tagttcattc cttttaaagg tgctttatca actaaactgt aatttttaat tgcattgcta
    11101 ttacctgcag aagttacagt tccaatttta gtattaaaat ctttaccatt atcagcacgt
    11161 tcaatttcaa aatggctact attaatttca gtaagggttc cccaatttaa gataacatca
    11221 ttatcttttt tcttagctgt taaatctgta aatgttacag gtaatgtgcc tttaactata
    11281 acggtaactg catttctact taagtcagca caagttgtag atgggttatt agttattgca
    11341 gaggtaagag ctgcaaagct accaccagca aagccattta tagctgcagc atgtgcagat
    11401 aagtaactaa ttccataaac agtataagta cccacaggat atgttgcaga gttacttaaa
    11461 ttagcagttg cactaatagc tttaatatta ttagtggcat tatctacaat tacataactg
    11521 taactatagc tagcaccagg gtctgcataa attccagcac cattatacaa aactccaggg
    11581 ccagttgcca tattaaaact atagttatgt ggtagagcaa caacagggtt accagataca
    11641 taatataccc caacaacaat atagtatggc ataccagcag ttaaaaatac actaacccca
    11701 tttgccaaac tattgccttg taaattagaa gtaatccagt tgttacataa gttaccagga
    11761 tcaaaactat ttacatacaa attaaataca gaagaaccag tacgtgtaat tacatatgca
    11821 ccagatacag atgcagtaaa tgcatataca tcataattat attgattaga atattgtaca
    11881 caagttaatc ctgaggtagc ttgaactaag gttgtttgag catctgatgt agaaatattt
    11941 cctgaaacat taaatgcagt agtataagca ggagataaag ctaaattcaa atctacactg
    12001 cctacttctt ttacaacatt tgaagaaggt gaaattactg caccaactgc accacaagat
    12061 acatttacag ttattgcagc attattgata tcaaagaaaa tatttccatc tcctttaacc
    12121 ataaatcttc cgttagttgt tttaacagaa ggaataataa tgtcctgaga accatcattt
    12181 ggtgtagaag taacaatagt gtatggaaaa gttaaaccac catcagcaga aaataaaatg
    12241 gtaacatttg gtgtattaac aggtgcaata tttgtacctg caacattcca tgtaatagta
    12301 gctgtgttag aaccattggc tgtccatgtt gtcatagagt tttgagaagt gatactgaaa
    12361 ccactactat taacaacagt aatagaagtt tcatcagaac aaattgctgc agcacctgcc
    12421 ctattatctc ttgctgtaag tctaaaactc attgttcttg cacttggtgg taacatttct
    12481 ccaatagtag taccatctaa aatgtcttgt attttaggaa aatatctagt cggagataaa
    12541 gttggttcaa aacttctaaa taaaggatta ttgcctgagt tccaattacc tgcagtgcct
    12601 aagtcgtatt gttcccaact ataagtcatt acatctccat tagcatctgt tgcactacct
    12661 gttaacataa aaggtgtatt tgcaggaatg gtatagtcag aacctgcatt aacaactggt
    12721 gcattattac ctgtagctat taaagtgcca caagtacttc caatacctgt aaatgcataa
    12781 gtaccaattt gctcttggct ttttgaatga aagtttggaa tactatgagg ttgtaaatca
    12841 ttgctaccac aaattcctgc ataacccata atagttacac cactaccagg ctctactgca
    12901 gatgaagcac ttctattacc accaccacag ctgccagttg ttgagttaaa agtatgacta
    12961 cctccaaact gatgacctac ttcgtgagct acaaagtcaa tatcataagc atcacctgta
    13021 gggttggaac tacctgtaat acctcttccc ttactaccac taatacacac tacacctaaa
    13081 ccagctaaac caccaccacc agtactaaaa gtgtggccaa tatcaaaatt agatgttcca
    13141 attgtgcttg taattactga ttggctttcg ttaataagta cacctgcatt atcattacca
    13201 ttaaatggat ctgtacttgg atttaaaaac tcaattaaat tattgttgct aattaatatt
    13261 agcctaacag ctacttcttg ttcataaact ccatttaccc tattaacagt agttacaatg
    13321 gcagagtgca aaagggctgg cgtgctacct cctacagcaa ctgcatattc tcctgtacaa
    13381 gctaccgcaa acctataaga tctaagttga gaaaaacaat atccacttgt tgtttgtgta
    13441 gtgttgaaag attcatctgt agttccttca atacctactt cttgaaattg actagagggt
    13501 tgtaagtctt tcttaaaata actcatataa ttttctacat tccctcttga gtaaggatct
    13561 atatatatgc taccacttat tggagataaa atttgagcat gaaaaccaaa gcctgtccag
    13621 tctagtttaa tagttgcagt tttgtcatca ataccttgcc cactataagt tttaatatca
    13681 ggaaattttg cagctaaacc aggctcttgc attggagact caacaatact aaaattagct
    13741 gtaccaccat taggcattgg taaagttaat atagattttc tggttgaagc atctgcagaa
    13801 aactccatag gtacagtgct taaaaattgc tttaggcctg taacatctaa tgatacagct
    13861 ctaaatcttt ctggttttat gggtctaacc aaatcttgag aaccagcaaa ctgactttca
    13921 tttttgttgg tgaaaaagtt gttttgtgcc attgttaaaa tagggcaaac aactagaata
    13981 agcagtaagg ttttgtagta aaatttcata tataaaattt aagttctgca aattaattaa
    14041 aaggccttat tctaacaaca atattattag aaataccttc ttattaaatt aggaaaatat
    14101 gcttctattg attagaaaca taaagttaca ttagcttttt tcttttaaat cataaatact
    14161 aagtgtagct gcaattactg cataagcagg ggccaaaacc catcctatta ctggtaataa
    14221 atgcatagta taaaaaacaa aaccattacc tatagccaaa cctttatgca ctccaatata
    14281 atttacactt tcaaaaaccg atttcttatt acgttccatg ctataatcta acattgaaaa
    14341 tccataataa taacactcaa ttaaaagagc tagtaccgga acaaaccaac ccactaacgg
    14401 tatcattcct ataattaaaa tactaaacaa ataaactgtt tgccacaaag catttcttaa
    14461 agatattctt atacctctta caatatcttt taatatttgg ctaaaattaa acgggtaatc
    14521 ttttccttct atgatagaag ctgttttttc actcaaatat gcaaaaattg gcgagccaac
    14581 tattaaaaag aaatatttaa agaatgaaaa atatagtaac attaaaataa cccaaatcat
    14641 aatgccactt ag
//
LOCUS       DAOQPK010000033        14334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_2168_length_14334_cov_4.094685, whole genome shotgun sequence.
ACCESSION   DAOQPK010000033 DAOQPK010000000
VERSION     DAOQPK010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 14334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14334
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2168_length_14334_cov_4.094685"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..661)
                     /locus_tag="PLU36_02810"
     CDS             complement(14..661)
                     /locus_tag="PLU36_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45714.1"
                     /translation="MKSTLIILTTFLLSVNTLLAQQRQRVIAECTVEFAVNTDDVKGI
                     DNATLETFKSTSKTLYIKGYQSRVNFNSTSFSQSVIYNKRTGDAVILREIGGNKFLTK
                     LDSLAWKKSNSRFENAQLTLLNETKIILGYECKKAILMLADSVSLNLYYTPGFIPSVS
                     EFEFQFKNVPGFVLEYESQDKSGAKIKYFATKINLNPVSSSKFDIPTSGYRILNN"
     gene            complement(755..2602)
                     /gene="yidC"
                     /locus_tag="PLU36_02815"
     CDS             complement(755..2602)
                     /gene="yidC"
                     /locus_tag="PLU36_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HMZ45715.1"
                     /translation="MKTDKNTIIGFVLLGILFIAFFWYNNKMAQSAIDHENKVKDSLA
                     KVEASKITPEIKAKAIEDSVKRDSTNKILKEGNFTGSANGSEKIETIETDILKINFTN
                     KGGRIQTVSLKKYNSYDSTPVVLGTANDKLGYNINTGNNAVANTNDLYFQLKSNQKNT
                     DGSQTIIYELADSAGRNIQHIFTIAPNTYMIDWKVSLTAANQLLNDGNLNISWQTQTT
                     QHEKSAEYERVQMSNICFLENKEFDYIMNKTEHTFEKPTNWMSVVQQFFNTTLVAENN
                     FNSGNVKWEKHTTDTTKILANVQANFQIKITTAATNTIPLKMYFGPNDYNILKKQAPE
                     MDRIVNLGRDMYSFVRPINQYIIMPVFNFFSSFIGNYGWVILLLTLFIRLITAPLTYS
                     SYLSGAKMKVLRPELDALKKKIGENDKQAFAMEQMKLFREAGVNPLGGCIPALLQIPI
                     FFALFSFFNSNIALRGENFLWAKDLSSYDDVISWGTNIWLVGNHISLFNITAVITSFL
                     ISIYNMGNTPTQDNPALKYMPYIFPFVLFFVFNKMPAALTWYYTVSNLVTLILQFIIQ
                     KYIIDHDKILAKIEAKRSNPKKQSKWQERYTQMVENQKKIQEMKNKTKK"
     gene            complement(2767..4260)
                     /locus_tag="PLU36_02820"
     CDS             complement(2767..4260)
                     /locus_tag="PLU36_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45716.1"
                     /translation="MKDIQKFKPSFLLKLDERLLKNNPTIWSTKIHWVIWYSILFIIL
                     LSIISFVVPLEFRRDSVNGYWIVFVSIISFISFVIWLIYLLQFNVFKRFGNFTALDSL
                     KQLLLYYTCILCFMTFPFVFSVVEVVRVNSTYNSKELINDINKINLTIAQIEYDSIQH
                     NWNRDTVWLVADLNKIDTQQRDSIPKITQHSKYAAIDSATFNSRKKITDSVIDLGNNL
                     YVFVECPVYSFLNIYNAKDIGEDKILKSASIFNLVIKNYKPAPKDSLQSLLKTILNKY
                     SINNYQSSINTEFDEQGNNSHLLSTNEIFKKYNLWSVERSIENIFRIKNRWAFEEMKD
                     ILRVVLYFTLILSLLVYIFRHSTVKTFFLSLLTAKLLTIISGLVIALFDFKGYAVYEW
                     YFFYVILFLFLSIMVFTQSSRKVVTGVAINLFTLIFAFLPLMFVFYYNERARLNYIIN
                     DINYQEKMKTINLYLSISEIVLVVLILLIIPTFIFKMYRKWYALPES"
     gene            complement(4271..5416)
                     /locus_tag="PLU36_02825"
     CDS             complement(4271..5416)
                     /locus_tag="PLU36_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ45717.1"
                     /translation="MKNRVEEIRTFIEYKDFDLARRRLLDACLDTRKESLIQQSINFS
                     KQVGNKAEQNLANFSDKFQDGAEQILQQFSSLSSSVIHTNEQVISANKISKSYTVGGF
                     NLQALSLKAYTNKIIGVVGENGHGKTTLLRCLAGQLQINEGSIDYYFDPKPDYFSIKQ
                     KVAFIPQRIPKWYGKLKDNLHFSASITGNYSKENNLMVDFMLERLSLTSYANLTWSQI
                     SSGYRTRFEIARVLLQKPTLLILDEPLANLDINAQQTMLIDFKYLAKSIYYPLSIILS
                     SQQLHEVEKVADEILFIKNGNCIFQTTCSNNVTHKEVVIEIETNANRDTILQILENNV
                     QVSYNGGYYTITSNQLTANVIMEKLLAQQIAITYFRDITNSTKRLFS"
     gene            complement(5429..5887)
                     /locus_tag="PLU36_02830"
     CDS             complement(5429..5887)
                     /locus_tag="PLU36_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45718.1"
                     /translation="MKGLFFVLLIVSNTAFSQQRVKDSTEVANTFNTILKECKNVNFA
                     DPDIAKYGTFYKVAPYIIYQGKDEKRKWKDYCNYSVEEEKNGVDEICFRINRTINKAD
                     SYKIVQYKTEKESEGLWHILHVSLLLNGKQKQMSFAFLKPEKKFALGDID"
     gene            6025..7416
                     /locus_tag="PLU36_02835"
     CDS             6025..7416
                     /locus_tag="PLU36_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HMZ45719.1"
                     /translation="MKPMLRRKFLQQTSILTIGSIAAPQIIFGAQKLEIKKIRVGLIG
                     VGLRGQNHLDVLLRREDVLVTALADPQPKMLAMANELIAKSGKPKPLIFSNGNYDYKN
                     LLKRDDVDAVIISTPWEWHAEQTIDSLLAGKIPGVEVSGAVKLAECWDIVNAAEKTGL
                     PVMVLENVCYRRDVMAVLNMVRKGMFGELLHAQGGYQHDLRGVKFNDGITPYNSGVEF
                     GEKSFSEAAWRTQHSITRNGELYPTHGLGPVAVMMDINRGNRLTKLSSIATKAKGLHR
                     YIVNHPKGGENHPNAKIKFKLGDIVTTQLQTANGETIVLTHDTNSPRPYNMGFRVQGT
                     NGIWQDNHAGEFTAGLIYFEDKSKPHRWENTEQNMKTYDHPLWKKYEKDTAGAGHGGM
                     DFFVINAFIECIKQKTEFPLDVYDLATWYAITPLSEKSIEGGGKLMNIPDYTRGKWKT
                     RKPVFCIKEDGNY"
     gene            complement(7394..8434)
                     /locus_tag="PLU36_02840"
     CDS             complement(7394..8434)
                     /locus_tag="PLU36_02840"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HMZ45720.1"
                     /translation="MDNQIILEIADKYGWSNIEYNFISSGLINNTAKITAKQGSFILQ
                     QINTGVFATPSTIDNNIQLLKQFVQINNPDYLFTNTIETKQKETLICINNNYYRAFNF
                     IENSYTIESVTHESEAYEAATAFANFTNVFSNFNSDSLQDTIPHFHNLELRFSQFEKA
                     LKIGNQQKIKEHRIIIDEIYSVENICKKYNALLGSKECKKRVTHHDTKISNVLFKQNK
                     AICVIDLDTVMSGYFISDVGDMFRTYLSPVTEEETNLDAICIRKEVLEAIYQGYMDYM
                     HQHLSKYEKQFFYFAGEVMIYMQAIRFITDFLMNDIYYKTSYYHQNLNRAKNQLTLLK
                     AFQSATNNCHLP"
     gene            complement(8456..9548)
                     /gene="prfB"
                     /locus_tag="PLU36_02845"
     CDS             complement(join(8456..9499,9501..9548))
                     /gene="prfB"
                     /locus_tag="PLU36_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793088.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HMZ45721.1"
                     /translation="MTHEKLNDIKERLLVLGGFFDYDNRIAKIEQDKQISLSAGFWDD
                     NKRATSIMKEIKVNEYWVKLFNTVETAIDDFAVLFEFYKAADATEEETLNAFNNAISE
                     LDNAEFKSTLNKPEDELNAVLQINSGAGGTESQDWAEMLLRMYKMYAAKQGWKVTELD
                     FLEGDGAGIKTATLQIDGAFAYGFLKAESGVHRLVRISPFDSNARRHTSFASVFVYPL
                     LDDSIEIEIKDSEVKMETARSGGAGGQNVNKVETKVFLTHIPTGITVICQTERSQIGN
                     REKAMQMLKSRLYEEELRKREELKNATNANKKKIEWGSQIRSYVFHPYKLIKDHRTDF
                     EVGNVGPIMDGELDGFIKAYLMISKEEEN"
     gene            complement(9600..10088)
                     /locus_tag="PLU36_02850"
     CDS             complement(9600..10088)
                     /locus_tag="PLU36_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HMZ45722.1"
                     /translation="MRKLILVFLICFFHLSANSQTVQKVTINDVVNMMDTATVPTVIN
                     FWATWCGPCVKEIPWFEKLVEEYKKNNINIRLILVSLDFSTAYPNEISDFVKKKNFTS
                     KIVWLNETNADFYCPLIDKNWTGNIPVSIMVNNGTKFKEFYNSQLPEPRLRLELQKLI
                     AK"
     gene            complement(10112..10708)
                     /locus_tag="PLU36_02855"
     CDS             complement(10112..10708)
                     /locus_tag="PLU36_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HMZ45723.1"
                     /translation="MTPERTEKLLNVLSRRQHNLTVVMENVQDPHNISAVMRTCDAVG
                     IQDIYILNTKIPRHKKFGAKSSSSAAKWLTVHQFDNVQDCFNELRTKYNKIFTTHLST
                     GAVSLYEINFTENIALVFGNEHSGVSEEVRSLADGNFIIPQMGIIQSLNISVACAVSI
                     YEAFRQKNNAGHYLKSTMPQAQRNNLLKNWGFIEHDNN"
     gene            complement(10723..11295)
                     /locus_tag="PLU36_02860"
     CDS             complement(10723..11295)
                     /locus_tag="PLU36_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HMZ45724.1"
                     /translation="MFLFFSKEIKAQGKYDTIKVYAYITAYGDTIPMSFLPPVEVSTK
                     LSRAARRHLTEWNRLRKAVYVTYPYAIAASRIMNEVNAKLVGVENKKQRKKIIRAREK
                     ELKKEFADKLTQMSVYQGKVLMKLINRQTGNNCYEIIDEYKGSFTAAFYQTIAIIFGS
                     NLKQPYDAKGKDADMEKIVIDVEKMYGYRS"
     gene            11641..12624
                     /locus_tag="PLU36_02865"
     CDS             11641..12624
                     /locus_tag="PLU36_02865"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HMZ45725.1"
                     /translation="MKKCIAIVTGGYSKEAEISYKSVITVTNNIDKQKFDWYIIDINP
                     EGWWYLNDANEKIFVNKNNFTIDVNNTTITLDAVLMCMHGTPGEDGKLQGYFDMIGLP
                     YTSCNAAVSALTFNKKFTVSVAANSGINVAKSIMLFKNNSINEKEIAQQLKFPVFVKP
                     NNGGSSIGMSKVYETSNLSAAISKAFEEDDQILIEEFIAGRELTIGVFKTKGEIITLP
                     ITEIITKNDYFDYKAKYLGESDEITPANVEETIAEKIRNAAKKAYQVFNCNGIVRIDF
                     IFESKLQQPFMLEINTVPGQSAESIVPQQVKAMGWSLQDFYTALIEECFTK"
     gene            12685..13503
                     /locus_tag="PLU36_02870"
     CDS             12685..13503
                     /locus_tag="PLU36_02870"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HMZ45726.1"
                     /translation="MKLFKFITDKPLWVNIVVAITLLMVLIFIFFISLKSITSHGESI
                     KVPNTLGQNITAAQKMLESQGFSVEVLDSVFIDTIARLSVFKQSPEADVVVKKGRTIY
                     LTVNKAIAPEVEMPSLIGYSYKSAELMLQSLSLKMGDTSYKADFARNSVLEQYYNRKP
                     IKEGTKIPMGSTISFVLGSGVGNTDINVPNLLGLKVSEALGILQNLHLNVGPIIAQEA
                     ISDTLNAFVTEQRPPAFSEPTPGQKVQNKLRAGQVIDLFIRNTPITVQDSTITQ"
     gene            13518..13826
                     /locus_tag="PLU36_02875"
     CDS             13518..13826
                     /locus_tag="PLU36_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMZ45727.1"
                     /translation="MQNITVEELKERLDTGNPINLIDVREETEHADFNIGGINFTLRR
                     MQDMAIEDIEHLKNEEVICYCRSGQRSNMACLILEYQGFTNTINVIGGVNAWQEKFGR
                     "
     gene            13965..14270
                     /gene="rplU"
                     /locus_tag="PLU36_02880"
     CDS             13965..14270
                     /gene="rplU"
                     /locus_tag="PLU36_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HMZ45728.1"
                     /translation="MLAVVKIAGQQFKVQAGDSLYVPHLQGSTGDKVEFNEVLLVDNA
                     GTVSAGSNAKATVKAEIIKSLVQGDKVIAFKMKRRKGFRKKHGHRTHYTQIKIESIA"
BASE COUNT         4995 a         2169 c         2056 g         5114 t
ORIGIN      
        1 ttaatagtgc caattaatta tttaatattc tataaccact tgtaggtata tcaaactttg
       61 aagaagacac aggatttaag ttaatttttg ttgcaaaata tttaattttt gcaccgcttt
      121 tatcttgcga ttcatattct aacacaaagc caggaacatt tttaaattga aattcaaatt
      181 cacttactga tggtataaaa cctggtgtat aatataagtt taacgataca ctatcggcta
      241 acattaagat tgcttttttg cattcgtatc ctaatataat tttagtttca tttaataaag
      301 taagttgtgc attctcaaat ctgctattag attttttcca agctaggcta tctaatttag
      361 ttaaaaattt attgccacct atttctctta aaattacagc atcgccagtt cttttattgt
      421 aaataacaga ttgagaaaat gaagtactat taaaattcac cctactttgg tagcctttta
      481 tatataaagt tttactggtt gatttaaagg tttccaatgt agcattatct atacctttta
      541 catcatctgt atttactgca aattctacag tacattctgc aataacacgc tgcctttgct
      601 gtgctaatag tgtgttaaca gaaagtaaaa aagtagttag tattatcaaa gtacttttca
      661 ttttagtttc tttttatgaa tgcttaaata actatataca atattatgcc aatgtttaca
      721 aaaaagctcc tgttttatag gagcttcatc aattttattt tttagtctta ttcttcattt
      781 cctgaatttt cttttggttt tctaccattt gagtataacg ttcttgccat ttactctgct
      841 ttttaggatt acttctttta gcttcaattt ttgctaaaat tttatcatgg tcaataatat
      901 atttttgaat aataaattgt aaaattaacg taaccaggtt agatactgta taataccaag
      961 ttaaagctgc tggcatttta ttgaatacaa agaacaatac aaacggaaaa atataaggca
     1021 tgtattttag agcaggatta tcttgagttg gtgtattacc catattatat atagatatta
     1081 aaaaactggt aataacagca gtaatattaa acaaactaat atggttgcct actaaccaaa
     1141 tattagtacc ccaactaatc acatcatcat aagaagataa gtctttagcc cataaaaagt
     1201 tttcgcccct tagtgcaata tttgagttaa agaaactaaa taaagcaaag aatataggaa
     1261 tttgcaatag tgctggaata caacctccta aaggatttac acctgcttct ctaaacagct
     1321 tcatttgctc cattgcaaat gcttgtttat cattttctcc aattttcttt tttagagcat
     1381 ctaattctgg tcttaatact ttcatttttg caccacttaa gtaactacta taagtaagcg
     1441 gtgcagttat taaacgtata aataaagtta gcaataaaat tacccaacca taattaccta
     1501 taaagctaga aaagaaatta aaaacaggca tgatgatgta ttggttaata ggacgaacaa
     1561 atgaatacat atctcttccc aaattcacaa ttctgtccat ctcaggagct tgtttcttta
     1621 aaatattgta atcattaggg ccaaaataca tttttaaagg aatagtgtta gtagctgcag
     1681 tagttatttt aatttgaaaa ttagcttgaa catttgctaa aatttttgta gtatctgttg
     1741 tatgtttttc ccattttaca ttaccagagt tgaagttatt ttctgcaact aacgttgtgt
     1801 taaaaaattg ctgcactaca ctcatccagt tagttggttt ttcaaaagta tgttccgttt
     1861 tgttcatgat gtagtcaaac tctttgtttt ctaagaaaca aatattactc atttgcactc
     1921 tttcgtactc tgcacttttt tcgtgttgtg tagtttgagt ttgccaacta atatttaggt
     1981 tgccatcatt taataactgg ttagctgcag ttaaactaac tttccagtca atcatatacg
     2041 tattaggtgc aatggtaaaa atatgttgta tatttctacc agcagaatct gctaactcat
     2101 aaataatagt ttgagagcca tcggtatttt tttgattaga ttttaattga aaatataaat
     2161 cgttggtgtt agctacagca ttattacctg tattaatatt atagcctaat ttatcgtttg
     2221 ctgttcctaa aacaactggt gtactatcgt aagaattata tttttttaaa cttacagttt
     2281 gaattctgcc acctttattg gtgaagttaa tttttaatat atcagtttca atagtttcga
     2341 ttttttctga accatttgca gagccagtaa agttgccttc ttttaatatt ttgttggtag
     2401 aatctctttt aactgaatct tcaatagctt tagcttttat ttctggggtt atttttgaag
     2461 cctccacctt ggctaaagaa tctttcacct tgttttcatg atcaatggca ctttgagcca
     2521 tcttgttatt gtaccaaaaa aaagcaataa ataaaattcc taaaagtaca aaaccaataa
     2581 tcgtattctt atctgtcttc attgtctgtt tttaaaagat tggcaaaggt agggaactga
     2641 aagtgaatgt ggcaatatgt gtttggaact attttattac taataacata ctgatttgca
     2701 tttatttatg ccaataacag atttaatgaa ttgttttaat ttaaaacaaa taaattttag
     2761 tactaattaa ctttcgggca aagcatacca tttacgatac attttaaaaa taaatgtagg
     2821 aataattaaa agaataagta ctaccaacac aatttcagaa atagaaagat ataaattaat
     2881 tgttttcatt ttttcctgat aattaatatc gttaattata taatttaacc ttgccctttc
     2941 attgtaataa aatacaaaca ttaaaggtaa aaatgcaaaa attaaggtaa ataaatttat
     3001 agcaacaccg gttactactt ttcttgaaga ctgtgtaaac accattattg aaagaaataa
     3061 gaaaaggatg acataaaaga aataccattc ataaactgca tagcctttaa aatcaaacaa
     3121 tgctattact agccctgaaa tgatggttaa taattttgca gtaagtaatg atagaaaaaa
     3181 tgtttttact gtactatgtc taaatatata aaccaataaa cttaaaataa gagtaaaata
     3241 taaaacaact cttaaaatat ctttcatctc ttcaaaagcc catctgtttt taattctaaa
     3301 aatattttct atacttcttt ctaccgacca taaattatat tttttaaaaa tttcattggt
     3361 gcttagtaaa tggctattgt taccttgctc atcaaattca gtgtttatac tactttggta
     3421 gttgttaatt gaatacttat ttaaaatagt ttttaaaagt gattgtaaac tatcttttgg
     3481 tgcaggttta taatttttta ttaccaaatt aaagatagaa gcagatttta aaattttatc
     3541 ttcaccaata tcttttgcat tataaatatt taaaaaagaa tacaccgggc actctacaaa
     3601 tacatataaa ttattgccta aatcaatcac actatcagtt attttttttc ggctattaaa
     3661 agttgcagaa tcaatagctg cgtatttaga atgttgagtt atttttggta tagaatctct
     3721 ttgttgtgta tctatcttat tcaaatctgc aaccaaccaa actgtgtctc tgttccaatt
     3781 atgttgtatg gaatcatatt caatttgtgc aatagttaaa ttaattttat taatatcatt
     3841 aatcaattct ttagaattgt atgtagaatt tacccttaca acttctacaa cagagaaaac
     3901 aaaagggaaa gtcatgaaac ataaaataca ggtataataa agtagtaatt gttttaagct
     3961 atctaatgca gtgaagtttc caaaacgttt aaaaacatta aattgaagta aataaattag
     4021 ccaaataaca aaagaaataa aactaataat agatacaaag actatccaat aaccattaac
     4081 cgaatctctt ctaaactcta gtggaactac aaatgatatt atactaagca aaataataaa
     4141 taaaatactg taccaaatta cccaatgaat tttggtgctc catattgttg gattattttt
     4201 taataacctc tcgtctaatt ttagtaaaaa tgaaggctta aacttttgta tatctttcat
     4261 tacttgcatt ttaactgaac aatctttttg tagagttagt aatatctcta aaataagtaa
     4321 tggcaatttg ctgtgctaac aacttctcca taataacatt ggcagttaat tgattagaag
     4381 ttatagtata ataaccacca ttataactta cctgaacatt attttcaaga atttgtaaaa
     4441 tagtatctct gttagcattg gtttctattt caatcaccac ttctttgtgt gtaacattgt
     4501 tgctgcatgt agtttgaaat atgcagttac catttttaat aaataatatc tcatctgcta
     4561 ctttttcaac ttcgtgtaat tgttggctac ttaatataat gcttaaagga taataaatag
     4621 atttagctaa atatttaaaa tctattagca tggtttgttg tgcattaata tcaaggttgg
     4681 ccagtggctc atctaaaata agtaaggtag gtttttgaag taatactctt gcaatttcaa
     4741 accttgttct ataaccacta ctaatttggc tccaagtaag gttggcatag cttgttagcg
     4801 ataaacgctc taacataaaa tcaaccatta aattattttc tttgctataa ttacctgtaa
     4861 tagatgcaga aaaatgtaga ttatctttta gttttccata ccatttagga atgcgttggg
     4921 gtataaaagc tactttttgt tttatggaaa aataatctgg cttaggatca aagtaataat
     4981 caattgagcc ttcatttatt tgtaactgcc cagctaaaca acgcaataaa gttgttttgc
     5041 catgaccatt ttcgccaacc actccaataa ttttattggt atatgctttt aatgaaagtg
     5101 cttgtaaatt aaacccacct acagtgtacg atttgcttat tttatttgca gaaattactt
     5161 gctcattagt atgaattact gaagaactta gacttgaaaa ttgttgtaaa atttgttctg
     5221 caccatcttg aaatttatct gaaaaattgg ctaaattttg ctctgcttta ttaccaacct
     5281 gcttactaaa gttaattgat tgctgtatta aactctcctt tctggtatct aaacaggcat
     5341 ctaataaacg tcttcttgct aaatcaaaat ctttatactc aataaaagtt cttatttctt
     5401 ctactctatt tttcatagcg gtacattttt aatcaatatc gcccaaagca aacttttttt
     5461 ctggttttaa aaaggcaaaa gacatttgtt tttgtttacc atttaaaagc aaggaaacat
     5521 gcaaaatatg ccataagcct tccgattctt tttcagtttt atattgtaca attttataac
     5581 tatctgcctt gtttattgtt ctatttattc taaaacaaat ttcatccact ccattttttt
     5641 cttcctcaac agagtaatta caataatcct tccattttct tttttcatct ttcccttgat
     5701 aaattatata gggtgccact ttatagaatg tgccatattt tgcaatatca gggtctgcaa
     5761 aatttacatt tttacattcc tttaaaattg tattaaatgt gtttgccact tcggtagagt
     5821 ctttaactct ttgttgagag aatgctgtat tagaaactat caataacaca aaaaataaac
     5881 ctttcacaat agataatttt tttagttaaa taattagatg ctagaaattg agaattgcac
     5941 aatctataaa attagacaat ttaaaaataa tacatttgct aataaataat tattttacat
     6001 ctcacataat taatacacca atttatgaaa cctatgttaa gaagaaagtt cttacaacaa
     6061 acaagtatat taactattgg cagcattgct gcaccacaaa taatttttgg tgctcaaaaa
     6121 ttagaaatta aaaaaataag ggttggctta attggggttg gcttaagagg tcaaaatcat
     6181 ttagatgttt tattgagaag agaggatgta ttggtaacag cattggccga ccctcaacca
     6241 aaaatgcttg caatggccaa tgaattaatt gctaaaagtg gcaaaccaaa acctttaatt
     6301 tttagtaatg gtaattacga ttataaaaat ttattgaaaa gagatgatgt agatgcagta
     6361 attatttcaa caccttggga atggcatgca gagcaaacaa tagatagctt attagctgga
     6421 aaaatacctg gagtggaagt atctggggct gttaagttag ctgaatgttg ggatattgta
     6481 aatgctgcag aaaaaaccgg attgcctgta atggttttag aaaatgtttg ttatagaaga
     6541 gatgtgatgg ctgttttaaa tatggttaga aaaggaatgt ttggcgagtt gttgcatgca
     6601 caaggaggct accaacatga tttaagaggt gtgaaattta atgatggaat tactccgtat
     6661 aactctggtg tagaatttgg agaaaaaagc tttagtgaag ctgcttggag aacacaacat
     6721 agcataacca gaaatggaga actttaccca acacatggtt taggacctgt agcagtaatg
     6781 atggacatta atagaggaaa taggcttacc aaactttcct ctatagctac aaaagccaaa
     6841 ggcttgcatc gttacattgt aaaccaccct aagggtggcg aaaaccatcc taatgcaaaa
     6901 attaaattta aattaggaga tattgtaaca acacaattgc aaactgcaaa tggcgaaacc
     6961 atagttttaa cacacgatac aaattctcca cgcccctaca acatgggttt tagagttcaa
     7021 ggcaccaatg gtatttggca agataaccat gcaggtgagt ttactgcagg cttaatttat
     7081 tttgaagata aaagcaaacc acatagatgg gaaaatacag aacaaaatat gaaaacatac
     7141 gatcacccat tatggaaaaa atatgaaaaa gatactgcag gtgccggaca tggtggaatg
     7201 gatttttttg taataaatgc ttttattgaa tgtatcaaac aaaaaactga atttccactt
     7261 gatgtttatg atttagctac ttggtatgct ataacccctc tatctgaaaa atctattgaa
     7321 ggtggaggaa aactaatgaa tataccagat tatacaagag gtaaatggaa aacaagaaag
     7381 ccagtttttt gtattaagga agatggcaat tattagtagc agactgaaat gcttttaata
     7441 aggttaattg atttttagct ctgtttaaat tttgatggta gtagcttgtt ttgtaataaa
     7501 tatcgttcat taaaaaatca gttataaacc ttattgcttg catgtaaatc ataacttcgc
     7561 ctgcaaaata aaaaaattgt ttttcgtatt ttgaaagatg ctggtgcatg taatccatat
     7621 atccttgata tattgcctct agtacttctt ttcttataca aatggcatcc aaattagttt
     7681 cttcttctgt tacagggctt aaataggttc taaacatgtc tcctacatca ctaataaaat
     7741 aaccagacat tacagtatct aaatcaataa cgcaaatagc tttattttgt ttaaacaata
     7801 cattgctaat ttttgtatcg tggtgtgtaa ctcttttttt acattcttta gaacctaata
     7861 gagcattgta ttttttacaa atattctcta cactatatat ttcatcaata ataattctat
     7921 gttctttaat tttttgttga ttgccaattt tcaatgcttt ttcaaactgc gaaaacctta
     7981 gctctaaatt atggaaatgg ggtatggtat cttgtaagct atcagaatta aaattagaaa
     8041 acacattggt aaaatttgca aatgcagttg cagcttcata agcttcactt tcatgagtaa
     8101 cactttctat tgtgtaagaa ttttcaatga aattaaaagc tctgtagtaa ttattattta
     8161 tgcaaattaa agtttctttc tgcttagttt caatggtatt cgtaaataaa taatctggat
     8221 tatttatttg tacaaattgt ttaagtaatt gtatattatt atcaatagta gatggagtag
     8281 caaaaacgcc tgtgtttatt tgttgcagaa taaaacttcc ttgcttggct gtaattttag
     8341 ctgtattatt tattaagcca gatgaaatga aattatactc aatattagac cagccatatt
     8401 tatctgctat ttctaaaata atttggttat ccattatttc gtcaattaaa tttctttagt
     8461 tctcctcttc tttgctaatc attaaataag ctttaataaa gccatctaat tcaccatcca
     8521 ttataggtcc aacattgcca acttcaaaat ctgttctatg gtcttttatt aatttataag
     8581 ggtgaaaaac atagcttcta atttggcttc cccactcaat cttttttttg ttggcattag
     8641 ttgcattttt cagttcttct ctctttctta attcttcttc atacaatctg cttttaagca
     8701 tttgcatagc tttttctcta ttcccaattt ggcttctttc tgtttggcat attacagtaa
     8761 tacctgtagg tatatgggtt aaaaaaactt ttgtttcaac cttgtttaca ttttgaccac
     8821 cagcaccacc gctacgtgca gtttccattt taacttcact atcttttatt tcaatttcta
     8881 tagagtcgtc aagcaaagga taaacaaaaa ccgaagcaaa gcttgtatgc cttctagcat
     8941 tactatcaaa tggcgaaatt ctaactaacc tatgaactcc gctttctgct tttaaaaatc
     9001 cgtaagcaaa agcaccatct atttgcaaag tagcagtttt tattccagcc ccatcccctt
     9061 caagaaaatc aagttcagta actttccagc cttgttttgc agcatacatt ttatacattc
     9121 ttaaaagcat ctctgcccaa tcttgacttt cggtaccacc agcaccagag tttatttgta
     9181 atacagcatt taactcatct tcgggcttat ttaaggtact tttaaactct gcattatcca
     9241 gctcacttat agcattatta aaagcattaa gggtttcttc ttctgttgca tctgcagctt
     9301 tgtaaaattc aaataaaaca gcaaaatcat caatagcagt ttctacagta ttgaataatt
     9361 taacccaata ctcattcact tttatttctt tcattataga agtggctctt ttattatcat
     9421 cccaaaagcc tgcagagaga gaaatttgtt tatcctgttc tatttttgca atacgattat
     9481 catagtcaaa gaaacctcct caggacaagt aagcgttcct ttatatcatt taatttttca
     9541 tgtgtcataa catggcaaaa gtaagtgtat agctatttaa gatactaata atagaaaagt
     9601 tattttgcaa ttagtttttg caattctaat cttaaccttg gctctggcaa ttggctattg
     9661 taaaattctt taaactttgt accattatta accataatac ttacaggaat attaccagtc
     9721 cagtttttat caattaaagg acaatagaaa tctgcattag tttcgtttag ccaaacaatt
     9781 ttacttgtaa aatttttctt ctttacaaag tctgaaattt cattaggata agcagtacta
     9841 aaatccaaac tcactaaaat taatcttata ttgatgttat tttttttata ctcttctaca
     9901 agtttttcaa accaaggaat ttctttaaca caaggcccac accaggttgc ccaaaagtta
     9961 ataacagtgg gtactgtagc tgtatccatc atatttacca catcgttaat agttactttt
    10021 tgaactgttt gactatttgc agaaagatga aaaaaacaaa ttaaaaaaac aagtattagc
    10081 tttctcatat tttataattg tatgttgcaa gttagttatt atcgtgttca ataaagcccc
    10141 agttttttaa taaattattt ctttgagcct gtggcattgt gctttttaaa taatgcccag
    10201 cattgttttt ttgcctaaat gcttcgtata tacttactgc acaagcaact gaaatattta
    10261 agctttgaat aatacccatt tgtggtatga taaagttacc atcagctaag cttctaactt
    10321 cttctgatac accactatgt tcattaccaa aaactaatgc tatattttca gtaaaattta
    10381 tttcatacaa acttactgca cctgtagata aatgagtagt aaatatttta ttatactttg
    10441 ttcttaattc gttaaaacaa tcttgcacat tatcaaattg atgtacagta agccattttg
    10501 ctgcactact gctacttttt gctccaaact ttttatgtct tggaattttt gtatttagaa
    10561 tataaatatc ttgtattcca acagcatcgc aagtacgcat tactgcagag atgttatgtg
    10621 ggtcttgtac attctccatt actactgtta aattatgctg ccgcctactt aatacattta
    10681 gtaatttttc tgttctttct ggtgtcatta aaaaaaattt acttagcttc tgtatccata
    10741 cattttttct acatcaatta caattttttc catatcagca tctttaccct tagcatcata
    10801 gggttgcttt aaattacttc caaaaataat agctattgtt tgataaaatg cagcagtaaa
    10861 actgcctttg tattcatcta taatttcata acaattattt ccggtttgcc gattaattaa
    10921 tttcattaaa acttttcctt gataaaccga catttgagtt agtttatcag caaattcttt
    10981 cttcaattct ttttctctgg ctctgataat tttttttctt tgctttttat tttccacacc
    11041 aaccagctta gcattaactt cattcattat tctacttgct gcaatagcat aagggtaagt
    11101 aacataaact gcttttctaa gcctattcca ttcagttaaa tgccttctag ctgctctcga
    11161 aagttttgta ctcacctcta caggtggtaa aaaactcata ggaattgtat cgccataggc
    11221 tgtgatatat gcatatactt ttattgtatc atatttacct tgtgccttta tttctttaga
    11281 aaaaaataaa aacaaagaaa aaataaggag aaagatagta ttttttttat gccactgcat
    11341 aatattaaaa gtacaattta ttatattaat acgttttaaa cttaaaagta gccctagttt
    11401 ttttatactt aacaaaattt gaacatttta tagaggcctt cttaatattg ttttactaaa
    11461 ttttcattaa ttattaattg ttacccaaaa aacaactaat ttttaatatt actttgtaac
    11521 tattaatttc aacaaaaaat taagtaccat taaaattact tattaaatag aatattttta
    11581 aattatttct gtactataaa taatttaaaa aaattaaaaa gaagtattaa aaataaaccc
    11641 atgaaaaaat gtattgcaat tgttacaggc gggtacagta aagaagcaga aattagttat
    11701 aaaagcgtaa ttacagtaac taataatatt gataagcaaa agtttgactg gtatataata
    11761 gacataaacc cagaaggatg gtggtatttg aatgatgcaa atgaaaaaat ttttgtaaat
    11821 aaaaataact ttacgattga tgtaaataat acaactataa cattagatgc agtattaatg
    11881 tgcatgcatg gaacaccagg agaagatgga aagctacaag gatattttga tatgattggc
    11941 ttgccatata caagttgtaa tgcagcagta tctgcactca catttaataa aaaatttaca
    12001 gtatctgttg cagcaaatag tggaataaat gttgcgaagt ctatcatgtt atttaaaaat
    12061 aattctataa acgagaaaga aattgctcag caactaaaat ttccggtttt tgtaaaacct
    12121 aataatgggg gcagtagtat aggcatgagc aaagtatatg aaacaagtaa cctatctgct
    12181 gctattagca aagcttttga agaagatgat cagattttaa ttgaagaatt tattgctggc
    12241 agagaattaa caattggtgt gtttaaaaca aaaggtgaga ttataactct tccaattacc
    12301 gaaataatta caaagaatga ctactttgat tataaagcaa aatatttagg agaaagtgat
    12361 gaaattacac cagccaatgt agaagaaact attgcagaga aaataagaaa tgcagccaaa
    12421 aaagcttacc aagtatttaa ttgcaatggc atagtacgta ttgattttat ttttgaaagc
    12481 aaattacaac aaccttttat gttagaaatt aatacagtac caggccaaag tgcagaaagt
    12541 atagtaccac agcaggttaa agcaatgggc tggagtttac aagattttta tactgcattg
    12601 atagaggaat gttttaccaa ataattcaat ttattacaat caacatcata atttttctac
    12661 atttttgtat tgaattttat ttaaatgaaa ttatttaagt ttataaccga taaaccactt
    12721 tgggtaaata ttgttgttgc aataactttg ttgatggtat taatttttat atttttcatt
    12781 tccttaaaat caattaccag tcatggtgaa tctattaaag tacccaatac attggggcaa
    12841 aatatcaccg ctgcacaaaa aatgttagag agccagggtt ttagtgtaga agtgttagac
    12901 tctgttttta ttgacacaat agctcgttta tccgttttta aacaatcgcc agaagcagat
    12961 gtagttgtta aaaaaggaag aaccatttac ttaacagtta ataaagcaat tgcacccgaa
    13021 gttgaaatgc cttcgctaat tggatactct tataaaagtg cagagctaat gttgcaaagt
    13081 ttaagtttaa aaatgggtga taccagttac aaggcagact ttgcaagaaa ttcagtgcta
    13141 gaacaatatt acaatcgtaa gccaattaaa gaaggaacaa aaataccaat gggcagtacc
    13201 ataagttttg tattaggaag tggtgttggt aatactgata ttaatgtgcc taatttactt
    13261 ggcttaaaag tttctgaagc attgggtatt ttgcaaaatt tacatttaaa cgtaggacca
    13321 ataattgcac aagaggcaat atctgatacg ttaaatgcat ttgtaacaga gcaaaggcct
    13381 cctgccttta gcgaacctac acctggtcag aaagttcaaa acaaattaag agccggacag
    13441 gttatagatt tatttattag aaatacacca ataactgttc aagactcaac cattacacaa
    13501 taatataaat ttattttatg caaaacatta cagtagaaga gcttaaagaa agattagata
    13561 ctggtaaccc tattaattta atagatgtta gagaagaaac agaacatgct gattttaata
    13621 taggaggaat taattttact ttaagaagaa tgcaagacat ggcaatagaa gatattgaac
    13681 atttaaaaaa tgaagaagta atttgttatt gtagaagtgg acaaagaagc aatatggcat
    13741 gcttaatttt agagtaccag ggttttacaa ataccattaa tgttataggc ggagtaaacg
    13801 cctggcaaga aaaatttgga agataaggct tgaattatag caccctaaac catttatttt
    13861 caggttattt ccttaataat tagccttgaa aaataaaata tgcaaaaagt ttgctgaaat
    13921 tgagcaaata ttcttacttt tgccgtccta atttttaaaa agtcatgtta gcagtagtaa
    13981 aaattgcagg tcaacaattt aaagtgcaag ctggagacag tttatatgta ccgcatttac
    14041 aaggaagcac tggagataaa gtagaattta atgaagtatt attagtagat aatgcaggaa
    14101 ctgtttctgc aggtagtaat gccaaagcaa cagtaaaagc cgaaataatt aaatctttag
    14161 ttcagggcga taaagtaatt gcttttaaaa tgaaaagaag aaaaggcttt agaaaaaagc
    14221 atggtcatag aacacactat acgcaaatta aaattgaaag tatagcttaa aaaaataaaa
    14281 ctaaaagcaa aaaataaaat gctttttaat tttataaagt aattagtttt taaa
//
LOCUS       DAOQPK010000034        13960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_2254_length_13960_cov_5.049407, whole genome shotgun sequence.
ACCESSION   DAOQPK010000034 DAOQPK010000000
VERSION     DAOQPK010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13960
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2254_length_13960_cov_5.049407"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..1956
                     /locus_tag="PLU36_02885"
     CDS             79..1956
                     /locus_tag="PLU36_02885"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ45729.1"
                     /translation="MIFRYILLLIICSTYCSTHLLSQCNTTISTFPYHEDFEAGQGNW
                     TTGGNNNDWQYGTPSKSIITGAASGTKCWIVGKLTGNFYNFGENAWIMSPCYDFSSLT
                     NPQISFSVFWETERKYDGATFQYSVNGGNTWNTLGSTNSNNNCNGVNWFNYSPITSLG
                     VDGWSGTKLPTSGSCNGTGGSDGWVTSKHILTGLAGQNNVRFRFVFGAGTTCNSFNGF
                     AVDDITISEAPVNTCNFIFSCTGNNTVNFTNTSSICATNFNWNFGDNASPSNTSNAEN
                     PTHIFTAPGTYNVSLTVTFPGNIIVNKIKTVTVLSVNTSIVNSIKCNGDSIGSALAIV
                     TGGGGVYNYNWNTNPVQTTNTATNLKAGSYTVTVSATNACTTNSTIIFTQPDSLKATS
                     IVANELCKQQNGFIEVVTTGGVLPYTYLWNTNPIQNTSKASNLKAGNYAVIITDANLC
                     KLSMNNSIKDSINNINLNLGNDTSFCPGSQLILNAGAGFANYVWQNASTNATFTATQT
                     GNYFVIVTDKDGCTKTDTIKINVDCSDIYFPTAFTPNSDGLNDTFGALGNLFSLTSYQ
                     FYVYGRWGQLIFYTNNAFEKWDGKQNGNNLDSGTYIWFATYSINNKPLLFKKGTVIMI
                     R"
     gene            2007..2078
                     /locus_tag="PLU36_02890"
     tRNA            2007..2078
                     /locus_tag="PLU36_02890"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2040..2042,aa:Arg,seq:cct)
     gene            2164..3066
                     /locus_tag="PLU36_02895"
     CDS             2164..3066
                     /locus_tag="PLU36_02895"
                     /inference="COORDINATES: protein motif:HMM:NF015272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HMZ45730.1"
                     /translation="MSKENILESIDKKYADLGENAETYLKGLLQAKPITYWDYIQVDT
                     LHSLQKPRTNYKDEEIFIMYHQVTELVLKMMVHELKQLVFEEHTEEIYIDKVNRLNRY
                     TSMLITSFDVMKYGMNYDDYNIFRSTLTPASGFQCAQFRYIEIFCTRLENLINEEGKK
                     RLGPNPSVEDYFEHIYWKDAGLNRQTGKKSLTLQLFEEKYLSSFIELAKKVQGNTLEE
                     KINKLPNVSEAFTKKMKEFDHLYNVEWPIVHLQTAQHYLDSKGENKAATGGSEWKKYL
                     HPQYQQRKFFPTIWSEEEKRNWGK"
     gene            3082..3363
                     /locus_tag="PLU36_02900"
     CDS             3082..3363
                     /locus_tag="PLU36_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45731.1"
                     /translation="MNTSLDILQIENYTKEDLLKFINNLIHHDFEKLVFLLYRIDVAE
                     EKIKNLLSNNHTTNVEELIANAIIERVMEKKISKEKYKQQNHISEEEKW"
     gene            3436..4446
                     /locus_tag="PLU36_02905"
     CDS             3436..4446
                     /locus_tag="PLU36_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HMZ45732.1"
                     /translation="MKLVSYLKDGHDQLAFLVDNFLFDCDMVHPELPGNMNMFLHYWD
                     DMYPIAQKVDAAIKAGSISKDRGIDISTIQIIAPIPFPTSCRDGYAFRQHVAAARRNR
                     KVDMIAEFDQYPIFYFTNHLSIQGEGNIYCMPDHFEKLDFELEAAIVICRPGRNIPAE
                     EADKYIGGLMIMNDMSARRLQMEEMLLNLGPAKGKDFCTVIGPCLVTLDELEAFEIPA
                     KENHVGKSWNLKMKCWVNGIQVSEGNVGDMDWTFAEIIERCSYGVNLHPGDVIGSGTV
                     GTGCFLELNGTGKLNNPNYQEQWLQENDVVEMEIEQIGLLKNTIVKEESSFSILAKKK
                     LP"
     gene            4494..6404
                     /locus_tag="PLU36_02910"
     CDS             4494..6404
                     /locus_tag="PLU36_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HMZ45733.1"
                     /translation="MSTPLQILQQYWHHQTFRGEQEKIIEAVLNKNDVVALLPTGGGK
                     SVCFQVPAMLMDGLCLVISPLIALMKDQVENLTKKNIPAAAIYSGMSFFEINKILQSA
                     TDNNLKFLYISPERINTKLFQQCIHQLNICLVAVDEAHCISQWGYDFRPSYLKIAKIK
                     EQLNSVPFIALTATATTVVKNDIIDKLQLKNVIVFKQSFEKSNISFSVFNVDSKINKA
                     VEILNKVQGSSIIYCKNRNETKAIAEQLLKQNISANFYHAGLTQEERSVRQNNWIKNK
                     SRVIVCTNAFGMGIDKPDVKTVIHFNIPDCIENYYQEAGRAGRNGSKAYAVLLYQLSD
                     EIELDTLAEVKFPAIETIKEVYQHIANYLQIPIGIGEGNYYNFDIIDFCNNFKLDVVQ
                     VINILKYLEQEGFFAFSENIFIPAKVVFTSNKQTLNSVENRYPHLDLVMKTLLRTYEG
                     IYENNVSINEKLIAKIARLSYDTVFSNLNTLHTLGIINYMPQKETPQLYYMLNRAPAN
                     GLFINYSTYLQRKKLYKEKIEAIKQYVHLAETCRSKFIANYFGDKNVSNCNCCDNCLN
                     LKKNKLSLKEFDQIEKLIYRVLSNNELPLNTLLQQLSNFKKEKVLQVITFLETEKLVS
                     TSSTGIITLNKL"
     gene            complement(6399..7769)
                     /locus_tag="PLU36_02915"
     CDS             complement(6399..7769)
                     /locus_tag="PLU36_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMZ45734.1"
                     /translation="MKEQLQNLRNYFNTGATKKYLFRIEQLKKISFALKKYEKEILIA
                     LHTDLGKCEAEAYASEIAIVQNEIKFFIKQLKRLMQPKEVSTNLMNLPGKSKIYYEPL
                     GIVLIIAPWNYPFQLAFNPLVGAIAAGNCVVVKPSEFANATSLIMEKIVKEIFSEDYI
                     YLVQGDGAQIVPTIIEEGSINHVFYTGSTSVGKLIYEMAAKKLIPVTLELGGKSPCVI
                     EDDANLKIAAKRVVIGKFINAGQTCIAPDYLLIHKKIKAKFLEILISTIQSFYTNNAA
                     LSKDYGKIINEKRFNTLISFLQQGKIIYGGKYDKESLYIEPTIMQEIPTNAPINHEEI
                     FGPILPIFDFETQSEAVEIIKQNATPLAFYIFTSSTTKEENWINAIPFGGGCINNTAY
                     HFTNHHLPFGGIGLSGIGSYHGKKSFYTFTHAKPVLKSATWFDPNIKYPPFKGKLRWL
                     KMILKL"
     gene            7835..8311
                     /locus_tag="PLU36_02920"
     CDS             7835..8311
                     /locus_tag="PLU36_02920"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HMZ45735.1"
                     /translation="MEDILYTDDYFMQQALKEANKAFNEDEVPIGAVVVVNNKIISKG
                     YNQTEKLNDCTAHAEMIALTSAFNFLGAKYIPQASLYVTVEPCLMCCGALYWSKLAKI
                     VYGASDEKSGGLRFFKNNDGEILTSKWPFHPKTKLIKGVLENECEMLMKTFFKLKR"
     gene            complement(8322..8681)
                     /locus_tag="PLU36_02925"
     CDS             complement(8322..8681)
                     /locus_tag="PLU36_02925"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HMZ45736.1"
                     /translation="MKLNTKNIGNLGEDLSAKWFELKGYFILERNWRYKRSEVDIIAS
                     KENKLHFIEIKTRTNSNFGYPEESINQVKMNALKKAAVAYLELNTCWKEFQFDVLSVQ
                     LNKNKDHEFFLIEDVFF"
     gene            8715..9911
                     /gene="manA"
                     /locus_tag="PLU36_02930"
     CDS             8715..9911
                     /gene="manA"
                     /locus_tag="PLU36_02930"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HMZ45737.1"
                     /translation="MNDKIFKLKGVVQHYAWGGFSFIPNLLKLKPTNQQPYAEYWLGA
                     HTSAPSVVVNKNISLLELINQNPEKIVGQEVVKQYNGLPYLFKILDVHEMLSIQVHPN
                     KEEAIKGFEKEEQAGIPINAINRNYKDKNHKPEVMIALSEFWLLHGFKEKSEIEQTLT
                     NHLELNLLLPTFKKEGIKGLYKIAMELPQEQVNTILKPIVKKSIFNKSQNLINKHNTE
                     WWIAKFYANNSTENLDRGIFSILLFNIVQLQKGEAIFQAAGIPHAYLEGQNVELMANS
                     DNVLRGGLTPKHIDVPELLKHTLFQSIQPSTIKPQINGNEKTYLCPVPDFSISIISLK
                     VGERYSSHTFSFEILLVIEGAIIIQGLENNIIKQGEAIGIVADTNYTFTATGDAIIFK
                     AFVPQP"
     gene            9987..10550
                     /locus_tag="PLU36_02935"
     CDS             9987..10550
                     /locus_tag="PLU36_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45738.1"
                     /translation="MKINRSVLFTVAIIILTVVVKFICAPILSLSGISTVIAVALFAG
                     MSIHKKQNTFMLPLIALIVSDIIIEVLYQFNWFQFSGFYKGQILNYGLLLLSVLVGWA
                     IKGKSILSIGLGIFAASTIFFILSNLSVWYFGTMYTKDFSGLMLCYESALPFYKNQLI
                     STAIFLPIIMFAYNYFIEKKYRVAVSH"
     gene            10786..11091
                     /gene="rpsJ"
                     /locus_tag="PLU36_02940"
     CDS             10786..11091
                     /gene="rpsJ"
                     /locus_tag="PLU36_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HMZ45739.1"
                     /translation="MSQRIRIKLQSYDHNLVDKSAERIVKTVRSTGAVVTGPIPLPTR
                     KRIFTVLRSPHVNKKSREQFQLCTHKRLLDIYTSTSSTVDALSKLDLPSGVEVEIKA"
     gene            11391..12008
                     /gene="rplC"
                     /locus_tag="PLU36_02945"
     CDS             11391..12008
                     /gene="rplC"
                     /locus_tag="PLU36_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HMZ45740.1"
                     /translation="MKGIIGKKIGMTSIFSADGKQTACTIIEVAPNVVTQVKTIDTDG
                     YNALQLAIGDKKEKHATKAEINHFAKANTPAKQFTKEFRDYSLTKNLGETITIDIFEE
                     GDKVEVVGTTKGKGFQGVVKRHGFSGVGEGSHGQHDRQRAPGSIGGSSYPSRVFKGMR
                     MAGRMGGDRVKTKGLVVLKVFPEKNYILISGSVPGHNNSIVLIQK"
     gene            12031..12669
                     /gene="rplD"
                     /locus_tag="PLU36_02950"
     CDS             12031..12669
                     /gene="rplD"
                     /locus_tag="PLU36_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HMZ45741.1"
                     /translation="MQIDVLNIKGQKTGRVVELPEDVFGAEPNDHVIYLAVKQYLSAN
                     RQGTHKVKTRAEVHGASRKLHKQKGTGGSRKGNIRNPLYKGGGTIFGPKPHGYSIKLN
                     RKVKDLAKISALSYKAKENAILVVEDINLDSPKTKQIAEAMNALNVADKKSILVLGEY
                     NDNVYLSTRNIPNVASTLLADMNTYDIVNADVIVLTENAAKIFSENEVATEA"
     gene            12702..13001
                     /gene="rplW"
                     /locus_tag="PLU36_02955"
     CDS             12702..13001
                     /gene="rplW"
                     /locus_tag="PLU36_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HMZ45742.1"
                     /translation="MKLTEVLVKPILTEKANAQQEKLRRYAFRVNKKANKLEIKKAIE
                     QFYGVTVTNVNTTIAAGKNKTRYTKAGFIEGKKAAYKKAFVTVAEGETIDLYANI"
     gene            13048..13887
                     /gene="rplB"
                     /locus_tag="PLU36_02960"
     CDS             13048..13887
                     /gene="rplB"
                     /locus_tag="PLU36_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HMZ45743.1"
                     /translation="MALKKFKPVTAGTRWKIGNAYAEITTNKPERSLLEPVKKTGGRN
                     AQGRRTMRYIGGGHKKHYRIIDFKRNKQNIAATVVSIEYDPNRTAFIALLNYTDGEKR
                     YIIAPQGLQVGATVISGNEAPPEIGNALQMKNMPLGTMIHNIEMQPGQGGKLVRSAGA
                     SAQLTNKEEKYAVLKMPSGELRKVLINCFATVGVVSNSDHSLETAGKAGANRWKGIRP
                     RVRGVAMNPVDHPMGGGEGRASGGHPRSRTGKYAKGEKTRTKGKGSDKLIIQRRDGKK
                     LTK"
BASE COUNT         5194 a         1948 c         2287 g         4531 t
ORIGIN      
        1 aaaaatacaa taaaataaaa taatcatcat ggttgttatt aaactattta atttgctgtt
       61 tatttcaaca acagaagaat gatttttaga tatattttat tactgataat ctgctcaact
      121 tattgttcta cacatctact ttcacaatgt aatactacaa tttctacttt tccttatcat
      181 gaggattttg aagctgggca aggtaattgg actactggag gaaacaataa cgattggcaa
      241 tatggcactc catcaaaaag tatcattact ggtgcagcaa gtggtacaaa atgttggatt
      301 gtaggcaagt taacaggtaa cttttacaac tttggtgaga atgcatggat tatgagcccc
      361 tgttatgact ttagctcctt aaccaaccca caaatttctt ttagcgtttt ttgggagaca
      421 gaaagaaaat atgatggtgc aacttttcaa tattctgtaa atggaggtaa tacctggaat
      481 acattaggct caaccaattc taataataat tgtaatggtg ttaattggtt taattactca
      541 ccaattacct ctttaggggt tgatggttgg agtggaacaa aattaccaac gtctggtagc
      601 tgtaatggta caggtggtag cgatggttgg gtaacctcta aacatatttt aacaggttta
      661 gcagggcaaa ataatgtgcg ttttagattt gtttttggtg caggaacaac ctgtaatagc
      721 tttaatggct ttgcggtaga tgatattact atttcagaag caccggtaaa cacatgtaat
      781 tttatttttt catgtacagg caataacaca gtcaacttca ccaatacctc ctcaatatgt
      841 gcaaccaatt ttaactggaa ttttggcgat aatgcatctc catcaaatac aagtaatgct
      901 gaaaatccta cgcatatttt tactgcacct ggcacatata atgtttcttt aacagttact
      961 tttcctggaa atattattgt aaataaaata aaaactgtta ctgtacttag tgttaataca
     1021 tcaatagtaa atagtattaa atgtaatggc gattctatag gatcggcact tgcaattgta
     1081 actggtggtg gtggagttta taattataat tggaatacca atcctgttca aacaactaat
     1141 actgcaacaa atttaaaggc aggaagttat acagttactg taagtgctac taatgcatgc
     1201 accacaaatt ctactattat ttttacacag cccgattcgt taaaggcaac ctctattgtt
     1261 gcaaatgaat tatgtaagca gcaaaatggt tttattgagg tagttactac tggtggtgtt
     1321 ttgccttata cttatttatg gaataccaat cccatacaaa acacttctaa agccagcaat
     1381 ttaaaagcag gtaattatgc agtaattatt acagatgcaa atttgtgtaa attatcaatg
     1441 aataattcta taaaagattc tataaacaat ataaacctaa acttaggtaa cgatacttct
     1501 ttttgcccag gtagtcaatt aatattaaat gcaggtgccg gatttgcaaa ttatgtttgg
     1561 caaaacgctt ctactaatgc aacatttaca gctacacaaa ctggaaatta ttttgttatt
     1621 gtaacagata aagatggctg cacaaaaacg gataccataa aaattaatgt tgattgtagc
     1681 gatatttatt ttccaactgc atttacacct aatagtgatg ggttaaatga tacatttgga
     1741 gcattgggta atttattttc attaacaagc tatcaatttt atgtttatgg tcggtggggg
     1801 caattaattt tttatacaaa caatgctttt gaaaaatggg atggcaaaca aaatggaaac
     1861 aatttagata gtggcaccta tatttggttt gccacttatt ccataaataa taaaccctta
     1921 ctatttaaaa aaggaactgt aataatgatt agataatttt ttacttacat ttgttgctta
     1981 ggctcattga aaattgtatg tctaaaggtc tcatagttca acggatagaa taggagtttc
     2041 ctaaactcta gatccaagtt cgattcttgg tgagaccact cataatttta atattaagag
     2101 caacttttat tgccctaatt ttattcttaa taatcaaaac ttaaacttat taatttgttt
     2161 gctatgagta aagaaaatat tttagaaagt atagataaaa aatatgctga tttaggagaa
     2221 aatgcagaaa cttatttaaa agggttatta caggcaaaac ctattaccta ttgggattat
     2281 atacaggtag atactttaca ctcattacaa aaaccaagaa caaattataa agatgaagaa
     2341 atttttatca tgtatcatca ggtaacagag ttagttctta aaatgatggt tcatgaacta
     2401 aagcagttgg tatttgaaga acatactgag gaaatttata tagataaagt aaacagatta
     2461 aaccgctaca cttctatgtt aattacctct tttgatgtaa tgaagtatgg aatgaattat
     2521 gatgattaca atatatttag aagtactctt acgcctgcaa gtggttttca gtgtgctcaa
     2581 tttagatata tagaaatatt ttgtacgagg ttagaaaatt taattaacga agaaggtaag
     2641 aaaagattag gccccaaccc atctgttgaa gattattttg aacatattta ttggaaagat
     2701 gcaggattaa acaggcaaac aggaaaaaaa tctttaactc tacaactttt cgaagaaaaa
     2761 tatttatcta gttttattga actagccaaa aaagtgcagg gtaatacctt agaagaaaaa
     2821 ataaacaaac tccctaatgt gtctgaagca tttactaaaa aaatgaaaga atttgaccat
     2881 ttgtataatg tagaatggcc aattgttcat ttacaaactg cacagcatta tttagatagt
     2941 aaaggagaaa ataaagcagc aacaggtggc agcgaatgga aaaaatacct acaccctcaa
     3001 tatcaacaac gtaaattttt tccaactatt tggagtgagg aagaaaagag aaattgggga
     3061 aaataatttt aaatttttca aatgaacact tctttagata tcctacaaat agaaaattac
     3121 actaaagaag atttattaaa atttattaat aatttaattc atcacgattt tgaaaaactt
     3181 gtttttcttc tatacagaat tgatgttgca gaagaaaaaa taaaaaactt attgagcaat
     3241 aatcatacaa ctaatgtgga agaattaatt gctaatgcaa taattgaaag agtgatggaa
     3301 aaaaagattt ctaaagaaaa atataaacaa caaaaccata tctcagaaga agagaagtgg
     3361 taagctaata tttactacaa ttatttatta agtttaagtg tattaatata ctttcgcaac
     3421 ataaacattt ttaccatgaa acttgtttct tatttaaaag atggacacga ccaattagca
     3481 tttttagtgg ataatttttt gtttgattgt gatatggtgc accccgaatt accgggcaat
     3541 atgaatatgt ttttacatta ttgggatgat atgtacccta ttgcacaaaa agtagatgca
     3601 gcaataaaag caggcagtat tagtaaagat agaggtattg atataagcac tatacaaata
     3661 attgctccaa taccatttcc aacaagttgt agagatggat atgcttttag acaacacgtt
     3721 gcagcagcca gaagaaatag aaaagttgat atgattgcag agtttgatca ataccctatt
     3781 ttttatttta caaatcattt aagcatacaa ggcgaaggaa atatttattg tatgcctgat
     3841 cattttgaga aattagattt tgaattagaa gcagcaattg ttatttgccg ccctggcaga
     3901 aatatacctg cagaagaagc agataaatat attggtggtt taatgattat gaacgacatg
     3961 agtgcaagaa ggttgcaaat ggaagaaatg ttgttaaact tagggcccgc caaaggaaaa
     4021 gatttttgta ccgtaattgg tccatgctta gttactttag atgagttgga agcatttgaa
     4081 attcctgcaa aagaaaacca tgttggtaaa tcatggaatt taaaaatgaa atgttgggta
     4141 aatggcattc aagtaagtga aggaaatgtt ggtgatatgg attggacttt tgcagaaatt
     4201 attgaacgtt gtagttatgg tgtaaatctt cacccaggag atgtaattgg tagtggaaca
     4261 gttggcacag gctgtttttt agaacttaat ggcacaggta aactaaacaa tccaaattac
     4321 caggagcaat ggttgcaaga aaatgatgta gtagaaatgg aaattgaaca aataggctta
     4381 ttaaaaaata caattgtaaa agaagaaagc tcattttcta ttttagccaa aaagaaatta
     4441 ccttaattta gtacaaacta catagctaac ttattgctaa tattgcaata agcatgagta
     4501 cacctttgca aatattacag caatattggc atcatcaaac atttagaggc gaacaagaaa
     4561 aaattattga agcagtttta aataaaaacg atgttgttgc tttattgcca actggtggtg
     4621 gcaaatctgt ttgctttcaa gtaccagcaa tgctaatgga tggtttgtgc ttagtaattt
     4681 ctcctcttat tgcattaatg aaagatcagg ttgaaaactt aaccaaaaaa aatatacctg
     4741 cagctgccat ttatagtggg atgagttttt ttgaaattaa taaaatactt caaagtgcta
     4801 cagataacaa tctaaagttt ttatatattt cacctgaaag aataaataca aaattatttc
     4861 agcaatgtat tcatcaatta aatatttgtt tagttgcagt agatgaagca cactgtatta
     4921 gccaatgggg ctacgatttt aggccatcct atttaaaaat tgcaaaaatt aaagagcaat
     4981 taaatagtgt gccatttatt gctttaactg caactgcaac tactgttgta aaaaacgata
     5041 ttattgacaa attacaattg aaaaatgtaa tagtttttaa acagtctttc gaaaaatcaa
     5101 atatttcttt ttctgttttt aatgttgata gtaaaataaa taaagcagta gaaatattga
     5161 ataaagtaca aggaagtagc attatttatt gtaaaaacag aaatgaaaca aaagcaatag
     5221 cagaacaatt attaaaacaa aatatatctg ccaattttta tcatgctggt ttaacgcaag
     5281 aggaaaggag tgtaagacaa aataattgga taaaaaataa aagcagggtt attgtttgta
     5341 caaatgcttt tggaatgggt attgataaac cagatgttaa aacggttata cattttaata
     5401 ttccagattg tatagaaaat tattatcaag aggctggccg tgcaggcaga aatggtagta
     5461 aagcttatgc tgttttgttg tatcaactat ccgatgaaat tgaattagat actttagcag
     5521 aggtaaaatt tcctgcaatt gaaacaatta aagaagtgta tcagcatatt gctaattatc
     5581 ttcaaatacc aattggcatt ggcgaaggca attattacaa ttttgacata attgattttt
     5641 gcaacaattt taaattagat gttgtacaag taattaatat tttaaaatat ttagagcagg
     5701 aaggtttttt tgctttttca gaaaatattt ttatacctgc aaaagtggtt tttacatcaa
     5761 ataaacaaac tttaaatagt gttgaaaaca gataccctca tttagattta gtaatgaaaa
     5821 ccttgcttcg tacttatgaa ggaatttatg aaaataatgt ttcaattaat gaaaaattga
     5881 ttgcaaaaat tgcaagacta tcttacgata cagtattttc taatttaaat acattacata
     5941 cattgggcat aattaactat atgccacaaa aagaaacacc tcaattgtat tatatgttaa
     6001 atagggcacc tgcaaatgga ttatttatca attattccac ttatctacaa agaaaaaaat
     6061 tatataaaga aaaaattgaa gccataaagc aatatgtaca tttagcagaa acttgtagaa
     6121 gtaaatttat tgcaaactat tttggagata aaaatgtgag caattgtaat tgttgcgata
     6181 attgtttaaa ccttaaaaag aacaaattat ctctcaaaga atttgaccaa attgaaaaac
     6241 taatatatcg ggtattgtcc aataacgaat taccactaaa tactttgcta caacagttgt
     6301 ctaattttaa aaaagaaaaa gttttacaag taattacatt tttagaaaca gaaaaattag
     6361 tatccaccag tagtacaggt ataattactt taaataaatt ataattttaa aatcattttt
     6421 aaccatctta attttccttt aaatggagga tattttatgt tagggtcaaa ccaggttgca
     6481 gattttaaaa caggtttggc atgtgtaaat gtgtaaaatg atttttttcc atgatagctg
     6541 ccaatgccac ttaggccaat gccaccaaat ggtaaatgat ggttagtgaa gtgatatgca
     6601 gtattgttaa tacacccccc accaaaagga attgcattta tccaattctc ttctttagtt
     6661 gtagaagaag taaaaatata aaatgctaaa ggggtagcat tctgctttat tatttctaca
     6721 gcttcagatt gcgtttcaaa atcaaatatg ggtagtattg ggccaaaaat ttcttcatgg
     6781 tttatagggg cattagtggg tatttcttgc attattgttg gttcaatgta aaggctttct
     6841 ttatcatact ttcctccata aataattttt ccttgttgta aaaaacttat taaagtattg
     6901 aacctttttt cgttaattat ttttccataa tctttgctta aggctgcatt attggtataa
     6961 aaactttgaa tagtagaaat caatatttct aaaaatttag cttttatttt tttatgaatc
     7021 aaaaggtaat ctggtgctat acaagtttgc ccagcgttta taaattttcc tattacaact
     7081 cttttagcag caatttttaa attggcatca tcttcaatta cacaagggct ttttccacca
     7141 agttctaaag taacaggtat taattttttg gctgccattt cataaattaa ttttcctaca
     7201 cttgtactac ctgtataaaa aacatggtta atacttcctt cttctataat agtaggtaca
     7261 atttgtgcac catcaccttg cactaaataa atataatctt ccgaaaaaat ttctttaact
     7321 attttctcca taattaaaga agtagcattt gcaaactcgc taggtttaac tactacacaa
     7381 ttgccagcag caattgctcc aactaaaggg ttaaaagcaa gttgaaatgg ataattccag
     7441 ggagcaataa ttaaaacaat gcctaatggt tcgtaataaa ttttactttt tccgggcaag
     7501 ttcattaaat tggtgctaac ttctttgggt tgcattaagc gttttagttg cttaataaaa
     7561 aacttaattt cattttgtac aatagctatt tcagaggcgt atgcttctgc ttcacattta
     7621 cctaaatctg tatgaagggc tattaatatt tccttctcat attttttaag tgcaaaagaa
     7681 atttttttta attgttctat tctaaataga tatttttttg tagcccctgt attaaaataa
     7741 tttcttaaat tttgtagttg ttctttcata tattaaatat aataacacaa gttaatcata
     7801 cattttaaaa taaagttcat atttgtaatt attaatggaa gatattttat ataccgacga
     7861 ttattttatg caacaagctt taaaggaagc aaataaggct tttaatgaag atgaagtgcc
     7921 tattggtgca gttgtagttg taaacaataa aataattagt aaagggtata accaaacaga
     7981 aaagctaaat gattgtacag cccatgcaga aatgattgca ctaacaagtg catttaattt
     8041 tttaggtgct aagtatattc cacaggcatc tttatatgta actgttgaac cttgtttaat
     8101 gtgctgtggt gctttgtatt ggagtaaact tgctaaaatt gtttatggtg caagtgatga
     8161 aaaaagtgga ggactaagat tttttaaaaa taatgatgga gaaattctta caagcaaatg
     8221 gccttttcat cctaaaacaa aattgataaa gggagtttta gaaaacgaat gtgaaatgct
     8281 tatgaaaact ttttttaaat taaagaggta ataataattt tttaaaaaaa tacatcttca
     8341 attaaaaaaa actcatggtc tttgttttta tttaactgta ccgacagtac atcaaattga
     8401 aattctttcc aacaggtatt taattctaaa taggctactg cagctttctt caatgcattc
     8461 attttaactt ggttaatgct ttcttccgga taaccaaaat tgctgttggt tcttgtttta
     8521 atttcaataa aatgtagctt attttctttg ctggcaataa tatctacttc acttctttta
     8581 tacctccaat ttctttccag tataaaataa ccttttaact caaaccattt tgctgataaa
     8641 tcttcgccta aattacctat attttttgtg tttaatttca ttatgtttga gcagaataaa
     8701 gttatacaaa acaaatgaac gataaaattt ttaaattaaa aggtgttgtt caacattatg
     8761 catggggagg gttttctttt attcctaatt tattaaaatt aaaaccaact aatcaacaac
     8821 cttatgcaga gtattggcta ggtgcacata cttcagcccc ttctgttgta gttaataaaa
     8881 atatttcttt acttgaatta attaatcaaa atccagaaaa aattgtaggg caagaagtag
     8941 ttaaacagta taatgggtta ccctacttat ttaaaatttt agatgttcat gaaatgttaa
     9001 gcatacaggt tcacccaaac aaagaagaag ctataaaagg ttttgaaaaa gaagagcagg
     9061 caggaatacc tattaatgca attaacagaa attataaaga caaaaatcat aagccagaag
     9121 ttatgattgc attaagtgag ttttggttgt tacatggctt taaagaaaaa tcagaaattg
     9181 aacaaactct aaccaaccat ttagagttaa atttattgct tccaaccttt aaaaaagaag
     9241 gaataaaagg gctatataaa attgcaatgg agctgcctca agagcaggta aatactattt
     9301 taaaaccaat agtaaaaaaa tctattttta ataaatctca aaatttaatt aataaacata
     9361 atacagagtg gtggattgca aaattttatg caaataatag tactgaaaat ttagacagag
     9421 gaatattctc tatactactt tttaatattg ttcaactgca aaaaggagaa gcaatatttc
     9481 aggcagctgg aataccacat gcatatttag aaggacaaaa tgtagagtta atggccaata
     9541 gcgataatgt attaagaggt ggattaactc ccaaacatat agatgtacct gagttattaa
     9601 aacatacttt atttcaatct attcaaccct ctaccataaa acctcaaatt aatggtaacg
     9661 aaaaaactta tttatgcccg gtaccagatt tttctattag catcatttca ttaaaagtag
     9721 gcgaaagata ttcttctcac actttttctt ttgaaatatt attagtaatt gaaggtgcca
     9781 taataattca ggggttagaa aacaacataa taaaacaagg agaagccatt ggaattgttg
     9841 ctgatacaaa ctacactttt actgctacgg gagatgcaat tatttttaaa gcatttgtac
     9901 ctcaacctta aaattttctt tattcaacaa atctcatttg aaacaaacgt tataacgcct
     9961 tatttttgta aaaatttttc actatcatga aaattaatcg ttccgtttta tttacagttg
    10021 caattataat tttaactgta gtagtaaagt ttatttgtgc cccaatatta agtttaagtg
    10081 gtatttcaac cgttattgca gtagcattgt ttgctggcat gagcattcat aaaaaacaaa
    10141 acacctttat gttgccatta atagcattaa ttgttagtga tattataata gaagtattgt
    10201 accagtttaa ttggtttcag ttttcgggtt tttataaagg gcaaatattg aattacggtt
    10261 tattattatt aagtgtacta gttggttggg caattaaagg taaaagtatt ctatccattg
    10321 gtttaggcat atttgctgct tctaccatat ttttcatctt atctaattta agtgtttggt
    10381 attttggaac catgtacaca aaagatttta gtggtttaat gctttgttat gaaagtgctt
    10441 taccatttta taaaaaccaa ttaatatcta ctgctatctt cttgcctata attatgtttg
    10501 cttataacta ttttatagaa aagaaatacc gtgtagccgt atcacattag taataaaata
    10561 ttcattcatt caatcctgta atttattaca ggattttttt attttaatat attgattatt
    10621 aatcaattaa taataaattt ggatgaatta aagcattatt tttatctatt ggacagataa
    10681 ttttttaaaa taaattgtat aaaattgatt ttttttcaaa aaaactttgc acagtaactc
    10741 taaagcccct acttttgcaa ccccaacgga aaattgggtt ttagtatgtc tcaaagaatt
    10801 agaattaaat tacaaagcta cgatcacaat ttagtagata aatctgctga aagaattgta
    10861 aaaacagttc gtagcacagg agccgtagta accggtccta ttcctttgcc tacacgtaaa
    10921 agaatattta cagtattacg ttcaccacac gttaacaaaa aaagccgtga gcagtttcaa
    10981 ttgtgcacac ataaacgctt gttagatatt tatacttcta cttcaagtac tgtagatgca
    11041 ttaagtaaat tagatttacc aagtggtgtt gaggtggaaa ttaaggcata acaaacggcg
    11101 tttttcgttt tacgctaaat aaacgaacag ttcttataaa acaaatattt atctctcaat
    11161 tcgtactacg aagaaaagag ctctgaaaaa taaaagtcct aaaaatttta ttatcaaaat
    11221 atttaggaaa aacatataaa caagcccttc gaggtttgtt tactatcggt acttcccctt
    11281 tcttaattta agaaaaacaa aggaaaaggt cggtagcaaa caaggattga attatgcttt
    11341 tcatcagcat aatgtccttt ataacctcag cgggcacaaa ccgcaaaacg atgaaaggaa
    11401 ttattggaaa aaaaattggg atgactagca tcttctctgc cgatggtaag caaacagctt
    11461 gtaccataat agaagttgct cctaatgttg ttacacaagt taaaaccatc gatacagatg
    11521 gttacaatgc acttcagctt gcaattggtg ataaaaaaga aaaacatgct acaaaagcag
    11581 aaattaatca ctttgctaaa gctaacactc cagctaaaca atttacaaaa gaatttcgtg
    11641 attattcttt aactaaaaac ctaggcgaaa caattactat agacattttt gaagaaggtg
    11701 ataaggttga agtagttggt acaactaaag gtaaaggctt tcaaggtgtt gtaaaacgtc
    11761 atggatttag tggtgtgggt gaaggaagcc acggtcaaca tgatcgtcaa agagcaccag
    11821 gttcaatagg cggttcttct tatccttcta gagtttttaa aggaatgaga atggcaggcc
    11881 gtatgggagg cgatagagta aaaactaaag gtttagtagt attaaaagta tttccagaaa
    11941 aaaattatat tctaataagt ggttctgttc caggacacaa taactcaata gttttaattc
    12001 agaaataatc actctttaat tattcttaca atgcaaatag atgttttaaa tataaaaggt
    12061 caaaaaacag gtagagttgt tgaattgcca gaagatgtat ttggtgcaga gcctaatgac
    12121 cacgttattt atttagcagt taaacaatac ttatctgcta accgccaagg tacacacaaa
    12181 gtaaaaacac gtgccgaagt acatggtgcc agtagaaaat tacataaaca aaaaggaact
    12241 ggtggtagcc gtaaaggaaa tattagaaac cctttatata aaggtggggg tactattttt
    12301 ggacctaaac ctcatggcta ctcaattaaa ttaaatcgta aagtaaaaga tttagcaaaa
    12361 atttctgcac ttagctataa ggcaaaagaa aatgcaattt tggtggtaga agatatcaat
    12421 ttagattctc ctaaaacaaa acaaattgca gaagctatga atgctttaaa tgttgctgac
    12481 aaaaaatcaa tattagtatt aggagagtat aatgataacg tttatttaag tactcgtaat
    12541 atacctaatg ttgctagcac attattagca gatatgaata catacgatat tgtaaatgcc
    12601 gatgtaatag tattaacaga gaatgctgca aaaattttta gtgaaaacga agtagctaca
    12661 gaagcttaaa ataatataat aatcgtttca atttaaatac aatgaaactt acagaagtat
    12721 tagtaaaacc aattttaacc gaaaaggcaa atgcacagca agaaaaattg cgtagatatg
    12781 cttttagagt taataaaaaa gcaaataagc ttgaaattaa aaaagctatt gaacaatttt
    12841 atggcgtaac tgtaacaaat gttaatacaa caattgctgc aggaaaaaat aaaacacgtt
    12901 ataccaaagc cggctttatt gaagggaaaa aagctgctta caaaaaagca tttgtaacag
    12961 tagccgaagg ggaaacaata gatttatacg caaacattta attatagcta acgaagctga
    13021 aaaattcgtt tataaaaaag taaaaacatg gcattaaaaa agtttaaacc agttacagca
    13081 ggcacacgct ggaaaattgg taacgcttat gcagaaataa caactaataa accagaaaga
    13141 agtttattag aacctgttaa aaaaacagga ggacgaaatg ctcaaggtcg cagaacaatg
    13201 agatatattg gtggtggtca taaaaagcat tatcgtatta tagattttaa acgtaataag
    13261 caaaatattg cagctactgt tgtatcaatt gagtacgatc caaaccgcac tgcatttata
    13321 gcattattaa actataccga tggagagaaa agatatatta ttgctccaca aggtttacaa
    13381 gttggtgcaa ccgtaattag cggtaatgaa gcacctccag aaattggcaa tgcattacaa
    13441 atgaaaaata tgcctttagg tacaatgatt cataatatag aaatgcaacc aggccaaggt
    13501 ggtaaattag taagaagtgc aggagcctca gctcaactta ccaacaaaga agaaaaatat
    13561 gcagttctta aaatgccaag tggtgaatta agaaaagtgt taatcaactg ctttgcaacg
    13621 gtaggtgttg tttcaaatag cgatcatagc ttagaaactg caggtaaagc tggtgcaaat
    13681 cgttggaagg gaataagacc aagagtaaga ggtgttgcaa tgaacccagt tgatcaccca
    13741 atgggtggtg gcgaaggtag agcatcaggt ggtcaccctc gtagcagaac tggtaaatat
    13801 gcaaaaggag aaaaaacaag aacaaaagga aaaggcagcg ataaattaat tattcaacgc
    13861 cgagatggta aaaaattaac aaaataattt aaaagttaaa atattctctt taataagagg
    13921 aaaataaaca aaataacaat atggctcgtt caattaaaaa
//
LOCUS       DAOQPK010000035        13489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_2361_length_13489_cov_4.769391, whole genome shotgun sequence.
ACCESSION   DAOQPK010000035 DAOQPK010000000
VERSION     DAOQPK010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13489
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2361_length_13489_cov_4.769391"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..629
                     /gene="rdgB"
                     /locus_tag="PLU36_02965"
     CDS             27..629
                     /gene="rdgB"
                     /locus_tag="PLU36_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HMZ45744.1"
                     /translation="MTEKFTLIFATNNANKVQEIKSDLGNNFEIISLKEAGIDIEIPE
                     PYDTLKENATEKSSVIFRLTNKNCFSEDTGLEVEALHGEPGVKSARYANEYPKFESNI
                     EKLLYNLKDIKNRNAQFRTVISLIINKQEYFFEGICKGKIIDTPKGNEGFGYDPIFVP
                     DGSNKTFAEMPIEEKNKFSHRKKAMQQLILFLSQNKTNFS"
     gene            733..1017
                     /locus_tag="PLU36_02970"
     CDS             733..1017
                     /locus_tag="PLU36_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45745.1"
                     /translation="MASSAIAFNNDLLKENWRVKPSSSTITKEAKVSYLMVEQVEPPK
                     YMWDKICKILDAEKEAENKTNYQKSSNKTIKALLIAGGLITIASILLFLL"
     gene            1098..1532
                     /locus_tag="PLU36_02975"
     CDS             1098..1532
                     /locus_tag="PLU36_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HMZ45746.1"
                     /translation="MQRVKVELPINFNFSTIIPIRITDMNYGGHVGNDSFLSLIHEAR
                     MQYLQHFGYTEINFENTSLIMADAAIEFKHEIIYTNTIKISVQAAAFTKIGFDLYYLL
                     EIINGEELIIAGKAKTGMLCFDYTNKKITTVPTKVISKLTTQ"
     gene            1543..2571
                     /gene="thiL"
                     /locus_tag="PLU36_02980"
     CDS             1543..2571
                     /gene="thiL"
                     /locus_tag="PLU36_02980"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HMZ45747.1"
                     /translation="MQQRTEIANLGEFGLIEHLTKNFEIKNASTILSVGDDAAVIDHF
                     GKQTVITTDMLVEGIHFDLMYTPLKHLGYKSVIVNLSDVYAMNAIPTQITISIAFSNR
                     FSVEALSEFYEGVYAACEKYAVDLVGGDTTSSQKGFIISVTAIGEVAPDKYVQRSTAQ
                     QGDLICVSGNTGGAFLGLTLMQREKKIYLENPAIQPDLEGEDYIVGRLLKPEARKDII
                     EFFAANNIVPTAMMDVSDGISSEVLHLCKQSNVGCKIFEDKLPIDEKTRAAAFKFGLD
                     PTVCALNGGEDYELIFTLKQEDYDKITLNEEIAVIGYMTALEEGNKLQTKGGNIFDIT
                     AQGWNAFT"
     gene            2623..4101
                     /locus_tag="PLU36_02985"
     CDS             2623..4101
                     /locus_tag="PLU36_02985"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HMZ45748.1"
                     /translation="MDSIVILFSLLTSCVSYKIPNYTYNKKLPVALLKEDLTILKKTL
                     EANHPSLYWYNTKDSIDFNFENALSTMKDSLNEVQFKKKVAEIISTIHCGHTSVNYSK
                     NFTKLYKHHLYPMFPLYLKVWADSMVVLGNTIETDSILKRGVIITTINGVSNRLILDS
                     IFKSISTDGYSVNYKNQVVSNNFPAWYKQTWGLDTSYTISYLDSLGNKATTTIKNYSP
                     IKDTSTIKKEITKIKKPNRKQLKNARRLAKRYLQIDTSLQTAYIRINTFSEGRLRKFF
                     RKTMKTISKDEIKNVVIDLRQNGGGDVGTTTKLLKYFANSPFKHADTVEAINRTFPHK
                     KYLKNWWLYWIPMNFFATKERDKKIHYRRFEHHYFKPKSRYHFNGQVYLIQGGMTFSA
                     ATMFIATLKGQENVIVVGEETGGGFYGNSAVHLPTIILPNSKLRISLPMYRMVMNKNR
                     PKGRGIIPDIYIPPSSIAIKEGYDTKIVTIRKLIEEKNMQPK"
     gene            complement(4430..5593)
                     /locus_tag="PLU36_02990"
     CDS             complement(4430..5593)
                     /locus_tag="PLU36_02990"
                     /inference="COORDINATES: protein motif:HMM:NF022653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2989 domain-containing protein"
                     /protein_id="HMZ45749.1"
                     /translation="MNNVIEKYCNFFIAHFGLNIAKYRQRNYYSIFFKTNIQYITMKH
                     LFILIVVCFTSATSFANSNSINNNSDSANYYYAKAKESRSQRKVFDAEKFFQKALEFD
                     ANNDEIRIEFAEYYIEMRKYALATIQYRTILKDNPTHKIALPKYIGLCFLQRKWVEVI
                     KNGEIALTNNIKVENINFMMGKSYYEEEDYGKARKYLTTQFKQNPKHKETVILLGRVY
                     IEMSMYNDAIAMYKQAIESNPDDYEILYEIGLLYSAQNNDREAVKYFEMAADKGIKQD
                     LAFLQNLGMAYLSFNIPKGVEVLNKVLEKKPGDVEILTQIAQAYYKAQDYGTAYELYF
                     KIFENDNSNAKALYMAGVSLIRKGDKNRGTKLCDKAIAMEPKLGELRSQKSVL"
     gene            complement(5724..7757)
                     /gene="glgB"
                     /locus_tag="PLU36_02995"
     CDS             complement(5724..7757)
                     /gene="glgB"
                     /locus_tag="PLU36_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HMZ45750.1"
                     /translation="MDKRQHNNLYGSSKKVNLQNKTAVHKNAPKSKLKYEDKYFIDTT
                     KSVWNYSLFTATDIKNFKNGTHYSLYKLLGNKQIEVNNLKGTYFAVWAPNATYVSVIG
                     NFNNWKVKSHPLNVRGDSSGIWEGFIPSILRGEAYKYHIVGYKGIKLDKGDPFAHFWE
                     KRPATASICWDTYYEWKDVKWMKERKKNNALNAPWSVYEVHLASWMRPEKNNENLYNS
                     YQLITQKLVPYVQEIGFTHVEIMPVMEHPFDGSWGYQGTGYFAPTSRFGSPQDFAAMI
                     DAFHVAGIGVILDWVPSHFPFDAHGLYLFDGAHTFEYGDMRKGFHPDWNSYIFNYKRP
                     EVKSFLISSARFWCDRFHIDGLRVDAVSSMLRLDYSREEGEWEPNEFGGNGNLDAIDF
                     IKKLNETLYLDFPDIQTIAEEATDWPLISKPIYDGGLGFGMKWMMGWMHDTLNYFKRE
                     PKDRLQHKNDFTFSMMYFYNENFMLPLSHDEVVHGKSPMIYKMPGNTKQKFANLRLLY
                     TYMFTHPGAKLLFMGNEFAQTTEWNFKSELDWELTDLVSHGGMLYCIKKLNELYKNNP
                     ALYELQFSKEGFEWVNLNNTSQAIFCYLRKGKSTKDYMLIILNMSNQVQHNWKIEVGL
                     KNKWKEIFNSDSKEFWGSGLVYNNEIQCVKTNKLNKKCTLSLSLPALSAIILQ"
     gene            7904..10675
                     /locus_tag="PLU36_03000"
     CDS             7904..10675
                     /locus_tag="PLU36_03000"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMZ45751.1"
                     /translation="MKYFFIVFIILICFQKSNAQNASIKGVITDTLNKHNLTNAVISL
                     LHSKDSILYTFTRSDINGNFNLSKINKGKYIVLVTYPTYADYFDTIHILNDNNINLET
                     VKLTPKAHLLADVTVVSKIAAVRMKGDTIVYKADSFKVKQGATVEDLIKLFPGIQVDK
                     NGNITAHGTRVEKVMVDGEEFFGDDPTIATKNLSADMVNEVQVFDKKSDQATFTGVDD
                     GQTSRTINLKLKENAKKGWFGKLEASIAIKDVWNNSLMANSFKGKRKLSFYGIASSNG
                     KTGLNWDESSKYGSQSNTSFSMSDDGSTMYFEGDDFDGMEYFGQGLPKSWSGGINYGN
                     KFNNNEQQVNGSYLYSKVINEGTGYSKSKSLLPDTVFFNNENNKTTGVKDKHGINGTV
                     DWQMDSLLSLKVTVSASKGNSYGNSNYYNEALNEQNQYVNNSIRNKINQGKNKNINYN
                     VLVRKKFKKIGRTLSLSLEQKFNTQNNDGYLYSENNFYNANGSLNFSDTTDQKKLSDN
                     NTNNLKSKIAFTEQLLKSLVVEFSYTYQLNNYTNKVLSYDKNNSGKYEDFNTKFSNNF
                     KFTNATSLIGTMFRYQKNKLNVSFGGNVSLANFKQKDLIKDTTYHYQFNNIYPTAILT
                     YKFNSNKRITINYNGNTKQPSINQLQPVADNNNPLFIQKGNPNLVQEFNHAFAVNYNN
                     YQVLKNRGYGFRIRYNTTYNAIRSSIYTDSLGRSISQYVNLNGNNYFTTNINYNTKVK
                     KLDIYVNASYSFNIGNSISIVNNQTNKNVNKSHQMYVGISKEKENKFNLWLYNSIAYN
                     ISSSSIRSEINTQYWSASLGADLSVYLPWKLELNNNIEAELRQKTELFSGNNNITIWN
                     IYISRKIFKGDKSQLRFTAFDILNQRRNYERDISSNTITETGYQQLSQYFLFSFIWNF
                     NKGGAAITK"
     gene            10683..11450
                     /locus_tag="PLU36_03005"
     CDS             10683..11450
                     /locus_tag="PLU36_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HMZ45752.1"
                     /translation="MKSILLSFIFYFLFINLPAQQIFINTGKIEFEKQVNLHKVLELS
                     WGDEENDNVWIMNMKKELPKIKSTFYNLYFTQNKTIYKAGKEAPQTAQKIPEWLADND
                     ALENITYTDLATHKTIAKKKVFETTFLLEDSITKINWRITNDTRTIAGIECRKAVGKI
                     FDSVYVIAFYTDLITCTGGPLSFTGLPGMILGVAIPRIHTTLFATKVELANIDETIFA
                     IPKSGKKTSYVNLLKQLKSSMNDWGKEGNLIIWKTLL"
     gene            complement(11457..12857)
                     /locus_tag="PLU36_03010"
     CDS             complement(11457..12857)
                     /locus_tag="PLU36_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HMZ45753.1"
                     /translation="MVLTICYLIGSITFIIGLKMLSHPASARKGNLVAAAGMAIAIVG
                     TIFLYEEEGKKLGNYFWIFGGLAIGTVVGTLIAKKVKMTAMPEMVSLFNGMGGACAAL
                     ISIIEFQHIAHHLNNNVTANYGLLISIFLGAIIGSVSFAGSMIAWGKLNGTIKDFSYN
                     GQQFINVGLMAVILGIAGYLVFSLSLTNAYWFYIIFVVSLLYGVLFVMPIGGADMPVV
                     ISLLNSFTGVAAACGGFLYDNKVMLTGGILVGAAGTLLTILMCKAMNRSLKNVLLGSF
                     GGNKAGAAGSKEQGSFKEIQLSDAATILAFANKVMIVPGYGLAVAQAQHTCHELEKLL
                     EDKGVEVKYAIHPVAGRMPGHMNVLLAEADVSYDKLLEMEDANEEFRSTDVVLVLGAN
                     DVVNPAAKKDPASPIYGMPILEVEQAKNVIVNKRSMKPGYAGIENDLFFQPKTSMLFG
                     DAKAALQKLITEIKSV"
     gene            complement(12910..13218)
                     /locus_tag="PLU36_03015"
     CDS             complement(12910..13218)
                     /locus_tag="PLU36_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HMZ45754.1"
                     /translation="MENILNFIKEHEQIIYIVVLMIFLGIEVIGKVPSVLHTPLMSGA
                     NAIHGVVIIGAIIVMGKAEADNYLALVLGFLAVILGTLNVVGGFVVTDRMLEMFKKKK
                     "
     gene            complement(13254..>13489)
                     /locus_tag="PLU36_03020"
     CDS             complement(13254..>13489)
                     /locus_tag="PLU36_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HMZ45755.1"
                     /translation="KNNENFTTENGVLIVGNSNLPCGMPFDASKMYGKNILNFLQLII
                     TNEGALNLNFEDDLVKGTCITHNGSIVHNRLID"
BASE COUNT         4974 a         2089 c         1895 g         4531 t
ORIGIN      
        1 taaggcaaat ttttaaatta taatgaatga cagaaaaatt tactttaatt tttgcaacca
       61 ataatgcaaa caaggttcaa gaaataaaat ctgatttagg aaacaatttt gagataatta
      121 gtttaaaaga agcaggaata gatattgaaa taccagagcc ttatgatacc ttaaaagaaa
      181 atgcaacaga aaaatcgtct gttattttta ggctaacaaa taaaaattgt tttagcgaag
      241 atactggatt agaagtggaa gctttacatg gagaaccagg tgtaaaaagt gccagatatg
      301 ccaatgaata tccaaaattt gaatcgaata ttgaaaagct tttatacaat ttaaaagaca
      361 taaaaaatag aaatgctcaa tttagaacag taatttcttt aattataaat aaacaagaat
      421 atttttttga aggaatatgt aaaggtaaaa ttatagatac tccaaaaggc aatgagggct
      481 ttggttacga ccccattttt gtgcctgatg gcagcaataa aacctttgct gaaatgccaa
      541 ttgaagaaaa aaacaaattt agtcacagaa aaaaagccat gcagcaatta atactttttc
      601 tttcccaaaa taagactaat ttttcctaat tcaagatttt ttttgcattc atgcaactat
      661 ttaatctcta tttctatctt gtttttatac agtgttttta agtacctaat tattctatca
      721 aaagcattta ttatggcatc atctgctatt gcattcaata atgatttgtt aaaagaaaac
      781 tggagagtga aaccttcctc atctaccata actaaagaag ctaaggtatc ttacctaatg
      841 gtggaacaag ttgaaccacc aaaatatatg tgggataaaa tttgtaaaat attagatgca
      901 gaaaaagaag cagaaaacaa aaccaattat caaaaatcat ctaataaaac cattaaagct
      961 ttactgattg caggcggttt aattacaatt gcttccatat tacttttttt actttaattg
     1021 caccagctac attccaattt tttcaatttt atacttaaaa aacttctaat tcaatattct
     1081 tacactattt tgcaacaatg caaagagtaa aagttgaact tcctattaat tttaattttt
     1141 ctactattat tcctattaga attactgata tgaattatgg tggacatgta ggcaatgata
     1201 gctttttatc gttaatacac gaagccagaa tgcagtattt gcagcatttt ggttatacgg
     1261 aaattaattt tgaaaatact agtttaatta tggccgatgc agctatagaa tttaaacacg
     1321 aaattattta tacaaataca attaaaattt cggtacaagc tgctgccttc accaaaattg
     1381 gatttgattt atattatctt ttagaaatta tcaatggaga agaattaata attgcaggca
     1441 aagccaaaac aggtatgtta tgctttgact atactaataa aaaaataaca actgtaccca
     1501 caaaagttat tagtaaatta acaacacaat aaacaaatta caatgcaaca aagaacggag
     1561 attgctaact taggagagtt tggattgata gaacatctca ctaaaaattt tgaaataaaa
     1621 aatgcatcaa ctatactaag tgttggagat gatgctgctg taattgacca ttttggaaaa
     1681 caaactgtta ttacaacaga tatgttggta gagggtattc attttgattt aatgtacacc
     1741 cctttaaaac atttaggtta taaaagtgta atagtaaact taagcgatgt gtatgcaatg
     1801 aatgctatac caacacaaat tactattagt attgctttta gcaacaggtt tagtgtagaa
     1861 gccttaagcg aattttatga aggtgtttat gcagcttgcg aaaaatatgc tgtagattta
     1921 gttggaggtg atacaacttc ctctcaaaaa ggatttataa tatctgttac tgcaattggc
     1981 gaagttgcac cagataaata tgtacaaaga agtacagcac aacaaggaga cttaatttgt
     2041 gtatctggta acacaggtgg tgctttttta ggtttaacac ttatgcaaag agaaaaaaaa
     2101 atatatcttg aaaaccctgc catacaacca gacttagaag gagaagatta tattgtaggc
     2161 aggttattaa agcctgaagc cagaaaggat attatagaat tttttgctgc aaataacatt
     2221 gtaccaactg caatgatgga tgtaagtgat ggcattagta gcgaggtttt acacctttgc
     2281 aaacaaagta atgtaggttg taaaatattt gaagataaat tacccattga tgaaaaaaca
     2341 agagctgctg cttttaaatt tggcttagac cccactgttt gtgcattaaa tggcggcgaa
     2401 gattatgaat tgatttttac cctaaaacaa gaagattatg ataaaattac tttaaatgaa
     2461 gaaatagctg taataggcta tatgactgca ttagaagagg gtaacaaatt acaaaccaaa
     2521 ggaggtaata tttttgatat tactgcacaa ggttggaacg catttacata aagtttataa
     2581 aaaattgttc tttcattgaa gaagaaattg atacatattt atttggatag tatagttata
     2641 ttattttcct tgcttacttc atgcgtatct tataaaatac caaattatac ctataacaaa
     2701 aagctacctg tagcattatt aaaagaagat ttaaccattt taaaaaaaac tttagaagcc
     2761 aatcacccat ctttatattg gtacaatacc aaagacagta tagattttaa ttttgagaat
     2821 gcactttcta caatgaagga ttctttaaat gaagtacagt ttaaaaaaaa ggttgctgaa
     2881 attataagta caatacattg tggccatacc agtgttaact attctaaaaa ttttaccaag
     2941 ctttacaaac atcacctcta ccccatgttt cctttatact taaaagtgtg ggcagatagt
     3001 atggttgttt taggtaatac aattgaaaca gattccatac ttaaaagagg cgttattatt
     3061 accactatta atggagttag caatcgactt attttagatt caatttttaa aagtattagt
     3121 acagatggtt atagtgttaa ttacaaaaat caggtagtaa gcaataactt tcctgcatgg
     3181 tataaacaaa cctggggttt agatacaagt tatacaatta gctatctgga tagtttagga
     3241 aataaggcaa ctactaccat aaaaaattat tcccctataa aagatacttc tacaataaaa
     3301 aaagaaatta caaaaattaa aaagccaaat agaaaacaac ttaaaaatgc aaggcgttta
     3361 gcaaaaagat atttacaaat tgatactagt ttacaaactg catacattag aataaatact
     3421 tttagtgaag gaagattgag aaaatttttt agaaaaacga tgaagactat aagtaaagat
     3481 gaaattaaaa atgtagtgat tgatttaaga caaaatggtg gtggagatgt aggtacaacc
     3541 acaaaacttt taaagtattt tgcaaactct ccctttaaac atgctgatac tgtggaagca
     3601 attaacagaa cttttccgca taaaaagtat ttaaaaaatt ggtggcttta ttggatacca
     3661 atgaactttt ttgcaaccaa agagcgagat aaaaaaattc attatagaag gtttgaacat
     3721 cattatttta agcctaaatc aagatatcat tttaacggac aagtgtattt aatacaaggt
     3781 ggaatgactt tttcggctgc aactatgttt attgcaactt taaaagggca agaaaatgta
     3841 attgttgttg gagaagaaac aggaggaggt ttttatggta actctgcagt gcatttacca
     3901 accattattt tgcctaatag caagcttaga atttctttgc caatgtatag aatggtaatg
     3961 aataaaaata gaccaaaagg aagaggtata ataccagata tatacatacc ccctagttca
     4021 attgcaatta aagaaggtta tgatacaaaa attgttacca ttagaaaact aattgaggaa
     4081 aaaaatatgc agcctaaata atttttaact cattagcatc gtttggtttc acatttttat
     4141 cagtcagacg acaatcgtca gactgataat attcaaactt agtttacaaa taagtttttt
     4201 aattcattag catcatttgg ttttcatttt tcctgcaaga atgagccttt tatcagtcag
     4261 acgacaatcg tcagactgat aatatcaaaa cttagtttac aaataaattt cttaattcat
     4321 tagcatagtt tgttttcatt tttcctgcaa gaatgagact tttatcagtc aaacgacaat
     4381 cgtcagactg ataatactaa ttgaggaaaa aaatacgaag cctgaataat tatagaacag
     4441 atttttgaga tcttaactca cctaatttag gttccattgc aattgctttg tcacatagct
     4501 tagtgcctct atttttatct ccttttctta ttaaagaaac accagccata tataaagctt
     4561 ttgcattgct attatcattt tcaaatattt taaagtacaa ttcgtaggca gtgccataat
     4621 cttgagcttt gtagtaagct tgtgctattt gagtaagaat ttccacatcg cctggttttt
     4681 tctccaacac tttattcaac acttctacac cttttggaat attaaatgac aggtatgcca
     4741 tgcctaagtt ttgtaaaaag gccaagtctt gttttattcc tttgtcggct gccatttcaa
     4801 aatatttcac agcttctcta tcgttatttt gtgcactgta taataagcct atttcatata
     4861 aaatttcata atcatcaggg ttgctttcaa ttgcttgttt atacattgct attgcatcgt
     4921 tatacatgct catttctata taaacccttc ctaataaaat aacagtttct ttatgtttag
     4981 ggttttgttt aaattgagta gttaaatatt ttcttgcctt tccataatct tcttcttcgt
     5041 aataactttt acccatcata aagtttatat tctctacttt aatattattg gtcaaagcaa
     5101 tctctccatt tttaattact tctacccatt ttctttgtaa aaagcaaagc ccaatatatt
     5161 ttggaagtgc aattttatgt gtaggattat cttttagaat tgttctatat tgaattgtag
     5221 ctaatgcata cttacgcatt tcaatataat attctgcaaa ttcaattctt atttcatcat
     5281 tattagcatc aaactctaat gctttttgaa aaaacttttc tgcatcaaaa acttttcttt
     5341 gagatcttga ttcttttgct tttgcataat aatagttggc actgtcgcta ttattattaa
     5401 ttgaatttga atttgcaaag gatgttgcag aggtaaaaca aaccacaatt aaaatgaata
     5461 aatgtttcat agtaatgtac tgaatattgg ttttaaaaaa aatactataa taatttcttt
     5521 gcctgtattt tgcaatattt aagccaaaat gagcaataaa aaaattacaa tatttttcta
     5581 taacgttgtt catgataaat ttattttaca acaaccaatt aaaaattaaa acaacaatat
     5641 aatagattga caaacataat attttacaca aacgggggaa tgatagtatg aagttataat
     5701 ttttttatat aaacaatagg atattattgt aaaattatag ccgaaagtgc tggtaagctt
     5761 aaagaaagag tgcatttttt atttaactta tttgttttaa cgcattgaat ttcattatta
     5821 taaaccaaac cagaacccca aaattcctta ctatcgctat taaaaatctc tttccattta
     5881 ttttttaaac caacttctat tttccaatta tgctgcactt gattgctcat gtttaaaatt
     5941 attaacatat aatctttagt actcttccct tttcttaaat aacaaaagat tgcttgagaa
     6001 gtattattca agttaaccca ttcaaaccct tctttactaa attgtaattc atataatgca
     6061 gggttgtttt tgtatagttc atttaatttt tttatgcaat acaacattcc accatggcta
     6121 accaaatctg taagttccca atctaattca ctcttaaaat tccattcggt tgtttgagca
     6181 aattcgttac ccataaacag taatttggca ccagggtgtg taaacatata tgtataaagc
     6241 aaccttagat ttgcaaattt ttgtttagtg tttcctggca ttttataaat catggggctt
     6301 ttgccatgta ccacttcatc atgacttaag ggtaacataa aattttcgtt ataaaaatac
     6361 atcatgctaa aagtaaaatc gtttttatgc tgtaatctat cttttggttc tcttttaaaa
     6421 tagtttaaag tgtcatgcat ccatcccatc atccatttca ttccaaagcc taaaccacca
     6481 tcataaattg gtttactaat taaaggccaa tctgtagctt cttctgcaat agtttgtata
     6541 tctggaaaat ctaggtataa agtttcattt aattttttaa taaaatcaat agcatctaaa
     6601 tttccattac ctccaaactc atttggctcc cactctcctt cttctctact ataatctaat
     6661 cgtaacattg atgaaacagc atcaaccctt aacccatcaa tatgaaatct atcgcaccaa
     6721 aatcttgcac tacttattaa aaaacttttt acttcgggtc gtttataatt aaaaatataa
     6781 ctgttccaat ccggatgaaa cccttttctc atatcgccat attcaaaagt gtgagcacca
     6841 tcaaataaat ataaaccatg tgcatcaaaa ggaaaatgag agggtaccca atctaaaata
     6901 acaccaatac cagctacatg aaatgcatct atcatagctg caaaatcttg tggactacca
     6961 aacctacttg ttggagcgaa gtaacctgta ccttggtagc cccaactacc atcaaaagga
     7021 tgttccatta caggcataat ttcaacatgc gtaaacccaa tttcttgtac ataaggtact
     7081 aacttttggg ttattaattg atagctgttg tataggtttt cattattttt ttcaggcctc
     7141 atccaactgg ctaaatgtac ttcatataca ctccatggtg catttaaagc attgttcttt
     7201 tttctttctt tcatccactt tacatccttc cattcataat aagtatccca gcaaatactt
     7261 gctgtagcag gccttttctc ccaaaagtgt gcaaatggat ctcctttgtc tagctttatt
     7321 cccttataac caacaatatg gtatttatat gcttcgcctc ttaatatact tggaataaaa
     7381 ccttcccaaa taccactgct atctcctcta acatttaatg gatgagattt tactttccag
     7441 ttgttaaagt tgccaataac cgaaacataa gtagcattag gagcccaaac agcaaaataa
     7501 gttcccttta aattgtttac ttctatttgt ttattaccta ataatttgta taaactgtag
     7561 tgagtaccat ttttaaaatt ctttatatct gttgcggtaa ataatgaata attccaaaca
     7621 gatttagtag tatctataaa gtatttgtct tcgtatttta atttactttt gggtgcattt
     7681 ttatgaacgg cggttttgtt ttgtagatta acttttttac tagaaccata taaattatta
     7741 tgttgccttt tatccattat aaatattaat aaatttaata acgcaatatt tttaactcag
     7801 taaattaaac cttcttttaa tacacattat ttgagttagc ccaagaagat gatttttaaa
     7861 tcttaaataa aactacactt taataagtaa ttgaaatagt aatatgaaat atttttttat
     7921 tgtttttatc atattaatat gctttcaaaa atctaatgct caaaatgcaa gtataaaggg
     7981 tgttattaca gacactttaa acaaacataa tttaactaat gctgtaatta gtttattaca
     8041 ttcaaaagat tctattttat atacttttac aagaagcgat ataaatggaa attttaacct
     8101 ctctaaaatt aataaaggga aatatattgt tttagttacc tatcctactt atgcagatta
     8161 ttttgacact atccatattt tgaatgataa caacatcaac ttagaaacag ttaaattaac
     8221 acctaaagct catttattgg ctgatgttac tgttgtaagt aaaatagctg ctgtgagaat
     8281 gaaaggcgat accatagtgt ataaagcaga tagctttaag gttaaacaag gagctactgt
     8341 agaagatctt atcaaactat ttcctggtat tcaggtagat aaaaatggaa acataacagc
     8401 acatggcaca agagtagaaa aagtaatggt agatggtgag gaattttttg gagatgaccc
     8461 aaccattgca acaaaaaatt taagtgccga tatggtaaat gaagtacaag tatttgataa
     8521 aaaatcggac caagcaactt ttacaggtgt ggatgatggc cagactagcc gtaccataaa
     8581 tttaaagtta aaagaaaatg caaagaaagg atggtttgga aaattagaag caagtattgc
     8641 tattaaagat gtttggaata attctttaat ggctaatagt tttaaaggta aaagaaaatt
     8701 atctttttat ggtattgcaa gtagtaatgg caaaacaggt ttaaattggg atgagtctag
     8761 taaatatggc tctcaatcta acacttcttt ttctatgagt gatgatggat ctacaatgta
     8821 ttttgaagga gatgattttg atggtatgga atattttggg caaggtttac ctaaaagctg
     8881 gagtggtggt ataaattatg gaaataaatt taataataat gaacaacaag ttaatggtag
     8941 ttacttgtat tcaaaagtta ttaatgaagg aacaggttat tcaaaatcaa aatcattatt
     9001 acccgatacc gttttcttta acaatgaaaa caacaaaact actggcgtaa aagacaagca
     9061 tggtattaat ggaactgtag attggcaaat ggatagcttg ctatctttaa aagtaactgt
     9121 ttctgcaagt aaaggaaata gctatggcaa cagtaattat tataacgaag ccttaaatga
     9181 acagaaccag tatgtaaaca acagtatcag aaacaaaata aatcagggga agaataagaa
     9241 tataaattat aatgtattgg taagaaaaaa atttaaaaaa ataggaagaa cattatcttt
     9301 aagtttagag caaaagttca acactcaaaa taatgatggt tacttatatt ctgaaaataa
     9361 tttttataat gctaatggaa gtttaaattt ttctgacact acggaccaaa agaaattatc
     9421 ggataataat actaataatt taaaatcaaa aattgcattt acagaacaac ttttaaaaag
     9481 tttagttgtt gaatttagct atacttacca gttaaataat tacaccaaca aagtattaag
     9541 ttatgataaa aacaattctg gaaaatacga agattttaat actaaattta gtaacaactt
     9601 taagtttacc aatgctacaa gtttaattgg caccatgttt agataccaaa aaaataaatt
     9661 aaatgtatct tttggtggaa atgtatcttt agccaacttt aaacaaaaag atttaataaa
     9721 agatacaact taccattacc aattcaacaa tatatatcct accgctattt taacttataa
     9781 atttaactct aataaaagaa ttaccattaa ttataatgga aacaccaagc agccaagtat
     9841 aaaccaacta caacctgttg cagataacaa caacccattg tttatacaaa aaggcaaccc
     9901 taatcttgtt caggaattta accatgcatt tgcagttaat tacaataatt atcaggtttt
     9961 aaaaaacaga ggttatggtt ttagaattag atacaatacc acttataatg caattagaag
    10021 cagcatttat acagatagtt taggaagaag catatcgcaa tatgtaaacc taaacggtaa
    10081 taattacttt accaccaata ttaattacaa cactaaagtt aaaaagttag atatttatgt
    10141 taatgcaagt tactcgttta atattggcaa tagtataagt atagtaaaca accaaaccaa
    10201 taaaaatgta aataaaagcc atcaaatgta tgtaggcatt tctaaagaaa aagaaaacaa
    10261 atttaacttg tggctataca actctattgc ttataatata tcctcttctt ctatacgttc
    10321 tgaaattaat acccaatatt ggtcagcaag tttaggagca gatttgtcgg tttatttacc
    10381 ttggaaacta gaactaaata ataatattga agctgagtta aggcaaaaaa ctgaattatt
    10441 ttcaggtaat aataatatta ctatttggaa tatttatatc tccagaaaaa tttttaaagg
    10501 cgataaaagt cagctacgtt ttactgcatt tgatatatta aatcaaagaa gaaattatga
    10561 aagagatatt agcagcaata ctattactga aactggctat cagcaactat ctcaatattt
    10621 tttattcagt tttatttgga attttaataa aggtggtgct gcaataacaa aataaaaatt
    10681 atatgaaatc aatactatta tcttttattt tttatttttt gtttataaac ttacctgctc
    10741 agcaaatttt tattaacact ggtaaaattg aatttgagaa acaagttaac cttcataaag
    10801 tacttgaatt aagctggggc gatgaggaga atgacaatgt atggataatg aatatgaaaa
    10861 aagaattgcc caaaataaaa tctacttttt ataatttata ttttacccaa aataaaacaa
    10921 tatataaagc aggtaaagaa gccccacaaa ctgcacagaa aataccagag tggctcgctg
    10981 ataatgatgc attagaaaat attacttata ccgatttagc aacccataaa acaattgcaa
    11041 agaaaaaagt ttttgaaact acctttttat tagaagatag tataactaaa ataaattggc
    11101 gtataacaaa tgatacacga accattgcag gcattgaatg tagaaaagca gttggtaaaa
    11161 tttttgacag tgtatatgta attgcttttt atactgattt aataacatgc actggtgggc
    11221 ctttaagctt tacaggttta ccaggtatga ttttaggagt tgcaattcca agaattcata
    11281 caaccttatt tgcaacaaaa gttgaattag caaatattga tgaaacaata tttgccattc
    11341 ctaaatctgg caaaaaaaca agttatgtta atttacttaa gcaattaaaa tcatcaatga
    11401 acgattgggg taaagagggt aatttaatta tatggaaaac cctactttaa tataaattaa
    11461 acacttttaa tttctgttat caatttttgt aaagcagctt ttgcatcgcc aaaaagcatg
    11521 gaggttttag gctgaaaaaa caaatcgttt tcaatgccag cataacctgg tttcatactt
    11581 cttttattaa caataacatt ttttgcttgt tctacttcta aaattggcat accataaatt
    11641 ggcgatgcag gatctttttt agcagccgga tttaccacat cattagcacc taaaactaac
    11701 actacatcgg tacttctaaa ctcttcatta gcatcttcca tttctaataa tttatcataa
    11761 cttacatctg cctctgctaa taaaacattc atgtggcctg gcatacgacc tgcaacaggg
    11821 tgtattgcat atttcacttc tacaccttta tcttctaata atttttcaag ttcgtgacat
    11881 gtatgttgag cttgggcaac agccaaacca tagcctggta caatcattac tttattggca
    11941 aatgccaaaa ttgttgcagc atcgctaagc tgtatttctt tgaacgagcc ttgttctttg
    12001 cttcctgctg caccagcttt gttaccacca aaagagccta ataacacatt cttcaaactt
    12061 ctattcatag ctttacacat taatatggta agcaatgtac ctgcagcacc tactaaaata
    12121 cctccagtta gcattacttt attgtcatat aaaaagcctc cacaagcagc tgcaacacca
    12181 gtaaaagagt tgagtaaaga tattactaca ggcatatctg caccacctat tggcataaca
    12241 aataaaacgc catataaaag tgatacaaca aatatgatat aaaaccaata agcattggtt
    12301 aatgaaagtg aaaataccaa atacccggca atacctaaaa ttactgccat taaacctaca
    12361 tttataaact gttgcccatt gtaggaaaaa tctttaattg taccattaag tttaccccaa
    12421 gcaatcatac tacctgcaaa agaaactgaa ccaataattg cccctaaaaa tatacttatt
    12481 aataagccat aattggcagt aacattatta tttaagtgat gagcaatatg ctgaaactca
    12541 ataatagaaa ttaatgctgc acaagcccca cccataccat taaataagct taccatttct
    12601 ggcattgcag tcatttttac ttttttagct attaaagtac ctaccacagt accaattgcc
    12661 aagcctccaa atatccaaaa gtaattgcct aattttttcc cttcttcttc atataaaaaa
    12721 atggtgccaa caattgcaat tgccatacct gcagcagcta caaggtttcc ttttctggca
    12781 cttgcagggt gcgataacat ttttaaacca ataataaaag tgatacttcc tattaaataa
    12841 catattgtta ataccataaa aattaaaatt aaatttctta caattgatta atcaaacttt
    12901 ataaaataat catttctttt tcttaaacat ttctaacatt ctatctgtta caacaaagcc
    12961 accaactaca tttaaagttc ctaaaataac tgctaaaaag cctaacacta aagcaaggta
    13021 attatctgct tctgcttttc ccattactat tattgcacca ataattacaa caccatgaat
    13081 ggcattggca ccactcatta aaggtgtgtg caatacactt ggcactttac ctattacttc
    13141 aattcctaaa aaaatcatta gcacaactat gtaaatgatt tgctcatgtt ctttaataaa
    13201 gtttaagata ttttccattt ttattttgaa ttaattttta aaaataaaat aggctaatca
    13261 attaacctat tgtgaacgat tgaaccatta tgagtaatac atgtgccttt cactaaatca
    13321 tcttcaaaat ttaagttaag tgcaccttca tttgtaataa ttaattgtaa aaagttaaga
    13381 atattttttc catacatttt gcttgcgtca aaaggcatac cacaaggtaa attactgttg
    13441 cctacaatga gaacaccatt ttctgttgta aagttttcat tattttttg
//
LOCUS       DAOQPK010000036        13483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_2362_length_13483_cov_4.646038, whole genome shotgun sequence.
ACCESSION   DAOQPK010000036 DAOQPK010000000
VERSION     DAOQPK010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 13483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13483
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2362_length_13483_cov_4.646038"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PLU36_03025"
     CDS             complement(<1..496)
                     /locus_tag="PLU36_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit beta"
                     /protein_id="HMZ45756.1"
                     /translation="MKKEKEEDLDINLTGDGVTTSSSNTEVKTKWYERRRKGITTDTS
                     DKKETPEGLWNKCPVCSFIQPTVELCDNKFVCPKCNYHHRIGSKEYFEYIFDAGTYSR
                     LFDEIKSKDFLGFTDLKPYQKRLDEIHAKTDLKDSMSVATGTVNGKGLVIACMDFEFI
                     GGSLG"
     gene            complement(653..1723)
                     /locus_tag="PLU36_03030"
     CDS             complement(653..1723)
                     /locus_tag="PLU36_03030"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HMZ45757.1"
                     /translation="MATNIQQILGDKAEYLLNHTSKTIDKSQLHLPGADFVDRIFMQS
                     DRNVNVLKSLQSMYSTGRLANTGYLSILPVDQGIEHSAGASFAKNPIYFDPENIVKLA
                     IEGGCNAVATTYGGLGFLARKYAHKIPFIVKINHNEFLTYPNKFDQVMFASLKASQNL
                     GATAVGATIYFGSDESTRQIQEVSKAFEMAHELGMATILWCYLRNSSFKKDGVDYHVS
                     ADLTGQANHLGVTIQADIIKQKLPENNGGYNALNSKENPYGKTDKLVYSNLTTEHPID
                     LCRYQVANCYMGRAGLINSGGASSGASDLAEAVTTAVVNKRAGGMGLISGRKAFQRPM
                     KEGIGILNAIQDVYTDSSITIA"
     gene            complement(1828..2613)
                     /locus_tag="PLU36_03035"
     CDS             complement(1828..2613)
                     /locus_tag="PLU36_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HMZ45758.1"
                     /translation="MLENNQHIKLENSLKIISLVPSQTELLYYLGLEEEVVGITKFCV
                     HPNHWFKSKTRVGGTKNIDFNKIKLLQPNLIIANKEENVKEQIEELQQYFNVIVTDVN
                     NVEESYEMISTIGELTNTATKATQLIQQIKECFFQLKLAYKNTVRPSVCYLIWKEPYM
                     TIGGDTFINDILNWAGFKNVFEQKNRYPQISLEELEKLQPDYIFLSSEPYPFKEQHKQ
                     ELLKNHPTIQSKIILVDGEMFSWYGSRLLQSSNYLKKLILELP"
     gene            complement(2607..4034)
                     /locus_tag="PLU36_03040"
     CDS             complement(2607..4034)
                     /locus_tag="PLU36_03040"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HMZ45759.1"
                     /translation="MPHESISVFDMFKIGVGPSSSHTLGPWKAALLCLDTINLKIGIE
                     KVTSVTVLLYGSLAKTGKGHGTDIAVILGLCGENPETIDVNSITPTIQKINTENIIKL
                     GGKQNIHFNPSENIKFLYNESLPYHPNGMTINVFTEEGQVLSETYFSIGGGFVEKEGQ
                     ENVSSSLVDLPFPINTSNDLEYWCMKTGMHIYDVVLENENTWRTEEETKKKLLKIWHT
                     MRDCIYRGCHAQGELPGGLKVKRRAFNLNKKLTEGRSYNSYEEWQKVIQQGGISFQYI
                     LDWVSCFALAVNEENAGFGRVVTAPTNGAAGVIPAVLQYYITFCNGGTDEQIIRFLLT
                     SAEVGSIFKKGATISAAMGGCQAEIGVSSAMAAAALTECLGGTQKQALMAAEISMEHH
                     LGLTCDPIGGLVQVPCIERNTMGAIKAITASQLALKSTPEFAKVSLDKVIKTMWETAL
                     DMSSKYKETADGGLAVNVPLSLSEC"
     gene            4229..4738
                     /locus_tag="PLU36_03045"
     CDS             4229..4738
                     /locus_tag="PLU36_03045"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HMZ45760.1"
                     /translation="MKIFLIGMMGSGKTYLSKLIADKIKFTFYDLDQIIEKRAGVEIN
                     NIFEEQGELYFRKIETEELGKFENKNNLVLATGGGTPCFNNNMNWMNKHGITIWLNEP
                     LDIICERLMKEKATRPLLKNKKNIEPYLKELLASRIDFYKKATYHFTYSDYNFNNLIS
                     TINQLKENE"
     gene            4731..4991
                     /locus_tag="PLU36_03050"
     CDS             4731..4991
                     /locus_tag="PLU36_03050"
                     /inference="COORDINATES: protein motif:HMM:NF016156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HMZ45761.1"
                     /translation="MNNTILKLAAFLGALSVALGAFAAHGLKKLISEKAVETFQTGVT
                     YQFYHVFALLAAGILFRYYKNKFIQNASIFFLLGILLFSGSL"
     gene            complement(5173..6159)
                     /locus_tag="PLU36_03055"
     CDS             complement(5173..6159)
                     /locus_tag="PLU36_03055"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HMZ45762.1"
                     /translation="MRSIAFREALREAMSEEMRRDERVFLMGEEVAEYNGAYKVSQGM
                     LAEFGAKRVIDTPIAELGFAAIAVGAAQNGLRPIVEFMTWNFAVLPLDQIFNTASKML
                     AMSGGQIKCPIVFRGPNASAGQLGAQHSSAFESLLANIPGIKVVSPSNGYDAKGLLKQ
                     AIRYEEDPVCFLESEVMYGDKSEIPDEEYYIPLGKADVKKIGKDVTIVSFNKMMKVAL
                     NAATELEKEGISAEVIDLRTIRPLDWQTILESVKKTNRLVIVEEQWPFASVSSEITYR
                     IQKEGFDYLDAPIRRITSADAPMHYAPNLVEAYLPSVERTVKLVKEVMYLQA"
     gene            complement(6267..7511)
                     /gene="hutI"
                     /locus_tag="PLU36_03060"
     CDS             complement(6267..7511)
                     /gene="hutI"
                     /locus_tag="PLU36_03060"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HMZ45763.1"
                     /translation="MSATLITNIKFLVNATETPALLKGKDLANLPVLENAYLIISNKK
                     IVAYGVMEDFLHHTQTIKEVIDAEGQMVLPCWCDSHTHLVFAASRENEFVDKLKGKTY
                     AEIAASGGGILNSAKRIAYSTEQELFDSAYNRLHELIKLGTGAIEIKSGYGLSVESEL
                     KMLRVIKKMKEKSPIPIKATFLGAHTYPLKFKEDHKGYIDLIINEMLPAIAKENLADY
                     IDVFCEEGFFSKEETITICKAGMQYGLKPKIHANQLNLSGGVQAGVELNALSVDHLET
                     MNDEAINLLAKSNTIGTLLPTAAYFLRMPFQPARKLIDAGCTIALASDFNPGSSPSGN
                     MNFVVSMSCIQMKMLPEEAINAATINGAAAMELQNEVGSITKGKIANLIFTKPIPSIA
                     YMPYAFGSNLIDKVMINGSFYK"
     gene            complement(7564..8292)
                     /gene="bshB1"
                     /locus_tag="PLU36_03065"
     CDS             complement(7564..8292)
                     /gene="bshB1"
                     /locus_tag="PLU36_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HMZ45764.1"
                     /translation="MKLDILAFGVHPDDIELGCAGTLLAAMAEGKKVGIIDLTQGELG
                     TRGTAETRKVEAENAAKILNVQIRENLKMADGFFKNDEAHQRKVIETIRKFKPEIVLC
                     NAPEDRHPDHGRSSKLVSDASFLSGLIKIVTNDNGKLQEAWRPKYVFHYIQDRFLQPD
                     FVVDISAHQATKMQAIACYTTQFFNTDSDEPQTYISNPVFLETVKARAMMLGKRIGVE
                     YAEGFISEKVIGINSFDAFIQNVT"
     gene            complement(8381..8632)
                     /locus_tag="PLU36_03070"
     CDS             complement(8381..8632)
                     /locus_tag="PLU36_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HMZ45765.1"
                     /translation="MKQGIHPESYRLVIFKDMSNGHAFLSKSTAATKETLKWEDGNEY
                     PLIKLDISNTSHPFYTGKNVMVDTAGRIDKFKKRYEKKK"
     gene            complement(8762..9700)
                     /locus_tag="PLU36_03075"
     CDS             complement(8762..9700)
                     /locus_tag="PLU36_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45766.1"
                     /translation="MKWIVVLIGCCCNNILHAQDSVIVIDKKKFTLTEVVVRNNFDYK
                     KILQQIKEDTTFYKAFKNLRILGYTAFNDIKMLNKKDKIIASLFSKTIQTRNNNCRTM
                     QVLEENVTGNFYDKNGNFNYTTANMYASLFFTNGTVCNENNIVAGNTFSTANKKGMEK
                     HKEQLKMLFFNPGKKIPGIPFIDNKIDLYDNAAQKIYNYTLDLQEKGGKIYYVFSIIP
                     KEKLGLFKADNVVIDEMVTWFDQKTMEVVYRSYSLSYNAGVYDFDVNMEVEMTNHNGY
                     IVPKTLRYRGNWDVMFKKREKALFTATLFNFKSEGE"
     gene            complement(9697..10275)
                     /gene="def"
                     /locus_tag="PLU36_03080"
     CDS             complement(9697..10275)
                     /gene="def"
                     /locus_tag="PLU36_03080"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HMZ45767.1"
                     /translation="MIFPIVAYGASVLRKKALAIDSQYPQLNTLISNMWETMYASNGV
                     GLAAPQINKDIRLFVIDSSQIFENMEEAEKKNYPDSPGLKKVFINAQIKDLDGEEWSF
                     NEGCLSIPKIREDILRPEMVVLEYMDENFETHIEAFHGITARVILHEYDHIEGKLFID
                     YLKPLKKKLLQGKLNDIMKGKVKVDYKMTFIK"
     gene            complement(10329..10742)
                     /gene="ruvX"
                     /locus_tag="PLU36_03085"
     CDS             complement(10329..10742)
                     /gene="ruvX"
                     /locus_tag="PLU36_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HMZ45768.1"
                     /translation="MARIICIDYGAKRSGIAVTDPLQIIATALTTINTSNLLAFLQNY
                     FTQEEVELILVGEPLGLNDEATHATPLVYAFVKNLKKQFPLIPVKLYDERFSSKMAVQ
                     AMIEMGMKKKERKIKGNIDQIAATIMLQEYLANKT"
     gene            complement(10754..11233)
                     /locus_tag="PLU36_03090"
     CDS             complement(10754..11233)
                     /locus_tag="PLU36_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45769.1"
                     /translation="MKKLFTFLLVTLVVFSSNSLKAQDWKLGVSIMPGVASNGGYGFV
                     LGSDARLQTKLMPKMHFILTTGVTQYFKKSPLSGFGYIPLKPGVKYFWGDNFYTAGEI
                     GLGFGLVKGSGRSFIWSPSVGLAFKKVDVSLKYEDTPDFGRYGKHFAIRVARGFDLK"
     gene            complement(11293..12204)
                     /locus_tag="PLU36_03095"
     CDS             complement(11293..12204)
                     /locus_tag="PLU36_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HMZ45770.1"
                     /translation="MPTVKNYLSLIKFSHTIFALPFAMIGFVLGIKTVDEFNKSLLIG
                     KLQLNNFWSFFAIKLLLVLVCMVSARSAAMAFNRYLDRNFDSKNPRTAIREIPSGIIA
                     PNSALRFVIVNCLIFITATFFINDICLYLSPIALLVILFYSYTKRFTALCHLVLGIGL
                     SLAPIGAYLAITGAFSFIPLLFSFAVIFWVSGFDIIYALQDDEFDKAYKLHSIPAALG
                     KKKALKVSELLHFFCTICVIWAGWLGSFSWLYWLGVCIFIAMLVYQHSIVKPNDLSKV
                     NIAFMTANGIASVVFAVFVIADLLILA"
     gene            complement(12266..13282)
                     /gene="fbp"
                     /locus_tag="PLU36_03100"
     CDS             complement(12266..13282)
                     /gene="fbp"
                     /locus_tag="PLU36_03100"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HMZ45771.1"
                     /translation="MNINRNILTLDEFTIQQLRDFPAATGELSSLLRDLGLASKRVNV
                     EVNKAGLVDILGDFGTTNVQGEEVKKLDVYANNQFMGALKHGISCAGIGSEELDDIVI
                     FDDPVSNNSKYVCLFDPLDGSSNIDVNVSIGTIFSVYRRVSELGKPCTKEDFLQPGNK
                     QVAAGYVIYGSSTMFVYATKRGVNGFTLDPQIGEFCLSHPDIKCPAKGKIYSVNHGNF
                     FQYEQGVKEYITACQKKTKADGGPYTQRYIGSMVSDVHRNLIKGGIFTYPGTTDKPKG
                     KLRLLYECNPFAFIIEKAGGKATNGKQRILDIQPTELHQRTPFFIGSSSMMDELETYL
                     AANK"
BASE COUNT         4358 a         2325 c         1849 g         4951 t
ORIGIN      
        1 ttcctaaact tccacctata aattcaaaat ccatacatgc aattactaac cctttgccat
       61 taacagtacc tgtagcaaca ctcatgctat cttttaaatc agtttttgca tgaatttcat
      121 ctaatctttt ttggtaaggt tttaaatcgg taaatcctaa aaaatcttta ctttttattt
      181 catcaaaaag tctagagtag gtgcctgcat caaaaatata ttcaaaatac tctttgctgc
      241 caattctatg atgataatta cattttgggc aaacaaattt attatcgcat aattccacag
      301 ttggttgaat aaagctacaa acagggcatt tattccataa accttctgga gtttcttttt
      361 tatcagatgt atcggtagtt attccttttc tacgtctttc gtaccatttg gttttaactt
      421 ctgtattact tgatgaagtt gttacaccat cgccagttag gtttatatct aagtcttctt
      481 ctttttcttt tttcattgct ctaagagctt tagtttggat aatgtgaaga taaggaaatt
      541 gatgatttta aaaacaaata accacgaatg gagctaattt attgaataat actttattta
      601 tcactcaatt taagaacaac atacgtggtt attaaatttt attttcaatt gcttatgcaa
      661 tagttatgct gctatctgta taaacatctt gtattgcatt taaaattcca ataccttctt
      721 tcattggtct ttgaaacgct tttcttccac taattagccc cattcctcct gcacgtttgt
      781 ttacaacagc tgttgttact gcttctgcta aatcgcttgc accactactt gcaccaccac
      841 tatttattaa accagcacgg cccatataac aatttgccac ctgataacga caaaggtcaa
      901 ttggatgttc cgtagtaagg ttagagtaaa ctaatttatc ggttttgccg tatgggtttt
      961 ctttagagtt taaagcgtta taaccaccat tgttttctgg tagtttttgt ttaataatat
     1021 ctgcctgaat agttacacct aagtggtttg cttgcccagt taaatcggca ctaacgtgat
     1081 aatcaactcc atctttttta aatgaagagt tacgtaagta gcaccataaa atggttgcca
     1141 tgccaagttc gtgagccatt tcaaaagctt tgcttacttc ttgtatttgg cgagtgcttt
     1201 catcgcttcc aaaataaata gttgccccta ctgctgtagc accaaggttt tggcttgctt
     1261 ttagagaagc aaacattacc tgatcgaatt tatttggata agttaaaaac tcattatgat
     1321 ttattttaac aataaaagga attttatgag catattttct ggctaaaaag cctaatccgc
     1381 cataagttgt agcaacagca ttacaaccac cttctatagc taattttaca atattttctg
     1441 gatcaaaata aataggattt tttgcaaaag aagcaccagc actatgttca ataccttggt
     1501 caacaggtaa aatagataaa taccctgtat ttgctaaacg tcctgtactg tacatagatt
     1561 gtaaactttt taacacattt acatttctat cgctttgcat aaatattcta tctacaaaat
     1621 cagcacctgg taaatgtagt tgagacttgt cgatagtttt acttgtgtgg tttagtagat
     1681 actcggcttt gtcgcccaaa atttgttgaa tgtttgttgc catattagtt ttttttgttt
     1741 tgcagaaaaa tagaggtttg caaatataaa gcaaagcatc attcaaagta attggaggct
     1801 ataaaatacc aactttatac actactttta tggaagttca agtattaatt ttttaagata
     1861 gttacttgat tgtaatagcc tgcttccgta ccaactaaac atttctccat ctactaaaat
     1921 aattttagat tgaatagttg gatgattttt tagaagctct tgtttatgtt gttctttaaa
     1981 agggtaaggt tctgaagata aaaaaatata atctggttga agtttttcta attcttctaa
     2041 agaaatttgt gggtatctat ttttttgttc aaaaacattt ttaaacccgg cccagtttaa
     2101 aatatcattg ataaaagtat ctccaccaat ggtcatatat ggttctttcc aaattaaata
     2161 acaaacactc ggccttacag tatttttata tgctaattta agttgaaaaa aacattcctt
     2221 tatttgctga attaattgag tagcttttgt tgctgtattg gttagttctc caatagtact
     2281 aatcatttca taagattctt ctacattatt tacatcggta actattacat taaaatattg
     2341 ctgcagttcc tctatttgct cttttacatt ttcttcttta ttggctataa ttaaattagg
     2401 ttgaagtagt tttattttat tgaaatcaat attttttgtt ccgcctactc ttgttttgct
     2461 tttaaaccaa tggttagggt gaacacaaaa ttttgtaata ccaactactt cttcttccaa
     2521 tcctaaataa tacaataact cagtttgaga gggaactaat gatataattt ttaaagaatt
     2581 ttcaagttta atatgttggt tattttctaa cattcactta aactcaacgg tacatttaca
     2641 gctaaaccac catctgcagt ttctttgtat ttactgctca tgtctaatgc tgtttcccac
     2701 attgttttaa ttactttatc taaacttact ttagcaaatt ccggagtgct ttttaatgct
     2761 aattgagagg ctgtaatggc tttaatagca cccattgtat ttctttcaat acaaggcact
     2821 tgaacaaggc caccaatggg gtcgcaggtt aagcctaaat gatgttccat tgaaatttca
     2881 gctgccatta atgcttgttt ttgtgtacct cctaaacatt cagttaatgc agctgcagcc
     2941 attgcagatg aaacaccaat ttctgcctgg cagcctccca tagcagcaga aatagtggca
     3001 ccttttttaa atatacttcc tacttcggca gaggttaata aaaaacgaat aatttgttca
     3061 tcggttcctc cattacaaaa agtaatataa tattgcaata cagctggtat aacgcctgct
     3121 gcaccattag ttggtgctgt tactactctt ccaaaaccag cattttcttc gtttactgcc
     3181 aatgcaaagc aacttaccca atctaaaata tactgaaagc taatgcctcc ttgttgtata
     3241 actttttgcc attcttcgta agaattgtat gatcttcctt cagttagttt tttatttaaa
     3301 ttaaatgccc ttctttttac ttttaaccca cctggtaatt caccttgagc atggcaacct
     3361 ctataaatac aatctctcat ggtgtgccaa atttttagaa gctttttttt ggtttcttct
     3421 tctgttctcc aagtgttttc attttctaaa actacatcgt atatatgcat tcctgttttc
     3481 atacaccaat actctaaatc gtttgatgta ttgataggaa aaggtaaatc tactagtgat
     3541 gaactaacat tttcctgtcc ttctttttct acaaaccctc caccaataga gaaataagtt
     3601 tcgcttaaca cttggccttc ttctgtaaaa acatttatag tcattccatt gggatgataa
     3661 ggcaaagatt cgttgtacaa gaattttata ttctctgaag gattaaagtg aatgttttgt
     3721 tttccgccta gtttaataat attttctgtg tttatttttt gaatagttgg tgtaatacta
     3781 tttacatcaa ttgtttcagg attttcgcca cataagccta aaataacagc aatatctgta
     3841 ccatgccctt tgccagtttt agctaaagaa ccataaagta aaacggttac actggttact
     3901 ttttcaattc caattttaag atttatagta tctaaacaaa gtaaggctgc tttccatggg
     3961 ccaagtgtgt ggctgcttga aggaccaaca ccaattttaa acatatcaaa aaccgaaata
     4021 gattcgtgtg gcatactatt atttgaaaca aatatacaca agcttttgtt tagtaattta
     4081 ccaatttgaa taattcgaat atctgaacaa attgtatgat aattgtacac atttgaaagt
     4141 agtcttgttt actggttgta ctaataattt aaagctaaat gtatattttt attcttttaa
     4201 aagaaattac tagttgtata attaacaaat gaaaatattt ttaattggca tgatgggcag
     4261 tggtaaaact tatttaagta agctaattgc ggataaaata aaatttactt tttatgattt
     4321 agaccaaata attgaaaaaa gggctggtgt tgaaataaac aacatttttg aagagcaagg
     4381 tgaactttat tttagaaaaa ttgaaacaga agagttaggc aaatttgaaa ataaaaataa
     4441 tttggtttta gcaactggtg gtggtacacc atgttttaat aataatatga attggatgaa
     4501 taagcatgga attacaattt ggttaaatga gcctttagat attatttgtg agagattaat
     4561 gaaagagaaa gcaaccagac ctttgttaaa gaataaaaag aatatagaac cttacttgaa
     4621 agaactatta gcctcacgaa ttgattttta taaaaaagct acttaccatt ttacctactc
     4681 tgattataat ttcaataatt taatttcaac aattaatcaa ttaaaagaaa atgaataata
     4741 caatattaaa acttgcagca tttttaggtg cattaagcgt tgcattaggg gcttttgcag
     4801 cacatggttt aaaaaaatta attagcgaga aagcagtaga aacttttcaa actggtgtta
     4861 cttaccaatt ttatcatgta tttgctttgc ttgcagctgg tatattattt aggtattaca
     4921 aaaataaatt tatacaaaat gcttctatat ttttcctttt aggaattttg ctttttagtg
     4981 gttctcttta acttttaaca ctaaaagaat atttgcaatt agaaagttta caatgggttg
     5041 tatttattac accttttggt ggtgtttgct ttatggttgg ttggatgttt ttattgttag
     5101 gaataaaaga aaattaaaaa acctatcaac ttgtaaagtg ataggtttta aataagtagt
     5161 tcaactaaaa tattatgctt gtaaatacat tacctctttt accaacttaa cagttctttc
     5221 aacactaggt aaatacgctt ctaccaaatt aggtgcatag tgcattggtg catctgcact
     5281 tgtaattctt cttataggag catctaagta atcaaatcct tctttttgaa ttctataagt
     5341 tatttctgag cttacacttg caaatggcca ttgttcttct actataacta aacggttggt
     5401 ttttttaaca ctttctaaaa tggtttgcca gtctaatgga cgaatagttc ttaagtcaat
     5461 tacttcggca ctaatacctt ctttttctaa ttctgtagcg gcatttaaag ctactttcat
     5521 cattttatta aatgatacaa tggttacatc tttcccaatt tttttaacat ctgctttacc
     5581 aagtggaata taatattctt catctggaat ttcagattta tcaccataca ttacttcgct
     5641 ttctaaaaaa caaactggat cttcctcata tctgatagct tgttttaata atccttttgc
     5701 atcataccca ttgctaggcg aaaccacttt tatacctgga atatttgcta ataaactttc
     5761 aaaggcagag ctatgttgtg cacctaactg gcctgcactt gcattagggc cacgaaaaac
     5821 gatagggcat ttaatttgcc caccactcat agccaacatt ttagaggctg tattaaaaat
     5881 ttgatcaaga ggtaacactg caaagttcca tgtcataaac tctactatag gtcttaaacc
     5941 attttgtgca gcccctacag caattgctgc aaaaccaagt tcggcaatag gggtatcaat
     6001 tactcttttg gctccaaact cggcaagcat accttggcta actttataag caccattgta
     6061 ttcggcaact tcttcaccca ttaagaaaac tctttcatct cttctcattt cttcactcat
     6121 ggcttctctt aatgcttctc taaatgctat tgaacgcata ataattttat attaaggtgt
     6181 ttgcaaaagt aattgattag tgcatataat ccaatgtagt attattgagt ttgaaatact
     6241 ttctaaataa agttagaaat ggtttattat ttataaaaac ttccatttat cattaccttg
     6301 tcaattaaat tacttccaaa agcataaggc atataagcta ttgatggaat tggtttggtg
     6361 aaaattaagt tggcaatttt tcctttagta atactgccaa cctcattttg cagttccatt
     6421 gctgcagcac cattaatggt agctgcatta attgcttctt ctggtagcat tttcatttga
     6481 atacagctca tagatactac aaaattcata ttgccacttg gagatgaacc aggattaaaa
     6541 tcacttgcaa gtgcaatggt acaacctgca tcaattaatt ttctggcagg ctgaaaaggc
     6601 attcttaaaa aataagctgc tgttggtaat aatgtaccta ttgtatttga tttagctaat
     6661 aagtttattg cttcatcatt cattgtttct aaatggtcta ctgaaagggc atttaattct
     6721 acacctgctt gtacgccacc acttaggttt aattggtttg catgaatttt aggctttaaa
     6781 ccatattgca taccagcttt gcagatggta atagtttctt ctttactaaa aaatccctct
     6841 tcgcaaaaca catcaatata atctgccaaa ttttctttag caatggcagg aagcatttcg
     6901 ttaataatta aatcaatata gcctttatga tcttctttaa atttaagtgg ataagtatgt
     6961 gcccctaaaa aagttgcctt tattggtata ggagattttt ctttcatttt ttttataaca
     7021 cgaagcattt taagttcact ttctacactt agtccataac cacttttaat ttctattgca
     7081 cctgtgccta attttattaa ttcatgcaac ctattataag cagagtcaaa aagttcttgc
     7141 tcggtagagt aagcaattct tttagcagaa tttaaaattc caccaccact tgcagcaatt
     7201 tctgcatagg ttttgccttt taatttatct acaaattcat tttctctact tgctgcaaat
     7261 actaaatgtg tgtggctatc gcaccaacaa ggtaaaacca tttgtccttc tgcatctatt
     7321 acttctttaa tggtttgtgt atgatgtaaa aaatcttcca ttacaccata agcaactatt
     7381 tttttgttag aaataattaa atatgcattt tctaaaacag gcagatttgc taaatctttt
     7441 ccttttaata aggctggtgt ttcggttgca ttaaccaaaa attttatatt ggttattaag
     7501 gtagcagaca tattcttata cactttttat aatgataaat caattttcaa attaattgta
     7561 atattaagtt acattttgaa taaaggcatc aaaagaattt attcctatta ctttttcaga
     7621 tataaatcct tctgcatact ctacaccaat tcttttcccc aacatcattg cccttgcttt
     7681 aacagtttct aaaaatactg ggttagaaat atacgtttga ggttcatcag aatctgtatt
     7741 aaagaattgt gtagtataac aagcaatggc ttgcattttt gttgcctgat gtgcagatat
     7801 atctactaca aaatcaggtt gcaaaaatct atcttgtata taatgaaaca catatttagg
     7861 acgccaggct tcttgtaatt taccattgtc gtttgtaaca attttaatta aaccgcttaa
     7921 aaaagatgca tcactaacta atttggagct tcttccatga tctgggtgcc tatcttctgg
     7981 tgcattgcac aatactattt ctggtttaaa ttttcttatt gtttcaataa cttttctttg
     8041 atgagcttca tcattcttaa aaaagccatc ggccattttt aagttttctc ttatttgtac
     8101 atttaaaatt ttggctgcat tttcagcctc cacttttctg gtttctgctg tacctcttgt
     8161 tcctaattct ccttgagtta aatctataat accaactttt ttaccttctg ccattgcagc
     8221 taataatgtt cctgcacagc ctaattcaat atcatcgggg tgtacaccaa atgctaaaat
     8281 atctaatttc atttattcta aaattttgat aaaaataatg aaaaagaaaa atcccgtttt
     8341 taggaacggg atttttctaa aataaaaaaa acaatgaaaa ctattttttc ttttcgtaac
     8401 gttttttgaa tttatcaata cgtcctgcag tgtcaaccat tacattttta ccagtgtaaa
     8461 aaggatgaga tgtattagag atatctaatt taattaaagg atactcatta ccatcttccc
     8521 atttaagtgt ttctttagtt gcagcagttg atttgcttaa aaatgcatgc ccattactca
     8581 tgtctttgaa aattactaaa cgataacttt ctggatggat accttgtttc ataaattttt
     8641 ttttaaggct gcacggattt tgccgtgtca attaaataat aatagtttgc aaatttaggg
     8701 gctacaacat tcatatccaa aaaggtatag aaatagctga gaatcaatat taaaataagt
     8761 gctattctcc ttcgctttta aagttgaata aagttgcagt aaataatgct ttttctcttt
     8821 ttttaaacat tacatcccaa ttccctctat atcttaatgt tttgggtaca atataaccat
     8881 tatggttggt catttctact tccatattta catcaaaatc ataaacccct gcattataac
     8941 ttaaagaata gctcctatac accacttcca tggttttttg atcgaaccaa gtaaccattt
     9001 catcaataac aacattatct gctttaaaca accctaattt ttctttagga ataatagaaa
     9061 atacataata aattttccct cctttttctt gaagatctaa agtataatta taaatttttt
     9121 gtgcagcatt atcgtataaa tcaattttat tgtcaataaa tggtattcct ggaatttttt
     9181 tacctggatt aaaaaataac attttaagtt gttctttatg tttctccata ccttttttgt
     9241 ttgcagtaga aaaggtatta cctgcaacta tattgttttc attacaaact gttccatttg
     9301 taaaaaataa cgaagcatac atattagctg ttgtataatt aaagttgcca tttttatcgt
     9361 aaaaattacc tgttacattc tcttctaaca cttgcatagt tctgcaatta ttatttcttg
     9421 tttgtatggt tttactaaac aacgaagcaa taattttatc ttttttattc aacattttaa
     9481 tatcgttgaa cgcagtgtaa cctaatatac gtaaattttt aaaggcttta taaaaagtag
     9541 tatcttcttt aatttgttgc agaatttttt tgtagtcaaa attatttcta actacaactt
     9601 ctgttaaagt gaattttttt ttatcaatta caatcaccga atcctgagca tgtaggatat
     9661 tattacagca acaacctatt aaaactacaa tccatttcat ttaataaatg tcattttgta
     9721 atctactttt actttgcctt tcatgatatc gtttaacttg ccttgtaata gttttttctt
     9781 taaaggcttt aaataatcta taaataattt accttctata tggtcgtatt catgtaaaat
     9841 aaccctagct gtaataccat gaaaagcttc tatatgtgtt tcaaaatttt catccatata
     9901 ttctaacaca accatttctg gtcttaaaat atcttctctt attttaggaa tagataaaca
     9961 gccttcgtta aacgaccact cttctccatc taaatctttt atttgtgcat ttataaatac
    10021 tttttttaaa ccaggagaat cagggtaatt cttcttctct gcctcctcca tattttcaaa
    10081 aatttgagaa ctgtcaatta caaaaagcct gatatcttta tttatttgag gggcagctaa
    10141 accaacgcca ttactagcat acatggtctc ccacatattg ctgattaaag tatttaattg
    10201 tggatattgg ctatctattg ctaaagcctt ttttcttaat acacttgcac cataagccac
    10261 tatgggaaaa atcatttgtt aaactatttt atatttataa cttttaatta ttttaaaaaa
    10321 gagctatatt atgttttgtt agccaaatat tcttgcaaca ttatagtagc agctatttgg
    10381 tctatattgc ctttaatttt tctttctttt tttttcattc ccatttcaat cattgcttga
    10441 acagccattt tagaactaaa cctttcatcg taaagtttaa ctggtattaa tgggaattgt
    10501 ttttttaaat tttttacaaa tgcataaacc aatggtgttg catgtgttgc ttcatcattt
    10561 aaacctaaag gttcgccaac caatattaat tcaacttctt cttgggtaaa ataattttgc
    10621 aaaaatgcaa gtaggttact tgtatttata gttgttaatg ctgttgcaat tatttgtaat
    10681 ggatcggtaa cagcaatacc agatcttttg gcaccataat caatacaaat aattctagcc
    10741 attagttgta aaattatttt aaatcaaaac cccttgctac tcttatagca aagtgtttcc
    10801 catatcgtcc aaagtctggt gtatcttcat atttcaagct aacatctacc tttttaaatg
    10861 ccaacccaac agatggagac caaataaaag atctaccgct accttttact aaaccaaaac
    10921 ctaaaccaat ttcgccagca gtataaaaat tatcgcccca aaagtattta acacctggtt
    10981 ttaaaggtat atagccaaag ccagatagtg gacttttttt aaaatattga gttaccccag
    11041 tagttaaaat aaaatgcatt ttaggcatta gcttggtttg taaccttgca tcagaaccca
    11101 aaacaaatcc atatcctccg tttgatgcaa caccaggcat aatactaacc cctaatttcc
    11161 aatcttgtgc ttttaatgaa ttggaagaaa atactactaa cgtaacaagt aaaaaagtaa
    11221 acaatttttt catatcaatt tattataaga ttaacggcga aagtacattt tttttagaaa
    11281 atgtaaaaag tattaagcta aaatcaataa atcggcaata acaaatacag caaaaacaac
    11341 acttgcaatg ccattggcag tcataaatgc aatgtttact ttacttaagt cgttgggttt
    11401 aacaatagaa tgttggtaca ctaacattgc aataaaaata catacaccta accaatacaa
    11461 ccaactaaaa ctacctaacc aacctgccca aattacacat atagtacaaa aaaagtgtaa
    11521 taattcggat acttttaaag ctttcttttt acctaatgct gctggtatag agtgaagttt
    11581 ataggcttta tcaaactcat catcttgtaa agcataaata atatcaaaac cacttaccca
    11641 aaaaataaca gcaaatgaaa atagtaaagg aataaaacta aatgcaccag ttatagctaa
    11701 ataagctcct ataggtgcta aggataaacc aatgcctaat actagatggc ataatgctgt
    11761 aaatcttttt gtataactgt aaaataaaat taccagtaag gctattggtg ataagtataa
    11821 acaaatatca ttaataaaaa aagtagcagt aataaaaatt aggcaattaa caattacaaa
    11881 ccgcaatgca ctgtttggtg ctataatacc acttggtatt tctctaatgg ctgttcgtgg
    11941 gtttttagaa tcaaaatttc tatctaaata tctattaaat gccattgctg ccgaccttgc
    12001 actcaccata caaaccaata ccaaaagcag ttttatggca aaaaaagacc aaaaattatt
    12061 aagttgtaat ttacctatta aaagtgattt attaaattca tccaccgttt ttatgcccaa
    12121 cacaaaacct atcattgcaa atggtaatgc aaaaatggta tgagaaaact taattaagga
    12181 tagatagttt tttacagtag gcatacataa aaaatagaat gtattctatt gtacaaaaca
    12241 cattctaact gttggtattt tttaattatt tattggcagc caagtaggtt tctaactcat
    12301 ccatcatact actacttcca ataaaaaaag gagtacgttg gtgtaattcg gttggttgta
    12361 tatctaaaat tctttgttta ccattggttg ctttgccacc tgctttttca ataataaatg
    12421 caaacggatt acattcatat aacaaacgta atttaccttt tggtttatct gttgttccag
    12481 gataggtaaa aattccgcct ttaattaaat ttctatgcac atcagatacc atgctaccta
    12541 tataacgttg tgtgtatggc ccaccatcgg cttttgtttt tttctgacaa gcagtaatat
    12601 attcttttac accttgctcg tattgaaaaa agtttccatg atttactgaa taaattttac
    12661 cttttgcagg gcacttaata tcggggtggc ttaagcaaaa ttcaccaatt tgagggtcta
    12721 atgtaaagcc atttacgcca cgtttagtag catacacaaa catagtgcta ctaccataaa
    12781 taacataacc agcagcaact tgtttattac ctggttgtaa aaaatcttct ttagtacagg
    12841 gtttgcctaa ttcactcact cttctataca cactaaaaat agtaccaata gaaacgttta
    12901 catcaatatt actacttcca tctaatgggt caaataaaca aacatatttg ctattgttgc
    12961 ttacagggtc atcaaaaatc acaatatcat ctaattcttc actaccaata ccagcacagc
    13021 ttatgccatg ttttaatgca cccataaact gattattggc atatacatct aattttttaa
    13081 cttcctctcc ttgtacattt gttgtgccaa aatctcctaa aatatctact aatcctgctt
    13141 tattaacttc tacatttaca cgtttagacg ccaatcctaa atctcttaat aacgaactaa
    13201 gttcgccagt tgctgcagga aaatctctta attgttgaat agtaaattca tctagcgtta
    13261 aaatatttct attaatattc ataaggcatt ttttaatagt gaaagtttgt ttatagacga
    13321 tttatttgca aatgtatctt aagcaagtta attttcaatt ttctttaaaa aacaaataag
    13381 tttttactta gctaagttaa agaattgggc atttgttgcg tcgcacactt gtactttcta
    13441 ttcattattc accaaacata ttttattgaa tggttggtat tgc
//
LOCUS       DAOQPK010000037        12860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_2505_length_12860_cov_4.029832, whole genome shotgun sequence.
ACCESSION   DAOQPK010000037 DAOQPK010000000
VERSION     DAOQPK010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12860
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2505_length_12860_cov_4.029832"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..196)
                     /locus_tag="PLU36_03105"
     tRNA            complement(124..196)
                     /locus_tag="PLU36_03105"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(161..163),aa:Gly,seq:tcc)
     gene            complement(289..371)
                     /locus_tag="PLU36_03110"
     tRNA            complement(289..371)
                     /locus_tag="PLU36_03110"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(335..337),aa:Tyr,seq:gta)
     gene            complement(402..474)
                     /locus_tag="PLU36_03115"
     tRNA            complement(402..474)
                     /locus_tag="PLU36_03115"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(439..441),aa:Thr,seq:tgt)
     gene            873..1211
                     /locus_tag="PLU36_03120"
     CDS             873..1211
                     /locus_tag="PLU36_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HMZ45772.1"
                     /translation="MTIEDAQNTVNNWINTVGVRYFNELTNLGILMEEVGELSRIMVR
                     TYGEQSFKESDKEKELADEMADILWVLICLANQTGINLTEAFQKNLDKKNNRDFLRHQ
                     NNKKINPSLE"
     gene            1232..1477
                     /locus_tag="PLU36_03125"
     CDS             1232..1477
                     /locus_tag="PLU36_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45773.1"
                     /translation="MTSPLFITQTIIIYGSAILLIVVATILIKYFYANSVKCKLQEYQ
                     REIAKSHSRILKLEVTNDQLKQKVSELETIIHRPKIA"
     gene            1618..3090
                     /locus_tag="PLU36_03130"
     CDS             1618..3090
                     /locus_tag="PLU36_03130"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HMZ45774.1"
                     /translation="MSIQHNNKFQEIISHAKEYGFVFPSSEIYDGLSAVYDYGPYGAE
                     LKKNIRDYWWKSMTQFNDNIVGIDAAIFMHPTTWKASGHVDNFSDPMIDNKDSKKRYR
                     VDHLIEAKAEELALEGKKEEEYELLKKMDEYLAADDFVALKNIIEEYKIKCSVSGTSN
                     WTDIRQFNLMFKTEFGAVAADNAEDNIVYLRPETAQGIFVNFLNVQKTARMKIPFGIA
                     QTGKAFRNEIVARQFIFRMREFEQMEMQFFVRPGTEDEWYKYWKNERLNWHLSLGLPK
                     EKYRYHDHVKLAHYAKEACDIEYEFPIGFKEVEGIHSRSDFDLSQHQQFSKKKQQYFD
                     NDINPETGKAFGNYIPYVIETSIGLDRMFLLVLSNAYEEETITKEDGSTDTRIVLHIP
                     AKLAPVKLAVLPLLKKDGLPEIAQQLMADCKPYFKCFYEEKDAIGKRYRRQDAIGTPF
                     CVTIDHQTKEDNTVTIRFRDSMKQERIAFNKVRELVMQNL"
     gene            3293..4282
                     /locus_tag="PLU36_03135"
     CDS             3293..4282
                     /locus_tag="PLU36_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMZ45775.1"
                     /translation="MKKIFKFIGYLFAIIIVLLATAVAYIKLALPNVGKATDLKVELT
                     QAKIDRGQYLANHVMVCIDCHSTRDWSLFSGPIVAETEGKGGEIFDEKLGFPGKYVSP
                     NLTPYKLKSWTDGEIFRAITSGVTNKGKALFSLMPYHNYGKLDKNDIEAVIAYLRTLK
                     PIESTQESSTSNFPVNILINTFPSKPNFTTIPNTTNTIEYGKYLTTAAGCYDCHTKQE
                     KGKFVGDDYAGGFEFKFADGTIVRSSNITSDKATGLGSWTVEAFVNKFKVYEDSTFEL
                     PKVNPGNFQTVMPWTMYAGMKKEDLAAIFAYLQTVKAVNNTPEKFTSAKLIKN"
     gene            complement(4684..5430)
                     /locus_tag="PLU36_03140"
     CDS             complement(4684..5430)
                     /locus_tag="PLU36_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMZ45776.1"
                     /translation="MSKYITMLEPWRIGKVIKIEQETANTRRYWIEIEELSNFEFTAG
                     QFVTLDLPIHEQKNKRWRSYSIASSPDGTNVIELVIVLLEGGLGTTYIFNNIEVGSTF
                     TLRGPQGKFVLPENIDKDIFLICTGTGIAPFRSMVNNIFQKNIPHQNLHIIYGCRKYA
                     DVLYRAELEALQEKLPHFYYHPVFSREENIKQGEYKGYVHGVYENLLQQNKQPARFYL
                     CGWKNMIDEAKERIIALGYDKKDIHLELYG"
     gene            5545..6039
                     /locus_tag="PLU36_03145"
     CDS             5545..6039
                     /locus_tag="PLU36_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HMZ45777.1"
                     /translation="MKNLFIIRHAKSSTNFLNNNDFERPLNEQGIKDAALMAKKLLSK
                     GIIIDGIVTSTAIRAATTANYFATAFAINKNNFWNEPTLYNANKKTFYTIINNWQFTL
                     NNIAIFSHNPGITHFANSVTNSKIDNMPPCGVFAIKINCNKWQSFETSEKKFWFFDCP
                     EKIW"
     gene            complement(6047..6874)
                     /gene="folP"
                     /locus_tag="PLU36_03150"
     CDS             complement(6047..6874)
                     /gene="folP"
                     /locus_tag="PLU36_03150"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HMZ45778.1"
                     /translation="MFTLNCKGELLTITKPIVMGIINTSPNSFYTNSQQNSIDSALFK
                     VEQMVQEGAIIIDIGGQSTKPNSLPITVEEELRRTVNVIEAIKKKFSNLIISIDTYNA
                     TVASKAVEAGASIVNDISGGTFDKNMMATVANMNVPYVCMHIKGTPQTMQQNPIYDNV
                     TQEVLSFFIDKINQAKQAGIKDVIIDVGFGFGKTIQHNFQLLKELSVFSILEKPILIG
                     VSRKSTIYKTLGVTPEQALNGTTALHTICLLNGANILRVHDVKEAIECIKLFEMYSQ"
     gene            complement(6943..7026)
                     /locus_tag="PLU36_03155"
     tRNA            complement(6943..7026)
                     /locus_tag="PLU36_03155"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6990..6992),aa:Leu,seq:caa)
     gene            complement(7054..7563)
                     /gene="hscB"
                     /locus_tag="PLU36_03160"
     CDS             complement(7054..7563)
                     /gene="hscB"
                     /locus_tag="PLU36_03160"
                     /inference="COORDINATES: protein motif:HMM:NF019363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HMZ45779.1"
                     /translation="MNYFELFNIPISFNPNLSDVKNKFYALSRKFHPDFFTQATQDEQ
                     QYALEQSALINKAYKILNNQESVLKYILELNNLIEDEEKYKLPPNFLMQVMDLNEQLL
                     DAKLEQDESKVNLVVNEINNFKTSIYEPVKSLLENCKEIIYSREELLQIKDFYYKKKY
                     LNRILAGLH"
     gene            complement(7626..8441)
                     /gene="ispE"
                     /locus_tag="PLU36_03165"
     CDS             complement(7626..8441)
                     /gene="ispE"
                     /locus_tag="PLU36_03165"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HMZ45780.1"
                     /translation="MIVFPNCKINLGLNIISKREDGFHNLETIFYPLMIKDCLEILPT
                     HKKENNFTITGIPINVDSSQNICSKAWQLLKLNFPQLPNIQLHLHKTIPTGAGLGGGS
                     ANGAFTLQLLNKLFQLQLTTNQLINFALQLGSDCPFFIINTPCYSTSRGEQLQPINLS
                     LSNYYFVIVNPQIHISTATAFSTIQPIKPQKSVSEIIHQPIETWKEDLVNDFEKNIFL
                     QYPAIEKIKINLYQQGAVYASMSGTGSTVYGIFERESSFVNYFPSNYFVQQVI"
     gene            complement(8480..9064)
                     /locus_tag="PLU36_03170"
     CDS             complement(8480..9064)
                     /locus_tag="PLU36_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HMZ45781.1"
                     /translation="MKKIELEIIALSHSMTQSNSYAVVLGEISGIRRLPIVIGAFEAQ
                     AIAVALESIHPSRPLTHDLMKNFMTAFGVSLQEVIIHDLQEGIFFSKLICYSTNETIE
                     IDSRTSDAIALAVRFGCPIYTTSNVIDSAGIVLDESSTKKKTIENITTDETLSSNDFS
                     NFTLEELQNLLNDVLEQEDYIKAIAIRDEINKRK"
     gene            complement(9150..9458)
                     /locus_tag="PLU36_03175"
     CDS             complement(9150..9458)
                     /locus_tag="PLU36_03175"
                     /inference="COORDINATES: protein motif:HMM:NF024232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HMZ45782.1"
                     /translation="MLKNKLSALRIIGFAEAASWLLLLLIAMPLKYIWMKPLAVKIVG
                     WIHGILFITYITLALLVAKEHKWKFSKLINAFISAFVPLGTYFFDSRLKKEQLEFNKP
                     "
     gene            complement(9503..10519)
                     /locus_tag="PLU36_03180"
     CDS             complement(9503..10519)
                     /locus_tag="PLU36_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45783.1"
                     /translation="MKIFCLILSTCFCIATKAQKLTHKNYNTLQELEKSITTYSKDMI
                     MAEEAIDRYRADSFFTRGLVKALKTTYSFYYNFDSINTISKLYAPDSSFKIFTWQLVK
                     DYSSIRQKGAIQINTPDGSLKLFPLFDASDDMENPVDSTRNTTRWVGALYYKIILTTF
                     NNKKYYTLLGLDENNEKSNKKWIEVVTFNKEGHPVFGGRYFQYPNDELKPAQPAFRFC
                     LEYKKDARARLTYDDEIGAIVFDHLISEEKKPENRNTLIPDGDFEAFRWIDGRWIHVN
                     KLFDYSVDMTGVDPTIGNPPVPKPIKDKKPSKKEQKEIEKFDPYKVNEKRNKKEDQRN
                     KEEY"
     gene            10710..11465
                     /locus_tag="PLU36_03185"
     CDS             10710..11465
                     /locus_tag="PLU36_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45784.1"
                     /translation="MKNWFLFLFLMCVTILNSKAQTSFIEYQKKFPRMVNALKTKEDT
                     LKKQFKEQNLQWPPKQLYIRSFKYDSQLEVWVRSNNKENYTLFKTYKVCALSGNLGPK
                     RIQGDYQVPEGFYYITDFNPKSEYHMSLGLNYPNASDLLLSDSAKPGSEIYIHGKCIT
                     VGCIPIMDKPMEELYILSSYAKHSGEDFIPVHIFPIKYTNYKSAQKLKVVTEEDKEYQ
                     IFTTKLKEVFDYFELHKQLPIISVNKKGEYVIM"
     gene            11517..12032
                     /locus_tag="PLU36_03190"
     CDS             11517..12032
                     /locus_tag="PLU36_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45785.1"
                     /translation="MKKGIKILLSTLAITLFTLSVNAQTEWVSQKIGNKIIAKFPTAP
                     IEVQAGAVYSLTDADSTNFTATFVDLEAMGLDSATLAGLVETEEFSEQFKTGLTSQMP
                     GVEVTKSDITKWNDLVAYNIEGTNASKKEKVTFKCIFWGSKMFTFICTYRDSVGPKNK
                     DIYINSLALIK"
     gene            complement(12100..12702)
                     /gene="infC"
                     /locus_tag="PLU36_03195"
     CDS             complement(12100..12702)
                     /gene="infC"
                     /locus_tag="PLU36_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HMZ45786.1"
                     /translation="MALPFKANSGGRGKFNPRFNRNQEPEHRINERIRVPQVRLVGDN
                     VEVGIYSTQEALRIAQDLELDLVEISPQADPPVCKAIDYKKFLYEKKKKEKEMKANAK
                     QSEVKEVRFTPGTDDHDFDFKAKHAENFLKEGNKVKAYVQFKGRAIMFKERGELVLLK
                     FAERLTEFGQPETLPKLEGKRMFLMLTPKTNKKKKEEKPS"
BASE COUNT         4468 a         1975 c         1891 g         4526 t
ORIGIN      
        1 aactcacgac ctcttcctta ccaaggaagt gctctaccac tgagctacaa cggctggcag
       61 agtttagaag tgagctaact actctcacaa ttcattgcat tttaaagaac taatttttat
      121 ttgagcggaa gaccgggctc gaaccggcca cctgaagctt ggaaggctac cgctctacca
      181 aatgagctac ttccgctaat tattatttta aatgttgaat tttaaattct aagtaaaaat
      241 tgtaactcaa aatttatcat ttaaaattca aaataatcca aagaactgtg ggcagagaag
      301 gattcgaacc tccgtactcc gaagagagca gatttacagt ctgccgcctt tagccactcg
      361 gccatctacc catacttttc atttaaaatg aaatttaata gagccacttg tcggactcga
      421 accaacgacc tgctgattac aaatcagctg ctctaccaac tgagctaaag tggcttctaa
      481 ttatatacac aaagaactaa cccctttttc gggacggcaa aggtaatgga aaacttatat
      541 ctacaaaatt cttgaaaaga ttttttttta cttcttacaa aattgtgaaa aaaaattata
      601 cacttaatta aaaattgctg attaataaca gcatacaacc tttacaaaca ttgagttgta
      661 taaattaaat cataaaatta cacccaattg atttaagtat aaaaaaataa aaaaaatcaa
      721 aataaaatat tgaaaaaatc aagcaaacta ccctctaatt aataaaaata aattcatacc
      781 caattaaatt ctacaccatt attaagtttt taaaacctaa actacaacgc agttttagta
      841 attttgataa ttatattact taaaaatata ttatgactat agaagatgct caaaacacag
      901 taaacaactg gattaacaca gttggggtta ggtattttaa cgaattaacc aacttaggca
      961 ttttaatgga agaagttggc gaactatcta gaataatggt gagaacctac ggtgagcaat
     1021 cgtttaaaga aagtgataaa gaaaaagaat tagccgatga aatggcggat attttatggg
     1081 ttttaatttg cttagctaac caaaccggta ttaacttaac cgaagcattt caaaaaaatt
     1141 tagataaaaa aaataataga gattttctta gacatcagaa caataaaaag ataaacccat
     1201 cgttagaata aggtagaaaa accttgattc tatgactagc ccccttttta ttacacaaac
     1261 tattattatt tatggttcag caattctatt aattgtagta gcaaccatct taattaaata
     1321 tttttatgca aactctgtta aatgtaaact acaagagtat caaagagaga ttgctaaaag
     1381 tcattcaaga attttgaagt tagaagtaac gaacgatcaa ttaaagcaaa aagtttctga
     1441 gttagaaacc atcattcacc gtcctaaaat agcttaaata ccaacccagt tttaattgtt
     1501 tcattaataa attattctgt ttttcttcta ccaacttacc caagtgtttt ttatattatt
     1561 ttttaccaca cttagattgc aaattgtgtc ggctttttta ttaggtttgc cgccaatatg
     1621 agtattcaac ataacaataa gtttcaagaa attatttccc atgctaaaga gtatggcttt
     1681 gtttttccat ctagcgaaat atatgatggc ttaagtgcag tgtatgacta tggcccttat
     1741 ggtgctgaac ttaaaaaaaa tatacgtgat tactggtgga aaagtatgac acagttcaat
     1801 gacaatattg taggaattga tgctgctatt tttatgcacc ccacaacgtg gaaagcaagt
     1861 gggcatgtag ataattttag cgacccaatg attgataata aagacagcaa aaagcgttat
     1921 agggtggacc atttaattga agcaaaggca gaagaacttg ctttggaagg taaaaaagaa
     1981 gaagagtatg agctattaaa gaaaatggat gaatatttgg ctgccgatga ctttgttgca
     2041 ttaaaaaata ttattgagga atacaaaata aaatgtagtg ttagtggtac aagtaattgg
     2101 acagatatta ggcaatttaa tttaatgttt aaaactgaat ttggtgctgt agctgcagac
     2161 aatgcagaag ataatattgt ttatttaaga ccagaaactg cacagggtat atttgttaac
     2221 tttttaaacg ttcaaaaaac agccagaatg aaaatacctt ttggtattgc acaaactggt
     2281 aaagcattta gaaatgaaat tgttgcaagg cagttcatat ttagaatgag agaatttgaa
     2341 caaatggaaa tgcaattttt tgttcgacct ggtacagaag atgaatggta taaatactgg
     2401 aaaaacgaac gattaaactg gcatttaagt cttggtttgc caaaagaaaa atatcgttac
     2461 cacgaccatg taaaattagc acactatgct aaagaagctt gcgatattga atacgaattt
     2521 cctattggat ttaaagaagt agagggcata cactccagaa gcgattttga tttatctcaa
     2581 caccagcaat tcagtaaaaa gaaacaacaa tattttgaca atgacattaa ccccgaaaca
     2641 ggaaaagctt ttggcaatta tattccttat gtaattgaaa catctattgg cttagacaga
     2701 atgtttttgc ttgtactttc taatgcttac gaagaagaaa ccataactaa agaagatggt
     2761 agtacagaca ctagaattgt acttcacatt cctgcaaaac ttgcacctgt aaaattagct
     2821 gtacttccgt tattgaaaaa agatggacta cccgaaattg cacaacagtt aatggcagat
     2881 tgtaaacctt atttcaaatg tttttacgaa gaaaaagatg ctattggtaa acgttacaga
     2941 aggcaagatg ctattggtac accattttgt gtaaccattg accatcaaac aaaagaagat
     3001 aatactgtaa ctattcgttt tagagatagc atgaaacagg aacgtattgc attcaataaa
     3061 gtaagagaat tggtaatgca aaatttgtaa tataataagt tgcctctaaa atgttttaga
     3121 actgaaccct agtttattat aatttatata ggaaagtaat ataattattt ttatattaat
     3181 tttttaaaaa caattcttat tactggttct tacttttctt aaaaaatgaa ataaataaat
     3241 ttggttacta ttttttttgt gtagttttat attttcatta attccctaaa acatgaaaaa
     3301 gatattcaaa tttatagggt atttatttgc aattataata gtactacttg caacagcagt
     3361 tgcttatata aaattggcac tgcctaatgt tggaaaagct acagatttaa aagttgaatt
     3421 aacacaagct aaaatagata gagggcaata cctggctaat catgtaatgg tttgtataga
     3481 ctgccactca acaagagatt ggagcttatt ttcaggacca attgtggcag agacagaggg
     3541 taaaggcggc gaaatttttg atgaaaaatt gggctttcct ggaaaatatg tttctcctaa
     3601 ccttacgcct tacaaattaa aaagttggac tgatggagaa atttttagag ctataacaag
     3661 tggtgttacc aacaaaggca aagcattatt ttctttaatg ccttatcata actatggcaa
     3721 actagataaa aatgatattg aagctgtaat agcatattta agaacattaa agccaattga
     3781 gagtacacag gaaagttcta catccaattt tccagtgaat attcttatta atacttttcc
     3841 ttcaaaacca aattttacca ccataccaaa tacaacaaat actattgaat atggcaaata
     3901 tcttactact gctgcaggtt gctatgattg ccataccaaa caagagaaag gaaaatttgt
     3961 tggagatgat tatgcaggag gctttgaatt taagtttgcc gatgggacaa ttgtgaggtc
     4021 ttccaatatc acatcagata aagcaacagg gttaggaagt tggactgtag aagcatttgt
     4081 aaacaagttt aaagtttatg aagattctac ttttgaatta ccaaaagtaa acccaggtaa
     4141 tttccaaact gtaatgcctt ggacgatgta tgcaggaatg aaaaaagaag acttagctgc
     4201 tatttttgcc tatttacaaa cagtaaaagc tgtaaacaat actccggaga aatttacttc
     4261 tgctaaatta attaagaact aacagttgta ctgcgttttt tatgctttct cttctttttg
     4321 ggtttataat ataagaagta gagtagcaat aaaaaataga taattgctgc agctaaaaat
     4381 gcaagggtta attttttatt acccaaatct aggtaaaaaa aaccaacaac agcaaatact
     4441 atactttcta taagaaaaac aatttttacg ctattgccaa atattttttc tagattttct
     4501 atggaagctg acactaaaag aaacagaatg gagaaaagca acaatacaat acaaaagcta
     4561 aactttttgt actcaaacat tttgaaagca attggcaaca gtatgcttcc agcaataaaa
     4621 tgacagatta ttttaaatct atcgttcttc attaattgta ttttgcttta tattttaaaa
     4681 tgcttaccca tacaactcta aatgaatatc ctttttatca tatcctaatg caataattct
     4741 ttctttagcc tcatcaatca tatttttcca accacacaaa taaaaacgag ctggttgttt
     4801 attttgttgt agcaagtttt cataaacacc atgtacatac cctttatatt ctccttgttt
     4861 aatattctcc tctcttgaaa aaacaggatg gtaataaaag tgaggtaatt tttcttgcaa
     4921 ggcttctaac tctgctctat ataaaacatc tgcatatttt ctgcatccat aaataatatg
     4981 caggttttgg tgcggtatat ttttttgaaa aatattattt accattgacc taaaaggagc
     5041 aatgcctgtg cctgtgcaaa taagaaaaat atctttatca atattctctg gtagtacaaa
     5101 tttgccttgt gggccacgca aagtaaatgt tgagccaact tctatattat tgaaaatata
     5161 agttgtacct agacctcctt ctaacaatac aataactaat tcaattacgt tggtaccatc
     5221 tggagaagat gcaatggagt aactacgcca acgcttattt ttttgttcgt gaatgggtaa
     5281 atctaaagtt acaaactgtc ctgctgtaaa ttcaaagtta gatagctctt caatttctat
     5341 ccaatacctt cttgtattag cagtttcttg ttctatcttt attaccttac cgattctcca
     5401 tggttcaagc atggttatat attttgacac aatttatgtt acaaattgtt tttatacaac
     5461 tattttattt ttattttata aaactatttt ttggcaataa ataaaaagac tcctatatta
     5521 tagtttcttt taaattttat gaaaatgaaa aacttattta tcatacgaca cgccaaaagc
     5581 agtactaatt ttttaaataa taacgatttt gaaagaccat taaatgaaca aggaataaaa
     5641 gatgccgctt tgatggctaa aaaactttta agtaagggaa ttattataga tggaattgtt
     5701 actagtactg caattagagc tgctactaca gcaaactatt ttgcaactgc ttttgcaatt
     5761 aataaaaata atttttggaa tgaaccaacg ctttataatg ccaataaaaa aactttttac
     5821 accattatca ataattggca atttacatta aacaatatag ctattttttc tcacaaccca
     5881 ggtataactc attttgcaaa tagtgttacc aattcaaaaa ttgacaatat gccgccttgt
     5941 ggtgtttttg ctataaaaat taattgcaat aagtggcaaa gttttgaaac ctcagaaaaa
     6001 aaattttggt ttttcgattg cccggaaaaa atatggtaac aaatttttat tgagaataca
     6061 tttcaaataa ttttatacac tctatagctt cttttacatc atgcactctt aaaatattgg
     6121 ctccatttag taaacaaatt gtatgtaaag cagtggtacc attaagtgct tgctctggtg
     6181 taactcctaa tgttttataa atggttgatt ttcttgaaac gcctatcaat attggtttct
     6241 ctaaaataga aaaaacagac aactctttta gtaactgaaa attatgttga attgttttac
     6301 caaatccaaa gcctacatct ataattacat cttttattcc cgcttgttta gcttgattta
     6361 ttttatctat aaagaaagat aaaacttctt gtgttacatt atcgtaaata ggattttgtt
     6421 gcatggtttg tggtgtacct tttatatgca tacacacata aggcacattc atattagcca
     6481 cagtagccat catattttta tcaaatgtgc caccacttat atcattaaca atagatgctc
     6541 ctgcctctac tgccttgctt gcaactgttg cattataagt gtctattgat atgattaagt
     6601 tgctaaattt ttttttaatt gcttctatta catttactgt tcttctcaat tcttcctcta
     6661 ctgtaattgg aaggctattt ggtttggtag attggccacc aatatcaatt attatagctc
     6721 cctcttgtac catttgctct accttaaaca aagcagaatc tattgagttt tgttggctat
     6781 tggtataaaa agaattagga gaagtattaa taatacccat tactataggt ttagttatag
     6841 taagcaattc tcccttacaa tttaatgtaa acattcaaaa aaaattttat taaagatagg
     6901 ggcaaataaa aaaaacttct ctagtaaata aagaagtttt tgtgcccaga acaggaatcg
     6961 aacctgcaca tcattgctga taccagattt tgagtctgac gcgtctacca gttccgccat
     7021 ctgggcattt gatgggttgc aaatataata gcatcaatgc aaacctgcca aaattcggtt
     7081 aaggtatttt ttcttgtaat aaaaatcttt tatttgcaac aactcttctc tcgaataaat
     7141 aatttcttta caattttcca aaaggctttt aacaggttca taaatgcttg ttttaaaatt
     7201 attaatttca ttaaccacaa ggtttacttt actttcatcc tgttctaatt ttgcatctaa
     7261 taattgctca ttcaaatcca ttacttgcat taaaaaatta ggtggtagtt tgtatttctc
     7321 ttcatcttct attaaattgt ttaattctaa tatatacttc aatactgact cttgattgtt
     7381 taaaatttta tacgctttat taattaaggc agattgttct aatgcatatt gttgttcatc
     7441 ttgtgttgct tgggtaaaaa aatctggatg aaatttccta ctcaatgcat aaaacttatt
     7501 ttttacatca gataaattgg gattaaagga aattggtata ttaaataatt caaaataatt
     7561 catacataaa tttatatcag caaagttaaa cttacaaggt tgttttaatt tttgatttgc
     7621 aaaatttata ttacttgctg tacaaaatag ttggaaggga aataatttac aaaactagat
     7681 tccctttcga aaataccata tacagtgctt cctgtaccac tcatacttgc ataaactgca
     7741 ccttgttggt ataagttaat tttaattttc tcaattgcag ggtactgcaa aaaaatattt
     7801 ttttcaaagt catttactaa atcttctttc caggtttcta ttggttgatg aataatttct
     7861 gaaacagatt tttgaggttt tataggttgt atagtagaaa atgctgttgc tgtactaata
     7921 tgaatttgag gattaacaat tacaaaatag tagttactta agcttaaatt aattggttgc
     7981 aactgctctc ctcttgaagt tgaatagcaa ggtgtattta taataaaaaa agggcaatcg
     8041 ctacctaatt gtaaagcaaa atttattaat tggttagtag ttaattgcag ttgaaataac
     8101 ttattcagta attgcaaagt aaaggcacca ttggcactgc caccacctaa gcctgcacct
     8161 gttggtatgg ttttatgtaa gtgtagctgt atattgggta gttgtggaaa gtttaatttt
     8221 aataactgcc atgcttttga gcaaatattt tgagatgaat ctacattaat tggtatgcca
     8281 gttattgtaa agttattttc ttttttgtga gtaggtaaaa tttctaaaca atctttaatc
     8341 attaatggat aaaaaattgt ttccaggtta tgaaacccat cttctctttt actaattata
     8401 tttaagccta aattaatttt gcaatttgga aaaactatca aaatgatact tattaatgat
     8461 taggtaacta aatagttttt tattttcttt tatttatttc atctcttata gcaatagctt
     8521 taatataatc ttcttgttct aaaacatcgt tcaataaatt ttgtaattct tctaaagtaa
     8581 aattgctaaa atcattacta cttaatgttt catcagttgt aatattttct attgttttct
     8641 ttttagtgct gctttcatct aaaactatac cagcactatc tataacatta ctagttgtat
     8701 aaatggggca cccaaatctt acagctaatg ctattgcatc gcttgttcta ctatcaattt
     8761 caattgtttc attggttgaa taacaaatta atttagaaaa aaatattcct tcttgcaaat
     8821 catgaataat tacttcttga agcgatactc caaaagcagt cataaaattt ttcattaaat
     8881 cgtgagtgag aggcctgcta gggtgaatac tttctaaagc aactgcaatt gcctgagctt
     8941 cgaatgcacc aattacaatt ggcaaacggc gtattccact aatttctcct aatactactg
     9001 cataagagtt agattgggtc atactatggc ttaatgcaat aatttctaat tcaattttct
     9061 tcataaataa aaactgtttg cccttgcttg gtaaaaaaat tgtagtctca acaaaattaa
     9121 ggtgttctac tatttaacca atgtagtatt taaggcttat taaattcaag ctgttctttt
     9181 tttagtcttg aatcaaaaaa gtaagtaccc aagggcacaa aagctgaaat aaaagcattt
     9241 attaatttgc taaatttcca tttatgttct ttggcaacta atagtgccaa agtaatgtac
     9301 gttataaaca aaattccatg tatccaacca acaattttta ctgccaaagg cttcatccaa
     9361 atatatttta atggcattgc aataagtaat aataataacc aagatgctgc ctcggcaaag
     9421 ccaattattc ttaaagcaga gagcttattt tttagcataa gatgttagat tttttgttta
     9481 aaatgtttac ttaagtgtag tattagtatt cttctttgtt acgttggtct tcttttttat
     9541 ttctcttttc gtttacttta tacggatcaa atttctcaat ttctttttgc tcctttttag
     9601 aaggtttttt atctttaatt ggcttaggca caggaggatt gccaatggta ggatcaacac
     9661 ctgtcatatc tacactataa tcaaataatt tgtttacatg tatccatctt ccatctatcc
     9721 atctaaatgc ttcaaaatct ccatcgggta taagcgtatt tctattttct ggtttttttt
     9781 cttcgctaat taaatggtca aaaacaatgg caccaatttc atcatcataa gttaatcttg
     9841 ctcttgcatc ctttttgtat tctaaacaaa atctaaaagc aggttgtgca ggttttaatt
     9901 catcgttagg atattgaaaa tatctgccac caaaaacagg gtgcccttct ttattaaatg
     9961 ttacaacttc aatccatttt ttattacttt tttcattatt ctcatctaag cctaataaag
    10021 tataatattt cttattatta aatgtggtaa gaataatttt atagtataaa gctcccaccc
    10081 atcttgttgt atttctggtg gaatctaccg ggttttccat atcatcggat gcatcaaata
    10141 atggaaataa ttttaaagag ccatcgggtg tatttatttg tattgcacct ttttgacgaa
    10201 tggagctata atcttttact aattgccaag taaatatttt aaagctacta tctggtgcat
    10261 atagttttga aatggtatta atagaatcga aattataata aaatgaatag gttgttttta
    10321 atgcttttac caaccctctt gtaaaaaaac tatctgccct atatctgtct atagcttcct
    10381 cagccataat catatctttt gaataagttg taatactttt ttctaattct tgtaatgtat
    10441 tataattttt atgggtaagc ttttgtgcct tggtagcaat acaaaaacaa gtactaagaa
    10501 ttaaacaaaa aattttcatg aagttaaaaa aatagaatac taaattagtt tattgttttt
    10561 actttactag taataaaaat taaatgttta aactttaagt tttctttatc ataacccgag
    10621 atttgaaatg aaagttgaaa gctaattttt aaatgttaat ttacacctct atatatcttt
    10681 taattacttt ataaagtatt tttgagccca tgaaaaattg gtttttattt ttgtttttaa
    10741 tgtgtgtaac tattcttaac agtaaagcac aaacctcttt tatagagtac caaaagaagt
    10801 ttcctagaat ggttaatgct ttaaaaacca aagaagatac tcttaaaaaa caatttaaag
    10861 aacaaaactt acaatggccg cctaaacaat tatatataag aagttttaag tatgactctc
    10921 aattagaagt atgggtaagg agtaataata aagaaaacta cacacttttt aaaacctata
    10981 aggtttgtgc attaagcgga aatttaggcc ctaaaagaat tcaaggcgat tatcaagtgc
    11041 ctgaaggttt ttattacatt acagatttta accctaagag tgaatatcat atgagtttgg
    11101 gcttgaatta tcctaatgca tctgatttat tacttagcga ttctgccaaa cctggaagtg
    11161 aaatttacat tcatggaaag tgtattactg taggctgtat tccaataatg gataagccaa
    11221 tggaagaatt atacatactc agttcttatg caaagcatag tggcgaagat tttattcctg
    11281 ttcatatttt tcctataaaa tatactaact acaaaagtgc tcaaaaatta aaagtagtta
    11341 cagaagagga taaggaatat caaattttta ctactaaact gaaagaagta tttgattatt
    11401 ttgaactaca taagcaatta ccaattatat ctgtaaataa aaaaggtgaa tatgttatta
    11461 tgtaatgctt attacattaa tattgtgcca ttattcaatt caaattttta aaaaacatga
    11521 aaaaggggat taaaatattg ttaagcacat tagctataac attatttaca ttaagtgtaa
    11581 acgcacaaac tgaatgggta tctcaaaaaa ttggcaacaa aataattgca aaatttccta
    11641 cagcaccaat agaggtacag gctggtgctg tatatagttt aacagatgct gactctacca
    11701 atttcactgc tacatttgta gatttggaag caatgggttt agactctgca acacttgcag
    11761 gattggtaga aacagaagaa ttctctgagc agtttaaaac tggtttaact agtcaaatgc
    11821 caggtgtaga agttacaaaa tctgatatta caaaatggaa tgacttagta gcttataata
    11881 ttgaaggcac taatgcttct aaaaaagaaa aggtaacttt taaatgtatt ttttggggat
    11941 caaaaatgtt tacatttatc tgcacctatc gtgatagtgt agggccaaaa aataaagata
    12001 tatatataaa ctctttagcg ttgattaaat agtttttata gttaatccat aaaaaaacct
    12061 ctccaaaagg agaggttttt cttttttatt taacccttat taacttggtt tttcttcttt
    12121 cttcttttta tttgtttttg gtgtaagcat taaaaacatt cttttacctt ctaattttgg
    12181 taaagtttct ggctgaccaa attcggttaa tctttcagca aattttaata atactagttc
    12241 gcctctttct ttaaacataa tagcacgtcc tttaaactgt acataagcct ttaccttgtt
    12301 tccttctttt aaaaaatttt ctgcatgttt tgctttaaaa tcaaaatcat gatcgtctgt
    12361 acctggtgta aaacgtactt cttttacttc actttgctta gcattagcct tcatttcttt
    12421 ttccttcttc tttttttcat aaagaaattt tttgtagtct attgctttac aaacaggagg
    12481 atcagcctga ggtgaaattt caaccaaatc taattctaaa tcctgagcaa ttcttaatgc
    12541 ctcttgtgta gaataaatac caacttctac attatctcct actaaacgaa cttgaggtac
    12601 acgtattctt tcattaatac gatgttctgg ttcttgattt ctattaaaac gtgggttgaa
    12661 tttacctctg cctccagagt ttgctttaaa tggtaatgcc attaaaattt atttggttaa
    12721 aaattgctgg tttaaaatta attaatattt gattaattta atgattttct atttattatt
    12781 tcttctacaa tgcttttagt aaactcttct attgttagtt ggcctacatc tccttttcct
    12841 tgcctacgaa cagagagttt
//
LOCUS       DAOQPK010000038        12858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_2506_length_12858_cov_4.487542, whole genome shotgun sequence.
ACCESSION   DAOQPK010000038 DAOQPK010000000
VERSION     DAOQPK010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12858
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2506_length_12858_cov_4.487542"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..1083
                     /locus_tag="PLU36_03200"
     CDS             118..1083
                     /locus_tag="PLU36_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HMZ45787.1"
                     /translation="MSVLIIIDKADGQIKKSSLEALTYGADVAKQLNTTAAALVLGTC
                     TDDLSTLGKYGVGKVYQVVEDSLNNLDAQVYAQVIASVTNQVGANVLIFSHNQTGKSV
                     AARVAANLKAGIVTGACALPDVSNGFVVKKTVFSGKAFANVNINSACKVISLNPNSYK
                     TVATEGSAIIETLNVSVPTAKVKVTGINKVSGEVPLTEAEIVVSGGRGLKGPENWGII
                     LDLAHVLGAATACSRPVGDAHWRPHHEHVGQTGIQIAPNLYIAVGISGAIQHLAGVNR
                     SKVIVVINKDAEAPFFKAADYGIVGDAFEVLPKLTAEIKKLKGIA"
     gene            1242..2348
                     /locus_tag="PLU36_03205"
     CDS             1242..2348
                     /locus_tag="PLU36_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HMZ45788.1"
                     /translation="MKLVTKFIFTFSFLFFAWKSHAQFRKYSNEFLNIGAGARSLGMG
                     NVQVATANDATAGYWNPSGLIHIKKSPSLSVMHANYFAGIGKYDFAAIALPSKSSKRT
                     LAITFLRFAVDDIPNTLYLIEPDGSPNYNNISSFSSADYAVLLSYSQIAKQKENLFIS
                     YGGNVKIIHRKVGSFAKAWGFGIDAGIQIKKNKWLFGATIRDVTTTFNAWSFSFTEKE
                     KEVLYLTNNDIPVKSTEMTAPRLIAAAARNFKINKNINLLAEANLHFTFDGKRNTLIS
                     TNPISIDPNIGLEASLNNTIFLRAGVSNFQRALKDADTTNQKKVWIFQPSLGAGFKFG
                     NVGIDYAFTNLANQSNPLYTHIFCLRIDLVKKEK"
     gene            complement(2359..3630)
                     /locus_tag="PLU36_03210"
     CDS             complement(2359..3630)
                     /locus_tag="PLU36_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HMZ45789.1"
                     /translation="MNNFTPLAERLRPYELTDLIGQEHLTGNNSILRKAIEQGKIPSI
                     ILWGPPGVGKTTIANIIANKLHQPYYQLSAISSGVKDVREVIEKATAQKHAILFIDEI
                     HRFNKNQQDALLAAVEKGIITLIGATTENPSFEVNSALLSRAQVYVLKHLTEEDLMQL
                     LHIAIKKDVFLKEKNITLQETEALINLSSGDARKLLNLLELVVNSITTTNQQITITNK
                     QVIEIAQQKIALYDKQGEQHYDIVSAFIKSMRGSDPNGAIYWLARMIDAGEDIKFIAR
                     RMLVFASEDIGNANPNALLLANTTFDAVNKIGYPEGRIILSHCATYLASSVKSNASYV
                     AIDKALQTVQQQGNLPVPLHLRNAPTKLMKDLEYGKNYQYSHNGEGNFIEQEYLPDAL
                     KGTVFYSPGNNSRENELRNYLREKWKKKYNY"
     gene            complement(3724..6171)
                     /locus_tag="PLU36_03215"
     CDS             complement(3724..6171)
                     /locus_tag="PLU36_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ45790.1"
                     /translation="MKQIITLLSCIFFSAVTFAQQIKGNVKDEKSNGINAASVLLHNS
                     KDKSITKILVTNAEGAFKFMDIPKGEYYVEINSLGYVAKQSAVFLVDGNDVVLTDFIL
                     EKKSKQLDGVTVTSKKPPVEVKADKTIVNIEGSINAQGSDAFELVRKSPGVTIDKDDN
                     ITLSGKNGVVIYIDGKPSPLKGKDLADFLRTMNSNQIEAIELITNPSAKYDAAGNAGI
                     INIKLKKNKTFGTNGSFNAGYGIGVYGKYNTSLSLNHRNNKVNIFGTYSFNNSKNENN
                     MHFKRYLLDTLFNQSNVMTSSSQTNNIKLGMDYFINKQQTIGFIATGNFNNNDLNHNS
                     NTPITYIPTNTGNYILEAKNTNKGKRNNVTLNLNYKYTNAKGKELNVDADYGNYDNFN
                     NQLQPNYYYDFSGTNELSRKIYNIISPSKINLYTVKADYEQPLAKGKLSIGTKTSFVE
                     TKNNFERYDVFTNNKVLDTARSNNFNFKENINALYANYTKQIKNINVQIGLRVENTNA
                     TGNSIGYKKKNNNYVTYDSIFNRNYTNLFPSASITFNKNPMSQWSLSYSRRIDRPAYQ
                     DLNPFEFKLDEYTFQKGNTELTPQYTNSFNLTHSYKYKLITTLNYSYVNNVFTQLVDT
                     IEKSKSVLTEKNLASQNKVSINISYPIIYKWYSAFINTNFNYSHYKANLGIGRTIDLD
                     AFTFNIYMQNTFKLGKGYTAELSGWFNSPSIWEGTFKAKSMGNVDIGVQKQILKGKGN
                     LKLSVSDIFFISKWHVTSNFAGQYFDATGHWESRQFKINFNYRFGKSTVKSSRQRTTG
                     TEEENKRAQGGGGGIGQ"
     gene            complement(6319..6528)
                     /locus_tag="PLU36_03220"
     CDS             complement(6319..6528)
                     /locus_tag="PLU36_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ45791.1"
                     /translation="MKNLIKVERAKKNWTQEYLANKIGVSRQSMNAIEAGKFIPSTLL
                     ALKMAKVFGTSVESIFQLEKSDTLL"
     gene            complement(6525..6926)
                     /locus_tag="PLU36_03225"
     CDS             complement(6525..6926)
                     /locus_tag="PLU36_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45792.1"
                     /translation="MKINKKQINKLILFASILWAACYLIALNVIKEYTLSNEIKVLLS
                     ILPTITFGILIYFYITSINLMDEVEKRIHLEAAVFAFSTGLALIMTIGIVDTAFDLNK
                     EIFGTKKIFIYLFILYFIGVALSKNKYLFKK"
     gene            7102..7965
                     /locus_tag="PLU36_03230"
     CDS             7102..7965
                     /locus_tag="PLU36_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HMZ45793.1"
                     /translation="MEKSIKPIQGFLALFVSLILLAAGVVIIGLYHEKVLLVIVGVIL
                     IITSFFILKGLMIINPNHSRVLNFFGKYVGSVKENGLFFINPFYSSLKVSLRAENLQG
                     QSLKVNDKMGNPIEIAAVIVWKVGDTYKATYEVENYNEYVSKQSEAAVRHLAVSFPYD
                     NLEDEKASITLREGGDKVNQILENELTERLAPAGIIILEARISHLAYAPEIAGAMLQR
                     QQATAIVAARTKIVEGAVGMVDLALKKLSEENIVELDDEKKAAMVSNLMVVLCGEKAA
                     TPILNTGTLYQ"
     gene            7979..8179
                     /locus_tag="PLU36_03235"
     CDS             7979..8179
                     /locus_tag="PLU36_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA binding domain-containing
                     protein"
                     /protein_id="HMZ45794.1"
                     /translation="MAEKKNFILRVDPETFSILEKWASDEFRSVNGQIEYLLNSAIIH
                     SGRRQLHTKKLAGNANTKIKKK"
     gene            8589..9056
                     /gene="mraZ"
                     /locus_tag="PLU36_03240"
     CDS             8589..9056
                     /gene="mraZ"
                     /locus_tag="PLU36_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HMZ45795.1"
                     /translation="MLNLLGEYEATIDSKGRFLIPGGLKKQLPDGESRLVISRGIEKC
                     LTLYPIKSWEPIVARIRLLNDFDPKVREFKRLFLGGATEVELDSAGRLLLPQQLKEYA
                     DLSKDIVLAADLDKINIWDASKYKQFFEDFSPEVFSNLAATVMANKSTNDIGN"
     gene            9081..10022
                     /gene="rsmH"
                     /locus_tag="PLU36_03245"
     CDS             9081..10022
                     /gene="rsmH"
                     /locus_tag="PLU36_03245"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HMZ45796.1"
                     /translation="MMSKQIENTQHPNSNYHIPVLFNETIDTLNINPNGIYVDCTYGG
                     GGHSKGILKNLSSKGKLFAFDQDVDAQENLQSDDRLIFVPHNFRYLQKFLRLYNVQQV
                     DGVLADLGVSSHQFDVGERGFSTRFDGPLDMRMNTNENKTAAKVIQSFTEQQLHKIFE
                     QFGEVTNAKTLAKHIVQNRTKQPLQTIEQFKMLISAVVKGNPNKYLAQVFQALRIEVN
                     EEIDALKEMLLQLNNLVKPGGRAAIITFHSLEDRVVKNFFRNGSFQNELSDNPFAIGE
                     KKKTWKLINHKPIVANEDELKNNKRSRSAKLRVAEKI"
     gene            10029..10406
                     /locus_tag="PLU36_03250"
     CDS             10029..10406
                     /locus_tag="PLU36_03250"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HMZ45797.1"
                     /translation="MAENENIKNTNQPNEKKGLKKGVGVLFNSQWITNNLYFFLFLGL
                     LAIIYIANGHWADKTVRNIDKAKKELRDLQFEYKTIKAELMHKSEEAQVLKAAEPLGL
                     KISKEVPVRLPNDNEPIELKNKK"
     gene            10436..12553
                     /locus_tag="PLU36_03255"
     CDS             10436..12553
                     /locus_tag="PLU36_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HMZ45798.1"
                     /translation="MEVKKDILWRVYLAFIVLVLVCFVIIGKAFYIQQIQGKHWRSMS
                     DSLHQRIEEIDAERGTIFSENGEMLSTSIPQFDIFIDFAAQGLREKNGKRFRENLDSL
                     SFCLSNLFKDKSPAEYKAILSKGFKEKKRYYPLMKNVSYRDYQQLKTFPLVRLGKNKS
                     GFITDDKSIRLNPYKMLAFRTIGLARDSFKVGLEMSYDSLLKGKTGRRLVRSIAGGVT
                     VPVEEYEINAEAGKDIVTTLDVFIQGVTENALMNMMVKNEAANGCAIVMETKTGKIKA
                     IANLGRNATDGSYFEDYNYAINLSEPGSTFKLATLIALLEDKKVNLNSTVNLQGGVWN
                     INGRTVYDSEKHGRNDVTVKQAFELSSNVGMAKLAWSGYGSSPSKFINHLQNLNLDKP
                     TGIDITGERKPIIFKPGSKFWSSTTLPWMSFGYNVSITPLQTLTLYNAVANNGVMLKP
                     YLLNSVQQEGKLLKEFNPTITKEKICSNETLKQLKECLEGVCTEGTAQSLFKNSPYKV
                     AGKTGTALVANGNKGYDDKIYQSSFAGYFPAEDPQYTIIVVIKNKPHALNYYGGTVAG
                     PVFKEISDRLYTTRVKHAPANSVGLQKKDSSTYIFSGNKMDIEQISQKLKLGFQDNSG
                     ITNEWVNITVKNENVAIRNKDINTNRMPALKGLGLKDALTICENKGLKINVKGKGKVI
                     SQSVVEGSIVANGQVINIQLGTL"
     gene            12581..>12858
                     /locus_tag="PLU36_03260"
     CDS             12581..>12858
                     /locus_tag="PLU36_03260"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HMZ45799.1"
                     /translation="MNNLQDILYKVHIKQVIGTTQLNVKNVQIDSRCVSNGSVFIAIK
                     GDKTDGHHFIEQVIQNGAIAVICQTIPERIDPNITYVLVENTHEALAV"
BASE COUNT         4497 a         1780 c         2127 g         4454 t
ORIGIN      
        1 gctgataatg tagctgaatt ggttcgttta cttcacgaag aagcaaaagc aatttaaaat
       61 gtattaaata ttcatttcag aatataattt cttattcaat tattcaaata tataaatatg
      121 tcagttttaa taattataga taaagccgat gggcaaatca aaaaatcttc tttagaagca
      181 ttaacctatg gggcagatgt tgccaaacaa ttaaatacta ctgctgctgc attagtttta
      241 ggtacatgta cagatgattt atcaacttta ggtaagtatg gtgttggtaa agtatatcaa
      301 gtggtagagg atagccttaa taatttagat gcacaggttt atgctcaagt aattgcaagt
      361 gttaccaatc aagttggagc aaacgtttta atcttttctc acaaccaaac aggtaaatca
      421 gtagctgcca gagttgctgc taatcttaaa gcaggcattg taactggtgc ttgtgcatta
      481 ccagatgtaa gcaatggctt tgttgttaaa aaaactgttt ttagtggtaa agcttttgcc
      541 aatgtaaata tcaactctgc ttgtaaagtt atatctctaa atccaaacag ttataaaact
      601 gttgcaacag aaggtagtgc aataatagaa acgttaaatg taagtgtacc aactgcaaaa
      661 gttaaagtaa ctggcataaa taaagttagt ggagaagtgc ctttaactga agcagaaatt
      721 gtggtaagtg gaggaagggg tttaaaaggt ccagaaaatt ggggaattat tttagacctt
      781 gctcatgtat tgggtgctgc aacagcttgt agcaggcctg taggggatgc ccattggaga
      841 ccacaccatg aacatgtagg gcaaacaggt attcaaattg ctccaaactt gtacattgct
      901 gttggtatta gtggtgctat tcaacattta gctggtgtaa acagaagcaa ggtaattgtt
      961 gtaattaaca aagatgcaga agcaccattt tttaaagccg cagattatgg cattgttggc
     1021 gatgcttttg aagtgctacc taagcttact gctgaaatta aaaaactaaa aggtatagca
     1081 taaactacct aataagtata gtattaattg gttattttaa aggaaaaatt ggttattgaa
     1141 ctcttaaatt taatattcaa taatcttctt ttgaaataac caatttttta ttaaagtaat
     1201 tctaatattt ttacagcagt attcaagcac attaaaactt aatgaaactg gtaaccaaat
     1261 ttattttcac tttttctttt ttattttttg catggaagtc tcatgcacaa tttagaaaat
     1321 actctaacga atttttaaat attggagcag gtgcaagaag ccttggaatg ggtaatgtac
     1381 aagtagccac agcaaatgat gcaactgcag gttattggaa cccttcaggt ttaattcata
     1441 tcaaaaaatc tccttcttta tcagttatgc atgcaaacta ttttgcaggt attggtaagt
     1501 acgattttgc agctatagct ttgccatcta aatcaagcaa aagaactttg gcaattactt
     1561 ttttacgttt tgctgtagat gatattccta acacattata tttaatagaa cccgatggat
     1621 caccaaacta taataatatc tcaagttttt catcggcaga ttatgctgtt ttgttaagct
     1681 attcgcaaat tgctaaacaa aaagaaaatt tatttattag ttatggaggt aatgtaaaaa
     1741 ttattcatcg taaagttggc agctttgcta aagcatgggg ttttggaata gatgctggaa
     1801 ttcaaattaa aaaaaataaa tggctttttg gtgcaacaat aagagatgtt actacaactt
     1861 ttaatgcctg gtctttttcg tttacagaaa aagaaaagga agtattatac ttaactaata
     1921 atgatatacc tgtaaaaagt acggaaatga ctgctccaag attaattgct gcagcggctc
     1981 gtaattttaa aatcaataaa aatatcaatt tattagccga agctaatttg cattttactt
     2041 ttgatggtaa aagaaataca ttaattagta ccaaccccat aagtattgac ccaaatattg
     2101 gattagaagc aagtttaaac aataccattt ttttaagggc tggagtttcc aattttcaaa
     2161 gggctttgaa agatgcagat acaaccaatc aaaaaaaagt ttggattttt caaccaagtt
     2221 tgggtgcagg gtttaagttt ggaaatgtag gtattgatta tgcttttacc aatttagcta
     2281 accaaagtaa tcctttatac acacatatat tttgtttgag aatagattta gtaaaaaaag
     2341 aaaagtagtt cttatagact aataattata tttctttttc catttttctc ttaaataatt
     2401 tcttaattcg ttttctctac tattattgcc tgggctataa aatactgttc cttttaaggc
     2461 atctggtaaa tactcttgct caataaaatt tccttcgccg ttatgagaat attgatagtt
     2521 tttcccatat tctaaatctt tcataagctt agtaggggca tttcttaaat gcaaaggcac
     2581 aggtaaattg ccttgctgtt gtactgtttg caaagcttta tctattgcta cataagaggc
     2641 attactttta acagaagatg ctaaataagt agcacaatgg cttagaataa ttcttccttc
     2701 tggatatcca attttattca ctgcatcaaa tgtagtattg gctaataata aagcatttgg
     2761 attggcattg cctatatctt cacttgcaaa aacaagcatt cttcgggcaa taaattttat
     2821 atcttctcca gcatcaatca ttctggctaa ccaataaatt gcaccatttg ggtcgctgcc
     2881 acgcatactt ttaataaagg cagaaacgat atcgtaatgt tgttctcctt gcttatcata
     2941 taaagcaatt ttttgctgag caatttcaat tacttgttta ttggttattg ttatttgctg
     3001 attggttgta gttattgagt taactacaag ctctaataaa tttaataatt ttcttgcatc
     3061 accactactt aagttaatga gtgcttctgt ttcttgcagt gtaatatttt tttctttaag
     3121 aaagacatct ttttttatag caatatgtaa tagttgcatt aagtcttctt ctgttaaatg
     3181 ttttaataca tatacttgtg ccctgctcag taaagcacta tttacttcaa aggagggatt
     3241 ttcggtggtg gcaccaatta atgttattat tcctttttct actgctgcta ataaggcatc
     3301 ttgctggttt ttattaaaac gatgaatttc atcaataaat aaaattgcat gtttttgtgc
     3361 tgttgctttt tctattactt ctcttacatc tttaactcca ctacttatgg cacttaattg
     3421 ataatatggt tgatgtaatt tattagcaat aatatttgca atggttgttt taccaacacc
     3481 tggtggtccc cataaaataa tggatggaat tttaccttgc tcaatagcct ttcttaaaat
     3541 actattattg cctgttaaat gttcttgacc aattaaatct gttaattcat aaggtcttaa
     3601 tctttctgct aatggtgtaa aattattcat agctgaaagg tagaatttat tttagttatt
     3661 aaaaaaaaat gccaccaaca agattgttga tgacattcat taaaactaaa cctgagaaaa
     3721 aatttattgg ccaattccac cgccaccacc ttgtgctcgt ttattttctt cttctgttcc
     3781 tgttgttctt tgccttgagc tttttactgt gctttttcca aaacggtaat taaaatttat
     3841 cttaaactgt ctgctttccc aatggccagt agcgtcaaaa tattggcctg caaaattgct
     3901 ggttacatgc catttagaga taaagaatat atcacttaca ctaagcttta agtttccttt
     3961 tccttttaat atttgctttt gtactccaat atctacatta cccattgatt tagctttaaa
     4021 cgtaccttcc caaatactag gtgagttaaa ccaaccactt aattcagcag tataaccctt
     4081 gccaagttta aatgtatttt gcatgtaaat attaaatgta aaagcatcta aatctatggt
     4141 tctacctata cctaaatttg ctttgtaatg tgagtaattg aaattggtat ttataaatgc
     4201 actataccat ttgtaaataa tgggataact gatattaatg cttactttat tttggcttgc
     4261 taaattcttt tcggtaagta cacttttgct tttttcaatg gtatctacta attgtgtaaa
     4321 cacgttattt acatagctat agtttaaggt tgtaattaac ttatacttat aactatgtgt
     4381 taaattaaaa ctgtttgtat attgaggagt tagctctgta ttgccttttt gaaatgtgta
     4441 ctcatctaac ttaaattcaa atgggtttaa atcttgatat gctggtctat ctattcttct
     4501 actataactt aaactccatt ggctcatagg gtttttattg aaagtaatgc ttgcactagg
     4561 aaacaagttg gtatagtttc tgttaaatat gctatcgtaa gttacatagt tattgttttt
     4621 ctttttataa ccaattgaat ttccagttgc gttagtattt tctactctta aaccaatttg
     4681 tacattaata ttttttattt gctttgtata atttgcataa agggcattaa tattttcttt
     4741 aaaattaaaa ttattacttc ttgcagtatc taatactttg ttgttggtaa aaacatcgta
     4801 tctctcaaaa ttattttttg tttcaacgaa agatgttttt gttcctatac ttaattttcc
     4861 ttttgctaaa ggctgttcat aatctgcttt tacagtatat aaattaattt tggaagggga
     4921 aattatgtta taaatttttc tggaaagttc gttagttcca ctaaagtcat aatagtaatt
     4981 tggttgtaac tgattattga aattatcata attaccataa tctgcatcaa catttaattc
     5041 tttacctttt gcattcgtgt atttgtaatt taaatttaat gtaacattat ttctttttcc
     5101 tttattggta tttttagctt ctagaatata attgcctgtg ttagtaggta tataagtaat
     5161 tggtgtattg ctgttgtgat ttaaatcgtt attattaaaa ttacccgttg caataaatcc
     5221 aatagtttgt tgcttgttta taaaatagtc catacctaat tttatgttat tggtttgact
     5281 agaacttgtc ataacattgg attgattaaa taaagtatct aacagatatc ttttaaagtg
     5341 catattgttt tcattcttac tattattaaa actatatgta ccaaaaatgt ttactttatt
     5401 atttctgtga tttaggctta agcttgtatt gtattttcca taaacaccaa taccatagcc
     5461 tgcattaaaa ctaccattgg ttccaaatgt tttattcttt ttaagtttta tatttattat
     5521 accagcatta cctgcagcat cgtattttgc agatgggtta gtaattaatt ctatggcttc
     5581 tatttggttt gaattcattg tacgtaaaaa atctgccaaa tcttttcctt ttaaaggaga
     5641 gggtttacca tcaatataaa ttacaacacc gtttttaccg cttaaagtaa tattatcatc
     5701 tttgtctatt gttacacctg gtgattttct aactaattca aaggcatctg aaccttgggc
     5761 attaatactt ccttctatgt ttacaattgt tttgtctgcc tttacctcaa caggaggttt
     5821 ttttgaagtt acggttacac catctaattg ttttgatttt ttttctagaa taaaatctgt
     5881 taaaacaaca tcgtttccat caactaaaaa tacagcagat tgtttagcaa catagccaag
     5941 agaatttatt tctacataat actctccttt aggtatatcc ataaacttaa atgcaccttc
     6001 tgcattggtt actaaaattt tagtaataga tttatctttt gagttatgta ataatacgct
     6061 ggcagcattg atgccattgc ttttttcatc ttttacattg ccttttattt gttgggcaaa
     6121 tgtaactgca ctaaaaaaaa tgcaggataa taatgtaatt atttgtttca tataatttga
     6181 tagtttagag tttataattt gaaatcaaaa ctgaaaattc taaactattt tttctattag
     6241 attcttacca atggaaataa aaaacctttg agcggtagaa gctatataga acaatgaaac
     6301 gaggaataat ttaaaaagtt acaaaagcgt atcacttttt tctagttgaa aaatactttc
     6361 aacagaggtt ccaaagacct ttgccatttt aagtgcaaga agtgtagaag gtataaattt
     6421 tccggcttct attgcattca ttgattgcct tgaaacacct attttattgg ctaaatactc
     6481 ttgtgtccag ttcttttttg ctcgttctac tttaattaaa tttttcattt tttaaacaag
     6541 tatttgtttt tagataaggc aacaccaata aaatataaaa taaataaata gataaatatt
     6601 tttttggttc caaatatttc tttgtttaaa tcaaatgctg tatctacaat accaatggtc
     6661 attattaatg ctaaacctgt agaaaatgca aatactgcag cttctaaatg aattcttttt
     6721 tccacttcat ccattaagtt aatgctggtg atataaaagt agattaatat gccaaatgtg
     6781 atagtaggaa gaatggatag taatactttt atttcattgc ttaatgtata ttctttaata
     6841 acatttaatg ctattaaata acatgcagcc cataaaatac ttgcaaataa aatgagcttg
     6901 ttgatttgtt ttttgttaat cttcataatg taaatgattt aatttttcac aaagataagt
     6961 attttttaca aaatgtaaaa taaactttac aaaatgtata tatttcttgt cataaatttt
     7021 tttttacttt ttcaaataaa atttggctac tctataaaac taatatactt ttgatattat
     7081 aataatatca aaatgaattg tatggaaaaa tctattaaac ctatacaagg ctttttagcc
     7141 ttgtttgtat ccttaatttt actagctgct ggagtagtaa taattggctt gtatcacgaa
     7201 aaagttttac tagttattgt tggtgtaatt ttaataatta cctcgttttt tattctgaaa
     7261 gggctaatga taattaatcc taatcactcc agagttttaa atttttttgg taaatatgtt
     7321 ggtagcgtaa aagagaatgg attgtttttt ataaacccat tttactcctc cttaaaagta
     7381 agtttaagag ccgaaaacct gcaaggacaa tcattaaaag taaatgataa aatgggtaat
     7441 cctattgaaa ttgcagcagt aattgtttgg aaagttggtg atacttacaa agcaacctat
     7501 gaagtagaaa attataacga atatgtaagt aaacaaagtg aggctgcagt aaggcatttg
     7561 gccgtaagtt tcccgtatga taatttagaa gatgaaaagg catctattac tttaagagaa
     7621 ggaggagata aagtaaatca gattttagaa aatgaattaa cagaaagatt agcacctgct
     7681 ggaattataa ttttagaagc aagaattagt catttggcat atgcaccaga aattgcagga
     7741 gctatgctac aacgccaaca agctacagca atagttgcag ctcgtacaaa aattgtagaa
     7801 ggtgccgtag gcatggttga tttggcgtta aaaaaattat cggaagaaaa tattgtagaa
     7861 ttagacgatg aaaagaaagc tgcaatggtg agcaacctta tggtagtttt atgtggcgaa
     7921 aaagcagcca cacctatatt aaatacaggt actctctatc aataataaat attttatttt
     7981 ggcagaaaag aaaaatttta ttttaagggt agatccagaa accttttcta ttcttgaaaa
     8041 atgggcttct gatgaattta gaagtgtaaa tgggcaaata gaatatttgc taaatagtgc
     8101 aattatacat tctggcagaa ggcaacttca cactaagaaa ttggcaggca atgccaatac
     8161 aaagatcaaa aagaaataaa ttatacctct atataaatca tgtagaggta tttttttatt
     8221 caattcatag attttctctt ttacaaaact gttctttaac tactacaata agctatgtat
     8281 taatgttaag aaatgcagta taaaatacat ttttccacaa attgcattag gtgttttggg
     8341 ttttaatatg ctgggtaccg cattttattt agtttttcaa acattcccac accttattaa
     8401 ggcttattca cacttattaa cacttcatta agggtgttat tgctaagaat cttctgaagt
     8461 gctttattta caagcatcct gttaaaatat tatttgtgga taaatacaaa cactataatt
     8521 gtgttttaaa gtgggaaaat gtgttatttt gtgtcaataa atgttaaaaa ttatctcaaa
     8581 ccattaaaat gctaaatctt cttggtgaat atgaagcaac gattgactca aaaggtcgtt
     8641 tccttattcc aggaggctta aaaaaacagc ttccggatgg ggaaagccgt ttagttatta
     8701 gccgtggtat tgagaaatgt ttaaccctat atccaataaa aagttgggag cccattgttg
     8761 cacgtattag attattaaat gattttgacc caaaagtaag agaatttaaa cgcttgtttt
     8821 taggtggtgc tacagaggtt gaattagata gtgcaggaag attattgtta ccccaacaat
     8881 taaaagaata tgccgatctc agcaaagaca ttgttttggc tgcagattta gacaaaataa
     8941 atatctggga tgcaagtaag tacaaacagt tctttgagga tttttcacca gaagtattta
     9001 gcaatttagc tgctactgtt atggccaata aaagtacaaa tgatataggt aattagaatt
     9061 tgggaacaat aaaaaataaa atgatgagta agcaaataga aaacactcaa catccaaatt
     9121 caaactacca tattcctgtt ctttttaatg aaacaattga tacattaaat attaatccaa
     9181 atggaatata tgttgattgt acgtatggag gaggtggcca tagcaaagga atattaaaaa
     9241 atttaagtag taagggtaaa ctttttgcat ttgatcaaga tgtagatgca caagaaaatt
     9301 tgcagagtga tgacaggtta atttttgtgc ctcataattt tcgttatctg caaaagtttt
     9361 taagattgta taatgtgcaa caggtagatg gtgttttagc ggatttgggt gtaagctctc
     9421 atcaatttga tgttggcgaa agaggctttt caacaaggtt tgatggccct ttagatatga
     9481 gaatgaacac taatgaaaat aaaactgctg ctaaagttat tcaatcgttt accgaacagc
     9541 agctgcataa aatatttgaa caatttggcg aggttactaa tgcaaaaact ttggcaaagc
     9601 acattgttca aaacagaact aagcagcctt tacaaacaat agaacagttt aaaatgctaa
     9661 taagtgctgt ggtgaaagga aatcctaata aatacttggc tcaggtgttt caggcattgc
     9721 gaattgaagt aaatgaagaa attgatgcat tgaaagaaat gttgctgcag ttaaacaatt
     9781 tggtaaagcc aggaggcagg gcagcaatta tcaccttcca ttctttagaa gatagagtag
     9841 tgaaaaattt tttcagaaat ggttcatttc aaaatgagct gtcagataat ccttttgcaa
     9901 ttggtgaaaa gaaaaaaact tggaagttaa ttaaccacaa accaattgta gcaaacgaag
     9961 atgaactgaa aaataataaa cgtagcagaa gtgcaaaact tagagtagct gaaaaaatat
    10021 aacatctaat ggcagaaaat gaaaatataa aaaacaccaa tcagccaaac gaaaaaaaag
    10081 ggcttaaaaa gggagttgga gtattgttta actcacaatg gataacgaac aacttatact
    10141 tttttttatt ccttggttta ttggcaatta tttatatagc aaatggacat tgggcggata
    10201 aaaccgtcag aaatattgat aaagctaaaa aagaattaag ggatttacaa tttgagtata
    10261 aaactataaa agcagaattg atgcataaaa gcgaagaggc tcaagtgctt aaagctgcag
    10321 aacctttagg cttaaaaatt agtaaagaag tacctgtaag attaccaaat gataatgaac
    10381 caattgaatt aaaaaataaa aagtaggcat tgaaattatt gattgtttat aaaaaatgga
    10441 agtaaaaaaa gacatactat ggagggttta tttagcattt atagtgctag tattagtatg
    10501 ctttgtaata attgggaaag cattttatat acaacaaatt cagggtaaac attggagaag
    10561 tatgagcgat agcttgcatc agcgaattga agaaatagat gcagaaagag gaaccatatt
    10621 tagcgaaaat ggtgaaatgt taagtaccag cattcctcaa tttgatattt tcattgattt
    10681 tgcagcacag ggtttaagag aaaaaaatgg aaaaagattt cgtgaaaatt tagattcatt
    10741 gagcttttgt ttaagcaatt tatttaaaga taaatcgcca gcagaataca aagcaatttt
    10801 aagtaaagga tttaaagaga agaaaagata ttatccatta atgaaaaatg tttcgtatag
    10861 agactatcaa caattaaaaa cttttccttt agttagatta ggaaaaaata aaagtggatt
    10921 tattacagat gataaaagca ttaggcttaa tccttataaa atgttagcat ttagaacaat
    10981 tggtttagcc agagattctt ttaaagtagg attggaaatg tcttacgata gtttactaaa
    11041 aggcaaaaca ggaagaaggt tagttcgttc aattgctggt ggtgtaacag tacctgtaga
    11101 agaatatgaa ataaatgccg aagctggaaa agacattgtt accacgttag atgtttttat
    11161 acaaggtgta acagaaaatg ctttaatgaa tatgatggtt aagaatgaag cagctaatgg
    11221 ctgtgctatt gtaatggaaa caaaaacagg taaaattaaa gcaattgcta atttaggaag
    11281 aaatgcaaca gatggttctt attttgaaga ttataattat gccattaatc tgtctgagcc
    11341 tggatctact tttaaacttg ctacattaat tgctttactt gaagataaaa aagtaaacct
    11401 aaactctaca gttaacttgc aaggtggtgt ttggaatatc aatggaagaa cggtttacga
    11461 tagcgaaaaa catggaagaa atgatgttac tgttaaacag gcatttgagt taagcagcaa
    11521 tgtaggtatg gcaaaattag catggagtgg ttatggttct tcaccatcaa aatttattaa
    11581 tcatttacaa aatttaaatt tagataagcc tacaggtatt gatattacag gcgaaagaaa
    11641 gccaattatt tttaagcctg gcagcaagtt ttggagttct actactttac cttggatgag
    11701 ttttgggtac aatgtttcca tcacaccact tcaaacatta accttatata atgctgtggc
    11761 taataatgga gtaatgctta aaccttattt gctcaattca gtacagcaag aaggaaaatt
    11821 gttaaaagag tttaatccaa caattacaaa agaaaaaatt tgtagtaacg aaacattaaa
    11881 acaattaaaa gaatgtttag aaggtgtttg tacagaaggt actgcacaaa gtttgtttaa
    11941 aaattctcca tataaagtag caggtaaaac aggtactgct ttagtagcaa atggtaataa
    12001 aggttatgat gacaaaattt atcaaagctc ttttgcaggt tatttccctg cagaggatcc
    12061 acaatatact atcattgttg ttattaagaa taagccacat gctttaaatt attatggtgg
    12121 cacagttgct ggtcctgtat ttaaagaaat atcagataga ctttatacca caagagtaaa
    12181 acatgcacct gctaattcag ttggtttaca aaaaaaagat agtagtacct atattttttc
    12241 aggtaataaa atggatatag aacaaatttc tcaaaaacta aaacttggtt ttcaagataa
    12301 cagtggcatt actaacgaat gggtaaatat tacagttaaa aatgaaaatg ttgcaataag
    12361 aaataaagat ataaatacta acagaatgcc agcattaaag ggtttggggt tgaaagatgc
    12421 actaacaata tgtgaaaaca aaggactaaa aattaatgta aaaggaaaag gaaaagtaat
    12481 tagccagtct gttgttgaag gaagtattgt agcaaatggg caggtaatta atatacaatt
    12541 aggaacacta taaaattatt ttaacaacaa gaataaacac ttgaataact tacaagacat
    12601 attatacaaa gtacacatta aacaagtaat tggtaccacc caactcaatg taaaaaatgt
    12661 acaaatagat tctcgttgtg tttctaatgg cagcgtattt attgcaatta aaggagataa
    12721 aacagatgga catcatttta ttgaacaagt aattcaaaat ggtgctatag cagttatttg
    12781 tcaaacaatt ccagaaagaa ttgatccaaa catcacttat gttttggtag aaaatacaca
    12841 cgaagcatta gctgtatt
//
LOCUS       DAOQPK010000039        12801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_2523_length_12801_cov_4.230582, whole genome shotgun sequence.
ACCESSION   DAOQPK010000039 DAOQPK010000000
VERSION     DAOQPK010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12801
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2523_length_12801_cov_4.230582"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PLU36_03265"
     CDS             <1..411
                     /locus_tag="PLU36_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45800.1"
                     /translation="LLLNCITLLLSFYVAKQKVVKNNIESIHTQDLINHYYLFNYNPY
                     QASLVAQKIAKATKTNLFFQEGFGDSGIKYYLKESNINYSELQESTLLIENKFTLLTN
                     NKKWIEELLVKHHWNLQKKLSNLNYYTVYLCSKN"
     gene            complement(421..1602)
                     /locus_tag="PLU36_03270"
     CDS             complement(421..1602)
                     /locus_tag="PLU36_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45801.1"
                     /translation="MRLNFTIIFNLIFFFSLNSFSNISFAQYSTTNNKNKNNSHTKNI
                     TGVWRGYFIQREFDLLTKKFHEERYKYEVQINNTQTSKLQGVTYSYLDKRFYGKTALQ
                     GIFTVKSNNVLIKESKMLELKMTGNSEPCLMTCYLTYSKIGDKEILKGDYSSTINSSK
                     SDCGEGIVYLEKVPDSDFGKEDFLVNKPNSSLSKSSKNSSPNKKINSAKLKYKPGAED
                     ALVTNNKPIIKKEATPKLVDKIEKKESNQPTAPLEKIEKIEKPITPLPKVLTERKNNL
                     VKTFYVGEGEISLELYDNGQIDNDIVSIYHNGVPVIQNERLSTIPIKLNIKITASEPV
                     QEFILVAESLGEIPPNTSLLKIKAGNKSYQVFLTSDLQKNAKIVFEYKPQNEDSQTPK
                     K"
     gene            1755..3215
                     /locus_tag="PLU36_03275"
     CDS             1755..3215
                     /locus_tag="PLU36_03275"
                     /inference="COORDINATES: protein motif:HMM:NF005548.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMZ45802.1"
                     /translation="MSNKVAIIGAGPAGLSAAYLLGKAGISTTIFEQDKQYVGGISRT
                     ENYKGYYFDIGGHRFFSKSKEVEDFWTEILQDDLLERPRSSRIYYNHTFFAYPLVALE
                     ALKKLGLWQSFLCVASYLKAKLFPVKNPQNVEDWVSNQFGKKLYSIFFKTYTEKVWGI
                     SCKDISADWAAQRIKGLSLSSAIKNALIKPGYASKEKVIKTLIDSFRYPKKGPGMMWE
                     TCRDKCLQLGVKVKMNAEVIGLNFNDNLWQVVMKNEQFDEKFTYVLSSASIKNIVPNI
                     LPSVNKVAKEAALQLKYRDFLTVVLICKDKDSFTDNWIYIHDPSVKVGRIQNFKSWSP
                     YMVPDESMACYGLEYFCFEGDGMWQKNNDDLIDLATNEMHQIGLLNKENVVEGYVVRQ
                     PKAYPVYDKNYKEKVQLVRDGLKPYKGLYLIGRNGMHKYNNQDHSMMTAMFAAKNIIA
                     GKELYDVWKVNQDAEYQEEGERGVNEGGRLIPIVQN"
     gene            3252..3977
                     /locus_tag="PLU36_03280"
     CDS             3252..3977
                     /locus_tag="PLU36_03280"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HMZ45803.1"
                     /translation="MQTTLCFDFGNTRLKCAVFKGAELLQVEVLENDKVANVEVLLKK
                     YQPQKSIFSSVINHNKAIEELLQQKTTFHQLNANSKLPFTTPVGKPETIGADRLAICA
                     GAIHLYPNNHNLAIGLGTCITFNFINKFHEFLGGSISPGMNMRFKAMHQQTALLPLVE
                     ATHNFPLIGYDTKTNLLSGVLLGMAKEIDGIIEAYSKKYNKFNVLITGGDMAFFVSHL
                     KNKIFADPYLIFKGLYAISENNN"
     gene            3952..5319
                     /locus_tag="PLU36_03285"
     CDS             3952..5319
                     /locus_tag="PLU36_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45804.1"
                     /translation="MQLAKTIINRFTATVIFTVCFIFITAAQENSPFSRYGLGDLTNT
                     FQTLNNGMGGVAVAYNDLQSINFNNPASFASTRIVTYDLGVTIDSRILRSKAPVNKYN
                     SANFSPAYIAVATPISSKHSFGLAFGFKPVTRISYNIIRNERLAGIDSVQSLFEGTGG
                     MNEVFVGVGKKWKNLAIGLSTGYHFGKKQNNTKRGFINDTVVYYKANYNTSTTFGGVF
                     LMGGFQYDILLSDSAKNRKITKPIKDFYYLKLGGTFSLGHQLNAEQTSFKETFDYDYS
                     GGSYTIDSLNVGNTIKGKIKIPANFGFGFLFQKTSVDISGVYDRWAIGADLLLSNWKN
                     YRFYDKKDATVNSWQFKIGGQWTPDAKNTKSYFSRVAYRAGLNVGKDYINADGKEMKT
                     FSFSIGLGLPVRSTRFSYQYSTIHTALEIGKRGSAANNITENFFKLSVGFSLSDIWFI
                     KRKYD"
     gene            5312..5887
                     /gene="lptC"
                     /locus_tag="PLU36_03290"
     CDS             5312..5887
                     /gene="lptC"
                     /locus_tag="PLU36_03290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HMZ45805.1"
                     /translation="MISNFKHITFQKAVLVVGCFFVFSCENKIETVRDLGKATLGVEE
                     GKNIESFISQSGKMRAKLNAPIMLRYLLDTPKIEFTKTLFVSFYDDTLGIESNLFAKY
                     GRYLENDNKVYLKDSVVVYNIAGDTLWTSELYWNQDTEEFFTNKPVRVKREFNNKYIH
                     SIGVRANQNLKDITFYKIQPDSYFIVSDSIY"
     gene            6011..10978
                     /locus_tag="PLU36_03295"
     CDS             6011..10978
                     /locus_tag="PLU36_03295"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HMZ45806.1"
                     /translation="MRKMLLLLTCLIVLYSTSIAQYCNSSFSNASFEHITNVTFGGVN
                     NSSTGNTGGPVNYLNKVAYVYYNNSHNLSVSILADASEYVYAFIDWNQDGDFADSDET
                     YTVASNVSTTGPFVLSIATPLTALAGNTRMRVMVDYNGSTPDPCKNSTYGEAEDYTVN
                     VGNMPACMPPTNLAANPVGISTASVSWDASVSNPTGGYQFELRTSGTPGSGIAGLDTT
                     AFTTALTADFTELTISTTYNLYVRAICGAGDSSTWAPYTFSTVYAQARPWIEPFDVLT
                     VPTGWSTTGFTIGTSSSIPDEQTNILYRNLWSGAPNGTFSSVSVGAILAGDALTFNYQ
                     LSDYSSPYGPPAANSCSFVVSISSDFGVTYTDVATVINDGTAGWKTFTQDLAAYIGQY
                     ISVKVTATWLTGDYYLSFDNFAVQSCMPPTNLEIDPLTFTDSTATITWDASISTPALG
                     YKWELRTSGAAGSGPVGLVASDNTNLLTKALTGLTSNTNYTFSILAKCSVTDSSTWAT
                     KMFTTPCSLVSYFNESFDAVTVPALPDCWLKMGAGSAYTQVGTSFSAPNSIYMYSSSG
                     NIAYLRMPPVSGSDLSTHRLRFNAQANGTLGGTIQVGYLTDPNDVSTFVQIGTDFTPT
                     STTSFDNFVLSPVTTITGVNTLVFKHTGTPSYAVFIDNVIYEPIPNCEEPTNVSTSNI
                     TPTTVDLSWDAPTSGTPPLSYSIYYSTVDATPSLTATPNVSGVLGTTATLTNLTAATT
                     YYVFVRSICSATDSSTWSFKKSFTSACVAITSLPWTEGFEGLSTFGAGVVPACWNIEN
                     ISGTKITSANTPIMNGIGPRTGTNYVWSRYGTNNWIFAPAIQLTAGQSYDFSFNYVNV
                     DATSGFTVDVAWGSNPASVDMVNPLGTISNPTNNTYAQAKYSFTPSTTDVYYFGVKVN
                     STFAPWYLCLDDFKVELSPTCNEPTDVTVSNITTNSADIGWTAPVNGTPPLSYSIYYS
                     TSNTAPTLTTAPTISGVTTNPKTITGLVASSTYYVWVRSICSATDSSSWTLVKQFATQ
                     CEAISIYPYTEGFNSVGALPACWTVYEGSVGATQHWTSVAADATHGSATAAEGAAFMS
                     MNYYNATTTYNPYYLQSIPFALGATAKQAKFALWMGATSGTDNLKFEASTDGGSTWTV
                     LATYTANSANSSSTSPWENATVDLSAYMNQTVIFRLNATSNWGSGFCNVGFDNFIIQN
                     TPSCNEPTAVTKANVTTSGADISWTAPVSGTPPLSYSIYYSTTNTTPALNAAPNVSGV
                     TGLMTTVSGLTPATTYYVWVRSICSATDSSNWSNVISLTTACTPATLPYTENFNSTSG
                     TSLPLCTSAENPGTGNTWVTTSSPGYGFSSRVLVYNYNGSNAANTWFYTKGLSLTAGT
                     TYDLTYKYGNNSTSYTEKMDVYYGNSAAFASMTELLADHTTINTATASTNTVSFTPTS
                     TGTYYIGFHAYSASNQYYLYVDDISVAEQVLPVTYTLFTGKKEGNTNILSWQTATETN
                     NIGFELQRSADGKNFSKLDFVNSKSVDGNSNATLNYSYNDIKPLGGNNYYRLKQLDKD
                     GKVNYSNTIVLNAGKVQQISIASVYPNPVRNTVNVQIVSPAIETVSLIVTDITGKVLI
                     QKNTALAAGDNTKQIDVSALSQGTYLIKAICANGCETTVFKFAKY"
     gene            11066..12769
                     /locus_tag="PLU36_03300"
     CDS             11066..12769
                     /locus_tag="PLU36_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ45807.1"
                     /translation="MQTITPLLQINNLSINFLTANNTLVNVVKRISATIQKGEVVAIV
                     GESGSGKSVTSLAILQLLPQKTVRYTGGEILWNNNGKTINLLKQDKQALQQIRGKKIA
                     MIFQEPMTSLNPVLTCGYQVKEAIMLHENIALNEARKKVIDLFRKVKLDNPQDVFNKY
                     PHQLSGGQKQRVMIAMAICCNPDLLICDEPTTALDVTVQQSILHLIKELQLSNNMSVI
                     FITHDLGVVAEIADKIIVLYKGEIVESNTTENVLKYPTNAYTKALLACRPALYNKREK
                     LPVVSDFLNELNSQEVLKNIEQINTINKQEPLVEVKNLHVVYVTNKNLLGKATAKYTA
                     VKEVNFTIYKGETLGLVGESGCGKTTLGRTILNLIKPTSGEIIYHNLSNYKSGKERKV
                     AFRRKMQLIFQDPYSSLNPAIAIGDAIAEPIIIHKLMPNAKKAKEQAIMLLQKVGLKE
                     MDYNKYPHEFSGGQRQRIVIARALAMQPEFIVCDESVSALDVSVQAQVLNLLNELKVE
                     FNFTVLFISHDLAVVKYISDRIMVMKSGEIVEMNDAEMIFNQPQSSYTKQLIEAIPTI
                     Q"
BASE COUNT         4354 a         1897 c         2215 g         4335 t
ORIGIN      
        1 ttgcttttga actgtattac attgctactt tctttttatg tagccaaaca aaaggttgtt
       61 aaaaataata tagaatctat acacacacaa gatttaatta accattatta tttatttaat
      121 tataatcctt atcaagcctc attagttgca caaaaaattg ctaaagcaac aaaaacaaat
      181 ttattttttc aggaaggatt tggagatagc ggtataaagt attatttaaa agaatctaat
      241 ataaattata gtgaattgca agaaagtact cttttaatag aaaataaatt tacattgctt
      301 accaataaca agaaatggat tgaagagcta ttggttaagc accattggaa tcttcaaaaa
      361 aaattaagca accttaacta ttatactgtt tatttatgca gtaaaaatta agttgggtaa
      421 ttatttttta ggtgtttggc tgtcttcgtt ttgtggttta tattcaaaaa caattttagc
      481 attcttctgc aagtcggagg ttaagaaaac ttgatagctt ttatttcctg ctttaatttt
      541 taataaagat gtattaggtg gaatttctcc taaactttca gcaactaaaa taaattcttg
      601 aacaggttca gaagcagtaa ttttaatatt tagtttaatt ggaatagtgc ttaacctttc
      661 attttgtata accggcacac cattgtggta aatagataca atatcattat ctatttgtcc
      721 attatcgtat aattctaaac ttatttcacc ttcgccaaca taaaaggttt ttactaaatt
      781 attttttctt tctgttaaaa ccttaggaag tggggttata ggtttttcta ttttttcaat
      841 tttctcaagt ggggcagtag gttgattgct ttctttcttt tcaattttat caaccagttt
      901 tggggttgct tcttttttta ttattggttt attgtttgta actaatgcat cttcggcacc
      961 tggcttatat tttaattttg ctgaatttat ttttttatta ggcgaagaat tttttgagga
     1021 tttgcttaga gatgagttag gtttattcac taaaaaatct tcctttccaa aatcactatc
     1081 tggtactttt tctaaatata caataccttc gccgcaatca ctttttgaag aatttattgt
     1141 gcttgaataa tctcctttta atatttcttt atctccaatt ttagaataag tcaaataaca
     1201 agtcattaag catggctctg aattgccagt catttttagc tctaacattt tactttcttt
     1261 gattagaaca ttgttagatt taacagtaaa aattccttgt aatgctgttt taccataaaa
     1321 tcttttgtct aaataagagt aagtaacacc ttgtagtttg ctggtttgag tattgtttat
     1381 ttgaacttcg tatttataac gttcttcatg aaattttttg gtaagtaaat caaattctct
     1441 ttgaataaag tacccacgcc aaacacctgt aatattttta gtatgtgagt tgtttttatt
     1501 tttattgttt gttgtagaat attgagcaaa agatatatta ctaaaagagt ttagtgaaaa
     1561 aaagaaaatt aagttgaaga ttattgtaaa attcaatctc atggtgcaaa tctaaattta
     1621 tatgcaatac tttacactca aagaatcgtt aaaggttatt tatttaaaag ttatttaatc
     1681 tttgtagttt ttatttttat ttattggcat ctaagtaata ttttcacggc tttataaatt
     1741 ttgattgaaa ttgaatgagc aataaggtgg caattattgg tgcaggtcca gcaggtttat
     1801 cagctgctta tttattaggc aaggcaggaa ttagtacaac tatttttgaa caggataagc
     1861 aatatgtggg tggtatttca agaactgaaa actataaagg atattatttt gatattggtg
     1921 gacataggtt ttttagcaag agcaaagagg tagaagattt ttggacagaa attttacagg
     1981 atgatttact ggaaaggcca agaagtagta gaatttatta caaccataca ttctttgcgt
     2041 atccattagt tgctctagaa gcactcaaaa aattagggct ttggcaatct tttctttgtg
     2101 ttgcaagtta tttaaaagca aaactttttc ctgtaaaaaa ccctcaaaat gtagaagact
     2161 gggtttctaa tcagtttggt aaaaaattat attctatttt ttttaaaaca tataccgaaa
     2221 aagtttgggg tataagctgt aaagatattt cggcagattg ggcagctcaa agaattaaag
     2281 gtttatcatt atcctctgca ataaaaaatg cattaataaa acctggttac gctagtaaag
     2341 aaaaggtaat taaaacatta atagattctt ttagatatcc taaaaaaggc ccaggaatga
     2401 tgtgggaaac ctgtagagat aaatgtttgc aattaggtgt taaagtaaaa atgaatgcag
     2461 aagttatagg tcttaatttt aatgataacc tttggcaagt agtcatgaaa aatgaacaat
     2521 ttgacgaaaa gtttacctac gtcctttcct cagcatctat aaaaaatata gtgccaaata
     2581 ttctaccttc agtaaacaaa gtagcaaaag aggctgcgtt acagttaaag tatagagatt
     2641 ttttaacagt agtattaatt tgtaaagata aagacagctt taccgataat tggatttata
     2701 ttcacgaccc atcagttaaa gttggaagaa ttcagaactt taaaagttgg agcccatata
     2761 tggtaccaga tgaaagtatg gcatgttatg gtttagagta tttttgtttt gaaggagatg
     2821 gcatgtggca aaaaaacaat gatgatttaa ttgatttagc aaccaatgaa atgcaccaaa
     2881 taggattatt aaacaaagaa aatgtagtag aggggtatgt agttaggcag cctaaagctt
     2941 atcctgtgta cgataaaaat tataaagaaa aagtgcagtt agttagagat ggattaaaac
     3001 cttataaagg tctttactta attggcagaa atggtatgca taaatataat aaccaagatc
     3061 atagtatgat gactgctatg tttgcagcta aaaatattat tgcaggaaaa gaattatatg
     3121 atgtttggaa agtaaaccaa gatgcagaat accaggaaga aggcgaaaga ggtgtaaatg
     3181 aaggaggacg gctaatacca attgtacaaa actaactttg attaaaaata ttttttaact
     3241 tataaaaaat tatgcaaaca acactttgct tcgattttgg gaatacaaga ttaaaatgtg
     3301 ctgtttttaa aggggcagag ctgctgcaag tagaggtatt agaaaatgat aaggtggcaa
     3361 acgttgaagt actattaaaa aaatatcaac cacaaaaatc aattttttcc tcagtaataa
     3421 atcataataa agcaatagaa gagttgttac aacaaaaaac tacttttcac caattaaatg
     3481 caaacagcaa attaccattt accacaccag ttggcaagcc agaaactatt ggtgccgata
     3541 gattggcaat ttgtgcagga gctatacatt tgtaccctaa taaccacaat ttggcaatag
     3601 gcttaggtac ttgtattact tttaatttca tcaataaatt tcatgagttt ttaggtggta
     3661 gcatttcacc aggcatgaac atgcgtttta aagcaatgca ccaacaaaca gctttattgc
     3721 ctttggtaga agctactcat aattttcctt taattggcta cgatactaaa actaatttat
     3781 taagtggtgt tttattaggt atggctaaag aaatagatgg tattatagaa gcttattcta
     3841 aaaaatataa caaatttaac gtgctaataa caggtggcga tatggctttt tttgtctctc
     3901 atcttaaaaa caagatattt gccgaccctt atttaatatt taaaggactt tatgcaatta
     3961 gcgaaaacaa taattaatag atttacagct actgtaattt ttacagtttg tttcatcttc
     4021 attacagcag cacaagaaaa ttcaccattt tccagatacg gattgggtga tttaacgaat
     4081 acttttcaaa ccttaaataa tggtatgggt ggtgttgctg tagcttataa tgatcttcaa
     4141 tctatcaact tcaataatcc tgcatctttt gccagtacca gaattgttac ttatgattta
     4201 ggtgttacaa tagattccag aatactaaga agtaaagctc cagtaaataa atacaattca
     4261 gccaattttt cacctgcata tattgcagtt gcaaccccaa ttagttctaa acactctttt
     4321 ggtttagctt ttggttttaa acctgtaact cgtataagct ataatattat tagaaatgaa
     4381 agattagcag gaattgatag tgttcaatct ttatttgaag gtacaggtgg catgaatgaa
     4441 gtatttgttg gggttggaaa aaaatggaaa aacttagcaa ttgggttatc tactggttat
     4501 cattttggta aaaagcaaaa caataccaaa cgtggtttta ttaacgatac ggtagtatat
     4561 tataaagcca attacaatac atctacaaca tttggtggag tctttttaat gggaggattt
     4621 caatacgata tacttttatc cgattctgct aagaatagaa aaataacaaa gcctattaaa
     4681 gatttttatt acttaaaatt aggtggtact ttttctctag gccatcaact aaatgcagag
     4741 caaacttcat ttaaagaaac atttgattat gactattctg gtggctctta cactattgat
     4801 agtttaaatg tgggtaatac tattaaaggt aaaattaaaa ttccagcaaa ttttggtttc
     4861 ggatttttat ttcaaaaaac ctctgtagat atttcaggcg tttatgatag atgggctatt
     4921 ggtgcagatt tacttttatc taattggaaa aattatcgtt tttacgataa aaaagatgct
     4981 actgtaaata gttggcaatt taaaatagga gggcaatgga caccagatgc taaaaatacc
     5041 aaaagctatt tcagtagagt agcttataga gcaggattga atgtaggtaa agattatatt
     5101 aatgctgacg gaaaagaaat gaaaactttt tctttttcta ttggactagg tttgcctgta
     5161 cgttctacaa ggtttagcta ccaatattcc acaattcata cagcattaga aataggtaaa
     5221 agaggtagtg cagcaaacaa cataaccgaa aatttcttta aactttctgt tggctttagc
     5281 ttaagtgata tttggtttat taaacgtaaa tatgattagc aattttaaac atattacttt
     5341 ccaaaaggca gtcttagtgg taggctgctt ttttgtgttt tcttgcgaaa ataaaataga
     5401 aactgtaaga gatttaggaa aagcaacttt aggagtagaa gaagggaaga atatagaaag
     5461 ctttattagc caatctggta aaatgagagc aaaactaaat gctccaatta tgttaaggta
     5521 tttattagat acccctaaaa tagaatttac taaaactttg tttgtaagtt tttatgatga
     5581 tacgcttggt attgaaagca atttgtttgc taaatatggc aggtatttag aaaatgataa
     5641 taaagtttat ttaaaagata gtgtagtggt ttacaatatt gctggcgata ctttatggac
     5701 ttctgagttg tactggaatc aggatacaga agagtttttc actaataaac cagtaagagt
     5761 gaaacgagag tttaataata agtatattca tagtataggt gtaagagcta accaaaattt
     5821 aaaagacatc acattttata aaattcagcc tgatagttat tttattgttt cagatagtat
     5881 ctattgagta atttcccatc tgttttcttt acatttttaa cgcttgtata aaattaatag
     5941 gaggttatcc tctttttatt ttttttttaa aaaaaaagtt atatcattga agccttaaaa
     6001 ttaacttatt atgagaaaaa tgttactctt gcttacttgt ttaattgtat tgtattcaac
     6061 aagtatagcc cagtattgca actcttcttt tagtaatgca tcttttgagc acattactaa
     6121 tgtaactttt ggtggggtca ataattcttc tactggtaat acaggaggcc ctgtaaatta
     6181 tttaaataag gttgcttatg tttattacaa caattctcat aatttgtcgg tttccatttt
     6241 agctgatgca agtgaatatg tgtatgcatt tatagattgg aaccaagatg gtgattttgc
     6301 agattcagat gaaacttata cagttgcttc caatgtttca actactggac cttttgttct
     6361 aagtatagct actcctttaa ctgcacttgc aggaaataca agaatgagag tgatggttga
     6421 ctataacgga tccactccag acccatgtaa aaattctact tatggcgaag cagaagatta
     6481 tacagtaaat gtaggcaaca tgcctgcttg tatgccccct acaaatttag ctgcaaatcc
     6541 agttggcata agtacagcat cggtttcttg ggatgcttct gtatcaaacc ctactggtgg
     6601 ctatcaattt gagttaagaa caagtggaac accaggtagt ggtattgctg gattagatac
     6661 aacagctttc acaacagcat taacagcaga ttttactgaa ttaaccattt ctaccactta
     6721 taacttatat gttagagcta tttgtggtgc aggcgattca agtacctggg caccttatac
     6781 tttttctaca gtatatgctc aagcaaggcc atggatagaa ccttttgatg tgctaactgt
     6841 tccaacaggt tggtcaacta ctgggtttac aattggcact tcctcttcta ttccagatga
     6901 gcaaacaaat attttataca gaaacttatg gagtggagca ccaaatggta ctttttcatc
     6961 agtaagtgtt ggtgcaattt tagctggcga tgcattaacg tttaattatc aacttagtga
     7021 ctattcttct ccttatggtc ctcctgcagc aaactcttgt agttttgttg tttctatttc
     7081 ttcggatttt ggagttactt ataccgatgt tgctactgta attaatgatg gcacagctgg
     7141 ttggaagaca tttacacaag atttagcagc ttatattggc cagtatataa gtgtaaaagt
     7201 tactgccact tggctaacag gcgattatta tttatcgttc gataattttg ctgtacaaag
     7261 ctgtatgcct ccaacaaatt tagaaattga tccgcttaca tttactgact caacagcaac
     7321 aataacttgg gatgcctcaa tatctacacc agctttgggt tataaatggg agttaagaac
     7381 tagtggtgca gctggtagtg gccctgttgg tttagtagcc agtgataata ctaatttact
     7441 aacaaaagca cttactggtt taacaagcaa tacaaactat acttttagta ttttagcaaa
     7501 atgtagtgtt accgattcta gtacttgggc tacaaaaatg tttacaaccc catgttcatt
     7561 agtatcttat tttaatgaat cttttgatgc agttactgta ccagcattgc cagattgttg
     7621 gttaaaaatg ggtgctggct cagcttatac tcaagttggt acttcttttt ctgctcctaa
     7681 tagcatttat atgtactcta gttcaggaaa cattgcatat ttaagaatgc ctcctgttag
     7741 tggctctgat ttaagtacac atagattaag gtttaatgct caagcaaatg gtacactggg
     7801 tggaacaatt caagttggtt atttaactga tccaaatgat gtttctactt ttgtgcaaat
     7861 aggaacagat tttacaccta ccagtactac atcttttgat aattttgtac ttagcccagt
     7921 tacaactata acaggtgtaa atacacttgt ttttaaacat acaggtactc ctagctatgc
     7981 agtatttatt gataatgtaa tttatgagcc aataccaaat tgcgaagaac caactaatgt
     8041 atctacttct aatattacac ctacaactgt agatttaagt tgggatgctc ctacctcagg
     8101 aactccacct ttatcttaca gtatttatta tagtacagta gatgcaacac cttcattaac
     8161 tgctacgcca aacgtttcag gtgttttggg tacaactgct accttaacaa acttaacagc
     8221 tgcaacaacc tattatgtat ttgtacgtag tatttgtagt gcaactgatt caagtacttg
     8281 gagcttcaaa aaatcattta cctctgcttg tgtagcaatt acttctttgc catggacaga
     8341 aggttttgaa ggtctttcaa cttttggtgc aggtgtagtt ccagcttgct ggaatatcga
     8401 aaatatttca ggtactaaaa ttactagtgc taatacgcct attatgaatg gtattggacc
     8461 tagaacaggt actaattatg tttggagcag atatggtact aataactgga tttttgcacc
     8521 tgctatacaa ttaactgctg ggcagtctta cgatttctcc ttcaattatg taaatgttga
     8581 tgcaacttca ggttttacag tggatgtggc ttggggtagc aatcctgcaa gtgtagatat
     8641 ggtaaaccct ttaggaacta ttagtaaccc tactaacaat acttatgctc aggcaaaata
     8701 ttcctttaca ccatctacta cggatgtata ttattttggt gtgaaagtga atagcacatt
     8761 tgctccttgg tatttatgtt tagatgattt taaagtggaa ctttcaccaa cttgtaacga
     8821 accaacagat gttacggtga gtaatattac aaccaatagt gcagatatag gatggacagc
     8881 cccagttaat ggtacaccac ctttatctta tagtatttat tatagtacat ctaatactgc
     8941 acctacttta actacagcac ctaccataag tggtgttaca actaatccta aaactataac
     9001 tggtttggtt gcatcttcaa cttattatgt ttgggttcgt agcatttgta gtgcaacaga
     9061 ttctagtagt tggactttag taaaacaatt tgctactcag tgcgaagcta tttctattta
     9121 tccttacacc gaaggcttta attcagttgg tgcattacca gcttgttgga cagtttatga
     9181 aggttcagtt ggtgctaccc aacattggac ttctgttgct gctgatgcta ctcatggatc
     9241 tgcaactgct gctgaaggtg ctgcattcat gagtatgaat tattacaatg caactactac
     9301 ttataaccct tattatttgc aatcaattcc atttgcattg ggtgctactg caaaacaagc
     9361 aaaatttgca ctttggatgg gagcaacaag tggaacagac aatttaaaat ttgaagcatc
     9421 aaccgatggt ggtagtacgt ggacagtttt agcaacatat actgcaaact ctgctaattc
     9481 atcaagtaca tctccttggg agaatgcaac tgttgattta tctgcttata tgaaccaaac
     9541 agttatattt agattaaatg caacatcaaa ttggggttca ggtttctgta atgttggttt
     9601 tgataatttt attatacaaa atactccatc ttgtaacgaa ccaactgctg ttacaaaagc
     9661 taatgttact acttctggtg cagacataag ctggacagct cctgtttctg gtacaccacc
     9721 tctttcttat agtatttatt acagcacaac aaatacaaca ccagctttaa atgctgctcc
     9781 aaatgtaagt ggcgttacag ggttaatgac tacagtttct ggtttaactc ctgctactac
     9841 atattatgtt tgggtacgta gtatttgtag tgcaacagat tctagtaatt ggagtaatgt
     9901 tatttcttta actactgctt gtactcctgc aacattgcct tatacagaaa actttaacag
     9961 tacatctggt acaagtttac ctttatgtac atctgcagaa aatcctggaa ctggtaatac
    10021 ttgggtaaca acaagtagcc caggttatgg ctttagttca cgtgtattag tatataatta
    10081 taatggatca aatgctgcca atacttggtt ttatactaaa gggttaagct taactgctgg
    10141 aacaacttat gacttaacct ataaatatgg aaacaacagc acatcttata cagaaaaaat
    10201 ggatgtttat tatggcaact ctgctgcttt tgcttcaatg acagagcttt tagcagacca
    10261 tacaaccatt aatactgcaa ctgctagtac aaatacagta tcctttacac caacaagtac
    10321 aggtacttat tatattggtt tccatgctta ttcagcaagt aatcaatatt atttatatgt
    10381 tgatgacatt tcggttgctg aacaagtatt gcctgtaact tacacattat ttactggtaa
    10441 aaaagaagga aatactaata tattaagttg gcaaactgca actgaaacga ataatattgg
    10501 ttttgagttg caacgcagtg ccgatggtaa aaacttcagc aaattagatt ttgtaaactc
    10561 aaaatctgta gatggtaata gcaatgcaac attaaattat agttataacg atattaaacc
    10621 attaggtggc aataattact atcgtttaaa acaattagat aaagatggta aagtaaacta
    10681 ttcaaatacc attgttctta atgcaggaaa agtgcaacaa attagtattg ctagcgtata
    10741 tcctaaccca gttagaaata cagtgaatgt acaaattgtt tcacctgcaa tagaaactgt
    10801 tagtttaata gttactgata ttacaggtaa agtattaatt caaaaaaata ctgcattagc
    10861 tgcaggagat aatactaaac aaattgatgt gagtgcttta tcacaaggta cttatttaat
    10921 aaaagcaatt tgtgcaaatg gttgcgaaac aactgttttc aaatttgcta agtattaatt
    10981 taatttcctt ttattattga tatacgagct acgcattgcg tagctcgtat tgtttaatac
    11041 taatgaatat tccttacatt gcatcatgca aaccattact cctttattac aaattaataa
    11101 tttatcaatt aattttttaa cggctaataa tacgttggta aatgttgtaa aaagaattag
    11161 tgcaaccata caaaaaggag aagtggtggc tattgtgggc gaaagtggct ctggcaaatc
    11221 cgttacttct ttagccatac tacaattatt accacaaaaa acagttcgtt atactggtgg
    11281 cgaaatttta tggaacaata atggcaaaac catcaacttg ttgaagcaag acaaacaggc
    11341 tttgcaacaa attagaggta aaaaaatagc catgattttt caagaaccaa tgacttcttt
    11401 aaaccctgtg ttaacctgtg gctatcaggt aaaagaagca attatgttgc atgaaaatat
    11461 tgcattgaat gaagcaagaa aaaaagtaat tgatttattt cgtaaagtaa aattagataa
    11521 tccccaagat gtatttaata aataccctca tcaattaagt ggtggacaaa aacaaagagt
    11581 gatgattgct atggcaattt gttgtaatcc tgatttgctt atttgtgatg aaccaactac
    11641 tgctttagat gtaacagttc aacaaagcat attacattta ataaaggaac ttcagctttc
    11701 taataacatg agtgtaattt ttataacaca cgacttagga gtagttgcag aaattgctga
    11761 taaaattata gtgctatata aaggagaaat tgtagaatca aatactaccg agaatgtttt
    11821 aaaataccct actaatgctt atacaaaagc cttacttgcc tgcaggcctg ctttatataa
    11881 taaaagggaa aaactaccag ttgtttccga ttttttaaac gaattaaact cacaggaagt
    11941 attaaaaaat attgaacaga taaatacaat aaacaaacaa gagcctttag tggaagtaaa
    12001 aaacttacat gtagtttatg taactaataa aaatttatta ggaaaagcaa ctgcaaaata
    12061 tacagcagtt aaggaggtga actttacaat ttataaagga gaaaccttgg ggctagtagg
    12121 agaaagtggt tgtggcaaaa caacattagg cagaacaatt ttaaatttaa ttaaaccaac
    12181 aagtggagaa attatttatc ataatttaag taattataaa tcgggcaaag aaagaaaagt
    12241 agcatttaga agaaaaatgc aattgatttt tcaagaccca tattcttcct taaatcctgc
    12301 aattgcaata ggagatgcca ttgcagagcc aatcataata cataaactta tgcctaatgc
    12361 aaaaaaggca aaagagcaag caattatgtt attacaaaaa gttggattaa aagagatgga
    12421 ttataataaa tacccacatg agtttagtgg agggcaaagg caaagaattg taattgcaag
    12481 ggctttagcc atgcagccag aatttattgt ttgcgatgaa agtgtaagtg ctttagatgt
    12541 tagtgttcag gctcaggttt taaatttact taacgagtta aaagttgaat ttaattttac
    12601 agttttattt atatcgcatg atttagcagt tgtaaaatat attagtgata gaataatggt
    12661 gatgaaaagt ggcgaaatag tagagatgaa tgatgctgag atgattttta atcaaccaca
    12721 aagcagttat actaaacaat taattgaggc aataccaacc attcaataaa atatgtttgg
    12781 tgaataatga atagaaagta c
//
LOCUS       DAOQPK010000040        12790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_2527_length_12790_cov_4.240361, whole genome shotgun sequence.
ACCESSION   DAOQPK010000040 DAOQPK010000000
VERSION     DAOQPK010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12790
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2527_length_12790_cov_4.240361"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..894)
                     /locus_tag="PLU36_03305"
     CDS             complement(163..894)
                     /locus_tag="PLU36_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45808.1"
                     /translation="MLSAAVIAFVGCQKTSTEKQEQGIVGTTEVSANSLGMSVNSSYG
                     FLVFTTTDDVDAYLDYLKANTHADVQENLSSIGFSNSLGATLYDEEYATELVTEEQAI
                     NYIVNTHRIFQVQDVIMKPIVAWTGCTTAKWKYLLTMIDSYLDNSSYENLIANYYDEN
                     TMNKFAAGQEQKTSIFEQIQSTPNGYEDEEPTECPTAEAGRPIIGWGNWKCGKCDATG
                     HKSCAKKHYFLFWVDDVKYEYIQCN"
     gene            complement(971..1825)
                     /locus_tag="PLU36_03310"
     CDS             complement(971..1825)
                     /locus_tag="PLU36_03310"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMZ45809.1"
                     /translation="MKIILILFMPLITFAQKKCSGIVIDRITGSRIPYAAIGLIKENK
                     GINANEDGSFSIVSSFPQTDSLRISSVGYKALLLPVSTCVNEGVVMLQQEIRELKEVI
                     VSNRSKQTFILNKFRKCSWSIYKIGLNTISQLAQRFNAPAQGMQLTALELCKDDSESK
                     FRIRIYDIDSTYKYPSNDLVDSVIEVTSKEGHVRINLEDYNIIIPGKSFFIAIEWLYI
                     PFNIQLKKEKINGRRIVKAWYKPQIKLIERDQNAINEDIWELSFMGRWANRWVSKGVN
                     FQITAELR"
     gene            complement(1897..2700)
                     /locus_tag="PLU36_03315"
     CDS             complement(1897..2700)
                     /locus_tag="PLU36_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45810.1"
                     /translation="MKKLIVIFTVVLSAAVIAFVGCQKTSTEKQEQGIVGTTEVSANS
                     LGMSVNSSYGFLVFTTTDDVDAYLDYLKANTHADVQENLSSIGFSNSLGATLYDEEYA
                     TELVTEEQAINYIVNTHRIFQVQDVIMKPINEVNEEVKWEFLLTMVSSNLNNSSYENL
                     IANYYDENTMNKFATNWQDSTFDLLEFIDATPNGYEETEPNSTEALIPLYGRKYEYET
                     SCSQPHYNVLGNCVRDCQTIRRRVTHIWFITIKGPKYVESSWSETSEGC"
     gene            2985..3338
                     /locus_tag="PLU36_03320"
     CDS             2985..3338
                     /locus_tag="PLU36_03320"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ45811.1"
                     /translation="MYQTGNKIKKLRELRNFTQGYMAEKLGISQSNYARLENDEVGIS
                     NERLKQIADVLETTKENILSFDEKYVFNVHNEQHSSGAIVHNYSKSPEIKQLYEDKIK
                     LLEEKVQMLEKQLSS"
     gene            3499..3972
                     /gene="rpsP"
                     /locus_tag="PLU36_03325"
     CDS             3499..3972
                     /gene="rpsP"
                     /locus_tag="PLU36_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HMZ45812.1"
                     /translation="MAVKMRLQRHGSKKRPFYFIVVADARAPRDGKFIQKIGTYNPLT
                     VPASIQLDRQKALDWLHKGAQPTDTVRRILSFKGVLYLKHLLRGVKLGLFDDATAMTK
                     FQAWHEEHEAHVKRRNDSHRNTQRQKKAYVPPVRKVEEVAQPANDAPAAEENTEA"
     gene            complement(4030..4752)
                     /locus_tag="PLU36_03330"
     CDS             complement(4030..4752)
                     /locus_tag="PLU36_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45813.1"
                     /translation="MKYFINKKLLTLTVAFFFFTLAAIFIQLFAFQPVAKYFSYKTSI
                     SYDATGLCKSHPKDSFIERLYEQYAYEIENNLEFSGKFQLQKYERQQLIELILIDGAA
                     PLIFIILGIVGFVYLIKYKKNINYNNLSLIQWIAVFLNLFWFRWLLLLVLYIVFVSLS
                     KKDNALLIINETNIAYALNISPLFILIPLGLFSTWICYITVFVFIPKVYRGKFLLTGV
                     IGCILALLFWFKLIGAMVLPYE"
     gene            complement(4762..5778)
                     /locus_tag="PLU36_03335"
     CDS             complement(4762..5778)
                     /locus_tag="PLU36_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45814.1"
                     /translation="MNNRSFSNEGIFFSDILMPSESFLEIFDFLKSNISGLDALSTSN
                     LFRINIFLSQGDSLSNLQISNIKGFSIFCKNENSFSHHLFRVDNGVVIQGEQEVLCGG
                     EFQECSISDLRGIGYYLNDEISPSAIINLMNDIDIEFETDITKQYLSKLLYKTQYITP
                     EIYVEGDDFGPNCSHCGERNRKVSCKWTGKERECPSCIKDILSKTNVDLELGYGELSS
                     EDAHSFRDNFMAESNVGRKYIIYYYVLSDFALAEMPLTINSISDYYEFAIDCYAIKNI
                     IENGSNNKVVVNSNFKEKALAKIEYFKGVTTNIKILTILDDIKLDIINWHGLSKGALL
                     EALN"
     gene            6273..7193
                     /locus_tag="PLU36_03340"
     CDS             6273..7193
                     /locus_tag="PLU36_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HMZ45815.1"
                     /translation="MKQVLEILKLGQKNFSIPGMDLLYEKEQNIPGSINYNISRYYAQ
                     DAWLAEDTGMMVYHFNENKPEENFLELRFCASGNKYCFDKSCSFCEKQPTDCNGQQTT
                     LDVFSFHFSSTFLYQFTQNVKVTNRKDEVLAFKHPNAFTKVFPVCPKKRNVLDSLLNH
                     SYTGALENIFINAKIHELLLYSLDCLVDEKEEGFNCKFLEDERSRLAIYDARDILLKH
                     IGDPLTIKELSRKVAINECYLKKGFKEIFGTTIFDFYQQQRMEHAKYLLYEKSLSVTD
                     ASALLGYSSISHFSAAFKKHTGLKPCELLK"
     gene            complement(7196..10435)
                     /locus_tag="PLU36_03345"
     CDS             complement(7196..10435)
                     /locus_tag="PLU36_03345"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HMZ45816.1"
                     /translation="MKKIISLIILISCFITKINAQDLETELNSNISMYKKYVSEKNYT
                     AAIPYIKKSALLVKQKYGEQNKDYLTLINSTGTLYFFISKFDTAVLYYDTTLTLYKKY
                     YSADKEGLLQTHLNLAMSYNKMEDYTNAKKWYEIVVASQKELYGTNSKNYRMSYGNLA
                     NVYKNLGLYEEAEKMMLENIDYYEKMDSKSEAYEIALQNLAALYQKWGLHYWAAMYYF
                     IASDVRLVIAGENDTYFKNYISNATFYFEKAAGECRKDGDFERAEKCYNYNTIIFKER
                     KNNKEYARFLNLRGLMELELKKYTNAEISFFTALQIFKKEFDTTHLSVGMVYNNIGML
                     YFNLNRYDFAEDYCYEGYKIRLKRLKPNDKDIAESCCNLGLIYTYTMQYEKAEEFNLK
                     AKQIYENGIGTNNSEYAIALNNLGMLYWKKKNYKDAEKYYLQSLQLREKLYGKEHTSY
                     ARILNNLGTIYLEWENPTKAIKYFEQAVAISEKKFGKNHIRNIPYLNNLALCYNDLGN
                     YNEAKKIIINTNQLNSKLIKDNFTILTEYEKELYADEILYNSKYANSILYNSRNYNSE
                     NIIDNLNQQLQLKGLVLNETKTFYNYVKNNSDTAFQKMFNEYSQLKTRIAREYGFAIE
                     NRSKDLSSWEGRVDVLEKDLLLKSKKFTEQQKGIEVNYNTLKNSLKEDEVLIEFAKFN
                     LYNKGETDSVLYVAYIVTKNGNAPVYVPLFEESELQKILTSAGKTNQALASNLYRSTI
                     TKNKSKANFGNELYNLVWQPLEPYLKNKKKIYFAPVNKLNTIAFNALAIDSNTYLIDK
                     YQLFQYTSTRMFVQDKKEVNKVANIALFGNADFSLTQSTIKTYQQNADSAIVKRGFNK
                     ENTTLSTWATLPGTAEEIKNIGTLFSKKSIPQYVYTNTNASEENLKKLNDQNISIIHL
                     ATHGFFLPKIKETQQSISNSTTTNYNTAYDPMLRSGIVLSGANYAWNGGFVPSNKEDG
                     IVTAYEISQLNLSNTNLVVLSACETALGDIMGNEGVYGLQRAFKLAGVKNIILSLWQV
                     PDKETAELMTIFYNNYLSGKSINESFNLAQQSLRKKYSPYYWAAFVLIK"
     gene            10507..12141
                     /locus_tag="PLU36_03350"
     CDS             10507..12141
                     /locus_tag="PLU36_03350"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HMZ45817.1"
                     /translation="MIKLIFFILALTFIGCHTKENADLIVLNAKIYTVDSAFSLAEAM
                     AIKDGKILALGKAESILDSYVAKEKFDANGKFIYPGFIDAHAHFVGYGKSLFEVQLYD
                     SKSWNEVIGRVTRFAKNNPNEKWIIGRGWDQNKFEGKQFPTNELLNQLFPDVPVMVTR
                     VDGHAAIANAKALALAEIKPNQQLTGGSIETKNGKLTGLLIDNAVDLVQSKIPSAALK
                     DYDKWLMAAQENCFEQGLTTITDCGLMYNDVEKIDSLQQQNKLLMKLYVMLSDDQRNY
                     EKYLSKPPYKTNKLFVKGIKVYSDGALGSRGACLLQHYSDKPNWFGFLLSNKAHFDSL
                     ASILVNTPYQMCTHAIGDSANRTILTIYNKYLKGKNDKRWRIEHAQVINENDFDLFGK
                     ASVIPSVQPTHATSDMYWAEERLGKKRLQNAYAYKQLLKQNGWLPLGTDFPVEDISPF
                     KTFLAAVIRKDAVGFPTNGFQKENALTREEALRGMTIWAAKANFLENETGSLVVGKKA
                     DFILLNNDLMQVPETEILNTKVIATWSDGKKVYNKK"
     gene            complement(12154..>12790)
                     /locus_tag="PLU36_03355"
     CDS             complement(12154..>12790)
                     /locus_tag="PLU36_03355"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ45818.1"
                     /translation="PAGIEQIIQLQLQVANITLPFSLFFHVLHTEGILINENKFSVEC
                     FTVQHSIECWGFIFREKKAPRKIDKEKAVQYQIPAAFYNQLKEGLDYVTKEGLIINNK
                     DVTTTNIPARSYAYCADTKYFEELIPKVKDVNVLYHETTYLKDFEEKAILRFHSTTHQ
                     AANIAKQCNAHKLLIGHFSSKYENLDQFLTEAKEVFKSTELAIEGVCYNIL"
BASE COUNT         4319 a         2016 c         1845 g         4610 t
ORIGIN      
        1 tgtgttttca tcaataacaa atccagaaat agttgtttga ctataaactt caaaataaaa
       61 aaagtataaa aaaataatgc tcagtatttt catcatacgt ttaatattta aacccctgac
      121 aagatgtgtg aacttatgct ttgtcagggg ttggtattaa aatcaattac attgtatata
      181 ctcgtattta acatcgtcaa cccaaaatag gaaataatgt tttttcgcac atgacttatg
      241 tcctgttgca tcacacttgc cacatttcca attcccccaa ccaataatag gtcttcctgc
      301 ttcagcagta gggcattctg ttggctcttc atcttcatag ccattaggtg tagattgtat
      361 ttgttcaaat atggatgttt tctgttcttg accagcagca aatttattca tggtattttc
      421 atcgtaataa tttgcaatta aattttcgta gctgctatta tccaagtaac tatctatcat
      481 agtaagcaaa tatttccatt ttgcagtagt acaacctgtc catgctacta ttggtttcat
      541 tatcacatcc tgtacttgaa atatcctgtg tgtatttaca atataattaa tagcttgctc
      601 ctccgttaca agttcagtgg catattcttc atcgtacaag gtagcaccca aactattaga
      661 aaatcctatt gagctaaggt tttcctgcac atcggcatgg gtgtttgctt ttaaataatc
      721 aaggtatgca tctacatcat ctgttgtagt aaataccaaa aaaccgtaac tactatttac
      781 actcataccc aatgagtttg cacttacttc tgttgtgcca actattccct gttcttgttt
      841 ttcagtactc gttttttggc atccaacaaa ggcaattaca gcagcactta gcacaactgt
      901 aaaaataaca attaattttt ttatggttta gtgttttgta ctcttgggta atggataatg
      961 aaaaaaatta ctacctcaac tccgctgtaa tttgaaaatt gactcctttt gatacccacc
     1021 tgttagccca cctacccata aaactaagct cccaaatatc ctcatttatc gcattttggt
     1081 cacgctcaat aagtttaatt tgtggtttat accacgcttt cactattctt ctcccgttta
     1141 tcttctcttt ttttaactgt atattaaaag ggatataaag ccattctata gctataaaaa
     1201 aacttttacc tggaataatg atattgtagt cttcaagatt gattcgtaca tgaccttctt
     1261 tagatgttac ctctataaca ctgtcaacca aatcgtttga agggtattta taagtagagt
     1321 ctatatcgta aatacgaata cgaaatttac tctccgaatc gtctttacat agctccaatg
     1381 ccgtaagttg cattccttgt gcgggtgcat tgaaacgctg tgccaactgt gatatagtat
     1441 tcagcccaat tttataaata ctccaactgc atttcctaaa cttgttgaga ataaaagtct
     1501 gtttagaacg gtttgaaaca attacttctt taagttcccg aatttcttgt tgaagcatga
     1561 caacaccctc gtttacacat gttgaaacag gtaataaaag tgctttatag cctactgatg
     1621 atattctaag actatcagtt tgaggaaaag aagacacgat gctaaaactg ccgtcctcgt
     1681 tggcattaat tcctttattt tctttaataa gcccaatagc agcataaggg attctacttc
     1741 cagttattct atctataaca ataccagaac attttttctg agcaaatgta atcagaggca
     1801 tgaacagaat taagataatt ttcatcgtta atatttaatg taaaaagcta ggagcaacag
     1861 caaataagtt gccacatcta ctcataaatt gaaaactcag cagccttcag aagtctctga
     1921 ccaagagctt tccacatatt ttgggccttt gattgttata aaccaaatat gggtaactct
     1981 tcgtctaatc gtttggcaat cacgtacaca atttccgagt acattataat gtggttgact
     2041 gcaacttgtc tcatattcat attttcgtcc atacaaaggt atcaatgctt cagtagaatt
     2101 gggttctgtt tcttcataac cattaggtgt tgcatctata aattctaata aatcaaatgt
     2161 gctatcctgc caatttgtgg caaacttatt catggtattt tcatcgtaat aatttgcaat
     2221 taaattttcg tagctactat tgtttaggtt agatgaaacc attgttaata aaaattccca
     2281 ttttacttct tcatttactt cattaatggg tttcattatc acatcctgta cttgaaatat
     2341 cctgtgtgta tttacaatat aattaatagc ttgctcctcc gttacaagtt cagtggcata
     2401 ttcttcatcg tacaaggtag cacccaaact attagaaaat cctattgagc taaggttttc
     2461 ctgcacatcg gcatgggtgt ttgcttttaa ataatcaagg tatgcatcta catcatctgt
     2521 tgtagtaaat accaaaaaac cgtaactact atttacactc atacccaatg agtttgcact
     2581 tacttctgtt gtgccaacta ttccctgttc ttgtttttca gtactcgttt tttggcatcc
     2641 aacaaaggca attacagcag cacttagcac aactgtaaaa ataacaatta attttttcat
     2701 ggtttataat tttttaaaat tttaagtaat attttttgaa aatcagagct attgtttgta
     2761 gtctttggag gagatttatt gggttgtaac ataaaaatat agtttagggt ttttaaaaaa
     2821 ctgaacaaca aaacaggtat tccgctaaaa aaatttaatt aataactaaa tgttgttgtt
     2881 ggctttatct gttttgccaa tgctatattg cttatttttc tttacatttt taattgcata
     2941 aatgggtagt ttatgtattt tcgtacataa attatccaaa caatatgtac caaacaggca
     3001 ataaaataaa aaagctgaga gagctgcgaa actttacaca aggctatatg gcagagaaat
     3061 taggtatcag tcaaagtaat tatgcccgtt tagaaaatga tgaagtaggc ataagcaacg
     3121 aacgtcttaa acaaattgct gacgttttag aaactactaa agagaatata ttgagttttg
     3181 atgagaaata tgtgttcaac gttcataatg aacaacactc aagtggagct attgttcata
     3241 actattctaa aagtcctgaa ataaaacaac tatacgagga caaaataaag cttttggaag
     3301 aaaaagtaca aatgttagaa aaacaattaa gtagctaatt gtttaataat aacttaccga
     3361 acaaaggttt tacattaaac tttgttaaga gctaaaaaaa tctttggcag ccccaaaaaa
     3421 tacttataca ttcgctgccc aatcattcat gattgtatta ttaaccaaat tgttaaagcc
     3481 tttaaatttc tatttattat ggcagtaaaa atgagattac aaagacacgg cagtaaaaaa
     3541 cgccctttct attttatagt agtggcagat gcacgtgcac ccagagatgg taagtttatt
     3601 caaaaaattg gaacttacaa tccattaact gttcctgcaa gcattcagtt agatcgtcaa
     3661 aaagcattgg attggctaca caaaggagca caaccaaccg atacggttcg caggattctc
     3721 tctttcaaag gagtgttgta tttaaagcat ttattgcgtg gtgtaaaact gggcttgttc
     3781 gatgatgcta cagctatgac taagtttcag gcatggcatg aagaacatga agctcatgta
     3841 aaacgtagaa acgattctca tcgtaataca caacgtcaaa agaaagctta tgtacctcct
     3901 gttcgtaaag tagaagaagt agcacagcct gcaaatgatg cacctgctgc tgaagaaaat
     3961 acagaagcgt agcaatagca acgtaatttt cccaaaaatc cctgcttaaa aatttaagca
     4021 gggatttttc tattcataag gtaaaaccat tgctcctata agtttaaacc agaatagcaa
     4081 cgctaaaata cagcctatta ctcctgttaa taaaaatttg cctcgatata ctttgggaat
     4141 aaaaacaaaa acagtaatat aacatatcca agtagaaaac aaacccaatg gtatgagaat
     4201 gaacaatgga gatatattta atgcgtaagc tatattcgtt tcatttatta ttaaaagagc
     4261 attgtctttt ttacttaaac tcacaaaaac tatgtatagt acgagaagta ataaccatct
     4321 gaaccaaaat aaattaagaa aaacagctat ccattgtatt aaggacaagt tgttatagtt
     4381 aatatttttc ttatacttta tcagataaac aaaacccact atacccaaga ttataaatat
     4441 tagtggtgct gctccatcta tcaatattag ttcaataagt tgctgccttt cgtatttttg
     4501 taattgaaac tttcctgaaa attcaagatt attttctatt tcataagcat actgttcgta
     4561 tagtctttca ataaaactat cttttggatg gcttttacat aaacctgttg catcataact
     4621 aatacttgtt ttgtatgaaa aatattttgc aacaggttga aaggcaaaaa gctgaatgaa
     4681 aatagcagct aatgtgaaaa agaaaaatgc aacagttaag gtaagaagct ttttatttat
     4741 aaaatatttc attcaatatt tttaatttaa tgcttctaat aatgctcctt tagatagtcc
     4801 atgccagtta ataatatcca gcttaatatc atctaaaata gtcaaaattt taatattcgt
     4861 tgtaaccccc ttaaaatact caattttagc taaagctttt tctttgaaat ttgaattaac
     4921 tacaacttta ttattacttc cattttcaat aatatttttg attgcgtagc aatcaatagc
     4981 aaactcgtaa taatctgaga tgctattaat ggttaatggc atttcagcta aagcaaaatc
     5041 tgataacaca taataatata ttatatactt tctgcccaca ttagattctg ccataaaatt
     5101 atctctaaaa gaatgagcat cttctgacga taattctcca taaccaagtt ctaaatctac
     5161 attcgtttta gataatatat cttttataca actagggcat tccctttcct tccctgtcca
     5221 cttacaacta actttacgat tcctctcacc acagtggcta caattaggac cgaaatcatc
     5281 accttctaca tatatttcag gtgtaatata ttgggttttg taaagtaact tacttaaata
     5341 ttgcttagtt atgtcagttt caaattcaat atcaatatca ttcataagat ttattattgc
     5401 acttggagat atttcgtcat ttaagtaata cccaattcct cttaaatcag aaatactaca
     5461 ttcttgaaat tcacctccac ataaaacttc ttgttcgcct tgtattacaa ctccattgtc
     5521 aactctgaat aagtgatggg aaaaggagtt ttcgttttta cagaaaatac taaacccttt
     5581 aatattagaa atttgtagat tacttaagct atcgccttgt gataaaaaaa tgtttattct
     5641 gaaaagattt gaagtagaaa gagcatctaa accagaaata ttggacttta aaaaatcaaa
     5701 tatttctaag aaagattcag aaggcattaa aatatcacta aaaaaaatac cctcatttga
     5761 aaaactccga ttattcatat tatttgtaaa aaatcagata cttttctgct cataaaattt
     5821 tgaagaattt actactatat attctcttcc aaaaagtttt tctttgaata tttgcttttc
     5881 agtagtatta tatatgtttt tctccttaaa acaagaaaca aatataatag tacaaagtac
     5941 tgtaagaata gcaatagcag tcagcgactt tcctaaaatg ggtttctttt tcatatttta
     6001 tagtttagtt atccaaatgt aagcaatcta attaactaga ttaactatct ttaacagttt
     6061 tagagccacc tgtttaaaat ttaatctata aatcctgtat tgttcaaaaa caaccgattt
     6121 taagtaccct tttgtatcta ttttagtttt tttaaagcca taaaaagcat taaaatgaac
     6181 caatagtact tgaaaattaa aattttaaac aggctctaaa aataaaaaaa cttggaattt
     6241 acatgtatat acattaattt tgtactcttg aaatgaagca agtattagaa atattaaaat
     6301 taggtcaaaa aaattttagt atcccaggta tggatttgtt gtatgaaaaa gaacaaaaca
     6361 ttccgggttc tattaattat aatattagta gatactacgc tcaagatgca tggcttgcag
     6421 aagatacagg tatgatggtt taccacttta atgaaaacaa acctgaagaa aattttttag
     6481 aattacgttt ttgtgcttcc ggaaataaat attgttttga taaaagttgt agcttttgcg
     6541 aaaaacaacc aaccgattgt aatggacaac aaacaacctt agatgttttt tcttttcact
     6601 tctcttctac attcttgtat cagtttacac aaaatgtaaa agtaaccaac agaaaagatg
     6661 aggttttggc atttaagcac cctaatgctt ttacaaaagt ttttcctgtg tgtcctaaaa
     6721 agagaaatgt gttagattct ctattaaatc atagttatac cggtgcttta gaaaacatat
     6781 ttatcaatgc caaaattcat gaacttttac tttatagttt agattgcctg gtagatgaaa
     6841 aagaagaagg atttaattgt aagtttttag aagatgaacg tagccgattg gcaatttacg
     6901 atgcaaggga tatattatta aaacatattg gagacccttt aacaataaaa gaattaagcc
     6961 gaaaagttgc tataaacgaa tgttatttaa agaaagggtt taaagaaatt tttggtacta
     7021 ccattttcga tttttatcaa caacaaagaa tggaacatgc taaatatttg ttgtacgaaa
     7081 aaagtttaag tgtaacagat gcatctgcat tattaggcta ttcatctata tctcattttt
     7141 ctgcagcatt taaaaaacat actggtttaa aaccttgtga actgctaaaa taattttatt
     7201 ttatcaatac aaatgctgcc caataatatg ggctgtattt ttttctcaaa ctttgttggg
     7261 ctaaattaaa agattcattg attgattttc cagataaata attgttgtaa aatattgtca
     7321 ttaattcagc agtttcttta tctggtactt gccataaact taaaataata ttttttacac
     7381 cagccaattt aaaagctctt tgtaaaccat aaacaccttc attgcccata atatcgccta
     7441 aagctgtttc gcatgcactt agtacaacca ggttagtatt gcttaaattt aattgagata
     7501 tttcataagc tgtaacaata ccatcttctt tattacttgg tacaaaacca ccattccagg
     7561 catagtttgc cccacttaat acaataccac ttcttaacat tgggtcgtaa gcagtattgt
     7621 aatttgtagt tgtactgtta gagattgatt gttgcgtttc tttaatttta ggaagaaaaa
     7681 aaccatgtgt tgcaagatga ataattgaaa tattttggtc gttcaatttt tttaaatttt
     7741 cttccgatgc gttggtattt gtataaacat attgtggtat agattttttg ctgaacaaag
     7801 tgccaatatt tttaatttct tctgcagtgc caggtagggt agcccatgtt gataaagttg
     7861 tattttcttt attaaaacct cttttaacaa ttgctgaatc ggcattttgt tgataagttt
     7921 taatagtact ttgcgtaagg ctgaaatctg catttccaaa aagtgcaata ttggctactt
     7981 tatttacttc ttttttatct tgcacaaaca ttctggtaga tgtgtactga aacaactgat
     8041 atttatcaat taaataagta ttgctatcta tagctaaagc attaaatgca atagtgttta
     8101 atttatttac tggtgcaaaa tatattttct ttttattttt taaatatggc tctaatggtt
     8161 gccaaaccaa attatataat tcatttccaa aatttgcttt agatttattt tttgtgatag
     8221 tacttctata aagattactt gccaaagcct gattggtttt gcctgcactt gttagtattt
     8281 tttgtaattc tgattcttca aataaaggaa catatacagg agcattacca ttttttgtta
     8341 ctatatatgc aacatataaa acagaatctg tttcgccttt attatataaa ttaaatttcg
     8401 caaattcaat cagcacttcg tcctctttca aactgttttt tagtgtatta taattaacct
     8461 caataccttt ttgttgttcc gtaaattttt tactttttaa taataaatct ttttctaaaa
     8521 catctactct tccttcccat gaacttaaat ctttcgacct gttttcaata gcaaatccat
     8581 attctctggc tattcttgtt tttaactggc tatattcatt aaacattttt tgaaaagctg
     8641 tatccgaatt attttttaca taattataaa atgtttttgt ttcgtttaat accaaacctt
     8701 ttaattgtaa ttgttgattt aagttgtcta ttatgttttc gctattataa tttctactat
     8761 tgtataaaat actgtttgca tattttgaat tatataaaat ttcatctgca tacagttctt
     8821 tttcgtattc agtaagtata gtaaaattat cttttattag tttactgtta agttggttag
     8881 tattaattat tatttttttt gcttcattgt aattacctaa gtcgttatag cataaagcaa
     8941 gattatttaa gtaaggaata ttacgaatat ggtttttacc aaattttttt tcagaaatag
     9001 ctacagcttg ctcaaaatat ttaatagctt ttgtggggtt ttcccattca aggtatatag
     9061 tgcctaaatt atttaatatt ctggcgtaag aagtgtgttc tttgccatat aatttttctc
     9121 ttaattgtaa gctttgtaaa tagtattttt ctgcatcttt ataatttttt ttcttccagt
     9181 ataacatgcc caaattgttt aatgcaatgg catattcact attgtttgtg ccaattccat
     9241 tttcataaat ttgcttggct tttaaattaa attcttccgc cttttcatat tgcattgtgt
     9301 aggtatatat taaacctaaa ttacaacaac tttcggcaat atctttatcg tttggcttaa
     9361 gccttttcaa tcttattttg tagccttcat aacaataatc ttctgcaaaa tcgtatctgt
     9421 taaggttaaa atataacata ccaatgttat tatataccat gccaacagat aaatgtgttg
     9481 tatcaaactc ttttttaaaa atttgaaggg ctgtaaaaaa actaatttcg gcattggtat
     9541 atttttttag ttctagttcc attaatcctc ttaggttcaa aaatcttgca tactctttat
     9601 tattttttct ttctttaaaa atgatagtat tgtaattata gcatttttca gccctttcaa
     9661 aatcaccatc ttttctacat tctcctgctg ccttttcaaa ataaaaagtt gcattagata
     9721 tataattttt aaagtaggta tcattttcac cagctataac aagcctaaca tcagaagcaa
     9781 taaaataata cattgctgcc caataatgca agccccattt ttgataaagt gctgctaagt
     9841 tttgtaatgc aatttcatag gcttcgcttt tagaatccat tttttcataa tagtcaatat
     9901 tttctaacat cattttttct gcttcttcat acagccctaa attcttatac acattggcta
     9961 agtttccata actcattcta taatttttac tatttgttcc atacagttct ttttgagatg
    10021 ccaccacaat ttcataccat tttttagcat tggtataatc ttccatttta ttataactca
    10081 tggctaagtt gaggtgcgtt tgtaaaagcc cttctttatc tgctgaataa tattttttat
    10141 ataaggttaa tgtagtatcg taatacaaaa ctgctgtatc aaactttgat ataaaaaaat
    10201 acaaagtgcc tgttgaattt attaaagtca aataatcttt attttgttct ccatactttt
    10261 gttttacaag taatgcagat ttttttatgt aaggaatagc agcagtataa tttttttcag
    10321 aaacatattt tttatacata ctgatattgc tatttaattc agtttctaaa tcttgtgcat
    10381 ttatttttgt aataaagcaa cttattaaga ttattaaaga gataattttt ttcattgata
    10441 ataaatatta ccgaagttat aaatttgaat taataatttc aatacccact aaacagtata
    10501 ttttttatga ttaagttaat ttttttcatt cttgcattaa cctttatagg ttgccataca
    10561 aaagaaaatg cagatttaat tgtattgaat gctaaaattt atacagtgga tagtgctttt
    10621 tctttagctg aggcaatggc tataaaagat ggtaaaatac ttgcattggg taaagcagaa
    10681 agtattttag atagttatgt tgctaaagaa aagtttgatg caaatggaaa atttatttac
    10741 cctggtttta ttgatgcaca tgcacatttt gtaggttatg gtaaaagctt gtttgaagta
    10801 caattatacg atagcaagtc ctggaatgaa gtgataggaa gggttacaag gtttgcaaaa
    10861 aataatccta atgaaaaatg gataataggc agaggttggg accaaaataa gtttgagggt
    10921 aaacaatttc caaccaatga attattaaat caattatttc ctgatgttcc tgttatggta
    10981 acaagagtag atggccatgc agccattgca aatgcaaaag ccttagcact tgcagaaatt
    11041 aagccaaatc aacaattaac aggaggaagt attgaaacta aaaatggaaa acttacagga
    11101 ctattaattg acaatgcagt agatttagta caatctaaaa taccaagtgc agctttaaaa
    11161 gattatgata aatggttaat ggctgcacaa gaaaattgtt ttgaacaagg cttaactacc
    11221 attacagatt gtggcttaat gtataacgat gtggaaaaaa tagattcttt acagcaacaa
    11281 aataaattgt tgatgaagct atatgtaatg ttaagtgatg atcaaagaaa ctatgaaaag
    11341 tatttgagca aaccacctta taaaacaaat aagctatttg tgaaaggaat taaagtgtat
    11401 agtgatggtg ctttaggtag ccgtggtgct tgtctgctac agcattattc agataaacca
    11461 aactggtttg gatttttatt aagcaacaaa gcacattttg attctttggc ttctatttta
    11521 gtgaatacac cctatcaaat gtgtacacat gccattggtg atagtgctaa ccgtactatt
    11581 ttaactattt acaataaata tttaaaaggc aaaaatgata aacgttggcg tatagagcat
    11641 gcccaagtta ttaatgaaaa tgattttgat ttatttggca aagcttctgt tattccttct
    11701 gtacagccta cacatgccac aagcgatatg tattgggcag aagaaaggtt agggaaaaaa
    11761 agactacaaa atgcttatgc ttataaacaa ttattaaagc aaaatggttg gctgccttta
    11821 ggtactgatt ttcctgtgga agatatttct ccttttaaaa cttttttagc agcagttata
    11881 agaaaagatg cagtaggctt tccgactaat ggttttcaaa aagaaaatgc tttaaccaga
    11941 gaagaagcac ttaggggaat gacaatttgg gcagctaaag caaattttct agaaaatgaa
    12001 actggaagtt tagtggtagg gaagaaagcc gattttattt tgctaaacaa cgatttgatg
    12061 caagttcctg aaactgaaat tttaaataca aaagttattg caacctggag tgatggcaaa
    12121 aaagtttata ataaaaaata actttaacta agtctataaa atattataac aaacaccttc
    12181 aatagctaat tcagtagatt tgaaaacttc ttttgcttct gttaaaaatt ggtctaaatt
    12241 ctcatattta ctactaaaat gcccaattaa taatttatgt gcattgcatt gtttggcaat
    12301 gttagctgcc tggtgtgttg tagaatggaa acgtaaaatt gccttttctt caaaatcttt
    12361 taaataagtt gtttcgtggt ataatacatt tacatctttc actttaggta tcaactcttc
    12421 aaaatatttt gtatcagcac aataggcata agacctggca ggtatattag ttgtagttac
    12481 atctttatta ttaataatta aaccttcttt agttacataa tctaaccctt cttttaattg
    12541 gttgtaaaag gctgcaggaa tttgatattg aactgctttt tctttatcaa tttttcttgg
    12601 tgctttcttc tctctaaaaa taaagcccca acattcaata ctgtgttgta ctgtaaaaca
    12661 ttcaacagaa aatttatttt cattaattaa aattccttct gtatgcaata catgaaaaaa
    12721 taaagaaaat ggtaaggtga tattggcaac ttgtaattgt agttgtataa tttgttctat
    12781 tccagcaggt
//
LOCUS       DAOQPK010000041        12721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_2540_length_12721_cov_4.720512, whole genome shotgun sequence.
ACCESSION   DAOQPK010000041 DAOQPK010000000
VERSION     DAOQPK010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12721
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2540_length_12721_cov_4.720512"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..683)
                     /locus_tag="PLU36_03360"
     CDS             complement(36..683)
                     /locus_tag="PLU36_03360"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HMZ45819.1"
                     /translation="MQFSHLHTHTQYSLLDGAASIESLYKKALRDNMPAVAITDHGNM
                     FGAFSFVAEAWKKTKVVGKTEDGKEKLEPIVKPIVGCEFYVVENRHIRSFTKGAKDKR
                     YHQVLLAKNQQGYQNLIKLTSLGYIEGMYSKYPRIDKELILKHSEGLIATSCCIGAYI
                     PQTILNEGEEAAEKEFKWWLDVFGDDFYIELQRHNLKDQVLINDTLLKFSKKFLV"
     gene            complement(707..1906)
                     /locus_tag="PLU36_03365"
     CDS             complement(707..1906)
                     /locus_tag="PLU36_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HMZ45820.1"
                     /translation="MVSFSTLYNNLKPIILIGFICIYSTKTKAKQLEKKDTVAPIVKS
                     KLIFIKAETIKTAAGTKSVVFNTNGTKLYAMNLEGMSISEFDRATRINTRVFKFKGTK
                     ALGWDYKKHTTMDSFEEKPVEACFSNNNQILWVSLHNAKGIVPINFSKDSIHHDLNLK
                     KKKIYIQYPDKKRKDSLEVPLIKTGKTPKVIACTSNDSFLLVSNWHSNNVSVVKLNNG
                     VYPYGKVIATIPVQSIPRGIAIDNDNQKTYIAIMGSKLISVIDNKTWLFERNIKVASK
                     PRHIVLDNKKRLFVSYNSLDKIACIDALSGKKLFDIKTKNQPRTIALSKNNKYLFVTC
                     YGGNALEVFKINDTGFIKLYTLESEGKPVGVDIYENENTIEAWVCNYKEGNVKIFTFK
                     KEEILKK"
     gene            2151..2903
                     /locus_tag="PLU36_03370"
     CDS             2151..2903
                     /locus_tag="PLU36_03370"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HMZ45821.1"
                     /translation="MKIQNTFYILAAFAILTSCKSLHTLTAKDVSSMPKPTASKQQHK
                     VTFIENIEVAPGTVVTSKHKTINTTSKNKSEEKKSTQTVTYTKPKVEYTESTIENADY
                     LQLKYAVILDATVEKLNNLHLLQVIDKWWGTKYCLGGSTEQCIDCSAFTQTIAKEVYE
                     TVLPRTSREQYEVVEKIETDDLKEGDLVFFHSGGRKKRVSHVGFYIMNNKFVHASTSN
                     GVMISDLDESYWKQKYKGAGRLRKQNSIVSKN"
     gene            complement(2911..4656)
                     /locus_tag="PLU36_03375"
     CDS             complement(2911..4656)
                     /locus_tag="PLU36_03375"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HMZ45822.1"
                     /translation="MTLLLRRTLVLTVMLLSSFSVLYVNNTSAQILNKKESFNADDSL
                     RGTLNANRNWWNVLRYDITVKPNIVTKEITGIVLIQAIATASGKTMQLDLQQPLIVDS
                     IVSKGNYFTVKNGVKKTLTSIHEQYKFIQTGNIVLVDLNTIQRKGNRINFHIYYHGKP
                     KEAIKPPWDGGWIWKKDKQGNPFVSVAVQGLGASAWFPCKDHQSDEPDLGATLKITVP
                     DTLVAVGNGKIIAIDKVPNSNLNTYTWQVKNPINSYNIIPYIGKYAVINDTLKGKKGT
                     LNLSYWVLDYNTDKALKQFEQVKLMLRAFEYWFGPYPFYEDGYKIVEAPHLGMEHQSA
                     IAYGNNYKNGYLGTDRSGNTGWGLKWDFIIIHESGHEWFGNNITAKDVADMWIHEAFT
                     TYSETLFTEYYYGKQAANEYNYGQRKNIRNDVPIIGQYGVNKEGSSDMYDKGSNMLHS
                     IRHAINNDELFRKILRGLNKTFYHKTVTSQQIEDYISKNARFDFSTVFDQYLRATQIP
                     ILNYKISNDGKTVTLKYSNCVVGFNLPIWLNNNAQRINFNNNSESQIINADENILTEI
                     QNLDKLYYIQIKEEN"
     gene            4721..6346
                     /locus_tag="PLU36_03380"
     CDS             4721..6346
                     /locus_tag="PLU36_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMZ45823.1"
                     /translation="MKWLIKIKLIWLFFYISFLNYGCTQNNNSSKKIFSYNETAGIAT
                     LDPAFAKNQSIMWAVHQLYNTLVEVDSNLQIVPSLAKSWSISEDKRTYTFYLRNNIFF
                     HDNQAFKNGKGRKMNANDIVYSFQRIINKKTASSGAWIFNNKVDTIEPYKAINDTTFQ
                     LKLLQPFHPILGILSMQYCSIVPKEVVEKFGKDFRNNPCGTGPFKFFMWQEGQALVLH
                     KNESYWEKDLDGTPLPKIDAIKVSFFDNKATEFLQFRQGKLSFINDIDASFKDEVLTK
                     NGLLRKNWQNKIKLNKHAYLNTEYLGILMDENNELVKSSPLKIKAIRKAINYAINKEQ
                     LMLYMRNSIGFAANNGFIPYGLPANKLQDKFFEYNPQKAKKILDSIQYNYTTIPITLL
                     TIPVYADVASFITKQCELVGIKMQVEVIQKSLLLEQTAKSKAMFFRGSWIADYPDEEN
                     YFAVFYSKNPAPPNYTRFNNKTYDKLYEQALQETNDSARLNLYKALDNIIKEELPIIP
                     IWYDMIIHLVNNNVQNFTPNSLNLLELRRVVIE"
     gene            complement(6351..6764)
                     /locus_tag="PLU36_03385"
     CDS             complement(6351..6764)
                     /locus_tag="PLU36_03385"
                     /inference="COORDINATES: protein motif:HMM:NF025442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HMZ45824.1"
                     /translation="MKKAKPFIALVILVVVAIFLRNCVSNKSNKKEVDENVKQRGLNR
                     NPSNINYTKHAKCRMDCRHIDEKEVVYILENGKINYSKSDLKGKDCNKKYAVEGISKD
                     KQKLRIVFAPCNNEVTVITCIDLDTEWECSCPGDE"
     gene            complement(6812..7435)
                     /locus_tag="PLU36_03390"
     CDS             complement(6812..7435)
                     /locus_tag="PLU36_03390"
                     /inference="COORDINATES: protein motif:HMM:NF021759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HMZ45825.1"
                     /translation="MPIVEKPLNSLAHFLPDESLDIVLQFIQQYKIHLLVTQKRKTIL
                     GNYKHAGLQSNHKISVNGNLNKYEFLITFLHELAHLLTYEQFKNKVEPHGKEWKFFYS
                     KLLTEFVQLKIFPNDIVIALQKSILNPAATANGETDLLRVLRKYDTANNPQLQMIENI
                     PTHSKFSIENGRIFIKGQKRRKRIECIEISTNKIYLFNPLVEVKLFE"
     gene            complement(7435..7842)
                     /locus_tag="PLU36_03395"
     CDS             complement(7435..7842)
                     /locus_tag="PLU36_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45826.1"
                     /translation="MFQQSKFGKLIMPFFALFTLVNIVFITWAEKWDAIKLNHTVIIF
                     GNALLFLLSIFSIWMHLKAVNHKNPSVMVRGVLGSTFVKIILIATSILLYLYFTPKEH
                     QSKLAVIAVMVLYLIYVFIEVRVVVRLNKKKVK"
     gene            complement(7842..8045)
                     /locus_tag="PLU36_03400"
     CDS             complement(7842..8045)
                     /locus_tag="PLU36_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45827.1"
                     /translation="MYNKKNNILIKYAGMATQLLVGLGLTTWFGNWIDKKNIFKKPVF
                     TWILPLIFVIGFLIKLIKDLNKK"
     gene            8175..9806
                     /gene="pruA"
                     /locus_tag="PLU36_03405"
     CDS             8175..9806
                     /gene="pruA"
                     /locus_tag="PLU36_03405"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HMZ45828.1"
                     /translation="MSLGTFSYPMPVNEPVLNYAPNSAEKIKLKATLAELKNTEIDVP
                     MYIGNKEVRTNRKVAIRPPHEIKHILGYFHAGEEKHVHQAIEAALAAKESWANMQWET
                     RAAIFLKAADLIATKYRSYINGTTMLGQSKNAYQAEIDSACELIDFLRFNVHFLSEIY
                     KQQPISGPGMHNRTEWRPLEGFVLAVTPFNFTAIGGNLPTSAAMCGNVVVWKPANTQI
                     YSAQMFMKILKEAGLPDGVINLIYVDGPTIGKVCFNHHEFAGVHFTGSTGVFNNMWET
                     IGKNMAKYKSYPRIVGETGGKDFVLAHKSANADVVATAILRGAFEFQGQKCSAASRAY
                     IPSNIADEVKKKLIAGVKSMKMGTTEDFTNFINAVIDEKSFDNIKRYIDNAKKDAKTK
                     ILVGGKCDKKEGYFIQPTVIETTNPKYTTMCEEIFGPVLTLYVYPANNFEKALELVDS
                     TSPYALTGAILAQDKAAIELATNKLRNAAGNFYINDKPTGAVVGQQPFGGARASGTND
                     KAGSALNLYRWLSARTIKETYNPPTDYSYPFLQEA"
     gene            9987..12326
                     /locus_tag="PLU36_03410"
     CDS             9987..12326
                     /locus_tag="PLU36_03410"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HMZ45829.1"
                     /translation="MSNKDIRREQALEYHAQGKPGKIEVIPTKEAKTQRDLSLAYSPG
                     VAEPCKEIAKDIDNVYKYTAKGNLVAVISNGTAVLGLGDIGPEAGKPVMEGKGVLFKV
                     FADIDVFDIEINEKDPVKFVEIVKALEPTFGGINLEDIKAPECFYIEKELKKQLKIPV
                     MHDDQHGTAIISAAALLNALELQKKKIEKVKVVVNGAGAAAISCIKLYVALGVKKENV
                     KVFDSKGLITKQRTDLDEQKLEFAIDCEDMTLAEGIKNSDVFIGLSKGNVVSKEMILS
                     MAKHPIVFAMANPDPEISYEDATDARNDVIMATGRSDFPNQVNNVLGFPYIFRGALDV
                     RATTINEEMKLAAVRALADMAKTPVPDIVNLAYNLKNLSFGTDYIIPKPVDPRLISIV
                     APAVAKAAIESGVAKKTITDWKAYELELNKRLGLDNQLLRAIGNKARRDPKRLVFAEA
                     DNDKILKAASIIYDEGIAYPILLGTEKKIREIANEHNIDLTDIPIIDPRDEQYESKRE
                     LYGELFFAKRQRKGFNHYESIKIMKERNHFGCMMVETGDADAMISGLKRNYAEAIRPA
                     LQIIGTEEGVQKIAGMYLLSTQKGPIFLADTTINYNPTAEELADIALLVAKEVRSFNI
                     TPRIAMLSYSNFGSSNSHEARLVAEATSILKQKNPSLIVDGEMQGSLAFNKEVLKENY
                     PFSNLIDENVNVLIFPNLTAGNIAYNLLKELGGADAIGPILLGLKKPVHVLQLGSSIR
                     NIINVANIAVIDAQVKNKLDTDTEVQRTNWWNRLKKKNK"
     gene            12345..>12721
                     /locus_tag="PLU36_03415"
     CDS             12345..>12721
                     /locus_tag="PLU36_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ45830.1"
                     /translation="MKFFSHFGRYILMLKSMFTKPENHKMLWKEFMHQSVEIGIGALP
                     IVIIISLFLGAVTTVQTAYQLVSPLVPQSTIAQIVRDSVILELSPTVVSIVLAGVVGS
                     KIASELGNMQVTEQIDALEIMGI"
BASE COUNT         4534 a         1909 c         1976 g         4302 t
ORIGIN      
        1 acattagctc tataatgttc tgctaactcg tcaatttata ctaaaaattt tttactaaat
       61 tttaaaagag tatcgttaat aagtacttga tcttttaaat tatgcctttg taattcaata
      121 taaaaatcat ctccaaacac atctaaccac cacttaaatt ctttttcagc agcctcctct
      181 ccttcgttta atattgtttg tggaatgtat gctcctatac aacaagaagt agcaattaaa
      241 ccttcagaat gttttaaaat aagttcttta tctattctag gatatttgct atacatacct
      301 tctatatatc ctaaggaggt aagttttata aggttttgat aaccttgttg attttttgcc
      361 agtaatactt gatggtaccg tttgtctttt gctcctttcg taaaggaacg tatatgccta
      421 ttttctacaa cataaaactc gcagccaact attggtttta caattggttc aagtttttct
      481 tttccatctt ctgttttgcc tacaactttt gtttttttcc atgcttctgc tacaaaagaa
      541 aatgcaccaa acatattgcc atgatctgtt attgcaactg caggcatatt atctcttagt
      601 gcttttttat acaagctttc aatagaggca gcaccatcta acaaagaata ttgtgtgtgt
      661 gtatgtaagt gtgaaaattg catttttata atataattct ccttaattat ttttttaaaa
      721 tttcttcttt tttaaaagtg aatattttta cgtttccttc tttgtaatta catacccaag
      781 cttctattgt gttttcattt tcataaatat ctactccaac aggtttgcct tcactttcta
      841 aagtatataa ttttataaag cctgtatcgt ttattttaaa aacttctaat gcattaccac
      901 cataacaggt aacaaacaaa tatttattgt ttttacttaa tgcaattgtt cttggttgat
      961 ttttagtttt tatatcaaaa agttttttac cacttaatgc atctatacat gcaattttat
     1021 ctagactgtt atagctaaca aaaagtcttt ttttattgtc taatacaata tgccttggtt
     1081 tagaagcaac cttaatattt cgttcaaata accaggtttt attatctata acacttatta
     1141 acttactccc cattatagca atatacgttt tttggttatc attatctata gcaatacctc
     1201 ttggaattga ttgtacagga attgtagcta ttaccttacc ataaggataa acaccattgt
     1261 ttaattttac tacactaaca ttgttgctat gccaattact aaccaataaa aaactatcat
     1321 tactggtaca agcaattacc ttgggtgttt taccagtttt aattagtgga acttccaaac
     1381 tatctttcct ttttttatct ggatattgta tataaatttt tttctttttt aaattcaaat
     1441 catggtgaat agaatctttt gaaaaattta taggaacaat tcctttagca ttatgtaagg
     1501 atacccatag aatttgatta ttgttactaa aacatgcttc taccggtttt tcttcaaagg
     1561 aatccattgt agtatgtttt ttgtaatccc agcccaaggc ttttgttcct ttaaacttaa
     1621 atactcttgt attaattctt gttgctctgt caaattcaga aatactcata ccttctaaat
     1681 tcattgcata aagtttagtg ccatttgtat tgaatacaac tgattttgta cctgctgctg
     1741 ttttaattgt ttctgctttt ataaatatga gttttgattt aactattggt gcaactgtat
     1801 cctttttctc tagttgtttt gctttagttt ttgtgctata tatacaaata aatccaatta
     1861 aaatgattgg tttaaggtta ttatatagtg tagaaaatga aaccattgta gctctgttaa
     1921 cttaattttc aaatatcgtt tttaattaag atggttaaaa atatcaatta tccacaataa
     1981 ggttaaactg aaaagaatgt taaaaagttt tgcagaatga tgttttttta ggtaatgaag
     2041 ggttaatttg catcaatcgt gtactaatta tttgcttttt gtatttaatt attaaattaa
     2101 ataagttgta attatatttt ttacaccatg taggaaacaa aagaggatac atgaagatac
     2161 aaaacacatt ttatatattg gcagcatttg ccattttaac cagttgtaaa tctttgcata
     2221 ctttaactgc taaagatgtt tcatcaatgc ctaagcctac agcctctaaa caacaacaca
     2281 aggttacttt tattgaaaat attgaggttg ctcctggaac tgttgtaacc tccaaacata
     2341 aaactattaa taccacttca aagaataaat cagaagaaaa gaaaagcaca caaactgtta
     2401 cttacactaa accaaaagta gaatacacag aatctacaat tgaaaatgca gattatttac
     2461 agttaaaata tgctgtaatt ttagatgcaa ctgtagaaaa gctaaacaac ctgcatttat
     2521 tacaagttat agataaatgg tggggcacta aatattgcct tggtggctct actgagcaat
     2581 gtattgactg ttctgcattt acccaaacca ttgcaaaaga agtttatgaa actgttttac
     2641 ctagaacatc tagagagcaa tacgaagtgg tggaaaaaat tgaaacggat gatttaaaag
     2701 aaggtgattt agttttcttt catagtggag gaagaaaaaa gagagtttcg catgttgggt
     2761 tttatataat gaataataag tttgtacatg catctaccag caatggagtt atgattagtg
     2821 atttagacga atcttattgg aaacaaaaat ataaaggcgc cggcagatta agaaagcaaa
     2881 attctattgt aagtaaaaat tagccttcta tcaattctct tcttttattt gtatataata
     2941 cagcttatct aaattttgta tttcggttaa aatattttca tctgcattta ttatttggct
     3001 ttcgctgttg ttattaaaat ttattctctg agcattattg tttaaccaaa ttggtaagtt
     3061 aaaccctaca acacaattgg aatattttaa agttactgtt ttaccatcgt tgctaatttt
     3121 ataatttaaa ataggtattt gagtagctct taaatattgg tcaaaaactg ttgaaaaatc
     3181 gaatctagca tttttagaaa tatagtcttc aatttgttgt gatgtgactg ttttatgata
     3241 aaaagtttta tttaaccctc ttaaaatttt tctaaacaac tcatcattat taatagcatg
     3301 acgaatagag tgtaacatat tactgccttt atcatacata tcactacttc cttctttatt
     3361 aacaccatat tgcccaatta ttggtacatc atttcttata ttttttcttt ggccataatt
     3421 gtattcattg gctgcttgct taccataata atattcagta aataatgttt cgctgtaggt
     3481 tgtaaatgct tcgtgaatcc acatatcagc aacatctttg gcagtaatat tgttgccaaa
     3541 ccattcatgt ccactttcgt gaattattat aaaatcccat tttaagcccc aacctgtgtt
     3601 accacttctg tcagtaccta aataaccatt tttgtaatta ttaccataag caattgcact
     3661 ttgatgctcc atacctaaat gaggtgcttc tacaattttg tagccatctt cgtaaaaagg
     3721 ataaggacca aaccaatact caaaagccct aagcataagc tttacctgtt caaattgttt
     3781 taatgcctta tctgtattat aatctaaaac ccagtagctt aaatttaatg ttcctttttt
     3841 cccttttaaa gtatcattaa taacagcata tttaccaatg tatggaataa tgttgtaact
     3901 gttaattggg ttttttactt gccaagtgta agtatttaaa ttagaattgg gcactttatc
     3961 tattgcaata attttaccat tgccaacagc aactaaagta tctggcacag taatttttaa
     4021 agtagctcct aaatctggtt catcgctttg atggtctttg caaggaaacc atgcacttgc
     4081 tcctaagcct tgtacagcaa cacttacaaa agggttgcct tgtttgtctt tcttccaaat
     4141 ccaacctcca tcccaaggtg gttttatggc ttctttgggc ttgccatgat agtaaatgtg
     4201 aaaattgatt ctatttcctt ttctttgaat tgtatttaaa tcaactaaaa caatgttgcc
     4261 tgtttgtata aatttatact gctcatgtat agaggtaagg gtttttttaa ctccattttt
     4321 tactgtaaaa taatttcctt ttgatacaat gctatcaact attaaaggct gttgtaaatc
     4381 taattgcatt gttttgccag atgcagtggc tattgcttga attaaaacaa ttcctgtaat
     4441 ttctttagtt actatattgg gctttacagt aatatcatat cgcaatacat tccaccaatt
     4501 tctgtttgca tttaatgtgc ctcttaaact atcgtcagca ttaaaacttt cttttttgtt
     4561 taaaatttgt gccgaagtat tatttacata tagtacacta aaactgctca gtaacattac
     4621 agtaagcaca agggtgcgac gcaacaaaag tgtcatagca agtataaagt ttgttaaata
     4681 aaaatatctt taaaacatta aaagtaagat attcgttgta atgaaatggc ttattaaaat
     4741 aaaattaatt tggttgtttt tttatatcag ctttttaaac tatggttgta cacaaaataa
     4801 caatagtagt aaaaaaatat ttagttataa cgaaactgca ggcattgcaa ctttagaccc
     4861 tgcatttgca aaaaatcaaa gtattatgtg ggctgtacat cagttgtata atacattggt
     4921 tgaagtggat agcaacttgc aaattgtacc atccttagca aaaagttgga gcattagtga
     4981 ggataagaga acatacactt tttatttaag aaacaatatt ttttttcatg acaaccaagc
     5041 atttaaaaat ggtaaaggaa gaaaaatgaa tgccaatgat attgtctata gttttcagag
     5101 aattattaat aaaaaaactg caagtagtgg ggcatggatt ttcaataata aagtagatac
     5161 tattgaacca tataaagcta ttaacgatac tacttttcaa ctaaaactat tacagccttt
     5221 tcatcctatt ttgggtattt taagcatgca atattgtagt attgtaccaa aggaagtggt
     5281 agagaaattt ggtaaagatt ttagaaataa cccttgtggc acagggccat ttaaattttt
     5341 tatgtggcaa gaggggcaag cattggtatt gcacaaaaat gagagctatt gggagaaaga
     5401 tttggatggc acaccattac ccaaaataga tgcaattaaa gtaagttttt ttgataataa
     5461 ggcaacagaa tttttacaat ttaggcaagg taaattaagt tttataaatg atatagatgc
     5521 tagttttaaa gatgaggttt taaccaaaaa cgggctactg agaaaaaatt ggcagaacaa
     5581 aattaaactt aacaaacatg cctatttgaa tactgaatat ttaggaatat taatggatga
     5641 aaataatgaa ctggtaaaaa gctctccatt aaaaattaaa gcaatcagaa aagctattaa
     5701 ttatgccatt aacaaagagc aattaatgct gtacatgcgt aacagcattg gctttgctgc
     5761 caataatggt tttatacctt atggtttacc tgctaacaaa ttacaggata agttttttga
     5821 atataaccct caaaaagcaa aaaaaatatt agatagcatt cagtacaatt atacaactat
     5881 acctattacg ttattaacta tacctgtata tgcagatgtt gcaagtttta ttacaaagca
     5941 atgtgaattg gttgggataa aaatgcaagt ggaagtgatt caaaaaagtt tattgttaga
     6001 acaaactgct aaaagtaaag ccatgttttt tagaggtagt tggattgccg attaccctga
     6061 tgaagaaaat tattttgcag tattttatag taagaaccct gcaccaccca actatactag
     6121 atttaacaat aaaacttatg ataaattata cgaacaagca ttgcaagaaa ctaatgatag
     6181 tgcaagatta aatttataca aggcattgga taatattata aaagaagaat taccaattat
     6241 accaatatgg tacgatatga ttatacattt ggtaaacaat aatgtacaaa atttcacccc
     6301 taattcttta aacttgctag aattgagaag ggttgttata gaataattat ttactcatca
     6361 ccagggcagc tacactccca ttctgtatct aaatcaatac aggttataac agtaacttcg
     6421 ttgttgcatg gggcaaaaac tattctcaat ttttgtttgt ctttactaat accttctaca
     6481 gcatattttt tattgcaatc tttacctttt aaatcacttt tgctgtaatt aatttttcca
     6541 ttttctaaaa tatacactac ttctttttca tcaatatgcc tacaatccat tctgcatttt
     6601 gcatgtttag tgtaatttat attagatgga tttcggttta aacctctttg ttttacattt
     6661 tcatctacct ctttcttgtt agatttattg cttacacagt ttcttaaaaa aatagcgact
     6721 accactaaaa taacaagggc aataaatggc ttagctttct tcataacaaa aagtttaaag
     6781 taagtgcaaa atacaaataa ctgtgctata attactcaaa cagttttact tctaccaaag
     6841 gattaaataa ataaattttg ttggtgctta tttctataca ttctatcctt tttcttcttt
     6901 tttgcccttt aataaaaatc cttccattct caatactaaa tttagaatga gttggaatat
     6961 tttcaatcat ttgtaattga gggttattag cagtatcgta ttttcttaaa acacgtagta
     7021 aatctgtttc gccattagca gtagcagcag gatttaaaat agatttttga agagctataa
     7081 ctatatcgtt tgggaaaatt tttagttgaa caaactcagt taatagttta ctataaaaaa
     7141 acttccactc ttttccatga ggctctactt tatttttaaa ttgttcgtag gtaagcaaat
     7201 gggctaattc atgtaaaaaa gtaattaaaa actcatactt attcaaatta ccattaaccg
     7261 aaattttatg attgctttgt aagcctgcat gtttatagtt tcctaaaatg gtttttcttt
     7321 tttgagtaac aagcaaatga attttgtatt gttgaataaa ttgaagaaca atatccaaac
     7381 tttcatcagg taaaaaatga gctaaactat ttaaaggttt ctcaactatt ggcattactt
     7441 aactttcttt ttatttagcc ttacaactac cctaacttca ataaatacat aaatgagata
     7501 taaaaccatt actgcaataa ctgctaattt gctttgatgt tctttaggag taaaatacaa
     7561 atacaataaa atagaagtgg ctattaaaat tatttttaca aaagtgctgc ctaaaactcc
     7621 tcttaccatt actgatgggt ttttatgatt tacagctttt aaatgcatcc aaatactaaa
     7681 tatggaaagt aaaaacagca atgcattccc aaaaataata acagtatgat taagtttaat
     7741 ggcatcccat ttttctgccc aagtaataaa tacaatattc actaaagtaa acaaggcaaa
     7801 aaatggcatt attaatttgc caaatttaga ttgctgaaac attatttttt atttaagtct
     7861 tttattaatt ttattaaaaa tccaattaca aatattaaag gtaaaatcca ggtaaaaaca
     7921 ggctttttga atatattttt tttatctatc cagttgccaa accatgtagt taacccaagc
     7981 ccaactaata gttgtgttgc catacctgcg tacttaatta atatattgtt ttttttattg
     8041 tacatatttt aaaatacaat gataagctat taattatatt ttaaaaaaca gatgaatttc
     8101 aactaaatct cttttaaaga agatgagctg aaaacttcct taactttgca gccttaaatc
     8161 taaaattttt cattatgagt cttggaacct tttcttaccc aatgcctgta aatgagccag
     8221 ttttaaacta tgcccccaac agtgcagaaa aaataaaact taaagccact ttagccgaat
     8281 taaaaaatac tgaaatagat gttccaatgt atattggaaa taaagaagta agaaccaata
     8341 gaaaagtagc cattcggcca ccacatgaaa taaaacacat attaggatat tttcatgcag
     8401 gcgaagaaaa acatgtacac caagcaattg aagcagcatt agctgctaaa gaaagttggg
     8461 caaatatgca atgggaaacc agagcagcca tttttttgaa agctgccgat ttaattgcta
     8521 ccaaatatcg tagctacata aatggcacca ccatgcttgg ccaaagtaaa aatgcctacc
     8581 aagcagaaat tgatagtgca tgtgaattaa tagatttttt gagattcaat gttcactttt
     8641 taagtgaaat ttataagcaa caacctatta gtgggcctgg tatgcacaac agaactgaat
     8701 ggagaccatt agaaggattt gttttagctg taacaccatt taattttaca gccattggtg
     8761 gtaatttacc tactagtgca gccatgtgtg gcaatgttgt agtttggaag ccagccaata
     8821 cgcaaattta ttctgcacaa atgtttatga aaattttaaa agaagcagga ttaccagatg
     8881 gtgttattaa tttaatttat gtagatgggc caaccattgg aaaagtgtgt tttaatcatc
     8941 atgaatttgc aggtgtgcat tttacaggaa gtacaggtgt atttaataat atgtgggaaa
     9001 ccatcggaaa aaatatggca aaatacaaat cctatccaag aattgttgga gaaacaggag
     9061 gaaaagattt tgtgttggcc cataaatctg ccaatgccga tgtagtggct actgccattt
     9121 tacgtggtgc atttgaattt caggggcaaa aatgtagtgc agcttccaga gcatatatac
     9181 caagtaatat tgcagatgaa gtgaaaaaga aattaattgc aggtgttaaa agcatgaaaa
     9241 tgggaacaac agaagacttc accaatttta ttaatgccgt aatagatgag aaaagttttg
     9301 ataacattaa gcgatatatt gacaatgcta aaaaagatgc aaaaactaaa atcttagttg
     9361 gcggtaaatg cgataaaaaa gaaggttatt tcattcaacc tacagtaatt gaaactacca
     9421 atccaaaata tacaactatg tgtgaagaaa tttttggccc tgttcttaca ctatacgtat
     9481 atcctgcaaa taattttgaa aaagccttag aattagtaga tagtacaagc ccttatgctt
     9541 taacaggtgc tatactagca caagataaag ctgctattga gcttgcaaca aataaattaa
     9601 gaaatgctgc cggaaatttt tatataaatg ataaaccaac aggtgctgtt gttggtcaac
     9661 aaccttttgg tggtgcaaga gccagtggta ctaacgataa agcaggcagt gctttaaatt
     9721 tatatcgttg gttaagtgca agaacaatta aagaaactta taacccacca actgattata
     9781 gttacccttt tttacaagaa gcataaaaat aacaattgtt caaaaaataa agtcgtttag
     9841 gatgtttatc attttaaacg acttttttta tcctgaattt caaaaaaagc agtattcaaa
     9901 ttaactactt tattgtattt tgcactattt cactttaagc ttaaaaacaa ttttatttct
     9961 attccaatca ataaagtata aaacctatga gcaataaaga tataagaaga gaacaagcat
    10021 tagaatacca tgcacaagga aaaccaggca agattgaagt aattcctaca aaggaagcaa
    10081 agacacaacg agacctttca ttggcatata gcccaggtgt agcagagcca tgtaaagaaa
    10141 ttgcaaaaga tattgataat gtttacaaat acacagcaaa aggtaattta gttgctgtaa
    10201 taagcaatgg cacagcagtt ttaggcttag gagatatagg ccctgaagct ggcaaaccag
    10261 ttatggaagg aaaaggtgtt ttatttaaag tatttgccga tattgatgtt tttgatattg
    10321 aaattaatga aaaagaccct gtaaagtttg ttgaaattgt aaaagcatta gaacccacat
    10381 ttggaggtat aaacttagag gatattaaag cacctgaatg tttttatatt gaaaaggaat
    10441 taaaaaaaca attgaaaatt cctgtaatgc atgatgacca acatggcact gctataataa
    10501 gtgcagctgc tttactaaat gcattagaac tgcaaaaaaa gaaaatagaa aaagtaaaag
    10561 tagtggttaa tggtgctgga gcggcagcca tttcttgtat aaaactctat gttgcacttg
    10621 gagttaaaaa agaaaatgta aaagtatttg atagcaaagg attaataact aaacaacgaa
    10681 cagatttaga tgaacaaaaa ttagaatttg caatagattg tgaggatatg acattggcag
    10741 aaggaataaa aaatagcgat gtttttattg gattaagtaa aggcaatgta gtaagcaaag
    10801 aaatgatatt aagcatggca aaacatccaa ttgtttttgc aatggctaat cctgatcctg
    10861 aaattagtta tgaagatgca acagatgcaa gaaatgatgt tattatggca accggtagaa
    10921 gcgattttcc taatcaggtg aataatgttt tagggtttcc atacattttt agaggagctt
    10981 tagatgttag ggcaacaacc attaacgaag aaatgaaatt agcagccgta agagcattgg
    11041 cagatatggc aaaaacacct gttccagata ttgtaaacct tgcctataat ttaaaaaatt
    11101 tatcttttgg aactgattat attattccaa aaccagttga cccaagattg atttctattg
    11161 tagcaccagc agtagcaaaa gctgcgatag aaagtggtgt agctaaaaaa actattaccg
    11221 attggaaagc ttatgaactt gaactaaata aaagattagg cctagataac caattattaa
    11281 gagcaattgg taacaaagca agaagagatc caaaacgctt ggtatttgcc gaagccgata
    11341 acgataaaat tttaaaagca gcaagtatta tttatgatga aggaattgcc taccctattt
    11401 tattaggaac agaaaagaaa ataagagaaa tagccaatga acataatatt gatttaaccg
    11461 atattccaat tattgaccct agagatgaac aatacgaaag caaaagagaa ctttatggcg
    11521 aattgttttt tgcaaaacgt caacgcaaag gctttaacca ctacgaaagt attaagataa
    11581 tgaaagaaag aaaccacttt ggttgtatga tggtagaaac aggagatgcc gatgcaatga
    11641 taagtggttt aaaaagaaat tatgccgaag ccatcagacc tgcattacaa attattggta
    11701 cagaagaagg tgtacaaaaa attgcaggca tgtatttact aagtacacaa aaaggcccaa
    11761 tatttttggc agataccact attaattata acccaactgc agaagaactg gcagatattg
    11821 ctttattagt tgctaaagaa gtaagaagtt ttaatattac accacgtatt gctatgttaa
    11881 gctatagtaa ttttggtagt agcaatagcc acgaagcaag attagttgca gaagcaacca
    11941 gcatacttaa acaaaaaaat ccatccttaa ttgtagatgg cgaaatgcaa ggcagtcttg
    12001 cattcaacaa agaagtgcta aaagaaaatt atccattcag taatttgatt gacgaaaatg
    12061 taaatgtttt aattttccct aacctaacag caggtaatat cgcttataat ttattaaaag
    12121 aattaggtgg agcagatgca attggtccta ttttattggg gttaaaaaaa cctgttcatg
    12181 ttttacaatt aggtagcagt ataagaaata taattaatgt agctaatatt gcagtaatag
    12241 atgcacaagt aaaaaacaaa ttagatacag acacagaagt tcaaagaacc aactggtgga
    12301 acagattgaa aaaaaagaat aaataaaaaa cagattacaa tacaatgaaa tttttttcgc
    12361 attttggcag atacatttta atgctcaaaa gcatgtttac taagccagaa aatcataaaa
    12421 tgctctggaa ggaattcatg catcaaagtg ttgaaattgg cattggtgca ttacctattg
    12481 taattatcat ttctttattt ttaggagctg taacaactgt gcaaacagct taccaattgg
    12541 ttagcccctt agttccacaa agtactattg cacaaattgt aagagatagc gttatacttg
    12601 aactttcgcc gactgttgta agtattgtat tagctggtgt ggtaggtagt aaaattgcaa
    12661 gtgaattagg aaatatgcaa gtaacagaac aaatagatgc tttagaaata atgggaatta
    12721 a
//
LOCUS       DAOQPK010000042        12680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_2549_length_12680_cov_4.046178, whole genome shotgun sequence.
ACCESSION   DAOQPK010000042 DAOQPK010000000
VERSION     DAOQPK010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12680
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2549_length_12680_cov_4.046178"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PLU36_03420"
     CDS             <1..574
                     /locus_tag="PLU36_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HMZ45831.1"
                     /translation="AKHIPAAIVNAGDGINEHPTQALLDAFSMQEKFGSLEGLKVAIV
                     GDIMHSRVALSNIYLLQKMGAEVVVTGPPTLIPKYITEALGVKVEYNIKNALQWCNVA
                     NVLRIQLERQNQPLFSSLREYNLSYGISKKLIDSLNKEITIMHPGPINRGVELDSDIA
                     DGSSSIILNQVENGVAVRMAVLYLLANREN"
     gene            609..1082
                     /gene="coaD"
                     /locus_tag="PLU36_03425"
     CDS             609..1082
                     /gene="coaD"
                     /locus_tag="PLU36_03425"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HMZ45832.1"
                     /translation="MSSRICLFPGTFDPVTLGHVDIINRALPLFDKIYIGIGENAAKK
                     PMFTAEQRLVWFKDIFNNEPKIESVIFQGLTIEYCKKINARFILRGIRYVSDFEYEKT
                     IADANRTLDRTIETIFLTGEPKYTSVASTIVRDILKNGGDASPFLPEAVINSLKK"
     gene            1104..1538
                     /locus_tag="PLU36_03430"
     CDS             1104..1538
                     /locus_tag="PLU36_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HMZ45833.1"
                     /translation="MIWYNKQIAIKDFAHWGKNTMGEFLDIQFIEIGADFLKATMPVN
                     TKTHQPYGLLHGGASATLAETVGSVASALVINTEKQLCVGLEINANHIRSVKSGIVTA
                     TAKPLHLGATTHVWDIKITDENEKLICVSRLTVAILNKKITH"
     gene            complement(1528..2625)
                     /gene="ychF"
                     /locus_tag="PLU36_03435"
     CDS             complement(1528..2625)
                     /gene="ychF"
                     /locus_tag="PLU36_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HMZ45834.1"
                     /translation="MSLKAGIVGLPNVGKSTLFNAVSNSAKAQASNYRFCTIEPNTGL
                     VDVPDYRLSKLAELVKPNRIVPTQLEIVDIAGLVRGASKGEGLGNKFLANIREVDAII
                     HVIRCFEDENVLREEGAINPVGDKEIIDIELQLKDLESVEKKIQRVEKLSRTDVKMKA
                     EYDVLMQCKLHLEKGNSIRSLGLSKEERVAISDLFLLTEKQVLYVANVDEASMHTGNK
                     YSEALKINAKAEGADVIVMCNNIEAQIAEMEAEEDKQMFMEEYGMKEPALDRLIQTTY
                     KILNLYTYFTAGVQEVRAWTIHQGWKAPQAASVIHTDFEKGFIKAEVIAYDDFVKYGN
                     EAAARENGRLRIEGKEYIVQDGDVMHFRFNV"
     gene            complement(2761..3192)
                     /locus_tag="PLU36_03440"
     CDS             complement(2761..3192)
                     /locus_tag="PLU36_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HMZ45835.1"
                     /translation="MKEKKKIIAAGGLVSNNNGDLLLIYRKKKWDLPKGKFEEGESIE
                     QCAIREVQEETGLQNVAITNFVGKTYHEYYEKWISQHVIKETWWYAMQITTNEPFLPQ
                     VEEDIEEVIWADENKINQCLTNSFSNIKSIIIQWKEQKNKS"
     gene            3316..3948
                     /gene="pyrE"
                     /locus_tag="PLU36_03445"
     CDS             3316..3948
                     /gene="pyrE"
                     /locus_tag="PLU36_03445"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HMZ45836.1"
                     /translation="MTNEKAVAEKLLQVQAIQLSPNAPFTWASGWKSPIYCDNRKVLS
                     FPFIRDFVKSEMCNIIFEKFEDIELLAGVATAGIAWGAMAADQLKLPYIYVRPKPKEH
                     GLGNQIEGFYEKGQKVLVIEDLISTGKSSMQVVNVLRNQGLEVVGIVSIFTYQFSEAV
                     QLFEENNLQHISLTNYTNLIDLAIEKQLVNAELQNVLLDWRDNPATWNGI"
     gene            complement(3960..4655)
                     /locus_tag="PLU36_03450"
     CDS             complement(3960..4655)
                     /locus_tag="PLU36_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ45837.1"
                     /translation="MAETVVQLRGVNIYQGNSLILQDVNFTIESGEFVYLVGKTGTGK
                     SSLLKTLYGELPLKEGSCNVVGFDLKDMNWKKVPFLRRNLGVVFQDFQLMTDRNVHEN
                     LKFVLKATGWKDEKLIEEKINDVLNKVGLRSKGFKMPYEMSGGEQQRVDIARALLNSP
                     KLILADEPTGNLDPETSDEIMQLLFQITKDYGTAIIMATHDFIVINRYPSRMLTTDRS
                     RVIDTTVAATMHS"
     gene            complement(4748..5755)
                     /gene="murB"
                     /locus_tag="PLU36_03455"
     CDS             complement(4748..5755)
                     /gene="murB"
                     /locus_tag="PLU36_03455"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HMZ45838.1"
                     /translation="MQIQYNLSLKPYNTFGINVIAKLFAAFNSVQQLIELLENNTDTT
                     LIIGGGSNILFTKNFDGLVLKNELKGIELICEDDEHYYIKVAAGEVWHQFVLYCIHNN
                     YNGIENLSLIPGNVGASPMQNIGAYGVEIKDTFYELEALHIQEKTIVKFNNSDCAFGY
                     RESIFKGKFKNQFIILNVTFKLHKKNIFNHSYGAIQQELDKMKVTALSAKAISDAVIS
                     IRSTKLPNPAIIGNAGSFFKNPVIAKTQYHKLLQQFPKLPGYNDKDDTIKIPAAWLIE
                     FCGWKGFRKNDVGCHTNQALVLVNYGNATGLEIYNLSQDIINDVYNKFSILLQREVNI
                     I"
     gene            complement(6031..6759)
                     /locus_tag="PLU36_03460"
     CDS             complement(6031..6759)
                     /locus_tag="PLU36_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ45839.1"
                     /translation="MAIKSIIIDDERLARTELKKLLQEHKDIEIIDEAANVDDGIMKI
                     ETLSPDLIFLDIQMPGKSGFDLLAEVEKAPRVIFTTAFDEYAIKAFEVNALDYLLKPI
                     EPKRLSDAIQKLQYEIQKEKSGLLNGRGPLTEEDQVFVKDGERCWFVKLHEIRLFESV
                     GNYAKVFFSTNKPLILKSLNALEERLDERTFFRANRKHIINLRWIEKIEPYFNGGLLV
                     ELKGGEKIEVSRRQTVKFKEMMSL"
     gene            complement(6797..7840)
                     /locus_tag="PLU36_03465"
     CDS             complement(6797..7840)
                     /locus_tag="PLU36_03465"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HMZ45840.1"
                     /translation="MKKKLVNYWLFQLGGWLLYALSMVFFSYLFRNNMDGIFWKRILL
                     FVFMGVAFTHVLRFCIHQFKLMPPIHGSKWINFFILVIFICIANSFVSSALIQYLGWY
                     RKEFNGTIGKRFIFTLFDSSPILLVWTSIYYVWHYVEDARRTEIDKIKLESVVKELEL
                     KTIKSHINPHFIFNALNSIRALVDENPIRAREAVTQLSNLLRSSMQTDKAETTSLEKE
                     LDIVKDYLALEHIRFEDRLQVEYEIDEDTLDQQIPPMMLQTLIENAIKHGIGKSKEGG
                     LIKIISRFIENHHELIVHNTGLIGNDYQQDGFGISSTKNRLALLFGKNASFNLTDDKI
                     TNKVIATIQLPIS"
     gene            8076..8393
                     /locus_tag="PLU36_03470"
     CDS             8076..8393
                     /locus_tag="PLU36_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HMZ45841.1"
                     /translation="MVVKIKEKSLWAKMAAYNMKASQMAIVFGNTIHLHNVTREEFLS
                     NRRWLRHEVAHVRQYQQKGFIRFILSYLFETFNMSYEFNKYEVEARKKETDLKVLEGV
                     EFI"
     gene            complement(8386..10494)
                     /gene="feoB"
                     /locus_tag="PLU36_03475"
     CDS             complement(8386..10494)
                     /gene="feoB"
                     /locus_tag="PLU36_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HMZ45842.1"
                     /translation="MQQNFNIVIVGNPNSGKSTLFNTLTGLNQKTGNFPGVTVDKKSG
                     KIELRNDIHANLIDLPGTYSLYPRRADEWVAYKVLMNADENIKPDVVLLVVDASNLKR
                     NLLFCSQILDTKIPVVIALTMNDLAAKKGIKIDIEGLQTDLGVPVVEVNPRKSKGTNE
                     LKKILLLLTKQAPVNNRDFIDNKTLAPQSIEAIQALQPNINTYTAIHYLINYQQLNVD
                     NSFKQQTATIIQSTNFNATKVQAEEVLQRYQRIRGLMQKNVFAPDPAEKKLITDKLDN
                     VLLHRKWGYLILLAVLFILFQSIFWLAQYPMNLIESSFASITSFFNHILPSTWYTDLF
                     INGIIAGLGGIVIFVPQIMILFGLITILEDTGYMARISFLSDKLMRKVGLNGKSVMPM
                     ISGLACAVPAIMSTRNIENRKERLLTILVTPLMSCSARLPVYTILIALAIPEKYYFGF
                     LSLQGLVMMALYLFGTFMALLVSFVMKWFIKINEKSFFILELPIYRNPRWKNVGLTMI
                     EKSKIFVVEAGKVIMVISLLLWFLSSYGPGDEMKKIDLHYEQLILQNPTQSDSLQKIA
                     STEKLEHSYAGLLGKKIEPIIKPIGYDWKIGIALITSFAAREVFVGTMATLYSVEESD
                     DSSLREKMQAATHRNGTKVYTLATALSLMMFYVLAMQCMSTLAIVKRETRTWKWPIFQ
                     FVYMTLLAYSMSWVVYEIFK"
     gene            10766..10838
                     /locus_tag="PLU36_03480"
     tRNA            10766..10838
                     /locus_tag="PLU36_03480"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10799..10801,aa:Lys,seq:ttt)
     gene            10937..11614
                     /locus_tag="PLU36_03485"
     CDS             10937..11614
                     /locus_tag="PLU36_03485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ45843.1"
                     /translation="MFLEKLLKSFVNKLGYELIKDKRKFITQGLTLPMHVFTNCKLLP
                     NRNAVLPFLPKQMVIAEVGVAYGEFSREIIDVTNPSKFYAIDYYQGEEFWGRNTFEKN
                     KLSHEDYIKQQFNKEIEQGIFYTKKGLSWDVLNTFSDNFFDYVYLDAGHDYESVKKDI
                     EVLLKKVKNGGVIQFNDYIIYDWVSKLPYGVVKAVDELLQNNQHEVLYYCLHKGGFND
                     IVVKINK"
     gene            11731..12147
                     /locus_tag="PLU36_03490"
     CDS             11731..12147
                     /locus_tag="PLU36_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HMZ45844.1"
                     /translation="MKKIHVVMLVCIAAAIAVLLGFMGNVTTYETIQSAKEKQGKIVT
                     VIAKLDTATLYYDPVKNPNYLTFEAIDSLGDRMKVAYYYEKPYDMEKSERVVLKGKME
                     NNVFEIRKKDGILVKCPSKYKDDMEAAQKNLDVTTK"
     gene            12195..>12680
                     /locus_tag="PLU36_03495"
     CDS             12195..>12680
                     /locus_tag="PLU36_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c assembly protein"
                     /protein_id="HMZ45845.1"
                     /translation="MKYIGEHLLPGQIGHFFIVLSLVASLVATFAFYFASKQNILEVK
                     KSWLKFARIAFLLESISVLAVFVLLYYIISHHLFEYKYAWQHSDKQLQLEYLLSCFWE
                     GQEGSFLLWSFWHCVLGWILIWKAKDWEAGVMTVVSFAQFCLATMTLGIYFFGHKVGS
                     NP"
BASE COUNT         4191 a         1926 c         1876 g         4687 t
ORIGIN      
        1 ggctaaacat atacctgcag ccattgtaaa tgcaggagat ggtataaacg aacatccaac
       61 acaagcttta ttagatgcat ttagtatgca agaaaaattt ggatccttag aagggctgaa
      121 agtggctatt gttggtgata ttatgcatag tagggttgca cttagtaata tttatttgtt
      181 acaaaaaatg ggagccgaag ttgtagtaac tgggccaccc actttaattc ctaaatatat
      241 cacagaagct ttgggagtaa aagttgaata caatattaaa aatgctttgc aatggtgtaa
      301 tgtggccaat gtattaagaa tacagttaga acgacaaaat cagcctctat tttcttcgtt
      361 gagagagtat aatttatcct atggaatttc aaaaaaactg atagacagtt taaataaaga
      421 aataacaatt atgcatcctg gtccaataaa tagaggggta gaattagata gtgatattgc
      481 agatggaagt tcctcaatca tcttaaatca ggtagaaaat ggggtagcag ttaggatggc
      541 tgttttatat ttgttagcca accgagaaaa ttaattatag agagttcaaa atttttatat
      601 gtaaacttat gagtagtaga atttgtcttt ttcctggaac atttgaccct gttacattag
      661 ggcatgttga tataattaat agagcattac cattgtttga taaaatttat attgggatag
      721 gagaaaatgc tgccaaaaag ccaatgttta ctgcagagca acgattggtt tggtttaaag
      781 atatttttaa taatgagcct aaaattgaga gtgttatttt tcaaggctta accattgaat
      841 attgtaaaaa aattaacgcc aggtttattt taagaggcat acgttatgta agcgattttg
      901 aatacgaaaa aaccattgcc gatgctaaca gaactttaga tagaacgata gaaaccatat
      961 ttttaactgg cgaacccaaa tatacttctg ttgcatctac tattgtaaga gatatactaa
     1021 aaaatggagg tgatgcatcg ccgtttttac ctgaagcagt tattaatagt ttaaaaaaat
     1081 aattctcttt tattttcatt gctatgattt ggtataataa acaaattgct attaaggatt
     1141 ttgcacattg gggtaagaat accatgggag aatttctaga tattcaattt attgaaattg
     1201 gtgccgactt tttgaaagct accatgcctg ttaatacaaa aacgcatcaa ccctatggct
     1261 tattacatgg tggtgcaagt gctacattgg ccgaaactgt tggtagcgtt gcctctgcct
     1321 tagtaattaa tacagaaaag caactatgtg taggcttaga aataaatgcc aatcatattc
     1381 gtagtgtaaa aagtggtata gttactgcaa cagcaaagcc cttgcattta ggggcaacta
     1441 ctcatgtatg ggatataaaa attaccgatg aaaatgaaaa attaatttgt gttagtaggc
     1501 ttactgttgc catactaaat aaaaaaatta cacattaaat ctaaagtgca ttacatcgcc
     1561 atcttgtaca atatattctt ttccttcaat tcttaatctg ccattttctc tagcagcagc
     1621 ttcgttgcca tattttacaa aatcatcgta agcaataact tctgctttaa tgaaaccttt
     1681 ttcaaaatcg gtatgtatta cacttgctgc ttgtggggct ttccaccctt ggtgaatggt
     1741 ccaagctctt acttcttgta caccagcagt aaagtaagta taaaggttta atattttata
     1801 agtagtttga attaacctat ctaaagcagg ctctttcata ccgtattctt ccataaacat
     1861 ttgtttgtcc tcttcagctt ccatttcggc aatttgtgct tcaatattat tgcacattac
     1921 aattacatct gctccttcgg cctttgcatt tatttttaat gcttcagaat acttattgcc
     1981 cgtgtgcata cttgcttcat ctacattagc cacatatagc acttgttttt cagttagcaa
     2041 aaataaatcg gatatggcta ctctttcttc tttgcttaaa cctaagcttc ttatactatt
     2101 acctttttct aaatgcagtt tacattgcat cagtacatca tattcagcct tcattttaac
     2161 atctgttctc gaaagttttt caactctttg tattttcttt tctacacttt ctaaatcttt
     2221 taactgtaat tcaatatcaa ttatttcttt atcaccaaca gggttaatag ctccttcctc
     2281 tcttaaaaca ttttcatctt caaaacaacg aataacatga ataatagcat ctacctctct
     2341 aatatttgct aaaaatttat tgcctaatcc ttctccttta cttgcaccac gtactaagcc
     2401 agcaatatct acaatttcta attgagtggg tacaattctg tttggcttta ccaattctgc
     2461 tagtttagat agcctataat ctggcacatc tactaaacca gtattgggtt caattgtaca
     2521 aaatctataa ttacttgcct gtgcttttgc actattactt acagcattaa ataaagtact
     2581 ttttcctaca ttgggtaaac caacaattcc tgcttttaat gacataatag tttagaattt
     2641 tatagcacca ttaaataaaa aaatcattaa tgatgaatca atttttaaaa tggctgcaaa
     2701 tgtagcattg taattaagta aaccaaactt taagtactta taatgtgtta atagggtaaa
     2761 ttaagattta tttttttgtt ctttccattg tataattata ctttttatat tggaaaaact
     2821 attagttaaa cattgattta ttttattttc atcggcccaa attacttctt caatatcctc
     2881 ttctacttgt ggtaaaaatg gttcgtttgt tgttatttgc atggcatacc accaggtttc
     2941 ttttattaca tgttggctaa tccatttctc ataatactca tgataggttt tacccacaaa
     3001 attagttata gctacattct gtaaaccggt ttcttcttgt acttctctaa tagcacattg
     3061 ttctatactt tctccttctt caaattttcc ttttggtaaa tcccatttct tttttctata
     3121 tattaatagt aaatcaccat tattattgga tactaaacca ccggctgcaa ttattttttt
     3181 cttttctttc atgttgttgc aatctacatt aattgaatga aatttattta atagtttgta
     3241 gaataacttt tcaataattc attatctgta tgcttataga tgtaaatatt agctttccta
     3301 gtttatcttt gcagcatgac aaatgaaaaa gctgttgcag aaaaactttt acaagtacaa
     3361 gctatacaat taagccctaa tgcaccattt acctgggcca gtggctggaa aagtcccatt
     3421 tattgcgata atagaaaggt tttatctttt ccgtttatta gagattttgt aaagagtgaa
     3481 atgtgtaata ttatttttga aaaatttgaa gatatagaat tgcttgcagg tgtagcaaca
     3541 gcaggtattg cttggggtgc aatggctgca gatcaattaa aattacctta tatatatgtt
     3601 cgtcccaaac ctaaagagca tggcttaggc aatcaaatag agggatttta tgaaaaaggg
     3661 caaaaggtat tggttattga agatttaata tctaccggta aaagtagtat gcaagttgtt
     3721 aatgttttaa gaaaccaagg gttagaagtg gtaggtatag tttctatttt tacctatcaa
     3781 ttttctgagg ctgttcaatt atttgaagaa aataatttac agcatatatc tttaaccaac
     3841 tatactaatt taattgacct tgctattgag aagcagttag taaatgcaga gttgcaaaat
     3901 gttttattag attggagaga taatcctgct acatggaatg gcatttaacc ttttgaaaac
     3961 taggagtgca ttgttgcagc aacagtggta tcaataactc tacttctatc cgttgttaac
     4021 attctgctag gatacctgtt tatcacaata aaatcgtgtg ttgccattat gatagctgta
     4081 ccataatctt tggtaatttg aaataataat tgcattattt catcacttgt ttctgggtct
     4141 aaatttccag taggttcatc tgctaaaatt aattttggcg agtttaataa agcccttgca
     4201 atatctactc tttgttgttc tcctccactc atttcataag gcattttaaa acccttactt
     4261 cttaaaccta ctttatttaa aacatcattt attttttctt ctattaattt ctcatctttc
     4321 caaccagtag cttttaatac aaattttaaa ttttcatgta catttctatc tgtcattaat
     4381 tgaaagtctt gaaaaacaac tcctaagttt cttcttaaaa atggtacttt tttccagttc
     4441 atatctttta aatcaaagcc taccacatta caacttcctt cttttaaggg cagctcgcca
     4501 tataatgttt ttaataagct actttttcct gtacctgttt tgccaacaag ataaacaaat
     4561 tcgccacttt ctatagtaaa gtttacatct tgtaaaataa ggctgttgcc ctgataaata
     4621 tttactcctc ttaattgaac tactgtttct gccatgatat taattttatt tagcaaagct
     4681 aattatacaa aattgtttta aaatgtcttt tgttttcaca aaatgcaata atatgttaag
     4741 aaagttatta aataatattt acttctctct gtaataaaat actaaattta ttgtaaacat
     4801 cattaattat atcttggctc aaattgtata tttcaagccc tgttgcattt ccataattta
     4861 ctaataccaa tgcctgattg gtatggcaac caacatcatt ttttctaaag cctttccaac
     4921 cacaaaattc tattaaccat gcagcaggaa tttttattgt atcatcttta tcgttataac
     4981 ctggtaattt tgggaattgc tgtaatagtt tgtggtattg tgttttggct attactgggt
     5041 ttttaaaaaa actacctgca ttgccaatta ttgctgggtt tggtagtttt gtactacgaa
     5101 tagaaataac tgcatcggat attgctttag cacttaatgc tgttactttc attttatcta
     5161 actcttgttg tatagcacca tagctgtggt taaatatatt ttttttatga agtttaaaag
     5221 taacattaag aatgatgaat tggtttttga atttaccttt aaaaatactt tctctataac
     5281 caaatgcaca atcactgtta ttaaatttta caattgtttt ttcttgtata tgtaaagctt
     5341 ctaactcata aaaagtatct tttatttcta caccgtaggc tccaatattt tgcattgggc
     5401 ttgcccctac attacctggt attaagctta agttttctat accattataa ttattatgaa
     5461 tacagtataa aacaaattga tgccatactt ctcctgcagc tactttaata taatagtgtt
     5521 catcgtcttc acatatcaac tctattccct ttaattcatt ttttaaaaca agcccatcaa
     5581 aatttttagt aaataaaata ttactaccac caccaataat taaagtagta tctgtattgt
     5641 tttctagtag ttcaattaat tgttgaacag agttgaaggc agcaaataat ttagcaataa
     5701 catttattcc aaaagtgtta taaggtttta aagataaatt gtactgtatt tgcatattgc
     5761 aaaataagtt gtttgttcta catttattaa agttgtggca actactttac atattttttt
     5821 aaatgttttt gaagcttgtt ttttggtttg ataatttaat tagatagatt gaataattct
     5881 ttattaatat ttataagata gggtttgtag aaatatggat aatattttta aataaatgcc
     5941 ctagctgtat taattatatt taggtaaggt aaatttttta tcgttgaaga attcaacact
     6001 ttacagtata gtttaataga ataactagaa ctataaactc atcatttctt taaacttcac
     6061 agtttgtctg cggcttactt ctattttctc tcctcctttt agttcaacca acaagccacc
     6121 attaaaataa ggttcaattt tttctatcca acgtaagtta ataatatgtt ttctgtttgc
     6181 tctaaaaaaa gttctttcat caagtctttc ttctaaagcg tttaaagatt ttaatattaa
     6241 tggcttgtta gtactaaaaa atacttttgc ataattgcca acactctcaa ataatcttat
     6301 ttcatgcaat tttacaaacc aacacctttc accatctttt acaaaaacct ggtcttcttc
     6361 tgttaaaggt ccacggccat ttaataaacc agatttttct ttttgaattt cgtattgtaa
     6421 tttttgaatg gcatcgctta atctttttgg ctcaataggc tttaataaat agtctaaagc
     6481 atttacctca aaagctttaa ttgcatattc atcaaaggca gtagtaaaaa ttactctggg
     6541 ggctttttca acttctgcta ataaatcaaa tccagatttg cctggcattt gaatatctaa
     6601 aaaaattaaa tcggggctaa gagtttcaat tttcattatt ccatcatcta catttgctgc
     6661 ttcgtctata atttcaatat ctttatgttc ttgtaataat ttttttaact ctgttcttgc
     6721 aagtctttca tcatctataa ttattgattt tattgccata cttttatttt aataaattaa
     6781 ataatttgat attgttttat gaaattggta gttggattgt agctattact ttattggtta
     6841 ttttatcatc tgttaaatta aaggatgcat ttttgccaaa aagcaatgcc agcctgtttt
     6901 tagtactaga aataccaaat ccatcctgtt ggtaatcatt tccaattaag cctgtattat
     6961 gcactataag ctcatgatgg ttttcaataa atcgagaaat aattttaatt aacccacctt
     7021 cttttgattt acctattcca tgtttaattg cattttcaat taaggtttga agcatcattg
     7081 gtggaatttg ttgatctaaa gtatcttcat ctatttcata ttcaacttgt agtctgtctt
     7141 caaatcttat atgttctaat gctaaataat cttttacaat atccagttct ttttctaaag
     7201 atgtagtttc tgctttatct gtttgcatac tacttctaag caaattgctt aattgggtaa
     7261 ctgcttctct tgcacgtata ggattttcat caaccaatgc cctaatgcta tttaaagcat
     7321 taaaaataaa atgcggatta atatggcttt taatagtttt taactctagt tcttttacta
     7381 cactttctaa ttttatttta tctatttctg ttcttcttgc atcttccaca tagtgccata
     7441 cataataaat acttgtccat actaataaaa taggagacga gtcgaacaaa gtaaaaataa
     7501 atcgtttacc aatggttcca ttaaactctt ttctgtacca accaaggtat tgaattaagg
     7561 cactgctaac aaaactattg gctatacaaa taaatattac tagtataaaa aagtttatcc
     7621 atttactgcc atgtatgggt ggcattaatt taaattgatg aatacaaaag cgtaatacat
     7681 gtgtaaatgc aacacccata aaaacaaata ataatattct tttccaaaaa ataccatcca
     7741 tattgtttct aaataggtaa gagaagaata ccattgaaag ggcataaagt agccatcctc
     7801 ctaattgaaa cagccaataa tttacaagct tctttttcat gacctacaaa tgtaaaaaaa
     7861 tgtaactact aaaaataatg tgttaatgat aagtggtttt gatagtttgt tgatggtaga
     7921 aatatggttt gattggattt attctttcag tttaaatcaa ttttttttta ttaatagatt
     7981 aatttaaaat taaattcata atttttttaa agtattttta ttataattgt aaaagtattt
     8041 ctatactaag ttttttataa ggtaaattgt taaccatggt tgtgaagatt aaagaaaaat
     8101 cattgtgggc taaaatggct gcatataaca tgaaagcttc tcaaatggct atagtttttg
     8161 gtaatactat tcatttacat aatgtaacca gagaagagtt tttaagtaac agaagatggt
     8221 taaggcacga agtggctcat gtaaggcaat atcagcaaaa aggatttata aggtttattt
     8281 tatcctatct ttttgaaact tttaatatga gttacgagtt taataaatat gaagtagaag
     8341 ccaggaaaaa agaaactgat ttaaaagtgt tagaaggggt ggagtttatt taaaaatttc
     8401 gtacacaacc caactcatag agtaagctaa aagcgtcata tatacaaact gaaaaattgg
     8461 ccacttccag gttctagttt ctcttttaac aatagctaaa gtactcatac attgcatagc
     8521 aagcacataa aacatcatta aagataatgc tgttgcaagg gtataaactt tagtgccatt
     8581 tctatgggtg gcagcttgca ttttttctct taaggatgag tcgtcgcttt cttccacact
     8641 atataaagtt gccattgtac ctacaaatac ttctcttgct gcaaatgaag taattaaggc
     8701 tataccaatt ttccaatcat aaccaattgg ttttataatt ggttctattt ttttacctaa
     8761 taaccctgca taagagtgtt ctaatttttc ggtggaggca attttttgca aagaatcgga
     8821 ttgtgttggg ttttgtaaaa ttaattgttc ataatgcaaa tcaatttttt tcatttcatc
     8881 tccagggcca tagctgctta aaaaccataa tagtaagcta attaccatta ttaccttacc
     8941 tgcttctacc acaaatattt tagatttttc aatcattgtt aatccaacat ttttccatct
     9001 tggattacga taaattggta gttctaaaat aaaaaagctt ttctcgttta ttttaatgaa
     9061 ccacttcata acaaagctta ccaacaatgc cataaaagtg ccaaataaat acaatgccat
     9121 cattactaaa ccttgtaaac ttaaaaaacc aaagtagtat ttttccggaa ttgctaaagc
     9181 aattaatata gtataaacag gtaatcttgc actacaactc attaaaggtg taactaaaat
     9241 agtcagtagc ctttcttttc tattttcaat atttcttgta ctcataatgg ctggcactgc
     9301 acaagccaaa ccactaatca ttggcattac actttttcca tttaatccaa ctttacgcat
     9361 tagtttatcg cttaaaaaac taattcttgc catgtagccg gtatcttcta aaatagttat
     9421 taaaccaaac aaaatcataa tttgtggaac aaaaattact attccaccta gccctgcaat
     9481 aattccatta atgaataaat cggtgtacca agttgacggt aaaatatgat taaagaaact
     9541 tgttatactt gcaaaagaag attcaattaa attcattggg tattgagcta accaaaatat
     9601 actttgaaaa agaataaaca gcactgccaa taaaattaaa tatccccatt tacgatgaag
     9661 taaaacatta tctaacttat ctgttattaa ttttttttct gcggggtcgg gggcaaaaac
     9721 atttttttgc attaaacctc ttatacgttg ataacgttgt aaaacctcct cagcctgcac
     9781 tttagttgca ttaaagttgg tagattgtat gatagttgca gtttgctgtt taaatgaatt
     9841 atctacattt aactgttggt aatttattaa ataatgaatt gcggtataag tattaatatt
     9901 gggttgcagg gcttgtatag cttcaatact ttgtggtgct aaggttttat tatctataaa
     9961 atctctgttg tttacaggtg cttgtttagt taatagtagc aaaatttttt tcagttcatt
    10021 agtacccttg ctctttcttg gattaacttc taccacaggt acacccaaat cggtttgtaa
    10081 gccttcaata tcaattttaa ttcctttttt agcagccaaa tcgttcatgg ttaatgctat
    10141 tacaacaggt atttttgtat ctagtatttg gctacaaaac aataaatttc gcttaaggtt
    10201 actagcatct actaccaata aaacaacatc tggtttaatg ttttcatcgg cattcattaa
    10261 aactttataa gccacccact cgtctgctct tcttggatat aatgagtagg tgcctggcaa
    10321 atctataagg ttggcatgta tatcatttct taattctatt ttacccgatt ttttatcaac
    10381 agtaacacca ggaaagttgc ctgttttttg atttaaccct gttaatgtat taaataaggt
    10441 actttttccg ctgttaggat taccaacaat aactatatta aaattttgtt gcaatgagta
    10501 tatttataag atggcgaatg taatagcagc atttttttgt tattatcaaa atcaggaatt
    10561 attatgttaa ggctgctatt ctatacagca aattaagtac aataataaaa aagaagtaga
    10621 agtgtagaaa ttttctttaa tgatattaat actaataact acggagttat caacaattat
    10681 ttataaatta gttgttgaat ttttaaagaa aaatttggtt acaacattca gaacactatt
    10741 tttgcactcc caattaagaa aggtagattc cgtagctcag ttggtagagc aatacacttt
    10801 taatgtatgg gccctgggtt cgagtcccag cgggatcaca aaaaatagct tacagattct
    10861 ctgtaggctt tttttatgct acaatttgtt taaataaata gatatttaca ttttacttca
    10921 accatattta atttctatgt ttttagaaaa attattaaaa tcttttgtaa ataaattagg
    10981 atatgaatta attaaagata aaagaaaatt tataacacag ggtttaacat taccaatgca
    11041 tgtttttact aattgtaaac tattgcctaa tagaaatgca gtattaccat ttcttcctaa
    11101 acaaatggtt atagcagaag tgggagtggc gtatggcgag tttagcaggg aaataataga
    11161 tgttacaaat cctagtaaat tttacgcaat agattattac caaggcgaag aattttgggg
    11221 aagaaataca tttgaaaaaa ataaactatc gcatgaagat tatataaaac aacaattcaa
    11281 taaagagatt gaacaaggta ttttctatac taaaaaagga ttatcgtggg atgtgttaaa
    11341 taccttttcg gataattttt ttgattatgt ttatttagat gcagggcatg attatgaaag
    11401 tgtaaaaaaa gatatagaag tgcttttaaa aaaggtaaaa aatggaggtg tcattcagtt
    11461 taacgactat attatttatg attgggtaag taaattgcct tatggggttg taaaagctgt
    11521 agatgagtta ttacaaaata atcaacacga ggttttatat tattgcctac acaaaggtgg
    11581 gtttaatgat attgtggtaa aaataaataa gtaataagtt agagaaggtg agtttaattt
    11641 aagaacttcc aatgtgtatt tcacattctt tttggttttt taatccttaa ttagtaaatc
    11701 agttaactta aatttgcaaa aaattagaac atgaagaaaa tacatgttgt tatgttggtt
    11761 tgcattgctg cagccattgc tgttttactt ggttttatgg gcaatgttac tacctacgaa
    11821 actattcaat ctgccaaaga aaaacaaggt aaaattgtta ccgttattgc aaaactagat
    11881 actgcaactt tatattacga ccctgtaaaa aatcctaatt atttaacttt tgaagctata
    11941 gactctttag gcgatagaat gaaagtagcc tactattatg aaaaacctta tgatatggaa
    12001 aaaagtgaaa gagtagtttt aaaaggtaaa atggaaaata atgtttttga aataagaaaa
    12061 aaagacggaa tacttgtaaa atgtcctagt aaatacaaag atgatatgga agctgctcaa
    12121 aaaaatttag acgttacaac taaataattt gagttttttg taagctattt aattaaaact
    12181 aacaaccact ttgaatgaaa tatattggag agcatctttt accaggacaa atagggcatt
    12241 tttttattgt actttcttta gtagcatcct tagtagcaac ttttgcattt tactttgcca
    12301 gtaagcaaaa tattttagag gtaaaaaaga gctggttaaa gtttgcacgt attgcttttt
    12361 tattagagtc aatttcggta ttggctgttt ttgttttatt atattatatc atctctcatc
    12421 atttatttga atataaatac gcttggcaac acagcgataa acaattacag ttagagtatt
    12481 tattaagttg tttttgggaa ggacaagaag gaagtttttt actttggagt ttttggcatt
    12541 gtgtattagg atggatttta atttggaaag cgaaagattg ggaagctggt gtaatgactg
    12601 tggtaagttt tgcccaattt tgtttagcta caatgacttt aggtatatat ttctttggcc
    12661 ataaagtagg tagcaatccg
//
LOCUS       DAOQPK010000043        12665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_2554_length_12665_cov_4.513243, whole genome shotgun sequence.
ACCESSION   DAOQPK010000043 DAOQPK010000000
VERSION     DAOQPK010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12665
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2554_length_12665_cov_4.513243"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PLU36_03500"
     CDS             <1..280
                     /locus_tag="PLU36_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HMZ45846.1"
                     /translation="IPGVGKAIKDVEVDNSSFKGIEAIIQSMTLEERANPDLINPTRK
                     SRIAKGCGKDIAEINAFIKQFEQMKKMMSMMNKMPMGGLSNMMKGMKR"
     gene            complement(404..1099)
                     /locus_tag="PLU36_03505"
     CDS             complement(404..1099)
                     /locus_tag="PLU36_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ45847.1"
                     /translation="MEGRKYRILLCEDDTNLGMVLKNYLELNDYDVVLERDGRLGLAA
                     FQREKFDICLLDVMMPNMDGFTAAEEIRDIDPDIPLFFLSAKTMKEDIIQGYKLGADD
                     YITKPFDSEVLLHKIKAILKRNEEETKVNENIEFDLGTYHFNPKLRELIREGSTQTLS
                     PKENDLLKMLAEHKNDLLPRERALKKIWGSDTYFNGRSMDVYIAKLRKYLKEDEKIEI
                     VNIHGNGFRLVAP"
     gene            1324..2538
                     /locus_tag="PLU36_03510"
     CDS             1324..2538
                     /locus_tag="PLU36_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HMZ45848.1"
                     /translation="MNIKRKIINDPVYGFITIDHPILFKIIAHPYYQRLRRIHQMALA
                     YLVYPGAVHTRLHHSLGAYHLMCLAITELKAKGTEITEEEEIAVKAAILLHDIGHGAF
                     SHALEKQILNNIHHEELSLKMMKVINSELNGQLDTTIAIFTNNYPKKFLHQLVSGQLD
                     VDRMDYLTRDSFFTGVSEGVIGYDRIIKMLIVHNGELMVEEKGIYSIEKFLVARRQMY
                     WQVYLHKTVVAAEVMLVNILKRVNELSVKDKTGLQTDGALDYFLFEYTNGLNLQVVEK
                     FSELDDSDLSFAIKKWSKHKDFVLSTLCKKLLARNLYKIKLQSKPFNSEEINELQVKA
                     MQQFKVDSSLIPYFVHTGTANNTMYKTEDERINILYKDGTVKDISAIDNPLIHQTISA
                     PIKKFYICLLEP"
     gene            2571..3620
                     /gene="lpxD"
                     /locus_tag="PLU36_03515"
     CDS             2571..3620
                     /gene="lpxD"
                     /locus_tag="PLU36_03515"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HMZ45849.1"
                     /translation="MFTAQQIALLVNAKIEGDPNIIVSSFGKIEEAKEGQLTFFANPK
                     YEDFLYTTTASVVLLNENFVLKLPIKATLLRVADAYSAFAILLNKYQAIQAQQLEGIQ
                     QPVYISPSAKIDEKVFIAAFTYIGENVKIGKNVKLFAGVSLGNNVEIDDNTILHAGVK
                     IYEGCKIGKNVIIHAGTVIGGDGFGFAPQANGSYQKIPQIGNVIIEDFVEIGANTTID
                     RATIGSTIIRTGVKLDNLIQIAHNVEVGNNTVIAAQAGVSGSTKIGKNVMIGGQAGIV
                     GHITVADGSKINGQSGVSKSITEPNQAVTGSPAYDYTAALRSQAVVRKLPELEKRIKE
                     LENQLSQILALESKS"
     gene            3652..4554
                     /locus_tag="PLU36_03520"
     CDS             3652..4554
                     /locus_tag="PLU36_03520"
                     /inference="COORDINATES: protein motif:HMM:NF016142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HMZ45850.1"
                     /translation="MNTFLKLSDEVANALQQKKPVVALESTIIAHGMPYPKNVETALA
                     VEQTIKNNGATPATIAIINGKCCVGLTAEEIEIFGKEKEVWKVSLRDMPYVISKKLFG
                     ATTVAATMRIAAMAGIKIFVTGGIGGVHRGAEKTMDISADLSEMAETNVAVVCAGVKS
                     ILDIGLTLEYLETKGIPVVTYGQKSFPSFYSTESNYNTPLQLNSSKEIAEMLQTKWAL
                     GLNGSVLIANPINKKDEVPFAVIESHITNALEHAEKQGIKGKETTPFLLQHIAQHTNG
                     NSLEANISLIKNNAVLGAQIAAHL"
     gene            4566..5081
                     /locus_tag="PLU36_03525"
     CDS             4566..5081
                     /locus_tag="PLU36_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45851.1"
                     /translation="MNIFSPYKINSFVMGSNINIELANWRKNINYITLRIFPFVLSVI
                     TVYAVILNFKEMPKAYSYVLLIGIPALSVYMFFTKYSVQTIIKPPYIQVYKNSFRGKE
                     ESNINFNSIHKLTCKIRYGKYGGTFFYLHTSANKKIEFITIPVTNMKKEYTDLIIKEL
                     EAIVGKTVEFV"
     gene            5148..6158
                     /locus_tag="PLU36_03530"
     CDS             5148..6158
                     /locus_tag="PLU36_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HMZ45852.1"
                     /translation="MKFGVIGSGSWATALAKILTDNGNHINWWIRNTDTISYLHTRKH
                     NPRYVSSAYFNTQQLYMSNEITEVVEKSNVLVIATPSAFTEDVLKQLPSNAFEGKIII
                     SAIKGILPSENDLLNEYLKKHFNVPIENYCTILGPCHAEEVASEKLSYLTISNINESL
                     ALSIAKHFNTEYLNTRVNHDISGVQYAAILKNIYALGAGIAHGLEYGDNFLSVYIANA
                     ADEMVAFLKQLVWNKETEEEQRKINYAASVYLGDLLVTCYSLYSRNRTFGNMIGKGYS
                     VKAAQLELNMIAEGYNASKCIFKTNQTVNANIPIATIMYKILWEQLPAEIGFKEIEKL
                     LI"
     gene            complement(6244..6744)
                     /locus_tag="PLU36_03535"
     CDS             complement(6244..6744)
                     /locus_tag="PLU36_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HMZ45853.1"
                     /translation="MLSKTMQEALNRQVLMEAQSSQAYLAMASWAEIQPGLKGVTEFF
                     FKHSDEERMHMLKLVHYINERGGFAVIPSLEQPVLTFVSLKKAFEELLKHELAVSNSI
                     NELVDISLKEKDYATHNFLQWYVTEQMEEERLARECNDKLEMIGDDKSGLYLFDRDIM
                     SMENGG"
     gene            6918..8990
                     /locus_tag="PLU36_03540"
     CDS             6918..8990
                     /locus_tag="PLU36_03540"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ45854.1"
                     /translation="MLKFKPLFLLLLLFNFANFKIFAQQDTIKGVGSEVVVTGQYSPQ
                     PLRSSVYKVKTITAERIKMRAATDIVGVLNSELGIRFSTDNSLGETDTKILGLGGTRV
                     KILIDGVPMADRDATKQSINQIDINSIEKIEIVEGPMSVIYGTDALAGVINIITKKGS
                     KNGNHLSVGLKIQEEGIGNTYVPFTKNGIHNQNLSVNWNNNKYRASAYLTRNDFGGFA
                     DTAAYPAKVFRPKKQWMTGGTVGYKNSIFDIWYRVDFLHEELLSASPMSLTTYKSFRQ
                     YYFTKRYTHQLQSIWNINKELKLNSAVSFQDYNRTTESYTLDYRTGINSPNKTKQDIE
                     SGYWDVTPFQSLFVRTALSWIVSSKVSLQPGLEFKQDKTSGERVSGSPSISDFSFFIS
                     SEIKPTSKINIRPGLRLSKNSVYDAPPIIPSINTKFTLNKLLDLRLSYARGFRAPILR
                     ELYFNFYDANHNIKGNTNLKAETSNSFMTTITCNAITKNHFSYASTITGFYNSYKDFI
                     DLYDYIDNNGVATFSYFNRDNFKTKGITFENNLSYKNLVAGIGLSYIGYYNRFQENNT
                     LQGNTAKFAWSPEISTNITYHLKKLKGSIGMYYKFTGQIPTFSLGTNNDIVLSKRNAF
                     HWADLTATKQLFKLITLQAGIKNLFDVGRLGSTSTIAGNQHGAGSVINYAYGRSYFIT
                     INFQWSKK"
     gene            9019..10065
                     /locus_tag="PLU36_03545"
     CDS             9019..10065
                     /locus_tag="PLU36_03545"
                     /inference="COORDINATES: protein motif:HMM:NF025430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HmuY family protein"
                     /protein_id="HMZ45855.1"
                     /translation="MKNHFKILTPSLLILSILFTACERDQMPPLIPPSTGGDTTLNIL
                     AGSESGAYAANSAFIDFSTDIISTSGRASWDVAFYCGSDFKVGLNYKTVALTKQINKN
                     DLTNVTAADTIGMALSLSYSSTDFELLDSFSGNLEYTKIPVNTTAADNKVFIVQRGTG
                     AGIAAKPWYKIRVLKNSNISYTLQYARITETTFKTIEITKDNNYNFKFVSFDNGLVLS
                     EPKKDDWDIQYTAAVYKFPMGPVEIPYIFSDMVLINYLGGVQAAQVLNTQFSYNTITL
                     ANAQSLTYSNDRWAIADKWRTSTSSPTAGVKTDRFYVIKDQIDNYYKLKFISFHNTEG
                     GTRGKPKIAYELIK"
     gene            complement(10158..11360)
                     /locus_tag="PLU36_03550"
     CDS             complement(10158..11360)
                     /locus_tag="PLU36_03550"
                     /inference="COORDINATES: protein motif:HMM:NF013559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallopeptidase"
                     /protein_id="HMZ45856.1"
                     /translation="MKTIKQLLLGMAVICTTVVTAQDKKVYKKVGCATVEPIQQVFAF
                     DTTTAGKRGLADNYYLWDNGKTLYVKIMNGSKKYKDMIKKYAKDWEKYANIKFEFVEV
                     GNTHIRVVLTGNDGGGNYSKLGTMALMVPQDEHTMHLDTTSFKTPEYTYTCIVHEFGH
                     AIGLMHEHMNPTSGIKWDKNRIYASYKLNQGWDKAMVDAQLFKKYSINYTNGTKYDPK
                     SIMHYPISRWETTDGFHVEWNNSISEGDKQLISALYPKKGARVNEVPRFTVSDFTQMQ
                     VERSDSKEGVSFFPSFTINTAGKSGRVYYLAVFTDDQGNMFETSSNEYKVFGVSGTSK
                     TSVLSPGQKIVVNKGKKDLELFIPYSEIPEKIRNKNIRASFYVFLYDNDEMKLLYASK
                     SVPCNMAR"
     gene            complement(11410..12615)
                     /locus_tag="PLU36_03555"
     CDS             complement(11410..12615)
                     /locus_tag="PLU36_03555"
                     /inference="COORDINATES: protein motif:HMM:NF013559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallopeptidase"
                     /protein_id="HMZ45857.1"
                     /translation="MKKIKLIVLIALSSVFCVNLNAQKKIKQPVVQKNGCSSVQPIAN
                     VFLADTIPGRGLADNYYLWDNGKTIYVKFLSGSKLMQDKVKTIAKEWEKYANIKFKFV
                     EVGNSHIRINLDDKGGHNSTIGTLALSVPNDERTMNFDTTDFKTYAAMYRTVLHEFGH
                     ALGLLHEHYSPVSGIKWNKEAVYNELKKTQGWSKEMVDANLFQQFNQNYTNGTSYDSK
                     SIMHYPIKANWTTNAYSVNWNNKLSDGDIAMIKTLYPVGERENEVPRFVISNFTSLKV
                     QNNDNKQGVSIYPSFTLSTAGKSATVYFVAFFYDEDGNAIQKTTKSYSISGVTATFKS
                     FIIAADKTLNPNKNKASELELFIPYNELPVIKGKNNFQVEFKVYMLDGRELKLLYQSK
                     RKVFNFMKS"
BASE COUNT         4549 a         1900 c         1879 g         4337 t
ORIGIN      
        1 gatacctggt gttggtaaag caataaaaga tgttgaggtt gacaacagca gttttaaagg
       61 tattgaagcc attattcaaa gtatgacact tgaagaaagg gctaaccctg atttaattaa
      121 cccaacccga aaaagcagaa ttgcaaaagg ttgcggaaaa gatattgccg aaattaatgc
      181 ttttataaag cagtttgaac aaatgaaaaa aatgatgagt atgatgaata aaatgccaat
      241 gggtggttta tcaaatatga tgaaaggaat gaaacgctag agaataaaat ttttcaatat
      301 attttagcca agtttatgta aactattaat taactaaaat tattttcaaa ataaaaagcc
      361 ttgtaaaaaa aattacaagg cttttttagt gtacaaatta tagttatgga gccactaatc
      421 taaatccatt gccatgaata ttaactattt caattttttc atcttctttt aaatacttac
      481 gaagtttagc aatatataca tccatacttc taccattaaa ataagtatca ctaccccaaa
      541 ttttcttcaa tgctctttct cttggtaata aatcattttt atgctcggca agcattttta
      601 ataaatcatt ttcttttggc gataaagttt gagtgctccc ttctctaatc aattctctta
      661 atttaggatt aaaatgatag gtacctaaat caaactctat attttcgttt acctttgttt
      721 cctcttcgtt tctttttaaa attgctttta ttttatgtaa taaaacttca ctgtcaaatg
      781 gtttggtaat ataatcatcg gctcctaatt tatagccttg tattatatct tctttcatag
      841 ttttggcact taaaaaaaat aaaggaatat ccggatctat atctctaatt tcttcagcag
      901 cagtaaatcc atccatattg ggcatcataa catccaataa acatatatca aatttttctc
      961 gctgaaatgc agctaaacca agccttccat ctctttctag tactacatca taatcgttca
     1021 actctaaata attttttaaa accatgccaa gatttgtgtc atcttcgcaa agcagaattc
     1081 tgtattttct tccttccata atgttaatga tttgtattaa agatataaat gaattgtaaa
     1141 agtcatacca cttattcaaa aggttttgtt aaagaagtaa aaaacaggca aatatttgta
     1201 ttttgggcat ctcaaagtaa ataaatactt atagtgggca ttgaaattat tactacccta
     1261 atataaacta taaactaata aataaaattt gatagtatct tcattttatt tttttaacaa
     1321 cttatgaata taaaaagaaa aattattaac gaccctgttt atggttttat cacaatagat
     1381 catcctatat tatttaaaat aattgctcac ccatattatc aaaggctaag aagaatacat
     1441 caaatggcat tggcttattt agtatatcct ggtgcagttc atacaaggct tcatcacagt
     1501 ttaggggcat accatttaat gtgtttagca ataacagaat taaaggcaaa gggaacagaa
     1561 attacagaag aggaagaaat tgcagtaaag gcagctatat tattgcatga tattggccat
     1621 ggagcttttt ctcatgcatt agaaaaacaa attttaaata acatacatca cgaagagttg
     1681 agccttaaaa tgatgaaagt tattaatagt gaattaaacg ggcaattaga tactacaata
     1741 gccattttta ctaataacta tcctaaaaaa tttttacacc aattagttag cgggcaatta
     1801 gatgtagata gaatggatta tttaaccaga gacagttttt ttacaggtgt aagtgaaggg
     1861 gtaattggtt acgatagaat tattaaaatg cttattgtac ataatggtga gctaatggta
     1921 gaagaaaagg gtatttatag tattgaaaaa tttttagtag ccagaaggca aatgtattgg
     1981 caagtttatt tacataaaac tgtagtggct gccgaagtta tgttggtaaa tattttaaaa
     2041 agagttaacg aattaagcgt aaaagataaa acagggctac aaacagatgg tgctttagat
     2101 tattttttat ttgaatacac taatggttta aatttacaag tagtagaaaa atttagcgaa
     2161 ttagacgata gtgatttaag ttttgccata aaaaaatgga gtaaacacaa ggattttgtt
     2221 ttgagcaccc tttgtaaaaa actattagcc agaaacttat ataaaataaa acttcaaagt
     2281 aaacctttta attctgaaga aataaacgaa ttacaagtaa aagcaatgca gcaatttaag
     2341 gtagatagta gtctaattcc ttattttgta catactggta ctgccaataa tacaatgtat
     2401 aaaacagaag atgaaagaat aaatatatta tataaagatg gaacagttaa agacatttca
     2461 gcaatagata acccattaat acatcaaact atatcggcac caataaaaaa attttacatt
     2521 tgtttacttg aaccctaaaa agtatattta ctatacagca taattataaa atgtttacag
     2581 cacaacaaat agcattatta gttaatgcaa aaatagaagg agacccaaat attattgttt
     2641 catcttttgg aaaaatagaa gaagctaaag aagggcagtt gacatttttt gccaacccta
     2701 aatacgaaga ttttttatat actaccaccg cctcagttgt tttactaaac gaaaactttg
     2761 ttctaaaact acctattaaa gcaaccttgc ttagagtagc agatgcttac tctgcttttg
     2821 caattttatt aaacaaatac caagcaatac aagcacaaca attagaaggc attcaacaac
     2881 ctgtttatat ttcgccatcg gcaaaaattg atgaaaaagt ttttatagca gcttttactt
     2941 atataggaga aaatgtgaag attggaaaaa atgtaaaact atttgctggc gtatctcttg
     3001 gtaataatgt tgaaatagac gataacacca ttttacatgc aggagtaaaa atatacgaag
     3061 gttgtaaaat tggaaaaaat gtaattattc atgcaggaac agtaattggt ggagatggtt
     3121 ttggttttgc cccacaagcc aatggtagtt atcaaaaaat acctcaaata ggaaacgtaa
     3181 ttattgaaga ttttgttgaa attggagcaa acacaacaat tgaccgtgca acaattggta
     3241 gcacaataat aagaacaggt gttaagttag ataacttaat acaaattgcc cacaatgtag
     3301 aggttggtaa caatacagta attgcagcac aagcaggggt tagtggaagt acaaaaattg
     3361 gtaaaaatgt aatgattggt ggacaagcag gaattgttgg ccatattact gtagcagatg
     3421 gtagcaaaat aaatggacaa agtggtgtaa gcaaaagtat tactgaaccc aaccaagctg
     3481 ttacaggctc accagcttat gattatactg ctgcattaag aagccaggca gttgtaagaa
     3541 aattacctga actagaaaaa agaataaaag aattagaaaa tcaattatcg caaattttag
     3601 ctttagaatc aaagtcttaa catatttcta ttattctatt acttctattt aatgaataca
     3661 tttctaaaat tatctgatga agttgcgaat gctttacaac aaaaaaaacc tgttgttgct
     3721 ttagaaagta ccattattgc tcatggtatg ccctacccaa aaaatgtaga aacagcatta
     3781 gctgttgaac aaacaataaa aaataatggt gccacacccg ctacaattgc aattattaat
     3841 ggaaaatgct gtgtaggctt aaccgctgaa gaaatagaaa tttttgggaa agaaaaagaa
     3901 gtatggaaag tgagtttaag agatatgcct tatgtaattt ctaaaaaatt atttggggca
     3961 actactgtag ctgctacaat gcgtattgca gcaatggcag gtattaaaat ttttgtaaca
     4021 ggaggtattg gaggtgtgca tagaggtgct gaaaaaacta tggatatcag tgcagatctt
     4081 tcagaaatgg cagaaacaaa tgttgctgtt gtatgtgctg gtgtaaaatc tattttagat
     4141 attggcttaa ctttagaata cttagaaacc aaaggaattc ctgtagtaac ttatggccaa
     4201 aaatcttttc caagttttta ttcaaccgaa agtaattata acactccatt gcaattaaac
     4261 tcatctaaag aaattgcaga aatgctacaa accaaatggg cattgggttt aaatggttca
     4321 gtacttattg ccaacccaat taataaaaaa gatgaagtgc cttttgcagt tatagaaagc
     4381 cacattacta atgctttgga acatgctgaa aaacaaggaa taaaaggaaa agaaactacc
     4441 ccttttttat tacaacatat tgctcaacat actaatggca atagtttaga ggcaaatatt
     4501 tctttaataa aaaataatgc tgtacttggt gctcaaattg ctgctcattt ataattattt
     4561 gtataatgaa cattttttct ccgtataaaa taaattcatt tgtaatgggt agtaatatta
     4621 atattgaact agctaattgg agaaaaaata ttaattatat aacacttaga atttttcctt
     4681 ttgtattatc tgttataact gtttatgctg ttatattaaa ttttaaggaa atgccaaaag
     4741 catattctta tgtattatta attggtattc cagcactttc tgtttatatg ttctttacta
     4801 aatattctgt tcaaaccatc attaaacctc catacataca agtgtataaa aattctttca
     4861 gaggcaaaga agaatcaaat attaatttta atagcattca taaattaacc tgtaaaataa
     4921 ggtatggcaa atacggagga actttttttt acttgcatac ctcagccaat aaaaagatag
     4981 agtttataac tattccggtt acaaacatga agaaagaata tacagattta attattaaag
     5041 aactagaggc tattgtaggc aaaacagttg aatttgttta agctacatta taaaaaactt
     5101 acaaacttca aaacattatc tttgttaggc actaatacaa ttgtactatg aaatttggag
     5161 ttattggtag cggaagttgg gctactgcac ttgcaaaaat tttaaccgat aatggtaatc
     5221 acattaattg gtggattaga aacacagata ccatttccta tttacataca agaaaacata
     5281 atccacgtta tgttagtagt gcttatttta atacccagca actttatatg agtaatgaaa
     5341 ttactgaagt tgttgaaaaa agtaatgttt tggttattgc taccccttct gcttttacag
     5401 aagatgtgtt gaaacaatta ccaagtaatg cttttgaggg gaaaataatt atttctgcca
     5461 taaaaggtat tctaccttca gaaaatgatt tattaaatga atatttaaaa aaacatttta
     5521 atgtacctat agaaaattat tgtaccatac ttggcccctg ccatgcagaa gaagtagctt
     5581 cggagaaact aagctattta acaatttcaa atattaatga aagccttgca cttagtatag
     5641 caaaacattt taatactgaa tacttaaaca caagagtaaa tcacgatatt tcaggcgttc
     5701 aatatgctgc aatattaaaa aacatttatg ctttaggtgc aggtattgca catggtttag
     5761 aatatggaga taatttttta agtgtttata ttgcaaatgc tgcagatgaa atggttgctt
     5821 ttttaaaaca actggtttgg aataaagaaa ctgaagaaga acaaagaaaa attaattatg
     5881 cagcatctgt ttatttggga gatttattgg taacctgtta ttctttatat agcagaaaca
     5941 gaacttttgg aaacatgatt ggaaaaggat atagtgtaaa agcagcacaa ttagagctta
     6001 atatgattgc tgagggatat aatgcaagta agtgtatttt caaaaccaac caaaccgtta
     6061 acgcaaatat tccaattgct acaataatgt ataaaatttt atgggagcaa ttaccagcag
     6121 aaattggatt taaggaaatt gaaaaattat taatttaaaa aagccgttat actaataacg
     6181 gctagtaaaa aataaactat tctataagtt aagcaagtga atacttacat tcagaaattg
     6241 tttctaacct ccgttttcca tactcattat atctctatca aataaatata aaccactttt
     6301 atcatcacca atcatttcta atttatcgtt acattctctg gcaagtcttt cttcttccat
     6361 ttgttcggtt acataccact gcaaaaaatt atgtgttgca taatcttttt cttttaaaga
     6421 aatatctact aactcattta tgctattgct tacagccaac tcatgcttca ataactcttc
     6481 aaatgctttt ttcaatgaca caaaagttag cacaggttgt tctaaagaag gtattactgc
     6541 aaagccacct ctttcattaa tatagtgtac taattttagc atgtgcattc tttcctcatc
     6601 actatgttta aagaaaaatt cagttacccc ttttaatcca ggctgaattt ctgcccaact
     6661 agccattgct aaataagctt gagaagattg agcttccatt agcacttgcc tatttagtgc
     6721 ttcttgcatt gttttagata acatactttt tgttttactc aaagttaaaa aaatacatta
     6781 ttaatataac aacatactgg tttttatttt taaaaaaaga atattaactg attaacattt
     6841 gacaattcta tggcagattt taaatctata tcattgaaaa tttgcagata tttaaaaaac
     6901 tgctttaaac ttactatatg ttaaaattta aacccctctt tttactactg ctcttattta
     6961 attttgccaa ctttaaaatt tttgcacagc aagatactat taaaggagta ggcagcgaag
     7021 tggtagttac aggtcaatac tctcctcaac cattaagaag ttctgtttat aaagttaaaa
     7081 caattactgc agaaagaatt aaaatgagag ctgctacaga tattgttggt gtattaaaca
     7141 gtgaattagg aataagattt tctaccgata attctttagg agaaacagat acaaaaattt
     7201 taggcttagg tggcacacgt gtaaaaattt taattgatgg tgttccaatg gcagatagag
     7261 atgcaaccaa acaaagtatt aatcaaattg atataaactc aatagaaaaa atagaaattg
     7321 tagaaggacc aatgagtgta atttatggta ccgatgcact tgcaggtgtt ataaacatta
     7381 ttacaaaaaa aggttctaaa aacggaaacc atttgtcggt tggtttgaaa attcaggaag
     7441 aaggcattgg taacacctat gtacctttta ccaaaaatgg tattcacaac caaaatttat
     7501 cagttaactg gaacaataat aaatacagag cttcggctta tttaaccaga aatgattttg
     7561 gtgggtttgc tgatacggct gcttatcctg caaaagtttt tagacctaaa aaacaatgga
     7621 tgacaggtgg tacagtaggt tataaaaata gcatttttga tatttggtat agagtagatt
     7681 ttttacatga agaattgctt tctgcaagcc caatgagcct tacaacttat aaaagtttta
     7741 gacaatacta ctttactaaa agatataccc atcaattaca atctatttgg aatataaaca
     7801 aagaattaaa attaaattct gctgtttctt ttcaagatta taatagaaca acagaatctt
     7861 atacgcttga ttacagaaca ggaattaact ctcctaataa aacaaaacaa gatattgaaa
     7921 gtggctattg ggatgttacc ccctttcaat ctttatttgt ccgtactgca ttatcttgga
     7981 tagtttcttc aaaagtttca ttacaaccag ggttagagtt taaacaagat aaaacaagtg
     8041 gagaaagggt ttctggctct ccttctattt ctgatttttc attttttata tcctcagaaa
     8101 taaaacctac atcaaaaatt aatatacgcc ctgggttacg attaagtaaa aattctgttt
     8161 acgatgcacc acccattatt ccttctatca atacaaaatt tacactaaat aaattgttag
     8221 acttacgctt gtcttatgca agaggtttta gggccccaat tttaagagaa ttatatttta
     8281 atttttacga tgctaaccat aatataaaag gaaataccaa cttaaaagcc gaaacatcta
     8341 acagctttat gacaaccatt acgtgtaatg caattaccaa aaatcatttt tcttacgcat
     8401 caaccataac aggtttttat aacagctata aagattttat tgatttatat gattacatag
     8461 ataacaatgg tgttgcaact ttctcttact ttaatagaga taattttaaa acaaaaggaa
     8521 taacttttga aaataattta agctataaaa atttagttgc aggtattggg ctttcatata
     8581 ttggatacta taataggttt caagaaaaca ataccctaca aggcaataca gctaagtttg
     8641 catggtcgcc tgagatttct actaacatta cttaccattt aaagaaactt aaaggtagta
     8701 ttggcatgta ttataaattt acgggtcaaa ttcctacttt ctcattaggc actaacaatg
     8761 atattgtttt atcaaaaaga aatgcatttc attgggccga tttaacagct acaaagcaat
     8821 tatttaaact tattacctta caagctggca ttaaaaattt atttgatgtt ggaaggttag
     8881 gaagtacatc aaccatagca ggcaatcagc atggtgcagg ttctgttatt aactatgcct
     8941 atggtcgttc ttattttata acaataaatt ttcagtggag taaaaaataa attattaaat
     9001 acatagaaat ataaaaacat gaagaatcat tttaaaatat taaccccatc cttattgatt
     9061 ttatctattt tatttactgc ttgcgaaaga gatcaaatgc ctcctttaat tccaccttct
     9121 acaggaggag atactacatt aaatatttta gcaggaagtg aaagtggagc ttatgcagcc
     9181 aactctgctt tcatagattt tagtacagat attattagta catctggcag agcaagttgg
     9241 gatgtagcct tttactgtgg cagtgatttt aaagttggat taaactataa aacagtagct
     9301 cttacaaaac aaattaataa aaacgattta acaaatgtaa ctgctgccga tactattggt
     9361 atggctttat ccttatctta ttcttcaacc gattttgaac tactagatag tttttcagga
     9421 aatttagaat atacaaaaat tccggtaaat acaactgctg ccgataataa agtatttatt
     9481 gtacaaaggg gaacaggtgc aggtattgca gcaaaaccat ggtataaaat aagagtatta
     9541 aaaaatagca atattagcta caccttacaa tatgcaagaa ttacagaaac aacttttaaa
     9601 acaattgaga taaccaaaga caataattac aattttaaat ttgtttcttt tgataatggt
     9661 ttggttttat ctgaacctaa aaaagatgat tgggatatac aatatactgc tgcagtttac
     9721 aaatttccaa tgggcccggt agaaattcct tatatttttt cagatatggt tttaattaat
     9781 tatttaggtg gtgtacaagc tgctcaagta ttaaacacac aattttctta taacaccatt
     9841 actttggcta atgcacaaag tttaacttat agtaatgata ggtgggcaat agcagataaa
     9901 tggcgtacat caacatcaag ccctacagca ggtgttaaaa cagatagatt ttatgtaata
     9961 aaagatcaaa ttgataatta ttataaacta aaattcatca gctttcataa tacagagggt
    10021 ggcacaagag gtaaaccaaa aattgcttac gaactaatta aataattttc taaaattcat
    10081 tgtaataatt ctaagtggtt aaatataaaa agaggctact tatgggtagc ctctttaatt
    10141 tttattaatt ttacttatta cctagccata ttacatggta cacttttaga ggcatataat
    10201 aatttcattt catcattatc gtataagaaa acataaaacg aagctcttat atttttattt
    10261 cttatttttt ctggaatttc agaataagga ataaataatt ctaaatcttt tttgccttta
    10321 ttcactacaa ttttttgacc aggactaaga acagatgttt tagatgtacc acttacacca
    10381 aatactttgt actcattact agatgtttca aacatattgc cttggtcatc tgtaaacact
    10441 gctaaataat atactctacc actttttcct gctgtattta ttgtaaagga tgggaaaaag
    10501 cttacccctt ctttagaatc acttctttca acctgcattt gagtaaaatc gctaacagta
    10561 aagcgtggca cttcattaac ccttgctcct tttttaggat ataaagccga tattaattgt
    10621 ttatctcctt cagatatgct gttgttccat tctacatgaa atccatcggt agtttcccaa
    10681 cgtgaaatag gatagtgcat gatagattta ggatcgtatt ttgtgccatt agtataatta
    10741 atgctatatt ttttaaataa ttgagcatct accattgctt tatcccaacc ctgatttaat
    10801 ttgtaagatg cgtaaattct atttttatcc catttaatgc cagacgttgg gttcatgtgt
    10861 tcatgcatta aaccaattgc atgcccaaac tcatgtacaa tacaagtata agtatattca
    10921 ggtgttttaa atgatgtagt atctaagtgc atagtatgtt catcttgagg taccattaag
    10981 gccattgttc ctaatttaga ataatttcca cctccatcat ttcctgtaag tacaacacga
    11041 atatgggtgt taccaacttc aacaaattca aattttatat tggcatattt ttcccaatct
    11101 ttagcatact tctttatcat gtctttatac tttttacttc cattcattat tttaacataa
    11161 agtgttttgc cattatccca cagataataa ttatctgcta aacctctttt gcctgctgtt
    11221 gtagtatcaa aagcaaatac ttgttgaatg ggttctactg tagcacaacc aactttttta
    11281 tatacttttt tatcttgtgc tgttacaacg gttgtgcata ttactgccat acctaataat
    11341 agttgtttta tcgttttcat tgttttcatt gtttataatt ttaatgtttt tatatagatg
    11401 tattttgttt taagatttca taaaattgaa tacttttctt ttagactggt ataaaagttt
    11461 taattctcta ccatctaaca tataaacttt aaactctacc tgaaaattat tttttccttt
    11521 tatcacaggt aattcgttat aaggtataaa aagttccaat tcgcttgctt tatttttatt
    11581 tggatttaaa gttttatctg cagcaataat aaatgattta aaagttgcag ttacaccact
    11641 aatactataa gatttagttg ttttttgaat tgcatttcca tcttcatcat aaaaaaaggc
    11701 aacaaaataa actgtagctg acttgcctgc tgtacttagt gtaaaagaag gatatattga
    11761 tacaccttgc ttattatcat tgttttgaac ttttaaagat gtaaagttag atattacaaa
    11821 acgaggtact tcattttctc tttctcctac tggatataaa gttttaatca ttgcaatatc
    11881 tccatcagat aacttattgt tccaatttac agaataagca ttagttgtcc aatttgcttt
    11941 tatagggtag tgcataatag atttagagtc gtaacttgta ccattggtat agttttgatt
    12001 aaattgttga aataaattag catcaaccat ttctttagac cagccttgtg tttttttcag
    12061 ttcattatat actgcttctt tattccattt tattcctgaa actggactat aatgttcgtg
    12121 taacaaacct aatgcatggc caaactcatg taaaactgtt ctatacatgg ctgcataggt
    12181 tttaaaatcg gtagtatcaa aattcattgt tctttcatca tttggtacag agagagccaa
    12241 tgtaccaatt gtggaattat gcccaccttt atcatctaaa tttattctaa tatggctgtt
    12301 tccaacttct acaaacttaa attttatatt agcatatttt tcccattctt tagctattgt
    12361 tttaacttta tcttgcatta atttactgcc gcttaaaaac tttacataaa ttgtttttcc
    12421 gttatcccac aaataataat tatccgctag ccctctacca ggtattgtat ctgctaaaaa
    12481 tacattagca ataggctgta cactgctaca accatttttt tggactactg gttgttttat
    12541 ttttttttgt gcatttaagt ttacacaaaa cacacttgat aaggctatta atactattaa
    12601 ttttattttt ttcatattcg tttaaattct attctttaaa aaattaaacc ctttcaataa
    12661 tagta
//
LOCUS       DAOQPK010000044        12395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_2615_length_12395_cov_5.000324, whole genome shotgun sequence.
ACCESSION   DAOQPK010000044 DAOQPK010000000
VERSION     DAOQPK010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12395
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2615_length_12395_cov_5.000324"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..1389)
                     /locus_tag="PLU36_03560"
     CDS             complement(100..1389)
                     /locus_tag="PLU36_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit I"
                     /protein_id="HMZ45858.1"
                     /translation="MFVGLGNSSIVKTTSYKVVLPFYLYAAISFIVASIFLLLSSNTF
                     LAHYFQPHILAITHIMALGWGTMIILGASHQLVPVLIEGELYSYKLAHLSFILSAIGI
                     PLLVYGFYVFDMGTPAKWGGRLVLLSILCYLINIGYSISKSKKENVHAVYVFTSVIWL
                     FITVSLGLALVYNFTYNFLPQNSLHYLPLHVHTGILGWFLLLVIGVASRLIPMFLISK
                     YTNPTILWIIFWLINTALISFIIIFFYLPLSYTLIPDYLLFVAIVLFIYYCYNAYKQR
                     IRKQVDEQVKLSLLSTVLMLLPILLLFIIIILFINSSAENYQPMVAYGFMIFFGWITA
                     IILGMTFKTLPFIVWNKIYHQRSNKGKTPNPKDLFSNTIFKIMSIAYILGIVIFLLGL
                     VFENAILLKFGAFFILVTSILYNWNVLKIVYHKPTTT"
     gene            complement(1393..2202)
                     /locus_tag="PLU36_03565"
     CDS             complement(1393..2202)
                     /locus_tag="PLU36_03565"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HMZ45859.1"
                     /translation="MIKINANTKISALLKAHPQALDAIISISSKFNKLKNPLLRKLMA
                     SRTSIAMASKIGGVQVADFFEKFKSLGFEIDETIAEKKTNNESNLPIFIQHISKENIK
                     ELDVRPVINSGNDPFNIILNSVKQLQKGDVLKLINSFEPAPLIPILNKQGFEAFVEHE
                     SSEVVNTYFYKINETEINSPDLTNNEEDWQSIIEKYSNNLVTIDVRNLEMPLPMLTIL
                     EEIEKIPNYKALYVYHKRIPVFLLPELKERNFDYRTKEITDGEVHLLIFKA"
     gene            complement(2205..2528)
                     /locus_tag="PLU36_03570"
     CDS             complement(2205..2528)
                     /locus_tag="PLU36_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HMZ45860.1"
                     /translation="MQFKISQALPQCAEGKVKGFYGKDFINLQNGTVKLVRVDSFSKY
                     PLHLHPDKTEYAFVVKGNPFFVIDENKFTGEPGDFFIFPKNTKHAIENNTSNECVLLI
                     GAIIM"
     gene            complement(2530..3489)
                     /gene="ric"
                     /locus_tag="PLU36_03575"
     CDS             complement(2530..3489)
                     /gene="ric"
                     /locus_tag="PLU36_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HMZ45861.1"
                     /translation="METTVENILNVTLLEPRLKHPTIFARFDELNEGESLTIHNDHDP
                     KPLYYQLLGQRGNIFTWDYLENGPEWWKIKIQKRIIGEGNETLGEIAANDIRKAQIFK
                     KYGIDFCCGGKKTVKEVCAEKGLDINVIEKELAEADKVVASRPLPYNDWSLDFLADYI
                     VNTHHSYVKQTLPDLKNYAAKVAQVHGSAHPELIPIYELVDAVYAEMSTHMVKEEQIL
                     FPYIKEIVGAKNNGTPIQFSSMGSVRGPINMMEMEHESTGKCLEEIRTLSNNFTLPSD
                     ACASYTLLFKLLEEFEEDLHIHIHLENNILFPKALEIEKKLNN"
     gene            complement(3612..4577)
                     /locus_tag="PLU36_03580"
     CDS             complement(3612..4577)
                     /locus_tag="PLU36_03580"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HMZ45862.1"
                     /translation="MAAQKTYGIVLAVVIMLVAKFLAAYIPNLGVTILALMLGIIVRQ
                     LIPNFSKFTPGVVWSEKYVLETAIVFIGFGFEINKFSKIGTSTILMITVSIIVVILIS
                     LLLGKIFGSNNNKLYWLLGAGSAICGSSAIGATAPLIEAKEEETGLSLAVVNVLGLIG
                     MILLPLIAAAFQFSSVDTGIFLGGILQSVGHVVSAGFAVSDEVGQFSTVVKMGRVAFL
                     IPFLLIIFFLFRKRSNGTKIKLPLFILFFLIAVLISQLNIFNANAIKQITKLGDGLLN
                     IGMAAIGLKINIKSLWKISGKAFFAGGIIFIFQIILYFLFITVKL"
     gene            complement(4583..4963)
                     /locus_tag="PLU36_03585"
     CDS             complement(4583..4963)
                     /locus_tag="PLU36_03585"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMZ45863.1"
                     /translation="MKNFLLVFIISILLNACSAQDTAHYKVLSSSNYALALAQDKSAQ
                     LIDVRTPEEYEEGHLENAVNINIKDSNFIELVKKLNKEKPVYVYCRSGVRSRKAGAEL
                     EKLGFKQIFDLDGGILSWTGKTVK"
     gene            complement(4974..5279)
                     /gene="trxA"
                     /locus_tag="PLU36_03590"
     CDS             complement(4974..5279)
                     /gene="trxA"
                     /locus_tag="PLU36_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HMZ45864.1"
                     /translation="MQMTFQEIINQNKPILVDFFATWCGPCKTMAPILEEVKKRVGEK
                     ATILKIDVDKNPQTAAAYQISGVPTLILFKNGKPQWRQSGVISAKDIETIINNFYNH"
     gene            complement(5251..5466)
                     /locus_tag="PLU36_03595"
     CDS             complement(5251..5466)
                     /locus_tag="PLU36_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HMZ45865.1"
                     /translation="MNFIKKNMLAFIGIALGAIGGFLYWKLIGCNSGTCAITSKPLNS
                     TIYGAIMGGLVFSLFKKENANDVSRNN"
     gene            complement(5470..6021)
                     /locus_tag="PLU36_03600"
     CDS             complement(5470..6021)
                     /locus_tag="PLU36_03600"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HMZ45866.1"
                     /translation="MRYLFFMPFILLAACNNHSSNQIAVSNNQYLLKKGDSIATLAQK
                     ILLQKVSNAIELGGTDYAISFCNENALKLTDTLASINNVKLSRVTDRNRNSNNALKTT
                     TDTVAWNKMKIKQASVIETNNNGEVYFYKPIVMAMPTCLKCHGKETEITKSTLQLISE
                     KYPKDKALHYNAGELRGMWKIKM"
     gene            complement(6024..6266)
                     /locus_tag="PLU36_03605"
     CDS             complement(6024..6266)
                     /locus_tag="PLU36_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45867.1"
                     /translation="MIQKLLTGWNFMRLLRLALGIIIIIQGILVKDWMFILMGLLFSL
                     LPIFNIGCCGATACNTNSIITSKQKEESSFEEIKNK"
     gene            complement(6266..6862)
                     /locus_tag="PLU36_03610"
     CDS             complement(6266..6862)
                     /locus_tag="PLU36_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMZ45868.1"
                     /translation="MQENLNYLDKTFWNNRWLNNQTGWDIGYAAPAISNYFSQVDNKN
                     AAILIPGCGNAYEAEDLVKKGFNNITLVDIAPKAVEILQQKFINTPQVKVICTDFFEH
                     DGRYDYIVEQTFFCTFYPEVRKKYAAKAASLLNKNSKIIGLLFNKEFENNGPPFGGSE
                     LEYKKIFETEFILKTMSICTNSISPRHGSELFITLIKK"
     gene            complement(6869..7183)
                     /locus_tag="PLU36_03615"
     CDS             complement(6869..7183)
                     /locus_tag="PLU36_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMZ45869.1"
                     /translation="MGIFSMLFGSANKNKIADLCKQGALLVDVRTPAEFSTGSVKGAI
                     NIPLNTIHNQTAKFKNKNNIIVFCKSGIRSAQAKSILEQSGITNVTNGGTWQSVHNAI
                     NN"
     gene            complement(7190..8608)
                     /locus_tag="PLU36_03620"
     CDS             complement(7190..8608)
                     /locus_tag="PLU36_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ45870.1"
                     /translation="MFFQHVFDKSLAQSSYFIGCQATGEAIVIDAKRDIDTYLEIAQQ
                     NNLKITHITETHIHADFLCGSRELEAVTGARIYLSDEGGKDWQYEFKHIGLKDGDTIQ
                     VGNLTLNVLHTPGHTPESISFVLTDNPASNKPVMVFTGDFVFVGDIGRPDLLEKAAGI
                     IGTKEIGAKQMYQSLQKFTSLPDYVQVWSAHGAGSACGKALGAVHCSTVGYEKIRNWA
                     FQYNNKEQDFIDYLLADQPEPPKYFAMMKHLNKVNRRLLVVVPQQIKLTNSQFLSAYK
                     KGLKIIDTRNKQEFAKAHLPGSINIQNNNALSTWAGWFLNYQEQFILIADDNCIEEIT
                     RKLMRIGLDNVYGYISNINDLNVELHQVNNIQLSEFSELIKKDNVQIVDVRALTEYNA
                     AHIEKADHVFLGTIQNNLNKISRDKEVIIHCQAGDRSSTAYSILKSNGFHNIKNFSGG
                     MKSWLESKKNTVSYSNHVCKLN"
     gene            complement(8752..9375)
                     /locus_tag="PLU36_03625"
     CDS             complement(8752..9375)
                     /locus_tag="PLU36_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMZ45871.1"
                     /translation="MEELGQIEEFKSSPELVEKLYQHGIIKNYEAGSIILNEHSHIRS
                     IPIVTSGIIKVIRTDEEGREILLYYIKAGESCIMSFLGGMHNEASIVKAEVEEDAEIL
                     FLPIEKVSLFIKEYPQWLEYIFKLYHKRFEELLEIINAIAFKKVDERLLTLLHKLANL
                     TQSNIIAITHEQLANKLGTVRVVVSRLLKQLEEEGKVQLGRNKITLL"
     gene            complement(9402..9749)
                     /locus_tag="PLU36_03630"
     CDS             complement(9402..9749)
                     /locus_tag="PLU36_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45872.1"
                     /translation="MKLEHKQQNIIPFPKFLLRLSRYAIFAFSLILLSVLIGTLGYHH
                     FGKIGWLDSFHMACMILTGMGPVIEMQSVSAKIFSSLYALYSGIAFLSLTAVFFTPII
                     HRLLHILHIDQNK"
     gene            complement(9760..10734)
                     /locus_tag="PLU36_03635"
     CDS             complement(9760..10734)
                     /locus_tag="PLU36_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45873.1"
                     /translation="MTNTKRLFVLFYLISFICLSSCTSSKNINLSNQLSNSNCKSSIE
                     NAISNAPLPKPIYEIELDSLLTNKFSFAALNTANAIGVIDNLTMYARLKKDERTTPKL
                     ERRVVMLELLQKITQRINIASLEISAVASELDCEEERASQVATYLKSKESAKEKNLII
                     GSMIVGAVGSIAAEIISMNSNSGNTSNIVAITTSIIDVGLGTLIILNNKSIEFYHNRN
                     ALKDIWNNPKTSSFFTPSIWYYLTYENVSKNEKSLRKQLIEKWQDFGQITSSKNKDKN
                     IELYFGNGGKYETEQLKNRADMHDQIESYITLMKQDLKQLSIEIENLN"
     gene            complement(11105..>12395)
                     /locus_tag="PLU36_03640"
     CDS             complement(11105..>12395)
                     /locus_tag="PLU36_03640"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HMZ45874.1"
                     /translation="LKEAGFNTFLLPAEDVYIDLLTDSGTNAMSDQQWSAFMTGDEAY
                     AGSKSYYRMEKTIQDYYGYKYIIPTHQGRGAEHLLSKIMIKKGDYVPGNMYFTTTRLH
                     QELAGAKFVDVIIDEAHDPNNPFPFKGNVDINKLDALVKKVGASKIPYICVATNVNMA
                     GGQPISLANLKELRKYTKKHGIKIIHDMTRVAENAYFIQQREKGYQNTKIKDIVKEIC
                     SLTDGATMSAKKDALVNIGGFLAINDWDIYEEARNLVVAYEGLHTYGGIAGRDMEAIA
                     VGIVESLSDAHQKARVGQVEYLGAKMQEYGIPIVTPIGGHGIFVDAKAFLPHLTQDQF
                     PAQTLAAEVYLDSGVRTMERGIVSAGRKPNGDNYYPNLELVRFTLPRRVYTQAHMDVV
                     AESVAMVYENRTKIKGLKMIYEPKYLRFFQARFEQLK"
BASE COUNT         4100 a         2029 c         1731 g         4535 t
ORIGIN      
        1 agccaaggtc aactacattt aagcctattt caggatctat tacattgtac aaaccctcta
       61 ttgcaagttt acttttttct tcgttattag tttttaaatt catgttgttg ttggtttgtg
      121 ataaacaatt tttaaaacat tccaattata taaaatggaa gttactaaaa taaaaaaggc
      181 accaaatttt aataaaatag cattttcaaa aactaaacct aataaaaaaa ttacaatgcc
      241 taatatataa gctatactca ttattttaaa aatggtattg ctaaacaaat ctttagggtt
      301 aggcgtttta cctttattag aacgttgatg atatatttta ttccaaacaa taaatggaag
      361 tgttttaaag gtcataccta aaataatggc tgttatccat ccaaaaaata tcataaaacc
      421 ataagcaacc attggttgat aattttcggc agagctattg ataaataaaa taatgataat
      481 aaatagtagt agtattggta ataacattaa tactgttgaa agtaaagata gttttacttg
      541 ctcatctact tgttttctta ttctttgttt ataggcattg taacaataat aaataaacaa
      601 aactatggca acaaaaagta aataatctgg aattaatgta taagaaagag gaagataaaa
      661 gaagattatt ataaatgaaa taagggcggt attaattaac caaaaaataa tccataaaat
      721 tgtagggttt gtatattttg aaattaaaaa cataggaatt aatctggatg ctacgccaat
      781 aactaacaat aagaaccagc ccaaaatacc agtatgcaca tgcagaggta aatagtgcaa
      841 tgagttttgc ggcaaaaaat tataagtaaa attatatacc aatgctaacc ctaaactaac
      901 agtaataaat aaccaaataa ctgatgtgaa aacatacact gcatgaacat tttctttctt
      961 actttttgaa atactgtaac caatattaat tagataacac agaatggaaa gtagtactaa
     1021 tcttcctccc cattttgcag gagttcccat atcaaaaaca taaaaaccat aaaccaataa
     1081 tggaatacca atagcagata agataaatga aaggtgtgct aacttataac tatacaactc
     1141 tccttctatt aacactggta ctaattgatg gcttgccccc aaaataatca ttgtgcccca
     1201 acccaatgcc attatatgtg ttattgctaa aatgtgtggt tgaaaataat gggctaaaaa
     1261 ggtattcgaa gaaagcaata aaaatattga tgcaacaata aatgaaattg ctgcatacaa
     1321 ataaaaaggt aaaacaactt tataggaagt ggtttttaca atggatgaat tacctaagcc
     1381 aacgaacatt ttttatgctt taaaaattag taaatgtact tcgccatctg tgatttcttt
     1441 tgtacgataa tcaaaatttc gttctttaag ttcgggtaat aaaaaaacag gaattctttt
     1501 atggtaaaca tataaggctt tatagtttgg tattttttct atctcctcta aaatggtaag
     1561 cattggtaga ggcatttcta aattacgaac atcaatggtt actaaattat tagaatactt
     1621 ttcaataatg ctttgccaat cctcttcatt gtttgttaaa tctgggctgt taatttctgt
     1681 ttcatttatt ttataaaaat aagtattaac aacctcgctg ctttcgtgtt ctacaaatgc
     1741 ttcaaaaccc tgtttgttta aaattggaat taatggtgca ggttcaaaac tattaattag
     1801 ttttaaaaca tctccttttt gtaattgctt tactgagtta agaataatat taaaaggatc
     1861 gttaccacta ttaataaccg gacgaacatc taactcttta atattttctt tagaaatatg
     1921 ttgaatgaat attgggagat tgctttcgtt gttggttttt ttttctgcaa ttgtttcatc
     1981 tatttcaaac cctaaggatt taaatttttc gaaaaaatct gctacttgta caccaccaat
     2041 tttacttgcc attgcaatag atgtacgaga agccattagt tttcttaaaa gagggttttt
     2101 tagtttattg aatttagatg aaatactaat aatagcatct aatgcttgtg gatgtgcttt
     2161 taataaagca gaaatttttg tattggcgtt aatttttatc atttctacat aattattgca
     2221 ccaattaata aaacacattc attgctagta ttattttcta ttgcatgttt tgtgtttttg
     2281 gggaaaataa aaaaatcgcc aggctcgcca gtaaacttat tttcatcaat cacaaaaaaa
     2341 gggtttcctt ttacaacaaa tgcatattca gttttatctg gatgcaagtg taatggatat
     2401 tttgaaaatg agtcaaccct aactagtttt actgtaccat tttgtaaatt gataaagtct
     2461 ttaccataaa acccttttac tttaccctct gcacattgtg gcaaggcctg gctaatttta
     2521 aattgcattt tagttgttta attttttttc tatttctaat gctttaggaa aaagtatatt
     2581 gttttctaaa tgtatatgaa tgtgtaaatc ttcttcaaac tcttctaaca atttaaacaa
     2641 taaagtataa ctagcacaag catcgcttgg tagtgtaaaa ttattgctaa gtgttcttat
     2701 ttcttctaaa catttaccgg tagattcatg ctccatttcc atcatattaa ttggacctct
     2761 tacagaaccc atagagctaa actgaatagg ggttccatta ttttttgcac caactatttc
     2821 ttttatatat ggaaataaga tttgttcttc tttcaccatg tgggtactca tttctgcata
     2881 aacagcatct actaattcgt aaataggaat tagctcagga tgtgcacttc catgaacttg
     2941 tgcaactttt gcagcataat tttttaaatc gggtaatgtt tgttttacat aactatggtg
     3001 ggtgtttaca atataatctg ctaaaaagtc taagctccaa tcattataag gtaatggacg
     3061 agaggctaca accttatctg cttctgctaa ttctttttca ataacattta tatcaagccc
     3121 tttttctgca caaacttctt ttactgtttt tttaccacca cagcaaaagt ctattccata
     3181 ttttttaaat atttgagctt ttcttatatc attagcagca atctctccaa gggtttcatt
     3241 accctcgcct ataatgcgtt tttgaatttt tattttccac cattctggac cattttctaa
     3301 ataatcccaa gtaaaaatat tacctctctg ccccaataat tgataatata aaggctttgg
     3361 gtcatgatcg ttatgaatag ttaaactttc cccttcgtta agttcatcaa atcttgcaaa
     3421 aatagttggg tgctttaacc ttggctctaa cagggttaca tttagaatgt tttctacagt
     3481 tgtttccatc acattttttt tattttgtta ataatacagc aaaggtaaga gaattacaat
     3541 aaaagtaatt aaatactttt attattgaca aatgaatgac atttagaaga tgtagttaat
     3601 tgctcgcctt attataactt aactgttata aataaaaagt acaggataat ttgaaaaata
     3661 aaaataatac cgcctgcaaa aaatgctttg cctgaaattt tccataaaga tttgatgttt
     3721 attttaagtc caattgcagc cataccaata tttaataaac catcgcccag ttttgtaatt
     3781 tgttttatag cgttagcatt aaaaatatta agttgagaaa ttaaaacagc tattaagaag
     3841 aacaatataa ataatggaag tttaattttt gtaccattac ttctttttct gaataggaaa
     3901 aaaattataa gaagaaaagg aataagaaaa gctactctac ccatttttac aacagtagaa
     3961 aattgcccca cttcatcgct aactgcaaac cctgcactta ctacatgccc aacagattgt
     4021 agaatacctc ctaagaaaat accagtatca acagaagaaa actgaaaagc agcggcaatt
     4081 aatggaagca aaatcatacc aattaagcca agtacattta caacagctaa agataatccg
     4141 gtttcttctt cttttgcctc tattaatggg gcagttgctc caattgcaga ggagccacaa
     4201 attgcacttc ctgcacctaa aagccaataa agtttattgt tattgcttcc aaaaattttg
     4261 cctaataaaa gagaaattaa tataacaaca ataatgctta ctgtaatcat taatatggta
     4321 gaggtaccaa tttttgaaaa cttatttatt tcaaatccaa accctataaa aacaattgca
     4381 gtttctaata catatttttc tgaccataca acacctggtg taaatttaga aaagttggga
     4441 attaattgcc ttacaataat acctaacatt agtgcaagta tagtaacgcc caaattagga
     4501 atataagctg ctaaaaattt agcaacaagc attattacaa ctgctaaaac aattccataa
     4561 gttttttgtg ctgccaatat ttttacttta ctgtttttcc agtccacgat aaaataccac
     4621 cgtctaaatc aaaaatttgc ttaaagccca atttttctaa ttcagctcca gcttttctac
     4681 ttcttacacc acttctgcaa tacacataaa caggcttttc tttgtttagt tttttaacta
     4741 attctataaa gttactatct tttatattga tgtttacagc attttctaaa tgcccttctt
     4801 catattcttc tggtgttcta acatctatta attgtgccga tttatcttgt gctaaggcta
     4861 gtgcataatt actactagat aacactttat aatgtgctgt atcttgtgca ctacaagcat
     4921 ttaataagat actaattata aataccaaca aaaagttttt catgtaaaaa ggtttaatgg
     4981 ttgtagaaat tatttattat tgtttctata tcttttgcag aaataacacc cgattgccgc
     5041 cattgtggct taccattttt aaacaaaatt aatgttggta caccactaat ttgataagca
     5101 gcagcagttt gtggattttt atctacatca atttttaaaa tagtagcttt ttcgccaaca
     5161 cgttttttta cctcttctaa aataggagcc attgttttac atgggccaca ccaagttgca
     5221 aaaaaatcta ccaatatagg tttgttttga ttaattattt cttgaaacgt catttgcatt
     5281 ttctttttta aataaagaaa aaaccaagcc tcccattatt gcaccataaa tagtgctgtt
     5341 tagtggttta gaggttatgg cacatgtacc agaattacag cctataagtt tccaatataa
     5401 aaacccgcca attgctccta atgcaatacc aataaaggca agcatatttt ttttaataaa
     5461 attcataatt tacattttta ttttccacat accccttaac tctcctgcat tataatgtaa
     5521 tgctttatct tttggatatt tttcagatat aagttgtagg gtagattttg ttatttcagt
     5581 ttctttacca tggcacttta aacaagtggg catcgccatc actattggtt tataaaaata
     5641 aacttctcca ttgttgttgg tttcaataac actggcttgt ttaattttca ttttattcca
     5701 ggctacagta tcggtagtag tttttaatgc attgttacta tttctgtttc tatcggtaac
     5761 cctgctaagt tttacattat ttattgatgc taaagtgtcg gttaatttta aagcattttc
     5821 gttgcaaaaa cttatggcat aatctgtacc tcctaattca atagcattac ttactttttg
     5881 taataatatt ttttgagcta gagtagcaat gctatctccc ttctttaata agtattgatt
     5941 attggataca gctatttgat tggaagagtg attattgcaa gcagccaaca aaataaatgg
     6001 cataaaaaat aaatacctca tgtttattta tttttaattt cttcaaaact tgactcttct
     6061 ttttgtttac tagttattat actattggta ttgcaggcag ttgccccaca acaaccaata
     6121 ttaaatatgg gtagtaatga aaataataaa cccattaaaa taaacatcca atctttaact
     6181 aaaatgcctt gtataataat aataatacca agtgctaatc ttaataatcg cataaaattc
     6241 caaccagtta aaagcttttg tatcattatt tttttattaa tgttataaaa agttcactac
     6301 catgtcttgg ggaaatgcta ttggtgcaaa tgctcattgt ttttaaaata aattctgttt
     6361 caaaaatttt tttatactcc aattcacttc ctccaaatgg agggccattg ttttcaaatt
     6421 ctttattaaa aagcagccct attattttac tgtttttgtt taataatgaa gcggctttgg
     6481 cagcatattt ttttcttact tcgggataaa aggtacaaaa aaaagtttgt tcaactatat
     6541 aatcgtatct gccgtcatgc tcaaaaaaat cagtgcaaat tactttaacc tgtggagtgt
     6601 ttataaattt ttgctgaagt atttctacag cctttggagc aatatctaca agcgtaatat
     6661 tattaaaccc tttttttacc aaatcttctg cttcgtaggc attgccacaa cctggaatta
     6721 gaattgcagc gtttttatta tctacctggc taaaataatt acttattgca ggggctgcat
     6781 aaccaatatc ccagcctgtt tggttgttta gccaacggtt attccaaaag gttttatcta
     6841 aatagtttaa attttcttgc acaattattt aattgtttat agcattgtgt acgctttgcc
     6901 atgttccacc attggtaaca tttgttatac cactttgctc taaaatgctt ttagcctgag
     6961 cactgcgtat gccactttta caaaaaacaa taatattgtt tttattttta aattttgcag
     7021 tttggttatg aatagtattt aaaggaatgt ttattgcacc tttaacactg ccagtcgaaa
     7081 actctgcagg tgtacgaaca tcaaccaata aagcaccttg tttacaaagg tctgcaattt
     7141 tatttttatt tgcagagcca aaaagcattg aaaaaattcc catattatat taatttaatt
     7201 tacaaacatg attactataa ctaacggtat tttttttact ctctaaccaa cttttcatac
     7261 cgcccgaaaa gttttttata ttgtgaaagc cattactttt taatatagaa taagcagtag
     7321 aagatctatc gcctgcctgg caatgaataa ttacttcttt atctctgctt atcttgttta
     7381 aattattttg aattgtgccc aaaaaaacat gatctgcttt ttcaatatgt gctgcattat
     7441 attctgttaa tgctctaaca tctacaattt gtacattatc ctttttaatc aattccgaaa
     7501 attcgctaag ctgtatgtta ttaacttgat gcagctcaac atttaaatcg tttatattgc
     7561 ttatatagcc atatacatta tctaaaccaa ttctcattaa ttttctggta atttcctcta
     7621 tacaattatc atctgctatt aaaataaact gttcttgata atttaaaaac caaccagccc
     7681 aagtagaaag ggcattgttg ttttgaatgt taatactacc aggaaggtga gcttttgcaa
     7741 actcttgttt atttcttgta tcaataattt ttaatccctt tttatatgca gaaagaaact
     7801 gactgttggt taattttatt tgttgaggca ctaccaccaa aagcctacga tttactttat
     7861 tcaaatgctt catcatagca aaatattttg gaggctctgg ttggtcggct aataaataat
     7921 caataaaatc ttgctcttta ttattgtatt gaaaagccca gttgcgaatt ttttcgtaac
     7981 caacagtaga gcaatgtact gcccctaatg ctttgccaca ggcagagcct gcaccatgag
     8041 cagaccaaac ttgcacataa tcgggcagag aagtaaattt ttgtagtgat tgatacattt
     8101 gcttggctcc tatttcttta gtgcctatta tgcctgctgc cttttctaat aaatctggtc
     8161 ttccaatatc gccaacaaaa acaaaatctc ctgtaaaaac cattacaggt ttattgcttg
     8221 caggattatc ggttaaaaca aatgaaatac tttctggtgt atggcctggt gtgtgtaaca
     8281 catttaaagt taaattacct acttgaatag tatcgccatc ttttaagcca atatgtttaa
     8341 attcatattg ccagtcttta ccgccctcgt cagataaata tattcttgca ccagtaactg
     8401 cttctaattc tcttgagcca caaagaaaat cggcatgaat atgtgtttct gttatatgtg
     8461 ttatttttaa attattttgt tgggcaattt ctaaataggt atcaatatct ctttttgcat
     8521 ctattacaat tgcttctcct gtagcttggc agccaataaa gtagctactt tgtgcaaggc
     8581 ttttatcaaa tacatgttga aaaaacatag cgaaatattt taagacacca aagatgaata
     8641 tatttaaaaa tatagttggt tacttttgtt acactatgca atgttttaca tttctttaat
     8701 aaaatgaatc tttaaataaa taagttttaa ggaataaaaa acaagcagca actataataa
     8761 agtaatttta ttgcggccta attgcacttt tccttcttct tctaattgct ttaataatct
     8821 tgaaacaaca acacgaacag tacctaattt atttgctagt tgttcgtgag ttatggctat
     8881 aatattagat tgagtaagat ttgctaattt gtggagcagg gttaacaacc tttcatctac
     8941 ctttttaaaa gctattgcat ttataatttc taataattct tcaaaacgtt tgtgatacaa
     9001 tttaaaaata tactctaacc attgaggata ttctttaata aacaacgaaa ctttttcaat
     9061 aggtaaaaaa agtatttcag catcttcttc tacttctgct tttactatac ttgcctcatt
     9121 gtgcatgcca cctaaaaaag acatgataca actttcgcca gcttttatat aatacaataa
     9181 aatttctctt ccttcttcat ctgttctaat cacttttata attccagatg ttacaatagg
     9241 tatagaccta atgtgtgaat gttcatttaa tattatacta cctgcttcat aattttttat
     9301 aataccatgt tggtataatt tttcaaccaa ttctggagac gatttgaatt cttctatttg
     9361 ccctaattct tccattgtaa atattttatg tgctataaag tttatttatt ttgatctata
     9421 tgaagtatat gcaaaagcct atgaattatt ggtgtaaaga aaacagcagt taagcttaaa
     9481 aatgcaatac cactatataa tgcatataat gatgaaaaaa ttttagcaga taccgattgc
     9541 atttctatta ctggccccat gcctgttaat atcatacaag ccatgtgaaa actatctaac
     9601 caaccaattt ttccgaaatg atgatagcca agtgtgccaa ttaaaacaga taacaatatt
     9661 agtgaaaaag caaaaattgc atatcggctc aatcgtaata aaaacttagg aaaaggaata
     9721 atattttgtt gtttgtgttc aagtttcatt tttaaaatat taatttaagt tttcaatttc
     9781 tattgaaagt tgttttaaat cttgtttcat tagggttata taagattcaa tttggtcatg
     9841 catatctgca cggtttttta attgttccgt ctcatatttt cctccattac caaagtaaag
     9901 ttctatattt ttatctttat ttttggatga agttatttgg ccaaaatctt gccatttttc
     9961 tatcaactgt ttacgtaaag atttttcgtt tttagataca ttttcataag ttaaataata
    10021 ccaaatggaa ggtgtaaaaa aagaagatgt ttttggattg ttccaaatgt cttttaatgc
    10081 atttctatta tgataaaatt ctatactttt attgtttaaa attataagtg tgcctaagcc
    10141 aacatctatt atactggttg taattgcaac aatatttgag gtgttaccac tatttgaatt
    10201 cattgaaatt atttctgcag caatagagcc tactgctcca acaatcatag agccaatgat
    10261 taaatttttt tctttggcac tttctttact tttcaaatag gtggctactt ggcttgccct
    10321 ttcttcttca caatctaatt cgcttgccac tgccgaaatt tctaaagagg ctatatttat
    10381 acgttgtgta attttttgca acaattctaa cataaccact cttctttcta attttggtgt
    10441 tgttctttcg tcttttttta acctggcata catagttaaa ttatcaataa cacctatggc
    10501 gttggctgta tttaaggcgg caaaagaaaa cttattggta agcaagctat ctagttcaat
    10561 ttcataaatg ggtttaggta agggggcgtt agaaatggca ttttctatag aacttttaca
    10621 attgctattg cttagttggt tgcttagatt aatattttta gaagaggtgc aagaagaaag
    10681 gcagataaaa gaaataaggt agaataatac aaatagcctt tttgtgttgg tcatatttta
    10741 gtgcttttat caaagttaac atactagttt taataatact acaataagct ctaataatga
    10801 agttgaatta aataaataaa aactaaaaat aaaatagaaa acttttagga atggaattgt
    10861 agggaaaaga aatatttttt tataaaaata atgtaccaga aaaaaggcta ggatatttta
    10921 atggagggag attgaaagtt gttactttat agctaaagtt gaaaagccat tgcattgtaa
    10981 tactctctat attgaagagt tgctcttctt aattttattt tttaaatttc taataatagc
    11041 aaccaatttc ataaaaaaag gagccaaatt aattgactcc ttttcaatat aaaatagaaa
    11101 ggtattattt taattgttca aaacgggctt ggaaaaagcg aagatactta ggctcataaa
    11161 tcatttttaa gccttttatt tttgttctgt tttcgtaaac cattgctaca gattctgcta
    11221 ctacatccat gtgggcttgt gtataaaccc ttctgggtag tgtaaaacga actaattcta
    11281 aattaggata ataattatcg ccatttggtt ttcttccagc agatacaata ccacgttcca
    11341 ttgttctaac accagagtct aaatacactt cagcagctaa tgtttgagca ggaaattggt
    11401 cttgggttag gtgaggtaaa aatgcttttg catctacaaa tattccatga ccaccaattg
    11461 gagttacaat aggaataccg tactcttgca ttttagcacc taaatattct acttggccta
    11521 cacgagcttt ttggtgtgca tcgctaaggc tttctacaat acctacagca attgcttcca
    11581 tatctcttcc tgcaatacca ccataagtgt gtaacccttc atatgctaca acaaggtttc
    11641 tggcttcttc ataaatatcc caatcgttaa ttgctaaaaa gcctccaata tttacaaggg
    11701 catctttttt agcactcatt gttgcaccat ctgttaaaga gcaaatttct tttactatgt
    11761 cttttatttt ggtattttga taaccctttt ctctttgttg tataaagtaa gcattttctg
    11821 ctacacgggt catatcgtgt attattttta taccatgctt tttagtatat tttcttaact
    11881 cttttaaatt tgctaaacta ataggttgcc cacctgccat gtttacattg gtagcaacac
    11941 atatataagg tattttagat gctcctactt ttttaactaa tgcatctaat ttatttatat
    12001 caacattgcc cttaaaagga aaaggattat tggggtcgtg ggcttcatct ataattacat
    12061 ctacaaactt ggcacctgct aactcttgat gaagtcttgt ggtggtaaag tacatattac
    12121 ctggtacata atcgcctttt ttaatcatta ttttagatag taaatgctct gcacctctgc
    12181 cttggtgtgt tggaattatg tatttatacc cataataatc ttgaatggtt ttttccattc
    12241 tataatagct tttgctgcca gcataagcct catcgccagt cataaaagcc gaccattgtt
    12301 ggtcgctcat tgcatttgtt ccactatcag ttaacaaatc aatatataca tcttctgcag
    12361 gaagaagaaa tgtattaaaa ccagcttctt ttaaa
//
LOCUS       DAOQPK010000045        12361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_2618_length_12361_cov_4.910206, whole genome shotgun sequence.
ACCESSION   DAOQPK010000045 DAOQPK010000000
VERSION     DAOQPK010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 12361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12361
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2618_length_12361_cov_4.910206"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="PLU36_03645"
     CDS             complement(<1..959)
                     /locus_tag="PLU36_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45875.1"
                     /translation="MKKFLSLLFMLFFFMITSNAQEKDQTDIREMYVRIAVVKKINND
                     SVIALIDGGKRYGIKNGSVGIVKGVYKSGDDRSDFEIGYASVYKVTDTTAFVTIKPAD
                     KLGTKKGYDIRKGDYVILKVNVPKLTYRSIFFELALLDIQFNNLYNNSLYSIADLFYR
                     DSKQLEDSLLIAGSKDVFETYDALKNDTSFKSLNEPLTEGRYKGRSIFGVMKDCNAND
                     VFSFLHFVSNYPGKYIGHTWKLNETFATWVLNKAPYSKKEIYDSIMSYKNKPALLKVF
                     ITKNIALIKQKGFVTDWISDAATALNLKQTQKALNFFEAAKLV"
     gene            complement(1211..1795)
                     /locus_tag="PLU36_03650"
     CDS             complement(1211..1795)
                     /locus_tag="PLU36_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HMZ45876.1"
                     /translation="MKTVTIEGQLRTEFGKKATRQIRSQEQVPAVIYGGAQEVNFAAS
                     LKSFKPLVYTPEFQLAEIKVGEKTYKCILKDLQFDKVTDELIHIDLLELVDDKKVIAN
                     LPLKYTGNSIGVKDGGKLVLKMKSVKVKTYPKYLKENLEVDITNLNINGNLRVEDIKA
                     DNIEIMNSPRIPIASVVMTRQLKQEEATKDEKKK"
     gene            complement(1858..2628)
                     /locus_tag="PLU36_03655"
     CDS             complement(1858..2628)
                     /locus_tag="PLU36_03655"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HMZ45877.1"
                     /translation="MKTKKDIINNWLPRYTGEDLENFGEYILLTNFSNYVELFAQWNK
                     VKVIGKDKPFQCATANNISIINFGMGSPGAATIMDLLAAINPKAVLFLGKCGGLKKRN
                     KIGDLILPIAAIRGEGTSNDYFPPEVPALPAFALQKAISTTIRDFKTDYWTGTVYTTN
                     RRVWEHDEAFKAYLTKIRVYAIDMETATVFTVGFYNKIPTGALLLVSDNPMIPEGVKT
                     AASDKKVTTLFVERHLKVGIESLNQLKNNGLTVRHLKF"
     gene            2732..3181
                     /locus_tag="PLU36_03660"
     CDS             2732..3181
                     /locus_tag="PLU36_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HMZ45878.1"
                     /translation="MIKINYPTYQFKFNQKNGKEFIFDAFRKLWVRLTPEEWVRQNFL
                     QYLVQEKKYPIACIAVEKEVMVNGIKKRCDIIVFKNYTPYMIVECKEMNVALTEKTIQ
                     QILTYNQSLSVNILVITNGNNTFALETTTKKSLNSIPAFSETIINDF"
     gene            3227..4903
                     /gene="hutU"
                     /locus_tag="PLU36_03665"
     CDS             3227..4903
                     /gene="hutU"
                     /locus_tag="PLU36_03665"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF003820.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HMZ45879.1"
                     /translation="MSTYQRYDENKYKTPTGSSLNCKGWIQEAALRMLLNNLNPEVAE
                     RPDDLIVYGGRGKAARNFESLDLIIKALKNLENNETLLIQSGKPVAVLTTHADAPRVL
                     ISNSQLVPNWANWKHFTELEQKGLMMYGQMTAGSWIYIGSQGIVQGTYETYAAAADKQ
                     FGGTLKGTLNVTAGLGGMGGAQPLAITMNEGVALVAEVEEWRIDKRIETKYLDEKYTD
                     IDKAIDAALEYKKQSVAKSIGVLCNAVHLLQKLNERNIIPDTLTDQTSAHDPLIGYVP
                     HTLTNEQANELREKNAEEYIKLSYESMYLHVQLMLQLQQKGAITFDYGNNIRARAQEH
                     GLKNAFDFPGFVPAYIRPLFCEGKGPFRWAALSGDPSDIAVTDELIMQMFPDNKGLMR
                     WMKMAKEKIAFQGLPARICWLGQGEREKAGLAFNELVRTGKVKAPIVIGRDHLDTGSV
                     ASPNRETEAMLDGSDAVADWPILNALVNTAGGASWVSLHHGGGVGMGYSIHAGMVIVA
                     DGTEEATKRLSRVLRNDPGMGVIRHADAGYELAKQTAQEHGLDLVAKQNS"
     gene            4912..5325
                     /locus_tag="PLU36_03670"
     CDS             4912..5325
                     /locus_tag="PLU36_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004869 - cysteine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystatin domain-containing protein"
                     /protein_id="HMZ45880.1"
                     /translation="MPYYNTEESIQHVVTLLNKRLYNAGYSFRVIEVSEYTVYVQASF
                     DFGYYVNVHIQFDNCIFTNLKERDEWPDAWHADQLFLLDEVEVENILNWYDVEKEPTN
                     NLFAFIFNINGCNINNAGVVVCEKLYIRWEHPYYD"
     gene            5405..5617
                     /locus_tag="PLU36_03675"
     CDS             5405..5617
                     /locus_tag="PLU36_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45881.1"
                     /translation="MEQQHKLAEEQNKQDFTKSWVSSSRFLFYLLITCMMASTIGGCF
                     KLYKSKYKGTPEYEIQGSTKYTPDYK"
     gene            5690..5762
                     /locus_tag="PLU36_03680"
     tRNA            5690..5762
                     /locus_tag="PLU36_03680"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5723..5725,aa:Gly,seq:gcc)
     gene            5794..5879
                     /locus_tag="PLU36_03685"
     tRNA            5794..5879
                     /locus_tag="PLU36_03685"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5828..5830,aa:Leu,seq:taa)
     gene            complement(5928..6587)
                     /locus_tag="PLU36_03690"
     CDS             complement(5928..6587)
                     /locus_tag="PLU36_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HMZ45882.1"
                     /translation="MPELKQIISLIKKDILLEVRQQYSFYGIILYVASTIFVIYLTMG
                     QPEEQIWNGLFWIVQLFIVTNAVAKSFLSESKGKMIYYYTIAGAKVFILSKLIFNLLL
                     MLLMSLISLLLFMVLLNNPMHNAMLFVGITILGGTSLSLVFTFLSAIAAKAMQQAALM
                     AILGFPLIIPQLLLLMKITTPAFSNVIQESWGQLILMLIALDVMVVLLAIILFPFLWK
                     D"
     gene            complement(6603..7166)
                     /locus_tag="PLU36_03695"
     CDS             complement(6603..7166)
                     /locus_tag="PLU36_03695"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HMZ45883.1"
                     /translation="MFIEPNLSGERRGWIEVICGSMFSGKTEELIRRLKRAKIANLKV
                     EIFKPGIDVRYDETKVVSHDANAIQSTPVDNSQKILLLTQDVDVVGIDEAQFFDDEIT
                     NVCETLATKGIRVIVAGLDMDYLGKPFGQMPNLLAIADYITKLHAICVQCGNIANISY
                     RKIEQDGQVLLGEKDVYEPRCRVCYHK"
     gene            7253..7948
                     /locus_tag="PLU36_03700"
     CDS             7253..7948
                     /locus_tag="PLU36_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45884.1"
                     /translation="MERVGTLINKLKEQLDQKQDAAKLLLTAQMLLAELQQQQPSSNS
                     GKVSVTMPSVTFVSEIIAEVKNESKPKAVEQPKKAPQNENQTGWLFNPELASIPTLVH
                     QEIVTNEVVDKVEDKKELFELKDTLVTEEEPSLNDILKEEKTEIATVLKGAPIKDLKK
                     AIGINDRYVFINELFRGDEVMYERSIKTINTFNIYAEAEYWIQRELKVKLGWEENNST
                     VESFNQLVKRRFA"
     gene            complement(7921..9180)
                     /locus_tag="PLU36_03705"
     CDS             complement(7921..9180)
                     /locus_tag="PLU36_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HMZ45885.1"
                     /translation="MKIFYNIFIAVYPFIAKIISTKNTKAKLWLTGRKNIFTKLKEAF
                     ANNKSEVIWMHCSSLGEFEQGRPVLEKIKQTYPSYTILLTFFSPSGYEIQKNYAYANW
                     VYYLPLDSAKNATQFLDIVQPKLTLFIKYEFWYYYLTEIKKRNIPLLLLSASFRKNQP
                     FFKWYGKIYREMLQCFSYFFVQDNNCADLLHKIGFTKNVSIAGDTRFDRVIEIANSFE
                     SIEIIEKFIGTDKVIVAGSTWTDDDEALDHYANTNTHIKFIIAPHQIETERVNECLKL
                     YKKAILFSVLVNEPNSNFTEKNVLIIDNIGMLSKLYKYATICFVGGAFGDDGVHNVLE
                     AAVYKKPVVFGPEFEKYIEAKELVENNGGISIENALELEQVFNKLLTDNLFYETTAIN
                     AENYVQNNSGATKIIVDFIYAKRLLTN"
     gene            9304..10437
                     /locus_tag="PLU36_03710"
     CDS             9304..10437
                     /locus_tag="PLU36_03710"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HMZ45886.1"
                     /translation="MKPIQMVDTKTQYLKIKEEVDKGIFEVLESAQYIGGAKVNEFSE
                     ALNVYHNSAFTITCANGTDALQIALMALDLQPGDEVITPSFTYIATTEVVALLRLKPV
                     FVEVNPKTFCIEPDAIRKAITPKTKAIVPVHLYGHAAPMEEIMQIAKEYNLYVVEDNA
                     QAIGCNYTFNNGTTKKTATIGHIGCTSFYPSKNLGAYGDGGAIFTQDAMLAEKLKMIA
                     NHGQKTRYIHDIVGCNSRLDAIQAAILTVKLKQLDNYIVARNNAANYYNKAFANHTKI
                     TTPFVASYSNHVYHQYTLILDGVNRDGLVNYLAEKKIPSMIYYPIPGHKQKVFETIGS
                     EKYNLEITDWLTEKVISLPIHTELETDQLEYITTEVLNYINNH"
     gene            10445..11746
                     /locus_tag="PLU36_03715"
     CDS             10445..11746
                     /locus_tag="PLU36_03715"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HMZ45887.1"
                     /translation="MKIAVVGTGYVGLVSGTCFAETGNTVTCIDIDQNKVSKLSNGEI
                     TIYEPGLEKIFLRNLKENRLKFTTNLAEGIKDAAIVFLALPTPPGEDGSADLKYVLGV
                     ANDLGKIITDYKVIVDKSTVPVGTAAKVHAAIAANAKVEFDVVSNPEFLREGVAVDDF
                     MKPDRVVIGTNSDRAKKVMADLYAPYVRQGNPIIFMDEKSAELTKYAANSFLATKISF
                     MNEIAQLCEKLGADVDMVRKGIGSDDRIGKRFLFPGIGYGGSCFPKDVQALIKSSDEV
                     NYNFQILKAVEEVNEAQKLHLIPKIKKYFNNNLQGKHFALWGLAFKPNTDDIREAPAL
                     YVIDALVNAGATVTAYDPEAIKNVSNLIGNKIAYADNQYAALQNADALIIATEWSEFR
                     TPDFDRVAGTLKNKIIFDGRNLFDVQQIREMGYYYESIGRP"
     gene            11775..>12361
                     /locus_tag="PLU36_03720"
     CDS             11775..>12361
                     /locus_tag="PLU36_03720"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HMZ45888.1"
                     /translation="MRKKVLITGAAGFLGSHLCDRFIKEDYHVIAMDNLITGDLKNIE
                     HLFPLENFEFYNHDVTKYIHISGNLDYILHFASPASPIDYLKIPIQTLKVGALGTHNC
                     LGLAKEKKARILVASTSEVYGDPLVHPQTEEYWGNVNPVGPRGVYDEAKRYMESITMA
                     YHTFHNVETRIVRIFNTYGPRMRLNDGRALPAFVG"
BASE COUNT         4388 a         1857 c         2008 g         4108 t
ORIGIN      
        1 aaaactaatt ttgcagcctc aaaaaaattc aaagcttttt gtgtttgttt taaatttaaa
       61 gcagtagcag catcacttat ccaatctgtt acaaaccctt tttgttttat aagagcaatg
      121 tttttagtta taaatacttt taaaagtgct ggtttgtttt tataactcat tatactatca
      181 taaatctctt ttttgctata aggtgctttg tttaaaaccc atgtagcaaa ggtttcattt
      241 agtttccatg tgtgaccaat atattttcct gggtaattac ttacaaaatg caaaaaacta
      301 aatacatcat ttgcattaca gtctttcatt acaccaaaaa tacttcttcc tttataccta
      361 ccttctgtta aaggttcatt taggctctta aaactagtat cattttttaa ggcatcatag
      421 gtttcaaata catctttaga accggctatt aataagctat cttctagttg tttactatct
      481 ctatagaaaa gatcagcaat gctgtataaa ctattgttgt ataaattatt aaactgaata
      541 tctaataaag ctagttcaaa aaaaatgctt ctgtaggtta gttttggtac attaactttt
      601 aaaattacat aatcgccttt tcttatatca tacccctttt tagtgccaag tttatcagct
      661 ggtttaatag ttacaaaggc agtagtatct gttactttat atacactagc ataaccaatt
      721 tcaaaatcgc ttctatcgtc cccagatttg tacactcctt tcactattcc tacacttcca
      781 tttttaatac catatctttt gccaccatca attaaagcta ttacactatc attatttatt
      841 ttttttacaa ctgcaatgcg tacatacatt tctctaatat ctgtttgatc tttttcttgt
      901 gcattgcttg ttatcatgaa aaaaaacaac atgaaaagaa gagagaggaa ctttttcata
      961 tattagttat tttgaattaa gaaggatgag tgtatagtat taaaatataa taggtatagt
     1021 taatttattt tctgatatac tgtttcaata ttattttgat atttaaagtt atataattta
     1081 aaacaattgc ttgtaccgct ttttgggtat tttatttatg atgataaaga ataactttaa
     1141 actaacagag taaataaaat aaaaaaccct tgaaataatc caagggtttg aaaaattatt
     1201 taaaatataa ttattttttc ttttcatctt tagttgcttc ctcttgtttt aactgacgag
     1261 tcattacaac agaagcaata ggaatacgtg gagagttcat aatttcaata ttatctgctt
     1321 taatatcttc cacacgtaag ttgccattaa tgttaagatt ggtaatatct acttctaaat
     1381 tttctttcaa atatttaggg taagttttta ccttaaccga tttcattttt aatactaatt
     1441 tacctccatc ttttacacca attgaattac cagtatattt taatggtaaa ttggcaatca
     1501 cttttttgtc atctacaagt tctaacaaat caatatgtat taattcatcg gtaaccttat
     1561 caaactgtaa atcttttaaa atacatttgt aagttttttc accaacttta atttcggcta
     1621 actgaaattc aggtgtataa accaatggtt taaaagattt taaagaagca gcaaagttta
     1681 cttcttgtgc acccccgtaa attacagcag gcacttgctc ctgagagcgt atttggcggg
     1741 tagctttttt gccaaattcg gtcctcagtt gtccttcaat tgtaactgtt ttcattataa
     1801 tgattttttt ttggacggca aaggtaaggg taaacaatat aattatgcta acctacttta
     1861 aaatttaaga tgtcttacag ttagtccgtt gtttttcaac tggtttagcg attcaatacc
     1921 tacttttaaa tgcctttcta caaataaagt agtaactttt ttatcgcttg cagccgtttt
     1981 taccccctct ggtatcattg ggttatcact aactaataaa agagctccag taggtatttt
     2041 attataaaaa ccaacagtga aaaccgttgc agtttccata tctatggcat acactctaat
     2101 tttagttaag taagctttaa aagcttcgtc gtgttcccaa actcttctat tggtagtata
     2161 aacagtacca gtccaataat cagtcttaaa atcacgaatg gttgtagaaa tagctttctg
     2221 taatgcaaaa gcaggaagtg ctggtacttc aggagggaaa taatcgttgg atgtaccttc
     2281 tcctctaata gcagcaattg gtagtattaa atcaccaatt ttatttcttt ttttcaagcc
     2341 gccacatttt cctaaaaaaa gcacagcttt tgggtttatt gctgcaagta aatccataat
     2401 agttgcagca ccagggctac ccattccaaa attaataata cttatattat tagctgttgc
     2461 acattgaaaa ggtttatcct tacctataac ttttacttta ttccattgag caaatagttc
     2521 tacataatta ctaaaattgg ttaatagaat atattctcca aaattttcta aatcttctcc
     2581 agtgtatcta ggaagccagt tgttgataat gtcttttttt gttttcatac ttttattctt
     2641 caagttttat tttcttcttt taaattatgt accagccaac ttatatttgg caaatagcat
     2701 ttgtaaatat aggcaagaat ttccttaaac gatgataaaa ataaattacc ccacctatca
     2761 atttaaattc aaccaaaaaa atgggaagga gtttattttt gatgctttta gaaaactttg
     2821 ggtaaggctt acaccagaag aatgggttag acaaaatttt ttgcaatacc ttgttcaaga
     2881 aaaaaaatac cctatagcat gtattgctgt tgaaaaagaa gtgatggtaa atggcataaa
     2941 aaaacgatgc gatattatag ttttcaaaaa ctatacacct tacatgattg ttgaatgtaa
     3001 agaaatgaat gttgccttaa ccgaaaaaac aattcagcaa atacttactt ataaccaatc
     3061 attatctgta aatatattgg taataaccaa tggcaataat acatttgcat tagaaaccac
     3121 aacaaaaaaa tcacttaatt caattccagc attttctgaa acaataataa atgatttttg
     3181 aagttggctt ctttaacctc ttaacaaaaa ttaataatta acttttatga gtacttatca
     3241 acgttatgat gaaaataaat acaaaacacc aacaggtagt agcttaaact gcaaaggatg
     3301 gatacaggaa gcagcgttaa gaatgttgtt aaataattta aaccccgagg ttgctgaaag
     3361 accagatgat ttaattgttt atggaggaag gggcaaagct gccagaaatt ttgagtcgtt
     3421 agatttaatt ataaaagcat taaaaaattt agaaaataac gaaacattac ttatacaaag
     3481 tggtaagcct gtagctgtac ttaccacaca tgcagatgca ccacgtgttt taatcagcaa
     3541 ttctcaatta gtacccaatt gggcaaattg gaagcacttt actgaattag aacaaaaagg
     3601 attaatgatg tatgggcaaa tgactgctgg tagttggatt tatataggaa gccaaggtat
     3661 tgtacaaggc acttatgaga cttatgcagc agctgctgat aaacagtttg gaggaacact
     3721 taaaggaact ttaaatgtta cagcaggttt aggaggaatg ggaggtgcac agcctttagc
     3781 cattacaatg aatgagggtg ttgctttagt tgccgaagta gaagagtgga gaatagataa
     3841 aagaattgaa acgaaatatt tagacgaaaa atatacagat atagataaag caatagatgc
     3901 agcacttgaa tataaaaaac aaagtgtagc aaaaagtatt ggcgtacttt gtaatgctgt
     3961 tcatttgtta caaaaattaa atgaaagaaa tattataccc gatactttaa cagaccaaac
     4021 atctgcccat gatccattaa taggttatgt gccacatact ttaacaaacg aacaagcaaa
     4081 tgaattaaga gaaaaaaatg cagaggagta tattaaactt agctacgaaa gtatgtattt
     4141 acatgtacaa ctaatgttgc aattacaaca aaaaggtgca ataacatttg attatggtaa
     4201 caatataaga gcaagagctc aagagcatgg tttaaaaaat gcctttgatt ttcctggatt
     4261 tgtgccagct tatatacgtc cattattttg tgaaggaaaa gggcctttta gatgggctgc
     4321 tttaagtgga gacccatcag atattgcagt aaccgatgaa ttaattatgc aaatgtttcc
     4381 tgataacaaa ggattaatgc gttggatgaa aatggcaaaa gaaaaaattg cttttcaagg
     4441 attacctgca agaatttgtt ggttaggcca gggcgaaaga gaaaaagcag gattggcatt
     4501 taatgaactt gttagaacag gaaaagtaaa agcacccatt gttattggta gagaccattt
     4561 agatacaggt agtgttgcta gccctaacag agaaactgaa gcaatgttag atggaagtga
     4621 tgctgtagca gattggccaa tattaaatgc attggtaaat actgctggtg gtgctagttg
     4681 ggtaagttta catcatggtg gtggggttgg tatgggttat agtattcatg caggtatggt
     4741 aattgttgct gatggaacag aggaagcaac aaaaagatta agtagagttt taagaaatga
     4801 tccaggaatg ggagtaatta gacatgcaga tgctggttac gaattagcaa aacaaactgc
     4861 tcaagaacat gggttggatt tagttgcaaa acaaaacagc taacctactt aatgccttac
     4921 tataatacag aagaaagcat ccaacatgta gtaacattat taaacaaacg tttgtataat
     4981 gcaggatata gctttagggt tattgaagtt tcagaatata cggtttatgt acaggcaagt
     5041 tttgattttg gatattatgt aaatgtgcat atacaatttg ataactgcat ttttactaat
     5101 ttaaaagaaa gagatgagtg gccagatgct tggcatgcag atcagctttt tcttttggat
     5161 gaagtggaag tggaaaatat attgaactgg tatgatgtag aaaaggagcc tacaaacaat
     5221 ttatttgctt ttatttttaa tataaatgga tgtaatatta acaatgctgg cgtagttgtt
     5281 tgcgaaaaat tatatattcg ttgggaacat ccttattatg actgataata gaaaaataaa
     5341 tagcttagtt agatattgaa gttttaattt acatccttat ttttgcaaac gttaaatacc
     5401 aattatggaa caacaacaca aactagctga agagcaaaat aaacaagact ttacaaagag
     5461 ttgggttagt tcatctcgtt ttttatttta tttattaatc acttgtatga tggcctctac
     5521 aataggtggc tgttttaaac tatataaaag taaatataag ggtacaccag agtatgaaat
     5581 acaaggcagt actaaataca ctccagatta taaataaatt ttaaaaaaaa ttttgtttag
     5641 aactttggtt atatattttt gcacccccaa ttagttcttt aaacatactg cggaagtagc
     5701 tcatttggta gagcacaacc ttgccaaggt tggggtggcc ggttcgagcc cggtcttccg
     5761 ctctaaattt tgatttttcg aaattttaag gttgccctgg tggtggaatt ggtagacacg
     5821 caggacttaa aatcctgtgg ccagcaatgg ccgtaacggt tcaagtccgt tccggggcac
     5881 taaaaggttg tttcattttt atgattcaac ctttttttat ttataagcta atctttccag
     5941 agaaagggaa ataatataat tgctaataaa acaaccatta catctaatgc tatcaacata
     6001 agtattaact gcccccaact ttcttgtata acatttgaaa aagctggtgt tgttattttc
     6061 attaataaaa gtaattgtgg aataattaat ggaaaaccta agatagccat taatgcagcc
     6121 tgctgcattg ctttagcagc tattgctgat aaaaaagtaa atactaaact taaacttgta
     6181 cctcctagta ttgtaatgcc tacaaaaagc attgcattat gcattgggtt atttaataaa
     6241 accataaaaa gcaataagct aattaggctc attaaaagca ttagtaaaag gttaaaaatt
     6301 aatttactta aaataaaaac cttagcaccg gcaatggtat aatagtatat cattttaccc
     6361 ttactttccg ataaaaaact ttttgctaca gcatttgtaa caataaataa ttgtacaatc
     6421 caaaataacc cattccatat ttgttcttcg ggctggccca tagttaaata aataacaaat
     6481 atggtactag ccacatacaa aataattccg taaaaagagt attgttgcct tacttctagc
     6541 aaaatatctt tttttatgag actaataatt tgtttcaatt caggcattaa aaaatgagtt
     6601 ttttatttat gataacaaac cctgcatctt ggctcatata catctttttc tcctaataaa
     6661 acctgcccat cttgttctat ttttctgtag gaaatattag caatattacc acattgcaca
     6721 catattgcat gcaacttagt aatataatct gctatggcta aaaggttagg catttggcca
     6781 aatggtttgc caagataatc catatctaaa cctgcaacaa ttacacgtat tccttttgtg
     6841 gcaagtgttt cgcaaacatt cgttatttca tcatcaaaaa attgtgcttc atcaattcct
     6901 actacatcta catcttgcgt taacaataaa attttttgag aattatctac aggtgtactt
     6961 tgtattgcat tagcatcatg gcttacaact tttgtttcat catatcttac atctattcct
     7021 ggtttaaaaa tttctacttt taagttagca attttggcac gttttaatct tctaatgagt
     7081 tcttctgttt ttccactaaa catactgcca catatcactt ctatccatcc tcttctttct
     7141 ccacttaaat taggttctat aaacatttta tttaacttgg gtttattagt aaaagcgtat
     7201 aactttattg cctaaatatt actatttaga aacctcaaat gtatactatc caatggaaag
     7261 agttggcaca cttatcaaca aattaaaaga gcaactagac caaaagcaag atgcagctaa
     7321 attattgctt actgcacaaa tgcttttagc cgaattgcag caacaacagc ctagttcaaa
     7381 tagtggaaaa gtatctgtta caatgcctag tgttactttt gttagtgaaa ttattgcaga
     7441 ggtgaaaaat gaaagtaaac caaaagctgt agaacaacct aagaaggctc cacaaaatga
     7501 aaaccaaaca gggtggttgt ttaatcctga attagcttct atacctacac ttgttcatca
     7561 agaaattgta acaaatgaag tagttgataa agtggaggat aaaaaagaat tgtttgaatt
     7621 aaaagataca cttgtaacag aggaggagcc aagccttaat gatattttaa aagaggaaaa
     7681 aaccgaaata gcaaccgtat taaaaggagc acccattaaa gacttaaaaa aagctattgg
     7741 tattaacgac agatatgttt ttatcaacga gctttttagg ggagatgaag ttatgtacga
     7801 acgtagtatt aaaaccatta atacttttaa tatttatgcc gaggcagaat attggataca
     7861 aagagaatta aaggtgaagc ttggatggga agaaaataat tctacagtag aaagttttaa
     7921 tcaattagtt aaaaggcgtt ttgcataaat aaaatctaca ataatttttg ttgccccact
     7981 attattttga acataatttt ctgcattaat tgcagtagtt tcataaaaca aattatctgt
     8041 taatagctta ttaaatactt gctcaagttc taaagcattt tctatactta ttccaccatt
     8101 gttttctact aattcttttg cttctatata tttttcaaat tctggcccaa atactacagg
     8161 ttttttatat actgctgctt ctaaaacatt gtgtacacca tcgtctccaa atgcaccacc
     8221 tacaaagcat attgttgcat atttgtataa cttgcttaac atgccaatat tatcaattat
     8281 taaaacattt ttttctgtaa aattgctatt tggctcgtta actaatacag aaaaaagtat
     8341 agctttttta tacaacttta aacattcatt aaccctttca gtttctattt ggtgcggtgc
     8401 aataataaat tttatgtgag tattagtgtt ggcataatgg tctaaagctt catcatcatc
     8461 agtccaggtg ctacctgcaa caataacctt atcagttcca ataaattttt caataatttc
     8521 aatactttca aaactgtttg ctatttcaat aactctatca aatctagtat cgccagcaat
     8581 agaaacattt tttgtaaagc ctattttatg tagtaaatca gcacaattat tatcttgaac
     8641 aaaaaagtaa gaaaagcact gcagcatttc tctatatatt tttccgtacc acttaaagaa
     8701 aggttgattt tttctaaaac ttgcggataa tagtagcaag ggtatatttc ttttcttaat
     8761 ttctgttaaa taataatacc aaaactcata tttgatgaat aaagtaagtt taggttgtac
     8821 aatatctaaa aattgggtag cattcttagc cgaatctaat ggcaagtaat aaacccaatt
     8881 agcataagca taattctttt gaatttcata acccgatgga gaaaaaaaag ttaataaaat
     8941 tgtataggat ggatacgttt gttttatttt ttctaaaacc ggccttcctt gctcaaactc
     9001 acctaagcta gagcaatgca tccaaattac ttcgctttta ttattggcaa atgcttcttt
     9061 tagtttagta aaaatatttt ttctaccagt aagccacaac ttggcttttg tatttttggt
     9121 acttataatt tttgcaatga aaggataaac tgcaataaat atgttgtaaa aaattttcaa
     9181 tagattgttt gttggtacaa agaaaataaa atagcttaaa ataaactatt aagttaaaga
     9241 gttttgctaa caaattaaac gtattatttt ttctaatacc tttgcccaca tttattttta
     9301 actatgaagc ctatacaaat ggtagatact aaaacacagt atctaaaaat taaagaagag
     9361 gtagataaag gaatttttga agtattagaa agtgcacaat atattggagg tgcaaaagtg
     9421 aatgaatttt ctgaagcatt aaatgtttac cataatagtg cttttacaat tacttgtgct
     9481 aatggtacag atgctttaca aatagcatta atggcattag acttacagcc tggcgatgaa
     9541 gtaattacac cttcttttac ttatattgct actaccgagg tggttgcttt actgcgttta
     9601 aaacctgttt ttgtagaagt aaatccaaaa actttttgca tagaaccaga tgctataaga
     9661 aaagcaatta cacctaaaac aaaggccatt gttcctgtac atttatatgg gcatgcagca
     9721 ccaatggaag aaattatgca aattgcaaaa gaatataatt tgtatgtagt agaagataat
     9781 gctcaggcta ttggttgtaa ttatactttt aataatggca ctacaaaaaa aactgctaca
     9841 attggccata ttggttgtac atctttttat ccaagtaaaa acttaggagc ttatggcgat
     9901 ggtggtgcaa tttttacaca agatgcaatg cttgcagaaa aattaaaaat gattgccaac
     9961 catggtcaaa aaacaagata cattcatgat attgttggtt gcaactcaag gttagatgct
    10021 atacaagctg caatattaac cgttaaatta aaacagttag ataattatat tgttgcccga
    10081 aataatgctg ctaattatta caataaagca tttgcaaatc atacaaaaat tactactcct
    10141 tttgttgcct cttacagtaa ccatgtgtat catcaatata ctttaatact tgatggagta
    10201 aatcgagatg gcttagtaaa ttacttggca gagaaaaaaa ttccatctat gatttattat
    10261 cctattcctg gtcataaaca aaaagttttt gaaaccattg gtagcgaaaa atataactta
    10321 gaaattacag attggttaac agaaaaagta attagtttac caattcatac agagcttgaa
    10381 acagatcaat tagaatatat aactacagaa gttttaaatt atataaacaa ccactaaata
    10441 aaatatgaaa atagcagttg taggcacagg atatgtaggc ttagtatcag gaacatgttt
    10501 tgcagaaaca ggtaatacag ttacttgtat tgatattgac caaaataaag tatctaaact
    10561 tagtaatggc gaaataacca tttatgaacc agggttagaa aaaatatttc taagaaattt
    10621 aaaagaaaat cgtttaaaat ttactaccaa tttagctgag ggtattaaag atgcagccat
    10681 tgttttttta gcattaccaa caccacctgg cgaagatgga agtgcagatt taaagtatgt
    10741 gttgggagtg gccaatgact taggaaaaat tataacagac tataaagtaa ttgttgataa
    10801 aagtactgta ccagtaggta cagcagcaaa agtacatgct gcaattgctg ccaatgcaaa
    10861 agttgaattt gatgtggtaa gtaatcctga gtttttaaga gaaggtgttg ctgtagatga
    10921 ttttatgaaa ccagatagag tggtaattgg aactaacagc gatagagcca aaaaagtaat
    10981 ggcagattta tatgcacctt atgtacgaca aggaaatccg ataattttta tggatgaaaa
    11041 aagtgctgaa ctaacaaagt atgcagccaa ttctttttta gccactaaaa tttcatttat
    11101 gaatgagata gcccagcttt gtgaaaagtt aggagcagat gtggatatgg ttagaaaagg
    11161 aattggtagc gatgatagaa ttggaaaaag atttttattt ccaggtattg gatatggtgg
    11221 aagttgcttt cctaaagatg tgcaggcttt aataaaatca tcggacgagg tgaattataa
    11281 ttttcaaata ttaaaggctg tagaagaagt aaacgaagca caaaagctac atcttattcc
    11341 aaaaataaaa aaatatttca ataacaattt acaaggaaaa cattttgcat tatggggttt
    11401 ggcatttaaa ccaaatacag atgatataag agaagcacca gctttatatg tgatagatgc
    11461 attagtaaat gcaggagcaa cagttactgc ttatgaccct gaagcaataa aaaatgtaag
    11521 caatctaatt ggcaataaaa ttgcgtatgc agataaccaa tatgctgctt tgcaaaatgc
    11581 agatgcctta attattgcta cagaatggag tgaatttaga actccagatt ttgacagagt
    11641 tgctggtaca cttaaaaata aaattatttt tgatggacga aatttatttg acgtacaaca
    11701 aataagagaa atgggttatt attatgaaag tattggcaga ccataataaa gtttaattta
    11761 tttaaattat aataatgaga aaaaaagttt taataacagg tgccgcaggt tttttaggta
    11821 gccatttatg cgatagattt attaaagaag actatcatgt aattgctatg gataatttaa
    11881 ttaccggcga cctgaaaaat attgaacact tatttccgtt agaaaatttt gaattttata
    11941 accacgatgt taccaagtac attcatatat ccggaaactt agattatatt cttcactttg
    12001 catctcctgc aagccctata gattatttaa aaattcctat tcaaacatta aaagtgggtg
    12061 ctttaggaac tcataattgt ttaggattag caaaagagaa aaaagccaga attttagtag
    12121 ccagcacaag tgaagtgtat ggagatcctt tagtacatcc acaaacagaa gaatattggg
    12181 gcaatgtaaa ccctgtggga cctcgtggtg tttatgatga agcaaaaaga tacatggaaa
    12241 gcattacaat ggcttaccat acttttcata atgtagaaac aagaattgta agaatattta
    12301 atacttatgg gccacgtatg aggctgaatg atggaagagc cttaccagct tttgttggcc
    12361 a
//
LOCUS       DAOQPK010000046        11775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_2804_length_11775_cov_4.005205, whole genome shotgun sequence.
ACCESSION   DAOQPK010000046 DAOQPK010000000
VERSION     DAOQPK010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11775
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2804_length_11775_cov_4.005205"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="PLU36_03725"
     CDS             complement(<1..1153)
                     /locus_tag="PLU36_03725"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMZ45889.1"
                     /translation="MRVIIVGGGFAGLKLARLLNNKKGIDITLLDKYNFHQFQPLFYQ
                     VATAGLDASNISFPLRKVFQKSKNIRIRLAEVQKIDSKENKVVTDIGIFEYDYLVLAT
                     GANTNFFGNELLQKHAFPMKSTVEALQIRHRLIQNLEDALLLQNKHEIEKLLSIVVVG
                     GGPTGVEVSGSLAEMKKYVLPKDYPEIDFSLMKIYLLEGTDKTLASMSNKSSMHSLQY
                     LQKLGVDVYLNSILESYDGQKVRLKNGIEIPTNTVIWAAGIKGNVPNGIENTIITRGN
                     RIKVDRQNKVLHHQNIFALGDVAYMETTLYPNGHPQVANVAINQAKNLAKNILLLQKN
                     NSNLLEFEYKDKGSMATVGRNLAVVDFQKPSLHFKGFIAWYIWMGLHLFL"
     gene            complement(1225..2724)
                     /locus_tag="PLU36_03730"
     CDS             complement(1225..2724)
                     /locus_tag="PLU36_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HMZ45890.1"
                     /translation="MSGIKKLAGQTMWYGLSTIAAKFINYILTPYITFKFTNAQYGEM
                     SIIYSFIPFMNIIFTYGMETAYFRYANTYDAKKIYNTISISIIISSIIFSVIMLLMAK
                     PLTTLLTIEADNNYLVLAVCIIFLDALSTLPFAKLRQENRPLKFAFIRITSILINVFC
                     IYFFLSLCPNWLKENPTHWCSKFYQPNFAVGYILIANIVQSLFTLLLLSKEFFSFSLN
                     FDTKLWKEMMLYSLPLIIAGFGGMINETFDRIMLSWWAPVQTEMAAKSEVGIYAACYK
                     LSILITLSVQAFRMGAEPFFFKKSKDADAKKTYARVMNFFVITVCFMFLLVALYLDIW
                     KHFIQNANMWQGLHIVPILLLANIFLGIYYNLSIWYKLVNKTNVGALITIFGVVITLV
                     INYFFIPYYSYTACAWATFACYGSMMVLSYLWGQKEYPVPYEWVKLCSYIFVAVALYF
                     IHTILIKHFTTFWFSVTIASLLVLLFSFIVFIKEKSELKNLPIFNKLIK"
     gene            2820..3386
                     /locus_tag="PLU36_03735"
     CDS             2820..3386
                     /locus_tag="PLU36_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45891.1"
                     /translation="MKKFFYLLVLMLIIKTTNAQETSIIVGGETKPAISRDVNEKESD
                     VIDALKYYFQQKGVKKFKKSKGIYTVKGIKINEITNDNLDLFFSIDRKGKKKDKLTTV
                     SMAVHTTERQFVGESTHEQIKQKLPAFMDLLPTIVSDYHKKLEADRLQKEADDARKEK
                     EKLEKQAAKQAAKAAKLQKEADQAKTEK"
     gene            3388..3951
                     /gene="ruvC"
                     /locus_tag="PLU36_03740"
     CDS             3388..3951
                     /gene="ruvC"
                     /locus_tag="PLU36_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HMZ45892.1"
                     /translation="MTTKPHIILGIDPGTQLLGFALLKVEGNKQQVLLMDVLKLTSHK
                     DIYVRLEKIHSKVTELIKLYQPKTFAIEAPFFGKNVQSMLKLGRAQGVAIAAAIHAGL
                     DVTEYSPKKVKQSITGNGNADKEQIWKMLQRVLNIDEKPQYYDATDALAVALCHCYQL
                     SSSLNLKDKKFNGWNDFIQKNPNRFKQ"
     gene            complement(4141..5412)
                     /gene="kynU"
                     /locus_tag="PLU36_03745"
     CDS             complement(4141..5412)
                     /gene="kynU"
                     /locus_tag="PLU36_03745"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HMZ45893.1"
                     /translation="MIYQNTLAFAKNLDEQDEIKDFRTKFFIPQHNGRDTVYLTGNSL
                     GLQPKSTAAYIQQELNDWANLGVEGHFHAKNPWMPYHEIFPKQLSKIVGALEEEVVVM
                     NQLTVNLHLLMVSFYQPTAQRYKIICEAKAFPSDQYAIESQVRFHGLNYEDAVIEVEP
                     REGEHTIREEDILNTIEQNKDSVALVLFGGVNYYTGQVFNIKNITEASHKVGALVGFD
                     FAHATGNINLQLHNWNVDFACWCSYKYLNSGPGGVAGAFIHQKHITNKKLKRFAGWWG
                     YNKETRFKMERGFIPIPTAEGWQLSNAPVLSMAAHKAALDIFDEVGMDRLHKKRKLLS
                     SFVLFILDDINQKSNTQTIEIITPRNENEIGCQVSMLMLKNGKQIFNALMQQGVIADW
                     REPNVIRIAPVPLYNSFEDVWKFGDIIKQIV"
     gene            complement(5420..5839)
                     /locus_tag="PLU36_03750"
     CDS             complement(5420..5839)
                     /locus_tag="PLU36_03750"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HMZ45894.1"
                     /translation="MENKVIHTELAAKPLGSYPHAVKVGNLLFLSGIGSRKPEDNSIP
                     GLQLDSCNNIIQYDIEAEVHQVMANVKAVLEASGSCWKNIVDVTVFLTNMKKDFPIYN
                     KLYGEYFKDVQACRTTVEVKSLPTPIAIELKVIATIN"
     gene            complement(5909..7366)
                     /locus_tag="PLU36_03755"
     CDS             complement(5909..7366)
                     /locus_tag="PLU36_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HMZ45895.1"
                     /translation="MNLEIPYYIENYIGGNLVAPLSSQFIDCVNPATAEVFAQIPNSN
                     EKDIELAVQAATIAKESWGNTTLEERFKILNRIADLIDENIDAFALAETNDNGKPLWL
                     SKKVDIPRASANFRFFATGIMHFASESHSMQGKAINYTLRQPIGIVGCISPWNLPLYL
                     FTWKIAPALAAGNCVIAKPSEVTPITAFLLSKICIIAGLPAGVLNIVHGLGNSCGEAI
                     VKHKKIKAISFTGSTRAGEQIASIAAPMFKKLSLELGGKNPNIIFADCNWDKMMKTTI
                     QSSFSNQGQICLCGSRILIEESIYQKFKSEFIEKVKSLTVGDPLHENSKQGAVVSKSH
                     FDKVLNCIEIAKNEGGKILLGGNAVKLEGRCANGYFIEPTIIEGLDANCTTNMEEIFG
                     PIVTLQTFKTLEEALQLANTSNYGLSATIWTQDISKANYIAAKTESGILWVNCWLVRD
                     LRTPFGGNKNSGIGREGGWEALKFFTETKNVCIEF"
     gene            complement(7427..8212)
                     /locus_tag="PLU36_03760"
     CDS             complement(7427..8212)
                     /locus_tag="PLU36_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ45896.1"
                     /translation="MNISLENKTAIICGSTQGIGLATAKELSNLGATCILMARNQTAL
                     IEALKQLNYSFNQPHNYLVADFDNPSTVKSAMVEFIKKNTVHILVNNSGGPNAGQITD
                     ATEQQFLDTFNRHLICNHILTKAVINGMKEAGFGRIINIISTSVKIPLPNLGVSNTIR
                     GAVASWSKTMANELGQFNITVNNVLPGATATGRLKNIIENNASKKNITLEEIENEMQK
                     EIPMKRFAQPEEIANAVAFLASPAASYINGVSIPVDGGRTGSL"
     gene            8327..8848
                     /locus_tag="PLU36_03765"
     CDS             8327..8848
                     /locus_tag="PLU36_03765"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF009763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HMZ45897.1"
                     /translation="MPVIPPFNLKKWIDENRDLLKPPVGNSCVYKDAENFIVMIVGGP
                     NARKDYHFNETEELFYQIEGDITVKIIDEGVFKNIHIKEGEMFLLPPKTPHSPQRGPN
                     TVGLVIEKKRESEDDGFLWFCENCGNKLYEETLHVDDIVKQLPPVMNRYWESIMHRTC
                     DKCGSVMEPPVKK"
     gene            8926..9912
                     /locus_tag="PLU36_03770"
     CDS             8926..9912
                     /locus_tag="PLU36_03770"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMZ45898.1"
                     /translation="MKIDIHTHIMPAQMPNWVKKFGYGEFIHLEHRNCKACMMKGDKL
                     FRVVEDNCFETDLRLNEMDATNVTIQVLSTIPVLFNYWAKPKDGYETSRFLNDHIAES
                     VQKHPNRFIGIGTVPLQDIDFAIKEMERCVTKLNMPGVEIGSNINGKNLSDSYFFPFY
                     EAAEKLSCALFIHPWEMMGEKDMQKYWLPWLVGMPAETSRAICSLIFGGVFEKFPKLR
                     VAFAHGGGSFPFTLGRIEHGFNVRPDLVAIDNPINPCNYIGKFWIDSLVHNEDALKYN
                     LKLIGEDKICLGSDYPFPLGEHHPGKLIEESSLNNTVKEKLLYKNAYNWLGK"
     gene            9978..10736
                     /locus_tag="PLU36_03775"
     CDS             9978..10736
                     /locus_tag="PLU36_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HMZ45899.1"
                     /translation="MSYAGKNLRYLRKLKNWTQEEFANRLQIKRSLLGAYEEERAEPK
                     LEVLELVSGIFKLSLEELLLKELTNDNSATSYLEQRRKLKNLQSSNGLIHFVPFKAAA
                     GYLNGYADANFIDELNTFTLPMLTSGEYRAFEIIGDSMMPTPSGSVIVGEKVEQLDDV
                     KNSNTYVLLSKSDGIVYKRLIKNTKPKNTLTLVSDNPVYEPYQMQAQDVLEVWKAVYI
                     LQKASTVQRWEVGQLAGMVNDLQTQVTNLNKKIN"
     gene            10900..>11775
                     /locus_tag="PLU36_03780"
     CDS             10900..>11775
                     /locus_tag="PLU36_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HMZ45900.1"
                     /translation="MKVTVVGAGAVGATCADNIARKELATELVLLDIKEGLAEGKAQD
                     MMQTAALLGFDTVITGSTNDYTKTAGSNVVVITSGIPRKPGMTREELIGTNAGIVKGV
                     CENILKHSPNAIIIIISNPMDTMTYLALKSTGLPKNRIIGMGGILDSSRFKYQLSQHL
                     GANPNDLNAVVVGGHGDTTMIPLISKATWNSAPVTDFLSDEQQQKVVNDTMVGGATLT
                     KLIGTSAWYAPGAAGAALVEAIVRDEKKLFTCCVALDGEYNQNDICLGVPVIIGKNGW
                     EKIIDFKLSSSEQDAF"
BASE COUNT         4196 a         1793 c         1826 g         3960 t
ORIGIN      
        1 ataaaaacaa atgtaacccc atccaaatat accaagcaat aaatccttta aaatgtaaag
       61 agggtttttg aaaatcaaca acagctaagt tccttcctac agtagccata ctacctttat
      121 ctttatattc aaattctagt aaattagaat tatttttttg aagtaataaa atattttttg
      181 ctaaattttt agcttggtta atggcaacat ttgctacttg aggatgacca ttaggataca
      241 gagtagtttc catataagca acatctccta aagcaaaaat attttgatga tgtagaactt
      301 tgttttgtct gtcaactttt attctattgc ctcttgttat aatggtattt tctattccat
      361 taggtacatt accttttata ccagcagccc aaataactgt attggtaggt atttcaatac
      421 catttttaag tcttactttt tgcccatcgt agctttctaa tatggagttt aaatacacat
      481 caacacctaa tttttgtaga tactgtaagg agtgcatact gcttttattg ctcatagatg
      541 ctagtgtttt atctgtgcct tccagcaaat aaattttcat taaggagaaa tctatttctg
      601 gataatcttt aggtaaaaca tactttttca tctctgctaa agagccacta acttcaactc
      661 ctgttgggcc tccaccaacc acaacaatag ataataattt ttcaatttca tgtttatttt
      721 gcaacaacaa tgcatcttct aaattctgta taagcctatg ccttatttgc aaagcctcca
      781 cagtgctttt cattgggaaa gcatgcttct gtaaaagttc atttccaaaa aagttggtat
      841 ttgcaccagt tgcaagtact aaataatcat actcaaaaat gccaatatct gttactactt
      901 tattttcttt agagtcaatt ttttgtactt cagctaacct tattctaata ttcttacttt
      961 tttgaaatac ttttcttaag ggaaaggaaa tgttgcttgc atctaatcca gcagttgcaa
     1021 cttgataaaa taatggctga aactgatgaa aattatattt atcaagtaaa gttatatcta
     1081 tacccttttt attattcaat aatcttgcaa gttttaagcc tgcaaatcct ccaccaacaa
     1141 taattactct cattttaatt gcattattta aagcaaatat aggaaattat ttaatatggt
     1201 taggattact aactatatta acaattactt aattagttta ttaaaaatgg gtaaattttt
     1261 aagctccgat ttttctttga taaaaacaat aaaggaaaat agtaaaacta ataagctagc
     1321 aatagtaacg ctaaaccaaa acgtggtaaa atgtttaatt aaaatggtat gaataaaata
     1381 caaagcaacg gcgacaaaaa tataagaaca taattttacc cattcataag gtacagggta
     1441 ttctttttgt ccccatagat aacttaaaac catcatacta ccataacaag caaaagttgc
     1501 ccaagcacag gctgtatagc tataataagg aataaaaaag tagtttatta ccaaggttat
     1561 cactactcca aaaatggtta taagggcacc aacatttgtt ttatttacta gtttatacca
     1621 aatacttaaa ttataataaa tacctaaaaa aatgtttgcc aataataata taggaacaat
     1681 atgtaagcct tgccacatat tagcattttg aataaaatgc ttccaaatat ctaaatataa
     1741 ggcaaccaaa agaaacataa agcaaacagt aattacaaaa aaattcatta ccctagcata
     1801 ggtttttttg gcatctgcat ctttagattt tttaaagaaa aatggttcgg cacccattct
     1861 aaatgcttga acagataaag tgatgagtat ggataattta taacatgctg cataaatacc
     1921 cacttcagat tttgcagcca tttctgtttg tacaggagcc caccagctta gcattattct
     1981 atcaaaagtt tcatttatca ttcccccaaa gcctgctatg attaatggta aactatacag
     2041 catcatttct ttccaaagct tggtatcaaa gtttaaacta aaactaaaaa actctttaga
     2101 taataataat aatgtaaaaa gagactggac aatgtttgca attaaaatat acccaactgc
     2161 aaaatttggt tggtaaaact tactacacca atgagtagga ttttctttta accagtttgg
     2221 gcaaaggctc aaaaaaaagt agatacaaaa tacattaatt aatatactgg taattctaat
     2281 aaatgcaaac tttagtggcc tgttttcttg ccttagttta gcaaatggta atgtacttaa
     2341 ggcatctaaa aaaataatac aaacggctaa aactaaataa ttattatcgg cttcaattgt
     2401 taataatgtg gttaatggtt ttgccattaa tagcattatt acactgaaaa taatagaaga
     2461 aataataatt gaaattgaaa tagtattgta aatcttttta gcatcatagg tattggcata
     2521 cctaaaataa gctgtttcca tgccgtaagt aaaaataata ttcataaaag gaatgaaaga
     2581 atatataata ctcatttcac catactgtgc gttggtaaac ttaaaggtaa tatacggtgt
     2641 aagtatgtaa ttaataaact tggcagctat ggtacttaat ccgtaccaca ttgtttgacc
     2701 tgcaagtttt tttattccac tcaatagtta aaatttatgt atcaaaaata gtaatgatta
     2761 tggtattaca aattatgtta ttaattttga aataattatt aaactaaatt taagaatcaa
     2821 tgaaaaagtt tttttacttg ttagtgttaa tgctaattat aaaaacaacc aatgcacagg
     2881 aaacttccat aattgtgggt ggtgaaacaa agccagcaat tagtagagat gtaaatgaaa
     2941 aagaatcgga tgtaattgat gcattaaagt attattttca acaaaaagga gttaaaaaat
     3001 ttaaaaaatc aaaaggcatt tatacagtta aaggaataaa aattaatgag attaccaatg
     3061 acaacctaga tttatttttt agtattgata ggaaaggtaa aaagaaagat aaattaacta
     3121 cagtttcaat ggcagtacat actacagaaa ggcaatttgt aggtgaaagt acacatgaac
     3181 aaattaaaca aaaattacct gcttttatgg atttgttacc aacaattgta tcagattatc
     3241 ataaaaaatt agaagcagat agattgcaaa aagaagcaga tgacgcaaga aaagaaaaag
     3301 aaaaattaga aaagcaagct gccaaacaag cagcaaaagc agcaaaattg caaaaggaag
     3361 cagaccaagc aaaaactgaa aaataatttg acaacaaagc cacatattat tcttggtata
     3421 gaccctggta cacagttact agggtttgct ttattaaaag tagaaggaaa taaacaacaa
     3481 gttttattaa tggatgtttt aaaactaact tctcataaag atatttatgt aaggttagaa
     3541 aaaattcatt ctaaggttac agaattaatc aagttatatc aaccaaaaac ttttgccata
     3601 gaagcccctt tttttggtaa gaacgtacaa agtatgttaa aacttggtag ggcacaaggc
     3661 gttgcaatag ctgctgcaat tcatgctggt ttagatgtta cagaatattc tcctaaaaaa
     3721 gtaaaacaat ctattacagg taatggcaat gcagataaag aacaaatttg gaaaatgttg
     3781 caacgagttt tgaatattga tgaaaagcct caatattatg atgctacaga tgctttagca
     3841 gttgctcttt gtcattgtta tcaactatca agtagtttaa atttgaagga taaaaagttt
     3901 aatggttgga atgattttat tcaaaaaaat ccaaatagat ttaagcaatg ataaaccatg
     3961 tatcgttaaa aaatattaat tcacctatta atatatacaa tttaccgaag tatagcccca
     4021 atagcccatg tacatttaaa gattggtata attttttgta atattacatc agttttttaa
     4081 gggtttaggg ttgaaaagga ggctacgatt cttcgctgcc tcctttaatt ttttacagaa
     4141 ttatacaatt tgctttatta tatctccaaa tttccaaaca tcttcaaaag agttgtaaag
     4201 tggtacaggt gcaattctaa ttacattagg ttctctccaa tctgcaatta ctccttgctg
     4261 cattaaagca ttaaatattt gttttccgtt ttttagcatt agcatagaga cttggcaacc
     4321 tatttcattc tcatttctag gcgtaataat ttcaatggtt tgggtattgc ttttttggtt
     4381 gatatcgtct aaaataaata aaacaaaact gcttaatagt tttctttttt tatgtaacct
     4441 atccatacca acttcgtcaa aaatatctaa ggctgcttta tgagcagcca tgcttaaaac
     4501 aggtgcatta cttaattgcc atccttcggc agttggtatt ggtataaaac cacgttccat
     4561 tttaaaacgt gtttctttat tatatcccca ccaaccggca aatcttttta attttttatt
     4621 tgttatatgt ttttgatgta taaaagctcc tgcaacacct ccagggcctg agtttaaata
     4681 tttataagaa caccaacacg caaaatctac attccaatta tgcagttgta agttaatatt
     4741 tcctgtagca tgggcaaaat caaatccaac caatgcacct actttatggc ttgcctcagt
     4801 aatattttta atattaaaaa cttgtccggt ataataattt actcctccaa ataatactaa
     4861 agccacactg tctttatttt gctcgattgt gtttaaaata tcttcttctc ttatagtgtg
     4921 ttctccttct cttggttcta cttcaataac tgcatcttcg taattaagcc catgaaacct
     4981 tacttggctt tcaatagcat attgatcgga tggaaaagct tttgcttcgc aaattatttt
     5041 atatcgctgg gctgttggtt gataaaaact taccattaat aaatgaagat taactgtaag
     5101 ttggttcatt accacaactt cttcttctaa tgcacctaca atttttgata attgtttagg
     5161 aaaaatttca tggtaaggca tccatggatt tttggcatga aaatgtcctt caactcctaa
     5221 attagcccaa tcatttaatt cttgttgaat ataagctgct gttgattttg gttgaagacc
     5281 taatgaattg cctgttagat aaacagtatc tctaccatta tgctgtggaa taaaaaattt
     5341 agttctaaaa tcttttattt catcttgctc atctaaattt tttgcaaatg ctaatgtatt
     5401 ttgataaatc attttaaagt tagtttattg tagcaataac ttttaattct attgctattg
     5461 gtgttggtaa actttttact tctactgtgg tacgacaagc ttgaacatct ttaaaatatt
     5521 cgccataaag tttattataa ataggaaaat cttttttcat attggttaaa aaaacagtaa
     5581 catctacaat attcttccaa caactaccac tagcttctaa aactgctttt acatttgcca
     5641 ttacttgatg cacttcagct tcaatatcat attgaataat gttattgcag ctatctaact
     5701 gcaaacctgg tattgaatta tcttctggtt ttcttgagcc aattccagaa aggaataaaa
     5761 ggttgccaac ctttactgca tgaggataag agcctaaggg ttttgcagct aattccgtat
     5821 gaataacttt gttctccatt ttatttttaa attacaagta ttgaaaataa agtatgcttt
     5881 aattgaaata aactaagaaa aaagagtatt aaaattcaat acaaacattt ttagtttctg
     5941 taaaaaactt taaagcttcc caaccgcctt ctcttcctat tccactattt ttattaccac
     6001 caaaaggtgt tcttaaatct ctaactaacc aacaattaac ccataaaatt ccactctctg
     6061 tttttgcagc aatataatta gctttagaaa tatcttgagt ccaaatagtt gcagataaac
     6121 catagttact tgtgttggct aattgcagag cctcttccag agttttaaaa gtttgtaaag
     6181 taactatagg gccaaaaatt tcttccatat ttgttgtaca attggcatct aacccttcaa
     6241 taattgttgg ttcaataaaa tatccattgg cacatcttcc ttctagtttt actgcattac
     6301 ctcctaataa aattttgcca ccttcatttt ttgcaatttc aatacaattc aaaaccttat
     6361 caaaatgaga tttgctaaca acagcacctt gttttgaatt ttcatgtaaa ggatctccaa
     6421 ctgtaaggct ctttactttt tcaataaatt ccgatttgaa tttttgatag atgctttctt
     6481 caattaaaat tctgctccca cataaacata tttgtccctg gttactaaaa gaggattgta
     6541 ttgttgtttt catcatttta tcccaattac aatcggcaaa aatgatatta ggatttttac
     6601 cacctaactc taaagaaagt tttttaaaca taggtgcagc tattgaagca atttgttctc
     6661 ctgctctggt gcttcctgta aaagaaatag ctttaatttt tttatgcttt acaatagctt
     6721 cgccacagct attgcctaaa ccatgtacaa tatttaatac gccagcaggt aaaccagcta
     6781 tgatacaaat tttagaaagt aaaaaagctg taataggggt tacttcgctt ggttttgcta
     6841 taacacaatt acctgctgca agagcaggtg caattttcca ggtaaataaa tataaaggta
     6901 aattccatgg agaaatacaa ccaacaattc caattggttg gcgtaatgta tagttaatag
     6961 cttttccttg catggaatgg ctttctgaag caaagtgcat aatgcctgta gcaaaaaaac
     7021 ggaaattagc cgatgcccta ggaatatcca cctttttact aagccataat ggtttaccat
     7081 tatcatttgt ttcggctaat gcaaaagcat caatgttttc atcaattaaa tctgcaattc
     7141 tatttaagat tttaaaacgt tcttctaaag ttgtattacc ccaactttct ttagcaattg
     7201 tagctgcttg cactgctaat tcaatatctt tttcgttgct atttggaatt tgtgcaaaaa
     7261 cttcggcagt agccggatta acgcaatcta taaactgact acttaatggt gcaaccaaat
     7321 tgccaccaat atagttttct atgtaataag gtatttcaag attcatttgt agatagagat
     7381 taaattatgg ttgcttactt aattgctttt ttagaaaaaa attcttttat aaagatccag
     7441 ttctaccgcc atctactgga atactaacac cattaatata agaagctgca ggcgatgcta
     7501 aaaatgctac agcattagct atctcttctg gttgtgcaaa acgcttcatc ggaatttctt
     7561 tttgcatttc attttcaatt tcttctaaag taatattttt tttgcttgcg ttattttcaa
     7621 ttatattttt taatctacca gttgcagtag caccaggtaa aacattattt actgttatat
     7681 taaattggcc taattcgtta gccattgttt tactccaact agctacagca ccacgtatag
     7741 tattagaaac tcctaaatta ggtaagggaa tttttacaga ggttgaaatt atattaatga
     7801 ttcttccaaa accagcttct ttcattccat taattaccgc tttggttaat atatgattac
     7861 aaataagatg acgattaaaa gtatctaaaa actgttgttc tgttgcatct gtaatttgcc
     7921 cagcattggg gcctccagaa ttattaacta aaatatgaac tgtatttttt ttaataaatt
     7981 ccaccatagc acttttcact gtagaagggt tatcaaaatc ggcaaccaaa taattgtgtg
     8041 gttggttaaa tgaatagttc aattgtttta gtgcttcaat caatgcagtt tgatttctag
     8101 ccattaaaat acaagtagct cctaaattag acaactcttt ggcagttgct aaaccaatgc
     8161 cttgtgtgct gccacaaata attgcagttt tattttcaag agaaatattc atctttgtaa
     8221 ttgataattt aattttcaaa agtagtaaaa agaaagtgta tcctttaaac gtttcttaat
     8281 aaattctttt gaagctaatt aattcataaa cttaaaaaat agtaacatgc cagtaattcc
     8341 tcccttcaat ttaaaaaaat ggattgatga aaatagagat ttattaaaac caccagttgg
     8401 caattcttgc gtatataaag atgcagaaaa ttttattgta atgattgtag gtggaccaaa
     8461 tgcaagaaag gattatcact ttaacgaaac agaagaatta ttttatcaaa tagaaggtga
     8521 tataacagta aaaattattg atgaaggggt atttaaaaat atacatatta aagaaggaga
     8581 gatgttttta ttacctccta aaacaccaca tagcccacaa agaggaccta acactgttgg
     8641 tttagtaatt gagaaaaaaa gagaatctga agatgatggt tttttatggt tttgtgaaaa
     8701 ctgtggaaat aaattgtacg aagaaacttt acatgtagat gatattgtaa aacagttacc
     8761 acctgtaatg aataggtact gggaaagtat aatgcataga acttgtgata aatgtgggtc
     8821 agtaatggaa cctcctgtaa agaagtaaat attatttttt acagaattag gtattatata
     8881 aaatagaaaa caagtaatca atacatccaa taaacttcta caaaattgaa aattgatatt
     8941 catacacata ttatgcctgc ccaaatgcct aattgggtaa agaaatttgg ttatggagag
     9001 tttatacatt tagaacatag aaattgtaaa gcctgtatga tgaaggggga taagcttttt
     9061 agagtagtgg aagataattg ctttgaaaca gatttacgat taaatgaaat ggatgccacc
     9121 aatgtaacaa tacaagtatt atctactatt cctgttttat ttaattattg ggcaaaacca
     9181 aaagatggtt atgaaacctc tcgtttttta aatgaccata ttgccgaaag cgttcaaaaa
     9241 catcccaatc gttttattgg cattggtaca gtgcctttac aagatattga ttttgcaata
     9301 aaagaaatgg aacgatgtgt aaccaaatta aatatgccag gtgtagaaat tggtagtaat
     9361 attaatggta aaaatttaag cgattcctat tttttcccat tttatgaagc tgcagaaaaa
     9421 ttaagttgtg cattatttat tcatccttgg gaaatgatgg gagagaaaga tatgcagaaa
     9481 tattggttac catggttggt tggtatgcct gcagaaacat caagagcaat atgttcttta
     9541 atatttggag gcgtttttga aaaatttcct aaattaagag ttgcttttgc acatggtggc
     9601 ggcagttttc catttacttt aggtagaata gaacatggct ttaatgtacg accagatttg
     9661 gttgctatag ataacccaat taatccatgt aattatattg gcaaattttg gatagacagc
     9721 ctggttcata atgaagatgc tttaaaatat aatttaaaat taataggaga ggataaaatt
     9781 tgtttgggta gcgattaccc atttccttta ggagaacacc atccaggtaa attaatagaa
     9841 gagtcttctt taaacaatac agttaaagaa aaactactgt ataaaaatgc ctataattgg
     9901 ttaggaaaat aattcatgaa aaataaattt ggtaattgct aaatatgtta gtattttgca
     9961 gctaaatcag ttagtatatg agttacgcag gtaaaaattt acgctattta cgcaaattaa
    10021 aaaattggac acaagaagaa tttgcaaaca gattacaaat taaaagatcc ttattaggag
    10081 cttatgaaga agaaagagca gagcctaaac ttgaagtact agagttagtt tctggtattt
    10141 ttaaattaag tttagaagag ttattactta aagaactaac caatgataat tctgccacca
    10201 gctatttaga acaaagaaga aaattaaaga atttacaaag ttccaatggg ttaatacatt
    10261 ttgtaccatt taaagcagct gcaggttact taaatggtta tgcagatgca aattttattg
    10321 atgaattaaa cacttttact ttaccaatgc taacctctgg cgaatataga gcctttgaaa
    10381 ttataggcga tagcatgatg cctacgccaa gtggcagtgt aattgttggc gaaaaagtag
    10441 aacaattaga tgatgtaaaa aacagcaata cttatgtttt gttaagcaaa agtgatggta
    10501 ttgtttataa aagattaatt aaaaacacca agccaaagaa tacgcttact ttagtgagcg
    10561 ataatcctgt ttacgaacca tatcaaatgc aagcacaaga tgttttagag gtttggaaag
    10621 cagtttatat attacaaaaa gcaagtactg tacaaagatg ggaggttggg caacttgcag
    10681 gaatggtaaa tgatttacaa acacaggtta ccaatttaaa caaaaaaata aattagtttt
    10741 tttatagggg ttagtagtaa agcctgtatc attttggtac aggttttttg tttctttata
    10801 ttcaaataac taattaaatc atttttaaga aaaaaatagt ttaacaacgt acatttgcat
    10861 ccgaaaattt attcttccat aataaaaaat atataaagta tgaaagtaac agtagtaggt
    10921 gcaggagctg taggagcaac ttgtgccgat aacattgcac gtaaagaact tgccacagaa
    10981 ttagttttat tagacattaa agaaggttta gcagagggaa aagcacaaga tatgatgcaa
    11041 actgctgcat tacttgggtt tgatactgta attacaggta gtacaaacga ttatactaaa
    11101 actgcaggca gtaatgtagt ggtaataact tctggtattc ctcgtaaacc aggaatgaca
    11161 agggaagaat taattggtac caatgcaggt attgtgaaag gagtttgtga aaatatttta
    11221 aaacatagcc ctaatgccat tataattatt attagcaacc caatggatac catgacttat
    11281 ttagcattaa aatctaccgg gttacctaaa aatagaatta ttggtatggg tggtatttta
    11341 gatagtagcc gttttaaata tcaattaagc caacacttag gagcaaaccc taacgactta
    11401 aatgctgtgg tggtaggagg acatggagat acaactatga ttccacttat tagtaaagca
    11461 acatggaata gtgctccagt aacagatttt ttgtctgatg aacaacaaca aaaagttgta
    11521 aatgatacaa tggtaggcgg tgccacttta actaaactaa ttggtacaag tgcatggtat
    11581 gcaccaggtg ctgcaggtgc agctttagta gaagcaattg ttcgtgatga gaaaaaacta
    11641 tttacttgtt gcgtagcttt agatggtgaa tacaaccaaa atgatatttg cttaggagta
    11701 ccagttatta ttggtaaaaa tggatgggaa aaaattattg attttaaact aagtagctct
    11761 gagcaagatg cattt
//
LOCUS       DAOQPK010000047        11694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_2837_length_11694_cov_4.328808, whole genome shotgun sequence.
ACCESSION   DAOQPK010000047 DAOQPK010000000
VERSION     DAOQPK010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11694
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2837_length_11694_cov_4.328808"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..1548)
                     /locus_tag="PLU36_03785"
     CDS             complement(166..1548)
                     /locus_tag="PLU36_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HMZ45901.1"
                     /translation="MSKKMRTLTEPFKIKMVEPLRITTEEYRQNALEKAGYNPFLLRS
                     DDVFIDLLTDSGTGAMSDKQWGGIMMGDESYAGARSWEHLQTVVQNLTGMQYVLPTHQ
                     GRAAERILYSILGGKGKVFISNTHFDTTRANIEYVGSEAYDIVCKEAEDVESYHAFKG
                     NIDLEKLETLIQQHGKNIAAIIMTVTNNSSGGQPVSMKNMKDVRAICTKYGLMMVIDG
                     CRIAENSYYVKHREEGFENKTYKEIAQEMLNLGDAFIMSAKKDGMVNIGGLLTLKDEA
                     LSVKCKNLLIISEGFTTYGGLSGRDMEALAIGLEEVFDKDYLHYRIRSTAYLGEKLRE
                     KGIPVVWPIGGHAVYIDAKKLYQNVPLEEYPGQALVIDLYLKGGIRCVEVGSVMFGKY
                     DENGKLIPAANELVRMALPRRVYTQSHLDYVLDVFDDIIEIKDRKKGFKITYEPPFLR
                     HFTAHFEPIK"
     gene            complement(1699..2568)
                     /locus_tag="PLU36_03790"
     CDS             complement(1699..2568)
                     /locus_tag="PLU36_03790"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HMZ45902.1"
                     /translation="MFITLRTTVRSYFVGIGQIMLQENAITGLLFLIGIFYNSVIMGI
                     TIFFATIIGTSTAKLLKYDVNNINKGLYGFNAALVGIAGIVFLKIKILSISILIIACV
                     LSTILHNFFIKKKLPVYTLSFVTVTYSIIFFIKTFTPNLLILNEGCSPAYNLNFDYIF
                     KGFGQVIFQENAVSGIVFFIAIFINSPIAAIYAITFSALSGLIAISLSIPLNLVNAGL
                     FSYNALLCAIAFANRKLFTIFWVLLASSLSLIFSLLMNQIQIIQLTLPFVLACFIVST
                     LKNRNHINKQLLN"
     gene            complement(2754..3182)
                     /locus_tag="PLU36_03795"
     CDS             complement(2754..3182)
                     /locus_tag="PLU36_03795"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HMZ45903.1"
                     /translation="MIVAKKFKWEAAHRLPWHIGKCKQLHGHSYKMIVEFEGEPNENG
                     MIIDFNDIKNMLDPLVKQIDHATIISKNDIELKEIFEAKQWKYFLLPYDSTAENLCAF
                     FLETIINENKQVLKENNISKVGIKIFETETAYAYSTKLVD"
     gene            3460..4296
                     /locus_tag="PLU36_03800"
     CDS             3460..4296
                     /locus_tag="PLU36_03800"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMZ45904.1"
                     /translation="MKIVPHLIFVTITLFVISAKKLHAQKAGNEIFKETCSACHTVGK
                     GKLIGPDLANVHNHLTLDYFTKFVKSSQSVIKSGDKYADSIFNAYGKMAMPDHPQLSE
                     EQIKSIYDYLKEASGGGAEEAIPEEELLPGDIERGRALFVGNLRFTNSGASCNSCHNV
                     NIAGLITGGALAKDLTQSVTRLTETGVAGVIKGLPYPQMKETYAMHPITPQEVADLTT
                     FLVYVDEKSAIKTGNYTGNYMLLGGGGGIVFLLSLYSFFWFKRKRQAVNKSVFSRQIK
                     SA"
     gene            4443..8060
                     /locus_tag="PLU36_03805"
     CDS             4443..8060
                     /locus_tag="PLU36_03805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HMZ45905.1"
                     /translation="MSWIKDIISPQTRKWEEFYRNRFQHDRIVRSTHGVNCTGGCSWA
                     IHVKEGIVVFETQQLDYPLIDDKLPPYEPRGCQRGISYSWYLYSPLRIKYPLIRGTLL
                     DAFREEKQKVNGDPMKAWENLQNNKEQRKKYQNSRGKGGFRRTTWDEVLEIMAAANIH
                     TAKKYGPDRVIGFSPIPAMSMLSYASGARFLQLFGGVNLSFYDWYCDLPPAFPEIWGE
                     QTDVCESADWYNAKMIADMGTNLNMTRTPDCHFFAESRHNGTKTVVFSPDFSQLCKYA
                     DQWVPLHAGSDGAFWMSVTHVILKEYHFEKQTPYFIDYVKRYTDSPMLVHLTKTENGF
                     TAGRMVRANELSKWKNIENGDWKFLSIDEGTGEFVVPKGTMGYRWDKAGGKWNMKYEC
                     GETDKSYNPILSFINNNDDVLEVTFTEFGLSKTAERGVPVKWLDTENGRVPVTTVYDL
                     TMAQYGVDRGLKGDYPTDYNNPDAAYTPAWQEIFTGIDSKTLLQFAREWAETANTTEG
                     KCMILVGAGVNHWYHQNLTYRAGAMALMVTGCVGKNGGGLNHYVGQEKLAPVDSWGSL
                     AFAKDWVPVSRLQQAPLWHYINTCQYRYDGHHSNYNTTNKNKWTDKHVADTIFTSVRN
                     GWMPFYPQFNENSLELSKQAIANGATNDDEIKAYVLEKLKSKELKYAVSEPEAEENFP
                     RLWYIWRGNAIVGSMKGHEYALKHYLGTHTNVIAQDVDDKPEEIKWHNIAPVGKMDLV
                     VDLNFRMDSSALYSDIVLPAASWYEKADLNSTDLHSFIHPLGQAVAPVWESKTDWDIF
                     KHLAKATSEMAKKYFPEVQKDVVATPLSHDSVDEISQPTIKDWYIGECDAVPGKTMHK
                     ISVVERDYTDLYEKFITLGDAIKEKGLSAHGNHYLCKEEFDEMCQSQHFHQRKYKDKL
                     LPSIKEDEWAANAVLHLSSLTNGKLTKKAYEYMEKKTGLSLVDLSDDYLGVKIRYADL
                     LTKPHRYNNSPVWSGLMNNGRAYSAYTYNVERLVPWRTLTGRQHFYLDQEMYIAYDEH
                     LPTFKPAPSPEVYGDLRETWKNGKAKALNYLTPHGKWHIHSTYMENLRMLTLSRGIEP
                     CWLSEVDAEELGIKDNDWVEVHNDHGVYVTRACVSSRIPPGVCVVYHVPERTVGIPKS
                     QLRGNRRGGGHNSVTRIHLKPNYLNGGYGQFSYHFNYWGPVAPQRDTHVIVKKMEKLV
                     W"
     gene            8106..9656
                     /gene="narH"
                     /locus_tag="PLU36_03810"
     CDS             8106..9656
                     /gene="narH"
                     /locus_tag="PLU36_03810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HMZ45906.1"
                     /translation="MDVRSQIAMVFHLDKCIGCHTCSIACKNIWTDRKGAEYMWWNNV
                     ETKPGTGYPTKWEDQEIYKGGWEKRNDKIQLKGAGKRRGLTNIFYNPNLPVIDDYYEP
                     FTYKYLDLIESPETDDQPTARPVSLITGQPIDIKMGPNWDDDLSGSPDYARNDPNFKG
                     MTAEEQEVMFQLEKMAFFYLPRICNHCLNPACVASCPSGAIYKRGEDGVVLINQEVCR
                     AWRMCVTACPYKKSYYNWHTGKSEKCILCYPRMEAGLAPACMHSCVGRIRYLGVLLYD
                     ASKIEATASVDEKDLINSQMDMLLDPFDENVIREAKKNGVADSTIHAAQNSPIYKFVK
                     EWKLALPLHPEFRTLPMLFYVPPLLPVMATVKKTDNTEQQDKMHPIAKKWEDNWLYDT
                     STEELWGTLDQIRFPLKYIANLFSAGDEELIKEKWKKLMAVRIHRRKVTVGDISDEKV
                     ATTLRDAGMTAEEADAIYALTSLPKFEERFVIPAAHREQAIEMMDFTGDTKGDAGFGI
                     KTKPVRGI"
     gene            9689..10414
                     /locus_tag="PLU36_03815"
     CDS             9689..10414
                     /locus_tag="PLU36_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45907.1"
                     /translation="MEMVQNNNTSTANDLANYELLASLFLYPDNNQYKNGIEKVYNYL
                     NNSFPEAAITLQPFVEFINNNSAVEIQELYLRSFDVQAITTLDIGFVLFGEDYKRGKL
                     LVHLNEEHNKVGNQCETELSDHLPNLLKLLPKIGDDEVRNEIATLLIIPAVEKMISEF
                     SIEKIEKKDTVYKKHQKVLLDYSQNYRTIFQSTLMALFMVLQRDFGYNIIDTNGSTGS
                     LMDEPTEIKDFSVNIETEMLTEK"
     gene            10450..11178
                     /gene="narI"
                     /locus_tag="PLU36_03820"
     CDS             10450..11178
                     /gene="narI"
                     /locus_tag="PLU36_03820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HMZ45908.1"
                     /translation="MNLFDNFILVVLPYVAIATFLIGSIYRYTSRKFQVSSLSSQFLE
                     GKKLFYGSVPFHWGIVFIFLGHLIAFLFPRGVLAWNSQPVRLMILEISAFMFGISMLV
                     GLINLFIRRYTTPRLRYVTNYMDFAIYVLLILQTFAGLWIAYNYRWGSSWFSTLLTPY
                     LYSIFKFNPETSAIVLLPLMVKLHIAGAFIIIGILPFSRLIHLLVLPLNYIWRPYQQV
                     MWYWDKNKVRSHKTPWSLQRPKNT"
     gene            11181..11627
                     /locus_tag="PLU36_03825"
     CDS             11181..11627
                     /locus_tag="PLU36_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45909.1"
                     /translation="MFLSIVSDEQLMTNQIVKSILLIILSGGIVLNIFRIFRNPTRNK
                     KLLHIVLLLIISTIFYFVFNAYKIEAALLKNPQYIIGTTKGFCKTYGQSNIDFEYEIN
                     GKKFEGCNTYSPIPQSSIITNGGRYLVRYSNKYPEGGRMDFTKPAK"
BASE COUNT         4201 a         1803 c         2001 g         3689 t
ORIGIN      
        1 gttaataaat ctatatatac atcttctgca ggaagaagaa atgtattaaa accagcttct
       61 tttaaagctt ttaaacgttg tgggcgggta gtaactttta aaagttctac catcttcatt
      121 ctataaggtt ctgcccatga tctttttgta atttttgcca tattattatt ttataggttc
      181 aaagtgtgcg gtgaagtgtc ttaagaaagg aggttcgtaa gtaattttaa atcctttttt
      241 tctatctttt atttctataa tatcatcaaa tacgtctaat acataatcta agtgactttg
      301 tgtataaacc cttctaggta atgccattct tacaagttca ttggcagcag gaattagttt
      361 tccattttca tcatatttac caaacattac acttcccact tctacacaac gaattccacc
      421 ttttaagtat aaatcaatta ccaaggcttg ccctggatat tcttctaatg gtacattttg
      481 atataatttt tttgcatcaa tataaacagc atgcccacca ataggccata caacaggaat
      541 acccttttct cttaattttt ctcctaaata agctgtgcta cgaatacggt aatgcaaata
      601 atctttatca aacacttctt ctaagcctat ggctaaggcc tccatatctc taccagatag
      661 gcctccataa gttgtaaagc cctcggaaat gatgagtaag tttttacatt ttacagataa
      721 ggcttcatct tttagtgtta gtaagccgcc aatatttacc attccatctt tttttgcact
      781 cataataaat gcatcgccta gattaagcat ttcttgtgct atctctttgt aggttttatt
      841 ctcaaagccc tcctctctat gttttacata ataactgttt tctgcaattc tgcaaccatc
      901 aatcaccatc attaaaccat atttagtaca aattgctcta acatctttca tgtttttcat
      961 gcttacaggc tggccaccag acgaattgtt agttacagtc ataataattg cagcaatgtt
     1021 tttaccatgt tgctgaatta gagtttctaa tttttctaaa tcaatattac ctttaaaagc
     1081 atgataagat tctacatctt ctgcctcttt acaaacaata tcataagctt cactacctac
     1141 atattctatg tttgccctgg ttgtgtcaaa atgtgtatta gaaataaata cttttccttt
     1201 accacctaaa atactataca aaatacgttc tgctgctcta ccttggtgtg taggtaaaac
     1261 atattgcatt ccggtaaggt tttgtactac ggtttgtaaa tgttcccaac ttcttgcacc
     1321 agcataactt tcgtctccca tcattattcc tccccactgt ttgtcactca ttgcacctgt
     1381 accactatcg gttaataaat caataaaaac atcgtccgac cttaataaaa atggattata
     1441 acccgccttt tctaaagcat tttgcctata ttcttctgta gtaatacgca agggttcaac
     1501 catctttatt ttaaatggtt ctgttagtgt tctcattttt ttactcatat aaattgttat
     1561 aaagggatga aaagagatag atttttttgg gtggcaaaga tagtagatta attaaaaaag
     1621 acatcaaagt cttaatttaa ttttataaac ggaaagtata ataataaatt taataaatat
     1681 tacacagtac tacaatggtt aatttaataa ttgcttatta atatggtttc tatttttaag
     1741 agtagaaaca ataaagcatg ctaatacaaa cggaagtgta agttgaataa tttgtatttg
     1801 attcataagc aaactaaaaa ttagcgaaag ggaagaggct agcaaaaccc aaaaaatagt
     1861 aaatagtttt ctattagcaa atgcaatagc acataacaat gcattgtagc taaatagccc
     1921 agcattaaca agatttaaag gaatggaaag actaattgca attaaaccag ataacgccga
     1981 aaatgtaata gcataaatgg cagctatagg agaattaata aaaatagcaa taaaaaaaac
     2041 tataccagat actgcatttt cttgaaaaat tacttgacca aaccctttaa aaatatagtc
     2101 aaaatttaaa ttgtatgcag ggctacagcc ttcgtttaat attaaaagat ttggtgtaaa
     2161 tgtttttata aaaaaaataa tactatatgt aacagttaca aaagataatg tgtaaacggg
     2221 taattttttt ttaataaaga agttgtgtaa aattgtggat agtacgcatg caattattaa
     2281 aatagaaata gataagattt ttatttttaa gaatacaata ccagcaatgc ctactagtgc
     2341 agcattaaac ccataaagcc ctttatttat attatttaca tcatacttta atagtttggc
     2401 tgtgcttgta ccaattattg ttgcaaaaaa tattgtaatg cccattataa ccgaattgta
     2461 aaatattcct attaaaaaca acaatcctgt aattgcattt tcttgtaaca taatttggcc
     2521 aataccaaca aaataagacc taacagttgt tctaagagtt ataaacatat aaagttgtaa
     2581 ttgcttacaa tgaagttact aaaacaatga atttaaacta aactatattc agttttagaa
     2641 ttgtaatttt attaattcta attaagaaat tgtaaacatt gtgttttaaa aaggaatgta
     2701 aactttagca aagtaggtat cctacattat actaatttaa aaaataaaaa catttaatca
     2761 actaatttag tagagtaggc ataggcggtt tctgtttcaa aaatttttat acccactttt
     2821 gaaatgttgt tttcctttaa aacctgttta ttttcattta ttatggtttc taaaaaaaag
     2881 gcacataaat tttctgcagt agaatcgtag ggtaataaaa aatacttcca ttgtttggcc
     2941 tcaaatattt cttttaactc tatatcgttt ttagaaatta tagttgcatg atctatttgt
     3001 ttaaccaagg ggtctaacat atttttaatg tcattaaaat caataatcat tccgttttca
     3061 tttggctccc cttcaaactc aacaatcatt ttataagaat ggccatgtaa ttgtttacat
     3121 ttacctatat gccaaggtag cctgtgtgca gcctcccact taaatttttt tgctacaatc
     3181 atttatagtt atactatttt aaaagaatat gcaaaattgt aagttttttt cttaaaacaa
     3241 tatgtaaact tttgttttta ctgcatatat gccttctatt attatcaaaa tataagggta
     3301 tttatttaaa aaaaatattt tggttctatt tcgcttcggg atatatttgt ctttgattac
     3361 gaaatcggga aaaaattaaa tggaaatagt acaatagcaa gttaatatca aaagccagga
     3421 gatgtaacaa ttttttaaag ctgtaaaaac gaacacagta tgaaaatagt tcctcactta
     3481 atttttgtaa caataacttt atttgttatt tcggctaaaa agctacatgc ccaaaaagca
     3541 ggaaacgaaa tttttaaaga aacctgctct gcctgccaca ctgttggaaa aggaaaactt
     3601 ataggcccag atttagccaa tgttcacaac cacttaacat tagattattt tacaaaattt
     3661 gtaaagtcat ctcaatcggt aataaaatct ggagataaat atgccgattc aatttttaat
     3721 gcttatggta aaatggcaat gccagatcat cctcaacttt ctgaagaaca aataaaatct
     3781 atttatgatt atctaaaaga agctagtgga ggtggtgcag aagaagcaat accagaagaa
     3841 gaattattac caggagatat tgaaagaggc agggctttat ttgttggtaa cttaagattc
     3901 actaactctg gtgcatcttg caactcttgt cataacgtaa atattgcagg ccttattaca
     3961 ggcggtgctt tagcaaaaga tcttacacaa agtgttacca gattaactga aacaggtgtt
     4021 gcaggcgtta taaaaggatt gccttaccca caaatgaaag aaacgtatgc aatgcatcct
     4081 attacaccac aagaggttgc agacttaact acatttttag tatatgttga tgaaaaatcg
     4141 gcaattaaaa ctggtaatta cacaggtaac tatatgcttt taggtggcgg tggtggtata
     4201 gtatttttat taagccttta ctcatttttt tggtttaaaa gaaaaaggca agctgtaaac
     4261 aaatcggtat ttagcaggca aataaaatca gcttaaataa aaattaagga ataaaaaaat
     4321 accaaaccta acccttaaaa aataactttt tcggcagcaa agtttaagaa actatagaac
     4381 taattaaaga attgaaagat tttaaggttt aaaaatattc attcatctat aataataaaa
     4441 acatgagctg gataaaagac atcatctctc ctcaaacacg caaatgggaa gaattttata
     4501 gaaacagatt tcaacatgat agaattgtta gaagtacaca tggggtaaat tgtactggtg
     4561 gctgctcttg ggctatacat gtaaaagaag gaattgtggt atttgaaaca caacagttag
     4621 attatccttt aatagatgat aaacttccgc cttacgaacc aagaggttgt caacgaggca
     4681 tttcttattc gtggtattta tatagtccat tacgtataaa atatcctttg ataagaggta
     4741 ctttattaga tgcttttaga gaagaaaaac aaaaagtaaa tggagaccca atgaaagcct
     4801 gggagaattt acagaataat aaagagcaaa gaaaaaaata ccaaaattca agaggtaagg
     4861 gtggctttag aagaacaaca tgggatgagg ttttagaaat tatggctgca gccaatattc
     4921 atactgctaa aaaatatggc cccgataggg ttattgggtt ttcacccatt ccggcaatgt
     4981 ctatgttaag ctatgcatcg ggtgccaggt ttttacaatt atttggaggt gtaaatctta
     5041 gcttttacga ttggtattgc gatttacctc cagcctttcc tgaaatatgg ggcgagcaaa
     5101 cagatgtttg cgaaagtgcc gattggtaca acgcaaaaat gattgctgat atgggtacca
     5161 atttaaatat gactagaaca ccagattgcc acttctttgc agaatctaga cataatggta
     5221 ccaaaactgt agttttctct cctgatttta gccaattatg caaatatgca gatcaatggg
     5281 ttcctcttca tgctggcagc gatggtgcat tttggatgtc ggttacacat gtaattttaa
     5341 aagaatacca ttttgaaaaa caaacaccat actttattga ttatgtaaaa agatataccg
     5401 atagccccat gttggtacat ctaacaaaaa cagaaaatgg ctttactgct ggcagaatgg
     5461 ttagagcaaa tgaattaagt aaatggaaaa atatagaaaa tggagactgg aaatttttat
     5521 cgatagatga aggcacagga gaatttgtag ttcctaaagg caccatggga tatagatggg
     5581 ataaagcagg tggaaaatgg aatatgaaat atgaatgtgg cgaaacagat aaaagttaca
     5641 atccgatatt aagttttatt aataataatg acgatgtttt agaagtaaca tttactgaat
     5701 ttggtttatc taaaacagct gaaagaggtg ttcctgtaaa atggcttgat acagaaaatg
     5761 gaagagtacc agttaccaca gtgtatgatt taaccatggc tcaatatggt gtagacaggg
     5821 gtttaaaagg agattaccca acagactata ataatccaga tgctgcatac acaccagcct
     5881 ggcaagaaat atttacaggc attgattcta aaactttatt acaatttgca agagagtggg
     5941 ccgaaactgc taatacaaca gaaggaaaat gtatgatatt agttggtgct ggtgttaatc
     6001 attggtacca tcaaaaccta acttatcgtg caggggcaat ggcattaatg gttactggtt
     6061 gtgtaggtaa aaatggagga ggtttaaatc actatgtggg gcaagaaaaa ttagcaccag
     6121 tagattcttg gggttcttta gcttttgcta aagattgggt gcctgtttca aggttacaac
     6181 aagcaccact ttggcattat attaatactt gccaatatag gtatgatgga caccattcaa
     6241 actacaacac tactaataaa aataaatgga cagataaaca cgtggcagat accatattta
     6301 catctgtaag aaacgggtgg atgccatttt atcctcaatt taacgaaaac tcattagagc
     6361 tatccaaaca agcaattgcc aatggtgcta caaatgacga tgaaataaaa gcatacgttt
     6421 tagaaaaact aaaatctaaa gaacttaaat atgctgttag cgagccagaa gcagaagaaa
     6481 actttcctag gctttggtat atatggcgtg gcaatgcaat agttggtagt atgaagggac
     6541 atgaatatgc actaaagcat tatttaggaa cccatacaaa tgttattgca caagatgtag
     6601 atgataagcc agaagaaata aaatggcaca atatagcacc agtaggtaaa atggatttag
     6661 tagtggattt gaattttaga atggattcat cggcactata ctcagatatt gttttacctg
     6721 cagcatcttg gtacgaaaaa gcagatttaa acagtaccga tttacattct ttcatacatc
     6781 cattagggca agcagtagca ccagtttggg agtcgaaaac agattgggat atttttaaac
     6841 atttggctaa agcaactagc gaaatggcaa aaaaatattt tccagaagtg caaaaagatg
     6901 ttgttgcaac acctttatct cacgacagtg tagatgaaat ttctcagcca accattaaag
     6961 attggtatat tggagagtgt gatgcagtgc ctggtaaaac tatgcataaa ataagtgttg
     7021 tagaaagaga ctatacagat ttgtatgaga aatttatcac cctcggagat gcaattaaag
     7081 aaaaaggatt aagtgcacat ggaaaccatt atttgtgtaa agaagaattt gatgaaatgt
     7141 gccaatctca acatttccat caaagaaaat ataaagataa actactaccc tctattaaag
     7201 aagatgaatg ggctgccaat gcagtcttac acctttcttc tttaaccaat ggcaaactaa
     7261 ccaaaaaagc ctatgagtac atggaaaaga aaacaggctt atcgcttgta gatttatctg
     7321 atgattattt aggagtaaaa attagatatg ccgatttatt aactaaacca catagataca
     7381 acaattcacc tgtttggtcg ggtttaatga ataatggaag agcatactct gcttacacct
     7441 ataatgtgga aaggctagta ccttggagaa cattaactgg tcgtcagcat ttttatttag
     7501 accaagaaat gtacatagcg tacgatgaac atttaccaac atttaaacct gcaccaagcc
     7561 cagaagtgta tggagatttg agagaaacct ggaaaaatgg aaaagcaaaa gcattaaatt
     7621 atttaacacc acatggtaaa tggcatattc actctaccta catggaaaac ttgagaatgt
     7681 taaccttatc aagaggaata gaaccttgtt ggttaagtga ggtagatgct gaagaactag
     7741 gcattaaaga taacgactgg gtagaagttc ataacgacca tggtgtgtat gtaacaagag
     7801 cctgcgtaag ctcaagaatt ccaccaggtg tatgtgttgt atatcatgtg ccagaaagaa
     7861 cggtaggtat tccaaaatcg caactaagag gcaatagaag aggaggaggg cataatagtg
     7921 ttacacgtat tcacttaaaa ccaaattatt taaatggcgg ttatggccag ttttcctatc
     7981 atttcaatta ttgggggcct gtggcaccac aaagagatac ccatgtaatt gttaaaaaaa
     8041 tggaaaaact agtttggtaa aaataatagc agtatttaaa attcatcatt caaaactata
     8101 aactcatgga tgtacgatca caaatagcta tggtatttca cttagataaa tgtatcgggt
     8161 gtcatacatg ttctattgcc tgtaaaaata tttggacaga tagaaaaggt gcagaatata
     8221 tgtggtggaa taatgtagaa accaaaccag gtacaggcta tccaactaaa tgggaagatc
     8281 aggaaattta taaaggaggc tgggaaaaaa gaaacgacaa aatacaatta aagggtgcag
     8341 gcaaaagacg tgggctaacc aacatttttt ataaccccaa cttacctgtt attgatgatt
     8401 attatgaacc ttttacgtat aaatatttag acctaataga gtctccagaa acagatgacc
     8461 aaccaacagc tcgccctgtt tcattaatta ctggtcaacc aatagatatt aaaatgggac
     8521 ctaactggga tgatgactta agtggctctc cagactatgc cagaaatgat ccaaacttta
     8581 aaggcatgac tgcagaagaa caagaagtaa tgtttcagtt agaaaaaatg gccttctttt
     8641 atttgccaag aatttgtaac cattgcctaa atcctgcctg tgttgcatct tgcccatctg
     8701 gagcaattta caaacgtggc gaagatggag tagtattaat caatcaagaa gtgtgcagag
     8761 cttggagaat gtgtgttacg gcatgccctt ataaaaaatc ttattacaac tggcatacag
     8821 gaaaatcaga aaaatgtatt ttatgttatc caagaatgga ggcaggttta gctccagcat
     8881 gtatgcactc ctgcgttgga agaattagat atttaggagt tttactttac gatgcttcta
     8941 aaatagaagc tacagcaagt gtagatgaaa aagatttaat caatagccaa atggatatgc
     9001 ttttagatcc atttgatgaa aatgtaatta gagaagcaaa gaaaaacggc gtagcagatt
     9061 ctaccattca tgcagcacaa aactcaccaa tatacaaatt tgtaaaagaa tggaaattgg
     9121 ctttgccatt gcacccagag tttagaacat taccaatgtt attttatgtt ccaccattgt
     9181 tacctgtaat ggcaactgtt aaaaaaacag ataatacaga acaacaagat aaaatgcatc
     9241 caattgcaaa aaaatgggaa gataattggt tgtatgatac aagtaccgaa gagctttggg
     9301 gtacattaga tcaaattcgt tttccattaa aatatattgc caatttattt agtgctggtg
     9361 atgaagaact aataaaagaa aaatggaaaa aattaatggc cgttcgtatt catagaagaa
     9421 aagtaacggt tggagatatt agtgatgaaa aagttgcaac aacacttaga gatgcaggca
     9481 tgactgctga agaagcagat gctatatatg ctttaacatc attaccaaaa tttgaagaaa
     9541 ggtttgttat tccagcagct catagagaac aagcaataga aatgatggat tttacaggag
     9601 acactaaagg cgatgcagga tttggtatta aaacaaaacc agtacgaggc atttaaaaat
     9661 aatttaagag taaataaaat atgtagctat ggaaatggta caaaataata atacctctac
     9721 tgccaacgat ttggcaaact acgagttgtt agcatcctta tttttatatc cagataataa
     9781 ccagtataag aatggaattg aaaaagtata taattattta aacaatagtt ttccggaagc
     9841 tgcaataacc ttacaacctt ttgttgaatt tattaataac aattcagctg tagaaattca
     9901 agaactttat ttacgatctt ttgatgttca ggctatcact actttagata ttgggtttgt
     9961 tttatttggc gaagattata aaagaggaaa attgcttgtt cacttaaacg aagaacataa
    10021 taaggtgggc aaccaatgcg aaacagaatt atccgaccat cttcctaatt tattaaaact
    10081 attacctaaa ataggagatg atgaagtgag aaatgaaata gcaaccttgc taattatacc
    10141 tgcagttgaa aaaatgatta gtgaattttc gatagagaaa atagaaaaga aagatacggt
    10201 atataaaaaa caccaaaaag tattgctaga ctattctcaa aattatcgaa caatatttca
    10261 atccacatta atggctttgt ttatggtttt acaaagagac tttggatata atattattga
    10321 tacaaacggc agcactggtt cgttaatgga tgagccaaca gaaataaaag attttagtgt
    10381 aaacattgaa acagaaatgc tcacagaaaa ataaattaaa acattttata aataataaaa
    10441 ctacaggcta tgaacctttt tgataatttc attttagttg tattgcctta tgtggctata
    10501 gcaacttttt taattggatc tatctataga tatactagta gaaagtttca agtctcttct
    10561 ttatcttctc aatttttaga aggtaaaaag ttattctatg gctcagttcc ttttcactgg
    10621 ggcattgtat ttattttttt agggcactta attgcatttc tttttccaag aggtgtttta
    10681 gcctggaaca gccaacctgt taggttaatg attttagaaa tatctgcttt tatgtttggt
    10741 ataagtatgt tagttgggct tattaattta tttataagaa gatatactac accacgttta
    10801 aggtatgtaa caaattacat ggattttgcc atttatgttt tattaattct acaaacattt
    10861 gcaggcttgt ggattgctta taattaccgc tggggctcat cgtggttttc tactttacta
    10921 actccttatc tatattcaat ttttaaattt aaccccgaaa cttctgcaat agtattgttg
    10981 cctttaatgg ttaagttaca tattgctggt gcttttatta taataggtat tttacctttt
    11041 tcgagattaa tacatttatt agtgttgcca ttaaattata tatggaggcc ttatcaacaa
    11101 gttatgtggt attgggataa aaataaggtt cgtagccata aaacaccttg gtctctacag
    11161 aggcccaaaa atacatagtc atgtttttaa gcattgttag tgatgagcaa ttaatgacta
    11221 atcaaatagt aaaatctatt ttactaataa tactaagtgg aggtattgtt ttaaatattt
    11281 ttaggatttt tagaaaccca acccgcaata aaaaactact tcacatagtt cttttactta
    11341 ttatttcaac aatattttat tttgttttta atgcatataa aatagaagca gcattactca
    11401 aaaatccgca atatattatt ggcacaacca aaggcttttg caaaacctat ggccaatcta
    11461 atatagattt tgagtatgaa ataaatggaa aaaaatttga gggttgcaat acatatagtc
    11521 ctattccaca aagtagtata ataactaatg gtggaaggta tttagttaga tattctaata
    11581 aatatcctga aggtggtaga atggatttta ccaaacctgc caaataaaaa cagctatatg
    11641 aacagcactt caaccccaag agcctcttat gctgcacttg tgttaagcac attt
//
LOCUS       DAOQPK010000048        11435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_2929_length_11435_cov_4.214851, whole genome shotgun sequence.
ACCESSION   DAOQPK010000048 DAOQPK010000000
VERSION     DAOQPK010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11435
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2929_length_11435_cov_4.214851"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..980
                     /gene="deoC"
                     /locus_tag="PLU36_03830"
     CDS             60..980
                     /gene="deoC"
                     /locus_tag="PLU36_03830"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HMZ45910.1"
                     /translation="MAVTQTNFTIPDFSISPKIDQIGVEERAKRFTARSIKTTSKLSG
                     LKLVLNMIDLTTLEGKDTNGKVKQLCYKAQHLHNNYPDLPTVAAVCVYPSMVKVAKNA
                     LGNSGVKVAAVATAFPSGQAPKEVKITDTKFAVDNGADEIDMVISRGKFLQGEYNFVF
                     DEIATIKEACGNARLKVILETGELVTYDNVRKASDIAMYAGADFIKTSTGKIQPAATM
                     PVTLVMLEAIRDFYYKTGKMIAMKPAGGISKSKAALHYLVMLQETLGSAWMSNHWFRF
                     GASSLANDVLMQLEKERTGVYQSANYFSID"
     gene            1044..2510
                     /locus_tag="PLU36_03835"
     CDS             1044..2510
                     /locus_tag="PLU36_03835"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMZ45911.1"
                     /translation="MAKEKSNTAKLDFKLEYAAAPESKSSAKINSKYGLFINGKFEKP
                     ESKKYFDTINPATEEKIATIAEANQTDVNKAVKAARTAYNNVWSKMPAKERAKYIYRI
                     ARMIQERARELAIIETMDGGKPIRESRDIDIPLAANHFFYYAGWADKLQYAFPNKKIA
                     PLGVAGQIIPWNFPLLMAAWKIAPALATGNTVVLKPAETTPLTALKLAEIIQESDLPP
                     GVVNIITGAGATGAAIVNHPDINKIAFTGSTDVGKIIQRAIAGTNKKATLELGGKAAN
                     IIFDDAPLDQAVEGVINGIFFNQGHVCCAGSRLFVQESVYNKVLQKLRDRIETLIVGD
                     PLDKNTDIGAINSKQQMDTILKYLKIGEKEGAVMHQSQCSIPKKGYFIRPTLFTNVAQ
                     SSRIVQEEIFGPVLTIQTFRTDDEVIEKANNTPYGLSAGVWTDKGSKIFNLTTKMRAG
                     VVWANTFNKFDPTSPFGGYKESGFGREGGLHGLGAYLK"
     gene            2542..3426
                     /locus_tag="PLU36_03840"
     CDS             2542..3426
                     /locus_tag="PLU36_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMZ45912.1"
                     /translation="MNRLQVLKTYKIYIGGQFPRTESGRYYTPLNKTGKPLANICLSS
                     RKDFREAVIAARNAFGGWSSRAAFNRGQILYRIAEMLEGRKEQFINELKQQGSSHNQA
                     ESEVNTAIDRLIYFAGWCDKFQQFSSSVNPVASSHFNFSTPEPTGVVSIIAPQSSSLL
                     GLVSVIAPIIAGGNTCIVLASEQKPLCGITFAEVINSSDVPSGVVNILTGKPSELVKY
                     FADHMDVNAMVYCENDKDTQKLIQDKAALNLKRVLLYNDINWNNSEAENPYLILDAQE
                     IKTTWHPIENIGGAKAGY"
     gene            3441..3863
                     /locus_tag="PLU36_03845"
     CDS             3441..3863
                     /locus_tag="PLU36_03845"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HMZ45913.1"
                     /translation="MKVFWLFILLFCCGQFLYASDTIIVHKDSRLDILTQKQIIINKR
                     SSMLTSTGQYKGFRLQIISTREREKAFNIKAELLTKYPEEKSYVLFQSPYFRVRIGNF
                     VKREDAEKFRKILNKTYKNGVFIVEDVIDYIPPPDEEF"
     gene            3875..5062
                     /locus_tag="PLU36_03850"
     CDS             3875..5062
                     /locus_tag="PLU36_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HMZ45914.1"
                     /translation="MQLKEKITLLAKQYSNEFIELRHHIHANPELSYEEFETCQFIQQ
                     KLTSFGIVSSTLATTGVVALIEGNNPSKRVIALRADMDALPIMEENDVPYKSKKNGIM
                     HACGHDVHTTCLLGAAKILQETKTEWEGTIKLIFQPGEEKNPGGASIMIKEGVLNNPK
                     PDGIIGMHVHPGLPIGKFSFRKGRVMASADEIYITINGNGGHAAAPHLTTDTVLIAST
                     IIVSLQQIISRNNNPTIPSVLSFCAINGGTATNIIPSEVKLMGTLRAMDETWRYKAHQ
                     LITKHATAIAESMGATINIKIDIGYPTVDNDEALTTTAWQLADDFWGKEHVEETELRM
                     GAEDFGYYSQQIPGCFFRLGVRNELKGIIHNVHTPKFNIDEQAIENGIAMMAWLGATT
                     AVK"
     gene            5253..6563
                     /locus_tag="PLU36_03855"
     CDS             5253..6563
                     /locus_tag="PLU36_03855"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HMZ45915.1"
                     /translation="MKFGGTSVGKPERMHQVATLISQNNQPKIIVLSALSGTTNSLIE
                     ICNLLEQGNRETAKQKIDELEIHYKNFIIDLLSKEEYQAKAYEFISEHFDFLNIILKI
                     SFSEALSKDILAQGELLSTKLFSTYLSEVGIHHTLLPALEFMTIDNNEEPQIGSIKVK
                     LTQLLQQHSNCTIFVTQGYICRNAKGEIDNLKRGGSDYTASLIAAAINASVCEIWTDI
                     DGLHNNDPRIVDKTYAIEQLSFDEAAELAYFGAKILHPACIWPAQHFNIPVKLLNTMQ
                     PSAFGTLINDNKPTKGVKAVAAKDGIIAIKVKSSRMLLAYGFLKRIFEVFEKYKTSID
                     MVTTSEVAVSVTIDNNTHLKKIILELSEFSSVELVFEQTIISIVGNEINAQNEIMPKI
                     FSAISQIPVTMVSYGGSGHNISLLVPSNLKNQTLKALNTGLFDL"
     gene            6722..7810
                     /locus_tag="PLU36_03860"
     CDS             6722..7810
                     /locus_tag="PLU36_03860"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HMZ45916.1"
                     /translation="MRRFTFNLIYTSLAILIFSCNKNESTVNPIPTSVSISLDKTSVT
                     ADGIDEIKVTVKDQDGNDISASSVIFVDGFAIKGSAVFFEANQQGTHKVYANKYNVIS
                     DTLTVTTNAPTAAKYSTKVLAEFYTATWCGWCPRMDYKLNNFMAANNNLIAVQIHSND
                     NLANRTVDSIMRNDYGVSSVPNVLINRTSFMQENGDVNNLNDSTEFKKFYQKRAVVGL
                     AINSTVNGNNLDITAKTGFDVTIKDSLKLVILVVENNKIAPQANYYNNNLSYPGNPFF
                     SAGDTINTFVHNGVYSLSPTSIKGITVPVANQTKDNEYIANYTVDINGLNSANLKVIA
                     FVYFANDQARKGILNVQWANAGQNKNYD"
     gene            complement(7817..8860)
                     /locus_tag="PLU36_03865"
     CDS             complement(7817..8860)
                     /locus_tag="PLU36_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HMZ45917.1"
                     /translation="MALLHNRISNKELKEKLMREVEPRTTISFYCYFHIINPQEFRDF
                     LYKNLSTLKVFGRIYVAKEGINAQISVPTNYFNELKTFLYRIEPLHNLRLNIAVDDDG
                     KSFWVLKIKVRDKIVADGITDDAFDMKNKGKYVNAFGMNKLLEDKNTIVIDMRNHYEY
                     EVGHFENAIEIPSDTFREQLPMAVEMMKGNENKNIVMYCTGGIRCEKASAYMLHKGFQ
                     NVFHLEGGIINYAKQIKEEGEPSKFIGKNFVFDNRLGERITSDVIAKCHQCGATCDEH
                     TNCKNNGCHLLFIQCKNCANKYAGCCSEACKETINMPEERQKELRKGTENGIMIFNKS
                     KARLRPKLNESKP"
     gene            complement(8991..9791)
                     /gene="pyrF"
                     /locus_tag="PLU36_03870"
     CDS             complement(8991..9791)
                     /gene="pyrF"
                     /locus_tag="PLU36_03870"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HMZ45918.1"
                     /translation="MNKSYLEQQIQQKKSYLCVGLDTDITLIPDHLQQLPNAIVTFNK
                     AIIDATKDYCVSYKINTAFYEALGTKGWEIMEETLHYIPNTHFKIADAKRGDIGNTSA
                     RYAKAFFETLPFDAVTVTPYMGEDSIKPFLEYENKFTIVLGLTSNKGANDFELLKIGE
                     QFLYEKVLEKVSQWGTPNNLMFVVGATQANELQQIRKIVPENFLLIPGIGFQGGSLKD
                     VSTNGFNKNVGILVNVSRAIIYAGTNQQFATDAKEIALEYQTEMSSYL"
     gene            complement(9830..10678)
                     /locus_tag="PLU36_03875"
     CDS             complement(9830..10678)
                     /locus_tag="PLU36_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45919.1"
                     /translation="MDEKIREKASLFITIIYLFLGLMLLPIAIGMLTALPEMIGFLGV
                     ALLISSILFFYSAIKQFASNAKQKKANANFIQQINKQNKITNIAKQHDTIVNENIDIA
                     IDCSSSLSYSINNNNSVIANWQLSNEDWIKFYKNEKKERITSIIIEVFWILLLGTILL
                     KNTRMADTLDALLVSGLIGGVYGFGKYFLTMKSISPDVNSTENKEICITNSSILINGK
                     LNIFNDETRWFKSAKLLDNTNPLVVEITFGWKTRRGNTYDEIRIPVNNKETANNIISS
                     LYKKNN"
     gene            complement(10879..>11435)
                     /locus_tag="PLU36_03880"
     CDS             complement(10879..>11435)
                     /locus_tag="PLU36_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HMZ45920.1"
                     /translation="IESKKVAIKEAEDLIKKYDKQSSNVKNNREYEAINKEMEMQQLE
                     MKLAEKHIKDANEEIADKAKALEIAKKNIATKEGVLNHKKEELEKIIADTEKEETQFR
                     KKSDSARKDVEERLLYSYDRIRKSYRNGLAVVPVERDACGGCFNSIPPQKQSEIRLHK
                     KVMVCENCGRILVDTDLNDSVTTK"
BASE COUNT         4117 a         1738 c         1788 g         3792 t
ORIGIN      
        1 gtatatggtg ttggtgtata caattcatat attacatttg atatttacaa aaaatgatta
       61 tggctgtaac acaaacaaat tttacaatcc ccgacttttc aatttctcct aaaatagacc
      121 aaataggagt agaagaaaga gcaaaacgct ttactgcaag aagtataaaa acaacttcta
      181 aattaagtgg tttaaagctt gttttaaata tgattgactt aacaacttta gaagggaagg
      241 acacaaatgg taaagtaaaa caactttgtt ataaggctca acatttacat aataattatc
      301 cagatttacc aaccgttgct gcagtatgtg tatatccaag tatggtaaaa gttgccaaaa
      361 atgctttagg aaactctgga gtaaaagtag ctgctgtggc tactgccttc ccaagtggac
      421 aggcaccaaa ggaagttaaa attacagaca ccaaatttgc tgtagataat ggtgccgatg
      481 agattgacat ggtaattagc cgtggtaaat ttttacaagg tgaatacaat tttgtttttg
      541 atgaaattgc aaccattaaa gaagcctgtg gaaatgcaag actaaaagta attttagaaa
      601 ctggtgaatt agtaacctac gacaatgtga gaaaagcaag cgatatagcc atgtatgcag
      661 gtgcagattt tattaaaacc tctaccggaa aaatacaacc tgcagctaca atgcctgtta
      721 cacttgtaat gttagaagct atcagagatt tttattataa aacaggaaaa atgattgcca
      781 tgaaacccgc tggtggcata agtaaatcca aagctgcatt gcattattta gtaatgttac
      841 aagaaacatt aggcagtgca tggatgagta atcattggtt tagatttgga gcaagcagct
      901 tagccaacga tgtactaatg caattagaaa aagaaagaac aggagtttac caaagtgcca
      961 actactttag tatagattga tactattatt actattaaaa ataattacac caataaccat
     1021 tagaaccttt taaatataaa agaatggcaa aagaaaaaag caatactgca aaactagatt
     1081 ttaaattaga atatgcagca gcaccagaaa gtaagagcag tgcaaaaatt aattcaaaat
     1141 acggtttgtt tattaatggt aaatttgaaa aaccagaaag caaaaaatat tttgacacca
     1201 taaaccctgc tactgaagaa aaaattgcaa ccatagccga agccaatcaa actgatgtaa
     1261 ataaagcagt aaaagcagct cgtacagcat acaataatgt atggagcaaa atgcctgcaa
     1321 aagaaagagc aaaatatata tatcgtattg caagaatgat tcaagaacgt gctagagaat
     1381 tagcgataat tgaaacaatg gatggaggta agcccataag agaaagtaga gatattgata
     1441 taccacttgc agctaaccat tttttttatt atgcaggttg ggcagataaa ttgcagtatg
     1501 cattccctaa taaaaaaata gctccattag gtgtagctgg ccaaattata ccttggaatt
     1561 ttccattact aatggctgca tggaaaattg cccctgcttt agccacagga aatacagttg
     1621 ttttaaaacc agcagaaact actccattaa cagcattaaa attagccgaa attattcaag
     1681 aaagtgattt gccaccaggt gttgtaaata ttataacagg tgcaggagct acaggtgctg
     1741 caattgtaaa tcacccagat attaataaaa ttgcatttac cggttctacc gatgttggta
     1801 aaattattca acgtgctatt gctggcacaa ataaaaaagc aactttagaa ttaggaggta
     1861 aagctgccaa cataattttt gatgatgccc ctttagacca agctgttgaa ggcgtaataa
     1921 atggtatatt ttttaaccag gggcatgttt gttgtgctgg ttccaggcta tttgtacaag
     1981 aatctgttta caacaaagta ttacaaaaat taagagatag aattgaaact ttaattgtag
     2041 gagacccatt agataaaaat actgatatag gtgccattaa ctctaaacaa caaatggata
     2101 ctattttaaa gtatttaaaa attggcgaaa aagaaggagc agtaatgcac caaagccaat
     2161 gcagtattcc taaaaaagga tattttatta gaccaacttt atttaccaat gttgcacaaa
     2221 gtagtagaat tgtgcaagaa gaaatttttg ggccagtact tactattcaa acttttagaa
     2281 cagatgatga agtaattgaa aaagccaaca acacacctta tggcttaagt gcaggagttt
     2341 ggacagataa aggctctaaa atttttaatc taaccactaa aatgagagca ggagttgtat
     2401 gggcaaatac ttttaataaa ttcgatccaa catctccatt tggtgggtat aaagaaagtg
     2461 gttttggaag agaaggtggc ttacatggct tgggtgctta tttaaaataa tttttatcat
     2521 atcattaaat aataattcaa aatgaaccgt ttgcaagttc ttaaaacata taaaatctat
     2581 ataggtggac agtttcctag aacagaaagt ggaagatatt atacaccact taataaaaca
     2641 ggaaaaccat tagccaacat ttgtttaagc agcagaaaag attttagaga agctgtaatt
     2701 gctgcaagaa atgcttttgg aggatggagt agtagagctg cttttaatag aggacaaatt
     2761 ctatatcgta ttgcagaaat gctggaagga agaaaagagc aatttattaa tgagttaaaa
     2821 caacaaggct catcacataa tcaggcagag agtgaagtta atactgcaat agataggtta
     2881 atttattttg caggttggtg cgataaattt caacagttta gcagttctgt taatccagtt
     2941 gctagttctc atttcaactt ttctacacca gaaccaacgg gagttgtaag tattattgct
     3001 ccacaaagtt cttcattatt aggcttagtt tctgttattg ctccaataat tgcaggtggt
     3061 aatacttgta ttgttttagc tagcgaacaa aagcctttat gtggtataac ttttgcagaa
     3121 gtaattaata gtagtgatgt cccttctgga gttgtaaata ttttaacagg caagccttct
     3181 gaattagtaa aatattttgc cgaccatatg gatgtgaatg caatggttta ttgtgaaaat
     3241 gataaagaca cacaaaagct aattcaagat aaagctgctt taaacttaaa aagagttcta
     3301 ttatacaacg atattaattg gaacaatagc gaagctgaaa atccatattt aattttagat
     3361 gctcaggaaa ttaaaacaac ctggcatcct attgaaaata ttggtggagc aaaagcaggt
     3421 tattaaaaaa taaaattaac atgaaagtat tttggctttt tatattattg ttttgttgtg
     3481 gtcagttttt atatgcctca gatactatta ttgttcataa agatagtagg ttagatatat
     3541 taactcaaaa gcaaataatt attaataaac gaagttctat gcttaccagc acagggcaat
     3601 acaaaggatt taggttacaa attataagca ctagagagag agaaaaagca tttaatatta
     3661 aagcagaatt acttacaaaa tatccagaag aaaaatctta cgtgttattt caatctcctt
     3721 actttagagt tagaatagga aattttgtaa aaagagaaga tgcagaaaaa tttagaaaaa
     3781 ttttaaataa aacatataaa aatggggtat ttattgtaga agatgtaata gattatatac
     3841 caccaccaga tgaagagttt taataaaaaa acctatgcag cttaaagaaa aaattacatt
     3901 actagctaaa caatatagca atgagtttat agaacttcgt catcatattc atgcaaatcc
     3961 agagctcagt tatgaagagt ttgaaacctg ccaattcatt caacaaaaat taacttcatt
     4021 tggtatagtt agtagcacac ttgctacaac tggtgtagtt gctttaatag aagggaataa
     4081 ccctagcaaa agagtaattg cacttagagc agatatggat gctttaccaa ttatggaaga
     4141 aaatgatgtg ccttacaagt caaaaaaaaa tggcattatg catgcatgtg ggcatgatgt
     4201 tcatacaact tgtttattag gggcagctaa aattttacaa gaaactaaaa cagagtggga
     4261 gggtactatt aaattaattt ttcaaccagg agaagaaaaa aatcctggtg gagcaagtat
     4321 catgataaaa gaaggggttt taaacaatcc caaaccagat ggaataattg gcatgcatgt
     4381 tcatccaggt ttaccaattg gtaaatttag ttttaggaaa ggaagagtaa tggcaagtgc
     4441 agatgaaatt tacatcacaa ttaatggtaa tggaggtcat gcagcagcac cacatttaac
     4501 aacagatacc gttttaattg cttctacaat tatcgtttca cttcaacaaa tcatttcaag
     4561 aaataataat cctactattc catcagtttt atctttttgt gcaataaatg gtggtacagc
     4621 tacaaatatt attcctagcg aagtgaaatt aatgggtact ttaagagcta tggatgaaac
     4681 ttggcgttac aaagctcatc aactaataac caagcatgca acagctattg ctgaaagtat
     4741 gggtgctaca ataaatatta aaattgatat tggctaccca actgtagata atgatgaagc
     4801 ccttactacc acagcatggc aacttgctga tgatttttgg ggcaaagaac atgtagaaga
     4861 aactgaatta agaatgggag ccgaagattt tggatattac tcacaacaaa ttcctggatg
     4921 tttttttagg ttaggagtaa gaaatgaatt aaaaggcatt attcataatg tgcatacacc
     4981 aaaatttaat attgatgaac aagccattga aaacggtatt gcaatgatgg cttggttagg
     5041 agcaactaca gctgttaaat aaacatccta cacacaaaac agcctaacta atactcctaa
     5101 aaaaaatttt aaaaattaaa tttggtagct aaatttgcaa ccaattaatt ttcaattaac
     5161 taagactatg ggggggggct atcaggcagt aattgtttgt aagcaatacc tatatataca
     5221 ttagaacaat aaaaatttta cagatgaaag taatgaagtt tggaggaact agtgttggta
     5281 agccagaaag aatgcatcaa gtggctacac taattagtca aaacaatcaa ccaaaaatta
     5341 tagttttaag tgcacttagt ggtacaacca actcattaat tgaaatttgt aatttattag
     5401 aacaaggaaa tagggaaaca gctaaacaaa aaattgatga actagaaatt cattacaaaa
     5461 attttattat agatttatta agtaaagaag aatatcaagc aaaagcttat gaatttataa
     5521 gtgagcattt tgatttttta aatatcatat taaaaatatc ttttagcgaa gcattaagca
     5581 aagatatttt agcacaagga gagttgctaa gtactaaatt gttctcaacc tatttaagtg
     5641 aagttggcat acatcatacc cttttaccag ctttagaatt tatgacgatt gataacaatg
     5701 aagaaccaca aataggaagt ataaaagtta aactcactca acttttacaa caacattcaa
     5761 actgtaccat atttgttaca caaggatata tttgtagaaa tgcaaaagga gaaatagata
     5821 atttaaaaag agggggcagc gattatacag cttcattaat tgcagctgca attaacgcat
     5881 ctgtttgcga aatttggaca gatattgatg gcttacataa caatgatcca agaatagtag
     5941 ataaaactta tgcaatagaa caactaagtt ttgatgaagc tgcagaacta gcctattttg
     6001 gagctaaaat tttacaccct gcatgtattt ggcctgcaca acattttaat attccagtta
     6061 aactattaaa tactatgcag ccatctgcat ttggtacctt aataaatgat aataaaccta
     6121 caaaaggtgt taaagctgtt gcagcaaaag atggcattat tgcaattaag gttaaaagta
     6181 gcagaatgtt gctggcatat gggtttttaa aaagaatttt tgaagtattt gaaaagtata
     6241 aaacttcaat agacatggtt acaacgtctg aagttgctgt gagtgtaact attgataata
     6301 atacacatct taaaaaaatt attttagaat tatcagaatt tagttcagtt gaattagttt
     6361 ttgagcaaac aataatatct attgttggca atgaaataaa tgctcagaat gaaattatgc
     6421 ctaaaatatt ttcagccatt tcccaaatac ctgttaccat ggtaagttat ggaggaagtg
     6481 gacacaatat atctttacta gtaccaagta atttaaaaaa tcaaactctt aaagcattaa
     6541 atactggttt atttgattta taactaatta gtgatattaa ttatcaaatg acaacttaac
     6601 ataatgttaa ttataggaaa tactctacct taaattaaat cttaaaaata aatggtaaaa
     6661 aattaaaatt aactgctttt tagcatcaca ataatttatg tttgcagcaa ctaataatta
     6721 catgagaaga tttactttta acttgattta cacatcgtta gccattttga ttttttcttg
     6781 caataaaaat gaaagtactg ttaatccaat accaacttca gtaagtattt cattagataa
     6841 aacttcagta actgccgatg gtattgatga aataaaagtt accgttaaag accaagatgg
     6901 aaatgatatt tctgcttcgt cggttatttt tgttgatggt tttgcaatta aaggctctgc
     6961 agtttttttt gaagctaatc aacaaggcac tcataaagtt tatgcaaaca agtacaacgt
     7021 aattagtgat acactaacag ttactacaaa tgcaccaaca gcagcaaaat attctactaa
     7081 agtattggca gaattttata ctgcaacctg gtgtggttgg tgccctagaa tggattataa
     7141 actaaataat tttatggcag caaataataa tttaattgct gttcaaattc atagtaacga
     7201 caatttggct aatagaactg ttgattctat catgagaaat gattatggag tttcatcggt
     7261 accaaatgta ttaattaatc gtacttcatt tatgcaagaa aatggcgatg taaataactt
     7321 aaatgatagt actgaattta aaaaatttta tcaaaaaaga gctgtagttg ggttagcaat
     7381 aaattcaaca gtaaatggca ataatttaga tattacagca aaaacaggat ttgatgtaac
     7441 cattaaagac agtttaaaac tagtaatttt agttgtagaa aataataaaa tagctccaca
     7501 agcaaattat tataataaca atttaagtta ccctggaaat ccattctttt ctgcaggaga
     7561 tacaataaat acttttgtgc ataatggtgt ttattctttg tctcctacaa gtattaaagg
     7621 aattactgtt cctgtagcca atcaaacaaa agacaatgag tacattgcaa attatacggt
     7681 agatattaat ggattaaact cggctaattt aaaagtaatt gcatttgtat attttgccaa
     7741 tgaccaagca agaaaaggaa tattaaatgt gcaatgggct aatgctgggc aaaataaaaa
     7801 ttacgattaa cctttactaa ggtttagatt cattcaattt gggccttaac cttgctttac
     7861 ttttattaaa aatcattatt ccgttttcag tcccttttct taattctttt tgcctttctt
     7921 ccggcatatt aattgtttct ttacatgctt cagaacagca tcctgcatat ttatttgcac
     7981 agtttttaca ttgtataaat agtaaatgac aaccattatt tttacaattc gtatgctcat
     8041 cgcatgtagc tccacattga tgacattttg caataacatc actagtaatt ctttctccta
     8101 gcctgttatc aaatacaaaa ttcttaccaa taaacttact gggttctccc tcttctttaa
     8161 tttgcttagc ataatttata attccacctt ctaaatgaaa aacattttga aatcctttgt
     8221 gcagcatgta agcactagct ttttcacatc ttattccacc agtacaatac ataacaatat
     8281 ttttattttc gtttcctttc atcatttcta ctgccatagg caattgttct ctaaaagtat
     8341 cgcttggaat ttcaattgca ttttcaaaat gtccaacttc atattcgtaa tgattacgca
     8401 tatcaattac tatagtgttt ttatcttcca acaatttatt cattccaaaa gcatttacat
     8461 acttgccttt atttttcatg tcaaaagcat catcggttat accatctgca acaattttat
     8521 ctctcacttt tatttttaac acccaaaagc ttttaccatc atcatcaact gcaatattta
     8581 gtcttaaatt atggagtggc tcaatacgat ataaaaatgt ttttaactca ttaaaataat
     8641 tagttggaac actaatttgt gcgttgatac cttctttagc tacatatatt cttccaaaaa
     8701 cttttaaagt agataaattt ttgtacaaaa aatctctaaa ttcttgtgga ttaataatgt
     8761 ggaaataaca atagaagcta atagttgtac gtggttcaac ttctctcatc agcttttctt
     8821 tcaactcttt attgcttata cgattgtgca ataatgccat aaataaaatt ttagacatcc
     8881 attttcatta caacttaatt ggtaaaaaac gaatgtttca gttaaacaat gaacccattt
     8941 gcaggatggg caaaattttg aagtacaaag gtaaataatt atacccgtaa ttataaataa
     9001 ctgctcattt ctgtttgata ttctaatgca atttctttag catcagttgc aaattgttgg
     9061 ttggttccag cataaattat tgccctgctt acattaacta atataccaac atttttatta
     9121 aagccgtttg tacttacatc ttttaaactg ccaccttgaa aaccaatacc tggaattaaa
     9181 agaaaatttt caggaactat ttttcggatt tgttgaagtt cgtttgcttg tgttgctcct
     9241 actacaaaca ttaaattatt aggagtgccc cattgagata ctttctctaa tactttttcg
     9301 tataaaaact gctcccctat ttttaataat tcaaaatcat tagctccttt attagaagta
     9361 agccctaaaa caatagtaaa tttattttca tactctagaa atggtttaat actgtcttcg
     9421 cccatgtaag gtgttacagt aacagcatca aatggaagcg tttcaaaaaa agcttttgca
     9481 tacctagccg atgtattacc tatatcgcct cttttagcat ctgcaatttt aaaatgcgta
     9541 tttggaatat aatgcaaggt ttcttccatt atttcccaac ctttagtacc taatgcttcg
     9601 taaaatgctg tattaatttt atagcttaca caataatctt ttgtggcatc tataatagct
     9661 ttattaaagg ttacaattgc attgggtaac tgttgtaagt ggtcaggtat taaagtaata
     9721 tcagtatcta accctacaca taaataagat tttttttgct gaatttgctg ttctaaatag
     9781 cttttgttca tacagcaaaa ctaaagtaga tttaggagtt gttcaataat taattgtttt
     9841 ttttgtagag agaggaaatt atattattgg cagtttcttt attatttaca ggaattctga
     9901 tttcatcata agtattacca cgtctggttt tccagccaaa ggtaatttct acaaccaagg
     9961 ggttggtgtt atctaaaagc tttgcacttt taaaccatct ggtttcatcg ttaaaaatat
    10021 ttaatttacc attaattaaa atactactgt tagtaatgca tatttcttta ttttctgtag
    10081 agtttacatc tggagaaata cttttcattg ttaaaaagta ttttccaaaa ccataaaccc
    10141 ctcctatcaa tccacttact agcaaggcat ctaaggtatc agccattctg gtatttttaa
    10201 gtaatattgt accaagtaac agtatccaaa acacttcaat aataatacta gtaatcctct
    10261 ctttcttttc atttttataa aactttatcc aatcttcatt acttaattgc caatttgcaa
    10321 ttacagaatt attattgttt atgctatatg ataaagatga actacaatct atagctatat
    10381 ctatattttc attaacaatg gtatcatgtt gtttagcaat gtttgttatt ttattttgct
    10441 tattaatttg ctgaatgaaa tttgcatttg cttttttctg tttagcattt gaagcaaatt
    10501 gcttaattgc agaataaaaa aacaaaatag atgaaataag taatgcaact ccaagaaagc
    10561 ctatcatttc tggtaatgct gtaagcattc ctatagcaat aggtaatagc attaatccta
    10621 aaaataaata gattatagtg ataaataaag aagctttctc tctaattttt tcatccatta
    10681 taaatcattt tattgttact acttacttgt ttattggcat gtgtctttgt aaaactaatc
    10741 ttattttttg aatcattcaa agcccatcta atttaaaaaa taaaaaatat aagtacccat
    10801 atttttcttg catttctaca aaaaaataga ggctactttt aaaggcagcc tccaagtaaa
    10861 ttttaattat tgtaaaactt atttagttgt tacactatca ttcaaatctg tatctactaa
    10921 tattctacca caattttcgc aaaccattac ttttttgtgt aaacgtattt cactttgctt
    10981 ttgaggagga attgagttaa aacagccacc acaagcatca cgttcaacag gtactacagc
    11041 taatccattt ctatagcttt ttctaattct atcgtagcta tataataaac gttcttcaac
    11101 atcttttctt gcagaatcgc ttttttttct aaactgtgtt tcttcttttt cagtatcggc
    11161 aataattttt tctagctctt cctttttgtg gttcaaaaca ccttctttgg ttgcaatatt
    11221 tttcttggca atttctaatg ctttggcttt atcagcaatc tcttcgttag catctttaat
    11281 gtgtttttca gcaagtttca tttctaattg ctgcatttcc atttctttgt ttattgcttc
    11341 gtactctctg ttatttttaa cattgctact ttgcttatca tactttttaa ttaaatcctc
    11401 tgcttcttta attgctactt ttttgctttc aataa
//
LOCUS       DAOQPK010000049        11319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_2962_length_11319_cov_4.706232, whole genome shotgun sequence.
ACCESSION   DAOQPK010000049 DAOQPK010000000
VERSION     DAOQPK010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11319
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2962_length_11319_cov_4.706232"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2107
                     /locus_tag="PLU36_03885"
     CDS             <1..2107
                     /locus_tag="PLU36_03885"
                     /inference="COORDINATES: protein motif:HMM:NF008897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HMZ45921.1"
                     /translation="WLYQLIHQDKKTIKSPKSYNSQVGVALSILNIETEHEIGIFEAG
                     ISTANEMDTLEKIIKPTIGILTNIGGAHNEGFEDDTHKFLEKIKLFKNASILIAREAD
                     FTNHYLKTINQFKPTLFTWGFSAKNEFVINSLIKQNISTTLSLQYKQQEMVFEIPFVD
                     DANIENVINCICLMLVLKYSSIIIKEKILKLQPVEMRMQLKKAINNCYVVNDTYSNDL
                     SSLKIALDYLQQQAGNQHTTVILSDILQSGINENELYQEVAALIKQKNIHKFIGIGEQ
                     LIKHKHFFENTIQQTIFYNTVDEFLIHSNLHQYKEEYILLKGARVFSFERIDEWFAYK
                     VHQTALEINLNAIVHNLKTYQNLLQAGTKVMAMVKAFSYGSGSIEIARLLQYHKVDYL
                     AVAYVDEGIELRKAGISLPIMVMNIDESGFNSVIENNLEPEIYSFPIYQAFHQYLQQQ
                     GITNFPIHLKLNTGMNRLGFDAFEVTAIATLLKQNNSMVVQSVFSHLTSSEDEKEDNF
                     TLQQFQLFQQLCNNIQNNLGYSFIKHIANSAAAIRHEHLQMNMIRLGIGLYGVDSSAS
                     KKIKLQTVAFLKTTIAQIRNLKKGDTVGYNRKEVLQRDSKIATIRIGYADGFSRKLGN
                     RVGSVFIKGKLAKIVGNVCMDMSMVDVTEIENVKEDDVVEIFGNNLPVQQVATWCQTI
                     SYEILTTISQRVKRIYLEE"
     gene            2120..2323
                     /locus_tag="PLU36_03890"
     CDS             2120..2323
                     /locus_tag="PLU36_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45922.1"
                     /translation="MKVYSFIIIAIILLSCLVILYKSFLIIKQGLNKEYNIKGRELIL
                     YYLLIGLTLLSVMERHFENILRD"
     gene            complement(2508..3083)
                     /locus_tag="PLU36_03895"
     CDS             complement(2508..3083)
                     /locus_tag="PLU36_03895"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HMZ45923.1"
                     /translation="MKRIAIFASGTGSNTAKIIHYFKDKSKGVEIALIVSNKPTAKVL
                     TIASDNNIATLIIDKEIFFRGNAYLPQLQNASIDYIVLAGFLWKIPINLIQAYPKKII
                     NIHPALLPKYGGKGMYGSYVHEAVIANKELESGITIHFVNEHYDEGEHIFKASCTVDK
                     FETVESLAAKIHALEYEHFAKVIDSVVNKLQ"
     gene            complement(3083..3367)
                     /locus_tag="PLU36_03900"
     CDS             complement(3083..3367)
                     /locus_tag="PLU36_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45924.1"
                     /translation="MFHKLKEKWNVNWLQFTLIFTTFALGGSSCAKLASYILQLFLSE
                     KDFIYWIIYLPMVTILWPICVIIISIPLFQFPFFKNYLKKMWTRIKGSTK"
     gene            3585..>11319
                     /locus_tag="PLU36_03905"
     CDS             3585..>11319
                     /locus_tag="PLU36_03905"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMZ45925.1"
                     /translation="MYNLYFHTLIKRGILLFFIFFTINTSGQNISPNADTTVCGSITL
                     TAPGSATYEWKQSINNGVSWTSIAGATAQTYTVTQSGLYTVITSPATLDTVLITVNPK
                     PVVGFTIVSNNLCPSVPVFFNNTTTSANNFLWYFDDINSGSNNTSTLNSPTHKFIGNA
                     GTGTQNYNVKLVASSNAGCKDSTTLTATVKQRPGTELNGTGSSTFNNLSFFKLCGTSS
                     SDFNFVNISSTTSTNTGYNIKWGDATPDANMPTFSAPITHNYSIGSHTLLYSVTGNNG
                     CTDTASYFVFVGSNPAVGLGNPGNTTICTKDSLTFPVNNTSGNTPGTMYTITINDNTA
                     PFSFNPIGVNPSFTHVFNISSCGVNSSDGANTYSNSYSARITASNPCGTSAANIVPIY
                     VSKKPEPAFSILPSDTTCVNKNITFTNTGGNNNYVDNGTCNNGKNVWSITPTTGWNIV
                     SGTIGDTFGFSDPSLWATGTNAIQIKFTSPGVYTIKLQAGNPKCGAAEIIKTVCVNPQ
                     PTANFTVDNSNGCAPLTVNTTNASSTPICGTNKFFWSVTYSPASGCTPNTSSYFYLDG
                     TSNSSQNPKLKFDNPGTYTIGLYIQNGDCTSSTVFKQITVKGKPSVSPINASNVCQNG
                     STSPTVTVGNCNTSGMPTYAWSFPGGNPTSSTQPTPGNVTYTTFGTPTISLDVTNECG
                     TTYVIKTITVSAAPTVVVPSNKTFCAGDATGMLNFSSSVSTANYTWTNSNTAIGLNAS
                     GSASSSNPFINSFIAQNNTASPITATITVTPVSGCSGIPQTFTITVNPKPTLPVVVSP
                     INYCLNEVATALNASATGGNTLMWYNNAALLNGTVTAYTPLTTSTGTTNYYVTQENSY
                     GCKSSAATISVTVVPIISNNTIGNNQTICAGATPNTLTQTSTTTGGTGVYTYQWQQST
                     DGGLTWSSIIGANTSSYLPIGISVNTQFRRIVLSACSDTSNVISITILGVLNNTGVSA
                     AQVICQGNTPAMLDGDTPNGGNGTFNFTWEQSTNNTTWSIISGITTEDYQPPALNVTT
                     YYRRKTESGSCAAYSASIKITVNPKPVITPIANATLCNSITTTPIVFTSTPNTGVTYV
                     WQNDNIDIGLPLTGSGNIAAYSTTNNSSPKVPIIGNISVVPTYTFSSVACLGDTGKFS
                     ITILPTISINAVADTTICSGSSLPAKTFIHDAGSYAETNVTYQYTISGSGTSLVSGTG
                     STVPAYTANNVGNTNLTTTVTVKPVYTFNGKSCDGIAETYTITITPSTPTAAAGNDTI
                     LCSANTYTMQATLAGGSTGVWSEFGGNSTTIQNTTSPTTQITGLLPNNTYKYVWNVTG
                     VASCPGTSDTITIINYASVVNQIDNTTKVICATQTININGNSPTGGSGSYTYQWQQST
                     DGGLTWTNIPFKTSKDLSYIPTTSVLIRRIVYSLPCIEQSSTTIVTVQPAISNNTISA
                     NQSICINTVPTTLIGTVATGGDGNISYQWQLSTNGGITWSDISGATSKDFSPSALTVT
                     TKYRRVVSTSLCFGTQGNNSLAITITVNPDSHANYTFTNNIGCAPFLINSINIKPTLL
                     PLQNGTYNWYANNTYLVGGPSFPGFTLTNPGDSIQIKLVTTSPFGCKADSVTHKFYTV
                     AKPQTSFTASDTIGCGPLNVLFTNTTPNINWFNYQWSFGNGLTSNLPQPNTVTFAINP
                     TFFDTIYTVQLSATTVCQTVKDTQYIRVKSKPKAVFTPNKSVGCSPMTVFFNNTSKGN
                     NVQYKWNFGDGGADTLVTTKSSMSYTYYRGFQDTVDVRLIAFNDCGSDTQTYKIVISP
                     NLIQLDFAVNGNETQGCKPKSVKFINASNGATNFFWSFGDGNTLSTTKNIDTVTHTYT
                     TAGTFIATLKASNNCSDTTSVETIQIFNTPIINFTAVPSSICIGDTIHFTNLTDTASS
                     YLWSFNDNSTSTLVNPKHIYPVAGNYLVQLKALRQYNSGLTCIDSISKPISVVSKLKG
                     SFTANDTISPCVPFTLSMVNNTPALQTIWYSNNIPIDTGNVATYTFNQVGSYLIKMNA
                     TNAAGCIKEDTQTVVVRGPSGTFQYDHGIICSNNAVYFEVLPSNTDSIKFNFGDGNFL
                     TTTANSVYHQYNQTGSFIPSVILYSNFSPACTVTLQGTDTIKIDYVKAGFKYSSQTYC
                     DSTKIFFNDTSRHALPIVSWQWNFGDGNFSTLQHPTHTYKNTNNWAVQLITKTVGGCT
                     DTVNYNMFVKVNNKPSNTILAPLVACALKPINYQAQTTSVDSIIFNNWQFNNGLTTLG
                     NNVNNTYILPGTYYATLISGTAYGCTDTALHTITINPTPTVVTTSNQIICLGSSIQLN
                     VSGANTYSWAPINNTLSCLTCINPIATPITSTMYIVEGTNTFGCSNKDSVMVTVAQPI
                     TIQTSANDTICIGQSAQLSATGATTYQWMPSSSLQNNNTANPIATPAITTTYQVIGFD
                     NYNCFQDTGFITVAVGKYPTITLEADKVLPTGTQLALKSTVTNGPITNWLWTPSADLN
                     CNTCALPIATIKNDVCYTVTAANIYHCAATDTICIKTFCEGTQVFIPNAFTPDGDGIN
                     DVLMVRGSGIKTVKSFRIFNRWGQVVFEKSNVTPNDPAQG"
BASE COUNT         3958 a         1853 c         1753 g         3755 t
ORIGIN      
        1 atggttgtat caactaattc atcaagataa aaagacaata aaaagtccga agagttataa
       61 ttcacaagtt ggtgtagcat tgtccatttt aaatatagaa acagaacatg aaataggaat
      121 ttttgaagct ggtatttcaa cagctaacga aatggatact ttagaaaaaa ttattaaacc
      181 aacaataggc atactaacca atattggtgg agcacataat gaagggtttg aagatgatac
      241 acataagttt ctggagaaaa taaaactttt taaaaatgca agtatattaa ttgcaagaga
      301 agctgatttt actaatcact atttaaaaac aataaatcaa tttaagccta ctttatttac
      361 ttggggattt tctgcaaaaa acgaatttgt aattaatagc ctaataaagc agaatatatc
      421 tactactcta tcattgcaat ataagcaaca agaaatggtt tttgaaatac catttgtaga
      481 tgatgccaat atagaaaatg taattaactg catttgttta atgcttgtat taaaatatag
      541 ttctatcata attaaagaaa agattttaaa attgcaacct gttgaaatga ggatgcaact
      601 taaaaaagct attaataatt gctatgttgt aaatgatacc tatagcaatg atttatcatc
      661 cttaaaaatt gcattagatt acttacaaca gcaagcaggc aaccagcata ctactgttat
      721 actatcagat attttacagt ctggcattaa tgaaaatgaa ttatatcaag aagttgcagc
      781 attaattaaa caaaaaaata ttcataaatt tattggcatt ggtgagcaac ttataaaaca
      841 caagcatttt tttgaaaata ccattcaaca aactattttt tataatacgg tagatgaatt
      901 tttaattcac tccaatttgc accagtataa agaggaatat attttactaa aaggtgccag
      961 agttttttca tttgaaagaa tagatgaatg gtttgcctac aaagtacacc aaactgcatt
     1021 agaaattaac ttgaatgcaa ttgtacataa tttaaaaaca tatcaaaatt tgttacaagc
     1081 tggtacaaaa gtaatggcta tggtcaaagc ttttagctat ggtagcggca gtattgaaat
     1141 agcaaggtta ctacaatacc ataaggtaga ttatttggct gtagcttatg ttgatgaagg
     1201 tattgaatta cgtaaagcag gtattagctt accaattatg gtaatgaata ttgacgagtc
     1261 tggctttaac tctgtaatag aaaataattt agaaccagaa atttattcct ttccaattta
     1321 tcaagccttt catcaatatt tacaacaaca aggcattact aattttccta ttcatttaaa
     1381 gctaaataca ggcatgaatc gtttagggtt tgatgcgttt gaagtaacag ctattgctac
     1441 actgttaaaa caaaataatt caatggtggt gcaatctgtt tttagtcatc ttacctctag
     1501 cgaagatgaa aaagaagata attttacttt acaacaattt cagctttttc aacaattgtg
     1561 caataacatt caaaataatt taggttattc ttttattaaa catattgcaa acagtgcagc
     1621 agctataagg catgaacatt tacaaatgaa tatgattcgt ttgggtattg gtttgtatgg
     1681 tgttgatagt tctgcatcta aaaaaattaa attacaaaca gttgcatttt taaaaactac
     1741 aattgctcaa ataagaaatt taaaaaaagg agatacagta gggtacaaca gaaaagaagt
     1801 gttacagcga gacagtaaaa ttgcaaccat tagaattggt tatgcagatg gttttagcag
     1861 aaagttaggc aatagagtag gcagtgtttt tattaaaggt aaacttgcta aaattgtagg
     1921 caatgtttgt atggatatga gtatggttga tgttacagaa attgaaaatg tgaaagaaga
     1981 tgatgtggtt gaaatttttg gaaataattt acctgtacaa caagtagcta catggtgcca
     2041 aactattagt tacgaaattt taacaaccat tagccaacgg gttaaaagaa tttatttaga
     2101 agaataaaat ataacaccaa tgaaagtata tagttttatt ataattgcaa ttattttatt
     2161 aagttgttta gttatacttt acaaatcatt tctaataatt aaacaaggat taaataagga
     2221 atacaatata aaaggcagag agttaatttt atactactta ttaattgggt taactttact
     2281 atctgttatg gagaggcatt ttgaaaatat attaagagat taaaacttta atatacgttc
     2341 cttcttcctt ttttctatct attataccaa gcaaatactg gatttttatt tattgcttga
     2401 aggctaacta tattaaattg aattattttt tttacgtttt ttaaagtaga aataaaagtt
     2461 cctaacaagg tttgtagggc attaaaatat tgctacaaac aagttgttta ttgtaactta
     2521 tttacaacac tatcaataac tttagcaaaa tgctcgtatt ctaaagcatg aatttttgct
     2581 gctaaactct caacagtttc aaacttatct actgtacaac ttgctttaaa aatatgctca
     2641 ccttcgtcgt agtgttcatt aacaaaatga atggtgatac cactttctaa ttctttatta
     2701 gcaataactg cctcgtgtac gtagctacca tacatacctt tgcctccata tttgggtaaa
     2761 agtgctgggt gtatattaat aattttttta gggtaagctt gaataagatt aattggaatt
     2821 ttccataaaa agcctgcaag aactatataa tcaatagaag cattttgtag ctgaggcaaa
     2881 taagcattac ctctaaaaaa tatttctttg tctataatta atgtggctat attattatca
     2941 gaagcaatgg ttaaaacttt tgcagtaggc ttattgctca caattaaagc tatttctaca
     3001 cctttacttt tatctttaaa ataatggata atttttgcag tattactgcc tgttccactt
     3061 gcaaaaatgg caatacgttt cattatttgg tactcccttt tattctagtc cacatttttt
     3121 ttaaatagtt tttaaagaat ggaaattgaa aaagtggaat actgatgatt attacacaaa
     3181 ttggccaaag tatagtaacc attggcaaat aaataatcca ataaataaaa tctttttcgg
     3241 ataaaaataa ttgtaaaata tagcttgcca gtttagcaca tgagctacca cccaaagcaa
     3301 atgttgtaaa aataagtgta aactgtaacc aatttacatt ccatttttct tttagtttat
     3361 gaaacataac tgcccgaaga tagggataga aataatatgt tttttataat caaaatcatt
     3421 aaaatttcct attttatttt tttcaggcat aaaataccca ataaatggta tcattaattg
     3481 ataaatattt tatttttttg aaggttgaag aatagttaat atatatttta tagttttaac
     3541 ttattctaaa aaatacataa ctctatttta atcaaaacct gcacatgtat aatttgtatt
     3601 ttcatacatt aataaaacgg ggaattttat tattttttat tttttttaca attaatacta
     3661 gtggtcaaaa catttctcca aatgccgata caacagtttg tggaagtatt actttaacag
     3721 caccaggttc tgctacttat gaatggaaac aaagtataaa taatggagta agctggacta
     3781 gtattgctgg tgcaacagct caaacctata ctgttactca atctggttta tatacagtaa
     3841 ttacttctcc tgcaacttta gatactgttt taataactgt aaatcctaaa cctgttgttg
     3901 gctttacaat tgtgagcaat aatttatgcc ctagcgttcc agtgtttttt aataatacca
     3961 ctactagtgc aaataatttt ttgtggtatt ttgatgatat caattctggt agcaataata
     4021 catctacatt aaatagtcca acacataagt ttattggaaa tgctggtact ggcactcaaa
     4081 attataatgt aaagctagta gcatctagta atgcaggatg taaagacagt acaactttaa
     4141 ctgcaacagt aaagcaaagg cctggtacag agttgaatgg tacaggctct tctactttta
     4201 ataatttatc tttctttaaa ttatgtggta cttcgtcttc tgattttaat tttgtgaata
     4261 tatctagtac cacttcaacc aatactggtt ataatattaa atggggagat gcaacacccg
     4321 atgcaaatat gcctactttt tcggcaccca tcacacacaa ttattctata ggctctcaca
     4381 cattattgta ttcggtaaca ggtaataatg gttgtacaga tactgcttct tattttgttt
     4441 ttgttggtag taatcctgca gttggattag gcaatccggg gaatactaca atttgcacta
     4501 aagactcctt aacttttcct gtaaataata cttcaggtaa cactcctgga acaatgtaca
     4561 caattactat taacgataat acagccccat ttagttttaa tccaattggt gttaatccta
     4621 gctttacgca tgtatttaat atttcaagtt gtggtgtcaa tagttctgat ggtgccaata
     4681 cgtattctaa ttcttattct gcaagaatta cagcctctaa tccttgtggt acatctgctg
     4741 ctaatattgt acctatttat gtttctaaaa agccggagcc tgctttttct attttacctt
     4801 ctgatacaac ttgtgtaaac aaaaacatta cttttactaa tacaggtggt aataataatt
     4861 atgtagataa tggaacctgt aataatggta aaaatgtttg gtcaataaca ccaactactg
     4921 gttggaacat agtgagtgga actattggag atacttttgg tttttcagat ccctctcttt
     4981 gggctacagg tactaatgca atacaaataa aatttacaag ccctggtgta tatactatta
     5041 aactgcaagc aggtaaccct aaatgtggtg ctgcagaaat tattaaaaca gtttgtgtaa
     5101 atccacaacc cactgccaac tttactgttg ataattctaa tggctgtgca ccgttaactg
     5161 taaatactac caatgcatct agcactccta tttgtggtac aaataagttt ttttggtcag
     5221 taacttatag cccagcttct ggttgcacac caaatacttc ctcttatttt tatttagatg
     5281 gaacaagtaa ttcatctcaa aatccaaaat taaaatttga taatcctggc acatatacta
     5341 ttggtttata tattcaaaat ggagattgta cttcatctac agtttttaaa caaataactg
     5401 taaaaggcaa gccatcagtt tctccaataa atgcatccaa tgtttgccaa aatggttcaa
     5461 catcgccaac ggttactgtt ggtaattgta atacatctgg tatgccaact tatgcatgga
     5521 gttttcctgg tggaaatcca acttcatcta ctcaaccaac tccaggtaat gtaacttata
     5581 caacatttgg tacgccaaca atttctttag atgtaactaa cgaatgtggt acaacctatg
     5641 taattaaaac aatcacagtt tctgcagctc ctacagttgt tgttccaagc aataaaacat
     5701 tttgtgctgg cgatgcaaca ggaatgttga attttagtag tagtgtatca actgcaaatt
     5761 acacatggac aaacagtaat actgctatag gtttaaatgc tagtggctca gcttcttctt
     5821 caaatccatt tattaatagt tttatagctc aaaataatac tgcttcaccc attactgcaa
     5881 ctataacagt tacaccagtt agtggttgtt cgggaatacc acaaacattt accataactg
     5941 taaacccaaa gccaacattg cccgtagtag taagtccaat taattattgc ttaaatgaag
     6001 tagctactgc attaaatgca tcagctacag gtggtaacac tttaatgtgg tataataatg
     6061 cagcattatt aaatggtaca gttacagctt atactccatt aaccacttct acaggtacaa
     6121 caaactatta tgttacacaa gaaaatagtt atggctgtaa aagtagtgca gctactattt
     6181 ctgttactgt agttccgatt atttcaaata acacaatagg taataaccaa actatttgtg
     6241 caggagctac acccaataca ttaactcaaa catcaacaac aactggtggt acaggtgttt
     6301 atacttatca atggcaacaa agtacagacg gtggcttaac ctggagttcc atcataggtg
     6361 ccaatacttc ttcctattta cccattggca tttcagttaa tacacaattt agaagaattg
     6421 ttttaagtgc ttgttccgat acctccaatg ttattagtat aactattttg ggagtgttaa
     6481 ataatacagg agtttctgca gcacaagtaa tttgccaagg aaatactcca gctatgttag
     6541 atggtgatac gccaaatggt ggaaatggga catttaattt tacttgggaa caatctacaa
     6601 ataatacaac ttggagtatt atttctggaa ttactacaga agattatcaa ccacctgcac
     6661 ttaatgttac aacttattac agaaggaaaa cagaaagtgg cagttgtgca gcatactctg
     6721 catctataaa aattactgtt aatccaaaac cagttattac accaattgct aatgccactt
     6781 tgtgtaacag cataactact acaccaattg tttttactag cacacctaat actggtgtaa
     6841 cttatgtttg gcaaaatgat aatatagata ttggtttacc tttaacaggc agtggcaata
     6901 ttgctgctta ttctacaaca aataacagta gccctaaagt gcctatcata ggaaatatta
     6961 gcgttgttcc aacttataca ttttcgtctg ttgcatgttt aggagatact ggtaagttct
     7021 ctataactat tttgcctacc atttcaatta atgcagttgc agatactact atttgctctg
     7081 gctcatcatt gcctgctaaa acatttatac atgatgcagg tagttatgca gaaactaatg
     7141 taacatatca atatactatt tctggctctg gtacaagctt agtttctggt actggcagta
     7201 ctgtaccagc ttatactgca aataatgtag gaaatactaa tttaacaaca acggttacag
     7261 ttaaaccagt ttatactttt aatggtaaaa gctgcgatgg cattgcagaa acttacacta
     7321 ttaccattac accatctact ccaactgctg cagcaggtaa tgatactata ctttgttctg
     7381 ccaatactta taccatgcaa gcaacacttg caggtggtag tactggtgtt tggtcagagt
     7441 ttggaggaaa ttcaacaacc attcaaaata caacttctcc aacaacacaa ataacaggat
     7501 tacttcctaa taatacatac aaatatgttt ggaatgttac aggggttgca agctgcccag
     7561 gtacttcaga tactataact attataaatt atgcatctgt tgttaatcaa atagataata
     7621 ccaccaaagt tatttgtgct acccaaacta taaatattaa tggaaactca cctacaggtg
     7681 gcagtggtag ttacacttat caatggcaac aaagtacaga tggaggattg acctggacaa
     7741 acataccttt taaaactagt aaagacttat cttatatacc aactacttct gtattaataa
     7801 gaagaattgt ttattcgttg ccatgtattg aacaaagtag cacaacaatt gttacagtgc
     7861 aacctgctat tagtaacaat actatttctg caaaccaaag tatttgtatt aatactgttc
     7921 caacaacgtt gataggcact gttgcaacag gtggcgatgg aaacatttcg tatcaatggc
     7981 aactaagtac caatggaggt ataacatgga gtgatatttc tggagcaacc tcaaaagatt
     8041 tttctccatc ggcactaaca gttacaacaa aatatagaag agtagtaagc actagtttat
     8101 gttttggaac acagggtaat aatagtttgg caattacaat tacagttaat ccagattctc
     8161 atgcaaatta tacttttaca aacaacatag gctgtgcacc tttcttaatt aattcaataa
     8221 atattaaacc aactttattg ccattacaaa atggcaccta taactggtat gcaaataata
     8281 cgtatttagt tggtgggcct agttttcctg gttttacatt aacaaatcct ggcgatagca
     8341 ttcaaataaa attagtaact acaagtccgt ttggttgtaa ggcagatagt gtaacacata
     8401 aattttatac agttgcaaaa cctcaaacat catttacagc gagtgatact ataggttgtg
     8461 ggcctctaaa tgttttattt actaatacta cacccaatat aaattggttt aattatcaat
     8521 ggtcgtttgg caatggatta acttctaatt taccacaacc aaatacagta acttttgcaa
     8581 tcaacccaac tttttttgat actatttata ctgttcaact ttctgcaaca actgtatgcc
     8641 aaacagtaaa agatacacaa tatattaggg taaaatcaaa accaaaggca gtttttacac
     8701 ctaataaatc tgttggttgc tcaccaatga ctgtgttttt taacaacact tccaaaggta
     8761 ataatgtaca atataaatgg aactttggag atggaggagc cgacacttta gttactacta
     8821 aatcctcaat gtcttatact tattacagag gatttcaaga tactgttgat gttaggttaa
     8881 tagcttttaa tgattgtggt agtgatacgc agacatacaa aattgtaatt tctcccaacc
     8941 taattcaatt agattttgca gttaatggta atgaaactca aggttgtaaa cccaaatcgg
     9001 ttaaattcat taatgcatcc aatggggcaa ctaatttctt ctggagtttt ggagatggta
     9061 atacattaag tacaacaaaa aatatagata ctgttacgca tacttatact actgcaggta
     9121 catttattgc aacactaaaa gcaagtaata attgtagcga tacaactagc gttgaaacta
     9181 ttcaaatttt taatacccca attataaact ttacggcagt accatcttcc atttgtattg
     9241 gagatacaat tcactttaca aaccttacag atactgcatc tagttactta tggagcttta
     9301 atgataacag cacatcaaca cttgttaatc caaaacatat ataccctgta gctggcaatt
     9361 atttagtaca attaaaagca ttaaggcaat ataatagtgg cttaacttgt atagatagta
     9421 ttagtaaacc tatttctgta gtttcaaaat taaaaggttc ttttacagca aatgatacaa
     9481 ttagcccttg tgttcctttt acattatcaa tggtaaataa tacaccagca cttcaaacca
     9541 tttggtatag taataatata cctattgata caggcaatgt tgctacatac acttttaatc
     9601 aggtaggtag ttatttaata aaaatgaatg caactaatgc tgctggttgt ataaaagaag
     9661 atacgcaaac tgttgtagtt agaggcccat ctggaacttt tcaatatgat catggcatta
     9721 tctgctctaa caatgctgtt tattttgaag tattgccaag caataccgat agtattaagt
     9781 ttaattttgg cgatggtaat tttttaacca ccactgccaa tagtgtgtat catcaatata
     9841 atcaaacggg tagttttatt ccatcagtaa tattatattc taatttttca cctgcttgta
     9901 ctgttacatt acaaggcaca gatactatta aaattgatta tgtaaaagca gggtttaaat
     9961 attcatcaca aacctattgc gactcaacaa aaatattttt taatgatact tctaggcatg
    10021 ccttacctat tgttagttgg caatggaatt ttggcgatgg caattttagt actctacaac
    10081 atccaaccca tacttataaa aataccaaca attgggcggt gcagctaatt actaaaactg
    10141 ttggaggttg tacagataca gtgaattata atatgtttgt aaaagtaaac aacaaaccat
    10201 ctaatactat acttgcacct ttggtagcat gtgcattaaa gccaataaat tatcaggcac
    10261 aaacaacaag tgtagatagt ataatattta ataattggca atttaataat gggctaacaa
    10321 cacttggcaa caatgtaaat aatacctaca ttttaccagg tacttattat gctactttaa
    10381 tttctggtac agcttatggt tgtacagata cagctctaca taccattaca ataaatccta
    10441 ccccaactgt tgttacaaca tctaatcaaa taatttgttt aggtagttct attcaattaa
    10501 atgtatctgg tgcaaatact tatagctggg caccaattaa taataccctt agctgtttaa
    10561 catgtatcaa tcctattgct acacctatta catctaccat gtatattgta gaaggtacta
    10621 atacttttgg atgtagtaat aaagatagtg taatggtaac tgttgcacag cctataacaa
    10681 tacaaacatc tgccaatgat acaatatgta ttggtcagtc tgctcaactt agtgcaactg
    10741 gtgcaacaac ttatcaatgg atgccttcct caagcttgca aaataataat actgcaaacc
    10801 ctattgctac acctgccatt actacaactt accaagtaat tggattcgat aattacaatt
    10861 gctttcaaga tacaggcttt ataactgtag ctgtaggaaa atatccaacc ataacattgg
    10921 aggctgataa agtattgcca acaggtactc aattagcatt aaagagcact gtaactaatg
    10981 gccctattac taattggcta tggactccat cggcagactt aaattgtaac acttgtgctt
    11041 tgccaattgc aactattaaa aacgatgttt gttatactgt aacagctgcc aatatatatc
    11101 actgtgcagc tacagataca atttgtatta aaacattttg cgaaggaacc caagtattta
    11161 tacccaatgc ctttacacca gatggagatg gtataaatga tgtattaatg gtaagaggca
    11221 gcggaattaa aactgtgaaa agttttagaa tatttaatag atgggggcag gtagtttttg
    11281 aaaaatctaa cgttacacca aacgatcctg cacaaggtt
//
LOCUS       DAOQPK010000050        11113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_3030_length_11113_cov_4.298336, whole genome shotgun sequence.
ACCESSION   DAOQPK010000050 DAOQPK010000000
VERSION     DAOQPK010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11113
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3030_length_11113_cov_4.298336"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1246
                     /locus_tag="PLU36_03910"
     CDS             2..1246
                     /locus_tag="PLU36_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HMZ45926.1"
                     /translation="MMHPKHLSILDFSYPLSEERIAKFPLSLRDTSKLLVYNNEEISQ
                     DYYYNLHNHIPENSLIIFNNTKVVEARLLFEKTTGSVIEIFCLEPGESYGDITVAMMQ
                     KSKVYWKCLVGGAKKWKEDFLLKELNENGNNIQLQAKIIEKKNDYFLIEFCWNNNELC
                     FSELLHLFGVIPLPPYIKRSTTEQDRVTYQTIYAKFDGSVAAPTAGLHFTDVMVKNLK
                     AKNIQPIYTTLHVGAGTFKPVKALTMQEHEMHAEYIEVTRQFIEQLLSKDFPTIIAVG
                     TTSLRTIESLYWMGVKIKTQTNFTSFNEIAINQWDAYELPQEILVNDALNSLLQWMIE
                     NNKEKIITKTQIIIAPGYNLKVADALVTNFHQPQSTLLLLVAAIAGNNWKKIYHFALE
                     NDFRFLSYGDGSLIWKNKKTTD"
     gene            complement(1236..3905)
                     /locus_tag="PLU36_03915"
     CDS             complement(1236..3905)
                     /locus_tag="PLU36_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HMZ45927.1"
                     /translation="MSKLYKINKKILTVFIVSIALFFLTLSLEANVVINNIAHPPIQD
                     TIPSKVKIGKDSIQNKKTDSTKVLTKDTLVFSKDSIDAPVKYSATDSGVLIISTKQFI
                     LYGSATAKYQDMELEAATIEYLQDKQTIKAKGSSDTSKNSLHKTKFKQAEMSTQSDSI
                     AYNLKTQKGITYNTYYQEGELFVNAGKLKKADSNIVYAYKGSFTTCNLDTPHFSFRTK
                     KMKIINNKIGVSGPAHPEFEGVPIPIYIPFGIYPLNRGRKSGILTPTFTSSEDFGLGF
                     EGIGYYKVLNDNLDITTRANFYTYGGWSITANSKYLKRYRYAGNVNIAVQKNKTLNRY
                     ATKSEDEFTSSSSYMINWSHSRDSKARPGTNFTANLNFGSTRFNKNILNNPYQNFNNQ
                     LSSSISYSKNWNSKYNLSLNLNHNQNNKTHLVNLSLPNANFNVVTFYPFEKKEAIGTA
                     KWYEKISIGYSGNLQNRISFYDTAFSSYKLLDTLQWGAEHNIPIGLALPALGPINIAP
                     SISFQQRWFGQKTHRYWSMADSLVKTSTNKGFYLQNSMSFGISASTRIFGTYQFKKGK
                     VKAIRHEIRPSFGINYTPDLVSKNFYNVQVDTSGRTMRFSEFDGGIYSSYNEGNFGGI
                     TFGIDNLLEMKVRDKTDTSKNADRKIKLIDGFGFSSSYNLLADSFALGSFNLYVRSTL
                     FDKINITASASLDPYQVNKFGQRVNAFMLKKGKLGRITNGSLAISTNFQSKSKDGKNE
                     EDSKNGIPNDPFLTPDEQQRQLDYIRQNPAEYTDFNIPWKVDLSYSLSFYQAIKPDYS
                     GYNTETFSSLNFNGDFSLSPKWKVGATGYYDLKYMNFQQFSMFISREMHCWQLSVNIT
                     PVGLWRSFNIVISPKSGILRDLKINRNRTFSNL"
     gene            3976..4923
                     /locus_tag="PLU36_03920"
     CDS             3976..4923
                     /locus_tag="PLU36_03920"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HMZ45928.1"
                     /translation="MTKRISFSLLILLTLLATCIFSFKSKDAQQPIQKQKLRKIVIDA
                     GHGGRDIGAEGKYSNEKTISLDVALKLQKTIEQEIPDVEVYMTRTSDFYDSPITKADK
                     ANAAKGDLFISIHCNSASGNRHRELVGYKTVTSKNRKTKKTTTRKVPQYRYYTTPSVA
                     KGTETYIWAIRKSDQKTNAVKSHEDAYLDSATAKELGDFNPDDPEMEILLNLKTKQYF
                     DRSARLAITIEEEFIKTGRPSRQAQQRGKGIWVLQAVAMPAVLVEIGFISNAEEEDYL
                     NSQAGQQEITQALTKAIKRYKYTLETTGIRPNGTNSQKK"
     gene            5105..7015
                     /gene="dnaK"
                     /locus_tag="PLU36_03925"
     CDS             5105..7015
                     /gene="dnaK"
                     /locus_tag="PLU36_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HMZ45929.1"
                     /translation="MGKIIGIDLGTTNSCVSVMEGNEPVVIANEEGRRTTPSVVGFLK
                     NGERKVGDPAKRQAITNPINTIASVKRFMGRKFDEVTEEISHWSYKIVKGDNNTIRVD
                     IDGRLYTPQEISAMVLQKMKKTAEDYLGTEVNEAVITVPAYFNDAQRQATKEAGEIAG
                     LTVKRIVNEPTAAALAYGLDKKGQDQKIAVFDLGGGTFDISILDLGDGVFEVKSTNGD
                     THLGGDDFDKVIMDWLADEFKADEAIDLRKDPMALQRLKEASEKAKIELSSSSETEIN
                     LPYITAVDGVPKHLVKKLSRAKFEQLSDSLITRCLKPCEVALKDAGYSASQIDEIILV
                     GGSTRIPKVQEVIEKFFGKKPNKGVNPDEVVAVGAAIQGGVLTGEVKDVLLLDVTPLT
                     LGIETMGGVLTPMIPANTTIPTKKMEIFSTASDNQPGVQIHVLQGERPMAAQNKSLGI
                     FNLDGIPPAPRGVPQIEVTFDIDANGLLHVSAKDKGTGKEQKIHIEAGSGLSKEEIEK
                     MKAEAKAHESEDKEAREKVEKINQADSLIFQTEKQLKEFGDKIPADKKTPIETALTKL
                     KEAHKAQDIAAIDTALTEMNTAWTAASEEIYKAQQQATPDAGATTQDATNTQSNSTAN
                     DTVEDAQFEEVK"
     gene            7052..7276
                     /locus_tag="PLU36_03930"
     CDS             7052..7276
                     /locus_tag="PLU36_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45930.1"
                     /translation="MNEIQGLLLNVDAPELMSGPFHDSLPLSYACDYGMIFIRSKDGI
                     SHNPLEYSSYEDIALGTQVLLGTVQRILDI"
     gene            complement(8100..8678)
                     /locus_tag="PLU36_03935"
     CDS             complement(8100..8678)
                     /locus_tag="PLU36_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45931.1"
                     /translation="MRYLVTVTFLFFFIKSYNQEDYTKIIEPNIFRTYIAAPVNILGV
                     HYEHKLKQNLSIAGKIGLNSAMGISSGLSEGRVRIQTSLLSSVEARYYFNIKRREERN
                     KTIQNFSGWYLGLEPFVRTHSLFAINEAKKDNNGLTGVFVNLGFQRQPPNRKIYGGFY
                     IGYSPVVNSMSSTLNSSEIDRVWLNFLFGFVL"
     gene            complement(8692..9810)
                     /locus_tag="PLU36_03940"
     CDS             complement(8692..9810)
                     /locus_tag="PLU36_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45932.1"
                     /translation="MKYFVAEYEIDAAILNEDLQIIIDDTLFQVTRIGVFKIVNDKID
                     NFFELYEEHEDEILYDPNITPLPGEIPLPDGSYRVENGIDRVPAPVNILYDDSDPGIT
                     SPGGGYVPPCSITLPNDFQYATYDVDSDFKRNEVLYVESNTRRFKFETFKTNFLSLGV
                     LRTVGIRGKLQRQRKFLGIKYWGESYADEIILNVENMDLHTDYVIPTPQVYNTLPAPQ
                     FNGLFNLQIGNHILEEVLDIRVNVNLPFNQNISKATVSNYLNGTLNSILNNQFHNYFS
                     PFVNSLIANIDPSYPTRYANHAKRVSALKEANKVRYTLAEGAKSQGYSHVNVWRFDWN
                     IGTPWGNGTTYSYEMKAGSFSGRARVGCKWYGIRIVRL"
     gene            complement(9841..10146)
                     /locus_tag="PLU36_03945"
     CDS             complement(9841..10146)
                     /locus_tag="PLU36_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45933.1"
                     /translation="MRNKIFISLIAILLLCACKKEQPSITNEIDIKIPMIRKDGKFKN
                     GVLVFDSREHLIKTYRALENNSDNANNIIPSSFNSLQKYFNKFRNSQYQNRTADDNI"
     gene            10219..10413
                     /locus_tag="PLU36_03950"
     CDS             10219..10413
                     /locus_tag="PLU36_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45934.1"
                     /translation="MILVLEHSNYYKEYLNTYASLIVNQNAGLPVKLLMRNAEGYFKE
                     LKFEEQGTGKNKKYIIKICK"
     gene            10420..10926
                     /locus_tag="PLU36_03955"
     CDS             10420..10926
                     /locus_tag="PLU36_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45935.1"
                     /translation="MKKLMIILGIMVLLGIKLNAQSPVTDTLQWLKTNIEQRKNLFIN
                     KPLSVLLDSLKELKQGIVEYTGPDWYAIGNLNDTIWTTRINLYFEEMTGSAKEEMHHN
                     VFYSNNFKDTLNTHIKILRIKFTQPVIFLRKWWSYNSDTLGSNEWLPKVAALYKNAIV
                     KDVKVDEY"
BASE COUNT         3944 a         1706 c         1764 g         3699 t
ORIGIN      
        1 aataatgcac cccaagcatt tatctattct tgatttttct tatccacttt ctgaagaaag
       61 aatagcaaaa tttcctttat ctctaagaga tacaagcaaa ctacttgtgt ataacaatga
      121 agaaatttca caagactatt attataattt acacaatcat attcctgaaa actctttaat
      181 tatttttaac aacactaaag ttgttgaagc aagattattg tttgaaaaaa caactggcag
      241 tgtaattgaa attttttgtt tagaaccagg tgagagttat ggggatatta ctgttgccat
      301 gatgcaaaaa agtaaagtat attggaaatg tttagttggt ggagccaaaa aatggaaaga
      361 agatttttta ctaaaagaac tgaatgaaaa tggaaataat attcaactac aagcaaaaat
      421 aattgaaaag aaaaatgatt attttttgat tgaattttgt tggaacaata atgagctatg
      481 cttttctgaa cttttacatt tgtttggcgt aattccttta ccaccttata taaaaagaag
      541 cactacagaa caagatagag ttacctatca aactatttat gcaaaatttg atggaagtgt
      601 tgcagcccca actgctggtt tgcattttac ggatgtaatg gttaagaacc taaaggcaaa
      661 aaatattcaa cctatttata ctacattaca tgtaggtgca ggtactttta aacctgtaaa
      721 ggctttaaca atgcaagagc atgaaatgca tgcagaatat attgaagtta ccaggcagtt
      781 tattgaacaa ttattaagta aagattttcc caccattata gcagttggta caacatcttt
      841 aagaacaatt gaaagccttt attggatggg tgtgaaaata aaaacacaaa ccaattttac
      901 atcatttaat gaaattgcca ttaatcaatg ggatgcttat gagttacctc aagaaatttt
      961 ggtaaacgat gccttaaata gtttattaca atggatgatt gagaataata aagaaaagat
     1021 tattactaaa acacaaataa ttattgcacc aggttacaat ttaaaagttg cagatgcttt
     1081 agttactaat tttcatcagc cacaatctac tttgttattg ttggtagctg caatagcagg
     1141 aaacaattgg aaaaaaattt accactttgc attagaaaat gattttagat ttttaagcta
     1201 tggagatggt agtttgattt ggaaaaacaa aaaaactaca gattagagaa tgttctgttt
     1261 ctatttattt ttaaatctct tagaatacca cttttaggac ttataacaat attaaaacta
     1321 cgccacaaac caacaggggt aatatttaca gaaagttgcc agcagtgcat ttctctagaa
     1381 ataaacatgc taaattgttg aaaattcatg tattttaaat cgtaatatcc tgttgcacct
     1441 actttccatt tggggcttaa actaaaatct ccattaaaat ttaagctact aaaagtttct
     1501 gtattgtagc cactataatc tggttttata gcttgataaa atgaaagaga gtaagataaa
     1561 tctaccttcc atggtatatt aaaatctgta tattctgcag ggttttggcg aatgtaatct
     1621 agctgtcttt gctgttcatc tggtgttaaa aatgggtcat tgggtattcc atttttacta
     1681 tcttcttcat ttttgccatc tttagatttg ctttgaaagt tagtactaat agcaaggctt
     1741 ccattagtta ttcttcctaa ttttcctttc tttagcataa atgcatttac cctttgccca
     1801 aatttattta cttgataagg atcaagactg gcacttgcag taatatttat tttatcaaac
     1861 aatgtacttc ttacatataa attaaaacta cctaatgcaa aactatctgc tagtaaattg
     1921 tacgaagagc taaagccaaa cccatcaatt aattttattt ttctgtccgc atttttgctt
     1981 gtatcagttt tgtctcttac tttcatttca agtaagttat caataccaaa agtaatacct
     2041 ccaaaatttc cttcattgta agagctataa attccaccat caaactcaga aaaacgcatg
     2101 gttctaccag aggtgtctac ttgtacattg taaaaatttt tgcttactaa atctggtgta
     2161 taattaatac caaatgatgg tcttatttca tgcctaattg cttttacctt acctttttta
     2221 aattggtaag ttccaaaaat tctggtgctg gcactaatgc caaagctcat actattttgt
     2281 aggtaaaagc ctttgtttgt acttgtttta actaaagagt ctgccatgct ccaataccta
     2341 tgtgtttttt gcccaaacca tctttgttga aaagaaatgc ttggtgcaat attaataggg
     2401 cctaaagcag gtaaagctaa accaattggt atattatgtt ctgcccccca ttgcaaagta
     2461 tctagcaact tgtaggagct aaaggcagta tcgtaaaatg aaattctgtt ttgtaaattt
     2521 ccagaatatc caatactaat tttttcatac catttagcag tgccaatggc ttcttttttt
     2581 tcaaaagggt aaaaagtaac tacattaaaa ttggcatttg gtaaacttaa atttactaaa
     2641 tgtgttttgt tattttggtt atgattaagg tttaaagata aattgtattt actgttccag
     2701 tttttactat atgaaataga agatgataat tggttgttga aattttgata agggttattc
     2761 aaaatatttt tgttaaacct tgtactacca aaatttaagt tagctgtaaa atttgtacct
     2821 ggccttgctt tactatctct gctatggctc caattaatca tgtatgaaga agagctagta
     2881 aattcatctt ccgattttgt agcataacga tttagcgttt tatttttttg cacagcaata
     2941 tttacattgc cagcatatcg gtatcttttt aaatatttac tatttgcagt tatactccat
     3001 ccaccataag tataaaaatt agcccttgtt gtaatatcta aattatcatt caaaactttg
     3061 taatagccaa taccttcaaa gcctaatcca aaatcttcgc tactggtaaa agttggggtt
     3121 aaaatacccg attttctacc acgatttaaa ggataaattc caaaaggaat ataaatagga
     3181 attggtacac cttcaaactc aggatgtgca ggcccactaa caccaatttt attattaatt
     3241 attttcattt ttttagttct aaaactaaaa tgtggtgtat ctaaattaca ggttgtaaaa
     3301 ctacctttgt aagcataaac aatattacta tctgcttttt ttaatttccc tgcattaaca
     3361 aataattctc cctcttgata ataagtatta taagtaatac ctttttgcgt ttttaagtta
     3421 tatgcaatag aatcgctttg tgtactcatt tctgcttgct taaactttgt tttatgcaaa
     3481 gagtttttag atgtatcaga agaacctttt gctttaatag tttgcttatc ctgcaagtat
     3541 tctattgttg cagcctcaag ctccatatcc tgatatttgg cagttgcaga accatataaa
     3601 ataaactgtt tggtagatat aattaaaact ccagaatctg ttgcactata ttttactgga
     3661 gcatctatag agtctttgga gaaaactaaa gtatcttttg ttaaaacttt agtgctatct
     3721 gtttttttat tttgtataga atctttgcca attttgactt tagaagggat tgtatcctga
     3781 ataggaggat gagcaatatt attaatcact acattggctt ctaatgataa tgttaaaaaa
     3841 aacaaagcaa tacttacaat gaaaacagta agtatttttt tattaatttt gtacaacttg
     3901 ctcatagtat aactgagcaa aaataagagc taatacttta aacatcatac aaacacttgt
     3961 atttaactat ttgatatgac taaacggatt tcattttcat tactaatttt attaacttta
     4021 ttggcaacct gtatattttc ttttaaatct aaagatgcac aacagccaat acaaaaacaa
     4081 aagctaagaa aaatagtaat tgatgcaggt catggtggaa gagatattgg tgcagaaggc
     4141 aaatattcca atgaaaaaac aatttcgtta gacgtggctt taaaattaca aaaaaccata
     4201 gaacaagaaa taccagatgt agaagtgtac atgactcgta cttcagattt ttatgattct
     4261 ccaattacca aagccgataa agccaatgct gcaaaaggtg atttatttat ttctattcat
     4321 tgcaactctg caagtggcaa caggcataga gaattagtag gatataaaac cgtaacaagt
     4381 aaaaatagaa aaacaaaaaa aactactact cgtaaagttc ctcaatatag atattacact
     4441 acaccaagcg ttgcaaaagg taccgaaact tacatttggg caatacgtaa atccgatcaa
     4501 aaaacaaatg cagttaaaag ccacgaagat gcatatttag atagtgcaac agcaaaagaa
     4561 ttaggagatt ttaatccaga tgatcctgaa atggaaatat tactaaactt aaaaacaaag
     4621 caatattttg atagaagtgc aaggttggca ataacaattg aggaagaatt tattaaaaca
     4681 ggccgtccta gccgtcaggc tcaacaaaga ggcaaaggaa tatgggtgtt acaggctgta
     4741 gcaatgccag cagtgttagt agaaattggc tttatcagta atgcggaaga agaagattat
     4801 ttaaatagcc aggctggtca gcaagaaatt actcaagcat taacaaaagc aattaaacgt
     4861 tataaataca cattagagac tactggaatt aggcctaatg gaaccaattc tcagaaaaag
     4921 taaaatattt tatctctctt ttctcttctt tttttcataa tctaatgaaa atttttctta
     4981 ttttcttaac ccgttctgcc ataaatattt aatacaaatt atatctattt attttactgc
     5041 cattttttca taaataatga aatggcataa aggttgaaca atgtaaagca ttaataaatt
     5101 tactatggga aaaataatag gtatagattt aggcacaacg aatagttgtg taagtgtaat
     5161 ggaaggcaac gagccagttg taattgccaa tgaagagggc agaagaacaa caccatctgt
     5221 tgttggtttt ttaaagaatg gagaacgtaa agtaggagac ccggcaaaac gccaggcaat
     5281 tactaacccc attaatacaa tagctagtgt taaaagattt atgggaagaa agtttgacga
     5341 agtaacagaa gaaattagcc attggagtta caaaattgta aaaggagata acaacaccat
     5401 tcgtgttgat attgatggaa ggttatatac acctcaagaa atttcggcaa tggtacttca
     5461 aaaaatgaaa aaaactgccg aagattattt aggaacagaa gtgaatgaag ccgtaattac
     5521 tgtacctgct tactttaacg atgcacaaag gcaagctaca aaagaagctg gtgaaattgc
     5581 tggtttaact gtaaaaagaa ttgtaaacga accaacagca gcagctttgg cttatggttt
     5641 agataaaaaa ggccaagatc aaaaaattgc tgtatttgat ttaggtggtg gaacttttga
     5701 tatttctata ttagatttag gcgatggcgt ttttgaagta aaaagcacta atggagatac
     5761 ccatttaggt ggtgatgatt ttgataaagt aataatggac tggttggcag atgagtttaa
     5821 agctgatgaa gcaatagatt taagaaaaga cccaatggct ttacaacgct taaaagaagc
     5881 ctctgaaaaa gccaaaatag aattatcgtc ctctagcgaa acagaaataa acttaccata
     5941 tataaccgct gttgatggtg ttccgaaaca cttggtaaaa aaactttcaa gagcaaaatt
     6001 tgagcaatta agtgatagtt taattacacg ttgtttaaaa ccttgtgagg ttgcattaaa
     6061 agatgctggt tattctgcca gccaaataga tgaaattatt ttagtaggcg gtagtactcg
     6121 tattcctaaa gtgcaggaag tgatagagaa attttttggt aaaaaaccaa ataaaggtgt
     6181 aaacccagat gaagttgttg cagttggtgc tgccatacaa ggtggtgtgt taactggtga
     6241 agttaaagat gttttattgt tagatgttac acctttaaca ctaggtattg aaacaatggg
     6301 tggtgtgtta acaccaatga ttcctgctaa tactacaata cctaccaaaa aaatggaaat
     6361 atttagcaca gcaagcgata atcagcctgg tgttcaaata catgttttac aaggcgaaag
     6421 gccaatggct gctcaaaata aaagtttagg tatttttaat ttagatggaa taccaccagc
     6481 accaagaggt gtaccacaaa ttgaagtaac ttttgatata gatgccaatg gtttattaca
     6541 tgttagtgca aaagataaag gaactggtaa agaacaaaaa attcatattg aagctggtag
     6601 tggtttaagt aaagaagaga tagaaaaaat gaaagcagag gctaaagccc atgaatcaga
     6661 agataaagaa gcaagagaaa aagtagagaa aattaaccag gcagatagtt taatttttca
     6721 aacagaaaaa caattaaaag aatttggaga caaaattcct gccgataaaa aaacacctat
     6781 tgaaactgca ttaactaaat taaaagaagc tcataaagca caggatattg cagctataga
     6841 cactgcttta acagaaatga atacagcctg gactgcagct agtgaagaaa tttacaaagc
     6901 tcagcaacaa gcaacaccag atgcaggtgc aacaacacaa gatgctacaa acacacagag
     6961 caacagtaca gcaaatgata cagtagaaga tgcacagttt gaagaagtga aataataatt
     7021 tatccgaata gaattaatca taataaagcc cttgaatgaa attcaggggc ttttattaaa
     7081 tgtagatgca cccgaactaa tgagtggccc ttttcacgac tctttaccac tatcctatgc
     7141 ttgtgattat ggaatgattt ttataagaag taaagatggc ataagccaca atcctttaga
     7201 atattcttct tacgaagata ttgctttagg tacacaggtt ttattaggaa cagtgcagag
     7261 aatacttgat atttaagaag catttttaaa gaaacatact taatatcaac cttagcctct
     7321 acagtttgtc atcctgagcg aagcgaagga tctttaattc tatgtcattc cgagtttgcg
     7381 aggaatctct attcttaact ttacagatgc ttcgttgcct cagcatgaca gtaagtgtac
     7441 attgtcattc agaatgacaa accttttttg ttttacttta ttcaatatgt catcctgagc
     7501 gaagcgaagg atctccttat gttttattcc aaagcacttg cagattcttc atttcattca
     7561 gaatgacaaa ccttttttgt tttactttat tcaatatgtc atcctgagcg aagcgaagga
     7621 tctactgatg gtttgtacca aagcacaaac agatgtttcg tttcactcaa catgacattg
     7681 ttgtttttgt ttcatctcat tcggaatgac ataaatctaa accgatatat tatttaaaca
     7741 tattagattt acaacaattt cttactaatt ttccaccaga tagtgtatta aatggtaatg
     7801 aatggagtaa tgattataag ttatcaaata tagacggtgg tagtttatat gatgcttggg
     7861 aggatttatg tcgtgaatta attatttccc ataattatcc taaggaaaaa gcacaaactt
     7921 atgcaaactt aatattgatg aagtattttt ttaacacagg agtagattta tatataaaag
     7981 gtgatgatgg ggcaatgcat ctgtatattc tttatccttc atcttttatc acgcatcatt
     8041 ctactaacta aataccatcg aataaataag ggttggttca atatgaatta cattatcctt
     8101 tataaaacaa atccaaataa aaaattaagc caaaccctgt caatttctga actgtttagt
     8161 gtactgctca tgctgtttac aacaggtgaa tagcctatat aaaaaccccc ataaattttt
     8221 ctattagggg gctgtctttg aaatcctaag tttacaaaaa cacctgtaag tccattatta
     8281 tcttttttag cttcattgat agcaaacaaa ctgtgtgttc ttacaaaagg ctcaagacct
     8341 aaataccaac ctgaaaaatt ttgaatagtt ttattacgtt cctctctgcg ttttatatta
     8401 aaataatacc ttgcttcaac agatgaaagc aaactggttt gtattcttac tctgccttca
     8461 cttaatcctg aacttatgcc cattgcagag tttaagccaa ttttacctgc tatgctaaga
     8521 ttttgtttta atttatgttc ataatgtact cctaagatat ttacaggagc agctatataa
     8581 gttctaaaaa tattgggttc aattatttta gtgtaatcct cctgattgta tgacttaata
     8641 aaaaaaaaca aaaaagtaac tgtaactaag tatctcataa agctattttt attataacct
     8701 aacaattcta attccatacc acttacagcc aactcttgct ctaccactaa aagaacctgc
     8761 cttcatttca tagctataag tagtaccatt tccccaagga gtacctatat tccaatcaaa
     8821 tctccaaaca tttacatgag aatatccttg acttttagct ccctcggcta atgtatatct
     8881 tactttattg gcttccttta aagcactaac tctcttagca tgattggcat atcttgtagg
     8941 gtagcttggg tctatattag caatcaagga atttacaaat ggtgagaaat aattatgaaa
     9001 ttggttattc aaaatactat tcaaagtgcc gtttagataa ttgctaactg tagctttaga
     9061 tatattttga ttaaaaggca aattaacatt aacgcgtata tccaaaactt cttctaaaat
     9121 atgatttcca atttgtaaat tgaaaagccc attaaattga ggtgcaggta aggtattata
     9181 tacttgtgga gttggtatta catagtctgt atgtaaatcc atattttcaa catttaaaat
     9241 aatttcatct gcatatgatt caccccaata tttaatgcct aaaaactttc tctgtctttg
     9301 tagtttccct ctaataccta cagttctcaa tacaccaagt gataaaaagt tagttttaaa
     9361 tgtttcaaac ttaaaccttc ttgtattaga ttctacatat aatacttcat ttctcttaaa
     9421 atcagaatct acatcatatg tagcatattg aaaatcatta ggcagagtaa tagaacatgg
     9481 tggcacataa cctccccctg ggctagttat acctgggtct gaatcatcat ataaaatgtt
     9541 tactggggct ggtactctat caattccatt ttctactctg tatgaaccat ctggcaatgg
     9601 tatttcccct ggcaagggtg taatattggg gtcatataat atttcatctt catgctcttc
     9661 atataattca aagaagttat ctattttatc gtttacaatt ttaaatactc ctattcgtgt
     9721 aacttgaaac aatgtatcat ctattattat ttgtaaatct tcgtttaata ttgctgcatc
     9781 tatctcatat tctgctacaa aatatttcat atcgtatatg tattgagcgg tatctacttg
     9841 ctagatattg tcatctgctg ttctattttg gtattgacta ttacgaaact tgttgaaata
     9901 tttttgtaag ctattaaaac ttgaagggat aatattatta gcattatcag agttattttc
     9961 taatgcccta taagttttaa ttaaatgttc ccttgaatca aaaactagaa cgccattttt
    10021 aaattttcca tctttgcgta tcatcggaat tttaatatca atctcattag ttatggatgg
    10081 ctgttctttt ttgcaagcac ataataagag aatagcaatc aaagaaataa aaatcttatt
    10141 tctcataaat atttttttgc aagagtaata tttttttatt aaataattaa tttatcgtct
    10201 atagtttctt ttaaaaaaat gattttagtt ttggaacatt caaattatta taaagaatac
    10261 ttaaacactt atgcttcatt aattgtaaat caaaatgcag gtttacctgt taagttatta
    10321 atgagaaatg cagaagggta ttttaaagag cttaaatttg aagaacaggg aacagggaaa
    10381 aataaaaaat acattattaa aatttgcaaa taatgctata tgaaaaagtt aatgataata
    10441 ttaggaataa tggttttatt gggtataaaa ttaaacgccc aatcacctgt tacagatacc
    10501 ctgcaatggc tgaaaaccaa tatagagcaa agaaaaaatt tgtttatcaa taaaccccta
    10561 agcgtattat tggatagttt aaaagaatta aaacagggaa tagtagaata tactggtcca
    10621 gattggtatg caattgggaa cttaaatgat actatttgga ctacaagaat aaatctttat
    10681 tttgaggaaa tgacaggtag tgcaaaagaa gaaatgcatc ataacgtttt ttattctaat
    10741 aattttaaag atacattgaa tactcatatt aaaatactaa gaataaagtt tactcagcct
    10801 gtaatttttc ttagaaaatg gtggtcttat aacagcgata ctttgggttc taatgaatgg
    10861 ttaccaaaag tagcagcctt gtataaaaat gccattgtaa aagatgtaaa agtggatgaa
    10921 tattaatgaa aaagatagtg tttacctttt ttagttttct acaccaacaa aaaataaaac
    10981 catgtcatcc tgagcgaagc gaaggatcta ctaatagttt gtgcaaaagc acttgcagat
    11041 ccttcatttc attcagaatg acaaaccttt tttgtttcaa tttattaaat catttcatcc
    11101 tgagtactca tag
//
LOCUS       DAOQPK010000051        11074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_3045_length_11074_cov_4.575188, whole genome shotgun sequence.
ACCESSION   DAOQPK010000051 DAOQPK010000000
VERSION     DAOQPK010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11074
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3045_length_11074_cov_4.575188"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1769)
                     /locus_tag="PLU36_03960"
     CDS             complement(<1..1769)
                     /locus_tag="PLU36_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HMZ45936.1"
                     /translation="MNKATTISKENASTHKSLQDIYIHGARMHNLKNVSVTIPRNKLI
                     VVTGVSGSGKSSLTIDTLFAEGQRRYAESLSSYARQFMERMAKPDVDYINGLCPAIAI
                     EQKVITRTPRSTVGSMTEMYDYLKLLYANIGKTVSPISNDIVKKQDVADVLNVIQQLN
                     KGTKVFIIVPFKQHAKRNTREELNILLQKGFTRVAMNNNSTTTEPEILKIEALLELDN
                     KTFKEKTASNFQVLIDRIVCEEFEEEDIHRLTDSISTAFYEGDGIVNVVTNTNKNYQF
                     NNRFELDGMRFEEPSQNLFSYNNSYGACTVCEGFGQTLDIDENLVIPDKMLSVFDGAV
                     APWKGEKMIWWKEQFIKNAVGFPIHKPVVDLTNEQYEQLWKGFGKALGIDDFFKEVEA
                     NLYKVHYRVMLSRYRGRTKCRHCKGYRLRPEALYIKINHMHIGELCEMQAKDLKVWFN
                     DLELSANESKVAQRVLLELHQRLQTLIDVGLGYLTLNRLANSLSGGESQRIQLTRSLG
                     SNLTDSLYILDEPSIGLHNRDTEKLIKVLQHLKKLGNTVVVVEHDEMMMREADYIIDM
                     GPLAAHLGGEVVAHALRQEVQQI"
     gene            complement(2000..5344)
                     /locus_tag="PLU36_03965"
     CDS             complement(2000..5344)
                     /locus_tag="PLU36_03965"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HMZ45937.1"
                     /translation="MANNKKTKRLQFSRKFTQDGISPYDLFEYDYRTSVIKNPNGEVV
                     FEMNNVEVPKQWSQIATDILAQKYFRKAGVPQQDGTLGRETSVKQVAHRMANCWKVWG
                     ERYDYFETSEDAQIFYEELVYSILNQSCVPNSPQWFNTGLHESYGITGKPQGHYYVDS
                     VDGQLKKSTNAYERPQPHACFILSVNDDLVNDGGIMDLWIREARIFKYGSGVGTNFSQ
                     IRGSGEKLSGGGTSSGLMSFLKIGDRSAGAIKSGGTTRRAAKMVCLDLDHPEVMEFIN
                     WKVEEEKKVAALVAAGYDNSYEGEAYSTVAGQNSNNSLRIPNEFFKVLEEGGDWELKA
                     RTDGRVMKKIPAKEVWNQIAYAAWRCADPGTQYDTTINEWHTCPQGGRINASNPCSEY
                     MFLDNTACNLASINLRKFFDEQTNKIDIEGVEYISRLWTVVLEISVLMAQFPSKEVAQ
                     LSYDYRTLGLGFANLGTILMLSGIAYDSEPARAIAGSLSAVMTGVTYKTSAEMASFLG
                     TFPRYEENRVDMLRVMRNHRAAAYDANEAYEGLSVKPIGINAKYCPDDLLKAATKAWD
                     DAVQMGEKYGYRNAQTTVIAPTGTIGLVMDCDTTGVEPDFALVKFKKLSGGGYFKIIN
                     GAVPNALKNLGYTQNQIDAIEKYAVGAASFEGAPFINPQILIAKGFTTDEVNKLNTAA
                     KSAFEIGFVFNKFILGEACLQRLGFTEEQYNDWSFSLLEALGFSDEEIDAANDYVCGT
                     MTVEGAPYLKEEHLPVFDCANKCGKKGVRYIHAHGHIKMMAACQPFLSGAISKTINLP
                     HEATVEEIAECYKLSWELGLKANALYRDGSKLSQPLSTKSDKKKKVDTTTETTQEDNK
                     EELMVNEASNIVDLSKLSVEDLLEEVTKRMNASTDTTLKRQLSRIVERKSLPAKRRGF
                     TQKAKINGQVIFLRTGEYGDGTLGEIFIDLAKEGSTVRSLMNCFAIAVSVGLQFGVPL
                     EEFVEKFVFTRFEPAGMVDHPNIKTTTSLVDFVFRALAYEYLGRTDLVHVLDKPEIGN
                     LGNDDEFNEQQELSSVRITASPKNHKHQVTSTATVKAEAGIDAVNAAAKNMQSDAPAC
                     NVCMHITVRSGTCYKCLNCGNSMGCS"
     gene            5761..6516
                     /locus_tag="PLU36_03970"
     CDS             5761..6516
                     /locus_tag="PLU36_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HMZ45938.1"
                     /translation="MKQDLYNTFRKNKLQRKKSFTVLIDPDKVNEQSLDKLVMLAVDA
                     KVDYFFVGGSLVISNHLDDCIKQLKAKCSIPVVLFPGSPSQVSKYADALLYLSLISGR
                     NPELLIGQHVVSAPFVKKSGLEIMPTGYMVIDGGAPTTVSYISNATPIPSDKNDIAVC
                     TAMAGEMLGMKLIYMDAGSGAKRPITESMIEKVASNIESPLIIGGGIIDAEKAYRNCS
                     AGADVIVVGNAIEKDTSLIKEIAAAVHSNSVTI"
     gene            6625..6831
                     /locus_tag="PLU36_03975"
     CDS             6625..6831
                     /locus_tag="PLU36_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45939.1"
                     /translation="MTQFILNIPTEKVQQFLITMTATGFANTIKNVLKEKNYNSFNLL
                     PLNNDLRKQHPYFDSDFFANDVFY"
     gene            6908..8359
                     /locus_tag="PLU36_03980"
     CDS             6908..8359
                     /locus_tag="PLU36_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HMZ45940.1"
                     /translation="MDIQCTEQELCILKKITNATSQLNTKSYLIGGFVRDKIIGRPTK
                     DADIVCLNNGIELAHLTATFFKPTPNVAYFKTFGTAQLKINLHTNSSKNNSTSFEIEF
                     ASARKESYTIESRKPKVATGTLEDDQNRRDFTINALAISLNTEDFGKLIDPFNGLLHI
                     EQGIIKTPLEPSKTFSDDPLRMMRAIRFATQLNFTIVPEAFEAIIANANRIKIVSQER
                     ITDELNKIMLSKKPSIGWHLLSKSGLLEIIFPQMMALHGTEYVDNKGHKDNFYHTLQV
                     LDNICEHTQDLWLRWAALLHDIGKPATKKFEDGLGWTFHSHEIVGAKMVPKIFNQLKL
                     PLNEKMRFVKKLVALHLRPISLTKENITDSAIRRLLYDAGEDIDALMLLCNADITSKN
                     KQKVEKYLNNFKLVEERMKEVEAKDNIRNWQPPISGEHIMQTFALTPSREVGLIKDAI
                     KEAILDGKIENNYAAAYQFMLLKASELNLHPKQ"
     gene            8427..8993
                     /locus_tag="PLU36_03985"
     CDS             8427..8993
                     /locus_tag="PLU36_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45941.1"
                     /translation="MAVLKFRIYLEEDDAVYRDIAIKHTQTFELLHFTILKAFEFDSK
                     HAATFYRSNDNWQRGREITFEKYDKEYAAPPLFMQEVPIGNEIKNTNQKFIYEYDFTK
                     GWIFLVELISVNKEEKTKEEYPLITRKEGLGPQQYGTKSLLGDKFSDIEERYDLTETA
                     EGFGNEGDSEDSENEMNEDAMDNDNEEL"
     gene            9047..9955
                     /gene="miaA"
                     /locus_tag="PLU36_03990"
     CDS             9047..9955
                     /gene="miaA"
                     /locus_tag="PLU36_03990"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HMZ45942.1"
                     /translation="MQLKNTVIIICGPTAIGKTKLAIEIALHLQTKIISADSRQCFKE
                     LNIGVAKPSLYELNLVEHFFINSNSIKEDTNAVSFETYALKKVNAIFQSTNYAVMVGG
                     TGLYIKAFCEGLDEIPAIPTELRNDIYTNYKANGLTWLQKQLQAKDKNFWEIAEQQNP
                     QRLMRALEVVEFTGKSINEFRKRKQQSRDFNIIKIGLETEREKLYSNINNRVDEMIQM
                     GLVEEVKNLLPFANYNALQTVGYKEIFDYLNGNCSLDFAIESIKQHTRNYAKRQITWF
                     KKDKDIKWFNQNEYNEIIAYINAVKP"
     gene            complement(9945..>11074)
                     /locus_tag="PLU36_03995"
     CDS             complement(9945..>11074)
                     /locus_tag="PLU36_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c assembly protein"
                     /protein_id="HMZ45943.1"
                     /translation="GLLTGAVQFLKYKNTPKSFIGKKFWLPVFISLIVALLIIFVGKV
                     HYSAKGIGFMIAIHLAVFSSVYAIVGNISYISFVLKGKLKSAGGSVAHLGFGLIILGI
                     LISSGKKKIISWNKTGIAVFKKTAQEDPAENTTLFKGIKTDMHKYMVTYTGDKFDSSD
                     RKRYYEIDFSPKNGSKGFKVYPNIIKNNKGDGFSPNPDAKHFLTKDIFVYITSFKDDE
                     RENDTTTFRNTEINAGDTAYFNNGFIVMKKANINPDSKKHLLQANETGIFLDMEVISK
                     EGGKYIAAPGLAIKSTNEVRLIYDTVLAQNLILSFNKVIDAKTGKIEIGVKENDSLTS
                     LLTLKVLEFPMIKLLWLGVIIMVVGLIMSIIHRVKSKPSKV"
BASE COUNT         3711 a         1900 c         1594 g         3869 t
ORIGIN      
        1 atgatttgct ggacttcctg gcgcagcgca tgggctacaa cttctccacc aagatgggca
       61 gctaatggcc ccatatcaat tatataatca gcctctctca tcatcatttc atcatgttct
      121 actacaacaa ctgtatttcc tagttttttt aagtgttgta aaactttaat cagtttttct
      181 gtatctctat tatgtaaacc aatactaggc tcatctagta tatataaaga gtcggtaaga
      241 ttagagccta agcttcttgt taactgtatt ctttggcttt ctcccccact taaactgttt
      301 gctaatctgt ttaaagttaa gtaacccaat cctacatcaa ttaaagtttg taaacgctgg
      361 tgtagttcca ataaaactct ttgtgcaact ttgctttcat tggcagataa ttctaaatca
      421 ttaaaccata cttttaaatc ttttgcttgc atttcgcaaa gctctccaat atgcatatgg
      481 ttaattttaa tatacaatgc ttctggcctt aacctatatc ctttacaatg cctgcatttt
      541 gttctacctc tatacctgct aagcataact ctataatgaa ctttatataa gtttgcttct
      601 acttctttaa aaaaatcatc aataccaagt gcttttccaa aacctttcca tagttgttca
      661 tattgttcat tagttaaatc taccacaggt ttatgaatag gaaatccaac tgcatttttt
      721 ataaactgtt ctttccacca aatcattttc tctcccttcc aaggtgcaac tgcaccatca
      781 aaaacgctta gcattttatc tggtattact aagttttcat caatatctaa agtttgccca
      841 aagccttcgc aaacagtaca ggcaccatag gagttattgt aagaaaataa attttgcgat
      901 ggctcttcaa acctcattcc atctaactca aacctattat taaattggta atttttgttt
      961 gtattagtaa ccacattaac aattccatct ccctcataaa aggctgtaga aatactatct
     1021 gttaatcggt gtatatcttc ttcttcaaat tcttcacaaa caattctatc aattaatact
     1081 tgaaaatttg aggcagtttt ttctttaaat gttttattat ccagctccaa taatgcttca
     1141 atctttaata tttctggctc tgttgtggta gaattgttat tcatagcaac tcttgtaaaa
     1201 cccttttgta gcaatatgtt taactcttct cttgtatttc tttttgcatg ttgtttaaat
     1261 ggtacaataa taaaaacttt agtgccttta ttaagttgct gaataacatt taatacatct
     1321 gccacatcct gtttctttac tatgtcatta ctaattgggc taacagtttt accaatattt
     1381 gcatacaata gtttcaaata atcatacatt tctgtcatgc tgccaacagt acttcttgga
     1441 gtacgtgtta taactttttg ttcaattgct atagcaggac ataagccatt aatataatcc
     1501 acatctggtt ttgccatacg ttccataaac tgccttgcat aagagcttaa actctctgca
     1561 taacgcctct gcccttctgc aaacaaagta tcaattgtaa gcgatgattt gccactgcca
     1621 ctaacgccag ttaccacaat taatttattt cttggaatag ttacagaaac attttttaaa
     1681 ttatgcatcc ttgcaccatg aatatatata tcctgcaaag atttatgtgt actggcattc
     1741 tctttgctta tggttgtggc tttattcata caatcaaaat aacaatttta ttttcaaaac
     1801 aatgtaatgg gttacaccga aatatttttt aatgtatagt tatcaggtgt agccaacaat
     1861 gtagcactgt atgtgtcaca cccttcctct gttttttctc tatttaactt ttaatagtaa
     1921 gaaaattttg gtaataatta caaataaaaa agcagagata ctttttacat atctctgcct
     1981 tcattactta aaatattttt taactgcaac ccatactgtt gccacagttt aagcatttat
     2041 agcaagtgcc acttcttaca gtaatgtgca tacatacatt acaagcaggt gcatcgcttt
     2101 gcatattttt tgcagctgca ttaactgcat ctatgccggc ttctgctttt acagttgctg
     2161 ttgaggttac ttggtgtttg tgattttttg gagaagctgt tattctaaca ctactcaact
     2221 cctgttgttc gttaaattca tcatcattac ctaagtttcc aatttctggt ttatctaaaa
     2281 catgcactaa atctgttctg cctaaatatt cataagccaa tgctctaaat acaaagtcaa
     2341 ctaaagaggt agtagtttta atgtttggat gatctaccat tcctgctggt tcaaaccttg
     2401 taaacacaaa tttttctaca aattcttcta aaggcacacc aaactgtaaa ccaaccgata
     2461 cagcaatggc aaagcagttc attaagcttc taactgttga tccttcttta gccaagtcaa
     2521 taaatatttc ccctaaagtg ccatccccat attctcctgt tcttaagaaa ataacttgtc
     2581 cattaatttt tgctttttgt gtaaagcctc ttctttttgc tggtaaagat ttcctttcaa
     2641 ctattctaga aagttggcgt tttaaagttg tatcggtaga ggcattcatt cttttagtaa
     2701 cctcttctaa taaatcttct acagatagtt tagaaagatc tacaatatta gaagcttcgt
     2761 taaccattaa ttcttcttta ttgtcttctt gtgttgtttc tgtagtagta tctacttttt
     2821 tctttttgtc tgatttagta cttaacggtt gcgataactt acttccatct ctatataaag
     2881 cgtttgcttt taaaccaagt tcccagctta gtttatagca ttctgcaatt tcttctacag
     2941 ttgcttcgtg tggtaaatta attgtttttg aaattgctcc acttaaaaat ggttggcagg
     3001 ctgccatcat tttaatatgt ccatgtgcat gtatataacg aactcctttt ttaccacatt
     3061 tattagcaca atcaaaaaca ggtaaatgct cttcttttaa atatggtgct ccttctacag
     3121 tcattgtgcc acatacataa tcattagcag catctatttc ttcatcgcta aaacctaaag
     3181 cttctaataa gctaaagctc cagtcgttat attgttcttc tgtaaatcct aatcgttgta
     3241 aacaagcttc tcctaaaata aatttattga atacaaaacc aatttcgaaa gccgattttg
     3301 cagcagtatt tagtttatta acttcatcgg ttgtaaatcc ttttgcaatt aatatttgag
     3361 gattaataaa aggtgctcct tcaaaacttg cagcaccgac tgcatatttt tcaattgcat
     3421 ctatttggtt ttgtgtatat cctaaatttt ttaaagcatt aggaacagca ccattaatta
     3481 ttttaaaata tccaccgccg ctaagttttt taaatttaac caaagcaaaa tctggctcta
     3541 caccagtggt atcacaatcc attactaaac cgatagttcc tgtaggtgct attactgtgg
     3601 tttgagcatt tctataacca tatttttcgc ccatttgtac tgcatcatcc caggcttttg
     3661 ttgcagcttt caataaatca tcgggacaat attttgcatt aatgccaatt ggttttacac
     3721 taagcccttc ataagcttca ttagcatcgt aagctgctgc acggtggtta cgcattactc
     3781 gtaacatatc taccctattt tcttcgtaac gaggaaaggt gcctaaaaac gaagccatct
     3841 ctgccgatgt tttataagta acaccagtca taactgcact taaagaacct gcaatagctc
     3901 ttgctggttc actatcataa gcaataccac taagcattaa aatagttcca aggtttgcaa
     3961 agccaaggcc taatgttcta taatcgtaac ttaattgggc tacttcttta cttggaaact
     4021 gagccattaa tacagagatt tccaaaacaa ctgtccataa cctgcttata tattctaccc
     4081 cttcaatatc tattttattg gtttgttcat caaagaattt tcttaaattt atagaggcta
     4141 aattacatgc agtattatct aaaaacatgt attcgctaca agggttgctt gcattaattc
     4201 ttccaccttg tgggcaagta tgccattcgt taattgtagt atcgtattgt gttcctgggt
     4261 ctgcacaacg ccatgcagca taagcaattt ggttccatac ttctttagca ggaatttttt
     4321 tcatcaccct gccatctgtt cttgctttta attcccaatc tccgccttct tctaatactt
     4381 taaaaaattc gttaggtatt cttaatgagt tattagagtt ttggcctgct actgtagagt
     4441 aagcttctcc ttcatagcta ttgtcatatc cagcagcaac caaagcagct actttctttt
     4501 cttcttctac cttccagtta ataaattcca tcacttccgg atgatctaaa tctaaacaaa
     4561 ccattttagc tgctctacgg gttgtacctc cactttttat ggctccagca gatctatctc
     4621 caatttttaa aaaactcatt aaaccactag aagtaccacc accacttaat ttttctccac
     4681 taccacgtat ttgagaaaaa ttagttccaa cgccagaacc atatttaaaa attcttgctt
     4741 ctctaatcca taaatccata ataccaccat cattcactaa atcatcatta acacttaaaa
     4801 taaaacaagc atgtggttgt gggcgttcgt atgcattggt agattttttt aattgtccgt
     4861 ccactgaatc aacatagtag tgaccttgtg gcttacctgt aattccataa ctttcatgta
     4921 aaccagtatt aaaccattgc ggactattag gaacacaact ttgatttaat atactataaa
     4981 ctaattcttc gtaaaaaatt tgtgcatctt ctgaggtttc aaaataatcg tatcgttctc
     5041 cccaaacttt ccaacagttt gccatacgat gggcaacctg ttttacactt gtttctctac
     5101 ccaaagtgcc atcttgttgt ggaacacctg cctttctaaa atacttttgt gcaagaatat
     5161 ctgtagctat ttggctccat tgcttaggaa cttctacatt attcatctca aaaactactt
     5221 ctccattagg atttttaatt acactggtac gatagtcgta ttcaaacaga tcgtatgggc
     5281 ttataccatc ttgtgtgaat ttacggctga actgaagacg tttggttttt ttgttgttag
     5341 ccatttgtga tagaatttat gggtggttaa tgatgtttta taaattttgc ctttctaaac
     5401 aaaaaagttt agtggttaac gaaaatattt gttgcacttc aattttccgt acctataaaa
     5461 tccacaggtt gtggaaaagg aatgttaatt ttttggtaac tcaaagccac tttgagtttg
     5521 ctaagttttt aacatttgag gtagaataac ttaaaaaata tttatggaaa attcatctat
     5581 ttctcataat aaagcatatt ggaacaaatt actttgttta tataattgtt attaaaccag
     5641 ttaattaaat ttattcttct taaaagttaa ttttatagca tttattgtat atttttaacc
     5701 aaacttggaa taagcaatac ttttttgcat ttttgcaata ccatttattt aactaattgc
     5761 atgaagcagg atttatacaa tacattcaga aaaaataaat tacaaagaaa aaaatctttt
     5821 actgtactta tcgaccctga taaagtaaat gaacaatctt tagataagct tgtaatgctt
     5881 gcagtggatg ccaaagtgga ctattttttt gtgggtggat cgttggttat atccaatcat
     5941 ttagatgatt gtataaagca attaaaagca aaatgttcta ttcctgttgt actttttcct
     6001 ggttcacctt ctcaagttag taagtatgcc gatgctcttt tatatctttc tttaatttct
     6061 ggacgtaatc cggaattgtt aattggccag catgttgtta gtgctccttt tgttaaaaaa
     6121 agtgggttag agattatgcc aactggttat atggttattg atggtggagc tcctacaaca
     6181 gtttcgtata tttcaaatgc cacacccata ccttctgata aaaatgatat tgcagtttgt
     6241 acagctatgg ctggtgaaat gcttggcatg aaattaattt atatggatgc tggtagtggt
     6301 gctaaacgcc caattactga atctatgatt gaaaaagtgg caagcaacat tgaatctcct
     6361 ttaattattg gtggcggaat tattgatgca gaaaaagcgt atagaaattg tagtgcaggt
     6421 gccgatgtta ttgttgtagg aaatgcaata gaaaaagaca cttcacttat aaaagaaatt
     6481 gctgctgctg tgcatagtaa ttctgttact atataatcaa ttctttaaag caaattttgt
     6541 aacaaaacct taacgctaaa aagtatgttg tagcactaat tgtgttgtct atataggtat
     6601 tataaaccta ttaaattaaa ttctatgaca cagtttatct taaacatacc tactgaaaaa
     6661 gtgcaacagt ttttaattac aatgactgca acaggttttg ctaataccat aaaaaatgta
     6721 ttaaaagaaa aaaattacaa cagttttaat ctactaccat taaataacga tttgcgtaaa
     6781 caacatcctt attttgattc agattttttt gcaaacgatg ttttttatta atccatattt
     6841 tttatattaa ttgaattaaa aattgtaaaa acaaattctt acaatttgaa tattatcttt
     6901 gccctgtatg gatatacagt gcacagaaca agagttatgt attttaaaaa aaattacaaa
     6961 tgccactagt caattaaata caaaaagtta tttaattggt ggatttgtac gagataaaat
     7021 tattggaaga cctaccaaag acgcagatat tgtttgttta aataatggta ttgaattagc
     7081 acacttaaca gctacatttt ttaaacctac ccccaatgta gcttacttta aaacatttgg
     7141 tacagcacag ttaaaaatta accttcatac aaattcatct aaaaacaatt ctacaagttt
     7201 tgaaatagaa tttgctagtg ctagaaaaga aagctacact attgaaagta gaaaaccaaa
     7261 agtagcaaca ggcactttag aagatgacca aaacagaaga gattttacta ttaatgcttt
     7321 agcaattagc ttaaatacag aagattttgg gaaattaatt gacccattta atggattatt
     7381 acatatagag caaggtatta ttaaaacacc attagaacca tccaaaacat ttagcgatga
     7441 cccattaaga atgatgaggg ctatacgatt tgctacacaa ttaaacttta caattgtacc
     7501 cgaagctttt gaggcaatta ttgccaatgc caatcgtatt aaaatagttt cgcaagaaag
     7561 aattacagat gaattaaata aaataatgtt gagcaaaaag ccatctattg gctggcactt
     7621 attaagcaaa tctggtttgt tagaaataat ttttccgcaa atgatggctt tacatggaac
     7681 agaatatgta gataataaag ggcataaaga taatttttat cacaccttac aagtacttga
     7741 taatatttgt gaacacacac aagatttatg gcttagatgg gctgcattgc tacatgatat
     7801 tggaaagcct gcaactaaaa aatttgaaga tggtttaggc tggacatttc actctcatga
     7861 gattgttggt gcaaaaatgg ttcctaaaat tttcaaccaa ttaaaacttc cattaaatga
     7921 aaaaatgagg tttgtaaaaa aattagtagc actgcatttg aggccaattt ctttaacaaa
     7981 agaaaatatt accgatagtg ccattagaag attattgtac gatgcaggag aagatattga
     8041 tgctttaatg cttttgtgta atgcagatat aacgagtaaa aacaagcaaa aagtagaaaa
     8101 atatttaaac aattttaagc ttgttgaaga acgcatgaaa gaagtagaag ctaaagataa
     8161 tatacgtaat tggcaacctc ctataagtgg agagcatatt atgcaaacct ttgctttaac
     8221 tccatctaga gaagttgggc ttattaaaga tgctataaaa gaagctattt tagatggtaa
     8281 aattgaaaat aattatgctg ctgcatacca atttatgttg ctaaaagcaa gcgaattaaa
     8341 cctgcatcca aagcaataac tagtttttta ttattagcaa atttgtttaa aaattttcaa
     8401 cttatattta caaacaaacc aaacacatgg cagttttaaa atttagaatt tatttagaag
     8461 aagacgatgc tgtttataga gatattgcaa taaaacatac ccaaactttt gaacttcttc
     8521 attttactat tctaaaagct tttgaatttg atagtaaaca cgctgcaact ttttacagaa
     8581 gtaatgacaa ttggcaaagg ggtagagaaa taacttttga aaaatatgat aaagaatatg
     8641 ctgcacctcc cttatttatg caagaagttc caataggcaa tgaaataaaa aataccaatc
     8701 aaaaatttat ttatgaatac gattttacca aaggttggat atttttagtt gaactaataa
     8761 gcgtaaataa agaagaaaaa actaaagagg agtatccttt aataactcgt aaagaaggat
     8821 taggccctca acaatatggc accaaaagcc ttttaggcga taagttttct gatattgaag
     8881 agagatatga tttaaccgaa acagcagaag gttttggcaa cgaaggagat agtgaagatt
     8941 cggaaaacga aatgaatgaa gatgcaatgg ataatgataa tgaagaatta taacccgtta
     9001 taaaacatca ataatattga ttgtaatttt aacttattaa tataacgtgc agcttaaaaa
     9061 tactgtaatt ataatttgtg gccctacagc aattggtaaa acaaaattag ccattgaaat
     9121 tgctttacac ttacaaacaa aaataatttc tgccgatagc agacaatgct ttaaagagtt
     9181 aaatattggt gttgcaaaac caagtttata cgaattaaat ttagtagagc atttttttat
     9241 taactctaat tcaataaagg aagataccaa tgcagtcagt tttgaaactt atgctttaaa
     9301 aaaagttaat gcaatatttc aatctacaaa ttatgctgta atggttggtg gaactggttt
     9361 atatataaaa gccttttgcg aaggattaga tgaaattcct gcaataccaa cagaattgag
     9421 aaatgatatt tatacaaatt ataaagccaa tggcttaaca tggcttcaaa aacaactaca
     9481 agcaaaagat aaaaactttt gggaaattgc agaacaacaa aatccccaac gtttaatgag
     9541 agcattagaa gtagttgaat ttactggaaa atctattaat gaatttagaa aacgtaaaca
     9601 acaatccaga gattttaata taattaaaat tggattagaa acagaaagag aaaaattgta
     9661 tagcaatatt aataatcgtg tagatgaaat gatacaaatg ggcttagtag aggaagtaaa
     9721 aaatttatta ccttttgcta attataatgc tttgcaaaca gtaggctata aagaaatatt
     9781 tgattattta aatggaaatt gttcattaga ttttgcaata gaaagtatta aacaacacac
     9841 cagaaactac gctaaaaggc aaattacctg gtttaaaaaa gataaagata ttaaatggtt
     9901 taaccaaaat gaatataatg aaattatagc ttatataaat gcagttaaac cttagaaggt
     9961 tttgatttaa ctctatgtat aatactcatt attaacccaa caaccattat aataactcct
    10021 aaccataaca attttatcat cggaaattct aaaactttta aagtaagtaa agatgttaat
    10081 gaatcatttt ctttaacacc aatttcaatt ttacctgttt ttgcatctat aactttatta
    10141 aaactcaata ttaaattttg agctaatact gtatcatata ttaatcttac ttcgttggtt
    10201 gatttaatgg ctaaaccagg tgctgcaata tatttacctc cttctttact tataacctcc
    10261 atatctaaaa atatacctgt ttcgttagct tgtaacaaat gctttttaga atcaggattt
    10321 atgttagcct ttttcattac aataaagcca ttgttaaaat aggcggtatc tcctgcattt
    10381 atttctgtat ttctaaatgt tgttgtatca ttctctcttt catcatcttt aaatgaagtg
    10441 atataaacaa agatatcttt ggttaaaaaa tgttttgcat caggattagg agaaaaacca
    10501 tcccctttat tattttttat gatattaggg taaactttaa atcctttact accatttttg
    10561 ggagaaaagt caatttcata atatcgtttt ctatcgctac tatcaaactt atctcctgta
    10621 taagtaacca tgtacttatg catatctgtt ttaatacctt taaaaagtgt agtattttca
    10681 gctgggtctt cttgtgctgt tttcttaaat actgcaattc ctgttttgtt ccaactaatt
    10741 atttttttct ttccactcga aattaatata cctaaaatta ttaacccaaa tccaagatgt
    10801 gcaacactac ctccagccga ttttaatttt ccttttaaaa caaaagatat ataactaata
    10861 ttaccaacta ttgcatatac tgaagagaat acagccaaat gtattgcaat cataaaacca
    10921 attcctttgg ctgaataatg cactttacca acaaaaataa ttagcaatgc aacgattaag
    10981 gaaatgaaaa caggtagcca gaattttttg ccaataaatg atttgggtgt gtttttatat
    11041 tttaaaaact gaacagcacc agttaacaac ccta
//
LOCUS       DAOQPK010000052        11072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_3048_length_11072_cov_4.857130, whole genome shotgun sequence.
ACCESSION   DAOQPK010000052 DAOQPK010000000
VERSION     DAOQPK010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11072
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3048_length_11072_cov_4.857130"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2214)
                     /locus_tag="PLU36_04000"
     CDS             complement(<1..2214)
                     /locus_tag="PLU36_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45944.1"
                     /translation="MKYIFTTLFVTLLFIKVNAQINPVGSYDSCGAALNLDVNATCIT
                     GGQTYMTTGATKSPQATLGISNDDDVWFKFTTLAGQTHASIQISNEIGGMGSCMELWD
                     SCNASAEISDFCGSQFNVSGLTPLKTYLVRVYTNGTFSTLSSFQMCVVNTTPPPPPPN
                     SECAFATSIQVSNNNAVCNLFPLTNNYATGSTIVSAPACLSSNYKDIWIKFVPSQTGP
                     LSFRMQDYSAIAGSAFPTYYIATYSGTNCNSLSLVNCSNYSPTSGNDYSLSGTYNSGS
                     TYYLRILCDNSIAGDFNVCLRPSVSETNYPISADSTCLRSIAINSSVDNSALYTQGST
                     NGIRRINQLACYGYNAPNALAWYSFTVPSNGNYFVDFTDLTRLDVNAFGAGYRVLRRA
                     TCYSTGTDTIITKPPNSTYDTLLCANSFDNGQMLTLTTGTQYYVTVMENSYNGGRVAY
                     KLRVIGASTCVNDDSTNAINIIQDVNCNNAINTSLRFSTLSTQPNISGLTNNGSLIQD
                     VWYKFNAITSSVIINVTLPLYSSRIVVYNSDGTIKYDPGTNGNIITVNSLVIGARYLL
                     RVFNTNGLPIGPSADFKICVFGASPSVATSVSSCTPADATNISTNSNQWLHFQRSGGL
                     IVSVFDGPAKPGFTFLPRGTISVSYFTNNGGIRLNAGTSYLDRNFEISDGGNNFSNSP
                     VRVRFYLEPGEFNTLVASAASGGISAPYELKVYRIPGASCSNSTTAGGLYYNIDSY"
     gene            complement(2394..3050)
                     /locus_tag="PLU36_04005"
     CDS             complement(2394..3050)
                     /locus_tag="PLU36_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ45945.1"
                     /translation="MQINNELNIRITIFDDNEAICNSLYQLLHYTPGFEVVSFFKNCE
                     NVADKVEECLPRVILMDINIPPTSGIEAVKEIKRKFKTVDIIMFTVFEEEDKIFEALK
                     AGATGYLLKKTPPLKIIESIQEVVAGGSPMSASIARKVMNFFTQQSVLQNNKNILTRR
                     EQDVLKGLIDGQSYKMIAINLSISIETIRTYIKRIYDKMHVNSMTEAVAKALKEKWFE
                     "
     gene            complement(3094..6078)
                     /locus_tag="PLU36_04010"
     CDS             complement(3094..6078)
                     /locus_tag="PLU36_04010"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HMZ45946.1"
                     /translation="MPKLFLFIITLLINKNINAQKQDELICRHINNQNGLMYGHLTSI
                     AQDKQGYIWITGDEGLQRYDGYEFINYLHNPTTPYETLPNGRLTKVAVDYQNKLWIGS
                     FSNGFGYYNSFNNTYKLYLPKNQPHLSPNAIGFRSFLFSNDSVTYACSTDGLVKLLNG
                     EIVKVFTTNNSSLQGSLIGRIAKDRNNNIWIGSLGGLNMLTSDENNLYNHTNNKTITA
                     FSNNVLKDNTGNKAAIADLFIDGKNNLWISSWKPELYCYNISTNSFKTIKLPNPKPFI
                     YDNMAQCFVEDNNGNLWIGTSNNGVYKYNYSEDKFTHYIHDRSNNRSIASNSINCMLK
                     DYQGNIWLVSDNNISIFNPSDLPVKIILANTAISATMLAKDKSLWAADNNYLFHFDSS
                     LNLLNKIKHNQDKINNVWKVKESINSDEIFILKEKGLTIYNKMNGSIEPFKDLPILKN
                     NPITDIIELNNGNLFLLRWWWDKNLLYLDRAKRKISAVNVPLFDKNNFEISCAIKKDS
                     INYYLFSKKGLMLLNTSTKMVNTLDSNFHAGPTILINNQFYSATSSSGIRIYNLYSKH
                     ISEISKYNGLPINNTKSIIYAGNNEFWIGGTTGIIKWNQQRNSFNTFRENYGINSDNI
                     AENSLSVMPDGELVFNDGSLKLLASENIKKFPPPKVSIIQCIAGDSELTLEKLNGKVV
                     INHNNNILQFKYGAINYNQNIQYHYKLEGHDKTWKIGNSRIVNYTNLAHGNYVFYVKA
                     ITEDGRWNNEITKVNIYVTQAFYKAIWFYALVAIIVICTILFYYRIKINNLKNLQKLR
                     NNISKDLHDEIGSTLSSISILSTSVINNIEKEKQKSKEWVEQIGNNAQSMLNAMDDIV
                     WSINPNADTFESIILRMKELANANSEPLSIEVNFHYDEELKHVSLPMLIKKNIFLIYK
                     ECINNSLKYSGTKSIDILLKKKKSKLFLTITDYGIGFDINKEYNRNGLKNIKHRAHEI
                     NAELVLQSNKEKGTSTQLIINL"
     gene            complement(6242..7315)
                     /locus_tag="PLU36_04015"
     CDS             complement(6242..7315)
                     /locus_tag="PLU36_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HMZ45947.1"
                     /translation="MQFRNFKMVGYVVYGRGCFNQLNEILAPHRKNNAPIIFLVDHFF
                     ECKPLINKIPLQGNDKIIFANVTYEPKTTQVDSIAEQLKNEFGTVSGIVGIGGGSTMD
                     LAKAVSLMMNNPGSSADYQGWDLVKQAGVYKVGIPTLSGTGAEVSRTTVLTGPARKLG
                     MNSDFTPFDQIILDPELTKNAPGNQRFYTGMDCYIHCIESLEGTFLNEFSKSYGEKAL
                     QLCRDIFLGKETWDDECDDKLMMASYAGGMSIAYSQVGVAHAVSYGLSYVLGTKHGIG
                     NCIVMNHLEEYYPQGHKEFKKMVDKGGYEIPNGICSNLTEEQFESMMNVSLSLKPLWE
                     NALGKNWEAIMTKQKLRKLYERL"
     gene            complement(7492..7564)
                     /locus_tag="PLU36_04020"
     tRNA            complement(7492..7564)
                     /locus_tag="PLU36_04020"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7529..7531),aa:Phe,seq:gaa)
     gene            7761..7973
                     /locus_tag="PLU36_04025"
     CDS             7761..7973
                     /locus_tag="PLU36_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HMZ45948.1"
                     /translation="MFIPKLLLGMPGGSEWIIIILAVLILFGGRKIPEFMRGIGKGIR
                     EFNDAKNNVKKEIEDGMNEKDKNSSN"
     gene            8014..9450
                     /gene="gatA"
                     /locus_tag="PLU36_04030"
     CDS             8014..9450
                     /gene="gatA"
                     /locus_tag="PLU36_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HMZ45949.1"
                     /translation="MFTFSTIKDYHIALLNGNTTCVSTVNHYLQAIKDSYHLNAFLEV
                     FECEAIDIAKKLDLNRQQGMPLGKLHGVVVALKDVIIYKGHRASAASKILENYISIYN
                     ATVVEKLLAQEAIIIGINNCDEFAMGSSNENSAFGNVKNALDTTRVSGGSSGGSAVAV
                     QAGMCMISLGSDTGGSVRQPADFCGIVGLKPTYGRVSRYGLIAYASSFDQIGIFANSV
                     EDAALTLEVIAGEDEFDSTVSSLPVPSYSTLLEQAKDQKFSIAYFKETLDNPALDPEI
                     RDTFYSFIKTLTENGHSVQVVDFEYLEYIVPTYYILTTAEASCNLSRYDGVRFGHQTQ
                     HINATLTDVYKATRSIGFGKEVKRRIMLGAFVLSEGYYDAYFTKAQQVRNLLLQKTNT
                     IFEKFDAIISPTVPTVAWKFGEKSNSATEMYLADIYTVYANLVGIPAISLPLFKHSNK
                     MPFGLQIMVSSNKEVILHSLSKNLIKLK"
     gene            9528..10625
                     /locus_tag="PLU36_04035"
     CDS             9528..10625
                     /locus_tag="PLU36_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HMZ45950.1"
                     /translation="MQWLLNMIGYPKNIQKTAIVLVGLLVLGFGAVSFTKADIQEYRG
                     RGFYALNDTNSNSLIAELAPKNYYTQNAISVDINPLAVGFMQQYADKQGKWFEKMKQW
                     GKPYLDLYDQILPQYGIPKELKYLSVIESNLNPGTISSAGAVGPWQLMSYEGRKYGLR
                     MTEYNDERMDFYKSTHAACKLLNELRNTFNDWLLVIAAYNGGVGRVKQAMKKAKSKNF
                     WDIQYYLPLETRNHVKKYIATHYIFEGSGGWTTMTEQETNQYIASFALETKQSALTDE
                     EIKNTAIVEVGGRFLSVIIANNLLLDIDQFNKWNPKFDKTLAEGKKYSMRISIDKLPL
                     FEAKKSQILLESMNVLLNDNALVNNRKLQNN"
     gene            complement(10627..>11072)
                     /locus_tag="PLU36_04040"
     CDS             complement(10627..>11072)
                     /locus_tag="PLU36_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ45951.1"
                     /translation="IKEVYESLSIKTNKYNFNTSIKKGCDQPIIVFADKEKIRQVITN
                     IISNATYYGKQNGSIVASIYNTDETNVLVEFSDDGKGISEEHLPRVFERFYRTDKGRS
                     RNDGGSGLGLAICKHIIEAHNQTIHIRSTVDVGTTIGFTLGSAVN"
BASE COUNT         3741 a         1760 c         1597 g         3974 t
ORIGIN      
        1 atagctgtca atattgtaat ataagccacc tgcagttgtt gaattactgc aagatgcacc
       61 cggtattcta tacactttta attcataagg tgcactaata ccaccacttg cagctgaagc
      121 tactaatgta ttaaactctc ctggttctag atagaaacga actctaacag ggctattact
      181 aaaattatta cctccatcgc ttatttcaaa atttctatct aaatatgaag ttcctgcatt
      241 tagcctaatg ccaccattat ttgtaaaata gcttacagaa atggtacctc tgggtaagaa
      301 tgtaaaacct ggttttgcag gtccgtcaaa aacagaaaca attaacccgc cagatctttg
      361 aaaatgtaac cattgattac tgttggtaga aatattagtt gcatcggcag gtgtgcaact
      421 gcttacactt gttgcaacag aaggagatgc tccaaaaaca caaattttaa aatctgcact
      481 tggaccaatt ggcaaaccat ttgtattaaa aactcttaat aaataccttg ctccaattac
      541 taatgaatta acagtaataa tatttccatt tgtaccagga tcgtatttaa tggttccatc
      601 tgaattatat actactattc tagaagaata taatggtaat gtaacattta ttataacaga
      661 ggatgttatt gcattaaact tataccaaac atcttgaatt aggctaccat tattagttaa
      721 accagaaata ttgggttgtg tacttaatgt actaaatcgt aaacttgtat taattgcatt
      781 gttacaattt acatcctgaa taatattgat agcattggta ctgtcatcat ttacacaagt
      841 tgaggcacca attaccctaa gcttataagc tactctacca ccattatacg aattttccat
      901 tacggttaca taatattgag ttccagttgt taatgttaac atttgaccat tgtcgaaact
      961 gtttgcacac aacaatgtat cgtaagtact attaggaggt tttgtaatta tagtatctgt
     1021 tccagttgaa taacaagtag ctcttcttaa cactctataa cctgcaccaa aagcatttac
     1081 atctaaccta gttaaatctg taaaatcaac aaaataattt ccattggatg gcactgtaaa
     1141 actataccat gctaatgcat ttggtgcatt gtatccatag caagcaagtt gattaattct
     1201 tctaatgccg tttgttgatc cttgtgtata taatgcacta ttgtcaacag aagaattaat
     1261 tgcaattgaa cgtaaacatg tactatctgc agaaatagga taattagttt cactaacaga
     1321 tggtcttaag caaacattaa aatctcctgc aattgaatta tcacataata ttctaaggta
     1381 ataggtagaa cccgaattgt atgtaccaga taaactataa tcgtttccag atgtgggtga
     1441 ataattagaa cagtttacaa gtgataaact gttacaattt gtaccagaat aagtggctat
     1501 ataataagta ggaaatgcag agccagcaat ggcagaataa tcttgcattc taaatgataa
     1561 ggggccagtt tgagaaggta caaattttat ccaaatatct ttatagttac tagataaaca
     1621 agcaggggca ctaacaatag tactacccgt ggcataatta ttagttaatg gaaataaatt
     1681 gcaaactgca ttattattac taacttgaat agatgtagca aaagcacatt cactatttgg
     1741 aggaggtggt ggtggtgttg tatttacaac acacatttga aaagaagaaa gagtagagaa
     1801 agttccattt gtataaaccc ttactaaata agttttaagt ggtgttaatc cagaaacatt
     1861 gaattgactt ccacaaaaat cactaatctc tgcagatgca ttacatgaat cccacaattc
     1921 catacaggaa cccataccac caatttcatt tgatatttga atactagcat gggtttgccc
     1981 agctaaagtg gtaaacttaa accaaacatc atcatcatta ctaattccta aggttgcttg
     2041 tggacttttt gttgcacctg ttgtcatata agtttgccca cctgtaatac aagtagcatt
     2101 tacatctaag tttaatgcag ccccacagct atcgtagctg ccaacaggat ttatttgtgc
     2161 atttactttt ataaatagta aagtaacaaa aagtgtggta aatatgtatt tcattttatt
     2221 aattttttaa atttatattt tctatccact tttagcccta atttctaatt aataattaag
     2281 aagagcttta aatgtgaagt gatgttagta taaaagaatt aacctataac aaaggtattt
     2341 ttgttataag attattacaa taccataaaa gggggacaat gtttgattaa aacttactcg
     2401 aaccattttt cttttagagc ttttgcaaca gcttcggtca tagagtttac atgcatttta
     2461 tcgtaaatgc gtttaatata agttctgata gtttctatac ttatacttaa attaattgca
     2521 atcattttgt aactctggcc atcaattaac ccttttaaaa catcttgttc tcttctggtt
     2581 aaaatatttt tgttgttttg caaaacagat tgttgagtaa aaaaattcat tacctttctt
     2641 gcaattgaag cactcattgg agacccgcca gcaactactt cttgtattga ttctataatt
     2701 tttaatggag gtgttttttt taataaataa cctgttgcac cagctttaag ggcttcaaaa
     2761 attttatctt cctcttcaaa taccgtaaac ataataatat ctacagtttt aaattttctt
     2821 tttatttcct ttactgcttc aattccagaa gtgggaggaa tattaatatc cattagtata
     2881 actcttggta aacattcttc aactttatct gcaacatttt cacaattttt aaaaaaacta
     2941 acaacttcaa accctggagt gtaatgtaat aactgatata atgaattaca aatagcttca
     3001 ttatcgtcaa atatggtaat tcgtatattc aactcattat ttatttgcat aaaagtatta
     3061 aatgtttaag ttaattgaag taccataaat gggttacaaa tttattatta gttgtgtgct
     3121 tgttcctttt tctttattgc tttgtagcac taactcagca tttatctcat gtgccctatg
     3181 ttttatattt tttaatccat ttctattata ttctttgtta atatcaaagc caattccata
     3241 atccgttata gttaaaaata attttgattt ctttttctta agcaatatat caatactttt
     3301 tgttcctgaa tattttaaag agttattaat acattcctta taaataagga atatattttt
     3361 cttaataagc ataggtaggc ttacatgttt taactcttca tcataatgaa aatttacttc
     3421 aatagataat ggttctgaat ttgcatttgc aagttctttc attcttaata ttatactttc
     3481 aaaagtatct gcattaggat taattgacca cactatatca tccattgcat taagcataga
     3541 ttgtgcatta ttaccaattt gctctaccca ttcttttgat ttttgttttt ctttttcaat
     3601 attatttatt acagatgtac ttaaaattga aatactgcta agtgttgacc ctatttcgtc
     3661 gtgtaaatct tttgaaatat tattacgaag tttttgcaaa ttctttaagt tgtttatctt
     3721 aattctgtaa taaaataaaa tagtacaaat tacaataatt gcaactaaag catagaacca
     3781 aattgctttg taaaaagctt gtgttacata tatattaact ttggtaattt cattgttcca
     3841 tcgtccatcc tcagtaatag cctttacata aaaaacataa tttccatgag caagattagt
     3901 ataatttaca atcctgctat taccaatttt ccatgtttta tcatgccctt ctaacttata
     3961 atggtattga atattttggt tgtaattaat agctccatat ttaaattgaa gtatattgtt
     4021 attatgatta attacaactt taccatttag tttttcaagt gttaactccg aatctccggc
     4081 aatacattgt atgattgaaa ctttaggtgg tggaaatttt tttatatttt cagatgctaa
     4141 taactttaat gaaccatcat taaaaaccaa ttctccatct ggcattactg aaagagaatt
     4201 ttcggcaata ttatctgaat ttataccata gttttctcga aatgtattga atgaatttct
     4261 ttgctgattc cattttatta tacctgtagt acctccaatc caaaattcat tatttccagc
     4321 ataaataata ctcttggtat tatttatagg taatccattg tatttactaa tttcagaaat
     4381 atgtttgcta taaagattgt aaatcctaat tccagaacta gatgttgctg aataaaattg
     4441 attattaatt agaatagttg gaccagcatg aaaatttgaa tctagtgtgt ttaccatctt
     4501 tgtactagta tttaaaagca ttaatccttt ctttgaaaac aaataataat taattgaatc
     4561 tttttttatt gcacatgaaa tttcaaaatt atttttatca aataatggaa cattaactgc
     4621 actaattttt cttttagctc tatctaaata cagcaaattt ttatcccacc accatcgtaa
     4681 taaaaatagg ttaccattat ttaattctat aatatccgtt attgggttgt ttttaagtat
     4741 tggcaaatct ttaaatggct caatactacc attcatctta ttataaattg ttaagccctt
     4801 ctctttcaaa atgaatattt catcactgtt tatagattct tttactttcc aaacattatt
     4861 tattttatct tggttatgtt ttattttatt taaaagattt aaagaagaat caaaatgaaa
     4921 taaataatta ttatctgctg cccataagct tttatctttt gcaagcatag ttgcagaaat
     4981 tgcggtattt gcaagaatta tttttactgg taaatcagaa gggttaaaga tgctaatatt
     5041 attatctgaa actaaccaaa tatttccttg ataatctttt agcatacaat ttatactatt
     5101 gcttgcaatg cttctattat tacttctatc gtgaatatag tgtgtaaact tatcttcgga
     5161 ataattgtat ttgtaaactc cattatttga ggttcctatc caaaggtttc cattattatc
     5221 ttcaacaaag cattgtgcca tattatcata aataaaaggc tttgggttgg gcagctttat
     5281 tgttttaaaa ctattagttg aaatattata gcaatacaat tctggtttcc aactactaat
     5341 ccaaagatta tttttaccat caataaaaag atcggcaatt gcagctttgt tacctgtatt
     5401 gtcttttaaa acattattgg aaaatgcagt aattgtttta ttattggtgt gattatacaa
     5461 attattttca tcgcttgtta acatatttaa tccaccaagt gatcctatcc aaatattgtt
     5521 atttctatct ttagctattc ttccaatcaa tgatccttgt agtgaggagt tgtttgttgt
     5581 aaaaactttt acaatttctc cgttcaaaag ttttactagt ccatctgtac tgcaagcata
     5641 agttacagag tcgttactaa ataaaaaact tctaaaacca attgcatttg gcgaaaggtg
     5701 tggttgattt ttgggtaagt aaagtttata agtattattg aaagaattgt aataaccaaa
     5761 accattgcta aaagaaccaa tccataattt gttttgataa tccacagcaa cttttgttaa
     5821 tcttccgtta ggtaaggttt cataaggtgt tgttggattg tgtaaataat taataaactc
     5881 atacccatcg taacgttgaa gcccttcatc tcctgtaatc caaatataac cttgcttgtc
     5941 ttgtgctata ctagttaagt gcccatacat taacccattt tgattattta tatgcctgca
     6001 aattaattca tcttgcttct gtgcatttat gtttttatta attaacaatg taataatgaa
     6061 cagaaatagt ttaggcatga gtttattttg ctatataaaa ctattgtttt ttttatgaac
     6121 tttattaaag ttcaacatta aattcgtttt tatatagtta gcattgaata aaaaatgcat
     6181 tgacactttc taaaaaactg tatcaatgca tttaaagagg gttgttattt ttatataact
     6241 gttacagcct ttcgtatagt tttcttaatt tttgttttgt cataattgct tcccaatttt
     6301 tgcccaaagc attctcccat aatggtttta ggctaaggct aacattcatc atactttcaa
     6361 actgttcttc tgttaaattg ctacagatgc cattaggtat ttcatatcct cctttgtcaa
     6421 ccattttttt aaattctttg tgtccctgtg ggtaatactc ttctaaatgg ttcattacaa
     6481 tacaatttcc tattccatgt ttggtgccca aaacataact taaaccatag cttacagcat
     6541 gtgcaacacc aacttggcta tacgctatac tcatacctcc tgcataactt gccatcatta
     6601 atttatcatc acattcatca tcccatgttt ctttacctaa aaaaatgtct ctacaaagtt
     6661 gcaatgcctt ttcaccataa cttttgctaa actcattcaa aaaagttcct tctaaacttt
     6721 caatgcaatg tatataacaa tccattcctg tataaaaacg ctgattacct ggtgcatttt
     6781 tagtaagctc tgggtcaagt ataatttgat caaaaggtgt aaagtcacta ttcattccta
     6841 attttctggc agggcctgtt aaaaccgttg ttctacttac ttctgcacct gtaccactta
     6901 aagtaggaat accaacttta tatacacctg cttgttttac taaatcccag ccttgatagt
     6961 ctgcactact ccctgggttg ttcatcatta acgaaacagc ttttgctaaa tccattgtac
     7021 taccaccacc aatgcctaca ataccgctaa ctgtaccaaa ctcatttttt aattgctctg
     7081 caatggaatc tacttgtgta gttttgggtt cgtaagtaac attggcaaaa ataattttat
     7141 catttccttg caatggaatt ttgttgatta atggtttgca ctcaaaaaaa tgatctacta
     7201 aaaaaataat aggagcattg ttttttctgt gtggagctaa aatttcattt aactgattaa
     7261 aacaacctct tccatacacc acatatccaa ccattttaaa atttctaaat tgcataatta
     7321 tttttttaaa taatgaataa acaaaagtaa ggtttgtttt aaactatgtt ttaaaactat
     7381 caattattaa aaaaaattac caaaatgtaa taaaatagta aaatgtataa gagcctcttt
     7441 aagcttattg aaagaaaaag agaggctatt aaaaataacc tctctttaaa gtggtgtggg
     7501 ccgggatcga accggcgaca caaggatttt cagtcctttg ctctacctac tgagctaccg
     7561 cacctgcctc cctaattggg gttgcaaata taatggcaaa tcctaattca caaaaaaata
     7621 ataattaatt tacaacatac ttttttagaa tacttaaatg taatgataca atagtttatt
     7681 atttacataa aatgagtgaa aattattttg actttattaa gaattttatt gtttaccttt
     7741 gtgtcttata aaagagcaac atgtttatac ctaaattact tttgggaatg cccggtggca
     7801 gcgaatggat tattattatt ttagctgttc ttattttatt tggcggtaga aaaatacctg
     7861 aattcatgcg tggcatagga aaaggtattc gtgaatttaa cgatgcaaaa aataacgtta
     7921 aaaaagaaat tgaagacggg atgaacgaaa aagacaaaaa ttcatctaac taatttctgg
     7981 catttcaata attattaaaa ttacttaaag cctttgttta cttttagtac aatcaaagac
     8041 tatcatattg ctttgcttaa tggcaataca acatgtgtat ctactgttaa ccattattta
     8101 caagcaatta aagactccta tcatctaaat gcttttttgg aagtttttga atgtgaagct
     8161 attgatatag caaaaaaatt agatttaaac agacaacaag gcatgccttt aggaaaacta
     8221 catggtgttg tagttgcttt aaaagatgta attatttata aagggcatag ggcttctgct
     8281 gcatcaaaaa tattagaaaa ttatatttca atttataatg ctaccgttgt agaaaaacta
     8341 ttagctcaag aagctatcat tattggcata aacaattgcg atgaatttgc aatgggaagc
     8401 agtaatgaaa acagtgcttt tggaaatgta aaaaatgctt tagacactac aagagtttct
     8461 ggaggatctt ctggaggttc tgctgtagca gtgcaagctg gcatgtgcat gattagttta
     8521 ggtagcgata caggtggcag tgttagacaa ccagcagatt tttgtggaat tgtaggttta
     8581 aaaccaactt atggcagagt ttctaggtat ggtttaatag cttatgcttc ctccttcgat
     8641 caaattggta tttttgcaaa ctcagttgaa gatgctgctt taaccttaga ggtaattgca
     8701 ggagaagatg agtttgatag tactgtttct tccttaccag ttccttctta ttctactttg
     8761 ttagaacaag caaaagatca aaaattttct atcgcttatt ttaaagaaac cttagataat
     8821 cctgctttag acccagaaat acgagacact ttttactcgt ttataaaaac tttaacagaa
     8881 aatggccata gcgtgcaagt tgttgatttt gaatatcttg aatatatagt tcctacatat
     8941 tatattttaa caactgccga ggctagttgc aacctttcta ggtacgatgg ggtaaggttt
     9001 gggcatcaaa cacaacatat aaatgcaact ttgacagacg tttacaaagc taccagaagt
     9061 attggttttg gtaaagaagt taaacgcaga attatgttag gtgcttttgt acttagtgaa
     9121 ggatattatg atgcatattt tactaaggca caacaagtta gaaatttatt acttcaaaaa
     9181 accaatacga tttttgaaaa gttcgacgca atcatttctc ctactgtacc aacagtagca
     9241 tggaaatttg gagaaaaaag caactccgct accgaaatgt acctggctga catttataca
     9301 gtatatgcaa accttgttgg tattcctgct atttcccttc cattatttaa acactccaat
     9361 aaaatgccat ttgggttaca aataatggtt tctagtaata aagaagtaat tttgcactcc
     9421 cttagtaaaa atttaataaa gttaaagtaa gctttacaac aatttttaaa ggaagttttc
     9481 aaccacacta actcttattt tttatcattt aaacaagtga atagattatg cagtggttat
     9541 taaatatgat aggttatcca aaaaatatac aaaaaactgc tattgtttta gtaggacttt
     9601 tggttttggg ttttggtgca gtttctttta caaaagcaga tattcaagaa tatagaggca
     9661 ggggatttta tgcacttaac gacactaact caaattcatt aatagcagaa ctagctccta
     9721 aaaattatta cacacaaaat gctataagtg tagatataaa tcctttagca gtagggttta
     9781 tgcagcaata tgctgacaaa caaggtaaat ggtttgaaaa gatgaaacaa tggggtaagc
     9841 catatttaga tttatacgat caaattttac cgcaatatgg tattccaaaa gagttaaagt
     9901 acttaagtgt aatagaaagt aatttaaatc caggtacaat ttcttcagct ggtgctgttg
     9961 gcccgtggca attaatgagc tacgaaggaa gaaaatatgg attaagaatg acagagtaca
    10021 atgatgaaag aatggatttt tacaaaagca cacatgcagc ttgtaaacta ttaaacgaac
    10081 ttcgcaatac gtttaatgac tggttattag ttattgcagc atacaatggt ggtgttggca
    10141 gagtgaaaca agccatgaaa aaagctaaaa gcaaaaactt ttgggacatt caatattatt
    10201 taccattaga aaccagaaac catgttaaaa aatatattgc tactcattat atttttgaag
    10261 gctctggtgg atggacaaca atgactgaac aagaaacaaa tcaatatata gcctcatttg
    10321 ctttagaaac taagcaatct gctcttactg atgaagaaat aaaaaatact gctattgttg
    10381 aagttggagg tagattttta tcggttatta ttgcaaacaa cttattgcta gatatagatc
    10441 agtttaataa atggaaccca aaatttgata aaactttagc agaaggtaaa aaatattcaa
    10501 tgagaatttc tattgacaaa ttacctttat ttgaagctaa aaaaagccaa atacttttag
    10561 agagtatgaa tgtattatta aatgataacg ctttagtaaa taatagaaaa ttacaaaata
    10621 attagtttaa ttaactgcag aacctaaggt aaagccaatt gttgttccta catctacagt
    10681 gcttcgtata tgaattgttt gattgtgtgc ctcaataata tgtttacaaa ttgctaaccc
    10741 taagccagat cctccatcat ttctgcttct acccttatct gttctataaa atctttcaaa
    10801 aaccctaggc aaatgctctt cagatattcc tttaccatca tcactaaatt caactaatac
    10861 attggtttca tctgtattgt aaatacttgc cacaatgctt ccattttgtt ttccataata
    10921 agtagcatta ctaataatat ttgtaattac ttgtcgaatc ttttctttat cagcaaaaac
    10981 aataatgggt tgatcgcaac cttttttaat actggtattg aaattgtatt tattggtttt
    11041 tatagaaagc gactcataca cttctttaat ta
//
LOCUS       DAOQPK010000053        11029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_3066_length_11029_cov_4.329779, whole genome shotgun sequence.
ACCESSION   DAOQPK010000053 DAOQPK010000000
VERSION     DAOQPK010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 11029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11029
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3066_length_11029_cov_4.329779"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PLU36_04045"
     CDS             <1..407
                     /locus_tag="PLU36_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45952.1"
                     /translation="TYQQFATTIEKLTQIDFGRFANITRNSDTEKIASKILTSINKTG
                     QQIFIHHFPTLANIDWKITNKIISELSTEYLYNLLRWEKLDLYTVNLPSLKLALDYCK
                     KTEESKFIEGIINKNKYRFDKYRKQNYKKHLP"
     gene            651..905
                     /locus_tag="PLU36_04050"
     CDS             651..905
                     /locus_tag="PLU36_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HMZ45953.1"
                     /translation="MKNTSISLGNYFDHFVSSQVSAGRYKNVSEVVRAGLRLLEDEES
                     KVIALKDAIQKGIDSGIAYDFDPQKHLQEIKAKKRNNGKI"
     gene            892..1191
                     /locus_tag="PLU36_04055"
     CDS             892..1191
                     /locus_tag="PLU36_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HMZ45954.1"
                     /translation="MAKYELTNEAVEDLTKIWDYTIDKWSEKQADSYYEMLLKNCQHI
                     ADDPNIGRNYEGIRNDLFGLKVNRHIIFYRKLSNKPIEITRILHERMDLKNRLNE"
     gene            1415..1768
                     /locus_tag="PLU36_04060"
     CDS             1415..1768
                     /locus_tag="PLU36_04060"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HMZ45955.1"
                     /translation="MNTQRQIIFHKHYFIDFYVEQNEKVQEKIEYVFKILRTVQKVPK
                     KFLDHMTGTDGLYEIRIEFESNIYRIFCCFDKDNLVVLFNGFQKKSQKTPKKEIDLAL
                     KLKDEYFNSKKQSNE"
     gene            1761..2066
                     /locus_tag="PLU36_04065"
     CDS             1761..2066
                     /locus_tag="PLU36_04065"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ45956.1"
                     /translation="MNKENIKNAKNFDELLDIKYGKIGSENRDKFEEKAQYFVISEML
                     KEARKEANMTQEQLAEKVGTKKSYISRLENGKCDIQLSTLYRIFEFGLGKRVNLLFG"
     gene            complement(2349..2984)
                     /locus_tag="PLU36_04070"
     CDS             complement(2349..2984)
                     /locus_tag="PLU36_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ45957.1"
                     /translation="MLTVKSFTFNPIQENTYIVYNQNRKAVIFDPGCFFEEEKNLLKQ
                     FIEENKLVPIQLINTHCHLDHVFGNKFIHDTYGLELYIHPKEQMMLEYAPISGQQYGL
                     SFENYKGNIHFLKEEDKIEIEEEELTILFTPGHSPGHLSFYNRKANFAIVGDVLFYES
                     IGRTDLPGGDYDTLMSSIEQKLYTLPNNTKVFSGHGPTTTIGYEKDNNPFF"
     gene            3132..6506
                     /locus_tag="PLU36_04075"
     CDS             3132..6506
                     /locus_tag="PLU36_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45958.1"
                     /translation="MFFLIGILFSYATIQAQVNTVEFGKNRIQHKKLKWKFYQSKNFN
                     TYMAQGGTEIARFVAQVAEEELPELENFIEYNLHRRADIVVYTNFEDYKQSNIGLGID
                     WQQSGGLTKLVNNKIVVFFDGNHNNLKVKIREGIAKVLTDNLLFGDDIGEFASNQALL
                     DLPQWLTDGYVSYAAENWSTKKDDELKSEILSGNYKNFYNFAYLKPILAGHSFWNFIA
                     EKYKKDNVTYFLYLARMYKNLNTASLKICKKKFKDVLAEFMESKQNQYTEDIRKRKNA
                     TKGKRTISEDVSKHNYYNFQINPNPKNNAYAMVQFKKGMYSVILNDNYYYVKTLLKRG
                     VRVLQGDQSPNYPIMAWDGKGTRLLVIYAEDAKVKLFVWDYVARYKRFKQVLEGVDQV
                     IDASFMLDANTVILSAVKNGHTDIFIYKIQENKLEQITNDVYDDLNPTYVSFPNRSGI
                     IFSSNRPSANAPMEDTAIPSRNRFNVFLVDILNKSATKQITQLSSLQYSNASYPMQYN
                     VNHFTFVADETGIGNRYAGFFSTQRDGLDTLYYIGEDIIRNPTKNELDSSLAAWRIEK
                     PDSVSYFQVYKDSTYTFPITNYQSSILETRIAGNNGMVSEVRREGDYKYLFKLQVDDK
                     ALKNRNINPALSNYMKQVRGIVKQQKGKPNKSKKETTTVKADKPTTDTTKPTNKKVFQ
                     TEFDDSVENTDTANTTNKTTPANTGNKESEKIELKKRLFNYQLKFSTDYILGGISNNI
                     LINRYQMYGGGNGPVQLNNGNDVNWSFRVGISDVMEDIKIVGGLRFGQSLSDKDAFIS
                     FQNFRKRLDWGVTYYRSVVSNFFPAGGYGNRLNTNLYQLNVSYPFDETKSLRATIGFR
                     SDRGIMKAEDHISLPFTDTITKYALARMEYVYDNTLNPAQNIWKGLRWKTYMDFNLPM
                     VKGASKNFNFNFGFDARNYLEIYKNIIWATRAAGDFSWGEQKIIYYLGGVDGWIGPKF
                     NNMNRPAPDQTYAFQSLAVNMRGFNQNIANGNNAIVINSEIRVPVFATFLNKPINNAF
                     LRNFQLVQFIDLGTAWNGKFNGIQRPYTIFPGSNSDDPVSVRIKAGGIGPFVGGYGFG
                     ARSTLLGYFLKADIAWEMKGIFKGKPIFYFAMGLDF"
     gene            6537..7694
                     /locus_tag="PLU36_04080"
     CDS             6537..7694
                     /locus_tag="PLU36_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMZ45959.1"
                     /translation="MSYLKFKANNIFSGTEMFSDEYVLIAKQDGTIEAIVKQSEAGDD
                     LQYFNGILSPGFINTHCHLELSHLKGIIREKTGLVNFILDIVANRNSKEDIILQAIKD
                     AEDTMLKNGIVAVGDICNTTYTLYQKKQNNLAYYNFIETSGWIPNIAEKKYNEAVNLY
                     NQFSPITKTCSIVPHAPYSVSNELWCLIEKHFQNKIISIHNQEANAENELFLNGTGAF
                     MYLYQQMNITNNSFQPTKKSSLQSYFHWLENTQNILLVHNTCTSEKDIHFLHQQPEFI
                     KHKLFFCLCPNANLYIEDTLPNINLLRESNIQLTLGTDSLASNNNLNIVNEIKTLQTH
                     YPKITLAEILQWATINGAKALDMEDTLGSFEKGKKPGIILLENTNDIKRLV"
     gene            complement(7763..8245)
                     /locus_tag="PLU36_04085"
     CDS             complement(7763..8245)
                     /locus_tag="PLU36_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45960.1"
                     /translation="MKKLNLLGTMLLGLLVLSTISCKKSTTSGSGSGSLTGSWSLVNA
                     KVEMLSGSTVVTSGTVTRAQIDADPVTYADLQFFKSLTFTSSTVTNGTGSTINYTIKT
                     GTDGNKYFVETGSADTDPDDRLYFYFEGGNLRIYNTQPGSPVAGSPTTQKVTMYYNKV
                     "
     gene            complement(8460..9065)
                     /gene="nusG"
                     /locus_tag="PLU36_04090"
     CDS             complement(8460..9065)
                     /gene="nusG"
                     /locus_tag="PLU36_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HMZ45961.1"
                     /translation="MMEEITVQETVQQQPAENTKWYVLRVVSGKERSVKEYLDKDIAR
                     NGWTTIVKQVFLPMEKVYKVQNGKKVMREKNYFPGYVMLEVIDGKLTDDLVQHISNIS
                     NIMHFLTDGKGSKGNIISLRKAEVNKMLGKVDEMNDLGVTISEPYIVGETIKIIEGPF
                     NDFNGVIEEVNDEKKKLKVVVKIFGRSTPVELNYVQVEKLN"
     gene            complement(9078..9272)
                     /gene="secE"
                     /locus_tag="PLU36_04095"
     CDS             complement(9078..9272)
                     /gene="secE"
                     /locus_tag="PLU36_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HMZ45962.1"
                     /translation="MNKISAYFKDSYKELVEKVTWPTWLQLQQSTVIVLVATLIITGL
                     VWIMDTASSQMLKLVYSLLN"
     gene            complement(9314..9384)
                     /locus_tag="PLU36_04100"
     tRNA            complement(9314..9384)
                     /locus_tag="PLU36_04100"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9350..9352),aa:Trp,seq:cca)
     gene            complement(9571..10758)
                     /gene="tuf"
                     /locus_tag="PLU36_04105"
     CDS             complement(9571..10758)
                     /gene="tuf"
                     /locus_tag="PLU36_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HMZ45963.1"
                     /translation="MSKETFKRDKPHVNIGTIGHVDHGKTTLTAAITDILSKQGLATA
                     KKYDEIDGAPEEKERGITINTAHVEYQTANRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTKEHILLAAQVGVPKMVVFLNKVDLVDDAELLDLVEMEVRDELSK
                     RGFDGDNTPIIKGSATGALAGEEKWVAAISELMAAVDEYIPLPPRPVDLPFLMSVEDV
                     FSITGRGTVATGRIERGVIKVGDAVEIVGLQEAPSTSTVTGVEMFKKLLDRGEAGDNA
                     GLLLRGIEKKDIRRGMVICAPKSITPHTEFKGEVYVLSKDEGGRHTPFFNKYRPQFYF
                     RTTDITGEVVLPEGTEMVMPGDNVSITVKLIAPIAMEKGLKFAIREGGRTVGAGQVTE
                     ILK"
BASE COUNT         3998 a         1727 c         1724 g         3580 t
ORIGIN      
        1 acacctacca acaatttgca acgacaattg aaaaactaac gcaaatagat tttggtcgtt
       61 tcgctaacat cacaaggaac tcagacacgg aaaagatagc ttcaaaaata ttgacatcaa
      121 tcaacaaaac aggacaacaa atatttattc atcacttccc gacattagcg aatattgatt
      181 ggaaaatcac aaataaaata atttcagaat taagtactga atatttgtat aatcttttaa
      241 gatgggagaa acttgacttg tatactgtca atttgccatc tttaaaatta gctcttgact
      301 attgtaaaaa gaccgaagaa tcaaaattta ttgaaggaat tataaataaa aataagtaca
      361 ggtttgacaa gtacagaaag caaaactaca agaagcactt gccataacat cacctatacg
      421 caagcagggg tttcgtgctt cgtaggacag aaaagtggta aatttaaggt tcagttcttc
      481 gtaggaagtt cagtggtgaa aatccctgcc tgcgtatagc tgagaaccgt tagaagcaat
      541 tttgctaaac cgtaccgata atgaaaactc aatttaaaac ctaaaaaaat attcaacata
      601 ggtttgtcta attaccaaat tttggtaact tcgaagaaag tatagaaaaa atgaaaaata
      661 catcaatatc actcggaaat tacttcgacc attttgtaag tagtcaagta tcagcaggac
      721 gttataaaaa tgtaagtgaa gtggttcgtg ctgggctaag acttttagag gacgaagaaa
      781 gtaaagttat tgctctgaag gatgcaattc aaaaaggtat tgacagtgga attgcgtacg
      841 attttgaccc acagaaacat cttcaagaaa taaaagcaaa aaaacggaat aatggcaaaa
      901 tatgaattga ccaatgaagc agttgaagat ttgactaaaa tttgggatta tacgattgat
      961 aagtggtctg agaaacaagc tgacagttat tatgaaatgt tacttaaaaa ctgccaacac
     1021 attgcggatg acccaaacat aggaagaaat tacgaaggaa tcagaaatga cctttttgga
     1081 ctgaaagtta accgacatat tattttttat cgaaagttaa gcaataaacc aattgagata
     1141 acaagaattt tacacgagcg aatggacttg aaaaacagat tgaatgaata aaaaaactgc
     1201 caccgacaaa caatttggca atagagccgg tgaagtactt ctatgaaaca tttgtgctaa
     1261 attcaacagc agtaattcta tataacttta gtgctcctat ttccaagccc tcgaaatgct
     1321 gccaacccgt tagctgtaag cgttttcgaa ctctcgtcca aaaatcaaca atataaaatt
     1381 tgcttgctgg aaacttttaa gttaactttg taaaatgaat actcaaagac aaatcatttt
     1441 tcacaaacac tatttcattg acttttatgt cgaacaaaat gaaaaggtgc aagaaaaaat
     1501 tgagtatgtt tttaagattt taaggaccgt tcagaaagtt cctaaaaagt ttctagacca
     1561 tatgactgga actgatggac tttatgaaat tagaatagaa tttgaaagta acatttacag
     1621 gattttttgt tgctttgaca aagataattt ggtcgttcta tttaatgggt ttcaaaagaa
     1681 gtctcaaaaa acacccaaaa aagaaattga tttagctctg aaattaaaag atgaatattt
     1741 taactcaaaa aaacaaagta atgaataaag aaaatatcaa aaatgctaaa aactttgatg
     1801 agttattaga cattaaatac ggaaagatag gctctgaaaa cagagacaaa tttgaagaaa
     1861 aagcacaata ttttgtaatt agtgaaatgt tgaaagaagc tcgtaaagaa gccaatatga
     1921 ctcaagaaca acttgctgaa aaagtaggga caaagaaaag ctatatttct cgacttgaaa
     1981 atggaaaatg tgatattcag ctttcaactc tctatagaat tttcgaattt ggacttggaa
     2041 aacgagtaaa cttgctattt ggataaaagt aacgcctaca gctaacgggc agttttgcaa
     2101 gagcaggggt gaagtgcaaa attcaacatt tgtacttctt ttcagcagta gtactaaatt
     2161 aaactttagg ctcctatttc caagccctcg aaatgctgcc aatccataag gtaaaatccc
     2221 attcattcgt attagtcaaa acgttgcttt acaactactc ttttgctaat ttgttggtaa
     2281 atgaattttg gtttcagaac agaaacctat tttaagttaa aagcttcttt acgagggtaa
     2341 aaaagaattt aaaaaaaggg attattatcc ttttcgtagc caatagttgt tgtgggacca
     2401 tgcccgctaa atactttggt attgttgggt aaggtatata atttttgttc aatactgctc
     2461 attaaagtat cataatctcc tccaggcaaa tctgttcttc caatactttc ataaaacaac
     2521 acatcaccta caatagcaaa gtttgctttt ctattgtaaa aagacagatg accaggacta
     2581 tggcctggtg taaaaagaat ggttaattct tcctcttcaa tttctatttt atcctcttcc
     2641 tttaaaaaat gaatattgcc tttatagttt tcaaaagaca aaccatattg ttggccacta
     2701 atgggtgcat attccaacat catttgctct tttggatgaa tgtataattc taatccgtaa
     2761 gtatcgtgta taaacttatt tccaaacaca tgatctaaat ggcagtgtgt attgatgagt
     2821 tgtattggaa ctaatttatt ttcttcaata aattgtttaa gtaagttctt ttcctcttca
     2881 aaaaaacatc ctggatcaaa tattacagct ttacgatttt ggttataaac aatgtatgta
     2941 ttttcttgaa taggattaaa tgtaaaactc ttaacagtta gcatatcatt ttttatagca
     3001 aattaatctc taattgctta attttagcaa gtattaaata atatttctgc taatttgccc
     3061 aactatttta ttgataatta agattaaatt ttttaaaaga aatggccaga acgaatttta
     3121 agcaattaca cttgtttttt ttgataggta ttttattttc ttatgcaaca atacaagctc
     3181 aggtaaatac agtagagttt ggtaaaaata gaattcagca taaaaaatta aaatggaaat
     3241 tttatcaatc taaaaatttt aatacctaca tggcacaagg tggtacagag attgccagat
     3301 ttgtagcaca agttgccgaa gaggaattac cagaactgga aaattttatt gaatacaacc
     3361 ttcatagaag agctgatatt gtagtgtaca caaattttga agattataaa caaagtaata
     3421 ttggattagg aattgattgg caacaatctg gtggtttaac caaattagta aacaataaaa
     3481 ttgttgtttt ttttgatggc aaccataata atttaaaagt aaaaataaga gaaggtattg
     3541 ctaaagtact aacagataat ttattgtttg gagatgatat tggtgaattt gcaagtaacc
     3601 aagcattgtt agatttgccc caatggctta cagatggcta cgtttcctat gcagcagaaa
     3661 attggagtac caaaaaagac gatgaattaa aaagtgaaat actaagtggc aactataaaa
     3721 atttttacaa ttttgcatat ttaaaaccta ttcttgcagg tcattctttt tggaatttta
     3781 ttgcagaaaa atacaaaaaa gataatgtaa cctatttttt gtatttggct agaatgtata
     3841 aaaacttaaa cacagcctcc ttaaaaatat gcaagaaaaa atttaaagat gtgcttgcag
     3901 agtttatgga gagcaagcaa aatcaatata cagaagacat cagaaaaaga aaaaatgcaa
     3961 ctaaaggtaa acgaaccata tcagaagatg ttagtaagca taattattat aattttcaaa
     4021 taaaccccaa ccctaaaaat aatgcctatg caatggtaca gtttaaaaaa ggtatgtata
     4081 gtgttatttt aaatgataat tattattatg taaaaacatt attaaaaaga ggtgttaggg
     4141 ttttacaagg agaccaaagc ccaaattatc ccataatggc ttgggatggc aaaggaacca
     4201 ggcttttagt aatttatgct gaagatgcta aagtaaaatt atttgtttgg gattatgtgg
     4261 cacgctacaa aagatttaaa caagttttag aaggtgttga tcaggttatt gatgcctcgt
     4321 ttatgttaga tgctaatact gttattttaa gtgctgtaaa aaatgggcat accgatattt
     4381 ttatttataa aattcaagaa aataaactag agcaaattac caacgatgtg tatgatgatt
     4441 taaacccaac ttatgtttct tttcctaatc gttcaggaat tattttctcc tccaacaggc
     4501 caagtgcaaa tgcccctatg gaagatacag caataccaag tagaaataga tttaacgttt
     4561 ttttagtgga tatacttaat aaaagtgcaa ctaaacaaat aactcaatta agcagtttac
     4621 aatacagcaa tgcatcttac ccaatgcagt ataatgtaaa ccattttacg tttgttgccg
     4681 atgaaacagg aattggcaat cgttatgcag gcttcttctc tacacaaaga gatggcttag
     4741 atacacttta ctatattgga gaggacatca ttagaaatcc aaccaaaaat gaattagata
     4801 gctcattagc tgcttggaga atagaaaagc ctgatagtgt aagctacttt caagtttata
     4861 aagattctac ttatactttt cctataacca attatcaaag ctctatttta gaaacaagaa
     4921 ttgcaggcaa taatggaatg gttagtgaag taagaagaga aggagattat aagtatttat
     4981 ttaaactgca agtagatgat aaagcattaa aaaatagaaa tattaaccct gctttatcta
     5041 actatatgaa gcaagtaaga ggaattgtaa aacaacaaaa aggcaaacca aataaatcta
     5101 aaaaagaaac aacaactgta aaggcagata aaccaactac tgatacaacc aaaccaacca
     5161 ataaaaaagt ttttcaaaca gagtttgatg atagtgttga aaacacagac actgcaaata
     5221 ctaccaataa aacaactcct gcaaatacag gtaataaaga atcggaaaaa attgagctta
     5281 agaaaagatt gtttaattat caattaaagt ttagtacaga ttatatttta ggaggcatta
     5341 gtaataatat attaattaac cgttatcaga tgtatggtgg aggtaatgga ccagttcaat
     5401 taaacaatgg taacgatgta aactggagtt ttagagttgg tataagcgat gtaatggaag
     5461 atattaaaat tgtgggagga ttaagatttg gacaatctct tagcgataaa gatgcattta
     5521 tttcatttca aaattttaga aaaagattag attggggagt aacttattac agaagtgtgg
     5581 tttctaattt ctttcctgct ggtggctatg gcaacaggtt aaatacaaac ttgtatcaac
     5641 taaatgtctc ttatcctttt gatgaaacca agagtttaag agcaactatt ggctttagat
     5701 cagacagagg aataatgaaa gctgaagatc acatttcttt accttttact gataccatta
     5761 caaaatatgc tctggcaaga atggagtatg tgtatgataa tactttaaac cctgctcaaa
     5821 atatttggaa aggtttaaga tggaaaacat atatggattt taacctgcca atggtaaaag
     5881 gagccagtaa aaattttaat ttcaattttg gttttgatgc cagaaactat ctagaaattt
     5941 ataaaaatat tatttgggct accagagctg caggtgattt tagctggggc gaacaaaaga
     6001 taatttatta tttaggtggt gtagatggat ggattggacc taagtttaat aatatgaaca
     6061 ggcctgcacc agaccaaacc tatgcttttc aaagcttagc agttaacatg cgtggcttta
     6121 atcaaaatat tgctaatggt aataatgcaa ttgtaattaa tagtgaaatt agagtgcctg
     6181 tttttgcaac atttttgaac aaacctatta ataatgcttt tttacgcaac tttcagttag
     6241 tacaatttat agatttaggc actgcatgga atggcaaatt taatggcatt caaaggcctt
     6301 ataccatttt ccctggtagc aattccgatg atccagtttc tgtaagaatt aaagctggtg
     6361 gcattggtcc atttgttggt ggttatggtt ttggagcaag aagtacacta ctgggttatt
     6421 ttttaaaagc tgatattgca tgggaaatga aagggatttt taaaggaaag cctattttct
     6481 attttgcaat ggggttagat ttttaatacc caattattgg attaattaat ttaaaattga
     6541 gctacttaaa gtttaaggca aacaatattt tttctggaac agaaatgttt tcagatgaat
     6601 atgttttaat agccaaacaa gatggaacta ttgaagctat tgtaaaacaa tcagaggctg
     6661 gagatgattt acaatatttt aatggaatat tatctcccgg atttattaat acccattgcc
     6721 atcttgagtt aagccactta aaaggtatta ttagagaaaa aacaggctta gtaaatttta
     6781 tattagacat tgtagcaaat agaaacagca aggaagatat aattttacaa gcaataaaag
     6841 atgctgaaga taccatgcta aaaaatggta ttgttgcagt tggagatatt tgtaatacta
     6901 cttatacact ttatcaaaaa aaacaaaata atttagccta ctataacttt attgaaacaa
     6961 gtggatggat ccctaatata gcagaaaaaa aatacaacga ggcagttaat ttgtacaacc
     7021 aattttctcc aataactaaa acatgttcta ttgttccgca tgctccatat tcagtatcca
     7081 atgaattatg gtgtttaata gaaaaacatt ttcaaaataa aataatttca attcacaatc
     7141 aagaggcaaa tgcagagaat gagttattcc ttaatggcac aggggctttt atgtatttgt
     7201 atcaacaaat gaatattaca aataattcat ttcaacccac aaaaaaaagt agtttgcaat
     7261 cttactttca ttggctggaa aacactcaga atattttatt ggtacacaat acatgcactt
     7321 ctgaaaaaga cattcatttt ttgcatcaac aaccagagtt tataaaacat aaactcttct
     7381 tttgtttgtg tcctaatgcc aatttatata tagaagatac tttacccaat attaatttat
     7441 taagagaaag taacatacaa cttactttag gtacagatag tttagctagt aataacaatt
     7501 taaatatagt taatgaaata aaaactttac aaacacatta tcccaaaatt acattagcag
     7561 aaatactaca atgggctacc attaatggag caaaggcttt agatatggaa gatacattgg
     7621 gaagttttga aaaaggcaaa aaaccaggta ttattttatt agaaaatact aatgatataa
     7681 aaagattagt gtagtttaaa caaaaaacga ttgcctttta atttaaggca atcgtttttt
     7741 aaagtataat gaataaaata aattacactt tgttatagta catggttact ttttgagttg
     7801 ttgggcttcc tgcaactggt gatcctggtt gtgtgttata gatacgaaga ttacctcctt
     7861 caaaataaaa atacaaacga tcatctgggt ctgtatctgc acttcctgtt tctacgaaat
     7921 atttattccc atctgtacct gtttttattg tataattaat agttgaacca gtaccattag
     7981 taacagtgct acttgtaaaa gtaagtgatt tgaaaaattg taaatcagca taagttacag
     8041 ggtcagcatc tatctgtgct ctagtaactg tacctgaagt taccactgta gaacctgata
     8101 acatttctac ttttgcattt actaaactcc atgaacctgt taatgagcca gaaccactac
     8161 cacttgtagt actttttttg caagaaatag tacttaaaac taataagcca agtagcattg
     8221 tacctaataa atttaatttt ttcatattta attttttatg tgataaaaaa caaatatact
     8281 tattttttta tattcatgca tgagtttatc ttttaattaa cttaaatctt tactatcaat
     8341 accttctaat tttaattata acataaagcc tttttttaga agtaactaaa taaaaagaat
     8401 gttcagacaa taaaaaaagg agccataagc tcctttcgta aaatttaatt gtattatttt
     8461 tagttcaatt tttctacttg aacataattt aactccactg gtgtgcttct tccaaatatt
     8521 ttaactacta cttttaattt tttcttttca tcattcactt cttcaataac accattaaaa
     8581 tcgttgaatg gaccttcaat aattttaatt gtttcgccaa caatatatgg ttcgctgata
     8641 gttacaccta aatcattcat ttcatcaacc ttaccaagca ttttattaac ctctgcttta
     8701 cgtaaagaaa taatgtttcc tttagatcct ttaccatcag ttaaaaagtg catgatgtta
     8761 gaaatattac ttatatgctg tactaaatca tcagtcagct ttccatcaat tacttcaagc
     8821 attacataac ctggaaaata gtttttttcc ctcattactt ttttaccatt ttgtacttta
     8881 tatacttttt ccattggtaa aaaaacctgt ttcacaattg tagtccaccc attacgagct
     8941 atatctttat ccaagtattc ttttacgcta cgttcttttc cactaacaac tctcaacaca
     9001 taccatttag tattttctgc tggttgttgt tgtacggttt cctgtacagt tatttcttcc
     9061 atcattaatt caaattatta atttaataaa gaataaacta gctttaacat ttggctgctg
     9121 gcagtatcca ttatccaaac taaacctgta ataattaaag tagctaccaa cactattaca
     9181 gtactttgtt gtaattgtaa ccaagttggc caggtaactt tttctaccaa ttctttataa
     9241 ctgtctttaa aataagccga aattttattc atctttaaac tatttagttt tataaaaaat
     9301 taaataatag ttgcacgggc acaaggattc gaaccctgat caaaggtttt ggagacctct
     9361 attctaccgt tgaactatgc ccgtattata ctaatatttc tactagtaat ttttaaaatg
     9421 ctcaaaaaac attttaaaat atgtttaaga actgtgcctt ttaagaaagc taaaagctat
     9481 tagcaggttg ccaatagctt ttagcatatt tatcaaagat aggtttttta agcaattaag
     9541 cttaaggtaa ggctaaaagc taaccctatc ttattttaaa atttcagtta cttgtcctgc
     9601 acctactgtt ctaccgcctt cacgaatagc aaattttaaa cctttttcca ttgctatagg
     9661 agcaataagt ttaacagtaa tgcttacatt atcaccaggc ataaccattt cagtaccttc
     9721 tggtaaaact acttctccag taatatcagt agtacggaaa tagaattgag gacgatattt
     9781 attaaagaaa ggagtatgac gaccaccttc atctttactt aacacataca cttcaccttt
     9841 aaattcagta tgcggagtaa ttgatttagg agcacaaatt accatacctc ttctaatatc
     9901 ttttttctca ataccacgta ataataaacc agcattatca ccagcttcgc cacggtctaa
     9961 taatttttta aacatttcaa caccagtaac tgtagatgta gaaggagctt cttgtaagcc
    10021 tactatttca acagcatcgc ctactttaat aacacctctt tcaattctac cagtagcaac
    10081 tgtaccacga ccagtaatag agaaaacgtc ttctacactc atcaaaaatg gtaaatctac
    10141 cggacgagga ggtaaaggaa tatattcatc tacagcagcc attaattcac taattgcagc
    10201 aacccatttt tcttcgccag ctaatgcacc agttgcagaa cctttaatga ttggagtatt
    10261 gtctccatca aatcctcttt tagataattc atctcttact tccatttcta ctaagtctaa
    10321 caactcagca tcatctacta aatctacttt atttaagaaa actaccattt taggtacacc
    10381 aacctgtgca gccaaaagaa tatgttcttt ggtttgaggc ataggaccat ctgtagcagc
    10441 aaccactaaa attgctccat ccatttgagc agcaccagta atcatgtttt ttacatagtc
    10501 agcgtgacca ggacagtcaa cgtgagcata gtgacgattt gctgtttgat attctacgtg
    10561 agctgtattg atagtgatac cacgctcttt ttcttctggt gcaccatcaa tttcatcata
    10621 ctttttagcc gttgccagac cttgcttaga tagaatgtct gtaatggcag cagttaaagt
    10681 tgttttacca tggtcaacgt ggccaatggt accaatgttt acgtggggtt tgtccctctt
    10741 aaaggtctct ttagacattt ttatcgattt ttagttgtgt taaaaattat agcttattaa
    10801 aatttttaaa tttattttta tttaaccagc aacattatca gctatttaac ttctgttgcg
    10861 tcgcactctt caacaccttt ctatttatta agcaataaaa aaccacttca aaaattaaaa
    10921 tcaactaatt gcacaagttt agtacttatt ttaaaatagt aaacttacac gacattgagc
    10981 cgttgatgag aattgaactc acgacctctt ccttaccaag gaagtgctc
//
LOCUS       DAOQPK010000054        10894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_3120_length_10894_cov_5.025740, whole genome shotgun sequence.
ACCESSION   DAOQPK010000054 DAOQPK010000000
VERSION     DAOQPK010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10894
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3120_length_10894_cov_5.025740"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1915)
                     /locus_tag="PLU36_04110"
     CDS             complement(<1..1915)
                     /locus_tag="PLU36_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HMZ45964.1"
                     /translation="MKKLKIPRYIRWIAQTGIMFLILMSLMRWVLVLTYRTPTEKSVS
                     LIDAYWLGFRFDLRFVSITMLLLYLVGTIPYLHPINKKWGNRVSFIIWSVFIVLTSIF
                     YAADFAHYSYLRQRLNANILSFLHDAKISFGMMWQSYNLGWGILGLIVIVFILLAMVK
                     LSYNHILSKANVSTKKSSKVWGVLFVLLLAFGIVGNVVYKAGQYPLRWSNAYTLGNDY
                     AANISLNPFQSFFSTLKFKDVTFDEEKVKKHYEWMSAYLDVPLNQRDSNKLNFNRIQE
                     LDSSNKMAQHIQNVVLVICESFSAYKSSMYGNPLNTTPFFAEMCKEGVFFNRAFSPAY
                     GTARGVWAIVTGIPDVQFNKTSSRNPATVNQHTIINDFNQHEKFYFLGGSSSWANIRG
                     LLTNNIKDLRLYEQGSYSSKEIDVWGISDKNLFLEANKTLAQQTKPFFAVIQTADNHR
                     PYTIPKEDLKEFKIQKIKNAQLKKYGFESEEEYNAFRYTDFSFQKFIEAAKKEKYFNN
                     TLFVFIGDHGIRGDAGDMLPKAFTTQGLTNMHVPLLFYAPGILPAKEITFPASQLDVL
                     PSIAYLCNIPYTNTTMGRNLFKLKNTNDNYTFIIDEGAKIIGALNDNYFFNYQLKDKK
                     VENFVSLQHNSPVT"
     gene            1984..2580
                     /locus_tag="PLU36_04115"
     CDS             1984..2580
                     /locus_tag="PLU36_04115"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HMZ45965.1"
                     /translation="MIFVFLNSFFEWLDKADKQLFLLVNKEWTNTFLDNNLPWYRDST
                     TWLPLYLFLLLFVIFNYGKKSLPWIIAVAITSTISDQISSNFLKNLIERPRPCNDVFI
                     QHLGRLLLNRCPSSFSFTSSHAVNHFTAAMFIFTTLKPAFGKYATLFPIWAATICYAQ
                     VYVGVHYPLDVFAGAIAGCCIGIFAGKWYNKYYSLQTI"
     gene            complement(2600..2944)
                     /locus_tag="PLU36_04120"
     CDS             complement(2600..2944)
                     /locus_tag="PLU36_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HMZ45966.1"
                     /translation="MSSLVQEFNNYRSKMNDVILSKNNLVMKRLWNLDTNTYEDGALD
                     KKTKEMLGLVASMVLRCDDCIKYHVQKCFELGITTEQLYEIFAVANIVGGTIVIPHTR
                     RAAEYWEELIKQ"
     gene            complement(2972..3379)
                     /locus_tag="PLU36_04125"
     CDS             complement(2972..3379)
                     /locus_tag="PLU36_04125"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HMZ45967.1"
                     /translation="MKVLVITVGFIFCCFQLNAQAKGQSSKVDLDTIYNKVDVLPSYI
                     GGEEGWKKYLKKNTKYPKKAWWEELEADVTLEFTIKNDGSIVDIINLTVSGWGFEEEA
                     IRLLKKSGKWNPALKNGKPVHYHAKLTIPFRLK"
     gene            3445..6600
                     /locus_tag="PLU36_04130"
     CDS             3445..6600
                     /locus_tag="PLU36_04130"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HMZ45968.1"
                     /translation="MKIIINLLMGKDTINKNFNDAYKSLNQEQKKAVDTIEGPILVNA
                     GPGTGKTQILATRIGNILLNADVLPNNILCLTYTDNGAVEMRKRLLSIIGSAAYNIQI
                     HTFHSFCNEVIQDNVTYFGKLNLEPISELEEIELFNKIIDTIPNNSALKRFSGDVYYE
                     KRRLQGLFALMKKEAWQPEHLLHCIDSYITELPTKEGFYYKRKYKQFNAGDAKIGAIN
                     AEIERMEILKEAVKLFPIYNKMMNENSRYNFDDMILWVLQAFNENNNLLLNYQERFLY
                     ILVDEFQDTSRSQNLLLQHLISYWDVPNVFVVGDADQSIFSFQDANVQNIESFINQYK
                     QHIVEIDLTTNYRSTQNILNTAYSLIKNNALRITKTLQPLEASDENRQKEDIVPTILE
                     FNTPAHEAIYIAKQIEELIKNKNVNGKDIAVIYRKHDIANEIANILQQKNIPINTKKK
                     VNILQLPFINNIIKILQWIDKENYLPYSGDDILFKILHSNFWNLKSLDIAKISVAVNN
                     ANKASKADKFSLRRWLNEMETKTADLFNQQKDELKSVINALESLLKESNNCTVQQLFE
                     KVIQTCGILSFIMQSNEKPWLMQVLTSLFNLIKEETKRNSDLTLSDLVKYLALMEKHN
                     ISPTVQKVINNTNGVNFITAHSSKGSEFEYVFVIGCTENVWEKGGGNNKVYKLPDNLV
                     HNNSIATNNEESRRLFYVAITRAKTHLQLSYFKYDVKDKAQVESSFIAEICNNSNITI
                     KPTEVSEDDFVAYTLLQFSESAIPEIELINNEYLDKILQNYTLSVTHLNNYLSCPIKF
                     YYQNLIKVPSAKNASMTFGTAVHYALETLFKNMIGNNNQFPSVQEFVDYFIYSMKKNK
                     EAFTAEEYKLKTDYGYKILPAYYNYHINNWQKIVKIEATFKNILVQNIPINGKLDKLE
                     FNGYNVNVVDYKTGKFANAKEKLIAPCDKEPNGGDYWRQAVFYKILIDNDKTNNWQVK
                     STQFEFVEPEKEKYEIERIDIKEEDVTTVKQQIIEVWNKIQAKEFNKGCGKPTCEWCN
                     FVKTNELAVALHTLIEEDETL"
     gene            6661..8067
                     /locus_tag="PLU36_04135"
     CDS             6661..8067
                     /locus_tag="PLU36_04135"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HMZ45969.1"
                     /translation="MNKKTSLLLLALLFITINFLLISCANIIPPGGGPRDSLPPQLMQ
                     ALPKNFSTQFSSNKIVLSFNEYIELKEITKSLIVSPLPKNQPSVDYKLKTITIKLKDS
                     LIPNTTYSINFGNSIVDINEGNPLSNFTYVYSTGNTIDSCQLKGKVILAENGKIDSTL
                     IAVLYSNLSDTAVITQKPLYITQLKGDGSFTFKHIPPGIYAVYAMPNDYAKRYNDSTK
                     LFAFTDSAINVNYSNINSTTLYAFQEAKNNQSTPVASNNNIKNNNKAKHLKYTSTVEF
                     EKQDILNSTLELLFTSSINKLNTSKILLTDTNFNAYNNYTTALDSVSNKIILTTSWLA
                     NTAYKLLIYKDAVYNKQGNTIAQNDTLSFLTKKETDYGALKLRFNNLDTLKYPVLLIY
                     QQTNLVEAIKINKTIISRKLFKPGDFDLKILYDKNNNGIWNTGNYKNKLQPEIVVPLD
                     KKLSIRANWDNEAEINIK"
     gene            complement(8081..8254)
                     /locus_tag="PLU36_04140"
     CDS             complement(8081..8254)
                     /locus_tag="PLU36_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45970.1"
                     /translation="MSHSHFEGDSKKFTLTAILSFAIVFCFLALFSRCNGDYKTDKSA
                     GSHTPATKEHKAH"
     gene            8413..9156
                     /gene="ubiE"
                     /locus_tag="PLU36_04145"
     CDS             8413..9156
                     /gene="ubiE"
                     /locus_tag="PLU36_04145"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HMZ45971.1"
                     /translation="MTKFAHDAIVPDTNSQLTKKQQVAAMFNDIAFKYDFLNRFLSAG
                     IDIRWRKKGIKQLKELKPQFVLDVATGTADVALLTYKLLQPKKIIGIDISEGMLKIGK
                     QKIADKKLQQIIELEIGDSEQLNFTENSFDAITVAFGVRNFEHLEKGLENMLKVLKPG
                     GKLVIMECTQPKQTALKGFYKFYMNFITPGIGKMIAKNKQAYQYLNNSIQAFPEGETF
                     LNIMNKAGYKNTYLKTLSLGLCTIYCGSK"
     gene            9129..9842
                     /locus_tag="PLU36_04150"
     CDS             9129..9842
                     /locus_tag="PLU36_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMZ45972.1"
                     /translation="MYYLLRKQIILFGIFIFVITNVFAQKYVELNRPDRDELPYYFGM
                     TLAVNQSYLAATKHTKFINDDSILSVQPGASSGISLGLLGTLKLNYCWEFRFNPQLII
                     GGSKHFIYQKNYPVEEVKKTLPSTIVSFPFQFKLNSDRLNNFRTYVLFGAKYDIDLAS
                     NSSARNAQDMVKLKKSDFALEAGIGFNFFLPFVTISPELKFSYGLSNIHSRDPALKYS
                     NVFDKIQSRMISLSIHLED"
     gene            9958..10110
                     /locus_tag="PLU36_04155"
     CDS             9958..10110
                     /locus_tag="PLU36_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45973.1"
                     /translation="MKELIEKLSTEVGLTPEQANKAIEAIASFVKEKFPMLGGAVDSI
                     FSKEEE"
     gene            10586..>10894
                     /locus_tag="PLU36_04160"
     CDS             10586..>10894
                     /locus_tag="PLU36_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HMZ45974.1"
                     /translation="MEIISNLGRDNKKRRKSNIDLSTMVYGKVPPQARELEEAVLGAI
                     MLEKGAFDKVVDVNLKSECFYVEAHQHIYKAMQDMQQQSIPIDILTVVEELKRKELLQ
                     Q"
BASE COUNT         4145 a         1586 c         1538 g         3625 t
ORIGIN      
        1 ttgtaactgg tgaattgtgt tgaaggctaa caaaattttc tactttttta tcttttagct
       61 gataattaaa aaagtaatta tcgtttaatg ctccaataat ttttgcacct tcatcaatta
      121 taaaagtata attatcgttt gtatttttta gtttaaataa gtttcttccc attgtagtat
      181 ttgtatatgg tatattacat aaataagcaa tagaaggcaa cacatctaat tgcgatgctg
      241 gaaaagttat ttcttttgca ggtaaaatgc ctggtgcata aaataacaaa ggcacatgca
      301 tattggttaa gccttgtgtg gtaaaggctt ttggcaacat atctcctgca tctcctctaa
      361 taccatggtc tccaataaaa acaaataaag tgttattaaa gtatttttct tttttagcag
      421 cctctataaa tttttgaaaa ctaaaatctg tatatctaaa agcattgtat tcctcttcgc
      481 tttcaaaacc atattttttt agttgggcat ttttaatttt ttgaatttta aattctttta
      541 aatcttcttt aggtatggtg tatggtctat gattatcggc agtttgaata actgcaaaaa
      601 atggttttgt ttgttgtgca agagttttat tagcttctaa aaaaaggttt ttatcactaa
      661 tgccccaaac atcaatttct tttgatgagt aactaccttg ttcatacaac ctcaggtctt
      721 ttatattatt tgttaatagc cctctaatat ttgcccaact actactacct cctaaaaaat
      781 aaaatttttc gtgttggtta aaatcattaa taatagtgtg ttgatttaca gttgcagggt
      841 tacggctaga tgttttatta aactgtacat ctggtatgcc tgtaacaatt gcccaaacac
      901 ctctagcagt gccataagca ggagaaaaag ctctgttaaa aaaaacacct tctttacaca
      961 tttctgcaaa aaatggcgta gtatttaaag ggttgccata catactactt ttataagcag
     1021 aaaaagattc gcaaataacc aatacaacat tttgtatatg ttgtgccatt ttattactgc
     1081 tatccaattc ttgaattcta ttaaagttta atttattact gtccctttga tttaaaggaa
     1141 catctaaata agcactcatc cattcataat gcttttttac tttctcttca tcaaaagtta
     1201 cgtctttaaa ttttagggta ctaaaaaaag attgaaaggg attgagagaa atatttgcag
     1261 cataatcgtt gcccaaagta taagcattac tccaacgtaa agggtattgt cctgccttgt
     1321 aaactacatt gccaacaatg ccaaaagcca atagcagcac aaaaagaaca ccccatactt
     1381 tactactttt tttagttgaa acattagcct tacttaaaat atggttataa ctaagtttta
     1441 ccatagctaa taaaataaat acaataacta ttagccctaa aatgccccaa cctaaattgt
     1501 agctctgcca catcattcca aaagatattt tggcatcgtg tagaaagctt aaaatatttg
     1561 cattaagcct ttggcgtaaa taagaataat gtgcaaaatc tgcagcataa aaaatactgg
     1621 taagtacaat aaaaaccgac caaataataa acgaaactct attgccccat tttttgttta
     1681 taggatgtaa ataaggtatt gtaccaacta agtacaataa taacatggtt atggatacaa
     1741 accttaagtc gaacctaaac ccaagccaat aagcatctat taaacttaca gatttttctg
     1801 ttggcgttct ataagttagt acaagtaccc aacgcattaa gctcattaaa attaaaaaca
     1861 taatgcctgt ttgagctatc catcttatat atctgggaat ttttagtttt ttcattcaac
     1921 aacaaaagta taaaataaaa acattgcttt tattccattc attaagcaat tatctttaca
     1981 gcaatgatat tcgttttctt aaatagtttt tttgaatggc ttgataaggc agataagcaa
     2041 ctgttcttat tagttaataa agaatggact aatacctttt tagataataa tttaccttgg
     2101 tatagagatt ctacaacatg gctaccgctg tacttatttt tattactatt tgtaattttt
     2161 aattacggaa aaaaatcgtt gccctggata attgctgtgg caataacttc caccatctct
     2221 gatcaaataa gtagtaattt tttaaaaaac ttaatagaac gcccaaggcc ttgtaatgat
     2281 gtgtttatac aacaccttgg caggctttta ttaaatcgtt gtccatctag tttcagtttt
     2341 acatcctctc atgctgtaaa ccattttact gcagctatgt ttatatttac aacactaaag
     2401 ccagcatttg gaaaatatgc tactcttttt ccaatttggg cagcaactat ttgctatgca
     2461 caagtgtatg ttggggttca ttatccttta gatgtttttg ctggtgcaat agcaggttgt
     2521 tgtattggta tttttgcagg aaaatggtat aataaatatt acagtttaca aactatataa
     2581 tttttcaaat acaaattgtt tactgtttta ttaattcttc ccaatattca gcagctcttc
     2641 tagtatgtgg tataacaatt gttcctccaa caatatttgc tacagcaaaa atttcgtaca
     2701 attgttcggt tgtaattcct aattcaaaac atttttgaac atggtatttt atacaatcat
     2761 cgcatcttaa aaccatgctt gcaactaacc caagcatttc ttttgttttt ttatccaatg
     2821 ctccatcttc ataagtattc gtatctaaat tccataaacg tttcattact aaattattct
     2881 tacttagtat tacatcattc attttagaac gatagttgtt aaactcctgc actaaactgc
     2941 tcatatatat gtgttgaaaa attaaacaaa attattttag tctaaaagga attgtaagtt
     3001 ttgcatgata gtgaacaggc ttaccatttt ttaaggcagg gttccatttt cctgattttt
     3061 ttaacaaacg aatagcctcc tcttcaaaac cccagcctga tacagttaaa ttaattatat
     3121 ctacaatact gccatcgttt ttaatagtaa actctagtgt tacatcagct tctaactctt
     3181 cccaccaagc ttttttgggg tactttgtat ttttctttaa atattttttc cacccttctt
     3241 ctccacctat ataactaggt aaaacatcaa ctttattata aatggtatct agatctactt
     3301 tagatgattg cccttttgct tgtgcattta attgaaagca acaaaaaata aatccaactg
     3361 ttattactaa tactttcata aacaaaataa aatttcaatt aataacgtaa accaattaaa
     3421 aatcttacct acaaataagt aaatttgaaa ataataatta atttattaat gggtaaagat
     3481 acaataaata aaaactttaa cgatgcgtat aaaagtttaa accaagagca gaaaaaagct
     3541 gtggatacaa ttgaaggccc tatattagta aatgcaggac caggtacagg taaaacacaa
     3601 atactagcta cacgtattgg taatatttta ttaaatgccg atgttttgcc taataatatt
     3661 ttatgcttaa cctatacaga taatggagca gtggaaatgc gtaaaagatt attaagcatt
     3721 attggtagtg ctgcgtacaa cattcaaatt catactttcc atagtttttg caacgaagta
     3781 attcaagata atgtaaccta ttttggaaaa ttaaatttag agccaattag tgaattagaa
     3841 gaaatagaat tatttaataa aataattgat accataccta ataacagtgc tttaaaaaga
     3901 tttagtggcg atgtatatta cgaaaaaaga aggcttcaag ggctatttgc tttaatgaaa
     3961 aaagaagcat ggcagccaga gcatttattg cattgtattg atagctatat aactgaatta
     4021 ccaactaaag aaggttttta ttataaacga aaatataaac agtttaatgc aggcgatgca
     4081 aaaattggtg ctattaatgc cgagatagaa agaatggaaa ttttgaagga agctgtaaag
     4141 ttatttccta tatataataa aatgatgaat gaaaatagca ggtacaattt tgatgatatg
     4201 attttatggg tgctgcaagc atttaatgaa aataataatt tattattaaa ctatcaggaa
     4261 aggtttttat atattttggt ggatgaattt caagatacaa gccgttctca aaatttatta
     4321 ttacaacatt taattagtta ctgggatgtt ccaaatgtat ttgtagtagg agatgcagac
     4381 cagagtattt tctcctttca agatgccaat gttcaaaata tagaatcttt tattaaccaa
     4441 tacaaacaac atattgtaga aattgattta accaccaact atcgttcaac acaaaatatt
     4501 ttaaacacag cctattctct tattaaaaac aatgctctaa gaattacaaa aacacttcaa
     4561 cccttagaag catctgatga aaataggcaa aaagaagata ttgtacctac tattttagaa
     4621 tttaatacac ctgcacacga agcaatttat atagctaaac aaattgaaga attaataaaa
     4681 aataaaaatg ttaatggaaa ggatattgca gttatttata gaaaacatga tattgcaaat
     4741 gaaattgcta atattcttca acaaaaaaat ataccaatta acactaagaa aaaggtaaat
     4801 attttacaac taccttttat aaataatatc attaaaatat tacaatggat agataaagaa
     4861 aattatttac cctatagtgg agatgatatt ttatttaaaa ttttacacag taatttctgg
     4921 aatttaaaaa gcttagatat tgccaaaata agtgttgcag ttaataatgc caataaagca
     4981 agtaaagcag acaaattttc tttgcgtagg tggttaaatg agatggaaac aaaaactgcc
     5041 gatttattta atcaacaaaa agatgaactt aaatcagtaa ttaatgcttt agaaagcctt
     5101 cttaaagaaa gtaataattg cacagttcaa caactatttg aaaaagtaat tcaaacctgt
     5161 ggaatactta gttttattat gcaaagcaac gaaaagcctt ggctaatgca ggttttaacc
     5221 tctttgttta atttaataaa ggaagaaacc aaaagaaatt cagatttaac cctttctgac
     5281 cttgtgaagt acttagcact gatggaaaaa cataatatta gtccaactgt tcaaaaagtt
     5341 atcaataata ccaatggagt aaattttata acagcacaca gtagtaaagg aagtgagttt
     5401 gagtatgttt ttgtaattgg ttgcactgaa aatgtttggg aaaaaggtgg tggcaataat
     5461 aaagtataca aattacccga taatttagta cacaacaaca gcatagcaac aaacaatgaa
     5521 gaaagcagaa gactatttta tgtagcaata actagggcaa aaacacattt acaattaagt
     5581 tattttaagt atgatgtaaa agataaggca caagtagaaa gtagttttat tgcagaaatt
     5641 tgtaacaact ccaatatcac aattaagcct acagaggtta gcgaagacga ctttgtagcc
     5701 tatactttat tacaattctc ggaaagtgca atacccgaaa ttgaattaat aaacaacgaa
     5761 tatttagata aaattcttca aaattatact ttaagtgtta cccatttaaa caattattta
     5821 agctgcccaa ttaagtttta ttatcaaaac ttaattaaag tgccctctgc taaaaatgca
     5881 agtatgacat ttggtactgc agtgcattat gcactggaaa ctttgtttaa aaatatgatt
     5941 ggcaataaca accaatttcc atcggtacaa gagtttgtgg attattttat ttatagtatg
     6001 aaaaaaaaca aagaagcatt tacagcagaa gagtataaat taaaaacaga ttacggctat
     6061 aaaattttac ctgcttacta caattaccat atcaataact ggcaaaaaat tgtaaagata
     6121 gaagcaactt ttaaaaatat actagtgcaa aatattccta ttaacggaaa gttagacaaa
     6181 ttagaattta atggttataa tgtaaatgta gtagattata aaaccggaaa gtttgcaaat
     6241 gccaaagaaa aattaattgc accttgcgat aaagaaccaa atggaggaga ttattggagg
     6301 caagcagttt tttataagat tttaatagat aatgataaaa caaacaattg gcaagtaaag
     6361 agtactcagt ttgaatttgt agaaccagaa aaagaaaaat atgaaataga aaggatagat
     6421 ataaaagaag aagatgtaac aactgtaaaa caacaaataa tagaagtatg gaataaaatt
     6481 caggcaaaag aatttaataa aggttgtggt aagcctactt gcgaatggtg taattttgta
     6541 aaaacaaatg agctagctgt ggctttacat actttaatag aagaggatga aacattatga
     6601 tttttattgc atccatacct attaagtttg caaaatatta attgttgtag cattttatac
     6661 atgaataaaa aaacttctct ccttctatta gcattgttat tcattactat caacttttta
     6721 ttaattagtt gtgctaatat tattcctcca ggtggtggac caagagattc tttacctcct
     6781 caactaatgc aggctcttcc aaaaaatttc tctacacaat ttagtagcaa taaaatagtt
     6841 ttatctttta atgaatatat tgaactgaaa gaaataacaa aatctttaat agtttctcct
     6901 ttacccaaaa atcaaccaag tgttgattat aaactaaaaa caataacgat aaaactaaaa
     6961 gatagcttaa tacctaacac cacatattcc ataaattttg gtaacagtat tgttgatatt
     7021 aatgagggta accctttaag taattttact tatgtttact caacaggtaa tacaattgat
     7081 agctgccaac taaaaggtaa agttatttta gcagaaaatg gtaaaatcga ttctacctta
     7141 attgctgttt tatatagcaa cctttcagat acagcagtaa ttactcaaaa gccactttac
     7201 atcactcagt taaaaggaga tggtagtttt acttttaaac atataccacc aggtatatat
     7261 gcagtttatg caatgccaaa cgattatgca aaaaggtaca acgatagtac aaaacttttt
     7321 gcctttaccg atagtgcaat aaatgtaaac tatagcaata ttaattctac aacattatat
     7381 gcttttcaag aagcaaaaaa taatcaaagc acaccagttg caagtaataa taacataaaa
     7441 aataacaata aggctaaaca cttaaaatat acttctactg ttgaatttga aaaacaagat
     7501 attttaaatt caaccctaga gttacttttt acaagctcta ttaataaatt aaatacttct
     7561 aaaattttat taaccgatac taactttaat gcttacaata actacactac tgcgttagat
     7621 tcagtaagca ataaaataat tttaacaaca tcctggttgg caaatactgc ttataaatta
     7681 ttaatttata aagatgctgt ttataacaaa caaggcaata caattgcaca aaatgacaca
     7741 ttaagttttt taacaaaaaa agaaactgat tatggtgctt taaaattaag gtttaataat
     7801 ttagacactt taaagtaccc agtgttatta atttaccaac aaactaacct ggtagaagct
     7861 attaaaataa ataaaactat tatcagcaga aaattattta agccaggcga ttttgattta
     7921 aaaattttat acgataaaaa taataatgga atttggaata ccggaaatta taaaaataaa
     7981 ctacaacctg aaatagtagt tcctttagat aaaaaactat ccattcgtgc caattgggac
     8041 aatgaagcag aaattaatat aaaataatca ataattactt ttaatgagct ttatgctctt
     8101 ttgttgcagg tgtatgactt ccagcagatt tatcagtttt ataatcgcca ttacaacggc
     8161 taaataatgc taaaaaacaa aacacaatag caaaagataa aattgctgtt aaagtaaatt
     8221 tcttactatc tccttcaaaa tgtgagtgcg acataatgtt gtttttttcg ccagcaagat
     8281 atacaaattt tttaaactat acttttttgc ataatatttt aatttataaa tctaaaacca
     8341 cttttacata aaatttttgt tttgttaata cctgcccagc tttatgctca atccttatct
     8401 tcgcaaataa ctatgacaaa gtttgcacat gatgctatag tgccagatac aaacagccag
     8461 ttgactaaaa aacaacaggt tgctgctatg tttaatgata ttgcttttaa gtacgacttc
     8521 ttaaataggt ttttaagtgc aggaattgat attagatgga gaaaaaaagg tattaaacaa
     8581 ctaaaagaat taaagccaca atttgtttta gatgtagcta caggcactgc agatgttgca
     8641 ttacttacct acaaattact tcaaccaaaa aaaattattg gtatagatat tagcgaaggc
     8701 atgttaaaaa ttggcaaaca aaaaattgcc gataaaaaac ttcaacaaat tattgaacta
     8761 gaaataggcg atagcgaaca attaaatttt actgaaaaca gttttgatgc tattactgtt
     8821 gcttttggtg taagaaattt tgaacattta gaaaaaggat tagaaaacat gttaaaagtt
     8881 ttaaaaccag gcggcaaatt agtaattatg gagtgtaccc aaccaaaaca aacagcatta
     8941 aagggttttt ataaatttta tatgaacttt ataacaccgg gaattggtaa aatgattgca
     9001 aaaaacaaac aagcttacca gtatcttaat aattcaattc aggcttttcc agaaggtgaa
     9061 acttttttga atattatgaa taaggcaggt tataaaaata cttatttaaa aacattgagt
     9121 ttaggattat gtactattta ctgcggaagc aaataatatt atttggcata tttatttttg
     9181 taataacaaa tgtatttgcc caaaaatatg tagagttaaa tcgtccggat agagatgaac
     9241 taccctatta ttttggtatg accttagcag ttaaccaaag ctatttagct gctactaaac
     9301 atacaaaatt tattaacgat gacagtatat tatctgttca accaggggca agtagtggaa
     9361 tttctttagg tttattgggt actttaaaat taaactactg ttgggagttt aggtttaacc
     9421 cacagcttat cattggtggt tcaaaacatt tcatctatca aaaaaattat cctgtagaag
     9481 aggttaaaaa aacactccct tctaccattg ttagttttcc atttcagttt aaattaaatt
     9541 cagacaggtt aaataatttt agaacctatg tactatttgg tgctaaatac gatattgatt
     9601 tagcaagtaa ttctagtgcc agaaatgcac aagacatggt aaaattgaaa aaaagcgatt
     9661 ttgcactcga agcaggtatt ggtttcaact tttttttacc atttgtaacc attagtcccg
     9721 agcttaaatt tagttatggt ctaagtaata ttcatagcag agaccctgca ttaaaatatt
     9781 caaatgtttt cgataaaatt caatctagaa tgatttccct ttctatacat ttagaagatt
     9841 agttgtatct tcctacatta aaatacaact ataatattaa ttagcttaag ctattaaatt
     9901 tatagttatt tcataaaaaa attctactaa aaacaataac acaaaaaata aaattatatg
     9961 aaagaactaa ttgaaaaatt aagtacagaa gtaggcttaa caccagagca agccaataaa
    10021 gcaatagaag ccattgcaag ttttgtaaaa gaaaaatttc cgatgttggg cggtgctgtt
    10081 gatagtattt ttagcaaaga agaagagtaa aatgtttatt aaatagaagt ttaattaaat
    10141 aacatcttct tcaacttgtt actattatta tagctgaaaa atttaaagac accaaagtgt
    10201 ttttaagcca taatgtacaa aaaataactt gccaatttag gcaacattag ttcaattatt
    10261 gttctaattc atttacatta attctactta aaaacatata tttcttctat aaatctttct
    10321 gcacaatatg tgtgcataaa ctagtttttt attgtaacaa cttttgtaaa ttgtgcataa
    10381 atagcaagtt tactgttagt ataattaaat ttttattgaa ttaaattgtg aatagctgtg
    10441 aattaataaa tgctgcctaa aaagatttta atactccatt tctgtagcat attcgctaac
    10501 actatttatt tttttaaaaa aattataaaa aaaacagatt acaaaacaca ataacaaacc
    10561 ccttaaattt ttgttttatt aacccatgga aataattagc aacttaggaa gagataataa
    10621 gaaaagacga aaatcaaata ttgatttaag tacaatggta tatggcaaag taccaccaca
    10681 agcaagagag ttagaagaag cagtattagg tgcaattatg ctagaaaaag gtgcatttga
    10741 taaagtggtg gatgtaaatc ttaaatcaga atgtttttat gtggaagctc accagcatat
    10801 ttataaggca atgcaagata tgcagcaaca aagcattcca atagatattt taacagtagt
    10861 agaagaactt aaaagaaaag aattattaca acaa
//
LOCUS       DAOQPK010000055        10891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_3123_length_10891_cov_7.709948, whole genome shotgun sequence.
ACCESSION   DAOQPK010000055 DAOQPK010000000
VERSION     DAOQPK010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10891
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3123_length_10891_cov_7.709948"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(302..6496)
                     /locus_tag="PLU36_04165"
     CDS             complement(302..6496)
                     /locus_tag="PLU36_04165"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HMZ45975.1"
                     /translation="MNKFYTVFKYYFLLLLLFAFSADILGQATYKLIKSTSKLVADTK
                     NIFSKAVVMAFFILAFSANSFGVDVTHTFSSTNGSIDANINFTTQQNSSANAPAFSGN
                     LRLYYNSGGDGCSITLTPSNGAVITAVELYAVSSYTPTVKYNVDGGTDATASLSSTTY
                     TISSISASSSLKIRNANTSNTQLRLTGIKVTYTPKYTVTYNGNGSNGGTVPSDASSPY
                     ATGSTVTVLGPGTMTKTGNTFAGWDSDNNGSADFTGSGSDTFTITANTTLKAVWTPSS
                     SPSLSATALTNFGTQCEGGTYGPNMFTITGANLTTVDVTVAALSGFTYSTTSGGTYNT
                     SLSLSQSSGAYSQDIYVKFSPTSGSSYNGNIVVGGGGASNINIAASGIGLAKATPSVS
                     IASTPAAVSGTTTVCSGTNVSFTATASALGGGTASYQWKLNGTNVGTNSNSYSNNTLT
                     TGNTISCDITVSGGCVAATTASSNTITMAVNPSPSNDLCSNAVSLTANAAAVSGTMTP
                     CAGFESPFSGSKKDVWYKFVAPCNADYTITLTGFSGDMDMSLFYNSNSCPSSTTSLSS
                     STTSTPTETITYTLTSGNTYYIRVYAFNTAAESSPFTIGVTGITTVPAQPDPIAGETS
                     VCPGIEEVYSTNTVANATGYTWAVPTGWTITAGAGTTSITVTTGSSGGTVSVTADNGC
                     GSSTVRNVAVSTLTVPTAPTATAGTAITGASFTANWNSVGGITDYSVDVYTYNTNQNI
                     AAWTFPTSGTITTADIYNANNNGKTITRQGGGSITDIIGATTRAAYGDGGWNSGSGSK
                     FWQILIDASDSKNLTLSSKQQSSNSGPRDWKVQYKIGSGGTWTDITGGNITVANNFTT
                     GVLTNLALPSACDFQSELYIRWIMTSNTQSDGGGSVASGGTSRIDDIYVNGDMFSYVP
                     GYENIITSGTSLAVTGLSPNTDYYYVVRADNGNCVSPNSNTIKVTTSSCASATHTVTS
                     ILPNTGPVGTRVLIRGTGFTSITDVQIGGISITDYTVVDNTTIIARIPDGATTSTIDV
                     YNNGCPKPSGNFTVLSSVGLCGGGASVTPTDLLISEVYDSETGSRGYIELYNGTASTI
                     NLSNYKIKIYTGATTWVSLSGTLAPNKFAIVKIGSTGTDCDAVIAATNALVIDRSASS
                     GYNENDPIELYKGNTKIDRMVTPNEKGFNMERNTLTDAPSTTFTSANWTKTSTETCSS
                     LGNPSFNVAPLLVINTNATDWTTCPGTVQFTVSAIATPAPVTSYTWYYNDFNLMTGWQ
                     LVSGNITGATGTNTATLILPADFAPLYNYQFYCDINKTNSGNSCTKATNAAYFTYPTA
                     RYYRTKTTTSNGSWTTPSEWEMSTDINFVNPAPITACAYPLSVNSSAAFVQNGTKTTI
                     GDGISSYVIDIDKITIDGTLELHKKSSLFVYDSTASGADMIVNGTLIDRAGSSDGIYF
                     TGTSANNGSGWTLGNTATVIKTNGSSAVVYKDWYQDGISTIPDGSAGAKWIYRYIGDG
                     NPSTNAVGMFYPNLAFENTGSATNFPFNNSLMALTGASGGYCTVKGNMDIGTSGIGTV
                     TVYNNNINSQAMKVLGNMTIGNGSTFTNTSYDGVTNATRGHGTGIELYGNLTNDGTFT
                     NNSNSIGILRFKGTGTQTVSGAGTFNLYNVELDKPSQTLVDQQVNLTAQNNLNFNAGG
                     ILKTNANVFSVNNGSIANAVTGHETAYTGAATPNYSNDKYVFGKLERVVNTNGIYDFP
                     VGDDVAGEGYNPIRFERISGTGNATAWFIPGDPGIINTGGPIGVSCTGRNFYHYTDMT
                     GEGKWRMESGTSTNFNYNVYLHPNQNNANNHPNDDGTNYPYFYKNNYRALKAPNGTND
                     WTPYAGLVDPCVVGSYYNTPGADYTGFSDFAIPGGDGLSTALPVELTSFTATCNDNNA
                     VDIRWTTASELNSSAFMVQRSVDGIHFSTIATLAAAGNSSIPRHYVVIDSNAVTSNNY
                     YRLIEVALDGQQTIYSFLQVRCAEVNGMNVYYNQPKVVVEVSATKDKEVNFQVFEISG
                     KLLHQETKILTRGYNKFDLSIQNRLANGIYIIQMHDEDRQSSMKIMIH"
     gene            complement(6852..7382)
                     /locus_tag="PLU36_04170"
     CDS             complement(6852..7382)
                     /locus_tag="PLU36_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45976.1"
                     /translation="MSACKKEKDDPNITQFTYNRTITNPGAYPSSNVDSLDVNQDGAF
                     DLYFIIERETADSQYVAYEADHLALALNDAPVFGGFKFAKLFSKGNMPPLYNAITENY
                     YTDNYISLILDGVSYGADKKGNVYLAFGMKPSSSSSDIYYGWIQIAIANDYSTIKCID
                     GAISDIPNKTIAIGSK"
     gene            7888..8643
                     /locus_tag="PLU36_04175"
     CDS             7888..8643
                     /locus_tag="PLU36_04175"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HMZ45977.1"
                     /translation="MKLLISAISLLLTLHVQAQFTSTNGSNEKYIEVKTTDTLMVNPD
                     AISLLIALGEKKEKNYYFDDDQTDAEKEEEARKEKNDAIQKKKQIEEILKKNNVSAFK
                     FHDKKEDKDLFTKEFNLYENTYEVELKTDAQVEKIKKEMIVLKDVSTKVANSKLNDKE
                     KYELILIDKVMKKAEREASAIAKTMNVTLDKALNVSNQSVNDIYSSMFNSPESMGGMG
                     ALFSMMGNMFKGATQQNTQVVVSKTLIVRFAIK"
     gene            8648..9049
                     /locus_tag="PLU36_04180"
     CDS             8648..9049
                     /locus_tag="PLU36_04180"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HMZ45978.1"
                     /translation="MFNKFSTDYGLLIIRIGIGVMFALHGFPKITGGPEKWEMIGKAM
                     ENIGIGFAPTFWGFMAAVAEFVGGICLVLGFLFRPATAMLLFTMFIAAFMHYVNGDGF
                     SGYSHAVESAIVFLGLFFSGPGKYALKISRR"
     gene            complement(9059..10138)
                     /locus_tag="PLU36_04185"
     CDS             complement(9059..10138)
                     /locus_tag="PLU36_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45979.1"
                     /translation="MSDSFSENNKNISNELQCKDCGALLLFAPGTEKLACKYCGAQNE
                     ILADVENQEIEEMDYHDFIANKIKQEDKQTVNTVNCNNCGATTTLKPNITSDNCAFCA
                     SPLVIKGGTTSTILKPKYILPFKVDDKTAHDIFIKWLHGLWFAPNDLKKYASVSEKLK
                     GVYMPYWTYDSNTNTSYDGERGEYYYEGSGNNKQRKTKWYPVQGTTQHAFDDVCVSAS
                     QSLPDNTMRTLEPWDLEALVAYKEAYISGFQTECYQTDVIKGYKTAKYLMEEAIKDKI
                     RREIGGDEQRIHNMHVAYSDIRFKHILLPIWISAYSYHSKVFQFIINGRTGEVQGARP
                     YSAIKIALAIIAVISLFVLMKYMGN"
     gene            complement(10220..>10891)
                     /locus_tag="PLU36_04190"
     CDS             complement(10220..>10891)
                     /locus_tag="PLU36_04190"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HMZ45980.1"
                     /translation="DAGKFMKEIVGTDGQFTTEEIANQLRALVVTKLTDAIAESKLKI
                     EEFASNLEEFSKFGAEKLSDDFDAYGLKVTSLLVENISMPDEVKKEIFELSRLDKIDM
                     QKLTQWKTAKGIEKAAENGGGLAGAFVGVGLGGIMQGGIAGSQQGGGAVPPPVMQIFV
                     AVNGAQTGPFDVPALTQMAQSGQLKADTLVWKAGMAAWAAASTLPELAAVLNSVPPPL
                     APPPL"
BASE COUNT         3304 a         2080 c         2026 g         3481 t
ORIGIN      
        1 atattaaaag cttaaaaata attgattctt atttactcat atagtaaatc aattactttc
       61 cgagcaacgt ctctcaaaga ggattatgtg aaatattgtg tactactttg atttacgcag
      121 agtccctttc aggagactct gtagagaagc aattcctttt aggagactct gctgagaaac
      181 aatatcgcca ccacaacgaa caaagacatt atccttaata gtcgaacggg tcacaagacc
      241 cgtcgaggca aataaaaaaa tgcactcaaa agagagcatt tataaaatat cgttgatttt
      301 attaatgtat cattatcttc atggacgact ggcgatcttc atcgtgcatt tgtataatat
      361 aaatgccatt ggccaaccta ttctggatac ttaaatcaaa tttattgtag ccacgagtca
      421 gtattttagt ttcttgatgc aataattttc cagatatttc aaatacctga aaatttactt
      481 ctttgtcttt cgttgcagat acttctacta ctacttttgg ttgattgtaa tacacattca
      541 taccatttac ttctgcacaa cgcacttgta aaaaggagta aatcgtttgt tgaccatcta
      601 atgccacttc tattaaacga taatagttat tgcttgtaac agcattcgaa tctatgacaa
      661 cataatgtct cggtatgctt gaatttcctg ctgcggcaag tgttgctatc gtactaaaat
      721 gtatgccatc tacagagcgt tgtaccataa atgcgctgct atttaattca gaagctgtag
      781 tccatcgtat atccacagca ttattatcat tacaagtggc cgtaaaagaa gttaactcca
      841 ccggaagtgc cgtagataat ccatcgccac ctggaatagc aaaatcagaa aaccctgtgt
      901 aatctgcacc tggcgtatta taataagaac ctaccacaca aggatctacc agaccagcat
      961 aaggtgtcca atcatttgta ccatttggag cttttaaagc acgataatta tttttgtaaa
     1021 aataaggata gttggttcca tcatcatttg gatgattatt tgcattattt tggtttggat
     1081 gcaagtacac gttataatta aagttagtag atgtaccgct ttccatgcgc cattttccct
     1141 cgcccgtcat atctgtataa tggtagaaat tgcgacccgt acaactaaca cctataggtc
     1201 cgcctgtatt aataataccc ggatcacctg gaataaacca tgctgtggca tttcctgttc
     1261 cgctgattct ttcaaaacga atgggattgt agccttcgcc agcaacgtcg tcgcccactg
     1321 gaaaatcgta aatgccgttg gtattgacaa cacgttctaa cttaccaaat acatatttgt
     1381 cgttgctgta gtttggagtt gctgcacccg tatatgctgt ttcatggcct gtaactgcat
     1441 ttgcaatact tccattgttt accgaaaaga cattggcatt tgtttttaaa ataccacctg
     1501 cattaaaatt caaattattt tgagcagtta agtttacttg ctgatctacc aatgtttgcg
     1561 atggtttatc aagttcaaca ttgtataagt tgaatgtacc agcacccgaa actgtttgtg
     1621 taccagttcc tttaaatctc aaaattccta tactattaga gttgtttgta aaagttccat
     1681 catttgttaa attgccatat aattcgatac cggtaccgtg tccacgagta gcattagtaa
     1741 caccatcgta agaagtatta gtaaatgtag agccatttcc aatcgtcata tttcccaata
     1801 ctttcatggc ttgtgagtta atgttattat tatatacagt aacggtgcct ataccgcttg
     1861 taccaatatc catattgcct tttacggtgc aataaccacc agatgcacca gtaagtgcca
     1921 ttagtgaatt attaaatgga aaattggtgg cggagcctgt attctcaaat gctaaatttg
     1981 ggtaaaacat ccctaccgca ttggtacttg gattgccatc gccaatataa cgataaatcc
     2041 attttgcacc agcgctacca tctggtatgg tactaatgcc atcttgatac caatccttat
     2101 aaacaacagc agaggagccg ttcgttttta ttacggtagc ggtatttccc aaagtccaac
     2161 cagagccgtt attggcagaa gtaccagtaa aataaatacc gtcagaagag cctgctctgt
     2221 ctattaaagt gccgtttact atcatatctg caccgcttgc agtgctatca taaacaaata
     2281 aggaagattt tttatgaagt tctaaagtgc catcaatcgt tattttatca atatcaatta
     2341 cataacttga tataccatcg ccaatagtag ttttggtgcc attttgtaca aatgctgccg
     2401 atgagttaac ggaaagcgga taagcacatg ctgttatagg tgcagggttt acaaagttaa
     2461 tatcggttga catttcccat tcacttggcg ttgtccaact accattggaa gtagttgttt
     2521 ttgtcctgta ataccgtgcc gttggatagg taaaatatgc agcattggtg gcttttgtac
     2581 aagagttacc actgtttgtt ttatttatat cacaataaaa ttgatagttg tataacggtg
     2641 caaaatcggc gggtaagata agcgttgccg tgttggtgcc tgttgcaccc gtaatatttc
     2701 cacttactaa ttgccatccc gtcattaaat taaaatcgtt gtaataccat gtataagaag
     2761 taactggtgc tggtgttgcg attgccgata cggtaaactg tacagtacct gggcaagtag
     2821 tccaatcggt ggcgttggta ttaataacca ataatggggc tacattaaag gatgggtttc
     2881 ctaatgagct acaggtttct gtgctggttt tagtccaatt tgccgatgta aaggtagtag
     2941 aaggtgcgtc agttaaggta tttcgctcca tgttaaaacc tttttcattt ggtgtaacca
     3001 ttctgtctat tttcgtattg cctttataca attctatggg gtcattttca ttataaccac
     3061 tagaagcact tcggtctatc actaatgcat tggtggctgc aataacagcg tcgcaatcgg
     3121 tgccggtgct accaattttt acaatagcaa atttattagg ggctaatgtg ccgcttaaac
     3181 ttacccaagt agttgctcct gtatatattt ttattttata atttgataaa ttaatggtag
     3241 atgctgttcc gttatacaat tctatataac cgcggctacc agtttcagaa tcatacactt
     3301 cggaaatcaa taaatcggta ggggtaacgc ttgcaccacc gccacacagc cctacgctgc
     3361 ttaatacggt aaagtttcct gatggttttg gacagccgtt attgtaaaca tcaatagttg
     3421 acgttgtagc accatcgggt attctagcaa taatagtggt attatccaca acagtataat
     3481 ctgtaatgga tatgccacca atttgtacat ctgtaatact tgtaaagccc gtaccgcgta
     3541 ttaacactct ggtgcctacg ggcccagtat ttggcaatat agatgtaaca gtatgtgtag
     3601 ccgaagcaca cgaagaggta gttactttta tggtattaga gtttggagaa acacaatttc
     3661 cattatctgc tcttaccaca taataataat cggtattggg tgataaccct gtaactgcca
     3721 aggaagtacc cgaagtaata atattttcat atcccggtac gtaagaaaac atatccccat
     3781 ttacatatat gtcatctatt ctacttgttc cgcccgatgc tacagaacct ccgccatctg
     3841 attgagtatt tgaagtcatt atccaacgga tatataattc tgattgaaaa tcgcaagctg
     3901 atggtaatgc taaattggtt aatacgcctg ttgtaaaatt attagcaaca gttatatttc
     3961 cacctgtaat atcagtccat gtaccaccac ttcctatttt atattgtact ttccaatctc
     4021 ttggaccaga attagaagat tgttgcttag aagataaggt gagattttta ctgtccgaag
     4081 catcaattag tatttgccaa aatttagagc cactaccact attccatcct ccatctccat
     4141 acgcagctct tgtagtagca ccaataatat ccgttatcga tccgccacct tgtcgtgtaa
     4201 ttgttttacc attattatta gcattataaa tatcggcggt agtaatagta ccacttgttg
     4261 gaaatgtcca tgctgctata ttttgattgg tattataggt ataaacatct acactgtaat
     4321 cggtaatgcc tccaacgctg ttccagtttg ctgtaaaaga ggcgccggta atggcggtac
     4381 ctgccgtagc ggtgggtgcg gtgggtacag ttaatgtact tacggctacg tttcttacgg
     4441 tactgctacc acaaccgtta tcggcggtaa cagaaactgt accgccactg ctaccagtgg
     4501 ttacagtaat agaagtagta ccagcacctg cggttattgt ccaacctgtt ggtactgccc
     4561 acgtataacc agttgcattg gcaaccgtat tggtcgaata aacctcctca atacccggac
     4621 aaacggaagt ttcaccagca attgggtcgg gttgtgcagg tacggttgtt attcccgtta
     4681 caccaattgt aaaaggagaa gattcagctg ctgtattgaa tgcataaaca cgaatataat
     4741 aggtattgcc acttgttagt gtatacgtta ttgtttctgt tggagtagaa gtagttgaac
     4801 ttgataaaga agtagttgaa cttggacatg agttgctatt ataaaataat gacatatcca
     4861 tatctccact aaatcctgta agggtaatcg tataatcggc attgcagggt gcaacaaatt
     4921 tataccaaac atctttttta ctaccagaaa aaggactttc aaatcctgca caaggtgtca
     4981 ttgtaccgct tactgcagca gcatttgcgg ttaagctcac ggcattgcta cataaatcat
     5041 tacttggtga agggtttacc gccatagtaa tggtattgct actggctgtt gtggctgcaa
     5101 cacaaccacc acttaccgta atatcgcaag agatggtatt gccagttgtc aatgtattgt
     5161 tgctatatga attgctattg gtacctacat tagtgccatt taatttccat tggtaacttg
     5221 ccgtacctcc acctaaagca gaagcagtag ccgtaaatga aacattagta ccactacata
     5281 ccgttgtagt acccgaaact gccgcaggtg tagaggcaat gctaacgctt ggtgtggctt
     5341 ttgccaaacc tatgccactt gccgctatgt tgatattact ggcaccgccg ccaccaacaa
     5401 caatattccc attgtaagaa ctgcctgaag taggtgaaaa ttttacatat atatcttggc
     5461 tatatgcgcc actagattga gataaagaaa gacttgtgtt gtatgtacct cccgaagttg
     5521 tagaatacgt aaaaccagat aaggctgcta cggtaacatc tacagttgtt aaattagcac
     5581 cagttatggt aaacatatta gggccataag tgccaccctc acactgtgtg ccaaaattgg
     5641 taagtgcagt agcagataaa cttggtgagc tgcttggtgt ccataccgct tttaaggttg
     5701 tattagcagt aatggtaaat gtatcgctac cagaacctgt aaaatctgca gaaccgttat
     5761 tgtcgctatc ccagccggca aaagtattgc ctgtttttgt catagtgcct ggtcctaata
     5821 ctgttacagt agaacctgta gcataaggac tgcttgcatc acttggtaca gttcctccat
     5881 tactaccatt gccattgtag gtaacggtgt attttggtgt ataggttact ttaatgcctg
     5941 ttagccttag ttgggtatta gacgtattag cattccgaat ctttaaagaa gatgaagcag
     6001 atatacttga tattgtatat gttgtactac taaggcttgc agttgcgtct gtaccaccat
     6061 cgacattgta ttttacagtt ggtgtataac tagaaactgc atataattca actgctgtaa
     6121 ttactgcacc attagaaggt gttaatgtaa ttgaacaccc atcaccacct gaattataat
     6181 ataatctcaa attaccgcta aaagcgggag cattagcaga agagttttgt tgagttgtaa
     6241 aattaatatt agcatcgata gagccgtttg ttgaagaaaa agtgtgtgta acatctacac
     6301 caaaactatt agcagaaaaa gccaaaataa aaaaagccat aacaacagct ttgctaaaga
     6361 tattttttgt gtcggcaact aatttcgaag tagatttaat tagtttatat gttgcttgcc
     6421 ccaaaatatc tgctgaaaaa gcaaataaca acaacagcag gaaataatat ttaaaaacag
     6481 tgtaaaattt attcataaag tatcttttta aaaaaacgaa cggctcggtt tacagaaacc
     6541 ggacatcaaa tatacatcaa gatactctaa tcgtgctata tagtcaagtt aaatttttgc
     6601 gcttttttaa tgtattttat tggaaatgta cacattgtaa aaaatcatta tacgttttgt
     6661 cgttcatgct tgtataaaaa cgaacgcaag ccatcatggc gaagtacgaa gcaatctcat
     6721 acagcatcaa cctaataaga attgaaacaa atgagatggc cacgcttcac agtctatggt
     6781 ataagtttta cctgctttct tgtttcgctc gccatgacac aactgagaaa taaaaaataa
     6841 aaatttagtt tttatttact gccaatagct attgtcttat tcggaatatc gctaatggcg
     6901 ccatctatac attttatggt gctgtaatca ttggcaatag caatttgtat ccagccgtag
     6961 taaatatctg agctagacga acttggtttc ataccaaatg ccaaataaac atttcctttt
     7021 ttgtcggcac cataagaaac accatctaat attaaagaaa tataattatc cgtataatag
     7081 ttttctgtta tcgcattata caagggaggc atattgcctt ttgaaaataa tttcgcaaac
     7141 ttaaatccgc caaaaacagg tgcatcgtta agtgccaatg ccaaatgatc ggcttcatac
     7201 gccacatatt gtgagtcggc tgtttctctt tctataataa agtataagtc gaatgctcca
     7261 tcttgattga catctaagga gtcaacatta gaacttggat aggctccagg attggtaatg
     7321 gttctgttat aagtaaattg ggtaatatta ggatcgtctt tttctttttt acaagctgac
     7381 ataaccataa cgaataaaga gataaagcaa aagatgctgt ttttcgtaag tgttttcatt
     7441 gtattgaatt aatagtagtg attaatatct caaagatgtc atatctttaa ataagtatca
     7501 atgcttattt agtatttgat actatttaat gagaattcgt gaagaaaatg gcagaagatt
     7561 tttccgcttg caatgatgaa catgagccag catgtgtaaa aaaatacgca ttgtgctgtt
     7621 taaattaaag ttggattttt ttattcaatt gattatcaaa ttcattcttt tgtcactttt
     7681 tttcttagtc aaaaaaagta accaaaaaag acaaggcaaa accaaatgct ccgctggttt
     7741 tgccaaccca cgcttcttta catacactaa attttacctt tcgttgtttt tttattgaaa
     7801 attataattt tcaaaccgta caacgggtta aaaaaatgta tttacttagc agaaaatata
     7861 tatatttact catcaaaaac actacttatg aaattattga tttctgcaat tagcttactg
     7921 cttacacttc acgtacaagc acaatttaca agtacaaacg gtagcaatga aaaatatatt
     7981 gaagttaaaa ccacagatac tttaatggtt aatcctgatg ccatctcctt gttgattgca
     8041 ttaggtgaga aaaaagaaaa aaactattac ttcgacgacg accaaactga tgcagaaaaa
     8101 gaagaagaag cgcgaaagga aaaaaatgat gccattcaaa agaaaaaaca aattgaagaa
     8161 atattgaaaa aaaataatgt ttccgcattt aagtttcacg ataaaaaaga ggacaaagac
     8221 ctatttacca aagagtttaa tctgtatgaa aatacatacg aagtggaatt aaagacagat
     8281 gcacaagtag aaaaaataaa aaaggagatg atcgtattga aagatgtgag caccaaagtg
     8341 gctaattcaa agctgaatga taaagaaaaa tacgaactca tccttatcga taaagtgatg
     8401 aagaaggcag agcgcgaagc aagtgccatc gcaaaaacta tgaatgtaac attggacaaa
     8461 gcattaaatg tttcgaatca aagtgtaaat gatatttatt cttcaatgtt taacagtccg
     8521 gaaagtatgg gcggcatggg tgccttattt tcaatgatgg gaaatatgtt taaaggcgcc
     8581 acacaacaaa atacgcaagt agttgtttct aagacattga ttgttcgatt tgcaataaaa
     8641 taaaattatg ttcaataaat tcagcacaga ttacggctta cttattatca gaataggaat
     8701 tggtgtcatg tttgcattac atggatttcc gaaaataact ggtggtcccg aaaaatggga
     8761 aatgattggc aaggcgatgg aaaatatagg catcggtttt gcgccaacat tttggggttt
     8821 tatggctgca gtggcagagt ttgtcggtgg catctgttta gtgttaggtt ttctatttag
     8881 gcctgcaaca gcgatgctct tatttacaat gtttatcgca gcgtttatgc attatgtaaa
     8941 tggtgatgga ttttcgggct attcgcatgc tgttgaaagc gccatcgtat ttctaggatt
     9001 gtttttttct ggtcctggaa aatatgcatt aaaaatttcg agacgataat ctgttctctt
     9061 aatttcccat atatttcatc aacacaaaaa gcgatatcac tgcaataatt gctaacgcga
     9121 ttttaatggc gctgtatggt cttgcgcctt gaacttcgcc tgttcttccg tttattataa
     9181 actgaaatac ttttgagtga tagctgtatg cacttatcca aataggcagt aaaatatgct
     9241 tgaaacgaat atcactatag gcaacatgca tattgtgaat tcgctgttcg tcgccgccaa
     9301 tttctctgcg tattttatct tttatggctt cttccatcaa atacttagct gttttatagc
     9361 ctttgatgac atctgtttga taacactcag tctgaaaacc actaatgtat gcttctttgt
     9421 atgcaactaa ggcttctaaa tcccaaggct cgagtgttcg catggtatta tctggcaacg
     9481 actgacttgc cgacacacaa acatcatcga aagcatgttg cgtagtgcct tgtactggat
     9541 accatttggt tttacgttgt ttgttgttgc cggagccttc gtaataatat tcaccacgtt
     9601 cgccatcata actcgtgttg gtattagaat cgtaagtcca ataaggcata tatacacctt
     9661 ttagtttttc agaaactgat gcatattttt ttaagtcgtt tggtgcgaac cataatccat
     9721 gtaaccattt tataaaaata tcgtgtgctg ttttatcatc gactttaaaa ggcagtatat
     9781 attttggttt taatattgtt gaagtggttc cgcctttaat cactaatggc gatgcacaaa
     9841 aggcgcaatt atctgaagta atattcggtt ttaaagtagt tgttgcgccg cagttattac
     9901 aatttaccgt gtttacagtt tgtttatctt cttgcttgat tttattggct ataaaatcat
     9961 gatagtccat ttcttcaatt tcctgatttt ctacgtctgc aagaatttca ttttgtgcgc
    10021 cacaatattt acacgctaat ttttctgtgc caggtgcaaa aagtagcaat gcgccacaat
    10081 ctttacattg tagttcattg cttatgtttt tattattttc agagaaagag tcgctcattt
    10141 tttggtttta tggtcgaaag aaaaaagact tgacacgttt ttgcttgtgt caagtcctac
    10201 atattgttga atatactcat tacaatggtg gtggtgccaa tggtggtggc accgagttaa
    10261 gcactgctgc cagctctggc aaagtagatg ccgcagccca tgctgccatg cccgctttcc
    10321 acactaaggt atccgctttt aattgtccgc tttgtgccat ttgtgtcaat gcaggcacgt
    10381 cgaatggtcc ggtttgtgcg ccgttgacgg ctacaaatat ttgcatcact ggtggaggca
    10441 ctgcgccgcc gccttgttgc gagccagcaa taccaccttg cataatgcca cctaagccta
    10501 cgccaacaaa agcaccagcc aagccgccgc cattttcggc tgctttctca ataccttttg
    10561 ccgttttcca ttgtgtcaat ttctgcatat caattttatc caaacgagag agttcgaaaa
    10621 tttctttttt tacttcgtct ggcattgata tattctccac taatagcgaa gtcaccttta
    10681 agccataagc atcaaagtca tcggataatt tttctgcgcc gaactttgaa aactcttcta
    10741 agtttgaggc aaactcctca attttgagtt tgctctctgc aatagcatct gtcagcttgg
    10801 taaccaccaa agcacgcaac tgatttgcaa tttcttcggt agtaaactga ccatcggtac
    10861 ctacaatttc tttcataaat tttccggcat c
//
LOCUS       DAOQPK010000056        10761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_3186_length_10761_cov_4.148982, whole genome shotgun sequence.
ACCESSION   DAOQPK010000056 DAOQPK010000000
VERSION     DAOQPK010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10761
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3186_length_10761_cov_4.148982"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..499
                     /gene="hpt"
                     /locus_tag="PLU36_04195"
     CDS             2..499
                     /gene="hpt"
                     /locus_tag="PLU36_04195"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HMZ45981.1"
                     /translation="MSEQEIADKIQQLATQLKQDYDGKKPLFLAILNGAFMFASDLFK
                     TLEIEAEICFIKLASYKGTKSTGHVITAIGLDTDLTNRHVIILEDIIDTGKTLHSFLP
                     QVYNQQPASLKIAVLLDKPEPRQFDVPVDYACFVIPNKFVLGYGLDYDGFGRNTKDIF
                     ELFEG"
     gene            569..1633
                     /locus_tag="PLU36_04200"
     CDS             569..1633
                     /locus_tag="PLU36_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HMZ45982.1"
                     /translation="MNLIRLTILILPLVFLLSCKKHTDTYTNTLYFPPNSSTQWDTMS
                     STEAGFNASKLQALKDYLAITNTKSFMIVKNGKIVVEEYYNSHTSSSTWQWNSAGKTL
                     VATITGIAQQDGFLNINNKVSDYLGNGWTSENLLKENLIKIKHLLTMTSGLNDENQLV
                     IKSNLTYLADAGTRWSYHNVFQKLIDVVASATSQNFENYFNNKLRDKIGMDGFWNFGT
                     IFKIYHSNTRSMARFGLLTLNKGKWNNEQIINENFLLESTTPSQSINPSYGYFWWLNG
                     KSSYMLPNAQTVYNSTLVPNAPIDMIAAMGAEDQRLYIIPSKNIVVVRMGDASDPSNP
                     TFALSGFDNTFWSKLSAFIN"
     gene            1803..2288
                     /locus_tag="PLU36_04205"
     CDS             1803..2288
                     /locus_tag="PLU36_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45983.1"
                     /translation="MKNKRWFLNALILILVVTTSCKPLKEIKPETMVENNLIKYEKKP
                     FTSTHVFTAVFKNIQFHTCKGLTALCPKDCGNSGYMANFSVLNYRTDIVNGQAGTEKL
                     DTYTIQLGDFYKKDFDKDYVRFIRKLKQGNEVTMHIDYIYDATKSAVMTVEKIVSISK
                     N"
     gene            2299..2733
                     /locus_tag="PLU36_04210"
     CDS             2299..2733
                     /locus_tag="PLU36_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMZ45984.1"
                     /translation="MARVSTYLNFPGNTEEAFNFYKSIFNTQFVNDIVRFDSIPYDPN
                     NPTILEEVKKMVLHIELPILGGHIIMGTDAPKEMGFGVNNGNNMHINLEPDSREQAKY
                     LFDALSEDGKVEMPIQDMFWGAYYGSLIDKFGINWMINFQIK"
     gene            2735..3556
                     /locus_tag="PLU36_04215"
     CDS             2735..3556
                     /locus_tag="PLU36_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HMZ45985.1"
                     /translation="MKIIRVWFTFFLLLFFSCKIYSQVSDTIPTHDSIIFFSKQLKEN
                     RKINIWLPKQYKNSKASFEVVYMLDGGIKEDFPHLANTISELINQHKIKPIILVGIEN
                     TQRRRDLTGFTVNEKDKEIAPVVGGSEAFRAFIKNELFEEINKRYRTKDTKTIIGESL
                     AGLFITETFLSYPAMFSNYIAFDPSLWWNNEYLVKEADNFLNKFTNQPKNFWFAASGV
                     QGIYKPTNELASIFKNKHLSNFNYKYSFEPKEDHSTIFKATKEKALIWMLGSTHK"
     gene            complement(3609..5336)
                     /locus_tag="PLU36_04220"
     CDS             complement(3609..5336)
                     /locus_tag="PLU36_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HMZ45986.1"
                     /translation="MKFLISCCLLLSLSISFAQKTITTKFELSKGTQTPTYFEIIDWW
                     KKLDNQSGKIKLLTMGNTDAGYPLHLAVISNNGDYNFNSIRKNNKRIIFINNGIHPGE
                     PDGIDASILLARDIVVDKQKLPDNIVLCIIPVYNIGGCLNRSKNFRVDQNGPEEFGFR
                     GNAQNLDLNRDMIKCDSKDARAFTEIFHLLDPDVFIDNHVSNGADYQHIMTLLTTQQS
                     KLGGVMGDYISKKFEPSIYSLMKQKGYDLIPYVNVWGNTPDKGWTEYWDSPRYASGYA
                     ALWHTFSFVPESHMLKTYEQRVKATYTLMQSFIEFTSANSKEIKVLREQSKQLAKSAV
                     DFPIKWSVDKSKFKEVNFFGYESGLKNSEVSGMPRLFYDKTKPYQKTVKIYNYFTPKE
                     FVKKPSAYIIPQGWWKVIELLQLNKIEMISLKKDTIIEVEVYKIEDYKTSSKQYEMHH
                     LNSEVKYTTSIKKVNFRKGDWYIPLNQVANRFLIETLELSGEDSYFSWNFFDAILGQK
                     EGYSDYVFEDIAANYLKNNSALQEKLNQKKLTDTTFAKSGRAQLNFVYQNSPWFETAF
                     MVYPVYRVL"
     gene            complement(5520..5822)
                     /locus_tag="PLU36_04225"
     CDS             complement(5520..5822)
                     /locus_tag="PLU36_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45987.1"
                     /translation="MANILEYILSLKDQTKKTLANIGINSDRAYNKFNALKQQANEVS
                     RVMKVFGGSVGALRQKLELLKNERDWLPAKEINTIRAYNSEIKKLTAQITKFETIK"
     gene            6182..6415
                     /locus_tag="PLU36_04230"
     CDS             6182..6415
                     /locus_tag="PLU36_04230"
                     /inference="COORDINATES: protein motif:HMM:NF016148.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HMZ45988.1"
                     /translation="MVILIQHIAAAQSAAQYHEYMQDAERYNLQIRTAGDDKVRASHA
                     ELNGITLPANHEYWDTTWTPFDWRCRCRIIQVL"
     gene            6479..7087
                     /locus_tag="PLU36_04235"
     CDS             6479..7087
                     /locus_tag="PLU36_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45989.1"
                     /translation="MFRYNPGKQGVIFPEKHPYYGISKNALEVVKAMQQVEQSKRIDL
                     NKYIKNNVTAKIVRDVMLEYSNNFPENFLNGLEKIKFTSSQSYMMQHTINVNNITRKR
                     VGKSTISISNQTLRNGYNPAKEFIDGLASIKQGRELTFNQEYAFESMWHEILHAKTAS
                     QHHKLSNRQKEHMETVNQFVARHTYPKFIEAFDGKASNQMKY"
     gene            complement(7231..7461)
                     /locus_tag="PLU36_04240"
     CDS             complement(7231..7461)
                     /locus_tag="PLU36_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45990.1"
                     /translation="MDTIFDYNPTEQELVGLFYKTENEYKQNTSNDMIIADLAILFAD
                     RNQPQKANEYWAKIPELHQEFLLGFDDVLIPQ"
     gene            7480..7644
                     /locus_tag="PLU36_04245"
     CDS             7480..7644
                     /locus_tag="PLU36_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45991.1"
                     /translation="MQQFETFFKPILKDLKVELTDEFDRNFERKAFFYFDICDKDLLQ
                     CADAVIRLRA"
     gene            complement(7852..8613)
                     /locus_tag="PLU36_04250"
     CDS             complement(7852..8613)
                     /locus_tag="PLU36_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HMZ45992.1"
                     /translation="MEHYNMNLNLKVWRQKNSKTRGEFKMYRVENISSEMSFLEMFDV
                     LNERLIAEGDEPIAFDHDCREGICGMCSMYINGKPHGPWQGNTTCQLHMRAFKDGDTI
                     VVEPWRSNAFPVIKDLVVDRTAFDRIIQAGGYISVNTGNAVDGNALPINKDKADASFA
                     AAMCIGCGACVAACKNSSAMLFLSAKVSHLALLPQGEPERNSRVLSMVAQMDKEGFGS
                     CTNTGACEATCPKEISISYIAKLNQEYLCASLTAE"
     gene            complement(8660..9106)
                     /locus_tag="PLU36_04255"
     CDS             complement(8660..9106)
                     /locus_tag="PLU36_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45993.1"
                     /translation="MVKIVVVFFVLLFTLGCNQQPNVNENKNVEKPTIIGLWEVKFIC
                     ETDFTMDSRDTLRGTFCHSINNQFIKFNKDSIFYIEDSLITDTYKYSKYNDSVLNVQN
                     IKTGIQMKMYIQNITLSNLKFKNIRIDSNRQEEYEIMIDCIKTKKE"
     gene            complement(9119..>10761)
                     /locus_tag="PLU36_04260"
     CDS             complement(9119..>10761)
                     /locus_tag="PLU36_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HMZ45994.1"
                     /translation="RLAEVSANIIDQCVAQGVPFAREYGGLLSNRSFGGTQVQRTFYA
                     AGQTGQQLLIGAYQALERQVALGNVKMYNRHEMLEVVTIDGKAVGIIARDLISGKLQR
                     HFGNAVLLCTGGYGNVFYLSTNAMGSNVTAAWKAHKAGALFANPCFTQIHPTCIPVSG
                     DHQSKLTLMSESLRNDGRIWVPKNQGDNRKPNEIPESERDYYLERRYPAFGNLVPRDV
                     ASRAAKERCDAGYGVGTSKQAVYLDYASAIERYGKIEITKHGREVEDASSAEIITLGK
                     QVVKEKYGNLFDMYEKITGENPYEVPMRIYPAVHYTMGGLWVDYELMTSVKGMYCLGE
                     ANFSDHGANRLGASALMQGLADGYFVIPYTIGNYLADEIYSKPISTDHEAFVNAEKEV
                     GDRIRTLMNIKGNTSVETFHKRLGKIMWDKCGMARNEKGLQEAIVEIQQLRKEFWNDV
                     RIPGDIDEFNPELDKAGRVADFLELGELMCKDALLRKESCGGHFREESQTEEGEALRN
                     DAEFMYVAAWEFEGESNWKLHKEALEYEVIKPTQRNYK"
BASE COUNT         3653 a         1790 c         1525 g         3793 t
ORIGIN      
        1 tatttcagag caagaaattg ctgataaaat tcaacaatta gctactcagc ttaaacaaga
       61 ttatgacggc aaaaaaccct tgtttcttgc tattttaaat ggtgcattta tgtttgcatc
      121 agaccttttt aaaaccttag aaattgaagc agaaatttgt tttattaaac tagcttccta
      181 taaaggcaca aaaagcacag gccatgtaat tactgctatt ggcttagata cagatttaac
      241 taatagacat gttattattt tagaagatat tattgatact ggtaaaaccc ttcactcctt
      301 tttaccacaa gtgtataacc agcaacctgc ttccttaaaa attgctgttt tattagataa
      361 accagaacca cgtcagtttg atgtacctgt ggattatgca tgttttgtta ttcctaataa
      421 atttgtttta gggtatggtt tagattacga tggatttgga agaaatacaa aagatatatt
      481 tgaacttttt gaaggctagt ttattttttt aaatttcaca ctttctttta acctttttgt
      541 aatttgtagt ctaattgaaa agttaattat gaatttgatt aggttaacca tacttatact
      601 tccacttgta tttttattaa gttgcaaaaa acatactgat acctatacaa atactttata
      661 ctttccgcct aatagtagca cacaatggga tacaatgtct agtacagaag ctggttttaa
      721 tgcaagtaaa ctacaagcat taaaagatta tttagctatt accaatacaa aatcatttat
      781 gattgttaag aatggtaaaa ttgttgtaga agaatattat aatagtcata caagctcatc
      841 cacatggcaa tggaatagtg caggtaaaac tttagttgca acaataacag gtattgcaca
      901 gcaagatgga tttttgaata ttaataataa agtatccgat tatttaggta atggatggac
      961 ttctgaaaat ttactaaaag aaaatcttat aaaaattaaa cacttgctta caatgacaag
     1021 tggcttaaac gatgaaaacc aattggtaat aaaatcaaat ttaacttacc tggcagatgc
     1081 tggtacaagg tggtcttacc ataatgtatt tcaaaaatta attgatgttg tagcctctgc
     1141 aacatctcaa aattttgaaa attattttaa taataaatta agagataaaa ttggtatgga
     1201 tggcttttgg aattttggca caatttttaa aatttaccat agcaatacac gaagcatggc
     1261 aagatttggt ttacttacac ttaataaagg caaatggaat aatgagcaaa taataaatga
     1321 aaatttttta ttagaaagta caaccccatc tcaatcaatt aatccatctt atggttactt
     1381 ctggtggtta aatggaaaat caagttatat gttaccaaat gctcaaactg tatataattc
     1441 aacacttgtg cctaatgcac caattgatat gattgcagct atgggtgcag aagatcaacg
     1501 actatatatt attcctagta aaaatattgt agttgttaga atgggtgatg catctgaccc
     1561 ctctaatcca acttttgcat tgtctggctt tgataacact ttttggagca aactatctgc
     1621 atttataaac taattttatt taaccaaacc atgttacaat ttctaaccta ttcctttatt
     1681 tagaaaaaca aatagtacca taatattaat tgttaaagta aattattttt tattacatat
     1741 taatacttta ggcatcatta atactaaaat gaaaatcata tattatgcag tgtattaaaa
     1801 taatgaaaaa taagagatgg tttttaaatg ctttaatttt aatattggtt gttacaacct
     1861 catgcaagcc tttaaaagaa ataaaaccag aaacaatggt tgaaaacaac ttaattaaat
     1921 acgagaaaaa accatttaca tccactcatg tatttacagc agtatttaaa aatattcaat
     1981 ttcatacatg taaaggatta actgcacttt gtccaaaaga ttgtggcaac tctggctata
     2041 tggcaaattt tagtgtttta aattaccgaa cagatattgt aaacggacag gcaggaactg
     2101 aaaaacttga cacttatact attcaattag gagattttta taaaaaagat tttgataagg
     2161 attatgttcg ttttattaga aaattaaagc aaggcaatga agtaaccatg catattgatt
     2221 atatttatga tgccacaaaa tcagcagtta tgaccgtaga gaagattgta agcatttcta
     2281 aaaattaaat ttaattttat ggcaagagta tctacttatt taaactttcc aggaaataca
     2341 gaagaagctt ttaacttcta taaatctatt tttaatactc aatttgtaaa tgatattgtt
     2401 agatttgatt ctatacctta tgaccctaac aatcctacca ttttagagga ggttaaaaaa
     2461 atggttttac atatagaatt accaatatta ggtgggcata taattatggg tactgatgct
     2521 ccaaaagaaa tgggttttgg ggtaaataat ggcaataata tgcatattaa tttagaacca
     2581 gactctagag agcaagcaaa gtatttgttt gatgcactat ctgaagacgg caaagtggag
     2641 atgccaattc aagatatgtt ttggggtgct tattatggta gtttaattga taaatttggg
     2701 ataaattgga tgataaattt tcaaataaaa taagatgaag attataagag tttggtttac
     2761 attttttcta ctactttttt tcagttgtaa aatatattct caagtaagcg atacaatacc
     2821 tacacacgat agtataattt ttttttcaaa acaacttaaa gaaaatagaa aaataaatat
     2881 atggctgcct aaacaataca aaaacagtaa agcctctttt gaagttgttt atatgttaga
     2941 tggtggtatt aaagaagatt ttccacactt agccaataca atttcagagc ttataaacca
     3001 acataaaata aaacctataa ttttagtagg cattgaaaac actcagagaa gaagagattt
     3061 aactggtttt acagtgaatg aaaaagataa agaaattgca cctgttgttg gtggctcaga
     3121 agcttttaga gcttttatta aaaatgagtt atttgaagaa ataaacaaac gttaccgaac
     3181 aaaagatacg aaaacaatta ttggggaatc tttagcaggc ttgtttatta cagaaacatt
     3241 tttatcatat cctgcaatgt ttagcaatta tattgctttt gacccttcgt tatggtggaa
     3301 caatgaatac cttgtgaaag aagctgataa ttttttaaat aaatttacta atcaaccaaa
     3361 aaacttttgg tttgcagcat ctggtgttca gggtatttat aaaccaacta atgagttagc
     3421 atcaattttt aaaaataaac acttatctaa ttttaattac aagtattcat ttgaacccaa
     3481 agaagatcac agcactattt ttaaagcaac caaagaaaaa gcactgattt ggatgctagg
     3541 ctctactcat aaataaccac tttaatgaag ctgtttattg cattgctatt tgtatttttt
     3601 accaaaattt ataacactct ataaacgggg taaaccataa aagcagtttc aaaccaaggt
     3661 gagttttgat atacaaaatt taattgagct ctcccacttt ttgcaaaagt agtatctgtt
     3721 agtttttttt gatttagctt ttcttgcaat gcagaattat tctttaaata atttgctgca
     3781 atatcttcaa aaacataatc agagtaacct tctttttgac caagtatagc atcaaaaaaa
     3841 ttccaagaaa aataagagtc ttctccacta agttccaatg tttctattaa aaaacgatta
     3901 gctacctgat ttagtggaat ataccaatct ccttttctaa aatttacttt tttaatgctg
     3961 gttgtatatt ttacttcgct gtttaaatga tgcatttcat actgtttaga tgatgtttta
     4021 tagtcctcaa ttttataaac ttcaacctct ataattgtat ctttttttaa ggatatcatt
     4081 tcaattttat tcagttgtaa taattcaata accttccacc agccttgtgg aattatataa
     4141 gctgaaggtt tttttacaaa ttctttggga gtaaaataat tatatatttt tactgttttt
     4201 tggtacggtt tagttttatc ataaaataat cttggcatgc ccgaaacctc actatttttt
     4261 aaaccagatt cataaccaaa gaaatttact tctttaaatt tagatttatc tacactccat
     4321 ttaattggaa aatcaactgc agattttgct aattgttttg attgctctct aagcacttta
     4381 atttctttac tattagcaga tgtaaattca ataaaagatt gcattaaagt ataagttgct
     4441 ttaactcttt gttcataagt tttaagcatg tgagactccg gaacaaaaga aaaagtatgc
     4501 caaagtgctg cataaccact agcgtatctt gggctatccc aatattcagt ccaaccttta
     4561 tctggtgtat ttccccaaac gttcacatag ggtattaaat catatccttt ttgcttcatt
     4621 aaactataaa tagaaggttc aaattttttg cttatataat ctcccattac accaccaagt
     4681 tttgattgtt gtgttgtaag caaggtcatt atatgttgat agtcggcacc attacttaca
     4741 tgattatcta taaaaacatc ggggtctaat aaatgaaata tttccgtaaa agccctagca
     4801 tctttagagt cgcattttat catatctctg tttaaatcta aattttgagc attgcctcta
     4861 aatccaaatt cctctggccc attttgatcc actctaaagt ttttacttcg gtttaagcaa
     4921 ccaccaatat tgtaaacagg tataatacaa agtactatgt tatcaggaag tttttgttta
     4981 tctacaacta tatctctagc aagcaaaata cttgcatcaa taccatctgg ctcacctgga
     5041 tgaataccat tattaatgaa aattattctt ttattatttt tacgaattga attgaaatta
     5101 taatcaccat tattgctaat tactgcaaga tgtaatggat acccagcatc tgtgttaccc
     5161 attgtgagca attttatttt gccagattga ttatctaatt ttttccacca atctataatc
     5221 tcaaaataag ttggcgtttg agtgcctttt gataattcaa acttagttgt aattgtcttt
     5281 tgagcaaatg agatggataa acttagaagt aagcaacagc taattaaaaa tttcatacaa
     5341 tgtatttatt cattaaaaat aaaaaaggca tttcaaattg aaataccttt taaataattt
     5401 atttaataaa caacaagtaa tataaaatta ttcagcagtt aaactagcac ataaatactc
     5461 ttgatttagt tttgaaatat agctgataga aatttcttta gggcaagttg actctttact
     5521 catttaatag tttcaaattt ggttatttgg gctgtaagct tttttatttc actgttatag
     5581 gcacgtatgg tatttatttc ttttgcaggc agccaatctc tttcattttt aagtaattct
     5641 aacttttgcc ttaatgctcc aacgctgcca ccaaatacct tcatcactcg gcttacttca
     5701 ttggcttgtt gtttcaaagc attaaattta ttatatgctc tgtcgctatt aatgccaata
     5761 tttgccaacg ttttttttgt ttggtcttta aggcttaata tgtattcaag aatgtttgcc
     5821 aaaatttgta cttttacact atgggaatat tatttggaat aatttcaaca attggtatag
     5881 gtgtaattct gttttattgc tttttactac ttttgtgtat ggcaggtttt gtaattgcca
     5941 tgccagttat tattatacag ctatgttcta aaaaggaaaa cagacgaaaa atatggttgc
     6001 cactaatagc aattactata ttttcatttg cctgctggtt atggtttacc gaccattagc
     6061 attagattat tttcctgctt ctttctttct aatccactcc aattctttta ttcgcattgc
     6121 ccattgttca ttgctaaggc tgtctagatt ttatatgtgc atttgtacat aagcttgttc
     6181 attggttatt ctaatccaac acattgctgc tgcacaaagt gctgcacaat accatgaata
     6241 catgcaagat gccgagaggt ataatttgca aatacgcaca gcaggagatg ataaagtaag
     6301 agcaagccat gcagagctaa atggcattac actacctgcc aaccacgaat attgggacac
     6361 cacatggaca ccctttgact ggcgttgtag atgcagaata atacaggtat tataaagcaa
     6421 atatactacc accgatgatg gcattgccaa tgcagcagca cagcaagcca tacccgaagt
     6481 gtttaggtac aaccctggta aacagggtgt tatttttcct gaaaaacacc cttactatgg
     6541 tattagtaaa aatgctttgg aggtagttaa ggctatgcag caagtggagc aaagcaaaag
     6601 aattgattta aacaaataca taaaaaacaa tgtaactgct aaaattgtta gagatgtgat
     6661 gttggaatat tctaataact ttcctgaaaa ctttttaaat gggttagaaa aaataaaatt
     6721 tacatcctct caaagttata tgatgcaaca caccattaat gtaaataata taacaagaaa
     6781 acgtgtaggc aaaagcacta tttcaattag caatcaaaca ttgagaaatg gttataaccc
     6841 agctaaagag tttattgatg ggctggcttc aattaaacaa ggacgtgagc taacatttaa
     6901 ccaagagtat gcttttgaat caatgtggca tgaaatacta catgccaaaa ctgcaagcca
     6961 gcaccacaaa ttaagtaaca ggcaaaaaga acacatggaa acagtaaacc aatttgtggc
     7021 aaggcatacc tacccaaaat ttattgaagc atttgatggc aaagcctcca accaaatgaa
     7081 atattagata atggatatgg ctataaagaa tggataacca aatttagaaa caagttggct
     7141 aaaaaaggaa ttgacgaaaa gaaggcaatt gattttttag agccacattt aatgaaagat
     7201 tacagcagcc tggggcaaaa gatagatgag ttattggggg attaatacat catcaaaacc
     7261 aagtaaaaac tcttggtgta gttcgggtat tttagcccaa tattcattgg ctttttgtgg
     7321 ctgattacga tctgcaaaca gtatagccaa atcggcaata atcatatcat tgcttgtgtt
     7381 ttgcttatac tcattttccg ttttatagaa taagccaacg agttcttgct ctgttgggtt
     7441 ataatcaaaa atagtatcca taaagtaaaa ttactaaaaa tgcaacaatt tgaaactttt
     7501 tttaaaccta ttttaaaaga cttaaaagta gagcttaccg atgagtttga cagaaatttt
     7561 gaacgtaagg ctttttttta ttttgatatt tgtgataaag acttactgca atgtgcagat
     7621 gctgtaatac gattgagagc ataatattta tttcaacaga aaaaatttga tgaaatatat
     7681 tttcatttta cgaatgtagg tgtatttaga tggaatgatt ataaaaaagt aataagacca
     7741 tagctaatta aaaatttcat acaatgtatt tattcattaa aaataaaaaa ggcatttcaa
     7801 attgaaatac cttttaaata atttatttaa taaacaacaa gtaatataaa attattcagc
     7861 agttaaacta gcacataaat actcttgatt tagttttgca atatagctga tagaaatttc
     7921 tttagggcaa gttgcttcac aagctcctgt attggtacaa cttccaaagc cttctttatc
     7981 catttgtgcc accatactta aaactctact atttctttct ggctctcctt gaggtaataa
     8041 agctaaatga gaaactttgg ctgataaaaa taacattgca ctgctgtttt tacaagcagc
     8101 cacacaagct ccacaaccta tacacatggc agcagcaaag cttgcatctg ccttatcttt
     8161 atttattggc aaagcatttc cgtccactgc attaccagta ttaacagata tataaccccc
     8221 tgcttgtata attctatcaa aagcagttct atccacaact aaatctttaa taaccggaaa
     8281 agcattactt ctccaaggtt ctacaacaat tgtgtctcca tctttaaaag ccctcatgtg
     8341 taattggcag gttgtatttc cttgccatgg gccatgtggt ttaccattaa tatacattga
     8401 gcacattcca caaatacctt ctcggcaatc atggtcaaat gctattggtt catctccttc
     8461 tgcaattaac ctttcattca atacatcaaa catttctaaa aaactcattt ccgatgaaat
     8521 gttttctact ctgtacatct taaattcacc tcttgttttt gaattttttt gacgccaaac
     8581 ttttaaattc aaattcatgt tataatgttc cataatatgc tatagtttat taattctatt
     8641 aattttaatc aataaaaaat tactcttttt ttgttttaat acaatctatc attatttcat
     8701 attcttcttg tctattacta tctattctaa tatttttaaa ctttaagtta gataatgtta
     8761 tattctgaat atacattttc atttgaatac ctgttttaat attttgaaca tttaaaactg
     8821 aatcattata tttagaatat ttgtaagtat ctgttattaa actatcttca atataaaata
     8881 tactatcctt attaaattta ataaactgat tatttattga atggcaaaat gttcctctta
     8941 acgtatctct gctatccatc gtaaaatctg tttcacaaat aaatttaact tcccaaagac
     9001 ctattattgt tggtttttct acatttttgt tttcatttac attgggttgt tgattacaac
     9061 ctaaggtaaa taatagcaca aaaaaaacaa caactatttt aaccataact tctataaact
     9121 atttatagtt tctttgcgtt ggtttaatta cttcatactc taaagcttct ttatgtaatt
     9181 tccaattgct ttctccttca aattcccagg ctgctacata cataaactct gcatcatttc
     9241 ttaaagcttc tccttcttcg gtttggcttt cttctctaaa atggccacca caactttctt
     9301 ttcttaataa tgcatcttta cacattagtt ctccaagttc taaaaagtct gcaaccctac
     9361 cagctttgtc taattctgga ttaaattcat caatatctcc cggtattctt acatcattcc
     9421 aaaattcttt tcttaattgt tgtatttcta caatagcttc ttgtaaacct ttttcatttc
     9481 ttgccattcc gcatttatcc cacattattt tacctaaacg tttatgaaag gtttctaccg
     9541 atgtgttgcc cttaatattc atcaaggttc ttattctgtc tcctacttct ttctctgcat
     9601 ttacaaatgc ttcatgatcg gtagatatag gtttagagta tatttcatct gccaaataat
     9661 taccaattgt atatggtata acaaagtatc catctgccaa accttgcatt aatgcactag
     9721 ccccaagcct attagctcca tgatcgctaa aatttgcttc gcccaaacaa tacattcctt
     9781 ttacacttgt cattaattca taatcaaccc ataaaccacc cattgtatag tgtactgctg
     9841 ggtaaattct cattggtact tcataaggat tttctccagt aattttttca tacatatcaa
     9901 acaggtttcc atatttttct ttaactacct gttttcctaa tgtaataatt tcagcactgc
     9961 tagcatcttc tacttctctg ccatgttttg taatttcaat tttaccatat ctttcaattg
    10021 ccgaggcata atctaaataa acagcttgtt tacttgtacc tacgccatag cctgcatcac
    10081 atctttcttt agcagcacgg cttgcaacat ctcttggaac aaggttacca aatgcaggat
    10141 atcttctttc taaataataa tctctttctg attctggtat ttcattaggt tttctattat
    10201 ctccctgatt ttttggaacc caaattctac catcattcct caacgattcg gacattaagg
    10261 ttagtttact ttggtggtct ccactaactg gaatacaagt tggatgaatt tgtgtaaaac
    10321 aaggatttgc aaataatgca ccagctttat gtgccttcca tgcagcagta acattgctac
    10381 ccattgcatt ggtgcttaaa taaaatacgt tgccataacc accagtacaa agtaaaacag
    10441 catttccaaa atgtcgttgc aacttaccag aaattaaatc tcttgcaata atacctacag
    10501 cttttccgtc aatggtaacc acttctaaca tttcgtggcg gttatacatt tttacatttc
    10561 ccaaagcaac ttgtctttct aaagcttggt aagcccctat caataattgc tgaccagttt
    10621 gcccagcagc ataaaatgtt ctttgcactt gcgtaccacc aaaagaacga ttacttaata
    10681 aacctccata ttcccttgca aagggtacac cttgagcaac acattggtca ataatattag
    10741 cacttacttc tgctaaacga t
//
LOCUS       DAOQPK010000057        10678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_3219_length_10678_cov_4.168785, whole genome shotgun sequence.
ACCESSION   DAOQPK010000057 DAOQPK010000000
VERSION     DAOQPK010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10678
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3219_length_10678_cov_4.168785"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /gene="pgi"
                     /locus_tag="PLU36_04265"
     CDS             <1..745
                     /gene="pgi"
                     /locus_tag="PLU36_04265"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793090.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HMZ45995.1"
                     /translation="HFKTENHSKNIPVIMALIGIWYTNFFNAKSEAILPYDQYMHRFA
                     AYFQQGNMESNGKNTDRNGNEINYATGPVIWGEPGTNGQHAFYQLIHQGTQMIPCDFI
                     APVKSHNPLSDHHTKLLSNFFAQTEALMNGKTLNEAKEELIKAGKNEEEIKKLLAFKV
                     FNGNKPTNSFLIKKITPFTLGQLIALYEHKIFVQGVIWNIFSFDQWGVELGKQLANKI
                     LPELNNKEIVTCHDSSTNGLINWYKNNCS"
     gene            769..1485
                     /locus_tag="PLU36_04270"
     CDS             769..1485
                     /locus_tag="PLU36_04270"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HMZ45996.1"
                     /translation="MANSFFKFKQFTINQDACAMKVTTDACLFGAWCANLLNSVKPNN
                     ILDIGSGTGLLSLMLAQKSAAAIDSVEININAYNQAKQNIESSSFKSKISVHHSSIQQ
                     YSTTKKYDFIICNPPFFEQDLLSNNIAKNIAMHHTTLQLTTLVQCINNLITENGFFAV
                     LLPFHRTNYFEELTPNFKLLNKTIVQHSAKHPPFRTMFLFSKAQANNLYEECICIKEI
                     SLTQYTPAFITLLKPYYLYL"
     gene            1527..1928
                     /locus_tag="PLU36_04275"
     CDS             1527..1928
                     /locus_tag="PLU36_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ45997.1"
                     /translation="MKYSQLIGILAAIAIIGVCYMPWVYVPSINVTLSGINGKVNEQL
                     TFGRQVIPHTALSLLLILFFLIPKIWAKRTNLFIGFISLSWAIKNYIIFSMCRQTICP
                     DKKAGLYLLIILAFIVQISTLLPKLTVKQKD"
     gene            2059..4116
                     /locus_tag="PLU36_04280"
     CDS             2059..4116
                     /locus_tag="PLU36_04280"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HMZ45998.1"
                     /translation="MKKIYPGLLAVVLFYTLTANAQVNYTYNAYQSTYVHLEDGITPA
                     LDNPTPVGYYEDDEGFANQIPIGFTFNFNNTNYTHLNINVNGFVTFGSNFTMNVGSRY
                     STNNLMNGPMQENIRPIIAPLWDDLRLKNNKCLSYAVKGNAPNRIFIVQWDSASWNYN
                     LLDPAISFQMILHEGTNKVQFIYKALEGKLSDASASVGIATCSSCTGSFLSVTSIDKN
                     AQISGLEENKYINSLPTTGTLIEFEPGKNELPSNVMVNAYNTHEITLSWNTSAIAEYD
                     YAITTSPLQPLTFSTTNTPSATLQNLEKGKQYYLHVRTSTAAGKSGWVSIPAKTASYT
                     TLPYVEKFEGISIPNNPEAIHIANPLGGNSWQTIAVPGVPAFNYAISYKGDNINNADA
                     WFMLPAMQLTGGLTYRLQFKYRASDSLGGDQKLEVKIGKMMNNGMIGWQTIYKNVKIN
                     QLKFQDISKLFAAPTSDEYFIAFRCFSEKSNTAIWIDDIDVSKVKPLPIKMIAFSGFR
                     EGFVNKISWQTSAEFNNSSFELQRSSDGKNFTSINKIETKANNGTSSTTLNYSLIDNT
                     PNTVDYYRLKVTDKESNDFYVQDIIKISSELPFLISAQKTYPNPATNVLNTVVYSKYK
                     TVGIIKIMDNYGHIVKAYSINIEAGDNIIQTDISQFNRGMYFVKVENKIGKAGNAKSF
                     IKQ"
     gene            4272..5540
                     /locus_tag="PLU36_04285"
     CDS             4272..5540
                     /locus_tag="PLU36_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ45999.1"
                     /translation="MKNLIICLVCFLYIPFANAQINYSFTTSGASFDMVTNGTTPMFT
                     GNGTDPLADEGFVNYIPIGFSFSYNNYSNTYNEVAISSNGFISLTELSDSYVINNLNN
                     GASGERPIIAPLWDDLDLSLTSNLTYTTTGSAPNRVFTVQWLNVNWGFGSTSAAISFQ
                     LKLYETSNWIEFIYRPEAGFATSPSASIGLTALGTGTNNFVSIGGFSLTPSVSVSSES
                     NNITAKPNNVIKYTFKPGVLPVKMEYFTVTKDKEKHILKWQTVQETNHKQFNIQRSNN
                     GVNFSNIASQPSFESNGNSKTYLYADTKPLIGNNYYRLQQIDVDGKTNYSSIVYIKNV
                     NNNWASLNIYPNPVVNNLQVNINTHKAEKVNISVTDISNTVLLHSVYYTQPSENLYIL
                     NVQNLAKGTYFIKVYNSSTEQFIVQKFIKN"
     gene            complement(5537..6538)
                     /locus_tag="PLU36_04290"
     CDS             complement(5537..6538)
                     /locus_tag="PLU36_04290"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HMZ46000.1"
                     /translation="MKFRYEFNIKSLPKAINHNHKIFLIGSCFTENIGEKLKKYKFSV
                     LENPNGILFNPVSVSEALINIIENKQYVANDLFEYNEAWHSWQHHSRFSGITVEETLE
                     KINQSTATAHQYLKQADYLLITLGSAWIYTLTNKANNATVGTVAANNHKAPADWFERK
                     LPDVAQVITVLVTMLNKLGSINPNIQVIFTISPVRHLREGAIENNRSKAALISAVHYL
                     IDNLPKLYYFPAYELVIDDLRDYRFYAEDLVHPNYAATDYVWSKFVQSCMQKETIELM
                     EIIKEINLAFQHKPFNAHSNQHTQFLATYFAKTKALTKKYANLDFDKELAYFKNLND"
     gene            6687..7115
                     /locus_tag="PLU36_04295"
     CDS             6687..7115
                     /locus_tag="PLU36_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46001.1"
                     /translation="MKKQIIGIVLIVAMVSFSGCKKAIEKIKEQEIVNAITNGKWVVS
                     KFVEGTTTTTTDFAGWETIYYENGTFTSSKTGETNQTGTWAGNASNWTFTITFNATPP
                     TPLNKLGGVWTVTRAVSTDKGSYAKTEAGVNYELEMTKMP"
     gene            complement(7189..7851)
                     /locus_tag="PLU36_04300"
     CDS             complement(7189..7851)
                     /locus_tag="PLU36_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ46002.1"
                     /translation="MSNFLLSAKNITKKYNELPILKGIDIAIESNQIVSIVGSSGAGK
                     STLLHILGTLDKADSGEIWLNDIRVDLLSGKKLAAFRNMHIGFIFQFHHLLPEFSAIE
                     NVYIPAWIAKRKTTEVKKEAAQLLDILGLSNRLNHKPNQLSGGEQQRVAVARALINKP
                     SIVFADEPTGNLDSKNAKELHHLFTQLCTNLHQTFLIVTHNEELASLSNRVLHMKDGY
                     MV"
     gene            complement(7971..9152)
                     /locus_tag="PLU36_04305"
     CDS             complement(7971..9152)
                     /locus_tag="PLU36_04305"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HMZ46003.1"
                     /translation="MNTRTVYIVSAVRTPLGSFGGILKDLSATQLGAVAISAAVEKAG
                     LEPEQIQEVIMGCVIQANLGQAPARQAAKFAGLPDNVVATTVNKVCASGMKAIAQAAQ
                     SILLGDADIVVAGGMESMSNVPFYSPNLRWGNKYGNVSMIDGLAKDGLTDVYFNGAMG
                     NFADATAKECNITREEQDAFAIESYTRSQNAWKEGRFANEVVPVVIESKKGNIVIDKD
                     EEPFNVKFDKIAGLRPAFNKDGTVTAANASTMNDGAAALVLMSKEKADELGIKPLAII
                     NSYADAEQAPEWFTTTPSLAVPKAVTKAGLTLNEIDFFELNEAFSVVGIVNCNKMNIP
                     FNKVNVHGGAVSIGHPLGCSGARIIVTLINVLKQNNGKIGAAGICNGGGGASAMVISL
                     P"
     gene            complement(9238..10254)
                     /locus_tag="PLU36_04310"
     CDS             complement(9238..10254)
                     /locus_tag="PLU36_04310"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HMZ46004.1"
                     /translation="MKKIGTIIKYVFFLSLGLLLLWLSFHNIRKNPEQWMQFKQTIKS
                     ANYLLFLPVFFIITASHAVRALRWRVLIEPMGYKVSFLNSFFAVMIGYMANMAIPRLG
                     EVLKCTILAKYEKVPAEKIVGTMVAERAIDALTLGLVFLLALIFQFNVVNNAYNELKI
                     IMAGKPNEAISSTKIIILVTILIIAIVLFIWLKSTNKLKKIKSSILKIIKGIMEGVTS
                     TFKLKQKKQFFFYSALIWLMYLAGTWLGFYATQGTTGLGVDAALSCLAFASIGMIITP
                     GGIGAYAALLAIVLTLQNPPIPNAIGYANGTLQWFAQCIIVLIVGFICIIFLPLYNKS
                     KKVN"
     gene            complement(10371..>10678)
                     /locus_tag="PLU36_04315"
     CDS             complement(10371..>10678)
                     /locus_tag="PLU36_04315"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HMZ46005.1"
                     /translation="PDLKEEDILLLEQEIDNMNKELPVMKSFILPGGHVVVSTTHIAR
                     CVCRRAERICVAMQQEEMFISPIVIKYINRLSDYLFMLARYTAQQLNVQEIPWKARV"
BASE COUNT         3824 a         1716 c         1514 g         3624 t
ORIGIN      
        1 ccattttaaa accgaaaatc attcaaaaaa tattccagta ataatggcat taattggtat
       61 atggtacact aattttttta acgctaaaag cgaagccatt ttaccttacg accaatatat
      121 gcacaggttt gctgcctatt ttcagcaagg aaatatggaa agtaatggaa aaaatactga
      181 tagaaatgga aatgaaatta actatgcaac aggcccagta atatggggcg aacctggcac
      241 caatggtcaa catgcatttt accaattaat tcatcagggc acacaaatga taccatgcga
      301 ttttattgca cctgttaaga gccataaccc tttaagcgat catcatacaa agcttttaag
      361 taactttttt gcacaaacag aagctttaat gaatggtaaa actctaaatg aagcaaaaga
      421 agaactaata aaagcaggaa aaaatgaaga ggaaataaaa aaattacttg cttttaaagt
      481 ttttaatggg aataagccaa ccaattcttt tttaattaaa aaaattactc cattcacttt
      541 aggtcagctc attgctttat atgaacataa aatttttgta caaggagtta tttggaacat
      601 atttagtttt gaccaatggg gtgtagaatt aggcaagcaa ttagccaata aaattttacc
      661 agaattaaat aataaagaaa tagttacatg ccatgatagt agcaccaatg gtttaataaa
      721 ttggtacaaa aataattgta gttaatactc tattagtgta aggcttttat ggctaattca
      781 tttttcaaat tcaagcagtt taccattaat caagatgctt gtgctatgaa ggtaacaaca
      841 gatgcttgtt tatttggagc atggtgtgca aatttattaa actcagtaaa gccaaataat
      901 attttagata ttggttcagg cacagggtta ttaagcttaa tgctggctca gaaatctgct
      961 gctgcaatag attcagttga aataaatatt aacgcttata atcaagctaa acaaaatatt
     1021 gaatcttcta gttttaaaag taaaattagt gtacatcatt cttccattca acaatacagc
     1081 actacaaaaa aatatgattt tataatctgc aaccctcctt tttttgagca agatttatta
     1141 agtaataata tagctaaaaa tatagcaatg caccatacaa cattacagct taccacttta
     1201 gtgcaatgta ttaataactt aattacagaa aatggttttt ttgctgtgtt attgcctttt
     1261 catagaacaa attattttga agaattaacc ccaaatttta aattattaaa taaaacaatt
     1321 gtacaacatt cagccaaaca tccaccattt agaactatgt tccttttttc taaagcacaa
     1381 gcaaacaatt tgtacgaaga atgtatttgt attaaagaaa ttagtttaac acaatacact
     1441 cctgctttta ttaccttact taaaccttat tatttatatt tataattcac aaaaaaaata
     1501 tatttgcgac ctatttattt ttaattatga aatattctca gttaataggc atattagcag
     1561 ctatagcaat aattggtgtt tgttatatgc cttgggtata tgttcctagt attaatgtaa
     1621 ctttatctgg aataaatggt aaggtgaatg agcagttaac ttttggccgc caagtaattc
     1681 ctcatacagc attaagttta ttgttaattt tattcttttt aattccaaaa atttgggcta
     1741 aaagaaccaa tttatttata ggttttataa gtttaagttg ggcaataaaa aattatatca
     1801 ttttttcgat gtgtaggcaa acaatatgcc ctgataaaaa agctggttta tacctattaa
     1861 taattttagc ttttattgtt caaatttcta cactattgcc aaaacttaca gtaaaacaaa
     1921 aagattaaag taagaagaaa tctaccttgt gcacattgtg taaataattg tattcatatt
     1981 tttttgcttc taacacaata aaataattaa ttattagttt attagttagc aaatatgctt
     2041 taatccaata tcaatgctat gaagaagatt tacccaggtc ttttagctgt agttttattt
     2101 tataccttaa ctgcaaatgc acaagtaaac tatacttaca atgcttatca aagtacttat
     2161 gtgcatttag aagatggtat tacaccagct ttagataatc caacacctgt aggttattac
     2221 gaagatgatg aaggttttgc taatcaaata cctattggct ttacttttaa ttttaataat
     2281 acaaactata cacatttaaa tataaatgta aatgggtttg taacatttgg gtcaaacttt
     2341 actatgaatg ttggtagccg ttacagcaca aacaatttaa tgaatggccc aatgcaagaa
     2401 aatatcaggc caattattgc ccctctttgg gatgatttaa gactaaaaaa caataaatgt
     2461 ttaagctatg ctgtaaaagg aaatgcacct aacagaattt ttattgtaca atgggatagt
     2521 gcatcttgga attataattt attagatcca gcaatttcct ttcaaatgat tttacatgaa
     2581 ggaactaata aggttcaatt tatttataaa gcattagaag gaaaactatc agatgctagt
     2641 gcttctgtag gtattgcaac ttgtagttct tgcacaggaa gttttttatc ggtaacatca
     2701 attgataaaa atgcacaaat aagtggatta gaagaaaaca aatacataaa ttctttacct
     2761 acaacaggca ctttaataga gtttgaacca ggtaaaaacg aattaccatc taatgtaatg
     2821 gtaaatgctt ataatacaca tgaaataact ttatcttgga atacttctgc aatagcagaa
     2881 tatgattatg caattactac ttctccttta cagcctttaa ctttttctac aactaatacg
     2941 ccttctgcaa cacttcaaaa cttagagaaa ggaaagcaat attatttaca tgtaagaaca
     3001 agcactgcag caggtaaaag tggatgggtt tcaatacctg ctaaaactgc atcttatact
     3061 accctacctt atgtagaaaa gtttgagggt atttctattc ctaataatcc tgaagcaatt
     3121 catattgcca atccattagg tggcaattct tggcaaacaa ttgctgttcc aggtgtgcct
     3181 gcttttaatt atgccatcag ttataaagga gataatatta ataatgccga cgcatggttt
     3241 atgttgcctg ctatgcaatt aacaggcgga ttaacttatc gtttacaatt caaatataga
     3301 gctagcgatt ccttaggtgg agatcaaaaa ttagaagtaa aaattggaaa aatgatgaat
     3361 aatggaatga taggttggca aactatttat aaaaatgtaa aaattaatca actaaaattt
     3421 caggatattt ctaaactttt tgcagcacct accagcgatg agtattttat agcttttaga
     3481 tgtttttctg aaaagagtaa cactgccatt tggattgatg atattgacgt aagtaaagta
     3541 aagcctttac caataaaaat gatagctttt agtggcttta gagaaggttt tgtaaataaa
     3601 atttcttggc aaacctcagc agagtttaac aatagcagct ttgaattaca aagaagttcc
     3661 gatggtaaaa attttaccag cataaacaaa atagaaacca aagcaaataa cggaacaagt
     3721 tcaacaacat taaactatag cttaatagat aatacaccaa acacagttga ttattatagg
     3781 ttgaaagtta ctgataaaga aagtaatgat ttttatgtgc aagatattat taaaataagt
     3841 agcgaattac cctttttaat tagtgcacaa aaaacttatc ctaaccctgc cacaaatgtt
     3901 ttaaatactg ttgtttactc aaaatataaa acagtgggta taataaaaat tatggataat
     3961 tatggccata ttgtaaaagc ttatagtatt aatattgaag caggagataa cattattcaa
     4021 acagatatta gccaatttaa tagggggatg tattttgtga aagttgaaaa taaaattggc
     4081 aaagcaggca atgcaaaatc ttttataaaa caataaaatc tttaggagcc acttgaaagt
     4141 atcgctaaat gcatctacct gatgaaaaag gcaagaagca aaaagctcaa ctcttttcaa
     4201 taaatgtttt gatagctcat ttattgctgt tgttttcttg tggcttctaa aactaattaa
     4261 ctataaccaa tatgaaaaac ctaattatct gcttagtatg ctttttatac ataccttttg
     4321 ctaatgctca aattaattat tcctttacaa cttctggtgc tagttttgat atggtaacta
     4381 atggaacaac accaatgttt actggtaatg gaactgaccc tttggctgat gaaggatttg
     4441 taaattatat tccaattggt ttttcattta gctataataa ttatagcaat acttataacg
     4501 aagtagctat ttcatcaaac ggttttatta gccttactga attaagtgat agctatgtaa
     4561 ttaacaattt aaataatggt gcaagtggcg aaagaccaat aatagcacca ctttgggatg
     4621 atttagattt atcactcacc tctaatttaa cttataccac aacaggctct gcacctaaca
     4681 gagtatttac tgttcaatgg ctaaacgtta actggggttt tggttcaact tctgcagcca
     4741 tttcttttca actaaaatta tacgaaacaa gtaattggat tgaatttatt taccgcccag
     4801 aagctggttt tgctacttcc ccatctgcat ctattggatt aactgcttta ggaaccggaa
     4861 ctaataattt tgtttctatt ggtggttttt ctttaacacc atctgttagt gtaagcagcg
     4921 aatcaaacaa cattaccgca aagccaaaca atgtaattaa atacacattt aagcctggtg
     4981 tattaccagt taaaatggaa tatttcactg ttactaaaga taaagaaaag catattttaa
     5041 aatggcaaac tgtgcaagaa acaaatcata aacaatttaa tattcaacgt tctaataatg
     5101 gtgtaaactt tagtaacata gcatcacaac caagctttga aagtaatgga aatagtaaaa
     5161 cctatttata tgcagatact aaaccattaa taggtaacaa ttattacaga cttcaacaaa
     5221 tagacgtaga tggaaaaaca aattactctt ctattgtgta tataaaaaat gtaaacaata
     5281 actgggcttc attaaatatt tatcctaacc cagttgtaaa taacttacaa gtaaatatta
     5341 acacacataa agcagaaaag gtaaatatta gcgttacaga tatttctaac acagttttat
     5401 tacactccgt ttattataca cagccatcag aaaacttata cattttgaat gttcaaaatt
     5461 tggctaaagg aacctatttt attaaagttt acaattcatc cacagaacaa ttcattgttc
     5521 aaaaatttat aaaaaattaa tcgtttaaat ttttaaaata tgctaactct ttgtcaaaat
     5581 caaggttggc atattttttt gttaatgctt ttgttttagc aaagtaagta gcaagaaatt
     5641 gtgtatgctg attagaatgt gcattaaatg gcttatgctg aaatgccaaa ttaatttctt
     5701 taattatttc cattaattct atggtttctt tctgcataca ggattgtaca aatttgctcc
     5761 atacataatc agtagcagca taattaggat gtactaaatc ttctgcataa aacctataat
     5821 ctcttaaatc atcaattact aattcatacg caggaaagta atatagcttt ggcaaattat
     5881 caattaagta atgtacagca gagataagtg ctgccttgct tctattattt tcaattgctc
     5941 cttctcttaa atgccttact gggctaatag taaatattac ctgaatattt ggatttatac
     6001 ttcctaactt atttaacata gtaacaagta ctgtaataac ttgtgcaaca tctggcaatt
     6061 ttctttcaaa ccaatcggca ggggctttat gattatttgc tgcaactgta ccaacagttg
     6121 cattatttgc tttgttggtt aaagtataaa tccatgcact acctaaagta attagcaaat
     6181 aatctgcctg ctttaaatat tggtgtgcag tagctgtaga ttggtttatt ttctctaatg
     6241 tttcttctac agttatacca gagaatctgc tatggtgttg ccaactatgc catgcctcgt
     6301 tatattcaaa caaatcatta gcaacatatt gtttattctc aataatatta attaatgcct
     6361 ctgaaacact tacagggtta aataaaatgc catttggatt ttctaatacg gagaatttat
     6421 attttttcaa cttttcccct atattttcag taaaacaaga gccaattaaa aaaattttat
     6481 ggttatggtt aattgcttta ggcagcgatt taatattaaa ttcatatcta aatttcatat
     6541 agcaaatata ttatgtagtg ttgcctatta gcaataaatt aaacttaatt tcgttttatt
     6601 accatccaaa aaataaaaaa atttgctgga gttatttaat aaatatctta gcaaaataat
     6661 aatattacaa cttaatataa atatttatga aaaagcaaat aattggtata gtattaatag
     6721 ttgcaatggt aagttttagt ggctgtaaaa aagctattga aaaaattaaa gaacaggaaa
     6781 ttgtaaatgc cattactaat ggtaaatggg tagtttctaa atttgtagaa ggcactacta
     6841 ccaccactac tgactttgca ggatgggaaa caatttatta tgaaaatggc acatttacaa
     6901 gttctaaaac tggtgaaaca aatcaaacag gtacttgggc aggaaatgct tctaattgga
     6961 catttacaat tacttttaat gcaacaccac caacaccact aaataaatta ggaggtgttt
     7021 ggacagtaac gagagcagtt tctacagata aaggcagtta tgcaaaaacc gaagctggtg
     7081 taaattatga actagaaatg acaaaaatgc cttaaaggct atattaagtt ttaaaaaata
     7141 gatgataaag ttttgtagta ttataccaaa taaataactt aaaacaattt aaaccatata
     7201 tccatctttc atgtgcaata ctctattgct taaagatgct aattcttcgt tatgggtaac
     7261 aattaaaaag gtttgatgaa gatttgtgca tagttgtgta aataaatgat gcaactcttt
     7321 tgcattttta ctatctaagt ttcctgttgg ctcatctgca aaaactattg atggtttatt
     7381 aattaatgct cttgctactg ctacacgttg ctgttctcct ccactaagtt gattgggttt
     7441 atggtttaac ctattgctta aacctaaaat atctaataat tgtgcagcct cttttttaac
     7501 ctcggttgtt tttctttttg caatccatgc cggaatataa acattttcaa tagcagaaaa
     7561 ctcgggtaat aaatgatgaa actgaaaaat aaaaccaata tgcatatttc taaatgcagc
     7621 taatttttta ccacttaata aatcaaccct aatatcattc aaccatattt cgccactatc
     7681 tgctttatct aatgttccta aaatatgcag caaagtgctt ttaccagcac cactactacc
     7741 aactatacta acaatttggt tgctttctat tgcaatatca atacctttta aaatgggtaa
     7801 ttcgttatat tttttggtta tattttttgc agataataaa aaatttgaca taaattattt
     7861 caataatggt atagaaatgt ataacaactt tgtttaaaaa taatgaatat taaaaactaa
     7921 aaagcaatct aaataaagat tgcttttttc aaaaaaatat tttaaaataa ttatggtaaa
     7981 gaaataacca ttgcactagc ccctcctcca ccattacaaa tacctgcagc tcctatttta
     8041 ccattatttt gtttcagtac atttattaat gttacaataa ttctggctcc actacaacct
     8101 aaagggtgcc caatacttac agcaccacca tgtacattta ctttgttgaa aggtatattc
     8161 attttattgc agtttacaat accaacaaca ctaaaagctt cgttcaattc aaaaaaatct
     8221 atttcgttta aggttaaccc tgctttagtt acagcttttg gtacagccag gcttggtgta
     8281 gtggtaaacc actcaggtgc ttgttcagca tcggcataac tgttaataat tgctaaaggt
     8341 ttaatgccta attcatctgc tttttcttta ctcattaaaa ctaatgctgc agctccatca
     8401 ttcatggtgc tagcatttgc agcagttact gtaccatctt tattgaatgc tggtctaagg
     8461 cctgcaattt tatcaaactt aacattaaaa ggttcttcat ctttatcaat aacaatattt
     8521 ccttttttac tttctattac aacaggtact acttcattag caaatcttcc ttccttccat
     8581 gcattttggc tacgggtata actttcaatt gcaaaagcat cttgttcttc tcttgttata
     8641 ttacattctt tagctgttgc atcagcaaaa ttgcccattg caccattaaa atatacatct
     8701 gttaagccgt ctttagctaa cccatcaatc attgaaacat ttccatattt atttccccaa
     8761 cgtaaattag gagaataaaa aggcacatta ctcatacttt ccataccacc tgcaacaact
     8821 atatctgcat ctcccaataa aatactttgg gcagcttgtg caattgcctt cattccgctt
     8881 gcacaaactt tatttacagt tgtagcaacc acattatctg gtaatcctgc aaactttgct
     8941 gcctggcgtg ctggtgcctg gcctaaattt gcctgaatta cacaacccat tattacttcc
     9001 tgaatttgct ctggctctaa gcctgctttc tctacagcag cactaattgc aactgctcct
     9061 aattgtgtag cagataaatc ttttaaaata ccgccaaaac tacctaatgg tgtacgtact
     9121 gcactaacaa tataaactgt tctagtattc atttataata aatttaagtg gcaaatttac
     9181 acaaaaccta acaaatgtta gtatgatttt ttaaaggtta taaaaaaaga aaatatttta
     9241 attaaccttt tttgatttat tgtataaagg caaaaatatt atacaaataa agcctacaat
     9301 taatacaata atacattgtg caaaccattg taaagtacca ttggcataac ctattgcatt
     9361 gggtattggt ggattttgta gagttaaaac tattgccaat aaagctgcat aagcaccaat
     9421 accaccaggt gtaataatca tacctatgct agcaaatgcc aagcaagata aagctgcatc
     9481 aacacctaag ccagtagtac cttgggttgc ataaaagcca agccaagtgc ctgctaaata
     9541 cattaaccaa attagtgctg agtaaaaaaa gaattgcttt ttttgtttta atttaaaggt
     9601 agaagttacc ccttccataa tgcctttaat aatttttaaa atacttgatt ttatcttttt
     9661 taatttattg gttgatttaa gccagatgaa taatacaata gcaattatta atatggttac
     9721 taatattatt attttagtac tactgattgc ttcgtttggt ttaccagcca taataatttt
     9781 tagctcgttg taggcattat taactacatt aaactgaaaa ataagagcta ataaaaaaac
     9841 taagcctaag gttaatgcat ctatagctcg ttctgcaacc atagtaccaa caattttttc
     9901 agcaggaact ttttcgtact ttgctaaaat ggtacatttt aaaacttcgc ctaaacgggg
     9961 tatagccatg tttgccatat agcctatcat tacagcaaaa aaggaattta aaaaagaaac
    10021 tttataaccc atcggctcta ttaaaactcg ccaacgcaaa gctcttactg catgactagc
    10081 agtgatgata aaaaacacag gaagaaataa taaataattg gcagatttaa ttgtttgttt
    10141 aaactgcatc cattgttcag gattctttct gatattatga aaacttaacc acaataaaag
    10201 caagcctaac gataagaaaa atacatattt tattatggtg cctattttct tcatatacta
    10261 caaaattgca attattgatt gatagttgca gtaaatttat actttaataa ttttattttt
    10321 tacaccgtat atagtaatga agaaaattat aatgagtatg ctactacaat ttatacccta
    10381 gctttccaag gaatttcttg aacatttaat tgttgtgctg tataacgtgc aagcataaat
    10441 aaataatcgc taagcctatt aatgtactta attacaattg ggctaataaa catttcttct
    10501 tgttgcattg ccacacaaat acgttctgcc cttctacaca cacatctagc aatatgtgta
    10561 gttgaaacaa caacatgccc accaggtaaa ataaaacttt tcataacagg taattcttta
    10621 ttcatattat caatctcctg ttctaacaat aaaatatctt cttcttttaa atcgggta
//
LOCUS       DAOQPK010000058        10658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_3232_length_10658_cov_4.285391, whole genome shotgun sequence.
ACCESSION   DAOQPK010000058 DAOQPK010000000
VERSION     DAOQPK010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10658
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3232_length_10658_cov_4.285391"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PLU36_04320"
     CDS             <1..522
                     /locus_tag="PLU36_04320"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HMZ46006.1"
                     /translation="SMSVATGTVNGEGLVIACMDFEFIGGSLGSVMGEKFSRAVDYCI
                     EHKLPYLVICKSGGARMMESAFSLLQLAKTSGKLSMLSDNQLPYISLLTDPTFGGISA
                     SFGMLGDLNIAEPGALIGFAGPRVIKETIKKDLPEGFQRSEFLLEHGFLDFIVDRKNL
                     KNKLAQLIKLLRS"
     gene            571..1104
                     /locus_tag="PLU36_04325"
     CDS             571..1104
                     /locus_tag="PLU36_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46007.1"
                     /translation="MQIFSASNAQSWIDNSGKNSLSIGASSGASFPVAIQGYTVGVAG
                     DVVLNYNTKIGTANLLWGYNFLFKPKTNNYSVNLIMHYAYGVGFSKRFLPSQKLFTAI
                     DITYNEAEEIISFMKNPKHTGIGFGLTTGYSFAMNKKSLWSVSANYHYAMLNNVNFSN
                     AGIRLHYSLPLVIMNKK"
     gene            1330..4005
                     /gene="gyrA"
                     /locus_tag="PLU36_04330"
     CDS             1330..4005
                     /gene="gyrA"
                     /locus_tag="PLU36_04330"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HMZ46008.1"
                     /translation="MEENTTQFPDQTNDNDRVVQVNIEEQMKTAYIDYSMSVIVGRAL
                     PDVRDGFKPVHRRVLYAMNELSNNSGKPYKKSARIVGEVMGKYHPHGDSAIYDTLVRM
                     AQDFTMRHTMVDGQGNFGTQDGDPPAAMRYTEVRLQKFAEAMLEDIEKDTVDFQSNFD
                     DSLEEPTVLPTRIPQLLVNGSSGIAVGMATNMMPHNLSEVIDGCIAYIHNKDITIDDL
                     LHFVKAPDFPTGGIIYGMEGVKQGLLTGRGRVVLRGKCHVETKQSGREQIIITEVPYQ
                     VGLDALTDKIGQLVNDKTIEGIAHVNNESNKREGIRIVIDLKRDAVANIIINQLYKFT
                     ELQTSFGINNVAISKGRPRIFNVKDLIVEFVDFRMDVVIRRSKFELKKAEDRAHLLEG
                     YLKVINDKDHLDLAISIIRGSATPDEAKKSLIERSIADNWYLPDNLFDEKAKIHYLQG
                     IGLSEKQAQAILELRLQRLTGLEREKIIEEFNDLMNTIVRLKDLLASEDLRYDLIKTE
                     LEEVKAKFGEPRKSEIQFLADEVNMLDFIKEEDVVITVSHLGYIKRTSATEYRQQKRG
                     GRGAIGSKTREEDFVEHLFVASTHDVMMFFTEKGRCYWLNVYEIPEGEKQSKGRAIQN
                     LIQIPPDDKIKAIIDVKDLDKKETLQNRYVVLCTKKGIIKKTDLEDFSRPRTTGINAI
                     TILEGDELLEAKLTDGNCEIMMAVKSGRAIRFNEEKVRATGRGAIGVTGIEVDETNDE
                     VVGMICVNKNDTDKTVLVISEKGYGKRTKLTDEDGEDVYRITNRGGKGVKTINVTEKT
                     GRLVGILDVNEKEDLIITCQSGITLRTSISTIKTAGRNTQGVILIRLDAEDAIAAISK
                     IEETEEETTTEVNDQSENNSPENTDNLNTNIENSN"
     gene            4016..5413
                     /locus_tag="PLU36_04335"
     CDS             4016..5413
                     /locus_tag="PLU36_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46009.1"
                     /translation="MKKIILLVACSISLVIANAQDKMYKKLQTLYIAAQYEEAKAEIE
                     KVMANPKAIDKAETWLWRTRIYAEIYFSEPLRKLNPGCGNIAFESFKKYEALESNYKM
                     LANPELSAWRWRPLDLIYVTSFNLGRSFFEEKKWDSSYNTFMKSVYMGDVIVKNNLRE
                     NGAKIDTVSVLYAAYAAQNGKMEAEATALYEKFADCKIGGKDFKDAYMYILLYASKTK
                     DKAKFNKYIAIAKEVYPNDDAWDDYEMEFITNTTTVEERIAYYDKEDAAGTLTARKYM
                     LFGQSFSEATKGEEAEKLDSLTKVRYDKKAVEAFKKAYQKDNKLGIAAYNAGLLYYNE
                     FVEYDDRISNNRRALQQLNSNKPVVKDPKKKAVADAEFKKQTDAIKKLNSDLEKPILE
                     TLDSCVVWLEIAYNNLKNGNMEDRTTLNCIKNSVKWLANAFMYKREKVKGKDTKAYDA
                     YDAKYNEYDKLYEKY"
     gene            5520..6224
                     /locus_tag="PLU36_04340"
     CDS             5520..6224
                     /locus_tag="PLU36_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HMZ46010.1"
                     /translation="MQLGTVYLIPTVLFEDELHCIPDYVVDTIKNCEIFFTENEKTTR
                     RFFKKLWKEMVIDNYQWHTIHKTEQAVVTTFLQHLKDGKNVGIVSEAGCPGIADPGQI
                     LVEAAQQINAIIKPLVGPSSILLALMASGMNGQCFKFNGYLPIDAMERKKKIKALEME
                     SLQQNCTQIFIETPYRNNQLINDVLSICNKQTKFCIAVNITSAAEKISTKSIESWKKN
                     IPDIHKQPAIYLLNAF"
     gene            6277..9690
                     /locus_tag="PLU36_04345"
     CDS             6277..9690
                     /locus_tag="PLU36_04345"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ46011.1"
                     /translation="MLKKILSTLLIIVGFKAFSQTPIQMSSQVNNSYTETFADIDNWV
                     FKTTSEDGTFTAGVGAMCWRGVAINATGTIPSALKITHASTFFQIPPTGNGGYSSGVY
                     KGNQKLEILSTGTTNNTSSIAIDFFMDFTNVNAGTLSFDWASINNSSGNRSSSLKVYA
                     TVDGVNYIEITNAQVLNFTNNTPTAGSVVNVPLPTIFNNSATASLRFYYHNGTGGSSG
                     SRPRLSIDNVHVTAVPTTPCTAPTMQPTNFTLGAVTHNSINFSFTSAVPAPHNYFILM
                     STNSTLTALPVNGITYNVGDALADATVIGITNNNTFTASNLDLSTTYYFFLFSVNNSC
                     TGGPLYLTVNPLNGSATTTAGALPCAAPTTQPTNLSFTSITTNSIKGSFVPAANTDEY
                     LIIRSLSPTFTGILNNGTTYNGGNILGNGMVVTRTAGNTFTANNLTNGTTYYFFVFGL
                     NNQNCTGGAAYNSTNPLTNNTSTVSLPPCVTPTNQPSQLQLSASNHYVNGYFVAAPSA
                     DGYLIVRTAIATLSVTPQNGINYNAGDGLGNGTIIQNNASTAFIDINLQPSTTYYYHV
                     FAKNSVCNGNINYLSVNPLTGNTSTTAIAVNNWYYGNLHSHSSHSDGNQDNLSLTPID
                     DYAYAKNSLCMDFLGISDHNHASAGMSISNWQPGLNAASAATTSNFLALYGMEWGVIS
                     NGGHVLVYGSNQLIGWETNNYNIYVPKSDYLGTPETTGTTGLFRAINNLGGNTFATFA
                     HPSFSDYNGIANISYSATADSALVGSAVASGIAYSTNTTYSNPPSSFGYIDFYNRMLS
                     KGYHIGPTMDHDNHYTNFGRSNNNRLVVVAPSISSNDFYSAMRNRNFYASEDCDTRVV
                     FTLNNKKMGSILKDTVAPAISVYAYDPTNPGFTPTIKLMFGIAGSGVNPIQIYSSNSS
                     TLNYSDYELPIGSNAYYYADIQIAGNRTITAPIWFTKEALLPITLLSFNAYLNKEFNV
                     VNLNWQTTNEISNNYFILEKSNNGVEFFRLDSVKAKNTSGINNYAIVDKQPFDNITYY
                     RLKQVDMDGKYSYSKIIAVQLNNLLNEFTIQPNPIYNQASVKIKSYKKENTILKIMDM
                     QGRVVLQQLLQLNIGTQQININCNSLQKGLYNATLFLNNQIITRKVVKL"
     gene            complement(9707..>10658)
                     /locus_tag="PLU36_04350"
     CDS             complement(9707..>10658)
                     /locus_tag="PLU36_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HMZ46012.1"
                     /translation="KVNENPNIGIKALISLAALEKSISINNLVFIVAPRINAAGRMDD
                     AKKAVQLFIETNVAKALEYAEMLHVDNKERKEADSSITEQALNLIKNNPTLINKKTTV
                     VYNETWHKGVVGIVASRLIETYYKPTIVLTKSGNLITGSARSVVGYNLYDAIYSCKNY
                     LTNYGGHFAAAGLSLLPEQLENFTNAFETVVSNTISPDLLIPEIIIDAELAFDEITTT
                     FYNIIMQMEPFGPNNMRPVFISRNVIDTGFSKIVKEQHLKISVKQNNIILNGIGFNMA
                     NKFSILEQNNQVDIVYTIDQNEWNGEKILQLKLIDLKPSK"
BASE COUNT         3988 a         1486 c         1820 g         3364 t
ORIGIN      
        1 agcatgagtg ttgctacagg tactgttaat ggcgaagggt tagtaattgc atgtatggat
       61 tttgaattta taggtggaag tttaggaagt gtaatgggag agaaattttc aagagctgtt
      121 gattattgta ttgaacacaa attaccttat ttagtaattt gcaaaagtgg tggagcaaga
      181 atgatggaaa gtgcttttag cttattacaa ttagccaaaa ccagtggtaa attatccatg
      241 cttagcgata atcaactacc ttatatttcc ttattaaccg acccaacatt tggaggcatt
      301 tctgcaagtt ttggaatgtt aggagatttg aatattgcag aacccggtgc tttaattggc
      361 tttgcaggtc caagagtaat aaaagaaaca attaaaaaag atttaccaga aggttttcaa
      421 agaagtgaat ttttattgga acatggtttt ttagatttta ttgtggacag gaaaaattta
      481 aaaaataaat tagctcaatt aataaaactt ttgaggagtt aatttatgca ttcaatcaac
      541 ggttacaagt tcctttttct tattctacta ttgcagattt tttccgcttc aaatgctcaa
      601 tcttggattg acaattctgg taaaaatagt ttatccattg gtgcttcttc aggtgcatcc
      661 tttccagttg caatacaagg ttatacagta ggcgttgcag gagatgtagt gctaaactat
      721 aatactaaaa ttggtactgc aaacttacta tggggttata attttttatt taaacccaaa
      781 acaaacaatt actctgtaaa cttaataatg cattatgctt atggtgtagg tttttcaaaa
      841 agattcttac catctcaaaa attatttact gcaatagata taacttacaa cgaagcagaa
      901 gaaattattt cttttatgaa aaatccaaaa catactggaa ttggttttgg cttaacaaca
      961 ggttattctt ttgctatgaa taaaaaatca ttatggagtg tatcggcaaa ttatcattat
     1021 gccatgttaa ataatgtaaa ttttagtaat gcaggtataa gattacacta ctctcttcca
     1081 ttagttataa tgaataaaaa ataagttttg aagtgtattt ctactcgctt ttttctaaat
     1141 tttagttact tgtatttcat tataatcttg ctttattaat taaataatat tagcttgtta
     1201 aatagtttat taaatgtgaa taaactccga tgattttgcc caaaaaacat ccatataatt
     1261 aaggtattaa gcctataaaa ggttatcttc gccaacgcaa aaattaaatg aaaatctgct
     1321 ttaattagaa tggaagaaaa tacaacacaa ttccccgacc aaaccaatga taacgatcgt
     1381 gtagtgcagg taaatataga agagcaaatg aaaactgctt acatagatta ttctatgagt
     1441 gtaattgttg gtagagcttt gccagatgtg agagatggtt ttaaaccagt tcatcgcaga
     1501 gtattatatg ccatgaacga attaagtaac aattcaggaa aaccatataa aaaaagtgcc
     1561 agaattgtag gagaagtaat gggtaaatat caccctcatg gagatagtgc tatttatgat
     1621 accttagtta gaatggctca ggattttacc atgaggcata ccatggttga tgggcaaggt
     1681 aattttggta cacaagatgg agacccacca gcagcgatgc gttatactga agtaagattg
     1741 caaaaatttg cagaagcaat gttagaagac attgaaaaag acacagtaga ttttcagtca
     1801 aactttgatg atagtttaga agagccaact gttttaccaa ccagaattcc tcagctttta
     1861 gtaaatggta gcagtggtat tgcagtaggt atggcaacta atatgatgcc tcataaccta
     1921 agcgaagtaa tagatggctg tatagcatac atacacaata aagatattac aattgatgat
     1981 ttattgcatt ttgttaaagc acccgacttt ccaacaggtg gcattattta tggaatggaa
     2041 ggcgtgaagc aagggctatt aactggaaga ggtagagttg tgctaagagg taaatgccat
     2101 gttgaaacaa aacaaagtgg cagagaacaa attattatta cagaagtgcc ataccaagtt
     2161 ggattagatg cattaacaga taaaatagga cagctagtaa atgataaaac aattgaagga
     2221 attgcccatg taaataatga aagcaataaa agagaaggta ttagaattgt tatagattta
     2281 aaaagagatg cagtagccaa tattataata aatcaacttt acaagtttac cgaattacaa
     2341 acaagttttg gtattaacaa tgttgcaatc agcaaaggcc gaccaagaat ttttaatgta
     2401 aaagatttaa ttgtagagtt tgttgacttt agaatggatg tggttattcg ccgttctaag
     2461 tttgaactta aaaaggcaga agatagagca catttactag aaggatattt aaaagtaatt
     2521 aatgataaag atcatttaga cttggctata tccataatta gaggcagtgc aacaccagat
     2581 gaagcaaaaa aatcattaat agaaagaagt atagcagata attggtattt acccgataac
     2641 ttatttgatg aaaaagctaa aattcattat ttacagggta ttggtttatc cgaaaaacaa
     2701 gctcaggcaa ttttagaatt acgattacaa agattaacag gtttagaaag ggagaaaatt
     2761 atagaagagt ttaacgacct aatgaatacc attgtgagat taaaagattt attagcaagt
     2821 gaagacttaa gatatgattt aataaaaaca gaattagaag aagtaaaagc aaagtttggc
     2881 gagccaagaa aaagcgaaat tcaatttttg gcagatgaag taaacatgtt agattttatt
     2941 aaagaagaag atgttgtaat tactgtttct catttgggtt atattaaaag aacttccgca
     3001 accgaatatc gtcaacaaaa aagaggtgga agaggagcaa taggaagtaa aacaagagaa
     3061 gaggattttg tagaacactt atttgttgca agcacacacg atgtgatgat gttttttaca
     3121 gaaaaaggcc gttgttattg gttaaatgtt tacgaaatac ccgaaggaga aaaacaaagt
     3181 aaaggaagag caattcaaaa tttaatacaa ataccaccag acgataaaat taaagccata
     3241 atagatgtta aagatttaga caaaaaagaa actttacaaa atagatatgt agtattatgt
     3301 actaaaaaag gtattattaa aaaaacagac cttgaagatt ttagcaggcc acgtaccaca
     3361 ggtattaatg caattaccat cttggaaggc gatgaattat tagaagctaa actaacagat
     3421 ggaaattgcg aaattatgat ggctgttaaa agtggaagag ctatacgatt taatgaagaa
     3481 aaggtaagag caactggtag aggagcaatt ggtgttacag gaattgaagt agatgaaacc
     3541 aatgatgaag tagttggtat gatttgtgtt aataaaaacg atacagacaa aacagtttta
     3601 gtaattagcg aaaaaggata tggtaaaaga acaaaactaa ctgatgaaga tggggaagat
     3661 gtatatagaa taactaacag aggaggcaaa ggggtaaaaa ctattaatgt aacagaaaaa
     3721 accggaagat tagttggcat tttagatgta aatgaaaaag aagatttaat tataacttgt
     3781 caaagtggta ttaccttgcg aacttccatt tcaacaataa aaacggcagg aagaaataca
     3841 caaggtgtaa ttttaataag attagatgca gaggatgcca ttgcagctat ttctaaaata
     3901 gaagaaacag aagaagaaac aactacagaa gtaaatgatc aatcagaaaa taattcacca
     3961 gaaaataccg ataacttaaa cactaatatt gaaaacagta attaaaaaaa taactatgaa
     4021 aaaaataata ttacttgttg catgttccat ttcattggta attgccaatg cacaagacaa
     4081 aatgtataaa aaacttcaaa ccttatacat tgcagctcaa tacgaagaag caaaagctga
     4141 aattgaaaaa gtaatggcca atccaaaagc aatagataaa gcagaaacat ggttgtggcg
     4201 tacaagaatt tatgccgaaa tttacttttc agaaccgtta agaaaattaa acccaggctg
     4261 tggaaatatt gcctttgaat cttttaaaaa atatgaagcc ttagaaagta attataaaat
     4321 gttggctaac ccagaattgt cagcttggag atggagacct ttagatttaa tttatgttac
     4381 tagttttaat ttaggccgta gtttttttga agaaaaaaaa tgggattctt cttacaacac
     4441 ctttatgaaa tcagtttaca tgggtgatgt aattgtgaaa aataatttga gagagaatgg
     4501 agcaaaaatt gatacagtat ctgtattata cgcagcttat gcagcacaaa atggaaaaat
     4561 ggaagcagaa gctacagccc tatacgaaaa atttgctgat tgcaaaatag gaggaaagga
     4621 ttttaaagat gcctatatgt acattttatt atacgcatct aaaacaaaag ataaagccaa
     4681 attcaataaa tatattgcca ttgcaaaaga ggtttatcca aatgatgatg cttgggatga
     4741 ttatgaaatg gaatttatta ccaacaccac tacagttgaa gaaagaattg cctattatga
     4801 taaagaagat gcagctggta ctttaactgc aagaaaatat atgctttttg gccaatcttt
     4861 tagcgaagct acaaaaggag aagaagcaga aaaattagac agtttaacaa aagttaggta
     4921 cgataaaaaa gcagtagaag cttttaaaaa agcatatcaa aaagataata aattaggcat
     4981 tgctgcatac aacgctgggt tactttatta caatgaattt gttgaatacg atgacagaat
     5041 aagtaacaac agaagggctt tacagcaatt aaactctaac aaaccagttg taaaagatcc
     5101 taaaaagaaa gcagtagcag atgctgaatt taaaaaacaa acagatgcta ttaaaaaatt
     5161 aaattcagat ttagagaaac caattttaga aactttagac agttgcgtag tatggttaga
     5221 aatagcttac aataatttaa aaaacggcaa tatggaagat agaacaactt tgaactgtat
     5281 aaaaaattca gtgaaatggt tggcaaatgc ttttatgtat aaaagagaaa aagtgaaagg
     5341 aaaagataca aaagcttatg atgcttacga tgcaaagtat aatgagtacg ataaattgta
     5401 tgaaaaatat taattaaaat ttttcagttt aaataaaagc ctaaactatt tgtttgggct
     5461 tttttactcc tatattgtac attttatgtt tgcttaaatt tattttatat attgcaatta
     5521 tgcaactagg aacagtttat ttaataccta cagttttatt tgaggatgaa ttacattgca
     5581 tacctgacta tgttgtagat actattaaaa attgcgaaat tttttttact gaaaatgaaa
     5641 aaacaacccg acgctttttt aaaaagcttt ggaaagaaat ggtaatagat aattaccagt
     5701 ggcatacaat tcataaaaca gaacaagcag ttgtaaccac tttcttacaa catttaaaag
     5761 atggaaaaaa tgttggaatt gttagcgaag caggatgtcc tggcattgca gatcctggac
     5821 aaattttagt agaagcagct caacaaatta acgcaattat taaacctttg gttggccctt
     5881 catccatttt acttgcatta atggctagtg gaatgaatgg acaatgtttt aagtttaatg
     5941 gatatttgcc aattgatgca atggagagaa aaaagaaaat taaagcactt gaaatggaaa
     6001 gcttacaaca aaactgtaca caaattttta ttgaaacgcc gtatagaaat aatcaactaa
     6061 taaacgatgt gttaagtatt tgcaataagc aaacaaaatt ttgtattgca gttaatatta
     6121 caagtgctgc agaaaaaatt tctactaaat caatagagtc ttggaaaaaa aatattccag
     6181 atatacataa acaacctgct atatatttgt tgaatgcctt ttaatgagat tggagaatat
     6241 taatttaaaa tacaccatac aaataaaata aaagtgatgc taaaaaaaat attaagtacc
     6301 ttattaatta tagttggatt caaagcattt agtcaaacac caatacaaat gtcttcgcaa
     6361 gtaaataatt cttataccga aacatttgca gatattgata attgggtatt taaaaccaca
     6421 tcagaagatg gaacatttac tgcaggtgtg ggtgcaatgt gttggcgtgg tgtagcaatt
     6481 aatgctacag gaactatacc cagtgcacta aaaattacac atgcatctac tttttttcaa
     6541 ataccaccaa caggtaatgg aggctatagt agtggcgtgt ataaaggcaa tcaaaagtta
     6601 gagatactat ctactggtac aactaataat actagttcta ttgcaataga tttttttatg
     6661 gattttacca atgttaatgc tggaacctta agttttgatt gggcttctat taataattca
     6721 tctggcaaca gaagtagttc tttaaaagtg tatgcaaccg tagatggggt aaattatatt
     6781 gaaataacaa acgctcaggt tttaaatttt acaaacaata ctccaacagc aggtagtgtt
     6841 gtaaatgtgc cattacctac aatctttaat aattctgcaa ctgcaagttt aagattttat
     6901 tatcataatg gcacaggagg ttcatctggc agcaggccaa gattaagtat agataatgta
     6961 catgttactg cagtaccaac aacaccatgc actgcaccaa ctatgcagcc tacaaacttt
     7021 actttaggag cagttactca taattctata aatttttctt ttacaagtgc agtgcctgca
     7081 cctcataatt attttatatt aatgagcact aacagtacct taacagcatt gcctgtaaat
     7141 ggtattactt ataatgttgg cgatgctttg gcagatgcaa cagtaattgg tataactaat
     7201 aataatactt ttactgcatc caatttagat ttatcaacta cctattattt tttcttattc
     7261 tctgtaaata attcttgtac aggtgggccc ttatacctta ctgtaaaccc acttaatgga
     7321 agtgcaacaa caactgcagg tgctttacct tgtgctgcac ctacaacaca gccaaccaat
     7381 ttatcattta cttctataac taccaatagt attaaaggca gttttgtacc tgcagcaaat
     7441 accgatgagt atttaattat tagaagttta tcgccaactt ttacaggtat tttaaataat
     7501 ggtacaactt ataatggtgg taatatttta ggaaatggaa tggtagtaac aagaaccgca
     7561 ggtaatactt ttacagcaaa taatttaacc aatggcacaa cttattattt ttttgttttt
     7621 ggattgaata atcaaaactg taccggtggt gctgcatata attctactaa tccactaact
     7681 aataatactt caactgtatc cttacctcca tgtgttacac ctaccaatca accttcacaa
     7741 ttacaattat ctgcatctaa ccattatgta aatggttact ttgtagcagc tccatctgca
     7801 gatggttatt tgatagtaag aacagctatt gcaactttaa gtgtaacacc tcaaaatggt
     7861 ataaattata atgctggcga tggtttagga aatggaacca ttattcaaaa caatgcatca
     7921 acagcattta tagatattaa tttacaaccc tctacaactt attattatca tgtttttgca
     7981 aaaaatagtg tatgtaatgg aaatataaat tatttaagtg taaaccctct tacaggtaat
     8041 acttcaacaa ctgccattgc agtaaataat tggtattatg gaaacttaca ttctcactct
     8101 tcacattcag atggcaacca agataattta tcattaaccc caattgatga ttatgcttat
     8161 gcaaaaaatt cattgtgtat ggattttcta ggaatatcag atcataacca tgcatctgca
     8221 ggaatgagta ttagtaattg gcagccagga ttaaatgctg caagtgcagc aactacttcc
     8281 aactttttgg ctttgtatgg aatggagtgg ggagttatta gtaatggtgg ccatgttttg
     8341 gtttatggct ctaatcaatt aataggatgg gagaccaata actacaatat atatgtacct
     8401 aaaagtgatt atttaggaac gcctgaaacc actggtacaa caggtttgtt tagagctatt
     8461 aataatttgg gtggtaatac atttgcaaca tttgcacacc cttcattttc tgattataat
     8521 ggtatagcaa atatttcgta tagtgcaaca gccgatagtg cattggttgg tagtgctgtt
     8581 gctagtggta ttgcttatag tactaatact acttattcca atccaccaag ttcatttggt
     8641 tatattgatt tttacaatag aatgttaagt aaagggtatc atattggccc aacaatggat
     8701 catgataacc actacactaa ttttggaaga agtaataaca acagattagt agtagtagca
     8761 ccaagcattt caagcaatga tttttattca gcaatgcgta atagaaattt ttatgcatcg
     8821 gaagattgtg atacaagagt tgtatttaca ttaaacaaca aaaaaatggg aagtatttta
     8881 aaagatactg tagcacctgc aatttctgtt tatgcttacg accctaccaa tcctggtttt
     8941 acaccaacaa taaaattaat gtttggtatt gcaggtagcg gagttaatcc aattcaaatt
     9001 tattcatcta atagttctac attaaattat tcagattatg aattaccaat tggttcaaat
     9061 gcttattatt atgcagatat tcaaatagct ggtaacagaa caattactgc accaatttgg
     9121 tttactaaag aagctttatt gcctattaca ctgttaagtt ttaatgccta tttgaataaa
     9181 gaatttaatg tagtaaattt aaactggcaa acaactaacg agataagtaa taattatttt
     9241 attcttgaaa aatcaaataa tggggttgag ttttttagat tagatagtgt gaaagcaaaa
     9301 aatacttctg gcattaataa ttatgcaatt gtagataagc aaccttttga taacattact
     9361 tattatcgtt taaaacaggt tgatatggat ggcaaatact cttattctaa aattattgct
     9421 gtacaattaa ataatctgtt gaatgaattt acaattcaac caaatccaat ttataaccaa
     9481 gctagcgtaa aaattaaatc ttataaaaaa gaaaatacaa ttttaaaaat tatggatatg
     9541 cagggtaggg ttgtattaca gcaattattg caattaaata ttggaacgca gcaaataaat
     9601 attaactgta atagtttgca aaaaggatta tacaatgcaa cactattttt gaataatcaa
     9661 ataataacca gaaaagtggt gaagctataa tagtaagata tagtagttac ttacttggtt
     9721 ttaaatcaat taatttaagt tgtaatattt tttcaccatt ccattcattt tggtcaatgg
     9781 tataaacaat atccacttgg ttattttgtt ctagaataga aaatttattt gccatattaa
     9841 agccaatacc atttaaaata atattgtttt gttttactga aatttttaaa tgttgttctt
     9901 ttacaatttt agaaaagccc gtatctatta catttctaga gataaaaaca ggccgcatat
     9961 tatttggtcc aaaaggctcc atttgcataa taatattata aaatgttgtg gttatttcat
    10021 caaatgcaag ttctgcatca atgataattt caggtattaa taaatctggc gatattgtgt
    10081 ttgataccac agtttcaaaa gcattagtaa aattttctaa ctgttccggt aaaaggctta
    10141 aacctgcagc agcaaagtgt ccaccataat ttgtaagata gtttttacaa gaataaattg
    10201 catcgtacaa attataacca acaacacttc tagcacttcc tgtaattaaa ttgccacttt
    10261 ttgtaagtac aatagttggt ttgtaataag tttcaatcag cctgctagca acaataccta
    10321 ctacaccttt atgccaggtt tcgttgtaaa caactgttgt ttttttatta attaaagtag
    10381 gattattttt tattaaatta agtgcttgtt ccgtaatact gctatctgct tcctttcttt
    10441 ctttattatc tacatgaagc atctcggcat attcaagagc ttttgctaca ttggtttcta
    10501 taaataattg aacagctttt tttgcatcat ccattcggcc tgcagcatta atacgaggag
    10561 ctacaatgaa aactaaatta tttattgata tacttttttc taatgcagct aaacttatta
    10621 aagccttaat accaatatta ggattttcat ttactttc
//
LOCUS       DAOQPK010000059        10630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_3251_length_10630_cov_4.133806, whole genome shotgun sequence.
ACCESSION   DAOQPK010000059 DAOQPK010000000
VERSION     DAOQPK010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10630
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3251_length_10630_cov_4.133806"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..2287
                     /locus_tag="PLU36_04355"
     CDS             95..2287
                     /locus_tag="PLU36_04355"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMZ46013.1"
                     /translation="MMRKRHDLLSKGYFGKLSIGAGNNERYATNANFNRFNGNQQISF
                     IGQGNNINQQNFSMQDLFGTMNSAGGRGGGGMFGGGMRGGNLGNFLTTNQAGIATTWA
                     AGLNYNDVWSKKTSVSGSYFYNNMKRNNDNESHTETFVTNDSSQFNNNNTFSNYLNQN
                     HRFNFEFDHKFDSVNSLLVRPNFSYQISDNNQQTISTLTKGKLVNLSNVQQVTNSQNT
                     GYNFSTSILLRHKFKKRGRTISLNLTPGLSNNESDGSNISYNDIYTINGIVKDTINQI
                     NSNDRNGRTFSSNLSYTEPITKKSQLELSYNYNYSKNNSNQQTLMYNKQTNSFDIIVP
                     NLTNKFENTNTSNKFNANYRVQLTKEWSYTLGMGVQFAELTSINNTKATKLSNTFTNY
                     FPSVQLHYSKNRTKNLRLVYRGQTNAPSITQLQDVIDNSNQLNIRNGNAGLEQEFQHN
                     FNLFYTNFDVITFKNFFVSINGSFTQNKIGNSVIQNTGTTPTVVDGIVLIPGAQYTKP
                     INLNGAMNIFGFINYGFPIKKPKSNLNFSTTMSYIKDVNLYNQTKNYTHNYIIGERVN
                     YNMNVKEVFDVNFASSSSYTFARYTLNNEQNGDYFTQTFSIEPTYSSKSGWIIGSDFD
                     LTINRGQSVGYNQTIPLWNASIAKTIFKNKSGEIKFSVFDLLNQNKSINRTVEQNYIQ
                     DTRTQVLTRYFMLSFTYNLRKFAGKGQQMPPFMRGMMRNAQPRMMRMH"
     gene            2353..3078
                     /gene="tsaB"
                     /locus_tag="PLU36_04360"
     CDS             2353..3078
                     /gene="tsaB"
                     /locus_tag="PLU36_04360"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HMZ46014.1"
                     /translation="MAYLLYIDSVFDTANIYLANNEQIIFADKSLEQKNHASFIQAAI
                     QKNFQKTQINLQSINGIVVVNGPGSYTGVRVGLATAKGLCYALNIPLVLLNTLDIMAF
                     EMKSNLSKFIPLANSTLPILFCPMIDARRMEVFTALYNHNLNYIIKPCASVLNEFSFE
                     NELKENTIFFSGNGSNKLKEVLKNPNAIFTDLLFQASTLIQLGLNQFNKNNFSDLAYS
                     QPFYTKEFYTTASAISKIKGLDK"
     gene            3142..3471
                     /locus_tag="PLU36_04365"
     CDS             3142..3471
                     /locus_tag="PLU36_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMZ46015.1"
                     /translation="MNQTNIVLSNGELSLQIDPLRVKKSAMVIRALNHKLRQQMIKLI
                     DEVKTITVTDIYVRLRLEQSVASQHLAVLRKVGIVATKRDGKKIFYSVNYHRLQHILN
                     TSNNLLN"
     gene            complement(3511..4110)
                     /gene="yihA"
                     /locus_tag="PLU36_04370"
     CDS             complement(3511..4110)
                     /gene="yihA"
                     /locus_tag="PLU36_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HMZ46016.1"
                     /translation="MQIKTAKYLISSPTFDKCPVADKPEFAFIGRSNVGKSSLINMLV
                     NQKNLAKTSGTPGKTQLINHFIVNDSWYLVDLPGYGYAKRSQKDRNRWEQMIEHYMRK
                     RENLINVFVLIDSRHSPQKNDLDFLDKLGKWQIPFVLVFTKADKEKPLVVKRNEQLFL
                     NKMRETWQFLPQSFITSTEKKEGRLQLLQFIDDCVEEQQ"
     gene            4252..5259
                     /gene="gap"
                     /locus_tag="PLU36_04375"
     CDS             4252..5259
                     /gene="gap"
                     /locus_tag="PLU36_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HMZ46017.1"
                     /translation="MVKVAINGFGRIGRLVFRQIYNMQGIDVVAINDLTSPAVLAHLL
                     KYDSAQGRFNAEVSATEDAIVVNGDTVKIYAQKDPAQIPWGSHGVDVVIESTGFFTDK
                     DKAAAHLTAGAKRVVISAPATGDLKTVVYNVNHSILDGSETIISCASCTTNCLAPMAK
                     ALNDNFGLVAGSMTTIHAYTNDQNTLDAPHPKGDLRRARAAAQNIVPNSTGAAKAIGL
                     VLPELKGKLDGGAQRVPTITGSLTELVSVLNKKVTIEEVNAAMKAASNESFGYTEDEI
                     VSTDIIGINFGSLFDASQTKVITQGDTQIVKTVSWYDNEMSYVSQLVRTVKHFAGLIS
                     K"
     gene            5435..6634
                     /locus_tag="PLU36_04380"
     CDS             5435..6634
                     /locus_tag="PLU36_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HMZ46018.1"
                     /translation="MSKFSNHQFANQKALIRVDFNVPLDKTTLAITDDTRMKAAIPTI
                     QKILNDGGSVILMSHLGRPKEGPEDKYSLKHLVQHLSNLLGGKTVFFANDCIGEQATL
                     TASMLKAGEVLLLENLRFYKEEEKGDTAFAEKLSKLGDVWVNDAFGTAHRAHASTAII
                     AKFYAKENRFFGLLMEAEVKAAEQVLHKAQKPFTAIIGGAKVSDKILIIENLLEKATD
                     IIIGGGMAYTFMKAKGGSIGGSLCEDDRLQTALEILEKAKQKNVSIHLPEDSIIADKF
                     AADANTQLTNSNAIPTGWMGLDIGTNAATKFASVINSSKTILWNGPMGVFEMEKFQAG
                     TKAIAVAVAESTQNGAFSLVGGGDSVAAVNQFGFNDKVSYVSTGGGAMLEYFEGKILP
                     GIAAVNE"
     gene            6627..8252
                     /gene="nadB"
                     /locus_tag="PLU36_04385"
     CDS             6627..8252
                     /gene="nadB"
                     /locus_tag="PLU36_04385"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HMZ46019.1"
                     /translation="MNNKTNQMQTDFLVIGSGIAGLTFALKVANHFPDKKITVITKTN
                     SDETNTKYAQGGIAGVIDETNDSFEKHIEDTLIAGDGLCNKEIVEIVVKEGPARIKEI
                     INYGARFDKNEKGDFALGKEGGHSEFRILHHKDVTGKEMERALLQAVTNTKNINIINH
                     CFVVDIITQHHLGYLVTKSTVDITCYGVYVLNLNTNLIEKIESKITLLATGGCGQAYR
                     ATTNPKIATGDGIAMVYRAKGRIENMEFIQFHPTALYEPGISPSFLITEAVRGDGGIL
                     RNKKGEAFMEKYDSRKDLAPRDIVARAIDNEMKFEGTEHVFLDCTHLPKEKFVNHFPN
                     IYQKCLSIGIDVAKSYIPVAPAAHYSCGGVKTNAFAQTSIKNLYACGECASTGLHGAN
                     RLASNSLLEAMVFAHRAFENIVENASEIFERKNIIIIPDWNTKGTIEPREMILITQSF
                     KELQQIMSDYVGIVRTDVRLARAMKRLDLLFEETEQLYRSTKVSPQLCELRNLITTGY
                     LIVKGADFRKESRGLHYNTDHPCKSDIIQNTIL"
     gene            complement(8260..9216)
                     /locus_tag="PLU36_04390"
     CDS             complement(8260..9216)
                     /locus_tag="PLU36_04390"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HMZ46020.1"
                     /translation="MEHIIFYVVILIFILSFFGLFFIVKQQTAQIVERLGKFHSVRNA
                     GFQLKIPFIDTIAGRLSLKIQQLDVIIETKTKDDVFVKLKVSVQYVVIKDKVFEAFYK
                     LDNPMSQITSYVFDVVRAEVPKLRLDDVFEKKDDIAIAVKGELQEAMNTYGYDIIKTL
                     VTDIDPDVEVKQAMNRINAAERDKVSAQYQGDAQRILIVEKAKAEAESKRLQGQGIAD
                     QRREIAKGLEESVIVLNKVGINSQEASALIVVTQHYDTLHSIGSNNHSNLILLPNSPS
                     AAGEMLNNMVASFSAANIIGKELKKMPNTTTTESNELTTNLE"
     gene            9426..>10630
                     /locus_tag="PLU36_04395"
     CDS             9426..>10630
                     /locus_tag="PLU36_04395"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HMZ46021.1"
                     /translation="MVDVILGLQWGDEGKGKIVDFFAPHYDVIARFQGGPNAGHTLYV
                     DGKKVVLHQIPSGIFHQNCVNLIANGVVLDPVTLKRECETVASFGIDVKKNLFISEKT
                     NLIIPTHRALDKASELQKGEGKIGSTLKGIGPAYMDKTGRNAIRVGDLLSKNFTTQYI
                     KLRLKHQKLLDNFHFIEDITAWEEEFFEAIDFLKTLNIVNGEYFINNKLNEGKKVLAE
                     GAQGTMLDIDFGTFPYVTSSNTISAGVCTGLGVSPQKINDVIGVTKAYCTRVGSGPFP
                     TELHDSVGDELRKIGNEFGATTGRPRRCGWIDLVALKYTCMLNGVTKVVMTKADVLDT
                     FENLQVCTSYTIEGKTTQEIPYQISKVAIDPTYQTFKGWNCDSTKINSATNLPANMVT
                     YIKFINEFIG"
BASE COUNT         3923 a         1636 c         1697 g         3374 t
ORIGIN      
        1 ttgtacattt ataatttaaa taaattagat ccacaattaa atcggtcaaa atgtaattct
       61 tttaaataaa aattctatca attatagtgt ttctatgatg agaaaaaggc atgacctact
      121 ttctaaaggc tattttggaa aacttagtat aggtgcagga aataatgaac ggtatgcaac
      181 taatgcaaat tttaataggt ttaatggcaa tcagcaaatt tcatttattg gtcaaggaaa
      241 taatattaac caacaaaatt tttcaatgca ggatttattt ggcacaatga actctgcagg
      301 aggccgtggt ggtggtggaa tgtttggagg tggaatgaga ggtggtaatt taggaaactt
      361 tttaacaaca aaccaagcag gtattgctac aacatgggca gcaggcttaa actacaacga
      421 tgtttggagc aaaaaaacaa gtgtaagtgg cagttacttc tataacaata tgaaaagaaa
      481 taacgacaac gaaagccata ccgaaacctt tgtaactaac gattcttctc agttcaacaa
      541 caacaacact ttttcaaatt atcttaatca aaaccaccga tttaattttg aatttgacca
      601 taaattcgac tctgtaaatt cattactggt tcgtcctaat ttttcttatc aaattagcga
      661 taataaccaa caaaccattt caactcttac aaagggaaag ttggttaatt taagcaatgt
      721 acaacaagtt acaaatagcc aaaacacagg ttacaacttt agtacaagca ttttactaag
      781 gcataaattt aaaaaacgtg gacgcactat ttctctaaac cttacaccag gtttaagtaa
      841 taacgaatca gacggtagca atatttctta taacgatatt tataccataa atggaatagt
      901 gaaagacacc atcaatcaaa taaacagtaa tgatagaaat ggtagaactt ttagtagtaa
      961 cttatcttat acagagccca ttactaaaaa aagtcaatta gagttaagct ataattacaa
     1021 ttacagtaaa aacaattcca atcaacaaac tttaatgtac aataaacaaa ctaatagttt
     1081 tgatataatt gtacccaacc ttaccaataa gttcgaaaac accaatacct ctaataaatt
     1141 taatgctaat tatcgtgtac aactaactaa agagtggagc tacactttag gcatgggtgt
     1201 acagtttgca gagcttacaa gtataaataa tactaaggca accaagttaa gcaatacatt
     1261 cactaattat tttccatctg tacaattaca ctatagtaaa aacagaacta aaaatttaag
     1321 attggtttat agggggcaaa ccaatgcacc atccataaca caactacaag atgtaattga
     1381 taatagcaac caattaaata taagaaatgg taatgcaggt ttagagcaag agtttcagca
     1441 caatttcaat ttattttata ccaattttga tgtaattact tttaaaaact tctttgtaag
     1501 tattaacgga agttttactc aaaataaaat tggcaatagt gttattcaaa atactggcac
     1561 aacaccaaca gttgtagatg gaatagttct tatacctggt gcacaatata caaagcccat
     1621 taacttaaat ggtgcaatga atatttttgg ttttattaat tatggatttc ctataaaaaa
     1681 acctaaatct aatttaaact tttcaactac tatgtcgtat ataaaagatg taaaccttta
     1741 caaccaaact aaaaattata cgcataatta tattattgga gaaagagtta attataatat
     1801 gaatgtaaaa gaagtttttg atgtaaactt tgccagttct tcctcttaca cttttgccag
     1861 atacacttta aataacgagc aaaatggcga ttattttact caaacatttt ctatagagcc
     1921 tacctattca tcaaaatcag gttggattat tggcagcgat tttgatttaa ctataaatcg
     1981 tggtcaaagt gttggttata accaaaccat ccctctatgg aatgcctcaa ttgcaaaaac
     2041 aatatttaaa aataaaagtg gcgaaataaa atttagtgtg tttgatttac taaatcaaaa
     2101 caaaagtata aatagaactg tagagcaaaa ctatattcaa gataccagaa cgcaagtttt
     2161 aacacgttac tttatgctat catttactta taacttaaga aaatttgctg gaaaagggca
     2221 acaaatgcct ccatttatga gaggtatgat gcgtaatgct caaccaagaa tgatgcgtat
     2281 gcattaatca taaaatcaat tttggtactt gccaataatc tttactattt tgtgccaatt
     2341 taatttatta agttggcata tttattatat atagattccg tttttgatac agcaaatatt
     2401 tatttagcaa ataatgagca aataattttt gccgataaaa gtctcgaaca aaaaaaccat
     2461 gcttcattta ttcaagctgc aattcaaaaa aactttcaga aaacccaaat taatttacaa
     2521 tcaataaatg gcattgtagt tgtaaatgga ccaggaagtt atacaggcgt aagagttggt
     2581 ttagcaacag caaaaggctt gtgttatgca ctaaatattc cattagtttt attaaatact
     2641 ttagatataa tggcatttga aatgaagagt aacttatcta aatttattcc tctagctaac
     2701 tctacattac caatattatt ttgcccaatg attgatgccc gaagaatgga agtatttaca
     2761 gccctgtata atcataattt aaattatatt ataaaacctt gtgcaagtgt attaaacgaa
     2821 tttagttttg aaaatgaact aaaagaaaat actatttttt ttagcggaaa tgggagcaat
     2881 aaattaaaag aagtgttaaa aaatcctaat gcaattttta ctgatttact atttcaagca
     2941 tctacattaa ttcaattggg gcttaatcaa tttaataaaa ataacttttc tgacttagca
     3001 tatagccaac ctttttatac aaaagaattc tatacaactg catccgccat tagtaaaatt
     3061 aaaggtttag ataaataatt tttatttgaa aaaaaaaatt gtataaattt acatcctctt
     3121 taacccctaa ttttaataac aatgaaccaa acaaacattg tattgtctaa tggagagttg
     3181 tcgttacaaa ttgatcccct aagagtaaaa aagtctgcaa tggttattcg ggctctcaat
     3241 cataaacttc gtcagcaaat gattaagtta atagatgaag taaaaactat aaccgttaca
     3301 gatatttatg taaggcttag gctagagcaa tctgtagcat cacagcattt ggctgtttta
     3361 agaaaagttg gaattgtagc aaccaaaaga gatggtaaaa agatttttta ttccgtcaat
     3421 tatcatcgct tacaacacat attaaataca agtaacaact tattaaatta atcaattttt
     3481 aaaagatccg tatttactac ggatttttta ttactgctgt tcctcaacac agtcatcaat
     3541 aaactgtaat aattgtaatc ttccttcttt cttttcagta cttgtaataa aactttgtgg
     3601 taaaaactgc caggtttctc tcattttatt taaaaaaagc tgttcatttc tttttactac
     3661 aagtggtttt tctttatctg ctttagtaaa tactaataca aaaggaattt gccatttacc
     3721 taatttatct aaaaaatcta aatcattttt ttgagggctg tgcctactat ctattaatac
     3781 aaaaacattt atcaagttct ctctctttct catgtagtgt tcaatcattt gctcccatct
     3841 atttctatct ttttgagaac gtttggcata accataacca ggcaaatcaa ccaaatacca
     3901 actatcattt acaataaaat gattaattaa ttgggtttta ccaggtgtac cagatgtttt
     3961 agctaaattt ttctggttta ccaacatatt aattaaagac gattttccaa cattacttct
     4021 accaataaat gcaaattctg gcttatctgc aacagggcat ttatcaaacg taggcgatga
     4081 aataaggtat ttagcagttt taatttgcat aatgtaaaaa taaagttata tagcaaccag
     4141 ccaaatatct aatcttgttt tactaaatag gttagtaaat tgtataattg ccattaaaac
     4201 tttaattttg caccactttt cagtttttta aacattttaa aaatttaact catggtaaaa
     4261 gttgctatta atggttttgg acgaattggt cgtttagtat ttcgtcaaat ttataacatg
     4321 caaggtatag atgttgttgc aatcaacgat ttaacctctc ctgcagtgtt agcacattta
     4381 ttaaaatacg atagtgcaca agggcgtttt aatgcagagg tttctgctac agaagatgcc
     4441 attgttgtta atggagatac agtaaaaata tatgctcaaa aagatcctgc tcaaattcct
     4501 tggggaagcc atggagtaga tgttgtaatt gaatcaacag gcttctttac agataaagat
     4561 aaagcagcag cacacttaac agctggtgca aaacgtgtag taatttctgc tcctgcaaca
     4621 ggcgatttaa aaacagttgt atataatgta aaccatagca ttttagatgg ttcagaaact
     4681 atcattagct gtgcatcttg tactacaaat tgcttagctc caatggctaa agctttaaac
     4741 gataattttg gtttagtggc aggtagtatg accacaattc atgcttacac taacgaccaa
     4801 aacactttag atgctccaca tccaaaaggt gatttacgca gagcaagagc tgcagcacaa
     4861 aatattgtac caaacagtac aggtgcagct aaagctatag gcttagtttt accagaatta
     4921 aaaggtaagt tagatggtgg tgcacaacgt gtacctacaa ttacaggttc attaaccgaa
     4981 ttagtaagtg tgttaaacaa aaaagtaact atagaggaag taaatgcagc aatgaaagca
     5041 gcaagcaacg aaagttttgg ttataccgaa gatgaaattg taagcactga tattataggt
     5101 ataaattttg gtagcttgtt tgatgcatca caaacaaaag ttattacaca aggcgataca
     5161 caaattgtaa aaactgttag ctggtacgat aatgaaatga gctatgttag ccaattagtt
     5221 cgtacagtaa aacattttgc tggtttaata agcaaataat ttttatgtta ccaatacaag
     5281 atatgctatt caacacctgt tgcgtcgcac acttgtactt taataattct attcaaaata
     5341 aaagttccga aaataaaatt tcggaacttt tgtatttata aataagcaat accttcacaa
     5401 aaaatttaac ccttcatttt ctaaaaatat tattatgagc aaattttcaa accaccaatt
     5461 cgcaaaccaa aaagcattaa ttcgtgtaga ttttaatgtg ccattagata aaacaacctt
     5521 agcaattaca gatgatacaa gaatgaaagc tgcaatacct accattcaaa aaatattaaa
     5581 cgatggtggc agtgtaattt taatgagcca tttaggaaga cccaaagaag gaccagaaga
     5641 taaatattca ttaaaacatt tagtacaaca cttaagtaat ttattaggcg gtaaaacagt
     5701 tttttttgca aatgattgta ttggtgaaca agcaacactt acagcatcaa tgcttaaagc
     5761 aggagaagtt ttattgttag aaaatttacg attttataaa gaagaagaaa aaggagatac
     5821 agcgtttgca gaaaaacttt caaaactagg agatgtttgg gtaaacgatg cttttggcac
     5881 tgcacataga gcacatgcat ctactgcaat tattgcaaaa ttctatgcaa aagaaaatcg
     5941 attttttggt ttattaatgg aggcagaagt aaaagcagca gaacaagtac ttcacaaagc
     6001 acaaaaacct ttcacagcta ttattggcgg agccaaagta tcggataaaa tattaattat
     6061 tgaaaacctt ttagaaaaag caacagatat aattattggt ggtggcatgg cttatacatt
     6121 tatgaaagct aaaggaggca gtattggagg ctctctttgc gaagacgaca gattgcaaac
     6181 tgctttagaa attttagaaa aagccaagca aaaaaatgta tctatacatt taccagaaga
     6241 ttcaataatt gcagacaagt ttgctgcaga tgcaaataca caattaacaa acagtaatgc
     6301 aataccaaca ggttggatgg gcttagatat tggcacaaat gcagcaacta aatttgccag
     6361 tgtaattaat agttctaaaa caattttatg gaatgggcca atgggtgttt ttgaaatgga
     6421 aaaatttcag gctggtacaa aagcaatagc tgttgcagta gcagaaagca cacaaaatgg
     6481 agcctttagc ttagttggtg gtggagatag tgtagctgct gtaaaccaat ttggatttaa
     6541 tgataaagtt agttatgtaa gtacaggcgg tggagccatg ttagaatatt ttgaaggtaa
     6601 aattttaccg ggtattgcag cagtgaatga ataacaaaac taaccaaatg caaacagatt
     6661 ttttagtaat tggtagcggt attgctgggt taacttttgc actaaaagtt gccaaccatt
     6721 ttccagataa aaaaattact gtaattacca aaaccaattc agacgaaacc aataccaaat
     6781 atgcccaagg tggtattgca ggtgttatag atgaaaccaa tgatagcttt gaaaaacata
     6841 ttgaagatac tttaattgct ggcgatggtt tatgcaataa agaaattgta gaaatagttg
     6901 taaaagaagg gccagctaga ataaaagaaa ttattaatta tggagcaaga tttgataaaa
     6961 atgaaaaagg agattttgct ttaggaaaag aaggagggca tagcgaattt agaattttgc
     7021 atcataaaga tgtaacaggt aaagaaatgg aacgtgcctt attacaagct gttactaata
     7081 caaaaaatat caacatcatt aatcattgtt ttgttgtgga tattattacg caacatcatt
     7141 taggttattt agttacaaaa tctactgtag atattacatg ttatggtgta tatgttttaa
     7201 acttaaacac caacttaata gaaaaaatag aaagtaaaat aactttatta gcaactggtg
     7261 gttgtgggca agcttataga gcaactacta accccaaaat tgctacaggc gatggcattg
     7321 caatggtata tagagctaaa ggtagaattg agaatatgga gtttattcaa tttcatccaa
     7381 cggcattata tgaacctggt atttctcctt cttttttaat tacagaagcc gttcgtggcg
     7441 atggtggcat tttacgcaac aaaaaaggag aagcctttat ggaaaaatat gatagccgta
     7501 aagaccttgc tccaagagat attgttgcta gggctataga caatgaaatg aaatttgaag
     7561 gcaccgaaca tgtgttttta gattgtactc acctacccaa agaaaaattt gttaaccact
     7621 ttccaaatat ttatcaaaaa tgtttaagca taggtattga tgttgccaag agctatatac
     7681 ctgttgcacc tgcagcacac tatagttgtg gtggcgtaaa aacaaatgca tttgcacaaa
     7741 cttctattaa aaatttatat gcatgtggcg agtgtgcaag cacaggctta catggtgcaa
     7801 atagacttgc tagcaatagc cttttagaag caatggtttt tgcacatagg gcatttgaaa
     7861 atattgttga aaatgcctcc gaaatttttg aaagaaaaaa tattattata ataccagatt
     7921 ggaatacgaa aggtactatt gaacccagag aaatgatatt aatcacccag agttttaaag
     7981 agctacaaca aattatgagt gattatgttg gtattgtaag aacagatgta cgtttagcac
     8041 gtgctatgaa acgtttagac ctattatttg aagaaacaga acaattatat cgttccacaa
     8101 aagtatctcc acaactttgt gaactaagaa acctaataac tacaggttat ttaattgtaa
     8161 aaggtgccga ttttagaaag gaaagcagag gattacatta taataccgac catccttgta
     8221 aaagcgatat aatacaaaac actattttat aaaataacac tattctaaat tagttgttaa
     8281 ttcattactc tctgtggtag tagtattagg cattttcttt aattcttttc ctataatatt
     8341 agcagcacta aaacttgcta ccatgttatt aagcatctcg ccagcagcac ttggcgaatt
     8401 aggaagtaaa attaaattag aatgattatt agaacctatt gagtgtaatg tatcataatg
     8461 ttgtgtaacc acaattaaag cagaagcttc ttgcgaattg ataccaactt tgttaagaac
     8521 aataaccgat tcttctaggc cttttgcaat ttcccttctt tggtctgcaa taccttgccc
     8581 ttgtagtctc ttactttcag cttcagcctt ggctttttca acaattaaaa ttctttgagc
     8641 atcaccttga tattgggcag aaaccttgtc tctttctgct gcattaattc tgttcatagc
     8701 ttgctttact tcaacatcag ggtcaatatc ggtaaccaat gtttttatta tgtcgtaccc
     8761 ataagtattc atagcttctt gcaattcgcc tttaactgca attgctatat catctttttt
     8821 ctcaaaaaca tcatctaatc tcaacttagg aacctcagcc ctaaccacat caaatacata
     8881 agaagttatt tgcgacattg gattatctaa cttataaaaa gcttcaaaca ccttatcttt
     8941 aattacaaca tattgcaccg acactttcaa ttttacaaat acatcatctt ttgtttttgt
     9001 ttctataata acatctaact gttgaatttt taaactcaat cttcctgcaa tagtatcaat
     9061 aaatggaatt tttaactgaa agcctgcatt acgcacactg tgaaattttc ctaaccgttc
     9121 aactatttgt gcagtttgtt gctttacaat aaaaaacaac ccaaaaaaag ataaaataaa
     9181 aattaagatt actacataaa aaataatgtg ttccatatat tataagtttt atttttttaa
     9241 tgatagttaa agtgccaata tacaaaaatt aagctaataa aaaagtaaaa ttaattattg
     9301 agtttttagt tgcctactca aaaaaaataa aaattcttaa caaaaaatgt caaatactcc
     9361 acaatacaca atttcaattt ttttgcagca atgcattaca tacatttgcc catataattt
     9421 attttatggt tgatgttatt ttaggcttgc aatggggaga tgaaggcaag gggaaaattg
     9481 ttgatttttt tgctccacat tacgatgtta tagcacgttt tcaaggtggt ccaaatgctg
     9541 gtcatacttt atatgtagat ggtaaaaaag ttgtattaca tcaaatacct tctggcattt
     9601 ttcatcaaaa ctgtgttaac ctaattgcaa atggtgtagt acttgatcct gtcactttaa
     9661 aacgtgaatg tgagacagtt gcttcatttg gcattgatgt aaagaaaaat ttatttattt
     9721 cagaaaaaac aaatctaatt ataccaacgc atagagcctt agataaagca agtgaactac
     9781 aaaaaggaga aggaaaaatt ggtagcactt taaaaggtat tggccctgca tatatggata
     9841 aaacaggacg taatgcaata cgtgtaggtg atttattaag taaaaatttt acaacacaat
     9901 acataaaact tcgtttaaag caccaaaaat tattagataa ttttcatttt attgaagaca
     9961 ttactgcatg ggaagaggaa ttttttgaag ccatagattt tttaaaaact ttaaatattg
    10021 ttaacggaga atattttata aacaacaaat taaacgaagg aaaaaaagtt ttagcagaag
    10081 gggcacaagg tactatgtta gatatagatt ttggcacttt cccttatgtt acatcttcca
    10141 acacaattag tgcaggcgta tgtacaggct taggtgttag ccctcaaaaa attaatgatg
    10201 taattggtgt tactaaagca tattgtacaa gagtaggcag tggccctttt ccaactgaat
    10261 tgcatgatag tgttggcgat gaacttagaa aaattggtaa tgaatttgga gctacaacag
    10321 gtaggcctag gcgttgtggt tggatagatt tagtagcttt aaaatatacc tgtatgttaa
    10381 atggtgtaac caaagtagta atgactaaag ccgatgtgtt agatactttt gaaaatttac
    10441 aagtatgcac cagttacaca attgaaggaa aaaccactca agaaattcct taccaaatta
    10501 gcaaagtagc cattgaccct acctaccaaa cttttaaagg atggaactgc gattctacaa
    10561 aaattaattc tgcaaccaac ttaccagcta acatggtaac ttatattaaa tttattaatg
    10621 aatttattgg
//
LOCUS       DAOQPK010000060        10566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_3280_length_10566_cov_4.409000, whole genome shotgun sequence.
ACCESSION   DAOQPK010000060 DAOQPK010000000
VERSION     DAOQPK010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10566
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3280_length_10566_cov_4.409000"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..1057
                     /locus_tag="PLU36_04400"
     CDS             245..1057
                     /locus_tag="PLU36_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HMZ46022.1"
                     /translation="MNTIQAIVIAIIEGLTEFLPISSTAHMGFTAHLLGLNNNDEYLK
                     MYQVSIQFGAILAVVVAYWSKFFQFKNIKFYSKLIIAVIPALVLGKLFDDKIEMVLGN
                     QIAISVVLVLGGIVLLFVDNWFKQPKITDEEQISYKKSLTIGFFQCLAMMPGTSRSAA
                     SIIGGMALGLSRKVAAEFSFFLAVPTMLAVTLYSVFIKTWGEGTVQQAKGYTMITANS
                     NNFTNFIIGNIVAFIVALLAIKFFIALLNKFGFKMWGWYRIIIGIVLLFFFI"
     gene            1143..1613
                     /locus_tag="PLU36_04405"
     CDS             1143..1613
                     /locus_tag="PLU36_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46023.1"
                     /translation="MKKNIFYVLFMITFISACNFFGSSYGKRIEISDKVEVYIKGDST
                     TEEDGKKLGTYLDTTWKDLTNKRSFQLIKDKGEYNVSMVVDETKLKEDSTLEESFKLL
                     RLLIAMSVFPENKVNLILTNDTFKPYKTYKDEGKNTMIEEDAIEPVATDTTTSK"
     gene            1705..3012
                     /locus_tag="PLU36_04410"
     CDS             1705..3012
                     /locus_tag="PLU36_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ46024.1"
                     /translation="MTNSPKKIINAWAMYDWANSAYNLVITSTMFPAYFEAVTKSNNN
                     EVRFLGRTFVNTSLYNYALGFAFLIVAFISPILSSIADYKGNKKSFLRFFCTIGSIAC
                     SGLYFFDKTNVGLGILLMITACVGYWASLVFYNSYLPEIAEEKDRDWVSAKGFSLGYI
                     GSVLLQIICFVLLFKKEWFGISNDSRALQISFLLVGIWWFGFAQKTLNVLPKGIASEN
                     RPEKNIFINGFIELKKVWKILKEMPLLKRFLFAFFFYNMGVQTVMLAATLYGKSELAI
                     PINNLIIAILIIQLVAIPGAILLSKLSAIIGNLQTLVLAVIFWICICIAGYYLPKGNI
                     NLFYVLAVGVGFIMGGIQSLSRSTYAKLMPQLKETTSFFSFFDVTEKIAIVLGMFGYG
                     FVTELAGGSQRNSVLLLAILFIIGLIILLFTIQFQKKQQYATL"
     gene            2999..3868
                     /locus_tag="PLU36_04415"
     CDS             2999..3868
                     /locus_tag="PLU36_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ46025.1"
                     /translation="MQLYNIETENFKLDGGAMFGVVPKSIWNKLNPADENNMCSWAMR
                     CLLIQDGNRLILVDNGIGNKQDAKFFSYYFLHGENTLEKSLAKYGFHKNDITDVILTH
                     LHFDHCGGSVDRIDNKLIPAFKNATYWSNQNHWQWATQPNDREKASFLKENILPIQEN
                     GQLQFINLPNTTLEQTGKLGMSSFNEFIDLRFVNGHTDMMMLPQIKYKNKTIVYMADL
                     LPSAAHIPLPYVMAYDMFPLTTLKEKKSFLQEAVTNEYILMFEHDVHNECCTLQQTER
                     GIKVNEIFKLSEI"
     gene            complement(3873..5048)
                     /locus_tag="PLU36_04420"
     CDS             complement(3873..5048)
                     /locus_tag="PLU36_04420"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HMZ46026.1"
                     /translation="MQEAYIVAGYRTAVTKSKKGGFRFYRPDDLAVEVIKALKATIPQ
                     LESNRIDDVIVGNAVPEAEQGLQFGRIIAAKALGIEVPGITINRYCASGLESIAMATA
                     KIRTGMAECIIAGGTESMSLVPTAGWKTVPSYAIAKDEPDYYLSMGLTAEAVAKEYNV
                     NREQQDEFAYHSHRKAAIAINEGYFKSGILPINVEEVYLNEKGKKASRNYVVDTDEGL
                     RADTTIEGLAKLKPAFSQGGTVTAGNSSQTSDGAAFVIVMSEKMVKELNVQPIGRLVN
                     CASAGVHPRIMGVGPIAAIPKVLKQANMQLNNIDLIELNEAFASQSIAVINNLGLNND
                     IVNINGGAIALGHPLGCTGAKLTIQILNDMKRLNKKFGLVTACVGGGQGIAGIIENF"
     gene            complement(5168..6043)
                     /locus_tag="PLU36_04425"
     CDS             complement(5168..6043)
                     /locus_tag="PLU36_04425"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HMZ46027.1"
                     /translation="MSAIGKSSLKRSRPSYIYSIVGVALVLLMMGIIGWMFLNSKAMT
                     KVFKEDVKISVYLRTMNKDTISQIQQFISNQPYAKDVHYIDKEQAKKIWNAENNEDWA
                     QFLDVNPLPESIDFFAKADYVNKDSLEVIQKSINTAYQYQITEVNYPPVLVNNLDAKA
                     RQIGLILLVVAIILAVVVIISIDNTIKLAMFSNRFLIKTMQMVGATRSFIARPMNIRA
                     VVNGLISGAIAVVLLLTIILWAESYFPELKKIRDLQLTLTLFGGMILLGIAISVYSTH
                     RSILKYLKMKLDDLY"
     gene            6213..9029
                     /gene="leuS"
                     /locus_tag="PLU36_04430"
     CDS             6213..9029
                     /gene="leuS"
                     /locus_tag="PLU36_04430"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HMZ46028.1"
                     /translation="MDYKFREIEEKWQNYWIENKTYKVYNNSSKPKFYVLDMFPYPSG
                     AGLHVGHPLGYIASDIYSRYKRLKGFNVLHPMGFDSFGLPAEQYAIETGMHPAETTKK
                     NIATFKKQLNKIGFCYDWDREVQTSSPAYYKWTQEIFLKLYNSYFCNTQQKARPITDL
                     IEKYATKGTKHFSSNQWNAFTDAEKEVWLMKRRLAFSSYGEVNWCEALGTVLANDEVV
                     NGVSERGGHPVIKKKMRQWYLRITAYADRLLQGLETVDFSDSMKEMQRNWIGKSEGAE
                     INFKISKPFKDDTNNNKEFSDVIKVYTTRPDTIFGVDFMVIAPEHEMVETIISEEQKL
                     YVEKYIAYVKSRSDRERQAEKNISGVFTGSYAINPFDGKKIPIWISEYVLIGYGTGAI
                     MAVPCGDERDFKFAKHFNIPITNIIGNHFNGLEANPTKEATLENSNFLNGLVMKDAIT
                     IAINKLEELGIGKRKVNYKMRDAGFSRQRYWGEPFPIIWKEGTPTALSASQLPLELPH
                     VEKYSPGPEGEGPLANIQNWINTPEGKRESSTMPGYAGSSWYFLRYMDPENNQEFVSR
                     TASDYWNSVDLYIGGTEHAVGHLLYSRMWTKVLYDLGYIGFDEPFKRLVNQGMIQGSS
                     RFVYRINGTNTYVSKNLISEGDQYLYQEKKVDKMHVDVNMVDGLELNIEQFKQWRNGE
                     NNNAEFILEGEKYICGVEVEKMSKSKYNTVNPDDLCVQYGADTFRMYEMFLGPVEQSK
                     PWDTKGIEGVHRFLKKLWRLFYSESSFSNGIQTNGNTIFTKDAATSEELKVLHKTIKK
                     IDEDTERFSYNTAVSAFMVCVNELADLKCFKKEILENVLILLAPYAPHIAEELWNVLG
                     NEGSVLDAAYPIYNSNYTIESSKEYPVSINGKLRTTMHFDINAEQKDVEPVVLANEIV
                     QKWLEGKNPKKIIFVKNKMINIVV"
     gene            9055..9885
                     /locus_tag="PLU36_04435"
     CDS             9055..9885
                     /locus_tag="PLU36_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HMZ46029.1"
                     /translation="MFENVITYFSTLEQRPLERLAFLVVGLLFFWIMEGYIPLITMQY
                     KKNKLKHAGVNFAFTVIHLIIHTFLALAIIKISDWCLANNFGLVYWIKTNVLFTILIS
                     FLILDFFGGWLVHIVEHKIPLLWRFHVIHHSDNNVDVTTGLRHHPIESVIRGIFFFAG
                     IVVSGAPMYAVMIFQTILVISTAFTHANIALPLWLDKLLSYFIVSPNMHKVHHHYKQP
                     FTDSNYGAILSIWDRLLGTFSHLHHSKIKYGLDNFYPNEKDEDFMLLMKKPFGKMTKE
                     "
     gene            9917..10507
                     /locus_tag="PLU36_04440"
     CDS             9917..10507
                     /locus_tag="PLU36_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase hexapeptide repeat family protein"
                     /protein_id="HMZ46030.1"
                     /translation="MIYQFKNYIPVVHQSSFVHPQAAVTGNVIIGKNVYVAPGAAIRG
                     DWGKIIIEDGCNIQENCTIHMFPGITVVLKEAAHIGHGAIIHGATIGKNCLIGMNAVI
                     MDNVVLGNECIVGALSFIKADEKFEDRSLIVGNPAKKIKEVSNEMIAWKTEGTKLYQQ
                     LPTELHAYLKAVEPLREMPSNSNNEESVYKTWNETK"
BASE COUNT         3663 a         1521 c         1761 g         3621 t
ORIGIN      
        1 ctactgcatt ttctaacaat tcttttacgg cacttgctgg tcgttgaata acttctcctg
       61 ctgcaatttg gttagcaata ctatctggta aaagttcaat aatatctgtc actacaattt
      121 tattaaatgg gttcaaaagt agttttttat ttatgttctt ttataaactt tattttaaaa
      181 tacaataaac attttgaata gttctcaaac actgttttct ttgtttatta ctaaaagcta
      241 ttaaatgaat accatacagg ccattgttat tgcaataata gaagggctaa ccgagttttt
      301 gcctatttca tctacagctc acatgggttt tactgcacat ttattaggtt taaataataa
      361 cgatgaatat ttgaaaatgt accaggtgag tattcagttt ggagcaattt tagcagttgt
      421 agtagcttat tggagtaaat tttttcagtt taaaaatatt aaattttata gtaaactaat
      481 tattgctgta ataccagctt tggttttagg aaaattattt gatgataaga ttgaaatggt
      541 tttaggcaat caaattgcaa taagtgttgt tttagtttta ggaggtattg tattattatt
      601 tgttgataat tggtttaagc aaccaaaaat tacagatgaa gagcaaatta gctataaaaa
      661 aagtttaact attggctttt ttcaatgctt ggctatgatg cctggtacta gccgtagtgc
      721 agcttctatt attggtggca tggcattggg gttaagtaga aaagttgctg cagagttttc
      781 ttttttctta gcagtaccaa ccatgttggc agtaacattg tattctgttt ttataaaaac
      841 ctggggagaa ggtacagtgc aacaagccaa gggttataca atgattacag ccaacagcaa
      901 taattttact aattttatta taggcaatat agttgctttt attgttgctc tgcttgcaat
      961 taaatttttt attgcattat taaataagtt tggttttaaa atgtggggtt ggtacagaat
     1021 tataatagga attgttttac ttttcttttt tatatagttt ttaaaattaa aatacccaat
     1081 aacacttatc ttagatacca aaaattaatt ctttctttgt ttcatcctct aaaaaaaaaa
     1141 ttatgaaaaa aaatattttt tatgtgctat ttatgataac atttattagt gcatgtaact
     1201 ttttcggatc ctcttatggt aaaagaattg aaattagtga taaagttgaa gtatatatta
     1261 aaggagatag tacaactgaa gaagatggta aaaagttagg tacttattta gacacaacat
     1321 ggaaagacct taccaacaaa agatcttttc aattaataaa agataaagga gaatacaacg
     1381 taagtatggt ggttgatgaa acaaaattaa aagaagattc taccttagag gagagtttta
     1441 aactattacg tttattaatt gcaatgagtg tttttccaga aaataaagta aaccttatac
     1501 ttaccaacga tacttttaaa ccttacaaaa cttataaaga cgaaggtaaa aatacaatga
     1561 tagaagaaga tgctattgag ccagtagcaa cagataccac tacaagtaaa taaccatttt
     1621 attttaaata aaattattag gcattgtaaa aaaatacaat gctttttttt gtttacttat
     1681 ctttaaattt caaaaaaagc ctacatgaca aattcaccta aaaaaattat taatgcatgg
     1741 gcaatgtacg attgggcaaa tagtgcatat aatttagtga ttacttccac tatgtttcca
     1801 gcatattttg aggctgtaac aaaaagcaat aataatgaag tacgattttt aggtagaaca
     1861 tttgtaaata cttctttata taattatgca ttaggatttg catttttaat tgtagcattt
     1921 atttcgccta ttttatcttc tattgctgat tataaaggca ataaaaaatc ttttcttcgt
     1981 tttttttgta ctattggcag tatagcttgt agcggtttat acttttttga taaaaccaat
     2041 gttggcttag gtattctatt aatgattaca gcatgtgttg gttattgggc tagtttagta
     2101 ttttacaatt catatttacc agagattgca gaagaaaaag acagagactg ggttagtgca
     2161 aaaggctttt ctttaggcta tataggaagt gtattattac aaataatttg ttttgtttta
     2221 ttatttaaaa aagaatggtt tggtatatct aatgatagca gagcattaca aatttctttt
     2281 ttattagtag gcatttggtg gtttggcttt gcacaaaaaa cattaaatgt tttacccaaa
     2341 ggcatcgcct ctgaaaatag gccagaaaaa aatattttca tcaatgggtt tattgaatta
     2401 aaaaaagtat ggaaaatttt aaaagaaatg cctttgctta agaggttttt atttgcattc
     2461 tttttttata atatgggggt acaaactgtt atgcttgctg ctactttata tggcaaaagc
     2521 gaattagcca tacctataaa taatttaatt attgccatat taattattca gttagtggcc
     2581 atacctggag caattctttt atccaagtta tctgcaataa ttggtaattt acaaacgctt
     2641 gttttggctg taatattttg gatttgtatt tgcattgctg gttattattt acccaaaggc
     2701 aatattaatt tgttttatgt tttggcagta ggtgttggat ttattatggg tggaatacaa
     2761 agtttaagta gatctaccta tgctaaacta atgccacaat taaaagaaac aacttcattt
     2821 tttagttttt ttgatgtaac cgaaaaaatt gctattgttt taggtatgtt tggttatggc
     2881 tttgttacag aattggcagg tggttctcaa agaaactctg ttttattatt ggctattttg
     2941 tttataatag gattaataat attattgttt acaattcaat ttcaaaaaaa acagcaatat
     3001 gcaactttat aatatagaaa cagaaaattt taaattagat ggaggtgcca tgtttggggt
     3061 tgtgccaaaa agtatttgga ataaattaaa ccctgcagat gaaaacaata tgtgtagctg
     3121 ggctatgcgt tgtttattaa ttcaagatgg caatagatta attttagtag ataatggaat
     3181 tggaaataaa caagatgcca aatttttttc gtattatttt ttacatggag aaaatacttt
     3241 agaaaaatct ttagcaaaat atggctttca taaaaatgat attaccgatg taattttaac
     3301 tcatttacat tttgaccatt gtggaggtag tgtagataga atagataaca aactcatacc
     3361 tgcttttaaa aatgcaactt attggagtaa tcaaaaccac tggcaatggg ctacacagcc
     3421 aaacgataga gaaaaagctt cttttttaaa agaaaatatt ttaccaattc aagaaaatgg
     3481 gcaactacaa tttattaatt tgcccaatac tactctagag caaacaggaa aattaggaat
     3541 gagtagtttt aatgaattta ttgatttaag atttgtaaat ggccataccg atatgatgat
     3601 gttgccacaa ataaaataca aaaacaaaac tattgtttac atggcagatt tgctacctag
     3661 tgcagctcat attccactac cctatgtaat ggcatacgat atgtttccgt taacaacctt
     3721 aaaagaaaaa aaatcgtttt tacaagaggc tgttacaaac gaatatattt taatgtttga
     3781 gcatgatgta cacaacgaat gctgtacctt acaacaaaca gaaagaggaa taaaagtgaa
     3841 tgaaatattt aaacttagcg aaatataacc ttttaaaaat tttcaataat tccggcaatc
     3901 ccttgccctc ctccaacaca agcagtaact aagccaaatt ttttgtttag tcgcttcatg
     3961 tcatttaaaa tttgaatagt gagttttgct cctgtgcaac ctagtggatg ccctaatgca
     4021 atagcacctc cattaatatt tacaatatca ttatttaagc ccaaattatt aataacagca
     4081 atgctttgcg acgcaaatgc ttcgtttaat tctattaaat caatattatt caattgcata
     4141 ttggcttgtt ttaaaacctt tggtattgca gcaattggcc ccacacccat aatacgaggg
     4201 tgtacacctg cacttgcaca atttactaac ctgccaatag gttgtacatt tagctctttc
     4261 accattttct cactcataac aattacaaaa gcagcaccat cactagtttg agaactgttg
     4321 cctgcagtaa cagtgccccc ttgagagaaa gcaggtttta gttttgccaa accttctata
     4381 gtggtatctg ctcttaagcc ttcatcggta tcaacaacat aatttctcga agccttttta
     4441 cctttctcgt tcaaataaac ttcctccaca tttattggta aaatacccga tttaaaatag
     4501 ccttcattta ttgctatagc agcttttctg tgagaatggt aagcaaactc atcttgttgt
     4561 tctctattaa cattatattc ttttgcaact gcttcggcag ttaaacccat gcttaaataa
     4621 taatcaggtt catcttttgc aatagcataa gagggtactg ttttccagcc tgctgttggt
     4681 acaaggctca tactttctgt gccaccagct ataatacact ctgccatacc tgttctaatt
     4741 tttgcagtag ccattgcaat actttctaat ccacttgcac aatatctatt aattgtaata
     4801 cctggcactt caatacctaa agcttttgct gctattattc ttccaaactg tagcccttgt
     4861 tctgcttcgg gtactgcatt tcctacaatt acatcatcta ttctatttga ctctaactga
     4921 ggtatagtag cttttaaagc ttttataact tctacagcta aatcatcagg tcggtaaaaa
     4981 cgaaaacctc ctttcttact ttttgtaaca gcagttctat agcctgcaac tatatatgct
     5041 tcttgcataa aatacaattt taaaatccaa atgtaaagaa agttgtttat gcaaccaatt
     5101 taatttttgt tacaaaacaa tatggtacat aaagtggaaa aatattgata atattagcaa
     5161 gattgaatta atataaatcg tctagcttca tttttaagta ttttaaaata ctacgatggg
     5221 tgctataaac actaattgca atacctaata aaatcattcc accaaaaaga gttaaggtaa
     5281 gctgtaagtc tctaattttt ttcaattcag gaaagtaaga ttctgcccaa agaataattg
     5341 ttagcaataa aactactgct attgcaccac taattaatcc attaactact gctctaatat
     5401 tcattggcct ggcaataaaa cttctggtgg cacctaccat ttgcatggtt ttaattaaaa
     5461 atctattgct aaacatggca agttttattg tattatcaat actaataatt actacaacag
     5521 ctaatataat tgccacaacc aataatatta aaccaatttg ccttgctttg gcatctaaat
     5581 tatttactaa tactggcgga tagttaactt ctgtaatttg atattgatag gctgtattaa
     5641 tgcttttttg aattacctct aaactatctt tatttacata atctgcctta gcaaaaaaat
     5701 ctatactttc tggaagtggg tttacatcta aaaattgagc ccagtcttca ttattttctg
     5761 cattccatat ttttttagct tgttctttat caatataatg aacatcttta gcatagggtt
     5821 gattacttat aaactgttga atttggctta tggtatcttt attcattgtt cttagataca
     5881 cacttatttt tacatcttct ttaaaaacct tcgtcattgc tttactattc aaaaacatcc
     5941 atccaataat gcccatcatt aacaacacca atgctacccc aacaatacta tatatataac
     6001 ttgggcgact acgttttaaa gaagattttc ctattgctga catgtattta atttaaagtg
     6061 tacaactgct acaaatgtag gtaaacactt acatttgcct ttgcttaacg aacaagtgtg
     6121 cataaagatt ataaatgatt taggttttta aggttaaaaa tttgttacac taattttttt
     6181 ttacattttt aaacaaatat ttaaagggaa ttatggatta taagtttaga gaaattgaag
     6241 aaaaatggca aaattattgg atagaaaata agacttataa ggtttataac aacagtagta
     6301 aacccaaatt ttatgtgtta gatatgtttc cttacccaag tggggcaggt ttacatgttg
     6361 ggcacccatt aggttatatt gccagcgata tttactccag atacaaacgc ttaaaaggct
     6421 ttaatgtttt acaccctatg ggttttgata gctttggctt acctgcagaa cagtatgcaa
     6481 ttgaaacagg tatgcaccct gccgaaacca ctaaaaaaaa tattgccact tttaaaaagc
     6541 aattaaataa aataggcttt tgttacgatt gggacagaga agtgcaaacc agtagcccag
     6601 cttattacaa atggacacag gaaatatttc taaaacttta taacagctat ttctgcaaca
     6661 ctcagcaaaa agcaaggcca attaccgatt tgattgaaaa gtatgccact aaaggcacca
     6721 aacatttttc ttctaatcaa tggaatgctt ttacagatgc agaaaaagaa gtgtggttaa
     6781 tgaaaagaag attagcattt tcttcttatg gagaggtaaa ctggtgcgaa gctttaggaa
     6841 ctgttttagc caatgacgaa gttgtgaatg gtgtaagtga aagaggaggg caccctgtta
     6901 taaaaaagaa aatgaggcag tggtacttaa gaattacagc ctatgcagat agacttttac
     6961 aaggtttaga aactgttgac tttagtgata gcatgaaaga aatgcaacgt aactggattg
     7021 gtaaaagtga aggagcagaa attaatttta aaattagtaa accctttaaa gatgatacta
     7081 ataacaataa agaatttagc gatgttataa aggtttacac tacacgacct gatactattt
     7141 ttggtgttga ctttatggtt atagccccag agcatgaaat ggttgaaact attatttctg
     7201 aagaacaaaa actatatgtt gaaaaatata tagcttatgt aaaaagtaga agtgatagag
     7261 aacgccaagc agagaaaaat atatcagggg tttttactgg ttcttatgct ataaatcctt
     7321 ttgatggtaa aaaaattcct atttggataa gtgaatatgt gttgataggt tatggcactg
     7381 gtgcaattat ggctgtacct tgtggcgatg aacgagattt taagtttgca aaacacttta
     7441 atattcctat tactaatatt attggaaacc attttaatgg attagaggct aaccctacta
     7501 aagaggccac tttagaaaac agtaattttt taaatggctt agtaatgaaa gatgctatta
     7561 ccattgctat taataaacta gaagaattgg gcattggtaa acgaaaggta aattataaaa
     7621 tgagagatgc tggctttagt aggcaacgct actggggcga accttttcct attatttgga
     7681 aagaaggaac acccacagca ttaagtgcca gccaactacc attagagcta cctcatgtag
     7741 aaaagtatag cccaggccct gaaggtgaag gaccactagc aaatattcaa aattggataa
     7801 atacacccga gggtaaaaga gaatcctcaa caatgcctgg ttatgctggt tcatcttggt
     7861 actttttacg ctatatggac cctgagaaca accaagaatt tgtaagtaga actgcaagtg
     7921 attattggaa tagcgttgac ttatatattg gtggtacaga gcatgctgtt gggcatttat
     7981 tatatagccg tatgtggact aaagtattgt acgacttagg ctatattggt tttgatgagc
     8041 catttaaaag gttagtaaat caaggaatga tacaaggtag cagcaggttt gtatatagaa
     8101 taaatggaac caatacctat gtttctaaaa acttaattag cgaaggtgac caatatttgt
     8161 atcaagaaaa aaaggtggat aaaatgcatg tagatgtaaa tatggttgat gggcttgaat
     8221 taaatattga acaatttaaa caatggcgta atggtgaaaa taataatgct gaatttattt
     8281 tagaaggtga aaaatatatc tgtggagttg aagtagaaaa aatgtcaaag tctaaataca
     8341 atactgttaa ccccgatgat ttatgtgtac aatatggtgc cgatactttt agaatgtacg
     8401 aaatgttttt aggccctgta gaacaaagca aaccatggga cactaaaggt attgaaggcg
     8461 ttcatcgctt cttaaaaaaa ttatggcgtt tattttatag cgaatctagt ttctcaaacg
     8521 gtattcaaac taacggaaat actattttta caaaggatgc tgctacttct gaagaactaa
     8581 aagtgttgca taaaaccatt aaaaaaatag atgaagatac agaacgtttt tcttacaata
     8641 cagctgttag tgcttttatg gtttgtgtaa acgaattggc agatttaaag tgctttaaaa
     8701 aagagatttt agaaaatgta ttaattctat tagcacctta tgcaccacat attgctgaag
     8761 aactatggaa tgttttaggc aacgaaggaa gtgtattaga tgctgcctat cctatttaca
     8821 atagcaacta cactattgaa agtagtaaag aatatcctgt aagcattaat ggaaaattaa
     8881 gaacaacaat gcattttgat attaatgcag aacaaaaaga tgtagaaccc gttgtgcttg
     8941 caaatgaaat tgtacagaaa tggttagaag gaaaaaatcc taaaaaaata atttttgtaa
     9001 aaaataaaat gataaacata gtagtttaat ttacacaact ataatacaca ttatatgttt
     9061 gaaaatgtaa ttacttattt ttctacctta gagcaacgcc ctttagaaag gttggcattt
     9121 ttagtagtgg ggttgttatt cttttggata atggaaggat atataccttt aattaccatg
     9181 cagtataaga aaaataagct taagcatgct ggcgtaaatt ttgcatttac tgttatacat
     9241 ttaataatac atactttttt agctttagca attattaaaa taagcgattg gtgtttagcc
     9301 aacaattttg gtttagtgta ttggattaaa accaatgtat tatttactat attaatttca
     9361 tttttaattc tcgatttttt tggtggttgg ctggtacata ttgtagaaca taaaatacct
     9421 ttattgtggc gttttcatgt aatacatcat tcagataata atgtagatgt aactacaggt
     9481 ttacggcatc atcctataga aagtgtaata cgtggcattt tcttttttgc aggcattgta
     9541 gtaagtggtg cacctatgta tgctgtaatg atttttcaaa ccattttagt tatttctacc
     9601 gcatttactc atgccaatat tgcattgcct ttatggttag ataaattatt aagttatttt
     9661 attgtatcgc caaatatgca caaagtacac catcattaca aacaaccttt taccgatagt
     9721 aattatggtg caatattaag tatttgggat aggctgttgg gtactttttc tcatttacac
     9781 cattctaaaa taaaatatgg tttagataat ttttacccca atgaaaaaga cgaagacttt
     9841 atgctattaa tgaaaaagcc ttttggtaaa atgactaaag aataaatata ttttttagct
     9901 tctaaaaatt tattcaatga tttatcagtt caaaaattat atacccgttg tgcaccaaag
     9961 ctcttttgta cacccacaag cagcagttac aggcaatgta attattggta aaaatgttta
    10021 tgtagcacct ggtgcagcca tacgaggcga ttggggtaaa ataattattg aagatggttg
    10081 caatattcaa gaaaactgta ctatacacat gtttcctggt attacagttg ttttaaaaga
    10141 ggctgctcat attggtcatg gagctattat acatggagcc actattggta aaaactgctt
    10201 aataggtatg aatgcggtaa taatggataa tgttgtttta ggaaatgaat gtattgtagg
    10261 tgctttaagt tttataaaag ccgatgaaaa atttgaggat agaagtttaa tagttggcaa
    10321 tcctgcaaaa aaaataaaag aagtaagtaa cgaaatgata gcttggaaaa ctgaaggaac
    10381 taaactgtat caacaattac caacagaatt gcatgcttat ttaaaagcag tggaaccttt
    10441 aagagaaatg ccttctaata gcaacaacga agagagtgtt tacaaaacat ggaatgaaac
    10501 taaataaatt gagacactat cccataaagt gtgtaagtaa aataattagg ggttcagctt
    10561 ttttat
//
LOCUS       DAOQPK010000061        10516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_3301_length_10516_cov_4.244814, whole genome shotgun sequence.
ACCESSION   DAOQPK010000061 DAOQPK010000000
VERSION     DAOQPK010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10516
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3301_length_10516_cov_4.244814"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="PLU36_04445"
     CDS             complement(<1..456)
                     /locus_tag="PLU36_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HMZ46031.1"
                     /translation="MATPKEQDYPAYYAQYLAKINAANILDLMSKYAGLIEQFIHEIP
                     DDKANFAYAKNKWTIKEVLQHLIDSERVFVYRAMRFARKDFIELEGYDENEYTKNAHA
                     NCRDFSILKQEFLLLRKTTDLFLLSLTEEDLQQKGIANNEPITVHAIVFI"
     gene            complement(456..2150)
                     /locus_tag="PLU36_04450"
     CDS             complement(456..2150)
                     /locus_tag="PLU36_04450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HMZ46032.1"
                     /translation="MKKVILFVFVLIISAKFSFSQKTVANGWHLLNYQQDSVYGIDLN
                     RAYAFLLEKNKKATPVIVAVIDSGIDTAHEDLKNILWKNTKEIPGNGIDDDKNGYIDD
                     IYGWNFLGNKDGRNIKKASSEKSRIFHTYKNKYENKELDTNLLSKDEKYIYKTWLRSA
                     AEINASTQEQANITYIELALKSLIKNDKIVREDMGKEEYTFEELEIYIPKTNDAKRAK
                     VGVLQIIKLFSLDSKSKNTEVIEEISEYVEGKKASFEEAEKAPFDYRADIVKDNTKDI
                     NDKNYGNNDVMASTPKHGTHVSGIIAAQRNNNLGMDGVADNVKIMTIRAVPDGDEYDK
                     DVALAIFYAVDNGAKVINMSFGKGFSPEKKWVDSAVQYAASKDVIILHAAGNDSKNID
                     SSENFPNPFMYYTGKRAPNYVTVGASSDPKISGSLAASFSNYGINTVDVFAPGDKIYS
                     TIPGGNKYGNLQGTSMATPVVAGIAALLRSYYPSLSAVQIKNIIEKSATIYDNSVATF
                     LPGTNDKTTLSELCRTGGLVNAYAAVQLAEKTVPEVNKVEENKLPIKPTKKVKKKN"
     gene            2275..4161
                     /locus_tag="PLU36_04455"
     CDS             2275..4161
                     /locus_tag="PLU36_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMZ46033.1"
                     /translation="MKNFKNIYFVLLLLTFSLTSYSQMINGRVINERGKAAANISVKF
                     TNKANTISTNKDGSFKIMATKLPDTLIFSAEGYESYKVIVTEKTVKDPDFSIVLLTKR
                     EKSSDPTVEGAGFLEGAEMLTMAGRGLVSAGTVSSSDTYTKTSDASKHKEVSTVSYGK
                     IKSTRAMGSASDGGKSKTYYYEDSVEVAKDQYETKLLTAGEVNDFSKWKMWEDYTDKD
                     FATYSNYWKLFAKKRYCVQVQTNNYYAAIGQKVYLLDANTLDTVWQAITDNTGKSELW
                     GRMNTTTSQNNNYIIVCEGESVKIKTPYLFHEGINRITLTKSCVARNNVEIAFVVDAT
                     GSMGDEIEYLKLELEDVLTKTFNRYNNLELKASAVFYKDRGDDYVTKHINFNNDLLKM
                     LNFIKLQQAGGGGDMPEALDAALEVAIDSLSWSSDARAKIMFVVLDAPAHDGTQNKIY
                     SLIQKAAAKGIRIVPIVCSGADKNTEFLMRSVALATNGTYLFLTNHSGVGDKHIEPTT
                     DAYSVELLNSLLPRIIQQMIYANTCVEQEKSEPIVKTNNNILNIKVSPNPTKGQVTIT
                     TTKPLKEIYIADFTGKILMRIPVNIKQTSWSINIAHFPAGTYIVKYVTTENKWGAEKI
                     ILVH"
     gene            4240..5775
                     /gene="guaA"
                     /locus_tag="PLU36_04460"
     CDS             4240..5775
                     /gene="guaA"
                     /locus_tag="PLU36_04460"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HMZ46034.1"
                     /translation="MTSKILILDFGSQFTQLIARCVREANIYCEIIPYNKKFTINNSL
                     KGIILSGSPFSVNDINNPEVDIQSFIEHVPILAVCYGAQLTAKMFGGRVDKSNKREYG
                     RAVLKIQQADTLFNSVSKNTQVWMSHSDSIVELPKGFEVLATTESIPVAAFKNTDLDV
                     NPLYGLQFHPEVYHSTDGKQMIQNFLINICKCSQDWTPASYVVETVEALKKQIGNAHV
                     IMALSGGVDSTVAAMLISKAIGNRLHGIFVDNGLLRKNEFEQVLESYKQLQLNVTGVN
                     AKQLFYDALKNKTEPEEKRKIIGKLFIDVFQEESKKVKEIKFLGQGTIYPDVIESVSV
                     HGPSATIKSHHNVGGLPDTMHLELVEPLRFLFKDEVRKVGLELGIPADLINRHPFPGP
                     GLAIRILGEITEDKVKLLQDADNIYIQNLKETNLYQKVWQAGTILLPVKSVGVMGDER
                     TYEFTVALRAVTSVDGMTADWAHLPYEFLADVSNEIINNVRGINRVVYDISSKPPATI
                     EWE"
     gene            5836..7038
                     /locus_tag="PLU36_04465"
     CDS             5836..7038
                     /locus_tag="PLU36_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46035.1"
                     /translation="MKHFLFFILFSQLTMFGSAQSNNSDSSQTKTFKVAIFAPIFLDS
                     AFNGTFFKISGNNSLPKTILPGLDFYNGVMMAIDSLQREKMPLEILFFDTKSKDLPMK
                     KILTNNVWDSVSLIIASFKDRHEIKPMADFAKLKKIPIISATYPNDGGVNDNPYFVLL
                     NSTLRTHCEGVYKYIQKNFSTNNLIYIKRHGRVENEIEKIFREIGEITPAIPLKYKTV
                     ILTDSLNQQDLRKVLDSSKTNVIICGSVNENFGIRLVKTLSTLKGTKSIAIGMPTWDG
                     IKEFNNLSMDEATKGVEIVYSSPYYFPRKEAFGKYITEQYKDKFFARASDWFLKGYET
                     TYNFCHLLVKYQQDFMQQLTSTEYKVFTDYNIQPLLNKNEVPSIDYFENKNLYFYRKQ
                     DGILKSVH"
     gene            7069..7941
                     /locus_tag="PLU36_04470"
     CDS             7069..7941
                     /locus_tag="PLU36_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HMZ46036.1"
                     /translation="MKRIADSELIINSRGAVYHLDITPDELADTVITVGDPFRVALVS
                     KYFDKIESKGQHREFVHTTGYIGKKRLTVVSSGIGPDNIDIVINELDALKNIDLNTRT
                     IKEVHTPLTIIRLGTSGSLQADIPVDSLVVGTHGLGLDNLLHFYRMEMNVEEQQLLQQ
                     FIAHTQLTGNIQPYISSGSASILKHFVNGFHSGITVTCPGFYGPQGRILRLGISNPTL
                     VDNLTSFSFGNHRISNFEMETSAIYGLGKLLGHHCLSINVIVANRVQKEFSKDSAASV
                     EKLLKNCLAVIEKI"
     gene            7958..8515
                     /locus_tag="PLU36_04475"
     CDS             7958..8515
                     /locus_tag="PLU36_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HMZ46037.1"
                     /translation="MRKLSMNELGRISVAEFKQAKKTPIIVVLDNIRSVYNVGSIFRT
                     ADAFLIEGICICGYSPTPEHKQMAKTALGATDTVDWLHYPSAIEAVQTLQQNGYKVFA
                     IEQADESISLENFNSNLYSKMAVVFGNEVEGIQQDVIEACNGCVEIPQLGTKHSLNVA
                     TAAGVVLWKLVESYITTSTIHKPNK"
     gene            8614..9789
                     /gene="mqnE"
                     /locus_tag="PLU36_04480"
     CDS             8614..9789
                     /gene="mqnE"
                     /locus_tag="PLU36_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HMZ46038.1"
                     /translation="MVDRINSIEVLINKEKNTALQTIAGKVVDNKRLSFDDGVLLFKE
                     ASLPFVGTLANFVRENKHGNKTYFNRNFHIEPTNICVFSCNFCSYSRLYAHREEGWEL
                     SLEDMLKIVKEYDNQPVTEVHIVGGVHPKLTLQFFADTIKAIKTHRPNLHVKGFTPVE
                     LDYMFKKAKLSIEEGMQFLKDAGLESLPGGGAEIFHQDIRSKICNDKVDADGWLKIHK
                     VAHQLGMHSNATMLYGHIEEFWHRVDHMEQLRQLQDETKGFNTFIPLKFRNKNNQMSN
                     VAESSIIEDMRMYAIARLYMDNFSHLKAYWPMLGRQNAQLSLSFGVNDIDGTIDDTTK
                     IYSMAGSEEQSPSMTTEELVQLIKQVKREPIERDTLYNEIKNYSFEQNVLKEELINT"
     gene            9836..10414
                     /locus_tag="PLU36_04485"
     CDS             9836..10414
                     /locus_tag="PLU36_04485"
                     /inference="COORDINATES: protein motif:HMM:NF023255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3347 domain-containing protein"
                     /protein_id="HMZ46039.1"
                     /translation="MKKLFFYGLFIFAVACNSDDKPVIEEKPAAPLSKSKNSDVFNQS
                     FKGLMDTYYHLKDNFITESDTMINTYAKQMQTMVDSLKLSEIKGEAGIVENAKMFTQN
                     ISNDLKNILASKDLLTKRKSFNVLSDNVYNLIKIVQYDREIVYHQHCPMAFEEGDANA
                     YWLSRSSDIKNPFRPTSMLTCGDVADSLDYSK"
BASE COUNT         3790 a         1510 c         1735 g         3481 t
ORIGIN      
        1 aataaaaaca attgcatgta cagttattgg ttcattgttt gcaatacctt tttgttgtaa
       61 atcctcttcg gttaatgaaa gtaaaaataa atcagttgtt ttcctcagta ataaaaactc
      121 ttgttttaaa atactaaagt ctctacaatt tgcatgagca tttttagtat attcattttc
      181 atcgtagcct tctaattcaa taaaatcttt tcttgcaaat cgcattgccc tatacacaaa
      241 aactctttct gaatctatta aatgttgtaa tacttcttta atagtccatt tgttttttgc
      301 ataagcaaag ttggctttat catctggtat ttcatgtata aattgttcaa ttaaacctgc
      361 atatttgctc attagatcca aaatatttgc agcatttatt tttgctaaat attgggcata
      421 atatgcagga taatcttgtt ctttaggagt agccattaat ttttcttttt taccttttta
      481 gtaggtttaa taggcaattt attttcttct actttattta cttctggtac agttttttct
      541 gcaagttgaa cagcagcata ggcattaact aaaccaccag ttctacataa ctcacttaaa
      601 gttgttttat catttgtacc tggcaaaaaa gttgctacag aattatcgta aatagttgca
      661 gatttttcaa taatattttt aatttgaaca gcacttaaac tagggtaata agaacgtaat
      721 aatgctgcaa taccagcaac aacaggcgtt gccatactag tgccttgtaa attgccatat
      781 ttattaccac caggtatagt agaataaatt ttatcgccag gggcaaaaac atctactgtg
      841 tttattccat agttgctaaa acttgctgct aatgaaccag aaattttagg atcgctactt
      901 gctccaacag ttacatagtt aggagctctt ttacctgtat aatacataaa tggatttgga
      961 aagttctcgg atgagtcaat attttttgaa tcgttgcctg cagcatgcag tataattaca
     1021 tctttagagg cagcatattg tactgcacta tccacccatt ttttttctgg cgaaaagcct
     1081 ttaccaaaac tcatattaat cacttttgct ccattatcaa ctgcataaaa aatagctaaa
     1141 gcaacatctt tatcgtattc atctccatct ggcacagctc taattgtcat aatttttaca
     1201 ttgtctgcaa ctccatccat acctaagtta ttgttacgtt gtgcagcaat aatgccacta
     1261 acatgtgttc catgttttgg agtagatgcc ataacatcat tattgccata attcttatca
     1321 ttaatatctt tagtattatc ttttacaata tctgctctat aatcaaatgg tgctttttct
     1381 gcttcttcaa aagatgcttt tttgccttct acatattcac ttatttcctc aattacctca
     1441 gtatttttac ttttagaatc taagctaaaa agtttaatta tttgcaatac accaactttt
     1501 gctcttttag catcatttgt tttgggtata taaatttcta attcttcaaa agtatattct
     1561 tctttaccca tatcttctct tactatttta tcattcttaa ttaagctttt taatgcaagt
     1621 tctatatagg taatatttgc ctgctcctgt gtacttgcat taatttctgc tgcacttctt
     1681 aaccaagttt tataaatgta tttttcatct ttagaaagca aattggtatc taactcttta
     1741 ttttcatatt tatttttata agtatgaaaa attctactct tttcactact ggctttttta
     1801 atatttctac cgtctttatt gcctaaaaaa ttccaaccat aaatatcatc tatataccca
     1861 tttttatcgt catcaatacc attgccagga atttctttag tattcttcca taaaatattt
     1921 tttaaatctt catgtgcagt atcaatacca gagtctataa ctgcaacaat aacaggtgtt
     1981 gcttttttat ttttttcaag aagaaaggca taggctcggt ttaaatcaat accataaaca
     2041 ctatcttgct ggtaatttaa caaatgccat ccattggcaa cagttttttg cgaaaaactg
     2101 aattttgctg aaatgattag aacaaaaaca aaaagtatta cttttttcat gtggtttatt
     2161 tcaaatttta ttaaagcaaa aatggcactt tataattgaa agaaatgata aaatattttt
     2221 ttggcaaatt ttatgtaatt aaaaaccttt ttgcatctgg tataaaattt tagtatgaaa
     2281 aactttaaaa atatatactt tgtactgttg ttgcttactt tttctttaac ctcttacagc
     2341 caaatgataa atggtagagt aataaatgaa cgaggcaagg cagcagccaa tataagtgta
     2401 aagtttacga acaaagccaa tactatatct acaaataaag acggtagctt taaaattatg
     2461 gctaccaaat tacccgatac attgattttt agtgcagaag gatatgaatc ctataaagtt
     2521 atagttacag aaaaaacggt gaaagatccc gatttttcta ttgtgctatt aactaaaaga
     2581 gaaaaatcat ctgatcctac agtagaaggt gcaggttttt tagaaggagc tgaaatgtta
     2641 acaatggcag gtagaggctt agtttcagca ggtacagtta gtagtagcga tacatataca
     2701 aaaacttccg atgcctctaa acacaaagaa gtttcaacag ttagttatgg taaaattaaa
     2761 agtacaagag ctatgggctc tgcatctgat gggggtaaaa gcaaaactta ttattatgaa
     2821 gatagcgttg aagttgccaa agaccaatac gaaactaaat tacttacagc aggcgaggta
     2881 aatgattttt ctaaatggaa aatgtgggaa gattatacag ataaagattt tgcaacctac
     2941 tctaattact ggaaactatt tgcaaaaaaa cgttactgtg tgcaagtgca aacaaataat
     3001 tactatgctg caattggtca aaaagtttat ttattagatg caaatacttt agatactgtt
     3061 tggcaagcta taacagataa tactggaaaa tcagagttat ggggcagaat gaatacaact
     3121 acatcgcaaa acaacaacta tataatagtt tgtgaaggag aaagtgtgaa aattaaaact
     3181 ccgtacttgt ttcatgaagg tattaataga attactttaa ctaaaagttg tgtagcaagg
     3241 aacaatgtag aaattgcatt tgttgttgat gcaacaggaa gtatgggaga tgaaattgaa
     3301 tatttgaaat tagaattaga agatgtatta actaaaacat ttaacagata taataattta
     3361 gaattaaagg catcggctgt tttttataaa gatagaggag atgattatgt aacgaaacac
     3421 atcaatttta ataacgactt attaaaaatg ttaaatttta ttaaattaca gcaagcaggt
     3481 ggtggtggtg atatgccaga agctttagat gctgctttag aagtagcaat agatagcttg
     3541 agttggagca gtgatgctag agctaaaata atgtttgtag ttttagatgc acctgcacat
     3601 gatggaacac aaaataaaat atacagttta attcaaaaag ccgctgctaa aggaattcgt
     3661 attgtaccaa ttgtatgtag tggtgctgat aaaaatactg aatttttaat gcgttctgta
     3721 gctttggcaa ctaatggtac ctatttattt ttaacaaatc atagtggagt aggagataaa
     3781 catattgagc ctacaacaga tgcatatagt gtagaattat taaatagttt attgcctaga
     3841 attattcaac aaatgattta tgcaaatact tgtgtagagc aagaaaaatc tgaaccaatt
     3901 gtaaaaacta acaataatat tttaaatatt aaagttagtc caaacccaac caaagggcag
     3961 gttactataa ctactaccaa gcctttaaaa gaaatttata ttgccgattt tacaggtaaa
     4021 attttaatgc gtattcctgt aaacataaaa caaacaagct ggtctataaa tattgctcat
     4081 tttcctgctg gtacttatat tgtaaaatat gttacaacag aaaacaaatg gggggctgaa
     4141 aaaattattt tagtacatta acctcaaaaa tgtgtattta acaaaaggct tattctaatt
     4201 aggataagcc ttttttctgc aaaggcttat cttcgcagca tgacaagtaa gattctaatt
     4261 ttagattttg gaagccaatt tacacagctt attgctcgtt gtgttagaga agcaaatatt
     4321 tattgtgaaa ttattcctta caataaaaaa tttactataa acaattcttt aaaaggtatc
     4381 atacttagtg gcagcccatt tagtgtaaat gatataaata atcctgaagt tgatatacaa
     4441 tcttttattg agcatgtacc tattttagct gtatgctatg gggcacaact tactgcaaaa
     4501 atgtttggtg gtagagtaga taaaagtaat aaaagagaat acggaagggc agtattaaaa
     4561 attcaacaag cagatacctt atttaatagt gtttcaaaga atactcaagt atggatgagc
     4621 catagcgata gtattgtaga acttcccaaa gggtttgaag tattggcaac aacagaaagt
     4681 attcctgtag ctgcttttaa aaataccgat ttagatgtaa atccattata tggcttacaa
     4741 ttccatccag aagtatatca ttcaactgat ggaaagcaaa tgattcaaaa ctttttaatc
     4801 aatatttgca aatgttcaca agattggaca cctgcttcgt atgtagtaga aactgtagaa
     4861 gccttaaaaa agcaaattgg caatgcacat gttattatgg cattaagtgg tggtgttgat
     4921 agcactgttg cagcaatgct aattagtaaa gcaattggta atagattaca tggcattttt
     4981 gtagataatg gactgctaag aaaaaatgaa tttgaacagg ttttagaatc ttataaacaa
     5041 ttacaattaa acgtaacagg tgttaatgct aagcaattat tttatgatgc attaaaaaac
     5101 aaaacagagc ctgaagagaa aagaaaaatt attggtaaac tatttattga tgtttttcag
     5161 gaagagtcaa aaaaagtgaa agaaataaaa tttcttgggc aaggtacaat ctatccagat
     5221 gttattgaaa gtgtaagtgt tcatggtcct agtgcaacaa ttaaaagcca ccataatgta
     5281 ggtggcttgc cagatacaat gcatttggag ttggtagaac ccttgcgttt tttatttaaa
     5341 gatgaagtaa gaaaagtagg cttagaatta ggcattcctg cagatttgat aaacaggcac
     5401 ccatttccag gaccagggtt agccattaga attcttggtg aaatcactga agataaagta
     5461 aagcttttgc aagatgccga caatatatat attcaaaatt taaaagaaac taacctttac
     5521 caaaaagtat ggcaagcagg aacaattttg ttgccagtaa aaagcgttgg agtaatgggt
     5581 gatgaaagaa cctacgaatt tactgttgct ttaagagctg taacaagtgt agatggtatg
     5641 actgcagatt gggcacattt gccctacgag tttttagcag atgtatctaa cgaaattatt
     5701 aataatgtaa gaggaattaa cagagttgtt tacgatatta gcagtaagcc accagcaaca
     5761 attgagtggg agtaaacttt ttatcttttt tggcactata tttatacctg tttataagta
     5821 attattattt tactaatgaa gcatttttta ttttttattt tattttctca attaaccatg
     5881 tttggtagtg cacaatccaa taactcggat agcagccaaa caaaaacttt taaggtagca
     5941 atctttgctc caattttttt agactctgca tttaatggca ccttttttaa aatttcagga
     6001 aataatagct tgcctaaaac aatattacca gggctagatt tttataatgg tgtaatgatg
     6061 gctattgaca gtttacagag agaaaaaatg ccattagaaa ttttattctt tgatacaaag
     6121 agtaaagatt tacctatgaa aaaaatttta accaacaatg tttgggatag tgtttctcta
     6181 attattgcat cttttaaaga taggcatgaa ataaaaccta tggcagattt tgccaagcta
     6241 aaaaaaattc caattatatc tgctacctat cctaacgatg gtggtgtaaa cgataatcca
     6301 tattttgttc tattaaattc tactttacgt acccattgtg aaggagttta taaatatatt
     6361 caaaaaaatt tctcaactaa taatttaatt tatataaaaa ggcatggacg tgttgaaaat
     6421 gaaattgaaa aaatatttag agagatagga gaaataacac cagcaattcc attaaaatat
     6481 aaaacagtta ttttaaccga ttctctaaac cagcaagatt tgagaaaagt tttagatagt
     6541 tctaaaacaa atgtaattat ttgtggtagt gtaaatgaaa actttggtat aagattagta
     6601 aaaacattga gtacattaaa aggtacaaaa tcaattgcta ttggcatgcc aacatgggat
     6661 ggaataaagg aatttaataa tttatcaatg gatgaagcaa caaaaggtgt agaaatagtt
     6721 tattcttctc catattattt tccaagaaaa gaagcttttg gaaaatatat aacagaacag
     6781 tataaagata aattttttgc aagagccagc gactggtttt taaaaggtta tgaaacaaca
     6841 tacaactttt gtcatttgct agttaagtat cagcaagatt ttatgcagca actaaccagt
     6901 accgaatata aagtatttac agattataat attcaaccat tactcaacaa aaatgaagta
     6961 ccgagtatag attattttga aaataaaaat ttatactttt atagaaaaca agatggaatt
     7021 ttaaaatcgg tacactaaat aataactaaa aaataaaaca aattttatat gaagagaata
     7081 gcagattcag aattaattat taatagtaga ggtgctgtgt atcatttaga tattacacca
     7141 gatgaattgg cagatactgt aattacagtt ggcgacccat ttagagttgc tcttgttagc
     7201 aaatattttg acaagattga atcaaaaggc cagcaccgag aatttgttca taccacaggt
     7261 tatattggga aaaaaaggct tactgttgtt tcgtcgggca ttggacctga taatatagat
     7321 attgttatta acgaattaga tgcattaaaa aatatagact taaacacaag aactataaaa
     7381 gaagtgcata caccattaac cattattcgc ttaggcactt ctggctcttt gcaagcagat
     7441 atacctgtag atagtttggt tgttggcaca catggtttag ggcttgataa tttactccat
     7501 ttttatagaa tggaaatgaa tgtagaagaa caacaactat tgcaacaatt tattgcacat
     7561 acgcaactta caggcaatat tcagccttac atttcatctg gtagtgcaag cattttaaaa
     7621 cattttgtaa atggttttca tagcggtata actgttacct gccctggttt ttatggtcct
     7681 caaggtagaa tattaagatt aggaatttcc aatccaaccc ttgtagataa tttaacaagt
     7741 tttagttttg gcaaccatcg tatcagcaat tttgaaatgg aaacaagtgc catttatggg
     7801 ttgggtaaat tacttggcca tcattgctta agtattaatg taattgttgc caatagagta
     7861 caaaaagaat ttagcaagga cagtgcagca tcggttgaaa aattattaaa aaattgctta
     7921 gctgtaatag aaaaaatata aagccaatct agtttgtatg cgtaagctaa gtatgaatga
     7981 attgggaaga atttctgttg ctgagtttaa acaagcaaaa aaaacgccaa tcatagttgt
     8041 attagacaac attagaagtg tatataatgt aggaagtatt tttagaacag cagatgcatt
     8101 tttaatagaa ggtatttgca tttgtggcta ttcgccaaca cccgaacata agcaaatggc
     8161 taaaacagct ttaggtgcta cagatactgt tgattggctt cactacccat cggcaataga
     8221 agctgtacaa accttacaac aaaatggcta caaagttttt gccatagaac aagccgacga
     8281 aagtatttct ttagaaaatt ttaattctaa tttatattca aaaatggcag ttgtttttgg
     8341 taatgaggta gaaggtattc agcaagatgt aatagaggct tgcaatggtt gtgttgaaat
     8401 tccacaactt ggtactaagc actctttaaa tgttgcaaca gctgctggag ttgttttatg
     8461 gaagttggta gaatcatata ttacaacctc tacaatacat aaacctaata aatagcaacc
     8521 ccttcaagtt atttataaac ttaattattt aatcactttt atctttctaa ttaatattgt
     8581 acacttagct ttgctttgga taaattaaaa attatggtgg atagaattaa tagtattgaa
     8641 gttttaataa ataaagaaaa aaatacagca ttacaaacta ttgcaggaaa agtggtagat
     8701 aataaaaggt taagttttga tgatggtgta ttacttttta aagaagcctc cttaccgttt
     8761 gtgggtacat tagctaactt tgtaagagag aataaacatg gcaacaaaac ttattttaac
     8821 cgcaattttc atatagagcc tacaaatatt tgtgtattta gttgtaattt ttgtagttat
     8881 tctcgtttat atgcacatag agaagaagga tgggagttaa gtttagaaga catgttgaaa
     8941 atagtaaaag aatacgataa tcaacctgtt accgaagtgc atattgttgg aggtgtacac
     9001 ccaaaattaa ctttacaatt ttttgccgat actattaaag caattaaaac ccaccgtcct
     9061 aatttgcatg taaaaggctt tactcctgta gagcttgatt atatgtttaa aaaagcaaaa
     9121 ctttctatag aagaaggaat gcagttttta aaagatgcag gattagagtc tttacctggt
     9181 ggaggtgcag aaatttttca tcaggatatc cgatcaaaaa tatgcaacga taaagtagat
     9241 gctgatggat ggttgaaaat tcataaagta gcccaccaat taggtatgca ttcaaatgct
     9301 accatgttat atggccatat tgaagaattt tggcataggg tagatcacat ggagcaatta
     9361 cgccaattac aagatgaaac taaaggattt aataccttta ttcctttaaa atttaggaat
     9421 aaaaataacc aaatgagcaa tgtggcagaa tcttccatta tagaagatat gaggatgtat
     9481 gcaattgcca gattgtatat ggacaacttc tctcatttaa aagcatattg gcctatgctt
     9541 ggcaggcaaa atgcacagct ttccttaagt tttggtgtaa atgatattga tggaactata
     9601 gacgatacca ctaaaatata ttccatggca ggtagtgagg agcaaagccc atcaatgaca
     9661 acagaagaat tagtgcaact tattaaacaa gttaaaagag aacccattga aagagacaca
     9721 ctctataacg aaataaaaaa ctatagtttt gaacaaaatg ttcttaaaga agaactaata
     9781 aatacttaaa cttttataaa cattaaatta ttatcttaaa ataaaataca cagttatgaa
     9841 aaaattattt ttttatgggt tatttatttt tgctgttgca tgtaacagcg atgataaacc
     9901 agttattgaa gaaaaaccag cagcaccatt aagtaagagt aaaaattctg atgtgtttaa
     9961 tcaatcattt aaaggattga tggacactta ttatcattta aaggataatt ttattacaga
    10021 gagtgataca atgataaata cttatgcaaa acaaatgcaa acaatggtag atagtcttaa
    10081 gctgagtgaa attaaaggcg aagccggaat tgtagaaaat gcaaaaatgt ttacacaaaa
    10141 tatttctaat gatcttaaaa atatacttgc ttctaaagat ttgctaacta aaagaaaatc
    10201 atttaatgtt ttatcagaca atgtttacaa tctaataaaa attgttcaat acgatagaga
    10261 aattgtttac caccaacatt gcccaatggc ttttgaagaa ggagatgcaa atgcttattg
    10321 gctaagccgt tcttctgata ttaaaaaccc ttttcgccct acctctatgc ttacttgtgg
    10381 agatgtggca gattcattag attattcaaa ataatggaaa ggatttgcca acaaaaaagg
    10441 gctgcaaatt tatttgcagc cgacactatc ccataaagtg tgtaagtaaa ataattaggg
    10501 gttcagcttt tttata
//
LOCUS       DAOQPK010000062        10210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_3456_length_10210_cov_4.950172, whole genome shotgun sequence.
ACCESSION   DAOQPK010000062 DAOQPK010000000
VERSION     DAOQPK010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10210
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3456_length_10210_cov_4.950172"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="PLU36_04490"
     CDS             complement(<1..470)
                     /locus_tag="PLU36_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HMZ46040.1"
                     /translation="MYNNISRINIKNIIAVCIIWLSSILPNFSFSTVLRVGKEADFKT
                     IKSAIKHAQNGDTVLISKAIYKEGNIVIDKPISIIGEDYPILDGEFEYEILSIKSNNV
                     VIKKLQLQNSGRSSMNDLSAIKIYENKYVVIENNKLINNFFGMYLQYAKHCLVK"
     gene            complement(460..1494)
                     /locus_tag="PLU36_04495"
     CDS             complement(460..1494)
                     /locus_tag="PLU36_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HMZ46041.1"
                     /translation="MHTTKISSLSKMLLFIGALFLIISLFVPIWSIYLDAPQYPEGLS
                     LKIWANAIAGDVDIINGLNHYIGMKTLHTKDFIEFAILPYIIVGFAILFALTALMGKK
                     KLLYIIFTAFVLFGIVAMADFYRWEYDYGHNLDPNAAIKVPGMAYQPPLIGFKQLLNF
                     GAYSIPSIGGWLFISAGLLSLTAVLKETKFLKKFSKSKSTLAVLLPMIVLFSSCNNQK
                     AEPISLNKDACTFCKMSIADGKFGAEIITKKGRVFKFDDLVCMNGYVSENNATEVKKY
                     FIADYTKDNQLIDATTAFFVQHEKMRSPMGGNIAAFATKEEAEKTATEYKTALLNWGT
                     LNKQISKNVQ"
     gene            complement(1863..3827)
                     /gene="nosZ"
                     /locus_tag="PLU36_04500"
     CDS             complement(1863..3827)
                     /gene="nosZ"
                     /locus_tag="PLU36_04500"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HMZ46042.1"
                     /translation="MITKKIAVGLLAVAAVYFTSCKPKGSSSAVSGDAASKTYVAPGK
                     YDEFYNFVSGGFSGQLSVYGLPSGRLLRVIPVFSVDPEKGWGYSEETKPMLNTSHGFV
                     PWDDLHHVEMSMTDAMPNGKWVFVNGNNTPRLARVDLKTFRTAEIIELPNSGGNHSSP
                     FGTENSEYIVAGTRFSVPADTDNGDVPINTYKQNFKGHISFVSVNKDNGEMNIAFQLK
                     TPGVNFDLARTGKGKSSGWFFFSTYNTEQANTLLEVNASKNDKDFIMAVNWKKAEEYI
                     KAGKGKKQSVRYAHNKFNEETHTATSEIKNEVTVLDVAEFPDIVYMIPCPKSPHGCDV
                     NPTGEYIVGSGKLAALLPVFSFEKIQKAIADKAFEAEKYNGIPVIKYEAALYGEVQKP
                     GLGPLHTEFDAEGNAYTSFFVSSEVVKWNVKDLKIIDRKPTYYSVGHLMVPGGNTKKP
                     YGKYLVAYNKITKDRYLPTGPELSQSAQLFDISGEKMQLILDFPTIGEPHYAQAVPAD
                     LIKNNQVKIFEIAKNKHPYVAKGEGETKVVREGNKVHVYITAVRSHFAPDNIEGVKMG
                     DEVYFHVTNLEQDWDVPHGFGVKGANNGELLIMPGETTTLKWVPDRVGIFPIYCTDFC
                     SALHQEMQGYVRVSPKGSNVPLTWSLGDKK"
     gene            complement(3894..4388)
                     /locus_tag="PLU36_04505"
     CDS             complement(3894..4388)
                     /locus_tag="PLU36_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMZ46043.1"
                     /translation="MRKKSLMMLLLVMPLFYVACSGGKTENNASTEPTEGPTTTTETA
                     DASSYDPKRGEGKYDEKNVDVTKFDAALAGQGKKVAETKCLSCHKMTDEKLVGPGWKG
                     VTQRRLPHWIMNFITNPDPMIDKDPEVQAQLEICLVRMPNQNLAENEAREILEYMRQN
                     DGAK"
     gene            4693..5352
                     /gene="purQ"
                     /locus_tag="PLU36_04510"
     CDS             4693..5352
                     /gene="purQ"
                     /locus_tag="PLU36_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HMZ46044.1"
                     /translation="MKFGVVIFPGSNCDRDMQYTLENDLNKEVIMLWHKDKDLSMFST
                     NDCIVLPGGFSYGDYLRCGAIARFSPMMQSVIEFANKGGKVLGVCNGFQILCESGLLP
                     GVLLQNKNQKFICNNVFIKNKNSGALKIPIAHGEGRFYADNDTLDSLEKNNQIIFSYC
                     NEVGEIEQQYNPNGTLRNIAGISNNFGNVFGMMPHPERATNYQLQNTDGIKVFEILGL
                     N"
     gene            5373..5825
                     /locus_tag="PLU36_04515"
     CDS             5373..5825
                     /locus_tag="PLU36_04515"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HMZ46045.1"
                     /translation="MKNLILVLAGVIFTFSVQAQKAKNPVSFSYKAVKKTDGTVDIIA
                     LATIEKGWHIYSQNTDKGGPVPTNIAFKLNPLAKKTGKVLEKGKVEKTFDKTFGVNVM
                     YYSNQVQFIQNFKIKNGIKTSIYGTVEYMVCDDEMCLPPKKENFEVKI"
     gene            5868..7814
                     /locus_tag="PLU36_04520"
     CDS             5868..7814
                     /locus_tag="PLU36_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HMZ46046.1"
                     /translation="MKKVILFLFLCVSSYFVFAQSENPLVWKVSYKKIDTATFTITAS
                     TTIPAAWHLYGKNETIEELTSVQFITADFENDELIGEPKFNTSPTVIKDAIFENQNLN
                     VYTGEITVTQNVKINGLIPNKLKGTVEAFIAKGDEFVPTSFVYEVSLEGGVAANTDAQ
                     KILLKSVDLNKPAAQCGHVEEVKNSSILKVFFLGILGGLIALLTPCVFPMIPVTVSFF
                     TKRSANHKEAVKNGTLYGVFIFLIYIAASIPFHLISNIQPEILNNISTNAWLNIVFFA
                     IFIFFAISFFGFFEISLPSSIAGKADSKGSLGSIGGIFFMALTLTIVSFSCTGPILGS
                     LLVGTASGGAMELTAGLAGFGIALALPFALFAVFPNWLKSLPKSGGWLDTVKKVLAFA
                     ELAFAIKFLSNADTVMHWGLLKREVFIGLWILISIGLVLYLFGFLRLPHDDRTAKISV
                     GRKIFGLIALAFTIYLIPGLTNTKHANLKLLSGFPPPKNYSIYGKESAHHKGLEANIS
                     NNYLKALQMAKEQNKPLLIDFTGWACVNCRKMEENIWTVPEVKQFITDHFILLSLYVD
                     DKEKLPVADRFTYKTKSGFEKDIVTVGDKWSTFQTENFGQTTQPLYVILDTEEKLLNN
                     PIGYTPSYNEYLKWLQCGKNAFQK"
     gene            7877..8263
                     /locus_tag="PLU36_04525"
     CDS             7877..8263
                     /locus_tag="PLU36_04525"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HMZ46047.1"
                     /translation="MMKLNSVGIGTRILNFLVDIILIFLLAYVAFKIHKWYVFYYKIT
                     YINFGWFFFGTMFFYCLLFETFFAKTPGKWFTQTKVVNLSGNKPSFFAIVLRSFIRLT
                     IIDVFFIPFIDKTLHDYVSKTVVVET"
     gene            8767..>10210
                     /locus_tag="PLU36_04530"
     CDS             8767..>10210
                     /locus_tag="PLU36_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46048.1"
                     /translation="MKKFLLLFTLLVTSFSSINAQTTVIIGNQGSQVAGTGPLSYNSY
                     ARFQTIYTAAELSAAGMVANSEITDIAFFITSSSNVPIKDYTIRMGHTTATNFSSPLS
                     VPLTTVRNPADYTPIRNPSDATWDTSLNLDNHFIWNGYDNIGIDICSGSSILAYNNTV
                     ALYPYTKGHRSENTSCQYLPFDTYNYRPAIQLTFITTCTTPTALSIGAITATSASATF
                     TSAGTAFIVEYGAAGFTPGTDSTAGVGGTVVTGSASPIALIGLTGSTTYDVYVRQNCT
                     NSGAGYSNNSIKATFTTLCMPGGDEVSYGNGSWEGYVYTWTGSPSFNSPNYKGNITEN
                     EQFDRDNGAGAIAGLTTNLCSPIPSDGYTVRYKMTKNFPAGYYKFTVGGDDGVRLSID
                     GGSTWLVNVWNVQSYTIYSSPTVFLNGSTNLVLEYYENAGDSRISFSYTIPCSTPTAL
                     SAGGVTNNSANIGFTSAGTAFIVEYGAAGFT"
BASE COUNT         3475 a         1600 c         1535 g         3600 t
ORIGIN      
        1 ttttttacta agcaatgttt tgcatattgt agatacattc caaaaaaatt attgattagt
       61 ttattatttt caattacaac atatttattt tcataaattt taatggcact taaatcgttc
      121 atgctgcttc ggccactgtt ttgtaactgt aattttttta ttactacatt gttgctttta
      181 atagataaaa tttcatactc aaattcacca tccagtattg gataatcttc gccaataata
      241 cttataggtt tatctataac aatattgcct tctttatata ttgcctttga aattaaaaca
      301 gtatctccat tttgtgcatg ttttattgca cttttaatag ttttaaaatc agcttcttta
      361 cctactctta aaactgttga aaatgaaaag ttgggtaaaa tgctgcttaa ccatattata
      421 caaacagcaa taatattttt tatattaatc ctgctaatat tattgtacat ttttagaaat
      481 ctgtttattt agtgtacccc aatttaacaa ggctgttttg tattctgttg cagttttttc
      541 tgcttcttct ttggttgcaa atgcagcaat attgccaccc attgggctac gcattttttc
      601 atgttgcaca aaaaatgctg ttgtagcatc tattaattga ttatcttttg tataatctgc
      661 tataaaatat ttcttaactt ctgttgcatt attttcactc acatatccat tcatacaaac
      721 caaatcatca aacttaaata ctcttccttt tttagtaatt atttcggcac caaattttcc
      781 atcggcaata gacattttac aaaaagtgca tgcatcttta tttaaagaaa ttggttcggc
      841 tttttgatta ttgcaagaag aaaacaaaac tatcattggt aacaaaacag ccaatgtaga
      901 tttactcttg ctaaactttt ttaaaaactt agtttctttt aaaactgcag ttaacgataa
      961 taaaccagca gaaataaaaa gccaacctcc aatgcttggt attgaatatg caccaaagtt
     1021 tagtaattgt ttaaacccta ttaatggtgg ttggtaagcc attcctggaa ctttaattgc
     1081 agcattgggg tctaaattgt gcccataatc gtactcccat ctataaaaat ctgccatggc
     1141 aacaatacca aataaaacaa aagctgtaaa aattatatac aataatttct ttttacccat
     1201 taaagctgtt aatgcaaata aaatagcaaa cccaacaatt atataaggta aaattgcaaa
     1261 ttcaataaaa tctttagtat gtaaagtttt catgccaata taatggttta acccattaat
     1321 aatatcaaca tcgccagcaa tagcattagc ccaaattttt aaactcaaac cttctggata
     1381 ttgaggagca tctaaataaa tactccagat aggaacaaat aatgaaataa taagaaataa
     1441 ggcaccaata aaaagtaaca ttttagaaag ggatgaaatt tttgtagtat gcattatata
     1501 aaatttatat ggtttaaggg taaattttga gttgcaaaat tatcattata tataataata
     1561 ccctatgacc tcaatcatga aactgcttaa agaaaactag aattataatt tatactcttt
     1621 tgagaaaagg ctttaaaaaa aatattaaaa agttagagtt attaaaccta aagcaatagg
     1681 taaaataaaa ttaaattaca tttattgggg cgttgttatt aacagctaga aatggaacaa
     1741 aagctattga aaaaaatatt taaacaattt tctttatttt actgcggaat aactttataa
     1801 aaactaaaat gctgcctaaa aatagtaggc agcattttaa tatttaacta ataaaaaaag
     1861 acttattttt tatcacctaa actccaagtt aaaggtacat tactaccttt aggagaaact
     1921 cttacataac cttgcatttc ttggtgtaat gcagagcaaa aatctgtaca ataaattggg
     1981 aaaataccta ctctatctgg tacccatttt aatgtggtag tttcaccagg cataataagt
     2041 aactcgccat tattagcacc ttttacacca aagccatgtg gtacgtccca atcttgttct
     2101 aagttggtta catgaaagta aacttcatct cccattttaa caccttctat attatctggt
     2161 gcaaagtgag aacgaacagc agtaatgtaa acatgtactt tatttccttc tcttacaacc
     2221 ttagtttcac cttctccttt agctacataa gggtgtttat ttttagcaat ctcaaatatt
     2281 tttacttggt tatttttaat taaatcggca ggtaccgctt gtgcatagtg tggttcgcca
     2341 atggttggga agtctaaaat taattgcatt ttttctccac taatatcaaa taactgagca
     2401 ctttgagaaa gctctggacc agtaggtaaa taacgatctt tagtaatttt gttataagct
     2461 actaaatatt taccataagg ttttttagta ttaccacctg gaaccattaa gtgaccaaca
     2521 gaatagtaag ttggcttacg atcaataatt tttaaatctt ttacattcca ttttactact
     2581 tcagaagaaa cgaagaagga agtatatgca ttaccttcag catcaaactc tgtatgcaaa
     2641 gggcctaatc ctggtttttg tacttcgcca tataaagcag cttcgtattt aataacagga
     2701 ataccattgt atttttcagc ctcaaatgct ttatctgcaa tagctttttg aattttttcg
     2761 aagctaaata caggtaataa agctgctaat ttaccactac ctacaatgta ttcacctgtt
     2821 gggtttacat cgcaaccatg tggagattta gggcaaggaa tcatgtaaac gatatctgga
     2881 aattcagcaa catctaaaac tgttacttca tttttaattt ctgaagtggc agtatgtgtt
     2941 tcttcattaa atttattatg ggcatatctt acagattgtt ttttaccttt tcctgcttta
     3001 atatactctt ctgctttttt ccagtttaca gccatgataa agtctttatc atttttagaa
     3061 gcatttactt ctaacaatgt gttagcttgc tctgtattgt aagtactgaa gaagaaccaa
     3121 ccactagatt tacctttacc tgtacgtgca aggtcaaagt ttacacctgg tgtttttaat
     3181 tggaaagcaa tattcatttc gccattatct ttattaacac ttacaaagct aatgtgacct
     3241 ttaaaatttt gcttgtaagt attaattggc acatcaccgt tatctgtatc tgcaggaaca
     3301 ctaaaacgtg taccagcaac aatatattca gaattttcag taccaaatgg cgaactgtga
     3361 ttaccaccac tattaggtaa ttctataatt tcggcagtac ggaaagtttt taaatctact
     3421 cttgctaaac gaggggtgtt attaccattt acaaataccc atttaccatt aggcattgca
     3481 tctgtcatag acatttcaac gtggtgtaaa tcatcccaag gtacaaaacc atgagaagta
     3541 tttaacattg gcttagtttc ttcgctatat ccccagcctt tttcaggatc aacagagaat
     3601 acaggaatta cacgtaataa acgaccactt ggtaagccat aaacacttaa ttggccacta
     3661 aagccaccag atacgaagtt ataaaattca tcgtatttac ctggtgctac ataagtttta
     3721 gaagcagcat cgccacttac tgcactacta gagccttttg gtttacacga agtaaaataa
     3781 acagcagcta cagcaagtaa gccaacagct attttttttg ttatcataat ttatttttta
     3841 aggatttaag aatttgaaaa ttggttgtat aaaatgagct atctaaatat tatttattta
     3901 gcaccatcat tttgacgcat atactctaat atttctctag cttcattttc agctaaattt
     3961 tggttaggca ttctaactaa acaaatctct aattgtgctt gtacttctgg gtctttatca
     4021 atcattgggt ctgggttggt aataaagttc ataatccaat gtggtaatct tctttgtgtt
     4081 acacctttcc aacctgggcc aactaatttt tcatcggtca ttttatgaca agataaacat
     4141 ttagtttctg ccactttttt accttgtcct gctaaagcag catcaaactt ggtaacatct
     4201 acattctttt catcatactt cccttcacca cgtttaggat catagctaga agcatcagca
     4261 gtttctgtag ttgtagtagg gccttcagta ggctctgtag atgcattatt ttcggtttta
     4321 cctccactac atgcaacata gaataaaggc attactaaaa gtagcatcat taacgatttt
     4381 tttctcataa ttttcaatga tttaaaattt tatgcaaaag taaattgtgt atgttatttt
     4441 aaggtatgac ttaagtcata tttaagaaaa atatttattt attttttaaa ctatttttcc
     4501 atataacacc acttaaaaac agttgtgtga aattattaaa aacaaaagta taaaaatata
     4561 ttaagcgttg cttattttct tttgttatag ctgtgtattt attaaagaaa ttaaaaagtg
     4621 tataattgtt ttcgtaaatc aaatatttaa tacagttaat gcaagtacct ttgccaccaa
     4681 tttatacaaa ttatgaaatt tggagttgtt atttttccag gctctaactg cgatagagat
     4741 atgcagtata ctttagaaaa cgatttaaac aaagaagtaa ttatgctttg gcataaagat
     4801 aaagatttaa gtatgttttc tactaacgat tgtattgttt taccaggtgg cttttcttat
     4861 ggcgattatt tacgctgtgg agctattgca aggtttagcc caatgatgca aagtgttatt
     4921 gaatttgcca ataaaggtgg aaaggtttta ggagtatgta atggctttca aattttatgc
     4981 gaaagtggtt tactgccagg cgttttatta caaaataaaa atcaaaaatt tatttgcaat
     5041 aatgtattta tcaaaaataa aaattcagga gctttaaaaa tacctattgc acatggcgag
     5101 ggaagatttt atgctgataa tgacacttta gatagtttag aaaaaaacaa ccaaattatt
     5161 tttagttatt gtaatgaagt tggtgaaata gaacaacaat ataatcctaa cggaacttta
     5221 agaaatattg ctggtataag taataacttt ggcaatgttt ttggaatgat gcctcatcct
     5281 gaaagagcta caaactacca actacaaaac accgatggaa taaaagtatt tgaaatttta
     5341 ggacttaatt aattataaat ttaaacccaa acatgaagaa tttaatttta gtattagcag
     5401 gtgtaatttt tacattttct gtacaggcac aaaaagcaaa aaatcctgta agtttttctt
     5461 acaaagcagt gaaaaaaaca gatggaactg tggatattat tgcattggct acaattgaaa
     5521 aaggctggca tatatattct caaaatacag ataaaggcgg cccagtacct accaatattg
     5581 cttttaagtt aaaccctctt gcaaaaaaaa caggtaaggt attagaaaaa ggtaaagtag
     5641 aaaaaacatt tgataaaacc tttggagtta atgtaatgta ttatagcaat caggtacagt
     5701 ttattcaaaa ttttaaaatt aaaaacggaa ttaaaacatc tatttatggt actgtagagt
     5761 atatggtttg cgatgatgaa atgtgtttgc ctcctaaaaa agaaaacttt gaagtaaaaa
     5821 tttaatgatt acttcagttt tttactacct ttttaaaaca ccagtacatg aagaaagtta
     5881 ttctgttttt gtttttatgt gttagcagtt attttgtatt tgcacaaagt gaaaatcctt
     5941 tagtttggaa agtaagttat aaaaaaattg ataccgctac ttttaccatt acagcaagta
     6001 caactatacc tgcagcatgg catttatatg gcaaaaatga aaccattgaa gaactaacca
     6061 gtgtacaatt tattactgct gattttgaaa atgatgaatt aataggagaa cctaaattta
     6121 atacttcgcc cacagttatt aaagatgcca tttttgaaaa ccaaaactta aacgtttata
     6181 caggagaaat tacagttact caaaatgtaa aaataaatgg ccttatacct aataaattaa
     6241 aaggtactgt ggaggcattt attgctaaag gagatgaatt tgtacctacc tcttttgttt
     6301 atgaagtaag cctagaaggt ggtgtagctg ctaatacaga tgcacaaaaa atattattaa
     6361 aatctgttga tttgaataaa ccagcagctc aatgtggcca tgtggaagaa gtaaaaaatt
     6421 ctagtatttt aaaagtattt tttttaggta ttttgggtgg tttaattgcc ttacttacac
     6481 catgcgtttt tcctatgata cctgttacag tatccttctt caccaaaaga tctgcaaacc
     6541 ataaagaggc tgtaaaaaat ggtactttat atggtgtttt tattttttta atctatatag
     6601 ctgcaagtat tccttttcat ttaattagta atatacagcc agaaatatta aataatattt
     6661 ctaccaatgc atggttgaat attgtattct ttgccatctt tatatttttt gccatttcat
     6721 tttttggttt tttcgaaata tccttaccat ctagtattgc aggtaaagca gatagtaaag
     6781 gaagtttagg cagtataggt ggaattttct ttatggcatt aactttaact attgtttcat
     6841 tttcttgcac tggcccaatt ttaggttcat tattggtagg tacagcaagt ggtggggcaa
     6901 tggagcttac agcaggttta gcaggttttg gtattgcatt ggcattgcct tttgcgttat
     6961 ttgcagtttt tcccaattgg ctaaaatctc ttccaaagag tggtggttgg ttagatactg
     7021 taaaaaaagt tttagctttt gccgagttag cttttgcaat taaattttta tctaatgcag
     7081 atactgttat gcattggggc ttattaaaac gagaagtttt tattggcttg tggatattga
     7141 taagtattgg attagtttta tatttatttg gattcttaag attaccacac gacgacagaa
     7201 cagctaaaat aagtgtgggt cgtaaaatat ttggtttaat agctttggca tttaccattt
     7261 atttaatacc tggtttaact aacaccaagc atgccaacct taaactttta agtggctttc
     7321 ctcctccaaa aaattatagt atttatggca aagaaagtgc acaccataaa ggcttagaag
     7381 ctaacatttc taacaactat ttaaaggcac tacaaatggc taaagaacaa aataagcctt
     7441 tgctaattga ttttactggt tgggcttgtg tgaattgtag aaaaatggaa gaaaatattt
     7501 ggacagttcc agaagttaaa caatttatta cagaccattt tattttactt tctttatatg
     7561 ttgatgataa agaaaaacta cctgttgccg accgatttac ttataaaacc aaaagtggtt
     7621 ttgagaagga tattgttaca gttggagata aatggtcaac ctttcaaacc gaaaattttg
     7681 ggcaaacaac ccaaccttta tatgttatat tagatactga ggaaaaattg ttgaataatc
     7741 ctattggcta tacaccaagc tataatgaat atttaaaatg gttgcaatgc ggtaaaaatg
     7801 cttttcaaaa ataaatattt attactactc tatgtaacta aattataatt taaaaaatgt
     7861 ttttaattta taccaaatga tgaaattgaa tagtgttggc attggtactc gtatcctaaa
     7921 ctttttggta gatattatcc ttatattttt acttgcttat gtagctttta aaattcataa
     7981 atggtatgtg ttttactaca aaataaccta tataaatttt ggatggtttt tctttggtac
     8041 aatgttcttc tactgtttac tattcgaaac attttttgca aaaacaccag gcaaatggtt
     8101 tactcaaact aaagtagtta atctatctgg caataagcct tctttttttg ccatagtttt
     8161 aagaagtttt ataaggctaa ctattattga tgtatttttt attcctttta ttgataaaac
     8221 ccttcacgat tatgtaagta aaacagttgt tgtagaaact tagtcccatt tcgcttcttt
     8281 tataaaaaat acacttatac ttaataacct acattctctt taaatatatt ttaagaaaac
     8341 taattcgtat tcacttctct aacttttaca tattgattct ctaaagtagt attagccaac
     8401 attaaagaaa gacatttttt taaaaaacac tacaatcatc aaataatttt attaattact
     8461 atatttattt gtatattatt gaatatcaat acaaaataat attaataaca atagtcaatg
     8521 gttaattcac taaaaaatta atacccataa aaggtaaaaa tataaactaa ctggataatt
     8581 tatctaaatt caatagtcaa aataatttta aagataattt tatctttatt ttacgttaca
     8641 attgcaaaat tcttttaatc tggttttata aatatttttt gttattaata ataaaagcaa
     8701 aaaatactag atggttgctg tattttaaaa attaaattct gagtcaaaat tttaaactta
     8761 tttattatga aaaagttttt actacttttt accttattag ttacaagttt tagtagcata
     8821 aatgcacaaa ctactgttat tataggaaat caaggatctc aagttgcagg aacaggtcct
     8881 ttgtcttata atagttatgc tagatttcaa acaatatata cagccgccga attatctgct
     8941 gctggtatgg ttgctaatag cgaaattaca gatattgctt tctttataac ttcttcaagt
     9001 aatgtaccta ttaaagacta tactattaga atggggcata ccacagcaac aaatttttcc
     9061 tctcctcttt ctgtaccttt aactacagta agaaaccccg cagattatac accaattcga
     9121 aatccatccg acgcaacatg ggatacttct ttaaatttag ataaccattt tatttggaat
     9181 ggctacgata atattggtat tgatatttgt tctggttcaa gtatattagc ctataacaat
     9241 accgttgctt tatatccata cacaaaagga catagaagtg aaaatacttc ctgccaatac
     9301 ttaccatttg atacttacaa ttataggcct gcaattcaac ttacatttat aacaacatgt
     9361 acaaccccaa cagccttaag cataggtgca ataacagcaa cctcagcatc agcaactttt
     9421 acaagtgctg gtactgcatt tatagtagag tacggagcag caggttttac accaggcact
     9481 gattctacag caggtgtagg aggcacagtt gttacaggtt cagcttctcc aattgcctta
     9541 attggcttaa caggcagtac cacttatgat gtatatgtaa gacagaactg taccaacagt
     9601 ggtgccggat atagtaacaa tagcattaaa gcaacgttta ccacattatg tatgcccggt
     9661 ggagatgaag ttagttatgg caatggttca tgggaaggtt atgtttatac atggacaggc
     9721 tcaccttctt ttaattcgcc caattacaaa ggtaatataa cagaaaacga acaattcgac
     9781 cgagataatg gtgccggagc aatagctggt ttaaccacta atttatgttc tcctattcca
     9841 tctgatggtt atacggtacg atataaaatg acaaaaaatt ttcctgccgg gtattataaa
     9901 tttacagtgg gtggcgatga tggagtacgt ttaagtattg atggtggtag tacatggtta
     9961 gtaaatgttt ggaacgtcca atcttatact atttactcat ctccaactgt attcttaaac
    10021 ggcagtacaa acttggtttt agaatactat gaaaatgccg gagattccag aatttctttt
    10081 tcttatacta ttccttgctc aacaccaact gcattaagtg caggtggcgt taccaataat
    10141 tcagcaaaca taggctttac aagtgctggt actgcattta tagtagagta cggagcagca
    10201 ggttttacac
//
LOCUS       DAOQPK010000063        10173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_3474_length_10173_cov_4.489425, whole genome shotgun sequence.
ACCESSION   DAOQPK010000063 DAOQPK010000000
VERSION     DAOQPK010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10173
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3474_length_10173_cov_4.489425"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PLU36_04535"
     CDS             <1..505
                     /locus_tag="PLU36_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HMZ46049.1"
                     /translation="AIPGINTTAHLLFSMAYPNHANKQFMVFNEIEDAVLNGKVDAGV
                     IIHENRFTYQQKGLKKIIDLGEYWEEKLKIPIPLGGIVAHKRVEESILSTVDELIKKS
                     IEFAFANYPSITDYVKQHAQEMDESIMKQHIDLYVNNYSLDIGNDGKKAVEKLMDIHH
                     LLSTNKS"
     gene            complement(492..1361)
                     /locus_tag="PLU36_04540"
     CDS             complement(492..1361)
                     /locus_tag="PLU36_04540"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HMZ46050.1"
                     /translation="MQTTNEINNKFICFNGLFISNKTAIVDASNRGLKYGDGFFETLR
                     IINNKIVLKEFHFNRLFDALQSLHFEQPNFLTSDFLEEKILNLIQKNNFTTAAKVRIN
                     IFRGNGNLFEIENNYPNYIIEAEPLPNQFYEWDKTGLKVDFFIDAKKVCDNFSHLKTN
                     NFLPYSMAAFWAKKNNLHSAIILNQHNCVADSAIANVWVIKNGEIQTPALTQGCIDGV
                     MRKYLIKCFKEEGLPFKETALTIEDIVQAQEVFLTNVIKGISWVKFCGKTEYTNTTTQ
                     FIYSKFVHTLNSY"
     gene            1478..2566
                     /locus_tag="PLU36_04545"
     CDS             1478..2566
                     /locus_tag="PLU36_04545"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HMZ46051.1"
                     /translation="MENSQHNKINSYIFLSVIIVFGLFLLFSLSELFTAFLGALMFYI
                     LSKPLVEWLIKNKKWNKSLAVVLVIIISFFIILLPILLIGSLLYKEVSIIASSPEVII
                     KPINEFDALFYQKFNIRLISVKNLGSIQSYATSILSSALNIGLNFFTSITMMYFFLYF
                     LLTNINRLEAAIILYLPFKKNNIKLFGKELVAQTVSNAIGVPLIALIHGLLAYIAYKI
                     AGLEQAAFWAIITAFASVIPVIGTALIWLPVSLLLFIKGQTGYGVFIIIWGIVIIGLS
                     DNLFRFLLAKKMADVHPIVTVLGLIIGLKYFGFTGLIFGPLMISYFIILLKIYYNQYQ
                     KPLKQKRKHNLTMPTYFNIPFITNKKKH"
     gene            complement(2563..4074)
                     /gene="hutH"
                     /locus_tag="PLU36_04550"
     CDS             complement(2563..4074)
                     /gene="hutH"
                     /locus_tag="PLU36_04550"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HMZ46052.1"
                     /translation="MATKNVLNIDNSWLSFDEVKQFITQNSTIAISNQSIQLVLKCRN
                     FLDEKMANNNALFYGINTGFGFLQNVKIDANQIEQLQYNLLMSHACGMGNEVPTHIVR
                     LMLLLKIKSLSYGNSGVQLNTINALATLCNNNILPIVFMQGSLGASGDLAPLSHLCLP
                     LIGMGEVQIENEKFATKDILNKLGFNPVALKSKEGLALINGTQFMSAYGMYCLYKAER
                     LLRLADIIAAISFDAFNCVKEPLHPAIHHVRKHKGQVATAASLRKLLEGSEISNQPKK
                     QVQDAYSFRCIPQVHGASKDVFENVLEVFLTEINSVTDNPNVFPDEDLIVSGGNFHGQ
                     PLALRLDFLSIAMSEIGNISERRTYQLISGLRELPAFLVNSPGLNSGFMIPQYTAAGI
                     VSENKQLCTPASVDSISSSNNQEDHVSMGANAATKCMRVVDNVERVLAIELLTAAQAL
                     DFRRPAKTSPILEKFVAAFREKVSFNEQDRILHTDMIAAVEFLRTVEITNEMY"
     gene            4183..4256
                     /locus_tag="PLU36_04555"
     tRNA            4183..4256
                     /locus_tag="PLU36_04555"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4217..4219,aa:Arg,seq:tct)
     gene            complement(4325..5572)
                     /locus_tag="PLU36_04560"
     CDS             complement(4325..5572)
                     /locus_tag="PLU36_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ46053.1"
                     /translation="MNPQKNYSVLSLPVIVAALGYFVDIYDLLLFTIVREPSLQGIGI
                     NLADKAAVLNASTKIINWQMLGLLIGGIVWGTIGDKKGRLSVLFGSIAMYSVANFLTG
                     YVQTTGQYSFARFVAGIGLAGELGAGITLVSELLPKNKRGIGTSLVAGIGLFGAVFAY
                     YTFYITNQDWRLCYKIGGGLGFILLLLRVSVAESGMFKEVKNKAIQRGNFFMFFTNAK
                     RFKKYAFAILIGLPTWYVIGVLVNYSNRFATEFYGTNAIISGKAIMYAYIAIAFGDIL
                     IGFVSQYFKSRKKALLLFYILCIIGMILFYSPLNNSDFNMYAICAFLGFSTGFWAIFV
                     TIGAEQFGTNLRATAATTIPNMVRGALPLINLLFLDVFQKSWNLSLIVSGIITAVIVM
                     SIALIAFYYTEETFHKDLNYIEE"
     gene            complement(5703..6638)
                     /locus_tag="PLU36_04565"
     CDS             complement(5703..6638)
                     /locus_tag="PLU36_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HMZ46054.1"
                     /translation="MKQSLFLLILCICISSISFAQTDTITIKVQNQYEAEVEAVNIKV
                     LSTGKSFVANEKGFATINASQIDSIELTKEGYTSTVVTVNQLKNKKVVIEKKFTWKDL
                     LTPMFYIINGGLWLLLFIVFAETGLFVGFFLPGDSLLFVAGIFSNMLAAYFFDTGSDF
                     VNLLMIISLVSLAGILGNTTGYWFGRKIGPAMYHWKDNFLFKQKYLQQAHEFYEKYGG
                     GAIVFARFLPVVRTFAPIIAGIVQMDKKKFGLFNIIGSFAWAGSMIAAGHYLDKLFID
                     KFDIDLKKHLEIIIIIIVIVSNTPVIIKLLGKKKH"
     gene            complement(6743..8014)
                     /locus_tag="PLU36_04570"
     CDS             complement(6743..8014)
                     /locus_tag="PLU36_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HMZ46055.1"
                     /translation="MSTPSTTAPKKSKLTLWIIVAMILGVLVGYLVFNNTTETFRKGF
                     SGNIKLLSTVLIRLVQMIIAPLVFTTLVVGISKLGDLKTVGRVGGKAMIWFITASLIS
                     LLIGLVLVNYFKPGQYIQLGLQDSEGLKDLMTKQTSFSLQNFVEHVIPKSFIEAMATN
                     EILQLVIFSIFFGVASASIGEYAKPVIHVLEVASHIILKIVNYVMNFAPLGVFGAIAA
                     VVASKGLVIFDFYIKYFLFFLIGIAALWIVLLTVGFIILKGRLKILLKRIGNPLLIAF
                     STTSSEAVFPKLTEELERFGCKNKIVSFVLPLGYSFNLDGSMMYMTFASIAIAQVYNV
                     PIATDLPQQLTMLLVLMLTSKGIAGVPRASLVVVLATCAMFNIPPIGVALILPIDHFC
                     DMFRTATNVIGNALATSVVSKWEGELENSNT"
     gene            8173..9651
                     /locus_tag="PLU36_04575"
     CDS             8173..9651
                     /locus_tag="PLU36_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HMZ46056.1"
                     /translation="MGLFTKKSIDILLSEANETGENSLKRTLGGWGLIALGIGAIIGA
                     GLFVRTAAAIAQNAGPSVTIGFLVAALGCTFAGLCYAELSSSIPISGSAYTYTYATMG
                     EFLAWIIGWDLVLEYAVGAATVGIAWSEYLNNLLVEVFHLSPIPYEWCHSPFQTSVDG
                     VNGIINLPALGIVVLLSLLLIRGIKESTLVNNFIVILKVAIVILIIALGWSYINPAYH
                     TPYIPEATKYTDHHGTTYNFGGVMGILGAAGTVFFAFIGFDAVSTAAQETKNPKKDMP
                     FGILGSLIICTLLYVLFSHVLSGLAPVDFFRDPSKGGEASVVAAIKEAMHGYGWLSKS
                     ITVAILAGFSSVILVMLLGQSRVFYSMSKDGLLPKAFSDVHPKYKTPYKGNLVILILV
                     ALFAAFVPGDVVGHMTSIGTLFAFMLVCIAVIILRKKEPNLPRQFKTPLVPLIPILGV
                     LSCGAMIFGLGWENWARLIGWLALGFIIYFGYGKRHSKLRNK"
     gene            complement(9736..>10173)
                     /locus_tag="PLU36_04580"
     CDS             complement(9736..>10173)
                     /locus_tag="PLU36_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HMZ46057.1"
                     /translation="DPYVIEYNCRMGDPETEVILPRLETDLVSLFAAMGNNTLADANI
                     EFFEESFATIMAVSGGYPSTYKTNYIIHNLNNLKTEKDILIFHAGTKQNENGEIVTNG
                     GRVLAVTAKGSSIKNAVEKSIHVLNSISFEGMYFRRDIGYEFK"
BASE COUNT         3522 a         1670 c         1439 g         3542 t
ORIGIN      
        1 tgcaattcca ggaataaata caacagcaca tttattattt tccatggctt accctaacca
       61 tgctaataaa caatttatgg tgtttaatga aattgaagat gctgtgttaa atgggaaagt
      121 ggatgctggt gttattattc atgaaaacag atttacctat caacaaaaag gattaaaaaa
      181 aataattgac ttaggcgaat actgggaaga aaaattaaaa attccaattc cgttaggtgg
      241 tattgttgca cataaaagag tagaagaaag cattctttct acagtagatg aactaattaa
      301 aaaaagtatt gaatttgctt ttgcaaacta tccttctatt acagattatg taaagcaaca
      361 tgctcaggaa atggatgaat ccattatgaa gcaacatata gacttgtatg taaataacta
      421 ttctttagat attggaaatg atggtaaaaa agcagttgaa aaattaatgg atatccacca
      481 tttgctatct actaataaga gttgagtgta tgtacaaatt ttgaataaat aaattgagtt
      541 gtggtattgg tatattcggt tttaccacaa aacttaaccc aactaatgcc tttaataaca
      601 ttagttaaaa atacttcttg tgcttgtact atgtcctcaa tggttaatgc agtttcttta
      661 aaaggtaatc cttcttcttt aaaacattta attaaatatt ttctcattac accatcaata
      721 catccttgag ttaatgcagg agtttgtatt tctccatttt taattaccca aacattggca
      781 attgcagaat ctgcaacaca gttatgttga tttaagatga ttgctgaatg tagattattt
      841 ttttttgccc aaaatgctgc cattgaataa ggtaaaaaat tattggtttt aagatgagaa
      901 aaattatcgc aaactttttt tgcatctata aaaaaatcaa ctttcaaccc agttttatcc
      961 cattcataaa actggtttgg tagtggttct gcctctatta tataattggg ataattattt
     1021 tctatctcaa acaaattccc attccctcta aaaatattta ttctaacttt tgctgctgta
     1081 gtaaaattat ttttttgaat aaggtttaaa attttttctt ctaaaaaatc agatgtcaaa
     1141 aaattaggtt gctcaaaatg aagagactgc aatgcatcaa ataacctatt aaaatgaaat
     1201 tcttttagta caattttgtt atttattatc ctcaaagttt caaaaaaacc atctccatat
     1261 ttcaatcccc tgttagatgc atctacaatg gcagttttat tactaataaa caatccatta
     1321 aaacaaatga atttattatt tatttcatta gttgtttgca tacatttcaa taaagtaaaa
     1381 aatactactt ttaagcagaa attattttac aatttaagct ttatattttt tacaaaattt
     1441 tgtttttgta aatatagcta tgcaaaaaat agagttcatg gaaaacagtc aacataataa
     1501 aataaacagc tacatttttt tatcggtaat tatagttttt ggactttttc tgctttttag
     1561 cttatccgaa ttgtttacag catttttagg tgcattaatg ttttatattt taagtaaacc
     1621 attggtagaa tggctcatca aaaataaaaa atggaataaa agtttggctg ttgttttggt
     1681 aattatcatc tcatttttta tcatattatt accaatttta ttgataggaa gtttactgta
     1741 taaagaagta agcattattg caagcagccc agaagtaatt ataaaaccaa ttaatgagtt
     1801 tgatgcctta ttctatcaaa aatttaatat ccgtttaatt tctgtaaaaa atttaggaag
     1861 tattcaaagt tacgccacca gtattttatc atctgcctta aatattggct tgaatttttt
     1921 tacaagtatt accatgatgt atttcttctt gtatttttta ttaaccaata taaacagatt
     1981 agaagctgca attattttat accttccttt caaaaaaaat aatattaaac tttttggaaa
     2041 agaattggta gcacaaactg ttagtaatgc tattggtgtt cctttaattg cattaataca
     2101 tgggctatta gcctatatag cctacaaaat tgcaggactt gaacaagcag cattttgggc
     2161 tattattact gcatttgcat ctgtaattcc tgttattggc acagctctta tttggttgcc
     2221 agttagttta ttattattta taaaagggca aaccggttat ggtgttttta taattatttg
     2281 gggtatagtt attataggat taagtgataa tttatttcgt tttttattgg caaaaaaaat
     2341 ggcagatgtg cacccaatag ttacagtatt agggttaata attgggctga aatattttgg
     2401 atttacagga ttaatttttg ggccattaat gatttcttat tttatcattc ttttaaaaat
     2461 ttattataat caataccaaa aaccattaaa acaaaagcga aaacataatc taacaatgcc
     2521 tacttatttc aacattccat ttattacaaa taaaaaaaag cattagtaca tttcattggt
     2581 tatttctact gttcttaaaa attcaacagc tgcaatcata tctgtatgta aaattctatc
     2641 ttgttcatta aatgaaacct tttctctaaa tgcagcaaca aatttttcaa gaattggcga
     2701 agttttagca ggccttctaa aatctaatgc ttgagctgca gttaaaagtt ctattgctaa
     2761 aactctttct acattatcta caaccctcat acatttggta gctgcattag cccccatgct
     2821 tacatgatct tcctgattat tagaagatga aatactatct acactagcag gtgtgcacaa
     2881 ttgtttattt tcactaacaa tacctgcagc tgtatattgt ggtatcataa acccagaatt
     2941 taaacctgga gagtttacta aaaacgcagg taactctctt aacccagata ttaattgata
     3001 cgttcttctt tcgctaatat taccaatttc gctcatggct atactcaaaa aatctaatcg
     3061 tagagctaaa ggctggccat gaaagttgcc accactaact attaaatctt catcaggaaa
     3121 aacgtttgga ttatccgtta cagaattaat ttcggttaaa aatacttcta acacattttc
     3181 aaaaacatct ttacttgcac cgtgtacctg aggaatgcat ctaaagctat aagcatcttg
     3241 tacttgcttt ttaggttgat tacttatttc acttccttcc agcaattttc ttaaagaagc
     3301 agcagtagct acttgccctt tatgttttct tacatgatgt attgctggat gtaaaggctc
     3361 ttttacacag ttaaaagcat caaaactaat agctgctata atatctgcca accttaataa
     3421 tctttctgct ttatataaac aatacatacc ataagcactc ataaattgag taccatttat
     3481 taacgctaaa ccctctttac tctttaatgc tactgggtta aaaccaagtt tatttaaaat
     3541 atcttttgta gcaaattttt cattttctat ttgcacctct cccatgccaa ttaatggtaa
     3601 acataaatga gatagtggtg ctaaatctcc acttgcacct aaggaacctt gcataaaaac
     3661 aataggtaaa atattattat tgcagagtgt tgctagtgcg ttaatagtgt taagttgtac
     3721 tccactatta ccataactta agctttttat ttttaataat agcattaacc taacaatgtg
     3781 ggtaggcact tcattaccca taccacaagc atggctcatt aataaattat attgtaattg
     3841 ctcaatttga ttggcatcta tttttacatt ctgtaaaaaa ccaaaaccgg tgttaatccc
     3901 ataaaacaaa gcattattat tggccatttt ttcatctaaa aagttacgac attttaacac
     3961 caattgtata gattgatttg aaattgcaat tgtgctattt tgagttataa actgttttac
     4021 ctcatcaaaa gagagccatg agttatcaat atttaaaaca tttttagtag ccatatattt
     4081 aaattgtttt tataggtgca atgtagcttt ttatatagat attttttaaa taaagttggc
     4141 ataaacataa ttttcctttt attttgcaac tcctttaaaa aaggtccggt agctcagctg
     4201 aatagagcaa cagccttcta agctgtgggt cattggtttg aatccaatcc ggatcacata
     4261 aaaagccttg ataattcagg gcttttttgt tttgtatagt aaccatttta agcactcttc
     4321 ttttttactc ttcaatataa ttcaaatctt tatgaaaagt ttcttccgta tagtaaaatg
     4381 ctatgagtgc aatactcatt acaattacag cagtaataat gccactcaca attaaactca
     4441 aattccagct tttttgaaaa acatctaaga aaagtaaatt aattaaaggt aatgcaccac
     4501 gaaccatatt aggaattgtg gtggctgctg tagctcttaa attagtacca aactgttcgg
     4561 cacctattgt tacaaaaatt gcccaaaagc cagtactaaa cccaagaaag gcacatattg
     4621 catacatatt aaaatcagaa ttgtttagtg ggctataaaa caatatcatt cctataatac
     4681 aaagtatgta aaaaagtagt aatgcctttt ttctactttt aaaatactga ctaacaaagc
     4741 caattaaaat atcgccaaag gcaatagcaa tataagcata cataattgcc ttaccactaa
     4801 tgatagcatt ggttccataa aattcagttg caaaacggtt actataattt accaaaacgc
     4861 caattacata ccaggtaggc aaaccaatta aaatagcaaa ggcatatttt ttaaaccttt
     4921 tggcattggt aaaaaacata aaaaaattac cacgttgaat ggctttattt tttacttctt
     4981 taaacattcc actctctgca acagaaactc ttaaaagcaa taaaataaaa cctaatcctc
     5041 cgccaatttt gtaacacaat ctccaatctt gattagttat ataaaaagta taataagcaa
     5101 atacagcacc aaacaaacca attcctgcaa ctaaagatgt accaatgcct cttttgtttt
     5161 taggaagcaa ttcacttact aaagtaattc ctgcacctaa ttcgccagca aggccaatgc
     5221 ctgcaacaaa ccttgcaaaa ctatactgcc ctgttgtttg aacataacca gttaaaaaat
     5281 tagcaacaga atacattgca atggatccaa ataatacact tagccttcct tttttatctc
     5341 ctattgttcc ccaaacaata cctccaataa gtaagcccaa catttgccag ttaataattt
     5401 tagtacttgc attgagtact gctgctttat ctgcaaggtt tataccaata ccttgtaaac
     5461 tgggttctct tacaatggta aaaagtaata agtcgtaaat atctacaaag taacctaatg
     5521 cagcaacaat tacaggaagt gaaagaacac tatagttttt ttgtggattc atgtagtgat
     5581 tagttgtttg atgactattt tgaaaagtat caaaatttga attaaaaaaa atgcattaac
     5641 tgaaataaaa ttacagtttg caacaactca aacatcaaac aaataaaaaa cagagaagat
     5701 aattaatgtt ttttctttcc taaaagcttt ataattacag gagtattaga aacaataaca
     5761 ataatgataa tgatgatttc caaatgtttt ttcaaatcaa tatcaaactt atcaataaac
     5821 aatttatcta agtaatggcc agcagcaatc atactacctg cccaggcaaa tgagccaata
     5881 atattaaaca atccaaattt ctttttatcc atttgtacta tgccggcaat aattggtgca
     5941 aaagttctta caacaggtaa aaacctagca aatacaattg ccccaccacc atatttttcg
     6001 taaaactcgt gtgcttgctg caaatatttt tgtttaaata agaagttatc tttccaatga
     6061 tacattgcag gcccaatttt tcttccaaac caataacctg tagtattacc taaaatacca
     6121 gctaatgaaa ccagcgaaat aatcattaaa agatttacaa aatcgctacc agtatcaaaa
     6181 aaataagcag ctaacatatt actgaatatt cctgcaacaa ataataaact atcccctggt
     6241 agaaaaaatc caacaaataa ccctgtttcg gcaaatacaa taaataagag taaccataac
     6301 cctccattaa taatataaaa cattggggtt agcaagtctt tccaagtgaa ttttttttca
     6361 attacaacct ttttgttttt aagttgatta accgtaacaa ctgtactggt ataaccttct
     6421 ttagttaatt ctattgaatc tatttgagat gcattgatgg ttgcaaaacc tttttcatta
     6481 gctacaaagc tttttcctgt acttagtact tttatattta cagcttctac ttctgcctcg
     6541 tattggtttt gaactttaat tgtaattgta tctgtttgtg caaaagaaat gctagaaata
     6601 caaatgcata gtatcaataa aaatagagat tgtttcataa aaattaagtg cagttctgta
     6661 ttaataagga ctagtgttgg taattattat ttggcaatga tataataaaa aaatgtagta
     6721 gggttgttaa aaaaatagta aatcaggtgt tactattttc caattcacct tcccatttac
     6781 ttacaacgct tgttgcaagg gcattaccaa ttacattggt tgctgttcta aacatatcgc
     6841 aaaaatgatc tattggtaat attaaagcta caccaattgg tggaatatta aacatagcac
     6901 aagtagccaa aaccactact aaacttgccc ttggtacacc tgcaatccct ttgcttgtaa
     6961 gcattaaaac aagtaacata gtaagttgtt gtggaagatc tgttgctatt ggtacattat
     7021 aaacctgagc aatagcaata cttgcaaaag tcatgtacat catactacca tctaagttaa
     7081 atgaataacc caatggaagt acaaacgaaa caattttatt tttacaacca aatctttcta
     7141 actcttctgt aagttttgga aatacagctt cgctacttgt tgtactaaaa gcaattaata
     7201 atggattgcc aattctttta agtaaaattt ttaaccttcc ttttaaaata ataaacccaa
     7261 cagttaacaa tactatccaa agtgctgcaa tgccaattaa aaagaataaa aaatatttaa
     7321 tataaaaatc aaaaattact aatcctttag aagcaactac agcagctatt gcaccaaaaa
     7381 cacctaatgg tgcaaaattc attacatagt taactatttt aagaattata tgagaagcaa
     7441 cttcaagaac atgaattaca ggttttgcat actcaccaat tgatgctgat gctacaccaa
     7501 aaaagataga aaaaattacc aactgtaaaa tttcattagt agccattgcc tctataaaac
     7561 ttttgggtat tacatgctca acaaaatttt gaagtgaaaa agatgtttgc ttagtcatta
     7621 aatctttcaa cccttcgctg tcttgtaatc ctaattgaat gtattggcca ggtttaaaat
     7681 aatttacaag tactaagcct attaataaag aaattaaaga agcagtaata aaccatatca
     7741 tagctttacc tcctactcta ccaacagtct ttaagtcgcc aagtttagaa ataccaacta
     7801 ctaaggtggt aaaaactaat ggagcaataa tcatttgtac caaacgtatt aacacagtgc
     7861 ttaacaattt aatgttacct gaaaatcctt ttctaaaagt ttcggtagta ttattaaaca
     7921 ctaaataacc taccaaaacg ccaagaatca tggcaacaat tatccaaagt gttagtttag
     7981 attttttagg ggcagtagta cttggagtac tcatgtatgt tttattttct aatttaaaaa
     8041 ggcaatatat agtatttagc tcaatatcta cactaatcaa ataataacca ctaataaaag
     8101 ttttataata attatttgag attatcccta tttttaggca atcaaactga aactattaac
     8161 caaaacaatt ttatgggatt atttacaaag aagtctattg atattttgct atctgaagca
     8221 aatgaaaccg gcgaaaactc acttaaaaga accttaggag gatggggttt aattgcttta
     8281 ggaattggtg caattatagg agctggttta tttgttcgta cagctgcagc cattgctcaa
     8341 aatgctgggc cttcagtaac tataggtttt ttagtagcgg ccttaggatg tacttttgca
     8401 ggtttgtgtt atgctgagct ttcttcctct attcctatat ctggtagtgc atacacctat
     8461 acttatgcta ccatgggtga atttttagca tggataattg gatgggattt ggtacttgag
     8521 tatgcagttg gtgctgctac tgttggtatt gcatggagtg aatatttgaa caatttatta
     8581 gtagaagttt ttcatttaag ccctatacct tatgaatggt gtcattcacc atttcaaacc
     8641 tctgtagatg gggttaatgg cattattaat ttacctgctt taggtattgt tgttttactt
     8701 agtttattat taattagagg tattaaagaa tctacactag taaacaactt tatagttatt
     8761 ttaaaagttg ctattgttat tttaataatt gctttaggtt ggagttatat taaccctgct
     8821 tatcacacac cttacattcc tgaagccaca aaatatacag atcatcatgg tactacttac
     8881 aattttggag gcgttatggg cattttagga gcagctggta cagtattttt tgcctttatt
     8941 ggatttgatg ctgtaagtac tgctgcacaa gaaactaaga atcccaaaaa agatatgcca
     9001 tttggcattt taggttcact aattatttgt acccttttat atgtattatt ctctcatgtt
     9061 ttatctggtt tagcacctgt agattttttc agagatccta gtaaaggtgg agaagcatca
     9121 gttgttgctg ctattaaaga agcaatgcat ggttatggtt ggttaagcaa atctatcact
     9181 gtagctattc ttgctggttt ttcttctgtt attcttgtaa tgctattggg acaaagccgt
     9241 gtattttact ccatgagtaa agatggttta ttaccaaaag cattttctga tgttcatcca
     9301 aaatataaaa caccgtataa aggcaacttg gtaattctta ttttagttgc attatttgct
     9361 gcctttgtac ctggagatgt ggtaggccac atgacaagca ttggtacctt atttgcattt
     9421 atgttggttt gtattgctgt aataatttta agaaaaaaag aaccaaactt acctcgccaa
     9481 tttaaaacac cattagtgcc attaatacca attttaggtg tattatcttg tggtgctatg
     9541 atttttggat taggttggga gaactgggca agattaattg gctggcttgc tcttggtttt
     9601 attatttatt ttggttatgg aaaacgccac agcaaattaa gaaataaata aatattccgt
     9661 tttattaaca acaataaata aaggctgcaa cttaaatatt gcagccttta aaattttcaa
     9721 tactagtagc attatttact taaactcgta gccaatatct cttctaaaat acataccttc
     9781 aaaagatatt gaatttaaaa catggataga tttttctaca gcatttttta tactactccc
     9841 ttttgcagtt acagcaagca ctcttcctcc attagttaca atctctccat tttcattttg
     9901 tttagtgcct gcatgaaaaa ttaaaatatc cttctcagtt tttaaattat ttaagttatg
     9961 aatgatgtaa tttgttttat aggtagaagg atagccacca ctaacagcca taattgttgc
    10021 aaagctttcc tcaaaaaatt caatattggc atctgccaaa gtattgttac ccattgctgc
    10081 aaaaaggctt actaaatctg tttccaacct tggtaatatt acttcagttt cagggtcgcc
    10141 catacggcaa ttatattcta ttacataagg gtc
//
LOCUS       DAOQPK010000064        10061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_3537_length_10061_cov_4.151409, whole genome shotgun sequence.
ACCESSION   DAOQPK010000064 DAOQPK010000000
VERSION     DAOQPK010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 10061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10061
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3537_length_10061_cov_4.151409"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PLU36_04585"
     CDS             <1..337
                     /locus_tag="PLU36_04585"
                     /inference="COORDINATES: protein motif:HMM:NF014786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HMZ46058.1"
                     /translation="ATDANRLVKVVRSDVKPGVKHSFILPKKALNLLKSSLMGASVPV
                     KIDYNRSNAFFTFGDTKLVCRFIDEKYPDYNGVIPKENPNILVMNREDLYNSVKRISI
                     YSNKSTFLF"
     gene            453..680
                     /locus_tag="PLU36_04590"
     CDS             453..680
                     /locus_tag="PLU36_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46059.1"
                     /translation="MTIENFKSLPIEKKLIELKHSAELLGSYERNSENGGPKTPGDIY
                     ALYDFWVFLSDDEKMLIPTRRNPFPEVEEEE"
     gene            913..1749
                     /locus_tag="PLU36_04595"
     CDS             913..1749
                     /locus_tag="PLU36_04595"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HMZ46060.1"
                     /translation="MFKLFSTGYLLLSTSLIFTLSSCGNSKNEKVSNTDSLNNVQAKP
                     TPGTPIKYDSSKRYIFLTWDDSPQPPGTTNCLKIFKEEGVKSTFFAVGFNQVGPHKKR
                     IIDSIRNSYPNVLLANHSFSHGFNDKYTKFYNTPDSAYNDFMRNERDLNIGVKIIRLP
                     GNNTWAFNGKIHGQKADNAIVKKLDANGYKIIGWDLEWMQQPKAKAPKQSVEEMIKSI
                     NQKFDEGMTNEANMLVILSHDRLFEKQQYADSLRKFIQVLKSDPRNVFETIDHYPLVQ
                     NK"
     gene            1762..2778
                     /locus_tag="PLU36_04600"
     CDS             1762..2778
                     /locus_tag="PLU36_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HMZ46061.1"
                     /translation="MIDFRSDTVTKPTKQMLEAMLQAKVGDDVFGEDPTVNELEEYAA
                     NLFGIQAALFCPSGTMTNQIAIKVHTQPGDEIICDNLSHIYQYEGGGIAVNSACSVKL
                     INGNYGKINAKQVAENINNINDVHKPISKLLSLENTSNRGGGSCYHFDDFIAIKEVCT
                     QNNLMLHLDGARLFNAIIYKNESAKQYGKVFNSISICLSKGLGAPIGSLLLGDKNFIL
                     KARRVRKVFGGGMRQAGIIAAAGLYALQNNIQRLSTDHHHAQLIATALRQKSFVTNIL
                     PVETNIIIFEVAEKYTAKELIATLYKNNILAFAIAENQIRLVLHLGITTEMVEETIQL
                     IQKL"
     gene            2795..4000
                     /gene="lysA"
                     /locus_tag="PLU36_04605"
     CDS             2795..4000
                     /gene="lysA"
                     /locus_tag="PLU36_04605"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HMZ46062.1"
                     /translation="MQQPTNEQFLEIAQQFGTPVYVYDANKIVSQYQKFKSAFNSPTV
                     KIFYACKALTNINILKLLKLQGANIDCSSINEVKLAIHAGFEPQQILYTSNGIGFDEI
                     EEAKNLGIHINIDSISNLKKFGQKFNNSYPVGVRLRPNIMAGGNIKIATGHDKSKFGI
                     PITQIAEVVDIMQQYNLLINGVHIHTGSDIKEADVFLKGIEVLFDVIHLFPNLMYVDL
                     GGGFKVPFEDTEEETDMITLANKILEKFNQHLHQNKLQIWFEPGKYLVSQAGYFLTKI
                     NVLKKTNNIEFASINSGFNHLLRPMFYDAYHKIVNISNPNGTIKEYDIVGNICETDTF
                     AANRNINEINEGDLLVFYNAGAYGFEMSSNYNSRYKPAEVLIHNNQISLIRKADRFED
                     LLKNQIVVL"
     gene            4135..5760
                     /locus_tag="PLU36_04610"
     CDS             4135..5760
                     /locus_tag="PLU36_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ46063.1"
                     /translation="MLSAKNITLAYGKRVLFDEVNINFTKGNCYGVIGANGAGKSTFL
                     KILSGEIEPNKGTVEIGKGERMSFLKQNQFEFDEETVLNTVLMGHKQMMELMQKKDAI
                     YANPDATEEDYMKAGELEAEFGEMGGYTAESDAAALLGSLGVKEEFHNSLMKDIPGNL
                     KVRVLLAQALFGNPDILLLDEPTNGLDIETIGWLENFLADYENIVLVVSHDRHFLDAV
                     CTHVADVDRQKIKTFTGNYTFWYESSQLMSRQINDKNKKVEEKRQALIDFIARFSANA
                     SKSKQATSRKKALEKLTIEEIEPSNRKYPGIIFQPLREVGNQILNVENLSKTIDGRTL
                     FSKATFSVNKNDKIAFLSKDPLAVTNFFEIINDNIKSNEGKFEWGTTITKAYLPMENN
                     EFFTEGLSLMEWLRQFVPAHVTDADEPFLRGFLGKMLFSGDDIAKKTNVLSGGEKVRC
                     MISRMMLQNPNLVVLDQPTNHLDLESIQSFNEGCQTFPGIVLLTSHDHTFIQTVANRI
                     IELTPKGIIDRLMTFDEYMDDKRVQELRIEMYS"
     gene            complement(5953..8319)
                     /locus_tag="PLU36_04615"
     CDS             complement(5953..8319)
                     /locus_tag="PLU36_04615"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HMZ46064.1"
                     /translation="MTTNMLFLIVPILGIVGLLYTFIKFNWVAKQDAGNERMKEISNY
                     IAEGAMAFLKAEWKILTYFVIIVALLLGFMATKNPHSHWSIAIAFILGAICSAVAGYI
                     GMKVATKANVRTAQAARTSLSKALSVSFTGGAVMGVGVAGLAVLGLGGVFIILKGMFA
                     PEAVNSLEMLKTIEVLTGFSLGAESIALFARVGGGIYTKAADVGADLVGKVEAGIPED
                     DPRNPATIADNVGDNVGDVAGMGADLFGSYVATVLATMVLGQETSSIDNFGGYAPILL
                     PMLIAGIGILFSIVGTWFVRVSDAAGINTANVQKALNMGNWGSIILTALACAGLVYYI
                     LPETMSLRGFEFTKWGVLGAIGVGLVVGALMSIITEYYTAMGKRPVMSIIRQSATGHA
                     TNVIGGLAIGMESTFLPILVLAGGIWGSYECAGLYGVAIAAAGMMATTAMQLAIDAFG
                     PIADNAGGIAEMSELPSDVREKTDVLDAVGNTTAATGKGFAIASAALTSLALFAAFVG
                     VSKITGIDIYKAKVLATLFVGAMIPFIFSSLAIRAVGEAAMAMVEEVRRQFKTIPGIM
                     EGKGKPEYDKCVAISTEASIKKMMLPGAIAILSPIIIGFTLGSEALGGFLAGATVSGV
                     LLGMFQNNAGGAWDNAKKSFEKGVNINGEMFYKKSEPHKASVTGDTVGDPFKDTSGPS
                     MNILIKLMSIVSLVIAPTLAQLHSNETVTVDKKIEITTTTSISENNYTNFVGALQKDG
                     LLQSKASLSYENGIITINGKVVDAEIAKKYIALLKDNKSDIKMNFNIE"
     gene            complement(8543..>10061)
                     /locus_tag="PLU36_04620"
     CDS             complement(8543..>10061)
                     /locus_tag="PLU36_04620"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ46065.1"
                     /translation="RYSLKNLKMGQYKAELKAKNWFLRGYLISENSGDSYANTITAVQ
                     MNRMWKADATWFSQYMTAYTQTMLGTNGNATLAHAQGRAAADQGRYMPGSTGFMNAFE
                     QVTGTSITNGGGLFADKSKLYHFEGQYNLTDKIKVAEVLVGASYRVFNLNSGGTIFAD
                     HTPNFKNGNINISEVGGYVQVAKRVNDFLKLTGSIRYDKNQNFDGRFTPRATALFKVA
                     KDNNIRVSFQTAYRFPSTQDQWINLNSPSAKLIGALPIFNDVYGFSTNPIYTAESVGL
                     FRAKFAATGAIDPTVLVKGTFQAVKPETVKSYEIGYRGVIKNKLMIDAYFYFSEYNNF
                     LGRVAVARGQNGTQMELLSPSSSNNYSFVTNTASNVKAIGWGISADYMLPKGFVFNAN
                     VSGDQLNDVPSTVFTQFNTPKMRFNIGLSNTNVYKNWGFGIVHRWQDKVNWEGTFGTG
                     VIPSYGTWDAMVSYKTANKGLFKIGALNFWNKYYRSAFGNPQVGGVYYISYGYNL"
BASE COUNT         3618 a         1648 c         1540 g         3255 t
ORIGIN      
        1 ggcaaccgat gccaaccgtt tggttaaagt ggtaagaagc gatgtaaaac ctggtgttaa
       61 acacagtttt atccttccta aaaaagcgct taacttactt aaaagcagtt taatgggagc
      121 ttcagtacca gttaaaatag attacaaccg ctcaaatgca ttctttacat ttggtgatac
      181 caaattggtt tgccgtttta tcgacgaaaa atatcctgat tataatggcg taatcccaaa
      241 agaaaatccg aatatcttag ttatgaatcg cgaagattta tacaattcag ttaagcgtat
      301 ctccatctat tcgaataaat caaccttttt attttagttg atgaaagagt tttttaagat
      361 aataatagtt attaagaaaa gactttgtat aattaagtag actcttgtca gaataaacaa
      421 aacttaaaat tattaaccta tatacttagt ttatgacaat tgaaaatttt aaatcattac
      481 ccatagaaaa aaaattaatt gagttaaaac actccgccga attgttagga tcttacgaaa
      541 gaaattctga aaatggtgga cctaaaacac caggtgatat ttatgcttta tacgattttt
      601 gggtattctt aagcgatgat gaaaaaatgt tgattcctac tagaagaaac ccttttcctg
      661 aagtagaaga agaagaataa ttaatatttc tatcatttaa ttatagtggg tataacttgc
      721 aagttgctgg ttataccttt tttatgtgcc caaaaaaatt tcatcatttc ttcttttaat
      781 ttcctatcaa tttataatta ataatcttac tcatagtata atttacatat ttacaaaact
      841 tattcactag gtattaataa cattaatttt tgctacttaa tatgattttt atatttgaac
      901 aaaataaatt ctatgttcaa attattttct acaggttatc tgctactttc tacttcgtta
      961 atttttacgt taagtagttg tggaaattca aaaaatgaaa aagtatccaa tacagattct
     1021 ttaaacaatg tacaagccaa acctacacct ggaactccaa taaaatacga tagcagtaaa
     1081 cgctacattt ttttaacttg ggatgattct cctcaaccac caggtacaac caattgttta
     1141 aaaatattta aggaagaagg tgtgaaatct actttttttg cagtaggctt taaccaagtt
     1201 ggccctcaca aaaaacgtat catagattct atacgaaaca gctacccaaa tgtactgtta
     1261 gccaatcata gtttctctca tggctttaat gataagtaca ccaaatttta taacactcca
     1321 gatagtgctt acaatgattt tatgcgtaat gaaagagatt taaacattgg agtaaaaata
     1381 ataagactgc caggcaataa cacatgggca tttaatggca aaatacatgg gcaaaaagca
     1441 gacaatgcaa tagtaaaaaa attagatgct aatggttata aaattatagg ctgggattta
     1501 gagtggatgc aacaaccaaa agcaaaagca cccaaacaaa gtgtagagga aatgattaaa
     1561 agcatcaacc aaaagtttga tgaaggcatg actaatgaag caaatatgtt ggttatttta
     1621 tctcatgata gactatttga gaagcaacaa tatgcagact ctttaagaaa atttattcaa
     1681 gttttaaaaa gtgatcctag aaatgtattt gaaacaatag accactatcc attggttcaa
     1741 aacaaataaa atatatttag gatgattgat tttaggagcg atacagttac caaaccaact
     1801 aagcaaatgc tggaagcaat gcttcaagca aaagttggag acgatgtttt tggcgaagac
     1861 ccaacagtaa acgaattaga agaatatgct gctaatttat ttggtattca agcagcattg
     1921 ttttgtccat caggaacaat gaccaaccaa attgctataa aagttcatac ccaaccagga
     1981 gatgaaatta tttgcgataa cttaagtcat atttatcaat atgaaggtgg aggtattgca
     2041 gtaaatagtg cttgttctgt aaaactaatt aatggtaatt atggtaaaat taatgctaag
     2101 caagtagcag aaaatattaa caatataaat gatgttcata agccaataag caaattgctg
     2161 agtctagaaa atacttccaa tcgtggtggc ggtagttgct accattttga tgattttatt
     2221 gcaataaaag aagtatgtac tcaaaataat ttaatgctgc atttagatgg tgcaagattg
     2281 tttaatgcaa taatctataa aaatgaatca gcaaaacaat atggcaaagt atttaactct
     2341 atttctatat gcctaagcaa aggattgggt gccccaatag gcagcctatt attaggcgat
     2401 aaaaatttta ttttaaaagc ccgtagagta agaaaggttt ttggaggagg aatgagacaa
     2461 gcaggtataa ttgcagcagc aggcttatat gctttacaaa ataatataca aagactttct
     2521 acagaccacc atcatgctca gcttattgcc acagcattaa ggcaaaaaag ctttgtaact
     2581 aatattttac cagtagaaac caatattatc atatttgaag tagctgaaaa atatactgca
     2641 aaagagttaa tagccactct ttataaaaat aatattttag cttttgccat agcagaaaat
     2701 caaattagat tagtgttaca tttaggcata acaactgaaa tggttgaaga aaccatacaa
     2761 ttaattcaaa aattataata cttatttttg caaaatgcaa caacccacta acgagcagtt
     2821 tttagagatt gctcaacaat ttggtacacc agtttatgta tatgatgcta ataaaattgt
     2881 aagtcaatat caaaaattta aatctgcttt taacagccct actgtaaaaa tattttatgc
     2941 ctgtaaggca ctaactaata tcaatatttt aaaactttta aaacttcaag gtgccaatat
     3001 tgattgtagc agcattaatg aagtaaagct tgcaatacat gctggttttg agccccaaca
     3061 aatactatat acatctaacg gaattgggtt tgatgaaata gaagaagcca aaaaccttgg
     3121 tattcacata aacattgata gcataagtaa tttaaaaaag tttggacaaa aatttaataa
     3181 ctcctaccct gttggtgtta ggttaaggcc caatataatg gctggaggaa atataaaaat
     3241 tgctacaggt catgataaaa gcaaatttgg aattcctata acccaaattg ccgaagtagt
     3301 ggatataatg cagcaatata atttattaat taatggtgtg catatacata caggaagcga
     3361 tattaaagaa gcagatgttt ttttaaaagg tattgaagtg ttgtttgatg taatccattt
     3421 atttcctaat ttaatgtatg ttgatttagg cggtggcttt aaagttcctt ttgaagatac
     3481 ggaagaagaa actgatatga tcacattagc aaataaaatt cttgaaaaat tcaatcaaca
     3541 cttacatcaa aataaattac aaatttggtt tgaacctgga aaatatttag tgagccaagc
     3601 cggatatttt ttaacaaaaa ttaatgtgct caaaaaaacc aataatattg aatttgcaag
     3661 tatcaattct gggtttaatc atttattacg ccctatgttt tacgatgcat accataaaat
     3721 tgtaaatatt agtaacccca atggcactat taaagaatac gatattgttg gaaatatttg
     3781 cgaaacagac acatttgctg caaacagaaa tataaacgaa attaacgagg gtgatttatt
     3841 ggttttttat aatgccggag cttatggttt tgaaatgagt agcaattata atagtagata
     3901 taaaccagca gaagttttaa ttcataacaa tcaaattagt ttaattagaa aagcagatag
     3961 gtttgaagat ttattaaaaa atcagattgt tgttttataa aaaatctaac taaattatca
     4021 tcttaacaat attaattttt atccatttat tttaacttac tataagttta ttaatagttt
     4081 attattactt aaataacact acttttgccg ccaaattaga aaaaggaagt aagtatgtta
     4141 agtgcaaaaa atattactct tgcttatggt aaaagagtgc tgtttgatga agtaaatatc
     4201 aattttacaa aaggcaactg ttatggtgta ataggtgcaa atggtgctgg taaaagtacc
     4261 tttctaaaaa ttttaagtgg tgaaattgaa cccaataaag gaactgttga aattggtaaa
     4321 ggtgagagaa tgagcttttt aaaacaaaac caatttgagt ttgatgaaga aactgtttta
     4381 aacactgtat taatggggca taagcaaatg atggaattaa tgcaaaaaaa agatgccatt
     4441 tatgctaatc cagatgcaac cgaagaggat tatatgaaag caggtgaatt agaagcagag
     4501 tttggagaaa tgggaggcta cactgccgaa agtgatgcag cagctttatt aggcagctta
     4561 ggtgttaaag aagagtttca taatagctta atgaaagaca taccaggcaa tttaaaagtt
     4621 cgtgtattac ttgcacaagc actatttggc aatccagata ttttattatt agatgagcct
     4681 accaatggat tagatattga aacaattggt tggcttgaga attttttagc cgattatgaa
     4741 aatatagttt tagtagtaag ccatgacagg cactttttag atgcagtttg tacacatgtt
     4801 gctgatgtgg acagacaaaa aataaaaacc tttacaggta actatacatt ttggtatgag
     4861 tcctctcaat taatgtctcg tcaaataaat gataaaaaca aaaaagttga agaaaaacgc
     4921 caggccttga ttgatttcat tgcacgtttc tctgccaatg catctaaaag taaacaagct
     4981 acttctcgta aaaaagcttt agaaaaatta actatagagg aaattgaacc aagtaataga
     5041 aaatatccgg gaattatttt tcaaccttta agagaggtag gtaatcaaat tttaaatgta
     5101 gaaaatttaa gcaaaaccat agatggaaga accttattta gcaaagcaac ttttagtgta
     5161 aataaaaatg ataaaattgc ctttttaagt aaagacccct tagctgttac taactttttt
     5221 gaaataataa acgataacat taaaagtaat gaaggtaagt ttgaatgggg aactaccatt
     5281 acaaaagcat acttacctat ggaaaataat gaatttttta cagagggttt aagtttaatg
     5341 gaatggcttc gtcaatttgt accagcacat gtaacagatg cagatgaacc ttttttaaga
     5401 ggctttctag gaaaaatgtt atttagtgga gatgatattg ctaaaaaaac aaacgtatta
     5461 agtggaggag aaaaagtgcg ttgtatgatt agcagaatga tgcttcaaaa ccctaatttg
     5521 gttgtattag accaaccaac aaaccattta gatttagaaa gtattcaaag ctttaacgaa
     5581 ggttgccaaa cttttccagg aattgtatta ttaaccagcc acgaccacac ttttatacaa
     5641 acagttgcca atcgtattat tgagttaaca cctaaaggaa ttatagacag attaatgact
     5701 tttgatgaat atatggatga taaaagagtg caggaattaa gaattgaaat gtatagttaa
     5761 ataacataat tacattaaaa taatacttta aagaggttgc ttaaaacaat aagcagcctc
     5821 tttaactttt tataaggctc ctaattattt ttgttttttt cataggtaag ttagaagcta
     5881 agcaatttcc ttcaaataaa aaaggttgta atttgtatta caacctaaat aatattttat
     5941 tgaaacttag cactattcaa tgttaaaatt cattttaata tcgctcttat tatcttttaa
     6001 taatgctata tatttttttg caatttcagc atcaacaact tttccattaa ttgtaattat
     6061 accattctca tagcttaaac tagctttgct ttgtagtaag ccgtcctttt gtaaggctcc
     6121 cacaaaatta gtatagttgt tttcagaaat ggatgtggtg gttgttattt caatcttttt
     6181 atcaactgta actgtttcat tggaatgaag ttgagccaag gtaggtgcaa ttaccaatga
     6241 tacaatactc attagtttaa ttaaaatatt cattgatggg ccagaagtat ctttaaatgg
     6301 atcaccaact gtatctcctg taacagatgc tttatgaggt tctgattttt tgtaaaacat
     6361 ttcaccatta atatttactc ctttttcaaa agattttttt gcattatccc atgcaccacc
     6421 agcattattt tgaaacattc ctaacaatac accacttact gtagcacctg ctaaaaatcc
     6481 acctaaagct tcggagccta aagtaaaacc aataattatt ggagatagaa ttgcaatagc
     6541 accaggtaac atcatttttt taatggatgc ttcagtagat attgctacac atttatcata
     6601 ttcaggtttt ccttttcctt ccataatacc aggaatggtt ttaaattgtc ttcttacttc
     6661 ttcaaccatt gccattgctg cttctccaac agctctaata gccaaagaag aaaagataaa
     6721 aggaatcata gctcctacaa ataaagtagc taatactttt gctttataaa tatcaatacc
     6781 agtaatttta gataccccaa caaatgcagc aaataatgcc aatgatgtta aagcagcaga
     6841 tgcaatagca aaaccttttc ctgtagcagc agtagtatta ccaactgcat ctaacacatc
     6901 cgttttttca cgaacatcgc ttggtaactc actcatttct gcaataccac ctgcattatc
     6961 tgcaattgga ccaaatgcat ctatagctaa ctgcattgca gttgtagcca tcatacctgc
     7021 agcagcaatt gcaacaccat ataagcctgc acattcataa cttccccaaa tacctccagc
     7081 taaaactaaa attggtaaaa atgtactttc cattccaatt gctaaaccac caataacatt
     7141 ggtagcatgg ccagtagcac tttgtctaat gatagacatt acaggtcttt tacccattgc
     7201 agtataatat tcagtaataa tactcattaa agcaccaacc actaatccta cgcctatggc
     7261 acctaatacg ccccatttag taaactcaaa acctctaagg ctcatagttt ctggtaaaat
     7321 ataataaact aaacctgcac aagccaaagc agttaaaata attgaacccc aattacccat
     7381 atttaatgct ttttgaacat tagctgtgtt aattcctgca gcatcactaa ctcttacaaa
     7441 ccatgttcca acaattgaaa ataaaatacc aatacctgca ataagcattg gcaataaaat
     7501 aggtgcatag ccaccaaaat tatcaataga agaagtttct tgtcctaata ccattgttgc
     7561 taatacagta gctacataac taccaaataa atctgcaccc atacctgcta catctcctac
     7621 attatcacct acattatctg caatagttgc tgggtttctt ggatcatctt caggtattcc
     7681 ggcttctact tttcctacta aatcggcacc aacatctgct gcttttgtgt atataccacc
     7741 acctacacga gcaaataatg caatgctttc ggcacctaat gaaaatcctg ttaaaacttc
     7801 aatagtttta agcatttcta atgagtttac agcctctggt gcaaacatgc cttttaatat
     7861 aataaataca cctcctaatc ctaaaacagc taaacctgct acaccaacac ccattactgc
     7921 tcctcctgta aatgatacag ataaagcttt tgataaactt gtacgagctg cctgtgctgt
     7981 acgtacattg gcttttgtag caaccttcat acctatataa cctgcaactg cactacaaat
     8041 tgctcctaaa ataaaggcta ttgcaatact ccaatggcta tgtggatttt tagtagccat
     8101 aaagcctaat aacaaggcta caataattac aaaataggtt agtattttcc actctgcttt
     8161 taaaaatgcc atagcacctt ctgcaatgta attactaatt tctttcattc tttcgttgcc
     8221 agcatcttgt ttggcaaccc agttaaactt aataaatgtg taaagcaaac caacaatacc
     8281 taaaataggt actattaaaa ataacatgtt agtagtcata agcaagtata tacttaaaaa
     8341 tttaaaatgg gtggcaaaaa tagggggata tttattataa atatcagttt aaatgaacta
     8401 attcataagg tttttattaa tttcaaattt tagtattgta taaaataaat tcagttgttc
     8461 catttgttct acccttttaa aattatatta aaaaggctac ccttaaaaag ggtagccttt
     8521 agtaattaat atgttgtatt aattataagt tataaccgta acttatatag taaacaccac
     8581 ctacttgagg gttaccaaat gcacttctat aatacttatt ccagaagttt aaagcaccaa
     8641 ttttaaataa tcctttgtta gctgttttgt agctaaccat agcatcccat gtgccataag
     8701 aaggaataac acctgtacca aaagtacctt cccaattcac tttatcttgc catctgtgca
     8761 caataccaaa gccccaattt ttatatacat tggtgttact taagccaata ttaaatctca
     8821 ttttaggagt attaaattgg gtaaatactg tagaaggaac atcatttaat tggtcgccag
     8881 aaacgtttgc attaaataca aatccttttg gtaacatata atcagcacta ataccccatc
     8941 caattgcttt tacattgcta gcagtattag taacaaaaga ataattattg ctgcttgatg
     9001 ggcttaaaag ttccatttga gtaccatttt gacctcttgc tactgctacc cttcctaaaa
     9061 agttattgta ttcactaaaa tagaaataag catctatcat taatttattt ttaataactc
     9121 ctctgtagcc aatttcgtat gattttacag tttctggttt aactgcttgg aaagtacctt
     9181 taacaagtac tgttgggtca attgcaccag ttgctgcaaa tttagcacgg aataaaccaa
     9241 cactttccgc tgtataaata ggatttgtac taaagccata tacatcatta aatattggta
     9301 atgcaccaat taattttgca cttgggctgt ttaggttaat ccattggtct tgtgtagaag
     9361 gaaaacgata agctgtttga aaggatactc ttatgttatt gtcttttgca actttgaata
     9421 aagcagttgc tcttggtgta aatctaccat caaagttttg gtttttatca tacctgatag
     9481 aacctgtaag ttttaagaaa tcatttactc ttttagcaac ttgaacatac ccacctactt
     9541 cgctaatatt aatatttcca tttttaaaat taggtgtatg gtcagcaaaa atagtaccac
     9601 cagaatttag gttaaatact ctgtagcttg caccaaccaa tacttctgcc acttttattt
     9661 tatcggttaa attgtactgg ccttcaaaat ggtatagttt acttttatct gcaaataagc
     9721 ctccaccatt agtaattgaa gttcctgtta cttgttcaaa cgcattcata aagcctgtag
     9781 aacctggcat atatcttcct tggtctgcag ctgcacgtcc ttgtgcatga gctaaagtgg
     9841 catttccatt ggtacctaac attgtttgtg tataagcagt catgtattga gaaaaccaag
     9901 tagcatcagc tttccacatt ctgttcattt gaactgcagt aatagtgttt gcataagaat
     9961 cgccagaatt ttcagaaatt aagtagcctc ttaaaaacca atttttagct tttaattctg
    10021 ctttatactg acccattttt aaattcttta agctatatct a
//
LOCUS       DAOQPK010000065         9977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_3581_length_9977_cov_4.739871, whole genome shotgun sequence.
ACCESSION   DAOQPK010000065 DAOQPK010000000
VERSION     DAOQPK010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9977
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3581_length_9977_cov_4.739871"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="PLU36_04625"
     CDS             <1..451
                     /locus_tag="PLU36_04625"
                     /inference="COORDINATES: protein motif:HMM:NF037084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HMZ46066.1"
                     /translation="EIGVLSQILPYDIELEKFIKKVQIEARVMGDLALNHIIPVAVKY
                     QNELLNNINGLKQIGLPAKNYAVQLKIVEEISNHLQQVYNNADALVEARKVANKIEDT
                     RTKAIAYDTDVKAKYFEILRYHVDKLEHLVCNNEWTLPKYRELLFVK"
     gene            complement(672..2348)
                     /locus_tag="PLU36_04630"
     CDS             complement(672..2348)
                     /locus_tag="PLU36_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HMZ46067.1"
                     /translation="MSLFRKKSLDKINIEFTESHHQQGLKRVLNVKDLTFMGIAAVVG
                     AGIFSTIGKASFDGGPGVVFLFLITAVTCGFSALCYAEFAGRVPISGSAYTYAYVSFG
                     EIIAWIIGWALILEYAIGNIVVAISWSGYFDNLLQGLGVHLPKWLLADPFTASETFKA
                     AIAEQASGKPLSAAMQEAINIYNSAPIIAGKHIFFNLPAFIIVVLITLLAYVGIKESK
                     KSANYMVIFKIAVIIFIIVMGFFYVDAKNWNPFLPNGFGGVLAGVSAVFYAYIGFDAI
                     STTAEECKNPQRDLPKGMIYSLLICTVLYILIALVLTGMVNYTELNVTDPLAFVFEKL
                     GLHKIGYIISISAVVATTSVLLVFQLGQPRIWMSMSRDGLLPKRFAKVHPKFGTPSFA
                     TIVTGLLVGIPTLIMPADLVTDLTSIGTLFAFVLVCLGVLVLPKLSKEQTKGRFRLPY
                     INGKFIIPALVILFIWFTKERIENSFLQIGSESYHEILFLIFILISVVIAVFSFIKNY
                     SLIPIIGALCCMYLMIEIPAESWIWFFVWMAIGLSIYFMYGRKRSRLNNA"
     gene            complement(2500..3465)
                     /locus_tag="PLU36_04635"
     CDS             complement(2500..3465)
                     /locus_tag="PLU36_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HMZ46068.1"
                     /translation="MHSFKELVNIFATHFNQAHFPDNPNSLYEPCNYFLTLGGKRIRP
                     IMCLMGNELFDEIKPDAFYAATAIELFHNFSLIHDDIMDAASLRRGMQTIHTKYNPNT
                     AILSGDVMLIRCYDYINKIQPIYLPKVLHLFNKTAREVCEGQQLDMDFEKLSAVSLED
                     YIKMISLKTSVLIASSLEIGAVLGGAGEGNCKKIYDFGINLGIAFQIQDDYLDAFGDP
                     EKFGKQVGGDIKQNKKTFLLLHALEVANQNQREELINLLQTDSSDKVERVTTIFKACN
                     VDAWANELKEKYVQTAFKNLEEIAVTSVRKKYLQELASFLIKRDI"
     gene            3525..5141
                     /locus_tag="PLU36_04640"
     CDS             3525..5141
                     /locus_tag="PLU36_04640"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HMZ46069.1"
                     /translation="MKYVFKNEFTIAIFAAILFIPFIGHVHLFDWDEINFAESAREMI
                     ATGNYFDVQINYSKFTEKPPLFFWMQVLSMKAFGINEFAARFPNAVCGILTLVLLFRI
                     GKKIYNENLARIWVLTYLGTLVTFLYFKSGIIDPWFNLFIFLAIYHYYLLITYENSKT
                     KQAVLVGLFLGLAVLTKGPVAILIAMLCMLTYVVLNKGKFNISTKNFILILLSCLVVC
                     FAWFGIDLLKNGPAFIIEFTQRQVAIFSTSDAGHGQPFFYHWWVLLVGCFPASIFFIS
                     GQMIQNGNKEQKEFKLWMVILFWVTLLLFSIVKTKIIHYSSLCWFPLTFIAALFIYEL
                     WEGRIKKIHTLLKILFALVGVTLGLIITVLPIVLQYKNKWVHYIKDDFAKGNLQANVQ
                     WYWIDGVGGLLLLFSVVYFLCSKKIKLKTQLLYASVTVCCFFTAVLIAPKVEAISQRA
                     NIEFFESKQNEDCYIDTWGYKSYAHFFYSKKQPANFDSTKNMQWMLYGKIDKPVYIST
                     KIQNKKILDTVQNLKMLYQKNGFVFYNRLP"
     gene            5196..5603
                     /locus_tag="PLU36_04645"
     CDS             5196..5603
                     /locus_tag="PLU36_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HMZ46070.1"
                     /translation="MNRPIRVLVAKVGLDGHDRGAKVIATALRDAGMEVIYTGLRQTP
                     EMVVNAALQEDVDAIGVSILSGAHKTVFPKIMHLMKEKNMNDVLLTGGGIIPEDDMND
                     LNELGVGKLFAPGTNTSEIVKYINEWVKKHRNF"
     gene            5758..5955
                     /gene="rpmI"
                     /locus_tag="PLU36_04650"
     CDS             5758..5955
                     /gene="rpmI"
                     /locus_tag="PLU36_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009617012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HMZ46071.1"
                     /translation="MPKVKTNSSAKKRFKVTGTGEITFQKAFKRHILTKKSKKRKRNM
                     RKAGVVGSANKDFVQRLLGLK"
     gene            6046..6390
                     /gene="rplT"
                     /locus_tag="PLU36_04655"
     CDS             6046..6390
                     /gene="rplT"
                     /locus_tag="PLU36_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HMZ46072.1"
                     /translation="MPRSVNAVASRARRKRILKQAKGFYGKRKNVYTVAKNIVEKGMT
                     YSYVGRKLKKREYRQLWIARINAAVREEGLTYSQFINKLSVKGIDLNRKVLADLAMNN
                     PESFKALVASVK"
     gene            6518..7948
                     /locus_tag="PLU36_04660"
     CDS             6518..7948
                     /locus_tag="PLU36_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HMZ46073.1"
                     /translation="MTNSYTSLVHQTFHFPQEDFNISDKNDFLEFNGINLKPIIEKYG
                     TPLKLTYLPKIGMQINKAKQMFANAFKKHKYEGKYNYCYCTKSSHFSFVVEEALRHNI
                     NIETSFAYDIEIINQLYKRRKITKDVFIICNGFKQKAYTSRIAKLINSGFKNVVPILD
                     NKEELKAYKKTVKQPFGIGIRVAAEEEPNFPFYTSRLGIRAKDILEFYVDEIEGNESK
                     FYLKMLHVFLNKGIKDDIYYWSELNKVINLYCQLKKICPELDSINIGGGFPIKHSLGF
                     EYDYQFMINEIVSNIKKSCKKAKVPVPNIFTEFGSYTVGESMAHIYSIIGEKQQNDRE
                     TWYMIDSSFITTLPDTWGIGEKFLMLPINKWDNEYQRVVLGGITCDSHDYYDSEEHIK
                     EVFLPKIKSNKPEDDANKEPLYLGFFHTGAYQDQISGYGGIKHCLIPSPKHVIIEHDK
                     NGNLIDWVYAKEQSASSMLKILGYLK"
     gene            7968..8702
                     /locus_tag="PLU36_04665"
     CDS             7968..8702
                     /locus_tag="PLU36_04665"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HMZ46074.1"
                     /translation="MKRVIILIAMCPLIAIAQQTKTTPKKVVQQTKTTSSKPTKGFIL
                     KTGLDSLSYALGTRIALNLNANHIDTLSYNAFIEGLKDIFKKSNLKFDENTINQVINT
                     KVQELSVQKASKEKENGKKFLEANKKKQGVITTSSGLQYEVITVGNGAKPTLQDTVVC
                     HYVGTLVNGFEFDNSRKRGEPIQFPVTGVIKGWTEALQLMPKGSKWKLYIPSDLGYGD
                     RGAGNDIPGGSALIFEVELIDIKAKQ"
     gene            8852..>9977
                     /locus_tag="PLU36_04670"
     CDS             8852..>9977
                     /locus_tag="PLU36_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46075.1"
                     /translation="MKKQFLLFFILFSVSFIKINAQTDVIIGAGSVTGTTANGAATDA
                     GPIYCTGSSSSFIYSKHFIVYTAAELSNAGIQPGMLITNLAWNKANDAAYSASTAVVF
                     DIYMKNSSATGVPTPVPQDFASLVSGATLVYASTTQSFPSTIGWVNFTLTTPFLYTGG
                     AIEMATNWDVSAGGSTASGATANFAWAKDPGFNILSYCAATQSPNLTNLRTSRAQIKI
                     TFTPGGSCTVPPIAGTANSNKTNTCIGETFSLGLTGNSSGIGQTYKWQSSLTGNAPWT
                     DVSLAQASPSFSTNQNISTYYRCMVTCSGNSVYSDSLQILTPVAVNGTYSINSALPTG
                     GSNFATFAEAFDYIKCGINGPVVFNVAVGSGPYALSAPLNI"
BASE COUNT         3666 a         1461 c         1521 g         3329 t
ORIGIN      
        1 tgaaatagga gttttaagtc aaattcttcc ctatgatatt gagcttgaaa aatttattaa
       61 aaaagtacaa atagaagcaa gggtaatggg agatttagca ctgaaccata ttatacccgt
      121 tgcagtaaaa tatcaaaatg aattactgaa taatatcaat ggcttaaagc aaattggatt
      181 accagcaaaa aactatgctg tacagcttaa aattgtagaa gaaattagca accacttaca
      241 acaagtttac aataatgcag atgctttggt tgaagctaga aaagtagcaa ataaaattga
      301 agatacgaga acaaaagcaa ttgcttatga tacagatgtg aaggctaaat actttgaaat
      361 actacgttat catgtagata aattagaaca tttagtttgc aataatgaat ggactttacc
      421 taaatataga gaattgttat ttgtgaagta aacagttatt ttaaccaata aattaagcat
      481 cgttattata ataaataacg gtgcttttta tttacaataa tgtacaatta aaactttttg
      541 atgtgaattg ttaatgcaca aagttaatat tccttacatg cttttgcact ttatcaatgc
      601 ttgcacaaaa gacgagataa ttaaaaaaaa tatttacaga aaacacctaa ataaaatata
      661 aacattcaca tttatgcatt atttaacctg cttctttttc taccatacat aaaataaata
      721 cttaaaccaa ttgccatcca aacaaaaaac caaatccaac tttctgcagg aatttctatc
      781 attaaataca tacaacataa agcaccaata attgggataa gagaataatt ttttataaaa
      841 ctaaaaactg caataacaac agagataagg ataaaaatta aaaataaaat ttcgtggtaa
      901 gattcacttc ctatttgtaa aaaagaattt tctattcttt ctttagtaaa ccaaataaac
      961 aatattacta aagctggaat aataaatttg ccattaatat aaggtaatct aaaccttcct
     1021 tttgtttgct ctttacttag tttaggtaaa actaaaacgc ctaaacaaac taatacaaat
     1081 gcaaacagag taccaatgct tgtaaggtca gttacaaggt cggctggcat tattaaagtt
     1141 ggtattccaa ctaataaacc tgtaacaata gttgcaaacg aaggggtgcc aaatttagga
     1201 tgtactttag caaatctttt aggtaataat ccatctctgc tcatgctcat ccaaattcta
     1261 ggttgaccta attgaaaaac taataataca ctggtagttg caactactgc acttatggaa
     1321 attatataac ctattttatg taagcctaat ttttcaaaaa caaaagccaa tgggtcggtt
     1381 acatttaatt cagtataatt aaccatgccg gttaaaacca atgcaattaa tatgtataaa
     1441 acagtacaaa ttagtaaaga gtatatcata ccttttggta aatctctttg tggattttta
     1501 cattcttctg cagtagtact tattgcatca aagccaatgt aggcataaaa cacagcagat
     1561 acgccagcta aaacaccacc aaagccatta ggtaaaaatg ggttccagtt ttttgcatcc
     1621 acataaaaaa atcccattac tataataaaa atgataactg caattttaaa aattaccata
     1681 tagttagcac tctttttact ttctttaata cctacataag caagtaaggt tatcagtaca
     1741 acaataataa atgcaggcag attaaagaaa atatgtttac ctgcaattat tggagcacta
     1801 ttgtaaatat taatagcttc ttgcatagct gcagataatg gttttccact agcttgttct
     1861 gcaatggcag ccttaaaagt ttcagatgca gtaaaaggat cggctaataa ccatttgggt
     1921 aaatgaactc ctaaaccttg taataaatta tcaaaatatc cactccaact tattgcaaca
     1981 actatattac caatagcata ttctaaaatt aaagcccaac caattatcca tgcaattatt
     2041 tctccaaagc ttacataagc ataggtgtaa gcactgccag aaattggaac tctgccagcg
     2101 aactctgcat aacacaaagc actaaaacca caggttacag cagttattaa aaataaaaat
     2161 acaacaccag gtcctccatc aaaagatgct ttgccaattg tagaaaaaat accagcacct
     2221 acaactgctg caatacccat aaaggttaaa tctttaacgt ttaaaaccct ttttaaacct
     2281 tgttgatggt gcgattcagt aaactcaata ttaattttat ctaaagattt tttacggaat
     2341 aaagacatat agctttagtt ttagttggtt ttaatacatt gctaatataa gtagtattcc
     2401 aataaaatat aaattttaga ataatgttat taagctataa ttaaatgaca ggagtttaat
     2461 ttttacaaca caattcttat taaaccaaaa tggctgttat taaatatctc gtttaattaa
     2521 aaacgatgca agctcttgca aatatttttt tcttacagag gttactgcaa tttcttctaa
     2581 atttttaaat gcagtttgta catatttttc ttttaattca tttgcccagg catctacatt
     2641 acatgcttta aaaatagtag taactctttc aactttatct gaagaatctg tttgtaataa
     2701 attaattaat tcttctcttt gattttgatt ggcaacttca agagcatgca gcagcaaaaa
     2761 tgttttctta ttttgtttaa tatcgccacc aacttgctta ccaaattttt ctgggtctcc
     2821 aaatgcatct aaataatcgt cttgtatttg aaaggctata cctaaattaa ttccaaaatc
     2881 ataaatcttt ttgcaattac cttctcctgc accacctaaa acagcaccaa tttctaaact
     2941 agaggctatt aataccgatg tttttaaaga aatcattttt atataatctt ctaaacttac
     3001 ggcagataat ttctcaaaat ccatatctaa ttgttggcct tcgcaaactt ctctagctgt
     3061 tttattaaat aaatgaagca ctttaggcaa ataaattggc tgtattttgt tgatgtaatc
     3121 ataacatctt attagcatta catctccact taaaatagca gtattaggat tgtattttgt
     3181 gtgaattgtt tgcattcctc ttcttaaaga ggcagcatcc ataatatcat catgtattaa
     3241 agaaaaatta tgaaacagtt ctattgcagt ggctgcataa aatgcatctg gtttaatttc
     3301 atcaaataat tcattaccca ttaaacacat aatgggtctt attcttttac ctcctaaagt
     3361 taaaaagtaa ttacatggtt catataagct gttaggattg tctggaaaat gtgcttggtt
     3421 aaaatgagtt gcaaaaatat ttaccaattc tttaaacgaa tgcatgaaac tttattttca
     3481 acaaaaataa ggaactaagc tgtcttactt acttttgcac taacatgaag tatgtattta
     3541 aaaatgaatt taccattgca atttttgcag caatactttt tattcctttt ataggccatg
     3601 ttcatttatt tgattgggac gaaataaatt ttgcagaaag tgccagagaa atgattgcaa
     3661 caggtaatta ctttgatgtt caaattaatt attccaagtt taccgaaaag cctccattgt
     3721 ttttttggat gcaagtatta agtatgaaag cctttggtat taatgagttt gctgctcgat
     3781 ttccaaatgc tgtttgtggt attttaactt tagtattact atttagaatt ggtaaaaaaa
     3841 tttataacga aaacctagct cgtatttggg tgctaactta tttaggcact ttagtaacct
     3901 ttttatattt taaaagtggt attatagatc cttggtttaa cctatttatt tttttagcta
     3961 tttaccatta ttatctttta ataacgtatg aaaatagtaa aaccaaacaa gctgttttag
     4021 ttggactttt tttagggctt gctgttttaa ctaaaggccc tgtggcaatt ttaattgcaa
     4081 tgctttgtat gctaacctat gtagtattaa acaaaggtaa atttaatatt tcaactaaaa
     4141 attttattct tatcttactt agttgtttag tcgtttgctt tgcatggttt ggaatagatt
     4201 tattaaaaaa cggtccagca tttatcatag aatttacaca aaggcaggtt gccattttta
     4261 gtactagcga tgcaggacat ggccaaccat ttttttatca ttggtgggtt ttattagtag
     4321 gctgttttcc tgcttccata ttttttataa gcgggcaaat gatacaaaat ggaaacaaag
     4381 aacaaaaaga gtttaaacta tggatggtta ttttattttg ggttacctta ttattgtttt
     4441 caattgttaa aacaaaaatt attcattact cttccttatg ttggtttcca ttaacattta
     4501 ttgctgcatt atttatatat gaattatggg aggggagaat aaaaaaaata cacactctat
     4561 taaaaatatt atttgcttta gtaggggtaa cattaggctt aataattact gttttaccca
     4621 tagtattaca atataaaaat aaatgggtac attatataaa agatgatttt gcaaaaggga
     4681 atttgcaagc caatgttcaa tggtattgga tagatggagt aggaggtttg ttacttttat
     4741 tttctgttgt atattttttg tgtagtaaaa aaataaaatt aaaaacgcag ttattgtatg
     4801 ctagtgtaac tgtatgttgt ttttttactg ctgtattaat tgcacctaaa gtagaggcaa
     4861 tatctcaaag agcaaatatt gaattttttg aaagcaaaca aaacgaggat tgttatatag
     4921 atacttgggg ttacaaaagt tatgctcatt tcttttattc aaaaaagcaa ccagcaaatt
     4981 ttgattcaac aaaaaatatg cagtggatgt tgtatggaaa aattgataaa ccagtttaca
     5041 tatctacaaa aattcaaaat aaaaaaatat tagatacggt acagaatcta aaaatgttat
     5101 accaaaaaaa tggctttgta ttttataata gattgccata aaacaaaatg aatattttaa
     5161 ataattactt ttacactata ttatattaaa taattatgaa tagaccaata agagttttag
     5221 tagcaaaggt tggattagat ggacatgata gaggagcaaa agttattgca actgctttaa
     5281 gagatgctgg tatggaagta atatatactg gtttaaggca aactcctgaa atggttgtaa
     5341 atgctgcctt gcaagaagat gtggatgcta taggtgtaag tatattaagt ggtgctcata
     5401 aaacagtgtt tcctaaaata atgcacttaa tgaaagaaaa aaacatgaat gatgttttgc
     5461 taactggtgg aggtataatt ccagaagatg atatgaatga tttaaatgaa ctaggtgtag
     5521 gtaaactttt tgcaccaggt actaatacaa gtgaaattgt taaatacatt aatgaatggg
     5581 tgaaaaaaca tagaaatttc taatacttct aatataattg ctatattctt acttttttgt
     5641 tgtttatttt agtataaact cctaattttg caatcccaaa aatattaccc acaaggctca
     5701 acaagtttct cgtctgttcg agaacgccag cagggtgtaa tcttaaccag attatttatg
     5761 cctaaagtaa aaacaaactc aagtgcaaaa aaaaggttta aagtaaccgg cacaggcgaa
     5821 attacttttc aaaaagcgtt taaacgccac atcctaacta aaaaatctaa aaaacgcaaa
     5881 cgcaacatgc gtaaagcagg tgttgtaggt tctgctaata aagattttgt tcaaagatta
     5941 ttaggattaa aataaacact taaacaagaa aagtaattaa ccaaattcat ttcacttttt
     6001 cggtttttaa ttattaaatt ttaccgaaat taaattaaac caattatgcc acgttcagta
     6061 aatgcagtag cttctagagc tcgcagaaaa agaatattaa aacaagcaaa aggcttttat
     6121 ggcaaacgta aaaatgtata tacagttgcc aaaaatattg tagaaaaagg gatgacttac
     6181 agttatgtag gtcgtaaatt aaagaaaaga gaatatcgtc aattatggat tgctcgtatt
     6241 aacgcagcag ttagagaaga aggtttaact tatagccaat ttatcaataa gttatcagtt
     6301 aaaggaatag atttaaacag aaaagtactt gcagatttag ctatgaataa tcccgaaagc
     6361 tttaaagctt tagtagcttc tgtaaaataa tttttttaaa taaagttata tttttagccg
     6421 aagaaaattt tcttcggctt tttaattttg catacttcaa aattgtattt ttgttactta
     6481 ctttttaatt ttaatgtttt aatagaagtt attaagaatg acaaacagct acacctccct
     6541 ggtgcatcaa acatttcact ttcctcagga agactttaac attagtgata agaatgattt
     6601 tttagagttt aatggcatta accttaaacc aataatagaa aagtatggaa cgccattaaa
     6661 actaacctat ttacctaaaa taggaatgca aataaataag gcaaaacaaa tgtttgccaa
     6721 tgcttttaaa aaacataaat acgaaggaaa atacaattat tgttattgta ccaaaagctc
     6781 tcatttttct tttgtggtgg aagaagcatt aaggcacaat attaacattg aaacatcttt
     6841 tgcctacgat atagaaataa taaatcagtt atataaaaga agaaaaatta caaaagatgt
     6901 atttattatt tgcaatggtt ttaaacaaaa ggcctacact tctagaatag ctaaactaat
     6961 aaattctgga tttaaaaatg tagtaccaat attagataat aaagaagagt taaaagcata
     7021 taaaaaaact gttaaacaac cttttggtat tggaattaga gtggctgcag aagaagaacc
     7081 caatttccct ttttatacat ctagattagg aataagagca aaagatattt tagaatttta
     7141 tgtagatgaa atagaaggca acgaatcaaa attttattta aaaatgctac atgtgttttt
     7201 aaataaagga ataaaagatg atatttatta ttggagtgaa ttaaataagg taattaacct
     7261 ttattgccag ttaaagaaaa tttgtcccga attagattca ataaatatag gtggtggctt
     7321 ccctataaaa cattccttag gctttgagta cgattatcaa tttatgatta atgaaattgt
     7381 aagtaatatc aaaaaatctt gcaaaaaagc taaagtaccg gtacctaata tttttacaga
     7441 gtttggtagt tatacagttg gcgaaagtat ggctcatatt tatagcataa taggcgaaaa
     7501 acaacaaaat gatagagaaa cctggtacat gatagattct tcttttatta ccactttgcc
     7561 agatacttgg ggtataggag aaaaattttt gatgcttcct attaataaat gggataatga
     7621 ataccagcgt gtagttttag gtggtattac atgtgatagc catgattatt acgatagtga
     7681 agaacatata aaagaagttt ttttacccaa aataaaaagc aataagccag aggatgatgc
     7741 caacaaagaa cctctttatt taggtttctt tcatacaggt gcctatcaag accaaattag
     7801 cggttatggt ggtataaagc attgtttaat tccttctcca aaacatgtaa taattgaaca
     7861 tgataaaaat ggtaacttaa tagattgggt ttatgcaaaa gaacagtctg cctccagtat
     7921 gcttaaaatt cttggttatc taaaataaat ttaaaaaaca aaattaaatg aagagagtaa
     7981 ttatcttaat tgctatgtgt ccacttatag caattgcaca acaaacaaaa acaacaccta
     8041 agaaagttgt acaacaaaca aaaacaacat catcaaagcc aacaaaagga tttattttaa
     8101 aaacaggatt agattcttta agttatgcat taggcactag aatagctttg aacttaaacg
     8161 caaaccatat tgatacctta agctataatg cattcattga agggttaaaa gatatattta
     8221 aaaaatcaaa cttaaagttt gatgaaaata ctatcaatca agttatcaat accaaagtgc
     8281 aagaattatc cgtacaaaaa gcatctaaag aaaaagaaaa tggaaaaaaa tttttagaag
     8341 ccaataaaaa gaaacaagga gtaattacaa caagtagtgg tttacaatat gaggtaatta
     8401 ctgtaggtaa tggagcaaaa cctactttgc aagatactgt tgtttgtcac tatgtaggca
     8461 ctttagtaaa tggatttgaa tttgataata gtagaaaaag aggcgagcct attcaatttc
     8521 ctgtaactgg agtaattaaa ggttggacag aagcattaca acttatgccc aaaggaagca
     8581 aatggaaatt gtatattcca tctgatttag gctatggcga cagaggagca ggaaatgata
     8641 tacctggtgg ttctgcttta atttttgaag tagaattgat agatattaag gcaaaacaat
     8701 agctacagta aacttgcata cttctattga taaactattg aattgtatat acattttata
     8761 ttggtataaa ttatatactt acacttgctt taatttttgt atttttaatg tcctgttatt
     8821 tattaaattt tcttaaccaa actaactgat tatgaaaaaa caatttttac ttttttttat
     8881 tctattctct gtaagtttta ttaaaataaa tgcacaaact gatgtgatta ttggtgcagg
     8941 ttcagtaact ggaactacag ctaatggtgc tgctacagat gcagggccta tatattgtac
     9001 aggaagttct agttctttta tatactccaa acactttatt gtttacacag ctgcagaatt
     9061 aagtaatgca ggcattcaac ctggtatgct aattactaac ctagcatgga acaaagcaaa
     9121 tgatgctgct tactctgcat ctactgctgt tgtatttgat atttatatga aaaactcttc
     9181 agcaaccgga gttccaactc cagtgccaca agattttgca tcattagttt ctggtgctac
     9241 tttagtttat gcatctacta ctcaaagttt tccatcaaca attggttggg ttaattttac
     9301 attaacaact ccttttttat atacaggtgg tgcaattgaa atggcaacta attgggatgt
     9361 ttctgcagga ggttcaactg cttcaggggc tactgcaaat tttgcatggg ctaaagaccc
     9421 tggttttaac attctttctt attgtgcagc cactcaaagt ccaaacctaa caaatctaag
     9481 aacttcaaga gcccaaatta aaattacttt tacaccagga ggctcttgca ctgtgcctcc
     9541 aattgcaggt actgcaaata gtaataaaac aaatacttgt attggggaaa cattttccct
     9601 tggtttaaca ggcaatagta gtggtatagg ccaaacatat aaatggcaat cctccctaac
     9661 aggcaatgca ccttggacag atgtaagttt agctcaagct tctccaagtt tcagtactaa
     9721 tcaaaatatt tctacatatt atcgttgtat ggtaacttgt agtggcaatt ctgtttattc
     9781 cgattcttta caaattttaa cacctgtagc tgttaatggt acttatagca tcaatagtgc
     9841 cttacctact ggtggcagta attttgcaac ttttgcagaa gcttttgatt atataaaatg
     9901 tggcattaat ggcccagttg tatttaatgt agctgtaggt agtggtcctt atgctctttc
     9961 agcccctcta aatatac
//
LOCUS       DAOQPK010000066         9909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_3613_length_9909_cov_4.735336, whole genome shotgun sequence.
ACCESSION   DAOQPK010000066 DAOQPK010000000
VERSION     DAOQPK010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9909
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3613_length_9909_cov_4.735336"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PLU36_04675"
     CDS             <1..228
                     /locus_tag="PLU36_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ46076.1"
                     /translation="LIITDIMMPYTSGLELVGIVKANLNKKTPIIVLSGMGEENIVLQ
                     AFEMGADDFLTKPFKPSELSLRVKRLEMKLR"
     gene            328..401
                     /locus_tag="PLU36_04680"
     tRNA            328..401
                     /locus_tag="PLU36_04680"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:362..364,aa:Ile,seq:cat)
     gene            complement(506..4024)
                     /gene="smc"
                     /locus_tag="PLU36_04685"
     CDS             complement(506..4024)
                     /gene="smc"
                     /locus_tag="PLU36_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HMZ46077.1"
                     /translation="MKLKSLEIKGFKSFADKTVVSFDEGITGIIGPNGCGKSNIIDSI
                     RWVIGEQKISALRSENLEALVFNGSKTRSASGLAEVSLTFENTKNLLPTEFSTVTVTR
                     RFYKNGDSEYRLNDVQCRLKDIHNLFLDTGVSNDSYAIIELGMVDDIIKDKDNSRRKM
                     LEQAAGITIYKTRKKEAKNKLDATEQDLSRIEDILFEINNQLKSLENQAKKAEKFYEI
                     KNEYKEIAIELAKASLEGFNLTYKELNEQQESETNRKLQLDAQIATQDAALEQEKVGM
                     IEKERVLQSMQYEFNELLQKLRTKENDKNLASQKLHYLKEKESSLIEFLNKAEGQLKN
                     TKDSIEFTQLQLKEESDKLNDLTEKVTSTRAEVDTNRSVFDGKRILVDELRNKYQQVQ
                     RTRFDAEKKVAVADTSIQNLQRTQTQLKDEKQQRETQQNQLLIELKDKETLLETKKLD
                     LEQLQEQYNKTKEQIFETQAQLESLRNELAEENRKLDSKKNEYELLKSLIDSMEGYPD
                     SVKFLHNNQAWNYKAPLLSDVIYVKEEYRTALENVLEPYLNYYVVNNLQEGLQAVQLL
                     DTNKKGKANFFLLDKLAEVKSDIHQPALTIKALDVIEVDEQYLHLAQHLLANVFIAEN
                     DEVIHHSDGAVILEKSGKYVKGKFTLTGGSIGLFEGNKIGRAKKLEKLQDEIILQQAV
                     VNALKSEIQIRHNAVIGFNEQLKENTIKQTDQEINQLTNQVFAIKNKIENIQIAQQNA
                     FQKLEEIENRLLEENNSIATTRVEFQHLTELLQQTGEELKLIEQDYQNAEQQYNAATV
                     LFNESNINLTKQQSKIATVKQELDFKQNQLTELQNQIQLNTTQLTESKDNIIEGENSL
                     IKNEEELLQLLHTKEEEAKKVNEADQTYHVLRNQLQERESELRHKIKEKENIEYLLSA
                     IKDKLNELKLNLAGMKERMNVEFKVELDEILDGARKSDDTLESLQTNSDKLKKRLENM
                     GEINPTAIEAFTEMKKRYEFILEQKNDLVQAKDSLLQTIQEVEATANQQFLETFNKVR
                     ENFQKVFKALFTEEDTADMILVNPDNLAETGVDIVAKPKGKRPSSITQLSGGEKTLTA
                     TALLFAIYLIKPAPFCILDEVDAPLDDANVGKFTQMIKKFSDNSQFIIVTHNKQTMGS
                     VDVIYGVTMQEPGVSKLVPVDFRSLN"
     gene            4322..4846
                     /locus_tag="PLU36_04690"
     CDS             4322..4846
                     /locus_tag="PLU36_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HMZ46078.1"
                     /translation="MAKNYIIIAILMIHKKYNMPSSTPIKRNEILQLISREHHYGLLL
                     SWKIREGMKKNVEINRITDYVNWFWESHLMHHFLIEEKFVFSILPTENNLVIQAINEH
                     ENLRKLFNEPEKNVETLKLIEKNLDQHIRFEERVLFNEVQLVATNEQLELIALNHTHT
                     FSDNWKDEFWATAK"
     gene            4859..6025
                     /locus_tag="PLU36_04695"
     CDS             4859..6025
                     /locus_tag="PLU36_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HMZ46079.1"
                     /translation="MVIFGIDNLLRSNCSWKKDRIALVTNHAATTNQLQPSRKLLLQN
                     GFNLVKLFSPEHGLNLMGEDGVKINNSTDELTKLPIISLYNQQLKPLEKDIDDIDIIL
                     FDIPDIGCRFYTYLWTMTYILEAAAQFNKPIITLDRPNPISGNFELAEGCLLDEKNCS
                     SFIGRFSIPIRHCCTLAELANYFNKVKNINAQLECITCSNWSRNMFQPDWKITFVPTS
                     PAIQTFNAALYYTGLGFLEATNISEGRGTQNAFEILGAPWLNNEMLCEVFNSLQDEVA
                     LQPYNFTPTFSKYKNEKCYGVSLKVKNISVYHPVHTALLLIKTIKETHSTYFAWSTYP
                     TMVNQSGEKHLDKLLGILNSENLLELSFQPFLKELNKITDSKDWQHKINSFLLY"
     gene            6120..7172
                     /locus_tag="PLU36_04700"
     CDS             6120..7172
                     /locus_tag="PLU36_04700"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase"
                     /protein_id="HMZ46080.1"
                     /translation="MKKSILLIALIGCSFLLKAQQKSYKISIIAFYNLENFYDTINDP
                     NKNDEEFTPEGPRNYNGAIYWDKVGKLATVISKLGSDEKPESLSPDGAAILGVAEVEN
                     ESVLKDLTNHPLLKKRNYQIVHYDSKDARGVDVAMLYNPKYFIVEDSKPLFVQLPGGA
                     KDAYFTRDILWVKGKLDGETVHVYVNHWPSRLGGEERSAPARNAAAMVCKKHIDSIAA
                     ADGEQKVVIMGDLNDNPTNESITQVLKAKAKIKDVRPGGLFNPWINLYKKGYGTLAYQ
                     DAWGLFDQIMISYPWLSQEQNGFYFFKQVIFNREFMTENKGRYKGYPMRTWDGNTYRG
                     GYCDHFPTYIVLLKKI"
     gene            7242..8009
                     /locus_tag="PLU36_04705"
     CDS             7242..8009
                     /locus_tag="PLU36_04705"
                     /inference="COORDINATES: protein motif:HMM:NF016952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HMZ46081.1"
                     /translation="MFIFSCNDKPKQNEHENIVQVQQLQYSVQNIYPHNQTSFTEGLE
                     WHNGFLYEGTGDTEYEGNSKLAKINLLTGNDIQKINLSKEYFGEGITLLNNKIYQLTY
                     KEQKCFVYDAKTLKKINEFTYEGEGWGMTNNGKEIIMGNGSNNLYFRNPENFAITKTL
                     NINNNYGPVTGLNELEYVDGFVYANVWPNYKIVKINLNTSKVVAEASLEDILHKHSPQ
                     DIERKVDVLNGIAYDSIGKRFFITGKYWSKVFEIQFN"
     gene            complement(8002..8907)
                     /locus_tag="PLU36_04710"
     CDS             complement(8002..8907)
                     /locus_tag="PLU36_04710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ46082.1"
                     /translation="MSVIIHQTFCPCCNSENINEAFVTNDFSVSKQIFAVWQCNDCCV
                     RFTQNIPDANSIEPYYKSANYISHSDTKEGLINKIYHSVRNFTLQTKKKLIVEQTGFT
                     NGSLLDIGAGTGAFANIMQQSGWQVKGLEPDTTASNIAFQKYGLRFQHPQALFDQEPL
                     QFDAITLWHVLEHIHDVHGYLQCINKILKQNGKVFIAVPNYTSADAAIYKEYWAAYDV
                     PRHLYHFSPLSFKKLAEQHNFLIAAYKPMWFDSFYVSMLSEKYKNNGRNNYIMAILNG
                     CISNIKTLVNVEQCSSIIYVLSKAN"
     gene            complement(9050..>9909)
                     /locus_tag="PLU36_04715"
     CDS             complement(9050..>9909)
                     /locus_tag="PLU36_04715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ46083.1"
                     /translation="YRADSKTPAAIASGTTVSLSFNARPDFILNSSLPTTFVWSPLTG
                     LFTDAAATIPYTGTSLDTVYAKPASTTTYAVSSTTAENCSVNGTVNVTVDCSVPVTYT
                     MFTGKREGMVNMLSWQTATESNNIGFELQRSSDGKQFSKLSFVNSKAINGNSTSNLSY
                     GYNDVKPLAGTNYYRLKQVDKDGKFNYSTVVILKGDKVNQISIASVYPNPVRNTINVQ
                     IVSPANEKVSLVITDITGKVLMQENTSLTAGDNVKQIDVSRLSQGAYLIKAICNNGCE
                     TAVFKFTKY"
BASE COUNT         3268 a         1531 c         1431 g         3679 t
ORIGIN      
        1 ttaattatta cagatattat gatgccttat acttctggct tagaattagt aggcattgta
       61 aaagccaatt tgaataaaaa aacaccgatc attgttttat ctggtatggg agaagaaaat
      121 attgtattac aagcttttga aatgggtgct gacgattttt taactaaacc atttaaacct
      181 tctgaacttt ctttaagggt aaaaagatta gaaatgaaat taaggtaata tgtttcaaat
      241 aatttctaaa aaataatatt ttcttcttgg ttggtaatgt taaaacttta tttttgccgt
      301 ccttaataga atctgaaaga agattctgga cctatagctc agttggttag agcacctgac
      361 tcataatcag ggggtccctg gttcaagccc aggtgggtcc acaagtaaat caaaagccat
      421 ttaagcattt aacttaaatg gcttttgaaa tttaatagaa ctacttacag tatttgtatt
      481 tactgttatg aattaggaat ggtttttaat ttaaacttct aaaatctact ggtaccaatt
      541 tgctcacacc aggctcttgc atagttacac cataaattac atccaccgag cccattgttt
      601 gcttgttgtg tgttacaata ataaattgag aattatcgct gaactttttt atcatttgcg
      661 taaacttacc cacattagca tcatccaaag gtgcatctac ctcatctaaa atacagaatg
      721 gtgcaggttt aattaaataa attgcaaaca ataaagcagt agcagttaat gttttttctc
      781 ctccacttaa ttgtgtaata gaagatggac gtttgccttt aggttttgct acaatatcaa
      841 ccccagtttc tgctaagtta tctgggttta ctaaaatcat atccgcagta tcttcttctg
      901 taaacaatgc tttaaatacc ttttggaaat tttctcttac tttattaaag gtttctaaaa
      961 actgctgatt ggcggtggct tctacttctt gtatagtttg taataaacta tcttttgcct
     1021 gcactaaatc atttttttgt tctaaaataa attcataccg ttttttcatt tcagtaaatg
     1081 cttcaatagc agttgggttt atttcgccca tgttttctag ccttttttta agcttgtcgc
     1141 tatttgtctg aagagattca agtgtgtcat cagattttct tgcaccatct aaaatttcat
     1201 ctaattctac tttaaactca acattcattc tttctttcat gcctgccaag tttaatttca
     1261 gttcattcaa tttatcttta atagcactta ataaatattc aatattttct ttttctttta
     1321 ttttatgtct taattcactt tctctttctt gcaattgatt tcttaaaaca tggtaggttt
     1381 ggtctgcttc atttaccttc ttagcttctt cttctttagt gtgtaaaagc tgtaataact
     1441 cttcttcgtt tttaattagg ctgttttcgc cttctattat attatcttta gattcggtaa
     1501 gttgagtagt atttaattga atttgatttt gcaattcagt taactggttt tgtttaaaat
     1561 ctaactcttg ctttactgtt gcaattttac tttgttgttt agttaaatta atattgcttt
     1621 cgttaaataa aacagttgca gcattatatt gttgttctgc attttgatag tcttgctcaa
     1681 tcaactttaa ctcctctcct gtttgttgta ataattcagt taggtgctga aactcaaccc
     1741 tggtagttgc aatagaatta ttctcctcta ataacctatt ttcaatttct tctaactttt
     1801 gaaaagcatt ttgttgtgct atctgaatat tttctatttt attcttaatt gcaaaaacct
     1861 ggttagtaag ctgattaatt tcttgatcgg tttgcttaat tgtgttttct tttaactgtt
     1921 cattaaaccc aattactgca ttatggcgta tttgaatttc tgatttaaga gcattaacaa
     1981 ctgcttgttg caagattatt tcgtcttgaa gtttttctaa tttttttgct ctaccaattt
     2041 tattcccttc aaacaaacct atactaccac cagttaaagt aaattttcct ttaacatatt
     2101 ttccgctttt ttccagaatt acagcaccat ctgaatggtg aattacttca tcattttcgg
     2161 caataaaaac atttgccaat aaatgttgtg ctaaatgcaa atactgctca tctacttcaa
     2221 taacatctaa tgcttttatt gttaaggctg gttgatgaat gtcggactta acctcggcta
     2281 acttatccaa taaaaagaaa ttagctttgc cttttttatt agtatctaat aattgaacag
     2341 cttgtaaacc ttcttgtaaa ttgtttacta cataataatt aagatagggt tctaatacgt
     2401 tttctaaagc tgttctatat tcttctttta cataaattac atccgataat aatggtgctt
     2461 tatagttcca tgcttgatta ttgtgcaaaa acttcacgct atccggataa ccttccatcg
     2521 aatcaattaa actttttaaa agctcgtact catttttttt actatctaat tttctgttct
     2581 cttcagctaa ttcattcctt aaagactcaa gctgggcttg agtttcaaaa atttgctctt
     2641 tggttttatt gtattgttct tgtaattgtt ctaaatctaa ttttttcgtt tcaagtaaag
     2701 tttctttatc ttttaattca attaatagct gattttgttg agtttctctt tgttgttttt
     2761 cgtcttttaa ttgagtttga gttctttgca aattttgaat ggaagtatct gcaacagcta
     2821 cttttttttc agcatcaaaa cgtgttcttt gtacttgttg gtatttattt cttaattcat
     2881 ctaccaaaat tcttttccca tcaaatacac ttctgttggt atcaacttct gctcttgtgc
     2941 tagtaacttt ttctgttaaa tcatttagtt tatcactctc ttctttcaat tgtagctgtg
     3001 taaactcaat actatccttt gtatttttta attggccttc ggctttattt aaaaattcaa
     3061 ttaaactgct ttctttttct tttagatagt gtaatttttg agaagctaaa tttttgtcgt
     3121 tctctttggt tcttaatttt tgtaaaagct cgttaaactc atattgcatg ctttgtaaca
     3181 ctctctcttt ttcaatcatt ccaacttttt cttgttctaa agctgcatct tgtgttgcaa
     3241 tttgagcatc taattgtagt tttctgtttg tctctgattc ttgttgttca tttagctctt
     3301 tataggttaa attaaatcct tctaaagatg ctttggctaa ttcaattgct atttctttat
     3361 actcgttttt tatttcataa aatttctctg ctttttttgc ttggttttct aaagatttta
     3421 attgattgtt aatttcaaat aaaatatcct caattctaga caaatcttgt tctgtagcat
     3481 ctaatttatt ctttgcttct ttttttcgtg ttttgtagat agtaatgccg gctgcttgtt
     3541 ctaacatttt tctacggcta ttatctttat ctttaataat atcatctacc attcctaatt
     3601 caataattgc ataactatcg ttgctaacac ctgtatctaa aaataaatta tgtatatctt
     3661 ttaaacggca ttgcacatca tttaatctat attcactatc accattttta taaaatcttc
     3721 tagtaacagt aactgtagaa aactcggttg gtaaaaggtt ttttgtattt tcaaaagtca
     3781 agcttacttc tgctaaacca cttgcacttc ttgttttaga gccattaaaa accaaagctt
     3841 ctaaattctc acttcttaat gccgatattt tttgttcgcc aattacccat ctaatgctat
     3901 caattatatt gctttttccg cagccattag gtccaataat tcctgtaata ccttcatcaa
     3961 aacttaccac agttttatct gcaaaacttt taaacccttt tatttctaac gattttagtt
     4021 tcatgctact tcttcatttt ttaagtacct gcgaacataa ttgttttaag ctacttatta
     4081 aaacaccaat tatgcagtgt gtataaaagg tgagaaaaag tatttgtaat taataattat
     4141 tcaataaaca ataagtattt gattatgaat taaatactac gttactattc atttaaattt
     4201 tctatattaa ctctagtact atttattata atttcagaat ataaaataaa ataaatctca
     4261 aaatgtatta ataatcaatt tgatactaac caaataacaa ttataaaaag ttggcttgta
     4321 aatggcaaaa aattacatta taattgctat attaatgatt cataaaaaat ataatatgcc
     4381 atcctccaca ccaattaaac gaaacgaaat actgcaacta ataagtagag aacatcatta
     4441 tggtttatta cttagctgga aaattagaga aggaatgaaa aaaaatgttg aaataaacag
     4501 aataacggat tatgtaaact ggttttggga gagccattta atgcatcatt ttttgataga
     4561 agaaaaattt gttttttcaa ttttaccaac tgaaaacaat ttagtaattc aggcaattaa
     4621 tgaacacgaa aatttaagaa agttatttaa cgaacctgaa aaaaatgtag aaacactaaa
     4681 attaattgaa aaaaatttag accaacatat aaggtttgaa gaaagagttt tgtttaacga
     4741 agtgcaatta gttgccacca acgaacaatt agaattaatt gcattaaacc atacacatac
     4801 cttttctgat aactggaaag atgaattttg ggcaacagca aaataacaca acatctctat
     4861 ggttatattt ggaatagata atttattgcg ttcaaactgt agctggaaaa aagatagaat
     4921 tgctttggtt actaaccatg ctgctacaac aaaccaactg caaccaagta ggaaattgtt
     4981 attacaaaat ggttttaatt tggtaaaatt attttcgcca gaacatggct taaacttaat
     5041 gggagaagat ggggtaaaaa taaataatag caccgatgaa cttactaaat tgcccattat
     5101 tagtttgtat aaccaacaac taaaaccatt agaaaaagat attgatgata ttgatattat
     5161 tttgtttgat atacctgata taggctgtag attttatacc tatttgtgga cgatgactta
     5221 tattttagaa gctgcagcac aatttaataa accaataata actttagaca ggcctaaccc
     5281 aatttctggc aattttgaat tagcagaggg ttgcctttta gatgagaaaa actgttcttc
     5341 ctttattggt agatttagta ttccaattcg tcattgctgt accttggcag aacttgcaaa
     5401 ttattttaac aaagtaaaaa atattaatgc acaactagaa tgtattactt gttcaaattg
     5461 gagcagaaat atgtttcagc cagactggaa aataacattt gtgcctacat cgcctgcaat
     5521 acaaactttt aatgctgctt tgtattatac aggattaggc tttttagaag ctaccaatat
     5581 tagcgaagga agaggaacac aaaatgcatt tgaaatactt ggtgccccat ggttaaataa
     5641 tgaaatgtta tgtgaggtat ttaatagctt acaagatgaa gtagctctac aaccatataa
     5701 tttcacccca acatttagta aatataaaaa tgaaaaatgt tatggggtta gcttaaaagt
     5761 gaaaaatatt tcagtttatc atcctgttca tacagcatta ttgttaataa aaacaattaa
     5821 agaaactcat tctacatatt ttgcttggag tacttaccca acaatggtaa accaatctgg
     5881 agaaaaacat ttggataaat tactaggaat tttaaatagc gaaaatttat tagagttatc
     5941 ttttcaacca tttttaaaag agttaaataa aataacagat tcaaaggatt ggcagcataa
     6001 aataaatagt tttttattgt attaaaaatg tttactaaaa ttattaatat cttaaaactt
     6061 tattcaaaat tttaaaactg cttattcttg attatgtttg taccttataa aatttacaca
     6121 tgaaaaaaag catcttactt atagcactaa ttggatgtag ctttttatta aaagcacaac
     6181 aaaaatctta taaaatatcc atcattgcat tttataattt agagaatttt tatgacacca
     6241 tcaacgatcc aaataaaaat gatgaagaat ttacacccga aggaccaaga aattacaatg
     6301 gtgccattta ttgggataaa gttgggaaat tagcaactgt aatttcaaaa cttggtagcg
     6361 atgagaagcc agaaagtttg agccccgatg gtgcagctat tttaggtgtt gcagaggtag
     6421 aaaacgaaag tgttttaaaa gatttaacca atcatccatt acttaaaaaa agaaattatc
     6481 aaattgttca ttatgatagt aaggatgcaa gaggtgtaga tgttgcaatg ctctacaacc
     6541 ctaaatattt tattgttgaa gatagcaaac cactttttgt acaattacct ggtggtgcaa
     6601 aagatgccta ttttacaaga gatattctat gggtaaaagg taaacttgat ggcgaaacgg
     6661 tacatgttta tgttaatcac tggcctagca gattaggagg agaagaacgt agtgcaccag
     6721 ctagaaatgc tgctgcaatg gtttgtaaaa aacatattga tagtattgct gctgcagatg
     6781 gtgaacaaaa agtagttatc atgggcgact taaacgataa ccctaccaac gaaagtatta
     6841 cacaagtact taaagcaaaa gctaaaataa aagatgttag acctggtggt ttgtttaacc
     6901 cttggataaa tttatataaa aaaggttatg gcacattggc ctatcaagat gcttggggtt
     6961 tgtttgacca aataatgatt tcttacccat ggttaagtca agagcaaaat ggattttact
     7021 tttttaaaca agtaattttt aaccgagaat ttatgactga aaataaaggt cgttataaag
     7081 gttacccaat gcgtacttgg gatggtaata catacagagg tggttattgc gaccactttc
     7141 ctacgtatat tgttttactt aaaaaaatat aaaaaattat ctatttcaat tacatcaata
     7201 agcaaattgt tttgaaaaaa attattacta gtatcgttgt cttgtttata ttttcatgta
     7261 atgataaacc caaacaaaat gagcatgaaa atatagttca ggtgcaacaa ttgcaatata
     7321 gtgttcaaaa catataccct cataaccaaa cttcttttac cgagggatta gagtggcata
     7381 atggtttttt gtatgaaggc acaggcgata ctgaatatga gggaaacagt aaacttgcta
     7441 aaataaatct tcttacaggt aatgacattc aaaaaattaa cctatctaaa gagtattttg
     7501 gcgaaggtat taccttacta aataataaaa tttatcaact aacctataaa gaacaaaaat
     7561 gctttgtata tgatgccaaa acattaaaaa aaataaatga atttacttac gaaggcgaag
     7621 gctggggaat gaccaataat ggcaaagaaa ttattatggg taacggcagt aataatttat
     7681 atttcagaaa ccctgaaaac tttgcaatta ctaaaacatt aaatattaat aataattatg
     7741 ggccagtaac ggggctaaat gaattagaat atgtagatgg atttgtttat gcaaatgttt
     7801 ggccaaatta taaaattgta aaaataaatt taaacacttc taaagttgta gctgaagcta
     7861 gtttagagga tattttacat aagcactccc ctcaagacat agaaagaaaa gtagatgtat
     7921 taaatggaat tgcttatgat agtataggaa aaagattttt tattactggt aaatattggt
     7981 caaaggtttt tgaaatacaa tttaattagc tttacttaat acataaataa tagagctaca
     8041 ttgctctaca ttaaccaatg ttttaatatt tgaaatgcac ccatttagta ttgccattat
     8101 ataattattt ctcccattat ttttatactt ttcactaagc atactcacat aaaaactatc
     8161 aaaccacatt ggtttatatg cagcaattaa aaaattatgc tgctctgcta attttttaaa
     8221 acttaatggc gaaaaatggt ataaatgcct tggcacatca taagctgccc aatattcttt
     8281 ataaattgct gcatctgcac ttgtgtaatt tggcacagca ataaatactt ttccattttg
     8341 ttttaaaatt ttatttatgc attgcaaata accatgtaca tcatgtatat gttctaaaac
     8401 atgccataat gtaattgcat caaattgcaa aggctcttga tcaaaaagtg cttgtggatg
     8461 ttgaaatcga agtccatatt tttggaaggc aatattactg gctgttgtat ctggctctaa
     8521 cccttttacc tgccaaccag attgttgcat aatatttgca aatgcacctg tgcctgcacc
     8581 aatatctaac aaactaccat tggtaaaacc agtttgttct actattagtt tctttttagt
     8641 ttgtaaggta aaatttctaa ctgaatgata aattttattt attaatcctt ccttagtgtc
     8701 agaatgtgaa atataatttg ccgatttata gtaaggttca attgaatttg catctggtat
     8761 attttgtgta aaccttacac aacaatcatt acactgccaa acagcaaaaa tttgtttact
     8821 aacactaaaa tcatttgtaa caaatgcttc atttatattt tcactattac aacaaggaca
     8881 aaatgtttgg tgaataataa ctgacatgaa gcaaataaac taaagaatat tgtaaatggg
     8941 aaattgtttg tgcctttttt ctttttaaga attaggaata ttaaacataa tcaaaaaaat
     9001 tgagccacat taagtggctc aatttttttt aacaagaatg tgtatgcaat taatatttag
     9061 taaacttaaa tactgctgtt tcgcaaccat tgttgcatat tgcttttatt aaatatgctc
     9121 cctggcttag tctgctaaca tctatttgtt ttacattatc gcctgctgtt aaacttgtgt
     9181 tttcttgcat taatacctta cctgtaatat ctgttatcac taaacttact ttttcattag
     9241 ctggtgatac tatttgtaca ttaatggtat ttcttacagg gtttggataa acactagcaa
     9301 tacttatttg gtttacttta tcgcctttta agataactac agtagagtag ttgaacttgc
     9361 catctttatc tacttgtttt aaacgataat agtttgtacc tgctaatggt tttacatcgt
     9421 tatagccata gcttaaatta gatgtactgt taccattaat tgctttgctg tttacaaaac
     9481 ttagtttact aaattgttta ccatcgctac tacgttgtaa ttcaaaacca atattattac
     9541 tttctgtagc agtttgccat gataacatat ttaccatgcc ttctctttta cctgtaaaca
     9601 tggtgtaggt cactggtact gagcaatcta cagttacatt tacagtacca ttaacactgc
     9661 aattttctgc tgttgtggaa gatacagcgt aagttgttgt acttgcaggt ttagcataaa
     9721 ctgtatctaa acttgtacct gtataaggta tagttgcagc agcatctgta aataaacctg
     9781 ttaatggact ccaaacaaaa gttgttggta gtgaactgtt aagtataaag tctggtcttg
     9841 cgttaaaaga taaactaaca gtggtaccag atgctattgc agcaggtgtt ttgctatctg
     9901 ccctataca
//
LOCUS       DAOQPK010000067         9886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_3626_length_9886_cov_4.617638, whole genome shotgun sequence.
ACCESSION   DAOQPK010000067 DAOQPK010000000
VERSION     DAOQPK010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9886
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3626_length_9886_cov_4.617638"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="PLU36_04720"
     CDS             <1..970
                     /locus_tag="PLU36_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46084.1"
                     /translation="LLLTVDAKLKLENAFAKGETIGVLWQQLQPKTPRLNLLFQYPYI
                     FNSAFIADFNFNLFKKDTSFINIQSSIGFIYKPNYKQHIKFALQINQSNLLDIDTTTL
                     KLTKQLPDIIDVTINNIAIEYGYNSTDYKFNPRRGTEILLLSALGNKKIKKNNSISQL
                     KDGNFNFSNLYNNLKLNSYQIKIQTEASHYFSLSKYKILKTKINAGVIYSPTVYRNEM
                     YQIGGLKLLRGFDEESIFTDKYLVATTEYRYLFDVNAFFNCFIDYALSNNSIFNKSYQ
                     YLGLGGGLSFETKQGIFNIAIATGKRNDETINLKRTKIHIGFVSIF"
     gene            1045..2088
                     /locus_tag="PLU36_04725"
     CDS             1045..2088
                     /locus_tag="PLU36_04725"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HMZ46085.1"
                     /translation="MYKSKIGGIGMYVPTNIVTNKDLEKIMDTSDEWIQERTGIQERR
                     IAHRTNETTTTMGVEAAKIAIDRAGITAQDIDFIVFATLSPDYYFPGCGVLVQRALQM
                     KEIGALDIRNQCSGFIYALSVADQFIKTGMYKNILVIGSEKHSFGLDYTTRGRNVSVI
                     FGDGAGAVVLQQCNNESGILSTHLHSNGNDAETLAMYNPGTHANYWKKDLASFDEATI
                     GEMFMSHSMIDNAQNFPFMDGPAVFKKAVVKMPEVILEALQANNYKPSDIDMLIPHQA
                     NLRIIQFIQHSLKLADDKVYNNIQYYGNTTAASVPIALCEAWEKGKIKTGDLVCLTAF
                     GSGFTWASALIKW"
     gene            2142..2567
                     /locus_tag="PLU36_04730"
     CDS             2142..2567
                     /locus_tag="PLU36_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46086.1"
                     /translation="MKFIYTIIIFCVATTLHAQENEMPIQISAQNSTVKAYFKDNHKP
                     DTLLLKMNKKEEGKLVIMYANTKSENNLKRYFTITDKNNQPLKLFFLSRIVGITHILL
                     NDFFKQAQAGNFYNLYCTISTEDGKETKQFLLCTIKLNK"
     gene            2614..3516
                     /locus_tag="PLU36_04735"
     CDS             2614..3516
                     /locus_tag="PLU36_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HMZ46087.1"
                     /translation="MLRKIIYLINPISGTGKKEAIKKLIIAETEKKSIPFEILQTNAA
                     ANYSFLKEKIYQEKITDIVMVGGDGTINQVVNSLKELPIKFGIIPVGSGNGLARAAQI
                     PMKPKEALDLIFIGNSKKIDSFLVNNQFSCMLSGLGFDAKVAQDFSTKAGRGLLTYTT
                     QSLINFFKAQPYQFEISFENFSFYTDAFFISIANSNQFGNNVTIAPQASLSDGFLDIV
                     IVQKMNKAKLPFAILKQIRGNNKLQKLVDEVTNKNILYFQTPSLHIKNLKLAPFHIDG
                     EPKETFAEFEIDIIKDCFELIQPN"
     gene            complement(3687..4526)
                     /locus_tag="PLU36_04740"
     CDS             complement(3687..4526)
                     /locus_tag="PLU36_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HMZ46088.1"
                     /translation="MDNNQLKSLATQVRRDIVRMVHAVQSGHPGGSLGCTDFLTVLYF
                     KTMKYSTQFSMDGKNEDLFFLSNGHISPLFYSVLARAGFFEIKELATFRKINSRLQGH
                     PTTHEHLPGVRVASGSLGQGLSVAIGAAISKKLNNDNGLVFSLHGDGELQEGQIWEAA
                     MFAPHNKVDNLIATIDFNGQQIDGSTDKVLSLNNLKAKFEAFNWMVLEMNGNDIAEIV
                     NTLDVAKNNTGKGKPICILMKTTMGKGVDFMETGHEWHGIAPNDEQLAKALAQLPETV
                     GDY"
     gene            4829..5944
                     /locus_tag="PLU36_04745"
     CDS             4829..5944
                     /locus_tag="PLU36_04745"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HMZ46089.1"
                     /translation="MEQIILGLVLSFIVGFYAIPVIITVANQKKLYDIPNERKIHTTP
                     VSSLGGLGIFVAFMMGLLVATDSASSIKGFQYLIAAFMVIFFFGIKDDILILSPLKKF
                     IGQIIVACILVFKAELVITNMHGFLGIHAITHASGCLLTIFTIIVIMNAFNLIDGVDG
                     LAGTVSVITSVAFGFFNWVNGDIFYSVLAFSFAASVFSFLIYNYSPAKIFMGDTGAML
                     SGAVNAILVIHFIATAKQATAFPIEAAPAMGFGVLLMPLLDTLRVFALRMIHGRSPFY
                     PDRNHLHHFLLDRGFSHTQVTLTIAASALAFIFLTYFLLPIGVTWVILVQIVIFFTAV
                     YTLYLTRPNKAQVSKMRVIKVDFEEKEKTSTGTSVIN"
     gene            6069..6635
                     /gene="frr"
                     /locus_tag="PLU36_04750"
     CDS             6069..6635
                     /gene="frr"
                     /locus_tag="PLU36_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HMZ46090.1"
                     /translation="MLSEEIELVIDDTKSSMQKAINHLEQELLKIRAGKASPSMLDGI
                     MVDYYGNPTPINQAANISVLDAKTISIQPWEKSILAAIERAIMQANIGITPQNDGNQI
                     RLFLPPLTEERRKELFKKASGEGENTKVAIRNIRRDSIEQIKKLQKDGLSEDAAKDAE
                     AEIQTITDKFTALVGKHLEAKEKEMMSV"
     gene            complement(6632..7543)
                     /locus_tag="PLU36_04755"
     CDS             complement(6632..7543)
                     /locus_tag="PLU36_04755"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HMZ46091.1"
                     /translation="MTNKQKAYLALAVTSIVWGTTWVAVKFGVQKMPALQLAAIRLFF
                     AGLAFILFFTLVRKQSLPTKKQLQQLFFLSVFTFVLANALSAWSLQYIGSGLGALIGA
                     LYPLCVVIIEYFFYKNKSLNTTTLIGILLGFIGVGIVLYENTIDIHQEGFLIGILLSA
                     IATIAWSYSTIMIAKQKVKINPYYGLGWQMLFGSIMVLVLSLVTHNFIPISQIPIIAW
                     LMIAYLFIAGSIVAFIAFIYSMKHLSPTIASLYAYMNPIVAISIGTVLLDEKISIKLI
                     IGAIISLIGVYLVNYAAKKAMLKIDMD"
     gene            complement(7555..9075)
                     /gene="purH"
                     /locus_tag="PLU36_04760"
     CDS             complement(7555..9075)
                     /gene="purH"
                     /locus_tag="PLU36_04760"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HMZ46092.1"
                     /translation="MNKKIQTALISVFYKTGLETIAKKLHELGVTIYTTGGTQQFLND
                     INIPCIAVESVTNYPSILGGRVKTLHPLIFGGILGKRNDAQHLQDMQQYHIPIIDLVI
                     VDLYPFEETVANTTDENLIIEKIDIGGPSMIRAAAKNFNDVTVIASKNEYENFEKILT
                     NQQGEIFLEQRKAFAAKAFEIVMNYDIAIHNYFSPDNFILQSVTQKKILRYGENPHQE
                     AAFYGNLNNLFKQLNGKELSYNNLVDVDAAIQLMQEFKEVEESVFAIIKHTNVCGIAK
                     RKTVLESWNAALAGDNESAFGGILITNGTINKITAEAINELFFEVLIATAFEEEALQI
                     LRSKKNRILLEFNNKDTNQNNYQHKSLLNGVLKQQNDNHLIEEWKEVGGRTTTALEKE
                     DLAFANIISKHLKSNAIALVKNKQLVGKGCGQTSRIDALRQAINKAKQFNFSLKGAVL
                     ASDAFFPFNDCVSIAYKEGIEAFIQPGGSIRDKDSIEFAVQNNLAMVITSMRHFKH"
     gene            complement(9095..>9886)
                     /locus_tag="PLU36_04765"
     CDS             complement(9095..>9886)
                     /locus_tag="PLU36_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46093.1"
                     /translation="VPFSGLILYMEILLILINSFNTLSLILGNVIGYCINLMNKFILF
                     ADSLPFSVLPSIQISIVQACILFASIICFWIWDNSKKIKWMVIALSFLAAFFIVRCID
                     FINKSNQQKIIVYNVPNHTAIDLIEGRNYQFIGDTILTEDGFLRNFHIKPSRILHRVS
                     PNNQLPHFSIQNNIISCNGKSILIIDKRLETPLRNPKIMVDVIVLTNNPTIYLKDLHN
                     TFHCNQYVFDSSNPFWKIAYWKKDADSLHLQHHTTSTDGAFVMNL"
BASE COUNT         3544 a         1493 c         1454 g         3395 t
ORIGIN      
        1 tttactttta actgtagatg caaaattaaa attagaaaat gcttttgcaa aaggggaaac
       61 tattggagtt ttgtggcaac aattacagcc taaaacaccc aggttaaatt tattatttca
      121 gtatccttac atattcaatt cagcatttat tgccgatttt aattttaatc tttttaaaaa
      181 agatacttcc ttcataaata tacaatcttc tattgggttt atttataaac caaattataa
      241 gcaacatata aaatttgctt tgcaaataaa tcagtctaat ttattagata tagatacaac
      301 tactttaaaa ttaaccaagc aattgccaga tataattgat gtaactatta acaatattgc
      361 tattgaatat ggttataata gtacagatta caaattcaat ccacgaagag gaactgaaat
      421 tttattatta tctgctttag gcaataaaaa aataaaaaag aataatagta tatctcaatt
      481 aaaagatggc aattttaatt tttctaattt atacaacaac ttaaaattaa acagctatca
      541 aataaaaata caaacagaag catctcatta ttttagtttg agtaaataca aaattttaaa
      601 aacaaaaatt aatgcaggag ttatatatag ccctaccgtt tacagaaatg aaatgtacca
      661 aattggtggt ttaaaattgt taagaggctt tgatgaagaa agtattttta ctgataaata
      721 tttggttgcc acaaccgaat acagatatct ttttgatgta aatgcatttt ttaattgctt
      781 tattgattat gctttaagca acaactccat cttcaacaaa agctatcaat accttggctt
      841 aggtggtggc ttaagttttg aaactaaaca aggtattttt aatattgcaa tagcaacagg
      901 caaaagaaat gatgaaacta taaatttaaa acgaaccaaa atacacattg gttttgtaag
      961 tatattttaa tacaatatcg ttcatcaagt ttcattttta aatcttctta ttttagttta
     1021 ttttgccaaa ataaaaatag ctttatgtat aaaagtaaaa taggtggaat tggtatgtat
     1081 gtaccaacca atattgttac caataaagat ttagaaaaaa taatggatac aagcgatgag
     1141 tggattcaag aaagaacagg catacaagaa agaagaattg ctcatcgtac taatgagaca
     1201 accacaacta tgggtgtgga ggctgctaaa attgctatag atagagcagg aattactgct
     1261 caagatattg actttattgt ttttgcaaca ctaagccccg attattattt tcctggatgt
     1321 ggtgtattag ttcaaagagc tttgcaaatg aaagaaattg gtgcattaga tatacgaaac
     1381 caatgcagtg gttttattta tgctttaagt gttgccgacc agtttattaa aacaggtatg
     1441 tacaaaaaca ttttagtaat tggcagcgaa aaacacagct ttggattaga ttataccaca
     1501 agaggaagaa atgttagtgt aatttttggt gatggagcag gtgccgttgt actacaacaa
     1561 tgcaataatg aaagtggtat tttaagtacc catttacaca gcaatggtaa cgatgcagaa
     1621 accttagcca tgtataaccc tggcacacat gctaactact ggaaaaaaga tttagcaagt
     1681 tttgatgaag ctacgatagg agaaatgttt atgagccata gtatgataga caatgctcaa
     1741 aattttcctt ttatggatgg gccagcagta tttaaaaaag ccgttgttaa aatgccagaa
     1801 gttatcttag aagcattaca agcaaacaat tataaaccat cagatataga tatgttaatt
     1861 ccacatcagg ctaacttaag aataatacaa tttatacaac attctctaaa actggctgat
     1921 gacaaagtgt ataacaacat tcaatattat ggtaatacaa cagctgcaag tgtaccaatt
     1981 gcactctgcg aagcttggga gaagggtaaa attaaaacag gagatttagt ttgcttaact
     2041 gcgtttggta gtggatttac ttgggcaagt gcattaataa agtggtaatt ttattgctac
     2101 aaattcttac catattgttt cactcaataa aaaatttaaa aatgaaattc atttatacca
     2161 ttatcatatt ttgtgtagct actactttac atgcacaaga aaatgaaatg ccaattcaaa
     2221 tctctgcaca aaacagtaca gtaaaagctt attttaagga taaccataag cccgatactt
     2281 tattattaaa aatgaataaa aaagaagaag gcaaactggt aataatgtat gctaatacaa
     2341 aaagtgaaaa caatttaaaa cgttacttca caattactga taaaaataac caaccattaa
     2401 aattattttt tttaagtaga attgttggta taacacatat actgctaaac gactttttta
     2461 aacaagccca agcaggcaat ttttacaact tatattgcac cataagtaca gaagatggaa
     2521 aagaaacaaa acaattttta ttgtgtacaa taaaattgaa caagtaaaaa tgtgataaaa
     2581 aagttttttg ataactctaa attggaaaag gtgatgttgc ggaaaataat ttatttaatt
     2641 aatcccatat ctggcactgg taaaaaagag gcaataaaaa agttaattat tgcagaaaca
     2701 gaaaaaaaat caattccgtt tgaaatatta caaaccaatg cagctgctaa ttattctttt
     2761 ttaaaagaaa aaatatatca agaaaaaatt acagatatag taatggttgg cggagatgga
     2821 acaattaacc aagttgtaaa tagcttaaaa gaattgccta taaaatttgg aataataccc
     2881 gttggtagtg gtaatggttt agctagggca gcacaaatac caatgaaacc taaagaagca
     2941 cttgatttaa tatttattgg caattcaaaa aaaatagatt ctttcttagt caataatcag
     3001 ttttcttgta tgcttagcgg attaggtttt gatgcaaaag tagcacaaga ttttagtact
     3061 aaagcaggta ggggtttact tacctacacc acacaaagtt taattaactt ttttaaagcc
     3121 cagccctatc aatttgaaat tagtttcgaa aatttttctt tttataccga tgcatttttt
     3181 ataagtattg ccaatagtaa ccagtttggt aataatgtta ccattgcccc tcaagcaagt
     3241 ttaagcgatg gttttttaga tattgtaatt gttcaaaaaa tgaacaaagc aaaattgccc
     3301 tttgctattt taaaacaaat aaggggtaat aataaattac aaaagttggt tgatgaagtt
     3361 accaataaaa atatcttata ttttcaaaca ccctctttac atattaagaa tttaaagctt
     3421 gccccatttc atatagatgg tgaacctaaa gaaacttttg cagagtttga aatagatatt
     3481 attaaagatt gttttgaatt aattcagcct aattaattac agtagataaa tagaataaaa
     3541 agggtataaa aacaaaaaag gggccaggat aaatatccag ccccgttttt aaaatccaat
     3601 atcctatgaa aaaccgctgc taaggtaata cttattttag acaaaacatc accccaaaga
     3661 gatttttttt tttaaatttc ttaaaattaa taatctccta cggtttctgg caactgtgcc
     3721 aaagcttttg ccaactgctc atcattaggt gcaattccat gccactcatg gccagtttcc
     3781 ataaaatcca cccctttacc cattgtggtt ttcattaata tacaaatagg ttttccttta
     3841 cctgtattat ttttggctac atctagtgta ttaacaattt ctgcaatatc attaccattc
     3901 atttctaaaa ccatccaatt aaaggcttca aactttgctt ttaaattatt taagctcaat
     3961 actttatcgg ttgagccatc aatttgctgg ccattaaaat caatagtagc aattaaatta
     4021 tctactttat tatgaggtgc aaacatagca gcctcccaaa tttgcccttc ttgcaattca
     4081 ccatccccat gaagtgagaa gactagtcca ttatcattat ttaatttttt ggaaatagca
     4141 gcaccaattg caacgcttaa cccttgaccc aaagaaccac tagcaaccct tacacctggc
     4201 aaatgttcat gtgtagttgg gtggccttgt aatcttgaat ttatttttct aaaagttgcc
     4261 aattctttta tctcaaaaaa acctgctcta gctaaaaccg agtaaaacaa aggtgaaata
     4321 tgcccattgg ataagaaaaa caaatcttca ttttttccat ccattgaaaa ttgggtagaa
     4381 tatttcatgg ttttgaaata caatacagtt aagaaatcag tacagcctaa agagcctcct
     4441 ggatgtccac tttgtactgc atgtaccatt ctaacaatat ctcttcttac ttgagttgct
     4501 aaagatttta attgattatt atccatattt tttactttaa agcagcgaag ttaatttttt
     4561 ttggttctca ttaaatgtat tttataattg aaggttgcat ttcaagtttt cgcaacgttt
     4621 ttattattat aattactgat taaccaacca aacttttagt aataaaatat ttttagttgc
     4681 gaaaatttga tatgcataaa tgcttgtaat aaaacataaa gtataatttt tattgcattt
     4741 ttagggagaa aagctacatt tgcaattccc ctgctcataa taagctatga atatattaat
     4801 aaattcattt acgttttata attaataaat ggaacaaata atattaggtt tggtactttc
     4861 ctttattgtt gggttttatg caattccggt aataattact gtagctaatc aaaaaaagct
     4921 atacgatata cccaacgaaa gaaaaataca tacaactcca gtttcgtctt taggaggctt
     4981 aggtattttt gttgcattta tgatgggttt attggttgca accgatagtg cctcctccat
     5041 taaaggtttt caatatctta tagctgcctt tatggttatt ttcttttttg gaattaaaga
     5101 tgatattctt atcctttctc ctttaaaaaa attcattggt caaataattg ttgcttgtat
     5161 tcttgttttt aaagcagaat tagttattac caatatgcat ggctttttag gtattcatgc
     5221 aataacacat gctagtgggt gcttgcttac catttttact ataattgtta taatgaatgc
     5281 ctttaaccta attgatgggg ttgatggttt ggcaggaact gtaagtgtaa ttacttcggt
     5341 tgcttttggt tttttcaact gggtaaatgg cgatatcttt tatagtgtgc ttgctttttc
     5401 atttgctgca agtgttttta gttttttaat ttacaactac tctcctgcta aaatttttat
     5461 gggagatact ggagcaatgt tgagtggtgc tgtaaatgcc attttagtta ttcattttat
     5521 tgcaactgca aaacaagcaa ctgcttttcc tatagaggct gcaccagcaa tgggttttgg
     5581 agttttatta atgcctttat tagatacttt aagagttttt gctttaagaa tgatacatgg
     5641 tagatctcct ttttatcctg atagaaatca cttgcatcat tttttattag accgtggttt
     5701 tagccataca caagttactt taaccattgc agctagtgct ttagcattta ttttcttaac
     5761 ctatttttta ttacctattg gtgtaacttg ggtaatttta gtacaaattg ttatattctt
     5821 tactgccgtt tatacacttt atttaactag gccaaacaaa gctcaagtta gtaaaatgag
     5881 agttattaaa gtagattttg aagaaaaaga aaaaacttca accggcactt ctgttatcaa
     5941 ctaatattta ctaatttttt atttattatt ttcagttgta tatactgaaa ttgtttttgt
     6001 ataaagaaat aataacaaaa attgttcagt ttcattcatt agatttgcca ccaaattaaa
     6061 taaaagccat gttatcagaa gaaatagaat tagttattga tgatacaaaa tcatctatgc
     6121 aaaaagcaat taatcactta gaacaggagt tattgaaaat aagagctggc aaagcctccc
     6181 catccatgtt agatggaatc atggtagatt attatggaaa cccaacccca attaatcaag
     6241 ctgcaaatat ttcggtttta gatgcaaaaa caatcagcat tcaaccatgg gagaaaagta
     6301 ttttagctgc tatagaacgt gctattatgc aagccaatat tggtataaca ccacaaaatg
     6361 atggcaatca aattagatta tttttacctc cgctaaccga agaaagaaga aaagaattgt
     6421 ttaaaaaagc aagtggcgaa ggagaaaata ccaaagttgc tattagaaat attagaagag
     6481 attctattga gcaaattaag aaattacaaa aagatggctt aagtgaagat gctgctaaag
     6541 atgccgaagc tgaaatacaa actataactg ataaatttac agcattggta ggtaaacatt
     6601 tagaagcaaa agaaaaagaa atgatgagtg tttaatccat atcaattttt aacattgcct
     6661 ttttagctgc ataatttaca aggtaaaccc caattaatga aattattgca ccaataatta
     6721 gttttattga aattttttca tctaataaca cagttccaat cgatatagct acaattggat
     6781 tcatatatgc atatagagaa gcaatagtag ggcttaaatg cttcatactg taaatgaagg
     6841 caataaatgc aacaatagaa cctgcaataa acaaataagc aatcattaac caggcaataa
     6901 ttggaatttg gcttattgga ataaaattat gtgttactaa acttagcacc aataccatta
     6961 ttgaaccaaa aagcatttgc caacctaaac cataataagg attaattttt actttttgtt
     7021 ttgcaatcat tatagtgcta taactccatg caatggttgc tattgcagat aataaaattc
     7081 ctattaaaaa accttcttga tgaatatcta tggtattttc atataacaca atacctacgc
     7141 caataaagcc caataaaatg ccaattaatg tagttgtatt taatgattta tttttataaa
     7201 aaaagtattc aattataact acacataaag gatataatgc accaattaaa gcacccaatc
     7261 cgctaccaat gtattgtaaa ctccatgcac ttaaagcatt agctaacaca aaggtaaaaa
     7321 cagataaaaa aaataattgt tgtaattgtt ttttagttgg taaggattgc ttacgaacca
     7381 aagtaaaaaa taaaataaat gctaagccag caaaaaataa tctgattgct gcaagttgta
     7441 aggcaggcat tttttgaaca ccaaatttta ctgccaccca tgttgtaccc caaactattg
     7501 atgtaacagc taatgccaag tatgcttttt gtttattagt caaattaaag tttgttaatg
     7561 tttaaaatga cgcatacttg ttataaccat tgccagattg ttttgaaccg caaattcaat
     7621 actatcttta tctcttatac taccacctgg ttgtataaaa gcctctatac cttctttgta
     7681 agcaatactt acacaatcat taaaaggaaa aaatgcatcg ctagctagta ctgcaccttt
     7741 taaactaaaa ttaaattgtt tagctttatt aattgcttgc cttagtgcat caattcttga
     7801 tgtttgtcca cagcccttac ctactaattg tttatttttt accagtgcaa tggcattact
     7861 ttttaaatgc tttgaaataa tatttgcaaa tgctaaatcc tctttctcta atgcagtagt
     7921 tgttcttcca ccaacctctt tccattcttc tattaaatgg ttatcgtttt gttgttttaa
     7981 aactccgttt aataaagact tatgttggta attattttga ttggtgtctt tattattaaa
     8041 ctcaagtaat attctatttt ttttacttct taaaatttgt aaagcttctt cttcaaaggc
     8101 tgtggcaata agtacttcaa agaaaagttc atttatagct tcggctgtaa ttttgtttat
     8161 tgtaccattg gtaattaata taccaccaaa agcactttca ttatcgccag ccaaggcagc
     8221 attccaactc tcaagtactg tttttctttt tgcaatgcca caaacattgg tgtgtttaat
     8281 tattgcaaaa accgattcct ccacttcttt aaactcctgc attaattgta tagctgcatc
     8341 tacatcaacc aaattattat aagacagttc tttgccgttt aactgcttaa ataaattatt
     8401 taaattacca tagaaagcag cttcttgatg tgggttttcg ccgtaacgca atattttctt
     8461 ttgtgttact gattgtaaaa tgaaattgtc cggactaaaa taattatgta ttgctatatc
     8521 ataattcata acaatttcaa aagcttttgc agcaaaagct tttctttgtt ctaagaaaat
     8581 ttcaccttgt tgattggtta aaattttttc aaaattttca tattcgtttt tagaggctat
     8641 tactgttaca tcattaaaat ttttagctgc agctcttatc atgcttggcc caccaatatc
     8701 tattttttca ataattaaat tttcatctgt tgtattggct actgtttcct caaaaggata
     8761 taaatcaaca attaccaaat caataattgg tatatggtac tgttgcatat cttgcaaatg
     8821 ctgagcatca tttctttttc ctaaaatgcc accaaaaatt agagggtgta aagtctttac
     8881 tctgcctcct aagatagatg gataattggt tacactttct actgcaatac aaggtatatt
     8941 aatatcattt aaaaattgtt gggtgccgcc agtggtataa atagttactc ctaactcatg
     9001 cagctttttt gcaatagttt ctaaaccggt tttataaaaa acagaaataa gtgctgtctg
     9061 aatttttttg ttcataaatt aagttatttc aaaatcaaag attcataaca aaagccccat
     9121 cagttgaagt tgtatgatgc tgcaaatgta aactgtctgc atcttttttc cagtaagcaa
     9181 ttttccagaa tgggttagaa gaatcaaaaa catattgatt acagtggaaa gtgttgtgca
     9241 aatcttttaa atatatggtg ggattattgg ttaatacaat tacatctacc ataatttttg
     9301 ggtttcttaa tggagtttcc aatcttttat caataataag aatggattta ccattacaac
     9361 taattatatt attttgaata gagaaatgtg gaagttggtt atttggagaa accctgtgta
     9421 atattctaga aggttttata tgaaagttcc ttaaaaatcc gtcttccgtt aaaatagtat
     9481 caccaatgaa ttgataattt cttccttcta ttaaatcaat agcagtatgg ttgggtacat
     9541 tatatacaat tattttttgt tgattagatt tattaataaa atctatacaa cgaacaataa
     9601 agaaagcagc aagaaaagat aatgcaataa ccatccattt tattttttta ctattatccc
     9661 aaatccaaaa gcaaatgata gatgcaaata aaatacaagc ttgaacaata cttatttgaa
     9721 tggaagggag aacagaaaaa ggtaaactat cggcaaacaa aataaatttg ttcattaaat
     9781 taatacagta gccaattaca ttgcctaaaa ttaagctaag agtgttaaat gaattaatta
     9841 gaataagcaa aatttccata tataatatta aaccagaaaa tggaac
//
LOCUS       DAOQPK010000068         9826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_3659_length_9826_cov_4.832770, whole genome shotgun sequence.
ACCESSION   DAOQPK010000068 DAOQPK010000000
VERSION     DAOQPK010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9826
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3659_length_9826_cov_4.832770"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /locus_tag="PLU36_04770"
     CDS             <1..1320
                     /locus_tag="PLU36_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46094.1"
                     /translation="LGSLVVFSGSSPNQANKETTDKNGKSVGSLSYAFSKAITNLPIN
                     CNYNLLFEKVKATVQADEPTQIPMFEGNGTLKVFSNQFIPLKDIIAVEQRFNNAANNF
                     NDTTFVIQRGQFNNINTGTELNVYVMGTTELYCKAFVQQANNFQSICIATKKLSNKNT
                     YEVKVESLVYGAVTASYNIQNKIPTATNAIEKQLQHFLQQQPFLLKNNNPDYTLNISQ
                     ENNSTYIQLIEKNDSVRYSSKFSTNDSLSANNLEDLLESIKKGMRVKYLRKMEDGGSL
                     YNNVQVTIHPLQKAVSDNELIFYPNDSFQLKIKSNYDGILYFTVLDLLPDNNIKILIP
                     DTSKLANDADYSLRKNEERQFTIYTDETSITGKEFLKVIFSPYPIDLRPSFQLYSTRT
                     INTKLKPIEKAMDDLFPKNKNTLTRSTPVKMDDIAIKTVGFTLRKSK"
     gene            1336..1809
                     /locus_tag="PLU36_04775"
     CDS             1336..1809
                     /locus_tag="PLU36_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HMZ46095.1"
                     /translation="MKIQIWSIGKQHESYIKEGVEDFTNRLTKYFTTEWKIIAPPKNN
                     AALNEKDLKKEEAGIILPLIQKADFLVLLDERGKQLSSIQLANFIELQANQSTKKIIF
                     LIGGAFGVDDTVFKRADTVLSLSQFVFPHMLVRLIIAEQLYRACTIIKNERYHHK"
     gene            1857..2492
                     /locus_tag="PLU36_04780"
     CDS             1857..2492
                     /locus_tag="PLU36_04780"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HMZ46096.1"
                     /translation="MSFTLIIIIVTAIISFSAFKNEKIVNDLIFYPPSITNQNQWYRF
                     ITCGFIHADVVHLAFNMLSLYMFGEAVENNFKYIFGTRGNVLYVVLYFSTLVICLLPT
                     YLKQKQNSYYRSLGASGAVSAIVFVGIFLFPTAKIGIFILPPIIPGFIFAPIYLYVSW
                     HLAKKGEDNINHSAHIWGAVYGLIFFIGACYILSSFDPVNNFIQSILEWAK"
     gene            complement(2576..4420)
                     /gene="ftsZ"
                     /locus_tag="PLU36_04785"
     CDS             complement(2576..4420)
                     /gene="ftsZ"
                     /locus_tag="PLU36_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HMZ46097.1"
                     /translation="MIHFDLPKQKSSIIKVLGVGGGGSNAVNFMFSQNVEGVDFIICN
                     TDAKAIEQSKVPNKIQLGPFLTQGLGAGANPEVGKAATEESLEEIKRILEVNTKMAFI
                     TVGMGGGTGTGGAPIIAKICKELGILTVGIVTTPFGFEGPRRMHQAEDGIKQLKPFVD
                     TLLVISNDKLRMQYGNLKMGEAFSKADNILSTAAKCITDIINSRGHIIVDFADVCTVM
                     KNGGVAILGKAEVEGENRAQLAIEEALNSPLLNDSDIKGAKWILININSAEGEHECTM
                     DELEIINNYLRMQAGEHTDVIVGTGYDNNLGGKIGITLVATGFDAKDPFAKPVEVKKE
                     MPKPEKIILTLDSDVEEKKEEVKPFIQQELPLVDKDPYAPRLLESQDELQNNNFQDVS
                     TSTPHNVELLSSVEEESPILHYELSTEITEEVNITIPSVIDEQTTSTTEENNEIETNR
                     FLTKPSNIYAEPDYEKPSSMSGHLMEPNLTTEQNEELPELEMKLVERDVNPVAADDAT
                     VKAQTEIPANLSAEEPVLLDDVEEQKRRAVERLQKLRNLSFNVNNTSDPNNEFDSVPA
                     YIRRNMELFGNTLTSVENFYSKYTVNKDENDQTQISTINTFLDGKKPD"
     gene            complement(4483..5871)
                     /gene="ftsA"
                     /locus_tag="PLU36_04790"
     CDS             complement(4483..5871)
                     /gene="ftsA"
                     /locus_tag="PLU36_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HMZ46098.1"
                     /translation="MNHNEAPIIVGLDIGTTKIAAIAGRKNEFGKLEILGFGKANSNG
                     VKHGQVLNIDQTIKSIQQALQNCLASNPDLEIKEVYVGIAGHHIKSLQTRGDIVRQQP
                     DDEIQGSEIDVLIDNQRKTFIPAGDQIIDVIPQEYYVDNIQNIKDPVGYNGVKVGANF
                     HIITGDKNAIKNINRAVNKSGLQTIDLVLQPLASSSAVMSDIDFEAGVVVLDIGGGTS
                     DLAIYYEGVLKHTAVIPFGGENITEDIRMGLGVLRQQAEALKVQFGSALVDDKKANSY
                     ITIPGIKGMPAKEIGVNNLSQIIMARMGEILDFVSYHLKQVGLDSKMLNGGIIITGGG
                     SQLKHLIQLTEYTTGLNARIGLPNEHLAAGHIEELKKPMYATCLGLILKGYNDHEHKY
                     KIFSESNPGISVPTSLKSDKEELEKNSKPEEPKSVDVEERAVKLKFWDKIGSKVKRNL
                     IELFKEEEDKTI"
     gene            complement(5908..6771)
                     /locus_tag="PLU36_04795"
     CDS             complement(5908..6771)
                     /locus_tag="PLU36_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46099.1"
                     /translation="MIKKKLILLMWIAVGILTVVLLGAAMQKKNEYKCKDIKIEIVGS
                     VQHMFVDEKDILQTVNSIGLVTGNSISNINLRNIELLLEKNAWIKNAEVYFNNNNELI
                     INIEEKEPVARIFTLQESSFYVDTLANQLPISDKYSARVPVFTAFPTDHQQLSMADSL
                     LMVDVIKLAKFIQADSFWNAQIAQVNILPNATFEIIPVLGNQIISLGNADELDKKFKR
                     LLAFYKYAWLQNGINKYERLNVEFNNQVVAIKKGVSVFSDTTKAKQIIITVDSVKAET
                     INSPKAVFKKN"
     gene            complement(6772..8127)
                     /gene="murC"
                     /locus_tag="PLU36_04800"
     CDS             complement(6772..8127)
                     /gene="murC"
                     /locus_tag="PLU36_04800"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HMZ46100.1"
                     /translation="MIELDNIKKIYFIGIGGIGMSALARYFKFKKVEVAGYDKTKTAL
                     TQLLENTGINIHYSEDVDAIPKDVDVVVYTPAIPNEHKELVYYQQHQYHVVKRSDILQ
                     CITESSFNICIAGTHGKTTVTTMIAHVLRHSGFGCNAFIGGIASNYNTNFWSSENNVV
                     VVEADEYDRSFLKLVPDVAVITAMDADHLDIYGTAQELEKTFIQFSKKLKQNGCLITK
                     HGLKRDTELNADTKFTYSFQNNLASVYAGNIRVVNGSYVFDVIGNQFNINNITLHLGG
                     LHNIENAIAAITVATYLKIDDSKIKDAIEDFKGVKRRFEYIVRNEENVLIDDYAHHPE
                     ELKALINGVRSIYKNEKLTIVFQPHLYSRTNDLAAGFAESLDLADEVILLPIYPAREL
                     PIAGVESEMILSKMNLKNKQVLSKNDFINWVKNNKPKMLVMAGAGDIDVMVNDVKEIL
                     A"
     gene            complement(8124..9221)
                     /gene="murG"
                     /locus_tag="PLU36_04805"
     CDS             complement(8124..9221)
                     /gene="murG"
                     /locus_tag="PLU36_04805"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HMZ46101.1"
                     /translation="MIEATKIIIAGGGTGGHIFPAIAIANALKLHQPNIQILFVGAKG
                     KMEMEKIPQAGYEIKGLDIAGFNRSSLIKNIGLPFKLIKSFIQVRSIFKQFKPHAVVG
                     VGGYSTFPVLKYAQAKSIPTFIHESNSFAGKANRMLGKNATKIFVATSGMEQFFTKDK
                     IFISGNPVRKSIVENSITKQEALKFFNLREEKKTVLVIGGSLGAKSINQAIAMQINEF
                     AKNNLQLIWQTGKLTADEYLAIAKGKSNIWCNHFINTMEMAYAAADIVISRAGAMAVA
                     ELCISAKPVVFVPFPFAAEDHQTANAKVLVNKQAALMIQDNEVKEKLVETVFELAKND
                     EKMQSMRTNIASLAIKNADEIIASEILKNIK"
     gene            complement(9322..>9826)
                     /locus_tag="PLU36_04810"
     CDS             complement(9322..>9826)
                     /locus_tag="PLU36_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HMZ46102.1"
                     /translation="DTEVPYQVQQAKIAIAKGGILIGQGPGNSEQRNFLPQAYNDFIY
                     SIIIEEYGLLGAAVIIFIYLSFLFRCIKIFRRCPYAFGAFLALALSFTLVIQAIANMA
                     VNVNLLPVTGVTLPLVSMGGSSFLFTCAAIGIILSVARNVEQLEGVKDEQDRTNNFNG
                     QQQAVVE"
BASE COUNT         3128 a         1744 c         1305 g         3649 t
ORIGIN      
        1 ttaggaagcc tggtagtatt tagtggaagt agccctaacc aggcaaataa agaaacaacc
       61 gacaaaaatg gaaaaagtgt tgggtctttg tcttatgcat tttctaaagc tattactaac
      121 ttgccaatta actgtaatta taatttatta tttgaaaaag taaaagcaac agtacaagca
      181 gatgagccaa ctcaaatacc catgtttgaa ggtaacggaa ccttaaaagt attcagtaat
      241 caattcattc ctttaaaaga tataatagca gtggagcaac gttttaacaa tgcagcaaat
      301 aattttaacg atactacttt tgttattcaa agaggccaat tcaataacat taataccgga
      361 acagagctaa atgtttatgt tatgggcact acagaattgt attgtaaagc atttgtacaa
      421 caagccaata attttcaaag tatatgtatt gctactaaaa aattatctaa taaaaatact
      481 tacgaagtaa aagttgaatc tctagtatat ggtgctgtta cagcatctta caatattcaa
      541 aataaaatac caactgctac aaatgcaata gaaaaacaat tgcaacattt tttgcaacag
      601 caaccttttt tattaaaaaa taataatcca gattatacct taaacatttc tcaggaaaac
      661 aattcaacct acattcaatt aattgagaag aatgatagtg ttaggtactc ttcaaaattt
      721 tctacaaatg attccttaag tgcaaacaat ttagaagact tgttagaaag tataaaaaaa
      781 ggcatgagag taaaatattt acgaaaaatg gaagatggtg gaagccttta taataatgta
      841 caagtaacca tacatccatt gcaaaaagcc gttagcgata atgaattaat tttttatcca
      901 aatgatagtt ttcaattaaa aattaaaagc aattacgatg gtattttata ttttactgta
      961 ttagatttat tgccagataa taatataaaa attttaatac ccgatacttc taaacttgct
     1021 aatgatgcag attactcttt aagaaaaaat gaagaaaggc agtttactat atataccgat
     1081 gaaacgagta ttactggtaa agaattttta aaagttattt tctctcccta tcctattgat
     1141 ttaaggccat cttttcaact gtattcaaca agaacaataa acacaaaact aaaaccaata
     1201 gaaaaagcta tggatgattt atttccgaaa aataaaaata cattaaccag aagtacccca
     1261 gttaaaatgg atgatattgc aattaaaaca gttggtttta ctttaagaaa aagcaaataa
     1321 aattatttcc aaaaaatgaa aattcaaatt tggagcatag gcaagcagca tgaaagttat
     1381 ataaaagagg gtgttgaaga tttcactaac aggcttacta aatattttac aacagaatgg
     1441 aaaattattg ccccaccaaa aaacaatgca gcattaaatg aaaaggatct aaaaaaagaa
     1501 gaagctggaa taattttacc cttaatacaa aaagcagatt ttttggtttt attagacgaa
     1561 aggggcaaac aattatcctc tattcaatta gctaacttta tagagctgca agcaaaccaa
     1621 agtacaaaaa aaatcatatt cttaattggt ggtgcatttg gcgttgacga tacagttttt
     1681 aaaagagctg ataccgtttt aagtttatct caatttgttt ttcctcacat gctagtaaga
     1741 ttaattattg cagagcagtt gtatagggct tgtactatta ttaaaaacga aaggtatcat
     1801 cacaaataat tttttttatt tattttatta gattctcaat taattaattt tgtataatga
     1861 gttttacctt aattataatt atagtaacag ctattatttc attctctgca tttaaaaacg
     1921 aaaaaatagt taacgattta attttttatc caccctccat taccaaccaa aatcaatggt
     1981 atagatttat tacttgtggc tttattcatg cagatgttgt acatcttgct tttaatatgt
     2041 tatcgttata tatgtttgga gaggcggttg agaataattt taaatatatt tttggtacta
     2101 gaggaaatgt tctttatgta gtgttatatt ttagtacatt agtaatatgt ttattgccaa
     2161 cttatttaaa acaaaaacaa aatagttatt atagaagttt aggagcatct ggtgctgtat
     2221 ctgccattgt ttttgttggt atttttttat ttccaactgc aaaaattgga atatttattt
     2281 tacctccaat tattccaggg ttcatttttg caccaatata tttatatgtt tcctggcatt
     2341 tagccaaaaa gggcgaagat aatattaacc actctgctca tatttggggt gctgtatatg
     2401 gattaatatt ttttatagga gcttgttata ttttaagcag ttttgatcct gtaaataatt
     2461 ttattcagtc tatacttgag tgggcaaagt agttaaaaaa ataatcgctt ctttgcagaa
     2521 gcgattatca caacaaaaaa cacattcaaa agaaaatctc taaaagccaa cctatttaat
     2581 ctggtttttt tccatctaaa aaagtattta tagtagaaat ttgagtttga tcattttcat
     2641 ctttatttac agtgtacttg ctgtaaaagt tttcaaccga tgtaagcgta tttccaaata
     2701 attccatgtt gcggcgaatg taagcaggaa cagaatcaaa ctcattgtta ggatctgagg
     2761 tattgttaac attgaaagac agattacgta atttctgtag cctttctacc gccctgcgtt
     2821 tttgctcttc tacatcgtcc agtaaaactg gttcctctgc agacaaatta gcaggtattt
     2881 ccgtttgagc tttaactgtt gcatcatccg ccgccactgg atttacatct ctttctacca
     2941 acttcatttc gagctccggt agctcttcat tttgttcagt agtaagatta ggttccataa
     3001 gatgtccgga catagacgat ggcttctcat agtcaggctc tgcatagata ttagatggtt
     3061 ttgtaagaaa cctattggtt tcaatttcat tattttcttc agtagttgat gtagtttgtt
     3121 catctataac tgaaggtata gttatattta cttcctctgt tatttcagta cttaactcat
     3181 agtgcaaaat agggctttct tcttctactg aactaagtaa ttctacgttg tgtggagtac
     3241 ttgtggaaac atcctgaaaa ttattgtttt gtaattcatc ttgtgattct aataatcttg
     3301 gtgcataagg atctttatct actaatggca actcttgttg aatgaaaggt ttcacttctt
     3361 cttttttctc ttcaacatcg ctatctaatg ttaagataat tttttcaggt tttggcattt
     3421 ctttttttac ttctacaggt tttgcaaatg ggtcttttgc atcaaaacca gtagccacta
     3481 atgttatacc aattttaccc cctaaattat tatcatatcc tgtaccaaca ataacatctg
     3541 tatgttctcc tgcttgcata cgcaagtaat tatttataat ctctaattca tccattgtac
     3601 actcatgttc accttcggca ctatttatat ttattaatat ccatttagct ccttttatat
     3661 cactatcatt caataatgga gagttgagtg cttcttcaat tgcaagttgg gctctgtttt
     3721 ctccttctac ttctgcctta cctaaaatag ctactccacc atttttcatt acagtacaaa
     3781 catctgcaaa gtcaacaata atatgtccac ggctattgat aatatctgtg atacattttg
     3841 cagcagtaga taaaatatta tctgctttag aaaatgcttc tcccattttt aaattaccat
     3901 attgcattct taatttatcg ttgctaatta ctaataaagt atcaacaaag ggtttcaatt
     3961 gtttaatacc atcttctgct tggtgcatac gtcttggacc ttcaaaacca aaaggtgtag
     4021 ttactattcc tactgttaaa atacctagtt ctttacaaat ttttgcaata attggtgcac
     4081 caccagttcc tgttcctcca cccataccaa cagtaataaa agccattttg gtatttactt
     4141 ctaaaatgcg ttttatttct tctaagcttt cttctgttgc tgctttacca acttctggat
     4201 tggcaccagc acccaaacct tgtgttaaaa atgggcctaa ttgaatttta ttaggaactt
     4261 tactttgttc aattgcttta gcatcggtat tgcaaataat aaaatctacg ccttctacat
     4321 tttgagaaaa cataaagttt acagcattgc ttcctccgcc acctacacct aaaactttaa
     4381 taatagatga tttttgtttt ggcaaatcaa agtgtatcat agttattaat tttaatgggt
     4441 taatttttat ttttattggt tagtaattgt tagaatttaa tattaaatag ttttatcctc
     4501 ttcttcttta aacagctcaa ttaggtttct tttaactttg ctgcctatct tatcccagaa
     4561 ctttaattta actgcacgtt cttccacatc tactgatttg ggttcttctg gtttactatt
     4621 tttttctagt tcctctttat ctgattttaa actagtaggt acagaaatac caggattact
     4681 ttctgaaaat attttgtatt tgtgttcgtg atcgttatag ccttttaata ttaaacccaa
     4741 acaagttgca tacattggct ttttaagttc ttctatatgg cctgcagcaa gatgctcatt
     4801 tggtaaccca attcttgcat ttaaacctgt ggtatattct gttagttgta ttaggtgttt
     4861 taattgagag cctccaccag taattattat tcctccattt agcattttgc tatctaagcc
     4921 tacttgtttt aaatgatagc taacaaaatc taaaatttct cccattcttg ccataataat
     4981 ttgagataaa ttatttaccc caatttcttt agctggcatc ccttttattc ctggtattgt
     5041 tatataggaa ttagcttttt tatcatctac taatgcacta ccaaattgaa cttttaaagc
     5101 ttctgcctgc tgacgtaaaa cgcctaaccc cattcttata tcttctgtaa tattttcgcc
     5161 tccaaaagga atgactgcag tatgttttaa aacaccttca taataaattg ccaaatcgct
     5221 tgttcctcca ccaatatcta gcactacaac acctgcctca aaatcaatat cactcattac
     5281 agcactagaa cttgcaagag gttgcaatac aagatctata gtttgcaaac cacttttatt
     5341 cacagcccta ttaatatttt taattgcatt tttatcccct gttatgatat gaaaatttgc
     5401 acccactttt acaccattat acccaacagg atctttaata ttttgaatat tatctacata
     5461 atattcttga ggaataacat caataatttg atctcctgca ggaataaatg ttttacgttg
     5521 attgtctatc agcacatcaa tttcagatcc ttgaatttca tcatctggtt gctgtcttac
     5581 tatatctcca cgggtttgta aacttttaat atgatggcca gctatgccaa catatacttc
     5641 tttaatttct aaatctggat tactagctaa acaattttgc aatgcttgtt gtatagactt
     5701 tatggtttga tctatattta aaacctggcc atgttttacg ccattactat tggccttgcc
     5761 aaagcctaag atttcaagtt ttccaaactc attttttcta ccagcaattg ctgcaatttt
     5821 tgtagttcca atatcgagcc caacaataat gggtgcttca ttgtgattca tagatatgtg
     5881 ttttgttgtt gtttataata ttaattttta attttttttg aatactgctt taggtgaatt
     5941 aatagtttct gcctttactg aatctactgt tattattatt tgtttggctt ttgtagtatc
     6001 tgaaaaaaca ctaacccctt ttttaatagc tactacttga ttattaaatt caacatttaa
     6061 tctctcatac ttgtttattc cattttgcaa ccaagcatat ttataaaaag ccagcagtct
     6121 tttaaatttt ttatccaatt catctgcatt acctaaactt attatttggt tacccaacac
     6181 aggaataatt tcaaatgttg cattgggtaa aatatttact tgtgcaattt gagcattcca
     6241 aaaactatct gcctgtataa attttgccaa ctttattaca tctaccatta acaagctatc
     6301 tgccatggaa agttgttgat gatctgttgg gaatgccgta aatacaggaa ctctagcact
     6361 atatttatcg ctaattggca actggtttgc tagtgtatca acataaaaag aactttcttg
     6421 taaggtaaaa attcttgcta ctggttcttt ctcctctatg tttataatta actcattatt
     6481 gttgttaaaa taaacttccg cattttttat ccaagcattt ttttctaata acaattcaat
     6541 atttcttaaa ttaatattac taattgaatt gcctgttact aaaccaattg aattaactgt
     6601 ttgtagaata tctttttcgt caacaaacat gtgctgtact gaaccaacaa tttcaatttt
     6661 gatatccttg cacttgtact cattcttttt ttgcatggct gcaccaagta atactacagt
     6721 tagtatgcct actgcaatcc acattaataa tatgagtttc ttttttatca cttatgctaa
     6781 aatttctttt acatcattta ccattacatc aatatctcct gcacctgcca ttaccaacat
     6841 ttttggctta ttatttttca cccagtttat aaaatcattt tttgataaca cttgtttatt
     6901 tttcaaattc attttgctca aaatcatttc actttcaacc cctgcaattg gcaattctct
     6961 agctggataa atgggtaata atattacttc atctgccagg tctaagcttt ctgcaaaacc
     7021 tgctgccaaa tcatttgttc tgctatataa atgaggttga aatactatag ttaatttttc
     7081 atttttataa atacttctaa ctccatttat caatgccttt aattcttctg gatgatgagc
     7141 ataatcatca attaaaacat tctcttcatt tctaactata tattcaaatc ttcttttaac
     7201 tcctttaaaa tcttcaattg catcttttat tttactatca tcaatcttta aatatgtagc
     7261 aactgtaatt gctgcaattg cattttctat attatgcaag ccacccaaat gaagtgttat
     7321 attattaata ttaaattgat tgcctataac atcaaaaaca taactaccat ttactactct
     7381 aatattacct gcatatacac ttgctaaatt gttttgaaag ctgtatgtaa attttgtatc
     7441 agcattcaac tcagtatctc tttttaaccc atgtttagtt attaagcagc cattctgttt
     7501 taactttttt gaaaactgaa taaatgtttt ttccaactct tgggcagttc cataaatatc
     7561 taaatggtct gcatccatag cagtgataac tgcaacatct ggtaccagtt ttaaaaaact
     7621 cctatcatat tcatctgcct ctaccaccac tacattattt tcactactcc aaaagttggt
     7681 attgtaattg cttgcaatac ctccaataaa agcattgcat ccaaaaccgc tatgtcttaa
     7741 aacatgagca atcatagtag ttacagttgt tttaccatgc gtgcctgcaa tacatatatt
     7801 aaaagaactt tcagtgatac attgtaaaat atcgctacgt tttacaacat ggtattggtg
     7861 ttgttgataa tacactaatt ctttatgctc gtttggaatt gcaggtgtat aaaccactac
     7921 atccacatct ttgggaattg catctacatc ttcggagtaa tgaatgttta tgcctgtatt
     7981 ttcaagaagt tgggttagtg cagtttttgt tttatcatac cctgctactt caaccttttt
     8041 aaatttaaaa taccttgcta aagcactcat accaatgcct ccaattccaa taaaataaat
     8101 tttctttatg ttatctaatt ctatcattta atatttttca aaatttctga tgcaataatt
     8161 tcatccgcat ttttaattgc taacgatgca atattggtac gcatagattg catcttttca
     8221 tcattttttg ctaattcaaa aacagtttct actaattttt ctttaacctc attatcctgt
     8281 atcattaatg ctgcctgttt attcactaaa acttttgcat ttgcagtttg atgatcttct
     8341 gcagcaaaag gaaatggcac aaacactaca ggctttgcag aaatacataa ttctgcaact
     8401 gccatagctc ctgcacggct aattacaata tctgctgctg cataagccat ttccattgta
     8461 ttaataaaat ggttacacca aatattgctt ttaccttttg caatagctaa atactcatcg
     8521 gctgttaact ttccggtttg ccaaataagc tgcaaattat tttttgcaaa ctcgtttatt
     8581 tgcatagcaa tagcttgatt aatgcttttt gcacccaagc tgccaccaat taccaatacc
     8641 gtttttttct cttctcttaa attaaaaaac tttaatgcct cttgtttagt tattgaattt
     8701 tctacaattg attttcgtac aggattacca gaaataaaaa ttttatcttt tgtaaaaaat
     8761 tgttccatgc ccgaagtagc cacaaaaatt tttgtagcat ttttacccag cattctattt
     8821 gctttaccag caaaagagtt actttcatga ataaatgtag gtattgattt tgcttgggca
     8881 tatttaagta ctggaaatgt tgaataacca ccaacgccaa ctactgcatg tggcttaaac
     8941 tgtttaaata tgctgcgtac ctgtataaaa ctttttataa gtttaaaagg caaaccaata
     9001 ttttttatca aagaacttct attaaaacca gctatatcta atccttttat ttcatatcct
     9061 gcctgaggaa ttttttccat ttccattttc ccttttgcac caacaaacaa aatttgaatg
     9121 tttggttgat ggagctttag tgcatttgca atagcaattg caggaaaaat atgacctcct
     9181 gttcctccac cagctataat aattttagta gcttctatca tagttggcta gttatatttt
     9241 tcattttgtt ccactttcta aaaaaattat ttctttcttc tttcatttct aaaccaatcc
     9301 acatctttaa ccaattactt cttattcaac cacagcttgc tgttggccat taaaattatt
     9361 tgttctgtct tgttcatctt ttactccttc taattgttct acatttctag caacacttaa
     9421 tataatacca attgcagcac atgtaaataa aaaggaactt ccacccatac ttaccaatgg
     9481 tagtgtaact cctgtaacag gtaacaggtt tacattaaca gccatattgg ctattgcttg
     9541 tattaccaat gtaaagctta aagcaagtgc taaaaatgca ccaaatgcat aagggcatct
     9601 tctaaaaatt ttgatacatc taaacaaaaa agacaaataa ataaatatga taacggcagc
     9661 accaagtaaa ccatattctt caataattat tgaataaata aagtcgttat aagcttgtgg
     9721 taaaaaattt ctttgctcac tattacctgg gccctgacca attaaaatgc ccccctttgc
     9781 aatagctatt ttagcttgtt gtacttggta tggaacttct gtatca
//
LOCUS       DAOQPK010000069         9749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_3712_length_9749_cov_4.714875, whole genome shotgun sequence.
ACCESSION   DAOQPK010000069 DAOQPK010000000
VERSION     DAOQPK010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9749
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3712_length_9749_cov_4.714875"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..1642
                     /locus_tag="PLU36_04815"
     CDS             155..1642
                     /locus_tag="PLU36_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ46103.1"
                     /translation="MNQGLSKKRVKKTNPKALGISIMQEENKRFQVICSSKNDAIITS
                     DETKLITFWNKGAESIFGYTAEEAVGKPMTLIIPNQYQQRHNDGIERMNQRKKPRAIG
                     KVLELSAVRKNGEEFPIELTLGSWDSDGKRYYSGIIRDISAKKKAEKIMLEDKRKIEE
                     QNKRFQVICSSKNDAIITSDESKLITFWNKGAENIFGYTAEEAVGKPMTLIIPMQYQQ
                     RHNDGIERMNQRKKPRVIGKVLELSAVRKNGEEFPIELTLGSWDSDGKRYYSGIIRDI
                     SAKKKAEKLIIEEKKKSDELLLNILPKLVADELKNTGIATAKNHKNVTILFSDFEGFT
                     TLASSISAAELVKELNEIFGQFDDILESLSIEKIKTIGDAYFAACGLPEKNTEHAERC
                     IEAAKQMFRYLEVRNKKTEIKWKMRVGIHSGPVMAGVVSKKKYAYDLFGDTVNIASRM
                     ESNGEVGKINISESTYQLIKKKYKFTPRGHVKTKGKGELKMYFVD"
     gene            1765..2343
                     /locus_tag="PLU36_04820"
     CDS             1765..2343
                     /locus_tag="PLU36_04820"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HMZ46104.1"
                     /translation="MIKFKEAKEFITQKQLEEIPKHFTYHSLVHILIVYESCKVLAEG
                     EGITGIDLQLLLTAALFHDSGFIIGPKNHEETSCEFARQYLPQFNYTNEQIEHICDMI
                     MATKIPQSPKDHLGQILADADLDYLGRDDFYTNGNKLFEELKTLNVLKTEREWYQLQI
                     NFLEKHNYFTATAIRLRKEKKHIHLNELKNKL"
     gene            2373..3260
                     /locus_tag="PLU36_04825"
     CDS             2373..3260
                     /locus_tag="PLU36_04825"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HMZ46105.1"
                     /translation="MRKHSITNHFKDSIYIALGILSATFALNSLLVPNQFLDGGVTGI
                     SLLLSHYFHAPLSIIIVLANIPFIILGAFTAGKKFAIKTFVSIIVLGLCLQFAPISDI
                     TADWTGNMILVSLFGGFFIGLGVGLGMRGGCSIDGIEILAIYTKRKIGFTMSELILAL
                     NVIIFLIAAFSFGMEKAFYAILTYYVATRTIGYVVEGIEEYTGITIISEHSESIKSLL
                     VNELGKGISVYKGQRGFMKENFDVRYDCDIVFTIVTRFEVRRVRNMVHLLDPKAFVFT
                     HTIKETAGGVLKKVAADKH"
     gene            complement(3268..3615)
                     /locus_tag="PLU36_04830"
     CDS             complement(3268..3615)
                     /locus_tag="PLU36_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HMZ46106.1"
                     /translation="MIVIKRIYEHPSSKDGYRVLVDRLWPRGVSKQAAKIDLWCKEIA
                     PSPELRKWFAHDEKKWIGFKNKYTLELEQNIEQAEKLKTLEKENKHITLLYAAKNPLC
                     NHALVLNDFLSKL"
     gene            complement(3640..4083)
                     /locus_tag="PLU36_04835"
     CDS             complement(3640..4083)
                     /locus_tag="PLU36_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018625881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HMZ46107.1"
                     /translation="MNNKRITINKATPKAFDAMLVLEEFGKTCSIHPILKELIKIRAS
                     ILNGCAYCLDMHTEDAISLGENARRIYAVSVWHESHLFNEEERTVLRITDEVTKIADK
                     GLTDSTYQKAIELFGESATAEIIMLIVTINAWNRIAVSTRLIYKP"
     gene            complement(4085..6859)
                     /gene="acnA"
                     /locus_tag="PLU36_04840"
     CDS             complement(4085..6859)
                     /gene="acnA"
                     /locus_tag="PLU36_04840"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HMZ46108.1"
                     /translation="MNNIQSKEKKEIIINSKRYLYSSLKNYSSIVEHLPFSIRILLEN
                     VLRNYDGFSITNEHVETLLNWTPNPTDKEIPFKPARILMQDFTGVPAVVDMASLRAEF
                     VRHGKDGQKINPAIPVDLVIDHSVQVDYFGTDYSYNKNVELEFERNKERYELLKWAQK
                     GLNNFTVVPPGMGICHQVNLEYLSKVTIEREGWLFPDTLVGTDSHTPMVNGIGVIAWG
                     VGGIEAEAAMLGQPIFFTCPEVVGLKLVGNIPNHCTATDMVLTITKILRDRGVVGKFV
                     EVFGDGLNNLTVTDRATIGNMSPEFGCTVTYFPVDDRTLEYMKATNRSSEQINIVEAY
                     CKENLLWRTGNEKIKYSDIVELNLNTLEPTVSGPKRPQDKIFVKDLSAKFSEILKNEF
                     KREYENKNQRKEAAWLMEGGSGTEFVFGKVPIHGESHSEVVLDSKLHSVRIKEKNKEF
                     VVSDGSIVIAAITSCTNTSNPAVMVGAGLLAKKAVEKGLRTKSWVKTSLAPGSKVVTK
                     YLNRSGLSADLDALRFHTVGYGCTSCIGNSGPLPPAIAMAVDKGELVVASVLSGNRNF
                     EARVHPQVKMNFLMSPMLVVAYALAGRVDIDLINEPIDYDPNGNPVFLKDIWPSITEI
                     QHTINECLKQDDFKEIYDEIFDGSADWQNLEINLNQSFNWNNTSTYIKESPFFENLQA
                     TPNPITEITNARVLLYLGDSVTTDHISPAGSFKEDSAAGIYLKQNGILKDDFNSYGSR
                     RGNHEVMMRGTFANVRIKNKIADKEGGFSKHFPTNQVKTVFETAMQYKEDQTALIVLA
                     GKEYGSGSSRDWAAKGTFLLGIKAVIAESFERIHRSNLVGMGVAPLTFLNNQNAELLG
                     LDGSEVYTITGLADNLTPHKIVDVCAARADGKQINFKVKARLDSAIEIEYYKNQGILQ
                     YVFREYLKNN"
     gene            complement(7041..7451)
                     /gene="rnk"
                     /locus_tag="PLU36_04845"
     CDS             complement(7041..7451)
                     /gene="rnk"
                     /locus_tag="PLU36_04845"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HMZ46109.1"
                     /translation="MGQIILSKLDYTRISESIKHAKQLGKIAATEASKLLTELSNAKV
                     VESQDIPADVVTMNSIIKISFLSSDKVVQFQLVYPDNANIKENKISIFSPIATALIGY
                     KVGDEIEWVVPSGLTKIKIIEIIYQPEAAGDFDL"
     gene            complement(7526..8992)
                     /locus_tag="PLU36_04850"
     CDS             complement(7526..8992)
                     /locus_tag="PLU36_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HMZ46110.1"
                     /translation="MKTELLEFENKHKLLEPTSTERVATLEAISEYTENFLGNIDTLP
                     AFVNNVSEKAPFIQSINFEEQNTLNEVLKNIRLYIDTPGINPASGGHLGYIPGGGLFY
                     GAVGDYLACITNRYAGVRFASPGAVLLEETVLNWMANLFGYSKNYGGTLTSGGSIANL
                     TAIATARDAINLKAKDYHKAVIYTTQYAHHCLQKSIRILGMDDVQWREIECNNLYQMN
                     VEELEKAINKDLNNGFLPWLLVASAGTTDVGAIDPLQQLSEISKKYNLWFHVDAAYGG
                     FFILTNKGKQLLKGIEQADSIVADPHKGLFIPYGLGAVLIKNKKLLHKTHQYLANYMQ
                     DASTEKVDLSPADLSPELSRHFRALRLWLPLKIHGEKPFENYLNEKMQLATYCYQQLS
                     NLPDVEVFNKPELSVFAFRFLHKELPLDKYNEKIQKAVMNDGRVFISSTRLNNCYVLR
                     IAILSFRTHLSTIETALTVLDKSKKIAEDELYQELRNN"
     gene            complement(9091..9522)
                     /locus_tag="PLU36_04855"
     CDS             complement(9091..9522)
                     /locus_tag="PLU36_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HMZ46111.1"
                     /translation="MLSLSCKAAIKAVIFLGSKFETEEKFSIKTIAGFINENEHTVGK
                     LLQKLVKENIINSTKGPNGGFFISAKQINQPVIKIVEAIDGKDIFKQCGLGLEKCSES
                     HPCPFHNNFKPIRDMFKKLCEEKKVIDLYQNVNEGLAYLTN"
     gene            complement(9525..>9749)
                     /locus_tag="PLU36_04860"
     CDS             complement(9525..>9749)
                     /locus_tag="PLU36_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HMZ46112.1"
                     /translation="GLIYELHFDEEENRLTCTMTLTTEFCPMGESITDNARHALQQAY
                     GDYGIDINLIFDPPWNMEMISDEGRAFLGR"
BASE COUNT         3285 a         1557 c         1549 g         3358 t
ORIGIN      
        1 ataaatagca atcagaataa tagtagcaag aaaaacatta aagagcatgt ggtctttatt
       61 ggtaaatttt ctttattttt gatatgctgc aaattaacta tttaaagtct gatgttcttg
      121 tttgtataaa aaattatcca tacaaattaa tcatatgaac caagggcttt caaaaaaacg
      181 ggtaaagaaa acaaatccta aagcgttagg tattagtatt atgcaagaag aaaacaaaag
      241 gtttcaggta atatgtagtt cgaaaaatga tgctatcatt acctctgatg aaaccaagct
      301 aattacattt tggaataagg gggcagaaag tatttttggt tatactgcag aggaagctgt
      361 aggaaagccc atgactttaa taattccaaa tcaatatcag caaaggcata atgatggaat
      421 tgaaagaatg aaccaacgaa aaaagccaag agcaattggt aaggttttag aattaagtgc
      481 cgttagaaaa aatggcgagg aatttccgat agaacttaca ttaggtagtt gggatagtga
      541 tggtaaaagg tattatagcg gaattataag agatatttct gcaaaaaaga aagcagaaaa
      601 aattatgctt gaagacaaaa gaaaaataga ggagcaaaat aaaaggtttc aagtaatttg
      661 tagttcaaaa aacgatgcta tcattacctc tgatgaaagc aagctaatta cattttggaa
      721 taaaggggca gaaaatattt ttggttatac tgcagaggag gctgtaggaa agcccatgac
      781 tttaataatt ccaatgcaat atcaacaaag gcataatgat ggaatagaaa gaatgaacca
      841 gcgaaaaaaa ccacgtgtta taggaaaggt tttagaatta agtgccgtta gaaaaaatgg
      901 cgaagagttt ccgatagaac ttactttagg cagttgggat agtgatggta aaaggtatta
      961 tagcggaatt ataagagaca tttctgcaaa aaagaaagca gagaaattaa ttattgaaga
     1021 aaagaaaaaa tcggatgagt tattattaaa catattaccc aagctggtgg cagatgaatt
     1081 aaaaaatact ggaattgcca ctgctaaaaa ccataaaaat gtaacaattc ttttttcaga
     1141 ttttgagggc tttaccacat tagcatcttc tatttctgca gcagaattgg taaaagaatt
     1201 aaatgaaata tttgggcagt ttgatgatat tttagaatcc ttatcgatag aaaaaattaa
     1261 aacaattggc gatgcttatt ttgctgcatg tggcttgccc gaaaaaaata cagaacatgc
     1321 cgaacgctgt attgaagcag caaaacaaat gtttagatat ttagaagtaa gaaataaaaa
     1381 aactgaaatt aaatggaaaa tgagagtagg cattcattct ggcccagtta tggcaggcgt
     1441 ggtaagtaaa aagaaatatg cttacgatct ttttggcgat acagtaaata ttgccagcag
     1501 aatggaaagc aatggtgaag ttggaaaaat taatatttcc gaatctacct atcagttaat
     1561 aaagaaaaaa tataagttta caccaagagg gcatgtaaaa acaaaaggaa agggggagtt
     1621 aaaaatgtat tttgttgatt aaaatgtttt caaaaatata gtatttataa ggatagggtt
     1681 tattacaaaa aatttgtttg tatttaaaaa taaattattg ataagttgga aagttttcaa
     1741 taattttaaa aataaattta attagtgata aagtttaaag aggcaaaaga gtttattaca
     1801 caaaagcaac ttgaagaaat accaaaacac tttacttacc atagtttagt gcatatttta
     1861 attgtttatg aaagctgtaa agtgttggcc gaaggagaag gaattacagg gattgattta
     1921 caactattgc taaccgctgc actttttcat gattcgggtt ttattattgg cccaaaaaat
     1981 catgaagaaa catcttgtga gtttgcacgt caatatttac cacaatttaa ttacaccaat
     2041 gaacagatag agcatatttg cgacatgatt atggcaacaa aaataccaca atcacctaaa
     2101 gaccacttag ggcaaatact tgcagatgcc gatttagatt atttaggcag agatgatttt
     2161 tataccaatg gtaataagtt atttgaagaa ttaaaaacac tgaatgtttt aaaaaccgaa
     2221 agagagtggt accaactaca aataaatttt ttagaaaaac ataattattt tactgctact
     2281 gcaattaggc ttagaaaaga aaaaaagcat attcatttaa acgaattaaa aaataaacta
     2341 taatcaaaac atcatttata gtaaattaga aaatgaggaa gcatagtatt accaatcatt
     2401 ttaaagacag tatatatatt gctttaggca ttttaagtgc cacgtttgca ttaaatagtt
     2461 tactagtacc caaccagttt ttagatggtg gtgttacagg tattagcctt ttattaagcc
     2521 attactttca tgcaccattg agtattataa tagtattggc taatattcca tttattattc
     2581 tgggggcatt tactgctggt aaaaaatttg ctatcaaaac atttgtaagc attattgttt
     2641 taggcttatg ccttcagttt gcacccatta gtgatattac agcagactgg acaggtaaca
     2701 tgattttagt ttctttattt ggtggttttt ttattggtct tggagttggc ttaggaatgc
     2761 gtggtggctg ctctatagat ggaattgaaa ttttagccat ttataccaag agaaaaatag
     2821 gatttacaat gagtgaatta atacttgcac ttaatgtaat tatattttta atagccgcct
     2881 ttagctttgg tatggaaaaa gctttttatg caattcttac ttattacgtt gcaacaagaa
     2941 ctattggtta tgtggttgaa ggtatagaag aatatactgg cattactatt atttctgaac
     3001 acagcgaaag tataaaatcc cttttagtaa atgaattagg gaaaggcata tctgtatata
     3061 aaggtcaaag agggtttatg aaagaaaact ttgatgttag atacgactgt gatattgttt
     3121 ttaccattgt aacccgtttt gaagtaagaa gagttagaaa tatggtacat ttattagacc
     3181 ccaaagcctt tgtatttaca cacaccataa aagaaacagc aggtggtgtg ctaaaaaaag
     3241 tagctgccga taaacattaa tttttctcta cagtttactt aaaaaatcat tcaatacaag
     3301 tgcatggtta caaagtggat tttttgctgc atataacaat gtaatatgct tgttttcttt
     3361 ttctaatgtt tttaattttt ctgcttgttc tatattttgt tctaattcca atgtgtattt
     3421 atttttaaaa cctatccatt ttttttcatc gtgtgcaaac cattttctta attctgggct
     3481 tggtgctatt tctttacacc ataaatctat ttttgcagct tgtttagata caccccgagg
     3541 ccaaagtcta tcaaccaaaa ctctataacc atctttagaa gaagggtgtt cgtaaattct
     3601 tttaattaca atcatagttt attattttat aaaactcaat taaggtttat aaataagcct
     3661 cgtagaaact gcaattctat tccatgcatt aatggttact ataagcataa ttatttctgc
     3721 tgtggcggat tctccaaaaa gttcaatagc tttttgatag gtagaatccg taagcccctt
     3781 atcagcaatt ttggtaactt catcggtaat tcgtaaaacc gttctctcct cttcattaaa
     3841 caaatgcgat tcgtgccaaa cacttactgc ataaattctt ctagcatttt ctcccaaact
     3901 tattgcatct tctgtatgca tatctaaaca ataagcacag ccatttaaaa tagatgctct
     3961 aatttttatt aactctttta aaataggatg aatggagcat gtttttccaa attcttctaa
     4021 aaccagcata gcatcaaaag cctttggtgt tgctttatta atagttattc ttttgttgtt
     4081 cattttaatt attttttaaa tactctctaa atacatattg taaaatgccc tggtttttat
     4141 aatattcaat ttctatagcc gaatctaaac gagcttttac tttaaagtta atttgtttac
     4201 catctgccct agctgcacaa acatctacaa ttttatgagg ggttaaatta tctgctaagc
     4261 ctgtaatagt ataaacttca gagccatcta accctaacaa ttctgcattt tgattattta
     4321 aaaatgttaa aggagcaacg cccatgccta ctaagttact tctatgaata cgttcaaaac
     4381 tttcggcaat tacagcttta attcctaata aaaacgtacc ctttgccgcc caatctcttg
     4441 atgaaccaga gccatactct ttacctgcta aaacaataag tgctgtttga tcttctttat
     4501 attgcatagc tgtttcaaaa acagttttta cttggttagt tggaaagtgt ttactaaagc
     4561 caccttcttt atctgctatt ttatttttaa ttcgtacatt ggcaaaagtg cctctcatca
     4621 ttacttcatg attacccctt ctggaaccat aggaattaaa gtcgtctttt aaaattccgt
     4681 tttgttttaa atatatgcct gctgcagagt cttctttaaa agagcctgct ggcgaaatat
     4741 gatctgtagt taccgaatcg cctaaatata gcagtactct tgcatttgta atttcggtaa
     4801 ttgggtttgg cgttgcttga agattttcga agaagggaga ctctttaatg taagtggatg
     4861 tattattcca attaaaactt tgatttaagt ttatttctaa attttgccaa tcggcagaac
     4921 catcaaaaat ttcatcataa atttctttaa aatcatcttg ctttaaacat tcattaatgg
     4981 tatgctgaat ttctgtaata cttggccaaa tatcttttaa aaatacaggg ttgccatttg
     5041 ggtcgtaatc tataggttca tttattaaat caatatcaac ccttcctgct aaagcataag
     5101 ctactactag cattggcgac attaagaaat tcatttttac ttgtgggtgc actcttgcct
     5161 caaaatttct attgccagat aaaacagagg ctacaactaa ttcgccttta tctacagcca
     5221 ttgcaatagc tggaggcagg gggcccgagt ttccaataca agaagtacag ccatagccta
     5281 cagtatgaaa acgtaaggca tctaaatctg ccgataggcc agagcggttt aaatattttg
     5341 taaccacttt agagcctgga gctaatgaag tttttaccca ggattttgtt cttaaacctt
     5401 tttcaacagc ttttttagct aataagcctg caccaaccat tactgcaggg ttagaggtat
     5461 tggtgcaact tgttatagct gcaattacaa tactaccatc gcttacaaca aattctttat
     5521 ttttttcttt tattcttaca gagtgaagct tgctatctaa aacaacttca gaatggcttt
     5581 cgccatgaat aggaactttg ccaaaaacaa attcagttcc agagcctccc tccattaacc
     5641 atgcagcttc tttacgttgg tttttatttt catactcacg tttaaattca ttttttaaaa
     5701 tttcactaaa cttagcactt aaatctttta caaaaatttt atcttgtggg cgttttgggc
     5761 cagatactgt aggctctaaa gtgtttaagt ttaattcaac aatatcagaa tattttattt
     5821 tttcattacc ggtacgccat aataaatttt ctttacaata ggcttctact atatttattt
     5881 gttcgctact tctattggta gctttcatgt attctaatgt tctatcatca acaggaaaat
     5941 aagttacagt acacccaaac tctggagaca tgttgccaat agttgcccta tcggtaactg
     6001 ttaggttatt taagccatct ccaaacactt caacaaattt gcccaccaca cctctatctc
     6061 ttaaaatttt tgtaatggtt aaaaccatat cggttgcagt acaatgattt ggaatattgc
     6121 ctactaattt tagcccaacc acttcgggac aagtaaagaa aattggctgc cccagcatgg
     6181 ctgcttctgc ctcaattcca ccaacgcccc atgcaataac accaatacca tttaccattg
     6241 gtgtatgaga atcggtgcca accaatgtat ctggaaataa ccaaccttct ctttcaattg
     6301 taactttaga taagtactct aaatttacct gatggcaaat gcccatacca ggaggcacta
     6361 cagtaaaatt gtttaagccc ttttgtgccc attttaaaag ttcataacgt tctttatttc
     6421 gttcaaattc taattcaaca tttttattgt aagaataatc tgttccaaaa taatcaacct
     6481 gaacggagtg atcaataact aaatcaacgg gtatagcagg atttattttt tggccatctt
     6541 taccatgcct aacaaactct gcacgaagcg aagccatatc taccactgct ggtacacctg
     6601 taaaatcttg cattaaaata cgagcaggct taaaaggaat ttctttatct gtagggttag
     6661 gagtccagtt taatagggtt tctacatgtt cgtttgtaat actaaaccca tcgtaatttc
     6721 ttaatacatt ttctaataat atacgaatac taaaaggcaa atgctccaca atactggaat
     6781 agttttttaa agaactatat aaatagcgtt tgctgttaat aataatttct tttttttctt
     6841 ttgattggat gttattcata atagtttttt tgttgaagct taaacaaata acaattgcat
     6901 aattattttt tgaaaaaaaa taagcaacga ttaattaaaa agagaaagta aatatactta
     6961 attatttata gttaagcttt tgaaatgcat aaaaaaagtg ctgccattaa gcaacacttt
     7021 atctataata aatagctggt ttataaatca aaatctcctg ctgcttctgg ttgataaatg
     7081 atttcaataa tttttatttt agttaaacca gatggcacaa cccactctat ttcatcgcca
     7141 actttgtagc caattaatgc ggtagctatt ggagaaaaaa tagaaatttt attttctttg
     7201 atattggcat tatcgggata aacaagttga aattgaacta ccttatcgga gcttagaaaa
     7261 ctaattttaa taatggagtt catagtaaca acatcggcag gtatatcttg agattcaaca
     7321 acttttgcat tgcttaattc tgtaagtagt ttagaagctt ctgttgcagc aattttacct
     7381 aactgttttg catgttttat actttctgaa attctggtat aatctaattt gcttaaaata
     7441 atttgaccca taatatttgt tttttaatag aatggataaa gatagttaga tatttaaaaa
     7501 agaaaaacta aaatacgcat tttgattaat tattcctgag ttcttggtat agttcatctt
     7561 cggcaatttt tttactttta tctaaaacag ttagagcggt ttctattgta cttaaatgtg
     7621 ttctaaaaga taaaattgca attcttaaaa cataacaatt atttaaacgg gtggaagaaa
     7681 taaaaacacg gccgtcattc attactgctt tttgaatttt ttcgttatac ttatctaagg
     7741 ggagttcttt gtgtaaaaaa cgaaatgcaa aaacacttaa ttctggttta ttaaaaactt
     7801 ctacatctgg caagttgctt aattgctgat aacaataagt tgctaattgc attttttcgt
     7861 ttaaataatt ttcaaatggc ttttctccat gaattttaag tggcaaccat aatcttaagg
     7921 ctctaaaatg cctacttaac tcaggcgata aatctgcagg agaaaggtcc accttttctg
     7981 tagaagcatc ttgcatatag tttgctaaat actgatgtgt tttatgtaaa agttttttat
     8041 tttttattaa cacagctcct aaaccatagg gtataaataa acccttatgg gggtcagcaa
     8101 ctatagagtc tgcctgttca attcctttta aaagttgttt tcctttgttg gtaagaataa
     8161 aaaatcctcc ataggctgca tctacatgaa accaaaggtt atatttttta cttatttcgc
     8221 ttagttgttg tagggggtct attgcaccta catctgtagt acctgcagag gctactaaaa
     8281 gccagggtaa aaagccgttg tttaaatctt tattaatagc cttctccaat tcctctacat
     8341 tcatttggta taaattatta cactcaattt ctctccattg tacatcatcc attcctaaaa
     8401 tacgaataga tttttgtaag caatgatgag catattgtgt tgtataaata acggctttgt
     8461 ggtaatcttt tgcttttaaa ttaattgcat cacgtgcagt tgcaatagca gttaaattag
     8521 caatactacc acccgaggtt agtgtgccac catagttttt ggagtaacca aataagtttg
     8581 ccatccagtt taaaacagtt tcttctaaca atactgcacc aggcgatgca aacctaacac
     8641 ctgcatacct atttgtaatg caagctaagt aatcgccaac agcaccataa aataaaccac
     8701 cacctggtat atagcctaaa tgcccgccag aggcaggatt tatacctggt gtatcaatat
     8761 ataatcgtat attttttaat acttcattta aagtattttg ttcttcaaaa ttaatactct
     8821 gtataaatgg agctttttct gaaacgttgt ttacaaatgc aggcagagta tctatattac
     8881 ctaaaaagtt ttcggtgtat tcagaaattg cttctagtgt agctaccctt tcggttgaag
     8941 ttggctctaa taatttatgt ttattttcaa actctaacag ttcggttttc atttttaata
     9001 aataattatt ctatatgttt aaaacataaa tataggccac cgttctaggg agtataaatt
     9061 tattatataa atggaaaact tagaaaataa ttagttggtt aagtaggcta aaccttcgtt
     9121 tacattttga tagaggtcta ttactttttt ttcttcacat aattttttaa acatatctct
     9181 tattggttta aagttattgt gaaatgggca tggatgcgat tctgagcatt tttctaatcc
     9241 taaaccacat tgtttaaaaa tatcttttcc atcaatagcc tctacaattt taattacagg
     9301 ctgattaatt tgtttagcag aaataaagaa accgccattg ggtccttttg tgctattgat
     9361 aatattttct tttactaatt tctgtaaaag ttttcctacg gtatgttcat tttcatttat
     9421 aaaaccagca atggttttaa tactaaattt ttcttcggtt tcaaattttg aacctaaaaa
     9481 aattactgct ttaatagcag ctttacaact taaacttaac ataactatct tcctaaaaat
     9541 gcccttcctt catcagaaat catttccata ttccaaggag ggtcaaaaat taaattaata
     9601 tcaattccgt aatctccata agcctgttgc agtgcatgcc ttgcattatc tgttatcgat
     9661 tctcccattg ggcaaaactc tgtagttagg gtcattgtac atgttagcct attttcttcc
     9721 tcatcaaaat gtagttcata aattaagcc
//
LOCUS       DAOQPK010000070         9649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_3774_length_9649_cov_3.869710, whole genome shotgun sequence.
ACCESSION   DAOQPK010000070 DAOQPK010000000
VERSION     DAOQPK010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9649
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3774_length_9649_cov_3.869710"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1179
                     /locus_tag="PLU36_04865"
     CDS             172..1179
                     /locus_tag="PLU36_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HMZ46113.1"
                     /translation="MRVISKLFFLTVTVGLLSVSTLKAQVDPHFSQYYMYPSWLNPAL
                     TGAFDGDYRVTGIYRSQWGNITNPFATPGVSVDFNGNNSLNYGGSVMQQTAGNGGYGY
                     LTAYGNIAYTGVKFGATGTQRLVFGLQAGIIQRRFNPNKMQWGSQWDPSMGYNPNLPA
                     DILARTSATAFDAGAGVLYYDAQPGKKINVYGGFSAAHLTRADDKFSSNGTEKFPIRY
                     TVHGGVKIAINENFTLTPNFLYLRQRTSSQAMLGAYGQIKAPFETEFLFGLNYRFKDA
                     VSPYIGLYYKNMTLGLSYDINSSDLSKLQRGSNSFEISLSFIGRKRAKTPEVEFVCPR
                     L"
     gene            1222..3243
                     /locus_tag="PLU36_04870"
     CDS             1222..3243
                     /locus_tag="PLU36_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ46114.1"
                     /translation="MKKLLLIFVSAFIALNSSAQIVYDYIKAADAYYEKADYYSAAEY
                     YEKYVNSANKKVKADAYNPYTVNALSKKQKVAVSNYQQAVYKLAECYRHLNYHVKAEP
                     LYAQASNFDKAVFPLAPYWHGKTLRALAKYSAADTVLTKFVANGSGGNEYMEDAGREL
                     KNLKFIQEQLVKPDLHLYTVKKSASGINTIGANYAPVKVNANTYLFTSTRNDSGAAKN
                     SAHYNRIYTASFTDGVLHEVKKADIPQSNNSHQGVVAITPNGNIVYLTRWIISGGKKA
                     AALYSSKKENEKWSEPALLDNSINQPGANAQQPCVMPDGKNLIYASDRKDGLGGFDLW
                     SVKLNEEGNIVAGTETNLGATVNTANDEQAPYYHAASQTLVFSSNGYVGMGGYDFYAS
                     KGEIGNWSTPSNLGYPLNSVKDDLYFTTNGNAKNILGDVMFSSDRESECCLEMFTLNK
                     VRPIKQIAGIVIDCETKQPIKGASVQVTNSTTKLISTNTTGEDGTYGFSTEDFDTFTS
                     SASATGYHPNTVTTAAIADDLIIEQKLDVLCLQKIPPPDTPPPPVDTVIVMDNIYFAF
                     NKATILEESHAAIDNQIVTLMNKYPTMVIEISGHTDSKGKDDYNMKLSQARANAVKNY
                     LIAKGIAAERMTAVGYGETKPIAPNQIDGKDNPEGRKKNRRTEFKVLHY"
     gene            complement(3683..4876)
                     /locus_tag="PLU36_04875"
     CDS             complement(3683..4876)
                     /locus_tag="PLU36_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HMZ46115.1"
                     /translation="MATISFDNTENAFAYKSDKDLSRAKFLFNTMNYPFFVPLGTRIT
                     PILVKGKFPFINKLIRDTIFKQFVGGESLEATKVTCELLKKYDVQVILDYAVEGKEGE
                     ANFDHATDVFIKVINFAATQSNIPFISVKVTGIARFGLLETLNEAPRLRSGVHDHEEE
                     SAELERVKDRMYAICSAAAEKNIGVLIDAEDSWIQDPIDKLTMEMMAEFNKEKVVVYN
                     TMQLYRHDRLEFLKLSHKIAQQQNFLLGAKLVRGAYMEKERERALSKNYLSPIQATKE
                     DTDRDFNLAVRYCIENINSVSTIIASHNEQSSMLGYELLKGKNLPFNHPHVHFSQLFG
                     MSDNITFNLAKEGCNVSKYLPFGPIKDVIPYLMRRAQENSSVKGQTGRELTLIKREVA
                     RRKLK"
     gene            5236..5604
                     /locus_tag="PLU36_04880"
     CDS             5236..5604
                     /locus_tag="PLU36_04880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46116.1"
                     /translation="MKKIVVDLDDTISYTTNGDYKNSIPDEALISKLKEYKQNGFEIV
                     IHTSRNMRTFNGSIGKINANTLPIIIDWLNKHHVPYDEIITGKPWCGFEGFYIDDKSI
                     RPDEFKKMSYEEISKLIQSK"
     gene            5719..7173
                     /locus_tag="PLU36_04885"
     CDS             5719..7173
                     /locus_tag="PLU36_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46117.1"
                     /translation="MLQKESLHFEEQVYISLPKSFNINIADEKLLIENNIKIIQVPDN
                     LSIGLSIFYSLNKIKERDEPIRILYGDTLITNLPLFSNFYALGKPEYNYEWASINNNE
                     VSNLTYSGYFSFENIYQLIDSLYACDFDFIKSIKHYKSLCNVKEEICNNWFDLGHINS
                     YYRSKSKLTTERAFNAITIEERVLTKTGDNHQKIEAEAFWFEQLPNEIKVFAPVYLGR
                     NSNNNSQISYKLEYLFLSSLNELFIFGTQPEFVWEIIFNKLTLFLEKAASIKPNNNLD
                     LLHNSIDLYLGKTTERLHKLKDQTGIDINSTWIFNEIEVPSLIAIAQETSKSISTATE
                     KNIGLLHGDFCFSNILFDFKSIDIKLIDPRGLTTSNEFSIYGDTRYDIAKLSHSIIGC
                     YDHIIAGYYSLEYHQENKINFQLHTSNIHKTIQQKFLDTQIGQYSVSSSENYAIMIHL
                     FLSMLPLHYENKKKQLALLSNALRLYLIYKKMFN"
     gene            7173..7910
                     /locus_tag="PLU36_04890"
     CDS             7173..7910
                     /locus_tag="PLU36_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ46118.1"
                     /translation="MYIVIPMAGLSNRFFNAGYKQPKYMLPIDGKTMFEFTIGSFKNY
                     FNNANFIFVIRDVFDTFSFVKSKIKQLGISNASIKVLNHETEGQAETVFWAVNEILDK
                     REELLIFNIDTYRPNFQLPEIAKFCNGYVEVFNGEGEHWSFAEAIDDNSTIVKRTTEK
                     QRVSDLCSTGLYYFKEIALFEETYLYNKKNNIRVNNEFYVAPLYNFLIENGYKIHYHL
                     INNNEVEFLGTPEEYTILINKFKHNNL"
     gene            7921..9072
                     /locus_tag="PLU36_04895"
     CDS             7921..9072
                     /locus_tag="PLU36_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46119.1"
                     /translation="MKIALILTGNLRSYKTAYNSLVENIIDPLQPDIFIHTWETQDAN
                     TPTWWNNNLIAEPEKTSYEDLQQMYNPQKILIEANKDFQLKHSVPSRINMPGIYSMFY
                     GWQKGFEIMKQFEEINNFEYDIVIRTRFDILLSQKITIDLLTKCINENMICLPYSDTW
                     FMLGGFVDTFIITNRKIAEKCICLLYNFDIFTENFNSFRHNFFIPEIYFGECLNNNGI
                     NTFPLQQQISLLRKEGKNPTFNEQLLRLGNKLQYAIFFISGLGPDLSKDKEAYKKEIE
                     DVILEYSYLKPGEANLLATYLSNPNKTSNSILWKITWNVNKWNIDKVGGFDRNVFIRT
                     LFQKIILKNPSKKRNQLLLVIIASIKTNFIFYNSKMHFLSTLKKYFKGK"
     gene            9140..>9649
                     /locus_tag="PLU36_04900"
     CDS             9140..>9649
                     /locus_tag="PLU36_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HMZ46120.1"
                     /translation="MNVGSKKANETIAIMNELVLPNDTNVFGNLMGGRLMYWMDIAAG
                     ITAGKHCNAPSVTASVDNLSFKNPIKLGNVVHIESKVTRAFNTSMEIHIKVWGEDLIA
                     QYKYESNEAYFTFVALDPNGNPRAVPSLIPESDEEKKLYDGALRRRQLRLILAGKMKP
                     NDANELKNLF"
BASE COUNT         3540 a         1350 c         1508 g         3251 t
ORIGIN      
        1 tataatagta atagatattc tgctagttgg gatggaagag tgaatggaaa acctattcct
       61 gttggtgtgt actattatat tattgacttt agaggacatt atccaacatt aaccggatca
      121 ttaacaatac ttagataaaa caaattaacc taacaataaa agcaaacgat aatgagagtt
      181 atttctaaat tattttttct tactgtaact gtgggcttgc tttcagtttc tacactaaaa
      241 gcacaggtag atccacattt ctctcaatat tatatgtacc caagttggct aaatccggct
      301 ttaacaggtg catttgatgg agattacaga gttactggta tttatagaag ccaatggggt
      361 aatattacta acccttttgc tacaccaggt gtttctgttg attttaatgg caacaattcg
      421 ctaaattatg gaggaagtgt aatgcaacaa actgctggaa atgggggcta tgggtattta
      481 acagcttatg gtaatattgc ttatactggt gttaaatttg gtgctacagg tacacaaaga
      541 ttggtttttg ggttacaagc aggaattata caaagaagat ttaatcctaa taaaatgcag
      601 tggggtagcc aatgggatcc tagtatgggc tataacccaa acttacctgc agatatttta
      661 gctagaactt cggctactgc ttttgatgct ggtgctggtg ttttatatta cgatgcacaa
      721 ccaggtaaaa aaataaatgt ttatggtgga ttttctgctg cacacttaac cagagcagat
      781 gacaaattta gctcaaatgg tacagagaaa ttccctattc gttataccgt acatggaggt
      841 gttaaaatag ctatcaacga aaattttaca ttaactccta acttcttata cctacgtcaa
      901 agaacttctt ctcaagctat gcttggagct tatggtcaaa taaaagcacc atttgaaaca
      961 gagtttttgt ttggattaaa ttatagattt aaagatgccg tttctccata tattggttta
     1021 tattataaaa acatgacttt gggtttaagt tacgacatca attcatctga tttaagtaaa
     1081 cttcagagag gctctaatag ttttgaaatt tctttatctt ttataggaag aaagagagca
     1141 aaaactccag aagttgaatt tgtttgtcca agattataat tttcgtttat actatatttt
     1201 taaacaggct aatatcatta catgaaaaag ttattgttaa tatttgtttc tgcttttatt
     1261 gctttaaaca gttctgcaca aattgtgtac gattatatta aagcagcaga tgcttattat
     1321 gaaaaagcag attattattc tgcagcagag tattatgaaa aatacgtaaa tagtgctaat
     1381 aaaaaagtta aggcagatgc ctacaatcct tatacagtaa atgcactttc taaaaaacaa
     1441 aaagttgcag ttagtaatta tcagcaagct gtctataaac tggctgagtg ttataggcat
     1501 ttaaactatc atgtaaaggc agagccttta tatgcacaag caagtaattt tgataaagca
     1561 gtatttcctt tagcaccata ttggcatggt aaaacattaa gagctttagc aaaatattct
     1621 gcagcagata ctgtactaac caaatttgtt gcaaatggta gtggaggtaa cgaatatatg
     1681 gaagatgcag gtagagaatt gaagaatttg aaattcattc aagagcaact tgtaaaacct
     1741 gacttgcatt tatatactgt taaaaaatct gcatctggta ttaatactat tggtgcaaat
     1801 tatgcacctg taaaagtaaa tgcaaacact tatttattta cctctacaag aaacgatagt
     1861 ggagctgcca aaaacagtgc acactataat agaatttaca cagcttcttt tacagatggt
     1921 gttttacacg aagtgaaaaa agcagatatt cctcagtcaa ataattctca ccaaggtgta
     1981 gtagcaatta caccaaatgg taatatagtt tatttaacac gttggataat ttctggtggt
     2041 aaaaaagctg ctgcattgta ttctagcaaa aaagaaaatg aaaaatggag tgaacctgct
     2101 ttattagata attccattaa ccaaccaggt gccaatgccc aacaaccttg tgtaatgcct
     2161 gatggtaaaa atttaattta tgcaagtgat aggaaagatg gtttaggagg atttgattta
     2221 tggtcggtta aattaaatga agaaggaaat attgttgctg gtaccgaaac caatttaggt
     2281 gcaacagtta atacagcaaa tgatgagcaa gctccttatt atcatgcagc ttctcaaaca
     2341 ttggtttttt ctagtaatgg ttacgttgga atgggagggt atgattttta tgcaagcaaa
     2401 ggtgaaattg gtaattggtc aactccctct aatttaggat acccacttaa ctctgttaaa
     2461 gacgatttat attttaccac aaatggaaat gctaaaaata ttttgggaga tgtaatgttt
     2521 agttctgata gagaaagtga atgctgttta gaaatgttta ctttaaacaa agttcgccca
     2581 attaaacaaa ttgctggaat agtaattgat tgtgaaacaa aacaaccaat taaaggagca
     2641 agtgtacaag taacaaactc aactacaaaa ctaatatcta ccaatacaac aggagaagat
     2701 ggtacatacg gattttctac ggaagatttt gatacattta cttcatcagc atctgctact
     2761 ggttatcatc ctaatacagt tacaactgct gctattgcag atgatttaat tattgaacag
     2821 aaattggatg ttttatgttt acaaaaaatt ccaccaccag atactccacc accaccagtt
     2881 gatactgtga ttgtaatgga taatatttat tttgctttca ataaagcaac aatattagaa
     2941 gaatcgcatg cagcaataga taaccaaatt gttacactca tgaacaaata tccaacaatg
     3001 gttattgaaa tttctggtca tacagatagc aaaggcaaag acgattataa catgaaatta
     3061 tctcaggcta gggctaatgc agttaaaaat tatttaattg caaaaggaat agctgcagaa
     3121 cgtatgactg ctgttggtta tggcgaaaca aaaccaattg ctccaaacca aattgatgga
     3181 aaagacaatc cggaaggtcg taagaaaaac agaagaactg aatttaaagt gttacactac
     3241 taaaaagtgt ttaaaatgtt aatagtaaat ggctgtctaa aaaaagacag ccatttttgt
     3301 aataaattgg gtttgctttt taaaatgtat ttacacctta tttagtgatg aatgttactt
     3361 taagagtttg cattatatag gattgtatta cacttaatac tgttaaatgt tgccctacat
     3421 ttctatcagg tgtgtaacgt ttgctttaag gctctttata ttgaagaata ggctttaggg
     3481 tgggctataa tgtttatctt tctttacaat gttagatttc tttatgttgg tttcaatatc
     3541 ttaatttaaa taatgataac catcaatatt ctacaaggct tacattgaat attctaaaaa
     3601 actataaaat aaactctaga ttataatatt aacttttaag agtttatcaa taagaacaga
     3661 gtaaaaggat agattttgta cactacttta attttcttct tgcaacttct cttttaatta
     3721 aagtaagctc tcttcctgtt tgtcctttaa cagaagagtt ttcttgtgct cttctcatta
     3781 aataaggtat aacatcttta attggcccaa aaggtaggta tttgcttacg ttacaacctt
     3841 cttttgcaag gttaaatgta atattatcac tcatgccaaa aagttgtgag aaatgtacat
     3901 gaggatggtt gaacggaaga ttttttcctt tcaataattc gtaacccagc atactgcttt
     3961 gttcgttatg gcttgcaata atggtactaa cactatttat attttctatg caatatctaa
     4021 cagctaaatt aaaatctcta tcagtatctt ctttggttgc ctgtattgga gatagataat
     4081 ttttacttaa agctctttca cgttcttttt ccatatatgc accccttaca agctttgcac
     4141 ctaataaaaa gttttgttgt tgtgctattt tatgcgatag ttttaaaaac tctaatctat
     4201 cgtggcgata taattgcatg gtattataaa ccactacttt ttctttatta aactcagcca
     4261 tcatttccat ggtaagttta tcaatagggt cttgaatcca gctatcttca gcatcaatta
     4321 aaacgccaat atttttctca gcagcagcac tacaaattgc gtacattcta tctttaactc
     4381 tttccaattc cgcactttct tcttcatgat catgcacacc acttcttaat cttggggctt
     4441 cgtttaaagt ttctaacaaa ccaaaacggg caatgcctgt tacttttaca cttataaaag
     4501 gaatattaga ttgagttgca gcaaaattta taactttaat aaaaacatcg gtagcgtggt
     4561 caaaatttgc ttcaccttct tttccttcaa cagcataatc taaaataacc tgtacatcat
     4621 atttttttaa taattcacaa gtaacttttg tagcttctaa tgattcgcca ccaacaaatt
     4681 gtttaaagat ggtatctctt attaatttat ttataaacgg aaattttcct tttaccaata
     4741 ttggtgttat tcttgtacca agcggaacaa aaaaaggata attcatggta ttgaataaaa
     4801 attttgctct acttaaatct ttgtcagatt tatatgcaaa tgcattttcg gtattgtcaa
     4861 aagaaatagt agccatataa ataagagtta ttgctgcgaa tatcggtagc tataagatga
     4921 taaaaaaatg tatattctga tgtgtgaata attttttgta tttaaaaaat ggttttgaat
     4981 taatttagga aattaaagtt aattatatga aactagtttt atttttctat ctcttttaac
     5041 cattcaacca aaaatgctat tctcaaattt tttaccaagg taatggaatt aggaatatta
     5101 tcagtctgac gattgtcgtc tgactgataa tataaactat ttaatttcaa ctcttgtgag
     5161 taactaaaaa tcaatgaatt ttagatttgt tatgtattag tattaaaatt aattttactg
     5221 aaaaaagtat aaaaaatgaa aaaaatagtt gttgaccttg atgatacaat tagttacact
     5281 acaaatgggg attataaaaa ctcgatacca gacgaagctc ttattagtaa gctaaaagaa
     5341 tataaacaaa atggctttga aattgtaata catacaagta gaaacatgag aacctttaat
     5401 ggtagtattg gaaaaattaa tgcaaataca ttgcctataa ttatagattg gttaaataag
     5461 catcatgtac cttatgatga aattattacc gggaaacctt ggtgtggttt tgaagggttt
     5521 tatatagatg acaaatctat tagaccagac gaatttaaaa aaatgagtta cgaagaaatt
     5581 tcgaaactaa tacaaagcaa ataaataata ataaacatgg tttaaattag ctcaggtgct
     5641 tatgtagagc cttctttaaa gtctgaattt ggtttaattc caccttcatt tttaccagtt
     5701 agaaataaaa gacttttcgt gttgcaaaaa gaaagtttac attttgaaga acaggtatat
     5761 atatctctcc ctaaatcgtt taatattaat attgcagatg agaagttatt aatagaaaac
     5821 aatattaaga taattcaagt accagataat ttgtcaatag gtttatctat cttttatagt
     5881 ctaaataaaa ttaaagaaag agatgaacca ataagaattt tatatggcga tactttaatt
     5941 actaatttac cattatttag taatttttat gcattaggaa aaccagaata taattatgaa
     6001 tgggctagca ttaacaataa tgaggtatcc aatcttactt actctggtta tttttctttt
     6061 gaaaatatat atcaattaat tgattcactt tatgcttgcg attttgactt tatcaaatca
     6121 ataaaacatt ataaaagcct atgtaatgtt aaggaagaaa tttgtaataa ttggtttgat
     6181 ttagggcata ttaatagtta ctatcgctca aaatctaaac ttacaacaga aagagctttt
     6241 aacgcaataa ctatagaaga acgagtatta acaaagactg gagataacca ccagaaaatt
     6301 gaagcagaag cattttggtt tgaacaatta ccaaacgaaa tcaaagtatt tgctccagtt
     6361 tatttaggaa gaaattcaaa taataattca caaatttcgt ataaactcga atatcttttt
     6421 ttaagttcat tgaacgaact ttttattttt ggtactcaac cagaatttgt ttgggaaatt
     6481 atttttaaca aactaactct tttcttggaa aaagcagcta gtattaagcc aaataataat
     6541 ttagatttac tacataattc tattgaccta taccttggga aaactactga aagactccat
     6601 aaacttaaag atcaaacagg tatagatatt aatagcactt ggatatttaa tgaaatagaa
     6661 gttccctctt taattgctat tgcccaagaa acatctaaaa gcatttctac tgctactgaa
     6721 aaaaatattg gacttttgca tggcgacttt tgcttcagta atatcctatt tgattttaaa
     6781 agcattgata taaaactaat agatccaaga gggctaacaa cttctaatga atttagtatt
     6841 tatggagata caagatacga tattgctaaa ctttctcatt caattattgg ttgttatgat
     6901 catattattg caggttatta ttctttagaa tatcatcaag aaaataaaat caactttcaa
     6961 cttcatacca gcaacattca taaaactata cagcaaaagt ttttagatac acaaataggg
     7021 caatactctg tttcatcgtc tgaaaattat gcaataatga tacatttgtt tttatcaatg
     7081 ttgccattac attatgaaaa taaaaagaaa caattagcac tattatcaaa cgctttaagg
     7141 ttatatctaa tttacaaaaa aatgtttaat taatgtatat agtaattcca atggctggtt
     7201 taagtaaccg tttttttaat gcaggttata agcaaccaaa atatatgcta ccaattgacg
     7261 gtaaaaccat gtttgaattt acaatcggaa gttttaaaaa ttatttcaac aatgcaaatt
     7321 ttatttttgt tatcagagat gtttttgata cattttcttt tgttaaatct aaaataaagc
     7381 aacttggtat ttctaatgca agtataaaag ttttaaacca cgaaacagaa ggacaggcag
     7441 aaactgtttt ttgggcagta aatgaaatat tagataagag agaagaatta ctaattttta
     7501 atattgatac ttacagacca aattttcaat taccggaaat agctaaattt tgtaatggtt
     7561 atgtagaagt ttttaatgga gagggagaac attggtcatt tgcagaagca atagatgata
     7621 attctactat tgtaaaaaga acaacagaaa aacagagagt ttctgactta tgtagtacag
     7681 ggctttatta ttttaaagag attgcacttt ttgaagaaac atatttgtat aataaaaaaa
     7741 ataacataag agtaaataac gaattttatg ttgctccttt gtataatttc ctaatagaaa
     7801 atgggtataa gattcattat catctaatta ataataatga agtagaattt ttaggaactc
     7861 cagaagaata tactatattg ataaataaat ttaaacataa taacttataa taagagatta
     7921 atgaaaattg cattgatact tactggaaat cttagaagct ataaaactgc atataatagt
     7981 ttagttgaaa atattattga ccctttacaa ccagacattt ttattcatac atgggaaaca
     8041 caagatgcca atactccaac ttggtggaat aataatttaa ttgcagaacc agaaaaaaca
     8101 tcttacgaag atttacaaca aatgtataat cctcaaaaaa tactcataga ggctaataag
     8161 gattttcaac taaaacatag tgttccaagt agaattaata tgccgggtat ttacagtatg
     8221 ttttatggat ggcaaaaggg ttttgaaata atgaaacaat ttgaagaaat caataatttt
     8281 gaatacgata tagttataag aacaaggttt gatattttac tttctcaaaa aattacaatt
     8341 gatttattaa caaaatgtat caacgaaaat atgatatgct taccatactc cgatacatgg
     8401 tttatgttag gcggttttgt tgatactttt attattacaa atagaaaaat agctgaaaag
     8461 tgtatttgtt tactttacaa ttttgacatt ttcacagaaa acttcaactc ttttaggcac
     8521 aattttttta ttccagaaat atactttggt gaatgcctga ataataatgg aattaatact
     8581 tttcctctac aacaacaaat aagcctatta aggaaagaag gcaaaaatcc aacttttaat
     8641 gagcaactgc taagattagg taacaagttg cagtatgcta tattttttat ttcaggctta
     8701 ggaccagatt tgtcaaaaga taaggaagca tataaaaaag aaattgaaga tgttatacta
     8761 gaatactctt atcttaaacc aggcgaagcc aatttattag caacgtatct ttcaaaccca
     8821 aataaaactt ccaattctat actttggaaa attacatgga atgtaaacaa atggaatatt
     8881 gataaagtag gcggttttga tagaaatgta tttattcgta cactctttca gaaaataatt
     8941 ctaaaaaatc catcaaaaaa aagaaatcaa ttattattgg taattattgc ttctataaaa
     9001 acaaatttca tattttataa ctcaaaaatg cattttctat caacattaaa aaagtatttt
     9061 aaagggaagt aaaataattt gaattttcaa gtaattcaga tatacgtctt tctcttacct
     9121 tcgccactaa ttgtataata tgaatgtagg ttcaaaaaaa gcaaacgaaa ctattgcaat
     9181 aatgaatgaa ttggttttac caaatgatac caatgtgttt ggaaatttaa tgggaggtag
     9241 attaatgtat tggatggata ttgcagcagg aattacagca ggcaagcatt gcaacgcacc
     9301 aagtgttaca gcaagtgtag ataatttaag ttttaaaaat cctattaaat taggcaatgt
     9361 agtacatata gaatctaagg ttacaagggc ttttaatact tctatggaaa ttcatattaa
     9421 agtttggggc gaagatttaa ttgctcaata taaatacgaa agcaatgaag cctattttac
     9481 ttttgtagct ttagacccca atggtaatcc aagagctgtg ccaagcttaa ttcctgaatc
     9541 agacgaagaa aaaaaattat atgatggagc attaagaaga agacaattaa ggctcattct
     9601 tgcaggaaaa atgaaaccaa atgatgctaa tgaattaaaa aatttattt
//
LOCUS       DAOQPK010000071         9597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_3800_length_9597_cov_4.649864, whole genome shotgun sequence.
ACCESSION   DAOQPK010000071 DAOQPK010000000
VERSION     DAOQPK010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9597
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3800_length_9597_cov_4.649864"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PLU36_04905"
     CDS             complement(<1..426)
                     /locus_tag="PLU36_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HMZ46121.1"
                     /translation="MPQYPNRQFLDFEQPIKELYEQIDQTKKLAEKNPKIDYSNTLHQ
                     LEQSIIDKRKEITLHLTPWQRVQLSRHPDRPYTLKYIEKMTTDFIELHGDRNVKDDKA
                     MVGGFANLNGETVMFIGQQKGINTKSRQLRNFGMANPEGY"
     gene            595..1161
                     /gene="efp"
                     /locus_tag="PLU36_04910"
     CDS             595..1161
                     /gene="efp"
                     /locus_tag="PLU36_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HMZ46122.1"
                     /translation="MATTSDISRGMVLKLDGNLYSVVEFGENKTARAAAKVWAKLKGV
                     DTSRTIEKTWNSGETIHPVRVEKKSFQYLYKDESGFNLMNSETFEQIILAEEMIDAPQ
                     FLKDNGEVFVLINTETDQPIGAELPEKIVVLVTYCEPGLRGDTATRATKPATIDTGAT
                     VNVPLFVEEGELIRINTKTGDYVERVKE"
     gene            1240..2505
                     /locus_tag="PLU36_04915"
     CDS             1240..2505
                     /locus_tag="PLU36_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMZ46123.1"
                     /translation="MKPVAIFLSLFISTIAFTQNSNKKVLFLGNSYTYVNDLPFMLSK
                     IANSTGDTVVYDSNTPGGYTLQGHYTNSTSLSKIGLGKWDYVVLQEQSQLPSFPEIDV
                     ITSVYPYAKKLDSIINEKNNCAETIFYMTWGRKNGDASNCAFWPPVCTYNGMDSLLNL
                     RYQKMADSFNAIVSPVGAVWNYIIKNYTDIELYSSDQSHPSLAGTFAAACSFYAVIFK
                     KDPTKITFNSGLNDIDANRIKTAAKTVVYDSLQKWHVGEYDAKASFTFIDSTNGKIIF
                     KNQSANANSYVWYFGDGNSSSNTNPVHTYTTNGNYTVKLLANKCSVKDSATQTIHIVV
                     SSNPPTANDAKPPSIFPIPATNSIFIQLYNNNLIGKEYNLFSSAGKLVLKGSFKTTKE
                     KIILNKLQPGLYILKIGEKNDNLYKIIKQ"
     gene            2558..3073
                     /locus_tag="PLU36_04920"
     CDS             2558..3073
                     /locus_tag="PLU36_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HMZ46124.1"
                     /translation="MLIKVKTIFACICILITANSFAQLNYKGFLITGNIKQDVFIGLD
                     AIAQTPSVQLKDIVITNHLNVPQLTAKNLKGFLLKEILSKVILNEPLPRKYSEFYFTF
                     IAQDSFTAVFSWNEIFNSPTGNNLFIITQKDGKSVTEMPESVLILTATDFTTGRRYIK
                     KLSAIIVSRAK"
     gene            3187..4131
                     /locus_tag="PLU36_04925"
     CDS             3187..4131
                     /locus_tag="PLU36_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46125.1"
                     /translation="MDKTPTINNNIVKPEENFKAMSNIEKANKKGIKNGFFSFLLVLL
                     LMPIGHAIMILNEKVLHNQKYVGAFIMGFIGIGLIVWGIKKNKKANFATIMGFLGGVL
                     IWTGWVEFSFMWIAEKNNVANLMVNGQIATKREYLVMLSSLGLLGTIMTYFFFTRSNC
                     TFFIWFQNKLGYKNDIAPEGRYFKKPLAITTFIETIMLLWFFYIVLLVVYDDSIAGDK
                     HIATYIVAWGSLIWSIYLISKLITIQVFDYAVRYAVPTVIIFWNFIEVLGRWDTFKEV
                     WIYPIEHWFEVSLMIVLVIGLLFLFLKTPYFKKELRRV"
     gene            complement(4234..4854)
                     /locus_tag="PLU36_04930"
     CDS             complement(4234..4854)
                     /locus_tag="PLU36_04930"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HMZ46126.1"
                     /translation="MFLFVITMQAQHLKPLESENDNYRYKEEKENVAIQPKYEHVGNF
                     YEGFANVLLNKKWGFIDKTGKVVIPIKYDKVDIFSEGLAPVSLNKKWGFIDKTDKVVI
                     SLKYDDANIFSEGLAAVEINEKHGFINQKGKEVISIKYDWADDFSEGFAVVELNGKFG
                     FIDKKGKVVIPFLYESAFPFKDGKAKVEKDGIEFYIDNKGNEIIEE"
     gene            5164..5889
                     /locus_tag="PLU36_04935"
     CDS             5164..5889
                     /locus_tag="PLU36_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HMZ46127.1"
                     /translation="MTELEKNIIAIKRRIEVACINANRNTNSVKLLLATKTVNANKII
                     EAINLGETLIGENKIQEVKEKFEALKNIPYQKHFIGYLQSNKIKDILKYEVNCIQSLD
                     RLSLAEKLHKRLTLEEKKLDVLIQVNTSFEASKFGIAPNNTIDFIKQVANFETLQIKG
                     LMTIGLFSSDADKVRQCFKNLKALQQQIIALNLPNVSMQELSMGMSGDLEIAIEEGAT
                     IIRVGTAIFGKRIYPDSYYWNEG"
     gene            6104..7471
                     /locus_tag="PLU36_04940"
     CDS             6104..7471
                     /locus_tag="PLU36_04940"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HMZ46128.1"
                     /translation="MNISSSAKIDLKSSVLIIFSLIFSFSAISQTTDSSVNNLKVQIQ
                     FRPKLEVRDGAFRPLAKKEEPAVLVSERIRLNINYSYKNIFTLKISPQTVGIWGQANA
                     VQGVENSGNKIALFEAWTNFNLSNTWNIKLGRQVISLDDERFFGELDWAQGGRTHDAL
                     SFLYKKNNVNVKSYFAFNQNYKSLYNNNSYNPAGNLYNSNDAVPYKTMQTLWVGFNAN
                     AISTFSFLFTNIGLQQAANLNNTNAAVHQSQTFGINYFNAGKTTFGNLSAYWQTGKNI
                     AGTKTQAFLLAGSIGYKFSNKTKIMVGTDWVSGNDIGQAKPPTTNNAFTPYFHTGHKF
                     YGNMDYYFAGNGHQNAGLSDSYLNLQCTPTKKLNATIAVHQFYTTNKISSQLKNYSKN
                     LGQELDFSLQYKPNSFTSLSCGYSFYVTTPTIHYLKNTTGSKDYQQWLWLSLNVTPTL
                     LISKF"
     gene            7489..8538
                     /gene="nirK"
                     /locus_tag="PLU36_04945"
     CDS             7489..8538
                     /gene="nirK"
                     /locus_tag="PLU36_04945"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HMZ46129.1"
                     /translation="MKLKRLILIATAPLIVTTYSCKTNPNQTGSTKPAIDMVAEGNPE
                     VAEVTGAPKVPAPVGNRGPKKLIVNLVTTEMEGIIADSIPYTFWTFNHTVPGSFIRIR
                     VGDEVTLNLKNEASSVLPHNIDLHAVTGPGGGAAATSSAPGKTTSFTFKALNPGLYVY
                     HCAAPPVPMHIGNGMYGLILVEPEGGLPKVDKEFYIMQGDFYTKASAIKKGLLEFDND
                     KAVAENPDYVLFNGKKGSLLGANMIEAKVGETVRLYVGNGGPNLTSSFHVIGEIFDNV
                     YLEGGSTISHNIQTTMIPAGGAAIVEFKCDYPGEYVLVDHSLSRAFNKGAIGKLKVTG
                     KADAKVFNAKTIGSH"
     gene            8562..9410
                     /locus_tag="PLU36_04950"
     CDS             8562..9410
                     /locus_tag="PLU36_04950"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HMZ46130.1"
                     /translation="MKINIFLIITVFTLLLYACNNQQKIDSKEIKIANSTTPNNSIPL
                     TVKMVYIPGGEYKPFYGSDSTLVKVPAFLLDERAVTNQEFLAFIKLNPAWRKSVIKRI
                     YADTSYLNNWVNDTTLPKDAKLDAPITNISWFAAKAFAKSIGKRLPTLDEWEYVAMAD
                     KTTANARSKPTYSNEILDLYLQKNRQYNAVKQSAPNYWGVYNMFDLIWEWTDDFNSVL
                     TTGDSRSGQYDDKNLFCAASATSATDIMNYAAFMRFALRGSVKANFAIANLGFRCAKD
                     TLILNK"
     gene            9416..>9597
                     /locus_tag="PLU36_04955"
     CDS             9416..>9597
                     /locus_tag="PLU36_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46131.1"
                     /translation="MKYFFYCLISVVLFSACKENKKQTVQLPSDSIFYLTSQWKNQNG
                     DSLQLKALQGKTLAVV"
BASE COUNT         3413 a         1428 c         1474 g         3282 t
ORIGIN      
        1 atatccttcg ggatttgcca taccaaagtt tcttaactgt ctagatttgg tattaatacc
       61 cttttgctga ccaataaaca ttactgtttc gccatttaag tttgcaaatc cacctaccat
      121 tgctttatcg tcttttacat tcctatcgcc atgtaattca ataaaatcgg tagtcatttt
      181 ttcaatatac tttaatgtat aaggtctatc tggatgacga cttaactgaa ctctttgcca
      241 gggtgttaaa tgcagggtta tttcttttct tttatcaata attgattgct ctaattgatg
      301 tagtgtattg ctataatcaa tttttgggtt tttctctgca agctttttgg tttggtcaat
      361 ttgttcatac aattctttaa taggctgctc aaaatccaga aattgtctgt tggggtattg
      421 tggcatactt tattaattgg tttgtaaaaa taatactttg cgtgtttata ggttataaaa
      481 tcaatatttt tatttcaatt ctatatttct aatggcaaga gtaataaaat taaaacttga
      541 gtttaatttt tttcttgttc aacccttact tttgccactc taaataaata atatatggca
      601 acaacatcag atattagtcg tggaatggtt ttaaaattag atggtaattt gtatagtgtg
      661 gtagagtttg gcgaaaataa aacagcccgt gctgcagcaa aagtttgggc aaaacttaaa
      721 ggagtagata ccagccgtac aattgagaaa acatggaata gtggagaaac tattcaccct
      781 gtaagggttg aaaaaaaatc ttttcagtat ttgtataaag atgaaagtgg ttttaacctt
      841 atgaatagcg aaacctttga gcaaattatt ttagcagaag aaatgattga tgccccacaa
      901 tttttaaaag ataatggcga agtttttgtt ttaattaata ccgaaacaga tcaaccaata
      961 ggtgcagaac taccagaaaa aattgttgta ttagtaacct actgcgagcc aggcttaaga
     1021 ggtgatactg caacaagggc tacaaaacct gcaactattg atacaggtgc aactgtaaac
     1081 gttcctttat ttgtagaaga aggtgaatta attagaataa atactaaaac aggagattat
     1141 gtagaaagag taaaagaata attattcttt tactttaata attataagat tggctactta
     1201 attggtagcc attcgtattt ttaaacgttt ttttaattta tgaaaccggt agcaatattt
     1261 ttaagccttt tcatttctac aatagctttt acacaaaata gcaataaaaa agttttattc
     1321 ttaggtaata gttatactta cgtaaacgat ttgccattca tgctttcaaa aattgcaaac
     1381 tctacaggag atactgtggt ttacgatagt aatacacctg gtggttatac attacaaggg
     1441 cattatacca attctacttc tttatcaaaa attggtttag gcaagtggga ttatgtagtg
     1501 ttgcaagagc aaagtcaatt gccttccttt ccagaaatag atgtaataac tagtgtgtac
     1561 ccctatgcaa aaaaattaga cagtattatt aatgaaaaaa ataattgtgc cgaaacaatt
     1621 ttttacatga catggggaag aaaaaatgga gatgcctcta actgtgcatt ttggccacct
     1681 gtttgcactt acaatggtat ggatagttta ctaaatttac gataccaaaa aatggcagat
     1741 agttttaatg ccattgtttc gcctgttggt gctgtttgga attatattat aaaaaattat
     1801 acagatattg aattgtattc atcagaccaa agtcatccat cacttgcagg tacatttgct
     1861 gctgcatgta gtttttatgc agttattttt aagaaagacc caactaaaat tacatttaat
     1921 agtggcttaa acgatataga tgcaaataga ataaaaactg ctgccaaaac tgtggtttat
     1981 gatagcctac aaaaatggca tgttggcgaa tacgatgcaa aagcaagttt tacttttata
     2041 gatagtacta atggtaaaat aatttttaaa aatcaatctg ccaatgcaaa tagttatgta
     2101 tggtattttg gcgatggtaa tagctcttct aatacaaacc ctgttcatac ttatactacc
     2161 aatggtaatt acactgtaaa acttttagct aataaatgct ccgttaaaga ctctgcaaca
     2221 caaaccattc atatagttgt ttcatcaaac ccacctactg ctaatgatgc taaaccacca
     2281 tctatttttc ctatacctgc taccaactca atttttattc aactatacaa caacaattta
     2341 attggtaaag aatataattt attttcatcg gcaggtaaat tagttttaaa gggaagcttc
     2401 aaaaccacca aagaaaaaat tatattaaac aaattacaac caggtttata tattttaaaa
     2461 attggagaaa agaatgacaa cctatacaag ataattaaac aataaatttt taatctcaaa
     2521 aggcatttta ttttatcttt agtactacaa attaattatg ttaataaagg ttaaaacaat
     2581 ttttgcttgt atatgtattt taattactgc taacagtttt gctcaattaa attataaagg
     2641 atttttaatt acaggaaata taaaacaaga tgtttttatt gggcttgatg caatagccca
     2701 aaccccttct gtacaactta aagatatagt aattactaac cacttaaatg taccacaatt
     2761 aactgcaaaa aatttaaaag gatttttatt aaaagaaatt ttatctaaag taattttaaa
     2821 cgaaccatta ccaagaaagt atagcgaatt ttattttact ttcattgctc aagatagttt
     2881 tactgctgta ttctcctgga atgaaatttt taactcgcct acaggtaata acctttttat
     2941 tattacccaa aaagatggaa aaagcgtaac agaaatgccc gaatctgttt taatactaac
     3001 tgcaacagat tttacaacag gcagaagata tataaaaaaa ttatctgcca ttatagttag
     3061 cagagctaag taatttctca ttttccttat ttctacaaaa gttttcccaa ttgctacttt
     3121 ccattttagt tagcaataat attttagcac cctaacaaaa tattagacac tttaccaaat
     3181 attaaaatgg ataaaacacc tacaattaat aataatattg ttaagcccga agaaaacttt
     3241 aaagcaatga gcaatattga aaaggctaac aaaaaaggta ttaaaaatgg cttcttttct
     3301 tttcttctgg ttttactctt aatgcccatt ggccatgcaa taatgatttt aaacgaaaaa
     3361 gttttacata accaaaaata tgtaggggct tttattatgg gctttattgg aattggttta
     3421 atagtttggg gaattaaaaa aaataaaaaa gcaaactttg caaccataat ggggttttta
     3481 ggtggtgtgt taatatggac aggctgggtt gagttttctt ttatgtggat tgccgaaaaa
     3541 aataatgttg ccaacttaat ggttaatgga caaattgcca ccaaaagaga atacttggta
     3601 atgctatcct ctttagggtt attaggtacc attatgactt actttttctt tacaagaagt
     3661 aattgtacat tttttatttg gtttcaaaac aagttgggat ataaaaatga tattgcccca
     3721 gagggaaggt attttaaaaa acctttagcc attacaactt ttatagaaac cattatgcta
     3781 ctctggtttt tttatattgt attattagtt gtttatgatg atagtattgc tggagataaa
     3841 catattgcaa cttatattgt tgcatggggc tccttaattt ggtctattta tctaatttct
     3901 aagttaatta ccattcaagt ttttgattat gctgtaagat atgcagtgcc aacagttatt
     3961 attttttgga attttataga agtattaggc agatgggaca cttttaaaga agtttggatt
     4021 taccctattg agcattggtt tgaagtttct ttaatgattg tgctagtaat tggcttatta
     4081 tttctttttc ttaaaacacc ttactttaaa aaagagttaa gaagagttta aataatataa
     4141 ccattattgg taaagagttt gtttataaaa tttatgttag ttttatttac ctctttacca
     4201 attttatgat ttggaagcta tgcttttact tgtttattct tctataattt catttccttt
     4261 gttgtcaata taaaattcta ttccgtcttt ttctactttg gctttgccgt ctttgaacgg
     4321 aaaggcagat tcatacaaaa atggaataac tactttacct tttttgtcaa taaatccaaa
     4381 ttttccattg agttcaacta ctgcaaaacc ttcggaaaaa tcgtctgccc aatcatattt
     4441 aatagatatt acttctttac ctttttggtt tataaagcca tgtttttcgt taatctcaac
     4501 tgctgcaaga ccttcagaaa aaatgttagc atcatcatat ttaagagata ttactacttt
     4561 atctgttttg tcaataaaac cccatttttt attgagactg actggtgcca acccttcaga
     4621 aaatatgtcc accttatcat atttaattgg aataactact ttaccagttt tgtcaataaa
     4681 accccatttt ttattgagta aaacatttgc aaagccttca taaaaattac caacatgctc
     4741 gtatttgggc tgtattgcta cattttcttt ctcctctttg tatctatagt tatcgttttc
     4801 tgattctaac ggctttagat gttgtgcctg cattgttatt acaaataaaa acataatgca
     4861 gaaggtaatg acagctttca taaatgtggt ttttctgtaa atttattatt tattactatt
     4921 tttatcaata ccctttgtaa ggtatttttg tatcacagtt gaaattgtta ttatttttct
     4981 tccttaaaac accgtacttt aaaaaagagt taagaaggtc ttatctaaat aattccaatt
     5041 tacacataac atcatccaac aatactgttg taataatttt ttgttggatt ttgtttttag
     5101 aatatggcat aaacaaaatt gctttggtgt ttaattttat tttaaacttt acaccaacaa
     5161 tatatgacag agttagagaa aaatattatt gcaattaaac gcagaataga agttgcctgt
     5221 ataaatgcaa atagaaatac aaatagtgta aaattattac ttgccaccaa aacggttaat
     5281 gcaaacaaaa taattgaagc tattaattta ggcgaaactt taataggtga aaataaaata
     5341 caagaagtaa aagaaaaatt tgaagcgtta aaaaatattc catatcaaaa acattttatt
     5401 ggctatttac aaagcaataa aataaaagac attttaaaat atgaggttaa ttgtattcaa
     5461 tctttagaca ggctaagcct tgccgaaaaa cttcataaac gcttaacttt agaagaaaaa
     5521 aaattagacg ttttaattca agtaaacacc tcttttgaag ctagtaaatt tggcattgca
     5581 cctaataata caatagattt tattaagcag gttgccaact ttgaaacttt acaaataaaa
     5641 ggtttaatga ctattggctt atttagttca gatgcagata aagtacggca atgttttaaa
     5701 aatcttaaag cattacagca gcaaattatt gcattaaact tacccaatgt atctatgcaa
     5761 gaattatcaa tgggaatgag tggcgacctt gaaattgcta ttgaagaagg tgcaaccatt
     5821 ataagagttg gtactgctat ttttggtaaa agaatatacc cagatagtta ttattggaac
     5881 gaagggtaaa tgtatattta gattgaaact attttattta aatatttttt acatcttttt
     5941 taaaaatcta aaaacactta gtaataacat aataattagt atttaatata taaatatttt
     6001 attaatttaa aaaaactaaa cataaactgt gattaaagtc atagcttagc tatttgccta
     6061 aaaatacttt tgcactccaa agcaaataaa taagcttaac aaaatgaaca tttcctcgtc
     6121 agcaaaaatt gacttaaaaa gttcagtatt aatcatcttc tctttaatat tttcttttag
     6181 tgcaatttcc caaacaaccg attctagtgt aaataattta aaagttcaaa tacagtttag
     6241 gccaaaatta gaagtaagag atggggcatt taggccatta gcaaaaaaag aagaaccggc
     6301 agtattggta agcgaaagaa ttagactaaa cattaattat tcatacaaaa atatttttac
     6361 tttaaaaata tctccacaaa ctgttggcat ttgggggcaa gccaatgcag tacaaggtgt
     6421 agaaaatagt ggtaataaaa ttgcactttt tgaagcttgg accaatttta atttgtctaa
     6481 tacttggaat ataaaattag gtcgccaagt tattagctta gatgatgaaa gattttttgg
     6541 agaattagat tgggctcaag gtggcagaac acacgatgct ttatcttttt tatacaaaaa
     6601 aaataatgtg aatgtaaaaa gttattttgc atttaatcaa aactataaat cattgtacaa
     6661 taacaattct tacaacccag caggcaattt gtacaatagt aacgatgcag taccatataa
     6721 aacaatgcaa actttatggg ttggttttaa tgcaaatgcc atatcaactt ttagtttttt
     6781 atttaccaac ataggtttac aacaagcagc taatttaaat aacaccaatg cagcagtaca
     6841 tcagtctcaa acttttggta tcaattattt taatgctggc aaaactacat ttggcaacct
     6901 gtctgcctat tggcaaactg gtaaaaacat tgctggtact aaaacacaag cctttttatt
     6961 agcaggaagt atagggtata aatttagcaa caaaacaaaa ataatggttg gtactgattg
     7021 ggttagtggt aacgatattg gccaagcaaa accaccaact accaataatg catttacccc
     7081 atactttcat acaggccata aattttatgg caatatggat tattattttg caggcaatgg
     7141 tcatcaaaat gcaggattat cggatagtta tctaaaccta caatgcactc ctaccaaaaa
     7201 attaaatgct actattgctg tgcatcagtt ttatacaact aataaaattt ctagtcagtt
     7261 aaaaaattat agtaaaaact tagggcaaga gcttgatttt tctttacaat ataaacccaa
     7321 tagttttaca agtttatctt gcggttattc attttatgta acaacaccaa ctatacatta
     7381 tttaaaaaac acaactggtt ctaaagatta tcaacaatgg ctatggcttt ctttaaatgt
     7441 aacgccaacc ctattaataa gtaaatttta atttttaata catacatcat gaaactaaaa
     7501 cgtttaattt taattgcaac agcaccattg attgttacaa catacagttg taaaaccaat
     7561 cctaaccaaa caggtagcac taagcctgca attgatatgg ttgcagaagg aaatcctgaa
     7621 gttgcagaag ttacaggagc accaaaagta ccagcaccag taggtaacag aggtcctaaa
     7681 aaactaattg taaatttagt aactacagaa atggaaggaa ttattgcaga cagtattcca
     7741 tacacattct ggacatttaa ccatacagtt cctggtagtt ttattagaat tagagttggc
     7801 gatgaagtaa cattaaactt aaaaaacgaa gccagcagtg tattaccaca taatatagat
     7861 ttacatgcag taacagggcc aggtggtggt gctgcagcta catcttctgc accaggtaaa
     7921 acaacttctt ttacatttaa agcattaaac ccaggtttat atgtttacca ttgtgctgcc
     7981 cctcctgtac ctatgcatat tggtaatggt atgtatggtt taattttagt tgaaccagaa
     8041 ggtggcttac caaaagtgga taaagaattt tacattatgc aaggagattt ttatacaaaa
     8101 gcatcagcta ttaaaaaagg attattagag tttgataatg ataaagcagt tgccgaaaat
     8161 ccggattatg ttttatttaa tgggaaaaaa ggttctttat taggtgccaa catgattgag
     8221 gcaaaagttg gcgaaactgt tcgtttatac gttggtaatg gtggtccaaa ccttacatca
     8281 tcttttcatg ttattggaga aatttttgat aatgtttatt tagaaggtgg tagtactatt
     8341 tctcacaata ttcaaactac tatgatacct gcaggtggtg cagcaattgt agaatttaaa
     8401 tgcgattacc caggagaata tgtgttagtt gatcactctt tatccagagc atttaataaa
     8461 ggagccattg gtaaattaaa agtaactggc aaggcagatg ctaaagtatt taatgcaaaa
     8521 actattggca gccattaata ttttctaaaa aaataattga attgaaaatt aatatctttt
     8581 taataattac tgtttttaca ttactacttt atgcatgtaa taaccagcaa aaaatagatt
     8641 cgaaagaaat taaaattgct aattcaacta cccctaacaa ttcaataccc ttaactgtaa
     8701 aaatggttta tattcccggt ggggagtata aaccatttta tggttctgac tctactttag
     8761 ttaaagtgcc agccttttta ttagatgaaa gggcagttac caaccaggag tttttggcat
     8821 ttattaaatt aaatcctgcc tggcgtaaat ctgttataaa aagaatatat gccgatacaa
     8881 gctatttaaa taattgggtt aatgatacaa cactacccaa agatgctaag cttgatgcac
     8941 ctatcacaaa tatatcttgg tttgcagcaa aagcatttgc aaaaagtatt ggaaaaagat
     9001 tgccaacatt agacgaatgg gaatatgtag caatggctga taaaaccaca gcaaatgcaa
     9061 gaagtaaacc aacttactcc aatgaaattt tagatttata ccttcaaaaa aacaggcaat
     9121 acaatgctgt aaaacaatca gcacctaact attggggcgt atataacatg tttgatttaa
     9181 tttgggagtg gactgatgat tttaattctg tacttacaac aggcgattct agaagtggcc
     9241 aatacgatga taaaaatttg ttttgtgctg caagtgcaac cagtgcaaca gatataatga
     9301 attatgcagc ctttatgcgt tttgctttaa gaggttcggt aaaagcaaat tttgctattg
     9361 ctaatttagg gtttaggtgt gcaaaagaca ctttaatttt aaataaataa ttaaaatgaa
     9421 atactttttt tactgcttaa tttccgttgt tttattttct gcttgtaaag aaaacaaaaa
     9481 acaaactgtt caacttcctt ccgattctat tttctattta acctcacaat ggaaaaacca
     9541 aaatggcgat tctcttcaac taaaagcctt acagggaaaa actttagcag tagtaat
//
LOCUS       DAOQPK010000072         9487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_3856_length_9487_cov_4.395038, whole genome shotgun sequence.
ACCESSION   DAOQPK010000072 DAOQPK010000000
VERSION     DAOQPK010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9487
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3856_length_9487_cov_4.395038"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PLU36_04960"
     CDS             complement(<1..316)
                     /locus_tag="PLU36_04960"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HMZ46132.1"
                     /translation="MKQKETLLNIRLNAENKIELSDMMIDEMGNDHVSTSVDTPIRED
                     AFELSDSEKVKEIEKHFTSIMQILGLDLTDDSLKGTPKRVAKMYVQEIFNGLNPANKP
                     SIT"
     gene            complement(279..689)
                     /locus_tag="PLU36_04965"
     CDS             complement(279..689)
                     /locus_tag="PLU36_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HMZ46133.1"
                     /translation="MAKVAVYRKEHFNAAHRLHNNNWSEEQNKLIFGKCNNENYHGHN
                     YELVVKVIGEPNIDTGFVIDTKELSNLIQKEVIEKFDHKNLNKDIAAFKNIIPTAENI
                     AIEIYNILRPQIETELELQIRLYETERNFVEYPS"
     gene            complement(933..1550)
                     /locus_tag="PLU36_04970"
     CDS             complement(933..1550)
                     /locus_tag="PLU36_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ46134.1"
                     /translation="MFEIILENTGKRFNKEWIFKDLHYVFKSNNFYAIEGANGSGKST
                     LLQIIAGSMLASNGNVKYKTNSTEIASEKVYHHVAIAAPYIEVIEEMTANEFLHFHHS
                     FKQLVLPVPEILQIIALEKAANKQIRYFSSGMKQRIKLAQAFFSNTPVLLLDEPCTNF
                     DAAGFNLYENLLTNYCSNKLVIICSNDENEIKQCHYRLNMLNFKT"
     gene            1874..3592
                     /locus_tag="PLU36_04975"
     CDS             1874..3592
                     /locus_tag="PLU36_04975"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HMZ46135.1"
                     /translation="MPLNINIKKLSRNFLPLEFTVTTWDKLEPYFKNLVDRIINSKED
                     LEQWLLDLSELEAVVSEDACWRQIKMTCDTTDKSLEEAFTFFCMEIQPKIQPYADLLN
                     KKLIHNQFTKQLDQTKYFTYLRGVKKNIDLFREANIPIQAELSVLQQQYGAIAGKMTV
                     EFEGKEYTLQQASKFLESKDRSVRETMYKKIQERRLQDANELQNLFTILISKRNTIAI
                     NAGFKNYRDFKFVDLGRFDYTKEDCYQFHEAVKQHILPLVNKIYAKKQAQLGLTTLQP
                     WDTEAEPIGVEPLKPFKTAKDLYEKTVQCFEQLDPFFANCLRQMNELKHFDLESRKGK
                     APGGYNCPLAESGAPFIFMNATGQMSDVTTMVHEGGHAIHSFLSHHLELCAFKEYPME
                     IAEVASMAMELFSMDYWHSFFDNEAELKRAKEHQLERTITIFPWIATIDKFQHWIYEN
                     PTHSEEERTNKWMEIVQEFSSDVIDFTGLDEYRKISWQKQLHLFEVPFYYIEYGIAQL
                     GAIGLWMQFKQNKKQALNNYTNALSLGGTKTLPELYNAAGLQFNFTPAYIQQLMDFVS
                     NEMEHL"
     gene            complement(3596..4339)
                     /locus_tag="PLU36_04980"
     CDS             complement(3596..4339)
                     /locus_tag="PLU36_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HMZ46136.1"
                     /translation="MLKAIIVDDEAKARRILENFITEYCPQIQICALVEDVPQAVKAI
                     KKHEPDVVFLDIEMPGYNGFQLFEFFDEVNFEIIFITAYSEFALKAFQVSAIDYLLKP
                     LQIDQLINAVKKLETKANKATLKNRVEALQKNIQEQVIKKIVVPFSNGSLFLETSEIT
                     HLKAEGSYVNIFMKDGSKILVSKNIKDYEEILTANEGFFRTHRSFLLNKNYIVKLNSD
                     STEAVLTTGNSILIARERKQDFLQFIKNR"
     gene            complement(4493..6277)
                     /locus_tag="PLU36_04985"
     CDS             complement(4493..6277)
                     /locus_tag="PLU36_04985"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ46137.1"
                     /translation="MAQKPIVSFSFDNTLNDTTNTFAFTPSTALTGSGWGFATNRFGE
                     ANKAFACTNNSNRSLTANITSLPQGNSARTIEFWYKFNSSSSPNFLVAYGSNITSQGY
                     GITQESNKIINYGWANDIEVAQTFTVGVWYHYAIVYDGVTAYIYRNGLFLTSAAKYWN
                     TIGTNLLIGSTPSLLKSFNGTIDDLRIYDVELGLPSIQYLYNNAKNLPSPPYKWNFNN
                     SYSDTTNQMSFSSNAGTSFVNDRFGNANSAININNTGCTATIFGLPYHKKPRSISFWV
                     KHNVVIGSPMFNMWYSYGKNGTNYANGGGYHQLDGIYHFGYLNNHATTISSPLLPNQW
                     YHIVFVFDGTLSKIYSNGTLLSSQTMTGWNTLGIDDVFKLGIGIGHEISFNGAIDDLR
                     IMNKALTDAEVSALYASESTLPMQLHQFSAQLKNNTTLLKWSTATEVNTSHFEIEYSN
                     NGKEFTTVGTVAAKGNSSNLNAYNFTHNVNNQSMHYYRLKMIDNDAKFEYSNIVKLKT
                     TNKFFDAIVYPTIANNTITASITAKEKANATIELFGMNGNKVLQKTVSFVEGSQTYNI
                     NIAHLIKGSYIVSIKAGNNVQSSRIIIQ"
     gene            complement(6427..9465)
                     /locus_tag="PLU36_04990"
     CDS             complement(6427..9465)
                     /locus_tag="PLU36_04990"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HMZ46138.1"
                     /translation="MIVVKHCIFFFICFYAITAKAQIPTYIHYEVNDGLPSNEIYSLK
                     QDKNGFLWIGTEGGLAKYNGRNFILYPHKNLKGLGITGIQEDAFGKVWCYNFSGQILH
                     TQNDSLVVFKPWANFYKEQFADFALDNNNQIFVSNFKNNVYVFNIKYLNLVNSLSNNT
                     TIKQGISVTHDNKIIFTNLTDGKPYIIEQNLLIPVPLKAWQNLPADAKAENTLVFFKS
                     SKNLQTFAIQRLHPNIPFPSLYQFVHNQLQIHPVTAYLRKNKIHPLSVLNDDEGNLFI
                     GTFNGCLWFKQQGENWNFHKNIFENNAVSAIIKDRENSYWFATLKNGLYQVPDLAFQI
                     FTKEDIGILETEIGDVETDGKSKLFVSGSTNELFTINILNNKLVHKNFTKDKRYAQAF
                     YYDKLFNRLLLYKSEVSILENNILKNISTLTSAAKNFYVRKDGVIFIAGSSLDAIFLT
                     SNKNLTLTSEFGEVEKSNTELNKHNSNIWGRVVISTQRSRDVWYNEKDKILWSANSDG
                     VVYYKNKKVNTLFDTKSNKQIIATCFQNLENNLLCIGTVDQGIYIIQDTSIVLHYTTK
                     NGLQSNQINKIKADEQNIWAITDKGIQRFNLSTNNFINISKSDGLISNQVFDMAIANN
                     TVYISTSKGLQFLPVNAIKTSNQLPFLGIKKVSINDSIYNYKNTILLNSKNGNIEIEL
                     EYALLKNSGNIALKYRMIGLDTLWIEDKAFNNIIRYNTLSSGSYKFEAVCMNDDGVES
                     KPVTIHFTVLKPWWLQWWFLITAACISILLVIFFTKLYLNISKNKIAEELKKSKLQEE
                     ARQSQLASLKAQMNPHFMFNALNSIQEYILLSDKRLANMYLGKFADLMRLILDQSNKE
                     IISLVEELKILNLYLELEALRFEENFEYIIQKENIEDEAEINIPAMLIQPYVENAIKH
                     GLLHKQGLKKISIHFKLQKNKLLCVVEDNGIGRKRSGEINALRNKKYTSFATGATQKR
                     LELLNQNNPNLIVVNYTDLTLNNTTYIGTKVSIVIPIIEL"
BASE COUNT         3199 a         1416 c         1318 g         3554 t
ORIGIN      
        1 aagtaataga aggtttattg gcaggattta aaccattaaa aatttcttgt acatacattt
       61 tggctactct tttaggggtt ccttttaagc tatcatcagt taaatctaag cctaatattt
      121 gcataatact ggtaaagtgc ttttcaattt cttttacttt ttcactatcc gataattcaa
      181 aagcatcttc tctaataggt gtatccacac ttgtacttac atggtcgtta cccatttcgt
      241 ctatcatcat atcgctaagc tctattttat tttctgcatt aagacggata ttcaacaaag
      301 tttctttctg tttcatataa tctaatttgt agctctaatt ctgtttcaat ttgcggtcta
      361 agtatattat aaatttcaat ggcaatattt tcggctgttg gaattatgtt tttaaacgct
      421 gctatatctt tattcaaatt tttgtgatca aacttttcaa taacttcttt ttgaattaaa
      481 ttactaagct ctttagtgtc tatcacaaat cctgtatcaa tattaggttc tccaattact
      541 ttaaccacta attcataatt gtggccatga taattttcat tattacactt gccaaaaatt
      601 aatttatttt gctcctcact ccaattgtta ttatgtagcc tgtgtgcagc attaaaatgt
      661 tcttttctgt aaacagctac tttagccata ttttatttat tagatatttt tataactatc
      721 aacaaagttt tataatgcat cttgctttgt taataataaa tactaagttt ttaaaaataa
      781 tatttgaaaa atgggttaga tgtttactca aacaaaagtg tgttgtttaa agcaaaaatc
      841 ttttagttaa ttgtcctcaa tcatttacaa atatatacac atatatgagt aaggaaggta
      901 taaaaaggtt acaaaatttt taaaattttt atttaggtct taaaattcaa catatttaac
      961 ctgtagtggc attgttttat ttcattttca tcgttactac aaataattac taatttatta
     1021 gagcaataat ttgttaatag gttttcgtat aaattaaacc ctgctgcatc aaaatttgta
     1081 caaggttcat ctaacagcaa aacaggagtg ttagaaaaaa aagcctgggc caatttaatt
     1141 ctttgtttca tgcccgatga aaaatatctt atttgcttat tggctgcttt ttctaatgct
     1201 attatttgta aaatttctgg tactggtaat actaattgtt taaaactgtg atgaaagtgt
     1261 aaaaactcgt tagccgtcat ttcttctatt acttcaatat aaggagcagc aattgctaca
     1321 tggtggtaca ctttttcgga agcaatttct gttgaattag ttttatattt tacgttacca
     1381 ttacttgcca acatacttcc tgctattatt tgcagcaaag tgctttttcc actaccattt
     1441 gccccttcaa tggcataaaa attattgctt ttaaatacat aatgaagatc tttaaaaatc
     1501 cattctttat taaatctttt tcctgtattt tctaaaatta tttcaaacat ttttaactgt
     1561 gtaaattagt tgtagtatat aattttataa ttcttattta cacttgcaat ggaactataa
     1621 ataatatatt gatgcaaatg tagtataaga taatgaagca ataaaaggta actatttatt
     1681 aaaatttata gggtgttacc tattttaata cctataataa agcatatttt ttgggattta
     1741 tttgctaaaa aatatttatt tatttgtata tctatattaa tactaacact ataaaaagtt
     1801 ttaacaaaga actagtacca acaaccaaaa cttttaatat cacccactac atttgcagca
     1861 ataaaatttt acgatgccat taaatataaa cataaaaaaa ttatcaagaa attttttacc
     1921 tcttgaattt actgttacta cttgggataa gcttgaacct tattttaaaa atttagtaga
     1981 tagaataatt aatagtaaag aagatttaga gcaatggctt ttagatttga gtgaattaga
     2041 agcagtagta agtgaagatg cttgttggcg tcaaataaaa atgacttgcg atactactga
     2101 taaaagctta gaagaagcat ttactttttt ttgtatggag attcaaccaa aaattcaacc
     2161 ttatgccgac ttacttaata aaaaacttat ccataaccaa tttactaaac aattagacca
     2221 aacaaaatat tttacttact tacgtggggt taaaaaaaat atagatttat ttagagaagc
     2281 aaatattcct attcaggcag aattaagtgt tttgcaacaa caatatggag ctattgcagg
     2341 caaaatgact gtagagtttg agggtaaaga atataccttg caacaagcat ctaagttctt
     2401 agaaagtaaa gacagaagcg taagagaaac gatgtataaa aaaattcagg aacgtcgttt
     2461 gcaagatgct aatgagttgc aaaatttatt taccattctt atttcaaaaa ggaatacaat
     2521 tgcaattaat gctggtttta aaaattatag agattttaaa tttgtagatc ttggaagatt
     2581 tgattatact aaagaagact gctaccaatt tcacgaagca gtgaaacaac atattttgcc
     2641 tttggtgaat aagatttatg caaaaaaaca ggcacagctt ggtttaacaa ctctacaacc
     2701 atgggataca gaagctgagc caataggcgt agaaccatta aaaccattta aaacagctaa
     2761 ggacttgtac gaaaaaacag tacagtgttt tgaacaactt gatccttttt ttgctaattg
     2821 tttacgccaa atgaatgagc ttaaacattt tgatttagaa agtagaaaag gaaaagctcc
     2881 aggtggttat aactgcccat tggcagaaag tggagcacca tttattttta tgaatgctac
     2941 tgggcaaatg agtgatgtaa ctacaatggt acacgaaggt gggcatgcca ttcattcttt
     3001 tttaagccac catttagaat tatgtgcttt taaagaatac ccaatggaaa ttgcagaagt
     3061 tgctagtatg gcaatggagc tttttagcat ggattattgg catagctttt ttgataatga
     3121 agctgaatta aaaagagcta aagaacatca gctagaaaga actataacta tttttccgtg
     3181 gatagcaacg attgataaat ttcaacattg gatttacgaa aacccaacac attcagaaga
     3241 ggagagaact aacaaatgga tggaaattgt acaagaattt tcatcggatg taatagactt
     3301 tacaggttta gatgaatata gaaaaataag ttggcaaaaa caattgcatt tattcgaagt
     3361 gcctttttat tatattgagt atggcatagc acaattaggt gctattggtt tgtggatgca
     3421 gtttaaacaa aataaaaaac aagcattaaa taattatacc aatgcattaa gcttaggggg
     3481 cacaaaaact ttacctgagt tatataatgc agcaggttta caatttaact ttacacctgc
     3541 ttatatacaa caactaatgg atttcgtaag taacgaaatg gagcatttat aaatgttatc
     3601 tattttttat aaactgtaaa aaatcttgct ttctttctcg ggcaattaaa atgctgtttc
     3661 ctgtagttag aacagcttct gtagagtctg agtttagttt tactatataa tttttattta
     3721 gtaaaaaact cctgtgtgtt ctaaagaaac cttcgtttgc agttaatatc tcttcgtaat
     3781 cttttatatt ttttgatact aatattttac tgccatcttt catgaaaata tttacatagc
     3841 taccttctgc ttttaaatga gtaatttcac tcgtttctaa aaacaaactg ccattgctaa
     3901 aaggaactac aattttttta attacctgct cttgtatatt tttttgtaat gcttccactc
     3961 tgttttttaa agtggcttta ttggctttgg tttctaattt ttttacagca tttattaact
     4021 gatctatttg taatggtttt aataaataat ctatagccga aacctgaaaa gcttttaaag
     4081 caaattcgct ataagcggtg ataaaaataa tttcaaaatt tacttcatca aaaaattcaa
     4141 acagctgaaa cccattgtaa ccaggcattt caatatctaa aaacactaca tctggttcgt
     4201 gttttttaat tgcttttaca gcttgaggca catcttctac taatgcacaa atttgtattt
     4261 gtgggcaata ctctgtaata aaattttcta aaattcttct agctttagcc tcatcatcta
     4321 caattattgc ttttagcatt atgcattaat tttttacaaa ttaagtttaa tatatattaa
     4381 aaaacttgtt ttgcatttca aaaaattaaa ggcaatattg gaacgcctga atgaataaat
     4441 cccccaattt attgttcaag cattccttgt ttgccaagat ggttattatt tattattgta
     4501 taattattct gctagattgt acattgttac cagctttaat gcttactata taactacctt
     4561 ttataagatg tgcaatattg atgttgtatg tttgacttcc ttcaacaaat gaaacagttt
     4621 tttgtaatac tttattccca ttcattccaa acagttctat agttgcattt gctttttctt
     4681 tagcagtaat gcttgctgta attgtattat ttgctattgt tggataaact attgcatcaa
     4741 aaaatttatt ggtagttttt aattttacta tattactgta ttcaaatttt gcatcattat
     4801 ctatcatttt taacctgtag tagtgcatgg attgattgtt tacattgtgt gtaaaattgt
     4861 atgcatttaa gttagaacta ttgccttttg ctgcaacagt tcctactgta gtaaactctt
     4921 tcccattatt actatattca atttcaaaat ggctagtatt tacttcggta gcagtgctcc
     4981 attttaataa tgtagtattg ttttttagtt gagcagagaa ttgatgtaac tgcatgggca
     5041 aggtactttc actagcatat aatgcactta cttctgcatc tgttaatgct ttgttcatta
     5101 tccttaaatc atctatagct ccattaaaag atatttcatg acctatacct atacctaatt
     5161 taaatacatc atcaatccct agtgtattcc aacctgtcat tgtttgcgat gacaaaagtg
     5221 taccattgct atagattttt gacaaagtgc catcaaaaac aaaaacaata tgataccact
     5281 ggttgggcaa taaagggctt gatattgttg ttgcatgatt atttaagtat ccaaaatgat
     5341 aaatgccatc tagttggtga tatccacctc catttgcata atttgttcca ttttttccat
     5401 aactatacca catattaaac ataggactgc ctattacaac attatgttta acccaaaaag
     5461 agatagacct tggtttttta tgatatggta aaccaaatat tgttgcagta caacctgtat
     5521 tattaatatt tattgcacta tttgcattac caaacctatc gtttacaaaa ctggttcctg
     5581 catttgaaga aaaagacatt tggttagttg tatcgctata agaattatta aaattccatt
     5641 tatatggcgg tgatggtaaa ttttttgcat tgttgtataa gtattgaata gaaggaagtc
     5701 ctaattctac atcataaatt cttaaatcat ctattgtacc attaaagctc tttaataagc
     5761 ttggggtaga gccaattaat aaattagtgc caattgtatt ccaatatttt gcagcactgg
     5821 ttaagaacaa tccatttcta taaatataag cagttacacc atcatatact attgcatagt
     5881 ggtaccaaac acctacagta aaggtttgag caacttcaat atcatttgcc caaccgtaat
     5941 taattatttt attactttct tgagtaattc cataaccctg actagtaata ttactaccat
     6001 aagctaccaa aaaatttggg gaagaacttg agttaaactt ataccaaaac tctattgttc
     6061 ttgccgaatt tccttgtggc aatgaagtaa tattagccgt taaagatcta ttggagttat
     6121 tagtacatgc aaaagcctta ttggcttctc caaacctatt tgttgcaaag ccccaaccac
     6181 tacctgttaa agcagtacta ggtgtaaatg caaaggtatt agttgtgtca tttaatgtat
     6241 tatcaaatga aaagctaaca attggctttt gtgccattaa cgaaatagtt gtaaagagaa
     6301 aaatagtaaa tacaagtgta aatttttgtg tcataaatta ttaaattatt aacacaaatg
     6361 tttattgact tatttaaaca atgaactaaa ctttgtgggc tgtataaatt gttttgtaaa
     6421 ctgtaattat aattcaataa ttggtattac aatactcact ttagtaccaa tataggttgt
     6481 attgtttaat gttaaatcgg tataattaac tacaattaaa tttggattat tttggttaag
     6541 aagttctaat cttttttgtg tggcaccagt tgcaaaagaa gtatattttt tatttcttaa
     6601 cgcattaatt tctcctgacc gttttctacc aataccatta tcctcaacaa cacatagtag
     6661 tttatttttt tgcaatttaa aatggatact tatttttttc aacccttgct tatgtaataa
     6721 cccatgtttt attgcatttt caacataagg ctgtattagc attgcaggaa tattaatttc
     6781 agcttcatct tcaatatttt ctttctgaat aatgtattca aaattttcct caaaacgcaa
     6841 tgcttctaat tctaaataaa ggtttaaaat tttcaattct tccactaaag aaataatttc
     6901 tttattgctt tggtctaaaa tcaatcgcat taaatctgca aacttgccca agtacatatt
     6961 ggctaacctt ttatcgctaa gcaaaatata ttcttgaatt gaatttaatg cattaaacat
     7021 aaaatggggg ttcatttgag cttttaagga tgccaactgc gattgcctgg cttcttcctg
     7081 caactttgat ttttttagtt cttcggcaat tttattttta gaaatattaa gatataattt
     7141 tgtgaaaaat attaccaaca ggatagaaat acatgcagca gtaattaaaa accaccattg
     7201 taaccaccaa ggctttagaa cagtaaaatg tattgttact ggtttacttt caaccccatc
     7261 gtcattcata caaactgctt caaatttgta acttccagaa gataaagtgt tgtacctgat
     7321 aatattgtta aatgctttat cttctatcca taaagtatct aaaccaatca tcctatattt
     7381 aagtgcaata tttccagagt tttttaataa tgcatattct aattcaattt caatatttcc
     7441 atttttacta tttagtagta tagtgttctt ataattataa atactatcat taatgcttac
     7501 ttttttaatt cctaaaaatg gcaattgatt acttgtttta attgcattaa caggtaaaaa
     7561 ttgtaggcct ttagaggtag atatataaac tgtattattg gcaattgcca tatcaaaaac
     7621 ttggtttgaa ataagcccat ctgattttga tatattgata aaattgtttg tagaaagatt
     7681 aaatcgttga atgcctttat ctgtaattgc ccagatattt tgttcatccg cttttatttt
     7741 attaatttgg ttagactgca acccattttt agttgtataa tgtaaaacaa tggaggtgtc
     7801 ttgtattata taaatacctt ggtccacagt gccaatgcat aataaattat tctctaaatt
     7861 ttgaaaacaa gtagcaataa tttgtttatt tgatttagta tcaaataatg tatttacttt
     7921 tttattttta taataaacaa ctccatctga atttgccgac cataaaattt tatctttttc
     7981 attgtaccaa acatctctag atctttgtgt tgaaataact actctgcccc aaatattgct
     8041 attatgtttg tttaattctg tattgctttt ttcaacttct ccaaactcac ttgttagggt
     8101 taaattttta tttgaggtta aaaatattgc atctaaggaa gagcctgcaa taaaaattac
     8161 accatctttt cttacataaa agtttttagc agcactggtt aaagtgctga tatttttaag
     8221 aatattattt tctaaaattg aaacttccga tttatataaa agcaacctat taaataactt
     8281 atcgtaataa aatgcctgtg catacctttt atctttagtg aaatttttat gaactaactt
     8341 gttatttaag atgttgatgg taaatagttc attggtagat cctgatacaa ataatttact
     8401 cttgccatca gtttctacat cgccaatttc ggtttctaaa attccaatat cttcttttgt
     8461 aaatatttga aatgccaaat caggaacctg atataatcca ttttttaatg tagcaaacca
     8521 ataactattt tctctgtctt ttataatggc agatactgca ttgttctcga aaatattttt
     8581 atgaaaattc cagttttcgc cttgttgttt aaaccataaa caaccattaa atgttccaat
     8641 aaataaattt ccctcgtcgt catttaatac acttaaagga tgaattttat tttttcttag
     8701 ataggcagta acaggatgaa tttgaagttg attatgtaca aattgatata aagaagggaa
     8761 ggggatgtta ggatgtagcc tttgaatagc aaaggtttga aggtttttgg atgatttgaa
     8821 aaacaccaga gtattctcag ccttagcatc cgctggtagg ttttgccaag cctttagtgg
     8881 aacaggaatt aataaattct gttcaattat atatggcttt ccatcggtaa gattggtaaa
     8941 aataatttta ttatcatgtg taacactaat accttgtttt atggtagtat tatttgataa
     9001 gctatttact aggtttaaat atttaatatt aaaaacatac acattattct tgaaattaga
     9061 aacgaaaatt tggttattat tatctaaagc aaaatctgca aactgctctt tataaaaatt
     9121 tgcccaaggt ttaaatacaa ctaaagaatc gttttgtgta tgcaatattt gcccagaaaa
     9181 attataacac cacacttttc caaaagcatc ttcttgaata cctgttatac ctaagccttt
     9241 taaatttttg tgaggatata agataaaatt tcttccatta tattttgcta aaccaccttc
     9301 tgtacctatc cataaaaagc catttttgtc ttgctttaaa ctataaattt cattggatgg
     9361 taagccatca tttacttcat aatgtatata ggtaggtatt tgtgctttag ctgttatagc
     9421 ataaaagcaa ataaaaaaaa atatacaatg ctttacaaca atcacaaaat attatggttt
     9481 atatttt
//
LOCUS       DAOQPK010000073         9380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_3934_length_9380_cov_4.923432, whole genome shotgun sequence.
ACCESSION   DAOQPK010000073 DAOQPK010000000
VERSION     DAOQPK010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9380
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3934_length_9380_cov_4.923432"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PLU36_04995"
     CDS             complement(<1..543)
                     /locus_tag="PLU36_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HMZ46139.1"
                     /translation="MQDYLYGLNEPQKEAVLHINGPLMIIAGAGSGKTKVLTTRIANL
                     MANGVDAFNILALTFTNKAAAEMKERIERILGNNEARNLYIGTFHSVFARILRAEAGK
                     IGYTNNFTIYDTDESRSVLKTIITEMELDVKHYKPNFIHNRISAAKNALISADEYEQN
                     LYLTKEDEKANRPAIAKIYKA"
     gene            727..1743
                     /gene="recA"
                     /locus_tag="PLU36_05000"
     CDS             727..1743
                     /gene="recA"
                     /locus_tag="PLU36_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HMZ46140.1"
                     /translation="MSNVEKLKALKLTIDKIDKDFGKGSVMMMSDRADRQIEVISTGS
                     IGLDAALGVGGVPKGRIVEIYGPESSGKTTVATHIIAEAQKKGGICAIIDAEHAFDSS
                     YAQKLGVDVESLLISQPDYGEQALEIADRLILSGALDVVVIDSVAALVPKGELEGEMG
                     DSKMGLQARLMSQALRKLTATINKTNTICIFINQLREKIGVMFGNPETTTGGNALKFY
                     ASVRLDIRRAAQIKDGDSAIGNHVKVKVVKNKVAPPFRSAEFDIIFGEGISKIGEIID
                     MGVELGIVNKSGSWFSYNSEKLGQGRDAVKQLLLDNAELANEIEAKIRAKLAELQSTA
                     TVEA"
     gene            1880..2485
                     /locus_tag="PLU36_05005"
     CDS             1880..2485
                     /locus_tag="PLU36_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46141.1"
                     /translation="MKKLFILSALFAVISFTATAQVKIKLEPAAAFGKKAISALKTQG
                     TQQTMKAVAEGEECGAFVATKKDDKGANYTEAKEFLIVSNDGVTGFAFTMYATTYEAK
                     TLYVVRGMPLETGVCVDKSTKVTFTFEDGTQRTFTNFEKDNCKGIVTLYMHELVKNMD
                     AYEDLKTKKVRSIRLEGIEKTVVKSLSEGNQQQLQGTIKCL"
     gene            complement(2551..3828)
                     /gene="tyrS"
                     /locus_tag="PLU36_05010"
     CDS             complement(2551..3828)
                     /gene="tyrS"
                     /locus_tag="PLU36_05010"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HMZ46142.1"
                     /translation="MNLIEELRWRGMIADIMPGTEEQLVKEMTTGYVGFDPTAESLHI
                     GSLVPIILLVHMQKSGHKPMALIGGATGMIGDPTGKSDERNLLSEETLQKNIEGIQSQ
                     LKQFISFDDEKPNAAILVNNYDWFKNISFIDFLRDTGKHITINYMMAKDSVKKRIEGE
                     NGISYTEFAYQLMQGYDFYWLYKNKNCKLQFGGSDQWGNMTTGSELIRRMCGGEAFVF
                     TCPLITKADGGKFGKTEGGNVWLSADKTTPYQFYQFWLNTSDADAEKWIKIFTFLTKE
                     EINSLIIQHQQNASDRVLQKTLAKEVTIFVHGENEYEKAVETTQKLFANANAPIESLS
                     VDDLENMEGVQKIKLDLHNLKQGMDIISFLADSSILASKGEARKMLQNGGISINRQKI
                     SDVQFIVQNSLLLHQKYLLVQKGKKNYYLVSFE"
     gene            4057..8928
                     /locus_tag="PLU36_05015"
     CDS             4057..8928
                     /locus_tag="PLU36_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HMZ46143.1"
                     /translation="MKHLLLTFFIVCFGNSIIAQPLHNEWIDYSKTYYKFKVDFGIHP
                     VTLQPIKRKLVRITKATLDSVGLGNTNVEQFKLFRNGVEVPIFTSTTSGVLDSLGYIE
                     FWGEINDGKLDKELYRDADYQLSDIWSLQEDNGTYFLTTSNAINKRLVNTANNVIGNL
                     LSPTLYFMNTVMYTQRQRRFEGYAAQSSLPLHSSSYDKGEGYATRPIRPIGSSCGQVS
                     FTINFTNLKPYLSGPPMTLKVNTMGSENNTRTILVKINGNSVSNYQMDYFNYAKVEES
                     GIPVSYISSGNAAVLHINQSPVDCDEFRLIKDELTYPRTLDGDNNVSLELKLPATATG
                     HYLKFFNFNSGANSPILYDITNGKRYIADKTINDTLQFVTNPSLTAYNLVLVNNDSAN
                     IFSVSTLQQRNFVNYQNIANQGDYIIISNPLIYGTGNSNYVEQYKQYRSSTKGGSYNA
                     IVVDINEITDQFAFGINKHPLSVRNFLRYARNIFTTTPKYVFIIGKGLNYIDFRSNED
                     EPDMPYQNLVPTWGHPASDNLLSAADNLNPTPITPIGRLSAVSANEVGDYLKKIKQYD
                     SLQQAPLVNIEDKTWMKNVLHIGGATDYNIGTSITNYLNKYTQIIEDTLFGAKVKKYN
                     KLDNPAGYGAALVDFKNTYESGASIITYFGHSSANLLDFNLDNPNAYNNPNKYPVFIV
                     NGCDAGNFLTYEPQRLNIKTSISEKFVLAPERGAIGYLATTGYGAINYLDTFTTGFYR
                     ALSTIDYNKPFGESIKDGIINVLNTTGLNDFFARYHAEQFIFHGDPALKLNTFPLPDL
                     VLKTEYTTTLPTKITVAKDSFYLKMDIYNIGKKINDSINLKITRQYPKGNSEVVLNKK
                     LPAIALMTTETAAFPIIANRDKGTTILTATIDATNSINEMDENNNTAVISVSITDDEI
                     VPIYPYNYAIVNNSNFKLAASTANPLDTLKNYIVEVDTTSLFNSPIKYTQNKSSVGGV
                     VEFDYGLTLQNNTTYFWRVATQGSSYWNTVSFTYKSSPDIGFEQRHFLQHTDSKLTDI
                     ILDSSSRKFKFGQTLNNLFVVHSIYPYSGTEDQHFSIKVNGTGIIASACLGNSVIINV
                     FDTLSFNPWINTTNPFGATPTCLETRKYNFEYSYLDVSGRNNAKSFLESIPNGMLVAV
                     RMVYDGASVWANDWANDTLLNGSGNSLYHFLKNQGFAIDSFNHPRTFAFIFKKNDSLH
                     FTPKYLFSEGLYDRVILNADYNSDDTLGYVVSPKFGPAKAWKFAKWNGNGNGSSFAKM
                     NIIGIDTNGIENVLFNNMDTLQTSFDISSINAQQYPFVKLSILNQDSVKAFPYQLSNW
                     SVEYVGVPEGAVAPNLHINIPDTIGLTSSYASDTLKGSFAFKNVSKENFDSLTVKVVL
                     TNLRLGTVHTFNLAKTKPLLAGDTLHINFAIDASLMSRDEYNLYVAVNENAAQPEQYL
                     FNNFLYKYIYFKTSVIVPVKLLNLTAKINGTGTDITWQVAEEVNVLKYEVEHSNNAID
                     FKTIGSVDANGKNNYLLHHAQPTYGKNYYRLKILNTDGSYNYSTIRLVNFGKGVIVNV
                     YPNPVIGKLNIAVSNNNNKQVQVNLYNNYGQLLFTEEFKNNSTIDMSKLSSGVYILQV
                     NDGIQWQTFRIVK"
     gene            complement(9002..>9380)
                     /locus_tag="PLU36_05020"
     CDS             complement(9002..>9380)
                     /locus_tag="PLU36_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HMZ46144.1"
                     /translation="KDLSKYKMDAEYYNIPEGAVQIVNKAKDNGHRVCAIGATTMRAL
                     ETNFTAQKRLKSSEGWSNHFIHPPYDFSIADSMVTNFHLPKTSLFIMTCAFAGYDLTM
                     EAYHKAIKDKYRFYSYGDVLLII"
BASE COUNT         3335 a         1379 c         1467 g         3199 t
ORIGIN      
        1 agctttataa attttagcta tggcaggtct attggctttt tcatcttctt ttgtaaggta
       61 taaattttgt tcatattcat cggcactaat taatgcattt ttagcagccg atattctatt
      121 atgtataaag ttgggtttgt aatgttttac atctaattcc atttcggtaa tgatggtttt
      181 taaaacactt ctactttcat cggtatcgta aatagtaaaa ttattcgtat aaccaatttt
      241 tcctgcttcg gctcttaaaa ttcttgcaaa aacactatga aaagtaccaa tgtataaatt
      301 tctggcttcg ttgtttccta aaattctttc aattctttct ttcatttcgg cagcagcttt
      361 gttggtaaaa gttaatgcca aaatattaaa agcatctaca ccgtttgcca ttaaattagc
      421 aatacgtgta gtaagaactt ttgttttacc actacctgca ccagcaataa tcattaaagg
      481 cccattaatg tgtaatactg cttctttttg tggctcgttt aagccatata aataatcctg
      541 catggctgca atttatagga agtaaaattg gaaattagct ttaggggata agtttagtaa
      601 tttaaaactg cataaaattg gtttgttcat aattttagca aaaaatacta aaaaatttag
      661 tgttaataat aaaaactact tacttttaca gtgtaaacta attttaaccg ataaaaattt
      721 atagtaatga gtaatgtaga aaaattaaaa gcactgaaat taactattga taaaatagat
      781 aaagattttg gtaaaggaag tgttatgatg atgagtgaca gggcagacag gcaaatagaa
      841 gtgatcagta caggctcaat aggtttagat gctgctttgg gcgttggtgg tgtacctaaa
      901 ggaagaattg tagaaattta tggcccagaa agtagtggta aaacaaccgt tgcaacacat
      961 attatagcag aagctcaaaa gaaaggtggt atttgtgcta ttattgatgc tgaacatgct
     1021 tttgatagtt cttatgctca aaaattaggg gtagatgttg aaagtttatt aatctctcag
     1081 ccagattacg gagaacaggc tttagaaatt gcagacagat taatactttc tggtgcatta
     1141 gatgtggtag tgatagactc tgtagctgca ttagtaccca aaggagaatt agaaggtgaa
     1201 atgggtgata gtaaaatggg tttacaggct cgtttaatga gccaggcttt acgtaaatta
     1261 acagccacca taaacaaaac aaatactatt tgtattttca ttaatcagct tcgtgaaaaa
     1321 attggcgtaa tgtttggtaa tccagaaact acaactggtg gtaatgcatt aaaattttat
     1381 gcctctgtac gtttagatat tagaagagca gcacaaataa aagatggcga ctctgcaata
     1441 ggcaatcatg taaaagtaaa agttgtaaaa aataaagtag cacctccttt tagaagtgca
     1501 gagtttgata ttatttttgg agaaggtatt agtaaaatag gagaaataat agatatgggt
     1561 gtggaattag gtattgtaaa taaaagtggt agttggttta gctataatag tgaaaagtta
     1621 gggcagggta gagatgctgt aaagcaactt ttattagata atgcagaatt ggcaaacgaa
     1681 attgaagcca aaattagagc caaattagcc gagctgcaat ctactgcaac tgtagaagca
     1741 taatagaaaa ctttatatct aaaaaattaa atgttaaata acgttaataa aatataaata
     1801 ccttattttg ggtattgtat ttgattaagt acccaattat ttttacatta aaattattaa
     1861 aacacaaaat ttaaaaaaca tgaaaaaatt atttatcctt tcagcattat ttgcagtaat
     1921 atcttttact gcaactgcac aagttaaaat taaattagaa cctgctgcag catttggtaa
     1981 aaaagcaata tctgcattaa aaactcaagg cacacaacaa accatgaagg ctgttgcaga
     2041 aggtgaagag tgtggtgctt ttgttgctac caaaaaagac gataaaggag ccaattacac
     2101 ggaagctaaa gagtttttaa ttgtatctaa cgatggagta actggttttg catttacaat
     2161 gtacgcaact acttacgaag ctaaaacact ttatgttgta agaggtatgc ctttagaaac
     2221 tggagtttgt gttgataaat ctactaaagt tacttttact tttgaagatg gtacacaaag
     2281 aacttttact aattttgaga aagataattg taaaggtatt gttactttat atatgcatga
     2341 acttgtaaaa aatatggatg cttacgaaga tttaaaaact aaaaaagtaa gaagtattag
     2401 attagaaggt attgaaaaaa ctgttgttaa atctttaagc gaaggtaatc aacaacaatt
     2461 acaaggtact attaaatgtt tataattgta agcaacttta tttaaaaatt aagggctgca
     2521 ttaaaaatgc agcccttgtt ttttagcaac ttattcaaac gaaactagat agtaattttt
     2581 tttgcctttt tgcaccaata aatatttttg atgtaatagt aaactgtttt gtacaataaa
     2641 ttgcacatcg cttatttttt gcctgttaat gctaatacca ccattttgca acatttttct
     2701 agcttctcct ttactagcta aaatagaaga atctgctaaa aaagaaatta tatccattcc
     2761 ttgttttaag ttatgtaagt caagttttat tttttgcaca ccttccatat tctctaaatc
     2821 atctacactt aaactttcta taggagcatt ggcattagca aataattttt gagttgtttc
     2881 aacagctttt tcatattcat tttctccgtg aacaaaaatg gttacttctt tagccaatgt
     2941 tttttgtaaa actctatccg atgcattttg ttgatgctgt ataattaaac tattaatttc
     3001 ttctttagtt aaaaatgtaa aaattttaat ccatttttct gcatcggcat cgcttgtgtt
     3061 taaccaaaat tgataaaact gatagggtgt agttttatcg gcacttaacc aaacatttcc
     3121 tccttctgtt tttccaaatt ttcctccatc ggctttggtt attagcggac atgtaaatac
     3181 aaaagcttct ccaccacaca ttcttctaat taactcagag ccagttgtca tgtttcccca
     3241 ctggtcactt ccaccaaatt gtagtttaca gttcttattt ttatacaacc agtaaaaatc
     3301 gtaaccctgc attaattgat aggcaaattc ggtataacta attccgtttt ctccttcaat
     3361 tcttttcttt acactatctt tagccatcat ataatttatg gttatatgct tgcccgtgtc
     3421 tcttaaaaaa tcaataaatg aaatattttt aaaccaatca taattattta ccagtattgc
     3481 tgcatttggt ttttcatcat caaaagatat aaattgttta agttgtgatt gaataccttc
     3541 tatatttttt tgaagtgttt cttcgctcaa taaatttctt tcgtcacttt ttcctgttgg
     3601 gtctcctatc atacctgttg caccaccaat taaagccatt ggcttgtgcc cagatttttg
     3661 catgtgtact aaaagtatta ttggtacaag gctaccaata tgcaggcttt ctgcagtagg
     3721 atcaaaacct acataacctg tagtcatttc tttaactaat tgttcttcag tgcctggcat
     3781 tatatcagca atcattccac gccaacgtaa ttcctcaatt aaattcatac aaaatactat
     3841 ttgggcaaat gtaaaagatt agccttattg aataatgtga atatcccctt taaaagataa
     3901 ttttttaaac attttttcgt ttttaacttg ttattactgc atttaagaca atttttagcg
     3961 aaatttgtaa gcagccttta aacaacctta gctatctttt gttggatgag tgggaacgtt
     4021 ttttgttaat accgaacttt tatgaattaa tttattatga aacatttgct actaactttt
     4081 tttattgttt gttttgggaa ttcaattatt gcacaacctt tacataatga gtggattgat
     4141 tattctaaaa cttattataa gtttaaggta gattttggta tccatcctgt aacccttcaa
     4201 cccattaaaa gaaaactggt aagaataact aaagcaacat tagatagtgt tggtttaggt
     4261 aataccaatg ttgaacaatt taaattattt aggaatggtg tagaagtacc aattttcacc
     4321 tctactacaa gtggtgtttt agatagttta ggctatatag agttttgggg agaaattaat
     4381 gacggaaaac ttgataaaga attataccgt gatgccgatt accaattatc tgatatttgg
     4441 agtttacaag aagacaatgg tacttacttt ttaacaacaa gcaacgcaat aaataaaagg
     4501 ctagtaaaca ctgccaataa tgttataggc aacctacttt ctcctacttt gtattttatg
     4561 aatacagtaa tgtacacaca aaggcaaaga aggtttgaag gatatgctgc acagtcatcc
     4621 ttaccattac actcctcctc ttacgataaa ggagaaggat atgccactcg tcctattcgc
     4681 ccaattggta gtagttgtgg tcaagtatct tttactatca actttaccaa tttaaaacct
     4741 tatttaagtg gcccgccaat gaccttaaag gtgaatacta tgggtagtga aaataataca
     4801 agaactattt tagttaaaat aaatggcaat agcgtaagta attatcaaat ggattatttt
     4861 aactatgcta aagttgaaga gtctggtatt cctgtaagtt atatctcatc tggcaatgct
     4921 gctgttttac atatcaacca aagccctgta gattgtgatg aatttagatt aattaaagat
     4981 gaattaactt atccaagaac tttagatggg gacaataatg taagcttaga actaaaatta
     5041 cctgcaacag caactggcca ttatttaaaa tttttcaatt ttaattctgg agccaattca
     5101 cccattttgt acgatattac aaatggcaaa agatatatag cagataaaac tattaatgat
     5161 acattacagt ttgttactaa tccttcttta actgcttata atttggtatt agttaacaac
     5221 gatagtgcaa atattttttc tgtatcaacc cttcagcaaa gaaactttgt aaactatcaa
     5281 aatatagcca accaaggaga ttatatcatt atttcaaatc cattaatata tggcactgga
     5341 aatagtaatt atgttgaaca atataaacaa tacagaagta gtacaaaagg tggcagctat
     5401 aatgcaattg tagtagatat aaatgaaata accgatcagt ttgcatttgg tataaataaa
     5461 catccacttt ctgtaagaaa ttttttacgt tatgcaagaa atattttcac tactacacca
     5521 aaatatgttt ttataattgg taaaggttta aactatattg attttagaag taatgaagat
     5581 gaaccagata tgccttatca aaaccttgta cctacatggg ggcatcctgc atcagataac
     5641 ttacttagtg cagcagataa tttaaatcct acacccataa caccaattgg cagattaagt
     5701 gcagtatctg caaatgaggt tggagattat cttaaaaaaa ttaaacaata cgattcatta
     5761 caacaagccc cacttgtgaa tatagaagat aaaacttgga tgaaaaatgt attgcatatt
     5821 ggtggagcaa cagactacaa tattggcact tctataacca attatttaaa taaatatacc
     5881 caaataatag aggatacttt atttggtgca aaggttaaaa aatataataa attagataat
     5941 cctgctggtt atggtgctgc attagttgac tttaaaaata cttacgagtc aggtgcatct
     6001 ataataactt attttggaca ctcatcggca aatttattag attttaattt agacaatcct
     6061 aacgcttata ataaccctaa taaatatcct gtatttatag ttaatggatg cgatgcagga
     6121 aattttttga cttacgaacc acaaagacta aacataaaaa caagtatttc agaaaagttt
     6181 gttctagcac cagaaagagg tgcaattggt tatttagcaa caacaggcta tggtgcaatt
     6241 aattatttag atacatttac tacaggcttt tatagagcct taagtactat agattataat
     6301 aagccatttg gagaaagcat aaaagatgga attattaatg tattaaacac aacaggctta
     6361 aacgattttt ttgcaaggta tcatgcagaa caatttattt ttcatggaga ccctgcttta
     6421 aaacttaata cttttccttt accagatctt gttttaaaaa cagaatacac aactacctta
     6481 ccaacaaaaa ttacagttgc taaagattcg ttttatttaa aaatggatat ttataacatt
     6541 ggaaaaaaaa taaatgactc tataaattta aaaataacac gtcaatatcc taaaggcaat
     6601 agcgaagttg tacttaacaa aaaactacct gccatagcat taatgacaac tgaaacagca
     6661 gcttttccta tcattgccaa tagagataaa ggaaccacta ttttaactgc tacaatagat
     6721 gctaccaata gcattaatga aatggatgaa aataacaata ctgctgtaat ttcagtatcc
     6781 attacagatg atgaaattgt tccaatatac ccctataatt atgctatagt aaacaattct
     6841 aattttaaat tagcagcctc tactgcaaac ccacttgata ccttaaaaaa ttatattgta
     6901 gaagtagata ctacttcctt attcaattca cccattaaat acacacaaaa taaatcatcg
     6961 gttggtggtg ttgttgaatt tgattatgga ttaaccttac aaaacaatac aacttatttt
     7021 tggagagtag caacacaagg aagtagttat tggaacactg tttcgtttac ttataaaagc
     7081 agcccagata ttggctttga acaaagacac tttcttcaac atacagatag taaactaaca
     7141 gatattattt tagatagtag ctccagaaaa ttcaaatttg ggcaaacttt aaataacctc
     7201 tttgttgttc attctattta tccttatagt ggaacggaag atcaacattt tagtataaaa
     7261 gtaaatggta caggtattat tgcaagtgct tgtttaggta attctgtaat tataaatgtt
     7321 tttgatactt taagttttaa cccatggata aatactacca atccgtttgg tgctacacca
     7381 acttgcctag aaacaagaaa atataatttt gaatattctt atttagatgt atcaggtaga
     7441 aataatgcca aaagcttttt agaatctata ccaaatggca tgttagtagc tgttcgtatg
     7501 gtgtatgatg gagcctctgt ttgggcaaat gattgggcca atgatacatt attgaatggc
     7561 tcaggtaatt ccttgtatca tttcttaaaa aatcaaggct ttgcaataga ttctttcaat
     7621 catccaagaa cctttgcttt tatttttaag aaaaacgact cgttacattt tactccaaaa
     7681 tatttattta gtgaaggatt atatgatagg gttattttaa atgcagatta taatagtgat
     7741 gacactttag gctatgttgt ttctccaaaa tttggacctg caaaagcatg gaaatttgca
     7801 aaatggaatg gcaatggcaa tggtagcagt tttgctaaaa tgaatattat tggaattgat
     7861 actaacggta ttgaaaatgt attatttaat aatatggata ctttgcaaac aagttttgat
     7921 ataagtagta ttaatgcaca acaatatcct ttcgtgaaat tatctatatt aaatcaagac
     7981 tctgtaaaag catttcctta tcagcttagt aattggagtg ttgaatatgt tggtgtgcca
     8041 gaaggtgcag ttgctcctaa tttacatatc aatattcctg atacaattgg tttaacatca
     8101 agctatgcaa gcgatacttt aaaaggcagc tttgctttta aaaatgtaag taaagaaaat
     8161 tttgatagtt taacagtaaa agtagtttta actaatttaa gattaggaac tgttcataca
     8221 tttaaccttg ctaaaacaaa accattactt gcaggggata cattgcatat taattttgct
     8281 atagatgcct cgttaatgtc aagagatgaa tataacttgt atgtagcagt taatgaaaat
     8341 gcagctcaac cagaacaata tttatttaat aactttttgt ataagtatat ttattttaaa
     8401 acaagtgtaa ttgttcctgt aaaattatta aatctcactg caaaaattaa tggtacagga
     8461 acagatataa cctggcaagt tgcagaggaa gttaatgttt taaaatatga agttgaacat
     8521 agtaataatg caattgattt taaaactata ggtagtgtag atgccaatgg taaaaacaat
     8581 tatttgttac atcatgcaca acctacctat ggtaaaaatt attacaggct aaaaatttta
     8641 aatacagatg gtagctataa ctattctacc atacgacttg taaattttgg aaaaggagtt
     8701 atagtaaatg tgtatcctaa ccctgtaata ggcaaattaa atattgcagt tagtaataat
     8761 aataacaagc aagtacaagt aaacttatat aacaattacg ggcagttatt atttacagaa
     8821 gaatttaaaa ataattcaac tattgatatg agtaaattat catcaggtgt atatatttta
     8881 caagtaaatg atggtattca atggcaaact tttagaatag taaaataatt agattcatat
     8941 aataaaaaat ggtgtacaac aatatttgta caccattttt ttataaaaat aattagtaga
     9001 attagataat taataataca tctccatagc tataaaaacg atatttatct tttatagctt
     9061 tatgataagc ttccatagtt aaatcataac ctgcaaatgc acaagtcata ataaataaac
     9121 ttgttttagg taaatgaaaa ttagttacca tactatctgc aatactaaaa tcataaggag
     9181 ggtgaataaa atggtttgac caaccttcag aagattttaa tcttttttga gcagtaaaat
     9241 tagtttctaa tgccctcata gtagttgcac caattgcaca aacacgatgg ccgttatctt
     9301 tggctttatt tacaatttga actgcacctt ctggtatatt gtaatactca gcatccattt
     9361 tatatttact aaggtccttc
//
LOCUS       DAOQPK010000074         9279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_3984_length_9279_cov_3.968777, whole genome shotgun sequence.
ACCESSION   DAOQPK010000074 DAOQPK010000000
VERSION     DAOQPK010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9279
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3984_length_9279_cov_3.968777"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="PLU36_05025"
     CDS             complement(<1..168)
                     /locus_tag="PLU36_05025"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HMZ46145.1"
                     /translation="MIDIAPGILLISDPFLKDPNFARSVVLICDHQQEGSCGFVLNKL
                     YDRSLKDMLTDF"
     gene            343..1677
                     /locus_tag="PLU36_05030"
     CDS             343..1677
                     /locus_tag="PLU36_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMZ46146.1"
                     /translation="MKKTLPLIVTLITLSLLGLIILQASWLKNLIEVRQAHVFNKIQT
                     VTTQVATDLSYAAFAGPVIRLPRKSNKISAEFHLRLLKPPSIGDKFTPQEIYAKLRKA
                     FDDVDLKKMKFEFAIANSDGDYEMQSLNYAKEFYDTTSNKRIHNYLVPETGTEIDQVV
                     QAEQMIVIVPDFTKQVWRSLQWIIIGAIIFMLIIIAAFYVTLKSLFNQKKLSEIKSDF
                     INNMTHEFKTPLATISLAVDAIKNEKVKNDSDRFQYFSNIIKEENRRMNKHVETILQA
                     ALMEKQELKLNINRIALHPLLNNVIEAYNLQLLEKNGFIETNLNAKNDVVEADEVHLT
                     NLLSNLIDNAIKYSKDNLVIKISTSCNNKIFSIKIEDNGIGMNKESVKRIFEKFYRAH
                     TGNIHNVKGFGLGMSYVKTVIDAHKGRIKVESTLGKGSTFWVELPRTYTSKK"
     gene            complement(1696..2034)
                     /locus_tag="PLU36_05035"
     CDS             complement(1696..2034)
                     /locus_tag="PLU36_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HMZ46147.1"
                     /translation="MQGSLPVSFTIDAINEIKRLISEEDFNASKKLRVGVKGGGCSGM
                     TYVLGFDEPTAEDTEYTTNDFSFIMNKSHSLYLIGMQIDWSDGLNSRGFTFTNPNVSK
                     TCGCGTSFAV"
     gene            2198..2710
                     /locus_tag="PLU36_05040"
     CDS             2198..2710
                     /locus_tag="PLU36_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46148.1"
                     /translation="MDSPIVIAFIIAIAVFFIGTLVVPYFRDKRKQKAKNSTNSITSK
                     QLQLQAYERLILLTDRIALPNLVPRLSQPGFTAKEMQLFIINNIIQEFEYNVTQQMYV
                     SNDAWTAVKNLKEQNIGITNQIGQLLPETATGLDLCRVLTEFLLKDKRGTLHELVSEA
                     ISFEAKKIMK"
     gene            complement(2713..3453)
                     /gene="aroE"
                     /locus_tag="PLU36_05045"
     CDS             complement(2713..3453)
                     /gene="aroE"
                     /locus_tag="PLU36_05045"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611044.1"
                     /note="AroE; catalyzes the conversion of shikimate to
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HMZ46149.1"
                     /translation="MKIFGLVGNSLSHSMSKKYFEDKFLKENLPDYYFKNFELTDINQ
                     LKELLATTKNLCGLAVTIPYKQAVLPFLNFATKEVETISACNCIKITDNVLYGFNTDI
                     IGFEKSFTSLLQPQHQKALILGNGGAAKAVKYVLQKLAIPYLIVSRNKEKQYPSISYQ
                     EINEQIVEEYKIIINTTPLGTFPAINNCANIPYNLLNKTNYLYDLVYNPAVTKFLELG
                     KQQGALIKNGSDMLIIQAEENWKIWNQN"
     gene            complement(3453..4229)
                     /locus_tag="PLU36_05050"
     CDS             complement(3453..4229)
                     /locus_tag="PLU36_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HMZ46150.1"
                     /translation="MNFNLTQMPDRTSKPRTNGLTMVMDKGLGLTDAKNFLEIATPHV
                     DLVKLGFGTSHVTPNLRKKIETYKAAGIHVYFGGTLFEAFLIRNQIDDYLAICKDYEI
                     DYIEVSDGSITIPHTEKCGYIEKMTKYATVLSEVGSKDATHIIPPYKWIELMRAELEA
                     GSSYVIAEAREAGNVGIYRGTGEVREGLVQEILTQIPSEKIIWEAPQKAQQLYFLELI
                     GCNVNLGNIAPTEILALEAMRVGLRGDTFRLYLDKEKAIY"
     gene            complement(4340..5761)
                     /locus_tag="PLU36_05055"
     CDS             complement(4340..5761)
                     /locus_tag="PLU36_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ46151.1"
                     /translation="MKDQNHSHEDREAMNALLMHYHNLKNGRNHPFIEEEDFERIIDY
                     FDDNNNINEALEAAEISISQFPYSSTLLLRKADLLIATKKYIEALEILNQAALYDSTD
                     IGLYILRTDALLALDKQEEAVEVLAEALQLFEGNEKLDLLFELADVYDDFEEFDKVYD
                     CLKLILEEDPNNEEALYKICFWTDFTGRNEESINLYKKIIDDFPYNEIAWFNLAAAYQ
                     GLKLYEKAIDAYQFAIVIDEKFDYAYRNMGDAYLRLKKYREAIEVLEKVLELTRPEDV
                     IYEAIGHSYHRMGNTGQARFHYKKAVHLNPDDSKLYYKIATTYMAENQWQQAIKQLEQ
                     ALQIHKNVPEYNLAMGECKMKLNLFKDAVVYFSIVVNNKPKKIAGWEALIRCLFKATF
                     YDEAIEQCKAALKITENKPIFIFYYSTALLKVGKTKEGLLQLEKAMEKAPKLLKKFIE
                     LNPAILQNNDVVDIVAKYKKYNK"
     gene            5892..6236
                     /locus_tag="PLU36_05060"
     CDS             5892..6236
                     /locus_tag="PLU36_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46152.1"
                     /translation="MFRIKNIQKISVAVVLLLAVQVLYATNIFSGGIERKNEVSKYSL
                     KNLSRYNNKTLTLSTAKYSYKLKSTLLNNNSTLNNSRSSILLSNGNTTFIYPYKSKVK
                     VPKFKTPSPTKF"
     gene            6416..7636
                     /locus_tag="PLU36_05065"
     CDS             6416..7636
                     /locus_tag="PLU36_05065"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HMZ46153.1"
                     /translation="MQIPFSPPFINENIELEVLDALRSGWITSGPKVKALEQEVCNYT
                     NAEACLCVNSATSAMMLVLHWYGVKKGDEVIVPAYTYCATALAVMHLGATPIMVDVED
                     DFNISVSKIKLAISSRTKAIMPVDFAGWPCNYSEINKLINEPEIKQKFKAETEQQKKL
                     QRILVLSDAAHSFGAVYNNKKLGTAADITIFSFHAVKNLTTAEGGAICLNLPLPFNNN
                     DVYATLRLWSLNGQTKDAFSKSKAGSWRYDIVYPGFKMNLPDVLAAIGLAQIKEYNEV
                     LLKERKRVFDFYSKTFSNYSWAMLPPYDVPNASSSYHLYPLRIKDINEEQRDRIIEKI
                     AETGVAVNVHFQPLPLLTVFKQMGYDINNFPSAYNNYKAEISLPIYPQLNNNKCTYIV
                     DNVVNAIKEVLING"
     gene            7629..8228
                     /locus_tag="PLU36_05070"
     CDS             7629..8228
                     /locus_tag="PLU36_05070"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HMZ46154.1"
                     /translation="MDSIRLLDILFSIIGIIILLPLFILIALCIKLTSKGSIIFKQVR
                     VGKNNTDFKLLKFRTMVAFAEAKGQLTVGGKDNRITAIGYYLRKFKLDELPQLINVLM
                     GQMSLVGPRPEVRKYVALYSEQQIEILKVLPGITDFASIEFRNENELLANAIHPEEFY
                     IKEIMPKKIELNKKFIKDRTIYNYFLILFKTVFTAIKGK"
     gene            8444..>9279
                     /locus_tag="PLU36_05075"
     CDS             8444..>9279
                     /locus_tag="PLU36_05075"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46155.1"
                     /translation="MKHLRKFLLKNRIAPKWAVFGLDMLIACFAIVYANYLRFNFDFQ
                     FITLNDLIDDLVIVIVTNSILFYIFKTFHGIIRFSGFQEAFRSIAAIFYSFFIMIVMN
                     VILSSLQLPQFTPISVLFIYFFIASFLLFGYRLLVKHLYSISINNEEDTHVVIYGAGQ
                     NGSLLKKTIEQTSNNRYKIVAFIDDEEDLIGKTIDTVKIYPVSHLQMTFKNWQIKTLF
                     FAKQDFDLDEKNKIVDYCLESNVKVMNIPPMKEWIQGHLNVNQLKEVKIEELLGRKQI
                     SL"
BASE COUNT         3282 a         1327 c         1314 g         3356 t
ORIGIN      
        1 aaagtctgta agcatatctt ttaacgaacg atcgtacagc ttatttaaaa caaagccaca
       61 acttccttct tgttgatgat cgcaaattaa aacaacactt cttgcaaaat taggatcttt
      121 caaaaatggg tcagaaatta ataaaatacc tggtgctata tctatcataa ccgaaaatta
      181 aatcattcaa gcaaaattta aaaatttaat attatcagta agttaaaatt caattaaata
      241 aaggttgcat ttttacttaa attgctttgc aatactatat ttgttaatac tgttttataa
      301 aacttaatta gtttaaaagt gtaattactc tacaacatta gcatgaaaaa aactttacct
      361 ttaattgtaa cgcttattac actttcatta ctagggctta ttattttaca agcatcttgg
      421 ttaaaaaatt taattgaagt aaggcaggct catgttttta ataaaataca aactgtaact
      481 acacaagttg ctacagattt aagttatgct gcttttgctg gcccagttat aagattacca
      541 agaaagtcga ataaaatttc tgcagaattt catttaaggt tattaaagcc accaagcata
      601 ggcgataaat ttacaccaca agaaatttat gctaagctaa gaaaagcttt tgatgatgta
      661 gatttaaaaa aaatgaaatt tgagtttgct attgccaata gcgatggtga ttatgaaatg
      721 caatctttaa actatgctaa agagttttat gataccactt caaacaaaag aatacataat
      781 tacttagtac ctgaaacagg aactgaaatt gaccaggtgg tacaggccga acaaatgatt
      841 gtaattgtac ctgattttac aaagcaggtt tggagaagtt tgcaatggat aattattggt
      901 gctataattt ttatgcttat aattattgca gcattttatg ttacacttaa aagtttattc
      961 aatcagaaaa agttaagcga aataaagagt gattttatta acaacatgac ccatgaattt
     1021 aaaacaccat tagccaccat ttctttagca gtagatgcta ttaaaaatga aaaagtaaaa
     1081 aatgatagcg ataggtttca atactttagt aatataatta aagaagaaaa tagaagaatg
     1141 aataagcatg tagaaaccat actacaagca gctttaatgg aaaaacaaga attaaaacta
     1201 aatattaatc gtattgcttt acatccatta cttaataatg taattgaagc ttataattta
     1261 caactattgg aaaaaaatgg ctttattgaa acaaacttaa atgcaaaaaa cgatgttgta
     1321 gaagcagatg aagtacattt aactaatttg ttatctaatt taatagataa tgcaataaaa
     1381 tattctaaag ataatttggt tataaaaata tcaacctctt gtaacaacaa aattttttcc
     1441 attaaaatag aagataatgg tattggtatg aacaaagaat cggttaaaag aatttttgaa
     1501 aaattttata gagctcatac aggcaatatt cataatgtga aaggttttgg tttaggaatg
     1561 agctatgtaa aaactgttat agatgcacac aaaggaagaa ttaaagtaga aagtacttta
     1621 ggaaaaggaa gcactttttg ggtggagtta ccaagaactt atacttcaaa aaagtaactg
     1681 atttttatta tttctttaca ctgcaaatga agtaccacaa ccacaggttt tacttacatt
     1741 ggggttagta aaagtaaacc ctctgctatt taaaccatca ctccaatcta tttgcattcc
     1801 tattaaatac aaactatggc ttttgttcat aataaaagaa aaatcgttgg ttgtgtattc
     1861 cgtatcttct gcagtaggtt catcaaatcc taaaacataa gtcataccac tacagcctcc
     1921 tccttttact ccaactctca attttttaga agcattaaaa tcctcttcac taattaatct
     1981 ttttatttca ttaatggcat ctatggtaaa actaactggc aatgaacctt gcataacttg
     2041 taatattttt acaaaagtaa aaagtaaata ataaactagt ataaaaagct attacgataa
     2101 ttggaataag aaatgttatt ttttaaggtt ttgaataaac tatactaaat tcaatttttt
     2161 atggtttgct aaaaactata tttgcaaaaa tttatatatg gatagcccta ttgtaattgc
     2221 ctttattatt gccatagctg ttttttttat tggtacactt gtagtgcctt attttagaga
     2281 taaaagaaaa caaaaagcaa aaaattctac caatagtatt acctcaaagc aactgcaatt
     2341 acaagcatat gaaaggctaa ttttattaac cgatagaatt gcattaccta atttagtacc
     2401 aagattatcg cagcctggat ttactgcaaa agaaatgcag ttatttatta ttaataatat
     2461 tattcaagaa tttgaatata atgttaccca acaaatgtat gttagcaatg atgcttggac
     2521 tgctgtaaag aatttaaaag aacaaaatat tggtattact aaccaaatag gacaattatt
     2581 accagaaact gctacaggat tagatttgtg tagagtatta accgagtttt tactgaaaga
     2641 taaaagagga actttacatg aattagtgag tgaagcaata agttttgaag ccaaaaaaat
     2701 tatgaagtaa gtttaatttt ggttccaaat tttccaattt tcttctgctt gaatgattag
     2761 catatcgcta ccatttttta ttaacgcacc ttgctgtttt cctaattcta aaaattttgt
     2821 aacagcaggg ttgtacacta aatcatataa ataatttgtt ttgttaagta ggttatacgg
     2881 aatatttgca caattattta tagcaggaaa agtacctaat ggagtagtat taataataat
     2941 tttatactct tcaactattt gttcattaat ttcttgataa gaaatggaag gatattgttt
     3001 ttctttattt ctagaaacaa ttaaataagg aattgctaat ttttgaagta cgtattttac
     3061 tgcttttgct gctcctccat tgccaagtat taaggctttt tgatgctgag gttgaagcag
     3121 tgatgtaaaa gatttttcga aaccgattat gtctgtatta aaaccatata atacattatc
     3181 tgtaattttt atacaattac aagcactaat tgtttctacc tcttttgtag caaaatttaa
     3241 gaaaggtaaa acagcttgtt tataaggtat tgtaactgct aacccacata agtttttagt
     3301 agtagcaagc aattctttta attgattaat atcagtaagc tcaaaatttt taaaataata
     3361 atcgggtaaa ttctctttta gaaatttatc ttcaaagtat ttttttgaca tagagtgaga
     3421 aagagaattt ccaactaagc caaatatttt cattaataaa ttgctttttc tttatctaaa
     3481 tataatctaa aagtatcgcc acgtaaacca actctcatag cttctaatgc taatatttct
     3541 gttggtgcaa tatttcctaa attaacatta caaccaatta gttctaaaaa gtatagttgc
     3601 tgtgcttttt gtggagcttc ccaaataatt ttctcacttg gtatttgagt taatatttct
     3661 tgtactaatc cttctcttac ttctcctgta cctctataaa tacctacatt acctgcttct
     3721 cttgcttcgg caattacata actgctacca gcttctaatt ctgctctcat taattcaatc
     3781 catttatatg gcggaataat atgtgttgca tctttgctac ctacttcact aagtacggta
     3841 gcatatttag tcattttttc tatataacca catttttctg tatggggtat tgtaatggag
     3901 ccatcactaa cttcaatata atctatttca taatctttac aaattgctaa ataatcatct
     3961 atctggtttc gtattaaaaa tgcttcaaac aaagtaccgc caaaataaac atgaatacca
     4021 gcagccttat aggtttctat tttttttctt aggttaggag taacgtgtga ggttccaaaa
     4081 cctagtttaa ctaaatctac atgaggagtt gctatctcta aaaaattttt tgcatctgtt
     4141 aaccctaatc ctttatccat aaccattgta agtccattag ttcttggctt acttgtacga
     4201 tcaggcattt gtgttaaatt aaaattcatt agtttttatt ttttataact tttgcaaaag
     4261 taagcttatg cgtaaaacta atttgtataa attataggaa taaaatggat gagtgctaaa
     4321 ttggtatttt ttaaaaaaat tatttattgt actttttata ttttgcaaca atatcaacta
     4381 catcattatt ttgaagaatt gctggattta attctataaa ctttttcaat aatttaggag
     4441 ccttttccat tgctttttct aattgtaata aaccttcttt agttttacca acttttaaaa
     4501 gtgctgtgct gtaataaaaa ataaatatgg gtttgttttc ggtaattttt aatgcagctt
     4561 tgcattgttc tattgcttca tcataaaatg ttgctttaaa taagcagcgt attagagctt
     4621 cccatcctgc tatttttttt ggtttgttat ttactacaat gctaaagtaa accactgcat
     4681 ctttaaacaa gtttaacttc attttacact ctcccatagc taagttatac tctggcacat
     4741 ttttatgtat ttgcaatgct tgctcaagct gcttaatagc ttgttgccat tggttttctg
     4801 ccatgtaagt ggtagctatt ttatagtata atttactatc gtcggggttt aagtgtactg
     4861 cttttttgta atgaaacctt gcctggccag tattacccat tctatgatac gaatggccaa
     4921 ttgcttcata aataacatct tctggtctgg ttaattctaa aactttttct aatacctcaa
     4981 tggcttctct atattttttt aatcgcaaat atgcatctcc catatttcta tatgcataat
     5041 caaacttttc atcaattact attgcaaact gataagcatc tatggctttt tcatacaact
     5101 ttaaaccttg ataagctgct gcaaggttaa accaagcaat ttcgttgtag ggaaaatcgt
     5161 caataatttt tttgtataaa ttaatgcttt cttcatttct tcctgtaaaa tctgtccaga
     5221 aacaaatttt atataaagcc tcttcgttat tagggtcttc ctctaaaata agttttaagc
     5281 aatcatatac tttatcaaat tcttcaaaat catcatatac atctgcaagt tcaaacaaca
     5341 aatctaactt ttcattgccc tcaaaaagtt gtaaagcttc tgctaaaact tcaactgctt
     5401 cttcttgttt atctaaagct aaaagagcat ctgttcttaa aatgtataat ccaatatctg
     5461 tgctatcgta aagggcagcc tgatttaaaa tttctaaggc ttcaatatat tttttggttg
     5521 ctattaataa atcggccttt cttaataaaa gagtagagga gtaagggaat tggctaatac
     5581 ttatttctgc tgcttctaat gcttcgttaa tattattatt gtcatcaaag taatctataa
     5641 tacgttcaaa atcttcttcc tctataaaag gatgatttct tccattctta aggttatgat
     5701 aatgcattaa taatgcattc attgcctccc tgtcttcgtg tgaatgattt tgatctttca
     5761 ttataaaaag aataactaaa ttaaatatag gaaaaaaaat ttaatctaat atgtaacttt
     5821 ttatactttt caacgtcttg ttaataacat aaaatttgtt aaatattttt aaaataacta
     5881 tttttgtaaa aatgtttaga attaagaaca tacaaaagat atcagttgct gtggttttac
     5941 tacttgcagt acaagtattg tatgcaacta acattttttc tggcggcatt gagcgtaaga
     6001 atgaagtaag taaatattct ctaaaaaacc ttagccgcta taacaataaa actttaactt
     6061 taagtactgc aaagtatagt tataaattaa aatctacttt actaaataac aattctactt
     6121 taaataattc tcgttcatct attttgcttt ctaatggcaa tactactttt atatatcctt
     6181 ataaatctaa agtaaaagtg cctaaattta aaacaccttc tcctacaaag ttttaattaa
     6241 ttaacttctt tctttttttc ttaattcgtg tattattatt tctgcatata aaataatatt
     6301 ttattttttt gcgtttataa acccgttaca tcaaaatatt tatccctaaa ttttattcct
     6361 tacttttcat ttcctttaat ttcgggtacg tttaaaaaat tgattatata tatttatgca
     6421 aataccattt tctccgcctt ttatcaatga aaatattgag ttagaagttt tagatgcttt
     6481 aagaagtggt tggataactt caggaccaaa agttaaagcc ttagagcaag aggtttgtaa
     6541 ttatactaat gctgaagcat gtttgtgtgt aaactctgcc acatcggcaa tgatgcttgt
     6601 tttgcattgg tatggcgtta aaaaaggcga tgaagtaatt gtgcctgcat atacttattg
     6661 tgctactgct ttagctgtga tgcacttggg tgcaacacca ataatggtag atgttgaaga
     6721 tgattttaat atctcagttt caaaaattaa attggcaatt agtagcagaa caaaagcaat
     6781 aatgcctgtt gattttgccg gatggccttg caattattct gaaataaata aattaattaa
     6841 tgagccagaa ataaagcaaa aatttaaagc cgaaactgag caacaaaaaa agttacaaag
     6901 aattttagtg ttaagcgatg ctgcacactc ttttggtgct gtgtataata ataaaaaatt
     6961 aggaactgct gctgatatta ctattttttc ttttcatgca gtaaaaaact taactactgc
     7021 cgagggtgga gctatttgtt taaaccttcc tttaccattt aataataatg atgtttatgc
     7081 aaccttaagg ctttggagtt taaacggaca aacaaaagat gcattctcta agtctaaagc
     7141 tggtagctgg agatatgata ttgtttaccc tggctttaag atgaatttgc cagatgtttt
     7201 ggctgctatt ggcttagctc aaattaaaga atataacgaa gtattattga aggaaagaaa
     7261 aagagttttt gatttttatt ccaaaacatt ttctaactat tcatgggcaa tgttaccacc
     7321 ttatgatgta cctaatgctt cttctagtta tcatttatac cccttaagaa taaaagatat
     7381 aaatgaagag caaagagata gaattattga aaaaattgcc gaaacaggcg ttgctgtaaa
     7441 tgtccatttt caaccattac ctttattaac tgtttttaag caaatgggct acgatattaa
     7501 taattttcct tcggcatata ataactacaa agcagaaatc tctttaccaa tttatcctca
     7561 gttaaacaat aataaatgca cctacatcgt tgataatgtg gtgaatgcaa taaaagaagt
     7621 tttaataaat ggatagtatc agactattgg atatactttt ttctatcata ggaataataa
     7681 tattgctacc actattcatt ctaattgctc tttgtattaa acttacttct aaaggaagta
     7741 ttatttttaa acaagttaga gttgggaaaa ataataccga ttttaaatta cttaaattta
     7801 gaacgatggt agcttttgca gaagcaaaag gacaacttac agttggtgga aaagataata
     7861 gaattaccgc aattggttac tatttacgaa aatttaaact agacgaatta ccacaattaa
     7921 taaatgtatt aatggggcaa atgagtttgg tgggaccaag accagaagtt agaaaatatg
     7981 tggcccttta ttctgagcag caaattgaaa tattaaaagt attaccaggt attacagatt
     8041 tcgcctccat tgaatttaga aatgaaaacg aattattggc aaatgctatc catccggaag
     8101 agttttatat taaggaaata atgcctaaaa aaatagaact taataaaaaa tttattaaag
     8161 atagaacaat ctataattat tttttaatac tttttaaaac tgtatttaca gctataaaag
     8221 gcaaataata aataacaaca taatacatag agtttgtact atggtagatt gcatgttaag
     8281 taagattagt ttacgtttta aaaaacaaga gtgttataaa attttaaact atatttgtat
     8341 tggcttaaat ccatcttatt ttacttgcct tctaagaaaa acactttagt acatccaata
     8401 tttataaaaa aaaatcgtta ttaaagcaaa acgtacttaa taagtgaaac atttgaggaa
     8461 atttttatta aaaaacagaa ttgcacccaa gtgggcagtt tttggcttag atatgcttat
     8521 tgcctgtttt gccattgtat atgccaacta tttacgtttt aatttcgatt ttcagtttat
     8581 tactttaaat gatttaatag atgatttagt tattgttatt gtaactaata gcatattgtt
     8641 ttatatcttt aaaacctttc atggtattat aagattctct ggttttcaag aggcatttag
     8701 aagtattgca gctatttttt attctttctt tatcatgatt gtaatgaatg taattttaag
     8761 ttcattacaa ttgccacagt ttacacccat ttcagtttta tttatttact tttttattgc
     8821 ttccttttta ttatttggat ataggttatt ggtaaaacat ttatatagta taagtattaa
     8881 taacgaagag gacacacatg tagttattta cggtgctggg caaaatggat cattattaaa
     8941 gaaaacaata gaacaaactt caaataacag gtataaaatt gtagcattta tagatgacga
     9001 agaagatttg ataggtaaaa caattgatac tgtaaaaatt tatcctgtta gtcatttaca
     9061 aatgacgttt aagaattggc aaattaaaac tttatttttt gccaagcaag attttgactt
     9121 agacgaaaaa aataaaatag tagattattg tttagagagc aatgtgaaag taatgaacat
     9181 tccacctatg aaggagtgga ttcaagggca tttaaacgtt aatcaactta aagaagtaaa
     9241 aattgaagaa cttttaggcc gcaagcaaat tagcttaag
//
LOCUS       DAOQPK010000075         9223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_4025_length_9223_cov_3.856894, whole genome shotgun sequence.
ACCESSION   DAOQPK010000075 DAOQPK010000000
VERSION     DAOQPK010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9223
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4025_length_9223_cov_3.856894"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /gene="queG"
                     /locus_tag="PLU36_05080"
     CDS             <1..585
                     /gene="queG"
                     /locus_tag="PLU36_05080"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HMZ46156.1"
                     /translation="IITEKIGQVNGRGFVDSAPVLEKTWANKSGLGWVGKNGILITKH
                     SGSFFFIATLIVDIELAYDNPFTKDYCGSCTKCIDNCPTEAILPQKVINASKCISYFT
                     IELKDELIDESMKGKFKNWMFGCDICQDVCPWNRFSKPTNEIAFTPIPEVLNLTTQQW
                     EELSEESFKKIFKNSAIKRSKYKGILRNLKFLKQ"
     gene            complement(587..1930)
                     /locus_tag="PLU36_05085"
     CDS             complement(587..1930)
                     /locus_tag="PLU36_05085"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMZ46157.1"
                     /translation="MQKKNITIAGAGLVGSLLGIYLAKRGYSVSIYERRSDMRKESLS
                     AGRSINLALSDRGLLALKKVGLEDEIKKISIPMHGRYIHNADGSTAFQPYGKEGQFIN
                     SVSRATLNIKLMELAEQEGVKIYFNHKCANINWNKNEINFDTENGNSITTNFDLLFGA
                     DGAYAATRLQHQVHHSKFDYQQYYIDCGYKELTIPPTKEGDFAMEVNALHIWPRKDYM
                     LIGLPNLDKTFTCTLFFPFEGETSFSKLDTKEKVKSFFEKTFPDAIKLMPNYLDDFFE
                     NPTSSLVTVKCYPWIRDDKFTLIGDAAHAVVPFFGQGMNCGFEDCRILDELMEANENN
                     WHNILEAFQQTRKPDADAIADLAIENFIEMRAKTADPKFLLQKKIEVRLHEKYPNQWI
                     PAYAQVTFSPNIRYSDALKRGQIQEKIMQQIMQIPNIEAIWDSNEVEEKILSLIP"
     gene            complement(2000..5464)
                     /locus_tag="PLU36_05090"
     CDS             complement(2000..5464)
                     /locus_tag="PLU36_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46158.1"
                     /translation="MKYLLNILAFGFALLLFNSANAQIITTSNKVVKKIASKSMFAFD
                     GRKIVPHTVIIYNYDSSFYSIDYTKNIINWLKPTASDSVLIEYRYFNFKINENAYRYN
                     YDSVINNFIAAPQVISQSKQNENVGLFGQTISINYNGSFGRNLSFGNNQDAVFNSQLN
                     LQISGMLGDSIEIAAAITDNNIPIQPDGTTQQLNEFDKILLQFKKRNMQLDLGDIDVR
                     SNQYYFLNFYKRLQGASYKSLNSKKNSNFSFAGAIAKGKFARNIFNGLEGNQGPYKLA
                     GNNNEFFLIILAGTERVFMDGEMLQRGEDQDYIINYNTAEIVFTPKHMITKDKRIQVE
                     FEYADRNYLNYLVYAAKEMKVNKNLQINIAAYSNNDAKNSPINQQLNANQKQFLNSLG
                     DSIEQAFYHSVVLDSFSNTKILYAKRVNPINALWDSIYVYSTHADSAKYYLNFIEVGI
                     NKGNYIPLFNSANGRVFQYIAPINGVKQGNFEPAIFLVTPKKQQIFTANAIYNINRKT
                     TLITDVALSNYDVNTFSSKNKNDNIGFAAKFLLQNNNTFHNKYQLQTKIGYEWVNEKF
                     KPIERLRTVEFARDWGLPLITSLATEHLPKVSLELKNLNNNNKIQLTTNGYLRSDKYY
                     AIKNQVSLYQYQPKNAISIKLNSSLVNIFTPNSKGYFVKINSEVSRVLKQINNYELGF
                     NYSLERNLQSNKFTTSLEPISFSFENIGAFIKSNERKNNQWAFHFFTRKNELPFQSQL
                     LQTDRSNNYNFSLHLLNNHNHQLKFNATYRQLFVQNNFLTNLQPDKTLLSRTEYKVNE
                     WNGFLTGFVMYELGTGQEQKRDLTYIEVPAGRGEYTWNDYNLDGIPQLNEFEIALYPD
                     LAKYIRIFTPTNQFVKASYLQFNYNLQITPKAIMAKFRNEKFKKFISKFMFQSSLQTS
                     QKVIADNKIQFNPFKKNITDTTLIHIQQQLSNTISFNRYSNIWGIDVSNFQNKSKALL
                     TYGFESRWMNEWLVKTRINFKKVYTLELLNKFSTTSLITPSFNNRNYNIKSFSVEPKL
                     TYTYLTRYRLQLSYIFVQKENNIIYGGEKAINNIIQAEAKLNIFNNTSLLTKFNMQHI
                     NFNGNIGTTTSYIMLDALLPGKNYLWMVELTKRLGNNLEFNINYEGRKPASSKMIHIG
                     RAGIRALL"
     gene            5722..6933
                     /gene="sucC"
                     /locus_tag="PLU36_05095"
     CDS             5722..6933
                     /gene="sucC"
                     /locus_tag="PLU36_05095"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HMZ46159.1"
                     /translation="MNLHEYQAKELLKKYNVPVQEGIAVESVMAAEDAYRQIKTQTNN
                     NFAVIKAQIHAGGRGKGKVNESGLNGVKVAKSLEDISTYAKGILGGTLVTIQTGPAGK
                     KVNKVLIAQDVYYPGPNPVKEFYLSILLDRAKGQNVIMYSTEGGMDIEEVAHNTPEKI
                     FKEWVHPSGGLQGFQARKIAFNLGLSGDAFKSCVKFVTNLYNAYVGLDCSMLEINPLF
                     KTSDDKIIAVDCKMGLDENALMRHPDLAALRDITEEDPTEVEAGQFNLNYVKLEGNVG
                     CMVNGAGLAMATMDMIKLSGGEPANFLDVGGTANAQTVEAGFKIILKDPNVKAILINI
                     FGGIVRCDRVAAGVIEAYNKLGNINIPIIVRLQGTNAAEAKKLIDESGLKVQSAILLS
                     EAADLVKKALA"
     gene            complement(7088..>9223)
                     /gene="fusA"
                     /locus_tag="PLU36_05100"
     CDS             complement(7088..>9223)
                     /gene="fusA"
                     /locus_tag="PLU36_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HMZ46160.1"
                     /translation="QLRNIGIIAHVDAGKTTTTERILRYTGMIHKIGEVHDGGATTDW
                     MEQEKERGITITSAAVSCQWNFPTILGKATPETKKYYFNIIDTPGHVDFTVEVERSMR
                     VLDGLIALFSAVDGVEPQSETVWRQANRYKVPRIGFVNKMDRAGADFLNVVKQVKEML
                     GAKSVPLQLPIGAEDNFKGVVDLIKMKGIVWHAETEGMTFDEIPVPEDMMEDVKFWRQ
                     NLIEAVAEYDDKLMEKFFENPETITEEEVHEAIRKACIDLSIVPMMCGSSFKNKGVQT
                     ALDAVCRYLPSPVDIEETIGTNPDTGEQEVRKADAKEPFSALAFKIMTDPFVGRLAFF
                     RCYSGHLDAGSYVLNVRSGKKERISRIMKMFANKQNPIDFIEAGDIAAAVGFKEIKTG
                     DTLCDEKAPITLENMFIPEPVIAIAIEPKTQADVDKMGMAIAKLIEEDPTLRVNTDED
                     TGQTILRGMGELHLEIIIDRLRREFKVEVNQGAPQVAYKEAFHSTIEHREVLKKQSGG
                     RGKFADIQFEIGPADEEWIKENEGKSFQFVNDLFGGSIPKEFVPAIIKGFETSMVTGV
                     LAGYPVNNMKVRVFDGSYHDVDSDSMSFELCAKSGFREAGRKAKPVLLEPIMKVEVLT
                     PDQYMGDVTGDLNRRRGMLEGMDSRNNLQVIKAKVPLSEMFGYVTQLRSLSSGRATST
                     MEFSHYTPAPNNIAEEVIAKNKGKVKVEE"
BASE COUNT         3068 a         1509 c         1347 g         3299 t
ORIGIN      
        1 attataaccg aaaaaattgg tcaagtaaat ggccgtggtt ttgtagatag tgcacctgtt
       61 ttagaaaaaa cctgggctaa taaaagtggc ttgggttggg taggaaaaaa tggaatatta
      121 ataacaaaac attctggctc cttttttttt atagcaacat taattgtaga tattgaattg
      181 gcttatgata acccttttac taaagactat tgtggctcat gcacaaaatg tattgataac
      241 tgccccaccg aagcaatttt accacaaaaa gtaattaatg caagtaagtg tattagttat
      301 tttactattg aattaaaaga tgaattaatt gatgaaagta tgaaaggcaa atttaaaaat
      361 tggatgtttg gctgcgatat ttgccaagat gtttgccctt ggaatagatt tagtaaaccc
      421 accaacgaaa ttgcatttac acccatacca gaagtgttga atttaactac ccaacaatgg
      481 gaagaattaa gtgaagaaag tttcaagaaa atttttaaaa actctgcaat aaagcgaagt
      541 aaatacaaag gaattttaag gaatttaaaa tttttgaaac aataatttag ggtattaaag
      601 aaagaatttt ttcttctact tcattactat cccaaattgc ctctatattg ggtatttgca
      661 taatttgttg cataattttt tcttgaattt ggccacgttt taaagcatcg ctataacgaa
      721 tatttggaga aaaagtaact tgagcataag caggtatcca ttggttagga tatttttcat
      781 gtagtcttac ttctattttt ttctgtaata aaaacttagg gtcggctgtt ttagccctca
      841 tttcaataaa gttttcaata gctaaatctg caattgcatc tgcatctggt tttctagttt
      901 gttgaaaggc ctctaatata ttatgccaat tgttttcatt tgcctccatc aactcatcta
      961 aaattctaca atcctcaaaa ccacaattca ttccttgccc aaaaaatggc actacagcat
     1021 gagctgcatc tccaattaat gtaaacttat cgtctctaat ccaagggtaa cattttactg
     1081 taaccaacga ggaggttggg ttttcaaaaa agtcatctaa ataatttggc attaatttaa
     1141 tagcatctgg aaaagttttt tcaaaaaatg attttacttt ttctttagta tccaatttag
     1201 aaaaagaagt ttcgccctcg aatggaaaaa ataaagtgca ggtaaaagtt ttatctaaat
     1261 ttggtaagcc tattagcata taatcctttc ttggccaaat atgcaaggca tttacttcca
     1321 ttgcaaaatc tccttctttg gttggtggta tagttaactc tttataacca caatctatat
     1381 aatattgttg gtaatcaaat ttagaatggt gtacctgatg ttgcaacctt gtagcagcat
     1441 aagctccatc ggctccaaaa agtaaatcaa agtttgtagt aatggagttg ccattttcgg
     1501 tatcaaaatt tatttcattt ttgttccaat ttatattggc acatttatga ttaaaataaa
     1561 tcttcactcc ttcttgttcg gctaactcca tcaactttat attaagtgta gctctagaaa
     1621 cagagtttat aaattgtcct tctttaccat aaggttgaaa ggcagtagaa ccatctgcat
     1681 tatgaatata acgaccatgc attggtattg aaattttttt aatctcatct tctaacccaa
     1741 cttttttcaa agcaagcaat cctctatcac ttaatgctaa attaatagaa cgccctgctg
     1801 ataaagattc tttacgcata tctgaacggc gttcataaat agaaacagaa taccctcttt
     1861 ttgctaaata aattcctagt aaagagccta ccaaccctgc accagctata gtgatgttct
     1921 ttttttgcat acgctaaatt aaaatgaaaa aatataccgt tacaaatgta attcttagat
     1981 gttatttaat ttatattaat tatagcaagg ctctaatacc cgccctacca atatgtatca
     2041 tttttgaaga tgctggcttt ctaccctcat aattaatgtt aaattctaaa ttattgccta
     2101 acctttttgt aagctcaacc atccataaat aattttttcc tggcagcaaa gcatctaaca
     2161 taatataact tgtagttgta ccaatattgc cattaaaatt aatatgctgc atattaaact
     2221 ttgtaagtaa actagtgttg ttaaaaatgt tcagcttagc ctctgcttgt ataatgttat
     2281 taattgcttt ttcgcctcca taaataatat tgttttcttt ttgaacaaat atgtaggaga
     2341 gctgtaaacg ataacgagtt aaataagtat aagttaattt gggctccaca ctaaaacttt
     2401 taatattata gtttctatta ttaaaagatg gtgtaattaa agaagtagtt gaaaatttat
     2461 ttaatagttc caacgtatat acttttttga aatttattct tgttttaact aaccattcat
     2521 tcatccatct gctttcaaaa ccgtacgtta ataaggcttt tgatttattt tgaaaattgc
     2581 taacatctat accccaaata ttactatacc tattaaaaga aatggtatta cttaattgtt
     2641 gttgaatatg aattaaggta gtatctgtta tatttttttt aaatggatta aattgaattt
     2701 tattatctgc aataactttt tgagaggttt gtaatgatga ttgaaacatg aatttagaaa
     2761 taaatttttt aaacttttcg tttctaaatt tagccataat tgctttgggt gtaatttgta
     2821 aattgtaatt aaactgtaag taagatgctt ttacaaattg attagttgga gtaaatattc
     2881 taatatactt ggctaaatcc ggatataaag caatttcaaa ttcatttaat tgcggaatac
     2941 catctaaatt gtaatcattc catgtatatt ctcccctacc agctggtact tctatataag
     3001 taagatctct tttttgttct tggccagttc ctaattcgta cattacaaaa cctgttaaga
     3061 aaccattcca ttcatttacc ttatactctg ttcggctcaa taaagtttta tctggttgta
     3121 ggttggttaa aaaattattt tgaacaaata attgcctgta ggttgcatta aatttaagtt
     3181 gatgattatg gttgttcaat aaatgtaggc taaaattata gttattactt ctatctgttt
     3241 gtaataactg gctttgaaaa ggcagttcat tttttcgtgt aaaaaaatga aaagcccatt
     3301 gattattttt tctttcatta ctttttataa atgctcctat attttcaaaa gaaaaagaaa
     3361 taggctctaa gctagtagta aatttattgg attgtagatt tctttctaaa ctataattaa
     3421 agcccagttc ataattatta atttgtttta atactctgga aacttcagag ttaattttta
     3481 caaaatagcc tttactattt ggtgtaaaaa tatttaccag acttgaattt aattttatgg
     3541 aaattgcatt tttgggttga tattgataaa gtgaaacttg gttttttatt gcatagtatt
     3601 tgtcactcct taaataccca tttgttgtaa gctgtatttt attattattg tttaaatttt
     3661 ttaactcaag agatacttta ggtaaatgtt cagtagccaa gctggtaatt aatggcaagc
     3721 cccaatctct tgcaaattca actgtacgaa gcctttcaat aggtttgaat ttttcattaa
     3781 cccattcgta tccaatttta gtttgtagtt gatatttatt atggaaggta ttattatttt
     3841 gaagtaaaaa tttagcagca aatccaatat tgtcattttt atttttagaa gaaaaagtgt
     3901 ttacatcata attgctcaag gcaacatcgg taattaaagt tgtttttcta ttgatattgt
     3961 atattgcatt agcagtaaaa atttgttgct tttttggggt tactaaaaaa atagctggtt
     4021 caaaatttcc ttgttttaca ccattaatag gggcaatata ttgaaaaact cttccattag
     4081 cactattaaa aagaggtata taatttcctt tattaatgcc tacctcaata aagtttaaat
     4141 aatattttgc actatctgca tgggttgaat aaacataaat actatcccac aaagcattga
     4201 tggggtttac tcgttttgca tataaaattt ttgtattaga gaaagaatct aaaacaacag
     4261 aatgataaaa agcttgttca atgctatctc ctaaactatt taaaaattgt ttttgatttg
     4321 cattcagctg ttggttaatt ggagaatttt tagcatcgtt attactatat gcagctatat
     4381 taatttgtaa atttttattt actttcattt cctttgcagc ataaaccaaa tagtttaaat
     4441 aatttctatc ggcatattca aactcaacct gaattctttt atcttttgta atcatgtgct
     4501 ttggtgtaaa cacaatttcg gcagtgttat aatttataat ataatcctgg tcttcgcctc
     4561 tttgtaacat ttcaccatcc ataaaaactc tctctgtgcc cgccagaata attaaaaaaa
     4621 attcgttgtt attacctgct aatttataag gcccttgatt cccttctaac ccattaaaaa
     4681 tatttcttgc aaactttcct tttgcaatag cacctgcaaa agagaagttg ctatttttct
     4741 tactgtttaa cgatttatag ctagcacctt gtaacctttt ataaaaattc aggaaataat
     4801 attgattact tcttacatca atatctccta aatctaattg catatttctt ttcttaaact
     4861 gaagcaaaat tttatcaaat tcattcaatt gttgcgtagt tccatctggc tgaattggta
     4921 tattgttatc tgtaattgca gcagcaattt ctatgctatc tcccaacata ccacttatct
     4981 gcaaattcaa ttgtgagtta aaaactgcat cttgattatt tccaaagctt aaatttcttc
     5041 caaaacttcc attgtaatta atagatatag tttgaccaaa taaaccaaca ttttcatttt
     5101 gtttggattg gctgattact tgaggtgcag caataaaatt atttattaca ctatcataat
     5161 tgtatctata agcattctca tttatcttga aattaaaata ccggtattca attaaaacac
     5221 tatctgatgc tgttggcttt aaccaattaa taatattttt cgtataatca atactataaa
     5281 atgaactatc ataattataa attataactg tatgaggcac aatttttctg ccatcaaaag
     5341 caaacatact tttagaagca atttttttta caaccttatt agatgttgtt attatttgtg
     5401 cattagcaga gttaaacagt aacaatgcaa aaccaaaagc caatatattt agtaagtatt
     5461 tcaaaaataa agttggaaag taaaatttac atcatacaaa aattgtagta ataagttaag
     5521 tacaagatag tatttaggct taaacggctg cctaaattta taccagtaaa gttttataaa
     5581 ttatttacaa ttgtaataaa ttaagttatt taaacataaa aaatatacaa taaaaattca
     5641 gctttaattc tctaatctta ataaagttta aatcaatttt actaaactta acttatattc
     5701 gccgcataaa aataatctac catgaattta catgaatacc aagctaaaga attattaaaa
     5761 aagtacaatg tacctgtaca agaaggtata gctgtagaaa gtgtaatggc tgcagaagat
     5821 gcttacagac aaattaaaac acaaaccaat aacaattttg cagttattaa agctcaaatt
     5881 catgctggtg gcagaggaaa aggaaaagta aatgaaagcg gcttaaatgg agttaaagtt
     5941 gccaaaagtt tagaagatat tagcacttat gcaaaaggaa ttttaggtgg cactttagta
     6001 accatacaaa caggccctgc tggtaaaaaa gtaaacaagg ttttaattgc acaagatgtt
     6061 tattaccctg gcccaaatcc tgtaaaagag ttttatttat ctattttatt agatagagct
     6121 aaaggtcaaa acgtaatcat gtattctaca gagggtggaa tggatattga agaagtagca
     6181 cacaacactc cagaaaaaat atttaaagaa tgggtgcatc cttctggggg gttacaaggt
     6241 tttcaggctc gtaaaattgc atttaattta ggcttaagtg gcgatgcttt taaaagctgt
     6301 gtaaaatttg taactaatct ttacaatgct tatgtggggt tagattgttc tatgttagaa
     6361 ataaatcctt tatttaaaac aagcgatgat aaaattattg ctgtagattg taaaatggga
     6421 ttggatgaaa atgctttaat gcgtcatcct gatttagctg cattaagaga tattactgaa
     6481 gaagatccaa cagaagttga agcaggccaa tttaacctaa attatgtaaa acttgaaggc
     6541 aatgttggtt gtatggtaaa tggtgcaggc ttagcaatgg ctactatgga tatgattaaa
     6601 ttaagtggtg gcgaaccagc caatttttta gatgttggtg gtacagcaaa tgcccaaacg
     6661 gtagaagcag gttttaaaat tattttaaaa gacccaaatg taaaagctat attaattaat
     6721 atttttggtg gcattgttcg ttgcgatcgt gttgctgctg gtgttattga ggcttataat
     6781 aaattgggta atattaatat tccaattatt gttcgtttac aaggtacaaa tgctgcagaa
     6841 gctaaaaaat taattgatga aagtggctta aaagtacaaa gtgccatttt gttaagtgag
     6901 gcagccgatc ttgttaaaaa agcccttgca taattaaatt atttatacat gttttactac
     6961 cattagttaa aagctaatgg ttttttttga gactaccaat caaacaatta ttaattacca
     7021 ttttcaaaaa taatccctga gccataaggt tcagggatta taatgatact ttataaaagt
     7081 aattgaacta ttcttctact tttacttttc ctttattttt tgcaattact tcttctgcaa
     7141 tattattagg agcaggagtg taatgagaaa attccattgt agaagttgca cgtccagatg
     7201 ataaagaacg taactgtgta acataaccaa acatttcact caacggaact tttgctttaa
     7261 taacttgtag gttatttctg ctatccattc cttctaacat accacgtctt ctatttaagt
     7321 cacctgttac atcacccatg tattggtctg gtgttaatac ttccactttc ataattggtt
     7381 caagtaaaac aggttttgct tttctaccag cttcacggaa accagatttt gcacaaagct
     7441 caaaactcat tgaatcagaa tccacatcgt ggtaactacc atcaaaaaca cgaactttca
     7501 tattgtttac aggataacct gctaaaacac ctgttaccat tgatgtttca aaacctttaa
     7561 ttatagcagg tacaaattct tttggaattg aaccaccaaa taaatcattc acaaactgga
     7621 agcttttacc ttcattttct ttaatccact cttcatctgc tggcccaatt tcaaattgta
     7681 tatcagcaaa tttacctcta ccacccgatt gttttttcaa aacttctcta tgctcaattg
     7741 tagagtggaa agcttcttta taagcaactt gtggggcacc ttggtttact tcaaccttaa
     7801 actcacgtct taaacggtca ataataattt ccaaatgtaa ctctcccatt ccacgtaaaa
     7861 tagtttgtcc agtgtcttca tctgtattta cacgtaatgt aggatcctct tcaattagtt
     7921 tagcaattgc catacccatt ttatcaacat ctgcctgtgt tttaggctct attgcaattg
     7981 caattacagg ttctggaata aacatatttt ctaaggtaat aggtgctttt tcatcgcaca
     8041 aagtatcacc tgttttaatt tctttaaatc ctactgcagc agcaatatct ccagcttcaa
     8101 taaaatcaat tgggttttgc ttatttgcaa acattttcat tatacggcta atacgttcct
     8161 ttttaccact tcttacattt aatacataag aaccagcatc taaatgacca gaataacaac
     8221 ggaagaaagc caaacgacct acgaaaggat cggtcattat tttaaatgca agagccgaga
     8281 atggttcttt agcatctgct tttctaactt cttgttctcc agtatctggg ttagtaccaa
     8341 ttgtttcttc aatatctaca gggcttggca agtatctaca aacagcatct aaggcagttt
     8401 gtacaccttt atttttaaac gaagatccac acatcattgg cacaatgctt aaatcaatac
     8461 aagctttacg aattgcctcg tgaacttcct cttctgtaat agtttcaggg ttttcaaaga
     8521 atttctccat caacttgtcg tcgtactcag caactgcttc aattaaattt tgtctccaaa
     8581 atttcacatc ttccatcata tcttctggta ctggaatttc atcaaaagtc attccttctg
     8641 tttcagcatg ccaaacaata cctttcattt taattaaatc taccacacct ttaaaattat
     8701 cttcggcacc aataggtaat tgcaatggaa ctgattttgc tcccaacatt tcttttacct
     8761 gcttaactac atttaaaaaa tcggcaccag cacggtccat tttgttaaca aaaccaattc
     8821 ttggtacttt ataacggtta gcctgacgcc aaactgtttc agattgaggc tctacaccat
     8881 caactgcact aaataaggca attaaaccat ctaaaactct catactacgt tctacctcaa
     8941 ctgtaaaatc tacgtggccc ggagtatcaa ttatattaaa ataatatttt ttggtttcgg
     9001 gtgtagcttt tcctaaaata gtaggaaaat tccattgaca gctaaccgca gcagaggtaa
     9061 ttgtaatacc tctttctttt tcctgctcca tccagtcagt agttgcacca ccatcgtgta
     9121 cttctccaat tttatgaatc atacctgtat agcgtaaaat acgctcagta gtggtggttt
     9181 taccagcatc cacgtgagcg atgattccaa tgttacgtaa ctg
//
LOCUS       DAOQPK010000076         9196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_4043_length_9196_cov_4.550049, whole genome shotgun sequence.
ACCESSION   DAOQPK010000076 DAOQPK010000000
VERSION     DAOQPK010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 9196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9196
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4043_length_9196_cov_4.550049"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PLU36_05105"
     CDS             complement(<1..809)
                     /locus_tag="PLU36_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HMZ46161.1"
                     /translation="MEYRIEKDTMGEVKVPAHVLWGAQTQRSIENFKIAQDINKMPKE
                     IIEAFAYLKKAAALANNELGVLPNEKKEAIATVCDEILAGKLNDQFPLVVWQTGSGTQ
                     SNMNVNEVVAYKAHVNGGGSLLDEKKSIHPNDDVNKSQSSNDTFPTAMHIAAYKMLLQ
                     TTIPGIEKLRDTLAVKSKAFMQIVKIGRTHFMDATPLTLGQEFSGYTAQLNHGLKAIK
                     NTLAHLSELALGGTAVGTGINTPKGYDVAVAKHIAALTGLPFVTAENKFEA"
     gene            1047..1475
                     /locus_tag="PLU36_05110"
     CDS             1047..1475
                     /locus_tag="PLU36_05110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ46162.1"
                     /translation="MALKIIDHNSKQYKQMVDMRYNILRKPLGLAFSLEELEREKEDI
                     LIGCFEEDKLEGCCLLTKVDKNTLRLRQMAVLSGLQGKGIGRVLMIFAENIARDKGYH
                     KIIMHARKNSVGFYSKLGYSTVGNEFIEITIPHIIMEKEL"
     gene            complement(1566..2927)
                     /locus_tag="PLU36_05115"
     CDS             complement(1566..2927)
                     /locus_tag="PLU36_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ46163.1"
                     /translation="MSKKMIWIIVGLVLAIIILIGLKKAGIIGKEEGLKVAIEKVSKR
                     TIIETVTASGKISPEVEVKVSPDISGEIVELSVEEGDTVHRGQIVAKIYADIYASQRD
                     QAAAGVDQSKAAEANANASLAALDATLQQAYANYIRQKKLYDEKVISKAEYEQAEQTY
                     KTAQANYNAAKESIKGSKASIKSAQANLNRADKDISRTVITAPMNGVVNVLNVKKGER
                     VAGNSFSIGTEMMRIADLNSMVAIVDVGENDIPKVSYGDTAIVSIDAYGSRKFYGIVF
                     KIANPAASVTSTSSLNTEVANYKVHIRLLLDSYKDLIVKGKPFPFRPNMSATADIQTQ
                     TVKDVLSVPLAAVTTRDSKDNAIESKSKTKSSEESEEQDKTKTEEIEEVVFVKQADNK
                     IKKIKVTTAIQDLNYIQITSGLKENDEIIVGPYSLLSKTLKEGDLVKVVPKEQLYEAK
                     KQD"
     gene            complement(2946..4400)
                     /locus_tag="PLU36_05120"
     CDS             complement(2946..4400)
                     /locus_tag="PLU36_05120"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMZ46164.1"
                     /translation="MKYLLLVLATCIFYQSYAQTTSEKWNLQRMVDYAMKNNISVKQA
                     DIQARITALQHQSNKLAVYPNANFSSGLGMQFGRSVDPTTNQFTTTQLLFNNFNLSTG
                     ITLYNWGKLKNTISSSDFAVKASEAEIERAANDIALNVATYYLAVLSSKQQIEITLSQ
                     IASTNEQLENTRKRVDAGSLPELNAAELEARVAQDSINYYNAKSNFEQNILNLKGLLN
                     LDMAVPFEIEIPTLDKIPVLPLAELEPALVLQQALTELPQQKAANLRYKSAEYAVKAA
                     KAGMYPTITFGGGLGTNFASSTNKITGVTLGASQFTGQFVNVNGTDYYIQQPTVQFTQ
                     GKKGFGEMWDGYGVQLSNNFRQNVGFQINVPIFNNGSAKLNYQNSKLQLKQSEIAIEQ
                     SKQKLQQDIYMAHNNAIAALQRFNASEKTVETALRSFEYAKKRYEVGLITTLDLITNQ
                     NNYLQAKLTRLNNQFDYIFRMKLLEFYKGKGLKL"
     gene            complement(4454..5614)
                     /locus_tag="PLU36_05125"
     CDS             complement(4454..5614)
                     /locus_tag="PLU36_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HMZ46165.1"
                     /translation="MNISTIINEFAEERINYFNAVTPPIVQTSNFAFKSVEDLRNAFE
                     KEYDVWLYSRGNNPTVDILRKKLAALDGAEDCLVFNSGAAAIFASVLANVKSGDHIVS
                     VNGVYTWARKMFEQILPRFGVTHTYIDGTEIINFKKAIQKNTTIFYLESPNSWTFDLQ
                     DLKAVATLAKANNILTICDNSYCTALYQKPIELGIDLVLQSATKYIGGHSDVVAGVLT
                     GSKSMIEKIFYSEYMTIGSGIQPFNAWLLIRGLRTLTARMEKITTTTFQVVDYLLKHP
                     KVEEVVFPFNASFAQYELAKQQMKTVGGLLTFIIKVNNANQIEEFCNNLKHILMAVSW
                     GGYESLIIPKYATIAKNEFDSNNLWHKSLRLYVGLEEPDYIINDLEQAFSRL"
     gene            5732..6850
                     /locus_tag="PLU36_05130"
     CDS             5732..6850
                     /locus_tag="PLU36_05130"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HMZ46166.1"
                     /translation="MKNWVDLFIRQGDKQVIIKEIDGLRFIAIIAVVLSHFSLQIIKQ
                     SNLPVDFEYANGVALFLELGGYGVRIFFCISAFILSIQFIKYYLYNSEKVSLKKYYFK
                     RLKRLEIPYLLVLIILLLFRIFVQGEFWKDEMPHFFSSVFYSHNIIYDRRSTINPVAW
                     TLEIEIQFYLLLPFLMQIFLIKNFVKRRVLLVGCVLLFALLHSIFYHYITVAHLTYSI
                     VPYLPIFLLGILFADIYLSNKILLESKFFLWDVLGIIGFLIILYFAGYASFYKYIIEM
                     FGYVLLFTGIFKGRILNAIFTKKIWMAIGCMCYSIYLLHYALIAFIMQHITISIFKKG
                     YYNNLVLQGIIVLPLVLFICSIFYLIIEKPLTTKNRNI"
     gene            complement(6910..8025)
                     /gene="dnaN"
                     /locus_tag="PLU36_05135"
     CDS             complement(6910..8025)
                     /gene="dnaN"
                     /locus_tag="PLU36_05135"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HMZ46167.1"
                     /translation="MKFIVSSSSLLKHLQQINGVISANTVLPILEDFLFEIQDKKLSI
                     TATDLETVMRVQLDVESKTNGKVCIPAKILMDSLKNIADQPLTFSIDKNFAVEITSDN
                     GKYKVMGENPDNFPKEPVSDDATEFTMTSSALVTGINKSLFAVSTDDLRPAMTGVFFE
                     LKTDGVQFVATDAHRLVRYKRTDTKASKNDSFIVPKKPLTLLKGALPNNDDELSISYN
                     ASHLFVVHGNIQMTCRLIDARFPDYKVVIPAENPYHLTVNKDDFQSALRRVSVFSNKS
                     TNQVALSISSNELQMAAQDVDFSFEGNERMGCSYNGEDMQIAFNAKFLIEMLSAADTN
                     EVRLELSTPSKAGLIKPSEQEDGEDLLMLVMPLMLNN"
     gene            8238..8729
                     /locus_tag="PLU36_05140"
     CDS             8238..8729
                     /locus_tag="PLU36_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HMZ46168.1"
                     /translation="MAYSNKKFTPIQAYNKIQYYCSYQERSHFEVKEKLYGYGLNKLE
                     VETIIIQLIEENFLNEERFAIQFAGGKFRQKKWGKNKIVYELKNKRVSSRNIQIGINS
                     IDSLQYQNTLHKLALQKWNSLKKDSIIAKKVKTTKYLLQKGFEQSLIKEAIVKICLPD
                     NIN"
     gene            8758..>9196
                     /locus_tag="PLU36_05145"
     CDS             8758..>9196
                     /locus_tag="PLU36_05145"
                     /inference="COORDINATES: protein motif:HMM:NF021497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2219 family protein"
                     /protein_id="HMZ46169.1"
                     /translation="MRFFIFFLFIIVVFTSTSKAQDNKNYSREFLFMNENDIYLFMNK
                     DGYYTNGFLFQYSKLASSKKNKTIQRFSLGQNIYTVGDRRATWRWEAPFDRPYCGYLF
                     LKYTNDKFTTENTLFSTSAEVGVTGDWALGRQLQEWYHRVLSLF"
BASE COUNT         2951 a         1432 c         1340 g         3473 t
ORIGIN      
        1 aaggcttcaa acttattttc ggcagttaca aaaggtaatc ctgttaaagc tgcaatatgt
       61 tttgcaactg caacatcgta tcctttgggt gtattaattc cagtgcctac agcagtacct
      121 cctaatgcca attcacttaa atgagctagt gtatttttaa ttgcttttaa cccatgattt
      181 aattgagcag tataaccact aaactcttgc cctaaggtaa gaggtgttgc atccataaaa
      241 tgggtacggc caatttttac aatttgcata aaagctttac ttttgacagc caaagtatct
      301 cttagttttt caataccagg tatggttgtt tgtaacaaca ttttatatgc tgcaatatgc
      361 attgctgtgg gaaaagtgtc gttacttgat tgagatttat taacatcatc atttgggtga
      421 atagattttt tttcgtctaa caagctgcct cctccattta catgagcttt ataagcaact
      481 acctcattta cattcatatt gctttgtgta ccactaccag tttgccatac cactaacgga
      541 aattgatcgt ttaatttacc tgctaaaatt tcatcacaca ctgttgctat agcctctttc
      601 ttttcgtttg gcaaaacacc taattcgtta ttggctaaag cagctgcttt ttttaaatag
      661 gcaaatgcct ctataatttc tttaggcatt ttattaatat cttgtgctat tttaaagttc
      721 tcaatacttc tttgggtttg tgcaccccat aatacatgag caggaacttt aacctcaccc
      781 attgtatcct tttctatacg atattccata atatttaaaa tttgcagcaa aggtaagttg
      841 tgagtggttt aataataaag tgtgtttcta aaagtttctt gtttttatga actaattctt
      901 aactattttg ttcttttcta ttaaataata aatgcaataa tgttctctct tttagtaaaa
      961 tttttagaga aataataatg tttacaataa attttataaa atggtattaa aaagttattt
     1021 atcttgtaaa ctcctaacac ctgcttatgg ctttaaaaat aatagaccac aactctaaac
     1081 aatacaaaca aatggttgat atgcggtata atatattacg caaaccatta ggcttggcat
     1141 tttctttaga agaattagaa agagaaaaag aagatatttt aattggctgt tttgaagaag
     1201 acaaattaga aggctgttgc ttgcttacca aggtggataa aaatacttta agattaaggc
     1261 aaatggccgt tttaagtggc ttacagggga aaggtattgg cagggtttta atgatttttg
     1321 cagaaaatat tgcaagagat aaaggctacc ataaaataat aatgcatgca agaaaaaact
     1381 ctgttgggtt ttacagtaaa ttaggatata gtactgtagg aaatgaattt attgaaataa
     1441 ctatacctca tattattatg gaaaaagaat tgtagttgtt atgtatgcat cagttacata
     1501 acaactacaa tttagtgctg taaagagttt ttaaaaattt cttctaaatt tttaagctaa
     1561 agaaattagt cttgtttttt agcttcgtat aattgttctt taggaactac tttaactaaa
     1621 tcgccttctt ttaaggtttt gcttaataag ctgtaaggcc caacaataat ttcgtcattt
     1681 tcttttaagc cgcttgtaat ttgtatataa ttcaaatctt gaattgcagt ggtaactttt
     1741 atttttttta ttttattatc tgcttgtttt acaaacacta cttcttctat ttcttctgtt
     1801 tttgttttat cctgctcctc tgattcttct gaagatttgg tttttgattt gctttcaata
     1861 gcattgtctt tactatctct tgtggtaaca gcagccaatg gaactgataa aacatctttc
     1921 actgtttgtg tttgaatatc tgctgtagca ctcatattgg ggcgaaaagg aaatggttta
     1981 ccttttacga ttaaatcttt ataactatcc aacaataatc ttatatgtac tttataattt
     2041 gcaacttcag tatttaaaga agatgtggaa gttacacttg ctgcggggtt ggcaatttta
     2101 aaaacaattc cataaaattt tctactacca taagcatcaa tgctaacaat tgcagtatcg
     2161 ccatagctaa ctttaggtat gtcgttttcg cctacatcta caatagcaac catgctattc
     2221 aaatctgcaa tacgcatcat ttctgtacca atactaaagc tatttcctgc tacacgttct
     2281 ccttttttaa cattcaatac atttaccact ccattcatag gagctgtaat aacagttctg
     2341 cttatatctt tatctgcacg gtttaaattg gcttgtgcac tttttataga agccttactg
     2401 cctttaatac tttctttagc tgcattataa ttggcttgag ctgttttata agtttgttcg
     2461 gcttgttcat actctgcttt tgaaattact ttttcatcat atagtttttt ttgacgaata
     2521 taatttgcat aagcttgttg taatgtggca tctaatgcag ccaaacttgc attggcatta
     2581 gcttctgctg ctttactttg atctacacct gcagcagcct gatctctttg agaagcataa
     2641 atatctgcat aaatttttgc aactatttgc ccacgatgta cagtatctcc ttcctccaca
     2701 cttaattcta caatttcgcc acttatatct gggctcactt tcacttccac ttctggcgaa
     2761 attttgccac ttgctgttac agtttcaatt attgttcttt tagaaacttt ttcaatagct
     2821 acttttaagc cttcttcttt acctattata ccagcttttt ttaatcctat taaaattata
     2881 attgccagta ctaaacccac aattatccat atcatttttt tactcatact acttcatttt
     2941 tgtatttata gtttcaatcc tttcccttta taaaattcta ataatttcat tctaaaaata
     3001 taatcaaact ggttatttag ccttgtaagt tttgcttgta aataattatt ttgattggtt
     3061 attaaatcta aggttgtaat taaacctact tcatatcttt tcttagcata ttcaaaactt
     3121 ctaagtgctg tttcaactgt tttttcgctt gcattgaaac gttgtaaggc agctatggca
     3181 ttgttatgtg ccatataaat atcttgttgt aatttttgtt tagattgttc aatggcaatt
     3241 tctgattgct ttaattgtaa tttgctattt tgataattta gttttgcact gccattatta
     3301 aaaatgggta cattaatttg gaagcctaca ttttggcgaa aattattgct taattgtaca
     3361 ccataaccat cccacatttc accaaaacct tttttgcctt gtgtaaactg tacagtaggt
     3421 tgttggatat aataatctgt tccgtttaca ttaacaaact gacctgtaaa ttggcttgca
     3481 cctaaagtaa cacctgtaat tttattggta ctgcttgcaa aatttgtacc taagccacca
     3541 ccaaaagtaa tagttgggta catacctgct ttagcagcct taacagcata ctctgcactt
     3601 ttgtatctta agttggcggc tttttgctga ggtaattctg ttaatgcttg ttgtaatact
     3661 aatgcaggct ctaattcagc caatggcaat acaggtattt tatctagtgt tggaatttca
     3721 atttcaaaag gcactgccat atctaaattc aataagcctt ttaagtttaa aatattttgt
     3781 tcaaaattac tttttgcgtt ataataatta atactgtctt gtgctactct tgcttctaat
     3841 tctgcagcat ttaattctgg caatgagccg gcatctactc tttttcttgt attttctaat
     3901 tgttcattgg tagatgctat ttgtgaaagg gtaatttcaa tttgttgttt actgcttaaa
     3961 actgctaaat aataagtggc aacatttagt gcaatatcat tggctgctct ttcaatttct
     4021 gcttcagagg cttttactgc aaaatctgag gaactgatgg tattttttaa ctttccccag
     4081 ttatacaaag taataccggt acttaaatta aaattattaa aaagtaattg tgtagtagtg
     4141 aactggttgg tggtaggatc tactgaacgt ccaaactgca ttcctaaacc tgaactaaaa
     4201 ttggcattag ggtaaacagc taacttattt gactgatgct gcaaggctgt aattctggct
     4261 tgaatatctg cttgttttac agatatatta tttttcattg cataatcaac cattctttgc
     4321 aaattccatt tttcggaagt tgtttgtgca taagattgat aaaaaataca agtggctaaa
     4381 acaagtaata aatatttcat ttaaacaaaa ataatagaaa gtagttaacc ctttttaaat
     4441 tttaactcaa aaattataaa cggctaaatg cttgttctaa atcattaatt atatagtcag
     4501 gttcttctaa acctacatac agcctaaggc ttttatgcca taaattgtta ctatcaaact
     4561 cattttttgc aattgtagca tatttaggta ttattaaact ttcgtaacca ccccagctta
     4621 ctgccattaa aatgtgtttt aaattattac aaaactcttc aatttgatta gcattattaa
     4681 ctttaattat aaaagttagc aggccaccta ctgttttcat ttgttgtttt gctaactcat
     4741 attgagcaaa tgatgcatta aaagggaaaa ccacttcttc tacttttggg tgctttaata
     4801 aataatcaac tacctgaaat gtggttgttg taattttttc cattctggca gtaagtgttc
     4861 ttagccccct aattagtaac catgcattaa aaggttgaat tccgctacct atagtcatat
     4921 attcactata aaatattttt tcaatcatgg atttgctgcc tgttaaaacg cctgcaacca
     4981 catcgctatg gccaccaata tattttgtag ctgattgtaa tactaagtct attccaagtt
     5041 ctattggttt ttggtataaa gctgtgcagt aactgttatc gcaaatggtt aatatattat
     5101 ttgctttagc caaagtagct actgctttta aatcttgcaa atcaaacgtc cagctatttg
     5161 ggctttctaa ataaaaaata gtagtatttt tttgaatagc ctttttaaag ttgataattt
     5221 ctgtaccatc aatgtaagta tgtgtaactc caaaacgtgg caaaatttgc tcaaacattt
     5281 ttcttgccca agtgtaaacg ccattaacac taacaatatg gtcgccagat ttaacattag
     5341 ccaaaacaga agcaaaaatt gctgctgcac cactgttaaa aacaaggcaa tcctctgcac
     5401 cgtctaaagc tgctaatttt tttcttaaaa tatctaccgt tgggttattg cctctgctgt
     5461 acaaccaaac atcatattct ttttcaaatg catttcttaa atcttccaca gacttaaagg
     5521 caaaattgga tgtttgaaca attggtggcg ttactgcatt aaaataattt atcctttctt
     5581 ctgcaaactc atttataatt gtagaaatat tcatacagta ttaaatttat tagttttaga
     5641 atagaaataa ttgaatactc aacctaatta atctatttct taaatgtaaa aattatcagc
     5701 ctaaaataca aataatgtta cttttatatt aatgaaaaat tgggtagatt tatttataag
     5761 gcaaggagat aagcaagtaa ttataaagga aattgacggg ctacgattta ttgcaattat
     5821 agctgtagta ttaagccact ttagtttaca aattataaag caaagcaact tacctgtaga
     5881 ttttgaatac gccaacggtg tagctctttt tttagaatta ggaggttatg gtgttagaat
     5941 atttttttgt atcagtgcat ttattttatc tattcagttt ataaaatatt atttatataa
     6001 ttccgaaaaa gtgagcttga aaaaatatta ttttaagaga ttaaagcgtt tagaaatacc
     6061 ttatttgctt gttttaatta ttttactttt atttagaatt tttgttcaag gagagttttg
     6121 gaaggatgaa atgcctcatt tttttagcag tgttttttat agtcataata taatctatga
     6181 cagaagaagt accattaacc ctgttgcctg gacattagag attgaaattc agttttatct
     6241 tttattgcca tttttgatgc aaatatttct tataaagaat tttgtaaaaa gaagagtact
     6301 tttagtggga tgtgtgcttt tatttgcttt actacattct attttttacc actatataac
     6361 ggttgctcat ttaacctact ctattgtacc ctacttgcct atttttttgt taggtatttt
     6421 atttgctgat atttatttaa gtaataaaat tttattagaa tctaagtttt ttttatggga
     6481 cgttttaggt attataggct ttttaattat cctctatttt gcaggatatg ctagctttta
     6541 taaatatatt attgagatgt ttggatatgt tttattattt acaggcattt ttaaaggcag
     6601 aattttaaat gcaattttta ctaaaaaaat atggatggct attggttgta tgtgttattc
     6661 aatttattta ttacactatg ctttgatagc ttttataatg caacatatta ccatttcaat
     6721 atttaaaaaa ggatactaca ataatttagt tttacaaggt attattgtgt tgccattagt
     6781 tttatttatt tgcagtattt tttatttaat aattgaaaaa ccgctcacta caaaaaatag
     6841 aaatatttga aataactaaa taaaaaaaga gaggttggaa gcctctctta atatttagaa
     6901 aatataaaat taattattta acattaaagg cattactaac attaacaaat cttcaccatc
     6961 ttcctgttca gatggtttaa ttaagcctgc ctttgatggt gtacttaact ctaaacgtac
     7021 ttcattggta tctgcagcac tcaacatttc tatcaaaaat ttagcattaa atgctatttg
     7081 catatcctct ccattgtatg agcatcccat tctttcatta ccttcaaaac taaaatctac
     7141 atcttgagca gccatttgta attcgttgct gctaatgctt aaagctactt gattagtact
     7201 tttattacta aaaacactaa ctcggcgtaa tgcactttga aaatcatctt tattaacagt
     7261 taaatggtaa ggattttctg caggaattac aactttgtaa tcaggaaatc ttgcatcaat
     7321 taatctacaa gtcatttgta tattaccatg taccacaaat aaatggcttg cattatagct
     7381 aatacttagt tcgtcgtcat tatttggcaa agcacctttt aaaagagtta atggtttttt
     7441 gggaacaata aacgaatcgt ttttagaagc ttttgtatct gttcttttat atcgtactaa
     7501 tctatgagca tcggtggcaa caaactgtac cccatctgtt tttagttcaa aaaacacacc
     7561 agtcattgca gggcgtaaat catcggtact tactgcaaat aaagatttat tgatacctgt
     7621 aactaaggca gaactagtca tagtaaattc tgtagcatca tcagatactg gttctttagg
     7681 aaaattatct ggattttctc ccatcacttt atactttcca ttatcgctgg taatttctac
     7741 agcaaaattt ttatcaatgc taaaagttaa aggttggtct gcaatattct ttaacgaatc
     7801 cattaatatt tttgcaggaa tacatacctt accatttgtt ttactttcca catctagttg
     7861 aactctcata acagtttcta aatcggttgc agtaatgctt aattttttat cctgaatttc
     7921 aaataaaaaa tcttccaaaa ttggtaaaac agtattggca cttataacac catttatttg
     7981 ttgtaaatgc tttaataaag acgaagatga aacaataaat ttcataataa atatttttta
     8041 tgatagcata tccgattttt tcggcttgga gcaaacctaa tgtaaattga ttaatttttg
     8101 tagaaattat gttcctcgta aagttttatt cacaacattt gggctattat gtgaattttg
     8161 ttaggaactt atctaaataa agaatatttt aaataagttc tatcttttat tttttacaga
     8221 ttctaaaaac gataaacttg gcttatagca acaaaaaatt tacccctata caagcctaca
     8281 ataaaataca atattattgt agctatcaag aacgcagcca ctttgaagta aaagaaaaac
     8341 tgtatggcta tggattaaat aagttagaag tagaaactat tattatacaa cttattgaag
     8401 aaaatttttt aaatgaagaa aggtttgcca ttcaatttgc aggtggtaaa ttcagacaaa
     8461 aaaaatgggg gaaaaataaa atagtgtatg aacttaaaaa caaaagggta agtagtagaa
     8521 atattcaaat tggaattaat tcaattgata gtttacaata tcaaaacacc ttacacaaat
     8581 tagctttaca aaaatggaac agtctgaaaa aagattcaat tattgccaaa aaagtaaaaa
     8641 caacaaagta tttattacaa aaaggatttg aacaaagttt aattaaggag gcaattgtaa
     8701 aaatttgttt accagataat attaactaat tatattgcat ggctgctaaa tttgattatg
     8761 agatttttta ttttttttct atttattata gttgttttta caagtacaag taaggctcag
     8821 gacaataaaa attatagtcg tgagttttta tttatgaacg aaaatgatat atatcttttc
     8881 atgaataaag atggttatta taccaatggt tttttatttc aatacagcaa attagcaagt
     8941 tctaaaaaaa ataaaacaat tcaacgtttt tctcttgggc aaaacattta tactgtagga
     9001 gatagaaggg caacatggcg ttgggaagcc ccatttgata gaccctattg tggctactta
     9061 tttttaaaat atacaaacga taagtttaca acagaaaata ctttattcag cacttctgct
     9121 gaggttggtg taacaggtga ttgggctctt ggaagacagt tgcaagaatg gtatcatagg
     9181 gttttaagct tattta
//
LOCUS       DAOQPK010000077         8892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_4257_length_8892_cov_4.608012, whole genome shotgun sequence.
ACCESSION   DAOQPK010000077 DAOQPK010000000
VERSION     DAOQPK010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8892
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4257_length_8892_cov_4.608012"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PLU36_05150"
     CDS             <1..258
                     /locus_tag="PLU36_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46170.1"
                     /translation="KDVLVNEDIVEISCANNEKLVEIMKLFPEIQNIQIEKDKLLLYF
                     LLGKANLEAINQYCFSNNIVLNLLTMKHKSLETKFFELTNN"
     gene            639..3746
                     /locus_tag="PLU36_05155"
     CDS             639..3746
                     /locus_tag="PLU36_05155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ46171.1"
                     /translation="MSICMMAGIATAQSGRTVTGVVKDVNGKPVADASVVVKGTTIGT
                     TTDSKGMFTLTSVPASAKILVVSSINFETTDVSIGNGVVSVTLKASEDDLGEVVVTVP
                     YGTVKRTAFTGSSNTVSASTINKQQVTSVSRVLEGLIPGLQATNGGGAPGSGAAVRLR
                     GIGSVNASSSPLYVVNGVPYDGSISAIASEDIESIDVLKDAAAANLYGSRAANGVIMI
                     TTKRGKGAPSLQGSIRHSFSSRGIPEYDRVGAADYYELFWEAYRNSYVYGSGQTMAQA
                     GVNASNILTGSSGLVYNAYNVPGNTLVDPVTGKLNPNAQLLWNESWSDALFRTAQRTN
                     ANVSISGSSDKANYYISGGYLNEEGAAKFSGYKRYTFRASVDIKATKYLKAGINIDGA
                     SDRRDGLFAGGTATSNPFYYSRQMGPIYPVYERNLTTGAIVKDANGNPVLDFGTTAQM
                     GSRPYAANSNLVGTLALDKRQQKRLNGNFNPYIEISFLKDFTFRTQMGLNYYAIDALT
                     YQNNQYGDAANVQGRSTVSNDYQFSLTGNQVIKWAKNFKKHNVSALAGHENYKYKSRS
                     MSATKTGFSYPGQTELDNGTVVESPPSSSEDLQTMESYFGGVNYSYDDKYLASASIRT
                     DGSSRFASDVRWGSFYSFGLGWRISQESFLKNVNWIDEMKIKLSYGESGNENIGYFYQ
                     YKDWYYADGLGNFAPPTRLANKDLKWEKNRALNYGVEFTMFKRRLQGSIELYDRRSSD
                     LIFGRPIEPSVGAGNDVLENVGQVKNTGIEIQLGYNVVRKKNFDWRVDFNISSFKNVI
                     TELPKAQREKGIISGTKKMMEGKSVYDFWLREFAGVSAATGRALYYRDVLDNNGKPTG
                     NRVITDDATLASFYYVGGSAIPKFMGGITNSFRYKNFDFSFLMTYSYGGRFYDGNYAS
                     IMHYGSAGTALHVDAKNRWQKPGDITNVPRLQNASGQETSSSRFLLDASYLSIKNVTL
                     SYSLSNNVAKKIHLNGASFFVNVDNAYIFTAKKGSDPQQSFDGVTGAGYPPFRTFTLG
                     ATIKL"
     gene            3767..5257
                     /locus_tag="PLU36_05160"
     CDS             3767..5257
                     /locus_tag="PLU36_05160"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HMZ46172.1"
                     /translation="MKNKSLSLLLVAAIATTVGCKKGYLDTAPSNAISLEQAYATIAA
                     INSGLTATYNSTFDFAVGNGNTGHDNYGQKSCDLANDLMGQDMIVHSQGYGWYNRDYQ
                     YVEWTLPNGTGTRSDKVWSFYYNVIRQVNLLLDNTKNQAVTPDLESARGQAYGIRAWA
                     YFNLINNFQQTYKGNETAKGVPLFIEPNATSKPRGTVQQVYDQIIADLTQAETLLNGK
                     PRASKMNMDVSVIRGLRARVALVQEDWTAAANYANLAKQPYTLMSNAVYTSRTGFSTI
                     GTSEWMWGTLITAAQATIYASFFSHMDPSQLSYAQLGGQKKIPKALYDLIPASDARKQ
                     VFTAPGTGTANFPDYCQKKHLVPTIGSWAADYLYMRASEMYLIEAEALARKGGAEAQA
                     QAVLQAMVGLRNPTYNAAAFTGTALINEILLQRRIELWGEGFSLFDIKRTHSGLNRAT
                     GAGNHGAPNFNPIVYTQPDASPLFLMRIPQRELDNNTAMTAADQNP"
     gene            5300..6163
                     /locus_tag="PLU36_05165"
     CDS             5300..6163
                     /locus_tag="PLU36_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46173.1"
                     /translation="MKLKLNIKTARNLAIASLAVISFSSCKKNNIAVDVDALNTPDAP
                     ALAINAASRTAMYYVSPTSAPYKIPVTVMNVSTGDRAFNIAYTSNTATAGVQYSAPST
                     VTVKAGSVGDSLAVQGIFAGLTAAKDTVKIKFSGTTGLYAKDSFMLVLLKSCPVVLTN
                     MSGAFPGTEEYNSAGVKQWGPYTVDVLNLVAGGSPNTATGQFDGLYDFDWGPINFTMD
                     YSNINPGLWTVTIPLQTTGSTGSGVTHVRGSAGKTNTFSACDQTFTITLDLMTDATTV
                     GAAAYQFRIKR"
     gene            complement(6239..7291)
                     /gene="galE"
                     /locus_tag="PLU36_05170"
     CDS             complement(6239..7291)
                     /gene="galE"
                     /locus_tag="PLU36_05170"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HMZ46174.1"
                     /translation="MPTILVTGGCGYIGSHTIVDLIENNFDVISIDDNSRSTNIAIDG
                     IQKITGKRIKNYKVDLKDFNDTRAVFQENQHIDGIIHFAAYKAVGESVEKPLLYFENN
                     MVGLINLLKCVSEFNIPHFVFSSSCTVYGNPDNIPVTEKSPMKEAESPYGATKQMGEQ
                     MIKDFSKVNNISSILLRYFNPVGAHPSTYIGELPIGKPMNLVPAITQTAIGKISTMFV
                     HGNDYDTRDGSCIRDYIHVCDIAHAHTLGIQYLLDKRNRTNCDIFNLGTGCGVTVLEA
                     IRTFEEVSGLKLNYEIGPRRLGDVVAIFANNELAIQKLNWQIKYDLNAMLKTAWDWEL
                     KLKEEEDLLHNQTLLN"
     gene            complement(7329..8375)
                     /locus_tag="PLU36_05175"
     CDS             complement(7329..8375)
                     /locus_tag="PLU36_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HMZ46175.1"
                     /translation="MDFPVLLIVIIALAFIFDYINGFHDAANSIATIVSTKVLTPFQA
                     VLWAAVFNFAAFFVSKYLIGEFKIGNTIAKSVHENYITLEVIFAGLMAAITWNLITWW
                     FGIPSSSSHTLLGGFIGASLAYAGAFSKNGIDVINYAKVLPTVLFIFIAPLIGMFIAF
                     LITILIVHIFKRSNHYKAESWFKKLQLASSAAFSLGHGGNDAQKVMGIIGAAIIYHES
                     TLGHSLSFNEFVIKYSWVPFCSFFAIGLGTLSGGWKIVKTMGTRITKVTPLEGVSAET
                     AGAVTLYISEHLGIPVSTTHTISGSIIGVGATKRLSAVRWGVTVNLLWAWILTIPISA
                     LMAAGFYYILKLFV"
     gene            complement(8420..>8892)
                     /locus_tag="PLU36_05180"
     CDS             complement(8420..>8892)
                     /locus_tag="PLU36_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HMZ46176.1"
                     /translation="KNDDATHLIFTELGRNFITPFDREDIHYLATALDDIADYIYASS
                     KKISFYKVNPNDSGIQKLAELVLQGTTEISKAVKGLRDMKHLREMTEALVKVNSIENQ
                     ADDVFDMSIEMLFNQENDFKEVIKKREIYQALETATDKCEDAANVIESIIIKYS"
BASE COUNT         3030 a         1411 c         1498 g         2953 t
ORIGIN      
        1 aaagatgtat tggtaaatga agatattgtt gaaatttctt gtgctaataa tgaaaaatta
       61 gtagaaataa tgaaactctt tcccgaaatt caaaatatac aaatagaaaa agacaaactg
      121 ctactttatt ttttacttgg taaagcaaat ttagaagcta taaatcaata ttgttttagt
      181 aataacattg tgttaaactt acttacaatg aagcataaaa gtttagaaac taagtttttt
      241 gagcttacca ataattaatt ttttaaattt tcaagaaata atctccaata aattttgaga
      301 tggcaaaatg cctaatagag agttttttta tctttagatt tgtttcagca tttcttttta
      361 taaattatta atttaatgtg taatatgtta taaatgaata tattgattat agatttattt
      421 attttcacca aattgcagca aaataataga aagcttgtct tattagtaat gttaaagact
      481 tgttatctaa atttggtaac ataaatcatc gtattaagga aatattttac aaaaggcttt
      541 gttatttaaa ataaatgtta aatatttgtc gcaagacgat ggtttattaa acgtttaaaa
      601 aaatgaaaca atttatgaaa aaactcgctt ttctgcttat gagtatctgc atgatggcag
      661 gtattgcaac agcacagtca ggcagaactg taacgggtgt cgtaaaagat gtaaacggca
      721 aacccgtagc tgatgcatcg gtagtagtta aaggtactac cattggaaca acaactgata
      781 gtaaaggtat gtttacactt acttcagtgc ctgcttctgc aaaaatttta gtagtttctt
      841 ctattaattt cgagactact gacgtgtcaa ttggtaatgg ggttgtttca gttactttaa
      901 aagcatctga agatgattta ggtgaagttg ttgtaactgt accttatggt actgtaaaaa
      961 gaactgcatt tacaggttct tcaaacacag tatctgcctc taccattaac aaacaacagg
     1021 taacttcagt atcaagagta ctagaaggct taattcctgg tttacaagct accaatggtg
     1081 gtggtgctcc aggttctggt gctgctgttc gtttaagagg tattggttct gttaatgctt
     1141 catcttctcc attatatgta gtaaatgggg tgccttatga tggttcaatt tctgcaattg
     1201 cttcagaaga tattgaatct attgatgttt taaaagatgc tgctgctgca aacttgtatg
     1261 gctctagagc tgctaatggt gtaattatga ttacaactaa aagaggtaaa ggtgctccaa
     1321 gtttacaagg aagtatcaga catagcttct ctagcagagg tattcctgaa tatgacagag
     1381 taggtgctgc agattattat gaattatttt gggaagctta cagaaactct tatgtttatg
     1441 gaagtggtca aactatggct caagctggtg taaatgcttc aaacatttta actggttctt
     1501 ctggcttagt ttataacgca tataatgtac ctggtaatac tttagttgac ccagttactg
     1561 gtaaattaaa tcctaatgct caattacttt ggaacgagtc atggtctgat gctttattta
     1621 gaacagctca aagaacaaat gctaacgtta gcatttctgg ttcttctgat aaagctaact
     1681 actatattag tggtggttac ttgaatgaag aaggagctgc aaaattctct ggctataaaa
     1741 gatatacttt tagagcttca gtagatatta aagcaacaaa atacttaaaa gctggtatta
     1801 atatagatgg tgcttctgat agaagagatg gtttgtttgc tggtggtact gcaacatcaa
     1861 atcctttcta ttacagccgt caaatgggtc caatttaccc tgtatatgaa agaaacttaa
     1921 ctacaggtgc tattgttaaa gatgctaatg gcaacccagt tttagacttt ggtacaactg
     1981 ctcaaatggg ttctcgtcct tatgctgcca actctaactt agttggaact ttggctttgg
     2041 ataaacgcca acaaaaaaga ttaaatggaa actttaaccc atacatagaa attagtttct
     2101 taaaagattt cacttttaga actcaaatgg gcttaaacta ctatgcaata gatgcattaa
     2161 catatcaaaa taaccaatat ggagatgctg caaacgtaca aggtagatca actgtatcta
     2221 acgattatca gttttcttta actggtaacc aagtaattaa atgggctaaa aactttaaaa
     2281 aacacaatgt aagtgcttta gctggtcatg aaaattacaa atacaaatca agaagtatgt
     2341 ctgcaacaaa aactggtttc tcttacccag gtcaaactga gttagataac ggtacagttg
     2401 ttgaatctcc accatcttct agcgaagatt tacaaacaat ggaaagttat tttggtggtg
     2461 taaactattc ttacgatgat aaatatttag cttctgcaag tataagaaca gatggttctt
     2521 caagatttgc ttctgatgtt agatggggtt cattctattc atttggttta ggttggagaa
     2581 tttctcaaga atctttcttg aaaaatgtaa actggattga tgaaatgaaa attaaattaa
     2641 gctatggtga gtctggtaat gaaaacattg gttatttcta tcaatataaa gattggtatt
     2701 atgctgatgg attaggaaac tttgctcctc ctacaagatt agcaaataaa gatttgaaat
     2761 gggagaaaaa ccgtgcttta aactatggtg ttgaattcac tatgtttaaa agaagattac
     2821 aaggatctat tgaattatat gacagacgtt catctgactt aatttttggc cgtccaattg
     2881 aaccatcagt tggtgctgga aatgatgtat tagaaaatgt tggtcaggtt aaaaatactg
     2941 gtattgaaat tcaattaggt tataatgttg ttagaaagaa aaactttgat tggagagtag
     3001 attttaatat ctcttctttc aaaaacgtaa ttacagaatt accaaaagca caacgtgaaa
     3061 aaggtataat ttctggaaca aagaaaatga tggaaggaaa atcagtatat gatttttggt
     3121 tgagagaatt tgctggtgtt agtgcagcaa ctggtagagc attatactat agagatgtat
     3181 tagacaataa tggtaaacca acaggaaaca gagtaataac tgatgatgca actttagctt
     3241 cattctacta tgttggtggt tctgctattc ctaaatttat gggtggtatt accaattcat
     3301 tccgttataa aaacttcgac ttctctttct taatgactta ttcttatggt ggtcgttttt
     3361 acgatggaaa ctatgctagc attatgcatt acggaagtgc aggtactgca ttacatgtgg
     3421 atgctaaaaa tcgttggcaa aaacctggag atataacaaa tgtacctcgt ttacaaaatg
     3481 cttctggtca agaaacatct tcatcaagat ttttattaga tgcttcttat ttaagtatta
     3541 aaaacgtaac attaagttat agtttatcaa ataatgttgc taaaaaaatt catttgaatg
     3601 gtgcttcttt ctttgtaaat gtggacaatg cttacatttt cactgctaaa aaaggtagcg
     3661 accctcaaca aagttttgat ggagttactg gtgcaggata tcctccattc agaacattta
     3721 cattaggtgc aacaattaaa ttataaaaaa ttttaaaaat ttgaatatga aaaataaatc
     3781 tttaagctta ctacttgttg ctgcaatagc tacaacagtt ggttgtaaga aaggttattt
     3841 agatactgct ccttctaatg ccatttcatt ggagcaggca tatgctacaa tagcagctat
     3901 caactctggc ttaactgcaa cttacaattc aacttttgat tttgcagtag gtaatggaaa
     3961 tactggtcat gataactatg gtcaaaaatc ttgcgattta gctaatgact taatgggaca
     4021 agatatgatt gttcactctc aaggttatgg ctggtataac agagattatc agtatgtaga
     4081 gtggacttta ccaaatggta caggtacacg ttctgataaa gtttggtctt tttattataa
     4141 tgtaattaga caagtaaact tattattaga caacactaaa aatcaagctg taactccaga
     4201 tttagaaagt gcaagaggac aagcttatgg tattagagct tgggcttatt ttaacttgat
     4261 taataatttt caacaaacct ataaaggaaa tgaaactgca aaaggagttc ctttatttat
     4321 tgaaccaaat gcaacttcaa aaccaagagg tacagttcaa caagtttatg atcaaatcat
     4381 tgcagattta acacaagccg aaactttatt aaacggtaag cctagagcat ctaaaatgaa
     4441 tatggatgta agtgttataa gaggtttacg tgcaagagtt gctttagtgc aagaagattg
     4501 gactgctgca gctaactatg ctaatttagc taagcagcct tatactttaa tgtctaatgc
     4561 tgtatatact tcaagaacag gattttcaac aattggtaca agtgagtgga tgtggggtac
     4621 tttaattacc gctgcacaag caacaattta cgcttctttc ttctctcaca tggacccttc
     4681 tcaattaagc tatgcacaat taggaggtca gaaaaaaata ccaaaagcat tatacgattt
     4741 aattcctgct tctgatgctc gtaaacaagt atttactgca ccaggtactg gtactgcaaa
     4801 tttccctgat tattgtcaga aaaaacactt agtaccaaca attggtagtt gggcagctga
     4861 ttacttgtac atgagagctt ctgaaatgta tttaattgaa gcagaagcat tagctagaaa
     4921 aggtggtgct gaagctcaag cacaagctgt attacaagca atggttggct taagaaaccc
     4981 aacttataat gctgcagcct ttacaggtac tgctttaatt aatgaaattt tattacaaag
     5041 aagaattgaa ttatggggtg aaggtttctc tttatttgat atcaagagaa cacactctgg
     5101 tttaaaccgt gcaactggtg caggtaacca tggtgctcct aactttaatc caattgtata
     5161 tacacagcca gatgctagtc cattgttctt aatgagaatt cctcaaagag agttggataa
     5221 taatactgca atgacagcag ctgaccaaaa tccttaattt attttgtatt aaataaataa
     5281 aacattaaaa ctttaaagaa tgaaacttaa attaaatatt aaaactgcaa ggaatttggc
     5341 aatagcatca ttagctgtta ttagctttag ttcttgtaag aaaaataata ttgcagtaga
     5401 tgtagatgca ttaaatacgc ctgatgcccc tgcattggct attaatgctg catctagaac
     5461 agcaatgtat tatgtttctc caacatctgc accatacaaa ataccagtta cagtaatgaa
     5521 tgtaagtact ggagatagag catttaatat tgcatacact tcaaacactg caactgctgg
     5581 tgttcagtat agtgcacctt ctactgtaac agttaaagct ggctcagtag gagattcttt
     5641 agcagtacaa ggtatttttg ctggtttaac tgcagcaaaa gatactgtta aaattaaatt
     5701 tagtggtaca acaggtttgt atgctaaaga ttcttttatg cttgtattgt taaaatcttg
     5761 ccctgtagta ttaactaata tgtctggtgc ttttcctggt acagaagaat acaatagtgc
     5821 tggtgtaaaa caatggggcc catatactgt agacgtttta aatttagttg caggaggttc
     5881 tcctaacact gctacaggac aatttgatgg tttatatgac tttgattggg gcccaattaa
     5941 ctttactatg gactatagta atataaatcc tggattatgg acagttacta taccattaca
     6001 aacaacaggc tccacaggta gtggagtaac acatgttaga ggttctgccg gtaaaacaaa
     6061 cactttctca gcatgcgatc aaacttttac cataacttta gatttaatga cagatgctac
     6121 aactgtaggt gcagcagctt atcaatttag aattaaacgt taatagtttg gttttatact
     6181 atcataaaaa tagggattga attttcaatc cctattttgt tatttttatt ttgtgcaatt
     6241 aattcagcaa tgtttgatta tgtaataaat cttcttcttc tttcaatttc agctcccaat
     6301 cccaagcagt tttcaacatt gcatttaagt cgtatttaat ttgccagttt aatttttgta
     6361 tagccaattc attattagca aaaattgcaa caacatctcc taaacgtcta gggccaattt
     6421 cataattaag ctttaagcca cttacttctt caaatgtacg aatggcttct aaaacagtaa
     6481 cgccacaacc tgttcctaaa ttaaaaatat cacaattagt tctgttcctt ttgtcaagta
     6541 agtattgtat tcctaaagtg tgagcatgtg caatatcgca aacatgtata taatctctta
     6601 tgcaagagcc atctcttgta tcataatcgt taccatgtac aaacattgta gaaattttac
     6661 caattgcagt ttgggtaata gcaggcacca aattcattgg tttgccaatt ggcaactctc
     6721 caatatatgt actagggtgt gcaccaactg gattaaaata ccttagtaaa atacttgaaa
     6781 tgttatttac tttagagaag tctttaatca tttgttctcc catttgcttg gtagcaccat
     6841 aagggctttc agcttctttc attggcgatt tttctgtaac tggaatatta tctggattgc
     6901 catatacagt acaagaagat gaaaaaacaa aatgaggtat attaaactcg ctaacgcatt
     6961 taagtaaatt aattaaccca accatgttat tttcaaaata taacaaaggc ttttcaacac
     7021 tttcgcctac cgctttgtaa gcagcaaaat gaataatgcc atcaatatgt tgattttctt
     7081 gaaaaacagc tctagtgtca ttaaaatctt tcaggtcaac cttatagttt ttaattcttt
     7141 ttcctgtaat tttttgaatt ccatctatgg caatattggt agaacgtgaa ttatcatcaa
     7201 tagaaataac atcaaaatta ttttctatca aatctacaat tgtatgagag ccaatataac
     7261 cacaaccacc agttactaaa atagtaggca ttttgtatta tttgtttttg gcaaaataaa
     7321 ctattatttt atacaaaaag ttttaaaata tagtaaaaac cggcagccat tagtgcagaa
     7381 ataggaatag ttaatatcca agcccataat aagtttacag ttacacccca acgaaccgca
     7441 ctaagccttt tagtagcacc aacacctata atagagccag aaatagtatg tgttgtagaa
     7501 acaggtattc ctaaatgttc agaaatataa agagttactg cacctgcagt ttcagcactt
     7561 acaccttcta agggggtaac tttagttatt ctggttccca ttgtttttac aattttccag
     7621 ccaccactca atgtgcctaa accaatagca aaaaaactac aaaatggcac ccaactgtat
     7681 ttaataacaa attcattaaa agaaagacta tggcctaagg tagattcgtg ataaataatt
     7741 gcagcaccaa taatgcccat taccttttgt gcatcattac caccatgccc taaactaaat
     7801 gctgcactag aagcaagctg tagcttttta aaccaacttt ctgctttata atgattactt
     7861 cgtttaaaaa tatgtacaat taaaatagta attaaaaatg ctataaacat accaattaaa
     7921 ggtgctataa aaataaacaa tactgttggt aacactttgg catagttaat tacatcaatt
     7981 ccgtttttgc taaaagcacc agcataagct aaagatgcac caataaaccc acctaataat
     8041 gtgtgagagg aggaagaagg aataccaaac caccaggtaa ttaaattcca ggttattgca
     8101 gccattaaac ctgcaaaaat cacttctaag gttatatagt tttcatgtac acttttggcg
     8161 atagtattac caattttaaa ttctcctatc aaatattttg acacaaaaaa agcagcaaaa
     8221 ttaaaaactg ctgcccataa cactgcctga aacggagtaa gtactttggt tgaaactatt
     8281 gtggctatag agtttgcagc atcatgaaag ccattaatat aatcaaaaat aaaagccaat
     8341 gcaattataa caattagtaa aactggaaaa tccatagtaa gtattttgtg cttattaatt
     8401 taacttcttc aacgaaatat taagagtatt taataattat actttcaata acattggctg
     8461 catcttcgca tttatctgtt gcagtttcta aagcctggta aatttctctt ttcttaatta
     8521 cttctttaaa atcattttcc tgattaaata acatttcaat actcatatca aaaacatcat
     8581 cagcttggtt ttctatacta tttactttta ccaatgcttc tgtcatttct ctaagatgtt
     8641 tcatatctct taacccttta actgctttac taatttctgt tgtgccttgt aaaacaagtt
     8701 cagctaattt ttgaattccg ctatcatttg gatttacttt gtaaaagcta atttttttag
     8761 aagaagcata aatataatct gcaatatcgt ctaatgcagt agctaaatag tgtatgtctt
     8821 ctctatcaaa tggggtgatg aaatttctac ctaactcagt aaaaattaaa tgggtggcat
     8881 catcattttt gt
//
LOCUS       DAOQPK010000078         8797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_4325_length_8797_cov_4.338824, whole genome shotgun sequence.
ACCESSION   DAOQPK010000078 DAOQPK010000000
VERSION     DAOQPK010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8797
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4325_length_8797_cov_4.338824"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..1243)
                     /locus_tag="PLU36_05185"
     CDS             complement(206..1243)
                     /locus_tag="PLU36_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMZ46177.1"
                     /translation="MQTNYEKIIIVGQGLCGTFLSMYLHLAGKEIVVYNLPKKNSSSI
                     VTSGVINPVTGRRIVRTWMIEEIMPFAVNTYQKVEQLLQTSIIKQCNILDFHPTPQMK
                     IAFDDRINEEPFLQKPSNQNQWNEYLNYHFGFGEINPCWQIDIQNLLQSWREYLLTNR
                     LLKEEYFSEEQINLHKNNLVIYCDGADNTTNSYFKLLPYALNKGEALIAEINDLPRKN
                     IYKQTCAIVPYKKDLWWIGSNYEWNFENDLPSNHFREDMEARLKSFLKIPYKIVEHIA
                     AIRPANIERRPFIGFHPIHQNIGILNGMGTKGCSLAPYFAHQFSNYIVQGTAIHSMAD
                     IKRFSGILSKN"
     gene            complement(1264..1860)
                     /locus_tag="PLU36_05190"
     CDS             complement(1264..1860)
                     /locus_tag="PLU36_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HMZ46178.1"
                     /translation="MNYLAHAYLSFNKEDILIGNMISDFIKGKRKFDYPLQIQKGIQL
                     HRNIDSFTDNHFATKEAKQIFKPVVGLYAGAFIDVVYDHFLAIDKTEFETESHLNNFT
                     QTTYKSLMHNYSLMPPIFQQMLLKMKAQNWLFNYQFLWGIEKSFEGVVHRAKYLQSST
                     LVYNLFIQNYSSLQNYYSLFFKDVKAFSFNEFSSLISK"
     gene            complement(1868..2803)
                     /locus_tag="PLU36_05195"
     CDS             complement(1868..2803)
                     /locus_tag="PLU36_05195"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMZ46179.1"
                     /translation="MKEFLVIVLLIICTSFKSLAQTNEKDISIKINNGVLDGTLTTTN
                     NHAVLAIIIAGSGPTDRDGNNIAGVSANTYKMLAAELQKNNIATIRYDKRGVGKSLQK
                     NSSEDSLRFEDYINDAITFYHYAIDSLGYKKIFFIGHSEGSLIAIVAAQQVNVMGVVS
                     ISGAGRPIDEVILSQLESKEEVVYQQARDIFTILKNGKTIHDVPKYLYALFRPSVQPY
                     MISWLKYTPADEIKKIKPPVLIINGTCDIQTKPSEAQILYMANTKNKIVLIKKMTHTL
                     KNASDDCEDIGMKTYTDKTLPINEELIINISKFIN"
     gene            3093..3539
                     /locus_tag="PLU36_05200"
     CDS             3093..3539
                     /locus_tag="PLU36_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46180.1"
                     /translation="MKKLLFIMLICTAFSFKLKAADDLKVSNIIIENFQQSFPSVENV
                     EWKISKDVVKASFNNNNEFVTAYYFFDGSYLGYSKPVALDKLPENAIRNFTKNYPFPL
                     YNLTEVIEVTSPNGEINYYLSLLESKNGKRRILRVSEMGGVYSFND"
     gene            complement(3605..5632)
                     /gene="ispG"
                     /locus_tag="PLU36_05205"
     CDS             complement(3605..5632)
                     /gene="ispG"
                     /locus_tag="PLU36_05205"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HMZ46181.1"
                     /translation="MQLYCNSLTNYKRLLTKEVKIGDLLLGNNHPIRLQTMTTTDTMN
                     TIATVEQTIRCIEAGAELVRITAPSIKDAENLLHIKNELHKRGFKIPLVADIHFTPKA
                     AEISARIVEKVRVNPGNYVDKKKFEQIEYTDAEYLEEIERIRERFTPLIKICREYGTA
                     MRIGTNHGSLSDRIMSRYGDTPMGMVESAMEFLRIARNESYHNILLSMKASNPQVMVQ
                     AYRLLIQQMETEFGECYPLHLGVTEAGDGEDGRVKSAAGIGTLLEDGIGDTIRVSLTE
                     DPEFEIPVCKDLVKRYSSNNQQLTTKVPAINKLSYNPLEYKRRESFEIENIGKTHVPI
                     VIADLSKVETISPNTLRSVGYSYNSRTDKWNISDLAADYIFIGNKTLNFELPGTLKVI
                     VYPATWSLTNNKAKYFPIFDAKAFIENNKNNSSTNFVMVDCFSDDTTINDFSFLEELA
                     KDNTVVLCLSSTNKNSMQSIRRMYIELQNRNIKNPVVNICDSNWQTNDEHLIHFATET
                     GALFLDGFGDGICLGLTNNSYQNAIINNQQTTGRNYSSNQSIEQFINNTAFSILQATR
                     TRISKTEYISCPSCGRTLFDLQETTAKIRNRTNHLKGVKIAIMGCIVNGPGEMADADF
                     GYVGSGPGKITLYKGKEVVKRNIDSNIAVDELINLLKENNAWIEPELNKEN"
     gene            5798..6520
                     /locus_tag="PLU36_05210"
     CDS             5798..6520
                     /locus_tag="PLU36_05210"
                     /inference="COORDINATES: protein motif:HMM:NF021541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2272 domain-containing protein"
                     /protein_id="HMZ46182.1"
                     /translation="MKLLVLVYMLVWCGTLVAQTERSDSLLNFKNNLLQIVQEEYCFW
                     NDPVNRVETQFNSVEHVKKYWSCLNFHPTTKQIQSTKWQDRNPWSSAFISWCMKAAGY
                     GNNFFYAPNHAKYIRKALQNRENNLTENPFWAYDITEEEAAYPKPGDLLCKNREGMKY
                     ILHSIKENSISHCDVVVETDKINGIVITIGGNVSDKVNKRWVFLDNNGFIDTNAAWLS
                     FDADGVGHSGSQKDFYSVIKVQ"
     gene            6589..7542
                     /locus_tag="PLU36_05215"
     CDS             6589..7542
                     /locus_tag="PLU36_05215"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HMZ46183.1"
                     /translation="MKVTEHIAQAKDTLLSFEILPPLKGKTIQTLFNHLDPLMEFNPS
                     WINVTYHRSETAFKKKQDGTFEKVEIRKRPGTVGICAAIMNHYKIDTVPHFICGGFNK
                     RESEDALIDLNFLGINNVLVLRGDAEKNEPSFLPEPSGHQYAIDLQKQVVNLNHGIYL
                     DDDIRDGGKTNFCIGTAGYPEKHFEAPNLEIDLQRLKNKVDAGADYIMTQMFFDNEKF
                     FSYVNQCRNMGVTVPIIPGLKPITSKKQLSVLPRIFHVDIPTELSTEIMKCRTDEDVE
                     TVGTEWLIHQSKALKKFGVPVLHYYTLGKPKVIWNVVKEVI"
     gene            7631..8035
                     /locus_tag="PLU36_05220"
     CDS             7631..8035
                     /locus_tag="PLU36_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46184.1"
                     /translation="MQKIIAYILFFIFSISTFTSKAVIDDCCAVNNAKHTMPSHCKKS
                     CCTKEKKSIPKHAHKCCQVIAPNQLNYAVSNTNNIVVEASVSSKKIHCITYSYSFQNL
                     FEKPNSNYPIFFKQHTLQYAPKHYIQYCSYLI"
     gene            8089..>8797
                     /locus_tag="PLU36_05225"
     CDS             8089..>8797
                     /locus_tag="PLU36_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46185.1"
                     /translation="MKTLLLYTICLLLSLNVNAQTHKTYSFKVKGSCEMCKERIEQAA
                     KTAGAININYNLATQVLSFSLDNEKLNVADIEKAVANIGHDTEHFSSPDKTYNELPDC
                     CHYTRGNQFNTSKNLLRGIILEENTSGKMMPIAHVNVHDLVNEFSVQTNAKGAFEIPF
                     QSATYLSINHVNFKKDSIEVNENEFLTITLSHKGKNSLDDVVVTVKRSRYMSSTIISN
                     QINLGTKELTKAACCNLS"
BASE COUNT         2984 a         1299 c         1263 g         3251 t
ORIGIN      
        1 taatattatc tgtaggaatg gctctagtaa actcgtagct taattttact ttagatgcga
       61 aaaaccccat taaggcagtt gtaataagta aagctattaa tagtaatagc ctatttttta
      121 aaataaactg cccaagttta taccacatac tttttttatt ataactgttt tgaaagttgc
      181 aaagaaaagt atttttatag aagtattaat ttttagataa gatgccagaa aatcttttaa
      241 tatctgccat agaatgaatt gctgttcctt gtacaatata atttgaaaat tgatgggcaa
      301 aatatggtgc taaagaacag ccttttgtac ccatgccatt taaaattcca atattttgat
      361 gaataggatg aaagccaatg aatggccttc tttcaatatt tgcaggcctt atagcagcta
      421 tatgttcaac aattttataa ggaattttaa gaaaactttt caaccttgct tccatatctt
      481 ctctaaagtg gttactgggt aaatcatttt caaaattcca ttcataattg gagccaatcc
      541 accataaatc ttttttgtat ggtacaattg cacaggtttg tttataaata ttttttcttg
      601 gcaaatcgtt tatttcagct attaaagctt cgcccttatt taatgcataa ggtaataatt
      661 taaagtaaga attggttgtg ttatcagctc catcgcaata aataactaaa ttatttttat
      721 gcaaattaat ttgctcttca gaaaaatatt cttcttttaa tagcctattt gtaagtaaat
      781 attctctcca actttgtaaa agattttgaa tatctatttg ccagcaaggg ttaatttctc
      841 caaaaccaaa atgatagttt aagtattcgt tccattgatt ttgattggat ggtttttgta
      901 aaaatggctc ctcatttatt ctatcatcaa aagcaatttt catttggggt gtaggatgaa
      961 agtctaaaat attacattgc ttaataattg aagtttgtaa taattgctcc actttttgat
     1021 aggtatttac tgcaaaaggc ataatttctt caatcatcca tgttcttaca attcttcttc
     1081 ctgttactgg gtttattact ccactagtta caatacttga ggagtttttt ttgggaagat
     1141 tatatactac tatttcttta ccagccaggt gtaaatacat agataaaaat gttccacata
     1201 aaccttgtcc tactataatt atcttttcgt aattagtttg catattttat ttttcttaaa
     1261 aatttattta cttattaaac ttgaaaattc attaaatgaa aatgctttta catctttaaa
     1321 aaataaggaa taataatttt gcaaactcga gtagttttga ataaataaat tatacaccaa
     1381 agtagaactt tgcaaatatt ttgcacgatg tactacacct tcaaaacttt tttcaatacc
     1441 ccataaaaat tggtagttaa acaaccagtt ttgtgctttc atttttaata acatttgctg
     1501 aaaaattggt ggcattaaac tatagttatg cattaaactt ttataggtag tttgagtaaa
     1561 attattcaaa tgactttctg tttcaaattc ggttttatct attgctaaaa aatgatcgta
     1621 cactacatct ataaatgcac cagcatataa gccaacaact ggcttgaaaa tttgtttggc
     1681 ttctttagtt gcaaaatggt tatctgtaaa actatctata ttacgatgca attgaatacc
     1741 tttttgtatt tgcaatgggt aatcaaattt tctcttacct tttataaaat cgctaatcat
     1801 attaccaatt aatatatctt ctttattaaa agataagtat gcatgtgcta aatagttcaa
     1861 ttaattatta gtttataaat tttgaaatgt ttattatcaa ttcttcatta attggtagcg
     1921 ttttatctgt ataagttttc attccaatat cttcacaatc atctgatgcg ttttttaaag
     1981 tgtgtgtcat tttttttatt agcacaattt tgtttttggt atttgccata tacaatattt
     2041 gtgcttctga tggttttgtt tgtatatcgc atgtgccatt gataattaat acaggaggtt
     2101 taattttttt tatttcatcg gcaggggtat attttaacca ggaaatcata tatggttgta
     2161 ccgatggccg aaacaatgca tataaatatt ttggtacatc atgtatggtt ttaccatttt
     2221 ttaaaatagt aaatatatct ctggcttgtt gatatactac ttcttctttt gattctaatt
     2281 ggcttaatat tacttcatca ataggcctac ctgcaccact aatagataca acacccatta
     2341 cattaacttg ttgtgcagct actattgcaa ttaaagagcc ttcgctatgg ccaatgaaga
     2401 aaattttttt atatcctaag ctgtcaatag cgtaatgata aaatgttatt gcatcattaa
     2461 tataatcttc aaaccttaaa ctatcttcac tactattttt ttgcagactt ttaccaacac
     2521 ctcttttatc gtatctaatt gttgctatgt tatttttttg tagttctgct gctaacattt
     2581 tataagtatt tgccgatact cctgcaatgt tgtttccatc tctatctgta gggccagaac
     2641 ctgcaattat aatggccaat actgcatggt tgtttgttgt tgttaaagta ccatccagta
     2701 ctccattatt tattttaata gaaatgtctt tttcatttgt ttgtgccaaa cttttaaagg
     2761 aggtgcatat tattagcaac actattacta aaaattcttt cattccgtta taaatatttt
     2821 aataaaagta ggttattaaa accaaaatta taatttaacc aaaccataac attaatttta
     2881 atttttttaa gaaatgtgtt gaaactgctt tctgtattga gttttgttga attaatgaaa
     2941 atataaattc gttattggat taccgttcat catgtgtttt ttaccattgg ttggttttat
     3001 gtataactac ttaatcttgc tattaaacca atttattttt acatcatcaa ataaattgaa
     3061 aactaattaa atcatcttta aaactaaata aaatgaaaaa gttactattt ataatgctta
     3121 tttgcaccgc attctctttt aaattaaaag cagcagatga ccttaaagtt tctaatatta
     3181 taattgaaaa ttttcaacag tcatttccat ctgtagaaaa tgtagaatgg aaaattagta
     3241 aagatgttgt aaaagcaagt tttaacaaca ataatgaatt tgttactgcc tattattttt
     3301 ttgatggtag ttatttaggt tattcaaaac ctgttgcatt agataaattg ccagaaaatg
     3361 ctattaggaa ttttactaaa aattatcctt ttcctttata caacttaaca gaggtaatag
     3421 aagtaacaag cccaaatggt gaaataaatt attacttatc tttattagaa tctaaaaatg
     3481 gtaagagaag aattttaaga gtatctgaaa tggggggtgt ttactcattt aatgattaat
     3541 ctttcatcaa cttattttat tgaagttgca acattggata tttttcaatg ttgttttttt
     3601 ataattaatt ttctttattt agttctggct ctatccaagc attattttct tttaataaat
     3661 taattaattc atctactgca atattgctgt caatatttct ttttaccact tctttccctt
     3721 tgtacaaagt aatttttcca ggtccgctgc caacataacc aaaatctgca tcagccattt
     3781 cacctggtcc attaactatg cagcccatga ttgcaatttt tactcctttt aaatggttgg
     3841 ttctatttct aatttttgca gttgtttctt gcaaatcaaa taaagttctg ccacagcttg
     3901 gacaactaat atattctgtt ttagaaattc ttgtacgagt tgcttgcaaa atagagaatg
     3961 cagtgttatt tataaactgt tctatagatt ggttgctact ataatttctg ccagttgttt
     4021 gctggttatt aatgatagca ttttgataac tattatttgt taatcctaaa caaataccat
     4081 cgccaaaacc atctaaaaat aaggcacctg tttcggtagc aaaatgaatt aaatgttcgt
     4141 catttgtttg ccaattgcta tcgcaaatat ttacaactgg gttttttata tttctgtttt
     4201 gtaattctat atacattcta cgtatagatt gcatgctgtt tttattggta gatgaaaggc
     4261 ataaaacaac tgtattatct ttagccaatt cttctaaaaa agaaaaatca ttaatagtgg
     4321 tatcatcgct aaagcaatct accattacaa agtttgtaga ggagttgttt ttattatttt
     4381 ctatgaatgc ttttgcatca aatattggaa aatattttgc tttattattt gtaaggctcc
     4441 aagtagcagg gtaaacaatt acttttaaag ttcctggtag ttcaaagttt aaagttttat
     4501 ttccaataaa aatataatca gcagcaaggt cgctaatatt ccatttgtct gttctagaat
     4561 tatagctata accaacacta cgtaaagtat ttggtgaaat agtttctact ttgcttaaat
     4621 cagcaattac aataggcaca tgtgttttgc caatattttc tatttcaaat gattctcttc
     4681 ttttatattc tagagggtta taagataatt tatttatagc aggaactttg gtagttaact
     4741 gttggttatt tgaactgtat ctttttacta aatctttaca aacaggaatt tcaaattcag
     4801 gatcttcagt taaagaaacc ctgatggtat ctccaatacc atcttccaat aaagttccaa
     4861 tacctgctgc acttttcact cttccatctt caccgtcacc tgcttctgtt acacctaaat
     4921 gcagtggata acattcacca aactcagttt ccatttgttg tataagtaac ctataagcct
     4981 gtaccattac ttgtgggtta cttgctttca tgcttagtaa aatattatgg taagattcgt
     5041 ttcgagcaat tcttaaaaac tccattgcac tttcaaccat acccattggt gtatctccat
     5101 atcggctcat aattctatca cttaatgaac catgattagt accaattctc attgcagtac
     5161 catactctct acaaatttta attagaggag taaatcgttc tcttattctc tctatttcct
     5221 ccaaatattc tgcatcagta tattcaattt gttcaaattt ttttttatct acataattgc
     5281 cagggtttac tctaactttt tcaactattc tggcagatat ttcagcagct ttgggtgtaa
     5341 aatgtatatc tgccactaaa ggtattttaa aaccacgttt atgcaattca tttttaatgt
     5401 gtagtaaatt ttctgcatct tttatacttg gagcagtaat tcttacaagt tctgcacctg
     5461 cttcaataca acgaatagtt tgctcaactg tagcaatagt attcatggta tcggttgtag
     5521 tcattgtttg aagcctaata ggatgattgt ttcctaatag caaatcgcca attttaactt
     5581 ctttggttaa taaacgttta tagttggtta aagaattaca atatagttgc atagcataat
     5641 aataaagatg caaagttgct taaaaaatac ctattttaat taataaatta attgtgcaaa
     5701 aattagatca taatttataa attatataag ataccaacta atgggtattt ttaaaatgga
     5761 tgcttgtaaa taagtatttg cagcatatat tagctaaatg aaattgctag ttttagtata
     5821 tatgctggta tggtgtggta cacttgttgc tcaaacagaa cgttcggata gtttactcaa
     5881 ttttaaaaac aatcttttgc aaatagtgca agaagaatat tgtttttgga acgatcctgt
     5941 aaatagagtt gaaacacagt ttaattcggt tgaacatgtt aaaaaatatt ggagttgttt
     6001 aaattttcat cctacaacca agcaaattca aagtacaaaa tggcaagata gaaatccttg
     6061 gagttctgca tttatttcat ggtgtatgaa ggctgcaggc tatggcaata atttttttta
     6121 tgctcctaac catgcaaaat atataagaaa agctttacag aatagagaga ataacttaac
     6181 cgaaaatcca ttttgggctt atgatattac cgaagaagaa gctgcttacc caaagccagg
     6241 agatttactt tgcaagaata gagaaggaat gaagtatatc ttacattcaa tcaaggaaaa
     6301 ttctatctca cattgtgatg tagtggttga aacagataag attaatggta ttgtaattac
     6361 tataggaggc aatgtttcgg ataaggtaaa caaacgctgg gtttttttag ataataatgg
     6421 atttattgat actaatgcag catggttaag ttttgatgca gatggggtag gccattctgg
     6481 tagccaaaaa gatttttatt cggtaattaa agtacaatag ttttttgtaa caatacactt
     6541 tttaaagtac ccaatactta ttatgtttgc agctatattt tttatttaat gaaagttaca
     6601 gagcatattg cacaagcaaa agatacctta ctgtcttttg aaatattacc tcctttaaaa
     6661 ggaaaaacaa ttcaaacctt attcaatcat ttagacccat taatggagtt taacccaagt
     6721 tggataaatg taacttatca tagaagtgaa acagctttta agaaaaaaca agatggcact
     6781 tttgaaaaag ttgaaataag aaaaaggcca ggtacagtag gtatttgtgc tgctataatg
     6841 aaccattata aaattgatac agtaccacat tttatttgtg gaggttttaa taaaagagaa
     6901 agtgaagatg ctttaattga ccttaacttt ttaggcatta acaatgtatt ggtattaaga
     6961 ggagatgctg aaaaaaatga acctagtttt ttaccagaac caagtggcca ccaatatgcc
     7021 attgacttac aaaaacaggt tgtaaactta aaccatggta tttatttaga tgatgatatt
     7081 agagatggtg gaaaaactaa tttttgcatt ggcaccgcag gatatccaga aaagcatttt
     7141 gaagctccaa atctagaaat tgacttacaa aggttaaaaa ataaagtaga tgcaggtgca
     7201 gattatatta tgacacaaat gttttttgat aacgaaaagt tttttagtta tgtaaatcaa
     7261 tgccgaaata tgggtgttac tgtaccaatt ataccagggt taaaacctat tacatccaaa
     7321 aaacaattaa gtgtattgcc acgtattttt catgttgata ttccaactga actatcaaca
     7381 gaaattatga aatgtagaac tgatgaagat gtagaaactg ttggaacgga atggttaatt
     7441 caccaaagta aggcattaaa aaaatttggt gtacctgtat tacattacta cacattagga
     7501 aagccaaaag tgatatggaa tgttgttaaa gaagtaattt aaacatattt taatttttga
     7561 atctatctat taatctcttt tttgttatag attaacattg taatttctca caaatgccta
     7621 attttgcact atgcaaaaaa ttattgcata catattattt ttcattttta gtattagcac
     7681 ttttaccagt aaagctgtaa tagacgattg ttgtgctgtt aataatgcta aacatacaat
     7741 gcctagccat tgtaaaaaat cctgctgtac aaaagaaaaa aaatcaatac ctaaacatgc
     7801 acataaatgt tgccaggtta ttgctcccaa tcaattaaac tatgctgtta gtaataccaa
     7861 taatattgtt gtagaagctt ctgtttcttc caaaaaaatt cattgtatta cctattctta
     7921 cagttttcaa aatttatttg aaaaaccaaa ctcaaactat ccaatctttt ttaagcagca
     7981 tactttacag tatgccccaa aacattacat acagtattgt agttatttaa tatagatagc
     8041 ttccactttc tttttttaaa ttatttttta aattttaaaa ttcattaagt gaaaacttta
     8101 ttattatata ctatatgctt attactaagc ttaaacgtaa atgcacaaac acataaaacc
     8161 tattctttta aagtaaaagg tagctgcgaa atgtgtaaag aaagaattga acaagcagca
     8221 aaaacagcag gggcaattaa cattaactat aatttagcca cacaggtttt aagttttagt
     8281 ttagataatg aaaaattaaa tgttgcagat attgaaaaag cagtagctaa tattggccac
     8341 gatacagaac atttttcctc tccagataaa acgtataacg aattaccaga ttgttgtcat
     8401 tataccagag gtaatcagtt taatacttcc aaaaacttat taagaggtat tattttagaa
     8461 gaaaatacca gtggtaaaat gatgccaatt gcacatgtaa atgtgcatga tttggttaat
     8521 gaattttcgg tacaaaccaa tgcaaaagga gctttcgaaa ttcctttcca atctgccaca
     8581 tatttatcta taaaccatgt aaactttaag aaagatagta ttgaagttaa tgagaacgaa
     8641 tttttaacta taaccttaag ccataaaggc aaaaacagtt tagatgatgt ggtagttact
     8701 gtaaaaagaa gcagatatat gtcttctaca attatcagca atcaaataaa cttaggtaca
     8761 aaagaattaa ccaaagctgc ttgttgtaac ctatctg
//
LOCUS       DAOQPK010000079         8774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_4345_length_8774_cov_4.950682, whole genome shotgun sequence.
ACCESSION   DAOQPK010000079 DAOQPK010000000
VERSION     DAOQPK010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8774
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4345_length_8774_cov_4.950682"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PLU36_05230"
     CDS             complement(<1..597)
                     /locus_tag="PLU36_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding module family 20
                     domain-containing protein"
                     /protein_id="HMZ46186.1"
                     /translation="MANLKETTSSKNKKLKISFWLRYKTNYGENIFITFLNNDYKLTQ
                     PIAMHYYDEVFWTTDLFLSINELNDENIYYQYHVINEDGIIKQEAFADKYFNLHIGKR
                     EINIIDYWNGTELAENIFFTKPFLQTLIPKTTIKTYSPKGKNYKHIFKVKAPLLHPNK
                     TICLLGNVKELNNWNTQKPILLTKKEKEFYYSTFIKLPT"
     gene            693..1937
                     /locus_tag="PLU36_05235"
     CDS             693..1937
                     /locus_tag="PLU36_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ46187.1"
                     /translation="MLKLLNILWNSFLMAIQELKVHKLRTLLSLFGIAIGIFCIIGVL
                     ATINSLEQYIKNNIESLGRNTIYIQKSPWVNDENTPWWKYQKRPEITSFEQKELKKRA
                     KLISNVAYAMFYNSSISYGNTEISRVLWSGVTEEFDKILVLEVLHGRYLSNAEFERGT
                     NSVVLGYTIAKDLFGNPEYAIGKTISLGNKKAIVIGTLKEQGSSMDGWQFDDRIIVPH
                     LYCKQVANEENSGGFIIAKANDNYALDDVKGELTGILRAIRKIQPTEEDNFALNDISA
                     TADPLKSFFSQVTIGGWAIAGLSLIVGAFGVANIMFVTVRERTSQIGLKKAIGAKSKI
                     ILTEFLIESAFLCMIGGLIGLAFVWLLTKALGGVLPFPIFIAPNIILLAFNICVVLGV
                     LSGIIPATIAAKMNPVVAIRTK"
     gene            1967..2980
                     /gene="tsaD"
                     /locus_tag="PLU36_05240"
     CDS             1967..2980
                     /gene="tsaD"
                     /locus_tag="PLU36_05240"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HMZ46188.1"
                     /translation="MSIYILAIESSCDDTSASVCKDGEILSNYIANQAIHQQYGGVVP
                     ELASRAHLQNIVPVVDIAIKEAAKKIKTNFNLNQLNAIAFTQAPGLIGSLLVGTQFAK
                     SLALALNIPLIAVHHMQAHVLANLIEEEKPSFPFLCLTVSGGHTQIVIAHSALHLEVI
                     GETIDDAAGEAFDKTAKLLGLPYPGGPLIDKYAQLGNANAFKFAEPNIPDLNFSFSGL
                     KTSVLYFLQKQDKQFIEDNLYNICASVQQTIINILMKKLKKAVQQTNIKQICLAGGVS
                     ANSGLRNALIKTGQQNNWKTFIPKFEYCTDNAAMIAITGYYKFINQQFTELDVSPTAK
                     ASW"
     gene            3121..3474
                     /gene="rpsF"
                     /locus_tag="PLU36_05245"
     CDS             3121..3474
                     /gene="rpsF"
                     /locus_tag="PLU36_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HMZ46189.1"
                     /translation="MNNYELMVIFTPVLSEEEFKNAQKKYAEMVTENGGTIVHTNAWG
                     LKSLAYPIQKKTTGIYWVLEYKVDASFNEKLKVNLLRDEQVMRHLITKLDKYAVEYNA
                     VKKSGVGFNNQKVEA"
     gene            3476..3739
                     /gene="rpsR"
                     /locus_tag="PLU36_05250"
     CDS             3476..3739
                     /gene="rpsR"
                     /locus_tag="PLU36_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HMZ46190.1"
                     /translation="MAKNEIKYLTAIKQEKPKKKYCRFKKYGIKYVDYKDIEFLKRFI
                     NEQGKMLPRRLSGNSLKYQRKVSDAVKKARQMALLPYVTDLLK"
     gene            3838..4287
                     /gene="rplI"
                     /locus_tag="PLU36_05255"
     CDS             3838..4287
                     /gene="rplI"
                     /locus_tag="PLU36_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HMZ46191.1"
                     /translation="MEVILIQDVDNLGGKNELVKVKNGYARNFLIPQKFAVEANSSNL
                     KQLNERLKVAAKKEAALLAEITKVVETLKASPVKITAKTGTSGKIFGSVTSLQIARAI
                     REQKGYEIDRKRISINDEIKELGTYKASFDFGNSNTAEIDFEVVSEA"
     gene            4510..5649
                     /locus_tag="PLU36_05260"
     CDS             4510..5649
                     /locus_tag="PLU36_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMZ46192.1"
                     /translation="MNFTTSELTQQVAQTAKDFALQHIKPHLMEWDESQEFPIHIFKE
                     LGKLGMMGVLVPEQYGGAGLSYFEYNAIIQEISKVCGSIGLSVAAHNSLCTNHILTFG
                     TEEQKQKYLPKLANAEFIGAWGLTEPNTGSDAGNMKTVAVQDGNDWILNGTKSWITHG
                     KSGDVTVVICRTGEPRTSGNSTAFIVERGTKGFMAGKKENKLGMRASETAEMIFDNCR
                     ISDAQRLGNVGDGFRQAMKILDGGRISIAALSIGIAKGAYEAALQYSKERQQFDQPIA
                     NFQGISFKLADMATEIMASDLLIWQSCHLKETGQKLTKQSAMAKYYASEVAVKAATEA
                     IQIFGGYGYTKDFPVEKHYRDAKLCTIGEGTSEIQKLVIAREVLQ"
     gene            5738..6766
                     /locus_tag="PLU36_05265"
     CDS             5738..6766
                     /locus_tag="PLU36_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46193.1"
                     /translation="MKYQIGDEIIVLLTQEEGRVIDIISEDMVMIEVKGVRFPAYMNQ
                     IDFPYFYRFSKKKSTQETNKVTKIYIDNIPKEKPQPNKFKVEDGVWLSLIPIFTLDEF
                     NDEVVEKLKIYIINKTNVAYKFQYTLKLASHDNFALENEIIAHHDFYIHDLDFANVND
                     YPNFYIDFSLSKEEKLKAEHFETCIKLKPKQIFKRIEALKETNESNISYKLFEEYPDR
                     YFEENTILLDKLKDKGFRVYEASKVKENLQPARSVIDLHIEKLSDSWKHLSNFEILTL
                     QLNEFEKWYNIAIANKLPSFIVIHGVGKGKLKDEIHEILKSKKEVKNYINQYDARFGY
                     GATEIFFK"
     gene            6781..7130
                     /gene="rnpB"
                     /locus_tag="PLU36_05270"
     ncRNA           6781..7130
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLU36_05270"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(7218..7454)
                     /locus_tag="PLU36_05275"
     CDS             complement(7218..7454)
                     /locus_tag="PLU36_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46194.1"
                     /translation="MQKFKQEELIQYLYGESSADLTFEINEALKIDWELQEEINVLQR
                     TFKQLSNLKLASPRKSNVNAVLRYAKISEPVIDL"
     gene            complement(7553..7840)
                     /locus_tag="PLU36_05280"
     CDS             complement(7553..7840)
                     /locus_tag="PLU36_05280"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HMZ46195.1"
                     /translation="MKQLPSYITNKYLLSILFFIAWMLFFDQKDFFSTIEKRRELRTL
                     IDKNKYYFKEIEVSKKQLADFQSNSIAIEKFAREKYMLKKDNEDIYIIDEK"
     gene            complement(7924..>8774)
                     /locus_tag="PLU36_05285"
     CDS             complement(7924..>8774)
                     /locus_tag="PLU36_05285"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HMZ46196.1"
                     /translation="MNILNGGAHADNKIDFQEFMIMPVGAANFSEGLRWGTEIFHTLK
                     SVLKKKGYSTNVGDEGGFAPNIQSNEEAIETVLESINAAGYKAGSQIVIAMDAANSEL
                     WDAKKKKYVFHKSNGKILSSDDLVKFWETWVKKYPIVSIEDGMAEDDWKGWAALTQAI
                     GNKCQLVGDDLFVTNVERLKQGIDKNIANGLLVKVNQIGTLTETINAVTMAQYAGYNT
                     IMSHRSGETEDVTIADLAVALNCGQIKTGSASRTDRIAKYNQLIRIEEALAHTAIYPK
                     NLKFGK"
BASE COUNT         3207 a         1258 c         1402 g         2907 t
ORIGIN      
        1 tgttggtagt ttaataaaag ttgaataata aaattccttt tcttttttag ttaaaagtat
       61 gggcttttgt gtattccaat tattcaattc ttttacattt cccagtaagc aaattgtttt
      121 attggggtgc aataatggtg cttttacttt aaaaatatgc ttgtaatttt tgcctttggg
      181 tgaatacgtt tttatagttg tttttggaat aagagtttgt agaaatggtt tagtaaaaaa
      241 aatattttct gcaagttcag taccattcca gtaatcaata atattaattt cccttttacc
      301 aatatgtaga ttaaaatatt tatcagcaaa agcttcttgt tttattattc catcttcatt
      361 aattacatgg tattgataat aaatgttttc atcatttagt tcattaatac ttaaaaacaa
      421 atcagtagtc caaaaaacct catcataata atgcattgca ataggctgtg ttagcttgta
      481 atcattattt aaaaaagtta taaaaatatt ttcgccataa ttagttttat acctaagcca
      541 aaaactaatt ttaagttttt tattttttga tgatgttgtt tctttcaagt ttgccattaa
      601 attgatattg caaagtaacc agttaaataa aacctcacac actaatcata aactttactt
      661 agaaatacaa attaccttta attttgattt taatgttaaa actgctaaat attttatgga
      721 atagtttttt aatggctatt caagaactaa aggttcataa gttacgaaca cttttatcgt
      781 tatttggaat tgcaatagga attttttgca ttataggtgt gttagctacc ataaatagct
      841 tagaacaata tataaaaaat aatattgaaa gtttaggaag aaatacaatt tacattcaaa
      901 aatcgccttg ggtaaatgat gaaaatactc cttggtggaa atatcaaaaa agaccagaga
      961 taacatcttt tgaacaaaaa gaattaaaaa aaagagctaa attaatatct aatgttgctt
     1021 atgcaatgtt ttataactct agcattagtt atggcaacac agaaatctct cgtgtattat
     1081 ggtctggcgt tactgaagag tttgataaaa ttttagtgct agaagttttg catggcaggt
     1141 atttaagcaa tgcagaattt gaacgtggta ccaactctgt tgtattaggt tataccattg
     1201 ctaaagattt atttggtaat ccggagtatg caataggtaa aacaatttct ttaggcaata
     1261 aaaaagcaat tgtaattggt actttaaaag aacaaggcag ttctatggat ggttggcaat
     1321 ttgatgatag aattattgta ccacatttat attgtaagca agttgccaac gaagaaaact
     1381 ctggtggatt tattattgct aaagcaaacg acaattatgc cttagatgat gttaaaggtg
     1441 agttgacagg aattttaaga gctatcagaa aaatacaacc aaccgaggaa gataattttg
     1501 cattaaatga tatttcagca actgccgatc ccttaaaatc tttttttagc caagtaacta
     1561 ttggtggttg ggctattgct ggtttaagtt taattgttgg tgcatttgga gtagccaata
     1621 taatgtttgt aacggtaaga gaaagaacaa gccaaatagg attaaagaaa gcaataggtg
     1681 caaaaagcaa aataatactt acagaatttt taatagaaag tgctttttta tgtatgattg
     1741 gtggattaat aggtttggct tttgtttggc tgctaaccaa agcactagga ggtgttctcc
     1801 ccttccctat ttttattgct ccaaatataa tattattggc atttaatatt tgcgttgtat
     1861 taggtgtttt atctggtatt atacctgcta ctattgcagc taaaatgaat cctgtagttg
     1921 ctataagaac caaataaatt aatagttgga ttaaaaaata atactcttgt caatttatat
     1981 tctggccata gaatcttcct gcgatgatac ttctgcctct gtttgtaaag atggagaaat
     2041 attatcaaac tacattgcca accaagctat ccatcagcaa tacggaggtg tagtgcccga
     2101 attagcaagt agagcccatt tacaaaatat tgttcctgtt gtagatattg caattaaaga
     2161 agcagccaaa aaaattaaaa ctaacttcaa cctaaaccaa cttaatgcaa tagcttttac
     2221 acaagctcct ggtttaattg gtagcttatt ggttggtaca caatttgcaa aatctttagc
     2281 acttgcttta aacataccat taatagcagt acaccacatg caggctcatg tattagccaa
     2341 tttaattgaa gaagaaaaac ccagtttccc ttttttatgt ttaactgtta gtggtggtca
     2401 tacacaaatt gtaattgcac actcagcttt acatttagaa gttataggag aaactattga
     2461 tgatgcagcc ggagaggctt ttgataaaac agctaaatta ttaggcttac cttatccagg
     2521 aggtccacta attgataaat atgctcagct aggtaatgca aatgctttta agtttgccga
     2581 accaaatata cctgacttaa atttttcatt cagcggatta aaaacttccg ttttatattt
     2641 tcttcaaaag caggataaac aatttattga agataattta tacaatattt gtgcttctgt
     2701 tcagcaaacc attataaata ttttaatgaa aaagctaaaa aaagctgtgc agcaaaccaa
     2761 tataaaacaa atatgcttag caggtggagt aagtgctaat agcggtttaa gaaatgcttt
     2821 aataaaaaca gggcaacaaa ataactggaa aacatttatt ccaaaatttg aatattgtac
     2881 cgataatgct gcaatgattg ctattacagg ttactataag tttattaacc aacagtttac
     2941 tgaactagat gtaagcccaa cagctaaagc aagttggtaa aagttatttt aacttcaatt
     3001 tgtataaata aactaaaact cctacttttg cagccccaat tctgttttgg cagaatctat
     3061 tgtacaaact gtagaatagt ccaatgggtt aaaaccaatt attaaacaaa aaaaacagta
     3121 atgaacaatt acgaactaat ggtaattttc actcctgtac tttctgaaga ggaatttaaa
     3181 aatgctcaga aaaagtacgc tgaaatggtt accgaaaatg gtggtacaat tgtacacaca
     3241 aatgcatggg ggttaaagtc tttagcctat ccaattcaaa agaaaactac tggtatttat
     3301 tgggtgttag agtacaaagt agatgcttct ttcaacgaaa aattaaaagt taatttatta
     3361 cgcgatgagc aagtaatgag acatttaatt acaaaactcg ataaatacgc cgttgagtac
     3421 aatgcagtga aaaaaagtgg tgtaggtttt aacaatcaaa aagtggaggc ataatatggc
     3481 aaagaatgaa ataaaatacc ttactgccat taagcaggaa aaacctaaaa agaaatattg
     3541 ccgttttaaa aaatacggaa taaaatatgt tgactataaa gacatagagt ttttgaaaag
     3601 gtttattaat gaacaaggta aaatgttacc tcgtcgttta agtggtaact ctttaaaata
     3661 tcaacgtaaa gtaagtgatg cagtaaaaaa agctcgtcaa atggcattgt taccttatgt
     3721 aaccgattta ttgaaataaa ttacaaatac aattagtaac cattccctaa ttctttaaat
     3781 aaagatgaca gtctaaatca ataattgatt gggctctggg aactaaaaca aataaaaatg
     3841 gaagtaatat taatacaaga tgtagataat ttaggaggca aaaatgagct agtaaaagta
     3901 aaaaatggtt atgctcgtaa cttcctaata cctcaaaaat ttgctgttga ggctaattca
     3961 tctaatctta aacaattaaa cgaacgttta aaagtagctg caaaaaaaga agctgcttta
     4021 ttagccgaaa ttacaaaagt ggttgaaact cttaaagctt cgcctgtaaa aattactgct
     4081 aaaactggaa caagtggtaa aatatttggt agtgttactt ctttacaaat tgctagagca
     4141 attcgtgagc aaaaaggcta tgaaatcgat cgtaaaagaa tatcaattaa cgatgaaatt
     4201 aaagaattag gtacctacaa agcaagtttt gattttggta atagcaatac tgccgaaatt
     4261 gattttgagg ttgttagcga agcttaatta tataaaatat ctaccctata aaaatacctt
     4321 acaaattgta gggtattttt tatttagtta gtttatacca tataatcttt tcttcaataa
     4381 tttggctatt ttaaaataaa atatattttt gcaaagctaa caaacgttag taaggctttt
     4441 aacgattgat tctcattact agtcctgtag caatgcagga cttattttta attcaacatt
     4501 taactttata tgaattttac aacatctgaa cttacacaac aagtagctca aacagcaaaa
     4561 gattttgcac tacaacatat aaaaccccat ttaatggaat gggatgaaag tcaggaattt
     4621 cctatacata tttttaaaga attaggaaaa ttaggtatga tgggagtttt agtgccagaa
     4681 caatatggag gtgctggttt aagctacttt gaatacaatg ccattattca agaaatatcc
     4741 aaagtttgtg gctcaattgg cttaagtgtt gcagcacaca actctctttg tactaaccat
     4801 attttaactt ttggtacaga agagcaaaaa caaaaatatt tacccaaatt agcaaatgca
     4861 gagtttattg gtgcttgggg cttaacagag ccaaacacag gcagcgatgc aggaaatatg
     4921 aaaacagttg ctgtacaaga tggtaatgat tggattttga atggtactaa aagttggata
     4981 acacatggaa aaagtggcga tgttactgtt gttatttgta gaacaggtga accaagaaca
     5041 agtggtaata gtacagcatt tattgtagaa agaggaacaa aaggttttat ggccgggaaa
     5101 aaagaaaata agttaggaat gagagcaagt gaaactgctg aaatgatttt tgataattgt
     5161 agaattagcg atgcacaacg attaggaaat gttggtgatg gttttagaca agccatgaaa
     5221 attttagatg gaggaagaat ttctattgct gcactatcta taggtattgc taaaggagct
     5281 tatgaagcag ctttacaata ttcaaaagaa cgccaacaat tcgatcagcc cattgctaac
     5341 tttcaaggta ttagttttaa attggcagat atggctacag aaattatggc aagcgattta
     5401 ttaatttggc aaagttgcca tttaaaagag acaggtcaaa aactaaccaa acaaagtgca
     5461 atggcaaaat attatgccag cgaagtagca gtaaaagcag ctacagaagc catacaaatt
     5521 tttggaggct acggctatac aaaagatttt cctgtagaaa aacattatag agatgcaaaa
     5581 ctttgtacca taggagaagg aacttctgag atacaaaaat tagttattgc aagagaagta
     5641 ttgcaataat attataaata atgcaacttt ctaaagccga tattttcggc tttttttatt
     5701 tttatttttg acaactttaa aacaaccaga atataaaatg aaataccaaa taggtgatga
     5761 aataattgtt ttactcaccc aggaagaagg aagagtaata gatattataa gtgaagatat
     5821 ggtgatgata gaagtaaaag gtgttcggtt tcctgcatac atgaatcaaa tagattttcc
     5881 ttacttctat cgttttagca aaaaaaaatc aacccaagaa acaaataagg ttactaaaat
     5941 ttatatagat aatataccaa aagaaaaacc acaacccaac aaatttaaag ttgaagatgg
     6001 tgtgtggtta agtttaattc caatttttac tttagacgaa tttaatgatg aggttgtgga
     6061 gaaactaaaa atttacatca tcaacaaaac caatgttgct tataaattcc aatacacttt
     6121 aaaacttgct agtcatgata actttgcttt agaaaacgag ataattgccc atcacgattt
     6181 ttatatacat gatttagatt ttgccaatgt aaacgattat ccaaattttt atattgactt
     6241 tagcctaagc aaagaagaga agttaaaagc agagcatttt gaaacctgta taaagctaaa
     6301 acccaaacaa atttttaaaa gaatagaagc tttaaaagaa acaaacgaat ctaatatctc
     6361 ctataaactt tttgaagaat accctgatag gtattttgaa gaaaatacca ttttattgga
     6421 taaactaaaa gataaaggtt ttagggttta tgaagcaagt aaagtaaaag aaaatttaca
     6481 gcctgccaga agtgtaatag atttacatat tgaaaaactt agcgacagtt ggaagcattt
     6541 aagcaacttt gaaattttaa ccctacaatt aaatgagttt gaaaaatggt acaatattgc
     6601 cattgccaat aaactgcctt cttttattgt aattcatggt gttggtaaag gaaaactgaa
     6661 agatgaaatt catgaaatat taaaatcaaa gaaagaggta aaaaattata taaaccagta
     6721 tgatgctaga tttggttatg gtgctacaga aatatttttt aaatagtaca tttgcaaaag
     6781 ctataatctc gaactatcgt gttaaataaa attttaataa ggaaagtccg gacaacttag
     6841 agcaattcac cagttaatag ctggttatgt tttaaacata aagaaagtgc cacagaaaat
     6901 aactaccata tttttatatg gaaaaggtga aaatgtaagg taagagctta caatttattg
     6961 tagtaatata ataaatgggt aaaccttgaa tgttgcaatg ccacgtatag aaacgtttga
     7021 gagctgctcg ttcgagtttc agggtaggca gcaaagattg taacagtaat gttgcaacaa
     7081 gataaatgat agtttagaaa cagaatccgg cttatgagga ttatagcaat aaaaaacagg
     7141 cttaagcctg ttttttattt aaactaaata caacaaaaac tagttgtata attatttttt
     7201 tcaataaatc aaccaaatta aaggtctatt actggttcgc ttattttagc ataacgtaaa
     7261 acagcgttaa catttgactt acgtggagaa gctaatttta gattagataa ctgtttaaaa
     7321 gttctttgta aaacattaat ttcttcttgc aattcccagt ctatctttaa tgcttcattt
     7381 atttcaaatg ttaaatctgc tgaagattcg ccatacaaat attgtatcag ctcttcctgt
     7441 ttaaattttt gcataataat ttaagtagta tgtagttaaa aatctattaa agaaaacaac
     7501 actatataaa cgcatctctt taataaatat tgttataggg ctggttaaaa aactattttt
     7561 catcaattat atatatatcc tcattgtcct ttttgagcat atacttttct ctagcaaatt
     7621 tctcaattgc tattgaatta gattgaaaat cagcaagttg ctttttagaa acttctattt
     7681 ctttgaaata atatttattt ttatcaatta atgttcttaa ttctctcctc ttttctatgg
     7741 tggagaaaaa atctttttgg tcaaagaaaa gcatccaagc tataaaaaat aagatactta
     7801 ataagtattt attggtaata taagaaggta gctgcttcat aaaaaagttt gatgatgtaa
     7861 atataactat gtattaatta tactacaatc atcaaactat taacagatgt ttattaatat
     7921 atattattta ccaaatttta aatttttggg atatatagct gtgtgagcaa gggcttcttc
     7981 aatacgtatt aattggttgt attttgcaat tctatctgtt ctggatgcag aacctgtttt
     8041 aatttgacca cagtttaatg caactgccaa gtctgcaata gttacatctt ctgtttcgcc
     8101 acttctatga ctcataattg tattataacc tgcatattgg gccatcgtta ctgcattaat
     8161 ggtttctgtt aaagttccta tttggtttac ttttaccaac aatccattgg caatattttt
     8221 atctatccct tgttttaaac gttctacatt agtaacaaat aaatcatctc ctaccaattg
     8281 gcatttatta ccaattgctt gagttaatgc tgcccatcct ttccaatcat cttccgccat
     8341 accatcttct atagaaacaa ttggatattt ttttacccat gtttcccaaa atttcactaa
     8401 atcatcgcta cttaatattt tgccattgct tttatgaaaa acatattttt tctttttagc
     8461 atcccataac tcgctgttag cagcatccat tgcaattaca atttgagaac ctgctttata
     8521 tcctgcagca ttaatagatt ctaaaactgt ttcaattgcc tcttcgttgc tttgaatatt
     8581 aggtgcaaaa cctccctcat ctcctacgtt tgtactatac ccttttttct ttaatactga
     8641 ttttaaagta tgaaatattt ccgttcccca acgcaaacct tcgctaaaat ttgctgcacc
     8701 aacaggcata atcataaatt cctgaaaatc tattttatta tcggcatgag caccaccatt
     8761 taaaatattc atca
//
LOCUS       DAOQPK010000080         8705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_4396_length_8705_cov_4.623815, whole genome shotgun sequence.
ACCESSION   DAOQPK010000080 DAOQPK010000000
VERSION     DAOQPK010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8705
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4396_length_8705_cov_4.623815"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..1240
                     /gene="ribD"
                     /locus_tag="PLU36_05290"
     CDS             203..1240
                     /gene="ribD"
                     /locus_tag="PLU36_05290"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HMZ46197.1"
                     /translation="MSTDEKYMQRCLQLAKLGNGYVAPNPMVGAVLVYNHNIIGEGFH
                     EKYGSAHAEVNCINSVSEINKNKIPFSTLYVSLEPCNHFGKTPPCTSLIVKHKIKKVV
                     IGCTDTFNLVNGSGISFLKENGVEVVVNVFQHECETLNKNFFTFHQKKRPYIILKYAQ
                     STNNKIASHKSERTFISNYFSNNLVHRWRSEEAAILVGTTTALKDNPALTNRLGYGNN
                     PVRLVIDKHLQIPTHYNLLDNSTKTIIFNFLKDDVKQNTFFVKLNMHENVIHQIVQFC
                     TKNNLISILVEGGTNLLQSFINQNLWDEARVITNNTLFIEDGVDAPNFNGQILNKISL
                     LHDTITFYTPQ"
     gene            1243..1836
                     /locus_tag="PLU36_05295"
     CDS             1243..1836
                     /locus_tag="PLU36_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HMZ46198.1"
                     /translation="MKTDAEFYLTIAQESKAEYKDRGSRFLAFAFPVQDVEECKKQVA
                     TLKKIHTKAVHHCFAYKLGLDGNNFRANDDGEPAGSAGKPILGQINSKQLTNVLVVVV
                     RYFGGTLLGVPGLINAYKTATSLALQTTPIIQKPIEIIKDFQFDYTKMNEVMIIVKKY
                     NCTVLKQDAQLFCLLTVAIPKSSLEAVQFKINEIINR"
     gene            complement(1848..2348)
                     /locus_tag="PLU36_05300"
     CDS             complement(1848..2348)
                     /locus_tag="PLU36_05300"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HMZ46199.1"
                     /translation="MKKLILIILMIVSFQIVQAQSTFILIRHAEKDTSNKGSTTMNAN
                     PPLSQKGKERAKKLADVLNTYTINEIYSTNFIRTANTVTPLAIKNNIQIKFYNHKKLV
                     EFANQLLLEKNKTIVIAGHNTTTPALTNLLLKQNKFTALKETEYDKIFIVSINNMEAT
                     VKVIEY"
     gene            complement(2359..3549)
                     /locus_tag="PLU36_05305"
     CDS             complement(2359..3549)
                     /locus_tag="PLU36_05305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ46200.1"
                     /translation="MKYNTYIIKRKIESVFIYPFILLGKCIALLKPLKKEYNIFYFFP
                     FYHTGGAENVHALITQATGDSNCIIYFTKTSADTTFLKQFQQSNCDIKNISSFTDNKW
                     LYFLNLIYRGIIVTYINKQSKTPIVFNGQCNFAYKISPWVKKEIKLTELIHSFNSFSW
                     IRIPFIPYLHQTIMISKVRINDHIKQYQTIGIPQEYSNRINYISNGIELPENAPNKNY
                     NNHLNILYVGRGTIEKRVHIIAAIAEEVHKTNPEINFIFAGDVANAIPENLKQHCILK
                     GNIVDKNELTNLYRQAHILLITSSTEGFPLVVMEAMANGCTIIATPVGDIPIHIQNNQ
                     NGQVVTEVDNENKIIEEMKKFIIYYQKHKYLLEENSLRNIHYAQQNFSIHSFNKAYKK
                     ILSK"
     gene            complement(3546..4361)
                     /locus_tag="PLU36_05310"
     CDS             complement(3546..4361)
                     /locus_tag="PLU36_05310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ46201.1"
                     /translation="MLPLVTILLPAYNCEHFIKQTIDSVLNQTYQNFELLIINDGSTD
                     NTINIINSYTDHRLIHILNNENKGLIYSLNKGIQLAKGKYIARIDADDICLPTRIEKQ
                     VNWLEQNNNTALVATTICFINENNEHTGFWHLDKMCITEKEIKNAMKWECCIAHPSVM
                     MRAATIKMYSYSSKQKHTEDYDLWLQLLSDKLVIEKINEPLLLYRVHSNSVTSSIHRK
                     RNSFFTIATTKKRFLWSRIKHGKWGIFETQILYTFCINLIMGLGKEIKNKIGF"
     gene            complement(4425..6905)
                     /locus_tag="PLU36_05315"
     CDS             complement(4425..6905)
                     /locus_tag="PLU36_05315"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HMZ46202.1"
                     /translation="MKNINWKKILPHVYAIAIFLIVAIIYCKPALEDKVLHQADVTQW
                     KSMAQNQQAVLDATDKVPLWSNGMFGGMPGYMIKGWSNNMVGYYFMDLISLNIPKPFL
                     FFFLASICFYFLTQVLRANTWVGTIISLCYAYATYNVVIVAEGHDTKMLSLAVLPGLI
                     GAIQLIFNKKYLWGITLTALFSSVLVAQKHYQIAYYGILIVAFMSIAFVVQCILKKEL
                     KHLLFAGLFAIAGVALGALQNAVILLPDNEYTKESIRGGSQLANDHTKTTKEGLTKEY
                     AFSYSMYKTEPFVMLIPRMFGGSNKMELEPEKSKAVANLQAMNPQIAQQLQSALSFYW
                     GGIGGTSGPPYVGAIICFLALIGFVILNNKHKWWILALTVLTIMMSWGSFFEGFNNFL
                     FNHLPLYNKFRAPSMILVVPTILLNIMAVLTLQKIIETKDKAALFIQYKKGLMITGGV
                     FIILILMYFGFDYKGESDTNLITNINNIPDAETKAVYLDAGKKMLNGLVEDRKSLFMS
                     DILRALFYVAVAALAIWLLIKNKAKEWMLLASIGLFSFIDIMTVDVKYLNSDNYQYKE
                     DYETSFNPTPADKFILQDKSDYRVFDVSNGAQAAINYGARASYYHQSVGGYHPAKLSI
                     FQDLAENQLYNYPNCKPVLNMLNTKYIIHGTSKADQVEINRDACGPVWFIKALKIVGT
                     PNEEMNALTNLSVKDTAVIGKGFEQIANTKFSYDTTANITVEMKQNDVITYKSKANTE
                     QFAVFSEIYYNKGWNAYIDGKLTPYAKVNYVLRGMPIPAGEHEIMFKFEPKSHALGWK
                     FSGIAEMLIYALLALTLFSEWKNRNKKA"
     gene            6962..7243
                     /locus_tag="PLU36_05320"
     CDS             6962..7243
                     /locus_tag="PLU36_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46203.1"
                     /translation="MRVLLFFSRIALICNVCYVFSVIARYFSVEQLPAFLVSTLVVLG
                     FFAIFLNIMASFLWLVSVVVRKKIIPVSLGVVNLIFLIAQIFNSFYLQL"
     gene            7240..8037
                     /locus_tag="PLU36_05325"
     CDS             7240..8037
                     /locus_tag="PLU36_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HMZ46204.1"
                     /translation="MIQLIIKDKSTKLFIILSGFLIANALIAEVIGVKIFSLEETFGF
                     KPLAIKLLGEDLSLNLTAGVLLWPVVFIMTDIINEYYGVKGVRFLSYLSAFIIVYTFF
                     VFYASIHLSPANFFIISKQGSGVPNMQYAYEGVLGQGTKIIIGSITAFLLGQLIDAFT
                     FKKIKKITGEKKIWLRSTGSTLISQFIDSYVVLFIAFYIGSRIGAKNGDFVWSFQLFL
                     AVGTLNYIYKGLVAIILTPVIYLLHYFIEKYLGHEKATAMKEAAMNS"
     gene            8239..>8705
                     /locus_tag="PLU36_05330"
     CDS             8239..>8705
                     /locus_tag="PLU36_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HMZ46205.1"
                     /translation="MSKISINIATGSLQQAEIVVGIDLGTTNSLIAVIHPESKQAVAL
                     KEHDSSSLVPSVIYFDELNNAIVGDNAKQFLEKTPERTIFSSKRLMGKSYNDIKENEF
                     FFSYKVIDDDTDRLVKVQVGDKFYSPVELSSYILKELKARAEHILKTSVNKVV"
BASE COUNT         2942 a         1222 c         1304 g         3237 t
ORIGIN      
        1 tttgtgtttt ataataacct gtaatatgtt cgatagctag ctctgctata tttttagctt
       61 cactgatact ataaatggaa attaacttat tggttaattg tttaaatgct tgctcaacag
      121 tcataaaagc aaattaaaac tatttaaaat tatttataca taaagcttat agcttttaga
      181 ttatttttga actctttaga gaatgagtac tgatgaaaaa tatatgcaaa gatgtttgca
      241 gcttgcaaaa ttaggaaatg gatatgtagc tccaaaccca atggtaggtg cagttttagt
      301 atataatcac aatattattg gcgaagggtt tcatgaaaaa tatggcagtg cacatgcaga
      361 agtaaattgc attaacagtg tttctgaaat taataaaaat aaaattcctt tttcaacttt
      421 gtatgtttct ttagaacctt gtaaccattt tgggaaaacg ccaccttgta caagtttaat
      481 tgtaaaacat aaaataaaaa aagttgtaat tggttgtacc gatacgttca acttagtaaa
      541 tggttcgggt ataagctttt taaaagaaaa tggagtagaa gtagttgtaa atgtttttca
      601 gcatgagtgc gaaactttga acaaaaattt ttttacattt catcaaaaaa agcgtcctta
      661 tattatttta aagtatgctc aatctaccaa caataaaatt gctagtcata agagtgaaag
      721 aacttttatc agtaattact ttagtaacaa tttagtgcat cgttggagga gtgaagaagc
      781 tgcaatttta gtaggtacaa ctacagcttt aaaagataac cctgcattaa ccaataggct
      841 tggttatggc aataatcctg ttaggttggt aatagataaa cacttacaaa ttcctaccca
      901 ctataattta ttagataata gcacaaaaac cataattttt aattttttaa aagatgatgt
      961 aaagcaaaat accttttttg ttaaattgaa tatgcatgaa aatgttatcc atcaaattgt
     1021 acaattctgt actaaaaata acttaataag tattttggta gaaggtggca ctaatttgct
     1081 tcaaagtttc attaatcaaa acttatggga tgaagctagg gtaattacta ataatacttt
     1141 atttattgaa gatggagtgg atgctcctaa ttttaatgga caaattttga ataagattag
     1201 ccttctacat gatacaatta cattttatac tcctcaataa ctatgaaaac agatgctgaa
     1261 ttttatttaa ctattgctca ggaatctaaa gctgaatata aagatagagg aagtagattt
     1321 ttagcatttg cttttcctgt gcaagatgta gaagaatgta aaaaacaagt tgctacatta
     1381 aaaaaaatac atactaaagc ggttcaccat tgttttgctt ataagttggg tttagatggt
     1441 aataatttta gagctaatga cgatggcgaa ccagcaggct ctgcaggtaa acctatttta
     1501 ggacaaataa atagtaagca attaaccaat gttttagttg ttgttgtaag atattttgga
     1561 ggaacattat taggtgtacc aggtttaata aatgcttata aaactgcaac atcattggct
     1621 ttacaaacaa caccaattat tcaaaagcca atagaaatta ttaaagactt tcagtttgat
     1681 tatacaaaaa tgaatgaagt aatgataatt gttaaaaaat acaattgtac tgttttaaag
     1741 caagatgcac agttgttttg tttattaact gttgctatac ctaaatcaag cttagaggct
     1801 gtgcagttta aaattaatga aattattaat cggtaaaagt ttattcctta atattctatt
     1861 accttaactg tagcctccat attatttata ctaacaataa atattttatc gtactctgtt
     1921 tcctttaagg cagtaaactt attttgtttt aataacaggt tagttaatgc aggtgttgtg
     1981 gtattatgcc cagcaattac aatagtttta tttttttcaa gcaaaagctg attggcaaat
     2041 tcaactaact ttttatggtt gtaaaatttt atttgaatat tgttttttat agctaatgga
     2101 gttacagtgt tggctgtacg aataaaattt gttgaataaa tttcgtttat agtataagtg
     2161 tttaaaacat cagctaattt ctttgccctt tctttacctt tttgtgaaag tggagggtta
     2221 gcattcattg ttgtagagcc tttattactt gtatctttct ctgcatggcg tattaagata
     2281 aaagtagatt gtgcttgtac tatttgaaaa cttacaatca ttaaaattat tagaattagt
     2341 tttttcatac tggttagttt atttacttaa tattttttta tacgctttat taaatgaatg
     2401 aatactaaaa ttttgctgag cgtagtgaat atttcttaag gaattttctt ctaataaata
     2461 cttatgcttc tgataataaa taatgaattt tttcatttct tctattatct tattttcatt
     2521 gtcaacttct gttactactt gcccattttg attgttttga atgtgaattg gtatatctcc
     2581 tacaggtgtt gcaataatag tacaaccatt tgccattgct tccattacaa ccaaaggaaa
     2641 gccttctgta gaggaagtga ttaataaaat atgagcttgt ctatacaaat ttgtcaattc
     2701 atttttatca actatattac cttttaaaat acaatgttgc tttaagtttt ctggaattgc
     2761 attggcaaca tctcctgcaa aaataaaatt tatttctggg ttagttttat gaacttcttc
     2821 tgcaattgct gcaataatgt gtaccctttt ttctatggtg cctctgccaa catacaaaat
     2881 gtttaaatgg ttattatagt ttttattagg ggcgttttca ggaagttcaa taccgttgga
     2941 tatataatta attcggttgc tatactcttg tggaatacca attgtttggt attgttttat
     3001 atgatcattt attcttactt tacttatcat gatggtttga tgcaggtaag gtataaatgg
     3061 aattcttatc caggaaaaac tgttaaatga atgaattaac tctgttaact tgatttcttt
     3121 tttaacccaa ggagatattt tgtatgcaaa attgcattgc ccgttaaata caataggagt
     3181 tttactttgc ttatttatat aagtaacaat aatgccacga taaattaaat ttaaaaagta
     3241 caaccattta ttatcggtaa aagaggagat gttttttata tcacaattgc tttgttgaaa
     3301 ttgctttaaa aaagtagtat ctgcagaggt tttggtaaag taaattatac agttactatc
     3361 acctgttgct tgtgtaatta aagcatgaac attttctgca ccgccggtat gataaaaggg
     3421 gaaaaaataa aaaatattat attctttttt tagtggtttt aatagagcaa tacacttacc
     3481 taaaagtatg aaaggataga tgaatacact ctctattttt cgttttatta tataagtgtt
     3541 gtatttcaaa agccaatttt atttttaatt tctttaccta agcccattat taaattaatg
     3601 caaaaagtgt ataaaatttg tgtttcaaaa attccccatt ttccatgttt aattctactc
     3661 cataaaaatc tttttttagt agttgcaatg gtaaaaaaag aatttctttt tctatgaata
     3721 ctacttgtta ccgaattaga atgtacccta tacaatagta atggctcatt tattttttct
     3781 attactaatt tatcagataa aagctgtaac cacaaatcat aatcttcagt gtgtttttgc
     3841 ttgctgctat agctatacat tttaatggtt gcagctctca tcattacgct tggatgtgca
     3901 atacaacatt cccatttcat tgcattttta atttcttttt ctgtaataca cattttgtct
     3961 aaatgccaaa agccagtatg ctcattgttt tcatttataa agcaaatagt tgttgcaact
     4021 aatgcggtat tgttattttg ttctaaccaa ttaacttgct tttcaattct tgtaggcaaa
     4081 caaatatcat cagcatctat acgggcaatg tatttacctt ttgctagttg tatgccttta
     4141 ttgagagaat aaattaaacc tttattttca ttgttaagta tgtgtattaa tctatggtcg
     4201 gtatagctat taataatatt tattgtatta tctgttgaac catcattaat aattaataac
     4261 tcaaaatttt gataggtttg attaagtaca ctatcaatag tttgctttat aaaatgttca
     4321 caattatagg caggtaataa tatggtaact aatggcaaca attaaaattc tttaaaataa
     4381 aaagctatca gcgaaaaata ctgatagctt ttataaaaat atatttaagc ttttttattt
     4441 ctatttttcc attcggagaa aagtgttaaa gccaatagag cataaataag catttcggca
     4501 attccagaaa acttccaacc taaagcatga cttttaggct caaatttaaa cataatttca
     4561 tgctctcctg caggtattgg catgcctcgt aatacataat ttactttagc ataaggtgta
     4621 agtttaccat caatataagc attccaacct ttgttataat atatttcact aaacactgca
     4681 aattgctcag tgttggcttt gcttttataa gttattacat cattttgttt catttctaca
     4741 gtaatatttg ctgtagtatc gtaactaaat tttgtattgg ctatttgctc aaatcctttt
     4801 cctataactg cagtatcttt tacagataag ttggttaaag cattcatttc ttcattagga
     4861 gtaccaacaa tttttaatgc cttaataaac caaacaggtc cacaagcatc tctattaatt
     4921 tctacctgat cagctttgct tgtaccatgt ataatatact tggtatttag catgtttaaa
     4981 acaggtttac agttaggata attgtataat tgattttcgg ccaaatcttg aaaaatgctc
     5041 aatttggcag gatgatagcc tccaacagat tgatgataat aagatgctct tgcaccataa
     5101 tttattgcag cctgtgcacc attgcttaca tcaaaaactc tataatctga tttgtcttgt
     5161 aaaataaatt tatctgcagg tgtagggtta aaagaagttt cataatcttc tttatattgg
     5221 tagttatcgc tatttaaata tttaacatca actgtcataa tatcaataaa agaaaataat
     5281 ccaatagatg caagaagcat ccactcttta gctttatttt ttattaatag ccaaatagca
     5341 agtgcagcta cagcaacata aaataatgct ctaagaatgt cgctcataaa taaacttttt
     5401 ctgtcttcca ctaaaccatt caacattttt tttccagcat ctaaataaac agctttggtt
     5461 tctgcatcag gaatattgtt gatgttggtt attaagttgg tgtcagattc tcctttataa
     5521 tcaaagccaa aatacatcag tattaaaata ataaaaacac caccagtaat catcaaccct
     5581 tttttatatt gtataaaaag tgcagccttg tctttagttt caataatttt ttgaagggta
     5641 agtacagcca ttatatttaa taaaatggta ggcactacta aaatcatact aggtgctcta
     5701 aacttgttat acaaaggcaa atgattaaat aaaaagttgt tgaacccttc aaaaaagctt
     5761 ccccaactca tcataatagt taaaactgta agggctaata tccaccattt atgtttgttg
     5821 tttaaaataa caaaaccaat taatgcaaga aaacaaataa ttgcaccaac ataaggtggg
     5881 ccagaagtgc caccaattcc tccccaataa aaacttaaag cactttgtaa ttgttgagca
     5941 atttgcgggt tcattgcctg taaattagct actgctttag atttttctgg ttccaattcc
     6001 attttattac ttcctccaaa cattcttgga ataagcatta caaaaggctc tgttttgtac
     6061 atgctgtaac taaatgcata ttctttagtt aaaccttctt tagtagtttt ggtatggtca
     6121 ttggctaatt gcgaaccacc tctaatagat tctttggtat attcattatc tggcaataat
     6181 ataacagcat tttgtaatgc ccctaatgca acaccagcta tagcaaataa acctgcaaat
     6241 aataagtgtt tcaattcttt tttaagaata cattgaacca caaaagcaat actcataaaa
     6301 gcaacaatca aaataccata atatgcaatt tgatagtgtt tttgtgccac tagtacacta
     6361 gagaatagtg cagttaatgt tatgccccat aaatattttt tattaaatat aagttgaatg
     6421 gcacctatta atccaggtag tactgcaagc gatagcattt ttgtatcatg cccttctgca
     6481 acaataacta cattataagt tgcataagcg taacacaatg aaattatagt tcctacccaa
     6541 gtattagctc ttaatacttg ggttaaaaag taaaagcaaa tacttgctaa aaagaagaat
     6601 agaaaaggct ttggaatatt tagcgaaata agatccataa aatagtaacc aaccatatta
     6661 ttactccagc ctttaatcat ataacctggc ataccaccaa acataccatt tgaccaaagt
     6721 ggaaccttat cagtggcatc taaaactgct tgttgatttt gtgccataga tttccattgt
     6781 gtaacatctg cctgatgtaa aaccttatct tctaaggcag gtttacaata aataattgca
     6841 actattaaaa aaattgcaat ggcataaaca tggggtaaaa ttttcttcca atttatattc
     6901 ttcatttatt ttctttttat aacaagcaaa ttaatgttat ttttcagttc aaaataaaaa
     6961 tatgagggtg cttttatttt ttagtagaat tgctttaata tgtaatgtgt gttatgtatt
     7021 ctctgtaata gctagatatt ttagtgtaga acaactacct gcatttttag taagtacctt
     7081 ggttgttctt ggtttttttg ctattttttt aaatattatg gctagttttt tatggttggt
     7141 aagtgtggtt gtaagaaaaa aaataattcc agtaagcctt ggtgttgtta atttaatttt
     7201 tttaatagcc caaattttta attcatttta tcttcaatta tgattcagtt aattattaaa
     7261 gataaatcaa ctaagttgtt tattatacta agtggatttt taattgccaa tgccttaatt
     7321 gcagaagtaa ttggggttaa aattttttcg ttagaagaaa cttttgggtt taaaccatta
     7381 gctattaaat tgcttggaga ggatttaagc cttaatttaa ctgcaggtgt tttactttgg
     7441 ccagtagttt ttataatgac tgatattatt aatgaatact atggagtaaa aggtgtgcgg
     7501 tttttatctt acctaagtgc atttattatt gtatatactt tttttgtttt ttatgcatct
     7561 attcatttat ctccagccaa tttttttatt ataagtaagc aaggtagtgg cgtgcctaat
     7621 atgcaatatg cttatgaagg tgttttaggg caaggaacaa aaattataat tggtagcata
     7681 actgcatttt tattaggaca attaattgat gcgtttacat ttaaaaaaat aaaaaaaatt
     7741 acaggagaaa agaaaatttg gttaagatca acaggctcta ctttaatatc tcaatttata
     7801 gatagttatg tggtattgtt tattgctttt tatattggct ctagaatagg ggctaaaaac
     7861 ggcgattttg tttggagttt tcaacttttt ttagctgttg gtacattaaa ttatatttac
     7921 aaagggttgg tagcaattat acttacacct gtaatttatt tactacatta ttttattgaa
     7981 aaatatttag ggcatgaaaa agcaacagcc atgaaagaag ctgcaatgaa tagttaacaa
     8041 tttctaaata ttcattatca tgctttttag agtttgattt tttcagttac tattcttgat
     8101 actaattagt ttatagaatt aaactacaat tatagtagca ttgttttttc tttcttatct
     8161 cataaaccat ttcggttact ctacatttgc aatcattatt tttgctgaaa ttattaatcc
     8221 ttcagtttca gattagctat gtcaaaaatt tctatcaata ttgcaactgg tagcttacag
     8281 caggcagaaa ttgttgtagg catagattta ggtactacaa atagtttaat agctgttatc
     8341 catccagaga gcaagcaggc agttgcctta aaagagcatg atagcagtag ccttgtacca
     8401 agtgttattt attttgatga attaaataat gcaattgttg gagataatgc aaagcaattt
     8461 ttagaaaaaa ctcctgaacg aaccattttt tcttcgaaaa gattaatggg gaaaagctat
     8521 aatgatatta aagaaaatga gttttttttc tcctataaag taattgatga tgatactgat
     8581 agattagtga aagtgcaggt aggggataag ttttattcgc cagttgagct ttcctcttat
     8641 attttaaaag aattaaaagc aagagcagag catattttaa aaacttctgt aaacaaagtg
     8701 gtaat
//
LOCUS       DAOQPK010000081         8578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_4491_length_8578_cov_4.786695, whole genome shotgun sequence.
ACCESSION   DAOQPK010000081 DAOQPK010000000
VERSION     DAOQPK010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8578
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4491_length_8578_cov_4.786695"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            401..1153
                     /locus_tag="PLU36_05335"
     CDS             401..1153
                     /locus_tag="PLU36_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46206.1"
                     /translation="MKAKIFYSILLVSILTSCSSAYKTSQTPDDVYYSPAEGNYYVEK
                     EVKTEDEDYLRMKVRNNRFRTIDDYSYWNDVRLTYTNCYTNYPYNGVHNWNSYYYNNY
                     NSWNNHIYYNSPCIGSNWYSFNYNPWGSWNSPFYYMVPYKNPTVYTGNTKASYVTAYT
                     TKSYSNTNVKTGIKAGTSSTNRNTGFGKLVKRVFSTNGNTGSGNAGWDRPARTYTNSS
                     GTTTSGSSSAGGRSGGYKSTGSSSSSSRSGRN"
     gene            1214..2749
                     /locus_tag="PLU36_05340"
     CDS             1214..2749
                     /locus_tag="PLU36_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46207.1"
                     /translation="MKKIYSISLLIIVALKLNAQVPDDALRTAWYTFNGSARNMAIGG
                     VMGSLGGDITAANINPAGLGLFKTKEFILSPGFNLNNNNLLFRGTDTTDKKNSFNYGT
                     IGFVFGKPNNPNYSKFTSSAFSISVNQLANYNNSIQFKGFNNFSSFSEQYLEELIADG
                     ANMTAAEQNYIYGSSLAFRTYLIDTIADANGNLIGFQSLVPISTGVNQTYNEKTRGGL
                     HEIALGFASNLEDKLYIGGSVNIPILSFQRDFTYSENDATNNTNNDFDNFIYTEKFKS
                     FGAGFGAKLGFIYKPQERWRLGFAFHTPQFMFFKDKIRTWMTTNTEAYAGIKSESSDA
                     LNSGNAGERKYNLVTPWRALVSASYVFNEVKDVRKQRAFISADIEFVNYRGARFARSE
                     EDDYVAKAYYKTVNATVKDYYKGNFNFRVGGELKLNTFMFRLGAAYYGSPYKERVLNA
                     NKMMLTGGIGYRNYGFFIDLAYAHSFNKDVVFPYRLNDKPNTFAEQTGSRGNIMLTFG
                     VKL"
     gene            complement(2783..3412)
                     /gene="rsmG"
                     /locus_tag="PLU36_05345"
     CDS             complement(2783..3412)
                     /gene="rsmG"
                     /locus_tag="PLU36_05345"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HMZ46208.1"
                     /translation="MDIIKKYFDDFTPKQIEQFAALKNLYKEWNEKINVISRKDIDEI
                     YLHHVLHSLSIAAIGNFVPQTQVIDIGCGGGFPGVPLAIFYPDVHFHLVDSIGKKLKV
                     VNAICEAIEVKNITTQHTRVEEIKNKKFDFAISRAVAPLKELWHWSNPIIKKAVLPNQ
                     EMANGLICLKGGDLHQEIFESQTKPSMMGIYNIFKEEYFKEKFILYVKK"
     gene            complement(3432..4562)
                     /locus_tag="PLU36_05350"
     CDS             complement(3432..4562)
                     /locus_tag="PLU36_05350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ46209.1"
                     /translation="MIIDIPPIVFTIVFYIFCSVIAIQLFYYLFFFTRLAFYRKKNKA
                     NSVQHPISIIICARDEAHNLANNLPGILVQNYKTTHEVIVVNDNSVDETKYLLDEFKK
                     SFKNLNQIELSQEAKMIEGKKFPLSIGIKSAKYETVLLTDADCLPASEDWMYYMQDAY
                     NDDIEVVLGYGAFKKRKGILNKLIRFETFHTALQYFSYALAGIPYMGVGRNLSYKKDI
                     FFRNKGFSSINMIPSGDDDLFINKVTNKKNTAIVIDPLAHTLSEPKKTWDEWMTQKYR
                     HYTTAKFYKPIHKFLLGLYSFSLFCIYPLLIISAVFYCWWLTLSVFAIRFIVQAIIMF
                     KTMKKLNEKDLFPWFILLDLWMPLYYLFTIPAIWKSPKRTWG"
     gene            complement(4652..6244)
                     /locus_tag="PLU36_05355"
     CDS             complement(4652..6244)
                     /locus_tag="PLU36_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HMZ46210.1"
                     /translation="MQKEISLKITPSEAASNTTITEIIAKTVGVSPNEVFGFYKLKQS
                     IDARSRKQIWVNITVNAFINEKFIERNIEKINFQDVSNSNKKVIIVGAGPAGIFAALK
                     LIELGIKPIVVERGKDVRSRRRDLAILNKEGKINTESNYCFGEGGAGTYSDGKLYTRS
                     NKRGSIDRILNLFVQFGAEEKILYQAHPHIGTNKLPSIITAMREQIINCGGEVHFEKK
                     LTDLIIENETVKGIVSNKEEKIFANAVILATGHSARDIYELLYAKKIFIEAKPFALGV
                     RIEHPQNIIDTAQYHLTNYQKQKRSDFLPPATYNLVEQVNGKGVFSFCMCPGGIIAPA
                     ATNNNELVVNGWSPSKRDNPFANSGMVVSVELKDILDIKECRNQNNSPLALMNFQKAV
                     EEKAYTFGGGNFVAPAQRVVDFCTNKISSNLPKCSYLPGINSSDITAVLPAFIYTRLQ
                     KALQIFGQKMKGYYTNEAIIVATESRTSSPVRIPRDSKTLQHPQLKNLYPCSEGAGYA
                     GGIVSAAMDGEKVAENIALNWF"
     gene            6592..7155
                     /locus_tag="PLU36_05360"
     CDS             6592..7155
                     /locus_tag="PLU36_05360"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HMZ46211.1"
                     /translation="MTKTELRKIYKEKRKAISSKDKLKYDDLILLQLQKLYFENVQVL
                     LTYWPMELEPNMQIVTSYIKHTNYALRVAYPVTNFSNNTMCAIGVEDDTSFTTNTFGL
                     TEPKSGEEINANEIDIVFVPLFIFDLRGYRVGYGKGFYDKFLASCKNDVIKIGFCYFE
                     PIEKIDDTNNFDIPLNYCITPHNIYEF"
     gene            7145..8236
                     /gene="lpxK"
                     /locus_tag="PLU36_05365"
     CDS             7145..8236
                     /gene="lpxK"
                     /locus_tag="PLU36_05365"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HMZ46212.1"
                     /translation="MNFNTLFLKSFRVLLLPFAIIYGIVVSIRNWLFNKNYFKSAKFN
                     FPLICVGNLAVGGTGKSPMVEYLVKILQPHFKTATLSRGYKRKTKGYALANTQTTALE
                     IGDEPMQFHLKFPDVAVAVGEERLVAIPQLLHDKPDLEVIVLDDAFQHRSVNAGLNII
                     LTEYSNLYTQDFFLPTGDLRDEKRSAVRADIIVVTKCPTSITQDKKYKILRSIKPLKH
                     QRVFFTTIDYGTPYHIYNNQDEWVLTPNEEVLLVCGIANPKPLKEYLHTAVHTYYQKD
                     YSDHHIFNIDDLNEIKQVFDKIKANRKLILTTEKDAVRLMKFSEVLKDLPLYVLPVKH
                     KFLFDDTQVFHNRIIDFVNNFRLELDEEK"
     gene            8223..>8578
                     /locus_tag="PLU36_05370"
     CDS             8223..>8578
                     /locus_tag="PLU36_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HMZ46213.1"
                     /translation="MKKNNKRQHHTTKETHLPTNTLKGILEVTNSGIGYVIMPEGVGD
                     ILVRQDNFNTAFHGDTVRVKVIKENFKSGKREGKITEVVQRKQTEFIGKLQLSNAFAF
                     FIPDSNKPMPDFFIPK"
BASE COUNT         3032 a         1270 c         1299 g         2977 t
ORIGIN      
        1 attttatcca acacatctct catattttcc caaagtgcaa acccataagg tttaataacc
       61 atacaacccc ttacagcact ataatctgcc agacctcctt ttaaaataag gtcgttatac
      121 cattggctat aatcttgttg cctacttgta atttctttac tcataaaatt ataataatgt
      181 attttttttg ttagtcagct tttagttatt tatttaacta tcttggcgtc gcacccttca
      241 gggtttataa cttttatcct cattttaaaa attttaacat actaacttgt gttagtaaat
      301 tttacggcac cacatttgta ttaaatataa aagtaaataa atactaaacg gtggcaaatg
      361 tatgcaactt cgcattatac taaatttaaa actatttatt atgaaagcta aaattttcta
      421 ttctatactt ttggtaagta ttttaacttc ctgttcatct gcctataaaa caagccaaac
      481 acccgatgat gtttattatt caccagcaga ggggaactat tatgtagaaa aagaagttaa
      541 aacagaagat gaggattatt taagaatgaa agtgcgaaac aataggttta gaactattga
      601 tgattatagt tactggaacg atgttagact aacttatact aattgttaca caaattatcc
      661 atacaatggc gttcataatt ggaatagtta ttactataat aattataata gttggaataa
      721 ccatatatac tataattcac cttgcattgg cagcaattgg tatagcttta attacaatcc
      781 ttggggtagt tggaacagcc ctttttatta tatggttcca tacaaaaatc ctactgtata
      841 tacaggcaat acaaaagcaa gctatgttac tgcctataca acaaaaagct atagtaatac
      901 caatgtaaaa actggtatta aagcaggtac tagttctaca aacagaaata caggttttgg
      961 aaaattagtt aaaagagtat tttctacaaa tggtaatact ggtagtggca atgcaggatg
     1021 ggatagacca gcaagaactt atactaatag ttctggtaca acaacttctg gcagtagtag
     1081 tgcaggtggt agaagtggag gttacaaaag tacaggtagc agtagcagta gtagcagaag
     1141 tggtagaaat taaaatataa ctgctgcaag agtggttcaa cccaaatatt aaaaaacttt
     1201 tatttcattt ctaatgaaaa aaatatattc aataagttta ctcataattg ttgctttaaa
     1261 actaaatgca caagtgccag acgatgcact tagaacagca tggtacactt ttaatggaag
     1321 tgcaagaaac atggctattg gaggtgtaat gggcagttta ggaggagata ttactgcagc
     1381 taatattaac ccagcaggct taggcttatt taaaacaaaa gaatttattt tatcgcctgg
     1441 ttttaattta aacaataata atttattatt cagaggaaca gataccacag ataaaaaaaa
     1501 tagttttaat tatggtacta taggctttgt atttggcaag ccaaataacc ccaattacag
     1561 taagtttaca agtagtgcat tttccatatc agttaatcag ttagcaaact acaacaatag
     1621 tattcaattt aaaggcttta ataatttcag ttctttttca gaacaatatt tggaagaatt
     1681 aattgcagat ggtgctaaca tgactgctgc agaacaaaac tatatttatg gatcttcttt
     1741 agcatttaga acttatttga ttgatacaat tgcagatgca aatggtaact taattggatt
     1801 tcaaagtttg gtgccaatct ctactggagt taatcaaacg tataatgaaa aaactagagg
     1861 tggattacac gaaattgcgt tgggctttgc aagtaactta gaagataaat tatatattgg
     1921 tggtagtgtt aatattccca ttttatcttt tcaaagagat tttacttact ccgaaaatga
     1981 tgcaactaac aataccaata acgattttga taattttatt tataccgaaa aatttaaaag
     2041 ctttggtgca ggctttggtg ctaaacttgg ttttatatat aaaccacaag aacgctggcg
     2101 tttaggtttt gcatttcata cacctcagtt tatgtttttt aaagacaaaa ttagaacttg
     2161 gatgacaact aataccgaag cttatgcagg tattaaaagt gaaagcagcg atgcattaaa
     2221 tagtggcaat gcaggagaaa gaaagtataa tttagtaacc ccttggagag cattagtaag
     2281 tgcaagctat gtttttaatg aagtgaaaga tgtacgcaag caaagggcat ttataagtgc
     2341 agatattgaa tttgttaatt accgtggtgc tagatttgca agaagtgaag aagatgatta
     2401 cgtagctaag gcatattaca aaactgtaaa cgcaactgta aaagattatt acaaaggaaa
     2461 ttttaatttt agagtaggcg gcgagctaaa gttaaataca tttatgttta ggttaggagc
     2521 tgcttattat ggaagccctt ataaagaacg tgttttaaat gcaaataaaa tgatgttaac
     2581 tggtggaata gggtatagaa attatggctt ttttattgat ttagcttatg ctcatagctt
     2641 taataaagat gttgtttttc cttatcgttt aaacgataag cctaacacat ttgcagagca
     2701 aacaggaagt agaggaaata ttatgctaac ttttggagtt aaattgtaat aagttattta
     2761 ttgataaaaa tagaagcata gtttacttct taacatacaa aataaacttt tctttaaaat
     2821 attcttcttt aaaaatattg taaataccca tcatagaggg tttggtttgg ctttcaaaaa
     2881 tttcctgatg caagtcgcca ccttttaaac aaattaatcc gttagccatt tcttgatttg
     2941 gtaaaactgc ttttttaata atggggttac tccaatgcca caattctttt aatggagcaa
     3001 ctgcacggct aatagcaaaa tcaaattttt tgttttttat ttcttccact ctggtatgtt
     3061 gtgtagtaat attttttact tcaatagctt cgcaaattgc atttacaact ttcaattttt
     3121 taccaatact atccactaaa tgaaaatgaa catctggata aaaaattgct aaaggaacac
     3181 caggaaaacc accaccacag cctatatcaa taacttgggt ttgtggcaca aaattgccaa
     3241 ttgctgcaat acttaaagag tgtaatacat gatgcaaata aatttcatct atatcttttc
     3301 ttgaaataac atttattttt tcattccact ctttgtacaa attttttagt gcagcaaatt
     3361 gttcaatttg ttttggagta aaatcatcga aatatttttt tattatatcc attgtacaaa
     3421 atcaatagta attaacccca agttctttta gggcttttcc aaatggcagg aatagtgaat
     3481 aaataatata aaggcatcca caaatccaat aatataaacc aaggaaataa gtctttttcg
     3541 ttcaacttct tcattgtttt aaacataata attgcctgca ctataaacct tatagcaaat
     3601 acagatagag ttaaccacca acaataaaaa acagcactta ttattaataa tggataaata
     3661 caaaataagc taaatgaata taagcctaat aaaaatttat gtataggttt ataaaacttg
     3721 gctgttgtat aatgcctgta tttttgtgtc atccattcat cccatgtttt tttgggttcg
     3781 ctaagggtgt gtgccaaagg atctataaca atagcagtat tctttttatt ggtaacctta
     3841 ttaataaata aatcatcatc gccacttggt atcatgttga tggaagagaa ccctttattc
     3901 ctaaaaaaaa tatctttttt ataacttaaa tttctaccta cacccatgta tggtatgcca
     3961 gccaaagcat aagagaaata ttgtaatgcc gtatgaaaag tttcaaacct aatgagtttg
     4021 tttaaaatac cttttctttt tttaaaagca ccataaccta aaacaacttc aatatcatca
     4081 ttataagcat cttgcatata atacatccaa tcttcactgg ctggtaaaca atctgcatct
     4141 gttaataaaa cagtttcgta tttagcactt ttaattccaa tacttaatgg aaatttttta
     4201 ccctcaatca ttttagcttc ttgcgataac tcaatttggt taaggttttt aaaacttttc
     4261 ttaaactcat caagtaaata ttttgtttca tcaacagagt tatcgtttac aacaataacc
     4321 tcgtgtgtgg ttttatagtt ttgtactaaa atgcctggta aattattggc taagttatgg
     4381 gcttcatctc ttgcacaaat aataattgaa attggatgtt gaacagagtt tgctttattt
     4441 ttttttctat aaaatgcaag ccttgtaaag aaaaataaat aataaaataa ttggatagca
     4501 attacagaac aaaaaatata aaaaactatt gtaaatacaa taggagggat atcaattatc
     4561 ataacggcaa aaataattgt tgttccatct gaaagtggat aaaagtatga tttgtggata
     4621 agaaaaaaga ggagcaattt attataatgc cttaaaacca atttaaagca atattttctg
     4681 ctactttttc accatccata gcagcactaa caatgccacc agcatagcct gcaccctctg
     4741 aacaggggta taaatttttt aattgagggt gttgtaaagt tttactgtct cttggtattc
     4801 taacaggaga tgaagttctg ctttctgtag ctactattat tgcttcgttg gtataataac
     4861 ctttcatttt ctgtccaaaa atttgcaatg ctttttgtag ccttgtatat ataaatgcag
     4921 gtaaaactgc tgtaatatca ctactattta taccaggtaa ataggaacat ttgggtaaat
     4981 tagaagaaat tttattcgta caaaaatcta ctactctttg tgctggagca acaaaatttc
     5041 cacctccaaa agtataagcc ttttcttcca ctgctttttg aaaattcatt aatgcaagtg
     5101 gagaattatt ttgattccta cattctttaa tatccaaaat atctttcaat tcaacagaaa
     5161 caaccatgcc actgttagca aatgggttat ctcttttaga agggctccat ccatttacaa
     5221 ctaactcatt gttgttagta gctgctggag caatgatgcc acctggacac atgcaaaaac
     5281 taaatacacc tttgccattt acttgctcaa ccaaattata agttgctggt ggtaaaaaat
     5341 cacttctttt ttgtttttgg taattagtta aatgatattg tgctgtatca ataatatttt
     5401 gtgggtgttc aattcttact cctaaggcaa atggcttagc ctctataaat attttttttg
     5461 catataataa ttcataaata tctcttgcac tatgcccagt tgctaatatt acagcattag
     5521 caaatatttt ttcttctttg tttgaaacaa tgccttttac agtttcattt tctataatta
     5581 aatcagttaa ttttttttca aaatgcactt cgccaccgca atttataatt tgctctctca
     5641 ttgcagttat aatacttggt aatttgtttg tacctatgtg tgggtgggct tggtataaaa
     5701 ttttttcttc tgcaccaaac tgtacaaata aatttagtat tctgtctata cttcctcttt
     5761 tattgcttct tgtgtaaagt ttaccatcac tataggttcc agcacctcct tctccaaaac
     5821 aataattact ctcggtattt attttgcctt ctttatttaa tatggctaaa tctcttcttc
     5881 tacttcttac atctttacct ctttctacaa caattggttt tatacctaat tcaataagct
     5941 ttaatgctgc aaaaattcct gcaggtccag caccaactat aattactttt ttattactgt
     6001 tgctaacatc ttggaaatta attttttcaa tatttctttc aataaatttt tcgtttatga
     6061 atgcatttac tgtaatattt acccaaattt gttttctact tcttgcatct atactttgtt
     6121 ttaatttata aaaaccaaat acttcattgg gactcactcc aacagtttta gcaataattt
     6181 ctgtaatagt tgtattacta gcagcttccg atggtgtaat ttttaatgaa atttcttttt
     6241 gcatgaatta aaatataaat ggcgttacag agcctttagt aaaatttaaa tttcttaaaa
     6301 taaagttcac atttgtaaaa taaaatttat taccaaataa ttggttgaag tggcaaaaat
     6361 aatattatat ccatttgtat aattttgtat gataataaat ggcgaaattg ttaatactcc
     6421 ctccaaagca ataaattatt atgttataag gaactgttga taatttgata aataaatttt
     6481 ccagtagggc aaagagaaac tattactgtt gaagcattaa aaatacttta tgaaaaaaaa
     6541 taagcttctc caaaactctg cttagtaaaa gatatcttta catatattgt tatgactaaa
     6601 acagaattaa gaaaaattta taaggagaaa agaaaagcta tttcttcaaa agataaatta
     6661 aagtacgacg atttaatttt acttcaatta caaaaacttt actttgagaa tgtacaagtt
     6721 ttgcttacgt attggcctat ggagttagag ccaaatatgc aaatagtaac ctcgtacata
     6781 aagcacacaa attatgcctt aagagttgct tatcctgtta caaatttttc caacaatact
     6841 atgtgtgcaa ttggtgtaga ggatgataca agttttacta ccaatacttt tggtttaaca
     6901 gaaccgaaaa gtggcgagga aattaatgca aatgaaattg atattgtttt tgtgccacta
     6961 tttatttttg atttaagagg ttatagagtt ggctacggaa aaggctttta cgataaattc
     7021 ttagcatcgt gtaaaaatga tgttattaaa attgggtttt gttattttga acctatagaa
     7081 aaaattgatg ataccaataa ttttgatata cctttaaatt attgtattac accgcacaat
     7141 atttatgaat tttaatactt tatttttaaa atcatttagg gttttattat taccatttgc
     7201 cataatttat ggtatagtag ttagcataag aaattggtta tttaataaaa actattttaa
     7261 aagtgcaaaa tttaattttc cattaatttg tgttggaaac ttggcagttg gtggcacagg
     7321 aaaatcgcca atggttgagt atttagtaaa aatattacaa ccacatttta aaactgcaac
     7381 gcttagcaga ggttataaaa gaaaaacaaa aggctatgct ttggcaaata ctcaaactac
     7441 tgctttagaa attggtgatg aacctatgca atttcattta aaatttccag atgttgctgt
     7501 tgcagtggga gaagaaagat tggttgctat accgcagttg ttacatgata aacccgattt
     7561 agaagtaata gttttagatg atgcttttca gcatagatct gtaaatgcag ggctaaatat
     7621 tatacttact gaatatagca atttatatac acaagatttt tttcttccaa ctggagattt
     7681 gagagatgaa aaaagaagtg cagtaagagc agatatcatt gtggttacta aatgcccaac
     7741 atctattaca caagataaaa agtataaaat tttacgaagt attaaaccac ttaaacatca
     7801 aagagtattt tttacaacta ttgattatgg cactccatac catatttaca ataatcaaga
     7861 tgaatgggtt ttaacaccaa atgaagaggt gttattggtg tgcggaattg ctaacccaaa
     7921 accactaaaa gaatatttac atactgctgt acatacatac taccaaaaag attatagcga
     7981 ccaccatatt ttcaatattg atgatttgaa cgagataaaa caagtttttg ataaaataaa
     8041 agccaataga aaactaattt taactaccga aaaagatgct gtaagactaa tgaaattttc
     8101 ggaagtactt aaagatttgc cactttatgt attgccagtt aaacataaat ttttatttga
     8161 tgatacacag gtattccata acagaataat tgattttgta aataatttta gattagaatt
     8221 agatgaagaa aaataataaa cgccaacacc atactactaa ggaaacacat ttgcctacta
     8281 acactttaaa gggcatttta gaagtaacta atagtggtat tggttatgta attatgccag
     8341 aaggggtagg agatattttg gtaagacaag ataattttaa tactgcattt catggagata
     8401 ctgttagagt aaaagttatt aaagaaaatt tcaaatcagg taaaagagaa gggaaaataa
     8461 ctgaggttgt acaacgaaaa caaactgaat ttattggtaa actacagcta tctaatgcat
     8521 ttgctttttt tattccagat agcaataaac caatgccaga tttttttatt ccaaaaga
//
LOCUS       DAOQPK010000082         8439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_4607_length_8439_cov_4.773139, whole genome shotgun sequence.
ACCESSION   DAOQPK010000082 DAOQPK010000000
VERSION     DAOQPK010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8439
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4607_length_8439_cov_4.773139"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="PLU36_05375"
     CDS             <1..323
                     /locus_tag="PLU36_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMZ46214.1"
                     /translation="ASLKEVIIEITEKRLGATAVLDNNNHLKGIITDGDLRRMLEKND
                     QFLNLAAKDIVTTNPKTISPETMAVQALEVFKTFDITHLIVTENNQYLGILHLHDLLR
                     EGIV"
     gene            360..1442
                     /locus_tag="PLU36_05380"
     CDS             360..1442
                     /locus_tag="PLU36_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HMZ46215.1"
                     /translation="MNKHYYVAIMAGGIGSRFWPKSRTSMPKQFLDILNTGKSLIQWT
                     YERFAAFIPEENIFVVTSEDYEDIVAQQLPQLNKQNIVTEPSRKNTAPCIAYISYKLL
                     QKDPKAALIVAPSDHVILDVETFKNTCLKALNFVTQIKAFVTLGITPSYPNTGYGYIQ
                     HDTFSVAEGIYKVKTFTEKPNLELAKTFLASGDFLWNAGIFVWQVNNIIKAFEKFQPE
                     MFDLFENEKALFNSTKEKEAINRIYPLCTNISIDFAIMEKADNVYVMPSSFGWSDLGT
                     WNSAYENLEKDYLANAVVGNNVIIIDATKCMISATDNKLVLLQGLDDCIVVDTQDVLM
                     ICKKDKEQEIKEYVAEVKRIKGDIYL"
     gene            complement(1433..2002)
                     /gene="nadD"
                     /locus_tag="PLU36_05385"
     CDS             complement(1433..2002)
                     /gene="nadD"
                     /locus_tag="PLU36_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HMZ46216.1"
                     /translation="MNIGLYFGSFNPIHTAHLLIASYVADSALVDKVWFVVSPQNPLK
                     PSNSLLNEYDRLHLVNLAIENDNRFKAVDIEFRLPKPSYTIDTLTYLQEKYPTYTFYV
                     IIGADSYVNLPKWKNYEILKNNYPFIIYNRPGIELKQPLPKNFKILTAPLLEISSTEI
                     RKKIKAKKSILYMLPDAVRIEIERNGYYK"
     gene            complement(2018..2245)
                     /locus_tag="PLU36_05390"
     CDS             complement(2018..2245)
                     /locus_tag="PLU36_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HMZ46217.1"
                     /translation="MIKLSELAIGQKATIHSFEQNELYIKLMEMGCVPGELVKVEQIA
                     PLGDPISISIAGYSLSLRLSEAEEIFVEEIL"
     gene            2392..3957
                     /locus_tag="PLU36_05395"
     CDS             2392..3957
                     /locus_tag="PLU36_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase"
                     /protein_id="HMZ46218.1"
                     /translation="MQPKLFSLLFSTIFISTVIAQPPARTQPPATPASKEATTSSTAN
                     KSGNFPKNILDVDAKVEKNNEVTIKGKSVKYKVTAGTRPIWDKDGEVIAGIFYTFYER
                     TDVDKSTVRPIVFSFNGGPGSASVWMMLGYTGPKLLHIDEEGNPLQPYGLKDNPYSIL
                     DVADIVYVDPVNTGFSRPLGKSVSNETFFGVNADITYLAEWINSFVNKNKRWASPKYL
                     IGESYGTTRVSGLAMELQSRHWMYLNGVILVSPTDLGIKRDGPVADALTLPYYAATAW
                     YHKKLSADLQQKDLVNMLPEVEQFTINEYIPALTKGSQLTDVERNKIIEKVSKYSGLS
                     AKVVKENNLKISTGFFWKELLRDRGFTVGRLDSRYLGLDLKDAGNYPEYNSELVSWLH
                     SFTPAINLYLRDELGYETNLKYNMFGSVYPWNNERNTTGYNLASAMAQNPYLHVLVQS
                     GYYDGACDYFSAQYNMWQLDISNKLKNRISWEGYRSGHMMYLRKEDLAVANEHIRSFI
                     IKSTPAKTMPAKY"
     gene            4271..>8439
                     /locus_tag="PLU36_05400"
     CDS             4271..>8439
                     /locus_tag="PLU36_05400"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46219.1"
                     /translation="MRKIVLIFTIFQVLVVAVSAQSIFTRSTFTAAYSPITVIGGATE
                     GTLTGDDADQLSVPIGFTFSYNGIDRTTINLNTNGTAYFGSTAPTATIGRTNTNLYVT
                     ATNFNEVLAPWFDDLTEDATSSILYQTQGSPGSQTFTIQFTEYPHYYSGGTSQRLNHQ
                     IVLYEGTNIIEFRYGTKTGTDAISTSSSASIGLKWGAGDGFYLDAVTGSSNINNSMMT
                     PYKWPTYNYRFTPGAPSAPLSGTYNVGVGQTYNSLSDAVADLNHFGISAAVTFNLTDA
                     IYDSSSANGSNIFPILIGNVAGTSPTNTITISKTGSPATIMCRGTQSGNFGNKASATI
                     GSTSNEPMIGVFGSYITLNNLNFESHGTITPHAVDRAILVGNMSATIGAQNNTFTNIS
                     INLDRANSSGIGIQQNVLTIPTNATGANSNNIYRNLTIRDVYAGIYMLGNATYPDLST
                     EISTTACGTYNSIGDPLTPFDIGLSTGSSQNYGIRASNQKNFLIYNNEINNVGSTTTV
                     DGILVETFQGTSSVYNNKIANISNPSTSSTSSATGIRLTHPSSGTQDIRVYNNFINNI
                     TRGFTGTATVSRYIKGINISGTGGTATTSYYIDFNTVRLDGSSSPNCSSACFEIATTT
                     GPAFLVRNNIFANLTGAQGATAKHFGYFSTAVSTIGNTGSLLQYNDFYVPTDNGTSGH
                     VARGNTTNYNTVANMEAGFTTPASSTTNNLEVDPSFISPTDIHTSSPTLNNAADPAYA
                     TNTPWVTLDIDCATRNATTDIGADEFDVLALDMGVSAFVAPATAAQGCYTNAQTVTVT
                     IKNYAGVTIDFSVNPVTVTTNVTGAVITSLNAILNSGTLASGASMDVDMSTTLDMSTV
                     GTYTFNATTSVVGDGNLTNDDLPTAATRTKVALAAGTAKATPDSYCVTGGKPSLSLTG
                     HAGYSAIQWQESTTSGSGFTDILGGITSPYTVGTDITQTMYYQAILSCDASTVTSTED
                     TATLNNPLISTTTPGSRCGPGTVNLSATGTGSGINWYANPTGGVALGSGNSFTTPFIS
                     TTTTFYAAANNGYTTNSITGDGGWNHVTATGSYQTSLITTAYMILTVSKDLTLSSLDI
                     YPSATVGTSFGLEARTVSQSGPTFASYSGTTTIQNGGTPTVAQTVPVNWVLPIGTYYI
                     GFTGTNPNTWRSGSFAHGLPWTEPGLASMNFSLSPSYQYYFYNLQLTTGCESSPRTAV
                     TATINTPPAIDVTTSGPANICVSGSSTLNVTSSNAGYTYTWMPGSLPGTGVVVNPAST
                     TKYYANAVDNSGGPNDGCSAIDSFTVTVQNPQINEVTPAYAYSCSGSGAKLDVLVSKT
                     LGTQSNASAPTGTEPNVFQYYYEGNKQQSIVLASELNALGISGITTINKIGFQMTAAN
                     FLSFSNVIIKMG"
BASE COUNT         2872 a         1428 c         1423 g         2716 t
ORIGIN      
        1 atgcctcttt aaaagaggtg attatagaaa taaccgaaaa aagattaggt gccactgccg
       61 tgttagataa taataaccat ttaaaaggca ttattacaga tggagatttg agaagaatgt
      121 tagaaaagaa tgaccaattc ttaaatttag ctgcaaaaga tattgtaact acaaacccaa
      181 aaacaatatc tcccgaaaca atggctgtgc aagctttaga ggtatttaaa acttttgata
      241 ttacccattt aattgttaca gaaaataacc aatatttagg tattttacat ttacatgatt
      301 tattaagaga agggattgtt taaaatatac gtttatttgc acttcttatt ttttgctata
      361 tgaataaaca ttattacgtt gcaataatgg ctggcggaat tggtagtagg ttttggccta
      421 aaagcagaac ctctatgcct aaacaattct tagatatttt aaatacaggc aaaagcttaa
      481 ttcaatggac ctatgaaagg tttgcagcat ttattcccga agaaaatatt tttgttgtta
      541 cttccgaaga ttatgaagac attgttgctc aacaattgcc acaattaaat aagcaaaata
      601 ttgttacaga accaagcaga aaaaatactg ccccttgtat tgcttatata tcttataaac
      661 ttttacaaaa agaccccaaa gctgcattaa ttgtagcacc cagcgatcat gtaattttag
      721 atgtagaaac ctttaaaaat acttgtttaa aagcattaaa ttttgttaca caaattaaag
      781 cttttgttac tttaggcata actccatctt acccaaatac aggctatggt tatatacaac
      841 acgatacttt tagtgttgcc gaaggtattt ataaagtaaa aacttttacc gaaaagccaa
      901 atttagaatt agcaaaaacc tttttagcaa gtggagattt tttatggaat gcaggcatat
      961 ttgtttggca ggtaaacaat attattaagg cattcgaaaa atttcaacca gaaatgtttg
     1021 acctttttga aaatgaaaaa gcacttttca attctacaaa agaaaaagaa gcaattaatc
     1081 gtatctatcc tttatgtacc aatatttcta tagattttgc cattatggaa aaagcagata
     1141 acgtttatgt tatgccttcc tcatttggct ggagtgattt aggaacctgg aacagtgctt
     1201 atgaaaattt agaaaaagat tatttagcca atgcagtagt aggcaataat gtaattatta
     1261 ttgatgctac caaatgtatg attagtgcta cggataataa attagtgcta ttgcaaggtt
     1321 tagatgattg tattgtagta gatactcaag atgtattaat gatttgcaaa aaagataaag
     1381 aacaagaaat taaagaatat gttgcagagg taaaaagaat aaaaggagat atttatttat
     1441 agtaaccatt cctttctatt tctattctta cagcatcggg caacatatat aaaatacttt
     1501 ttttagcttt tatttttttt cttatttcgg ttgaggaaat ctctaacagt ggagcagtaa
     1561 gtattttaaa attttttggt aatggttgtt ttaactcaat gccaggtcta ttataaataa
     1621 taaaaggata gttatttttt agtatttcgt aattcttcca tttgggcaag tttacataac
     1681 tatctgcacc aataattaca taaaaagtat aagtaggata tttctcttgt aagtaagtta
     1741 aagtatcaat tgtataagat ggtttgggta atctaaattc aatatcaaca gctttaaacc
     1801 tgttatcatt ttctatagct aaattcacta aatgtaacct gtcatattca tttaataaac
     1861 tattagaagg ttttaatggg ttttgcggag aaacaacaaa ccaaacctta tccactaaag
     1921 cactatctgc cacataactt gcaattaata aatgggcagt atgaatgggg ttaaaagagc
     1981 caaaatacaa tccaatattc attgtagata aattcagtta tagtatttct tccacaaaaa
     2041 tttcttctgc ttcactaagc cttaaactta aagagtagcc agcaatactg atagaaatag
     2101 gatcgccaag tggtgcaatt tgctctactt taactaactc tccaggtaca caacccattt
     2161 ccattaattt aatatacaac tcattttgtt caaaagaatg aatggttgct ttttggccta
     2221 ttgctaattc gctcaattta atcattccac aatttttatt gctgcaaatg tagttatata
     2281 ccagtagtga tgaagattta agattaatct tttttctacc actcatacaa tcattttcat
     2341 ctttcttaac taaaaattat acctttcatt ttaagaaaaa atataaccaa tatgcaacca
     2401 aaattattta gtttattgtt cagtacaata ttcataagta ctgtaattgc tcaaccacca
     2461 gcaagaacac agccacctgc aacacctgca tctaaagagg ctacaacatc ttcaactgca
     2521 aacaagtcgg gtaattttcc taaaaatatt ttagatgtag atgcaaaagt tgaaaaaaat
     2581 aatgaggtaa ctattaaagg caaatcagta aaatataagg taactgctgg tacaagacct
     2641 atttgggata aagatggaga agtaattgcc ggtatttttt atacttttta tgaaagaacc
     2701 gatgtggaca aaagtactgt acgtcccata gttttttcat ttaatggtgg cccaggaagt
     2761 gcaagtgtat ggatgatgct tggctataca gggccaaagc ttttacatat tgatgaggaa
     2821 ggaaatcctt tacaacctta tggcttaaaa gataacccgt attctatttt agatgttgcc
     2881 gatattgttt atgtagatcc tgtaaataca ggcttttcaa ggccattagg aaaatcagtt
     2941 agtaacgaaa ctttctttgg tgtaaatgca gatattacct acctggcaga atggataaat
     3001 agctttgtaa ataaaaataa aagatgggct tcgccaaaat atttaattgg cgaaagctat
     3061 ggcacaacaa gagtttctgg gcttgctatg gaattacaaa gcagacattg gatgtattta
     3121 aacggagtaa ttttagtttc tccaaccgat ttaggtatta aaagagatgg accagttgca
     3181 gatgctttaa cccttcctta ttatgctgct actgcatggt accataaaaa actatcggca
     3241 gatttacaac aaaaagattt agtaaatatg ttgccagaag tagaacaatt tactattaac
     3301 gaatatatac ctgcattaac caaaggcagc caacttacag atgtagaaag aaacaaaata
     3361 atagaaaaag tgagtaaata ctcaggctta tctgccaaag tggtaaaaga aaataattta
     3421 aaaatttcta caggcttttt ttggaaagaa ttattaagag atagaggttt tactgtagga
     3481 aggttagact ctagatactt aggattagat ttaaaagatg caggaaatta ccctgaatac
     3541 aactccgaat tagtttcttg gttacatagt tttacacctg ccattaacct atatttaaga
     3601 gatgaattag gctatgaaac taatttaaaa tacaatatgt ttggctcggt atatccttgg
     3661 aataatgaga gaaatactac aggttataac cttgcaagtg ctatggccca aaacccatat
     3721 ttacatgttt tagtgcaaag tggttattac gatggggctt gcgattattt tagtgcccaa
     3781 tataatatgt ggcaattaga tatttctaat aaacttaaaa atagaatttc ttgggagggg
     3841 tatagaagtg gccacatgat gtatttaaga aaagaagatt tggcagttgc aaacgaacat
     3901 ataaggtcat ttattattaa atctactcca gctaaaacta tgcctgcaaa gtattaataa
     3961 atttattttt acaatcagtt tttttatagc caatgacatt atttgcattg gcttttttat
     4021 taaacaaatt atctagttca aaaaaataaa aaaaatttat actctaatgc agcatttaat
     4081 taaatattaa aatctcctta tctgaattgg atattgaaat atattctaac aaaaaaaaat
     4141 tttttgttaa agaaaatata atataaattg caattcacaa atccatcata ctactgataa
     4201 acttggtcta aagtttaaaa cccgctattc ttcatttaaa ccctatttaa tttacctaaa
     4261 tctatcaatt atgagaaaga ttgtactcat ttttacaatt ttccaagtat tggttgttgc
     4321 tgtatctgca caatcaatat ttacaagaag cacatttact gcagcttact ctccaataac
     4381 tgttattggt ggtgctacag aaggtacatt aactggcgat gatgcagacc agctttctgt
     4441 acccattggt tttacattca gctataatgg tatagatcgt actaccatta atttaaacac
     4501 taacggaaca gcatattttg gcagcacagc accaactgca accatcggta gaacaaatac
     4561 taatttgtat gtaactgcca caaattttaa tgaagttttg gctccttggt ttgatgattt
     4621 gacagaagat gctacctcat ccattttata tcaaacacaa ggttcaccag gtagtcaaac
     4681 ctttactatt cagtttactg agtacccaca ttattactca ggaggtacat cacaaagatt
     4741 aaatcatcaa attgtgttgt acgagggtac taatattatt gagtttagat atggcactaa
     4801 aactggtaca gacgctattt caacaagttc ttctgcatct atagggttaa aatggggtgc
     4861 tggcgatggc ttttatttag atgctgttac tggtagttcc aatattaata atagtatgat
     4921 gactccttat aaatggccaa catataatta taggtttact cctggtgcac cttctgctcc
     4981 tttaagtggt acctataatg taggcgttgg gcaaacttac aatagcctat ctgatgccgt
     5041 ggcagacctt aaccattttg gtatttctgc tgctgtaact tttaatctaa ccgatgctat
     5101 ctacgactct tccagtgcaa atggttctaa tatttttcct attttaattg gtaatgtagc
     5161 tggcacaagt cctaccaata caattacaat atctaaaaca ggttctcctg caactattat
     5221 gtgccgtggt acacaaagcg gtaattttgg aaataaagca agtgcaacta ttggaagtac
     5281 aagcaatgag cctatgattg gtgtttttgg gtcttatata actttaaaca atttaaactt
     5341 tgaaagccat ggtactatta caccacatgc agtagatagg gcaattttgg taggtaatat
     5401 gtctgccact attggtgctc aaaacaatac atttaccaat atttctatta acctagatag
     5461 agctaatagt agtggtattg gcattcaaca aaacgtactc actataccta ccaatgcaac
     5521 aggtgcaaac tccaataata tttatcgtaa tttaactata agagatgttt atgcaggtat
     5581 atatatgtta ggaaatgcaa catatccaga tttaagcact gaaataagta ctactgcttg
     5641 tggtacttat aatagtatag gagatccatt aactcccttc gatattggat tatcaactgg
     5701 gtcaagtcaa aattatggta taagagccag taatcaaaaa aacttcctta tatataataa
     5761 tgaaattaac aatgtaggct ctacaaccac tgtagatggt attttggtag aaacatttca
     5821 aggcacaagc tctgtttata ataacaaaat tgccaatata agtaacccct ctacttctag
     5881 tacaagttct gcaactggta ttaggctaac acacccttca tctggtacgc aagatatcag
     5941 ggtttacaat aattttatca ataatataac ccgtgggttt acaggtacag caactgttag
     6001 caggtatatt aaaggtatta atatttctgg tactggtggt actgccacta ccagttatta
     6061 tatagatttt aatacagttc gtttagatgg ctctagttct ccaaactgct ctagtgcatg
     6121 ctttgaaatt gcaactacta ctggtcctgc ttttttagtt agaaataata tttttgcaaa
     6181 tttaactggt gcacagggtg caactgccaa acactttggg tattttagta cagcagtaag
     6241 tacaataggt aataccggtt cattattaca atacaacgat ttttatgtac ctactgataa
     6301 cggtactagt gggcatgttg caagaggaaa tacaacaaat tataatacag tggctaatat
     6361 ggaggctggg tttacaacac ctgcttcttc tactactaat aatcttgaag ttgacccatc
     6421 ctttatatcc ccaacagata ttcatacttc atctccaaca ttaaataatg ctgccgatcc
     6481 tgcttatgca accaatacac cttgggttac cttagatatt gattgtgcca caagaaatgc
     6541 aacaactgat attggagccg atgagtttga tgtacttgcc ttagatatgg gtgtgtctgc
     6601 atttgtagct cctgctactg cagcacaagg ttgttatacc aatgcacaaa cagtaactgt
     6661 tactattaaa aactatgctg gcgtaacaat agacttttct gttaatcctg ttacagttac
     6721 aacaaatgtt actggtgcag ttataacctc tttaaatgca attcttaata gtggcacttt
     6781 ggcatctggg gcttctatgg atgtagatat gtccaccaca ttagatatga gcacagttgg
     6841 cacttatacc tttaatgcaa caacaagtgt tgtaggagat ggtaacctta ccaacgatga
     6901 tttacctaca gctgcaacca gaacaaaagt agcacttgca gcaggcactg caaaagcaac
     6961 acccgatagc tattgtgtaa caggtggtaa gccaagttta tctttaacag ggcatgctgg
     7021 ttatagtgcc atacaatggc aagaaagtac tacttctggc tctggattta cagatatttt
     7081 aggtggtatt acaagtccat atactgtggg tacagatatt actcaaacta tgtattatca
     7141 agccatactt tcttgtgatg catcaacagt aacttctact gaagatactg ccactctaaa
     7201 caacccacta atcagtacta caacaccagg cagtcgttgc ggaccaggta ctgtaaactt
     7261 aagtgctaca ggaaccgggt ctggaataaa ttggtatgct aatccaacag gtggtgtagc
     7321 attaggctct ggaaattcat ttacaacacc ttttataagc accacaacaa ctttctatgc
     7381 tgctgctaat aatggttata caacaaatag cattactgga gatgggggct ggaaccatgt
     7441 tacagcaact ggtagctacc aaacatctct tataactact gcctatatga tactaacagt
     7501 atcaaaagat cttactttat cttcattgga tatttatcct tctgcaactg taggtacctc
     7561 atttggatta gaagcaagaa cagttagtca aagcggacca acctttgcaa gttactctgg
     7621 aactacaaca atacaaaacg gcggtacccc aacagtagca caaacagtgc ctgtaaattg
     7681 ggtgttacct ataggtacct attatattgg tttcacaggt actaatccaa atacctggag
     7741 gtcaggatct tttgcccatg gtttaccatg gacagagcct ggtttggctt ctatgaattt
     7801 ctctctatct cctagctacc agtattattt ttataattta caacttacaa caggttgcga
     7861 aagtagccca cgtactgcag ttactgcaac aataaatact ccacctgcca ttgatgtaac
     7921 aacttctggt cctgccaata tttgtgtaag cggtagcagt accttaaatg ttacaagtag
     7981 taatgcaggt tatacttata cttggatgcc aggcagttta ccaggtacag gagttgtagt
     8041 taatccagct tctactacca aatattatgc aaatgcagta gataacagtg gcggaccaaa
     8101 tgatggttgc tctgctattg attcatttac tgtaaccgta caaaacccac aaattaatga
     8161 ggtaacacct gcctatgcct atagttgcag cggtagtggt gcaaaacttg atgtacttgt
     8221 tagcaaaaca ttaggtactc aatctaatgc ttcagcacca actggtacag agcctaatgt
     8281 atttcaatat tattacgaag gaaataagca acaaagtatt gttttagcat ccgagttaaa
     8341 tgctttagga atttcaggta tcactactat taataaaatt ggtttccaaa tgacggctgc
     8401 taattttctt agtttctcaa atgttattat aaaaatggg
//
LOCUS       DAOQPK010000083         8205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_4827_length_8205_cov_4.002454, whole genome shotgun sequence.
ACCESSION   DAOQPK010000083 DAOQPK010000000
VERSION     DAOQPK010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8205
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4827_length_8205_cov_4.002454"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="PLU36_05405"
     CDS             complement(<1..548)
                     /locus_tag="PLU36_05405"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HMZ46220.1"
                     /translation="MVQKLVAFYGDTNTYTHVNKNNFFIEASNGHYVCWVRTEKDNSI
                     AAVEVFTVEDEANFDWNDIFYEIGKQSSLHDKSYNLVTIFYHNNENVLVPAHKFNASN
                     ADAYLDLMFGAKENTVVKYDSVDFGGELMYNVYRVNALLNTMVKTNFLTVTEFHHYSG
                     LLKHLTIEDKKLQGNNINVYFY"
     gene            complement(870..2030)
                     /gene="dnaJ"
                     /locus_tag="PLU36_05410"
     CDS             complement(870..2030)
                     /gene="dnaJ"
                     /locus_tag="PLU36_05410"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HMZ46221.1"
                     /translation="MSKRDYYEILGVSKSSSPDEIKKAYRKVAMQYHPDRNPGDKAAE
                     EKFKEAAEAYEVLSDTDKKAKYDRYGHQAFGPGTGGGGFHNTNMEDIFSHFGDIFGDG
                     MFGDFFGGGRRSSGAGARTRGQRGSNLRIKLKLTFAEIANGVTKQVKVKKYIICTTCG
                     GNGAKDKNSLQTCTTCGGNGQVRKVQNTFLGQMQTVTTCSTCNGEGSTVTSKCTACKG
                     EGRVYGEETISIDIPAGVQAGMQLSMSGKGNAGERGGSYGDLIIQIEEEQHTDLQRDG
                     LNVAYDLHISFPEATFGTNIEVPTIDGRAKIKIPPGTQSGKIFRLKGKGFPEVQGYGR
                     GDQLVNVNVWTPQNLTSEEKAILEKLNNSPNFKPQPSKNDKSFFEKIREAFN"
     gene            complement(2069..2659)
                     /locus_tag="PLU36_05415"
     CDS             complement(2069..2659)
                     /locus_tag="PLU36_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HMZ46222.1"
                     /translation="MYNTPKPNKMDMEEKDIKANEATESTMNINADENAAGTTHLNDN
                     VEESAVDKLKVELNEQKDKYLRLAADFDNYRKRTAKEKYELIDSASKDVILSLLDVLD
                     DIDRAEIQLENTTDFEQNKEGVKLVFNKLKSALQSKGLKSFESIHTDFDVEKHEAISE
                     IDAPSNNLQGKVLDEIQKGYYIKDKIIRFAKVVVGK"
     gene            complement(2751..3845)
                     /gene="carA"
                     /locus_tag="PLU36_05420"
     CDS             complement(2751..3845)
                     /gene="carA"
                     /locus_tag="PLU36_05420"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HMZ46223.1"
                     /translation="MSLTNNTAILLLNDGTIFYGKAFGKIGTTTGELCFNTGMTGYQE
                     VFTDPSYTGQILIMNNVHVGNYGIKENEVESDSIKINGLIGRNLEEQFSRIQANQSMQ
                     EYLAQNNLVAIEGVDTRALVAHVRTKGAMNCIISSDEQDITILINKLANCPNMDGLEL
                     ASTISTNQPYELGNKEAEIKIAVLDFGIKKNILNCLVERGVSVKVFPATTSISELKAY
                     NPNGYFLSNGPGDPAAMQYAINTVKEILEENKPVFGICLGHQLLALANGIATFKMHHG
                     HRGLNHPVKNLITGKCEITTQNHGFGVQPNSVKENELVEITHINLNDESIEGIRLKNK
                     PAFSVQYHPEATPGPHDSRYLFDDFIAMMQ"
     gene            complement(3944..4456)
                     /gene="rplQ"
                     /locus_tag="PLU36_05425"
     CDS             complement(3944..4456)
                     /gene="rplQ"
                     /locus_tag="PLU36_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HMZ46224.1"
                     /translation="MRHGDKINNLGRKKAHREALLSNLASQLLVHKRIVTTLAKAKAL
                     RTYIEPLITKTKKGDDKNALSHQHRIVFSYLQDKAATKELFTVIAPKVVSRPGGYTRI
                     IKLGIRPGDNAEKAMIELVDFNEIYTKGAVKAAEPAKKTRRSAGAKKKATETTSASET
                     TTETENKTEE"
     gene            complement(4539..5540)
                     /locus_tag="PLU36_05430"
     CDS             complement(4539..5540)
                     /locus_tag="PLU36_05430"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HMZ46225.1"
                     /translation="MAILNFQKPDKIVLQKATDFEGQFEFRPLEPGYGLTVGNALRRV
                     LLSSLEGYAITGIKIDGVDHEFATIKGITEDVTEIILNLKQVRFKKKVEHDVTNEKLT
                     LSIKGNTEFNAGNIGDASLNFEVMNPQLVICTMDNTAKLDIELTINKGRGYIPAEDNK
                     VKDTPTGYIAIDSIHTPIKNVKYSIENYRVEQRTDFEKLILEVATDGTIHPEEAVKQA
                     SRILIQHLMIITDENITFDNKEDKKEDVVDEQVLQLRKVLKTPLEDLDLSVRAFNCLK
                     AAKINSLSELVQYEQEDLMKFRNFGQKSLSEIEQVLTERGLNFGMDLVKLGLDNLDN"
     gene            complement(5671..6276)
                     /gene="rpsD"
                     /locus_tag="PLU36_05435"
     CDS             complement(5671..6276)
                     /gene="rpsD"
                     /locus_tag="PLU36_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HMZ46226.1"
                     /translation="MARYTGPKTKISRIFGEPILGNGKWLTKNSNPPGQHGAAKKRKT
                     LGEYALQLREKQKAKYTYGVLERQFRKTFEEASRMKGVKGENLIKLLEARLDNTIYRM
                     GLAASRPEARQLVAHKHITVNGEVMNIPSYSCKAGDVITYRAKSEHNSSITSKSRGKN
                     PKFSWLDWNETEKKGTFITYPERESVPENIKEQLIVELYSK"
     gene            complement(6399..6809)
                     /gene="rpsK"
                     /locus_tag="PLU36_05440"
     CDS             complement(6399..6809)
                     /gene="rpsK"
                     /locus_tag="PLU36_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HMZ46227.1"
                     /translation="MAKPAKAQNSAKAAAKKRVVKVDAAGEVRINATFNNIIISFTNK
                     SGQVISWSSAGKMGFRGSKKNTPYAAQMAAADAAKVALDAGLKKVDVFVKGPGSGREG
                     AIRSIAQNGIDVTSIKDVTPIPHNGCRPPKKRRV"
     gene            complement(6942..7322)
                     /gene="rpsM"
                     /locus_tag="PLU36_05445"
     CDS             complement(6942..7322)
                     /gene="rpsM"
                     /locus_tag="PLU36_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HMZ46228.1"
                     /translation="MARIAGIDLPKNKRGEIGLTYVYGIGRSTAQYILTKAGIDFDKK
                     VNQWNDDEQAAIRNIINEEFKVEGALRSETSMNIKRLLDIACYRGLRHRKGLPVRGQR
                     TKTNSRTRKGKRKTVAGKKKAPKK"
     gene            complement(7360..7476)
                     /gene="ykgO"
                     /locus_tag="PLU36_05450"
     CDS             complement(7360..7476)
                     /gene="ykgO"
                     /locus_tag="PLU36_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HMZ46229.1"
                     /translation="MKVRASIKKRSADCKIVKRGGKLYVINKKNPRFKQRQG"
     gene            complement(7525..7743)
                     /gene="infA"
                     /locus_tag="PLU36_05455"
     CDS             complement(7525..7743)
                     /gene="infA"
                     /locus_tag="PLU36_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HMZ46230.1"
                     /translation="MSKQQLIKQDGIILEALSNAMFRVKLENGHEILATISGKMRMHY
                     IRILPGDKVGVEMSPYDLSRGRINFRYK"
     regulatory      8063..>8205
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT         2429 a         1458 c         1117 g         3201 t
ORIGIN      
        1 tggtagaaat aaacatttat attattacct tgtaattttt tatcttctat agttaagtgt
       61 ttaagtaacc cagaatagtg gtgaaattcg gtaacagtta aaaaatttgt tttaaccatt
      121 gtatttaata atgcatttac tctgtacaca ttgtacataa gttcgccacc aaaatcaaca
      181 ctatcatatt ttactacagt attttcttta gctccaaaca ttaaatctaa gtaagcatcg
      241 gcgttagatg cattaaattt atgtgcaggc actaaaacgt tttcgttatt atggtaaaaa
      301 attgttacaa ggttatagct tttatcatgt aacgatgact gtttgcctat ttcataaaaa
      361 atatcattcc agtcaaaatt cgcttcatct tcaacagtaa atacttctac tgcagcaata
      421 ctattatcct tttctgttct tacccaacat acgtaatgcc cattacttgc ctctataaaa
      481 aagttatttt tatttacatg agtgtaggta tttgtatcac cataaaaggc tactaatttt
      541 tgtaccataa tatgctattg ctatgcaatt atataaaata aatactgtat tagaaaaggc
      601 taaagtacaa ataatataat aggaagagtt tgaaaataag tcaatatact ttgatttttt
      661 gattttttga agaatagtat ttaaagttta ggggcaggtt tatactattg atatttaaaa
      721 attaaaacag ttaaatagta ttatttcctt tttataaatt gcttcatggg ctttacaact
      781 aattagttta aagtaaagcc ataattcata tctatattaa aatccagagg aggaaacatt
      841 aaaaaagcct tttacaaggc ttaaaaatgt taattaaatg cttcccgaat tttttcaaaa
      901 aaacttttat cgtttttaga aggctgaggt ttaaaattag ggctattgtt taatttttct
      961 aatatcgctt tctcttcaga tgttaagttt tgaggagtcc aaacatttac atttactaat
     1021 tggtcgcctc taccataacc ttgcacttca ggaaatcctt tccctttaag cctgaaaatt
     1081 ttgccacttt gtgtacctgg cggaattttt atttttgctc ttccgtcaat agttggaact
     1141 tctatattgg ttccaaaagt tgcttcagga aatgagatat gtaaatcgta tgcaacgttt
     1201 aatccatctc tttgtaaatc ggtatgttgt tcttcttcta tttgaataat taaatcgccg
     1261 taagaccctc ctctttctcc agcattgcct ttaccactca tactaagttg catacctgct
     1321 tgtacgcctg ctggtatatc aatactaatt gtttcttctc catatactct cccttctcct
     1381 ttacatgctg tacattttga agtaactgta gagccttctc cattacatgt agagcaagtg
     1441 gttacggttt gcatttgacc taagaaagta ttttgcactt tacgaacttg tccattacca
     1501 ccacaagttg tacatgtttg taaactattt ttatcttttg ctccattgcc accacaagtt
     1561 gtacaaataa tatatttttt taccttcact tgtttggtta caccgtttgc aatttctgca
     1621 aaagtgagct ttaattttat tcttaaattg ctacctcttt ggcctcttgt tctagcacca
     1681 gccccactac ttcttcttcc tccaccaaaa aaatctccaa acattccatc tccaaatata
     1741 tctccaaaat ggctgaaaat atcctccata ttagtgttat gaaagcctcc accacctgtt
     1801 cctgggccaa aagcctgatg gccatatcta tcgtattttg cttttttatc tgtatcgctt
     1861 agtacttcat acgcctcggc tgcttcttta aatttttctt cggctgcttt atctccaggg
     1921 tttctatctg ggtgatattg catagcaact tttctatatg cttttttaat ttcatcgggc
     1981 gaggatgatt ttgacacacc taatatttcg taataatctc tttttgacat ggcttgttgg
     2041 tttgtgtttc aaacactaaa gttttattct attttcctac tacaactttt gcaaaacgaa
     2101 taattttatc ttttatataa tacccttttt gaatttcgtc taaaactttt ccttgtaagt
     2161 tgttgcttgg tgcatcaatt tctgaaatag cttcatgttt ttctacatca aaatctgtat
     2221 gtatgctttc aaaagatttt aatcctttgc tttgtaaagc tgactttaac ttattaaata
     2281 ctagtttaac gccttcttta ttttgttcaa aatctgttgt attttctaat tgtatttcag
     2341 cacggtcaat atcatctaaa acatctaata aagaaagaat aacatcttta ctggcagagt
     2401 ctattaattc atatttttct tttgcagtcc tttttctgta gttatcaaaa tcggcggcta
     2461 atcttaagta tttatctttt tgttcattta attctacttt tagtttatct acagcacttt
     2521 cttccacatt gtcattcaaa tgtgtagtac ctgctgcatt ttcgtcagca ttaatattca
     2581 tggtgctttc tgtggcttca tttgccttta tatctttttc ttccatatcc attttattgg
     2641 gtttaggagt attgtacaaa tagtttgccg ttaaaaggaa taggaaaaaa tgacataact
     2701 acaatctata aactacaaat aatgtattac tacacgatga taaaatatgc ttattgcatc
     2761 attgcaataa aatcatcaaa taagtatcta ctatcatgag ggccaggtgt agcttctgga
     2821 tggtattgaa cagaaaatgc cggcttgttt ttaagcctta ttccttcaat actttcatca
     2881 ttcaaattta tatgagttat ttcaaccaat tcattttctt ttactgaatt tggttgtaca
     2941 ccaaaaccat gattttgtgt tgttatttca catttacctg taattaagtt ttttacagga
     3001 tggtttagcc ctctatgtcc atgatgcatt ttaaatgttg caattccgtt tgctaatgcc
     3061 aataattgat gccctaaaca aataccaaat acaggtttgt tttcttctaa aatttctttt
     3121 actgtattaa tggcatactg cattgcagca gggtctcctg ggccattaga taggaaatat
     3181 ccattaggat tatatgcttt taattcgcta atagaagttg tagctggaaa aactttaacg
     3241 cttactcccc tttccactaa acagtttaaa atatttttct taatgccaaa atctaaaact
     3301 gcaattttta tttctgcttc tttattaccc aactcataag gttgattagt acttatggtg
     3361 cttgctaatt ctaaaccatc catattgggg caattagcta atttatttat taaaattgta
     3421 atatcttgtt cgtctgagga tataatacaa ttcatagcac ctttggttct tacgtgtgcc
     3481 accaatgctc ttgtatctac cccttcaata gcaaccaaat tattttgtgc taaatattct
     3541 tgcatagatt ggttggcttg tattctgcta aattgttctt ctaaatttct tcctattaat
     3601 ccgtttattt taatactatc gctttctact tcattctctt taattccata attaccaaca
     3661 tgaacattat tcattattaa gatttgaccg gtataactag ggtctgtaaa tacttcttga
     3721 taacctgtca tacctgtatt aaaacaaagc tcaccagttg ttgtaccaat ttttccaaat
     3781 gctttaccat aaaaaatggt accatcattt aaaagtaaaa ttgctgtatt attagttaag
     3841 gacatgatgt tttaaatggt tttgaatatt gcaaaataaa aaaggcaaga agtaatttcc
     3901 ttgccttttc ttgaaaactt ttgaacattt aattaaatta aaattattct tcggttttat
     3961 tttcagtttc tgtagttgtt tctgaagcac tagtagtttc agttgctttc tttttagctc
     4021 ctgcacttct acgagttttc ttagcaggtt ctgctgcttt aactgctcct ttagtgtaaa
     4081 tttcattgaa atctactaat tcaatcattg ctttttctgc attatcacca ggcctaatgc
     4141 ctaatttaat aattcttgta taaccacctg ggcggctaac aactttaggt gcaattacag
     4201 taaacaattc ttttgtagca gctttatctt gtaaataact aaatacaata cgatgttgat
     4261 ggcttaaagc atttttatca tcgccttttt tagtttttgt aattaaaggt tcaatataag
     4321 tacgtaatgc tttagcttta gctaatgtag ttacaatacg tttatgaact aataattgag
     4381 aagctaaatt gcttaaaagg gcttctctgt gtgccttttt acggcctaaa ttgtttattt
     4441 tatctccgtg acgcatgact tttattttgt agcttatacc gtgtcaggaa ttataagctt
     4501 atgattaaat aatttaagca aatgcttaaa ctgttttatt aattatctaa attatctaaa
     4561 ccaagtttta ctaaatccat accaaaattt aaacctcttt cggttaaaac ttgttctatt
     4621 tctgataatg atttttgacc aaagtttcta aacttcatca agtcttcttg ttcgtattgt
     4681 actaactcgc taagagagtt aatttttgct gctttcaaac aattaaaggc tcttactgaa
     4741 aggtctaaat cttctaaagg tgtttttaat actttacgca attgtaacac ttgttcatct
     4801 actacatctt cttttttatc ttctttgtta tcaaaagtga tgttttcatc agttataatc
     4861 atcaagtgtt gaattaaaat acgagatgct tgtttaactg cctcttcagg atgaatagta
     4921 ccatcagtag caacttctaa aattaatttt tcaaagtcag ttctttgttc tacacgataa
     4981 ttttctatgg aatattttac atttttaata ggtgtatgta tggagtcaat agcaatatat
     5041 ccggtaggtg tatcttttac tttattatct tctgcaggta tataaccacg gcctttatta
     5101 atagttaatt caatatctag ctttgcagta ttatccatag tacaaattac taattgcgga
     5161 ttcattactt caaagtttaa agaagcatct cctatatttc ctgcattaaa ctcggtattt
     5221 ccttttatag ataaagtgag tttttcgttg gtaacatcat gctccacttt tttcttaaat
     5281 ctaacttgtt taagatttaa aataatttca gtaacgtctt cagtaatacc tttaatagta
     5341 gcaaactcat ggtcaacacc atcaatttta atacctgtaa tagcgtaccc ttcaaggctg
     5401 cttaaaagta ctctacgtaa agcattacca acggttaaac cataccctgg ctctaacggg
     5461 cgaaattcaa attgtccttc aaaatcagta gctttttgta aaacaatttt atccggcttt
     5521 tggaagttta aaatggccat tttatattcc tgtttttatt tgatttaaaa aaaatttgtt
     5581 tccatcttag gtgcaatatt caactttagt tgcgtcgcac acttgtacag ttgtaagtgt
     5641 attcaattat agcagtaata ctgttataaa ttatttagaa tacaactcca ctatcaattg
     5701 ttctttaata ttttctggaa ctgattctct ttcaggataa gtaataaatg ttcctttctt
     5761 ctcagtttca ttccaatcta accagctaaa cttagggttt ttaccacggc ttttgctagt
     5821 tatagaacta ttgtgttcgc tttttgcacg atatgtaata acatctcctg ctttacaaga
     5881 gtaagatggt atgttcatta cctctccatt aacagttatg tgcttatgag caactaattg
     5941 tcttgcttct ggacggcttg cagctaaacc catacgatag atagtgttgt ctaaacgagc
     6001 ttctaataat ttaattaaat tttcgccttt aacacctttc atacgagaag cctcttcgaa
     6061 agtttttcta aattgacgtt ctaaaacacc ataagtatat tttgcttttt gcttttctct
     6121 aagctgcaaa gcatattcac ctaatgtttt acgttttttt gcagcaccat gctgaccggg
     6181 agggttacta tttttagtta accatttgcc attgcctaaa attggctctc caaaaattct
     6241 agaaattttg gtcttaggac cagtgtaacg tgccataatt tttatttcat ttcgattttt
     6301 tttgaatcgg taaaactatc ggttaaaaat cgtaaaaaat aaatagttta ccctttgata
     6361 taaatgaaac ctgaacggct caatataaaa tttataaatt aaactcttct ctttttaggt
     6421 ggtctacaac cattgtgagg tataggtgtt acgtctttaa tagatgttac atcaatacca
     6481 ttttgtgcaa tacttctaat tgcaccttca cgtccagaac ctggaccttt tacaaataca
     6541 tctactttct ttaaccctgc atctaaagcc actttagcag catctgctgc agccatttgt
     6601 gctgcataag gggtattttt cttagaacct ctaaaaccca ttttaccagc agatgaccaa
     6661 gaaataactt gaccagattt gttggtaaat gaaataataa tgttgttgaa agttgcatta
     6721 atacgaactt ctccggcagc atctacttta actactcttt tttttgctgc tgcctttgca
     6781 ctgttttgtg cttttgctgg ttttgccatt tttaattttt ttaggtaatc gtctgtttct
     6841 attttacgtc tcctgttaag cagcttttgc ccaactatcc aacgtattat ttaaaaaacc
     6901 aaaagctctt aactgtttgt tatgagcttt atagttattt attacttttt aggtgctttc
     6961 tttttacctg ctaccgtttt acgttttcct tttctggtac ggctattggt tttagtacgc
     7021 tgtccacgaa ctggtaatcc ctttctgtgt cttaaaccac gataacaagc aatatctaat
     7081 aaacgtttaa tattcataga tgtttcgcta cgcaaagcac cttctacttt aaactcttca
     7141 ttaataatat tacgaatagc agcttgttca tcatcattcc actgatttac ttttttatca
     7201 aaatcaatgc ctgctttagt taaaatatac tgagcagtag aacgaccaat accatacaca
     7261 taggttagcc ctatttcgcc tcttttattt tttggtaaat ctataccggc aatacgtgcc
     7321 ataatttatt aatttctgat tttaaatttc taatttttat tacccttgtc tttgtttaaa
     7381 acgaggattt tttttgttga taacataaag tttacctcca cgtttaacaa ttttgcaatc
     7441 tgcactgcgt tttttaattg atgctcttac tttcattgct tttaatttaa ctgttatgtt
     7501 atttctaaca gattatataa ctttttattt atacctaaag tttattctgc ccctgcttaa
     7561 atcgtatggg ctcatttcta ctcctacttt atcgccaggc aaaattctga tataatgcat
     7621 tctcattttt ccagaaatgg tagctaaaat ttcgtgtcca ttttctagtt ttactctaaa
     7681 cattgcattg cttaaagcct ctaaaatgat accatcttgt ttaatcagtt gttgcttaga
     7741 catatttttt cgggctgcaa agataggagt atgtacttaa tttcaaaaaa agtttgaaaa
     7801 aaataatttt cctataataa aatcatctta tttatgaaaa atattgtatt aaaaagaaag
     7861 cctttcactt tgaaaagaac gataatacac caatccatta agctaaaaaa atgttttgct
     7921 aatattcttt tttttctctg ctttgagcta tatcttaact aaaaaaaata tatatggata
     7981 aaatacactc caacttaact agctaataat cagcatgata gcttacactt aaaaattgtt
     8041 ttttatttct aaagaaacat tgcacatttg cggcgaattt ggtttatctg cttcaggcag
     8101 atgattaaaa gggaatcatg tgaaaagcat gaactatccc cgtagctgta aagtagcttt
     8161 ctttaacaaa gaaaaatatt tcaaataata gtttgccact gtttt
//
LOCUS       DAOQPK010000084         8108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_4911_length_8108_cov_4.888985, whole genome shotgun sequence.
ACCESSION   DAOQPK010000084 DAOQPK010000000
VERSION     DAOQPK010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8108
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4911_length_8108_cov_4.888985"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="PLU36_05460"
     CDS             <1..1054
                     /locus_tag="PLU36_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HMZ46231.1"
                     /translation="KEIKEFNIDTIEDVNDLKAITEQLIQIPNIASKRWIYNQYDSMV
                     GTANASTNAPSDAAIILAKGTGKALAVTVDCNSRYVFANPFVGAMIAVAEAARNIVCS
                     GGEPIGVTNCLNFGNPYDPEVYYQFVKAVTGMGDACRKFNTPVTGGNVSFYNQSPEGA
                     VYPTPTIGMVGILDNIDNKMTLSFKSEGDIIVLIGEQKNDIASSEYLHKIKKIEYSDV
                     PYFDLDEEFKIQQFIALIINKKLIQSAHDISEGGLIITLMEKGFYNNLGFSISYNTTI
                     RKDAYWFGEAQSRVVVTVANSNYESFKTFITKANIPFTQIGTVTAGEIEINNDKWDNI
                     LVWKEKYDTAIEKHLN"
     gene            1070..2080
                     /gene="rfaD"
                     /locus_tag="PLU36_05465"
     CDS             1070..2080
                     /gene="rfaD"
                     /locus_tag="PLU36_05465"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HMZ46232.1"
                     /translation="MNNINTSTNILITGAAGFIGSCMASFLNSKGYKNIYLVDDFGVE
                     IKRKNWEAIQRINIIERYNLFNWLTKEKPTINFIIHLGARTDTTEFNYEVHRELNYNY
                     SIHVWQYAVQNNIPFVYASSAATYGDGSLGYEDTHEIIQKLQPLNPYGVSKNEFDKWA
                     VKQTVHPPSWAGLKFFNVYGPNEYHKGRMASVIWHSFNQIKNNGVVKLFKSHKDGFED
                     GKQLRDFIYVKDVVLVIFWMMNCMLTNQWNKTKNGLFNLGTGKARTFEDLVKATFSGL
                     DLPSKIEFIDMPEDIRDKYQYFTEANMQKLYDAGYANTFYTLEEGVTDYVKNYLTNLK
                     IY"
     gene            complement(2180..3607)
                     /gene="dnaA"
                     /locus_tag="PLU36_05470"
     CDS             complement(2180..3607)
                     /gene="dnaA"
                     /locus_tag="PLU36_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HMZ46233.1"
                     /translation="MAKTAETVWVNCLKIIKDIVEWQHFKTWFEPINPVELKNNVLMI
                     QVPSQFFYEYLEEHYVALLAKTLKRELGKEARLEYRIMVDSGNGKNGSMDIPTSGVKT
                     YNNNEMNFPLVIDNPVKNPFVIPGLKKMQIDPQLNSKYTFDAFIEGDCNRVARRAGKT
                     VAEKPGANSFNPLVIYGGTGLGKTHLIQAIGNEVKRTNPNKVVLYVSSEKFINQFQDH
                     SRNNAINDFIHFYQLIDVLIIDDVHFFARAEKSQDAFFAIFNHLHQSGKQLVLTSDKA
                     PKDLDGMQERLLSRFRWGLSADLQIPDYETRIEILERKMKNDGLDMPREVVKYVAYNI
                     NTNIRELEGALISLLAQSSLNKKEIDLELAKKVLRNFVKTSSKEITIDAIQKMVCEYF
                     DVPYEKLLHKTRKREIVQARQITMYLAKAFTKNSLKTIGEHFGGRDHTTVIHSCQTVK
                     DLMDTDTIFRENVMELTQKVQLAAM"
     gene            complement(3745..4683)
                     /locus_tag="PLU36_05475"
     CDS             complement(3745..4683)
                     /locus_tag="PLU36_05475"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMZ46234.1"
                     /translation="MHRRKFLKETTLAGSALIASSILPFNSLYAAVKDDTKNISILHT
                     NDVHSRLEPFPMDGSRNAGLGGVAARAALIKDIRQKTENVLLLDAGDIFQGTPYFNLY
                     KGEPEIKAMSLMGYDAATMGNHDFDAGLENFANQLIHANFPIVVCNYDFNNTPMEGKY
                     LPYTIVKKGKLKIGILGVGIEGKGLIPADLFGNTIYNNPIECANNAANKLKKEGCDLI
                     ICLSHLGDKYNDDKISDEILAKETYDIDLIIGGHTHKFFKEPRKYNNRKNKITLVNQV
                     GWAGIQLGRLDYTFSKIKNKNLANSETVVIGGKSSE"
     gene            complement(4694..5428)
                     /locus_tag="PLU36_05480"
     CDS             complement(4694..5428)
                     /locus_tag="PLU36_05480"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HMZ46235.1"
                     /translation="MFKQIFATYFLAILLFACSTPKQNISIQFKDYRIQQSDKKDSTL
                     TAMLKPYSDNIHQSMNTVIGFSTKGLSKKQPESELGNFLADCMKIMAEEKFSQKVDAA
                     FLNYGGIRSYLPKGDITTGNIYELMPFDNLVVLQQVSGKTLNQFLNKIAERGGWPVSG
                     VKMQIKDKQAVNIFINNKPLEESATYTIANNDYIARGGDDCDMLKEIPVNNKGYLFRD
                     AIIAFIKQLTKQGKPIEWKIENRITY"
     gene            5546..5929
                     /locus_tag="PLU36_05485"
     CDS             5546..5929
                     /locus_tag="PLU36_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HMZ46236.1"
                     /translation="MSYEIVGKLVAKFDIVQRTETFKTREFVIEKSEDIGGRIITNYI
                     KFQCVQDKTTMPERFNIGDEVKVLFNIKGTKWNKNGTDNYITNLDAWRMETSKLGTTV
                     ENTTSNTNEFTPTNNTDDLVDDLPF"
     gene            complement(5918..7474)
                     /locus_tag="PLU36_05490"
     CDS             complement(5918..7474)
                     /locus_tag="PLU36_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46237.1"
                     /translation="MNLVKYITNYFSSKNRIFLVCILTIHVIFSYYFISKQYITYDEP
                     SYIEYSKRWLHGKPERVEALDDSKTPVISIVWLPRIVQQIIQPNLKQTDFGKKDQEQG
                     RYMMIIFSLLLLIYFYKFLQLLKIKYWLPVFLFIIIDPLLIAYSVLINSDLLSGLVLL
                     AIIYHLYQYLFFKKRSHFIYSCIWLATGLVTKLSFVFFIPVFWLIIFVVHKKIDWRKF
                     LLFVLLILLVVNTWFYFKNTCLPLGQFQFSSNTFKLVQQFLHFLHWLPLPLPENYIYS
                     IDLLQYHAQLGGSYNNTYPGVYILNKFSLTNNFWYYYLVAAWYKFPISILFLITTGLF
                     AFLYRFKKNIQKYWIIIISIVYYLLVLSCFNKFQIGIRHLLIVLPLMYILIAVLLNYL
                     VQTKFKWGIAILWLFMFFSVAKYYPNLIPYTNELVTNKAKVYEKLMDTSIDYGQSKLD
                     ASNFLQQNTDFKTLTETPQTGKYLVSMQQLVLHQMYKDTSYNWLIKNYEPIGMYKYVY
                     LQFKIDALKR"
     gene            complement(7477..>8108)
                     /gene="truB"
                     /locus_tag="PLU36_05495"
     CDS             complement(7477..>8108)
                     /gene="truB"
                     /locus_tag="PLU36_05495"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HMZ46238.1"
                     /translation="FDVVKKIRSLIKVSKIGHAGTLDPLATGLLIICTGKFTKKINEF
                     MGQPKTYTGSFTLGATTSSYDLEQTPENFKDISHLTATEIYNATLPFIGNILQMPPQH
                     SAIKKDGKRLYEMARKGIEVKVEPRPVTIYTFEITKIELPLVYFKVICSTGTYIRSLA
                     NDFGNALNTGAYLSSLCRTDIGEFNIENAYTIESFENEIAKLRNSETTI"
BASE COUNT         2858 a         1182 c         1163 g         2905 t
ORIGIN      
        1 aaaagaaatt aaagaattta atattgatac aattgaggat gtaaatgatt taaaagccat
       61 aaccgaacaa cttattcaaa ttcccaatat agcttctaaa cgttggattt acaatcagta
      121 cgatagtatg gtaggtactg caaatgcaag tacaaatgca cctagcgatg cagcaattat
      181 tttagcaaaa ggcacaggaa aggcattagc tgtaactgtt gattgcaata gccgatatgt
      241 atttgccaat ccatttgttg gtgcaatgat tgctgtggca gaagctgcaa gaaatattgt
      301 ttgtagcgga ggagaaccca ttggtgtaac taattgctta aattttggta atccgtatga
      361 cccggaagtt tattatcagt ttgtaaaagc tgttacaggc atgggcgatg cttgtagaaa
      421 atttaatacc ccagtaacag gtggcaatgt tagtttttat aatcaaagcc ccgaaggtgc
      481 agtttaccca acaccaacta ttggaatggt aggtatttta gataatatag ataataaaat
      541 gaccttgagt tttaaaagtg aaggtgatat tattgtatta attggagaac aaaaaaatga
      601 tattgcatct agcgaatatt tgcataaaat taaaaagata gaatattctg atgtaccata
      661 ttttgactta gacgaggaat ttaaaataca acaattcatt gcattaatca tcaataaaaa
      721 attaatacaa tctgcacacg atataagcga aggtgggttg ataataacct taatggaaaa
      781 aggcttttat aataatttag gctttagtat ttcttataat actactattc gtaaagatgc
      841 ttattggttt ggagaagcac aaagtagggt agtggttact gttgcaaatt ctaattatga
      901 aagttttaaa acattcatta caaaagcaaa tattccattt acacaaatag gaacagtaac
      961 agccggagaa attgaaataa ataacgataa atgggataat attttggttt ggaaagagaa
     1021 gtatgataca gccattgaaa agcatttaaa ctaattaata ttttattaaa tgaataatat
     1081 aaacacttca accaatattt taattactgg tgctgcaggt tttattggta gttgtatggc
     1141 ttctttttta aatagtaaag ggtataaaaa tatttatttg gtagatgact ttggagtaga
     1201 aataaaaaga aaaaactggg aagccattca aagaattaat ataattgaaa ggtataattt
     1261 atttaattgg cttactaaag aaaagcccac cataaacttt ataatacatt taggagccag
     1321 aacagatacc acagaattta attacgaagt tcaccgagaa ttaaattata attactccat
     1381 tcatgtgtgg caatatgcag tacaaaataa tattccattt gtttatgcat cttctgctgc
     1441 tacctatggc gatggtagtt taggatatga agatacccat gaaataattc aaaaattgca
     1501 accattaaat ccttatggtg ttagtaaaaa cgaatttgat aaatgggcag tcaaacaaac
     1561 cgttcatcca ccaagctggg ctggcttaaa attttttaat gtttacggac ctaatgaata
     1621 ccataaagga agaatggcaa gtgtaatttg gcattcattt aatcaaatta aaaataatgg
     1681 tgtagtaaaa ctatttaaaa gccataaaga tggttttgaa gatggaaaac agctaagaga
     1741 ttttatttat gtaaaagatg ttgttctggt aattttttgg atgatgaact gcatgcttac
     1801 taaccaatgg aataaaacaa aaaatgggtt atttaattta ggtacaggta aagccagaac
     1861 atttgaagat ttggttaaag caactttttc tggcttagat ttaccaagca aaatagaatt
     1921 tattgatatg ccagaagata taagagataa gtatcaatat tttactgaag ctaatatgca
     1981 aaaactgtat gatgcaggct atgcaaatac tttttatact ttagaagaag gcgtaacaga
     2041 ctatgtaaaa aattatttaa ctaatttaaa aatatattaa ctgcttgaat taagatttat
     2101 aactacaccc ttttaacaaa actgcttctt cattgtattt tgaagaagca gttttgatta
     2161 attaaatagt ataataaaac tacatagctg caagctgtac tttttgagta agctccatta
     2221 cgttttctct aaaaatggta tcagtatcca ttaaatcctt caccgtttgg caactatgaa
     2281 ttacagtagt gtggtctctt cctccaaaat gctcaccaat tgtttttaaa gaatttttag
     2341 taaatgcttt tgctaagtac atggttattt gccttgcttg aactatttct ctttttcttg
     2401 ttttatgtaa tagtttttcg tatggtacat caaaatattc gcaaaccatt ttttgaatag
     2461 catcaatagt aatttcttta ctacttgttt taacaaagtt tcttaatact tttttagcaa
     2521 gttctaaatc tatctctttt ttgtttaaag aagattgagc taaaagagaa attaaagcac
     2581 cttctaattc acgaatattg gtattaatat tataagcaac atattttaca acttctctag
     2641 gcatatctaa gccatcattc ttcattttac gttcaagaat ttcaattctt gtttcataat
     2701 ctggtatttg caaatcggca cttaagcccc aacgaaaacg gcttaataac ctttcttgca
     2761 taccatctaa atctttagga gctttatcgc tggttagtac caattgtttt ccactttgat
     2821 gtaagtggtt gaagattgca aagaaagcat cctggctctt ttcagctctt gcaaaaaagt
     2881 gaacatcatc aataattaaa acatctatta attgataaaa atgtataaaa tcattgatag
     2941 cattgtttct gctatggtct tgaaattgat taataaactt ttcgctgctt acataaagaa
     3001 ctactttatt tggattagtt ctttttactt cattacctat tgcttgtatt aaatgagttt
     3061 ttcctaaacc agttccacca taaataacta atggattaaa agaattggct cctggctttt
     3121 ctgcaactgt ttttcctgct cttctggcaa ccctgttaca atctccttct ataaatgcat
     3181 caaatgtata tttagaattt aattgtggat ctatttgcat tttctttaag cccggaatta
     3241 caaatggatt ttttactgga ttatcaataa caagcggaaa attcatttcg ttattattgt
     3301 atgtttttac accacttgta ggtatatcca tacttccatt tttgccatta ccgctatcta
     3361 ccattattct atattccaat cttgcttcct tacctaattc tctttttaaa gtttttgcaa
     3421 gtaaagcaac ataatgttct tctaggtact cataaaagaa ctggctgggt acctgaatca
     3481 ttaaaacatt gttctttagt tctaccggat taattggctc gaaccaagtt ttaaaatgtt
     3541 gccattctac aatgtcttta ataattttaa ggcaatttac ccatacagtt tctgcagttt
     3601 tagccataat tagtttacaa attatataat tagttaagaa tattttttta gctccagttt
     3661 atcaaaattt tcaaatgtta ataagttgaa aacttttttt tgaagggagt gcaatatgtg
     3721 taataaatat tgaaaaaaaa atttttattc actacttttt ccccctatta caacagtttc
     3781 tgaattagcc aaatttttat tttttatttt gctaaaagta taatccaatc tacctaattg
     3841 aataccagcc caaccaacct gatttactaa tgttatttta ttttttctat tattatattt
     3901 tcttggttct ttaaaaaatt tatgtgtatg cccgccaata attaaatcta tatcgtaggt
     3961 ttcttttgct aaaatttcat cgcttatttt atcatcgttg tatttatctc ctaaatgcga
     4021 taaacaaata attaaatcgc aaccttcttt ttttaattta tttgctgcat tgttagcaca
     4081 ttcaattggg ttgttataaa tggtattgcc aaataaatcg gcaggtataa gtccttttcc
     4141 ttctattcca actccaagta ttcctatttt taatttacct tttttaacta tagtgtaagg
     4201 taaatatttc ccttccattg gggtattatt aaaatcataa ttacaaacaa ctatcggaaa
     4261 atttgcatgt attaactgat tggcaaaatt ttctaaacct gcatcaaaat catgattacc
     4321 catagtggca gcatcatagc ccattaaact cattgcttta atttctggtt cgcctttata
     4381 caaattaaaa tatggagttc cttgaaaaat atctcctgca tctaacaata aaacattttc
     4441 tgttttttgt cgaatatctt ttattaaagc agctctagca gctacacctc ctaagccagc
     4501 atttctacta ccatccatcg gaaaaggttc taacctgctg tgaacatcgt tggtatgtag
     4561 gatagaaata ttttttgtat cgtctttaac agctgcatac aaactattaa atggtagtat
     4621 agaggaagct attaaagcac taccagctaa tgttgtttct tttaaaaatt ttctacggtg
     4681 cattgttatt tttttaataa gttattctgt tttctatttt ccattctatt ggtttgcctt
     4741 gtttggttaa ctgtttaata aaagcaataa tggcatctct aaataaatac cctttattgt
     4801 taaccggaat ttcttttagc atatcgcaat catctccacc acgtgcaata taatcgttat
     4861 ttgcaatagt gtaagtggca ctttcttcta atggtttatt attgatgaaa atatttacag
     4921 cttgtttatc ttttatctgc attttaacac cacttacagg ccaaccacca cgttcagcaa
     4981 ttttatttaa aaattggttt aatgtttttc cgcttacttg ttgcaataca actaagttat
     5041 caaaaggcat taattcgtaa atatttcctg tagtaatatc tcctttaggt aaataagatc
     5101 ttatgccacc atagtttaga aatgctgcat ctactttttg agaaaatttt tcttcagcca
     5161 ttattttcat gcaatctgct aaaaaatttc ctaattcact ttctggttgt tttttagata
     5221 agccttttgt agaaaaacct attaccgtat tcatagattg atgaatatta tccgaataag
     5281 gtttaagcat tgctgttaat gtgctgtctt ttttatcgga ttgttgtatt ctataatctt
     5341 taaattgaat ggatatattt tgctttggag tggaacaggc aaataataat atagctaaaa
     5401 aataggtagc aaaaatttgt ttaaacatgg tgaaatttta taaaaaatga aagttagatt
     5461 tttgctgtta ataaaacaaa gtttttttat gaataaagca aaatttatta agctaccttt
     5521 gtaattaata aaaaatatta tttatatgag ttatgaaatt gtaggaaaat tagttgcaaa
     5581 atttgatatt gttcaacgta cagaaacttt taaaactaga gagtttgtaa ttgaaaaatc
     5641 ggaagacatt ggtggtagaa ttattacaaa ctatataaaa tttcaatgtg tacaagataa
     5701 aactactatg ccagaaagat ttaatattgg tgatgaagta aaagtattat ttaatattaa
     5761 aggaactaaa tggaataaga atggtactga taattatata accaatttag atgcatggag
     5821 aatggaaaca agtaaacttg gtactactgt agaaaataca acaagcaata ccaacgaatt
     5881 tacaccaact aataataccg atgatttggt agatgattta cctttttaat gcatctattt
     5941 taaattgtaa ataaacatat ttatacatcc caattggctc ataatttttt atgagccaat
     6001 tgtatgaagt gtccttatac atttggtgca aaacaagttg ttgcatactt actaaatact
     6061 ttcctgtttg tggtgtttct gttagtgttt taaaatctgt gttttgttgc aaaaaattac
     6121 ttgcatctaa tttgctttgc ccataatcaa tacttgtatc cattaatttc tcatatactt
     6181 tggctttatt ggtaaccaat tcattagtat atggaattaa gtttgggtaa tattttgcaa
     6241 cagaaaaaaa cataaaaagc cacaatattg caatacccca cttgaacttt gtttgcacta
     6301 agtaatttaa taatactgca attaaaatat acattaaagg taaaacaatt agcaaatgcc
     6361 ttataccaat ttgaaattta ttaaagcaac ttaatactaa taaatagtaa acaatagaga
     6421 taataattat ccaatacttt tgaatatttt ttttaaacct ataaagaaaa gcaaataaac
     6481 cagttgttat taaaaataaa atactaatag gaaatttata ccaagctgcc actaaataat
     6541 aataccaaaa attatttgta agtgaaaatt tgttgagtat ataaacacct ggatatgtgt
     6601 tgttgtaact acctcctaat tgtgcatggt attgcaacaa atctatacta taaatataat
     6661 tttcgggcag tggtaaaggt agccaatgca aaaagtgtaa aaactgttga actaatttaa
     6721 aagtattgct gctaaattga aactgaccta aaggtaaaca agtgttttta aaataaaacc
     6781 aagtattaac aacaagtaaa ataagtaaaa caaaaagtaa aaattttctc caatctattt
     6841 ttttatgaac tacaaatatt attagccaaa atacaggaat aaaaaaaaca aagctcaatt
     6901 tagttaccaa gcctgtagct aaccaaatac aactataaat aaaatggctt ctttttttaa
     6961 aaaataaata ttggtacaaa tgatatataa tagcaagtaa aactaatcca cttaataaat
     7021 cggagtttat taatacagaa tatgcaataa gcaggggatc aataataata aataaaaaaa
     7081 caggtagcca atattttatt ttaagaagtt gaagaaattt ataaaaataa ataagtagca
     7141 gtagcgaaaa tattatcatc atgtacctgc cttgttcctg atctttttta ccaaaatctg
     7201 tttgctttaa gttaggttga ataatttgct gaacaatacg tggcagccat acaatactaa
     7261 tcactggtgt tttactatca tctaaagctt ctactctttc tggtttaccg tgtaaccatc
     7321 gtttactata ctcaatataa cttggttcat cataagtaat atattgtttg cttataaagt
     7381 aataagaaaa gataacatga atagtaagaa tacaaaccaa aaaaattctg tttttactcg
     7441 aaaaataatt ggttatatat tttaccaaat tcatatttaa atggttgttt cagaatttct
     7501 aagctttgct atttcatttt caaaactttc aattgtgtag gcattttcaa tattaaattc
     7561 acctatatct gttctacaca aactacttaa ataagcacca gtatttaaag cattaccaaa
     7621 gtcattcgca aggcttctta tataagtgcc agtactacaa ataactttaa aatacaccaa
     7681 tggcagttct atttttgtaa tttcaaatgt ataaattgta acaggtcttg gttctacctt
     7741 tacttcaatt ccctttcggg ccatttcgta taatctttta ccatcttttt taattgcaga
     7801 gtgctgtggt ggcatttgta aaatattacc aataaatggt aaagtggcat tataaatttc
     7861 tgttgcagtt aagtgagaaa tatctttaaa attttctggt gtttgctcta aatcataaga
     7921 gcttgtagta gcacctaaag taaaactacc agtgtaggtt ttaggctgcc ccataaattc
     7981 atttattttt tttgtaaatt ttcctgtaca aataattaaa agccctgtag ctaaagggtc
     8041 taaagtgcct gcatgaccaa ttttagatac tttaataagt gaacgaattt tttttactac
     8101 atcaaaac
//
LOCUS       DAOQPK010000085         8052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_4962_length_8052_cov_4.912967, whole genome shotgun sequence.
ACCESSION   DAOQPK010000085 DAOQPK010000000
VERSION     DAOQPK010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 8052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8052
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4962_length_8052_cov_4.912967"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLU36_05500"
     CDS             complement(<1..183)
                     /locus_tag="PLU36_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HMZ46239.1"
                     /translation="MKQYKHLFFDLDHTLWDLEANAKETLHELYVKYNLLQIGIVDFD
                     LFFNKYSIHNERLWNRY"
     gene            290..988
                     /locus_tag="PLU36_05505"
     CDS             290..988
                     /locus_tag="PLU36_05505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ46240.1"
                     /translation="MNIIITGASKGLGKAFAKIFAAQNHTLLLCARNQQQLTETATEI
                     KEEFPLATIHYTTKDLSKKNDAIEFGNWCLQFGKPSILINNAGQFIPGSIYNEEDGIL
                     EKMIEINLYSAYHLTRAILPSMIKNKEGHIFNICSIASLKAYTNGGSYSISKYALMGF
                     SKNLREELKPFQVKVTTVYPGAVYTDSWSNTGVEENRIMEPNDIAKMVYSASLLSPQA
                     CVEDIVLRPQLGDI"
     gene            complement(993..1562)
                     /locus_tag="PLU36_05510"
     CDS             complement(993..1562)
                     /locus_tag="PLU36_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46241.1"
                     /translation="MKNLAFILVLFISLSATSQVNKFDSTMKNGKVGYRVICKNKLNN
                     KNSLRVKLIGFEETKDEIDIDIKGKVVGAEVDDLNKDGYPDLVVYIITDDAKRKGTVF
                     AIASNENKGILPILFPSISENEKLKVGYDGNDQFQLINGVLSHKFAVADSTATKEQYA
                     VARQVLYNVIKIETAWKFTVLRSIEIPRP"
     gene            1713..2258
                     /locus_tag="PLU36_05515"
     CDS             1713..2258
                     /locus_tag="PLU36_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ46242.1"
                     /translation="MHLDDVTLLQQFKQPALKEKAFTALVKKYQEKIYWHIRRLVVTH
                     DDANDVLQNMFIKVWNGLENFREESKLYTWLYRIATNESLTFLDSQKKKAVVGFSEVE
                     EGLSNKVKAEVNFDSNKLEWKLQLAIQQLPEKQRAVFTLRYYDEMPYEEMSKILETSE
                     GALKASYHHAAKKIQDFILDN"
     gene            2291..2971
                     /locus_tag="PLU36_05520"
     CDS             2291..2971
                     /locus_tag="PLU36_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46243.1"
                     /translation="MSNLNNIQDELLQVAPNIINLADKNVYTVPNNYFNCLSDVILIK
                     IKSSSLSSKSAKSNHPYLAPDNYFDEFSNKIASIASAQNRHFSEIEYELNEVAPLLNS
                     INKNNIYTTPTNYFEQNKINPKQTNSLKSKWWLYAAAAVFFGITCIGIIKYINTSNKQ
                     VNLSTELAKANDEELTDFLDNQSKPIFYTHQTDEDEFGIFEGTETDELIYFLNNQPQK
                     GETLIKGI"
     gene            2986..3396
                     /locus_tag="PLU36_05525"
     CDS             2986..3396
                     /locus_tag="PLU36_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46244.1"
                     /translation="MKQILILFTFVALSFSSLAQGNKRGEKVQALKVAYMTQVLKLTP
                     QEAQSFWPVYNTYFEESKKARMNNTNDELKYQEEALNIKKKYKPEFKKILNDESRANL
                     VFKAERDFLVELQKEVKKRIQQRQKNKMKHNEFE"
     gene            complement(3486..5309)
                     /locus_tag="PLU36_05530"
     CDS             complement(3486..5309)
                     /locus_tag="PLU36_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HMZ46245.1"
                     /translation="MKIFFRLLQFSRPYHHYIPEYIVYIILYIIFGLVNFSLLAPLLD
                     VLFNNNLPVIDTAPTFSFSGAYFKDYFYFILNKYIQSNGKFGVLVYVCAIIFISILLK
                     NVFGYLGQRVLTRMRVNLLRKLRERLFFQYSNQSLSFFHKEKKGDLLSTISNDVVEIE
                     NSVVNSIQTIFRDPFIIIATFVMLFYLSYQLTLFTLIVFPISGFVISNITQKLRKKAN
                     FIQYLTGVILNIAEEAISGIRIIKGFGAEKTAQQKFDKANYSLTRTIKSIINQRELAS
                     PISEVLGVTVVLVIIVYGGHLILNGESSITASGFIAYIAFYFQIIAPAKNTVNAFANM
                     QRGLSAGERVLRIIDAKQEIIDTENAVAKNDLTQKIEFKNVSFAYNENHVLQNINFEV
                     IKGKTVALVGESGAGKSTIADLIPRFYNVSSGEILIDGINIKDIKIKDVRQLMAIVSQ
                     EAILFNDTVLNNITFGNEISNLDSAQEAAKIANAHDFILQLEDGYNTNIGDRGSRLSG
                     GQKQRLTIARAIYKNAPILILDEATSALDTESEKLVQEAIDSMMKNRTCIIIAHRLST
                     IKHADEIIVLQKGAIVERGRHEDLIANNGIYSNLVKMQELV"
     gene            complement(5368..6147)
                     /locus_tag="PLU36_05535"
     CDS             complement(5368..6147)
                     /locus_tag="PLU36_05535"
                     /inference="COORDINATES: protein motif:HMM:NF013882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HMZ46246.1"
                     /translation="MMINQQQEFFNHFNTFFNHIYVLTIERAKERQEAISKEFEGLNF
                     NFFYGFDKKNLDNMELEQNNIYSSSKAIKNSRYNKPMKLGEIACSMGHKAIYEDMIAN
                     NYSNALILEDDVSINNEPGINLENIFKQLPKNWDLVYFDYFKNDKATLVGTTKQLVYH
                     LQKKLGFLKWSHTTISNLYAKPFSANLKIAGYHDFTSAYAINLKTAKLLIEKQTPICN
                     PADHALSYIITNKLINAYITVPKIFFQKSQTNKEMYNSYVE"
     gene            6192..7334
                     /locus_tag="PLU36_05540"
     CDS             6192..7334
                     /locus_tag="PLU36_05540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMZ46247.1"
                     /translation="MYSILFISLMNSDPWGGSEVQWYATANYAIDRGDKVTCMVYEWD
                     AKKEKMELLKKRGATIIYIPNFGRAKRNLYERLSFEWVTRGKQRRFINNFIFSSFDFA
                     VVNQGGFMEVANNPWKNLYKKLPPYIVTFHNYTLNYSFKPAKAKTLTAWMLNAKCLIF
                     AAAKAISELEKQLNNSFKNVLTLASPLSIEKSLNYTPYPSLKNGKYKVIMLAHLDVNR
                     KAQDNLIKAFANDVWKKRNVILEIYGGGEHFNMLQELITKLNLQEIVFLKGNTNNVAE
                     VLNTAHLVLQITHKDAMPISVIEAMSKSRAVVVSNVGDMPVWIKDNETGWVTPDASIQ
                     SIEQGLETAWQNKTQWEQMGKNAFDFFNKNFPSSVEEVFYTTLIKK"
     gene            7338..>8052
                     /locus_tag="PLU36_05545"
     CDS             7338..>8052
                     /locus_tag="PLU36_05545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HMZ46248.1"
                     /translation="MKLFKKKNKEENLITPEFKEFEEIYNLCKPYTMTGIERMFALYK
                     ATEYIVKNNIEGDFVECGVWRGGSSMVILKTLQKFNVTNRKIWLFDTYEGMSEPTEDD
                     KDFTNLSADKQLQKSKKDADSFIWAYATLEDVKKNIELTKYPLENVHFIKGKVEDTIP
                     EKNYFNHCSLLRLDTDWYESTKIELEYFYPLLQKNGVMIIDDYGHWQGCKKAVDEYFA
                     TQNFCPLLQRIDYTGRIMTK"
BASE COUNT         2990 a         1197 c         1145 g         2720 t
ORIGIN      
        1 atatctattc cacaaccttt cattatgaat actgtatttg ttaaaaaaca aatcaaaatc
       61 tacaatacca atttgcaata aattatattt tacataaagt tcgtgtaaag tttcttttgc
      121 attggcttct aaatcccaca aggtatgatc aaggtcaaaa aacaaatgtt tgtactgctt
      181 cataattgca aataaatgtt attgtattta tattttagac tgtatataaa taaactttta
      241 aacagttttt cttaaaaaaa tttgtttctt cgtgaagaaa taatttatca tgaatattat
      301 tataacaggt gcctctaaag ggcttggaaa agcatttgca aaaatatttg ctgctcaaaa
      361 tcatacttta ttattatgtg ccagaaacca acaacaactt acagaaactg ctacagaaat
      421 taaggaagag tttcctttag caaccattca ttatactaca aaagatttaa gtaaaaaaaa
      481 cgatgctatt gaatttggta attggtgttt gcaatttgga aaaccctcta tactaataaa
      541 taatgctgga caatttatcc ctggttctat ttataatgaa gaagatggta ttttggaaaa
      601 aatgatagaa attaatttat atagtgccta ccatttaact agagcaattc taccttctat
      661 gattaaaaat aaagaaggac acattttcaa tatttgctcc atagcttcct taaaagctta
      721 tacaaatggt ggtagttata gcattagtaa atatgcatta atgggcttta gtaaaaattt
      781 aagagaagag ttaaagcctt ttcaagtaaa agttacaaca gtttaccctg gtgcagtata
      841 tacagatagt tggtctaata ctggtgtgga agaaaataga attatggagc ctaacgacat
      901 tgctaaaatg gtatattctg cttctttatt atctcctcaa gcttgtgtgg aagatattgt
      961 tttaaggcct caattaggag atatttaatt tattaaggtc tgggtatttc tatacttctt
     1021 aatactgtaa atttccaggc agtttctatt ttaattacat tgtataaaac ctgtcttgca
     1081 acagcatatt gctctttagt agcagtagaa tctgcaactg caaatttatg tgagagcact
     1141 ccattaatta actgaaactg atcattgcca tcgtatccta cttttagttt ttcattttcc
     1201 gaaatgcttg gaaatagaat gggtaaaata cctttgtttt cgttagatgc tattgcaaaa
     1261 acagttcctt ttcttttggc atcatcggta ataatgtaaa ccactaaatc aggataacca
     1321 tctttattta aatcatcaac ttcggcacct acaactttcc ctttaatatc aatatctatt
     1381 tcgtctttag tttcttcaaa ccctattaat ttaaccctta aactgttttt attgttcaat
     1441 ttatttttac aaattactct ataacccact ttcccatttt tcattgtact atcaaactta
     1501 ttaacctggc ttgttgcact taagctaata aataatacta aaataaaagc taaattcttc
     1561 ataattaaag taaatcttta gtgtaatacg gcaaatgtaa ctaatagctt taaaaaataa
     1621 caggatacta attttaaaat tttataaaaa catatactaa ttttaaacta atgtaaaaat
     1681 aaaaagtctt acaacgcaca aacaccattt tcatgcattt agacgatgta accttattac
     1741 aacagtttaa acaacctgct ttaaaagaaa aagcatttac cgctttagta aaaaaatatc
     1801 aggaaaaaat atattggcat ataaggaggc tagttgttac acatgatgat gctaatgatg
     1861 tgttacaaaa tatgtttata aaagtatgga acgggttaga aaattttaga gaagaaagta
     1921 agctttatac ctggttgtat agaatagcca ccaacgaaag tttaacattt ttagacagtc
     1981 aaaaaaagaa agcagtagtt ggctttagcg aagtagaaga agggttaagc aataaagtaa
     2041 aagctgaagt aaattttgat tctaataaat tagaatggaa gctacaactt gccatacaac
     2101 aattgccaga aaaacaaagg gctgttttta cgctacgtta ttacgatgaa atgccctacg
     2161 aagaaatgag taaaatactg gaaaccagcg agggtgcttt aaaagcaagc taccatcatg
     2221 cagcaaaaaa aattcaagac tttatattag ataattaaac tttatacccc aaaaatggtc
     2281 taactaaaca atgagcaatt taaataacat acaagatgaa ttattacaag ttgcacccaa
     2341 tattattaat ttggctgaca aaaatgtgta cactgtaccc aataattatt ttaattgttt
     2401 gtctgatgtt attttaataa aaataaagtc ttcttctctt tcatcaaaaa gtgccaaatc
     2461 caatcatcca tatttagccc ctgataatta ttttgatgaa ttttcgaata aaatagcctc
     2521 cattgcatct gcccaaaaca gacatttttc tgaaattgag tatgaattga atgaggtagc
     2581 tcctttatta aattcaatta ataaaaataa tatttataca acccctacta actattttga
     2641 acaaaataaa ataaacccaa aacaaaccaa ttctcttaaa agtaaatggt ggttgtatgc
     2701 agctgcagca gtattttttg gtattacttg tataggaatt attaagtata ttaatactag
     2761 taacaagcaa gttaacctaa gtaccgagct tgcaaaagcc aatgatgaag aacttacaga
     2821 ttttttagac aatcaatcta aacctatttt ttacacacat caaaccgatg aagatgagtt
     2881 tggaattttt gaaggaaccg agacagatga attaatttat tttttaaaca accaacccca
     2941 aaagggcgaa actttaatta aaggcatata aatcaataac ttactatgaa acagatactt
     3001 attttattca cctttgtagc actttcattt agttctttgg cacaaggcaa caaaagagga
     3061 gaaaaagttc aggctttaaa agtagcttat atgacacaag tacttaaact tacaccacaa
     3121 gaagctcaaa gcttttggcc agtttataat acttattttg aagagtctaa aaaagccaga
     3181 atgaacaata caaacgatga attaaagtat caggaagaag cattaaatat taaaaagaaa
     3241 tataaacctg aatttaaaaa aattttgaat gatgaaagcc gtgccaacct tgtttttaaa
     3301 gctgagagag attttttagt tgaattacaa aaagaagtaa aaaaacgaat tcagcaaaga
     3361 caaaaaaaca aaatgaaaca caacgaattt gagtaaaagt ttaatttata tactttacct
     3421 tatttaactc tggtgttttt cataggttag caacggccag ctctttttag agcaggcctc
     3481 cttttttaaa ccaactcttg catttttacc aaattactat atattccatt attggcaatt
     3541 aaatcttcat gtcttcctct ttctacaatt gctccttttt gcaaaacaat aatttcatcg
     3601 gcatgtttaa ttgtactaag tctatgggct ataataatgc aagttctatt tttcatcata
     3661 ctatcaatag cttcttgaac cagtttttcg ctttcggtat ctaatgcact tgttgcctca
     3721 tctaatatta aaattggtgc atttttataa atagctctgg caatagttag tctttgcttt
     3781 tggccaccac ttaaccttga ccctctatcg ccaatattag tattgtagcc atcctctaac
     3841 tgtaaaataa aatcatgagc attggcaatt ttagcagctt cttgtgcaga atctaaatta
     3901 ctaatttcgt taccaaaagt tatattgttt aaaacagtat cgttaaataa aattgcttct
     3961 tgagatacaa ttgccatcaa ttgccttaca tcttttattt taatatcctt tatatttatt
     4021 ccatcaatca aaatttctcc actacttaca ttataaaatc ttggaattaa atctgcaata
     4081 gttgattttc ctgctccact ttcacctacc aaagcaacag ttttaccttt aattacttca
     4141 aaatttatat tttgtaaaac atggttttca ttataagcaa acgatacatt tttgaactca
     4201 attttttgag ttaggtcatt tttagctaca gcattttctg tatcaataat ttcttgttta
     4261 gcatcaataa ttctcaatac tctttctccg gcagataagc ctctttgcat attagcaaaa
     4321 gcatttactg tattttttgc aggtgcaatt atttgaaaat aaaatgctat atacgcaata
     4381 aagccagagg ctgtaatact actttctcca ttcaaaatta aatgcccacc atatacaatt
     4441 attactaaaa ctactgttac ccccaacact tcagaaattg gcgaagccaa ctctctttgg
     4501 ttaattatag attttattgt tcttgttaaa gagtaattgg ctttatcaaa tttttgttgt
     4561 gccgtttttt cagcaccaaa gcctttaata attctaatac ccgaaatagc ttcttcggct
     4621 atatttaaaa taacccctgt taagtactga ataaagtttg cttttttgcg tagtttttgg
     4681 gtaatattgg aaataacaaa accagaaatt ggaaaaacta ttaaagtaaa taaagtaagt
     4741 tgatacgata aataaaataa cattacaaaa gtagcaatga taataaaggg gtccctaaaa
     4801 atggtttgaa tagagtttac aaccgagttc tctatttcta ccacatcgtt actaatagtt
     4861 gaaagtaaat cgcctttttt ttctttatga aaaaatgata aagattgatt gctgtattga
     4921 aaaaataatc tctctcttag ttttcttaat aggtttaccc tcatacgagt taatacacgc
     4981 tgccctaagt aaccaaaaac attttttaat aaaatggaaa taaaaataat agcacacaca
     5041 taaactaata caccaaactt gccattgctt tgaatgtatt tatttaaaat aaaataaaaa
     5101 taatctttaa aataagctcc gctgaaagaa aaagttgggg cagtatctat aactggcaaa
     5161 ttattattaa acaacacatc taataatgga gctaataaag aaaagtttac caacccaaaa
     5221 atgatatata aaataatata cacaatatat tctggaatat aatgatgata aggtcttgaa
     5281 aactgaagta gtctaaaaaa aattttcatt tataaaataa atttaatgca aattaaaata
     5341 ttaaagttgg tagataattt tctctcatta ctctacatag ctattataca tttctttatt
     5401 agtttggctt ttttgaaaaa atatttttgg aacagtaata tatgcattta ttaatttatt
     5461 agttataata taagataacg catgatcagc aggattgcaa ataggtgttt gtttttcaat
     5521 taataatttt gcagttttta aattaatagc atatgccgaa gtaaaatcat gatagcctgc
     5581 aatttttaaa ttagcagaaa agggttttgc atataaatta cttattgttg tgtgagacca
     5641 ttttaagaat cctagttttt tttgtaaatg atatactagt tgctttgttg tacctactaa
     5701 agtagcctta tcgtttttga agtaatcaaa ataaaccaag tcccaatttt tgggtagctg
     5761 tttaaaaata ttttctaaat taataccagg ttcattattt atagaaacat cgtcttctaa
     5821 tatcaaagca ttagaataat tgtttgctat catatcttca taaatagctt tgtgtcccat
     5881 actgcaagca atttctccta atttcattgg tttgttatat ctactattct ttattgcttt
     5941 agagctacta taaatattgt tttgctctaa ttccatatta tctaaatttt ttttatcaaa
     6001 tccataaaaa aaattaaagt ttagtccttc aaattctttt gatatagcct cttgcctttc
     6061 tttagccctt tctattgtaa gaacataaat atgattaaag aatgtattaa aatggttaaa
     6121 gaattcttgt tgttggttaa tcatcataat tatctttctt tgtaataagt tttcaaaatt
     6181 aagttactct catgtattca atccttttta tttccttaat gaactcagac ccatggggag
     6241 gtagcgaagt gcagtggtat gcaacagcta attacgcaat tgatagagga gataaagtta
     6301 cttgtatggt atatgagtgg gatgctaaaa aagaaaaaat ggagctattg aaaaaaagag
     6361 gagcaaccat tatttatatc cccaattttg gaagagcaaa acgtaactta tatgaaaggc
     6421 taagctttga atgggttaca agaggcaaac aaagaaggtt tattaataat tttatttttt
     6481 cttcttttga ttttgcagta gtaaaccaag gtggctttat ggaggttgcc aataaccctt
     6541 ggaaaaattt atataaaaaa ttgccgcctt atattgtaac ttttcacaac tatactttaa
     6601 attacagttt taagcctgca aaagccaaaa cgcttacagc atggatgcta aatgcaaaat
     6661 gccttatttt cgctgctgca aaagctattt cagaattaga gaaacaactt aataatagtt
     6721 ttaaaaatgt attaacattg gctagtcctt taagtattga aaaatcttta aactataccc
     6781 cttacccatc cttaaaaaat ggcaagtata aagtaatcat gcttgcacat ttagatgtaa
     6841 atagaaaagc acaagataat ttaattaaag cctttgcaaa tgatgtttgg aaaaaaagaa
     6901 atgttattct tgaaatttat ggtggagggg agcattttaa tatgctacaa gaattaataa
     6961 ctaagttaaa tttacaagaa atagtttttt taaaaggcaa taccaacaat gtagcagaag
     7021 tgttaaatac tgctcactta gttttacaaa taacccataa agatgcaatg ccaataagtg
     7081 taatagaagc tatgagtaaa agtagagcag tagttgtaag caatgttggt gatatgccag
     7141 tatggataaa agataatgaa acaggctggg taacgccaga cgcaagcata caaagcatag
     7201 aacaaggttt agaaactgct tggcaaaaca aaacgcaatg ggaacaaatg ggcaagaatg
     7261 cgtttgactt ttttaataaa aattttccat catcggttga agaagttttt tatacaaccc
     7321 taatcaaaaa ataatttatg aaacttttca agaaaaaaaa taaagaagaa aatttaatta
     7381 caccagagtt taaagagttt gaagaaatat ataatttatg taagccttat accatgactg
     7441 gtatagaaag aatgtttgct ctatacaaag ccactgagta tattgttaaa aataatattg
     7501 aaggcgactt tgtagaatgt ggtgtttggc gtggaggaag ctctatggta attttaaaaa
     7561 cattacaaaa atttaatgta accaatagaa aaatatggct atttgatacc tatgaaggca
     7621 tgagtgaacc tacagaagat gataaagatt ttaccaattt aagtgctgat aaacaattac
     7681 aaaaatcaaa gaaagatgca gatagtttta tttgggctta tgctacatta gaagacgtaa
     7741 aaaagaatat tgaactaact aaatacccat tagaaaatgt tcattttatt aaaggaaaag
     7801 tagaagacac tattcctgaa aaaaactatt ttaaccactg ttctctttta aggttagata
     7861 cagactggta cgaatctact aaaatagaat tagaatattt ttacccacta ttacaaaaaa
     7921 atggagtaat gattattgat gattatgggc actggcaagg ttgcaaaaaa gcagtggatg
     7981 aatattttgc aacccaaaat ttttgtcctt tactacaaag aattgattac accggcagaa
     8041 taatgaccaa at
//
LOCUS       DAOQPK010000086         7990 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_5025_length_7990_cov_3.956774, whole genome shotgun sequence.
ACCESSION   DAOQPK010000086 DAOQPK010000000
VERSION     DAOQPK010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7990
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5025_length_7990_cov_3.956774"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..1040)
                     /locus_tag="PLU36_05550"
     CDS             complement(195..1040)
                     /locus_tag="PLU36_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HMZ46249.1"
                     /translation="MKKIALFAFALYTVCSVSAQIKMPAPSPSQNISQDFGLGKIELS
                     YSRPSIKGRKIFKEGSELVPLNNVWRTGANGATTLKFTDEVVINGVTIAPGKYGLLSI
                     PGSTNWTLIITKDTTVTSPAAYKQENDIVRIVATTTKMKQKVETFTMQFANIQPESCE
                     LHLMWDKTAISLPITTKIIDRVRTQTENALAAEKVSGNTYYAAANFYYEYDKNYNKAL
                     TCVTKAIEINPKFFWMYLLKAKIEKALGDKVAAKQSAEKCKEIAGQQNNTDYVRMSDE
                     LIKSL"
     gene            complement(1179..2894)
                     /locus_tag="PLU36_05555"
     CDS             complement(1179..2894)
                     /locus_tag="PLU36_05555"
                     /inference="COORDINATES: protein motif:HMM:NF027446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4836 family protein"
                     /protein_id="HMZ46250.1"
                     /translation="MNKIISTLSLLVTIIVFTTSCGKKAPSEAKYIPKDATSIVVINQ
                     KTLEEKLKSGNITLDSFINKMLDTKDSANAASLKKWQDFKNSGIDLDNNLYFFVVQKG
                     SISKGLTTAVNIMAGLKDQAKFEKYIKEQKEFNAKNLIQENNFSYLSSIDTDLSLSWN
                     KEIAIATFYKRDIKGSYDSVGNYIEPDKSETTKDKNEEVKRYFNLKENESVASVAYFN
                     DMFKTKADGYIFGSTNSMLGFLASTPLNIPKVEELLKDNYTASTFNFENGKIEVNNTT
                     YTNPMLSSILKKYSGSSVNTHLAEFFPTQNLNGTILISFKPEMFDGLLKELEIKGMLE
                     AFLAQQGLTSADLFKLLSGEINIAMSDFVIENKEVTQKLYDGSTYTYNKTVPNFKLIA
                     TAPIGDKAAFTKIMDKAVENNLLAKTSTGYTSGEMLASSGMYFMADEKQIVIASSQDF
                     YTTYLNNKTKSNLNSNIIADFKGKSMAAFIDINSIIKGFTPTAKDSTISNKVLAMVNT
                     TFKNFIATTENFKDNSFKGKVTLNMVNEKQNSLVSIINLIDEASKAIKQTTNSTTNSI
                     DENTF"
     gene            complement(2997..5891)
                     /locus_tag="PLU36_05560"
     CDS             complement(2997..5891)
                     /locus_tag="PLU36_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HMZ46251.1"
                     /translation="MNCSKYFSIVLLLSFSLYSNAQKFSEKSANATKWADSIMHTLTN
                     EQKIAQLMIVRAHSNLGEEHINKVTELITQYNIGGLCFFQGGPIRQANLTNYYQSIAK
                     TPLMICIDAEWGLGMRLDSVIKFPRQLMLGAVQDVELIYQVGKAIGLQCKRLGIHVNY
                     APVIDINNNANNPVINDRSFGEDKNKVALFGTKYMQGMQDVGVMACAKHFPGHGDVNV
                     DSHYDLPVINKTKQQLDSLELYPFKQLIKAGVGSIMIAHLFIPSIDSTPNLATSLSKK
                     SVTELLRKELGYNGITFTDGLEMQGVKKFFPDDEVAVQSLVAGNDLLCLPENIQNSIS
                     KIITAINNGRLTWQEIDEKVYRVLIAKYNLGLHKITPIETENITADIDKYTNQLRQEI
                     SKKAITVLKLTNKKILPLFSKRKIAYIGVGINQMNSFAAEVKEIYNAKIFLLDYTDTN
                     NIAQIKKQLKKYKTIIVGLHNFSRRPANNFGINTPTIQFIQYLAEKNNALIFAFGNPY
                     VIKNFCEAKNLIACYEDDNITQLTAANLLQGKFDAKGKLPVTVCNDFKFGDGITYNHY
                     LPFSKKFNSNKFSIIDTIALNAIHQHATPGLSLLVAKKNEIIYYKNFGFTDSTQLHNV
                     TNNTIYDVASITKVAAVTVAIMKLYDEGKIELEKPISNYLPYTLHTNKANITIKDLLL
                     HQSGLVPFISFYKQTIDTVTHKPLQGFFNTSLTEMFTTEVAANMFLVKNFKEKIIKSI
                     LDSELNQKGKYVYSDNNFILLAFIIEAITQKTLNEYVQEIFYKPLQMTTTVFNAQKHF
                     VLNNIAPTELDKYFRQQLIQGYVHDEGAAMLGGVAGNAGLFSNAYDLLQLFQMLLNKG
                     SLNGVQYISEKTINLFTSYQSNESRRGLGFDKPLLNNAEAENPYPCYSISANSFGHTG
                     FTGTCVWVDADKKLIFIFLSNRIHPSRENKLLNTLNVRSNLQETFYNILF"
     gene            complement(5978..6391)
                     /locus_tag="PLU36_05565"
     CDS             complement(5978..6391)
                     /locus_tag="PLU36_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HMZ46252.1"
                     /translation="MEYSKLISVTGLPGLFEMVGSKTDGAIVRNLEDKTTKFVSSRIH
                     NFSHLESIEVYTLGENVNLIDIFKAIESSNEPLPNDKDAKAVKAFIEKVYPEIDVERV
                     YASDMKKMVKWYSVLKANNIELKLSEQAEDAEITE"
     gene            6729..>7990
                     /gene="gpmI"
                     /locus_tag="PLU36_05570"
     CDS             6729..>7990
                     /gene="gpmI"
                     /locus_tag="PLU36_05570"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HMZ46253.1"
                     /translation="MSNKVILIIMDGWGLGKINSSDAIQHANVPFVNSLNSQYPNSTL
                     VTCGEAVGLPDGQMGNSEVGHLNLGAGRIVYQELQRINVAIKTGELAANQVLLNSINY
                     VKKNNKALHFIGLVSNGGVHSHINHLKALIDIANNNGVEKIFIHAFTDGRDCDPKSGI
                     GFIKDLSDYIKNTNTKIASITGRYYAMDRDKRWERVQLAYNALVHGQGELSNNLVTTI
                     QQNYDAGITDEFLKPIINAEVDINEACIKNDDAVICFNFRTDRCREITMALTQQNIEE
                     QKMQKLNLHYTTITEYDATYKNVQVMFDNDNLNNTLGEVLALNGKKQIRIAETEKYPH
                     VTFFFSGGREKEFEGETRMIVPSPKVATYDLQPEMSAIGITDKLIPEIEKETEDFICL
                     NFANPDMVGHTGVFSAVVKAVETVDNCV"
BASE COUNT         2616 a         1187 c         1195 g         2992 t
ORIGIN      
        1 cggtcaatca agttaataat gtccataaaa agagtattta aagtttagtt tagggtgcaa
       61 aagtattgcc accaactctc aaagctacga atttatttat ataatgaata tacttttcct
      121 gatgattttt attggataaa attaaaaaaa ggcgaccaat gtgtcgcctt tttttaattt
      181 tatattacta ctaattataa gctcttaatt aactcatcac tcattcttac ataatctgta
      241 ttgttttgct ggccggcaat ttctttacat ttttctgcac tttgtttggc agctacttta
      301 tctcctaatg ctttttcaat ttttgctttt aataaataca tccaaaaaaa cttaggatta
      361 atttctattg cttttgtaac acaggttaat gctttattgt agtttttgtc gtactcgtaa
      421 taaaaatttg cggcagcata atatgtattg ccacttactt tttctgcagc taatgcattt
      481 tctgtttgtg ttctaactct atctattatt ttagttgtaa tgggtaagct tattgcagtt
      541 ttatcccaca ttaaatgtaa ttcacaactt tcgggttgta tattggcaaa ttgcatggta
      601 aaagtttcta ctttttgctt cattttagtg gtagttgcaa caattcttac aatgtcattt
      661 tcttgcttat atgctgcagg tgacgtaacg gtggtatctt ttgtaataat taatgtccag
      721 tttgtactac ccggaatgga taataaacca tatttaccag gtgctattgt aacaccattt
      781 ataaccactt catctgtaaa ttttaaagtt gtagcaccat ttgcacctgt acgccaaaca
      841 ttatttaatg gcactaattc acttccttct ttaaaaattt ttcttccttt tatagatggt
      901 ctagaatagc ttaattcaat tttacctaaa ccaaaatctt gagaaatatt ttgagatgga
      961 cttggagcag gcattttaat ttgtgcactt acactacaaa cagtgtataa ggcaaaagca
     1021 aataaggcaa tttttttcat attgttttat tttagaaagc aaatgtaggt atttatacat
     1081 tttagcttaa atacataatg atagttggtt gttgaattta gtaaattaaa aagccaccgt
     1141 attatcagtg gctttagtag aagaaaaaaa ttaaaatatt aaaaagtgtt ttcgtctatt
     1201 gagtttgtag ttgaattggt tgtttgttta atagctttac ttgcttcatc tattaaatta
     1261 ataatactta ccaaactgtt ttgtttttca tttaccatat ttaaagttac ttttccttta
     1321 aaactgttgt ctttaaaatt ttctgtggta gctataaaat ttttaaaggt tgtattaacc
     1381 atcgctaaaa ctttattgct aatggttgaa tcttttgcag ttggagtaaa gcctttaatt
     1441 atactgttaa tatcaataaa ggctgccata ctttttcctt taaaatctgc tataatattt
     1501 gaattaagat ttgattttgt tttattgttt agataagtag tataaaaatc ctggctgctg
     1561 gcaattacaa tttgtttttc atctgccata aaatacatac cactagaggc aagcatttcg
     1621 ccagaagtgt agcctgtgct agttttagcc aataaattat tttctacggc tttgtccatt
     1681 attttggtaa atgctgcttt atcgccaatt ggtgctgtag caattagttt aaaatttggt
     1741 actgttttgt tataggtata ggttgagcca tcatatagtt tttgtgttac ttctttattt
     1801 tctataacaa aatcagacat ggcaatgttt atttcgccac ttaataattt aaataaatcg
     1861 gcactggtta aaccttgctg agctaaaaat gcttctaaca ttccttttat ttctaactct
     1921 tttaacaaac catcaaacat ttctggttta aaggaaatta aaatggtacc atttaagttt
     1981 tgtgtaggaa aaaactctgc aagatgggta tttacgcttg aacctgaata ttttttaaga
     2041 atactgctta gcataggatt ggtataggtt gtattgttta cctcaatctt accattttca
     2101 aaattaaaag tagaggcagt ataattatct tttagtaact cctctacctt aggaatattt
     2161 agtggtgtag atgctaaaaa acctaacatg ctattagtgc taccaaaaat atagccatct
     2221 gcttttgttt taaacatatc attaaaataa gcaacagatg caacagattc gttttctttt
     2281 aaattaaaat atctttttac ttcttcattt ttatcttttg tggtttccga tttatccggt
     2341 tctatgtaat ttcctacaga atcataactg cctttaatat ctcttttata aaaagtagca
     2401 attgcaattt ctttattcca cgataaggat aaatcagtat caatagaact taagtaagaa
     2461 aaattatttt cttgaatcaa atttttggca ttgaactctt tttgttcttt aatatatttt
     2521 tcaaattttg cctggtcttt taatcctgcc attatgttta ctgcagttgt tagccctttt
     2581 gaaatggaac ctttttgtac tacaaaaaaa tacagattat tatctaaatc tattccactg
     2641 tttttaaaat cttgccattt ttttaaactt gctgcattgg cagagtcttt agtatcaagc
     2701 attttattaa taaaactgtc taaagttata ttgcctgatt ttaatttttc ttctaatgtt
     2761 ttttgattta ttactacaat agaagttgca tctttaggaa tatactttgc ttcggatgga
     2821 gcttttttac cacaagaagt tgtaaatact attattgtta ctaacaacga tagtgtagaa
     2881 attattttat tcattgataa cttattttgg atacgaatat aaaaaatgta caataaacaa
     2941 tacagtaaag aagtataaat tttaaaaatt aatgtttaaa aggaagaatc cttatttcaa
     3001 aataaaatat tgtaaaaagt ttcttgcaaa ttgcttctaa catttagtgt atttaaaagt
     3061 ttattttctc tgcttgggtg tattctgtta gaaaggaaga tgaatattaa tttcttgtca
     3121 gcatctaccc atacacatgt acctgtaaaa cctgtgtggc caaaagagtt tgccgaaata
     3181 gaataacaag gataggggtt ttcggcttct gcattattca acaatggttt atcaaaccct
     3241 aatcctcttc tgctttcatt gctttgatag gatgtaaata aattaattgt tttttctgaa
     3301 atatactgca caccatttaa gctgccttta tttaaaagca tttgaaatag ttgtagcaaa
     3361 tcgtaagcat tgctaaataa gccagcattc cctgcaacac cacctaacat tgctgctcct
     3421 tcatcgtgta cataaccttg tattagttgc tgtctaaagt atttatctag ttcagtaggt
     3481 gcaatattat ttaatacaaa atgtttttgt gcattaaata ctgtggtagt catttgtaat
     3541 ggcttataaa atatttcttg tacatattca ttcaaagttt tttgtgttat ggcttcaata
     3601 ataaatgcta atagaataaa attgttatcg ctataaacat atttcccttt ttggtttaat
     3661 tcactgtcta aaatgctttt aataatcttc tctttaaaat tttttaccaa aaacatattt
     3721 gctgctactt cggtagtaaa catttcggtt aaggaagtat taaaaaaacc ttgtaagggc
     3781 ttgtgggtaa cggtatctat ggtttgtttg taaaaagaaa taaaaggcac taacccagat
     3841 tggtgcaaca acaaatcttt aattgttata ttggctttat tggtgtgtaa agtgtaaggt
     3901 aaataattgc taattggttt ttctaactct atttttcctt catcatataa tttcataatg
     3961 gcaacagtta ctgctgctac tttggtaatg gaggctacat cgtaaatagt attgttggta
     4021 acattgtgta gttgagtgga atcagtaaaa ccaaaatttt tataataaat tatttcattt
     4081 tttttagcaa ctaataaaga taagcctggt gttgcatgct gatggatagc atttaaagca
     4141 atggtatcaa taatagaaaa cttatttgag ttaaattttt tagaaaatgg aagatagtgg
     4201 ttataggtaa ttccatctcc aaacttaaaa tcattgcaaa ctgttacagg caatttacct
     4261 tttgcatcaa acttgccttg cagtaagtta gctgctgtta actgtgttat attgtcatct
     4321 tcgtaacaag caattaagtt tttagcctca caaaaatttt taattacata agggttacca
     4381 aaagcaaaaa ttaaagcatt atttttttca gccaggtatt gaataaattg aatggttggt
     4441 gtattaatgc caaaattgtt tgctggcctt ctgctaaaat tatgtaagcc aacaattata
     4501 gttttatact tttttaattg tttttttatt tgtgcaatat tgttcgtatc ggtataatca
     4561 agtaaaaata ttttagcatt gtatatctct tttacttcgg ctgcaaagct gttcatttgg
     4621 ttaatgccaa ctcctatata agcaattttt cttttgctaa aaaggggtaa tattttttta
     4681 ttggttagtt ttaaaacggt gattgctttt ttactaattt cttgtcttag ttggttggta
     4741 tatttgtcaa tatcagcagt aatattttct gtttcaatag gtgttatttt atgtaagcct
     4801 aaattatatt ttgcaattaa aaccctgtaa actttttcat ctatttcttg ccaggtcaat
     4861 cttccgttat taattgcagt gataattttt gatatgctat tttgaatatt ttctggcaag
     4921 caaagcaaat cattaccagc aactaagctt tgtacagcta cttcatcatc tggaaaaaac
     4981 ttttttacac cttgcatttc taaaccatca gtaaaagtaa ttccattata tcctaactct
     5041 tttcttaaaa gttctgtaac acttttttta gaaagagagg ttgccaaatt tggagtactg
     5101 tctatagatg ggataaataa atgggctatc attatactgc caacaccagc ttttattaat
     5161 tgtttaaaag ggtataactc taggctatct aattgttgtt tagttttatt aattacaggt
     5221 aaatcgtaat ggctgtcaac atttacatca ccatgtcctg gaaaatgttt tgcacaagcc
     5281 attacaccaa catcttgcat tccttgcata tattttgtac caaacagggc aaccttattt
     5341 ttatcttctc caaagcttct atcattaatt acaggattgt tggcattatt gttaatgtca
     5401 attacaggag cataatttac atgaatacct aaacgtttgc attgtaagcc aattgctttt
     5461 cctacctggt atattaattc aacatcttgt acagcaccaa gcataagctg acgaggaaat
     5521 tttataacac tatccagcct catgcctaag ccccattctg catctataca aatcattaag
     5581 ggtgttttgg caatagattg gtagtagttg gtaaggttag cttgcctaat agggccacct
     5641 tgaaaaaaac ataatccacc tatattgtat tgtgttatta actcagtaac tttattaatg
     5701 tgctcctctc ctaagttgct atgagctcta actatcatta actgtgcaat tttttgctca
     5761 ttggttaatg tatgcattat actatctgcc catttggtag catttgctga tttctcagaa
     5821 aatttttgag cattactata caacgaaaag cttaagagta atacaatact aaaatatttg
     5881 ctacaattca ttaatttaat ttaaaaatat ttgctaatgt acaaacaaaa aagccacaga
     5941 tgaatgtggc tattaaaagt taatttgtat ttaaaaatta ttcggttatt tctgcatctt
     6001 cggcttgttc acttaattta agttctatat tattagcttt taaaacacta taccatttta
     6061 ccattttttt catatcgctg gcatacactc tttctacatc tatttcagga taaacttttt
     6121 cgataaatgc tttaactgct tttgcatctt tatcgttagg taatggttca ttactgcttt
     6181 caattgcttt aaaaatatca atcaaattta cattttcacc aagtgtataa acttctatac
     6241 tttctaaatg agaaaaatta tgaatacggc tacttacaaa tttggttgtt ttgtcttcta
     6301 aatttcttac aatagctcca tctgttttac tacctaccat ttcaaataaa cctggcaaac
     6361 ccgtaactga aattaattta ctatattcca ttatttaatt ttaatggctg caaaataaag
     6421 gtatttttat attacagaat agtataaaag ttataagttt tgtaaataaa aaaagctgat
     6481 gattttaacc atcagctacc ccttagaacc ccctgaataa gaattgaagt acttcttcaa
     6541 atgcaatgaa acaacattta tttaaataca gcaataccct taattgggta tttttaaaaa
     6601 ttcatttcac tatttagttg tatagataac gaaagcaatt atattaatga acctacattt
     6661 gtagaatatt ttttgttaca cttaaactaa aaatataatt aagcattaaa agcaaatatc
     6721 tttacgtaat gagcaataaa gtaattttaa ttatcatgga tggatggggt ttaggtaaaa
     6781 taaattcgtc cgatgccatt caacatgcca atgtaccttt tgttaattct ttgaatagcc
     6841 aataccctaa tagtacttta gtaacctgtg gcgaagctgt tggtttgcct gatggacaaa
     6901 tgggtaacag cgaagttggg cacttgaatt taggtgctgg cagaattgta tatcaagaac
     6961 tccaaagaat taatgtagcc ataaaaacag gcgaacttgc tgccaaccag gttttattaa
     7021 attctattaa ttatgtaaaa aaaaataata aagctttaca ttttattggc ttggttagta
     7081 atggtggtgt acactctcat atcaatcatt taaaagctct aattgatatt gcaaataaca
     7141 atggggttga aaaaatattt atacatgcat ttaccgatgg cagagactgt gaccctaaaa
     7201 gtggaatagg atttattaaa gatttaagtg attatattaa gaataccaat acaaaaatag
     7261 cttcaataac aggtaggtat tatgcaatgg atagagataa aagatgggag agagtgcaac
     7321 tagcttacaa tgcattagtt catggtcaag gcgaactaag caataattta gtaaccacta
     7381 ttcaacaaaa ttatgatgca ggtattacag atgagttttt aaaacctatt attaatgcag
     7441 aagtggatat taatgaagcc tgtataaaaa atgatgatgc tgttatatgc tttaatttta
     7501 gaacagaccg ttgcagagaa attacaatgg cacttaccca acaaaatata gaagaacaaa
     7561 aaatgcaaaa attgaatttg cattacacaa ccattacaga atacgatgct acttataaaa
     7621 atgtgcaagt aatgtttgat aatgataatt taaacaatac acttggcgaa gtattagctc
     7681 tgaatggaaa aaaacaaatt cgtattgctg aaactgaaaa atatccgcac gtaacttttt
     7741 tctttagtgg aggcagagaa aaagaatttg aaggagaaac cagaatgatt gttccatcgc
     7801 ctaaggtggc aacttatgat ttacaaccag aaatgagtgc aataggaatt acggataaac
     7861 taatacctga aattgaaaaa gaaaccgaag attttatatg cttgaatttt gcaaacccag
     7921 atatggtagg gcatacaggc gtttttagtg ctgtggtaaa agctgtagaa acagtagata
     7981 attgtgtgaa
//
LOCUS       DAOQPK010000087         7901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_5092_length_7901_cov_4.409763, whole genome shotgun sequence.
ACCESSION   DAOQPK010000087 DAOQPK010000000
VERSION     DAOQPK010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7901
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5092_length_7901_cov_4.409763"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PLU36_05575"
     CDS             complement(<1..686)
                     /locus_tag="PLU36_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HMZ46254.1"
                     /translation="MKLSQFRYDLPLNLIAQHPTKKREDSRLLVVNRKTGNMENKHFR
                     DVLDYFDDKDAFVVNNTKVFSARMYGRKEKTGAKIEVFLLRELSSQNRLWDVIVDPAR
                     KIRVGNKLYFGESEELVAEVIDNTTSRGRTIKFLWEDTEESFRKMLETLGETPLPKYI
                     KRKPDEEDKERYQTVYAKHEGAVAAPTAGLHFSKELIKRCEIKGIRFAEITLHTGLGT
                     FRPIEVEDLS"
     gene            complement(755..967)
                     /locus_tag="PLU36_05580"
     CDS             complement(755..967)
                     /locus_tag="PLU36_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HMZ46255.1"
                     /translation="MDQTILEDLVSKTIPFGKYKGKIYCELPEVYIEWFARKGFPKGR
                     VGTLLQTMYEINLNGLQHLLNPLKKK"
     gene            complement(992..3817)
                     /locus_tag="PLU36_05585"
     CDS             complement(992..3817)
                     /locus_tag="PLU36_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HMZ46256.1"
                     /translation="MKNKLGKIFKYSGISIGSILVLMFLLPYLFPGFVSDKIKKWANK
                     SIESELNFSKARLSFFNHFPSLTLTLYDVNLKGSAPFKNDTLITANEIALGVDILSVF
                     KNTLKIDEVYVTNGKIKILVSKNGIPNYNIYKSSSTTKEHSEVHEESDAALKLDRIQI
                     ENCALEYNDQSLPMLLKAGKVNYLGQGDLSKEIFDLTSKIEMQDFDFIYNTQPYLSNK
                     NLKAQLITRINTHSLELDFQKNNLNINHLPIEVTGKLNFLSNGYSLQFNANTPSTKLK
                     NLFTALPPAFVQWVDKTNVDGKLALNVALNGNYIKSKNQAPDLKFTIQVNNGSFSIAN
                     SNEPIKNIYLNASIDIPKLNTQNLFLKVDTLNATLGKNYLKGAFEAKNFAEPFIKGHL
                     NVNMDLEKWNNILNIDSIAGITLKGNFKTEANFNGVYNTQKNTFPITNATIQWQNGYL
                     KTKYYASAINNINIDATIQNKSGTTKDVLINVKPISFLFEEQPITVSANLLNLNNLNY
                     SISSKGNLQIGKLYKLFAIDGYNINGNIKTDFTLQGSQQDALAGNYHKLNNKGTIELK
                     DINLTTPYLPNKFTITNGNFYFNNDKLIANNVTINYLNNTAVFKGKFDNIINYLLQPN
                     AALKADLFLNSNKLNVQDFMAFAKDSTTLINTNKTEKNSTPTSNSTGVILLPTNLNIN
                     FNAAIKTVEYNHLQLSNVLTNASIQNGNLKIHNADFNVIGSPIKMTAKYSPINFKKAH
                     FTYNISATELDIQKAYKEIQFFRDMLTSAKKMKGLVSINYSINGLLNDSLYPILPTLK
                     GNGTISIKNAKFYGFKLFNAISKKTNKDAIDNPDLSKHNIILKSKIANNIITIEKIKL
                     RVAGFRPKFEGQVSLDGRLNLKARLGLPPFGIFGIPLSITGTQENPIVKFKRGKKSTD
                     LEETEADEEDKKDAAEAEQKEQQQKNK"
     gene            complement(3894..4607)
                     /gene="pyrH"
                     /locus_tag="PLU36_05590"
     CDS             complement(3894..4607)
                     /gene="pyrH"
                     /locus_tag="PLU36_05590"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HMZ46257.1"
                     /translation="MLPKYKRVLLKLSGEALLGQNNGDPFDPKIIEQYAYDIKEVTNL
                     GVQVAIVIGGGNIYRGMNEADSGIERAHGDYMGMLATVINAMAMQAMLEKIGVYTRLQ
                     SAINMEQVAEPYIRRKALRHLEKGRVVIFGAGTGNPYFTTDTAGSLRAIEMKADVILK
                     GTRVDGIFTADPEKDATATKYDKLSFKECITNNLKVMDMTAFTLCMENKLPIIVFNMN
                     KPGNLLNVVEGLNVGTLVN"
     gene            4872..5861
                     /locus_tag="PLU36_05595"
     CDS             4872..5861
                     /locus_tag="PLU36_05595"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HMZ46258.1"
                     /translation="MKSNFYTLIMRLTFCSIIILQGTKSTAQIYYSNFVRSDRQWEFD
                     VNLGATNFLGDVGGGKGKGTFFLKDLNLNSTNLFYGANLTYYPNQWLGIRGNFNIGKL
                     SGFDSLIKNQGNVEQNRKKRNLGFRTNIAEVNVAFEIYPTAYFVRNESFFNSKLRPYF
                     TVGIGAFKFNPQGIYEAPNGTKKWVDLRPLKLEGQGMAEYGDRKEYSNIALQIPFGFG
                     IKYFINEKLYLAAEIMQRYTFTDYVDDVSKTYIDPALFAKYLPKDKVAIAEQMMFKRA
                     LVNNRPVSEFVGKERGDSKNKDYYLSLFVKFGFRFGARDTDMSCPTGRRRSYY"
     gene            complement(5932..7020)
                     /gene="pdxA"
                     /locus_tag="PLU36_05600"
     CDS             complement(5932..7020)
                     /gene="pdxA"
                     /locus_tag="PLU36_05600"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HMZ46259.1"
                     /translation="MSNELYKPIIGFTCGDLNGIGIELIIRSLHDSRILEICTPIVFA
                     SNKSINFYRKSLADINFSYSSCKDFQRINTKQVNVFNCWEEEVQITPGELNSIGGKYA
                     LISLNKATQTLKEGNIDALVTAPIHKKNIYGDNFKYNGHTPYFKDVFEAKDVVMLMVA
                     ANMKVGLVTEHVAIADVAKHITKDSIVSKLKIINKTLQTDFGIDKPRIAVLGLNPHTG
                     DEGLIGTEEETIIKPAIKEAKQEMIVFGPYSADAFFTRGQHEKFDAVLAMYHDQGLIP
                     FKSLALGEGVNYTAGLNVIRTSPDHGTAFDIAGKAKANYASFLEAIYTAIDIARYRAT
                     YKELRKNQLNKMSAKLIVNGVDEKIEEQ"
     gene            complement(7062..>7901)
                     /gene="rlmN"
                     /locus_tag="PLU36_05605"
     CDS             complement(7062..>7901)
                     /gene="rlmN"
                     /locus_tag="PLU36_05605"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HMZ46260.1"
                     /translation="KTDTIQHSDDGTVKSRFTTFDGYKVEGVLIPTEERKTACVSSQI
                     GCSLTCKFCATGFMDRKRNLTYDEIYDQVVLLNQQSEKIFNQKLTNIVFMGMGEPLLN
                     YNNVLQAIERISAEDGLFMSTKRITVSTAGVAKMIKKLGDDKVRFKLALSLHAANDKK
                     RNEIMPINENNNIRSLTEALNYFYKQTGNEITFEYILFKDFNDSQQDADELIKLYRQI
                     PVDLVNIIEYNPIENTTFTKPDDATTTNFMEYLESKKVNARLRKSRGKDIDAACGQLA
                     NKG"
BASE COUNT         2456 a         1203 c         1151 g         3091 t
ORIGIN      
        1 ttactaaggt cttccacttc aattggtcta aaagtaccta agcctgtatg taaagtaatt
       61 tctgcaaacc taataccttt tatttcacaa cgtttaatta actctttact aaagtgcaag
      121 ccagcagttg gtgctgcaac agcaccttcg tgttttgcat aaacagtttg atacctttct
      181 ttgtcctctt catctggctt acgtttaatg tattttggta atggagtttc gcccaaggtt
      241 tctaacattt ttctaaaact ttcttctgta tcttcccata aaaattttat tgttcttcca
      301 cggctggttg tattgtcaat tacttctgca acaagttctt cactttcgcc aaaatatagt
      361 ttattgccta cacgtatttt acgagcaggg tcaacaatta catcccataa acggttttgg
      421 ctacttaatt ctctaagtaa aaaaacttca atttttgcac cggttttttc ttttctgcca
      481 tacatacgag cagagaaaac tttagtgttg tttactacaa acgcatcttt atcgtcaaaa
      541 taatctaaaa catcacggaa gtgtttattt tccatgttac ctgttttacg atttacgact
      601 aataaacggc tgtcttcacg ttttttggtt gggtgttgtg caataaggtt taaaggtaga
      661 tcgtacctga attgagataa tttcatgtta cggcatgatt tagttaaaaa atgaaaggct
      721 gcaaaagtaa tagatttata ggtataaaac ttttttactt ctttttcaac ggatttaata
      781 gatgttgtaa gccgtttagg tttatttcgt acatggtttg tagcaatgtt ccaactcttc
      841 ctttaggaaa gccttttctt gcaaaccatt caatataaac ttctggcaat tcacaataaa
      901 tttttccttt gtatttgccg aatggtatgg ttttacttac tagatcttct aaaatagttt
      961 gatccataga tattaagagg gctaaattgt attatttatt tttttgttgt tgttcttttt
     1021 gttctgcttc tgcagcatct tttttatctt cttcatccgc ttcggtttct tctaaatcgg
     1081 ttgatttttt tcctcgttta aatttcacaa ttggattttc ttgtgtgcca gtaatagaaa
     1141 gtggaatgcc aaatatgcca aatggtggca agcctaatct ggcttttaaa tttaacctac
     1201 catctaaact aacctgccct tcaaactttg gcctaaagcc ggcaactcgt agttttattt
     1261 tttcaatggt aataatatta ttagcaattt ttgattttag tattatgtta tgtttagata
     1321 aatccgggtt atctattgca tctttatttg tttttttact aattgcatta aacaacttaa
     1381 aaccataaaa ttttgcattt ttaatactta ttgttccatt accttttaag gttggtaaaa
     1441 tggggtataa gctatcattc aataacccat ttatagaata attaatgcta accagacctt
     1501 tcattttttt tgcagaagtt aacatgtctc taaagaattg aatttcttta tatgcttttt
     1561 gaatgtctaa ttctgtagca gaaatgttgt aagtaaaatg tgcttttttg aagttaatgg
     1621 gggaatattt tgcagtcatt tttattggtg agccaataac attaaaatct gcattatgta
     1681 tttttaaatt tccattttgt atgcttgcat tggttaaaac attactaagt tgtaagtggt
     1741 tatattcaac agttttaatg gcagcattaa agttaatgtt taagtttgta ggaagtaaaa
     1801 taaccccagt agagtttgag gttggtgtgc tatttttttc tgttttatta gtgttgatta
     1861 aggttgtaga atcttttgca aaagccataa aatcctgcac atttaacttg ttactattta
     1921 aaaacaaatc tgcttttaat gctgcattgg gttgtaataa gtaattaata atattatcga
     1981 acttgccttt aaaaacagcc gtattgttta aataatttat agttacatta ttggctatta
     2041 acttatcatt attaaaataa aaattaccat ttgttatagt aaacttatta ggtaaataag
     2101 gtgttgttaa attaatatct ttaagttcta tagtgccctt attgtttagt ttatgatagt
     2161 ttcctgcaag tgcatcttgt tgtgagcctt gtaaggtaaa gtctgttttt atatttccgt
     2221 ttatattgta accatctatt gcaaacaatt tatacaactt acctatttgc aaatttcctt
     2281 ttgatgaaat ggaatagtta agattattta aattaagtaa gttggcactt actgttattg
     2341 gctgctcttc aaataaaaaa gaaatgggtt taacatttat tagtacatct ttggtagttc
     2401 ctgatttatt ttgaatggtt gcatctatat taatattatt aattgcactg gcatagtatt
     2461 ttgtttttaa atatccattt tgccattgta tagtagcatt tgttattgga aatgtatttt
     2521 tttgggtatt ataaactccg ttaaaatttg cttctgtttt aaaatttcct tttaatgtta
     2581 tgcctgcaat gctatctatg tttaaaatat tattccattt ttctaaatcc atattaacat
     2641 ttaaatgccc cttaataaat ggttcagcaa aattttttgc ttcaaaagca cctttcaaat
     2701 agttttttcc aagtgtagca tttaaagtat ctacttttaa aaataaattt tgggtgttta
     2761 gttttggtat atcaatggag gcatttaaat aaatattttt gataggttcg ttgctgtttg
     2821 caatactaaa tgaaccattg tttacttgga tagtaaattt taaatcgggt gcctggtttt
     2881 tacttttaat atagtttccg tttaatgcta catttaaggc aagttttcca tctacattag
     2941 ttttatcaac ccattgaaca aaagcaggag gaagtgcggt aaacaaattt tttaattttg
     3001 tagaaggtgt attggcatta aattgcaagc tatatccatt acttaaaaaa tttaattttc
     3061 ctgtaacttc aattggtaaa tgatttatgt ttaaattatt tttttgaaaa tctaactcta
     3121 aagaatgtgt attaatacgt gttattagct gtgctttaag atttttgtta cttaagtaag
     3181 gttgtgtatt ataaataaaa tcaaaatctt gcatttctat tttagaggta agatcaaaaa
     3241 tttctttact taaatctcct tgtcctaaat aatttacttt accagctttt aacagcattg
     3301 gtaaagattg atcgttatat tctaaagcac agttttctat ttgtattcta tctagtttta
     3361 gtgcagcatc gctttcttca tgtacctcag aatgttcttt ggttgttgaa ctgcttttat
     3421 aaatattata attaggaatt ccgtttttac taactaaaat tttaatttta ccattggtta
     3481 cataaacctc atctattttt aaagtgtttt taaatacact taaaatatct acacccaatg
     3541 caatttcatt agctgtaatt aatgtatcat ttttaaatgg tgcagaacct tttaaattaa
     3601 catcatataa agtaagagtt aaagaaggaa agtgattgaa gaaggataat cttgctttag
     3661 agaaatttaa ttcagattca atagatttat tagcccattt ttttatttta tcagatacaa
     3721 accctggaaa taagtaaggt aataaaaaca ttaataccaa aatgctacca atacttattc
     3781 ctgaatattt gaatatttta cctaatttat ttttcattat aagcctgtat aaaatagaaa
     3841 actgttagca aaagtactaa cagtttttaa agaatatttt tactattatt taattaattt
     3901 accaatgtac ctacatttaa accttctact acatttagca aattaccagg tttgttcata
     3961 ttaaaaacaa taattggcaa tttattttcc atacacaaag taaaagcagt catatccatt
     4021 acttttaaat tgtttgtaat acattcttta aaactaagtt tatcatattt agtagctgta
     4081 gcatcttttt cagggtcggc agtaaaaata ccatctactc ttgtgccttt taaaatcaca
     4141 tctgctttca tttcaatggc tcttaagcta ccagcagtat ctgttgtaaa ataagggtta
     4201 cctgttcctg ctccaaaaat tacaacacgc cctttttcta aatgtcttaa agcttttctt
     4261 ctaatatatg gctctgccac ttgttccata ttaatagcac tttgcaaacg ggtgtacaca
     4321 ccaatttttt ctaacatagc ctgcatagcc atagcattaa ttacagtagc caacataccc
     4381 atataatctc catgtgcacg ttcaatacca ctatcagctt cattcatacc tctatatatg
     4441 tttccacctc caataacaat tgcaacctga actcctaaat tggttacttc ttttatatca
     4501 taagcatatt gttcaattat tttaggatca aatggatctc cattattttg tcctaataaa
     4561 gcttctccag ataattttaa taatactctt ttgtatttag gcaacatgtt ataatattta
     4621 tgtgtgcaaa taaccactat ttttattatg cagccaatag tattgtaata aatttaatta
     4681 tataggctat ttaagttaac tatttgccct ttgttttgtt agtttttagc ttaaaatagc
     4741 taaaagatag agcagaaata ttttttttac tgatttatat aataaatttt actagctatc
     4801 gttttaatat aacagcccca aagcaattag tttaaaggct gaaatattta atatccaata
     4861 tcaaacattc tatgaaatcc aatttctaca cgctaataat gcgtttaacc ttttgcagta
     4921 taattatatt gcaaggtaca aaatcaacag cacaaattta ttattccaat tttgttcgta
     4981 gcgaccgcca gtgggagttt gatgtaaacc ttggtgcaac taatttttta ggagatgttg
     5041 gtggtggcaa gggtaaagga accttttttt taaaagacct taatttaaat tctaccaatt
     5101 tgttttatgg tgccaacctt acttattacc ctaatcaatg gttgggtatt agaggtaatt
     5161 ttaatattgg caaactttct ggttttgata gtttaataaa aaaccagggt aatgtagaac
     5221 aaaatagaaa gaaaagaaac cttggattta gaactaatat tgcagaagta aatgtagctt
     5281 ttgaaattta cccaactgct tattttgtta gaaatgaaag tttttttaat agcaagttga
     5341 ggccttattt tactgtaggt attggtgctt ttaaatttaa tccacaaggt atttacgagg
     5401 ctcctaatgg tacaaaaaaa tgggttgatt taaggccatt aaaattagaa ggacaaggta
     5461 tggcagaata tggagataga aaagagtatt ctaacattgc attacaaata ccttttggct
     5521 ttggaattaa atattttata aatgaaaaac tttatttagc agcagaaata atgcaacgat
     5581 atacatttac cgattatgta gatgatgtaa gtaaaaccta tattgaccca gccttatttg
     5641 caaaatattt acctaaagat aaagttgcca ttgctgaaca aatgatgttt aaaagggcat
     5701 tagtaaacaa taggccagta agtgaatttg ttggaaaaga aagaggcgat tcaaaaaata
     5761 aagattacta tttatctctt tttgtgaaat ttggatttag gtttggtgca agagatactg
     5821 atatgagttg ccctacaggt agaagaagaa gttactatta ataatcttgt tgtttgttat
     5881 attgaaaatt gacaggtatg ctaaacacta tccaatttgg atagtgtttt tttattgctc
     5941 ttctattttt tcatcaacac catttacaat tagtttagca ctcattttat tcagttgatt
     6001 ttttcttaat tctttataag tggctctata tcttgcaata tcaattgctg tataaatggc
     6061 ttctaaaaaa gaggcataat ttgcttttgc ttttcctgca atatcaaaag cagtaccatg
     6121 gtcggggctt gtgcgtatta catttaagcc tgctgtataa ttaacacctt cgccaagggc
     6181 taaagattta aaaggtatta aaccttggtc gtgatacatt gctaaaacag catcaaattt
     6241 ttcatgttgg cctcttgtaa aaaatgcatc tgcactatat ggcccaaaca ctatcatttc
     6301 ttgtttggct tcttttattg caggcttaat aatggtttct tcttctgttc caattaatcc
     6361 ttcatctcca gtatgtgggt ttaagcctaa aacggcaata cgtggtttat ctattccaaa
     6421 atctgtttgt aaagttttat ttattatttt aagtttacta acaatactat ctttagtaat
     6481 gtgtttagca acatcggcaa tggcaacatg ttctgttacc aagcctactt tcatattagc
     6541 tgcaaccatt aacattacca catcttttgc ttcaaaaaca tctttaaaat acggggtatg
     6601 accgttgtat ttaaagttat cgccataaat atttttttta tggatagggg ccgtaactaa
     6661 ggcatcaata tttccttctt ttaaggtttg tgtggctttg tttaatgaaa ttaaagcata
     6721 tttaccgcca atgctgttta actcaccagg tgttatttgc acttcttctt cccagcaatt
     6781 aaaaacattt acctgtttgg tatttatgcg ttgaaagtct ttacagcttg agtatgaaaa
     6841 attaatatct gctaaacttt ttctgtaaaa gttaatactt ttattactag caaaaactat
     6901 gggtgtacat atttctaaaa tacggctatc gtgtaatgaa cgaataatta attcaatacc
     6961 aattccattt aaatcgccac aagtaaatcc tattattggc ttatataatt cgttgctcat
     7021 tataagtgta ttaattattg taaaattaat tataaaatgt tttatccttt attagcaagt
     7081 tgcccacagg cagcatctat atcttttcct ctgctttttc ttaaacgggc atttactttt
     7141 ttactttcta aatactccat aaaattggtt gtggttgcat catctggctt ggtgaatgta
     7201 gtattttcta ttgggttata ttcaataata tttactaaat ctaccggtat ttgcctatat
     7261 agttttataa gttcatcagc atcttgctgc gagtcgttaa aatctttaaa taaaatgtat
     7321 tcaaaggtta tttcattacc agtttgtttg taaaaataat ttaatgcttc tgttaaagat
     7381 ctaatgttgt tattttcatt aatgggcatt atctcatttc gttttttgtc gtttgcagca
     7441 tgtaggctta aggctaattt aaaacgtact ttatcatcgc ctaacttttt aatcattttt
     7501 gcaacacctg ctgtagatac agttattctt ttagtgctca taaacaaacc atcttcggca
     7561 cttattcttt caattgcttg tagaacattg ttgtagttaa gcaaaggctc acccataccc
     7621 ataaaaacaa tattggtaag cttttggtta aatatttttt cgctttgctg atttagtaaa
     7681 actacttggt cgtaaatttc atcgtatgta agatttcttt ttctatccat aaaacctgtg
     7741 gcacaaaatt tacaggttaa actgcaccca atttgagaag aaacacaagc tgtttttctt
     7801 tcttcggttg gtattaaaac gccttctact ttatatccat caaaagtagt aaacctgctt
     7861 tttacagtcc catcatcact atgctgaatg gtatctgttt t
//
LOCUS       DAOQPK010000088         7823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_5149_length_7823_cov_4.695675, whole genome shotgun sequence.
ACCESSION   DAOQPK010000088 DAOQPK010000000
VERSION     DAOQPK010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7823
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5149_length_7823_cov_4.695675"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PLU36_05610"
     CDS             <1..261
                     /locus_tag="PLU36_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HMZ46261.1"
                     /translation="GGYKAFRNYALKYFTRERKIVLLGGKTGSGKTDVLKKLNELYNK
                     WIDNYKEGPLLVIDCDKNKFAEHDEHLGEVISKVDSMLFGLF"
     gene            268..753
                     /locus_tag="PLU36_05615"
     CDS             268..753
                     /locus_tag="PLU36_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46262.1"
                     /translation="MKKLGLTFLFFSIFLLAYGQDKLDRNTIIGKWVAFGMLYNEVYY
                     NLDTDSFYISPEKYDLLLSQNEDTIGAKKYLRNLMLSILPFNFNFKKNGNCTFENKNI
                     FERTTYAFDELKKIITIFNATTGKFINLEAYMKDNDLYINMNKDDSNSIKGCIIFKKI
                     K"
     gene            833..1927
                     /locus_tag="PLU36_05620"
     CDS             833..1927
                     /locus_tag="PLU36_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HMZ46263.1"
                     /translation="MLTLIRWMVGLLFIFSGLIKANDPLGLSYKMQEFFEAWQLHALH
                     NYTLFFAYGMNVLEVVAGIAVIVGWQIKKTVWLLLTLIVFFTFLTAYVLFSGKIKACG
                     CFGDCVPLTPVQTFTKDIILLILILILLFKVNSFRSSLNNNLSFAIVFIALIGTILFQ
                     NYVLKYLPVLDCLPYAKGKNLVTGMQLPKGALPDSFAITFKYKKDGKLIEFDQNNFPA
                     DFDDTYEYIDRYDKLVRKGTALPAITDFSLTTVFGNDTTQALLNSKTKYILLFAKDFN
                     NINKWDNEDFKKGVEIAKNKNIPFYIVTANVSFAKEHFNNLTILSCDAVAIKTAARVN
                     PTYFLMNGADVDNKISYANNKELLKALTTF"
     gene            1953..2990
                     /locus_tag="PLU36_05625"
     CDS             1953..2990
                     /locus_tag="PLU36_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ46264.1"
                     /translation="MFSFIIKKLGYGILVMFGVVVIVFFLFQGFGDPSRLIIGQTGDS
                     TTIKNIRKELALDQPKWKQFTMYLNDISPLGFYSKTERKEKNIKGYFVGNVGIKLPYL
                     RRSYQTKKNVSTILSEAIPQTIVLAFAAMFIAIVFGIALGVVAALKQNTWLDTGSIFT
                     SVLGISAPSFFMGIVIAYLFGFVLNNYTNLPLTGSLYEIDAFNGKQLQLKNLILPAIT
                     LGIRPMAIITQITRSAMLDVLNQDYIRTAYAKGLSTKMVVWKHALKNALNPVITSITG
                     WFAELLAGAFFVEYIFGWNGIGKLTVMALENLDFPVVMGSVLLTASFFILVNIIADLL
                     YSYIDPRIKLS"
     gene            complement(3001..4146)
                     /locus_tag="PLU36_05630"
     CDS             complement(3001..4146)
                     /locus_tag="PLU36_05630"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HMZ46265.1"
                     /translation="MSLNNNSTIKKVGILGGGQLGRMLLQAAANYVVETFVLENDENC
                     PSAHLCTHFIKGDIKDFETVYNFGKKVDALTIEIESVNVEALEQLEKEGVKIYPSPSV
                     IKTINNKILQKQFYQQNQIPTAEFVVTNQANEIINHVNLLPAVHKIAQGGYDGKGVQT
                     INTKEDAVKGFNAPSVLEKCITITKEIAIIIAVNDKKETAVYPLVEMIFDPYLNLLDY
                     QFSPAKLPENIVYKVEAVALSVVKNLNSPGIFAVELFVDNNNNIWVNETAPRVHNSGH
                     HTIEGNYSSQYDMLWRIILNFPLGNTSAILPSAIVNLIGSNNYNGNAIYDGLENILSM
                     DNVFVHLYGKIITKPGRKMGHVTILSSDYSDLTYKANKIKNSLKVVA"
     gene            4518..>7823
                     /locus_tag="PLU36_05635"
     CDS             4518..>7823
                     /locus_tag="PLU36_05635"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMZ46266.1"
                     /translation="MEVGFYANHLTGCSPELIQFKDTSKGNPTYWKWDLGNGTISYQK
                     NPSATYFNPGSYTIKLFISNQNGEDSITKTSYITIYEKPTVNFTANKNTGCAPLQIQF
                     NDLSVAGNDSITQWLWDFNDGNISSLKNPNHIYTQQGEFDVKLKVTTMNGCFSAIEKK
                     DFIKTEGLLQAGFNYTTLGANCTAPTTVQFTNTSTGSGNLTFNWSFGDGATSTIINPT
                     HTYSTTGSFTVQLVVTNALGCTDTLKIDHAITIGSVQANFSLPDSICAGTKLDLMNAS
                     SPTVVSNNWYFGNGLNSTQINPSVTFTNAGTYTIKLVSNFGTCLDSVEKSIKILSKPE
                     ANFSAINTSGCSLPLQTQFTNSSMGGESYQWNFGNGITSTLHSPTHTFTNFGIYNVML
                     QVTAENGCTDTLLMDSVVKIIPIKINSIAIDPEEGCVPLLVSFNANITAVDSIATYTW
                     YFGDNTISNEKEPKHTYNQAGTYDVKLVITSTNGCVDSLIVFNAVKVGEKPRADFEAT
                     PREGCTITGINFTDLSTNATVDKWYWSFGDGKRSYNQNPLTTYQDTGWFNVRLIVWSN
                     GCSDTMVKANYIHIKPPKANFSFKAEDCNNKLTVQFFDSSKGAVTYKWFFGDGDTSNI
                     ANPIHTYSKSGTYQVLLVVVNGDCSHSRIRTVVVDDNNGKLVISDTVVCRNTKVNFSL
                     TNLNTISAATYTWSVGEGNLITTGGGPLTEYVYTNAGKFNVWVAIDYANGCKDTIEYN
                     SIKVYGATAKFSSPENTFCSGSTIVFNDSSFTDGTHAITNWLWNYGDGTVENNNNAAA
                     SHFYATGGSFDVKLKVIDNYGCTDSIVKNNIVNIAQTIANFTTDTLVCPSTSIYFLNH
                     SSGNNLNYQWSYGDGNTANGFAVNHSYTNQGIYTVRLIATDDIGCADTLVRTNYVKVY
                     YPIANFTISDSTAVCPPLMVNTTNNSLYAGSTNWSFGNGSFSTNTNPSHLYIYPGNYT
                     IKLVVKNLGGCADSTTKNVIINGPTGTFSYPAVIACNPTQVKFKATSENSISNTWDFN
                     NGVTSKTIADSTAYTYTNGGHYVPKLILEDAAGCKVTIVGKDTIKVKYVQASFAPVSK
                     VVCDSSFIQFNDSS"
BASE COUNT         2689 a         1073 c         1236 g         2825 t
ORIGIN      
        1 gggggttata aagcttttcg taattatgca ctcaaatatt ttactaggga aagaaaaatt
       61 gttttattgg gcggaaaaac aggcagcgga aaaacagatg ttttaaaaaa attaaatgaa
      121 ctgtataaca aatggataga taactataaa gaaggtcctt tactagttat agattgcgac
      181 aaaaataaat ttgccgaaca tgatgaacat ttaggagaag taattagtaa agtagatagt
      241 atgttgtttg gtttatttta aaaaacgatg aagaaacttg gtttaacttt tttatttttc
      301 tctatttttc ttcttgctta tggtcaggat aagttagata gaaatactat tattggtaaa
      361 tgggttgcat ttggaatgct atataatgag gtatattata atttagatac tgactcattt
      421 tatatttcac cagaaaaata cgatttatta ttgagccaga atgaagatac aattggagca
      481 aaaaagtatt taagaaatct aatgctttcc atattgccct ttaactttaa ctttaaaaag
      541 aatggaaatt gcacctttga aaataaaaat atttttgaac gtaccaccta tgcctttgat
      601 gaattaaaaa aaataattac catttttaat gctacaacag gaaagttcat taatcttgaa
      661 gcatatatga aagataatga tttgtatata aatatgaata aggatgactc taattcaata
      721 aaagggtgta ttatttttaa gaaaataaaa taattttaat aattgtagtt atggtattat
      781 agttacccat tttaagctat tttcattttt taagtaattt aaaacgtttg ctatgcttac
      841 acttattcgc tggatggtag gtttactttt tattttttct ggtttaataa aagccaatga
      901 cccattgggt ttaagctaca aaatgcaaga attttttgaa gcttggcaac ttcatgcatt
      961 acataattat acattatttt ttgcttatgg tatgaatgtg ttagaagtag tggcaggcat
     1021 tgctgtaatt gttggatggc aaattaaaaa aacggtgtgg ttgcttttaa cgctaattgt
     1081 attttttact tttcttacag cttatgtatt gttttctggt aaaattaaag catgtggttg
     1141 ctttggagat tgtgtaccac ttacaccagt tcaaactttt actaaggata ttattttgtt
     1201 aattcttatt ttaatattgc tttttaaagt taatagtttt cgcagtagct taaataataa
     1261 cctatcattt gctattgttt tcattgcttt aattggcaca atattatttc aaaattatgt
     1321 gttgaaatat ttacctgttt tagattgttt gccttatgct aaaggaaaaa atttagttac
     1381 aggtatgcaa ctacctaagg gggctttgcc agatagtttt gctataactt ttaaatataa
     1441 aaaagatggc aaattaatag agtttgatca aaataatttt cctgcagatt ttgatgatac
     1501 ttatgaatat attgatagat acgataaatt agtaagaaaa ggaactgcat tgcctgctat
     1561 aacagatttt tctttaacta cagtttttgg caacgacact acacaggctt tattaaacag
     1621 caaaacaaaa tatattttat tatttgctaa agattttaat aatataaata aatgggataa
     1681 tgaagacttt aagaagggtg tagaaattgc aaaaaataaa aatatacctt tttatattgt
     1741 aactgctaat gtttcgtttg ctaaggaaca ttttaataac cttacaattc tttcttgcga
     1801 tgcagttgca attaaaacag cagcaagggt aaacccaact tactttttaa tgaatggagc
     1861 agatgtagat aataagataa gctatgcaaa taataaagaa cttttaaaag ctctaactac
     1921 attttaaaaa ttattaaaac tattaaaagt tcatgttcag ttttattatt aaaaaattgg
     1981 gttatggcat tttagtaatg tttggtgttg ttgtaatagt atttttttta tttcaaggct
     2041 ttggcgaccc atccagatta attataggtc aaacaggtga tagcacaacc ataaaaaata
     2101 ttagaaaaga actagcatta gatcagccta aatggaagca atttacaatg tacttgaatg
     2161 atataagccc attgggtttt tattcaaaaa cagaaagaaa agaaaaaaat attaaaggtt
     2221 attttgttgg gaatgttggt attaaactac cttatttaag aagaagttat caaaccaaaa
     2281 aaaatgtaag taccatttta agtgaggcta ttccacaaac cattgtattg gcatttgctg
     2341 caatgtttat tgcaatagtt tttggaattg ccttaggagt tgtagcagca ttaaaacaaa
     2401 atacctggtt agatactggt agtattttta caagtgtatt gggtatttcg gctccctctt
     2461 tttttatggg tattgttatt gcctatttat ttggttttgt tttaaataac tatactaatt
     2521 tgcctttaac aggaagttta tacgaaatag atgcttttaa tggtaaacaa ttacaactaa
     2581 aaaatttaat attacctgcc ataactttag gtattcggcc tatggcaatt attacccaaa
     2641 ttactcgtag tgcaatgtta gatgtgttaa atcaggatta tattagaaca gcttatgcaa
     2701 aaggactttc tactaaaatg gttgtttgga aacatgcttt aaagaatgca ttaaatccag
     2761 taattacatc tattacagga tggtttgcag aattgttagc tggggctttt tttgttgaat
     2821 atatttttgg gtggaatgga attggaaaac ttactgttat ggctttagaa aatttagact
     2881 ttcctgttgt tatgggcagt gttttactta cagcatcttt ttttatttta gtaaatatta
     2941 ttgcagattt attgtatagt tatattgatc caagaattaa acttagctaa ttttagttta
     3001 ttaggcaaca acctttaaac tgttttttat tttatttgct ttgtaagtaa ggtcactata
     3061 atctgaactt aagatggtta catgacccat ttttctgcca ggttttgtaa ttattttacc
     3121 atacaaatga acaaatacat tatccatact taaaatattt tctagcccat catatatggc
     3181 attgccatta tagttattgc tgccaattaa attaacaatt gctgatggca aaattgctga
     3241 agtgttacct aaaggaaaat ttaaaataat tcgccaaagc atatcatatt gactgctgta
     3301 attgccctca atggtatgat gcccactatt atgcactctt ggtgctgttt catttaccca
     3361 aatattatta ttgttatcta caaacaattc tactgcaaaa atgcctgggc tatttaagtt
     3421 tttaactaca cttaaagcaa cagcttctac tttataaact atattttctg gtaatttggc
     3481 tggactaaat tggtaatcta ataagtttaa ataaggatca aaaatcattt caactaaagg
     3541 gtacacagca gtttcttttt tatcattaac tgcaataata atagcaatct ctttagttat
     3601 tgtaatacat ttttctaaaa cagatggagc attaaaacct tttactgcat cttcttttgt
     3661 gtttattgtt tgtacacctt ttccatcata accaccttgg gcaattttat gaacagcagg
     3721 taataaattt acatggttta taatttcatt agcttgattg gttacaacaa attcagctgt
     3781 tggaatttga ttttgttgat aaaattgttt ttgtaaaatt ttattattaa ttgttttaat
     3841 tactgatgga gaagggtaaa tttttacacc ctctttttct aattgctcta aagcttcaac
     3901 attaactgat tctatttcta tggttaaagc atctactttt tttccgaaat tatatacagt
     3961 ttcaaaatct tttatatctc ctttaataaa atgggtacat aaatgtgcag aagggcaatt
     4021 ttcatcattc tctaaaacaa aagtttctac cacataattt gctgcagctt gtaacaacat
     4081 tcgtcctaac tgcccaccac ctaatattcc tacttttttt attgttgaat tgttgtttaa
     4141 agacataaag cttatttaaa aatatttaat gcacaaattg aaacatttgt tttcatatta
     4201 atacttttta aagtggattt tttaaaattt tctaaaaata acattcgtta agggtaaaat
     4261 ttttaataat ttttttggta attaaatttt ttaattaatt gattttcagg cttatttatt
     4321 aagagataga aattaaaatt attaaaatct ttttatcata caataaaaga gtatctaagg
     4381 tagttattat gtagcaaggt tttttatatc caatatccaa attccacact atgaaagcca
     4441 ttatggtttt gtacctgaaa tattttctgt gtacgagtat aattttactt tctttccttg
     4501 ttaaatccaa tgcacaattg gaggttggtt tttatgctaa tcatttaaca ggctgttctc
     4561 cagaattaat tcaatttaaa gatacttcaa aaggtaatcc aacttattgg aaatgggatt
     4621 taggtaatgg taccatttcc taccaaaaaa atccatcagc tacttacttc aatccaggtt
     4681 catacactat taaattattt attagcaatc aaaatggaga ggatagcatc actaaaacat
     4741 cttatataac tatttatgaa aaaccaactg taaatttcac tgccaataaa aatacaggtt
     4801 gtgccccttt acaaattcag tttaacgatt taagtgttgc tggcaatgat tctattacac
     4861 aatggctttg ggattttaat gatggtaata tttcttctct taaaaaccct aatcatattt
     4921 atacacaaca aggtgaattt gatgttaagc taaaagttac cactatgaat ggttgtttta
     4981 gtgcaataga aaaaaaggat tttattaaaa cagaaggttt gttacaagca ggctttaact
     5041 atactacttt aggagctaat tgtactgcac caacaactgt acaatttacc aatacatcaa
     5101 caggctcggg taatttaact ttcaattgga gctttggaga tggagctact tcaactatta
     5161 taaaccctac acatacttat tcaactacag gaagttttac tgtacagctt gttgttacta
     5221 atgctttagg atgtacagat acattaaaaa tagatcatgc aataactatt ggctcggtgc
     5281 aagcaaattt ttcattacca gattctattt gtgcaggcac caagctagat ttaatgaatg
     5341 cttcttcgcc aacagttgta tctaataatt ggtattttgg caatggctta aattcaactc
     5401 aaataaatcc ttccgtaact tttacaaatg ccggtactta tacaattaaa ttagttagca
     5461 attttggaac ttgtcttgat tctgtagaaa aatctattaa aatattatct aaacccgaag
     5521 ctaattttag tgcaataaat acttctggtt gttccttgcc tttacaaact cagtttacaa
     5581 attcatctat gggtggtgag tcttaccaat ggaattttgg aaatggtatt acttcaacct
     5641 tgcacagtcc cacccatact tttaccaatt ttggtattta caatgtaatg ttacaagtaa
     5701 cagcagaaaa tggttgtaca gatactttac taatggatag tgttgttaaa ataattccaa
     5761 taaaaattaa ttcaatagca attgacccag aagaaggttg tgtgccatta ttagtttctt
     5821 ttaatgctaa tattacggca gttgatagta ttgcaacgta cacttggtat tttggagata
     5881 atacaatatc caatgaaaaa gaaccaaagc atacgtataa ccaagctggt acttatgatg
     5941 ttaaacttgt tataacaagc actaatggtt gtgtagattc tttaattgta tttaatgctg
     6001 taaaagtagg agagaaacca agagcagatt ttgaggcaac tcctagagaa ggatgtacta
     6061 ttacaggtat caatttcacc gacctatcaa ccaatgcaac tgtagataaa tggtattgga
     6121 gctttggaga tgggaaaagg tcttataatc agaacccttt aacaacctat caagatactg
     6181 gttggtttaa tgtaagatta attgtatgga gtaatggctg ttctgatact atggttaaag
     6241 ctaattacat tcatattaaa cctcctaaag caaattttag ttttaaagca gaggattgta
     6301 ataataaatt aactgttcag ttttttgata gctcaaaagg tgcagtaaca tataaatggt
     6361 tttttggaga tggagatact tcaaacatag ctaacccaat tcacacttat tctaaatctg
     6421 gtacttatca agtattgtta gttgtagtaa atggcgattg ttctcattca agaataagaa
     6481 ctgttgtagt agatgataat aatggaaaac ttgttataag tgatactgta gtttgtagaa
     6541 atactaaagt gaatttttca ttaactaatt taaatacaat ttctgcagca acttatactt
     6601 ggagtgtagg agagggaaat ttaattacaa caggtggtgg ccctttaaca gaatatgtat
     6661 ataccaatgc tggtaaattt aatgtatggg ttgcaattga ttatgctaat ggctgtaaag
     6721 ataccattga atataacagc attaaagtat atggtgcaac agcaaaattt tcatcacccg
     6781 aaaatacatt ttgctctggt agtacaattg tatttaatga ttcttcattt acagatggaa
     6841 cacatgcaat aaccaactgg ttatggaact acggagatgg aactgttgaa aataataaca
     6901 atgcagcagc atcccatttt tatgcaacag gcggtagttt tgatgttaaa ttaaaagtaa
     6961 ttgataatta tggttgtacg gatagtattg tgaaaaataa tattgtaaat attgcccaaa
     7021 caattgcaaa ttttactaca gatactttgg tttgccctag tacaagtatt tatttcttaa
     7081 atcattctag cggtaataac ttaaactatc aatggagcta tggagatgga aatacagcaa
     7141 atggttttgc agttaaccat agttatacaa atcagggtat ttatactgtt agattaattg
     7201 caacagatga tattggttgt gccgatactt tggtaagaac aaattatgta aaagtatatt
     7261 acccaattgc aaattttacc attagcgatt ctacagcagt atgtcctcct ttaatggtaa
     7321 atactactaa caattctttg tatgcaggca gcacaaattg gagttttggt aatggaagtt
     7381 tttcaacaaa tactaaccca tctcatctct atatttatcc aggaaattat accattaaat
     7441 tagtggttaa aaatttagga ggctgtgccg attctacaac aaaaaatgta ataataaatg
     7501 ggccaacagg tactttttct taccctgcag taatagcttg taaccctact caggtaaagt
     7561 ttaaagcaac atccgaaaac agtatttcta acacatggga ttttaataat ggtgtaacct
     7621 ctaaaactat tgctgatagc actgcttaca cttatacaaa tggtgggcat tatgtaccaa
     7681 aattaatttt agaagatgct gcaggttgta aagtgacaat tgtaggtaag gataccatta
     7741 aagtaaaata tgtacaagca tcctttgcac ctgtttcaaa agttgtatgc gattcttctt
     7801 ttattcaatt taatgattct tca
//
LOCUS       DAOQPK010000089         7729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_5238_length_7729_cov_4.571671, whole genome shotgun sequence.
ACCESSION   DAOQPK010000089 DAOQPK010000000
VERSION     DAOQPK010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7729
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5238_length_7729_cov_4.571671"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            448..2010
                     /locus_tag="PLU36_05640"
     CDS             448..2010
                     /locus_tag="PLU36_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HMZ46267.1"
                     /translation="MRILTGLKNSALLVATLGILASCNSFKKKQQKSDVTGWAYNDKK
                     NGGFTVAKPKNIKTAPGLVFVQGGTFTMGATEEDVMGDWNNIPKRITVNSFFIDKTEV
                     ANVHYREYIFWMQNTFGEDDKYIQLMEDIKPDTLVWRSELSYNEPYVEYYFSHPSYNY
                     YPVVGVTWKQARDFCIWRTDRVNEKALIDKKIINAKDYAKGGMNNVGEDNFNTKAYLL
                     GLYNPKTPPSKGSPLKDSKGQPRQAKFDDGILYGDYRLPTEAEWEYAAYGYIMENPQK
                     RRNEKTRGEEVIGNKQVYAWRNAGYDNLRSTKRGAFQGAFLANFKRGSGDNMGVAGGL
                     NDRSAIPAEINSFYPNGFGIYNMSGNVSEWVADVYRPLTSLEGEDFNYYRGNVFQKVK
                     KDSSGEMQKDSLGRVVMVNEPDSVLRKRRNYQKSYAIDYLDGDQIADGRSAVYNYGTT
                     TLVSDKSRVVKGGSWNDRPYWLSPGARRFLEETQSSSTIGFRCAMTHYGGPESTKFGK
                     TGNNFPARRQKK"
     gene            2163..3392
                     /locus_tag="PLU36_05645"
     CDS             2163..3392
                     /locus_tag="PLU36_05645"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HMZ46268.1"
                     /translation="MAVKIKVANPIVELDGDEMTRIIWKFIKDKLILPYIEVDIKYYD
                     LGVEYRDATNDQVTIDAANAIKQYGVGIKCATITPDEQRVEEFKLKQMWKSPNGTIRN
                     ILDGTVFREPIVCNNVPRLVPNWTAPICIGRHAFGDQYRATDFVTKGKGKLTIKFEGE
                     DGNVIEHEVFNFKSDGVALAMYNTDESIIGFARSCFNTALQKKWPLYLSTKNTILKKY
                     DGRFKDIFEEIYQQEFKSKFEAAGITYEHRLIDDMVASALKWNGNFVWACKNYDGDVQ
                     SDTVAQGFGSLGLMTSTLVTPDGKIMEAEAAHGTVTRHYREHQKGRPTSTNPIASIFA
                     WTRGLEFRGKLDNNNDLIKFAHALEQVCIETVESGKMTKDLAVCIHGNNVKHGEHYLY
                     TEEFLDALDNALQAKLA"
     gene            3501..3962
                     /locus_tag="PLU36_05650"
     CDS             3501..3962
                     /locus_tag="PLU36_05650"
                     /inference="COORDINATES: protein motif:HMM:NF016535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMZ46269.1"
                     /translation="MAVKTFLLLMACFLAGALTPIQAGFNFRLAKAVNNTISAALISF
                     LVGTVILLAYLAFTKQLKLDLVTVAKTEPFWVWLGGVIGAFFVVTLTFVVPKVGASLS
                     FSLIIAGQLIISIIIDHYGLLGVPVTPINMKRIAGILLLALAIWLIKEKNV"
     gene            complement(4035..5717)
                     /locus_tag="PLU36_05655"
     CDS             complement(4035..5717)
                     /locus_tag="PLU36_05655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HMZ46270.1"
                     /translation="MKYLISTISILLTSFTTVFAQQTTSTDSLYITQDSILIPTKSGI
                     DISAIIVRKKTNVNPLPAILFYTTYYQGAGDKIFGIRSADRDYVGIVAYARGIKTNLK
                     NYIPFENEGTDIYDIIDWISKQSWCNGSVGMFGGSYTGFSQWATAKNLHPALKTIVPQ
                     VAVIPGFDFPMENNVPLSHILSWSNDNIYKSKLLPRSLPFDYYNSGVAFNKMDSLAGY
                     KNPIFQNWLKHPAYDSYWQSMVPTSVEYAKINIPVLSTTGYYDGSQIGAIQYFKLHNK
                     YNKNANHYFVIGPYDHWGAQRKPAKNLMGYEIDSIANINMMNLAYDWLDYVLKGRPKP
                     ALLKDKINYQVMGTNEWKHSSTLDKINNDTLTFYLDKTRLSLTKPQKKEFQKQTVDFN
                     DRENQNNYYTPEIIFDTLDASNGLVFTTDIFQKEYIVNGSFSGNLWVSINKKDMDVSM
                     ALYEKMENGKYFFLTRYVGRASFAKDNSKRQLLQPNKKENIPFNNTRFVSKKINKGSR
                     LVILLNINKHPFEIINYGSGKPVSEETIKDAGKPLQIKWHNDSFIKIPIWKG"
     gene            complement(5991..6425)
                     /locus_tag="PLU36_05660"
     CDS             complement(5991..6425)
                     /locus_tag="PLU36_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46271.1"
                     /translation="MKSKINYILILLFSLTACNKDNTTTTSDSLNGKWKLVKYYNLTT
                     GTIESEPSNISRSIILELSDNRIQGNISGHTVTNSVSGEYELLKDNKMKTLSFGGTKI
                     GEPNWGDKFWDAIHATSSYERQSDKLYIYFNADMEKMEFKKL"
     gene            complement(6544..7062)
                     /locus_tag="PLU36_05665"
     CDS             complement(6544..7062)
                     /locus_tag="PLU36_05665"
                     /inference="COORDINATES: protein motif:HMM:NF026080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4468 domain-containing protein"
                     /protein_id="HMZ46272.1"
                     /translation="MTIPSFGQNEKLFDILPIKEGKVIYTDVVIVDSTDKVKLYSKVK
                     KWFIEQYKSAKDVIQLDDKENGEVIGKGFFETNWQNSFISELKVNVWHTIKIYIKDNR
                     FKYEITNFTLKYYVGGTRYSTGSTVEEAIEDFGKDRVRNTKNYFVTVDSEIVSIISSL
                     INFMKAPNKEDW"
     gene            complement(7441..>7729)
                     /locus_tag="PLU36_05670"
     CDS             complement(7441..>7729)
                     /locus_tag="PLU36_05670"
                     /inference="COORDINATES: protein motif:HMM:NF022902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46273.1"
                     /translation="KKNQSLIVSDILKGQGKFAAEWMLVAQKVEKNARWILKPMNFCM
                     NYFGNGEIEITNRGNFRIGRITMQRKGGDGGRDTAKMLQFKINPAELFDNE"
BASE COUNT         2590 a         1132 c         1272 g         2735 t
ORIGIN      
        1 tacaatatgt gttgatgttc gttatgtatt atggggtata gaaaaagtag gtggggcaaa
       61 acacattatt gacgaactta ctttagttaa ataaaataca ttttaaagta attgtagcta
      121 aaaccctgaa accatttcag ggttttttta tacgtatgta atcattttta ttaaaaactt
      181 aacctcaaat attatagagt ctaaaaaata agtgattgac aatcaatact tttttcgaaa
      241 ttaaaattat accatttcgc caaaattatt tacagggaaa gtagtatttt tttaaaattt
      301 ttgcttgtaa atcaatgcta aataaatttg ttaaattatt tttagttgta gtttagaaat
      361 aattcaataa ttacttttat attcgcaact cttaaataga caatattttt ttaaaatcaa
      421 cgttttagta cactcaaact ttcaaaaatg cgaatcttaa cagggcttaa aaattctgca
      481 ttgctagttg caacacttgg catcttagca tcttgtaatt cttttaaaaa gaaacaacaa
      541 aagtcagatg taaccggctg ggcttacaat gataaaaaga atggaggctt tacggtagca
      601 aagccaaaaa atattaaaac agcacctgga ctggtgtttg ttcaaggtgg tacatttaca
      661 atgggtgcta ctgaagaaga tgtaatggga gattggaata atatcccaaa acgtattaca
      721 gtaaactcgt tctttattga taaaacagaa gtagcgaatg tacactatcg tgagtatatt
      781 ttttggatgc aaaatacctt tggggaagat gataaatata ttcaattaat ggaagatatt
      841 aaaccagata cccttgtttg gagaagtgaa ttatcttata atgaaccata cgttgagtat
      901 tattttagcc acccaagtta taactattat cctgtagtag gtgttacttg gaaacaagct
      961 cgtgattttt gtatttggcg taccgataga gttaacgaaa aagctttaat tgataaaaaa
     1021 atcattaatg caaaagatta tgccaaaggc ggcatgaata atgttggcga agataacttt
     1081 aatacaaaag catatttatt aggcttatat aatcctaaaa cacctccaag caaaggctct
     1141 cctttaaaag attctaaagg ccaacctcgt caggctaaat ttgatgatgg tattttatat
     1201 ggagattatc gtttaccaac tgaagctgaa tgggaatatg cagcttatgg ctacattatg
     1261 gaaaaccctc aaaaacgtag aaatgaaaaa accagaggcg aagaagttat aggtaacaaa
     1321 caagtttatg cttggagaaa tgcaggttac gacaacttac gttctacaaa aagaggtgct
     1381 tttcaagggg cattcttagc aaactttaaa agaggttctg gcgataatat gggagttgca
     1441 ggtggtttaa atgaccgttc tgcaatacct gcagaaatta attcttttta tcctaatggt
     1501 tttggaattt ataatatgag tggtaacgta agtgagtggg ttgccgatgt ttacagacca
     1561 ttaacaagtt tagaaggaga agattttaac tattatagag gtaacgtttt ccaaaaagtt
     1621 aaaaaagatt catctggcga aatgcaaaaa gattcattag gaagagtagt tatggttaac
     1681 gagccagata gcgtattaag aaaacgcaga aactaccaaa aatcttatgc tatagattat
     1741 ttagatggag accaaatagc tgatggtaga agtgcagttt ataactatgg tacaacaaca
     1801 ttagtttctg ataaatctag agtagtaaag ggaggtagtt ggaatgatag accatattgg
     1861 ctaagcccag gtgcaagaag atttttagaa gaaacacaaa gcagcagtac cattggtttc
     1921 cgttgtgcaa tgacacatta tggcggtcca gaaagtacta agtttggaaa aactggaaac
     1981 aattttccag caagaagaca aaagaaataa gggaaaggta aatacagctt tcttgtttta
     2041 aacaggaata aaataaagtc actgattttt ttagtggctt tttttatatt attatttaag
     2101 attctaagtg caatctattt atatactaca tttgcaaacc tttaaaaata aattataata
     2161 ttatggcagt taaaattaaa gttgctaatc caatagtaga attagatggc gacgagatga
     2221 cacgaataat ttggaaattt attaaagaca aattaatact tccatacatt gaagttgaca
     2281 ttaaatatta cgatttagga gttgaatata gagatgcaac taacgaccaa gtaacaattg
     2341 atgcagccaa tgcaataaaa cagtatggtg ttggtattaa atgtgcaact attacaccag
     2401 atgaacaaag ggttgaagaa tttaagttaa aacaaatgtg gaagagcccc aatggtacta
     2461 ttagaaatat tttagatggt acggtttttc gtgagccaat tgtttgcaac aatgtacctc
     2521 gtttagttcc aaactggaca gcaccaattt gtattggccg acatgctttt ggagaccaat
     2581 atagagcaac agattttgta accaaaggaa aaggtaaatt aaccattaag tttgaaggag
     2641 aagacgggaa tgtaatagaa catgaagtat ttaactttaa aagcgatggt gttgcattag
     2701 ccatgtataa tacagatgaa agcattattg gctttgccag aagttgcttt aatactgctt
     2761 tacaaaaaaa atggccatta tatttaagta ctaaaaatac tattcttaaa aaatatgatg
     2821 gcaggtttaa agatattttt gaagaaattt atcagcaaga atttaaatca aaatttgaag
     2881 cagcaggcat tacttacgag catcgtttaa ttgatgatat ggttgctagt gctttaaaat
     2941 ggaatggcaa ttttgtatgg gcttgtaaaa attatgatgg agatgtacaa agcgatacag
     3001 tagctcaagg ttttggtagt ttaggtttaa tgacatctac attagttacc cctgatggaa
     3061 agattatgga agcagaagct gcacatggaa cagttacaag gcattaccgt gagcatcaaa
     3121 aaggcagacc aacttctacc aaccctattg catctatttt tgcatggacg aggggtttag
     3181 agtttagagg taaattagac aacaacaatg atttaataaa atttgctcat gctttggaac
     3241 aagtttgtat agaaactgtt gaaagtggta aaatgacaaa agatttagca gtttgtattc
     3301 atggtaataa tgtaaaacat ggagaacatt atttatatac cgaagaattt ttagatgctt
     3361 tagataacgc attacaagcc aaattagctt aatagtagat aactatttta taaaactgcc
     3421 gataaattta cggcagtttt tttattgttt tttttaaaac taatttttaa aattataatt
     3481 ttagcaactt atataaaatt atggcagtaa aaactttttt gcttttaatg gcttgctttt
     3541 tagcaggagc attaacacca atacaggctg ggtttaactt taggttagca aaggcagtta
     3601 ataatactat aagtgcagct ttaatttctt ttttagttgg cactgtaatt ttattggctt
     3661 atttagcttt caccaaacaa ttaaaacttg atttagttac agttgccaaa actgaacctt
     3721 tttgggtttg gttaggcggt gtaattggag ctttttttgt tgttacatta acctttgtag
     3781 ttccaaaagt tggggcatca ttatctttta gtttaatcat tgcagggcaa ttaattattt
     3841 caataattat tgatcattat ggattattag gtgtgcctgt aaccccaata aatatgaaaa
     3901 gaattgctgg tattttatta ttagctcttg caatttggct aataaaagaa aagaatgttt
     3961 aatttttttt gtgaaagaaa aataaatcac agtgtaactt ttgttgtata aataatagca
     4021 agttttttag cgtactatcc tttccaaatt ggaattttaa taaaactatc attgtgccat
     4081 tttatttgta atggttttcc agcatctttt attgtttctt cagatacagg tttacctgaa
     4141 ccataattta ttatttcaaa aggatgtttg ttaatgttta gcaatataac aagcctgcta
     4201 cctttgttaa ttttcttact tacaaaacgg gtgttgttaa aaggaatatt ttcttttttg
     4261 tttggttgta gtagttgtcg tttagaattg tcttttgcaa aacttgctct accaacatat
     4321 cttgttagaa aaaaatattt accattttcc attttttcat aaagtgccat tgaaacgtcc
     4381 atatcttttt tatttatact tacccaaaga tttcctgaaa aagagccatt aacaatatat
     4441 tctttttgaa aaatatcagt tgtaaaaact aatccattgc ttgcatcaag tgtgtcaaaa
     4501 ataatttcag gagtataata attattttga ttctctctgt cgttaaagtc aacagtttgt
     4561 ttttgaaatt cttttttttg gggcttggtt aatgatagtc ttgttttatc tagatagaaa
     4621 gttaaagtgt cgttgttaat tttatcaagt gttgaactat gtttccattc gtttgttccc
     4681 attacttgat agttaatttt gtctttcaac agtgcaggtt ttggcctacc tttaagtaca
     4741 taatcaagcc aatcgtaagc caaattcatc atattaatat tggcaatact atcaatttca
     4801 taacccatta agtttttagc aggtttgcgt tgtgcacccc aatgatcata agggccaatt
     4861 acaaaataat gatttgcatt tttattgtat ttattatgta gtttgaaata ttgaatagct
     4921 cctatttgag aaccatcata ataccctgtt gttgataaaa caggaatatt gattttagca
     4981 tattcgactg atgttggtac cattgattgc caataactat cataagcagg gtgctttaac
     5041 caattttgga aaattggatt tttataacct gctaaactgt ccattttatt gaatgctaca
     5101 ccactattgt aatagtcaaa aggtaaactt ctgggaagaa gtttgctttt ataaatattg
     5161 tcattactcc aacttaatat atgagataat ggaacattat tttccattgg aaaatcaaaa
     5221 ccaggtatta cagcaacttg tggtacaatt gttttcaaag cagggtgcaa gtttttagca
     5281 gtagcccatt gtgaaaatcc agtgtaactt ccaccaaaca ttccaacact tccattacac
     5341 caactttgtt tacttatcca atcaataatg tcgtaaatat ctgttccttc gttttcaaag
     5401 ggtatgtaat ttttcaagtt agttttaatg ccccgagcat aagctacaat gcctacataa
     5461 tctctatctg cagaacgaat tccaaaaatt ttatcacctg ctccttgata ataagttgta
     5521 taaaataaaa ttgcaggtaa tggattaaca tttgtttttt tacgaactat tattgcggaa
     5581 atgtcaatgc cactttttgt aggaattaat atgctgtctt gagttatgta taaactatct
     5641 gtagaggtgg tttgttgagc aaaaactgtt gtaaaacttg tcagtaaaat acttattgta
     5701 ctgattaaat atttcatact tattttcctg ttttggaagt tcattcttgt agtgtactat
     5761 ttttatttaa aagtaatgtt ttacaatctg atacttgata atagaatggg atgtaaaact
     5821 aaaatttatt agaagaataa aagatgaatt ttgcacaaaa aaccaatcaa aaccgttaag
     5881 ccctgctttt gcaaatatgt ttgttggcgg tagttttttg tttcttgtac ttgcatcatg
     5941 tctgtttttt caataagtca aagcagtagc tgtaattact tttagcaatc ttataatttt
     6001 ttaaattcca ttttctccat atcagcatta aaataaatgt aaagcttatc actttgcctc
     6061 tcataagacg aagttgcatg tattgcatcc caaaatttgt caccccaatt tggttcacca
     6121 atttttgttc caccaaaact taatgttttc attttgttgt ccttcaacaa ttcgtattct
     6181 ccgctaacag aattcgtcac agtgtgtcca cttatatttc cttggattct gttgtcagac
     6241 aactcaagaa taattgaacg agaaatattt gatggttcgg attcaatagt tccagtcgtt
     6301 aagttgtaat atttaaccag tttccattta ccgtttaaag agtcagatgt agtggttgtg
     6361 ttatccttgt tacaggcagt caaactaaaa agcagaatta aaatgtaatt tattttactt
     6421 ttcattgttt tggaatttat tatattagtt taaagtaatg taattattgt tccccgttgt
     6481 aaagttaaat ggttacgata aaattaccgc caacacaggg ctttatgcag gttgaagtgc
     6541 ttactaccag tcttctttat taggtgcttt catgaaattt atcaatgacg aaataattga
     6601 aactatttct gagtctactg tgacaaaata atttttcgta tttcttactc tgtcttttcc
     6661 aaaatcctca attgcttctt ctacagttga acctgtagaa taccgagtac cacctacata
     6721 atattttaga gtgaaatttg taatttcata tttgaaccta ttgtccttta tgtatatttt
     6781 tatagtgtgc caaacattta ccttcagttc agatataaat gaattctgcc aattagtttc
     6841 aaaaaaacct tttccaatta cttctccatt ctctttgtcg tccaattgta ttacatcttt
     6901 tgcagactta tattgttcaa taaaccactt tttcacttta gagtaaagtt tgactttatc
     6961 agtactatct acaattacaa catctgtgta aattacttta ccttctttta taggaagaat
     7021 atcgaatagt ttttcatttt gcccaaatga tggaattgtc aatatcagaa aaatactcaa
     7081 taaagtttta cacataagat aggtcagttt atatttctat gaacccatat ttccgacaac
     7141 tccgtaagtt tttggaagtg gcttacaaaa cttgttttga taaggcatgg gagcaatcac
     7201 ttttaatctc tgctattttt caaacaaggt gtaaggctaa attagcaaaa aagtatttgt
     7261 gaagcaacat tttgcttcaa acgaatgaga gtggtattat gtcttatggt ttgtgtatta
     7321 cagcagttga caatttctga aaacttcaca gttaacctgc actaaaaatt ttttgttgca
     7381 atacctctaa aattggcact aaccacccaa ttgatttgag ccattattat agtgcgtatt
     7441 ttattcattg tcaaataatt cagctggatt aattttgaat tgcaacatct ttgcagtatc
     7501 tcttcctcca tcaccacctt ttctttgcat tgtaattctt ccaattctaa aatttcctct
     7561 attagtaatt tcaatttctc cgttcccgaa atagttcata caaaaattca ttggtttaag
     7621 aatccacctt gcatttttct caactttttg agcgactaac atccattctg ctgcaaattt
     7681 cccctgtccc ttcaatatgt ctgaaacaat taaagattga tttttcttt
//
LOCUS       DAOQPK010000090         7555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_5432_length_7555_cov_4.529867, whole genome shotgun sequence.
ACCESSION   DAOQPK010000090 DAOQPK010000000
VERSION     DAOQPK010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7555
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5432_length_7555_cov_4.529867"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..924)
                     /locus_tag="PLU36_05675"
     CDS             complement(49..924)
                     /locus_tag="PLU36_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HMZ46274.1"
                     /translation="MNVLFYIACMNSIKTICVCGAGTMGSGIAQVTAQAGFKTIQFDV
                     NENMLDKSKIAIEKSLSFLVNKEKITEENKQAILQNLSFTSDIKECKADLIIEAIIEK
                     PEAKINLFNQLSIVNNTFTIYATNTSSISINEIANACSFKENVVGMHFFNPATIMKLV
                     EVVKANTTSHATVSSIVAVCKQMNKVPVVCKDAPGFIVNRVARHYYLEAMKIVEQNMA
                     TIENIDCLLESTGFKMGPFKLMDLIGMDINYSVSNIVWEALNKPERLKPSTLQQQKVL
                     AGELGRKTGKGFYKY"
     gene            1000..1641
                     /locus_tag="PLU36_05680"
     CDS             1000..1641
                     /locus_tag="PLU36_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HMZ46275.1"
                     /translation="MAKNKTLKQLDLFAAFEPTPATVNEIEANLISAKQVVFANDKIG
                     VKLKSKNTTVTSDNSIIKKQTSPEIQIEKNLTTKNKRGRKSFKEMDAEIDLVNVPSDE
                     ILKKKLYYPIREVAAFFNVNASLIRVWETEFDILQPRKNRKGDRLFRVEDIKNIQVIY
                     YLLRNRKFSLDGAKKYLKENKGKLTTNQQLIQSLIQLKSFLTEIKTSLLVKNN"
     gene            1717..2262
                     /locus_tag="PLU36_05685"
     CDS             1717..2262
                     /locus_tag="PLU36_05685"
                     /inference="COORDINATES: protein motif:HMM:NF025946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMZ46276.1"
                     /translation="MKNIFYILFICVLMISCKASFLKELSTVNYETSRDEGHLDNHKI
                     LKGTINRNILLQDTAFKWFTDNYQYANPDATAVDAFNKNKDKFKVLVFAGMWCEDSRN
                     LLPLFYKLTDNSNFSEDNIYLIGVDRAKTTINNLHTKFNITHIPTFIILHKNKEIGRV
                     VEYGRTGMIDKELGAIVKPIK"
     gene            2378..3454
                     /gene="gcvT"
                     /locus_tag="PLU36_05690"
     CDS             2378..3454
                     /gene="gcvT"
                     /locus_tag="PLU36_05690"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HMZ46277.1"
                     /translation="MKTTPFTQKHIALGAKMVEFAGYNMPISYTSINEEHEAVRTAAG
                     VFDVSHMGEFILKGEHALDLIQRTTTNDASKLTAGKAQYSCLTNEQGGIVDDLIIYCI
                     EENKVYLLVVNASNIEKDWNWISKFNTNNVEMHNISAKTCLLAIQGPTAVQLLQPLVK
                     SDILNLKYYTFVKDNFAGVDNVLISATGYTGAGGVEIYFEDTNGAADKIWDTILSLGI
                     KPIGLATRDTLRLEMGYCLYGNDIDDTTSPLEAGLGWITKFSKEFTAKAIIEKQKADG
                     IKKKLVGFEMLERGIPRHNYIIKDASGNTIGNVTSGTQAPSLGKAIGLGYVNIANAAI
                     DSEIFIDIRNNPVKAKVVKLPFSK"
     gene            complement(3504..3944)
                     /locus_tag="PLU36_05695"
     CDS             complement(3504..3944)
                     /locus_tag="PLU36_05695"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HMZ46278.1"
                     /translation="MKQIFFSILLMLFVVGCNYNSRIEENEMKIIVWEISCVDELINH
                     THLKDQDTSKKYTAQRDSLYQKIFAMHNTTADKFYKTFSYYEENPIQFKLLLDSALAY
                     GIRQRSNVIQTVKETIPTQPIQTTGTTTKDSLRKLTLKRNKALE"
     gene            complement(3949..4872)
                     /locus_tag="PLU36_05700"
     CDS             complement(3949..4872)
                     /locus_tag="PLU36_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HMZ46279.1"
                     /translation="MNYKLIIIFLLSFSCFSQLNAQELQAKVTVIANRIPTTVDKKIF
                     TTLQTQLNNFMNNRKWSNDVYKANEKIVCNFLLNLESMEDVNIYKASLTIQSARPVYN
                     SAYQAALINYMDADVTFKYVEYQPIEFNENRVQGTDALAANLTAVFAYYAYMILGLDY
                     DSFSPKGGQAFFMKAQNIVNNAPEAKGISGWRVFDGLRNRYFLVENLINSRYNILHDV
                     IYSYYRNGLDKMYEAEKESRSKILQTLHQLVSFNKENANTMFLQFFMQNKSAELIGIF
                     KNGTPEEKHQAIEALSLIDITNTAKYKEQLR"
     gene            complement(4872..6062)
                     /gene="coaBC"
                     /locus_tag="PLU36_05705"
     CDS             complement(4872..6062)
                     /gene="coaBC"
                     /locus_tag="PLU36_05705"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HMZ46280.1"
                     /translation="MLQDKKILIGITGSIAAYKTILLVRMLVKAGADVKIVITPSAKD
                     FVSPLVLSTLSKNKTISELSTNDSWENHVMLGRWADVFLIAPLSCNTLAKMANGICDN
                     VLMAIYLSATCPVVVAPAMDEDMWKHAATKRNMATLKKDSVQFIDVNNGELASGLIGE
                     GRMAEPEEIFTFLIENFFRTTEFVGKKILITAGGTIEAIDPVRFISNHSTGKMGFAIA
                     EAFYLQGAEVIIVAGNVQVTTKYKGIKVVSVTSADEMYKATLQYFKLSNIAVLSAAVA
                     DYKPITTAKEKIKKKEQILSLNLIKNKDILATLGNTKKRNQFVVGFALETNNENENAL
                     NKLKSKKVDVIILNSLKQKGAGFKTNTNQISIYNKNGSVINFDLKTKAAVANDIVKYI
                     KNSI"
     gene            complement(6139..6480)
                     /locus_tag="PLU36_05710"
     CDS             complement(6139..6480)
                     /locus_tag="PLU36_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HMZ46281.1"
                     /translation="MSKLRRQLSANTVSVIETKNLADIKAVTGNLYESISIIGKRANQ
                     INVSLKEELKNKLEEFASHTDNLEEVHENKEQIEISRAYEKMPNAAILATQEFLENKI
                     YYRNNDEDLFR"
     gene            complement(6455..7273)
                     /gene="bamD"
                     /locus_tag="PLU36_05715"
     CDS             complement(6455..7273)
                     /gene="bamD"
                     /locus_tag="PLU36_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HMZ46282.1"
                     /translation="MKRVLLFLLFTIVLGACSNKFSKIQKSKDYQYKLKMAEQYYAKK
                     KYRFAQILFEEVFPYVKGTAEFEDTYYKFAYCYYYDKDYLNAENLFKNFVETFPNSNK
                     AEECEYMRAFCYYKQSPKVDLDQTNTNKAIGLMQAFINKHPGSARVKDASNIIDLCRE
                     KLELKRYKSAELYYNLGFYKAAAICFSNVSENFPDSKKADEYKLLVIKSYYKYAEMSI
                     PAKQEERFEMVIKECKDFTDRFQDSKYFAEAEKYKNLSTNFIKNIKNEQVKKAA"
BASE COUNT         2595 a         1116 c         1032 g         2812 t
ORIGIN      
        1 atttatcgta tagtttgtat aaaaactact ctcagaagat tttgcttttt agtatttgta
       61 aaatccctta cctgtttttc tacccaattc tccagctaaa actttttgtt gctgtaaagt
      121 agaaggtttt aaacgttctg gtttgtttaa tgcttcccaa acaatattac ttacactgta
      181 gttaatatcc atgcctatta aatccatcaa tttaaaaggg cccattttaa aaccagttga
      241 ttctaataaa caatctatat tttcaattgt agccatattt tgttctacta ttttcattgc
      301 ttctaaataa taatgtctgg caaccctatt tacaataaaa cctggggcat ctttacaaac
      361 tacaggtact ttgttcattt gtttacaaac tgcaacaata ctactaactg tagcatggct
      421 tgtagtgttt gctttaacaa cctcaactaa tttcattatg gtggctgggt taaaaaaatg
      481 catacctaca acattttctt taaaagaaca tgcatttgca atttcattaa tactaataga
      541 agaagtattt gtggcataaa ttgtaaatgt gttatttaca attgataact gattaaataa
      601 gttaatcttt gcttctggtt tttcaatgat tgcttcaata attaaatctg ctttacattc
      661 cttaatatct gaagtgaaag ataaattctg gaggattgcc tgtttgtttt cttcggttat
      721 tttttcttta ttaactaaaa aactcaggct tttctcaata gcaattttgc ttttatctaa
      781 catgttttca tttacatcaa attgaattgt tttaaagcca gcttgagcag taacctgtgc
      841 aattccacta cccattgtac cagcaccaca aacgcaaatt gtttttatac tattcatgca
      901 tgcaatataa aataatacat tcatatttgt tgtttacatt ttatacacca ttaaattgca
      961 tacttaatta ataaccacag catttagtat ttttacataa tggcaaaaaa taaaacttta
     1021 aagcaattag atttatttgc agcttttgaa cctacacctg ctactgtaaa tgaaatagaa
     1081 gctaacttaa tttcagcaaa acaagtagtt tttgcaaatg ataaaattgg tgttaaatta
     1141 aaaagcaaaa acactactgt aacttctgat aattcaataa taaaaaagca aacttctccg
     1201 gaaatacaaa tagaaaaaaa tcttactact aaaaataaaa gaggaaggaa aagttttaaa
     1261 gaaatggatg ctgaaattga tttagttaat gtaccaagcg atgaaatatt aaaaaagaaa
     1321 ttgtattatc caataagaga agttgctgca ttttttaatg taaatgcttc tttaataaga
     1381 gtttgggaaa cagagtttga tattttacaa cctcgaaaaa atagaaaagg cgatagatta
     1441 tttagagtgg aagatataaa aaatattcag gttatttatt atttactaag gaacagaaaa
     1501 ttctctttag atggtgccaa aaaatattta aaagaaaata aaggtaaact taccactaat
     1561 cagcaactta ttcaatctct tattcaactt aaatcatttt taaccgaaat taaaacgagt
     1621 ttattagtta aaaataatta atgaatgctt tcattaattc aaattaattt aaaataactt
     1681 cagatactta aaacatttta agcataaaca aaaattatga agaatatatt ttacatctta
     1741 tttatttgtg ttctaatgat ttcctgtaaa gcttcttttt taaaagaact ttcaactgta
     1801 aattatgaaa ctagtagaga tgaaggccat ttagataacc ataaaatatt aaagggcaca
     1861 attaatagaa atattttatt acaagacact gcttttaagt ggtttacaga taattaccaa
     1921 tatgccaatc ctgatgccac agcagttgat gcttttaata agaacaaaga caaatttaaa
     1981 gtattagttt ttgcaggaat gtggtgcgaa gacagtagaa acttattgcc tttattttat
     2041 aaattaacag ataatagtaa tttttctgaa gataatattt atttaattgg tgtggataga
     2101 gccaaaacca caataaataa tttacatact aaattcaata ttactcatat tccaactttt
     2161 attattctgc acaaaaacaa agaaataggc agagtggttg aatatggcag aacaggaatg
     2221 atagataaag aattaggtgc aattgttaaa ccaataaagt aattaatatt cttgaattta
     2281 ttttccaact ctattaatga aattataatt tctaattttc agtatttgtt atagtacatt
     2341 tgccaaaatt tttttgaata ctttaaatat ttttacaatg aagacaactc catttacaca
     2401 aaaacatata gcattaggtg caaaaatggt tgaatttgca ggctataata tgcctatttc
     2461 ttatacaagt attaacgaag aacatgaagc tgtacgaact gcagcaggag tttttgatgt
     2521 aagccatatg ggtgaattta ttttaaaagg cgaacatgct ttagatttaa ttcaacgtac
     2581 aacaactaat gatgcttcta aacttacagc aggaaaagca cagtatagtt gccttactaa
     2641 cgaacaagga ggtattgttg atgatttaat tatatactgc attgaagaaa ataaagttta
     2701 cttattggtt gtaaatgcta gtaatattga aaaagattgg aattggataa gtaaatttaa
     2761 tactaataat gttgagatgc acaacataag tgctaaaaca tgtttattgg caatacaagg
     2821 cccaactgct gtacaattat tacaaccatt agttaaatct gatattttaa atttaaagta
     2881 ttacacattt gtaaaagata attttgctgg tgtagataat gttttaatta gtgctacagg
     2941 ttatacaggt gctggtggag tagaaattta ttttgaagat acaaatgggg cagcagataa
     3001 aatttgggat accattttaa gccttggtat aaaaccaatt ggattagcta caagagatac
     3061 tttaaggtta gaaatgggct actgcttata tggaaacgat atagacgata ccacttctcc
     3121 attagaagct ggccttggct ggataacaaa atttagcaaa gagtttactg caaaagcaat
     3181 tatagaaaaa caaaaggccg atggtataaa aaagaaatta gttggctttg aaatgttaga
     3241 gagaggtatt cctcgacaca attatattat taaagatgca tctggaaata caataggtaa
     3301 tgttacaagt ggtacacagg caccaagctt aggcaaagct attggcttag gttatgtaaa
     3361 cattgctaat gctgcaattg attctgaaat atttatagat attagaaata atcctgtaaa
     3421 agcaaaagtt gtaaaactac ccttttcaaa ataattaaga gctaagatat aatatataat
     3481 atatgcagta aggcgtttga taattattct aacgccttat ttctttttaa tgttaatttt
     3541 cttaaactat ctttagttgt agtacctgta gtttgtatag gttgtgtagg tatggtttct
     3601 ttaactgtct gtattacatt acttctctgc cttattccat aagccaatgc agaatctaaa
     3661 agtagtttaa attggattgg attctcttca taataactaa aagttttata aaatttatct
     3721 gcagttgtat tatgcattgc aaatattttt tgatataaag aatctctctg tgcagtatat
     3781 tttttggaag tgtcttgatc ttttaaatgt gtatgattaa ttaactcatc aacacaagat
     3841 atttcccaaa caattatttt catttcattt tcttctattc tgctattgta attacaacca
     3901 acaacaaaca gcattaataa gatagaaaaa aatatttgct tcattttgtt accttagttg
     3961 ttctttatat tttgctgtgt tggttatatc aattaaacta agtgcttcaa ttgcttggtg
     4021 tttttcttcg ggagtgccat ttttaaaaat acctattaat tctgccgatt tattctgcat
     4081 aaaaaattgc aaaaacatag tattagcatt ttctttatta aaactaacta gttgatgtaa
     4141 ggtttgcaat attttacttc tagattcttt ttcagcttca tacattttat ctaatccatt
     4201 tctataataa ctgtaaataa catcatgcag aatattatac ctgctattaa ttaaattttc
     4261 aactaaaaaa tacctgtttc ttaagccatc aaaaactctc caaccagata ttcctttagc
     4321 ctctggagca ttgtttacaa tattttgagc tttcataaaa aatgcttgcc caccttttgg
     4381 agaaaaggaa tcgtaatcta agcctaatat catataagca taataagcaa aaactgcagt
     4441 taaattagct gccaatgcat cggtaccttg taccctattt tcattaaact ctattggctg
     4501 gtattctaca tatttaaaag ttacatcagc atccatataa tttattaaag cagcttgata
     4561 ggcagagtta tacactggtc tggctgattg aattgttaga gatgctttgt agatatttac
     4621 atcttccatg ctctctaaat tcaacaaaaa attacaaaca attttttcat ttgctttata
     4681 aacatcatta ctccatttac ggttgttcat aaagttattg agctgtgttt gcaacgtagt
     4741 aaatattttt ttatctacag tagttggtat tctatttgct attacagtaa cttttgcttg
     4801 taactcctgt gcattcaatt gggaaaaaca agaaaaagat agtaggaaaa ttatgattaa
     4861 tttgtaattc attaaattga attttttata tactttacaa tgtcattagc tacagctgct
     4921 tttgtcttta aatcaaaatt aattacagac ccatttttgt tataaatgct aatttggttt
     4981 gtgtttgttt taaaacctgc acctttttgt tttaacgaat ttaatataat aacatctact
     5041 ttttttgatt ttaacttatt taatgcattt tcattttcgt tattggtttc taaagcaaag
     5101 ccaacaacaa actggtttct ctttttggtg tttcctaaag tagcaagtat atctttgttt
     5161 tttatgaggt taagagatag gatttgttct ttctttttta ttttttcttt tgctgttgtt
     5221 atgggtttat aatctgcaac agcagcactt aaaacagcaa tatttgaaag tttaaaatat
     5281 tgcaaggtag ccttatacat ttcatctgca cttgtaactg aaactacttt tataccttta
     5341 tattttgtgg ttacttgcac attacctgca actataatta cttctgcacc ttgtaaataa
     5401 aaagcttctg caatggcaaa gcccatttta cctgttgaat ggttagatat aaatctaact
     5461 gggtcaatag cttctatggt acctcctgca gtaattaata ttttttttcc tacaaactcg
     5521 gttgttctaa aaaaattttc aattaaaaaa gtaaaaattt cttcaggctc agccattctt
     5581 ccttctccta ttaagccact tgccaattct ccattattta catctataaa ttgtacagag
     5641 tcttttttta atgttgccat atttcgttta gtagcagcat gtttccacat atcttcatcc
     5701 attgctggtg caacaactac agggcatgta gcagataaat aaattgccat taaaacatta
     5761 tcgcaaatac catttgccat tttagctaaa gtattgcaac ttaaaggggc aattaaaaat
     5821 acatcagccc atcgtccaag cattacatga ttttcccaag aatcgttagt acttagttct
     5881 gagattgttt tatttttaga taaggtagat agcaccaatg gcgaaacaaa atcttttgct
     5941 gagggagtaa tcacaatttt aacatcagcc cctgctttta ccaacattct aactaacaaa
     6001 attgttttgt aagctgcaat gctgccagta atgccaataa gtattttttt atcttgtaac
     6061 atgattatgg attgctgcaa aataacaaaa aaacgacagt attactactg tcgttaatat
     6121 aaatatataa cttatcaatt atctaaataa atcttcgtcg ttgtttctat aataaatttt
     6181 gttttctaaa aactcttgtg ttgctaaaat tgcagcatta ggcatttttt cgtaagctct
     6241 ggatatttct atttgttctt tattttcatg tacttcttct aaattatcgg tatggcttgc
     6301 aaactcttct aacttatttt taagttcttc ttttaaagaa acatttattt gatttgctct
     6361 ttttccaata atagaaatag attcatataa atttccagta acagctttaa tatctgctaa
     6421 attttttgtt tcaattacac ttacagtatt ggcactaagc tgccttctta acttgctcat
     6481 tttttatatt tttaataaag ttagtggata aatttttata cttctcggct tctgcaaaat
     6541 atttactatc ttgaaatcta tctgtgaagt ctttacattc tttaattacc atttcaaatc
     6601 tttcttcctg tttggcaggt atgctcattt cggcatattt atagtaagat tttattacaa
     6661 gtaatttata ttcatcggct tttttagaat ctggaaaatt ttctgaaaca tttgaaaaac
     6721 aaattgcagc tgctttgtaa aaaccaagat tataatataa ctcggcactt ttatatcttt
     6781 ttaattcaag tttttctcta cacaaatcaa tgatgttaga agcatcttta actctagcag
     6841 aaccgggatg tttattaata aatgcttgca ttaaaccaat tgctttattg gtgtttgttt
     6901 gatctaaatc tacttttggt gattgtttat aataacaaaa tgccctcata tactcacact
     6961 cttctgcctt gttgctatta ggaaaagttt ctacaaaatt tttaaataaa ttttcggcat
     7021 ttaaataatc cttatcataa taataacagt aagcaaactt ataataagtg tcttcaaact
     7081 ctgcagttcc ttttacataa ggaaaaactt cttcaaacaa tatttgtgca aacctatatt
     7141 tcttttttgc ataatactgt tctgccattt ttaatttgta ttggtaatct ttgctctttt
     7201 gaatttttga aaatttatta gagcatgcac ccaatacaat tgtaaataat aaaaataaga
     7261 gtactctttt catttgaagt gtgcaaaatt acggatttgg cggcaattta tactgcaatt
     7321 atttataact ataattaaac tagttaatta ttacctaaaa cagggtttaa aagttttaat
     7381 ttttgttcaa gtatttagca aaaaagcaat cctttctgcg ttagtcaaaa agaattgctc
     7441 ctatattaca actttataac aaaatgattg ttagagagta gctaaaacat tattcatatt
     7501 tcttacagca tctgcacttt tattaaatgc atcttgctca gagctactta gttta
//
LOCUS       DAOQPK010000091         7439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_5555_length_7439_cov_4.672400, whole genome shotgun sequence.
ACCESSION   DAOQPK010000091 DAOQPK010000000
VERSION     DAOQPK010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7439
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5555_length_7439_cov_4.672400"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="PLU36_05720"
     CDS             <1..945
                     /locus_tag="PLU36_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46283.1"
                     /translation="VLGVLKELYPDGNFTQKILKGNTDSTSKIKTDEATKTYETIYNL
                     FIEGKFKEAIEAKTNADKIYSKNYWTPQLLFIEAIYYVKQKDDSTATVKLKELIKLYP
                     KTTLAAKAATMIDVLKRRKEIEDYLTKLEVERNEDPVTRNVDTEDDATVVGVKKEKKT
                     NEDTKEAPDKSTTKTEGTKKSTEEKKGVYNFIATDQQYIMIVFNKVDNLFIKESKDAF
                     AQFNRQKFYSKKINIIQHQLDEENTILLFGIFENTGLALEYYNVLKPQATTRIISFIP
                     PNKYKYSIISQANFNILKDSKNLTEYYNFVQQILPDKF"
     gene            964..3375
                     /locus_tag="PLU36_05725"
     CDS             964..3375
                     /locus_tag="PLU36_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HMZ46284.1"
                     /translation="MKKSVKIVWQITFGGIVAFVLFILLCSWGAFGKMPDLEDIQNPS
                     ASLSSQVYAQDGSIMGKYYLQDRINIQFKNIAQPVLDAVIATEDERFYTHSGIDLRAL
                     GRAFAGLGRDGGASTITMQTAKNLFTDNWATKNFIVRSIQKFKEAIIAVKLEKNFTKE
                     EIITLYLNTVAFSDNVYGIRNAAKTFFQKEPDSLNVKESAVLIGMLKGATLYNPRRNY
                     KTSFERRNVVMNQMVKNNKLSKASFDTLKLQPIDLNYRKLDENAGIGPYFRMILGEEL
                     KKWCKEHKKNNGKNYDLYRDGLKIYTTINPKMQVYAEQAVDKHISEMQKYLSSQSNIK
                     SGAVWKGRENVLDAAMKQSDRWRNMKSEGADEETIKKSFYEKTKMKVFSWNKNREADT
                     VMTPFDSIKYSRQMLQAGFMVMDPITGEVKAWVGGIDFKNYKYDHVNFNTKRQVGSTI
                     KPLLYGLAIEEAGLTPTSMVQDVQQSFGSYGLVPATSKTCTGATMPMAQALAGSRNCA
                     SAYIIKQLDNSGNNGARRFVDFLKRCDFKTKLDAYPSMALGACEISLYEMLQGYTMFP
                     GRGFNVQPMFITRIEDRNGNVLETFTPKRKEVISDVTAYSVIKMMEGVMEFGTGRRIW
                     SYDVKGKIAGKTGTTNDNSDAWFIGYTPQILAGTWVGCDDRFIRFNSTAIGQGASAAL
                     PIWAYFYEKASRDPACGLDVTSTFAKPEALTNDIIIDGISGFTPVPGGETEDGGAGTS
                     SEYGEESSEYNDDSTTPTQTNETQNSDNKNNQDNKNKTEKKQPPATLPNKDQKPKAVI
                     PKKNT"
     gene            complement(3372..4310)
                     /locus_tag="PLU36_05730"
     CDS             complement(3372..4310)
                     /locus_tag="PLU36_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HMZ46285.1"
                     /translation="MIQYFKNIEGKTVEISKPENDIWVNLVPPFKEEDFTDLSNNLDI
                     PIEFLRDSLDIDERSRYELEDNVKFIVIKTPAENNSFNDSDAFYITIPICIILTHNHI
                     VTVNSFDNGAIKKFLNTFQNRQTDKRNMMVLKIFEKIVQDYLGYLKQINNRRNQYEQS
                     LYDSNSNEDLLNLMRIQKSLTYFVTALRSNEMLMMKLERTNFLGLNEDDRELLRDLIV
                     DTSQALEMANIYANILSSTMDAFASIISNNLNNVMKRLTSITIILSLPILVTSIYGMN
                     VEIPYQHSSHAFYIPVILSLIISVVIGWYFLKKKWF"
     gene            complement(4406..5179)
                     /gene="dapF"
                     /locus_tag="PLU36_05735"
     CDS             complement(4406..5179)
                     /gene="dapF"
                     /locus_tag="PLU36_05735"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HMZ46286.1"
                     /translation="MTIFFSKYQGTGNDFIIIDNRENKINLSYQQINFLCNRKFGIGA
                     DGLMLLNSHSLYDFEMIYYNADGHESSMCGNGGRCITQFAHDIGLIKDKYLFTAIDGL
                     HEAVVKDIVYLKIQDVKGVENEHNEVFILNTGSPHYIKILPSITDLDVYTEGKKIRYN
                     SRFEKEGINVNFVQRINDNTIKVRTYERGVEDETLSCGTGVTAAALVNATTVGFNEVN
                     IQTIGGNLLVQFSKESDQVFHNIWLCGPANFVFSGSISI"
     gene            5573..6418
                     /locus_tag="PLU36_05740"
     CDS             5573..6418
                     /locus_tag="PLU36_05740"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HMZ46287.1"
                     /translation="MPKEIAFLINGQSKETLQEQFIEERNQREAIIEKNTTDANTQQL
                     KPKIKTIVYFPGSFAVFFLILTIGICSKAIGQWMSFERTKQQIAHQKVNAELSYLKSQ
                     VNPHFFFNTLNNIYSLAVVQSDKTAASVLKLSSIMRYMLTEVQEEKTLLEKEIDHIKN
                     YIDLQMVRLTNKVSVSFTVNGTIDGIKVAPLLLIPFIENAFKYGVSTVEQSAIEIEIN
                     TTEKCLQFISSNTIVQTQNILQSSTGIGISNVKRRLELLYKDNYTLNITNNTFNFKVF
                     LEINI"
     gene            6421..7116
                     /locus_tag="PLU36_05745"
     CDS             6421..7116
                     /locus_tag="PLU36_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ46288.1"
                     /translation="MISAIALDDEPLALQIIQQYCNKINFITLEKTFTNPNEAYLYLK
                     NNKINLLFLDIQMPDINGLQFFKSLPIKPSVIFTTAFKDYALEGFNVNAIDYLLKPFE
                     FERFLIAANKASTAIEGNNITTVQNFINVKVNYEIQKINLAEIELIEALDDYIKIYIK
                     PYPVLTLMTLKNMLEKLPADEFVRIHRSYIIPLNKVEKFNKHKVFVAGKEIPIGSSFS
                     SVYDILTQHNLQE"
     gene            7191..>7439
                     /locus_tag="PLU36_05750"
     CDS             7191..>7439
                     /locus_tag="PLU36_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46289.1"
                     /translation="MLNKHCTTVLFLLLFNYFASTAQNNFAIAINYTPVLNSNNFSTV
                     FGGVNGNQIKLDKQGLIREMEFIAYPKTVFNIVKTIPQK"
BASE COUNT         2872 a         1065 c         1100 g         2402 t
ORIGIN      
        1 gttttaggag tattaaaaga attataccct gatgggaatt ttacacaaaa aattttaaaa
       61 ggaaatacgg actctacctc aaaaattaaa acagatgaag caactaaaac atacgaaaca
      121 atttataatt tgtttataga aggcaagttt aaagaagcca tagaagctaa aacaaatgcc
      181 gataaaattt acagtaaaaa ttactggacc ccacaattat tatttattga agcaatttat
      241 tatgtaaaac aaaaagacga ttcaacagca acagtaaaac ttaaagagtt aattaaacta
      301 tatcctaaaa caacattggc tgcaaaagct gcaaccatga ttgatgtttt aaaaagaaga
      361 aaagaaatag aagattattt aacaaaatta gaagtagaac gaaacgaaga ccctgtaaca
      421 agaaatgtag atacagagga tgatgcaact gttgtaggag ttaaaaaaga gaaaaaaaca
      481 aacgaagaca ccaaagaagc tccagataaa agtaccacaa aaactgaagg aacgaaaaag
      541 tcaacagaag agaaaaaagg tgtttataac tttattgcaa cagaccagca atacattatg
      601 attgtattta ataaagttga taatttattc attaaagaat cgaaagatgc atttgcacaa
      661 ttcaacaggc aaaaattcta ttccaaaaaa ataaatatta tacaacacca actagacgaa
      721 gaaaatacaa ttttactttt tggaatattt gaaaatacag gtttggcttt agagtattac
      781 aacgttttaa aaccacaagc cactaccaga ataatttcat ttattccacc taacaaatac
      841 aagtatagta ttataagcca agctaatttt aatattttaa aagatagtaa aaacttgaca
      901 gagtattata attttgtaca gcaaatttta ccagataagt tttaaaataa gcactaatta
      961 aacatgaaga aatcggttaa aatagtttgg caaattacat ttggtggcat tgttgcattt
     1021 gttctattta tattattatg cagttggggt gcttttggta aaatgccaga tttagaagac
     1081 attcaaaacc catcagcatc attaagtagc caggtatatg cacaagatgg ttccataatg
     1141 ggcaaatatt atttgcaaga tcgtattaat atacaattta aaaatatagc ccagcctgtt
     1201 ttagatgcag ttatagcaac cgaagatgaa cgcttttata cacatagtgg aattgattta
     1261 agagcattag gaagagcctt tgcaggctta ggaagagatg gtggtgcaag caccataacc
     1321 atgcaaacag caaaaaacct atttacagat aattgggcta ctaagaattt tattgtaaga
     1381 agtattcaaa aatttaaaga agcaattatt gctgttaagc tagaaaaaaa ttttacaaaa
     1441 gaagaaatta ttacactcta tctaaatact gtagcattta gcgataatgt ttatggaata
     1501 agaaatgcag caaaaacatt ttttcaaaaa gaaccagata gtttaaatgt taaagaatct
     1561 gcagttttaa ttggaatgct taaaggagca acactttata atccaagacg taattacaaa
     1621 acatcttttg aacgtagaaa tgttgtaatg aaccaaatgg tgaaaaataa taaattaagt
     1681 aaagcaagtt ttgatacatt aaagctacaa cccatagatc ttaattatag aaaattagat
     1741 gagaatgctg gcataggtcc atactttaga atgattttag gagaggagtt gaaaaaatgg
     1801 tgtaaagagc ataaaaaaaa taacggaaaa aattacgatt tgtatagaga tggcttaaaa
     1861 atttatacaa ccattaatcc caaaatgcag gtatatgccg aacaagcggt agataagcat
     1921 attagtgaaa tgcaaaagta cttaagttct cagtctaaca taaaatctgg tgcagtatgg
     1981 aaaggaagag aaaatgtgtt agatgcagca atgaaacaaa gtgatagatg gagaaatatg
     2041 aaatcagaag gtgccgatga ggagacaatt aaaaaaagct tttatgaaaa aactaaaatg
     2101 aaagtttttt catggaataa aaatagagaa gcagatacag taatgacacc atttgactct
     2161 ataaaatata gtaggcaaat gttacaagca ggttttatgg taatggatcc aattactgga
     2221 gaagtaaaag cctgggttgg tggaatagat tttaaaaact ataaatacga ccacgtaaac
     2281 tttaatacta aacgccaggt aggctctacc attaaaccat tactttatgg tttggcaata
     2341 gaagaggcag gccttacacc aacatcaatg gtacaagatg tacaacaaag ttttggttct
     2401 tatggcttag tacctgcaac aagtaaaaca tgcacaggtg ctacaatgcc tatggcacaa
     2461 gcattagctg gttcaagaaa ctgtgcatct gcttatatta taaaacagtt agataattct
     2521 ggcaataatg gagcaagaag gtttgttgat tttttaaaac gttgcgattt taaaactaaa
     2581 ttagatgctt accctagtat ggctttaggt gcttgtgaaa tttcgttata tgaaatgtta
     2641 caaggctata ccatgtttcc tggaagaggc tttaatgtac aacccatgtt tattacacgt
     2701 attgaagata gaaacggaaa tgtattagaa acttttactc caaagcgtaa agaagttatt
     2761 agtgatgtta cagcatactc agtaataaaa atgatggaag gagtaatgga atttggaaca
     2821 ggtagaagaa tttggagtta tgatgtaaaa ggaaaaattg caggtaaaac aggaaccacc
     2881 aatgacaata gtgatgcttg gtttatagga tatacacctc aaatattagc aggtacttgg
     2941 gttggttgcg atgatagatt tattcgattt aattcaacag caatagggca aggtgcttct
     3001 gctgctttac caatttgggc atatttttat gaaaaagcat ctagagaccc tgcatgtggt
     3061 ttagatgtaa ccagcacttt tgctaagcca gaggcattaa ctaatgatat tattattgat
     3121 ggaataagtg gctttacccc tgtaccaggg ggtgaaacag aagatggcgg agcaggcact
     3181 agctctgaat atggggagga aagtagtgaa tataatgatg actctaccac acctactcaa
     3241 acaaatgaaa ctcaaaattc agacaataaa aataatcaag acaacaaaaa taaaacagaa
     3301 aaaaaacaac cacctgctac tttacccaat aaagatcaaa aacccaaagc tgttattcca
     3361 aagaaaaata cttaaaacca tttcttcttt aaaaaatacc aaccaataac taccgatatt
     3421 attaaagaga gaattacagg tatataaaat gcatgtgaag agtgttggta cggaatttca
     3481 acattcattc cataaatact ggttactaaa attggtaacg ataaaataat agtaatagaa
     3541 gttaaacgtt tcattacatt atttaaatta ttgcttataa tacttgcaaa tgcatccatt
     3601 gtactactta aaatatttgc ataaatattt gccatctcta aggcctggct ggtatctaca
     3661 atcaagtctc taagcaattc tctatcatct tcattcagcc ctaaaaaatt tgttctttct
     3721 aatttcatca ttagcatttc attgctacgc aaagcagtaa caaaataagt aaggcttttt
     3781 tgaatacgca ttaaatttaa taaatcttcg ttactgttac tatcgtataa actttgttcg
     3841 tactggtttc tgcgattatt aatttgtttt aaatagccta aataatcttg tacaatcttt
     3901 tcaaaaattt ttaataccat catatttctt ttatctgttt gcctattttg aaaagtgttt
     3961 aaaaactttt taattgctcc attgtcaaaa gagttaacag taacaatatg gttatgggtt
     4021 aaaatgatgc aaataggtat tgtaatataa aaggcatcgc tatcattaaa agaattattt
     4081 tcggctggag ttttaattac tataaatttt acattgtctt caagttcata tctagatctt
     4141 tcgtcaatat ctaaagaatc tctcaaaaac tcaataggaa tatctaaatt attacttaaa
     4201 tcggtaaagt cttcttcttt aaaaggtggc actaggttta cccaaatatc attttctggc
     4261 ttacttattt ctacggtttt accctctatg tttttaaaat actgaatcat aaataaccca
     4321 tttgtttttg ttagaaataa gtattaaggc aacgaaatta cagtatgttt cctaaacaaa
     4381 aatttcaatt tttaagtaga agaagttata tagaaatgga acctgaaaaa acaaaattgg
     4441 caggaccaca tagccaaata ttatgaaaca cctgatcact ttcttttgaa aattgaacta
     4501 ataaattacc tccaatagtt tgtatattaa cttcattaaa ccctactgta gttgcattaa
     4561 ctaatgcagc agcagttaca cctgtaccac aacttaatgt ttcatcttct acacctcttt
     4621 cataagttct tactttaatg gtgttatcat taatacgttg aacaaagttt acatttatcc
     4681 cttctttttc aaacctagag ttgtatctta tttttttacc ctcagtatat acatctaaat
     4741 ctgtaatact tggcaaaatt tttatgtaat gtggagatcc tgtatttaaa ataaaaactt
     4801 cgttatgttc attttctaca cctttaacat cttgaatttt taagtacaca atgtctttaa
     4861 caactgcttc gtgtaaacca tctattgcag taaataaata cttatctttt attaaaccaa
     4921 tatcatgtgc aaattgtgta atacatcttc ctccattgcc acacatagag ctttcgtgcc
     4981 catctgcatt atagtaaatc atttcaaagt cgtataggct atggctattt agtaacatta
     5041 atccatctgc accaatgcca aactttctat tacataaaaa atttatttgt tggtaagata
     5101 aatttatttt gttctcacgg ttgtcaataa ttataaaatc attgcctgtg ccttggtatt
     5161 tagaaaaaaa aatggtcatt aataaaagaa atgaatggtt attaaaggtt tttcatacaa
     5221 aagtaaataa ctatttacta taaatttact actatataaa tctaaaaatt aagctacttt
     5281 agctatctca atttttaata aattgtttaa acaaaaaaaa atactaaata tcagtattca
     5341 tattgcagta tggatttgct tttttctatt accttatatt ttttctcctc aaccaaaaga
     5401 tatttcagtt caagtttcaa aatatttagt ggtactatac attgttataa atattttttt
     5461 acttctcttt tattacttta atacacttgt attaatacca aaattattat tttaaaaaag
     5521 atggtttata tactctgtaa ttatttccat tttttttatt gcctatttgt atgtgccaaa
     5581 agaaattgct tttttaataa atggtcaatc taaggaaaca ttgcaagagc aatttattga
     5641 agaaagaaat caacgtgaag caattattga aaaaaatact actgatgcaa atactcaaca
     5701 acttaaacct aaaattaaaa ccattgttta ttttcctggg tcgtttgcag ttttttttct
     5761 aatacttacc attggtatat gtagcaaagc tataggacag tggatgagtt ttgagagaac
     5821 taaacaacaa attgcacatc aaaaagtaaa tgcagagttg tcctatctta aatctcaggt
     5881 aaaccctcac ttttttttca atactctaaa taatatttac tctttagctg ttgtgcaaag
     5941 cgataaaaca gcagcatccg ttttaaagct ttcttctata atgaggtata tgctaaccga
     6001 agtacaagaa gaaaaaactt tacttgagaa ggaaatagac catattaaaa attatataga
     6061 tttacaaatg gtgaggctta caaataaagt gtctgtaagt tttactgtaa atggaacaat
     6121 tgatggcata aaagttgcac cactattatt aattcctttt attgaaaatg cttttaaata
     6181 tggtgtaagc acagttgaac aatctgcaat tgaaattgaa ataaatacca ccgaaaaatg
     6241 tttacaattt atttcttcta ataccattgt gcaaacacaa aatattttac agtcatctac
     6301 aggaattgga ataagtaatg taaaaagaag attagagttg ttgtataaag acaattacac
     6361 acttaacatt actaataata cttttaattt taaagttttt ctagaaataa atatataaaa
     6421 atgattagtg caatagcttt agatgatgag cctttggctt tacaaattat tcaacagtat
     6481 tgtaataaaa ttaattttat tactctcgaa aaaactttta ctaaccccaa tgaagcatat
     6541 ttatatttaa aaaataataa aattaatttg ttgtttttag atattcaaat gcctgatata
     6601 aatggcttac aattttttaa aagtttacca attaagcctt ctgtaatttt caccactgct
     6661 tttaaagatt acgctttaga agggtttaac gttaatgcaa ttgattactt attaaagcct
     6721 tttgagtttg aaagattttt aatagctgcc aataaagcaa gcacagcaat tgaaggaaat
     6781 aatataacaa cagtgcaaaa ctttattaat gtaaaagtaa actacgagat acaaaaaata
     6841 aatttagcag aaatagaatt aattgaggca ttagatgatt atataaaaat ttatataaaa
     6901 ccctatcctg tgttaacttt aatgactctt aaaaacatgc ttgaaaaatt accagcagat
     6961 gaatttgtta gaatacatcg ttcttatata ataccactta ataaagtaga aaagtttaat
     7021 aaacataaag tatttgtagc aggaaaagaa ataccaattg gcagcagttt ttcttctgtt
     7081 tacgatatat taacacaaca caatttacag gagtagtatt tgtttatatt tttttacatt
     7141 tttatttcaa tttttactct caaatttgct ttaaacgtaa acataaatgt atgttgaaca
     7201 aacactgcac tacagtatta tttttattac tatttaatta tttcgcctcc actgctcaaa
     7261 ataattttgc tattgcaata aactatacac ctgtattaaa ctccaacaat ttttcaacag
     7321 tgtttggagg cgtaaatggc aaccaaataa aattagacaa gcaaggttta ataagagaaa
     7381 tggaatttat agcatatcct aaaactgtgt ttaatattgt aaaaacaata ccacaaaag
//
LOCUS       DAOQPK010000092         7374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_5646_length_7374_cov_4.596393, whole genome shotgun sequence.
ACCESSION   DAOQPK010000092 DAOQPK010000000
VERSION     DAOQPK010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7374
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5646_length_7374_cov_4.596393"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PLU36_05755"
     CDS             <1..232
                     /locus_tag="PLU36_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HMZ46290.1"
                     /translation="GRNTSVYAPAHICIAFTNQLVYDIGEGLTLLQEKYINQLPSLIT
                     LASGPSRTADIEKTLVTGIHGPKEVFVFLVES"
     gene            381..761
                     /locus_tag="PLU36_05760"
     CDS             381..761
                     /locus_tag="PLU36_05760"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HMZ46291.1"
                     /translation="MKRLLLLLSVGLFSFITLNQSDNNDIVKALKSGSAEQIATYFDS
                     FIDLKLPEKDEIKNMGKNQAGIALKTFYNDNNIKGFDLTSQREMSGTMYIAGKLKNNN
                     KGYNITVMAKKTNDKFGIISIRIN"
     gene            847..1350
                     /locus_tag="PLU36_05765"
     CDS             847..1350
                     /locus_tag="PLU36_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ46292.1"
                     /translation="MDNIILRPWKKEDAQALAAIANNKNIWNNVLDNFPSPYTVTDAL
                     QWINRESAIIPVSKFAIECNHKIVGGIGLLTYDDIYKCSIELGYFIGEPFWGLGIATK
                     AIEKIVQHIKDDRPGVKRIFARVFENNKASMKALQKAGFYLEGIQKNAAVKNSELVNI
                     YVLVKLV"
     gene            complement(1523..1879)
                     /locus_tag="PLU36_05770"
     CDS             complement(1523..1879)
                     /locus_tag="PLU36_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMZ46293.1"
                     /translation="MNIKPIKTKKDYEQAMLRLEKLFDAKKGTSKGDELEVISMLVEK
                     YEDEHFPIDLPDPVEAIKFRMEQLGMTQTDLANLVGQKSRASEILNRKRKLSLDMIRQ
                     LHQQWRIPTEVLVQAY"
     gene            complement(1881..2201)
                     /locus_tag="PLU36_05775"
     CDS             complement(1881..2201)
                     /locus_tag="PLU36_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HMZ46294.1"
                     /translation="MLTFDKTRRLRVIAKKVLRDFWEKHCDCEQQLKAWFLETSKAEW
                     INPNEIKVEYPSASIIGNDRIVFNIKGNKYRLIVKINFDYQMVWIRFIGTHSEYDKVN
                     AKTI"
     gene            complement(2273..4261)
                     /locus_tag="PLU36_05780"
     CDS             complement(2273..4261)
                     /locus_tag="PLU36_05780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ46295.1"
                     /translation="MFVISCNSNSKNKLNSERLPAIDTIIDKAGKISFLGGEPALKFI
                     DSATYNKKFSTKEKIRLFGYLSYLYSKYLHNIEKSALYSDSMFYLLNNKNIDDYSYEY
                     SYSYFTRGDVLFDQRKFGEAYSYYYKGKQVGVAFYDSATLGEYSYRLGHVLYLQGKFN
                     EAKLSFMQALELWKTATLTFGKYYATQEIISNIGLCYYKANQPDSSLFYFKKALNYID
                     NAEQNYINRKELHNAAKGVIFGNIGKVYEKTKQYDSALTYYKKGVIINLADGYEIEDA
                     LINNIHVAQVYGIQHKYDSMKNTLLQTEKWLKKSSEYKLANLMWHKQMWQYYDKTNQT
                     QQAYTYLTKYTELFTQHSKETISINSINIDQQVEFLESKKAIEVMQKESKIKEIYLYG
                     SILLVVLMSGFIVVTLIFWNKSKQNIKKLQQLNNTIQHQNFILETQSREKEKILKLVA
                     HDLRTPIASIAALTDMLEEEKEKEPQKELIGIMKMACDSSLKLISEILESSSSTNKEI
                     EKEMVSASDCLSDCISQLQINAAEKKQKFVIKPLPYAVSLYVNKDKMKRVMYNLITNA
                     IKFSHEGGEIHLKMEQHNDKSILISIQDFGIGIPDHIKTNIFNLNDEAKRSGTKGEKS
                     FGLGLTICKDIVAAHNGKIWVESTENKGTTFFVELPLS"
     gene            complement(4539..6326)
                     /locus_tag="PLU36_05785"
     CDS             complement(4539..6326)
                     /locus_tag="PLU36_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMZ46296.1"
                     /translation="MSTQQLTTLHGGEWIIKESNALEVFTPEDFNEEQKMVMDMCHQF
                     LQADVLPNLEKIDKQEDNIVPKLLDMAGEQGLLSTTFPEQYGGLGKDFITGTIVNEGL
                     GAGHSFSVAIAAHTGIGSLPILYFGTEEQKQKYIPKLASGEFKGAYGLTEPNSGSDAL
                     SAKTTAKLSEDGKYYILNGQKCWITNGGFADVYTVFAKVDGTQFTAFIIERGFEGFTQ
                     GPEEHKMGIKGSSTVQLYFQDCKVPVENLLGEIGKGHIIAFNILNIGRLKLCAAALGG
                     SKSAATTSVQYANTREQFKLPIAKFGAIKHKLAEMAIKIWVTETALYRTAKWIDNKEH
                     ELLEAGKPFNEALLGAAEEYAIECAILKVFGSETLDFVVDEGVQIHGGNGFSDEYVIS
                     KAYRDSRINRIYEGTNEINRLLTVDMVLKRAMKGKLDLMTPAMNVQKELMSIPDFGTE
                     EDGAFSKELKYIANFKKAILMVAGAAVQKLMMQLDKEQEVLMNIADMAIEVFHSESAL
                     LRVIKLYNQHGAEATSLQTDMMRTYLYDAADKINKAGKDALNSFADGDELRMMHIGLK
                     RFTKVEPFNTKEARRRICNKMLEANAYCY"
     gene            complement(6365..6805)
                     /locus_tag="PLU36_05790"
     CDS             complement(6365..6805)
                     /locus_tag="PLU36_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HMZ46297.1"
                     /translation="MNNQFKRGELYSVLNAMAATAIARRLQKKFHQNQLEITIEQWSI
                     LYHLWKNDGLSQQDLCNKTFRDKPSITRLIDNLEKLKLVKRVSNKDDKRVNSVFLTDA
                     AKNLHDLTIQLANQTMDEALIGVNKTEIETVKNVLQKVYDNLTS"
     gene            complement(6924..>7374)
                     /locus_tag="PLU36_05795"
     CDS             complement(6924..>7374)
                     /locus_tag="PLU36_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ46298.1"
                     /translation="AITLVLGKGFWQVFIAVGLTMWVNVARLVRGQVMIAKELEYIEA
                     ARVLGFNHFRIIVKHILPNIIGPILVITAANFAGAIVIEAGLSFLGIGVQPPQPSWGL
                     MIKDHYNFIVTNNAILALIPGIAIMLLVLAFNILGNGLRDALNVRTT"
BASE COUNT         2373 a         1231 c          995 g         2775 t
ORIGIN      
        1 tggaaggaat acaagtgtat atgccccagc acatatttgt atagctttta ccaaccaatt
       61 agtttatgat attggtgaag ggcttacttt acttcaagaa aaatacatta accaacttcc
      121 ttccttaata acattagcct ctggtcctag cagaactgca gatattgaaa aaacattggt
      181 tactggcatt catggcccca aagaagtatt tgttttttta gtagaaagct aacatattca
      241 gtaaaattca aaactaagtt aaaaatatat ggataaatta ttatcttgcc tttaggcata
      301 ttgtaatcaa atggttaatt tggcatgttt ttagtgcagt agttaaacaa gtatttattt
      361 attttttaaa aagaatttca atgaaacgtt tgttgctatt actttcagtt gggttatttt
      421 cttttattac acttaaccaa tcagataata atgatattgt aaaagcactt aaatcgggta
      481 gtgcagaaca aattgctacc tattttgata gttttattga tttaaaacta cccgaaaaag
      541 acgaaataaa aaatatgggt aaaaaccaag ctggtattgc tttaaaaact ttttataacg
      601 ataataatat taaaggattc gacctaacct ctcaaagaga aatgagcggt actatgtaca
      661 ttgcaggaaa attaaaaaat aataacaagg gctataatat tacagtaatg gcaaaaaaaa
      721 caaatgataa atttggtatt atatctatta gaataaacta atttctctct cttagactac
      781 ttctttttta caaatattat cttttatatt tttgattaac tttagtgctt actcctaaca
      841 tcaatcatgg ataatattat tttaagacct tggaaaaaag aagatgctca agccctggct
      901 gctattgcca ataataaaaa tatttggaat aatgtattgg ataattttcc tagcccttat
      961 actgtaacag atgctttgca atggataaat agagaaagtg ctataatacc cgtttcaaaa
     1021 tttgctattg aatgtaacca taaaattgtt ggtggtattg gtttattaac ttacgatgat
     1081 atttataaat gcagtataga gttaggttat tttattggtg aaccattttg gggtttgggt
     1141 attgctacca aagctattga aaaaattgta cagcatatta aagatgatag gcctggagta
     1201 aaaagaattt ttgcaagagt ttttgaaaat aataaagcca gtatgaaagc cttacaaaaa
     1261 gcaggttttt atttagaagg tattcaaaaa aatgctgctg taaaaaacag tgaattagta
     1321 aatatttatg ttttggtaaa attggtgtaa gtaaattatt acattaagtt tgagaaagaa
     1381 caaagtgtaa actactatca ggtttacaaa aattactgta aaccgatggt agtacaaaca
     1441 ttgaaaaatt gtcaactaat tttaggacga tacacttcac ccaccatatt accaatgcaa
     1501 cgttggcaga agtacgttgt aattagtaag cttgcaccaa aacctctgta ggtatacgcc
     1561 actgttgatg caactgtcta atcatatcta acgagagttt tcgcttccga ttcaatattt
     1621 cacttgcacg acttttttgc ccaacaagat tagctaagtc tgtttgtgtc attccaagtt
     1681 gctccattct aaattttata gcttcaactg ggtctggcag gtctattggg aaatgttcat
     1741 cttcgtattt ttcaactaac atacttatta cttctaattc gtcccccttg gatgttcctt
     1801 tcttggcgtc aaatagtttc tcaagcctta gcattgcttg ttcgtagtcc ttctttgtct
     1861 tgatgggctt gatgttcata ttaaatagtt tttgcgttta ctttgtcata ttcggaatgt
     1921 gtcccaataa accgtatcca taccatttga taatcaaaat tgatttttac tattaagcga
     1981 tacttgttgc cttttatatt gaatacaatc ctgtcgttgc caataatgct tgcactcgga
     2041 tactcaactt ttatttcgtt gggatttatc cattcggctt tagaggtttc cagaaaccat
     2101 gcttttaatt gttgctcaca atcacagtgt ttctcccaaa aatctctaag tactttcttt
     2161 gcaattactc tcaaccttct tgttttgtca aaagtaagca aaagttacca aaatgggaaa
     2221 tgtaattttt atgaaaattt caatccgtac ttggtgtaag taaattatta cattaactta
     2281 aaggcaattc tacaaaaaat gttgtgcctt tattttcagt actttcaacc catattttac
     2341 cattatgtgc agccacaata tctttacaga tagttaaacc taaaccaaaa gatttttcgc
     2401 ctttagtacc acttcttttt gcttcatcat ttaaattaaa aatattagtt ttaatatgat
     2461 cgggtatgcc aataccaaaa tcttgaatag aaattaaaat agatttatca ttatgctgct
     2521 ccatttttaa atgtatttca cctccctcgt ggctaaattt aatagcattg gtaattaggt
     2581 tatacattac ccttttcatt ttatctttat taacgtacaa gctaactgcg taaggcaaag
     2641 gctttataac aaatttttgt ttcttttctg cagcattaat ttgtaattga gaaatacaat
     2701 ctgataaaca atcactggca cttaccattt ctttctcaat ttctttgttt gtgcttgaag
     2761 agctttctaa aatttcagaa attaacttta aagaactatc acatgccatt ttcataattc
     2821 caatcaactc tttttgaggt tctttttctt tttcctcttc taacatatct gttaatgcag
     2881 caatggatgc aattggtgtt cttaaatcgt gtgcaactaa ttttaaaatt ttttcctttt
     2941 ctctgctttg tgtttctaaa ataaaatttt gatgttgaat agtattatta agctgctgta
     3001 gtttcttaat attttgcttc gacttgttcc aaaaaattaa agtaaccaca ataaagccac
     3061 tcattaaaac aactagtaaa atggatccat ataaataaat ttctttaatc ttcgattctt
     3121 tttgcattac ttctattgcc tttttagatt ctagaaattc tacttgctga tcaatattga
     3181 tggagttaat tgaaatggtt tctttagagt gctgagtaaa taattctgtg tattttgtta
     3241 agtaagtata ggcttgttgg gtttgatttg ttttatcata atattgccac atttgtttat
     3301 gccacattaa attggctaac ttatattctg aagatttttt tagccatttt tcagtttgca
     3361 gtaatgtgtt tttcatacta tcatatttat gctgtatgcc ataaacttgt gcaacatgaa
     3421 tattatttat taatgcatct tctatttcat aaccatctgc taaattaatt attactcctt
     3481 ttttataata cgttaatgcc gaatcatact gtttagtttt ttcgtacact ttaccaatat
     3541 taccaaaaat taccccttta gcagcattat gtaattcctt tctattaata taattttgct
     3601 ctgcattatc tatatagttt agtgcctttt taaagtaaaa tagtgaacta tctggttggt
     3661 ttgctttata ataacacaaa ccaatattgc taataatttc ttgcgtagca tagtattttc
     3721 caaatgttaa tgtggctgtt ttccataatt ccaaagcctg cataaaactt agcttagctt
     3781 cattaaattt accttgcaaa tacaatacat gccctaacct atagctatac tctcctaagg
     3841 ttgcagagtc ataaaatgca acacctactt gtttgccttt atagtagtaa gagtatgctt
     3901 ctccaaactt tctttgatca aataaaacat ctcctcgtgt aaaataagaa taagaatatt
     3961 cataggaata atcatctatg tttttattgt ttagaagata aaacatagaa tcggagtaca
     4021 aagcagactt ttcaatatta tgtaaatatt tagagtataa ataagaaaga tagccaaaca
     4081 gtcttatctt ttcttttgta gaaaattttt tgttgtaagt agcagaatca ataaatttca
     4141 aggcaggttc gccacctaaa aaagaaattt ttccagcttt atcaataata gtatctattg
     4201 caggtaaccg ttctgaatta agtttatttt tagaattaga attacaagaa ataacaaaca
     4261 atatcagaat tggatacaag aaactaatag aaatgatatg actattcttt ttaaagtgca
     4321 tttataggtt ttaatgaatt aaaatacatt aattgcttgt ttaaaatgca accaattatc
     4381 tttaattgca aatagcttaa tatttattaa atagttttta gcttacaatc taataatatt
     4441 ttacttttta aaaaacttat ttcaaaatta agataattaa atttaatatg ttactcaaaa
     4501 gggtaaattt aaagtgataa aatttaccct tttgaggcct aataacaata ggcattggct
     4561 tctaacattt tattgcaaat tcttcttctt gcttctttgg tgttaaaagg ttcaactttt
     4621 gtaaaccttt ttaatccaat atgcatcatt cttaattcat caccatctgc aaaactgttt
     4681 agagcatctt ttcctgcttt attaatttta tcggcagcat catataaata agttctcatc
     4741 atatcagtct gtagagatgt tgcctctgca ccatgttgat tatatagctt tataaccctt
     4801 aataaggcac tttctgaatg aaaaacttca atagccatat cagcaatatt catcaatact
     4861 tcttgttctt tatctaactg catcattaac ttctgaactg ctgcaccagc aaccattaaa
     4921 attgcttttt tgaagttggc aatatatttt aattctttgc taaaagctcc atcctcctca
     4981 gtgccaaaat ctggaatgct cattaattct ttttgtacgt tcatagcagg tgtcattaaa
     5041 tctaatttgc ctttcattgc acgttttaat accatatcaa cggtaagtaa tctatttatt
     5101 tcattagtgc cttcataaat tctatttatt ctgctatctc tataagcctt acttattaca
     5161 tactcatcgc taaagccatt gcctccatga atttgcactc cttcatctac tacaaaatct
     5221 aatgtttcac taccaaatac ttttaatatt gcacattcaa tagcatattc ttctgcagct
     5281 cctaataatg cttcattaaa aggttttccg gcttctaata attcatgctc tttattgtct
     5341 atccattttg cagttctgta taatgcagtt tctgtaaccc aaatttttat tgccatttct
     5401 gccaacttat gttttattgc accaaatttt gcaatgggca atttaaattg ctctctggta
     5461 ttagcatatt gaacagaggt tgttgcagca cttttactac cacctaatgc tgcagcacat
     5521 aattttaacc ttccaatatt taaaatatta aacgcaataa tgtggccttt gccaatttct
     5581 cctaataaat tttctacggg tactttacaa tcttgaaaat ataattgaac agttgatgat
     5641 cctttaatac ccattttgtg ttcttctggt ccttgcgtaa atccttcaaa acctctttca
     5701 ataataaatg ctgtaaactg tgttccatct acttttgcaa atactgtgta aacatctgca
     5761 aagcccccat tagtaatcca acatttttgc ccatttaaaa tataatattt tccatcttca
     5821 cttagtttgg ctgtagtttt tgcactcagt gcatcgctgc cactattggg ttctgtaagt
     5881 ccataagctc ctttaaattc gccacttgcc aatttaggaa tatatttttg tttttgttct
     5941 tctgtaccaa aatataaaat tggtaagcta ccaatacctg tatgtgcagc aatagcaaca
     6001 ctaaaactgt gtcctgcacc taaaccttca ttaacaatgg tgcctgtaat aaaatctttt
     6061 ccaagccctc catattgctc aggaaatgta gtagatagta atccttgctc acctgccata
     6121 tctaataact ttggaacaat attatcttct tgtttatcaa ttttttctag attaggtagt
     6181 acatcggcct gtaaaaattg atggcacata tccatcacca ttttttgttc ttcattaaaa
     6241 tcttctggtg taaaaacttc taaagcatta ctttctttta ttatccattc tccgccatgt
     6301 aatgttgtta attgttgtgt gctcatagtt tgtagtatat atttttatta ttgggtgaat
     6361 tattttacga ggttaaatta tcgtacactt tttgtaatac attttttaca gtttcaattt
     6421 cagttttatt aacaccaatc aaggcttcat ccattgtttg attggcaagt tgtatggtta
     6481 aatcatgtaa gttttttgct gcatcggtta aaaaaacaga atttactctt ttatcatcct
     6541 tattactaac tcttttaact aattttagtt tctctaaatt gtctataagt cttgttatac
     6601 ttggtttatc tctaaaagtt ttattgcata aatcttgctg gcttaatcca tcatttttcc
     6661 ataaatggta tagaatgctc cattgttcaa tagttatttc taattggttt tgatgaaatt
     6721 ttttttgtaa ccttctggca atagcagttg cagccattgc atttaaaacg ctgtatagtt
     6781 cacctctttt aaattgatta ttcattataa tagttgttta tgcaactatt caaaggtaag
     6841 gagaatttta aattaacaaa tttttgttga agaaatatta aaagcagcgt ttaataaaag
     6901 tatggaacag ttaaagtttg ctattaagta gttcttacat tcaaagcatc tctaaggcca
     6961 ttgcctaaaa tattaaaggc taaaaccaat agcataattg caatacctgg aattagagcc
     7021 aaaattgcat tgttagttac aataaaatta taatgatctt ttatcattaa accccaacta
     7081 ggttgtggcg gttgtacacc aattcctaaa aaacttaagc ctgcttctat aacaattgca
     7141 ccagcaaaat ttgctgcagt aataaccaaa ataggtccaa ttatattagg taaaatatgt
     7201 ttaacaataa ttctaaaatg attaaagcct aacactcttg cagcttcaat atattctaat
     7261 tcttttgcaa tcattacctg accacgaact aatctagcta catttaccca catggttaaa
     7321 ccaactgcaa taaaaacttg ccaaaaacct ttgcccaata caagtgtaat ggca
//
LOCUS       DAOQPK010000093         7318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_5707_length_7318_cov_4.454082, whole genome shotgun sequence.
ACCESSION   DAOQPK010000093 DAOQPK010000000
VERSION     DAOQPK010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7318
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5707_length_7318_cov_4.454082"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PLU36_05800"
     CDS             complement(<1..264)
                     /locus_tag="PLU36_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46299.1"
                     /translation="MNVAIQKLQSNLFNNIEVEVLRTDEIHLIVSGNKWFKLKYFIQD
                     CIKKGKDTLATFGGTYSNHIVATAYACKSAGLKSIGIIRGEATK"
     gene            complement(315..974)
                     /locus_tag="PLU36_05805"
     CDS             complement(315..974)
                     /locus_tag="PLU36_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ46300.1"
                     /translation="MKTEKIIRLALADDHKIFRDGLRNTLKDKEYIKILWEAEDGKDM
                     THKLKMKEPDVLLMDIRMPEMDGIEAIKLIRHTYENLKIIVLSMYDDQEIITKMMEMG
                     ANSYLTKTTDPEEIYQAILTCMNEDFYFNDLVNTAVLLKLQHKKSVRRFYPTPAQFNE
                     KELRIIKLISEDKTTEEISKEVFLSPRTIETIRQAMKQKAGVKTIAGLIMFAMRNKLL
                     E"
     gene            1355..1765
                     /locus_tag="PLU36_05810"
     CDS             1355..1765
                     /locus_tag="PLU36_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46301.1"
                     /translation="MAKELIVVKGRVIEQNRSFLPNVGEDIGYELGAKMIKRWHTENP
                     DDVLASFTGRELIEKILAQPGCTGIRMFNAINDLGIKQLVLVGADKNGNNILEYTTIG
                     NNGEVIKHKGIVGDRSAICPPSCGQGDMDLDWSS"
     gene            1825..2520
                     /locus_tag="PLU36_05815"
     CDS             1825..2520
                     /locus_tag="PLU36_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46302.1"
                     /translation="MNYLFHISVFSPLIPILFFVLFKFNSKQRAKWVIFLLLVISLIT
                     DIWCYNLARNNIKTTWIINIFSVIEFAFLSYFFTLIISLKKQQIFIWLCYAGFLLYWL
                     VQNIFVESIEIFDYQSQAIEFLLLLICCLLYLFQRVKNVRGEILYNTYQFWIVAALLM
                     YCAGTFFGFLSSVSPEERDFDTIVYEYLSRGGNILKHILITIAFCINPANQKNEHPNK
                     NSIYYIKDNLGKD"
     gene            2527..3372
                     /locus_tag="PLU36_05820"
     CDS             2527..3372
                     /locus_tag="PLU36_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HMZ46303.1"
                     /translation="MQLLINFTQILLTTQITPVLFLGTIGMILLATSIILFAIFHQRR
                     VMRFQQRIQQMEVEHQKMLLKASIKLQEEERTRLAADLHDDAGPLLATAKLYLNDNFV
                     NLDKTAQMHSLFNAKQIIDDTIQLIRNISHSLMPPTLKNFGLSSAVNDFFQKISNSGS
                     LNASSKFHDYKTRLKSDKELILFRVIQELVNNVLKHSKASFIHLTQHDVEEKVILRLH
                     HDGFGLTQEEFLRYTNEKEGLGLKNIQSRLRVIHGDILYEKDSSQSLYKVTIEIPREE
                     GEEIK"
     gene            complement(3520..4086)
                     /locus_tag="PLU36_05825"
     CDS             complement(3520..4086)
                     /locus_tag="PLU36_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ46304.1"
                     /translation="MQVQFINSENTQIQKCIEGCRKSQKELYDFYAAKMFAVCLRYAK
                     NDMDAEDILQDGFVKIFTNLDKYRADGSFEGWLRRIMVNTAIEHIRKNKINNQDISEN
                     IENTVKDKYANALDKLFEKELVNIASTLSDGYKAVFNMYAIEGYSHKEIAKQLGITES
                     TSKSQFSRAKSILRNLLQVNNNQNVIAC"
     gene            complement(4220..5239)
                     /locus_tag="PLU36_05830"
     CDS             complement(4220..5239)
                     /locus_tag="PLU36_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HMZ46305.1"
                     /translation="MNLHYYTYNLPFEYPFEISGKRIKTHQPTLIVILELGNFYGVGE
                     ATAINYYNVTTEKMVDVIEKNKKMIEKFAFIQPERYWHYLHHLIPDSPFLTCALDMAA
                     WDLYGKMQKNNLYKLWNTDLENYPATSYTIGLDSLDKMVEKVKAKPWNSYKIKLGVEN
                     DIEIIEAIRKHTNAKIRIDVNGAWQTKEAIDKINILKDFNIELIEQPLTKDNWEGMKT
                     LFNQTTIPLFADESCVHEEDVVKCNGYFHGINIKLTKCSGITPAIRMIEEAKSLGLKV
                     MMGSMNESSIGTSAIANFLPQLDYVDMDGPLLLSKDLAIGLQISPSKVTLDGKLGLGI
                     QPTLF"
     gene            complement(5336..6937)
                     /locus_tag="PLU36_05835"
     CDS             complement(5336..6937)
                     /locus_tag="PLU36_05835"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HMZ46306.1"
                     /translation="MKFKIFSAVIIAGSISLFSCQKKTLELQPFSSFSDQSAFSNADR
                     CQLALNGVYDAAQSGFYAGGAVRGYPFGAANIEQGEMRGEDMLNQATFFAITYAATYN
                     SASPNNGYMFQTIYSMVNKANLVIDGVKAAGTGGVITAAQALQFEAECRFLRALGHHE
                     LVINFSRPFADGAGNKMGIIYRDFAINSDASSAAARAIKRSTITVADNYTKILEDLDF
                     AETNLPATQTGAGLSTYRATKAAAIALKMRVKMHKGDWAGVIAEGAKIVTGTAPNFLS
                     PIGGWKLTAAPDGPFANNTSTESVFSIKNDATDNPGVNGALAQMFGDPALSARGLVRV
                     SPIMWSEADWKCNDLRRTALYRTAGGPVNAVYSTKFRDYSTTSDGTPQIRYAEVLLML
                     AEANARTSTIVSSTAVDLLNAVRNRAVTTPGDQFTLLSFATKNDLIAAILKERRIELL
                     GEGKRWGDIHRLALDPDFAPIAGGGIPAKVGTGGATAAMFNCAGGVTITKSILAIPYS
                     DFKFVWPIPIEETQQNANYDQNPNY"
     gene            complement(6964..>7318)
                     /locus_tag="PLU36_05840"
     CDS             complement(6964..>7318)
                     /locus_tag="PLU36_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ46307.1"
                     /translation="NILKRWTQAGDVTTVPRLWYNRDNFTNLNQQAVDRFVESGNFIR
                     LDNLQISYDVESKHITKLTNSYVKSFRVFVQGQNLLLITKYTGIDPDNIDSRGLDYNT
                     VPQARSISFGVNVGF"
BASE COUNT         2400 a         1144 c         1064 g         2710 t
ORIGIN      
        1 ttttgtagct tcccctctaa taattccaat actttttaat cctgctgact tgcaagcata
       61 agctgtagca acaatatgat tactgtaagt gccaccaaat gtggctaatg tatctttacc
      121 tttttttatg caatcttgta tgaaatactt tagtttaaac catttattgc cactaacaat
      181 taaatgtatt tcatctgttc ttaaaacttc aacttcaata ttattaaaca aattagattg
      241 tagtttttga atggctacat tcatataaaa ttattctttt aaaagggtag gggtataatt
      301 tgtattgtgt agaattactc taaaagttta ttacgcattg caaacataat aaggcctgca
      361 atagttttta cgccagcctt ttgtttcatt gcttgcctaa tggtttcaat ggttctaggg
      421 cttagaaaaa cttctttaga aatttcttcg gtggttttat cttcactaat aagttttatt
      481 attcttaact ctttttcatt aaactgtgct ggtgtaggat aaaaacgcct aactgatttt
      541 ttatgttgaa gttttaaaag tacagcagta tttactaaat cgttaaagta aaaatcttcg
      601 ttcatacagg ttaatattgc ctgataaatt tcttctgggt ctgttgtttt agtaaggtag
      661 gagttagcac ccatttccat cattttagta ataatttcct gatcatcata catacttaat
      721 acaataattt ttaaattttc gtaagtatgt cttattaatt taattgcttc aattccatcc
      781 atttcaggca ttcttatatc catcaataat acgtctggct ccttcatttt tagtttatga
      841 gtcatgtctt ttccatcttc tgcttcccat aaaattttaa tatactcttt atcttttaag
      901 gtgttcctta aaccatctct aaaaatttta tgatcatctg ctaatgctaa cctaattatt
      961 ttttcagttt tcatattaag gataatatta agaacccttt tatcgaattg aattattcat
     1021 ttttttactt tatacaaacc aaactaaggt catttatttt ggtttattca acttaaatgt
     1081 gcataaatac ctgcatcgta atttttctat gaacatttac tagaaatgct agtattctta
     1141 ctaaatttga aaaacgtaaa attacgttaa caacttagtg taattacgat aaattgaatg
     1201 ttgattttac taaattattc ttctgaaagt attatcagca ttgaatttca tcaaatttaa
     1261 gcttgaatta ctattaataa ttagggttgt ggataaataa tttataaatg aagtttatgc
     1321 aatatttaat taacacccca aaaaatctaa cacaatggca aaagaattaa ttgtagtaaa
     1381 aggaagggtt atagaacaaa atcgttcttt tttacctaat gttggcgaag atattggcta
     1441 cgagcttgga gccaaaatga ttaaacgttg gcataccgaa aatccggatg atgttttagc
     1501 ttcttttaca ggtagagaat taattgaaaa aattcttgca caacctggtt gtactggtat
     1561 aagaatgttt aatgccataa acgatttagg aattaagcaa ttggttttag ttggggccga
     1621 taaaaatggt aacaatattt tagaatatac cacaattggt aacaatggtg aggtaataaa
     1681 acataaagga attgttgggg atagaagtgc aatttgccct ccaagttgtg gacaaggtga
     1741 tatggattta gattggtcat cttaattata taaaggttta ttatgtaaac attatttgct
     1801 tatttttgag caaattgttt agcattgaac tatttatttc atatttcagt tttctctcct
     1861 ttaataccca ttttgttttt tgtgctgttt aaatttaaca gtaagcaaag agcaaaatgg
     1921 gtaatttttt tattattggt aatcagtcta ataactgata tttggtgtta taacttagca
     1981 agaaataata ttaaaacaac ttggattatc aacatatttt ctgttattga gtttgctttc
     2041 ctcagttact tttttacctt aattatctca ctcaaaaagc aacaaatttt tatttggtta
     2101 tgttatgctg gctttttatt gtattggtta gtgcagaata tttttgttga gagtatagaa
     2161 atttttgatt atcaatctca agctattgaa tttttgttat tacttatttg ttgtttgttg
     2221 tatttgtttc aaagagttaa aaatgtaaga ggagaaattt tatataacac ataccaattt
     2281 tggattgtag cagctttatt aatgtattgt gccggtactt tttttggctt tttaagttct
     2341 gtgtctccag aagaaagaga ctttgataca attgtatatg aatatttatc aaggggtgga
     2401 aatattctaa aacatatttt aataactatt gcattttgca ttaaccccgc taatcaaaaa
     2461 aatgagcacc ccaataaaaa tagtatttat tatataaaag ataacttagg aaaagactaa
     2521 taatttatgc aattactaat aaattttact caaatacttt taactacaca aatcacccct
     2581 gttttatttt taggaacaat aggaatgata ttacttgcta catcaataat attatttgct
     2641 atttttcatc aacgcagggt aatgcgtttt cagcagagaa tacaacaaat ggaagtagag
     2701 catcagaaaa tgttattaaa agcttctata aaattacagg aagaagaaag aaccaggtta
     2761 gcagcagatt tacatgatga tgcaggccca ttattagcaa cagcaaaatt atacctcaac
     2821 gataattttg ttaatttaga taaaactgca caaatgcatt ctttatttaa tgctaaacaa
     2881 attatagatg atactattca attaattaga aacatttctc acagtttaat gccaccaact
     2941 ttaaaaaatt ttggtttaag tagtgctgtg aacgattttt ttcaaaaaat aagtaattca
     3001 ggttctttaa atgctagttc aaaatttcac gattataaaa caaggctaaa atcagataaa
     3061 gaacttattt tatttagagt tattcaagag ttagtaaata atgtacttaa acatagtaaa
     3121 gcttcattta tacatttaac ccaacacgat gttgaagaaa aagtaatttt aagattgcac
     3181 cacgatggat ttggattaac ccaagaagaa tttttaagat ataccaacga aaaagaaggt
     3241 ttaggtttaa agaatataca aagtagatta agggttattc atggtgatat tttatatgag
     3301 aaagatagtt ctcaatcttt atataaagta accatagaaa taccaagaga ggagggagag
     3361 gagataaaat aaacctaata atttatagta attagtatgt tctaattatc ttataatcac
     3421 attgttgttt aatattaata tagtaacttt ttgtgtatat tgctaaaaaa gcctcattta
     3481 gttttaaatg aggctttttt aatgagttat taataataat taacaagcta taacattttg
     3541 attattattt acttgtaata aatttcttaa tatagatttt gctctgctga attgagattt
     3601 actggtactt tctgttattc ctaattgttt ggcaatttct ttatgcgaat agccttcaat
     3661 agcatacatg ttaaaaacag ctttgtatcc atcgcttaaa gtagatgcta tatttaccag
     3721 ttccttttca aacaatttgt ctaatgcatt ggcatatttg tctttaactg tattttctat
     3781 attttcagat atatcttgat tatttatttt attcttacga atatgctcaa tagcagtatt
     3841 taccataatt cttcttaacc aaccttcaaa agagccatct gcacggtatt tatctaagtt
     3901 ggtaaatatt ttcacgaagc catcttgtaa tatgtcttca gcatccatat cgtttttagc
     3961 atatcttaaa caaactgcaa acattttagc tgcatagaaa tcatacaact ctttttggct
     4021 ttttctgcaa ccttcaatac atttttgaat ttgtgtattt tctgaattta taaattgtac
     4081 ttgcataact taatggtttg tgctacctgt aaaaccatta aaatgccaaa attgtaaatg
     4141 attgattatt aagtagttaa attaaagata gtctagttta aaatcccctt gaaggactac
     4201 ctttcccaaa atgagaagac taaaaaaggg taggttgaat acctaatcca agttttccat
     4261 ctaaagtaac ttttgaagga gaaatctgta agccaatagc taaatcttta gaaagtaata
     4321 aaggcccatc catatctaca taatctaatt gaggtaaaaa attagcaatt gccgaagtgc
     4381 caatgctgct ttcattcata cttcccatca ttacttttaa acctaatgat tttgcttctt
     4441 caatcattct aatagcagga gtaattccac tgcatttggt aagcttaata ttaatgccat
     4501 gaaaatatcc attacattta actacatctt cttcatgcac acaactttca tctgcaaata
     4561 ggggaatggt ggtttggtta aataatgttt tcattccctc ccaattatct ttggttaatg
     4621 gttgctcaat taattcaata ttaaagtctt ttaaaatatt aattttatct attgcttctt
     4681 tggtttgcca ggcaccattt acatcaattc taatttttgc attagtatgt tttcttattg
     4741 cttcaataat ttctatatca ttttctactc ctaatttaat tttataacta ttccacggtt
     4801 ttgcttttac tttttcaacc attttatcta aactatctaa gccaattgta taggaggtag
     4861 ctgggtaatt ttctaaatct gtattccata gtttgtataa attatttttc tgcattttac
     4921 catataagtc ccaagcagcc atgtctaaag cacaagttaa aaaagggcta tcaggaatta
     4981 aatggtgtaa ataatgccag tatctttctg gttgaataaa agcaaatttt tctatcattt
     5041 tcttattctt ttctattaca tcaaccatct tttcagttgt tacattataa taatttattg
     5101 ctgttgcttc tccaactcca taaaagtttc ctaattctaa aattacaatt aaggttggtt
     5161 ggtgagtttt aattctcttt ccagaaattt caaaaggata ttcaaaaggc aggttataag
     5221 tataataatg taaattcata aatatgcaaa tgtattattt aaaatattat caactctata
     5281 aaacaaaaag ctgcacctaa taggtgcagc ttaaaattat aaccccttga aaaaattaat
     5341 aattagggtt ttggtcgtaa ttagcgtttt gttgagtttc ttcaattggt attggccaaa
     5401 caaatttaaa gtcgctgtaa ggtatcgcta aaatgctttt agttatagtt acaccacctg
     5461 cacaattaaa cattgcagca gttgcaccac cagtaccaac tttagcagga ataccaccac
     5521 cagcaattgg agcaaaatct gggtctaatg ctaatctatg aatatccccc cagcgtttac
     5581 cttctcctaa aagctcaatt cttctttctt ttaaaattgc agcaattaaa tcgtttttag
     5641 ttgcaaaact aagtaatgta aactgatctc caggagttgt aactgctctg tttcttactg
     5701 catttaataa atctacagca gttgaagaaa caattgttga agttcttgcg ttagcctctg
     5761 ctaacattaa taaaacttct gcatatctaa tttgtggtgt accatcagat gtagtggagt
     5821 agtctctaaa ttttgttgaa tacacagcat taacagggcc gccagcagtt ctgtaaagag
     5881 cagtacgacg taaatcatta catttccaat ctgcttcgct ccacataatt gggcttactc
     5941 taactaaacc tctagcactt agtgcagggt ctccaaacat ttgagcaaga gcaccgttta
     6001 cacctggatt gtctgttgca tcatttttaa tagagaatac actttccgta gaagtgttat
     6061 tagcaaaagg tccatcaggt gctgcagtta atttccatcc accaattggg cttaagaaat
     6121 taggtgcggt accggtaaca atttttgcac cttcagcaat aacacctgcc caatcacctt
     6181 tgtgcatttt aaccctcatt ttaagagcaa ttgcagctgc tttagttgca cggtatgtac
     6241 ttaagccggc tcctgtttgt gtggctggta aattagtttc agcaaaatct aaatcttcta
     6301 aaattttagt ataattatct gcaacagtaa tggtagaacg tttaattgct cttgcagcag
     6361 cagatgatgc atcggaattg attgcaaaat ctctgtaaat aattcccatt ttattacctg
     6421 caccatcggc aaaaggtctt gagaaattta ttacaagttc atgatgccct aaagctctta
     6481 aaaatctaca ttcagcttcg aattgtaatg cttgtgctgc agtaataacg ccacctgtgc
     6541 ctgctgcttt tacaccatct atcactaaat tggctttgtt taccatacta taaatagttt
     6601 gaaacatata cccattgtta ggagatgcac tattataggt tgcagcataa gttattgcaa
     6661 aaaatgttgc ttggttaagc atatcttctc ctctcatttc tccttgttca atattagctg
     6721 caccaaaagg atagcctctt actgcaccac ctgcataaaa gccagattgt gcagcatcgt
     6781 aaacaccatt taatgctaat tggcatctat cggcattaga aaatgcagat tggtctgaaa
     6841 atgaagaaaa aggttgtagt tctaaagttt tcttctggca ggaaaacagg cttatactac
     6901 ctgctataat gactgctgaa aatattttaa atttcatatt taatgttttt aagcgttata
     6961 taattaaaag ccaacattca ctccaaaaga aattgatcta gcttgtggta ctgtattgta
     7021 gtctaagcct ctagaatcaa tattatctgg atctattcct gtgtattttg taattaataa
     7081 taaattctga ccttgtacaa atactctaaa tgattttaca taagagttgg taagctttgt
     7141 aatatgtttg ctttctacat catagcttat ttgtaaatta tctaatctta taaaattgcc
     7201 actttctaca aatctatcca cagcttgttg gtttaagttg gtaaaattat ctctgttata
     7261 ccataaacgt ggtactgtag ttacatctcc tgcttgtgtc caacgtttta aaatattt
//
LOCUS       DAOQPK010000094         7313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_5713_length_7313_cov_4.651006, whole genome shotgun sequence.
ACCESSION   DAOQPK010000094 DAOQPK010000000
VERSION     DAOQPK010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7313
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5713_length_7313_cov_4.651006"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..297
                     /locus_tag="PLU36_05845"
     CDS             61..297
                     /locus_tag="PLU36_05845"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HMZ46308.1"
                     /translation="MWQEIENKLYKKFSFKNFSEAFAFITQVALEAEKMNHHPNWSNS
                     WNIVEIWLYTHDMGNKVTEKDILLSKKIDAIISK"
     gene            352..2166
                     /locus_tag="PLU36_05850"
     CDS             352..2166
                     /locus_tag="PLU36_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ46309.1"
                     /translation="MQEPNKKKVFDYSLLVRIYSYVKPYKHWFYFSLVLAILIAILAP
                     IRPYLIQLTIDKATGKNIIIPTWLQFIFPSKNLNIAAQLVIWITIFQIVFIIFETIIR
                     FVFSYLTAKLGQHIVKDLRVSVFKKILHLRLRQFDKTPIGTLTTRTINDIESINDIFS
                     DGLIPIISDLLTIIFTVATMFWINWQLALIALIPFPFIIIATYYFKESVNASFIRVRN
                     AVANLNAFVQEHLTGINIVQAFAAEEKEFKKFEAINQEHRSANIKAIFAYSVFFPVVE
                     LVLAVSIGLTVWFIAGKQLNPGLLIAFILYLNQIFKPLRIIADKFNVLQMGMIAAERV
                     FKVLDNKDVEPTNNTSANILQLKGDITFNNVWFAYKPQQYVLKNISFNVQQGETVALI
                     GHTGSGKTSIISILNKLYDIEKGEILIDDFNIDKINISTLRKNIAVVLQDVFLFAGTI
                     KDNITLENKLITNEKVIEAAKLIGVHDFIMKLPDNYNFNVGERGNSLSMGQKQLISFI
                     RALLYNPSILILDEATSSIDSESEILIQKATEQLIKNRTSIIIAHRLSTIKKANKIIV
                     LDNGEIKEIGSHEELINKNGYYTKLYNLQFNINEKILI"
     gene            complement(2158..3129)
                     /locus_tag="PLU36_05855"
     CDS             complement(2158..3129)
                     /locus_tag="PLU36_05855"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HMZ46310.1"
                     /translation="MKKIKYYYNTHTLRFEKIEVPLHVKLLRVFGFVAASLVSASVIF
                     FIAGRLIESPEAKLLRIQNDDWKDNYAVLQERTRQLEMQMQELEDRDNSVYRSIFESQ
                     PIPDSARVKDNEVKQEVKLIQKMGETDLVKSMSAQLNNLSLRIAYQSKSFNEIDDMVK
                     NKEKLLAAIPAIQPVSNKRLKRIASGFGNRIDPVYKVTKFHAGLDFTAPIGTPIYATA
                     DGTIKTANFNAGGYGNHVIINHGYGYETLYGHMVKIKARQGQSVKRGEVIGYVGSTGK
                     STGPHCHYEVHRNGHPVDPIYYFYNDLTPEQYDRLLKLAAASNQSLD"
     gene            3316..4140
                     /locus_tag="PLU36_05860"
     CDS             3316..4140
                     /locus_tag="PLU36_05860"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ46311.1"
                     /translation="MISLIKTEWLKLKKYRIFWWMLVIVALTYPGINLMFYKVYSSIT
                     AKEGMEGSLMKMLIGNPFAFPEAWHTIAYFSSWFVLVPSILIIMIISNEYTYKTNRQN
                     VIDGWSRKEFILSKLFIVIIVSIIATLMFTIITIILGLNYSTEIQATRWNEQIKYIFL
                     FLLQTFSQLSLAFFVGFIFKRSFIALGVFFFYLIIVEPSLYFLIDKYFELPTLAKLLP
                     LELSDRLIPPAAFLGKFNKEAYEQSLDNINLHILYTVIFTFLIWLFSIKIYNKRDL"
     gene            complement(4153..5514)
                     /locus_tag="PLU36_05865"
     CDS             complement(4153..5514)
                     /locus_tag="PLU36_05865"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMZ46312.1"
                     /translation="MKNFFLTLLFITSYSAIYAQPKKVVADKIIAQVGDKIILRSDVI
                     NAIEDYKRQGQAASLPPNPECAFLEAQLIQKALILQAEKDSLTVSDEEIEAALDNQIR
                     GFIQMYGSKEMLEEYSGRTIYQLKEDFRGPFRERKLADLMKSNIVDGVKITPAEVKVF
                     FNKIPKDSLPYYESEIEISQIVTIPKANKEVEDYVIKQMYDYKKLVENGQKKFEALAK
                     LYSEDPGSKENGGMYNINRNEKFWDKDFLQGAFRLKEGQISPVIKSKFGYHIIQMVNR
                     AGDDAVIRHILRIPPITDEEINLAKQKLDTIRKNIKEGKLTFSEAVNKYSDDEESKFN
                     GGAKIGADGSTFLSIDALDKEMVIAIKDLKEGDLSNPIVYEDRGLKKVRIVFLKSRTE
                     PHIENLREDYNKVAARALEEKKQHILEQWFKDHLPLYYITIDKEFTNCADLLDWWKFA
                     KSR"
     gene            complement(5737..6087)
                     /gene="rplS"
                     /locus_tag="PLU36_05870"
     CDS             complement(5737..6087)
                     /gene="rplS"
                     /locus_tag="PLU36_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HMZ46313.1"
                     /translation="MNQAVQFVHEQLTATKNHPKFKAGDNVTVNYKIVEGGKERIQSF
                     RGDVIKIQGNGSTATFTVRKISDGVGVERLFPFASPNVDAIVLNRVGKVRRAKLFYLR
                     ERSGKSARIKEKRI"
     gene            complement(6185..6823)
                     /locus_tag="PLU36_05875"
     CDS             complement(6185..6823)
                     /locus_tag="PLU36_05875"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HMZ46314.1"
                     /translation="MSKPFLDYLSDSELLIDKQYQPQTEKLLEYYELYKLVKNLNGSI
                     LKCGVNNNETFSYFSFFKKTNQYNTNQPLVVFEKNPSVFEPALVNNETVITVKSSEDI
                     ISSRKNIALKSKKEDIDFIPGNVTNALPTYLINNPELKIALLVIDLDNYEQTLAALQY
                     LYPRIVSNGILIISNYYKKAEDTAAIKEYFANQSVIIRNYSKSKGLHYIIKD"
     gene            7028..>7313
                     /locus_tag="PLU36_05880"
     CDS             7028..>7313
                     /locus_tag="PLU36_05880"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HMZ46315.1"
                     /translation="MQLPFFYESNLTEQSNLFQLSSTSFKHAIQVLRLQKEDAIHITN
                     GKGLLCRAIILNADKKQATVKVEAIKFIEKEVVKTCIGISLLKNNNRFEWF"
BASE COUNT         2483 a         1082 c          974 g         2774 t
ORIGIN      
        1 ggcagagcta accaatgagc aaataaaaac caaaggcaat atgatatttt aatttttttt
       61 atgtggcaag aaatagaaaa taaattatat aaaaaattta gcttcaaaaa tttttctgaa
      121 gcatttgcat ttataactca agttgcttta gaagcagaaa aaatgaatca tcatccaaac
      181 tggtctaact catggaatat tgttgaaatt tggctataca cccatgatat gggcaataag
      241 gttacagaaa aagatatttt actctccaaa aaaatagatg caattattag caaataattc
      301 ttaagatatt tatttaagaa aatttttgcc atacttacat ttgtagatag tatgcaagag
      361 cctaataaaa aaaaggtatt tgattattca ttattagtta gaatttattc ttacgtaaag
      421 ccttataaac attggtttta ttttagctta gttctggcaa tactaatagc aatattagct
      481 ccaatacgtc catatttaat tcaacttact atagataaag ctacaggtaa gaatataata
      541 attcctactt ggttacaatt tatctttcca tcaaaaaatt taaacatagc tgcacagttg
      601 gttatttgga taaccatttt tcaaattgta tttataattt tcgaaactat tatcagattt
      661 gttttttctt atttaactgc aaagcttggg caacatattg taaaagattt aagggttagc
      721 gtttttaaaa aaatattaca cctaagatta aggcagtttg ataaaacacc aataggcact
      781 ttaaccacca gaaccatcaa tgacattgaa agtattaatg atatttttag cgatggatta
      841 atacccatta tttcagattt gcttacaatt atttttacag ttgcaacaat gttttggatt
      901 aattggcaat tagccctcat tgctttaatt ccatttccat ttataattat agcaacctac
      961 tattttaaag aaagtgttaa tgcaagtttt atacgagtaa gaaatgctgt agctaactta
     1021 aatgcctttg ttcaagagca tcttacagga ataaatattg tacaagcctt tgcagcagaa
     1081 gaaaaagagt ttaaaaaatt tgaagcaata aatcaggaac atagaagtgc aaacattaaa
     1141 gcaatttttg cgtatagcgt tttttttcct gtagttgagt tggttttagc agttagtatt
     1201 gggcttacag tttggtttat tgcaggcaaa caacttaatc ctggtttatt aattgcattt
     1261 attttatacc ttaatcaaat ttttaaacct ttaagaatta ttgcagataa atttaatgtt
     1321 ctacaaatgg gcatgattgc tgccgaaaga gtttttaaag ttttagataa taaagatgtt
     1381 gaacctacaa acaatacttc tgcaaacatt ttacaactaa aaggagatat aacttttaat
     1441 aatgtttggt ttgcatataa accacaacaa tacgttttaa aaaatatttc atttaatgta
     1501 caacagggcg aaacagttgc attaattggg catacaggta gtggtaaaac atcaattata
     1561 agtatattga ataaactata cgatatagaa aagggcgaaa tattaataga tgattttaat
     1621 attgataaaa ttaacataag tacattaaga aaaaatattg cagttgtttt acaagatgtt
     1681 tttctttttg caggaactat aaaagacaat attacacttg aaaataaact tataacaaat
     1741 gaaaaagtta tagaagctgc taagctaatt ggtgtacatg attttataat gaagctacca
     1801 gataattata attttaatgt tggcgaaaga ggcaactctt taagcatggg gcaaaaacag
     1861 ctaatctctt tcatcagagc tttattatat aacccttcta tattaatttt agatgaagca
     1921 acatccagta tagattcaga aagtgaaata ctaattcaaa aagcaacgga acaattaatt
     1981 aaaaacagaa catctataat aattgcacat agattaagca ctataaaaaa agccaataaa
     2041 attattgttt tagataatgg agaaataaaa gaaataggct cgcatgaaga actgataaat
     2101 aaaaatggtt actacactaa actatacaat ttgcagttta atattaatga aaaaatttta
     2161 atctaaactt tgattgctgg ctgcagcaag ttttagtaac ctatcgtatt gttctggtgt
     2221 taagtcgtta taaaaataat atatcggatc tacagggtgg ccatttctat gaacctcata
     2281 atggcaatgt gggccagtgc tttttcctgt acttcctaca taaccaatta cctcacccct
     2341 ttttacactt tggccttgcc ttgctttaat ttttaccatg tggccatata aagtttcgta
     2401 tccataccca tgattaatta taacatgatt accatatcct ccagcattaa aattggcagt
     2461 ttttatagta ccatctgcag ttgcataaat aggtgtgcct attggtgcag taaaatctaa
     2521 cccagcatga aattttgtta ctttatatac agggtcaatc ctgtttccaa aaccactggc
     2581 aatacgtttt aaccttttat tacttacagg ttgtatagct ggtatggcag ccaataattt
     2641 ttctttattt tttaccatat catctatttc attaaaactt ttagattgat aagctatcct
     2701 taaggataaa ttattgagtt gtgccgacat gctttttacc aaatccgttt cgcccatttt
     2761 ttgaataagt tttacttctt gctttacttc attatctttt actcttgcac tatccggtat
     2821 tggttgcgat tcaaaaatac ttctataaac agaattgtct ctatcttcta attcttgcat
     2881 ttgcatttcc aattgccttg ttctttcctg caaaacagca taattatctt tccaatcatc
     2941 gttttgtatt cttaataatt tagcttctgg gctttcaatt aatcttcctg caataaaaaa
     3001 aataactgaa gcagatacca aactagctgc aacaaaacca aataccctca gcaattttac
     3061 atgcagtgga acttcaattt tttcaaacct aagtgtgtgg gtattgtaat aatattttat
     3121 tttcttcatt gccaagggct aaaattagta ttcttatttt gttttaaaaa gttcatttca
     3181 cacttaatca cattttctct cttgcaaata acaattattt aattaccatt taaaatgtat
     3241 ttagtattga ttgcaattta tttttctatt tcatttatat tcgtttatca ttcattaaag
     3301 cagtaaatgt attatatgat ttcattaata aaaacggaat ggttaaaact aaaaaaatat
     3361 agaatttttt ggtggatgct ggtaattgta gctttaacct atcctggtat taatttaatg
     3421 ttttataaag tttactcttc tataactgcc aaagaaggta tggagggaag tttaatgaaa
     3481 atgcttattg gcaacccctt tgcctttcct gaagcatggc atacaattgc ttatttttca
     3541 tcttggtttg tattggtgcc ttccatttta atcatcatga ttatctctaa tgaatatact
     3601 tataaaacaa acaggcaaaa tgttattgat ggctggagta gaaaagaatt tattttaagt
     3661 aaattattta ttgttataat agtatcaata attgctactt taatgtttac tattattact
     3721 attattttag gattaaatta ctcaacagaa atacaagcaa ccagatggaa cgagcaaatt
     3781 aaatacatat ttttattttt actacaaacc ttttcgcaac tttcattggc attttttgtt
     3841 ggttttattt ttaaaagatc ttttattgct ttaggtgtat tttttttcta tttaataatt
     3901 gtagagccca gcttatattt tttaattgat aagtattttg aattacctac acttgcaaaa
     3961 ttattgcctt tagaacttag cgatagacta ataccacctg ctgcattttt aggaaaattt
     4021 aataaagaag cttatgagca aagtcttgac aatattaact tacacatttt atatactgtc
     4081 attttcacct ttcttatttg gctatttagt ataaaaattt ataacaaaag agatttataa
     4141 aagaggtata aattatctgc tttttgcaaa cttccaccaa tctaacaaat cggcacagtt
     4201 agtaaattct ttatctattg taatatagta taatggtaaa tgatctttaa accattgctc
     4261 taagatatgt tgcttttttt cttctaatgc tcttgcagct actttattat aatcttctct
     4321 aaggttttct atatgtggtt cagttctgct ttttaaaaat acaattctca ccttttttaa
     4381 acccctatct tcatatacaa ttgggttaga tagatctcct tcttttaaat ctttaatagc
     4441 tataaccatt tctttatcta atgcatctat agataaaaag gttgagccat ctgcaccaat
     4501 tttagctcct ccattaaact tactttcttc atcatcgcta tatttattta cagcttcact
     4561 aaaagttagt ttaccttctt taatattttt tctaatagta tctaattttt gtttggctaa
     4621 gttaatttct tcatcagtaa taggtggtat tcttagtata tgtctaataa cagcatcatc
     4681 acctgctctg tttaccattt gtattatatg atacccaaac tttgatttta taactggtga
     4741 aatttgtcct tcttttaatc taaaagcacc ttgtaagaaa tctttatccc aaaatttttc
     4801 atttctgtta atattataca ttccaccatt ttctttactt ccaggatctt ctgaatataa
     4861 tttagctaat gcttcaaact ttttttgtcc attttcaact agttttttat aatcatacat
     4921 ttgcttaatc acataatctt ctacttcttt attagctttt ggtatggtaa caatttggct
     4981 aatttcaatt tcactttcat aatatggcaa actatcttta ggtattttat taaaaaaaac
     5041 tttcacttct gctggtgtta tttttactcc atcaacaata ttactcttca ttaaatcagc
     5101 taattttctt tctctaaagg gccctctaaa atcttctttt aattgataaa ttgttctacc
     5161 agaatattct tctaacattt ctttacttcc atacatttga ataaatccac gaatttggtt
     5221 atcaagtgct gcttcaattt cttcatcgct aactgttagt gaatcttttt cggcttgcag
     5281 tattaaagct ttttgaatta gttgagcttc taaaaaagca cattcggggt tgggaggtaa
     5341 acttgcagcc tgtccttgtc ttttataatc ttcaattgca ttaataacat cagaacgtaa
     5401 aattatttta tctcctacct gtgctattat tttatcggct acaacctttt ttggctgggc
     5461 ataaatggca ctataagaag taataaaaag tagggttaaa aaaaaatttt tcatttgttt
     5521 atcttttaat ctttcttgtt atgaaagaat ttatcttaca ataatttgct attatgcttt
     5581 tttgtaacaa actattttat ggctcttgta ttttatataa aagcaatgtt gtaaaaatag
     5641 caggagtttt taaaaataca gtttaattat aatgttttaa caaaataaaa aaagcggaaa
     5701 ttaaatattc cgcttttaaa aataagtata taataattag atacgttttt ctttaattct
     5761 tgcactttta ccacttcttt cacgtaagta aaataatttt gcacggcgta ctttaccaac
     5821 tctatttaaa acaattgcat ctacatttgg agaggcaaac ggaaataaac gctcaacacc
     5881 aacgccatct gatattttac gaactgtaaa agtagctgtt gaaccgttac cctgaatttt
     5941 aatcacatcg ccacggaaac tttgaatacg ctctttacca ccttctacaa ttttatagtt
     6001 tacagttaca ttatcgcctg ctttaaactt aggatgattt tttgtagctg ttaattgctc
     6061 atgtacaaat tgaactgctt gattcataac actttctttt tttcggattg caaaggtaag
     6121 gggttgtaat taaatagaaa aaataaatag gctgtttttg taaatattat tgagtaaaaa
     6181 gaaattaatc tttaataata tagtgtaacc ctttactttt tgaataatta cgaattataa
     6241 cactttgatt tgcaaagtat tctttaattg cagcagtatc ttctgctttt ttataataat
     6301 tactaattat taaaataccg ttgcttacaa ttcttggata taggtactgc aaagcagcca
     6361 aagtttgttc ataattgtct aaatctatca ctaataaggc tattttaagc tctgggttat
     6421 taataagata agtaggtaaa gcgttagtta cattgccagg aataaaatca atatcttctt
     6481 tcttactttt tagtgcaata ttttttctcg atgaaattat atcttctgaa ctttttactg
     6541 taattactgt ttcattattt actaatgcag gttcaaatac agaaggattt ttttcaaata
     6601 caaccaatgg ttgattggta ttatattgat tggttttctt gaaaaaggaa aaataactaa
     6661 aagtctcgtt attgtttaca ccacatttta aaatagagcc attaaggttt tttactaatt
     6721 tatacaattc gtaatactct aatagtttct ctgtttgggg ttggtactgc ttatctataa
     6781 gcaattcaga gtctgacaag taatctaaaa aaggctttga cattaaaatc aattgttgat
     6841 tgtttaacaa ttggtttttt ctatagagat aattgggtac ttaaaatgat taaatttcgc
     6901 ttaaataacg gattacttac tatgatgaat ttttatatat aaaggttgca aaagagtata
     6961 aaaaatcatc tgtttttact taacatttct gatacaacta aaccaaactg taaataaatt
     7021 tgccaatatg caattaccat ttttttatga gtcaaattta acagaacaaa gcaacttatt
     7081 tcaattaagt tctacttctt ttaaacatgc tattcaagtt ttaaggctac aaaaagaaga
     7141 tgctatacat attaccaatg gcaaaggttt attatgcagg gctattattt taaatgcaga
     7201 taaaaaacaa gctacggtta aagtagaagc tataaagttt attgaaaaag aggtagttaa
     7261 gacatgtatt ggtatttcat tattaaaaaa taataatagg tttgaatggt ttt
//
LOCUS       DAOQPK010000095         7307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_5720_length_7307_cov_4.675676, whole genome shotgun sequence.
ACCESSION   DAOQPK010000095 DAOQPK010000000
VERSION     DAOQPK010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7307
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5720_length_7307_cov_4.675676"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PLU36_05885"
     CDS             complement(<1..502)
                     /locus_tag="PLU36_05885"
                     /inference="COORDINATES: protein motif:HMM:NF017936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HMZ46316.1"
                     /translation="MLAAKDYIEHYHLTPHPEGGFYKENYRSNLVLKQHSLPVTFTGN
                     RNVSTAIFFLLEQGNFSAFHKIKSDECWHFYAGQTLLVYVIHATGNLEIIRLGNNIEM
                     GETFQYIVPANCWFASEPAERTTFSFVGCTVAPGFDFDDFELAKAAHLMQLYPEHETI
                     IKRLCRC"
     gene            complement(532..1188)
                     /locus_tag="PLU36_05890"
     CDS             complement(532..1188)
                     /locus_tag="PLU36_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ46317.1"
                     /translation="MSDKIKIIITDDHALFRQGVKAALSFRPDIEFLGEAENGMQLLN
                     MLKFLQPDVILLDIQMPIMDGIATLPEVKKVLPNVKVIMLTMHNEITLITKLMSIGAN
                     SYLTKDSESDVIYDAIKTCYENEFFFNEFTNRAMLSELRTQTAAPVKSDENAELSDKE
                     LQVLKLMCDEKSTKEIADIVEISPRTVEAIRDRLKIKTGAKSTAGLILYAVKHGLIEA
                     "
     gene            complement(1340..1831)
                     /locus_tag="PLU36_05895"
     CDS             complement(1340..1831)
                     /locus_tag="PLU36_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46318.1"
                     /translation="MRFIKLALISFLLLFIVVTAIGLLLPSTVIVSRAIDINAPKDSV
                     YNKVKNIYGWREWIAGMDKPEVNITSPTEANLFGTKVNITNINDTVITSTWLGRKGSL
                     QESVIRIIHYQNTPQIVVQWQFTESLKWYPWQRFGSMMNEAVTGKQLENNLAALKMLV
                     EKK"
     gene            complement(1854..2945)
                     /locus_tag="PLU36_05900"
     CDS             complement(1854..2945)
                     /locus_tag="PLU36_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ46319.1"
                     /translation="MQYKYSQLTALIAITKASLLAIIKNPGSIFFSLVFPLVFIWIFG
                     SFGNGGLNKINIAFSNQSDTNNAVFAILKNLPQIKIATYSNEALQKADLEKGNLAAII
                     KIASNDTANKHQYLINVTATNATSAEVAQLIPILENISIKINPDFKNYIQIEKNIYSI
                     RAYRQIDFILPGQIGFSILFSTLFGIAFTFYNLREQLILKRFYASPVNKLNILTGIGF
                     SRLFFQLINVVVLIIVGRFWLGFTLINGLVTFLEMILITFLMLIVLMGVGLIISSVVK
                     TDATISLLINLFALPQMLLSGTFFPITVFPDWMQNICSIFPLTHFNTAMRKISFEGLG
                     LVSIWQHIAVIGLWGLVVYILIYKIFKWE"
     gene            3108..3605
                     /gene="folK"
                     /locus_tag="PLU36_05905"
     CDS             3108..3605
                     /gene="folK"
                     /locus_tag="PLU36_05905"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HMZ46320.1"
                     /translation="MDKAKKEYIVYLLTGSNIGNKKENLYHAKHLVDKHCGNVSNHSP
                     IYQTAAWGLTNQPSFYNQALQLHTFLEPENLMQTLLNIEEMLGRVRKEKMGPRIIDID
                     ILLIENYTSNTNLLQVPHIALPFRKFALIPLADIAATVLHPILKITIAELLTKCKDDL
                     DVQKI"
     gene            3645..4277
                     /locus_tag="PLU36_05910"
     CDS             3645..4277
                     /locus_tag="PLU36_05910"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HMZ46321.1"
                     /translation="MKHHFITVEGNIGAGKTTLTHLLAKHFNARIILEEFADNPFLSK
                     FYDNPKQYAFPVELFFMAERYKQMKDMVHTKDLFQTVTVSDYLFTKCLLFAKVTLPEE
                     EFRLYQKLFDIIHQQLLFPDILIYLHAPVQKLQENIKKRNREYEQQIKDEYLYNLQET
                     YTSYIKQHNIKTIFIDASNADFLGNEKHFQVVLNALEKDIDSGQHYFSLP"
     gene            complement(4274..4909)
                     /locus_tag="PLU36_05915"
     CDS             complement(4274..4909)
                     /locus_tag="PLU36_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HMZ46322.1"
                     /translation="MTYLIILSIAFGISFLGQIVLSNMNMAATQLSVQEGFNKAWKFG
                     IGVAIVEIIYLRLALTGMSLVTQYKYIFKLLGWITVVVFLILGVLAFISAYKQVVEKK
                     GLLINNKINRFLLGVTISALNPVQIPFWFLWSTQLIQNNVLQTNFHQFNFFCIGAGLG
                     TLAGIGVYIHGGNWAINKMKTSNKTLNLIMGVVFFITAIIQLYRIIYKPWI"
     gene            complement(4951..5997)
                     /locus_tag="PLU36_05920"
     CDS             complement(4951..5997)
                     /locus_tag="PLU36_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46323.1"
                     /translation="MKKILQKIADWEQWNFYVIYTPLVFVWLYYFIRTGKFWYFSNVN
                     PSLQFSGLEGETKKEMFEQLPLHTFPTTIYIKPACNFMEVVQQVNKAALHYPVAVKPD
                     IGTKGLCFRKIENEEQLKQYHATVPFEYLVQSFIEYPLELSVFYIRYPNSTKGKITGL
                     IAKEYLQVKGNGKQTLLELIQQHPKAKFRLNELTVKHAEHLQDIIENNEPYFLSITGN
                     HNRGATFVNLYQEIDQQLTNVFDEISNYTKHFYYGRFDIKTTNLKDLKAGKNISILEF
                     NGTGAEPNHIYDCNMSYFKALKTIAAHWADMYNIGRINYHKGIPYISFKQGRKFLADA
                     FTAYAVLEEYEKIC"
     gene            complement(6084..7139)
                     /gene="hisC"
                     /locus_tag="PLU36_05925"
     CDS             complement(6084..7139)
                     /gene="hisC"
                     /locus_tag="PLU36_05925"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HMZ46324.1"
                     /translation="MAFNINHLLRPNIEKIVNQKNNISKNEIKISLHKNENSLGSPLK
                     KWYNRYPDDEQNNLKQAIAALKNVTTENVFLANGTNESIEILYKCFCAPEKDNVIICP
                     PTYSQFENFADIYNIEVRKAVLLENFQLDLITLENLVDTHTKIIWLCSPNNPTGNSLN
                     LNDIEFVLNNFNGLVVVDEAYINFSAQKSLTALLKEYDNLIILQTFSIAWGLAGLRLA
                     IALSNPNTITILNKVKNTNTISTVSKETALKAFEEIGQVNDMIKLLVEMRIALADVFK
                     QIPFIIKVHDSDANFILIEVDNASKLTDYLLSVQIAVKNVSHLPNCHNCIRITVGNEK
                     ENTDLVDALSNYLEMHY"
BASE COUNT         2608 a         1167 c          956 g         2576 t
ORIGIN      
        1 agcatctgca taacctttta attatagttt catgttcagg ataaagctgc attaaatgtg
       61 ctgctttagc taattcaaaa tcatcaaaat caaaaccagg agcaacggta caaccaacaa
      121 aactaaatgt tgtacgttct gcaggctcgc ttgcaaacca acaattggca ggtacaatat
      181 attgaaaagt ctctcccatt tcaatattat ttcctaaacg aattatttct aaatttcctg
      241 ttgcatgtat tacatacacc aacaaagttt gccctgcata aaaatgccaa cactcatcgc
      301 ttttaatttt atgaaatgcc gaaaaatttc cttgttctaa taaaaaaaat attgctgtgc
      361 ttacatttct gttacctgta aatgttactg gcaaactgtg ctgctttagc actaaattag
      421 agcgatagtt ttctttataa aacccacctt ctggatgagg tgttaaatgg taatgctcta
      481 tataatcttt tgctgctaac aactataaag tttaagttat gaaagaagag tttaggcttc
      541 tattaagcca tgttttacag catataaaat taagccagca gtacttttag ctcctgtttt
      601 aatttttaat ctgtctctaa tagcttcaac tgttcttgga ctaatttcta caatgtctgc
      661 aatttcttta gtgctttttt catcacacat taactttaaa acctgcaatt ccttatcgct
      721 cagttctgca ttctcatcac tttttactgg tgctgctgtt tgggttctca attcagataa
      781 cattgctcgg ttagtaaatt cgttaaagaa gaattcgttt tcgtaacaag tttttatggc
      841 atcataaata acatcactct ctgaatcttt ggttaaatag ctattggctc caatactcat
      901 tagcttagta attaatgtaa tttcattatg catggttagc ataattactt ttacattggg
      961 taaaactttt tttacttcag gtaatgtagc aattccatcc ataattggca tctgaatatc
     1021 caacaaaatc acatctggtt gcaaaaattt aagcatgttt aaaagttgca ttccattttc
     1081 agcttctcct aaaaattcta tatctggcct aaaacttaaa gctgctttta caccttgtct
     1141 aaacaaagca tggtcatctg ttataattat ttttatttta tcgctcatag ctataacaaa
     1201 tatataaact tttggaaagg atagtaaaaa tatcaaaaaa aaccttccat tcttacaagg
     1261 aaaatgtaat aaaaaataag aaactactaa gtattaatac taatcattaa agtaaacaat
     1321 aaagtgttta ctcatttttc tacttttttt ctacaagcat ttttaaagca gctaaattat
     1381 tttctaactg tttgcctgta actgcttcat tcatcatact accaaaacgt tgccaaggat
     1441 accattttaa agattctgta aactgccatt gaacaactat ttgaggtgtg ttttggtaat
     1501 gaattattcg tataacactt tcttgtaggc ttccttttct accaagccag gttgaggtaa
     1561 taacagtatc gttaatgttt gttatattca ctttggtgcc aaataaatta gcttctgttg
     1621 gcgaagtgat attcacttca ggcttatcca ttcctgcaat ccattctctc caaccatata
     1681 tgttttttac tttattataa acactgtctt taggggcatt tatatcaata gccctagaaa
     1741 ctataactgt agagggtaat aataaaccaa ttgcagtaac aacaataaat aaaagcaaaa
     1801 aacttattaa agccaatttt ataaaacgca taataaaaat ttgaataatg aatttactcc
     1861 cacttaaaaa ttttataaat taaaatataa actaccaaac cccataaacc aattactgca
     1921 atatgctgcc atatacttac taaacctaaa ccttcaaaag atattttacg catagcagta
     1981 ttaaaatgtg ttaaaggaaa aattgaacaa atattctgca tccaatcagg aaaaacagta
     2041 attggaaaga aagtgccact taacaacatt tgcggtaaag caaataaatt aataagcaat
     2101 gatatggttg catctgtttt aactacacta gatattatta agcctacacc cattaaaaca
     2161 ataagcatta aaaaggtaat taaaatcatt tctaaaaaag taaccaagcc atttattaat
     2221 gtaaaaccta gccaaaatct tcctacaata attaatacaa ctacatttat taactgaaaa
     2281 aacaaacgac taaaaccaat accagtaaga atattaagtt tatttaccgg agatgcataa
     2341 aaccttttta atattaattg ctctcttaga ttgtaaaaag taaatgctat gccaaataag
     2401 gtagaaaata atatggaaaa acctatttga cctggtaaaa taaaatctat ttgccgatag
     2461 gctcttatgg aataaatatt tttttctatc tgaatatagt ttttaaaatc cgggttaatt
     2521 tttatagaaa tattttctaa aatgggtata agttgtgcaa cctctgcact tgtagcatta
     2581 gtagctgtta cattaattaa gtattggtgt ttattggcag tgtcgttact agcaatttta
     2641 ataatagctg ctaaatttcc tttttctaaa tctgcttttt gtaaagcctc attactataa
     2701 gtggctattt taatttgtgg taaatttttt aaaattgcaa atacagcatt attggtatct
     2761 gactggttac tgaaagcaat atttattttg ttaagcccac cattaccaaa cgatccaaaa
     2821 atccaaataa aaaccaacgg gaaaacaaga ctaaaaaaaa tggaacctgg attttttatg
     2881 atagccaata agcttgcctt tgtaatggct attaatgctg ttagctgact gtatttgtat
     2941 tgcatattat tattgaagtt gcaaacttat ttatattagt tagattattt ttattttttg
     3001 cagataaaaa caatctatta gcctaaaatt tatccctcaa atttttattt aattaaatgt
     3061 gttaaaacaa aagataaaat tatttttgta cattttatat aaacataatg gataaggcta
     3121 aaaaagaata tatagtttat ttgcttacag gtagcaatat tggcaataaa aaagaaaatt
     3181 tataccatgc taaacactta gttgataagc attgtggcaa cgtatctaat cactccccaa
     3241 tctaccaaac tgcagcttgg ggcttaacca accaaccaag cttttataac caagcattac
     3301 aattgcatac ttttttagaa cctgaaaact taatgcaaac attattaaat atagaagaaa
     3361 tgttaggcag agttaggaaa gaaaaaatgg gccccagaat aatagatatt gatattttgc
     3421 tgattgaaaa ctatactagc aacactaatt tactgcaagt accacatatt gcattgcctt
     3481 ttagaaaatt tgcattaata ccactggcag atattgctgc aacagtattg caccctatat
     3541 taaaaataac catagctgaa ttacttacta agtgtaagga tgatttagat gtgcaaaaga
     3601 tttaattatt atcatgcttt aggcttataa atttgaagcc ttgtatgaag catcatttta
     3661 ttactgttga aggaaatatt ggagctggta aaactaccct tactcattta cttgcaaaac
     3721 attttaatgc acgtattata ttagaagaat ttgcagacaa tcctttcttg tcaaaatttt
     3781 atgataaccc aaaacaatac gcttttccgg ttgaattatt ttttatggca gaaagatata
     3841 agcaaatgaa agatatggta catacgaaag atttatttca aacagtaaca gtatctgatt
     3901 atttgtttac aaaatgtttg ctttttgcca aagttacctt acccgaagag gagtttagat
     3961 tatatcaaaa gttgtttgat atcattcatc agcaactact ttttccagat atattaatat
     4021 acttacatgc acctgttcaa aaactacaag aaaatataaa aaaaagaaac agagagtatg
     4081 agcaacaaat taaagatgaa tatttatata atttgcaaga aacctataca agctatataa
     4141 agcagcataa tataaaaact atttttatag atgcctctaa tgcagatttt ttaggaaatg
     4201 aaaaacattt tcaggttgtt ttaaatgcat tagaaaaaga tattgacagt gggcaacatt
     4261 atttttcttt accttaaatc caaggtttat aaattattct atacaactga ataatggctg
     4321 ttataaaaaa cacaacaccc attatgaggt ttaaagtttt attactagtt ttcattttat
     4381 tgatagccca gttgccacca tgtatataaa caccaatacc tgctaaagta cctaacccag
     4441 ccccaataca aaagaaatta aactgatgaa agttggtttg taatacatta ttttgaataa
     4501 gttgagtgct ccataaaaac caaaatggta tttgaactgg gtttaaagca ctaatagtta
     4561 cacccagcaa aaatctatta attttattat ttattaaaag cccttttttt tctaccactt
     4621 gtttatatgc acttataaaa gcaagtacac ctaatattaa aaaaaccaca actgtaatcc
     4681 aacccaataa tttaaaaata tatttatact gagttaccaa actcatgcct gttaaagcca
     4741 atcttaaata aataatttca actattgcaa ctccaatacc aaatttccaa gccttattaa
     4801 agccttcctg cacacttaat tgtgtagctg ccatgttcat attacttaaa acaatttgcc
     4861 ccaaaaaact aattccaaat gctattgata aaataatcaa ataagtcata atagtttaaa
     4921 attagttatg aaatttttaa gataaatgaa ttagcaaatt ttttcgtatt cttctaaaac
     4981 agcataagct gtaaaggcgt cagctaaaaa ttttcttcct tgtttaaaag aaatataggg
     5041 tatgccttta tggtaattta ttctaccaat attatacata tctgcccaat gtgcagcaat
     5101 tgtttttaaa gctttaaagt aactcatgtt gcaatcataa atatgatttg gctctgcacc
     5161 ggtgccatta aactctaata tgctaatatt tttacctgct tttaaatctt ttagattagt
     5221 agttttaata tcaaatctac cgtagtaaaa atgtttagta taattactta tttcatcaaa
     5281 aacattggta agttgctgat ctatttcttg gtataaattt acaaaagtgg cacccctgtt
     5341 atgattgcct gtaatactta aaaaataagg ttcattattt tcaataatat cttgtaaatg
     5401 ttctgcatgt ttaacagtta attcatttaa cctaaatttt gctttagggt gttgttgaat
     5461 taactctaat aaagtttgtt taccattacc ttttacttgt aaatattctt ttgcaattaa
     5521 gccagtaatc ttgccttttg tgctgttagg gtaacgaata taaaacacac ttaattctaa
     5581 agggtattct ataaaagatt gtacaaggta ctcaaaaggc actgttgcat gatattgttt
     5641 tagctgctct tcattttcta tttttctaaa acataaaccc tttgtgccaa tatctggttt
     5701 tactgcaaca ggataatgta aagctgcttt atttacttgt tgaaccacct ccataaaatt
     5761 acaagctggc ttaatgtaaa tggttgttgg aaaagtgtgt aacggcaact gctcaaacat
     5821 ttctttttta gtttcgcctt ctaaacccga aaattgtaaa cttggattta cattagaaaa
     5881 ataccaaaac ttaccagttc ttataaaata atacaaccaa acaaaaacca atggagtata
     5941 aattacataa aaattccact gctcccaatc tgcaattttt tgcaaaattt ttttcaaatt
     6001 ctaattttta ttggcggcaa aaatagttag ttagcatgta ttaaaatact gttttttagt
     6061 aattaactac atagtttcct atattaataa tgcatttcta agtaattact cagggcatct
     6121 actaaatctg tattctcttt ttcatttcca acagttattc taatacagtt atggcaattg
     6181 ggtaaatgac tcacattttt aacagcaatc tgtacactta acaaataatc agttagctta
     6241 ctggcattat ctacttctat taaaataaaa ttggcatcgc tatcatgtac tttaataata
     6301 aaaggaattt gcttaaaaac atctgccaaa gcaattctca tttccaccaa aagttttatc
     6361 atatcgttca cctgcccaat ttcttcaaaa gcttttaagg ctgtttcttt acttactgtg
     6421 ctaatagtat tggtgttttt aactttgttt aaaatagtaa tagtattggg gttagataga
     6481 gcgatggcta accttaaacc agccaaaccc cacgcaatac taaaggtttg aagtataatt
     6541 aagttgtcat attcttttag taaagcagtt aaagactttt gtgcagaaaa attaatatag
     6601 gcttcatcta ctaccaccaa accattaaag ttatttaaaa caaattcaat atcgtttaag
     6661 tttaaactgt tgcctgttgg gttattgggc gaacagagcc aaataatttt ggtatgtgta
     6721 tctactaaat tttctaatgt aatcaaatcc aactgaaaat tttctagtag tactgctttt
     6781 cgtacttcaa tattataaat gtctgcaaaa ttttcgaatt gactataagt tggtggacaa
     6841 ataataacat tatccttttc aggggcacaa aagcatttgt ataaaatttc tatactttca
     6901 ttagtaccat tggctaaaaa aacattttcg gttgttacat tttttaaagc agcaatagct
     6961 tgttttaaat tattttgttc atcatcagga tatctattgt accatttttt taaagggcta
     7021 cctaagctgt tttcattttt atgtaaacta atttttattt cgtttttaga aatattattt
     7081 ttttggttta caattttttc aatgtttggt cttaaaagat ggttgatatt aaaagccata
     7141 actgctttgt atatgataat tttaaaataa aaggctaaat taaatgcttt aagcatactt
     7201 cgtacatttg cacaagttca aataaattat gaaacaattt caagtaccta ctaattatag
     7261 aagcagcctt ataagtgctg taaaaaataa acgaaaacaa gaagata
//
LOCUS       DAOQPK010000096         7278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_5760_length_7278_cov_4.378652, whole genome shotgun sequence.
ACCESSION   DAOQPK010000096 DAOQPK010000000
VERSION     DAOQPK010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7278
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5760_length_7278_cov_4.378652"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PLU36_05930"
     CDS             complement(<1..389)
                     /locus_tag="PLU36_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HMZ46325.1"
                     /translation="MAIIDIKLPNYITKALRLPENNPRRQQIKVLKKLLRKARFTQFG
                     QKYKFDEILMSKHAGKKFQEIVPVYNYNSIYKEWWHKTLEGKPDICWPGKIKYYALSS
                     GTSEAASKYIPVTNDLLNGNKIVMIKQ"
     gene            complement(489..2435)
                     /locus_tag="PLU36_05935"
     CDS             complement(489..2435)
                     /locus_tag="PLU36_05935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ46326.1"
                     /translation="MQLSIIIVNFNVKYFLQQCLYAVQKAINTIDAEVFVVDNNSTDG
                     SVAYLQPFFPWVKFIQNKENIGFGKANNIAFAQAKGEFILFLNPDTIVPEDCFTKCIA
                     FINKQTNCAALGIKMLDGQGHFLPESKRSFPSPITSFYKLIGASYLFPTSKHFNKYAL
                     GYLDKNKNHEVDVLAGAFIMTRKELLDKIGVFDEKFFMYGEDVDLSYRLQQTGLKNYY
                     FADSCIIHFKGESTKRGSLNYVKMFYEAMSIFVSKHYKGSYATIFSSTIKIAIWIRAV
                     LSLLKRLFTKISLAIIDSLVIYFCIWLVKTAWVTYIRHGNEFLQNIVNTITPIYVLVF
                     LTAAAFAGMYDNLYKPVKAIAASLVAIVAGLAVYSLLPENVRFSRGVILFGGITSGFM
                     LIFYRWVLTKMGLIKETDEENKMQQTVIVGTIEEYTNAFNLLKTVNKQERVLGRIDVL
                     NEPNGNSIGSFNQLQKIIETLKIKEIIFCGGTLSYQQIINQVQQLSGKINFRFYENES
                     SCIIGSDSKTATGEILNTEVVYSITNPYQARMKRVVDIFTALFILVTFPFQLLFIKKT
                     ANAILNALKVLIGKNTWVGYANLTPTLPVLKPAIITHYGIATKKKQFANTNVLEKLDF
                     MYAKDYHWLQDVKSIINSYKKLGG"
     gene            complement(2520..4016)
                     /locus_tag="PLU36_05940"
     CDS             complement(2520..4016)
                     /locus_tag="PLU36_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46327.1"
                     /translation="MLSDKEIKDKLNNYSSPVSERVWQNIVVEQQKNKSIIQNYFTGA
                     RLSFFVGLLLTTVTLGYIIKQQSNRSVNLANSSGLLTQKTNQNITQNIFFNNYSKQNK
                     ELEQKVAFGESKLNSPLTSSNGVSKIKIDKNATVKNTNQLIPNNKFYTSSSVNTNVYT
                     ATESINNNTTDNYKSGNDSYHSTVDNISNSNLHSEELNIQENNQSNLIKGTTEKVFLL
                     TTLPLAKIANQKLSYKLYQNTEPLECPGEYSSYKKNWYVEGFIAPEYVMKKVYSNNPA
                     NDAYLSKKDSVEKMVAGISIGAKFTRGLSKNLYIKGGLQYNQMIEKTSLMMEKERKEI
                     TVITINTVTDINGNTVTVADTSKQLQITYARSVNYNSIKSIELPISLGYEFYRKNFRA
                     SVNAGFIVNLSSWYSGKIFDTSYQMVNIKERPGIYKQNVGLSLYGSISLIKPLSESAE
                     IFAEPYFRYSLTNPKYNAYGFKQKFNAVGLSMGLRFKLNKNYSSRTFN"
     gene            complement(4027..4542)
                     /locus_tag="PLU36_05945"
     CDS             complement(4027..4542)
                     /locus_tag="PLU36_05945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ46328.1"
                     /translation="MIKGCIRQDATCQRLLYEQLSGKMFTVCLRYANDLMEAEDLLQE
                     GFIRVFSNVHQFKFEGSFEGWVRRIMVNSALKCIQKKKIIFSELKESAAATYHYYTYS
                     NLGEEELLKLISQLPHGYKVVFNLYVIDGYSHDEIGQMLQIQPSTSRSQLVKARKMLQ
                     QQILQLEKIAV"
     gene            complement(4696..5187)
                     /gene="rfaE2"
                     /locus_tag="PLU36_05950"
     CDS             complement(4696..5187)
                     /gene="rfaE2"
                     /locus_tag="PLU36_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HMZ46329.1"
                     /translation="MKQIAAIEKKIFDLPALVKQVAIWKKFNKTIAFTNGCFDILHDG
                     HIYSLSQAAKEADYLIVAVNSDSSIKKIKGEDRPINHQQSRAMMLASFAIIDAVIIFE
                     EDTPYNLITTLLPNVLVKGGDYTINQIIGAEEVIKNGGNVVINPIIQGFSTTGIINKI
                     KNL"
     gene            5309..6220
                     /locus_tag="PLU36_05955"
     CDS             5309..6220
                     /locus_tag="PLU36_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HMZ46330.1"
                     /translation="MIKVPSYLKKGDTIGLVCPAGYMPLNKATTCINTLQNWGYNVCI
                     GKTVGGKQKNYFSGTDVERLNDFQAMLDDDAIKAILCARGGYGVSRIIDQLNFTKFKK
                     NPKWIIGFSDITVFHAHINNQYNIATMHAPMAAAFNDNGFKNEYILSLKNALAGKKNK
                     YYCKPHILNNLGKINGELIGGNLALITHLIGSKSAYNTNKKILFIEDIGEYLYNIDRM
                     FVQLKRSGALNNLNALIIGGFSELKDTSKPFGQDVYNIIHFHVKDYNYPICFNFPVSH
                     NRENVTLKIGINYELNIQKNSVVLKEL"
     gene            complement(6226..>7278)
                     /locus_tag="PLU36_05960"
     CDS             complement(6226..>7278)
                     /locus_tag="PLU36_05960"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HMZ46331.1"
                     /translation="ITDNLEKAKKIYNYVQSNIKVNYTSNIDISFPIKKIFQNKKGNA
                     IQVNALLATLLSSADLPVKPILLSTRENGKPSELYPIIDEFNYMVCYLEINDQKYFLD
                     ASSNKLGFGKLPLKCYNGAARLIDTTMPSIINISADSLLETTKTNVYIINKEEGLGMT
                     GSFTSSLGYSKSTEIRERLLTNNESDIFNEQKNKFEFKTDITNSVIENLNDLETSLNL
                     KYNFSLDSEAETINFLPLLTEAIKENPFKETKRNYPIEMPYTISQTYLFSMETPKGYE
                     IAEMPKSARILLNETEGLFEYLIAKKSNGSIQLKCVVKINVANFNAAEDFETLKGFYN
                     FIIQKESEPIVFKKKK"
BASE COUNT         2408 a         1110 c          946 g         2814 t
ORIGIN      
        1 aattgtttta tcattacaat cttattgcca tttaaaaggt cgttggttac tggtatgtat
       61 ttacttgctg cttcgctggt gccagatgaa agtgcataat attttatttt tcctggccag
      121 caaatatctg gtttaccttc taaggtttta tgccaccact ctttataaat ggagttgtaa
      181 ttatacacag gtacaatttc ctgaaatttt ttaccagcat gtttgctcat taaaatttca
      241 tcaaacttat atttttgtcc aaactgtgta aatcgggctt tgcgtaataa tttttttaaa
      301 actttaattt gttgcctacg aggattattc tctggcaatc ttaaagcttt ggtaatataa
      361 ttaggcagtt taatatcaat tattgccatc ttttaataaa gctggtttta atagtttcaa
      421 aacaatcgtt agttactcaa caaatgtaac atattgtttt attcaaatat caacctacaa
      481 caaaaacatt atcctcctaa ttttttatac gaattgatta ttgattttac atcttgcagc
      541 caatgataat ctttagcata catgaaatct aatttctcta aaacattggt attagcaaac
      601 tgttttttct ttgttgctat tccgtaatga gtaataattg ctggttttag aactggcaaa
      661 gtaggtgtta aatttgcata cccaacccaa gtatttttac ctattaatac ttttagtgca
      721 tttaaaattg cattagcagt ttttttgata aaaagtaatt gaaaaggaaa agtaactaaa
      781 ataaaaaggg cagtaaaaat atctaccact cttttcattc ttgcctgata aggattagta
      841 atagaatata caacttctgt atttaaaatt tcaccagttg ccgttttaga atcactgcct
      901 ataatacaac tactttcatt ttcataaaat ctaaaattaa ttttaccaga taattgctgc
      961 acttgattaa ttatttgctg gtaagataat gttccgccac aaaaaataat ttctttaatt
     1021 tttaatgttt ctataatttt ttgtagctga ttaaagctac caattgaatt gccatttggt
     1081 tcgttcaaaa catctattct tcctaaaact ctttcttgct tatttacagt tttaagtaaa
     1141 ttaaatgcat tcgtgtactc ttcaattgtg cctacaataa ccgtttgttg cattttattt
     1201 tcttcatctg tttctttaat caatcccatt ttagttaata cccatctgta aaaaataagc
     1261 ataaagccag aggtaatgcc accaaataat attactcctc tagaaaatct aacattttct
     1321 ggcaaaagtg aatataccgc taatcctgca acaattgcta ctaacgaagc tgcaatggct
     1381 ttaacaggct tatacaaatt atcgtacata cctgcaaatg ctgcagcagt taaaaaaact
     1441 aaaacataaa ttggcgtaat tgtgtttaca atattttgta aaaactcgtt accatgcctt
     1501 atataagtaa cccatgctgt ttttactaac catatacaaa aataaattac caaactatca
     1561 attatagcta aactaatttt ggtaaacaac ctttttaata aagataaaac tgctcttatc
     1621 caaatggcaa ttttaatagt tgaagaaaaa atggtagcat agctgccttt ataatgtttg
     1681 cttacaaaaa tgctcattgc ttcgtaaaac atttttacat aatttaggct gccacgtttg
     1741 gtactttctc ctttaaaatg aataatgcaa ctatctgcaa aataataatt tttaagtcca
     1801 gtttgttgta acctataact taaatctaca tcttcgccat acataaaaaa cttctcatca
     1861 aaaacaccaa tcttatctaa taactccttt cttgtcatta taaaagcacc agctaaaaca
     1921 tccacttcat gatttttatt tttatctaaa tatcctaaag cgtatttatt aaaatgctta
     1981 gaagttggaa ataaatacga agcccctatg agcttataga atgatgtaat tggagacgga
     2041 aaagatcttt tactttctgg taaaaaatgg ccctgcccat ctaacatttt aattcctagg
     2101 gctgcacaat ttgtttgctt atttataaag gcaatgcatt ttgtaaaaca atcttctggt
     2161 actatagtat ctggatttag aaataaaata aattctcctt tagcttgtgc aaaagcaata
     2221 ttatttgcct ttccaaatcc tatattttct ttattttgta taaatttcac ccatgggaaa
     2281 aagggttgta aataggcaac actaccatct gttgaattat tatccaccac aaaaacttct
     2341 gcatcaatag tattaatagc tttttgtaca gcgtacaaac actgttgaag aaaatatttt
     2401 acattaaaat ttactattat tattgatagc tgcacctatt tatacaaact tatttgcagg
     2461 caacatactt tgtatttacg aagttgccaa aacttaataa aatttttgag aaaataaatt
     2521 tagttaaaag ttctgcttga gtaattttta tttaatttaa atctcaaccc cattgataat
     2581 ccaacagcat taaatttttg tttaaaccca taggcattat actttgggtt tgttaaactg
     2641 taacgaaaat agggttctgc aaatatttca gcagattcgg ataatggctt tattaaactg
     2701 atgctaccat acaaacttaa gcctacattt tgtttataaa ttccagggcg ttcttttata
     2761 tttaccattt gataacttgt atcaaatatt ttaccactat accaactaga taggtttaca
     2821 ataaagccag cattaacaga tgctctgaaa ttttttctat aaaattcgta ccctaaagaa
     2881 attggcaact ctatactttt tattgaattg taattaacac tacgggcata agttatttgt
     2941 aattgtttag aagtatctgc aactgttaca gtatttccgt taatatcagt aactgtatta
     3001 atggttatta cagtaatctc ctttctttct ttttccatca tcaggcttgt tttttcaatc
     3061 atttgattgt attgtaaacc gcctttaatg tataaatttt tagataatcc tcttgtaaat
     3121 tttgcaccta tgctaatacc tgcaaccatt ttttctacac tatctttctt gcttaaataa
     3181 gcatcatttg ccgggttatt actatatact tttttcatca catactctgg tgcaataaac
     3241 ccttcaacat accaattctt tttataacta ctatattctc ctgggcattc taatggttct
     3301 gtattttgat atagtttata agatagtttt tggtttgcaa ttttggctaa tggaagtgta
     3361 gtaagtaaga atactttttc agtagtgcct tttattaaat tactttgatt attttcttga
     3421 atatttaatt cttctgaatg aagattacta ttggatatat tatctactgt tgaatgataa
     3481 ctatcattac ctgatttgta attatcagtt gtgttattat ttatactttc cgttgcagta
     3541 taaacattag tatttacact gctacttgta taaaatttat tatttggtat taattgatta
     3601 gtatttttaa cagtagcgtt tttatctatt ttaattttgc taactccatt tgatgaagtt
     3661 agtggagaat ttagttttga ttcaccaaat gcaacttttt gttctaactc tttattttgt
     3721 ttactgtaat tattaaaaaa aatattttga gtaatgtttt gattagtttt ttgtgttagt
     3781 aagccgctac tattagctaa atttactgag cgattgcttt gctgtttaat aatataacct
     3841 agtgttacag tggtaagtaa taacccaaca aagaaagaca atcttgcacc agtaaaataa
     3901 ttttgaataa tagatttatt cttttgttgt tctacaacaa tattttgcca taccctttct
     3961 gatacaggag atgaataatt atttaactta tcttttattt ctttatcaga caacataatc
     4021 tattacctat actgcaattt tttctaattg taaaatttgt tgttgcaaca ttttcctagc
     4081 ttttactaat tggcttctgc ttgtactagg ttgtatttgt aacatctgcc caatctcatc
     4141 atgagaatat ccatcaatta cataaaggtt aaaaacaacc ttataaccat gtggcaattg
     4201 gctaattagc ttcaataatt cttcttcgcc taaattggag taagtataat aatgataagt
     4261 tgcagcagca gactctttta attcgctaaa aataattttc tttttttgaa tacattttaa
     4321 tgcactattc accattattc ttcttaccca accttcaaat gaaccttcaa acttaaactg
     4381 gtgtacgttg ctaaatactc ttataaaacc ttcttgtaac aaatcttcgg cctccattaa
     4441 atcgttggca tatcgtaaac aaactgtaaa cattttgcca ctcaattgtt catataacaa
     4501 tcgttgacaa gtggcatctt gcctaataca cccttttatc atttcaattt cagtcaaagt
     4561 agtgtgttaa ttttttatgt ttacttaaat agtgatactg cagtaattgt ttatgctgct
     4621 ttgaatgcat aaagttaatt tttttttatt actctaccac taataaatta tttagttgat
     4681 tattaagaac ttatcttata aatttttaat tttatttata attccggttg ttgaaaaacc
     4741 ttgaatgatg ggatttataa caacattccc tccatttttt attacttctt cggcacctat
     4801 aatttggttt attgtataat ctccgccttt tactaataca ttaggtaata aggttgtaat
     4861 taaattatat ggtgtatctt cttcaaaaat tattacagca tcaataatgg caaaacttgc
     4921 taacatcatt gctctacttt gctgatggtt tattggtcta tcttctccct taattttttt
     4981 tatactacta tcgctattaa ctgcaactat taaataatct gcttctttag cagcctgcga
     5041 taaagagtat atatgtccat cgtgtaaaat atcaaaacaa ccattagtaa aagcaatagt
     5101 tttattaaat tttttccaaa tggctacctg tttaaccaat gctggtaaat cgaatatttt
     5161 tttttctatt gctgctattt gtttcattta atgtatttat tgaaatgtat attgcaaggt
     5221 aaataaaatt accaacctat tttagagaaa attttgtgtt taaagtatat taaatacaaa
     5281 tattgagtat ggtttgtaat attgctatat gattaaagtt ccttcgtatc ttaaaaaagg
     5341 cgatacaata ggtttagttt gcccagcagg ctatatgcct ttaaataaag caaccacttg
     5401 cattaatact ttacaaaact gggggtataa tgtttgtatt ggaaaaactg ttggtggtaa
     5461 acaaaaaaat tatttttctg gaacagatgt agaaaggtta aatgactttc aggcaatgtt
     5521 agatgatgat gctattaaag ccattctttg tgcaagaggt ggttatggag taagcagaat
     5581 tattgaccaa ttaaatttta caaaatttaa aaaaaatcct aaatggatta ttggatttag
     5641 cgatataaca gtttttcatg cacacataaa caaccaatat aatattgcaa ctatgcatgc
     5701 acctatggct gctgcattta atgataatgg atttaagaat gaatatattc tatcattaaa
     5761 aaatgcttta gcaggtaaaa agaataaata ctattgcaag cctcatatat taaataattt
     5821 aggtaaaata aatggtgaat taattggcgg aaatttggca ttaataacac atttaattgg
     5881 ctccaagtct gcatataaca ccaataaaaa aatattattt attgaagata ttggtgaata
     5941 tttatataat atagatagaa tgtttgtaca attaaaacgc agtggtgcat taaataactt
     6001 aaatgcttta ataattggcg gattttctga actaaaagat acttctaagc catttggaca
     6061 ggatgtttac aatatcattc attttcatgt aaaagactat aactacccaa tttgttttaa
     6121 ttttcctgta agccataata gagaaaatgt aacgttgaaa attggtataa actacgaatt
     6181 aaacatacaa aaaaacagcg tagttttaaa agaactttag tggttttatt ttttcttttt
     6241 aaacacaatt ggttcgcttt ctttttgaat aataaaattg taaaaccctt ttaaggtttc
     6301 aaaatcttct gctgcattaa aattggcaac attaattttt acaacacatt taagttgtat
     6361 tgacccattg gattttttgg caattaaata ttcaaacaat ccttcggttt cattaagtaa
     6421 tattcttgca ctttttggca tttctgcaat ttcatagcct ttaggagttt ccatagaaaa
     6481 taaataagtt tgacttatag tatagggcat ttctattgga taatttcttt tagtttcttt
     6541 aaacggattt tctttaattg cttctgtaag caagggtaaa aaattaattg tttcggcctc
     6601 tgaatctaaa ctaaaattgt actttaaatt taacgatgtc tctaagtcat ttaaattttc
     6661 aataacagaa ttggtaatat ctgttttaaa ttcaaattta tttttttgtt cattaaaaat
     6721 atcagattca ttattcgtca atagtctttc tctaatttct gtagattttg aataacccaa
     6781 tgatgaagta aaggaaccag tcattccaag cccttcttct ttattgataa tatatacatt
     6841 tgttttagta gtttcaagca acgagtctgc agaaatattt ataatagatg gcatagttgt
     6901 atcaatcaat cttgcagctc cgttataaca ttttaatggt aattttccga accctaattt
     6961 atttgaagaa gcatctaaaa aatatttttg atcgttaatt tctaaatagc aaaccatata
     7021 attaaactca tcaataattg gatacagctc agatggttta ccattttctc ttgtgcttag
     7081 caatataggc ttaacaggta aatctgctga actgagcaag gttgctaaca aagcatttac
     7141 ctgtattgca tttccttttt tattctgaaa tatttttttt attggaaatg aaatatcaat
     7201 atttgaagtg taatttactt ttatatttga ttgaacatag ttgtaaattt tttttgcttt
     7261 ttctaaatta tctgttat
//
LOCUS       DAOQPK010000097         7167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_5881_length_7167_cov_3.683211, whole genome shotgun sequence.
ACCESSION   DAOQPK010000097 DAOQPK010000000
VERSION     DAOQPK010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7167
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5881_length_7167_cov_3.683211"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /gene="atpD"
                     /locus_tag="PLU36_05965"
     CDS             <1..1081
                     /gene="atpD"
                     /locus_tag="PLU36_05965"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HMZ46332.1"
                     /translation="FAKGGKVGLFGGAGVGKTVLIQELINNIAKGHGGLSVFAGVGER
                     TREGNDLLREMIEAGIMKYGDGFKHSMEEGGWDLSKVDLEGLKESKATFVFGQMNEPP
                     GARARVALSGLTIAEYFRDGDGSGKGKDILFFVDNIFRFTQAGSEVSALLGRMPSAVG
                     YQPTLATEMGLMQERITSTKHGSITSVQAVYVPADDLTDPAPATTFAHLDATTVLSRK
                     IADLGIYPAVDPLDSTSRILTPAVVGEAHYNTANRVKLILQRYKELQDIIAILGMDEL
                     SDDDKMTVSRARKVQRFLSQPFHVAEQFTGLKGVFVNIDDTIRGFNAIMDGEVDEYPE
                     AAFNLVGTLEDAIEKGKKMMAQAHG"
     gene            1123..1374
                     /gene="atpC"
                     /locus_tag="PLU36_05970"
     CDS             1123..1374
                     /gene="atpC"
                     /locus_tag="PLU36_05970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HMZ46333.1"
                     /translation="MTLEILTPEKKLYSGDVYGVQLPGINGLFEVLDKHAPLVSALNK
                     GKIKILKSKTSFEYYTIQKGFVEVLNNKATVLVEGAVEA"
     gene            1452..1991
                     /locus_tag="PLU36_05975"
     CDS             1452..1991
                     /locus_tag="PLU36_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46334.1"
                     /translation="MQQLGIGSRVEHTHFGKGVIVDIGSEFYTIWFKLQNTTKSIGKD
                     FELKVIEATENTQQLNNISLADIELALNNILEQRLHEFQLVPMANKWQNGNLILQPAD
                     ATLQPKEIPIETFFHKIVMVRDRLRLIEQKINANKVLTDEEKIDLQQYITAIYGSLTT
                     FNVLFKEAHHQFKGMSTKD"
     gene            2179..2478
                     /locus_tag="PLU36_05980"
     CDS             2179..2478
                     /locus_tag="PLU36_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HMZ46335.1"
                     /translation="MNIKIQAVRFNADQKLIDLVARKVEKLKTYHDKIIQVDVFLKLD
                     NVVHNIKDKIAEIRVTIPRHEFFVKASSKSFEESFENALLSISNQIKRKKEKLAA"
     gene            2590..3591
                     /gene="holA"
                     /locus_tag="PLU36_05985"
     CDS             2590..3591
                     /gene="holA"
                     /locus_tag="PLU36_05985"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HMZ46336.1"
                     /translation="MSADKIISSWMKGKYAPVYFLDGEEPYFIDKIINYAETKIINEN
                     ETAFNLSIFYGRDSNWADIVNACKRYPMFADKQVVIIKEAQHLKEIDKLENYIIHPLP
                     STILVIAYKDKKLDARTKFAKTIKEKAEIFTSKKLNDNEVINWTSKIIEDYNLKISQS
                     ALHLLVEHIGNNLVRIANEIEKLSITLRNDKTITEDAIELYIGISKDYNEFELQKVIG
                     RKDFTKAMRIVQYYEKNPTQTPIQLILISLCNYFSKVYALYTIGNNNENTIATTLGIH
                     PFYVKDYIYSTRIYQTQEVEKILLLLNKYALKSVGVDNASSNQAVLLKELTIKIFSN"
     gene            complement(3596..4489)
                     /locus_tag="PLU36_05990"
     CDS             complement(3596..4489)
                     /locus_tag="PLU36_05990"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HMZ46337.1"
                     /translation="MNCPKGKLIAIGGAEDKGTDLEKGEILRNNLNFFELGILKRIIE
                     ETGGLDKRIEVVTTASSIPYEVGENYLNAFGKIGCTNVGLMHIRNREDVKKEEYLERV
                     KQCNAIMFTGGNQMRLSSIFGGTTFLDIIKSRYLQEEFIIAGTSAGAMAMSNTMIYEG
                     NATKAHLKGEVKITTGLAFMENVIFDSHFEKRGRFGRLAQAVATNPSCIGIGLGEDTG
                     MMITKGNVMQAIGSGLVIIIDGHELGHCNIADIPEGNPISVENLKVHFCENGNGYLVN
                     ERKFIVEVAQGALVEKKVEVE"
     gene            4688..>5238
                     /locus_tag="PLU36_05995"
     CDS             4688..>5238
                     /locus_tag="PLU36_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ46338.1"
                     /translation="MQKLLFTLLFWQLHVTTVFAKADTSIVVFFDFNKYELTQNSTNI
                     LKQFCADTLVLELIEIYGHTDQIGKEKYNLLLSQKRANSVKQFLLTNFIDNNKIIVTQ
                     GKGEKELITNKTKEEFRKENRRVVIYFEKRQPIIKPAIVKKKPEEKPQKPIEKETTLT
                     EKIKDSTVKTGDNLILKNINFVGG"
     assembly_gap    5239..5338
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5557..>7167
                     /locus_tag="PLU36_06000"
     CDS             5557..>7167
                     /locus_tag="PLU36_06000"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HMZ46339.1"
                     /translation="MQVKQFCFLVSILFCCNHIIAQPNRWQQKIKYTITVELNDTENK
                     LNGIEKIEYTNNSLDTLNKIFFHLYWNAFQPNSSMDVRSRELGKTVIRKDKDGKDVLD
                     WDGRVTNRISQLKENEIGIQKVKYVVINGKQQKLIEHETILEVVLDKPILPNSKIIMN
                     VSFEAQVPIQIRRAGRDNAEGIKFSMSQWYPKVVEYDYNGWNANPYIAREFYGVWGDY
                     DVKITLNNKYTVAATGVLQNPNTIGKGYSTPGLKVAKPSKDVITWNFIANNVHDFVWA
                     ADPDYTHESKEIRKGLTLHLFYKSTTPANDSAWHNILWAAEKVMPYIEKRFGKYPYPQ
                     YSFIQGGDGGMEYAMATLIKGSGLGTVFHEWMHSWYQFVLANNESLYGWMDEGFTTFA
                     EDEVTEWYNKNYAWQSPWITEKAKQKIIFQNKNNDTELPLKQYNNYAAYFNLVKSPFE
                     EPLSTHADHFNTNFGYSYASYIKGSVFLTQLGYITSDSVRDKILLAYYDTWKFKHPNV
                     NDFIRVAEKVSGLELQWYKEYWVYTTKNI"
BASE COUNT         2653 a         1054 c         1119 g         2241 t
ORIGIN      
        1 ttttgctaag ggtggtaaag ttggtttatt tggtggtgct ggtgtaggta aaacagtatt
       61 aattcaagag ttgattaata atattgctaa aggccatggt ggtttatcgg tatttgcagg
      121 tgtgggcgaa agaacacgtg aaggaaatga tttattaaga gaaatgattg aagccggcat
      181 tatgaaatat ggcgatggct ttaaacatag tatggaagaa ggtggctggg atttaagtaa
      241 agtagattta gaaggcttaa aagaaagtaa agcaactttt gtatttggac aaatgaacga
      301 gcctcctgga gcaagagcaa gagtggcttt aagtggttta actattgctg aatattttcg
      361 tgatggagat ggctctggca aaggaaaaga cattctattt ttcgtagata atatcttccg
      421 ttttacacaa gctggttcag aagtttcggc tctattaggt cgtatgcctt ctgcagtagg
      481 ttatcagcca actttagcaa cagaaatggg cttaatgcaa gaaagaatta cttctactaa
      541 acatggctca attacatctg tacaagctgt ttatgtgcct gcagatgact taactgaccc
      601 tgctcctgct actacctttg ctcacttaga tgctactaca gtattaagta gaaaaattgc
      661 cgacttagga atctatccag cagttgaccc attagactct acctctagaa ttttaacccc
      721 tgctgtagta ggagaagccc actacaacac tgctaataga gtaaaattaa tattacaacg
      781 ctataaagag ctacaagata ttattgctat tttgggtatg gatgaattga gtgatgatga
      841 taaaatgact gtttctcgtg caagaaaggt acaacgtttc ttatctcaac catttcatgt
      901 ggcagaacaa tttacaggct taaaaggtgt atttgtaaat attgacgata ccattagagg
      961 ctttaatgca attatggatg gcgaagtgga tgaatatcca gaagctgcat ttaaccttgt
     1021 aggtacttta gaagatgcca ttgaaaaagg taaaaaaatg atggctcagg ctcacggata
     1081 attggtattt taatgattaa attttaaagt aaatacaaaa atatgacttt agaaatatta
     1141 acaccagaaa aaaagcttta tagtggagat gtgtatgggg tacaattacc tggtattaat
     1201 ggtttatttg aagtgttaga taaacatgca cctttagtta gtgccttaaa taaaggaaaa
     1261 attaaaatct taaaatctaa aacaagtttt gaatactata ctattcaaaa aggctttgta
     1321 gaggttttaa ataataaagc aactgtactg gtagaaggtg ctgtagaagc ataaaataat
     1381 ataacttatc ttatccttaa aatctctctg cactaagaga gattttttta ttttctttac
     1441 aacaaaaaat tatgcaacaa ttaggtattg gatcaagagt agaacataca cattttggca
     1501 aaggtgttat tgtagatatt ggatctgagt tttatacaat ttggtttaaa ttacaaaata
     1561 ccaccaaaag cataggcaaa gattttgaat taaaagttat agaagcaact gaaaatactc
     1621 aacaattaaa caatatttca ttagccgata ttgaacttgc tttaaacaat attttagaac
     1681 aacgtttaca cgaatttcag ttagtgccaa tggcaaataa atggcaaaac ggaaatttaa
     1741 ttctacaacc tgccgatgct accttacaac caaaagaaat accaatagaa accttctttc
     1801 ataaaatagt aatggtaaga gataggctaa gactgataga acaaaagatt aatgcaaata
     1861 aagtattaac cgatgaagaa aaaattgatt tacaacaata tattactgca atatatggtt
     1921 ccttaaccac gtttaatgtt ttgtttaaag aagcccatca tcagtttaaa ggcatgagta
     1981 caaaagacta ataaatacct attaaaggta aattattgca agccgtgaat tgaaaattta
     2041 ttatttacga aattttaaac attttttagt tttttattca ggttaaaaat gctaacttgt
     2101 aaccaataaa aataacgatt gcctatgtaa gttttctacc cttaaacatt tttattcacc
     2161 tttaaaacat tatttgatat gaacattaaa attcaagcag ttcgtttcaa tgcagatcag
     2221 aagttgattg acttagtagc tcgtaaggta gaaaaactta aaacctatca tgataaaatt
     2281 attcaggtag atgttttctt aaaactagat aatgtagtac ataatattaa agataaaatt
     2341 gcagaaataa gagtaaccat tccacgccat gaattttttg tgaaagcatc ttcaaaatca
     2401 ttcgaagaaa gttttgaaaa tgcactctta tctatctcta atcaaattaa acgtaaaaaa
     2461 gaaaaactgg cagcatagcc tgttgtaaaa gcctgcttta tttggcaggc ttttttattt
     2521 ttatcaacag tgtgctatta attaatataa tttgttcttc gcctactttg cactccttat
     2581 ttttgttaca tgtctgccga taaaattatt agctcttgga tgaaggggaa atatgcacct
     2641 gtttattttt tagatgggga ggaaccctat tttattgata aaataattaa ctatgccgaa
     2701 actaaaatta tcaatgaaaa tgaaacagct tttaacctta gtatttttta tggaagagat
     2761 tccaactggg cagatatagt gaatgcctgc aaacggtacc caatgtttgc agataaacaa
     2821 gttgtgataa ttaaagaagc acagcattta aaagaaatag ataaattaga aaactacatt
     2881 atccatcctt tgccatcaac cattttagta attgcttata aagataaaaa attagatgcc
     2941 cgaactaagt ttgcaaaaac aattaaagaa aaagccgaaa tttttacaag taaaaaactt
     3001 aatgataatg aagtaataaa ctggaccagc aaaattattg aagattataa tttaaaaatt
     3061 tcgcaaagtg cattgcattt attggtagag catattggaa ataacttagt acgcattgct
     3121 aatgaaatag aaaagttatc cattacttta agaaatgata aaaccattac agaagatgca
     3181 attgaactgt atattggaat aagtaaagat tataacgaat ttgaattaca aaaagtaatt
     3241 ggcagaaaag attttaccaa ggcaatgcgt attgtacagt actacgagaa aaaccctact
     3301 caaacaccca tacaattaat attaataagc ctttgtaatt attttagtaa agtgtatgca
     3361 ctatatacta ttggcaataa caacgaaaat actattgcca ctacccttgg cattcatcct
     3421 ttttatgtta aagactatat ttattctacc cgtatatacc aaacgcagga agtagagaaa
     3481 attttattgt tactaaataa atatgcatta aaaagtgtag gtgtagataa tgcaagtagt
     3541 aaccaagcag ttttattaaa agagttaact ataaaaatat tttcaaatta aatagttact
     3601 ccacttcaac ctttttttca actagtgctc cttgggcaac ttctactata aattttcttt
     3661 catttactaa ataaccatta ccgttttcac aaaaatgtac ttttaaattt tctacactta
     3721 ttgggttacc ttctggaata tctgcaatat tacaatggcc aagttcgtgt ccatcaataa
     3781 taataactaa accactacct atagcctgca ttacattacc ttttgtaatc atcatacctg
     3841 tatcttcacc taagccaata ccaatacaac ttgggttagt agctacagcc tgagccaatc
     3901 ttccaaatct tcctcttttt tcaaaatgag aatcaaaaat tacattttcc ataaatgcca
     3961 accctgtggt aatcttcact tcgcctttta aatgtgcttt ggtagcattc ccttcatata
     4021 tcattgtatt gctcattgcc atagcacctg cactagtacc agcaataata aattcttctt
     4081 gcagatatcg gcttttaata atatctaaaa atgtagttcc accaaaaata gaagacaacc
     4141 tcatttgatt accacctgta aacattattg cattgcattg tttaactctt tccaaatact
     4201 cttctttttt tacatcttct ctatttctta tatgcattaa cccaacatta gtacagccaa
     4261 tttttccaaa tgcattcaaa tagttttcgc ccacctcgta aggtatagaa gatgcagtgg
     4321 taacaacttc tattctttta tctaaaccac ctgtttcttc aattattctt tttaaaattc
     4381 ctaattcaaa aaaattaaga ttattacgta gtatttcacc tttttccaaa tcagtgcctt
     4441 tatcctctgc tccaccaatg gcaattaatt ttcctttagg acagttcatt caaaaaaaat
     4501 tttagttaat agggttctaa tgatttatct ggtttttgta taaatcaatt tttattcatt
     4561 aacaaatata tagtttacag cataatatta tgtgtttaat cttttacaca ttgtgtatat
     4621 tgtaattaca atatattcaa ataagtgtag ttttattggt atatcttgct attaaaacaa
     4681 tttttaaatg caaaaattac tctttacatt acttttttgg caactgcatg ttactacagt
     4741 atttgcaaaa gcagatacta gtattgtggt atttttcgat tttaacaagt atgaattaac
     4801 ccaaaattct acaaacattt taaaacaatt ttgtgccgat acattagttt tagaattgat
     4861 agaaatttat gggcataccg accaaattgg taaggaaaaa tataatttat tattatctca
     4921 aaagagagcc aatagtgtaa aacaattttt acttacaaac tttatagata acaataaaat
     4981 aattgttaca caaggcaaag gagaaaaaga attaattacc aataaaacca aagaagaatt
     5041 tagaaaagaa aacagaagag ttgtaattta ttttgaaaaa aggcagccca taattaagcc
     5101 tgcaatagtg aagaaaaagc ctgaagaaaa accccaaaaa ccaatagaaa aagaaaccac
     5161 tttaactgaa aaaataaaag acagtactgt aaaaacaggc gataacttaa ttttaaaaaa
     5221 catcaatttt gtaggtggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta
     5341 taaaggcttt gccagaaaat ttcctattac agatgaaagc aatgagtttg aaagaatgac
     5401 caacagaagg gtagaaataa aaattattaa aaaatagcct ctatcttatt cttaagccat
     5461 tttcttacca caattatata agttttaaga aaatattgta gcaatatttg tttctttggc
     5521 attgtacatg acatggaagt tttaaattta ttttttatgc aagtaaagca gttttgtttt
     5581 ttagtaagta ttcttttttg ttgtaatcat attattgctc aacccaatcg ttggcaacaa
     5641 aaaataaaat atactattac tgtagagttg aacgatacag aaaataaact aaatggtatt
     5701 gaaaaaattg aatataccaa caactcactc gacacattaa acaaaatatt ttttcattta
     5761 tactggaatg cttttcaacc taacagtagt atggatgtaa gaagcagaga actaggtaaa
     5821 acagttatta gaaaagataa agatggaaaa gatgtgttag actgggatgg tagagtaacc
     5881 aatagaatta gccagttaaa agaaaatgaa ataggaattc aaaaagtaaa atatgttgta
     5941 attaacggaa aacaacaaaa acttatagaa catgaaacaa ttttagaagt agtattagat
     6001 aaacctattt tacccaactc taaaataata atgaatgtaa gctttgaagc acaagtgccc
     6061 atacaaataa gaagagcagg aagagataat gcagaaggaa taaaatttag catgagccaa
     6121 tggtatccta aagttgtaga atacgattat aatggctgga atgccaaccc ttatattgcc
     6181 agagagtttt atggcgtttg gggagattat gatgtaaaaa ttacattaaa taataaatat
     6241 acagttgcag ctacaggtgt actacaaaat ccaaatacaa ttggtaaagg ctatagtaca
     6301 ccaggtttaa aagttgctaa accaagtaaa gatgtaatta catggaattt tattgcaaat
     6361 aatgttcatg attttgtttg ggctgccgac ccagattata cacatgaaag taaagaaata
     6421 agaaaaggcc ttacactgca tctcttctat aaatcaacaa ccccagccaa cgatagtgcc
     6481 tggcataata ttttatgggc agcagaaaag gtaatgccct acatagaaaa aagatttggc
     6541 aagtacccat atccacaata ctcctttatt caaggtggtg atggtggaat ggaatatgct
     6601 atggctactt taataaaagg aagtggttta ggtacagttt ttcatgaatg gatgcatagc
     6661 tggtaccaat ttgttttagc aaacaacgaa agcctttatg gatggatgga cgaaggtttt
     6721 acaacttttg ctgaagatga agtaacggaa tggtataata aaaactatgc atggcaaagc
     6781 ccatggataa cagaaaaagc aaaacaaaaa ataatttttc aaaataaaaa taacgatacc
     6841 gaactaccat taaagcaata taacaattat gctgcttatt ttaatttggt gaaaagccct
     6901 ttcgaagaac cattatcaac ccatgccgat cattttaaca ccaactttgg atactcttat
     6961 gcttcatata taaaaggctc agtgttctta acacaattgg gttatatcac cagcgattca
     7021 gtgagagata aaattttatt agcctattat gacacctgga agtttaaaca tcctaatgta
     7081 aacgatttta ttagagttgc agaaaaagta agtgggctag aattacaatg gtacaaagag
     7141 tactgggtat atacaactaa aaatata
//
LOCUS       DAOQPK010000098         7152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_5906_length_7152_cov_4.281527, whole genome shotgun sequence.
ACCESSION   DAOQPK010000098 DAOQPK010000000
VERSION     DAOQPK010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7152
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5906_length_7152_cov_4.281527"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PLU36_06005"
     CDS             <1..556
                     /locus_tag="PLU36_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="OstA family protein"
                     /protein_id="HMZ46340.1"
                     /translation="EAYSNVKIFSDSMQAIGDSLFYSLKDSIFRLYKNPIAWSNVNQI
                     TGDTIYLYTQNKKPEKIRVFENALAISHAGSNYYNQVSGRTINGYFVDGKLDSLRCKG
                     SPASSVYYGVDDYGKFFGVNVATCDVIDLFFKNGEPNKVVFKNNLDGITYPMQQVNHD
                     LIKVKGFKWLQHKRPKTKYEILSY"
     gene            622..1812
                     /gene="nhaA"
                     /locus_tag="PLU36_06010"
     CDS             622..1812
                     /gene="nhaA"
                     /locus_tag="PLU36_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HMZ46341.1"
                     /translation="MLQFLKNKFVNPLKEIIHDSRSIGIVLLACTILSLVLANISSIN
                     LTYTSFWNFSFNQSNEHSIPIGFLNLPNSILIIINDFLMAFFFFLAGMEIKRELTNGE
                     LSSYKKSILPIIAAIGGMLFPAIIYFAFNISTNHLHGWAIPTATDIAFTLGIASLLGK
                     RVPLSLKIFITALAIIDDLGAILVIAFFYGSHLHFTYLFISLLLTLLLILINKFKNKF
                     VILHIIVAFLLWYCMFNSGIHATLAGVIVAFIVPAKSLSKLEMKLHTPVYFIVLPIFA
                     LANTAIIFPNNIPALLNTSLSWGIIAGLVFGKPLGIFIASFITIKTKLAQLPTKTNYM
                     QLAGAGILCGVGFTMSIFISSLAFNEPINQDTAKIAVLFASSLAIILGIVWFKIFAKP
                     YRKN"
     gene            complement(1816..2508)
                     /locus_tag="PLU36_06015"
     CDS             complement(1816..2508)
                     /locus_tag="PLU36_06015"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HMZ46342.1"
                     /translation="MQLIKKNGIVLFWAVLFIDCFFVFSQQYQFHAITKVLLIPVLLF
                     YIILNSRKKHYNRSKTLIYTALICSWIGDILLLQNGDVFFIAGTTAFLTTHIFYSIFF
                     FRVHPINNARSYEVTIIATLVVGALIFAVANFLHNDLPKFFRIPFYVYAIAIGIMAIL
                     AANIYTNRGKQKLALQFFIPGAALFILSDVALATFKFKYTDENFLEVVVMITYGYAQC
                     FMAQGFAKYLKG"
     gene            complement(2557..3903)
                     /gene="tilS"
                     /locus_tag="PLU36_06020"
     CDS             complement(2557..3903)
                     /gene="tilS"
                     /locus_tag="PLU36_06020"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HMZ46343.1"
                     /translation="MKINLLENFLINWNNHFKEIANSNAHFFIAVSGGVDSVVLTDLM
                     SKTSFSFSILHCNFNLRNDESKRDEIFVQSLATKYNTTVLVEHFDTNSYALEHKLSIQ
                     VAARNLRYNWFKNIIEQNTTTNKKCFLLTAHQADDTIETVLMNIFRGTGLKGLHGISM
                     QNNYILRPLLFARRSEIEQYAKQNNLDWVEDSSNKKVDYTRNNFRLNIIPLIKEHFLT
                     VEENLLANIEKWKEAEVIYNLYINEQKQKLIEVKDNTIQIPILKLQQQKAVKTVLYEI
                     IANYGFTANQLKDAIELLDAATGKYIVSATHRILKNRKWVIITPLKSNNIQHFIIEQD
                     DKKIFFDSNSIEIKIIENSEIQSNSNIACLDLKKISFPLLLRKYKQGDYFYPLGMSKK
                     KKISRFLIDAKLSLTQKEDIWVMESNKKIIWLVGLRIDDRFKVTTQTKSILQLQVK"
     gene            4036..4500
                     /locus_tag="PLU36_06025"
     CDS             4036..4500
                     /locus_tag="PLU36_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HMZ46344.1"
                     /translation="MSIEVGKPAPSFSLVDTERKEVKLSDFKDSNVLLLFFPLAFTSV
                     CVKELCSVRDNIALYNNANAQVLGISVDSPFALAKFKAEQNLNFPLLSDFNKTASTSY
                     HSIYETFLGMNGVGKRSAFVIDKSGTIRYAEVLENAGDLPNFEAINKVLETL"
     gene            complement(4523..6556)
                     /locus_tag="PLU36_06030"
     CDS             complement(4523..6556)
                     /locus_tag="PLU36_06030"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46345.1"
                     /translation="MKFIAIKKTILLFTCIVLLQLISTAQRRRITADGTTGTTVTHDA
                     QGRPLPPKPKGTTDSLQKRDRYADSITIFYRYYDSSSIRFLDSTINDFAKRFNQPYFY
                     QNNGSYGTASRSLLFMPTLKAGFDVGINQYNIYDYTVEDTRFFQTTRPYTELAYVLAT
                     KAEQTINVLHTQNKKDNFNFALEYKFINSPGSYKTQNNNHNNIRVNANFKTNKRKYAA
                     QLIVISNKHIVSENGGLRDASFLDSLSFNDPFQLETRLGASGAYSQNPFNTNISTGNK
                     YKNNIFLFKHYYDVGKKDSTLNIQDSIYHKIFYTRFRLQHTLQSKSYSYQFVDANVEQ
                     TNYIKYFNFTVPNNNSISVENKWNVVENQFSIITFPDKNNQAQFLKLSAALQNIHRKD
                     SATSSNNHNFFISGEYRNKTRNNIWDVDAAGSLYITGLNKGDYEATVQLKKIISNKLE
                     FLQLGFKNVNRNPSLIFNSNHNYWVLPNLVTKKENTILLSAQYNNPKKYLSLSAKYYL
                     VNNYTYFDSFFSAKQESTLFYVLQISAEKKIKLSKNFNWYAEVHFQQSTANAPINVPM
                     FLTRNRLAFEGNFFTNLFLSTGIELKYYTNYKSAGYSPFNGQYFYATNYTITNRPELD
                     VFLHFRIKSFKAYLRLENVQSLIPGNGKNNFALQNYANQKLWFRTGIWWNFVN"
     gene            complement(6609..7106)
                     /locus_tag="PLU36_06035"
     CDS             complement(6609..7106)
                     /locus_tag="PLU36_06035"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HMZ46346.1"
                     /translation="MVAFESGVTDTVDPLAGSYFIESLTNEVEQKAWQLIQKIDAMGG
                     SVSAIEEGFMQNEIAKSSYDYQCNIESNQKIIVGVNKFTIDKEEAIPAFKIDDSIRQV
                     QSNKLQNLKDKRNNQKATNSLKAIEAAAINNTNLMPVVIEAVENYCTLGEIADCLRKV
                     FGEYK"
BASE COUNT         2423 a         1064 c          979 g         2686 t
ORIGIN      
        1 tgaagcttac agcaatgtga aaatattttc agactctatg caagccattg gtgatagttt
       61 attttacagt ttaaaagact ctattttcag gctatacaaa aacccaattg catggtctaa
      121 tgtaaatcaa ataacaggcg atacaattta tttatataca caaaataaaa aaccagaaaa
      181 aattcgtgtt ttcgaaaacg cattagcaat aagtcatgca ggaagtaatt actacaacca
      241 ggtttcgggt agaacaatta atggctactt tgtggacggc aaattagatt ccttaagatg
      301 caaaggcagc cctgcctcta gcgtttatta tggagtggat gattatggaa aattttttgg
      361 tgttaacgtt gccacttgcg atgtaattga tttatttttt aaaaacggag agcccaataa
      421 agtagttttt aaaaataatt tagatggtat aacttatcct atgcaacaag taaatcatga
      481 tttgataaaa gtaaaaggct ttaaatggtt gcaacacaaa cgccctaaaa ctaaatatga
      541 aattttgagt tattaaggat tgtaggaaaa atttcttctt aaatcgtttt acattctaaa
      601 ttctttcttt tactttagat tatgttacaa tttcttaaaa ataaatttgt aaacccttta
      661 aaagaaataa tacacgatag ccgttccatt ggtattgtgt tattagcttg taccatactt
      721 tcattagttc tagccaatat ctcttctatc aatttaacct atactagttt ctggaatttt
      781 agtttcaacc aaagtaatga gcatagcata ccaattgggt ttttaaactt accaaattca
      841 atcctcatta ttatcaacga ttttttgatg gcattctttt tctttttagc tggtatggaa
      901 ataaaaagag aattaaccaa tggagaattg tcttcctata aaaaatctat cttacctata
      961 attgcagcta taggcggtat gttgtttcct gcaattattt attttgcttt taatatctca
     1021 accaaccatt tgcatggttg ggcaatacca acagcaactg atattgcatt taccttgggt
     1081 attgcatctt tattaggtaa aagagttccc ctttctttga aaatttttat tacagcatta
     1141 gcaattattg acgatttagg tgccatttta gtaattgcct ttttctatgg atcacattta
     1201 cattttactt atttatttat aagtttacta ctaaccttat tactaatact aataaataaa
     1261 tttaaaaaca agtttgtaat actgcatatt atagttgctt ttttactttg gtattgtatg
     1321 tttaactctg gcatacatgc tacacttgca ggtgtaattg ttgcttttat tgtaccagcc
     1381 aaaagcttaa gtaaattaga aatgaaatta catacccctg tttactttat agttttaccc
     1441 atctttgcct tggccaatac agcaattatt tttccaaata atattcctgc tttattaaac
     1501 acatctttaa gttggggtat tattgcaggt ttagtttttg gtaagccctt aggtattttt
     1561 attgcaagtt ttataacaat aaaaactaaa ttagctcaac tacctaccaa aactaactat
     1621 atgcaattag caggtgcagg tattttatgt ggagttggtt ttaccatgag catctttatt
     1681 tcttcacttg catttaacga accaattaat caggatacag caaaaattgc agttttgttt
     1741 gcctctagcc ttgctatcat tttaggaatt gtttggttta agatttttgc caaaccatat
     1801 cgaaaaaact aagtactatc cctttaaata tttggcaaac ccttgtgcca taaagcattg
     1861 ggcataacca taggttatca ttactacaac ttctaaaaag ttttcgtcgg tgtatttaaa
     1921 tttaaaagtt gccaaagcaa catcagataa aataaataat gcagccccag gaataaaaaa
     1981 ttgtaatgcc aatttctgct tacccctatt agtataaata ttggctgcta aaattgccat
     2041 aatgccaatt gcaatagcat acacataaaa tggtattcta aaaaacttgg gtaaatcgtt
     2101 gtgtaaaaaa tttgcaacag caaatattaa tgcacctaca actaatgttg caattattgt
     2161 tacttcgtaa cttcttgcat tgttaatagg gtgaacccta aaaaagaaaa tagaataaaa
     2221 aatatgggtt gtcaaaaatg ctgttgttcc tgcaataaaa aaaacatctc cattttgtag
     2281 tagcaaaata tctccaatcc aagagcaaat taaagctgta taaataaggg ttttagaacg
     2341 attataatgt ttttttctag aatttaaaat tatataaaac aataaaacag gtatcaataa
     2401 aacctttgtg attgcatgga attgatattg ttgagaaaaa acaaaaaaac aatcaataaa
     2461 aagaacagcc caaaaaagca caataccatt tttttttatt aattgcattt agttttattt
     2521 taattttaaa tataataatg ctgcaaaaat aactaactat ttaacttgaa gttgtaaaat
     2581 gcttttggtt tgggttgtta ctttaaatct atcatcaatt cttaacccaa caagccaaat
     2641 aattttctta ttgctttcca ttacccaaat atcttccttt tgcgttaaag aaagtttagc
     2701 atctattaaa aaacgagaaa tttttttctt tttagacatt cctaaagggt aaaaataatc
     2761 tccttgttta tattttctca gcagtaaagg aaaacttatc ttttttaaat ctaaacatgc
     2821 aatgtttgaa ttagattgta tttctgaatt ttctataatc ttaatttcaa tggaattaga
     2881 atcaaaaaat atttttttat catcttgttc aattataaaa tgctgaatat tgttagattt
     2941 tagtggcgta ataattaccc attttctatt tttaagtatc ctatgcgttg cagaaacaat
     3001 atatttacca gtagcagcat ctaataattc aatagcatct ttcaattgat tagctgtaaa
     3061 accatagtta gcaataattt catataaaac tgttttaact gctttttgtt gttgtagttt
     3121 taatatagga atttgaattg tattatcttt aacttctatc agtttctgct tttgttcatt
     3181 tatataaagg ttgtaaataa cttctgcttc tttccatttt tcaatattgg ctaataaatt
     3241 ctcttcaaca gttaaaaaat gttccttaat taatggtata atatttaacc taaaattatt
     3301 tcttgtataa tccacttttt tattagaaga atcttcaacc caatctaaat tattttgttt
     3361 ggcatattgt tcaatttcac tccttctagc aaataatagt ggccttaaaa tgtaattgtt
     3421 ctgcatacta attccatgaa gtccttttaa accagtacct ctaaaaatat tcatcaaaac
     3481 tgtttcaata gtatcatctg cctgatgtgc tgtaagtaaa aaacattttt tattggttgt
     3541 agtattttgt tcaattatat ttttaaacca attgtacctt aagtttcttg cagcaacttg
     3601 tatagataat ttgtgttcta aagcataaga attagtatca aaatgttcta ctaaaacagt
     3661 ggtattgtat tttgtagcta atgattgtac aaatatttca tctcttttac tttcatcatt
     3721 tctcaaatta aaattgcagt gtaaaataga aaaactaaaa gaagttttag acattaaatc
     3781 tgttaatact acactatcta cgcccccact tactgcaata aaaaaatgag cgttactatt
     3841 tgcaatttct ttaaaatggt tattccaatt aattaaaaaa ttctcgagca agtttatttt
     3901 cacttgtata atttactatt ttggcaaaaa tattttatat tagtcaaata accatcaaaa
     3961 caaatgtttt acagcatgcc taagcttgtt tattacatat ttgaattgta gatttacatt
     4021 tcaaaaaaaa atattatgag tattgaagta ggcaaaccag caccttcttt ttctttagta
     4081 gataccgaaa gaaaagaagt aaaactttct gattttaaag attctaatgt attactatta
     4141 ttttttcctt tagcttttac aagtgtatgc gtaaaagagc tatgtagtgt aagagataat
     4201 attgcattgt acaataatgc aaatgcacaa gtgcttggca tttcggttga ttctccattt
     4261 gctttagcaa aatttaaagc agagcaaaat ttaaatttcc ctttattaag cgattttaat
     4321 aaaacagcat ctacaagtta tcattccata tatgaaactt ttttaggcat gaatggtgtt
     4381 ggtaaacgtt ctgcttttgt aatagataaa tcgggtacta tacgttatgc agaagtatta
     4441 gaaaatgctg gtgatttacc aaattttgaa gcaattaata aagttttaga aacactttaa
     4501 ctttacttaa accgatctga atttaattta caaaattcca ccaaatgcct gttctaaacc
     4561 atagcttttg gttggcataa ttttgtaaag caaaattatt tttaccatta cctggaatta
     4621 agctttgaac gttttctaat cttaaatagg ctttaaaact tttaattctg aaatgtaaaa
     4681 acacatccaa ttcaggtcgg tttgttattg tataattggt tgcataaaaa tactgaccat
     4741 taaaaggaga ataaccagca cttttataat tggtataata ctttaactct atccctgtag
     4801 ataaaaatag gttagtaaaa aaattaccct caaaagccaa tctatttctt gttaaaaaca
     4861 taggtacatt tattggtgca ttggctgtag attgttgaaa atgaacctcg gcataccaat
     4921 tgaaattttt agataattta attttcttct ctgcactaat ttgcagaaca taaaacaaag
     4981 tactttcttg tttggccgaa aaaaaactat caaaataagt atagttattg actaaataat
     5041 actttgcaga taacgataaa tatttttttg gattattata ttgagcagat agtaaaattg
     5101 tgttttcttt tttggtaacc aggttaggca gtacccaata attatggtta ctattaaaaa
     5161 ttaaagatgg atttctattt acatttttaa accctaattg taaaaattca agcttgttgc
     5221 taattatttt ttttaattga actgttgctt cataatctcc tttgtttaaa ccagtaatat
     5281 ataaactacc agcagcatct acatcccaaa tattatttct tgttttattt ctatactcac
     5341 ccgatataaa aaaattatga ttattactgc ttgttgcaga atcttttcta tgaatatttt
     5401 gcagggctgc actcagtttt aagaactgag cctgattatt tttatctgga aaagtaataa
     5461 tgctgaattg attttctaca acattccatt tattttctac actaatacta ttattattag
     5521 gtacagtaaa attgaaatat ttaatgtaat tagtttgctc aacattggca tctacaaact
     5581 gataactata gcttttagat tgtaatgtat gttgcaacct gaatcttgta taaaaaattt
     5641 tatgataaat ggagtcttgt atatttaatg tagaatcttt tttacctaca tcatagtaat
     5701 gtttaaataa aaaaatattg tttttatatt tattacctgt tgaaatatta gtattaaaag
     5761 ggttttgaga ataagcacca gatgcaccta accttgtttc aagttgaaaa ggatcgttga
     5821 aagaaaggct atctaaaaaa ctggcatctc ttaatccacc attttcagaa actatatgtt
     5881 tgtttgaaat aacaattaat tgtgcagcat acttacgctt attagtttta aaattggcat
     5941 taaccctaat attattatga ttattatttt gtgttttata actaccaggg ctattaataa
     6001 acttatactc taaagcaaaa ttaaaattat cttttttgtt ttgagtatgc aacacattaa
     6061 tagtttgttc tgctttggtg gctaacacat aagcaagctc tgtatatggc ctggtggttt
     6121 gaaaaaatct tgtatcttct acagtataat cataaatatt gtactggttt attccaacat
     6181 caaaccctgc ttttaaagta ggcataaata ataacgacct tgaagctgta ccataacttc
     6241 cattgttttg ataaaaatat ggctgattaa atcttttagc aaaatcgtta atggtagaat
     6301 ctaaaaatct gatagaagaa gagtcgtaat acctgtaaaa tatagtgata gagtctgcat
     6361 atctatctct tttttgtaga gagtctgttg tgcctttagg ttttggaggt aaaggcctgc
     6421 cttgtgcatc atgtgttaca gttgtgccag ttgttccatc tgcggtaatc cttcttcttt
     6481 gtgcagtaga aataagctgc aacaatacaa tacaagtaaa aagtagtatt gtttttttta
     6541 ttgcaataaa tttcatagct gcaaattaac tgtaatggct gcaaatgaag tgtaaaaatt
     6601 agcgaatact atttgtattc tccaaaaact tttcttaaac aatctgcaat ttcgcctaag
     6661 gtgcaataat tttcaacagc ttcaattact acaggcatta aattagtgtt attgatagct
     6721 gctgcttcaa ttgcttttaa agaattagtg gctttttgat tatttctttt atcttttagg
     6781 ttttgaagtt tatttgattg aacctgtcta atgctatcat caattttaaa agcaggaata
     6841 gcttcttctt tgtctattgt aaatttattt accccaacta taattttttg gttactttca
     6901 atattacatt ggtaatcgta ggagcttttt gcaatttcat tctgcataaa tccttcttca
     6961 atagcactta cactgccccc catggcatct attttttgaa ttaattgcca agctttttgt
     7021 tctacctcat tagttagaga ttcaataaaa taacttcctg caagtgggtc aacggtatct
     7081 gtaacaccac tttcaaaagc tactatttgt tgggttcttg ttgtcccatt aaattagacc
     7141 cactttcaat at
//
LOCUS       DAOQPK010000099         7149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_5913_length_7149_cov_4.369044, whole genome shotgun sequence.
ACCESSION   DAOQPK010000099 DAOQPK010000000
VERSION     DAOQPK010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7149
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5913_length_7149_cov_4.369044"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..112)
                     /locus_tag="PLU36_06040"
     tRNA            complement(40..112)
                     /locus_tag="PLU36_06040"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(77..79),aa:Lys,seq:ctt)
     gene            223..585
                     /locus_tag="PLU36_06045"
     CDS             223..585
                     /locus_tag="PLU36_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HMZ46347.1"
                     /translation="MIKQILSYSLLVIILTSCSNYITKTGKASFYSDKYKGKKTANGE
                     TYKPNEFTAAHKKLPFGTKVKVTNLNNNKTVTVRINDRGPFVAGRIIDLSRVAASEIG
                     MINNGVVKVKIKYKRNKD"
     gene            606..1331
                     /locus_tag="PLU36_06050"
     CDS             606..1331
                     /locus_tag="PLU36_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HMZ46348.1"
                     /translation="MNKPYLHTILSPELLNIYDINDSIVVIIDVFRATSTIATALYNG
                     AEKIIPVDSVEKCIAIGNATPNSITAGERDGKVIEGLQYGNSPAEYPRKFIEGKTLVL
                     TTTNGTKLLHMALNKGASEIITGSFPNLNAVCNFLRTQKKNIFLGCSAWKNRFNLEDT
                     LFAGAVIEKVKSSFHIHCDSSLMAEQMYLHHKHDMFAFARLTTHWHRLSKFGLDKDLE
                     YCVTNNVADVLPIYKNGSLIALT"
     gene            1405..2028
                     /locus_tag="PLU36_06055"
     CDS             1405..2028
                     /locus_tag="PLU36_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HMZ46349.1"
                     /translation="MTNFIPIFPVSTVVYPNETINLHIFEPKYIQLINECFETNKVFG
                     IPVIINNTIAEMGTTLKVNSIIKKYDDGKLDIITEGVDVFSILEIIHIIPNKLYSGAI
                     VTYPENEKGLLTNKMKQIYKQVLALHKFLQVTKKFKREVEEMISYDIAHHICLSVEEE
                     YNLLQLLKENQRLEFLKRHLLKMLQTMQQTEKLKEKIKLNGHFRELR"
     gene            2045..3364
                     /gene="dacB"
                     /locus_tag="PLU36_06060"
     CDS             2045..3364
                     /gene="dacB"
                     /locus_tag="PLU36_06060"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HMZ46350.1"
                     /translation="MKNSTLFFCRNKRLQIVVCIIGLLLIVNRGNTQAAVEKFINNSI
                     FASAHIGLAVYEPATDKYIYSYQSQKNFIPASNLKIFTCYAAIKNLEDSLTGLFYTQE
                     DSNFVVQFSGDPTLLHADFKQHPVIDFFTKNKFITVIKANWDNKYYGNGWAWDDFEAD
                     YMPELSTIPIYGNTVQFLKDENRVIVIPRWFKDSLTFYGNTQNGKYNIERNIHSNQFK
                     LVNSDQKFFQKNIPFITSDVLAIELLQDTIFKPIDYTDADVIGNVEWHKLKSQPTDTV
                     LKYMMQRSDNFYAEQLLLMIGNEKFKETKPNKIINSLLQNTFNGLPQKIRWEDGSGLS
                     RYNLVTPEDIVWVLTKMKNDIGAERVNTIFPTGNQGTLKGMFKNYTENIFAKTGSLSN
                     NFSLSGYIKTKSNKDLVFSILINNHQASTNEIKNAVEQFLINLIEKE"
     gene            complement(3416..4162)
                     /locus_tag="PLU36_06065"
     CDS             complement(3416..4162)
                     /locus_tag="PLU36_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HMZ46351.1"
                     /translation="MEYNTQRNHLEMREYGRHIQKMVEYLLSIEDEEKRNNQAKAVIE
                     LMGFLNPHLRNVEDFKHMLWDHLFVISNFQIKVDSPYPIPQKETYKEKGERLPYPKRY
                     PRYAHLGKNLETVIDKALAEEDPEKKSGFANAIAYYMKLSYSNWHKELVHDDTIRAEL
                     DEITGGQLEFSNTPFIKHRNQNFERDDYGRGGSNQQRRQSFSNRNGNNNNGRNNNNNR
                     NNNNRNGNNNNGRNNNNNRNNNNRNFKKRY"
     gene            complement(4208..6031)
                     /locus_tag="PLU36_06070"
     CDS             complement(4208..6031)
                     /locus_tag="PLU36_06070"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HMZ46352.1"
                     /translation="MDKFIVSARKYRPQTFDTVIGQSHITTTLKNAITSNQLAHAFLF
                     CGPRGVGKTTCARILAKTINCENLQPDTEACNECNSCKSFNDGASLNIHELDAASNNS
                     VDGIRALVEQVRFAPQGGKYKIYIVDEVHMLSSGAFNAFLKTLEEPPAYAKFILATTE
                     KHKILPTILSRCQIFDFKRITTNDTVQHLQEIITKENINADKASLQVIAQKSEGCMRD
                     ALSILDKIVSFSNGEVTYTNTIEHLNILDHDYFFKIYQCLQNQDFATAMLLYDEINRK
                     GFEGDMLLSGFAEFIRNILVCKDEKSVKLLDVVVGLEDKYVTLAQQASLSYLISILNI
                     LNESELNYRMARNKRLHIELTLIKLNFLKQAIELTIDNEGSLVKKKIIHNNPVLKFKQ
                     IPSLQVKPKFASTTEEAKLYVQEPTIKQVKVTTPQQTENNTKTASTTTTSSNDIPPNN
                     INNSGNKRLLDILREKAGDKYNVTAILEAEGLVLEKLKTCWHNYALTLKQQGNKHSTV
                     KTFEIAELEIIDDLNFIIKVSAITQQKFIEQEKTNLLQSIHTAFNNRAINFTFQLTDT
                     LQQQDVPLHMKLNSKQKFEYLANKYPLLKELKEKLNLEIDY"
     gene            complement(6099..6461)
                     /locus_tag="PLU36_06075"
     CDS             complement(6099..6461)
                     /locus_tag="PLU36_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HMZ46353.1"
                     /translation="MQPFITKTLVTAAAVIIASFLLSGVHVDSSLTAIMVAIVLGLLN
                     TFIKPLLIILTIPITIFTLGLFLIIINILMIKWAASLVPGFTVENWWAAFWFSIIVSI
                     FSSIIQNITTTKNKSEEE"
     gene            complement(6479..>7149)
                     /locus_tag="PLU36_06080"
     CDS             complement(6479..>7149)
                     /locus_tag="PLU36_06080"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HMZ46354.1"
                     /translation="FVAGKLPTNINNLLNKYFGNLPFNYNKELQKNYLPVCNKTKKHS
                     IINDTDGVQNAIRIARSFPNKIHPDYNNAQILNTILGGFFGSRLMKNIREDKGYTYGI
                     HSFIQNHFHQTAWMISTEAGKDVCNATIEEVYKEMKLLCNEPISNDELFLVKNYLMGN
                     LLSNQSGPFQVIAKWKSLILNNLKEDFYYNSIHTIKTTNAKDLQEIANKYLNPADFYE
                     LTVL"
BASE COUNT         2441 a          997 c         1046 g         2665 t
ORIGIN      
        1 attaagttgc tttgcacctt taatttcgtt taacttttgt gacccctcag ggactcgaac
       61 cctgggccta ctgattaaga gtcagtagct ctaccaactg agctaaggag tctgaaaatt
      121 tttctaaata aagttaaggt gcaattttgt tttcaacaat aattttgtat ttgttgaaaa
      181 tttaatctac taaaatttag tatgcttttt tagttttaac ttatgataaa acaaatttta
      241 tcttatagtt tattggtaat tatattaacc tcttgtagta attatattac taaaactggt
      301 aaggcttctt tttattcaga taaatataaa ggcaaaaaaa ctgcaaatgg agaaacgtat
      361 aaacccaatg aatttactgc agcacacaaa aaattacctt ttggtactaa agtaaaagtt
      421 actaatttaa ataacaataa aacggtaact gtacgtataa acgatagagg cccatttgta
      481 gctggcagaa ttattgacct tagcagagtg gctgctagtg aaattggaat gataaataat
      541 ggtgttgtaa aagttaaaat taaatacaaa agaaacaaag attaatagca caatatattt
      601 gcagcatgaa taaaccctat ttacacacta ttttatcgcc tgagctatta aatatttacg
      661 atataaatga tagcattgtt gtaataatag atgtatttag agcaacctct actattgcaa
      721 ctgcattgta taatggtgca gaaaaaatta ttcctgtaga ttctgttgaa aaatgtatag
      781 ccattggtaa tgcaacacca aacagtataa ctgctggcga acgagatggc aaagtaattg
      841 aaggattaca gtatggcaat agccctgcag aatatcctag aaaatttatt gaaggtaaaa
      901 cgcttgtgtt aactacaact aatggcacca aactactaca catggcatta aataaaggtg
      961 caagcgaaat tattactggc tcttttccaa acttgaatgc tgtttgtaat tttttaagaa
     1021 cacagaaaaa aaatattttt ttaggctgta gtgcatggaa gaatagattt aatttagaag
     1081 atactttatt tgctggtgct gtaattgaaa aagtaaaatc ttctttccat attcattgcg
     1141 atagtagttt aatggcagaa caaatgtatt tacatcataa gcatgatatg tttgcttttg
     1201 cacgtttaac cacacactgg cataggctaa gcaaatttgg gttagataaa gatttagaat
     1261 attgtgttac taataatgtg gcagatgtgt taccaattta taaaaatggc tcgttaattg
     1321 cactaactta attcatatac tattttaagt tttcatttca tttaatagta ggctcttttt
     1381 tattcaaata attactttta atgcatgaca aactttattc ctatatttcc tgttagtacc
     1441 gttgtttatc ctaacgaaac cattaaccta catatatttg agccaaagta tatacaatta
     1501 attaatgaat gttttgaaac aaataaagta tttggtattc ctgtaattat caataatact
     1561 attgccgaaa tgggcacaac gcttaaagta aattcaatta taaaaaaata cgatgatgga
     1621 aaattagata taataacaga gggagtagat gttttttcaa ttttagaaat aattcatatt
     1681 atacccaaca aattatatag tggagccatt gttacatatc ctgaaaatga aaaaggctta
     1741 ctcaccaata aaatgaaaca aatttacaaa caggttttgg cattgcataa gtttttacaa
     1801 gttacaaaaa aatttaagag agaggtagag gaaatgataa gttatgatat tgcacatcat
     1861 atttgtttat cggttgaaga agagtataat ttattacaat tgttgaaaga aaaccaaagg
     1921 ctagagtttt taaaaaggca tttattaaaa atgctacaaa ccatgcaaca aacagaaaaa
     1981 ttgaaagaaa aaataaaact gaatggccat tttagagagt tgagataaca aataaaattt
     2041 aagtatgaaa aacagtacac tatttttttg cagaaataag aggttacaaa ttgtagtatg
     2101 tattattggt ttattgctaa ttgttaatag aggtaataca caggctgctg tagagaaatt
     2161 tattaataat tcaatatttg catcggcaca tattggattg gctgtttatg aaccagcaac
     2221 agataaatat atttatagtt accaatctca aaaaaatttt atacctgcaa gcaacctaaa
     2281 aatttttact tgttatgctg caataaaaaa tttagaagat agtttaacag gcttatttta
     2341 tacacaagaa gatagcaact ttgttgtaca gttttccgga gacccaactt tacttcatgc
     2401 agattttaag caacatcctg taattgactt ctttactaaa aacaaattca ttactgttat
     2461 aaaagcaaat tgggataaca aatattatgg taatggctgg gcttgggatg attttgaagc
     2521 tgattacatg cctgaacttt ctactattcc catatatggc aatacagtac aatttttgaa
     2581 agatgaaaat agagtaatag ttattccacg ttggtttaaa gactctttaa ctttttatgg
     2641 taatacgcaa aatggtaaat ataatattga aagaaacatt cattctaatc agtttaagtt
     2701 agtgaatagt gatcaaaaat tttttcaaaa aaatattcca tttattacat cagatgtatt
     2761 ggctatagag ttattacaag atacaatttt taaaccaatt gattatacag atgccgatgt
     2821 aattgggaat gttgaatggc ataaattaaa atctcaacca accgatactg ttttaaaata
     2881 catgatgcaa agaagcgata acttttatgc agaacaactt ttattgatga tagggaatga
     2941 aaaatttaag gaaacaaaac ctaataaaat tattaattct ttattacaaa atacttttaa
     3001 tggcttacct caaaaaatta gatgggaaga tggaagtggt ttgagtaggt ataatttagt
     3061 aactccagaa gatatagttt gggtacttac aaaaatgaaa aatgatattg gagcagaaag
     3121 agtaaatact atttttccta ctggcaacca aggtacttta aaaggaatgt ttaaaaatta
     3181 tacagaaaat atttttgcca aaacaggctc attaagcaat aatttttctt taagcggata
     3241 tataaaaact aaatctaata aggatcttgt attttcaata ttaatcaata atcatcaagc
     3301 atctactaat gaaataaaaa atgcagtaga gcagtttttg attaatttga tagagaaaga
     3361 gtaaaaccaa aataaaaaag gcactttatt taaagtgcct ttttgaaaaa atgtattaat
     3421 accttttctt aaaatttcgg ttgttgttgt tacggttgtt attattattt cttccgttat
     3481 tattatttcc gtttcggttg ttgttgttac ggttgttatt attatttctt ccgttattat
     3541 tatttccgtt tcggttgcta aaactttgtc ttcgttgctg gttgcttcct ccacgtccat
     3601 aatcatctct ttcaaagttt tgatttctat gttttataaa tggtgtatta ctaaattcta
     3661 gttgaccacc agtaatttca tctaactctg ctctaatggt atcatcatgt actaactctt
     3721 tatgccaatt actataagat agtttcatat aataggcaat agcattagca aaaccagatt
     3781 ttttttctgg atcttcttca gctaaggctt tgtcaataac agtttctaaa tttttaccta
     3841 aatgagcata tcttggatat cgttttgggt aaggtaatct ttctcctttt tctttataag
     3901 tttctttttg tggaattggg taagggctat caactttaat ttgaaaattg ctgattacaa
     3961 ataaatgatc ccatagcatg tgtttaaaat cttctacatt tcttaaatga gggtttaaaa
     4021 agcccattaa ttctataaca gctttagcct gattgtttct tttctcctca tcttctattg
     4081 acaaaagata ctccaccatt ttttgaatat gcctaccata ttctctcatc tctaaatggt
     4141 ttctttgtgt attatactcc atattgcaaa tgtaggggtt gatatataaa taaatttaaa
     4201 ttacctttta gtaatctatt tctaagttta atttttcttt caattcttta agtaatggat
     4261 atttattagc aaggtattca aatttttgtt tgctatttaa cttcatgtgt aagggtacat
     4321 cttgctgttg taaagtatct gttaattgaa aagtaaagtt aattgctcta ttattaaatg
     4381 ctgtatgaat actttgtaat aagtttgttt tttcttgttc aataaacttt tgttgtgtaa
     4441 tggccgaaac tttaattata aaatttaggt catctataat ttctaattca gctatttcaa
     4501 aagttttaac tgttgaatgt ttatttccct gttgttttaa tgttaatgca taattatgcc
     4561 aacaagtttt aagtttttct aataccaaac cttctgcttc taaaattgct gtaacattat
     4621 atttatcacc agctttttct cttaaaatat ctaacaatct tttattgccc gaattattaa
     4681 tattatttgg tggtatatca ttagaagaag tagttgtagt agatgctgtc ttagtattat
     4741 tttcagtttg ttgtggtgtg gtaactttaa cttgttttat tgtaggttct tgtacataca
     4801 attttgcttc ttctgttgtt gaagcaaatt tgggctttac ttgtaaagag ggtatttgtt
     4861 taaattttaa aaccggatta ttgtggatta tttttttttt aacaaggcta ccttcattat
     4921 ctatagttaa ttcaattgct tgctttaaaa agttaagttt tataagtgta agttctatat
     4981 gtaacctttt atttctagcc attctatagt ttagttcaga ctcgtttaaa atatttaata
     5041 tgcttattaa ataagatagg cttgcttgtt gtgccagtgt tacatacttg tcttctaacc
     5101 ctacaacaac atctaatagc tttaccgatt tttcatcttt acacaccaaa atatttctaa
     5161 taaattcggc aaagccggat aaaagcatat ctccttcaaa accttttcta tttatttcat
     5221 catataataa cattgctgta gcaaaatctt gattttgcaa gcattgataa attttaaaaa
     5281 aataatcatg gtctaaaatg ttaagatgct caattgtgtt ggtataagtt acttcgccat
     5341 ttgaaaagct tacaatttta tctaaaatac ttaaagcatc tctcatacag ccttcgcttt
     5401 tttgagcaat aacttgtaac gacgctttgt ctgcattaat attttctttt gtaattattt
     5461 cttgtaagtg ttgtacagta tcgtttgtag ttattctttt aaagtcgaag atttgacaac
     5521 gacttaaaat tgttggtaaa attttatgtt tttcggttgt ggccaaaata aattttgcat
     5581 acgccggcgg ctcttctaat gtttttaaaa aagcattaaa tgcaccagaa gataacatgt
     5641 gcacctcatc tacaatatat attttgtact tgcctccttg tggtgcaaaa cgtacctgct
     5701 ctactaatgc tctaatacca tctacactgt tgttactggc agcatctaat tcatgaatat
     5761 ttaaagaagc cccatcatta aaacttttac agctattaca ttcattgcag gcttctgtat
     5821 ctggttgtaa gttttcgcaa ttaattgttt tggctaaaat tcttgcacaa gttgttttac
     5881 caacaccacg tggcccacaa aataaaaatg catgtgcaag ttggttgctg gttattgcat
     5941 tttttaaagt tgttgtaata tgagattgcc caattacagt atcaaatgtt tgtggtcgat
     6001 attttctggc agaaacaata aacttatcca tagttggcaa tttagcagat ttatttttaa
     6061 ttgaactatt caactataat ttacaccaaa atatttatct actcttcttc tgatttattt
     6121 tttgttgttg taatattttg aataatggac gaaaaaatag aaacaataat actaaaccaa
     6181 aatgcagccc accaattttc tacagtaaaa ccaggtacta aacttgctgc ccattttatc
     6241 attaatatat tgatgataat taaaaataaa ccaagtgtaa aaatggtaat tggtattgtt
     6301 aatattatta ataatggttt aataaaagta tttagtaaac ctaaaactat ggctaccata
     6361 attgcagtta aactactatc tacatgtacc ccacttaata aaaaagaggc aataattact
     6421 gctgctgctg ttactaatgt tttggttata aatggttgca tgattttatt ttttataatt
     6481 acaaaacagt aagttcataa aaatctgcag gatttaaata tttatttgct atttcctgca
     6541 aatcttttgc attagttgtt ttaatggtat gaattgaatt ataatagaag tcttctttta
     6601 aattatttaa tattaatgat ttccactttg caataacttg aaaagggcca ctttgattgc
     6661 taagtaaatt tcccatcaaa taatttttta ctaaaaaaag ttcgtcgtta cttattggtt
     6721 cgttacataa taatttcatt tctttatata cttcctcaat agttgcgtta caaacatctt
     6781 ttcctgcttc tgtacttatc atccatgcgg tttgatggaa atgattttgt ataaaagaat
     6841 gtatgccata ggtgtaacct ttatcttctc taatattttt cattaacctg cttccaaaaa
     6901 agccacccaa aatggtattt aatatttgag cattgttata atctggatgt atcttattag
     6961 gaaaacttct agcaattcta attgcatttt gaactccatc tgtatcgttt attatagagt
     7021 gttttttagt tttgttacaa acaggtaaat aatttttttg taattcttta ttataattaa
     7081 aaggcaagtt accaaaatac ttgttcaata agttatttat attggttggc agttttcctg
     7141 caacaaaaa
//
LOCUS       DAOQPK010000100         7102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_5963_length_7102_cov_5.252732, whole genome shotgun sequence.
ACCESSION   DAOQPK010000100 DAOQPK010000000
VERSION     DAOQPK010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7102
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5963_length_7102_cov_5.252732"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PLU36_06085"
     CDS             <1..405
                     /locus_tag="PLU36_06085"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HMZ46355.1"
                     /translation="VVMIYTTCKAACPILVSKMKSIEAKIDKKNIKDVSLVLVSIDPT
                     TDTPEKLKEFSITNKMNDPQWVFLTSNDAATQEFANVLSMKYKKISPLDFSHSNIISV
                     FNPLGELVSQEEGTIDVDKVVNTINETVKKNK"
     gene            537..1292
                     /locus_tag="PLU36_06090"
     CDS             537..1292
                     /locus_tag="PLU36_06090"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HMZ46356.1"
                     /translation="MDSITNTQEELDMRVLVPIERHKKLIQLFKELPVGNYFIFINDH
                     DPIPLYYEFRSIYGDVVGWEYLNKGGREWKVKVTRTEASQGREFTDISTLLDLRKANK
                     NEWKQIVFHRYGMMEQGDTMEIIAAENPTEIHGIFVQKFEGKHTWIYKKQTDTEVVVH
                     ITKKEKTGLGDDGYAVVNEYDLRPYPPAKRHDMFYEAFAEIKPGEAFEFYNDHDPLPL
                     YYQMEAESKDPFKWEYIIKGPEEWKVRVIKIKP"
     gene            complement(1350..1856)
                     /locus_tag="PLU36_06095"
     CDS             complement(1350..1856)
                     /locus_tag="PLU36_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46357.1"
                     /translation="MNKPLHDFFTKDHHRLEGYLVNVIEADNEINVEYYHLFRKGLLR
                     HIKMEEKILFPAAAKVNKELVQKIIPQFRLEHGALTALLVPPPTKAIINAIQFVLNKH
                     DFEEEKDGGLYDICEALTIAQTNTLLQQLIATEEVPLHPPNPAPIALESAIRALQRAG
                     YVYDEICK"
     gene            2003..2386
                     /locus_tag="PLU36_06100"
     CDS             2003..2386
                     /locus_tag="PLU36_06100"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HMZ46358.1"
                     /translation="MAKPIIYSCSGCSSAAQMANYMAVKLDRKDVAEMSCIVGVGGNV
                     KKLVKTAKSGRKIIVIDGCPLNCSKACLNNQGIEPDLHFELTKFGVKKQNHIDFDMEQ
                     ANNLLELIENKIDEAIVKETSLVLN"
     gene            2389..3624
                     /locus_tag="PLU36_06105"
     CDS             2389..3624
                     /locus_tag="PLU36_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HMZ46359.1"
                     /translation="MKNKIELLAPVGSFESLHAAIQAGANAIYFGVEQLNMRAKSINS
                     FMLDDLAAIQALCIPKNISTYITLNTVMYEHDMQLLKAILKEVKKHKITAVIASDFSV
                     MEYCKQLQIPLHISTQANVSNIESVKFFSTFSDAVVLARELTLKQVKQITDEIKRKKI
                     KGVSGQLMRIEIFIHGALCMAISGKCYLSLHSQNASANRGACTQNCRHAYKVTDIETN
                     EELVIDNEYIMSPKDLCTIDIIDQIIESGVSILKIEGRGKGPEYVYTVTKTYQEAITA
                     VEEKEYTKEKIDKWKTELEKVYNRGFWEGYYLGRKLGEWTLNPGSMATEKKVYIGKGK
                     KYFSKISVGEFVVETGKVEIGDTIIITSPNFGIVKENIEQLTVNGNNATVAVKGDSIT
                     LRCKNKISAKDKIYKLVQS"
     gene            3625..3849
                     /locus_tag="PLU36_06110"
     CDS             3625..3849
                     /locus_tag="PLU36_06110"
                     /inference="COORDINATES: protein motif:HMM:NF024763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HMZ46360.1"
                     /translation="MPNIIHYRSKCIGCNICYEMMPSTWRMSKKDGKATLLKAEQKRQ
                     TSVLQIDTNLIEEAKKVANACPVKIIKVNG"
     gene            complement(3846..4283)
                     /locus_tag="PLU36_06115"
     CDS             complement(3846..4283)
                     /locus_tag="PLU36_06115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HMZ46361.1"
                     /translation="MFSKTTEYALRATIYIAQNSSVVNKIGIDEIAKAIDSPKSFTAK
                     VLQLLTKDSRVISSVSGPKGGFYMTDEAKNLPVRSVLEATGESIVLEKCVLGLNKCSE
                     KKPCPMHSRYKDIKEQLVNLFTNEPISKLAEETKLGNVFIGNC"
     gene            4522..5736
                     /locus_tag="PLU36_06120"
     CDS             4522..5736
                     /locus_tag="PLU36_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HMZ46362.1"
                     /translation="MSHNILPIIKIGGVNHHNKEWIAIYCKKDFTINSIIKKIKGNTF
                     SASKQCWLIPYEKYNYDYLVKHLKELASIDDKLISIFKKEKEKKLTGITNKLSNQTLL
                     QISIQLPNQQKIHPVNKQVLEQVQQHLQLKAYSTSTRKTYLNEIAAFLATIKHVKASA
                     LTPNRIKDYLQYCYKELKLSENTMHSRINALKFYYEQVLKKDKFFYEIPRAKKPYKLP
                     NVLSEKEIVRLFNTLQNQKHKAILFIAYSAGMRVSEVVGLKISNIDSQRMQIKIEGAK
                     GKKDRYVNLSTIVLEVLRSYIKQCVPRPKKYLFESEIAGEPYSSRSAQKVFQRAKEKA
                     GISKNVTFHSLRHSFATHLLEKGVDIRYIQDILGHNNIKTTERYTHVKKEQIIQITSP
                     LDDLWLKGDIKI"
     gene            6107..6427
                     /locus_tag="PLU36_06125"
     CDS             6107..6427
                     /locus_tag="PLU36_06125"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ46363.1"
                     /translation="MTKKKLTTFGETIRQLREEQKLPQRKVAAILDIDTSLLAKYERN
                     VRQPSKELINKIAEIFKVDSEELIKEALTDKIAYQIIEEDADSKILRVAEDKVEYLKT
                     KKTK"
     gene            6424..>7102
                     /locus_tag="PLU36_06130"
     CDS             6424..>7102
                     /locus_tag="PLU36_06130"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HMZ46364.1"
                     /translation="MIEDINYALVEDVRPPIYTAMKYWGKKPHNIWRKYIENYTPENG
                     LFLDPFSGSAISAFEAIKANRKVIAFDLNPLTSFIIEIFATDFDKNKFEKEVKRISDT
                     FKTDEIYNKYFTTQSRKSNETTIVQSFKWEEDKIYELGIEATEKEIKEQNDLKKKKKI
                     EKVIKRYTSRPNTEDNLKGISMNEIQIPFWFPDKPFHNSPSFPASFISCLGGNNFTNL
                     WTKRNLYV"
BASE COUNT         2840 a         1042 c         1124 g         2096 t
ORIGIN      
        1 gtagtaatga tttatacaac ttgcaaggca gcatgtccaa ttttagtttc taaaatgaaa
       61 agtatagaag caaaaattga taaaaaaaat ataaaagatg tttcgctagt tcttgtatct
      121 attgacccta caactgacac ccctgaaaaa ttaaaagaat tttcaataac taataaaatg
      181 aacgacccac aatgggtttt tctaacctcc aatgatgctg ccacacaaga atttgctaat
      241 gttctttcta tgaaatataa aaagatttcg ccgttagatt tttctcattc aaatattatt
      301 agtgttttta accctttggg tgaattggta agccaggaag aaggcaccat tgacgtggat
      361 aaggttgtaa acactattaa tgaaacggta aaaaagaata aataatttta agggaaatga
      421 tattaatgta gtgtaaaaat ttcccaaata cacaaacctt ttggcaggaa aactataatt
      481 aagcacacgc tactccataa ttaaaaatta tctttgcaac gcaaaaaatt tattttatgg
      541 atagtataac aaatactcaa gaagaattag atatgagggt gcttgtgcca attgaaaggc
      601 acaaaaaact tattcaactt tttaaagaat taccagtagg caattatttt attttcatca
      661 acgatcacga ccctattcct ttatactacg aatttcgttc tatttatggc gatgtggtag
      721 gttgggaata tttaaataaa ggtggcagag aatggaaagt aaaagtaacc cgaacagaag
      781 cttctcaagg cagagaattt acagatattt ctacattatt agatttaaga aaagcaaata
      841 agaatgagtg gaagcaaatt gtatttcatc gttatggtat gatggaacaa ggcgatacaa
      901 tggaaataat tgcagcagaa aaccctacag aaattcatgg aatttttgtt caaaaatttg
      961 agggtaaaca tacttggatt tataaaaaac aaaccgatac agaagtagta gttcatatta
     1021 ctaaaaaaga aaaaacaggt ttaggagatg atggctacgc agtagtaaat gaatacgatt
     1081 taaggcctta tccgccagca aaaagacacg atatgtttta tgaagctttt gcagaaatta
     1141 aacccggcga agcttttgag ttttataacg accacgaccc tcttccactt tattatcaaa
     1201 tggaagcaga aagcaaagac ccattcaaat gggaatatat tattaaaggg ccagaggaat
     1261 ggaaagttag agtaataaaa attaaacctt aattacttac taacaatagt tgaagaatag
     1321 tttgataaaa taattttatc aaactatttt tatttacaaa tttcatcata aacatagcct
     1381 gccctttgta aggctctaat tgccgattct agtgcaattg gtgcaggatt tggtggatga
     1441 agaggcactt cttcggtagc aataagttgt tgtaataagg tatttgtttg tgctatggtt
     1501 aaagcttcac aaatatcata taaaccacca tctttctctt cttcaaaatc atgcttattc
     1561 aacacaaatt gaattgcatt aataatagct tttgttggtg gtggtactaa taaagctgtt
     1621 aatgccccat gttccaatct aaattgtgga attatttttt gaaccaattc tttatttact
     1681 ttggctgcag caggaaaaag tattttctct tccattttaa tatgcctaag caaacctttt
     1741 ctaaataaat ggtaatactc tacattaatc tcattgtctg cttcaataac atttaccaaa
     1801 tatccttcta acctatgatg atccttggta aaaaaatcgt gtaatggttt attcatataa
     1861 aaaatatcaa taataaaaac atgtgaaaat taagccaaca ttaaataaaa taaagcaatt
     1921 atgaacctat aaagatgttg tcatccttta tatctataaa tttgcaacgc taaaattatt
     1981 gattattaaa ttaatggcaa aaatggcaaa accaattata tattcttgct ctggttgctc
     2041 tagtgctgca caaatggcca actacatggc agtaaaatta gacagaaaag atgtagctga
     2101 aatgtcttgc attgtaggtg ttggaggtaa tgtaaaaaaa ttagtaaaga cagctaaatc
     2161 tgggcgaaaa ataattgtga ttgatgggtg tccattaaac tgctctaaag cttgtttaaa
     2221 caatcagggt attgaaccag atttacattt tgagctaaca aaatttggtg taaaaaaaca
     2281 aaaccatata gattttgata tggaacaagc aaataactta ttagaattaa tagaaaataa
     2341 aattgatgaa gcaatagtaa aagaaacaag cttagtgttg aattaaaaat gaaaaataaa
     2401 atagaattat tagcccctgt aggctctttt gaaagtttac atgctgcaat acaggcaggt
     2461 gcaaatgcta tatactttgg tgtagagcaa ttaaatatgc gtgccaaatc aattaactct
     2521 tttatgcttg atgatttagc agccatacaa gcattatgta ttccaaaaaa cattagtacc
     2581 tatattactt taaatacagt aatgtatgaa cacgatatgc agttgttaaa agcaatttta
     2641 aaagaagtaa aaaagcataa aataacagca gtaattgcat cagatttttc ggttatggag
     2701 tattgtaaac aactccaaat tccattacac atttcaacac aagctaatgt tagtaatatt
     2761 gaatcggtaa aattcttttc tactttttct gatgcagtag ttttagctag agagcttaca
     2821 cttaaacagg taaaacaaat aaccgatgaa ataaaaagga agaaaattaa aggtgtttct
     2881 ggccaattaa tgcgtatcga aatttttata catggtgcat tgtgtatggc aatttcgggt
     2941 aaatgttatt taagtttaca tagccaaaac gcatcggcca atcgtggtgc ttgcacacaa
     3001 aattgcaggc atgcctataa agttacagat atagaaacta atgaagaatt agtaattgat
     3061 aatgagtata tcatgtctcc aaaagacctt tgcacaatag atattataga ccagataata
     3121 gaaagtggtg taagtatttt aaaaatagaa ggaaggggca aaggtccaga atatgtatat
     3181 acagttacca aaacctatca agaagcaata acagcagttg aggaaaaaga atatacaaaa
     3241 gaaaaaatag ataaatggaa aacagaacta gaaaaagttt ataaccgtgg cttttgggaa
     3301 gggtattatt taggaagaaa attaggagaa tggacattaa acccaggctc aatggctaca
     3361 gaaaaaaaag tatatatagg aaaagggaaa aaatattttt ccaaaattag tgtaggcgaa
     3421 tttgttgtag aaaccggaaa agttgaaatt ggagacacca tcattattac aagcccaaat
     3481 tttggaattg taaaagaaaa tatagagcaa cttacagtta atggcaataa tgcaactgtt
     3541 gctgttaaag gagatagcat aaccttacgt tgcaaaaata aaatttcggc taaagataaa
     3601 atttacaaat tagtacaaag ttaaatgccc aatattattc attatagaag caaatgtatt
     3661 ggttgcaata tttgttatga aatgatgcca tccacttggc gaatgtctaa aaaagatggt
     3721 aaagcaacat tactaaaagc agagcaaaaa aggcaaacca gtgttttaca aatagatacc
     3781 aacttgatag aggaagcaaa aaaagtagca aacgcctgcc ctgtaaaaat tattaaagta
     3841 aacggttaac aattaccaat aaaaacattt cccaatttgg tttcttctgc tagtttactt
     3901 attggttcat tagtaaataa atttactaat tgttctttaa tatccttata tctactatgc
     3961 attgggcatg gttttttttc ggaacatttg tttaaaccta atacacattt ctctaaaact
     4021 atactttcgc cggtagcttc taatactgaa cgtacaggta aattcttagc ttcatctgtc
     4081 atataaaatc caccttttgg gccacttacc gaactaatta ctctactatc tttggtaagt
     4141 aattgtaaaa cttttgcagt aaaagattta ggagaatcta ttgcttttgc aatttcatct
     4201 atacctattt tatttactac cgagctattt tgggcaatat aaattgttgc ccttaatgca
     4261 tactccgttg ttttagaaaa catataatta gcctgttcta aagttttgaa taattaaagt
     4321 aaaaatatat ttttttaaaa cagactaaca aaataaaagc atttacccat tcatgccaat
     4381 aattataaaa tatttttttg aaaaagtatt cagattatgt agtctttatt tatatttaaa
     4441 aagtaattta taaacttctt atttaaagca atttgtttca tttatgtgtg agtgagatag
     4501 cggagctgta aaaacttgta aatgtcccat aatattttac caatcattaa aataggtggt
     4561 gtcaatcatc acaataaaga gtggattgca atttattgta aaaaagattt taccattaat
     4621 agtattatta aaaaaataaa aggaaacaca tttagtgcaa gtaagcaatg ttggttaata
     4681 ccttatgaaa aatataatta cgattattta gtaaagcatt taaaagagct tgccagtata
     4741 gatgataaat taatatccat atttaaaaaa gagaaagaaa aaaaattaac tggtataaca
     4801 aataaattaa gtaaccaaac attattacaa atatcaatac agttacccaa tcagcaaaaa
     4861 atacaccctg ttaataagca agttttagaa caagtgcaac agcatttgca attaaaagct
     4921 tatagcacaa gtacaagaaa aacatattta aatgaaatag cagccttttt agcaacaata
     4981 aaacatgtca aagcatctgc ccttacacca aatagaatta aagattattt acagtattgt
     5041 tacaaagaat taaagctttc agaaaatacc atgcatagta gaataaatgc attaaaattt
     5101 tattacgagc aggtactaaa aaaagataaa tttttttacg aaatacccag ggcaaaaaaa
     5161 ccctacaagc taccaaatgt tttatccgaa aaagagattg ttagattatt taacacacta
     5221 caaaatcaaa aacataaagc aatattattt atagcatata gtgctggcat gagagtaagt
     5281 gaagttgtag ggttgaaaat tagcaatata gatagtcaaa gaatgcaaat aaaaatagaa
     5341 ggagccaaag gcaaaaaaga taggtatgta aacttaagta ctatagtttt agaagtttta
     5401 agaagctata taaagcaatg cgtacctcgt ccaaaaaaat acttatttga aagtgaaata
     5461 gcaggcgaac cctattcatc aagatcggca caaaaagttt ttcaaagagc aaaagaaaaa
     5521 gctggtatta gtaaaaatgt aacctttcat tcattaaggc acagctttgc aactcatttg
     5581 ttggaaaaag gcgtagatat aagatatata caagatattt tagggcataa taatataaaa
     5641 acaaccgaac gatataccca tgtaaaaaag gaacaaataa tacaaattac aagcccatta
     5701 gatgatttat ggttaaaagg cgacatcaaa atataacacc aaaggcgaca tattttcttc
     5761 ttaaaaaaca aattaacctg tacaaaatca gtacttattt aactttatcg aatataatta
     5821 gttcgtatat ttgagagttg tgtgcaaccc taaaaaacaa cacgaccgac aataaacgag
     5881 cagaaaaaag acaaacaaaa tgccaaccct tcgcaaaata aaaagagctg caagccaacg
     5941 cacaagccga cacaaattgc agcacatttt attttgccca acccacccaa gcccacccac
     6001 cacccaaacc aaagttcagg tcgttttgtt aataactttt ttttaaaaaa attccgcaag
     6061 taatttttct tacttacatt tggaaaatat ttgaccagaa caaattatga ccaaaaagaa
     6121 attgaccaca tttggcgaaa caataagaca acttcgtgag gagcaaaagc tccctcaaag
     6181 aaaggttgca gcaattttgg atattgacac ctcactactt gccaagtatg aaagaaacgt
     6241 aagacaacct tcaaaagaac tgataaacaa gattgccgaa attttcaaag ttgacagtga
     6301 ggaattaata aaagaggcat tgacagacaa aattgcttat caaataattg aagaggatgc
     6361 tgacagcaaa atattaagag ttgcagaaga taaagttgaa tacttaaaaa caaaaaaaac
     6421 aaaatgattg aagatataaa ctatgctctt gttgaggatg tgagaccccc aatctataca
     6481 gcaatgaagt attggggaaa gaaacctcac aatatttggc gaaaatatat tgagaattac
     6541 acccccgaaa acggactttt ccttgacccc ttttcaggaa gtgctattag tgcttttgag
     6601 gcaattaaag caaacagaaa agtaattgca tttgacttaa atccactaac atcttttatt
     6661 atagaaattt ttgctactga ctttgacaaa aataaatttg agaaagaagt caaaagaatt
     6721 tctgacacat tcaaaacaga tgaaatttac aacaaatact ttacaaccca atcaagaaaa
     6781 agcaacgaaa ctacaattgt tcagagtttc aaatgggaag aggataaaat ttatgagtta
     6841 ggaattgaag caacagaaaa ggaaattaaa gagcaaaatg atttaaaaaa gaaaaagaaa
     6901 attgagaaag taattaaacg ttacacttca agacctaata cagaggataa tttaaaagga
     6961 atttcaatga atgaaattca aattccattt tggtttcccg ataagccatt tcataattca
     7021 ccttccttcc ctgcaagctt tattagttgt ttaggcggta ataattttac aaacctttgg
     7081 acaaaaagaa atctttatgt tc
//
LOCUS       DAOQPK010000101         7057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_6020_length_7057_cov_4.844330, whole genome shotgun sequence.
ACCESSION   DAOQPK010000101 DAOQPK010000000
VERSION     DAOQPK010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7057
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6020_length_7057_cov_4.844330"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4427
                     /locus_tag="PLU36_06135"
     CDS             <1..4427
                     /locus_tag="PLU36_06135"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HMZ46365.1"
                     /translation="FIVDHFSSPTKSTSTKSNKDGFALNLKKIDFKNIRFVNNDRWTG
                     EQIDVKLGSFVVDADNIDFNKGSLILNSIIIDKPVVIIDNYTALRPPKLKKPRKEDSI
                     SRLNPADMFVKVTNLKINSGTFISNANQDKPTLHFDGEHIAFNKINGSFNNVLLQKDT
                     ITASIEINCNERSGLVVKQLNAKYKVTPQIMEFANLSLETNKSKLGNYYAMKFNRFNN
                     DFGNYISKVVMVADFRQSNVHSNDIAFFAPALSHINKNIQIGGKYKGTVSDFNITHLF
                     AKAGSSIITGNLSMKGLPDFEKTFISLNNGAVYTTANDLLQYIPILKSYTNPNIPALG
                     NIVYKGSFNGHYNNFISNGNLSTNLGALYTNLSMKFPAKQEPTYTGQINTQQFNLGKF
                     INSPIIGNISFQGKINGSSYTLSKMKTTFDGDIKKLELNGYSYSNISTLGTFEKQAFN
                     GQVKIADPNLDFIGIMQIDLTEDKPKFNLFADILKSNYKELKITPNYNLQVTGTLDVN
                     FKGTNIDNFEGEAKLLNAVVTNNNQLIKFDSLVIASNYINNIKHLRLSSTDFSSNILG
                     SFSIMGLPASFQSFLHKYYPAYIAEPDTIPQNQDFSFDITTGYAEPYLKLIDTNLYGF
                     NDAYITGSINTKANTLQTNIQIPGIGYKDYFLSGVELIGKGTMDSITVSGDILSTVIN
                     DSTHLPMTKFSISSANDHSKVNIQTRANSTLNDAFISADVFTLEDGARVEFQPSSFVI
                     NEKKWNIEKNGIFVVRKNFLSTNNLKLTQGLQEIYFNTETEDDNNATNLIIDFKNVVI
                     GDFVSYALKDPKLEGVTTGKIKVDNIFRNLTADANLQVKHFKVDEDSIGIANVIAGYN
                     SKTGKITWNWISPNTDYDFNIKGSYDTKDSTVTNKLNTDIVFNKTRIKPLQKYLKGIF
                     SDVDGIATGTLKVVGNDSVNLIGNVLVKNAGLLVDYTQVYYIIDSAQIKFEEDGIDFG
                     RFTVKDKFNNQALVKGKLYEKNFKNMAFDFEANTNKLLLLNTKAKDNNQFFGTAIGKA
                     SLSFKGPETNAKMTIIASSNDTSHIYIPNSVSKESDEADFIVFKQFGKEMEVQKKSSA
                     FNLLVDLDLTANEKVSIDVILDDIAGDVIKATGNGRLHIKAGTNEKLDIRGRYNIENG
                     LYDFNFQSLIKKPFILMPDVGNYIEWSGDAMDAKIHVDASYETNNVRLSDLTGNASGI
                     MSSNVSNLRESVFVIAQLRDQLMQPSIKFKIDFPQNSPVKTDPDFAQFLGRMEKDENE
                     MLQQATSLIVFGSFAPYGKGLLAGGNSYTSNISGYVNSLSQRIMSAASKIISGFLAKL
                     FNDKSLKVDLGTSIYSSGSILNQGVNTTTSKVDRQIFNFKIGKSFFNNNVIVTFGSDL
                     DFNIGNAASGDLQWLPDLNVEFVLSKDRKLRAIIFNKNSLDISGATFGRRNRQGISFS
                     YRQDFERFFSKASNQQSPTSSKPTTSLSKDVEFIRKDP"
     gene            4500..5888
                     /locus_tag="PLU36_06140"
     CDS             4500..5888
                     /locus_tag="PLU36_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46366.1"
                     /translation="MALYNNIYNLSPVWLQNVLVSAYGYNWKKKRYGGIFNKEYTAAK
                     QRENFTIQQWHNYQNEQLTKILLHAYNNVPYYKNKFTKLGLTITDLKQVNIDTLDKLP
                     FLLKEDVRQFGTTILMSNSREEGGSFFASSGSTGTPIQVCYSNNMHQRWFALNEARIK
                     NWAGVNKEMSRGMIGGRRVVPDADNNPPFYRYNFFEKQVYFSAYHISPVNINNYAKAF
                     NKYSIDYMTGYAMSNYILARMFLENNIQIPPLKAVLTSSEKLTPTMRETISKAYNCKV
                     FDAWSGMEYCSLISECEHGNLHISPDAGIIELLDNNLQPVKVGEIGNVYCTGFLNFDQ
                     PLIRYKIGDQMILRNTTCGCGRSMPVIDEIIGRVEDVVYGKDGREMVRFHGIFVGLKT
                     VKLAQVIQNSLNEITIKLVPDKALADDEKELIIKRVKSQLGDIDIIIEEVMDIPKTAN
                     GKIKAVISTIKK"
     gene            complement(5897..>7057)
                     /locus_tag="PLU36_06145"
     CDS             complement(5897..>7057)
                     /locus_tag="PLU36_06145"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HMZ46367.1"
                     /translation="FYRNNKYSDSVQYAALVISKTAPPKFIQLGTLAKLEHFMQGKKT
                     DSKELNIHRLYRSRLAKKEASFYGDSIYNFLWKPLEEYIKGSTNIAYAPDGILHKLAF
                     QALPVDSNKILLDKFQLQQYSSILQIAERKKENVFNWQSALLFGGINFNEAKVKNKTT
                     INYQSSSAAYAALPATINEISGIEKIFLKNKLTITTVSGSKATEEFFKSINNHSPQIL
                     HIATHGYFLKEVVNTNSTNLMLRGEQLISTSENSLLRSGLVLAGANKAWSGEKLPDSL
                     EDGILSAYEISKLNLSNTKLVVLSACETALGDINGTEGVFGLQRAFKIAGAKNLIVSL
                     WQVPDKETAELMQLFYTNLMQGNSIRNSFYKAQKEMRSKHAPFAWAAFILIE"
BASE COUNT         2614 a          978 c         1151 g         2314 t
ORIGIN      
        1 agtttatagt tgaccatttt tcttctccta caaaatctac aagtactaaa agtaataaag
       61 atgggtttgc tttaaactta aaaaaaattg attttaaaaa tataagattt gtaaacaatg
      121 acagatggac tggtgagcaa atagatgtaa aacttggcag ttttgtggtg gatgcagata
      181 atattgattt taacaaaggc tcgttaattt taaattctat aataattgat aaacctgttg
      241 taataattga taattacact gcattaagac cacctaagct taagaaacct agaaaggaag
      301 atagcatatc aaggttaaac ccagcagata tgtttgtaaa agttaccaac ctaaaaatta
      361 acagtggtac atttataagc aacgccaatc aagataagcc tacactacat tttgatggag
      421 aacatattgc ttttaataaa attaatggca gttttaataa cgtgttgcta caaaaagata
      481 caattactgc aagtattgaa attaactgta atgaaagaag tggcttagta gttaaacagc
      541 taaatgctaa gtataaagtt accccacaaa taatggagtt tgccaattta agtttagaaa
      601 ccaataaaag taaattgggt aattactatg caatgaagtt taataggttt aacaatgatt
      661 ttggtaatta tatctctaaa gttgtgatgg ttgcagattt taggcagtcc aatgttcaca
      721 gtaatgatat tgcatttttt gcaccagcct tatctcatat aaataaaaat atacaaatag
      781 gtggtaaata caaaggcact gtatcagatt ttaatataac tcatttattt gccaaagcag
      841 gttctagtat aattacaggc aacttaagca tgaagggatt gcctgatttt gaaaaaactt
      901 ttattagttt aaataatggg gcggtttata ccactgcaaa tgatctttta caatacatac
      961 ctattttaaa atcctatact aatccaaata taccagcact aggaaatatt gtttataaag
     1021 gaagttttaa tgggcattat aataatttta tttccaatgg taatttaagc accaatttag
     1081 gtgcacttta caccaatctt tctatgaaat ttcctgcaaa acaagagcct acttatactg
     1141 ggcaaattaa tacgcagcaa tttaatttag gaaaatttat taactcacct atcatcggca
     1201 atatctcttt tcaaggtaaa ataaatggca gtagttacac attaagtaaa atgaaaacta
     1261 catttgatgg agatattaaa aaattagaat taaatggata tagttattcc aatatttcaa
     1321 cattaggaac ttttgagaaa caagccttta acggacaagt gaaaattgca gatcctaatt
     1381 tagattttat tggcattatg caaatagatt taacagaaga taagccaaaa tttaacttgt
     1441 ttgcggatat tttaaaatct aattataaag aactaaaaat tactccaaat tacaacctgc
     1501 aagtaacagg cacattagac gtgaatttta aaggtactaa cattgataat tttgaaggtg
     1561 aagcaaaact tttaaatgca gttgtaacaa acaataatca attaattaaa ttcgattctt
     1621 tggttattgc ttctaactat atcaataata taaaacattt acgattaagt agtaccgatt
     1681 tttcaagtaa tattttaggc agctttagta ttatgggttt gcctgcaagt tttcaatcat
     1741 ttttacataa gtattaccct gcttatattg cagaaccaga taccattcca caaaatcaag
     1801 attttagttt tgatataact actggttatg ctgaaccata tttaaagtta atagatacta
     1861 atttatatgg ttttaatgat gcatatatta caggaagcat aaatacaaag gcaaatacac
     1921 ttcaaacaaa tattcaaata ccaggtattg gttataaaga ttatttttta tctggtgtag
     1981 aattaatagg caaaggaact atggactcca taacggtttc aggagatatt ttaagcactg
     2041 tgattaatga tagcactcat ttaccaatga ctaagtttag cataagctcg gctaacgatc
     2101 attcaaaagt taatatacaa acaagagcaa acagtacttt aaatgatgca tttatttcgg
     2161 ccgatgtttt tactttagaa gatggtgcaa gagtagaatt tcaaccttct tcatttgtga
     2221 ttaatgaaaa aaagtggaat attgaaaaaa atggaatttt tgttgttcga aaaaatttct
     2281 tatcaactaa taatttaaaa cttacacaag ggttacaaga aatatatttt aatacagaaa
     2341 cagaagatga taacaatgca actaatttaa ttattgattt taaaaatgta gttattggag
     2401 attttgttag ctatgcttta aaagacccaa aattagaagg agttactaca ggaaaaatta
     2461 aggtagataa tatttttaga aacctaacag ccgatgccaa tttacaagta aaacatttta
     2521 aggtagatga agactccatt ggaattgcaa atgttatagc aggatacaac agtaaaacag
     2581 gtaaaattac ctggaactgg atttctccta atacagatta cgattttaac ataaagggta
     2641 gttatgatac taaagactct acagttacta ataaattgaa tacagatatt gtttttaata
     2701 aaacaagaat taagccctta caaaaatatc tcaaaggaat tttttctgat gtagatggaa
     2761 ttgcaacagg tacattgaaa gtagttggaa acgattctgt aaacttaata ggtaacgtgt
     2821 tggtaaaaaa tgcagggcta ttggttgact atacgcaagt ttactacatc atagattctg
     2881 ctcaaataaa gtttgaagaa gatgggatag attttggaag atttactgtt aaagataaat
     2941 ttaataatca ggcattagtt aaaggaaaac tatacgaaaa gaattttaaa aatatggctt
     3001 tcgattttga agccaacacc aataaactat tattattaaa tactaaagct aaagataata
     3061 atcaattttt tggaacggca attggcaaag cctcattaag ttttaaaggg ccagaaacca
     3121 atgctaaaat gaccattatt gctagctcta acgatacttc acacatttat atacccaata
     3181 gtgtaagcaa agaaagtgat gaagctgatt ttattgtatt taaacagttt ggtaaagaaa
     3241 tggaagtgca aaaaaaatca tctgcattta atttgctggt agatttagat ttaacagcca
     3301 atgaaaaagt atctattgat gtaattttag atgatattgc tggagatgta attaaagcaa
     3361 caggtaacgg aagattacac attaaagctg gaaccaacga aaaactggat attagaggaa
     3421 gatacaatat tgaaaatggt ttatatgact tcaactttca atctttaatt aaaaagccat
     3481 ttattttaat gccagatgtt ggcaattata ttgaatggag tggtgatgca atggatgcta
     3541 aaattcatgt agatgcatct tacgaaacaa acaatgttcg tttatccgac cttactggca
     3601 atgcttctgg cataatgagt agcaatgtaa gcaatttaag agaaagcgtt tttgtaatag
     3661 cccaattaag agatcaactc atgcagcctt ctattaaatt taaaatagat tttcctcaaa
     3721 acagccccgt taaaaccgat cctgattttg cacaattctt aggcagaatg gaaaaagacg
     3781 aaaacgaaat gttgcaacaa gcaacttcct taatagtatt tggctctttt gcaccttatg
     3841 gcaaaggctt attggctggt ggtaatagtt acacttccaa tatttctggg tatgtaaata
     3901 gtttatcaca aagaattatg tcggcagctt ctaaaattat ctcaggattt ttagcaaagc
     3961 tatttaatga taaaagttta aaagtagatt taggaacttc aatttatagt agtggtagta
     4021 tattaaacca aggtgtaaat acaactacca gtaaggtaga taggcaaata tttaatttta
     4081 aaattggtaa aagtttcttc aataataatg taattgtaac ctttggcagc gatttagatt
     4141 ttaatattgg taatgcagct tcaggagatt tacaatggtt gccagatttg aatgttgagt
     4201 ttgtattatc aaaagacaga aaattaagag ccatcatttt taataaaaat agtttagata
     4261 ttagtggtgc tacttttggc agaagaaaca ggcaaggtat tagttttagt tatcgccaag
     4321 attttgaacg tttttttagc aaagccagca atcaacaatc accaacctct tcaaaaccaa
     4381 caacctcatt aagtaaagat gttgaattta taagaaaaga tccttaaaca attttcatta
     4441 cttttatttc gttctagaac aaatttttca atacattatt ctcttttaaa ttttttagag
     4501 tggcattata caataatatt tacaatctaa gccctgtatg gttgcaaaat gtattagttt
     4561 ctgcttatgg gtataattgg aaaaagaaaa ggtatggagg aatatttaat aaggaatata
     4621 ctgctgcaaa acaaagagaa aattttacaa ttcaacaatg gcataattat caaaatgagc
     4681 agctaacaaa aattttatta catgcctata ataatgtgcc ctattataaa aataagttta
     4741 caaaattggg gcttacaata acagatttaa aacaagtaaa tattgataca ttagacaaat
     4801 tacctttttt gttgaaagaa gatgttaggc agtttggtac tacaatattg atgagtaata
     4861 gtagagaaga aggaggtagt ttttttgctt ctagtggaag tacaggaact ccaatacaag
     4921 tttgttattc aaacaatatg caccaacgct ggtttgcatt gaacgaagct agaataaaaa
     4981 attgggcagg cgtaaataaa gaaatgagta gaggtatgat tggtggtaga agggtagtgc
     5041 cagatgctga taataatcct cctttttaca gatacaactt ttttgaaaaa caagtttatt
     5101 tttctgccta tcatattagc cctgtaaata taaacaacta tgctaaggct tttaataaat
     5161 attcaataga ttatatgaca ggctatgcca tgagtaatta tattttggca agaatgtttt
     5221 tagaaaataa tattcaaata ccaccattaa aagctgtgct tacctctagc gaaaagctta
     5281 cacctacaat gagagaaacc ataagcaagg cttataactg taaagttttt gatgcatgga
     5341 gtggcatgga atattgtagc ctaatttcag aatgtgaaca tggtaatttg catattagtc
     5401 cagatgctgg cattattgaa cttttagata ataatttaca accagtaaaa gtaggagaga
     5461 taggaaatgt ttactgtaca ggttttttaa attttgatca accacttatt agatacaaaa
     5521 ttggcgacca aatgatttta aggaatacaa cttgtggttg tggtagaagc atgccagtga
     5581 tagatgaaat tattggtaga gtagaagacg ttgtgtatgg aaaagatgga agagaaatgg
     5641 ttcgttttca tggaattttt gttggactta aaacagtaaa gctagcacag gtaatacaaa
     5701 acagtttaaa cgaaataact ataaaacttg tgccagataa agctttggca gatgatgaaa
     5761 aggaattaat tattaaaaga gtaaaaagcc agttaggtga tattgacata attattgagg
     5821 aagtaatgga tattcctaaa acagctaatg gaaaaattaa agctgttatt tccaccatta
     5881 aaaaataaat ttttcattat tcaattaaaa taaaagcagc ccaagcaaaa ggtgcatgtt
     5941 tgcttctcat ttctttttga gctttataaa aagaatttct gatactatta ccttgcatta
     6001 agttggtata aaataattgc attaactcag cagtttcctt atcgggcact tgccataaac
     6061 ttacaattaa atttttagca cctgcaattt taaaggcacg ttgcaaacca aaaactccct
     6121 cggtaccatt aatatcacct aaggcagttt cgcaagcact tagcacaaca agtttagtgt
     6181 tagataagtt tagtttgcta atttcataag cactcaaaat tccatcttct aaactatctg
     6241 gcagcttttc tccactccat gctttgttag cacctgctaa tactaaacca cttctaagca
     6301 gcgaattttc tgaagtagag attaattgct ctcctcgtaa cattaaatta gtagaattag
     6361 tattaacaac ttcttttaag aagtaaccat gtgttgcaat atgtaatatt tgaggtgagt
     6421 gattgtttat agatttaaaa aattcttccg ttgctttact accactaaca gtagtaatag
     6481 ttaatttatt ttttaagaat attttctcaa ttcccgaaat ttcatttatt gtagcaggca
     6541 aagctgcata agctgcagaa gatgattggt agttgattgt agttttattc ttcacttttg
     6601 cttcattaaa attaattccg ccaaatagta atgccgattg ccaattaaat acattttcct
     6661 tttttctttc tgcaatttgc aaaatgctac tatactgttg caactgaaac ttatctaaca
     6721 aaattttatt actatccact ggcaatgcct gaaaagctaa tttatgcaaa atgccatctg
     6781 gtgcataagc aatattggta ctacctttta tatattcttc taatggcttc cataaaaaat
     6841 tgtaaatact atcgccataa aatgaggcct ctttttttgc caatctgctt ctatataagc
     6901 gatgaatatt tagctcttta gaatctgttt tttttccctg cataaaatgt tcaagctttg
     6961 caagcgttcc taattgtata aacttaggag gggctgtttt acttataact aaggcagcat
     7021 actgaacaga atctgaatat ttgttgtttc ggtaaaa
//
LOCUS       DAOQPK010000102         7045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_6040_length_7045_cov_3.972532, whole genome shotgun sequence.
ACCESSION   DAOQPK010000102 DAOQPK010000000
VERSION     DAOQPK010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7045
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6040_length_7045_cov_3.972532"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..286
                     /locus_tag="PLU36_06150"
     CDS             26..286
                     /locus_tag="PLU36_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HMZ46368.1"
                     /translation="MDIKQLFELAVTNSKTLTEKPNNEILLQLYSLYKQATEGDNKED
                     APSNPFDFVAKFKHEAWTKLKGLSKETAMQQYIDLVNNLKNG"
     gene            420..3071
                     /locus_tag="PLU36_06155"
     CDS             420..3071
                     /locus_tag="PLU36_06155"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ46369.1"
                     /translation="MLKKSLLIILAVFQLTSLFAQALYPVSIQEKAANSTLIIEGKVV
                     ERTSFWNTAHTMIFTSNKIKIYKVFKGEVAADFVEVLTQGGSVGNDAIHASDLLDLVW
                     GQEGIFFCHPNVINLKSPNTNNSLLEVYSSSQGFLNYNILEDKATDPFNVYNGISTNL
                     YPLIMQLTGQSFVIKDNTFNVNQQKQNLANKTSAPSISSFTPETVNAGAMLDPTNNLL
                     TINGTGFGTFTGNAAVIFNDANTIAGTDYLVAASNPQLSNLVVSWTDTEIKIKVPARA
                     GSGAIKVRDNLGNVAYSFTNLTAHYGVINTTFNSGGTYYVKEFNLMDVNSAGGYTVYY
                     STSTSGGGVNLDISPTKQTFQRALTTWREISGFNVIEGGTTTTQVITGSTSECVIMYD
                     NTNTGVSPLSAGTLAVCYSYSGICNVTNEARKPKFDVIIRNNGVSSGTTNFNEGPCPS
                     IAVNNSYIDLESVLLHELGHALNLAHVNLPSEGAGAGTTNPGNIMHYSVHGSNRRITP
                     DKSARDGATYSIQQQNNVYGNCTSQTEMVPLTIITEPKDECPLTFPSTPTDGGTIVNF
                     DMVHATSNRFVDPYFTQIKCDGSGSNVTSTAFYPILTDNSGNLNISITNYIATPTSLT
                     LCSSVIPGQLGTGFRFALYEVSTCPSAGAYPTPISCTTFTKNMVFNPITGLSANTNYL
                     IFISAIENTKANFNMVISGSALPIKLSSFNGKVQRDYNNLDWTADQIINVNKIEIERS
                     STGSDFTTIGTIDGAAVYNKVGNFKDYKPTINSYYRLKIINNDGSVEYSKTINLKRTE
                     KVLFSISPNPATTYADVQISSEVKGKYSLVLYNLNGQRVYSKAINLNQGLNNHKINTV
                     TFAKGVYRLILMNENNNNVYSNSLIVQ"
     gene            complement(3221..4315)
                     /locus_tag="PLU36_06160"
     CDS             complement(3221..4315)
                     /locus_tag="PLU36_06160"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HMZ46370.1"
                     /translation="MIIISCNNNNKSKVNNHLFPFRGLSKIEKLDFSSKALNIYNSIL
                     GNEFSGGILIAKNGEIVFEKYNGFINFKEKEHIKSSTPIHIASISKPITALAILQLVE
                     KKLISLDDTIQKFFPSFPYQNITVLHLLNHRSGLPNYLYFMDTAWRASTKFVTNNDVL
                     QFMIKNQPAAYSNPNTVFHYCNTNYVLLAIIVESVTHQFFPNYIKENIFKPIGMQNSF
                     VFNINDTSNYVPSYQFNNIPYTLESFDCVYGDKNVYSTVRDLWLLDKALYNNSIISKN
                     TFNIATTAYSNETKGNKNYGLGWRLFLKNNDTVIYHGGWWHGNNSLFTRLIKDTATVI
                     TLGNRYNKRIYNSKQMATIFSNKEIGEVEE"
     gene            4441..5682
                     /gene="serA"
                     /locus_tag="PLU36_06165"
     CDS             4441..5682
                     /gene="serA"
                     /locus_tag="PLU36_06165"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HMZ46371.1"
                     /translation="MNKKLQNTSYPKEKINILFLENISEKAVQILKQHGYVNVKKNSG
                     ALSEEELIKAIKDVHVLGIRSKTFITEKVLQAANKLQAIGCFCIGVNQVNLQAATNKG
                     VVVFNAPYSNTRSVAELVIGSAIVLIRKIIDKNNAAHKGIWLKDSKGSYELRGKTLGI
                     IGYGNIGSQVSVLAESLGMKVMFYDVETKLPLGNATVCKNLKELLSVSDIISLHVPET
                     PQTKNLINKNNLKYFKNGCILINYARGEVVDLDALATALKEMQLSGAAVDVFPVEPEK
                     NGDTFKSPIQGLNNVLLTPHIGGSTEEAQQNIGEDISYKLIHFLESGITNGSHTVPAI
                     ILPPVDSAHRILHIHKNVPGVLSAINTSLSKHNINIVGQHLKTNDDIGYVVLDVDKKL
                     SKHAVELLKEIKETIKVRMLY"
     gene            complement(5679..6737)
                     /locus_tag="PLU36_06170"
     CDS             complement(5679..6737)
                     /locus_tag="PLU36_06170"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HMZ46372.1"
                     /translation="MKNKILFSLIFTLYLSNVFAQKKGVQILNTGAPIQVSFRGMSVA
                     DENTVWISGNNGTVGKTTDAGKTWQWTRVKNFEQNDFRDIHAFDSNNAIIMAVGNPAY
                     ILKTKDGGKSWYTVFSRNMEGMFLDAMDFLDNTTGVCIGDPISVGNSGRRFFFIIKTI
                     DGGETWEQEPLYKMPPAQEAGEAIFAASGTNIAMLQHPDFEYAFATGGTVSNIYFIGR
                     EGKQNKGAQIPINQGIETTGTFSLATDGKKKFYCIGGDYKLPNSTYDNFYWSTDVGKK
                     WASPNTAPPYGYRSCLRIIDKETMIACGLNGVDISKDGGDDWKNISKEGFNVCMVSAK
                     NKMVFLAGDRGKVGIYYY"
BASE COUNT         2554 a         1082 c         1022 g         2387 t
ORIGIN      
        1 atcatcatta caataataaa acaatatgga tataaaacaa ttatttgaat tagctgttac
       61 taacagtaaa actttgacag aaaaacccaa caacgaaatt ttattacaac tttatagcct
      121 atataaacag gcaacagaag gagataataa ggaagatgca ccatctaacc cattcgattt
      181 tgttgctaaa tttaaacacg aagcttggac aaaattaaag ggactaagta aagaaactgc
      241 tatgcaacaa tatatagatt tggtaaacaa tttaaaaaat gggtaaatac aatactttat
      301 aatgcagcat aaaaagctat tcaagtatca aaatactagc tttattacta gttagtaact
      361 aattacaata tggatactaa attttacaaa ttattttatc tttgctacaa attacattta
      421 tgttaaaaaa atctttactc attattcttg cagtttttca actaacaagt ttatttgctc
      481 aggcacttta tcctgtatca attcaagaaa aagctgccaa ttctacctta attattgaag
      541 gtaaagtagt agaaagaact tctttttgga atacggcaca tactatgata tttacttcca
      601 ataaaataaa aatttacaaa gtttttaaag gagaagtagc tgccgatttt gtagaagtat
      661 taactcaggg tggttcagta ggcaacgatg ccattcatgc aagtgattta ttagacttag
      721 tttgggggca agagggtata tttttctgcc atcccaatgt tattaattta aaatctccta
      781 atacaaataa tagtttatta gaagtttatt caagctcaca agggttttta aactataata
      841 ttttagaaga caaagcaacc gacccattta atgtatataa tggaatatct accaacttat
      901 atccattaat tatgcaacta acaggtcaat cttttgttat taaagacaat acatttaatg
      961 taaatcagca aaaacaaaat ttagccaata aaacatctgc accatctata agttcattta
     1021 caccagaaac tgtaaatgca ggtgcaatgt tagatcctac aaacaactta ttaaccatta
     1081 acggtactgg ctttggaact tttactggaa atgctgctgt tatctttaac gatgcaaaca
     1141 caattgccgg aactgattat ttagttgctg cttctaaccc acaactttcc aacttagtag
     1201 tttcctggac cgatactgaa attaaaatta aagtacctgc aagagctggt agtggtgcaa
     1261 taaaagttag agataatctt ggtaatgttg catactcctt taccaattta actgcacatt
     1321 atggtgtaat taataccact tttaactcag gtggtacata ttatgtaaaa gagtttaatt
     1381 taatggatgt taatagtgcc ggtggttata cagtttatta tagtacaagt acttctggtg
     1441 gaggtgtaaa tttagatatt agcccaacca aacaaacttt tcaacgtgca ttaacaactt
     1501 ggagagaaat ttctggcttt aatgttatag aaggtggtac tacaacaact caagtaatta
     1561 caggttctac atcagaatgt gttattatgt acgataatac caacactggc gtttcacctt
     1621 tatctgcagg tacacttgct gtatgctatt cttactctgg catttgtaat gttactaatg
     1681 aagctagaaa accaaaattt gatgttatta ttagaaataa tggcgtatct tctggaacaa
     1741 ctaattttaa tgaaggacct tgcccttcaa tagcagtaaa taattcttat atagatctag
     1801 aatcagtttt actacatgaa ttaggccatg ctttaaacct tgctcacgtt aaccttcctt
     1861 cagaaggtgc tggtgcaggt acaactaatc ctggtaatat tatgcactat tcagttcatg
     1921 gatccaatag aagaataaca ccagataaat cagctagaga tggtgctaca tactccatac
     1981 aacaacaaaa taatgtatat ggtaattgta cttctcaaac agaaatggta cctctaacta
     2041 taattactga accaaaagat gaatgtcctt taaccttccc ttctacacca acagatggcg
     2101 gtacaattgt aaattttgat atggttcatg caactagtaa cagatttgtt gacccatatt
     2161 ttacccaaat taaatgcgat ggttctggaa gcaatgttac cagtacagca ttttatccta
     2221 ttcttactga taatagtggt aacttaaata tttcaattac taactatatt gcaacaccaa
     2281 cttcattaac tctatgctct tctgttatac ctgggcaact tggtacaggg tttagatttg
     2341 ctttatacga agtaagtact tgtccttcag caggtgcata tccaacccca atttcttgta
     2401 caacttttac aaaaaacatg gtgttcaatc ctataacagg attaagtgct aatacaaatt
     2461 atttaatttt tataagtgct atagaaaata ctaaggctaa ttttaatatg gttattagtg
     2521 gttcagcact tcctataaaa ctaagctcat ttaatggtaa agtacaacgt gattataata
     2581 atttagattg gactgcagac caaattatta atgtaaataa aatagaaatt gaaagaagta
     2641 gcacaggatc agattttaca acaataggca caattgatgg ggctgctgtt tataataaag
     2701 taggaaactt taaagattat aagccaacaa taaatagtta ctatcgttta aaaataatta
     2761 ataatgatgg tagtgtagag tattcaaaaa caattaattt aaaaagaacc gaaaaagtat
     2821 tattttctat ttcaccaaac cctgcaacta cttatgcaga tgtacaaatt tctagcgaag
     2881 taaaaggtaa atactcttta gttttatata acttaaatgg ccaaagagtt tatagtaaag
     2941 caattaactt aaaccaaggt ttaaataacc ataaaattaa tacagtaaca tttgctaaag
     3001 gtgtttatcg tttaatatta atgaatgaaa ataataacaa tgtttattct aattcgttaa
     3061 ttgttcaata agtttttttc aagtatttta aacaggttac taactattta gtaacctgtt
     3121 ttttttgata gtagataaaa ataaaggctt ttaattttta ataaaaaata ctaactaaaa
     3181 agtacattaa gcttatcaat aagtgcaatt ttagttataa ctattcttca acttctccta
     3241 tttctttatt agaaaatata gtagccattt gtttgctatt ataaattctt ttgttatacc
     3301 tattacctaa agtaataaca gtagcagtat ctttaattaa cctagtaaat aaactattat
     3361 taccatgcca ccaaccacca tgataaataa ctgtatcgtt attctttaaa aataaacgcc
     3421 agcctaaacc ataattttta tttcctttag tttcattact ataggcagta gtagctatat
     3481 taaaagtatt tttggagata atactattat tgtacaaagc cttatctaat aaccataaat
     3541 ctctaactgt agaatataca ttcttatctc cataaacaca gtcaaagctc tctaatgtat
     3601 agggtatatt attgaattgg taagaaggaa cataattgga agtatcattt atattaaaca
     3661 caaagctatt ttgcatacca attggtttaa atatattttc tttaatataa ttaggaaaaa
     3721 attgatgtgt aacagattca acaattatag ctaataaaac ataattggtg ttacaataat
     3781 gaaaaacagt gtttgggttt gagtaagcag caggttggtt tttaatcata aattgtaaca
     3841 catcattatt ggttacaaat tttgtagaag ccctccaagc agtatccata aaatataaat
     3901 aatttggtaa gccacttcta tgatttagta aatgaagtac tgttatattt tgataaggaa
     3961 agctagggaa aaatttttga atagtatcgt ctaagctgat tagctttttt tcaacaagct
     4021 gtaatattgc taatgcagta ataggtttag aaatagatgc aatatgaata ggagttgagc
     4081 ttttaatatg ttccttttct ttaaaattta taaagccatt gtatttttca aaaactattt
     4141 ctccattttt ggcaattaaa attcccccac taaattcatt ccctaaaatg ctattataaa
     4201 tgtttaatgc tttactcgaa aagtctaatt tttcgatttt acttaagccc ctaaatggaa
     4261 ataaatggtt gttaactttg cttttattat tattgttaca agatataatt atcatcccca
     4321 atattaatag cactactcta atcataattt taaaaaatat actttgttta aagccaacaa
     4381 atatagttta gcaccaatgt attgttccat aaattgttca caaatcgttt aatttgctgt
     4441 atgaataaaa aattacaaaa tactagttat ccaaaagaaa aaataaatat ccttttttta
     4501 gaaaatatta gcgaaaaggc tgttcaaatt ttaaagcagc atggatatgt taatgtgaaa
     4561 aaaaattctg gagcattaag tgaagaagag ttaattaaag ccattaaaga tgttcatgtt
     4621 ttaggcataa ggtcaaaaac atttattacc gaaaaagtat tacaagctgc aaataaatta
     4681 caagctattg gttgtttttg tattggagta aatcaggtaa atttacaagc agcaaccaat
     4741 aaaggggttg tagtatttaa tgcaccttac agtaatacca gaagtgtggc agaattagta
     4801 attggctctg caattgtatt aattagaaaa attattgata aaaataatgc tgctcataaa
     4861 ggtatttggt taaaagattc taaaggaagt tatgagttaa gaggtaaaac attgggtatt
     4921 attggatatg gtaatattgg tagtcaggta agtgtactgg cagaaagcct tggtatgaaa
     4981 gtaatgtttt acgatgtaga aactaaactt ccattaggta atgcaactgt gtgtaaaaat
     5041 ttaaaagagt tattgtctgt ttccgatatt atttcattgc atgtaccaga aactcctcaa
     5101 accaaaaatt taataaataa aaacaacctt aaatatttta aaaatggatg tatattaata
     5161 aattatgcca gaggcgaagt agtagattta gatgctttag ctacggcatt aaaagaaatg
     5221 caactaagtg gtgcagctgt agatgtattt cctgttgaac ctgaaaaaaa tggcgataca
     5281 tttaaaagcc ctattcaagg cttaaacaat gtactgctaa caccacatat tggaggtagc
     5341 acggaagaag ctcaacaaaa tattggagaa gatatcagtt ataaactcat tcatttctta
     5401 gaaagtggaa ttaccaatgg ctctcacact gtacctgcaa ttattttacc acctgtagat
     5461 tctgcacaca gaattttaca catacacaaa aatgtaccag gtgtattaag tgccataaat
     5521 acatctctat ctaaacataa tataaatatt gtaggacaac accttaaaac caatgatgat
     5581 attggttatg tggtattaga tgtagataaa aaattatcta aacatgcggt tgaattatta
     5641 aaagaaataa aagaaactat taaagttaga atgttgtatt agtagtagta aatcccaact
     5701 tttcctctat cgccagctaa gaaaaccatt ttattttttg cagataccat gcaaacatta
     5761 aaaccttctt tagagatgtt tttccaatca tctccaccat ctttactaat atcaacacca
     5821 tttagcccac aggctatcat ggtttcttta tctattatac gtaaacaact tctgtatcca
     5881 taaggaggtg ctgtattggg agaagcccat tttttcccaa catctgttga ccaataaaaa
     5941 ttatcgtaag tactatttgg taatttgtaa tctccaccaa tacaataaaa cttcttttta
     6001 ccatctgttg ctaatgagaa tgtaccagtt gtttcaatac cttggtttat tggtatttgg
     6061 gcacctttat tttgtttacc ttctctacca ataaaataaa tattacttac tgtaccacca
     6121 gttgcaaatg catattcaaa atcggggtgt tgtagcatgg caatattagt gccacttgct
     6181 gcaaaaatag cttctccagc ttcttgtgca ggtggcattt tgtataaagg ctcttgctcc
     6241 caggtttcac caccatcaat agttttaata ataaagaaaa accttctgcc agaattgcca
     6301 acagaaatag gatctccaat gcaaacacca gttgtattat ctaaaaaatc cattgcatct
     6361 aaaaacattc cttccatatt tctagaaaaa acagtgtacc aacttttacc accatctttt
     6421 gtttttaaaa tataagctgg gttgcctact gccattataa tagcattgtt actatcaaaa
     6481 gcatgaatat ctctaaaatc attttgctca aaatttttaa cccttgtcca ttgccatgtt
     6541 ttacctgcat ctgttgtttt gccaacagtg ccattgttgc cactaatcca aacagtattt
     6601 tcatcggcca cactcattcc tctaaaactt acttgtatcg gtgctcctgt atttaaaatt
     6661 tgaacacctt ttttttgtgc aaaaacattt gataagtaaa gagtgaaaat taaactgaat
     6721 agtattttat ttttcataag taatattatg gtgtataaaa ttaagaacaa ttaataaaca
     6781 gataattgaa ttttttttag attagtttaa aaaaaaataa tttataaaat tcatttgtca
     6841 taaaattgta tttttgtaac gttaatttac aaccacaata tttgcttatg acttggaaac
     6901 aaatgttcac atcatcggta ggcaaaaaac taaccatggg ctttaccgga atttttctta
     6961 ttttattttt aatagtacac gttggcttaa atgcatgcat ttgggctatg gacgatggca
     7021 aaatgtttaa taaagctgct gcttt
//
LOCUS       DAOQPK010000103         7005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_6096_length_7005_cov_4.863165, whole genome shotgun sequence.
ACCESSION   DAOQPK010000103 DAOQPK010000000
VERSION     DAOQPK010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6096_length_7005_cov_4.863165"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PLU36_06175"
     CDS             complement(<1..663)
                     /locus_tag="PLU36_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ46373.1"
                     /translation="MQHWTEEITPKNSLLDLKLKEVWHYRDLMFMFVKRDFVTFYKQT
                     ILGPLWFFVQPLLTTIMYVIVFGNIAKISTDGLPPILFYLCGITFWNYFSECFTKTST
                     VFKDNANMFGKVYFPRLVMPLSIVFSGLIKLSIQFLLFILLLLWYMYQSANVHPNLYI
                     LLTPYLVILMAVIALGAGMIISSLTTKYKDLIFLITFGIQLLMYATPVIYPISSLPEK
                     YAF"
     gene            complement(741..1778)
                     /locus_tag="PLU36_06180"
     CDS             complement(741..1778)
                     /locus_tag="PLU36_06180"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HMZ46374.1"
                     /translation="MNENQQIIINFLKNNPHTSSKEIMTNAGVIASYATIKRLLQSMA
                     TEGLLEIIGKGKSTKYAVSPVYELIEPISVDTYFKNEIDERKIINSFNYNIFKNLLSA
                     KNIFSNSELGILEKFHVTFKNRIAKLSKNELKKDLERLAIDLSWKSSQIEGNTYSLLE
                     TERLLKEKETAEGKTQEEAFMLLNHKEAIDFIIQHPDYFFTLTISNIEDIHKVLVQQL
                     DIDINVRNKKVGISGTNYKPLDNQFQIKEAMTSMCEIVNRKENVFEKALLTLILLSYI
                     QPFMDGNKRTSRIVSNAVLLSYGYCPLSYRTVNSIDYKKAMLLFYEQNNLSAFKNIFI
                     DQYQFAVENYF"
     gene            complement(1879..4371)
                     /locus_tag="PLU36_06185"
     CDS             complement(1879..4371)
                     /locus_tag="PLU36_06185"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HMZ46375.1"
                     /translation="MSSNLSPSPISFLSQNNNENKSKFSVRETLMKYLSYFPLFVLSM
                     VLCVGAALMYLRYKVPIYKATLQVMVQQNASSKSSSPQPDLISQAVSGVRAINLENEI
                     QLIQSKALLEKVVIAGNFNIVCSKEGNIKKSDAYKSTPFDFIPLHIPDSSKIFTVKLI
                     AYNTSGGTVITPSGNKNFKWGDSLNIYGLGFRLINNTNNFEAEQSPYIIKWVPVGFMA
                     SQIQGSLSVGTLSQKTSVLLLGLTGESRIKAIDFLNLYVKEIIKKDIELKKEASLATI
                     EFINNRLKIVEKELSDLEIQIRDYKKANRFFDASGEYNYIQIRLSKAEEEVLKLRLDI
                     QNIDYIDNYLRNDKSLGKSKVIPSNLGVEDMSYNTLIAKYNDLQAQKEKEDFVSNKDN
                     PKLIELDNQVSQFKQSILEAGAILKQSKLLKISTYDGRTDVDMEKLATLPDKELKMIE
                     INRQKAIKEKLYLYLLQKGEETAIASVSTESNYQAMDDAIASSVPVEPKSTQIKVFSI
                     ILGLLIPIGIIYLLDLLNDKITTRQDIAERTKIPITGEISHLDAVGKIVVENSRNVVA
                     EQFRILRSNLQFILPTANDGKATTILVTSSISGEGKSFISLNLAAVLALTEKKVALLE
                     FDLRKLKGINLDEIEDNNDRGITNFLIGQTNDINSICKTLKKHPNLHLYNTGPIPPNP
                     AELIINSRMEMFFNILKDKYDYIVLDSAPVGLVSDSFALSKYADVDLYVIRQRYTLKK
                     QLDFINDLHNEEKLKNMAIVVNDVNLAGRYGYYGYGYGYGYGYMYRYGMGYGYNRYIY
                     GGRKKDPYFDSNKQGYFDDNVKLKWWQKIFRK"
     gene            complement(4395..5150)
                     /locus_tag="PLU36_06190"
     CDS             complement(4395..5150)
                     /locus_tag="PLU36_06190"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HMZ46376.1"
                     /translation="MKKIVLFGFIIGLFFTSCTNYKKLEKQYQLFQTGLDSMSKYSYK
                     SLTLKPYDVIIIKTYTTATSNQDQTSLFNILGNKFTINQNGTIDLPKIGILKVEGLTC
                     KQLKTVLINEWSKYVKDILVDVQFAGLTVNVLGEVASQGTKKFEKEEVTIIDVLAASG
                     GLNLDAKRNDILVIREDSGRRQHYLLDLRDANFYTSPAYQLQQNDLVYVGASTKKFKN
                     LSGAEFRNNIMPLTQTISIVISFLNFFLLLSNK"
     gene            complement(5491..5952)
                     /locus_tag="PLU36_06195"
     CDS             complement(5491..5952)
                     /locus_tag="PLU36_06195"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HMZ46377.1"
                     /translation="MQFKAKQIGIVNVQFGENVTLVEPCNIYGCTIGNNSFVGPFTEI
                     QKDVIIGSNCKIQSHSFICELVTIGNNCFIGHGVMFINDTFSQGKPAGGIKELWKETK
                     IGNNVSIGSNATILPITICDNVVIGAGAVVTKDILKPGVYVGNPAKCIKNL"
     gene            complement(6027..>7005)
                     /locus_tag="PLU36_06200"
     CDS             complement(6027..>7005)
                     /locus_tag="PLU36_06200"
                     /inference="COORDINATES: protein motif:HMM:NF022646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HMZ46378.1"
                     /translation="HLASLPLKCIDIEFPNKTSHTSETLEDHILSPKQLHPAFYGCFD
                     WHSCVHGHWMLLKLLKSFPDLPEAGAIRKVFNKNITLQNIATEVNYFNATLSKTWERT
                     YGWAWLLKLQAELINFNDSDAQKWSKTLQPLTDSIISRWLQFLPKQTYANRTGVHANT
                     AFGLILALDYARKAKHKQLEEAIIKSAKHLFLKDKNAPALWEPNGSDFLSPSLIEADL
                     MSKILTPNNFIIWFNQFLPQKSLAHLLQLPIVSDRSDYQIVHLDGLCFSRVWCMKSIA
                     KLLPENDKRKKLLQKAAKQHLITSLPNIANGYYGGEHWLASFAVYALME"
BASE COUNT         2329 a         1112 c          934 g         2630 t
ORIGIN      
        1 aaatgcatat ttttctggta aagagctgat aggataaata acaggtgttg catacattaa
       61 aagttgtatt ccaaaagtaa ttaagaaaat aaggtcttta tattttgtag ttaatgaact
      121 aataatcatt cctgcaccta aagcaataac tgccattaaa attactaaat atggagtaag
      181 taaaatgtac aaattaggat gtacgttggc actttgatac atataccata ataaaagtaa
      241 aataaacaga agaaattgta tggatagttt tattagccca gagaatacta ttgataaagg
      301 catcaccaaa cgtggaaaat atactttacc aaacatatta gcattgtctt taaaaacagt
      361 ggatgttttg gtaaaacatt cagagaaata gttccaaaat gtaatgccac acaaataaaa
      421 taaaattgga ggaagcccat ctgtagatat tttagcaata ttgccaaaaa caataacata
      481 cataatggtt gtaagcaagg gttgtacaaa aaaccaaaga ggacctaaaa tagtttgttt
      541 ataaaaagtt acaaaatctc gtttaacaaa catgaacatt aaatctctgt aatgccatac
      601 ttcttttagt tttaaatcta gcaaggagtt tttgggtgtt atttcttctg tccagtgttg
      661 catgtattct attttatttg tcttgattta ttggtattaa ttaaattatc ttttttaaaa
      721 aaaattttac ccttttagtt ttagaaataa ttttctactg caaattgata ttgatcaata
      781 aaaatatttt taaaggcaga taaattattt tgctcgtaaa ataataacat agcttttttg
      841 taatctatag aatttacagt tctgtaggaa agtgggcaat aaccataact taataatact
      901 gcattgctaa caattcttga agttctttta ttgccatcca taaagggttg tatgtaggat
      961 aaaagaatta aagtaagtaa tgctttttca aaaacatttt ctttccgatt tacaatttca
     1021 cacatagaag tcatggcttc ttttatctga aactgattat ctaaaggttt ataattggta
     1081 ccagaaatac ccactttttt atttctaaca tttatatcaa tatctaattg ctgtactaaa
     1141 actttatgaa tatcttcaat attagagatt gttaaagtaa aaaaataatc tgggtgctgt
     1201 ataataaaat caatagcttc tttatgattt aaaagcataa aagcctcttc ttgtgttttg
     1261 ccctctgctg tttctttttc ttttaatagc ctttctgttt ctaataaaga ataggtattg
     1321 ccttctattt gagatgattt ccaagataaa tctatagcta aacgttccaa atcttttttt
     1381 aattcatttt tagaaagctt tgctattcta tttttaaaag taacatgaaa tttttctaag
     1441 atacctaatt ctgaattgct aaaaatattt ttagctgata ataagttttt aaaaatgtta
     1501 taattgaaag agttaattat ttttcgttca tcaatttcat ttttaaaata agtatccacg
     1561 gatataggtt caattaattc atatacaggc gatactgcat actttgtgct tttccctttt
     1621 ccaataattt ctaataaccc ttcagttgcc attgattgaa gtaaacgttt tatagttgca
     1681 taacttgcta taactccagc attagtcata atttcttttg aagaagtatg agggttattc
     1741 ttgagaaaat taataattat ctgctgattt tcgttcacaa cttaaattta tatagctcaa
     1801 ttaatagtta cttaaagttc attttaaggt aaaattgagc catattttag gaaaacttga
     1861 gctcaaaata tttaagaatt attttctaaa tattttttgc caccatttta gttttacatt
     1921 atcatcaaaa tagccttgct tattactatc aaaataagga tctttttttc ttcctccata
     1981 aatgtatcta ttgtagccat aacccattcc atatctatac atataaccat aaccataacc
     2041 atagccataa ccataatagc catatcggcc tgcaagattt acatcgttta ccacaatggc
     2101 catatttttt aatttctctt cattatgtaa gtcattaata aagtctaatt gtttttttaa
     2161 tgtatatcgc tggcgaataa cgtacaaatc aacatcggca tatttactta atgcaaaaga
     2221 atcgcttact aagcctactg gtgcagagtc taatacaata taatcgtatt tatcttttaa
     2281 aatattaaaa aacatttcca ttctactgtt aataatcaac tcagcaggat taggaggaat
     2341 aggacctgta ttatataaat gtaaattagg atgttttttt aaagttttac aaatagaatt
     2401 aatgtcatta gtttgcccta taagaaaatt ggtaatacct ctatcattat tatcctctat
     2461 ttcatctaaa ttaatacctt taagtttacg taagtcaaac tctaaaagag ctactttttt
     2521 ttcggttaaa gccaatactg ctgcaagatt taagctaata aatgattttc cctctccact
     2581 aatggatgag gtaactaata ttgttgttgc tttgccatca ttggctgtag gcaaaataaa
     2641 ttgcaagttg cttcttaata ttctaaactg ttcggctact acatttctac tgttttctac
     2701 tacaatttta cccactgcat ctaagtggct tatctcacct gtaatgggta ttttagttct
     2761 ttcagcaata tcttgccttg tagtaatctt atcgttcagt aagtctaata aataaataat
     2821 accaatagga atgagtaatc ctaaaataat ggaaaatact ttaatttgag tacttttagg
     2881 ctctacagga actgatgaag caattgcatc atccattgct tgatagttag actctgtaga
     2941 tacggaagca atggctgttt cctctccttt ctgtaagaga tataaatata atttttcttt
     3001 aattgctttt tgcctgttta tttcaatcat ttttaattct ttgtcaggca gtgtagctaa
     3061 cttttccata tctacatctg ttcttccatc ataggtactt atttttaata attttgattg
     3121 ttttagaata gctcctgctt ctaaaatact ttgtttaaac tgactaactt gattatctaa
     3181 ctctataagt ttagggttat ctttattgga tacaaaatct tctttttctt tttgtgcttg
     3241 taaatcgtta tacttggcta ttaatgtatt gtacgacata tcctcaactc ctaagttaga
     3301 aggaatgact tttgatttac ctaagctttt atcgtttctt aaatagttgt cgatataatc
     3361 tatattttga atatctagtc taagtttcaa aacttcttct tccgctttag acaatcttat
     3421 ttgtatataa ttatattcgc cagatgcatc aaaaaatctg tttgcttttt tatagtctct
     3481 tatttgtatt tcaaggtcgc ttaattcttt ttctactatt tttaatctat tattaataaa
     3541 ttctatagtt gctaaggagg cttctttttt aagttcaata tcttttttaa taatttcttt
     3601 tacatacaga tttaaaaaat caattgcctt aattcggctt tcgcctgtta atcctaagag
     3661 taaaacactt gttttttggc ttaaagtacc aacagataaa ctaccttgaa tttgagatgc
     3721 cataaagcca actggcaccc atttaataat gtaaggagat tgttccgctt caaaattatt
     3781 tgtattgtta attagcctaa atcctaagcc ataaatattt aaggaatctc cccatttaaa
     3841 gtttttattt ccagatggag ttatcacagt tcctccgctt gtattatatg caattagttt
     3901 tacagtgaaa attttagatg agtctggaat atgtaaagga ataaagtcaa atggagtaga
     3961 tttataagca tctgattttt ttatattacc ctctttgcta cacacaatat taaaattacc
     4021 agcaataacc actttttcta ataaagcttt tgattgtatt agctgtattt cattttcaag
     4081 attaatagct cttacacctg atacagcctg gcttattaaa tcgggttgag gagaagatga
     4141 tttgctactt gcattttgct gtaccattac ttgcaaagtg gctttgtata ttggtacttt
     4201 gtaccttaag tacattaatg ctgcacctac acataaaacc atagaaagta caaataatgg
     4261 gaagtaactt aagtacttca ttaaagtctc tctaactgaa aactttgatt tgttttcgtt
     4321 attattttga gataaaaaag aaataggaga tggtgaaaga ttagaactca ttaactctta
     4381 tattgaatga ttttttactt gtttgaaagt aataagaaaa agtttaaaaa gcttattact
     4441 atactaatag tttgtgttaa aggcataata ttatttctaa actctgcccc acttaaattt
     4501 ttaaactttt tggtactagc acccacataa accaaatcgt tctgttgtaa ttggtatgct
     4561 ggtgaagtat agaaatttgc atctcttaag tctaataaat agtgctgcct tctcccacta
     4621 tcttctctta ttactaaaat atcatttctt ttggcatcta aattcaaacc tccagaagca
     4681 gccaaaacat caataatggt tacttcttct ttttcaaatt tttttgttcc ctgagaggct
     4741 acctcaccta acacatttac agttaaacca gcaaattgca catctaccaa aatatctttt
     4801 acatacttgc tccactcgtt aattaaaact gtttttagtt gtttacaagt caagccttct
     4861 accttaagta tcccaatttt aggtaaatca attgtaccat tttgattgat tgtaaattta
     4921 tttcctagta tattaaataa gcttgtttga tcttgattag aagttgctgt tgtataagtc
     4981 ttaataataa taacatcata aggctttaag gttaaacttt tgtaggagta tttactcata
     5041 ctatctaacc ctgtttgaaa taactggtat tgtttttcta gctttttata attagtacag
     5101 gaagtaaaaa acaggccgat aataaaacca aataaaacaa tcttcttcat atccactaat
     5161 agtttaaaaa ataagagtta atttaaaaaa tgtacagctc cgccacctga ctcacattgc
     5221 tgtcagtaaa taccctaaaa aacataggca taacaggtaa ttaaaaatta aattttaccc
     5281 gtatgcctat tataaattta tatcaaatac ttaattttta gttctttcat aaacactaaa
     5341 actaaactag gtttagctac actgtttcat tactcaattt tagggagtcg caaatatagg
     5401 tatttaagtc agaaaatatt ttttttcatt tgcttttttg tcctaatata ttcccattat
     5461 tattttaaaa aaaatacgca actaattctg ttacaaattt ttaatacatt tagcagggtt
     5521 accaacatat acaccaggct tcaaaatatc tttagtaact acagcaccag cacctattac
     5581 tacattatcg catattgtta tgggcaaaat tgttgcatta ctacctatag atacattatt
     5641 tccaattttc gtttctttcc ataattcttt tattcctcct gcaggtttac cttgactaaa
     5701 agtatcgtta ataaacataa cgccatggcc tataaaacaa ttattaccaa tagtaaccaa
     5761 ttcgcaaata aaactatgcg attgaatttt acaattacta ccaataataa catctttttg
     5821 tatttcagtg aaaggtccta caaaactgtt attacctata gtacaaccat aaatattaca
     5881 aggctctact aaagtaacat tttctccaaa ttgtacgttt acaatgccta tttgcttggc
     5941 tttgaactgc ataacaaatt aatttttggt ttgcaattta caacctccaa tattattatt
     6001 tgaattaagt tttgaatact gaaaaactat tccattagtg catacactgc aaagcttgca
     6061 agccaatgtt cgcctccata ataaccattg gcaatattgg gcaagctagt tattaaatgt
     6121 tgcttggctg ctttttgaag aagctttttt cttttatcat tttcgggtaa aagttttgca
     6181 atgcttttca tacaccatac tctgctaaaa cacaagccat ctagatgtac aatttgataa
     6241 tcacttctat cacttacaat gggtaattgc agcaaatgtg ctaaagattt ttgtggtaag
     6301 aattggttaa accagattat aaaattatta ggggttaaaa ttttgctcat taaatctgcc
     6361 tcaattaatg aaggggataa aaaatcgctt ccatttggct cccacaatgc cggtgcattt
     6421 ttatctttta aaaataaatg ctttgccgat ttaataattg cttcttccaa ttgcttatgc
     6481 tttgcctttc tagcataatc aagtgctaaa attaggccaa atgcagtatt ggcatgtaca
     6541 ccagtgcgat ttgcataagt ttgtttgggt aaaaattgaa gccatctgct aattatagaa
     6601 tctgttaatg gttgtaaagt tttactccat ttttgagcat cagaatcatt aaaatttatt
     6661 agctctgctt gcagttttaa taaccatgcc caaccataag ttctttccca tgttttagat
     6721 aatgttgcat taaaataatt tacttcggtt gctatatttt gtaaagtaat attcttgttg
     6781 aaaacctttc ttattgcacc agcttctggt aaatctggaa aactttttaa taatttcaat
     6841 aacatccaat gcccatgtac acagctatgc caatcaaagc atccataaaa tgcagggtgc
     6901 aactgtttgg gagaaagaat atgatcttct aaagtttctg acgtatggct tgttttatta
     6961 ggaaactcaa tgtctataca ttttagagga agagatgcta gatgg
//
LOCUS       DAOQPK010000104         6955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_6157_length_6955_cov_4.812464, whole genome shotgun sequence.
ACCESSION   DAOQPK010000104 DAOQPK010000000
VERSION     DAOQPK010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6955
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6157_length_6955_cov_4.812464"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="PLU36_06205"
     CDS             <1..696
                     /locus_tag="PLU36_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HMZ46379.1"
                     /translation="KRKAEQGSMQLQGEVQEILLEELLTVNFPFDTIVEVEKGIKGAD
                     CVQIVKTQFGFEAGKIIFESKRTKDFNIEWIEKLKKDMRQLGADIAVIVTQTFPKDMD
                     RFGEKDGVYICSISEVKSMITLLRAALIKIYDAKKSQENKGDKMVLLYDYLTGNEFNQ
                     QWKAIREGFMAIKSSIAKERDAMEKLWKVREKQLEKVLLNAAHIHGSVEGIAGIEAMN
                     LTLDFEDKNLLDE"
     gene            complement(703..1665)
                     /locus_tag="PLU36_06210"
     CDS             complement(703..1665)
                     /locus_tag="PLU36_06210"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ46380.1"
                     /translation="MQNFNFTYNNFEELLKQYAKSFNVRIKDKAVILPEQSGKGSICY
                     YQLNNGFQVLVNNFSVFNDITFQHQKTNEEFFILRLDEIVTQEEIDDESKSAAVLANT
                     SFDWIFYVKKGAQINSIHVLFTKEWLNNFLDGEKKVDYIKKYLSLKLATFNYESLDAE
                     YKRLMKEVLLCNDKTPLKLLVLQNRIMLLVERFFTRMILKINDNSFEVKVSDDDLKRL
                     VLVEEELLKDFSIPPPGIPKLARMAAMSPSKLKIIFRDIYGLPIYQYFQKHRMNRAKA
                     MLISQKYTVKEVGFELGYTNLSNFAKAFKKSFDQLPSDLFEVKT"
     gene            complement(1722..1943)
                     /locus_tag="PLU36_06215"
     CDS             complement(1722..1943)
                     /locus_tag="PLU36_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC family transcriptional regulator"
                     /protein_id="HMZ46381.1"
                     /translation="MNKLRAFLETSAFGVCSNIGDKLGIASSKIRMWFIYISFATMGS
                     PIIVYMILAFWLNIKKYIYFAKRNPLRYL"
     gene            2098..4365
                     /locus_tag="PLU36_06220"
     CDS             2098..4365
                     /locus_tag="PLU36_06220"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HMZ46382.1"
                     /translation="MAFDIEMIKKAYAEMPEKINAARTKMGRSLTLSEKILFAHLHSD
                     MKLENFERGKSYVDFAPDRVAMQDATAQMALLQFMQAGRSKVAVPSTVHCDHLIMAKL
                     NAKNDLEIANQESKEVYDFLASVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTD
                     SHTVNAGGLGMIAIGVGGADACDVMAGLAWELKWPKLIGIKLTGKLSGWAAPKDVILK
                     VAGLLTVKGGTGAIVEYFGEGATAMSCTGKGTICNMGAEIGATTSTFGYDESMSRYLK
                     STGRADVAALADNIKEHLTADAEVYTNPEKYFDQVIEINLSELEPHLNGPFTPDLATP
                     ISKMKEEAAKNGWPTKVEVGLIGSCTNSSYEDISRAVSLAKQVSEKGLKTKADYTITP
                     GSEQVRYTIQRDGFLEVFDKIGATVFANACGPCIGMWDRVGAEKKEKNTIVHSFNRNF
                     AKRADGNPNTFAFVASPEIVTALAIAGDLTFNPITDTLVNEKGELVKLDEPTGDELPS
                     KGFAVEDAGYQAPAADGSNVVVNVSPTSNRLQLLDPFAAWDGNDLKNLKLLIKAKGKC
                     TTDHISMAGPWLKFRGHLDNISNNLLIGAINFFNDKADNVKNQLTGEYGTVPATQRAY
                     KAAGIGSIVVGDENYGEGSSREHAAMEPRHLGVKVVLTKSFARIHETNLKKQGMLALT
                     FANKEDYDKILEDDTIDVIGLTTFSPNDALTLVLHHKNGIKEEIVANHTYNQQQIEWF
                     KAGAALNIIRKQVAY"
     gene            complement(4443..4724)
                     /locus_tag="PLU36_06225"
     CDS             complement(4443..4724)
                     /locus_tag="PLU36_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46383.1"
                     /translation="MDFIVETLKNLIPYFLLSIVFVFVLKSFLVTKVKSFDLAEIFFS
                     FFRLYNYDEINMSSNKRRVTFMRWNNLFNYYTYFVIGLVILVYLVTKDA"
     gene            4834..6618
                     /gene="lepA"
                     /locus_tag="PLU36_06230"
     CDS             4834..6618
                     /gene="lepA"
                     /locus_tag="PLU36_06230"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HMZ46384.1"
                     /translation="MKHIRNFCIIAHIDHGKSTLADRLLEHTKTINERKMQAQVLDDM
                     DLEREKGITIKSHAIQINYTYNNQQYVLNLIDTPGHVDFSYEVSRALAACEGALLLVD
                     ASQGIQAQTISNLYLALDNDLEIIPVINKIDMEGAMVLEVTDQIVDLIGCKPEEILLA
                     SGKSGIGIEEILQAIVHRIPSPKGNTEAPLQALIFDSVFNSFRGIIIYYRILNGCIKK
                     GDKIRFCATGEDYEAMEVGILKFGMEEKKEISCGDVGYLITGIKNAKEVKVGDTITLA
                     NNPADAIKGFEEVKPMVFAGIFPVITDDFEELRDCMEKLQLNDASLTFELETSQALGF
                     GFRCGFLGLLHMEIIQERLEREYNQTVITTVPNVSFFAYTTRGEKIVVNNPAEMPETT
                     KIEKIEEPFIKAQIITHSDYIGNIMTLCLGKRGILINQSYITTTRVELIFEMPLTEIV
                     FDFYDKLKSQTRGYASFDYSPIGYRDADIVRMDILLNAEKVDALSALIHRSRSQDFGR
                     KLCEKLKELLPRQQFQIAIQAAIGAKIIARENISAIRKDVTAKCYGGDISRKRKLLEK
                     QKEGKKRMRQIGTVEVPQEAFLAVLKLD"
     gene            complement(6624..>6955)
                     /locus_tag="PLU36_06235"
     CDS             complement(6624..>6955)
                     /locus_tag="PLU36_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46385.1"
                     /translation="LANLNYNKPYFSFGNNLFSNSTIKNAIQFTGNIYQYIDSTNFYQ
                     FNGEQGIAFYNWPNDSLLKNNLINKANSFALLKSDSCLKKKIQFYNSAMINNKMNISS
                     IRINQKN"
BASE COUNT         2617 a          925 c         1201 g         2212 t
ORIGIN      
        1 aaaagaaaag cagagcaagg aagcatgcag ttgcaaggag aagtgcagga gattttatta
       61 gaagaattat taactgttaa ttttcctttt gatacaattg tagaagtaga aaaaggaatt
      121 aaaggagcag attgtgtaca aattgtaaaa acacaatttg gctttgaagc tggaaaaata
      181 atttttgaga gtaaaagaac caaagatttt aatatagagt ggatagaaaa gttaaaaaaa
      241 gatatgaggc aattaggtgc cgatattgca gtaattgtta cacaaacttt tccgaaagat
      301 atggataggt ttggcgaaaa agatggcgtt tatatatgtt ctatttctga agttaagagc
      361 atgataactt tattaagggc tgcgttaatt aaaatatatg atgctaaaaa aagtcaggaa
      421 aataaaggcg ataaaatggt tttattatac gattatttaa caggcaatga atttaaccaa
      481 caatggaagg ctataagaga aggctttatg gcaattaaat cctctattgc aaaagaaaga
      541 gatgcaatgg aaaaactttg gaaagtgaga gaaaaacaat tagaaaaagt attacttaat
      601 gcagctcata ttcatggctc agtagaggga atagcaggta ttgaagctat gaatttaacc
      661 ttagattttg aggataaaaa tttactagat gaataataag gattaagttt ttacttcaaa
      721 caaatcgcta ggtaattggt cgaaagattt tttaaaagct ttagcaaaat tgcttaagtt
      781 ggtataaccc aattcaaaac ctacttcttt tacagtatat ttttgagata ttaacatagc
      841 cttggctctg ttcattctgt gcttttgaaa atattgataa ataggtaatc cgtaaatatc
      901 tctaaaaata attttaagtt tagaagggct catagcagcc attcttgcaa gtttaggaat
      961 acctggtgga ggaattgaaa aatctttcag cagttcttct tctaccaata ccaacctctt
     1021 caaatcatca tcagatactt ttacctcaaa actattatca tttattttaa gaatcattct
     1081 ggtaaaaaat ctttctacta aaagcattat cctattttgt aaaaccagta attttaaagg
     1141 tgttttatca ttgcaaagta aaacctcttt cattaacctc ttatattcag catctaaaga
     1201 ttcgtaattg aaagttgcca atttaaggct caaatatttc ttaatataat caaccttttt
     1261 ttcgccatcc aaaaaattat tcaaccattc tttagtaaat aatacatgaa tactattgat
     1321 ttgtgcccct ttttttacat aaaatatcca gtcgaaagaa gtattagcta atacagcagc
     1381 acttttagat tcatcgtcta tttcttcttg agtaacaatt tcatcaagtc ttaaaataaa
     1441 aaactcttcg ttagtttttt gatgctgaaa agtaatatcg ttgaatacag aaaaattatt
     1501 aactaaaacc tgaaagccat tatttaattg ataataacaa atggaacctt ttccagattg
     1561 ttctggcaaa ataacagctt tgtcttttat tctaacatta aaagatttag catattgctt
     1621 taaaagctct tcaaaattat tgtatgtaaa attaaaattc tgcaatttga gatgaagtaa
     1681 tttggatttt aaaattaaaa aaattaaaac tttttaaaac attataaata ccttaatggg
     1741 tttcgtttag caaaataaat atacttttta atatttaacc aaaaggctaa aatcatatac
     1801 actataattg gagagcccat tgtagcaaat gaaatgtaaa taaaccacat tctaatttta
     1861 gaacttgcaa tacctaattt atcaccaatg ttactgcata caccaaatgc acttgtttct
     1921 aaaaatgctc taagtttatt catgtttttt aatctaaaaa tctatttaat atgcaaaata
     1981 taaaattata actaaattct ttaaaacata tttatattaa aggtcattta tttgtaattt
     2041 tgctgcctgt aaaaataatt tttagtattt tttaaaacca atcaaaattt tttatttatg
     2101 gcttttgata ttgaaatgat taaaaaggct tatgcagaaa tgccggaaaa aataaatgct
     2161 gctagaacta agatggggcg ttctcttaca ttatccgaaa aaatattgtt tgctcactta
     2221 catagcgaca tgaaattgga aaattttgaa agaggaaaaa gctatgtaga ttttgcacca
     2281 gaccgtgtag cgatgcaaga tgcaactgca caaatggcat tattacagtt tatgcaagct
     2341 ggtcgttcaa aagttgcagt gccaagtaca gtacattgcg accatctaat aatggctaaa
     2401 ttaaatgcaa aaaacgattt agaaattgct aatcaagaaa gcaaagaagt gtatgatttt
     2461 cttgcttcag tatctaataa atatggaatt ggattttgga aaccaggtgc aggaattata
     2521 catcaggttg ttttagaaaa ttatgcattt cctggaggta tgatgatagg aacagatagc
     2581 catactgtta atgcaggtgg cttaggcatg attgcaatag gcgttggtgg tgcagatgct
     2641 tgtgatgtaa tggctggctt agcttgggag cttaaatggc caaagctaat tggtataaaa
     2701 ctaacaggta aacttagtgg ttgggcagct cctaaagatg ttattttaaa agtagcaggt
     2761 ttgttaactg taaaaggtgg cacaggagct attgtggaat attttggtga aggtgctaca
     2821 gcaatgagtt gtacaggtaa aggaactatt tgtaatatgg gagcagaaat tggtgcaacc
     2881 acttctacat ttggttatga tgaaagcatg agcaggtatt taaaatctac aggaagagct
     2941 gatgtagctg cattagcaga taatattaaa gaacatttaa ctgccgatgc agaagtttat
     3001 actaatcctg aaaaatattt tgaccaagtt attgaaataa atttaagcga acttgagcct
     3061 catttaaatg ggccattcac gccagattta gcaactccaa tttctaaaat gaaagaagaa
     3121 gctgcaaaaa atggttggcc aactaaagta gaagttggct taattggtag ctgtactaat
     3181 agttcttacg aagatatttc aagagctgta agtttagcca aacaagtatc tgaaaaaggg
     3241 ttaaagacaa aggcagatta tactattaca ccaggcagtg agcaagttag atatactatt
     3301 caaagagatg gttttttaga agtatttgat aaaattggag ccaccgtttt tgcaaatgct
     3361 tgtgggcctt gtattggtat gtgggataga gtaggagcag aaaagaaaga aaaaaataca
     3421 attgttcata gctttaatag aaattttgcc aaacgagccg atggtaatcc aaatactttt
     3481 gcttttgtag caagccccga aattgtaact gcattagcta ttgcaggaga tttaactttt
     3541 aatccaataa ccgatacttt agttaatgaa aaaggagaac ttgtaaaatt agatgaacct
     3601 acaggcgatg aattaccatc taaaggcttt gcagttgagg acgcaggcta tcaagcacct
     3661 gctgcagatg gtagtaatgt tgttgtaaat gtttcgccaa ctagcaatcg tttacaacta
     3721 ttagatcctt ttgctgcttg ggatggtaat gatttaaaga atttaaaact tttaataaaa
     3781 gcaaaaggga aatgtactac cgaccatatt tctatggctg gcccatggtt aaaatttaga
     3841 ggccatttag ataatatctc taacaattta ttaataggtg caattaactt ttttaatgat
     3901 aaagcagaca atgtaaaaaa tcaattaaca ggagaatatg gtactgtacc tgctacacaa
     3961 cgtgcctaca aagctgcagg cattggaagc attgtagttg gagatgaaaa ctatggagaa
     4021 ggctcttcta gagaacatgc tgcaatggag cctcgtcatt taggagtaaa agttgttcta
     4081 acaaaatctt ttgctcgtat tcatgaaact aatcttaaaa agcagggtat gttggcttta
     4141 acttttgcta ataaagaaga ttatgataaa atattagaag atgatacaat tgatgtaata
     4201 ggcttaacca ctttttcgcc caatgatgct ttaacattgg ttttacacca taaaaatggc
     4261 ataaaagaag aaattgtagc aaaccatact tataaccaac aacaaataga gtggtttaaa
     4321 gctggtgctg ctttaaatat tattcgtaag caagttgctt attaaataaa tattttaggc
     4381 aaacaaaaat cccatgttac atagtaacat gggatttttt tatacaacga tttatacaaa
     4441 ttttaagcat ctttagtaac caaataaact aaaataacca atccaataac aaaataagta
     4501 tagtaattaa aaaggttatt ccaacgcata aatgttactc ttcttttatt gctgctcata
     4561 ttaatttcat cgtaattata caagcgaaaa aagctgaaaa atatttcagc taaatcaaaa
     4621 cttttaacct tagttaccaa aaagcttttt aaaacaaaaa caaaaacaat actcaataaa
     4681 aaataaggta tcaaattttt taatgtttct acaataaaat ccattagttt tttaaataat
     4741 ttattataat aataagggaa taaataaaaa ttgagcaaat aataaaaaca aatataagta
     4801 aaaaaatttt gtattgttat ctttgccgca attatgaagc atatacggaa tttttgcatt
     4861 attgcacaca tagatcatgg taaaagcact ttagcagata gattgttaga acacactaaa
     4921 accattaacg aaagaaaaat gcaggctcag gttttagatg atatggactt agaaagagaa
     4981 aaaggaatta ctattaaaag ccatgcaatt caaattaatt atacttacaa taatcaacaa
     5041 tatgtattaa acttaattga tactccagga catgttgatt ttagttacga agtaagccgt
     5101 gcattagcag catgcgaagg tgctttgtta ttggtagatg caagccaagg cattcaagca
     5161 caaaccatca gcaatcttta ccttgcatta gataatgatt tagaaattat tcctgtaatc
     5221 aataaaatag atatggaagg tgcaatggtt ttagaagtaa ccgaccaaat tgttgatttg
     5281 ataggatgta aaccagaaga aattttattg gcaagtggta aaagcggaat tggtattgaa
     5341 gaaattttac aagccattgt gcatagaatt ccatcgccaa aaggtaacac agaagcaccc
     5401 ttacaagcat taatttttga tagtgttttt aacagcttta gaggtataat tatttattat
     5461 agaatattaa atggttgtat taaaaaagga gataaaatta gattttgtgc aacaggagaa
     5521 gactatgagg caatggaagt aggaatttta aaattcggga tggaagaaaa aaaggaaatt
     5581 agttgtggtg atgttggcta tttaataaca ggtataaaaa atgctaaaga agtaaaagtt
     5641 ggcgatacta ttactttggc aaataaccct gcagatgcaa ttaaaggctt tgaagaagtt
     5701 aaaccaatgg tttttgctgg aatttttcca gtaattaccg atgattttga agagttgaga
     5761 gattgtatgg agaagctgca attaaatgat gcttccttaa cctttgaatt agaaacatct
     5821 caggcacttg gttttggatt tagatgtggg tttttaggtt tattacacat ggaaattatt
     5881 caagagcgtt tagaaagaga atataaccaa actgtaataa ctactgttcc taacgttagc
     5941 ttttttgcat atacaacaag aggcgaaaag atagtggtga acaacccagc agaaatgcct
     6001 gaaacaacaa aaattgaaaa aatagaagaa ccttttatta aggcacaaat aattacccat
     6061 tcagattata ttggtaatat tatgacactt tgcttaggta aacgtggcat actaattaat
     6121 caatcttaca ttactacaac aagggttgaa ctaatatttg aaatgccctt aacggaaata
     6181 gtatttgatt tttatgataa gttaaaaagc caaacaaggg gttatgcctc atttgattat
     6241 tctccaattg gctatagaga tgcagatatt gttagaatgg atattttatt aaatgcagaa
     6301 aaagtagatg ctttaagtgc tttaattcat cgtagtcgtt ctcaagattt tggaagaaaa
     6361 ttatgtgaaa aactgaaaga acttttacct cgccaacaat ttcaaattgc tatacaggcc
     6421 gcaattggtg caaaaataat tgcacgtgaa aatattagtg ccattagaaa agatgtaact
     6481 gcaaaatgtt atggtggcga tataagcaga aaaagaaaat tattagaaaa acagaaagaa
     6541 ggcaaaaaac gaatgagaca aattggaact gtagaagtac cacaagaagc ctttttagca
     6601 gtgttaaagt tagattaaac tccttagttt ttctgattga ttctaatgct agaaatattc
     6661 attttgttat taatcattgc actattataa aactgaattt tcttttttaa gcaactatca
     6721 cttttaagta aagcaaagga attagcctta ttaattaaat tgttttttag taaactatca
     6781 tttggccaat tataaaaagc aatgccttgt tctccattaa actggtaaaa attagttgaa
     6841 tctatatact gataaatgtt acctgtaaat tgaattgcat tttttatggt agaattacta
     6901 aataaattat ttccaaagga aaaatagggt ttattatagt ttaaattagc taaaa
//
LOCUS       DAOQPK010000105         6862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_6295_length_6862_cov_4.063317, whole genome shotgun sequence.
ACCESSION   DAOQPK010000105 DAOQPK010000000
VERSION     DAOQPK010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6862
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6295_length_6862_cov_4.063317"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..2481)
                     /gene="lon"
                     /locus_tag="PLU36_06240"
     CDS             complement(82..2481)
                     /gene="lon"
                     /locus_tag="PLU36_06240"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HMZ46386.1"
                     /translation="MIKTNLLNSDEEVDFMPIIPIGENDSEQDANLKIPEELIILPLR
                     NTVLFPGVVLPITVGRDKSIKAVTDAYKADKLIGVLAQKDSSIEEPEAKDLCNIGTVA
                     KIVKLIKMPDGGTTIIIQGKKRFELISITKVDPYFKAKINILKEDEKPNTENFDAMVD
                     SIKDLATQIIQLSPNLPTEASIILKNIESPSFLINFVSSNQNTELAEKQALLEMNDIT
                     IRAEKLIQLLQRELQFAELKNKVTNKTRTELDKQQREYFLQQQLKSIKDELGGDHNEK
                     EVAEMKKKAETKKWANAAKELFNSNIAKLERMHPSTPDYSVIYNHCNLMLDLPWEDYT
                     ADNYDLKNAKKVLDKEHYGMYKIKNRILEYLAVLKLKGDMKSPILCFVGPPGIGKTSL
                     GKSIANAVGRKYTRVSLGGLHDESEIRGHRKTYIGAMPGRILQNLRKVKSGNPVMILD
                     EIDKVGNDHKGDPSSALLEVLDPEQNNSFYDNYLELEYDLSKVLFIATANSIGSIQPA
                     LRDRLEIIDLSGYAIEEKIEIAKAHLIPKQKEAHGLGKINLKISDAVLQKIIENHTRE
                     SGVRELDRQLASIMRYGAKELATKNKLKPSVTNADIEKILGTPRYNNDIYKAINLPGV
                     AVGLAWTYVGGDILFIEVALSEGKGELKLTGNLGNVMKESATTALTYLQVNAKKLGID
                     ADNFSKKNIHIHVPEGATPKDGPSAGITILTALTSAFTNKKVKPFLAMTGEITLRGQV
                     LPVGGIKEKVLAAKRAGLKEIIMCWQNEKDVLEIDSNFIKGIKFNYVKNMQQVLDIAI
                     I"
     gene            2714..2950
                     /gene="rpmB"
                     /locus_tag="PLU36_06245"
     CDS             2714..2950
                     /gene="rpmB"
                     /locus_tag="PLU36_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018677334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HMZ46387.1"
                     /translation="MARVCQITGKKPLTGNSVSHSNIKTKRRFLPNLQTKRFFFTEEN
                     RWITLKLTTEGIRTINKNGLATVIKDLRAQGEKI"
     gene            2988..3170
                     /gene="rpmG"
                     /locus_tag="PLU36_06250"
     CDS             2988..3170
                     /gene="rpmG"
                     /locus_tag="PLU36_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HMZ46388.1"
                     /translation="MAKKGNRVQVILECTEHKTSGKPGTSRYITVKNKKNTPERLELK
                     KFNPILKKVTLHKEIK"
     gene            3173..3346
                     /locus_tag="PLU36_06255"
     CDS             3173..3346
                     /locus_tag="PLU36_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 family protein"
                     /protein_id="HMZ46389.1"
                     /translation="MAKAASKNSKVKDLKASAEAKNWTKVIKSVRSPKTGAYTFKEAI
                     VHKDKVKDYLASK"
     gene            3498..4460
                     /gene="ftsY"
                     /locus_tag="PLU36_06260"
     CDS             3498..4460
                     /gene="ftsY"
                     /locus_tag="PLU36_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HMZ46390.1"
                     /translation="MGFFNKLFGKKEKETLNDGLQKTKEGFFTKLTKAIAGKSTVDAE
                     VLDNLEEALIGADVGVETTLQIIEGIEKRVKEEKYISTAELNKVLQNEIQKMLTDAND
                     DAYRNFEIPTNKKPYIILVVGVNGVGKTTTIGKLAHNYKKAGYNVLLGAADTFRAAAV
                     DQLTIWSERVGVPIVKQEMGSDPSAVAFDTVQSAIAKGSDVVLIDTAGRLHNKTHLME
                     ELSKIKRVIKKNISDAPHEVMLVLDGSTGQNALEQAKQFTAATDVNALTITKLDGTAK
                     GGVVLAIANQFKIPVKFIGVGEKIDDLIIFDKQEFVDSLFKLEN"
     gene            4495..5805
                     /gene="rimO"
                     /locus_tag="PLU36_06265"
     CDS             4495..5805
                     /gene="rimO"
                     /locus_tag="PLU36_06265"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HMZ46391.1"
                     /translation="MKAKSLKQDKVNIITLGCSKNLVDSEVLSGQLLANEIDVVHENK
                     KLDHNIVVINTCGFIDKAKEESINTILDQLELKRRGKLDKVYVTGCLSERYREDLAKE
                     IAEVDAWFGTLELPLILKQFNADYKTELLGERLLSTPKHYAFMKISEGCNRTCSFCAI
                     PLMRGNHISKPIEVLVKEAETLVQKGVKEVMLIAQELTYYGLDIYKKRSLPKLLNALA
                     DVKGLEWIRLHYAYPSKFPLEIVEVMKERENICNYLDMPLQHASDAMLKAMRRNITRE
                     EMNELIYKIRNIIPDICLRTTLISGFPGETEKDVEELKDFLKIHRFDRVGIFTYSHEE
                     NTGAYTLEDTIPQTEKEARAQEIMEVQQEISLEKNQAKVGKTFKVIIDKKEAGRYLGR
                     TEFDSVEVDNEVVIQSDKKLTIGSFVNVKITKAYDYDIEGVVVE"
     gene            complement(5825..6334)
                     /locus_tag="PLU36_06270"
     CDS             complement(5825..6334)
                     /locus_tag="PLU36_06270"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HMZ46392.1"
                     /translation="MSLNFETNLTNIQQLASNKEKENDDFISYLKSFDGEYIDKQVHL
                     LNEIVSPKIDCTLCGNCCKTLLINVQEIEANNLAAHLEINRAIFDEQFIEKGSNGMML
                     IKTVPCSFLKDNKCTVYNFRFEGCKEFPAMHLPNFKDRLFTTFMHYNRCPIIYNIIEQ
                     LKLVLNFKE"
     gene            6489..>6862
                     /locus_tag="PLU36_06275"
     CDS             6489..>6862
                     /locus_tag="PLU36_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ46393.1"
                     /translation="MSEIKKMTNYTEDDIKGLDWKEHIRLRPGMYIGKLGDGSSADDG
                     IYVLLKEVLDNCVDEHTMGFGKHIDISIENKTVTIRDFGRGIPLGKVVDVVSKINTGA
                     KYDSKAFQKSVGLNGVGTKAVNA"
BASE COUNT         2418 a          987 c         1095 g         2362 t
ORIGIN      
        1 aaataattaa ttggaactat ttaaataaaa aatgcttctc tattattttt agaagcattt
       61 tttataaata aattgtttat tctaaattat agctatatct aaaacctgtt gcatattttt
      121 tacataatta aatttaatac cctttataaa gttgctgtca atttctaaaa catctttttc
      181 attttgccag cacataatta tttcttttaa tccggcacgt tttgcagcta atactttttc
      241 tttaattcca ccaacaggca gcacttggcc acgtaaggtt atttcgccag tcatggctaa
      301 aaagggtttt acttttttat ttgtaaaggc agatgttaat gcagtaagaa tggtaattcc
      361 tgcactaggc ccatcttttg gggttgctcc ttctggtaca tgaatatgaa tatttttttt
      421 gctaaaatta tctgcatcta tacctaattt tttggcatta acttgtaaat aagttagtgc
      481 ggttgttgca ctttctttca ttacatttcc taagttgccg gtaagtttta attctccttt
      541 accttcactt agtgcaactt caataaataa tatatctcca ccaacataag tccatgcaag
      601 tcctacagca acacctggta agtttattgc tttgtatata tcattattat aacgtggtgt
      661 gcctaagatt ttttctatat ctgcattagt cacactgggc tttaatttat tttttgtagc
      721 aagttctttt gcaccatatc tcataatact tgctaattgc ctatctaatt ctcttacacc
      781 actttctctg gtatggtttt ctataatttt ttgaagaact gcatcggaaa tttttaagtt
      841 tatttttcct agcccgtgtg cttctttttg ttttggaatt aagtgtgctt ttgcaatttc
      901 aattttttct tcaatagcat aaccacttaa atctattatt tctaatcggt ctcttaatgc
      961 aggttgaatg ctaccaatac tattggcagt agcaataaaa agcactttac ttaagtcgta
     1021 ttctaattct aaataattat cataaaaaga attgttttgt tcggggtcta aaacttctaa
     1081 tagagcagat gatgggtctc ctttgtggtc attgcctact ttatctattt catctaatat
     1141 cattacagga ttgccagatt ttacttttcg taggttttgt aaaattcttc caggcatggc
     1201 tcctatatat gtttttcggt gccctctaat ttcactttca tcatgtaagc cacctaagct
     1261 aactcgggta tattttctac caacagcatt ggctatactt ttacctaagg atgttttacc
     1321 aatacctggg gggcctacaa aacaaagaat agggcttttc atgtctccct ttaattttaa
     1381 tacagcaagg tattctagta ttctgttttt aattttatac atgccgtaat gttctttgtc
     1441 taaaaccttt tttgcatttt ttaaatcata attatcagca gtataatcct cccaaggcag
     1501 gtctaacatt aaattacaat gattgtatat tacagagtag tctggagtgc tggggtgcat
     1561 tctttcaagc tttgcaatat tagagttaaa taattcttta gctgcattag cccatttttt
     1621 tgtttcggcc tttttcttca tttcagcaac ttctttttca ttatgatctc cgcccagctc
     1681 atcttttata ctttttagtt gctgctgtaa aaaatattct ctctgttgtt tgtctaattc
     1741 agttcgggtt ttgttggtta ctttgttttt taattctgca aactgtaatt cacgttgtaa
     1801 aagctgaatt aatttctctg ctcttatagt aatatcattc atttctaaca aagcctgttt
     1861 ctctgctaat tcagtatttt gattactgct tacaaagttg atgagaaaag aagggctctc
     1921 aatattcttt aaaataatgc tagcctcagt tggcaggttt ggagatagtt gaattatttg
     1981 tgttgccaaa tctttgatag aatcaaccat tgcatcaaag ttttcagtat ttggtttttc
     2041 gtcttctttt aaaatattaa tttttgcttt aaaataagga tccactttag taatacttat
     2101 taattcaaat ctttttttcc cttgaattat aatagtggtt ccaccatcgg gcattttaat
     2161 taattttaca attttggcaa cagtaccaat attgcataag tcttttgcct ctggttcctc
     2221 aatactgcta tctttttgag caagtacacc aattagttta tcagctttat aagcatcggt
     2281 tactgcttta atacttttat ctcttcctac agtaatgggt aaaacaactc caggaaataa
     2341 aacagtattt cttaatggca atataattaa ctcttcgggt atttttaaat tggcatcttg
     2401 ttcagagtca ttctctccaa taggaataat gggcataaaa tctacttcct cgtcgctatt
     2461 taataagtta gtttttatca taatatattt aattgagcta tttttaattt tctattaatt
     2521 ttaaaataag aaataacaat tatcaatatt aatgccattg gttttttata taaaaatgct
     2581 gtacaaaatg gcataattat gttagtattg cactactttt taagacaaaa agcgttattt
     2641 gaatattttt ttaaattaac tattgttatt tccataaaac cccctacttt tgccgtcccg
     2701 aaaaaataga gttatggcta gagtatgtca aattacaggg aagaaacctc ttacaggaaa
     2761 tagtgtttct cactcaaaca ttaaaactaa gcgtaggttt ttacctaatt tacaaaccaa
     2821 acgtttcttt tttactgaag aaaaccgttg gataacatta aaacttacaa cagaaggtat
     2881 aagaaccatc aacaaaaatg gcttagctac tgttattaaa gatttaagag cacaaggaga
     2941 aaaaatttaa acctaaaagg ttttaatagt atataaattt tattaaaatg gcaaagaaag
     3001 gcaacagggt acaagttata ttggaatgta cagagcataa aacttctgga aaaccaggca
     3061 caagccgcta tataactgtg aaaaataaaa aaaatacccc tgaaagatta gagttaaaaa
     3121 aatttaaccc tattttaaag aaagtaacat tacataaaga aattaaataa tcatggcaaa
     3181 agcagcttca aaaaactcaa aggtaaaaga ccttaaagct tcggcagaag ctaaaaactg
     3241 gactaaagta attaaatctg tacgtagtcc taaaacaggt gcatacactt ttaaagaagc
     3301 cattgtgcac aaagacaaag taaaagacta tttagcttct aaataatttt ttatcaatta
     3361 tagtatttct aataaaggct ttctgtaaag tacagaaggc ttttttagtt tcttatttaa
     3421 ctaaatttgc ttactgagaa aaagtagcga attatacaaa acacaattct tttttacatt
     3481 tcagaatctt cataattatg ggctttttta ataaactttt tggcaaaaaa gaaaaagaaa
     3541 ctttaaacga tggtttgcaa aaaactaaag aaggtttttt tacaaaatta acgaaagcaa
     3601 ttgcaggcaa aagtactgtt gatgcagaag ttttagataa tttagaagag gcattaatag
     3661 gtgctgatgt aggcgttgaa acaactttgc aaattattga gggtattgaa aaaagagtga
     3721 aagaagaaaa atatataagt acagcagaat tgaataaagt attgcagaat gaaattcaaa
     3781 aaatgcttac cgatgctaat gatgatgctt atagaaactt cgaaattcct accaataaaa
     3841 aaccatatat cattttagtt gttggggtta atggtgttgg taaaactacc acaattggta
     3901 aattggcaca taattataag aaagcaggat acaatgtatt attaggtgca gccgatactt
     3961 ttagagctgc agctgtagat caacttacta tatggagtga acgtgttgga gtgccaattg
     4021 taaaacaaga aatgggaagt gacccaagtg ctgtagcatt tgatacagta caaagtgcca
     4081 ttgcaaaagg tagcgatgtt gttttaattg ataccgcagg aaggctgcat aataaaacac
     4141 atttaatgga agagttaagc aaaataaaaa gagtaataaa gaaaaatatt tcagatgcac
     4201 cacacgaagt aatgctggtt ttagatggtt ctacaggtca aaatgcttta gaacaagcaa
     4261 agcaatttac tgcagctacc gatgtgaatg ctcttactat aactaaatta gatggtactg
     4321 caaaaggagg cgtagtactt gctattgcca accaatttaa aatacctgtt aagtttatag
     4381 gtgtaggcga aaaaatagac gacttaatca tttttgataa acaagaattt gtggatagtt
     4441 tatttaaact tgaaaactaa aaaaatacct ttcacacatc tttaagaaat tttaatgaaa
     4501 gcaaaatcgc taaaacaaga taaagtaaat attataacat taggttgtag taaaaacctg
     4561 gttgatagtg aagttttaag cggccagctt ttagccaatg aaatagatgt agtacacgaa
     4621 aataaaaaac tagatcataa cattgtagta attaatacct gtggttttat tgataaagct
     4681 aaggaagaaa gtatcaatac aattttagac cagcttgaat taaaacgaag aggtaaatta
     4741 gataaagtgt atgttacagg ctgcttaagc gaaagataca gagaagacct tgctaaagaa
     4801 attgctgagg tagatgcatg gtttggtaca ttagaattgc cattaatttt aaaacaattt
     4861 aatgccgatt ataaaactga attattgggc gaaaggcttt taagtacacc aaaacattat
     4921 gccttcatga aaattagcga aggctgtaac agaacttgta gcttttgtgc aataccctta
     4981 atgcgtggca accatattag taaaccaatt gaagttttag ttaaagaagc tgaaacactt
     5041 gtacagaaag gggttaaaga agtaatgtta attgctcaag agcttactta ctatggttta
     5101 gatatttata aaaaacgttc gttgccaaaa ctattaaatg cattagcaga tgttaaagga
     5161 ttagagtgga ttagacttca ttacgcatat ccttccaaat ttcctcttga aatagttgag
     5221 gtaatgaaag aaagagaaaa tatttgtaat tatttagata tgcctttgca acatgcaagt
     5281 gatgcaatgt tgaaggcaat gagaagaaat attacaagag aggaaatgaa tgaattaatc
     5341 tataaaataa gaaacattat cccagatatt tgcttacgta caacattaat ttcaggattt
     5401 cctggcgaaa cagaaaagga tgtagaagag ttaaaagatt ttttaaaaat tcaccgcttt
     5461 gacagggtag gaatatttac ttatagccat gaagaaaata caggagctta tactttagaa
     5521 gacacaattc cgcaaacaga aaaagaagct agagcacaag aaataatgga agtgcaacaa
     5581 gaaataagtt tagaaaaaaa tcaagctaaa gttggtaaaa catttaaagt tattattgat
     5641 aaaaaagaag caggaagata tttaggtaga actgaatttg atagtgtaga agtagataat
     5701 gaagtagtaa tacaatcaga taagaaactt acaataggaa gttttgtaaa tgtaaaaatt
     5761 actaaagcct acgattatga tatagaagga gtagtggttg aataagcata aataatgtaa
     5821 aaaattactc tttgaaattt aaaaccaact ttaattgttc tatgatattg taaataattg
     5881 gacaccgatt atagtgcata aaagtagtaa aaagcctatc cttaaaatta ggcaaatgca
     5941 tagcaggaaa ttctttacat ccttcaaacc taaaattata aacagtacat ttattatctt
     6001 ttaaaaaact acatggtaca gttttaatta acatcatacc attagagcct ttttctataa
     6061 attgttcatc aaatattgct ctgttaattt ctaaatgtgc agctaaatta tttgcttcaa
     6121 tttcttgcac gtttattaaa agtgttttgc aacaatttcc gcacaaagtg caatcaattt
     6181 ttggagatac tatttcattt aaaagatgta cttgtttatc aatatattct ccatcaaaac
     6241 tctttaaata agaaataaaa tcatcgtttt ctttttcttt attactagct aattgctgaa
     6301 tatttgtaag atttgtttca aaatttaaag acatataaac aaacttatgt tatttatttc
     6361 atttgcctgc aaatccttgt tttatccaca tttcattggc ttgtgtttat aatctatact
     6421 ttagatgcct taatgctttg ttagatttgt aactaagaaa actataaaaa atagcaacaa
     6481 tttttcaaat gagtgaaatt aaaaaaatga caaattatac cgaagatgat attaaaggtt
     6541 tagattggaa agagcatata agattaaggc ctggtatgta cattggtaaa ttaggagatg
     6601 gctcatctgc tgatgacggt atttatgttt tattaaaaga agttttagat aattgtgttg
     6661 atgaacatac aatgggtttt ggaaagcata ttgatatttc tatagaaaat aaaactgtaa
     6721 ccatcagaga ttttggtaga ggcattccat taggcaaggt tgtagatgtt gttagtaaaa
     6781 taaatacagg agcaaagtac gatagtaaag catttcaaaa aagtgttggt ttaaatggcg
     6841 ttggtacaaa ggctgtaaat gc
//
LOCUS       DAOQPK010000106         6821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_6359_length_6821_cov_4.492167, whole genome shotgun sequence.
ACCESSION   DAOQPK010000106 DAOQPK010000000
VERSION     DAOQPK010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6359_length_6821_cov_4.492167"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..1389)
                     /locus_tag="PLU36_06280"
     CDS             complement(139..1389)
                     /locus_tag="PLU36_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMZ46394.1"
                     /translation="MKNTDTVWFIAGFMLMLDLYAFQAIRVLTIGLHTKTRIVVQGSY
                     WFLSVAAILVLLSIPYLQTLQTSKFFRNYIFAIFVGLFLAKLLGSLFFVIDDIRRLFV
                     WVAGKFNTTKQIAENATNGPSISRSVFLSWLGLGAGATLFGTMLYGFSNRYNYQIKKV
                     SLSFKNLPNSFKGFKIVQISDIHSGSFQNKKAVEKGVEKILEQKPDMILFTGDLVNDS
                     YEEVIDYIDIFSRLKAPYGVYSILGNHDYGDYSTWDTLEAKIENIEKIKQAHSKMGWR
                     LLLNEHVAIEKNSESFALIGIENWGAKAYFPKYGKLQEAYNGTEKYAFKLLMSHDPSH
                     WDAEVRTKYTDIDLMLAGHTHGMQFGIENPYFKWSPVQYMYKQWAGLYEEGNQKLYVN
                     RGYGFIGYPGRVGILPEITVIELT"
     gene            1503..2183
                     /locus_tag="PLU36_06285"
     CDS             1503..2183
                     /locus_tag="PLU36_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HMZ46395.1"
                     /translation="MQFTYYGHSCFSIIINGKHLLFDPFITYNELASHIDIESIKADY
                     IFISHGHADHIADCISIAKRTNALVICSWEIHEWLNKKEISNTHPMNTGGKKVFDFGT
                     VKCTVAQHSSGLPDGSYGGNPMGFHFITKEKSFYYSGDTALTLDMQLIPAWGKPNFAI
                     LPIGDNFTMDYIDAEKAACFVETKVIIGVHYDTFGFIKINTQEAKEYFTSKGKLLLLP
                     TIGETIDV"
     gene            complement(2237..>6821)
                     /gene="sprA"
                     /locus_tag="PLU36_06290"
     CDS             complement(2237..>6821)
                     /gene="sprA"
                     /locus_tag="PLU36_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HMZ46396.1"
                     /translation="LDALSDPRTRQVYTNELFPNLTTNITNVQAFTFDLAYYPTERGP
                     YNYESSPLQVTASGRLLNPKQRWGGIMRNIDQTDFETGNVEYLEFWVQDPFIKTANPT
                     SKGKLVINLGNISEDILKDGKRFYENGLPSPNIPAVVDSSTWGKQPINPIQVTQAFSN
                     DPNDRPYQDVGFDGLNDEGEKNKKSYYLAKLAANFSTSSPLYIQANQDPSNDNYKWYR
                     DASYDAVGADILQRYKNFNNPQGNSPVAGTNNDLSAAATLYPDNEDLNRDNTLNETEA
                     YYEYEVDLSKNMDVGITKYITDKRSINVKYVNGTTGTENWYLFRVPIKEYTSNVGGIT
                     DFKSIRFIRMYLTDFEDSVVMRFARFDLVRNQWRNFNYKLDTAGQYIALNNNTTTFNT
                     LAVNIEENSNRTPVNYVMPPGIERVQQLSNNGVNLLQNEQAMSLRVQGLQDGDARGVI
                     KTLNLDMRQFGKLSMFIHAESMPKFPTVNDNELNAVIRIGQDFLNNYYEIKIPLKITA
                     PGKYNTSDSSAAKVWPDSNRLDFSLQELINLKLRRNASGSPLSTIYREQVGFKTYSVY
                     GNPNLGEVRGILVAVENAKDNNPFDLYSEVWINELRLSQLDERGAYAALGRIDVQLAD
                     LGSLSFSANTYSKGFGTIEQRVNERARDNMFQFDAAASIDAGKLVPKSAKISIPIYAS
                     INKTIKRPEYDPYDKDVKLKDKINSAANSRQRDSIKNTALDQTTIKTINFTNIRVQGN
                     GKNKLWSLSNFDVSYSYTKTEQSSPLVLKNDVTKQRGGFGYTFNNSSKYIEPFKKLLV
                     KRKSNWYNLIRDFNFNYMPSLLSFRADINRQFGEYIPRIVSTDGTGKIERVDTTYDKY
                     FTFDRFYNLRWDLTRSLNFDFSATNNARIDEPEGAIDTKEKRDTIRDKFWSGGRNTLY
                     NQRAVASYTLPLAKLPLTDWVTSRYSYTTTYNWIGASLLALPLGNIIENSQENTFNAE
                     FDFTRLYSKFKILRALDNAPSSTGKNNKVVTPKRTTPTVTLKTKAEVLKGLKGKKRIA
                     ALKKWRQQRRDARIAARLIKQNQPVEMNDVLRTTGKMVTMVKRASINYNETFRSRIPG
                     FMGRANHLGNNLKTMQPGLDFVFGMQPDSNWLNTKAAQGILTRDTLFNTLFRQNFEQR
                     LSVTVQAEPLKELIIDLNLDKSFSKEFTELFKDVSNTNKYQHLNPYAAGGFSVSYISF
                     KTLFQKTNPNEISETFKKFQDYRLIMSKRVAETNPYWNGQYDADGFAKGYGRYAQDVL
                     IPSFLAAYTGKDPKNISLLNQNNKNIRSNPFGGILPKPNWRVTYTGLTRIPALAKKFS
                     TISFSHAYNGTVSMNSYNSALLFADPFRYGAPQFIDTVSGNYIPFFLVPNITIQEQFA
                     PLIGIDVTTLSQLNVKFEFKKSRQLSLSLIDYQLSETNSTEWVVGAGFRKRGLSFIKI
                     KGKALENDINFRLDLSMRDDATTNSRLDQANAYGTGGQKVITIQPSIDYVLNNRINIK
                     LFFDQRRVVPYISTSAPTINTRAGVQIRVSLAP"
BASE COUNT         2116 a         1151 c         1032 g         2522 t
ORIGIN      
        1 aaaattgggc caataatata gttaagaaat atttttttac tcttcttcag taattagttt
       61 ttatattatt acaaattacg aatatactgt tgagattgta aaatagaatt gctatatttt
      121 tttaataaga gagagtaatt aggtgagttc tattacagta atttctggta aaattcctac
      181 tctacctggg tagccaataa aaccataacc acggtttaca tataattttt gatttccttc
      241 ttcgtataaa ccagcccatt gtttgtacat atactgtaca gggctccatt taaaataagg
      301 attttctatt ccaaactgca ttccatgcgt atgccctgca agcattaaat caatatcggt
      361 atatttagtt cttacttcag catcccaatg gctggggtcg tggctcatta ataatttgaa
      421 ggcatatttt tctgttccgt tataagcctc ttgtaatttt ccatatttgg ggaaataagc
      481 tttggcaccc cagttttcaa taccaattaa tgcaaaagat tcactatttt tttctattgc
      541 tacatgttcg tttaacaaca aacgccaacc catttttgaa tgagcttgtt ttattttttc
      601 aatattttct attttagctt ctaaagtatc ccaggttgaa taatctccat aatcatggtt
      661 gcctaaaata ctataaactc cataaggtgc ttttagccta ctgaaaatat ctatataatc
      721 aatcacttct tcatagctgt cgtttactaa atctcctgta aataaaatca tatcgggctt
      781 ttgctctaat attttttcca cgcctttttc tactgctttt ttattttgaa aactgccact
      841 atgtatatcg ctaatttgaa caattttaaa tcctttaaat gaattaggta aatttttaaa
      901 agacaaggaa acttttttta tctgataatt gtatctatta ctaaagccat ataacatggt
      961 tccaaataat gttgcacctg cacctaagcc tagccaactt aaaaatacac ttcttgaaat
     1021 ggatggacca ttggttgcat tttcggcaat ttgttttgtg gtattaaact tacctgcaac
     1081 ccaaacaaac aaccttctta tatcatctat cacaaaaaat aacgaaccaa gtaacttggc
     1141 taaaaacaag cctacaaaaa tggcgaaaat gtaatttctg aaaaacttag atgtttgtaa
     1201 agtttgcaaa tagggtatag ataataaaac caaaatagca gccacactta aaaaccaata
     1261 actaccttgt actacaattc ttgttttagt atgtaggcca atagttaaaa ctcttatagc
     1321 ctgaaaggca tacaaatcta acataagcat aaatcctgca ataaaccaaa ctgtatcagt
     1381 atttttcatg tatataaact tatcaaaaca ttgcaaagaa aaaaatattc aaggtttaat
     1441 ttatttatac ccgaagattt aacattaatt gctgaaattt ggatgtctta aaaaatataa
     1501 ctatgcaatt tacttattac ggccattctt gtttttctat aattataaat ggcaaacacc
     1561 ttttgttcga cccttttatt acctacaatg agttagcaag ccatattgat atagaaagta
     1621 taaaagcaga ctatatattt attagtcatg gccatgcaga tcatattgct gattgtatta
     1681 gcattgctaa aagaaccaat gccttggtta tttgtagttg ggaaatacat gaatggttga
     1741 acaaaaagga aataagcaat acgcacccta tgaatactgg tggtaaaaaa gtttttgatt
     1801 ttggaacagt aaaatgcact gttgcacaac atagcagtgg attgccagat ggcagttatg
     1861 gtggcaaccc aatgggtttt cattttataa caaaagaaaa atcattttac tatagtggcg
     1921 atactgcact tacattagat atgcaattaa taccagcctg gggtaagccc aactttgcta
     1981 ttttacctat tggcgataat tttactatgg attatataga tgcagaaaaa gcagcttgtt
     2041 ttgtagaaac aaaagttatt attggcgttc actatgatac ttttggattt ataaaaatta
     2101 acacacaaga agcaaaagaa tattttacat caaagggtaa attactattg ctacctacaa
     2161 ttggcgaaac catagatgta taaaattaaa aagtggccaa gaaaaagcca ctttttaaga
     2221 ataattattt taatgcctaa ggtgctaaag aaactcttat ttgcacacct gctctggtat
     2281 taatggttgg tgccgatgtt gaaatgtaag gcactactct tctttgatca aaaaagagtt
     2341 taatattaat tctattattt aaaacataat caatacttgg ttgtatagta attacttttt
     2401 gcccaccagt accataagca ttagcttggt ctaaacggct atttgttgtg gcatcatctc
     2461 tcatagaaag gtctaatcta aaattaatat cattctctaa tgccttacct tttattttaa
     2521 taaaactaag accacgtttg cggaaacctg caccaacaac ccattcagta ctgtttgttt
     2581 cacttaattg ataatctatt aaacttaaac ttaactgacg gctttttttg aattcgaatt
     2641 ttacatttaa ctgactaagg gttgtaacat ctataccaat taatggtgca aactgttctt
     2701 gtatggttat gtttggtact aagaaaaatg gaatataatt gccactaaca gtatctataa
     2761 attgtggtgc accatatcta aaaggatctg caaataataa tgcactattg taactattca
     2821 tacttacagt accattgtaa gcatgagaaa aactaatggt gctgaatttt tttgctaaag
     2881 caggaattct tgttaaaccg gtataagtta ctctccaatt aggttttggc aaaatgccac
     2941 caaaaggatt agaacgtata tttttattat tttgatttaa taaagaaata tttttagggt
     3001 ctttacctgt atatgctgct aagaaagatg gaattaaaac atcttgtgca tatctaccat
     3061 acccttttgc aaaaccatct gcatcatatt gcccattcca ataaggatta gtttctgcaa
     3121 ctcgtttact cattattaaa cgatagtctt gaaacttctt aaatgtttct gatatttcgt
     3181 ttggattggt tttttgaaat aatgttttaa aagaaatata ggatacacta aaaccaccag
     3241 cagcataagg atttaagtgt tggtatttat ttgtgttaga tacatcttta aaaagttcgg
     3301 taaattcttt actaaatgat ttgtctaaat taaggtctat aattaattct tttaatggtt
     3361 ctgcctgtac tgttacactt aaacgttgtt cgaaattttg acggaataaa gtattaaata
     3421 aagtatctct tgttaaaata ccttgagctg ctttagtgtt taaccaattg ctatctggct
     3481 gcataccaaa aacaaaatct aatcctggct gcattgtttt taaattattt cctaaatgat
     3541 ttgccctacc cataaagcct ggaattctac ttctaaatgt ttcgttataa ttaatagatg
     3601 ctcgtttaac cattgttacc attttaccag tagttcttaa aacatcattc atttctactg
     3661 gttggttttg tttaattaaa cgtgctgcta ttcttgcatc tcgtctttgc tgtctccatt
     3721 tttttaatgc tgctattctt tttttgcctt tcaagccttt tagtacttct gcctttgttt
     3781 taagagttac cgtaggagtt gttctttttg gggtaactac tttattattt tttcctgttg
     3841 aagatggtgc attatctaat gctcttaata ttttaaactt actgtataaa cgagtaaaat
     3901 caaattcggc attaaatgta ttttcctgac tgttttcaat tatatttcca agaggtaatg
     3961 ccaataagct tgcaccaatc cagttataag ttgtggtata actgtaacga gaggttaccc
     4021 aatctgttaa tggtagttta gcaagaggta aggtatagct tgcaacagct ctttggttgt
     4081 ataaagtatt tcgtccgcca ctccaaaatt tatctcttat agtatctctt ttttctttag
     4141 tatcaatggc tccttctggt tcatcaattc ttgcattatt ggttgcagaa aaatcaaagt
     4201 ttaatgaacg ggttaaatcc catctaaggt tataaaacct atcaaacgta aagtatttat
     4261 cgtaagttgt atctactcgt tcaatttttc ctgttccatc tgtgctaaca attcttggta
     4321 tgtattctcc aaattgccta tttatatcgg ctctaaaact tagcaacgat ggcatataat
     4381 taaagttaaa atctcttatt aaattatacc aattactttt tcttttaacc aataattttt
     4441 taaatggctc aatatatttt gatgaattat taaaagtata accaaagcca ccacgttgtt
     4501 ttgtaacatc attttttaaa actaatgggc tactttgttc agttttagtg taggagtagc
     4561 ttacatcaaa attgcttaaa ctccataatt tgtttttacc atttccttgt acccttatgt
     4621 tggtaaaatt aattgtttta atagtggttt ggtctaaggc tgtatttttt atagaatctc
     4681 tttggcgact attggcggca ctgtttattt tatcttttag ttttacatct ttatcgtaag
     4741 ggtcgtactc tgggcgttta attgttttat tgatactggc ataaattggg atacttattt
     4801 tagcagattt tggtacaagt ttaccagcat caatagaggc tgctgcatca aactgaaaca
     4861 tattatctct tgctcgttca ttaactcttt gctctatggt gccaaaacct ttagagtaag
     4921 tattggctga aaaactaagt gaaccaagat cggcaagttg tacatctatt cttcctaaag
     4981 ctgcataagc tcctctttca tctaactgag aaagccttaa ttcatttatc caaacttccg
     5041 aatataaatc aaaaggatta ttgtcttttg cattttctac tgctactaaa attcctctta
     5101 cttctcctaa attaggatta ccatataccg aataggtttt aaagccaacc tgctctctat
     5161 atattgtact taaagggctg ccagatgcat tacgccttag ctttaaattt attaattctt
     5221 gtaaactaaa atcaagtctg ttgctatctg gccacacttt tgcagcacta gagtctgaag
     5281 tgttgtattt tcctggtgca gttattttta aaggaatttt aatttcgtaa tagttattta
     5341 aaaagtcttg tccaattcta attactgcat ttaattcatt gtcattaact gttggaaatt
     5401 taggcatact ctcggcatga ataaacattg ataatttgcc aaactgacgc atatctaaat
     5461 tcagagtttt tattacgcct cttgcatcac catcttgtaa accttgtact cttaaactca
     5521 ttgcctgttc gttttgtaat aggtttactc cgttattgct taattgttgt actctttcta
     5581 tacctggtgg cattacataa tttacagggg tacgattgct gttttcttca atgtttacag
     5641 ctaaagtgtt aaaggttgta gtattattgt ttagggcaat atactggcct gcagtatcta
     5701 acttataatt aaaatttctc cattggtttc taactaaatc aaaccttgca aaacgcatta
     5761 caaccgagtc ttcaaaatcg gttaaataca ttcttataaa acgaatggat ttaaaatctg
     5821 taattccacc tacattactg gtatattctt taataggaac tctaaataaa taccaatttt
     5881 ctgttcctgt tgttccgttt acgtatttta cattaatgct tcttttatct gtaatatatt
     5941 ttgtaatgcc tacatccata ttttttgata aatctacttc gtattcatag taagcttctg
     6001 tttcgtttaa agtgttgtct ctgtttaaat cttcattgtc tggatataaa gttgctgctg
     6061 cagataaatc gttgtttgtt cctgctacag ggctattgcc ttgtgggtta ttaaaatttt
     6121 tatagcgttg taaaatatca gcaccaactg catcgtaact tgcatctctg taccatttat
     6181 agttatcgtt actggggtct tggtttgctt gaatatataa tggagaactg gtagaaaagt
     6241 ttgctgccaa ttttgctaag taataagatt ttttattttt ttctccctca tcatttaacc
     6301 catcaaaacc aacgtcttgg taaggcctat cattggggtc gttgctaaat gcttgtgtaa
     6361 cttgaattgg gttgataggc tgtttacccc aagtgctgct atctactact gcaggtatat
     6421 ttggtgatgg aagcccattt tcataaaaac gtttcccatc ttttaaaata tcttcactta
     6481 tattacctaa gttaattacc agtttacctt tagaagttgg gttagccgtt tttataaatg
     6541 gatcttgtac ccaaaactcc aggtattcta cattgcctgt ttcaaaatca gtttggtcaa
     6601 tatttctcat aataccgccc caacgttgtt tgggatttaa taatctacca cttgcagtaa
     6661 cttgtaatgg actgctttcg taattgtaag ggccacgttc ggtaggataa taagccaaat
     6721 caaaagtaaa agcctgaacg ttggtaatat tggttgtaag gttaggaaat aattcattgg
     6781 tgtaaacctg ccttgtacgt gggtcgctta atgcatctaa a
//
LOCUS       DAOQPK010000107         6810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_6373_length_6810_cov_5.429460, whole genome shotgun sequence.
ACCESSION   DAOQPK010000107 DAOQPK010000000
VERSION     DAOQPK010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6810
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6373_length_6810_cov_5.429460"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..2084)
                     /gene="gyrB"
                     /locus_tag="PLU36_06295"
                     /pseudo
     CDS             complement(74..2084)
                     /gene="gyrB"
                     /locus_tag="PLU36_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612990.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
     gene            2208..2723
                     /gene="rimM"
                     /locus_tag="PLU36_06300"
     CDS             2208..2723
                     /gene="rimM"
                     /locus_tag="PLU36_06300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HMZ46397.1"
                     /translation="MNNYIHIGKIVATFGVNGEVILNHSLAKKAGFKNITVLFVELIK
                     GNKIPYFLVHAKAKTNNETILLFEDINNKEAAHKLIGKPIWLNEEDFRKQAGKQAPIG
                     LLGYNVVENKNILGSVLEVIEQPHQVLLGILYKEKDAYIPLHQETLIKINHDKKEIIV
                     TLPDGLLDIYS"
     gene            2840..5266
                     /gene="pheT"
                     /locus_tag="PLU36_06305"
     CDS             2840..5266
                     /gene="pheT"
                     /locus_tag="PLU36_06305"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HMZ46398.1"
                     /translation="MTISYNWLSEYLPIKIEPEKLSKILTSVGLEVEFMEPYQSIKGA
                     LEGLVIGEVLSCEQHPNADKLKITTVNINQEQPLQIVCGASNVAIAQKVVVATVGTTI
                     YPLSGDPITMKVAKIRGVESYGMICAEDEIGLSANHNGIIVLPNDVEVGNAAAQYFNP
                     YNDIIFEIGLTPNRMDAMSHYGVAKDVCAYLSHHLKEEVKPKNIFSVPLKSDNNNIPI
                     QVTIENIDACPRYAGVSISGVSIKESPIWLKNKLSAIGLKPINNIVDITNYILHETGQ
                     PLHAFDVNAITNNQIIIKNLDANTSFITLDGIERKLSNNDLMICNGANEPMCIAGVFG
                     GFKSGITPSTTNIFVECACFNASSIRKSSIHHSLRTDAATRFEKGVDIGNTINVLQRA
                     IALIKEIAGGTVSCEIIDVYPVKKEKAEVSLKNHYLKKLSGKNYHPDAVKRILTSLGF
                     EIVKEGMDEIRLAVPFSKPDITLAADIVEEIVRIDGLDNIEIPYSINITPQVTFLETK
                     EGLREKIANLLTGLGFCEILTNSITNSKYFTNNELNNTVKMLNNLSAELDILKPNMLP
                     TGLEVLVFNINRKNCNLQLFEFGKTYCVNSDGNYSEEEHVTLYLTGLESENNWNQKAK
                     TIGFYETKGIANAILKLLNIQDINFTEDASNASFINIATNNTLLGNIQIISKNTLDAF
                     DIKQAVFCVDFNFSALLNEVEKNKIVYKEVSKFPVVQRDLAMVVSSKTTYNQVEQVIK
                     KLKLNTLQSTRLFDIFMSEKLGTDKKSMAVNFTFSDNEKTLTDKDIDGMMNKLIFHFE
                     KELAAEIRKQ"
     gene            5319..5573
                     /locus_tag="PLU36_06310"
     CDS             5319..5573
                     /locus_tag="PLU36_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46399.1"
                     /translation="MDSTIDQQLSVLQNKLQLLLEKCHALKKENELLKKQIEKNKQSQ
                     ENSYKNLIALKTNNPEELEKRIDKYLQEIDRCLLLLNVEA"
     gene            5583..5885
                     /locus_tag="PLU36_06315"
     CDS             5583..5885
                     /locus_tag="PLU36_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HMZ46400.1"
                     /translation="MSELIPANIVIADRTYRLKIDADDEELVRKTAKLINDKILEFKD
                     SLAGKDMQDYVSMVLLWFATEQNKTGKAMISLQTTEQKLLAFEKQIDKAINSINTI"
     gene            5939..>6810
                     /locus_tag="PLU36_06320"
     CDS             5939..>6810
                     /locus_tag="PLU36_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46401.1"
                     /translation="MSLQYYNPFNNLYFNNNTCFLTGEEIEEQQNYITVFPEWVLDRF
                     LLHDKKFTLMDNVTSFNYNQMVLPCSTKVKNAYNLLDQEIKAAFENGYQSVQEIDSQK
                     LFTWMGIFVYCVLYHDLLIEKEKKQKQNVEFDISPTLKNRFALFHLMLQSLVAPISFG
                     AIKPWGITIVKLKYSKDIFNYRDDTVNLMFTLGMNGFGIIATLQDNGIVMQKEQALLH
                     KINQATLHPIQFEELKARFIYNNYLLQFKPKYIIKQHADCLDINAVEFKANDISTLFA
                     NWNEKTFADVLSEY"
BASE COUNT         2366 a         1148 c         1004 g         2292 t
ORIGIN      
        1 aagttggggt accccaactt tatgaagact tacacagttt ataagatagt acctataaat
       61 tggcacttac attttacaca tctagtttag catattttgc attggcttct ataaactctc
      121 ttcttggagc aacctcgtcg cccattagca tactaaacac tctatctgct tctgctgcac
      181 tatcaattgt tactcttttt aaaacccttc tttctgggtc cattgtagtt tcccatagtt
      241 gctctgcgtt catttcaccc aaccctttgt aacgttgtat agtaacagag tcttcttttc
      301 cgttactaag cttttcaaca atttgcactc tttgttcttc attataggca tactcagcct
      361 cttttccttt tttaactaaa tataatggtg gttgtgccaa gtacacgtat ccctgttcaa
      421 ccatttcttt catataacga aatataaatg ttagaattaa agttgcaata tggcttccat
      481 caacgtcagc atcggtcata attattaact tatgatacct tagtttagat aggtttaatg
      541 ccttgggatc ttctggtgtg ccaacagtta cccctaaagc agtgtacata tttcttattt
      601 cttcattttc atatatttta tgttccattg ctttttcaac attcaaaatt ttacctcgta
      661 atggcagtat tgcttggtag cttctgtttc ttccttgctt tgctgtacca cctgcactgt
      721 ctccttctac taaatacaat tcgcattttg ctgcatctct ttcgctacag tctgccagtt
      781 ttcctggtaa accacctcca ctcaaaacag tttttctttg caccaactct ctagcttttc
      841 ttgctgccac ccttgcttgt gctgctaaaa ttacttgcta attacgtttt tggcttcttt
      901 agggttttct tctaaatatg cttgtagtgc tttagataca gaagattgaa caataccact
      961 tacatcgcta ttgcctagtt ttgttttggt ttgtccttca aattgtggct ctggcacttt
     1021 aacagaaatt attgcactta gtccttctct aaaatcatct ccttctactt ctacttttgc
     1081 cttttcaaaa agcccctctt tatcgccata gcttttaaaa acacgtgtta aagctgttct
     1141 aaaaccactt acatgcgtac ccccttctat tgtattaata ttgtttacat agctaaaaat
     1201 atgttcttta aaatcatcgt tataagtcat ggcaacttca acagcaacat tgcttgcagc
     1261 atcatatcct tcaaagtata atgtatttgc aattagtgca tttctattgc tagatttatc
     1321 taacatttca acaaattcaa ctattccgcc ttcactataa aatgttttgg taaaagttgt
     1381 accatcatct aatgtttctc ttaaatcatt taaaataatc gaaacatttc tatttaaata
     1441 agcaagttct cttaacctgc ctgctaaaat atcttttaca tatattgttg tttgaaaaat
     1501 agaagcatca ggccaaaagt gtacttttgt tcctgttatt tcacttgtac caatttctct
     1561 tacagaatat tgtggtacgc caattctata ttcctgttca aatatttttc cctctctttg
     1621 aacagtaacc aacatttttg agcttaaagc attaacacaa cttaccccta caccatgtaa
     1681 gccaccagat actttatagg tatttttatc aaacttacct cctgcatgta atacagtcat
     1741 tacaacttct aatgcacttc tgttttcttt agtatgcatt gctgtcggaa ttcctcgtcc
     1801 atcatcttgt acactaatgc tgttatcttc atgaatggtt acagtaatat ttttacaata
     1861 tcctgctaaa gcttcatcaa tactgttatc aacaacttca taaaccaaat ggtgtaaacc
     1921 ttttacgcta atatcgccaa tatacatagc aggtctttta cgtactgctt ctaatccttc
     1981 taaaacctga atactatcgg caccataatt tgacttttgt tccatgctca taatgcttgt
     2041 gctacttgat ttttattaat ttcaaaatgc ttgcgaatgt acatataaaa ccgctctttt
     2101 tattatcttt ttttatgtat taaaaattaa ttttcaagga atcaaagtag ctgttttata
     2161 gtttaattgg tagtatattc aaattaaata attttgcagt ttgttttatg aataattata
     2221 tccatattgg taaaattgtg gccacttttg gtgtaaatgg agaggtgatt ttaaatcact
     2281 ctttagctaa aaaagcaggc tttaaaaata taactgtact ttttgtagaa ctaattaaag
     2341 gcaataaaat cccctacttt ttagttcatg caaaagccaa aaccaataac gaaaccattt
     2401 tattgtttga agacataaac aataaagaag ctgcccataa gcttataggc aaaccaattt
     2461 ggctaaacga ggaagatttt agaaagcaag ctggaaagca ggcccctatt ggcttattgg
     2521 gttataatgt tgttgaaaat aaaaatattt taggctctgt tttagaagtt atagaacaac
     2581 cccatcaggt tttattgggt attctttaca aagaaaaaga tgcttatatt ccccttcatc
     2641 aggaaacctt aataaaaatt aatcacgata agaaagaaat tattgttaca ctgccagatg
     2701 gattgttaga tatttattcc taaaaaccag ttatttactt tcctttcctt actaaattct
     2761 attctttaaa aaatatattt aatgtgtttt atacatttct ttaaatagta tcttcgctag
     2821 ctgtataagc aaataaatta tgacaataag ctataattgg ctaagtgaat atttacccat
     2881 aaaaattgaa ccagaaaaac tttcaaaaat attaacttct gtaggtttag aagtggagtt
     2941 tatggaaccc taccaaagta ttaaaggtgc attagagggt ttggtaattg gagaagtttt
     3001 aagttgcgaa caacacccca atgccgataa actaaaaatt accactgtaa atattaatca
     3061 agagcaaccc ctgcaaattg tttgtggggc atctaatgtt gcaatagccc aaaaggttgt
     3121 tgtagccact gttggtacaa ccatttatcc ccttagtgga gacccaataa ccatgaaagt
     3181 agctaaaatt cgtggtgtag aaagttatgg aatgatttgt gccgaagatg aaattggttt
     3241 atctgccaac cataatggca ttatagtatt acccaatgat gttgaagttg gcaacgctgc
     3301 tgctcaatat tttaaccctt ataatgatat tatttttgaa attggcttaa cccctaatag
     3361 aatggatgca atgagccatt atggtgtagc caaagatgtt tgtgcatatt tatctcatca
     3421 tttaaaagaa gaggtaaagc ccaaaaacat tttctctgta ccgctaaaat cagataataa
     3481 taacataccc attcaggtaa ctattgaaaa tatagatgcc tgcccaagat atgcaggtgt
     3541 tagtattagt ggcgtttcta ttaaagaaag tcccatttgg ttaaaaaata aattatctgc
     3601 tattggcctt aaaccaatta acaatattgt tgatattact aattatattt tacacgaaac
     3661 cggccagccc ttacatgctt ttgatgttaa tgcaattaca aacaaccaaa ttataataaa
     3721 aaacttagat gctaatacaa gctttataac cttagatggt atagaaagaa aattaagcaa
     3781 caatgattta atgatttgta atggagccaa tgaaccaatg tgcattgctg gtgtttttgg
     3841 aggatttaaa agtggaataa caccctctac aacaaatatt tttgtagaat gtgcttgctt
     3901 taatgcaagc tctatccgca aatcttcaat acaccatagc cttagaacag atgctgcaac
     3961 tagatttgaa aaaggtgttg atattggcaa tacaattaat gttttacaaa gggctatagc
     4021 tttaataaaa gaaattgcag gcggtacggt ttcttgtgaa attatagatg tttaccctgt
     4081 taaaaaagaa aaggctgagg tttctctaaa aaaccattac ttaaaaaaac taagcggaaa
     4141 aaattatcac cccgatgcag ttaaaagaat tttaacaagc ctgggctttg aaatagttaa
     4201 ggaaggaatg gatgaaatta ggctggctgt gccttttagc aaaccagaca taacccttgc
     4261 tgcagatatt gttgaagaaa ttgtaagaat tgatggctta gataatattg aaattcctta
     4321 ttctataaac ataaccccac aagtaacctt tttagaaaca aaagaagggt tgagagaaaa
     4381 aattgctaat cttcttaccg gccttggctt ttgcgaaatt ttaaccaact ccattacaaa
     4441 tagtaagtat tttacaaaca atgagttgaa caacaccgtt aaaatgctta acaacttaag
     4501 tgccgagttg gatatcttaa aacccaatat gctacctaca ggattagaag tgttggtctt
     4561 taatattaat agaaaaaatt gtaacctaca gttgtttgaa tttggtaaaa catattgtgt
     4621 aaacagcgat ggcaattatt cggaagaaga acatgtaacc ctatacctta caggcttaga
     4681 aagtgaaaat aactggaacc aaaaagccaa aactattggt ttttatgaaa ccaagggaat
     4741 tgccaatgca atacttaaac tgttgaatat acaggatatt aattttaccg aagatgcaag
     4801 caatgcatct tttataaata ttgctaccaa taataccctg ttaggtaata tacaaataat
     4861 atctaaaaat acactggatg catttgatat aaagcaagcg gttttttgtg ttgattttaa
     4921 tttttctgcc ttattaaatg aagttgaaaa aaacaaaata gtttacaaag aggtaagcaa
     4981 atttcctgtt gtacaacgag atttggcaat ggttgtaagt tcaaaaacta cctataatca
     5041 agtagaacaa gttataaaaa aattaaaatt gaacaccctt caaagtactc gtttgtttga
     5101 tatttttatg agtgaaaaac ttggtacaga taaaaaatca atggctgtaa actttacctt
     5161 tagcgataat gaaaaaacat taaccgataa agatattgat ggcatgatga ataaattaat
     5221 tttccatttt gaaaaagaat tggcagcaga aataagaaaa caataaacac caactataat
     5281 aaaagtattg aaaactttaa ttaactagat ttgtaaaaat ggattcaaca atagatcaac
     5341 aattatctgt attacaaaat aagcttcaac ttttgttgga aaagtgtcat gctttaaaaa
     5401 aagaaaacga actactaaaa aaacaaatag aaaaaaataa acaaagtcaa gaaaattcgt
     5461 ataaaaactt aatagccctt aaaacaaata atccggaaga gttagaaaaa agaatagata
     5521 aatatttaca agaaatagat aggtgtttat tattattaaa tgtagaagca taatttttta
     5581 aaatgtctga attaattcct gccaatattg taattgccga tagaacctat cgtttaaaaa
     5641 ttgatgccga tgatgaagag ctggtacgaa aaacagctaa actaattaat gataaaattc
     5701 tagagtttaa agactctctt gcaggtaaag acatgcaaga ttatgttagt atggttttgc
     5761 tttggtttgc aacagaacaa aataaaaccg gcaaagcaat gatttctctt caaacaaccg
     5821 agcaaaaact gttggccttt gaaaagcaaa ttgataaagc cattaacagc atcaatacaa
     5881 tttaatctat ctcttttgtt ttaatttttc tctcttttaa atctgttaaa acaaaataat
     5941 gtctttacaa tattacaatc cctttaataa tctctatttt aacaataata cttgtttttt
     6001 aacaggagaa gaaatagagg agcaacaaaa ttatattact gtttttcctg aatgggtttt
     6061 agatcgcttt ctattacacg ataaaaaatt taccctaatg gataatgtaa cctcatttaa
     6121 ttacaaccaa atggtgttac cctgttccac aaaagtaaaa aatgcatata atttattaga
     6181 ccaagaaata aaggctgctt tcgaaaacgg ctaccagtct gtgcaagaaa tagatagtca
     6241 aaagttattt acatggatgg gaatttttgt ttactgcgtt ttataccacg atttattaat
     6301 tgaaaaagaa aaaaaacaaa agcagaatgt agaatttgat atttcgccaa ctttaaaaaa
     6361 caggtttgcc ctttttcatt taatgttaca atcattagta gcacctataa gttttggtgc
     6421 tataaaacca tggggcataa ctattgtaaa actaaaatac tcaaaagata tttttaatta
     6481 tagagatgat acagttaacc taatgtttac acttggcatg aatggttttg gtattattgc
     6541 aaccctacaa gataatggta ttgtaatgca aaaagagcag gcattactac ataaaattaa
     6601 tcaagcaacc ctacatccaa ttcaattcga agaactaaaa gcaagattta tttataataa
     6661 ttatttactg caattcaaac caaaatacat tataaaacag catgccgatt gcctagacat
     6721 taatgctgtt gagtttaagg caaatgatat ttctacttta tttgccaact ggaacgaaaa
     6781 aacctttgca gatgttttaa gtgaatattg
//
LOCUS       DAOQPK010000108         6662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_6586_length_6662_cov_4.025730, whole genome shotgun sequence.
ACCESSION   DAOQPK010000108 DAOQPK010000000
VERSION     DAOQPK010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6662
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6586_length_6662_cov_4.025730"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="PLU36_06325"
     CDS             complement(<1..624)
                     /locus_tag="PLU36_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HMZ46402.1"
                     /translation="MSYLFTSESVSEGHPDKVADQISDALIDNFLAFDAQSKVACETL
                     VTPGQVVLAGEVKSKAYLDVQEIARGVIRKIGYTKSEYMFEANSCGILSAIHEQSADI
                     NQGVDRKKKEEQGAGDQGMMFGYANNETEDYMPLALDLAHKILIELAALRRENKQIKY
                     LRPDAKSQVTLEYDDNNKPVRIDAIVVSTQHDDFDTEAAMLAKIKSDL"
     gene            complement(745..2190)
                     /locus_tag="PLU36_06330"
     CDS             complement(745..2190)
                     /locus_tag="PLU36_06330"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HMZ46403.1"
                     /translation="MKNLLSVFFISIIITSCTKISTTDIGNNLIPPIDGINTLDTIID
                     VQSFNVLADSFRISKYQEMVLGNIDNDPLMGSTKAIINAVFKPSYFPFYFPVSKDSLT
                     LDSVVLVLSYQGLYGDTTNPVHLKVFEISQTSKLKADTVYPVSATMNTAGLLGEKIIS
                     DPRKLADSVKAFSENAKNQIRIPLNNSFGNKLLKQFDSSNAYFSDDQFESIFRGFSII
                     PQAGSNTLLRVALNDTNSKVALYYKYKQRVTGVDTSVITYYRPAGITSGASNNIIRNR
                     TGSQSAQYLTNASNVEDSLLFLQAGLGNYVRIKTPGITGLSNRVIHRAELIMEQEAND
                     VNTDGLFTTPNLFLCAISTHPDSAAYRFYIPKDIVTSGADGLVENLALFGGNAVNATD
                     PNGKKISSYNFNITRYIQSIILGKQSVYDLYLFAPVVDYVYAGYGNTAGVYQISSIPL
                     NSPGVGRVRLLGGNSKQHGRKMRLRLVYSTL"
     gene            complement(2224..3039)
                     /locus_tag="PLU36_06335"
     CDS             complement(2224..3039)
                     /locus_tag="PLU36_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HMZ46404.1"
                     /translation="MSKKRILFIATEMSPYLELTEFAEIVNKLAIKSNESGMEVRCIM
                     PRFGVINERRHRLHEVVRLSGINVSVNDDDYPLQIKVASLPNARLQIYFLENEEFFKR
                     KNIFHDDEDKWFEDNALRTVFFAKGALETVRKFGWPPDIIHCSGWMSGLIPAFIKTAY
                     KREPVFLHSKTIFTIGQNTFEEKIGEELLELALINNSITANDLKAFKDNDNAAMFRGG
                     ATFADAITFGTPDPDVKLGEEFAKVRGKKVLPFKGWDTDLTEYLELYNDLAGK"
     gene            complement(3218..4855)
                     /gene="groL"
                     /locus_tag="PLU36_06340"
     CDS             complement(3218..4855)
                     /gene="groL"
                     /locus_tag="PLU36_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583723.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HMZ46405.1"
                     /translation="MAKQIFFDIEARNKMKKGVDTLSNAVKVTLGPKGRNVVIDKKFG
                     APQITKDGVTVAKEIELEDAIENMGAQMVKEVASKTADLAGDGTTTATVLAQSIISEG
                     LKMVAAGANPMDLKRGIDKAVSLVVENLKGQSQTVGSDSKKIQQVATISANNDETIGK
                     LIAEAFGKVGKEGVITVEEAKGTDTTVDVVEGMQFDRGYISPYFVTNSEKMEAELQNP
                     YILIYDKKISAMKDILHILEKVAQSGRPLLIIAEDLEGEALATLVVNKLRGTIKVAAV
                     KAPGFGDRRKEMLTDIAILTAGTVISEELGHKLEGADLTSLGQAASVTIDKDNTTIVG
                     GKGKKADITARVNQIKAQVENTTSDYDREKLQERLAKLAGGVAVLYVGAATEVEMKEK
                     KDRVDDALAATRAAVEEGIVPGGGVAYIRAIDSLDAKVKGQIADEQTGMAIVRRALEE
                     PIRTLTTNAGIDGSIVVQKIKEGKGDFGFNARTEVYENLFKAGVIDPTKVSRVALENA
                     ASIAGMLLTTEAVIADKPEPKSATPAMPGPDMGGMGY"
     gene            complement(4976..5260)
                     /locus_tag="PLU36_06345"
     CDS             complement(4976..5260)
                     /locus_tag="PLU36_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HMZ46406.1"
                     /translation="MATKKLNVTPLHDRVIVKPAAAEEKTAGGIIIPDTAKEKPQRGI
                     VIAAGAGKKDEPVTVKVGDNVLYGKYAGTEIQIEGQDLLIMRESDILAIV"
     gene            complement(5463..>6662)
                     /gene="glnS"
                     /locus_tag="PLU36_06350"
     CDS             complement(5463..>6662)
                     /gene="glnS"
                     /locus_tag="PLU36_06350"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HMZ46407.1"
                     /translation="FTEMKDGKYVDGEKVLRAKVDMSSPNMHMRDPIIYRIKHAHHHR
                     TGNKWCIYPMYDFAHGQSDSIENITHSICTLEFIPHRELYNWLIQQLAIFPSQQYEFA
                     RLNMTYTVMSKRKLLQLVNEKHVVGWDDPRMPTISGMRRRGYGAAALRDFCDKIGIAK
                     RENLIDIGLLEFCVREELNKTALRRMVVFNPLKITITNFNKEEEMVSIEDNPEDPNST
                     HRMVPFSKEIYIEQEDFMEIPPKKFFRLYPGGRVRLKGAYIIECNEVVKDNEGNITEL
                     QCSYLPESKSGNDTSGIHVKGTLHWVSKAHAVHAEVKLFDRLFHIENVAEAEGDFKDH
                     INPNSLQIIEKVYAEPALLDIDIQKKYQFLRKGYFCIDKDSTKEKLIFNRTVTLKDTW
                     AKEVKKN"
BASE COUNT         2017 a         1257 c          893 g         2495 t
ORIGIN      
        1 caaatcagat ttaattttag caagcattgc agcttctgta tcaaaatcat cgtgttgtgt
       61 agatactaca atggcatcaa ttctaactgg tttattatta tcatcgtatt ctaaagtaac
      121 ctggcttttt gcatcgggac gtaaatattt aatttgcttg ttttctcttc ttaatgctgc
      181 taattcaatc aaaattttat gtgctaaatc taaggccaat ggcatataat cttctgtttc
      241 attattggca taaccaaaca tcataccctg gtctcctgca ccttgttctt ctttcttttt
      301 tctatcaaca ccttggttaa tatctgcact ttgttcgtga atagcagaaa gaataccaca
      361 agagttggct tcaaacatat attcgctttt agtgtaacca atttttctaa ttacaccacg
      421 agcaatttct tgtacatcta aataagcttt agattttact tcaccagcaa gtacaacctg
      481 accaggggtt acaagagttt cacaagcaac tttgctttgg gcatcaaaag caagaaaatt
      541 gtcaattaat gcatcagata tttggtctgc tactttatct ggatggcctt cagaaacact
      601 ttcagaagta aataaataag acatgtatta taattttatt tttaatgatt gcaaatataa
      661 aggttgattt tattttaact aaaaagtatt tagtacatat aattaaatag attttaatct
      721 atacaaaata aaatcaattg aaggctacag agtagaatag actaacctta atctcatttt
      781 tcttccatgt tgtttactgt tccctcctaa taatctaacc ctgccaacac ctggtgaatt
      841 taatggtatt gaagatattt gataaacccc agcagtatta ccatatccag catacacata
      901 atccactaca ggagcaaaca aatacaaatc gtaaacagat tgttttccta aaataatact
      961 ttgaatatat ctggtaatat taaaattata cgatgaaatt ttcttcccat tcggatcagt
     1021 agcatttact gcattacctc caaataaagc aagattttca accaatccat ctgcaccaga
     1081 agtaacaata tcttttggaa tataaaatct ataagcagca gaatctgggt gtgtgctaat
     1141 ggcacataaa aataaattag gagttgtaaa tagcccatct gtgtttacat cattggcttc
     1201 ttgttccata attaattctg ctctatgaat aaccctatta ctcaagcctg ttataccagg
     1261 tgttttaatt ctaacataat ttcctaaacc tgcttgtaaa aacaataaac tatcttctac
     1321 attggaagca ttagttaaat attgtgccga ttgtgagcct gttctatttc taataatatt
     1381 attacttgca ccagatgtaa ttcctgcagg cctgtaataa gtaattacac ttgtatcaac
     1441 ccctgtaact ctttgtttgt atttatagta caatgctact ttactatttg tatcatttaa
     1501 agctactctt aaaagagtat tgctaccagc ttgtggtatt attgaaaagc ctctaaaaat
     1561 actttcaaat tggtcatcac taaaatatgc attactacta tcaaattgct ttaagagctt
     1621 attaccaaac gaattattta aaggaattcg gatttgattt tttgcatttt cgctaaatgc
     1681 ttttactgaa tctgcaagtt tacgggggtc tgagatgatt ttttcaccta ataatccagc
     1741 agtattcata gttgcagaaa cagggtaaac tgtgtctgcc tttaatttac ttgtttggct
     1801 tatttcaaat acttttaagt gaacagggtt agttgtatcg ccatataagc cttgataact
     1861 taatactaat acaaccgagt ctagagttaa actatcctta cttaccggaa aataaaaagg
     1921 aaaataggaa ggcttaaaca ctgcattaat tatggctttt gtactaccca tcaatgggtc
     1981 attatcaata ttacctaaaa ccatttcttg gtacttagaa attcgaaagg aatcggctaa
     2041 aacattaaaa ctttgtacat caataatagt gtctaaagta ttaattccat caatgggtgg
     2101 aattaaatta ttacctatat cggttgtaga aatttttgta caagaggtaa tgatgatgct
     2161 tataaaaaaa acggaaagta aattcttcac gcagtagaat attaaagtaa aatgttgatt
     2221 ttgttattta cctgcaaggt cgttgtacaa ttctaaatac tctgttaaat cagtatccca
     2281 tcctttaaaa ggtaatactt tttttccacg tactttggca aactcctctc ctaatttcac
     2341 gtcagggtct ggtgttccaa aagtaattgc atcggcaaat gttgctcctc ctctaaacat
     2401 ggcagcatta tcattgtctt taaaagcttt taaatcgttt gcagttatgc tattgttaat
     2461 taatgctaat tctaaaagtt cctctcctat tttctcttca aaagtatttt ggccaattgt
     2521 aaaaatggtt ttactatgta aaaaaacagg ttctctttta taggctgttt taataaaggc
     2581 aggtattaag ccactcatcc aaccactaca atgaattata tcaggaggcc aaccaaattt
     2641 tcgtacagtt tctaaagcac ctttagcaaa aaaaactgtt cttaaagcat tatcttcaaa
     2701 ccatttgtct tcatcgtcat gaaatatatt ttttctttta aaaaactctt cgttttctaa
     2761 aaaatatatt tgaagcctgg cattcggcaa cgaggctact ttaatttgta aaggataatc
     2821 gtcatcatta acagatacat tgataccact taatcttact acttcatgta agcgatgcct
     2881 tctttcgttt attacaccaa atcgtggcat aatacagcga acttccatac cactttcatt
     2941 gctttttatg gctaatttat tcactatttc agcaaattca gtaagctcta ggtatggcga
     3001 catttctgta gctataaata aaattctttt tttcgacatc agctcttcta atttaggggg
     3061 tcgtctttat aaggctgcaa aattaagata ttatgctcta atatcaaacg tatttatgat
     3121 attaacagcc ttaaattcat catttttttt aattcttaat caaaaaaccc cgaaatgtaa
     3181 atttttcggg gctttttgtt gtatctgtaa ttttaaatta atatcccatt ccacccatat
     3241 ctggacctgg cattgcagga gttgcagatt ttggttctgg tttatcagca ataactgctt
     3301 cggtagttaa taacatacct gcaatagatg ctgcattttc taaagctact ctacttactt
     3361 tggtagggtc tattacacct gctttaaata agttttcata cacttctgtt cttgcattaa
     3421 aaccaaaatc tcctttgcct tctttaatct tttgaactac aatagagcca tcaatacctg
     3481 cattggtagt taaagttcta ataggttctt ctaatgctct tcttactatt gccataccag
     3541 tttgttcatc tgctatttgt ccttttactt tagcatctaa actatcaatt gctctaatat
     3601 aagcaacacc accacctggt acaatacctt cttctactgc agctcttgtt gcagctaatg
     3661 catcatccac tctgtctttc ttttctttca tttctacctc tgtagcagca ccaacgtata
     3721 atacagctac accaccagct aattttgcta aacgttcttg taatttttct ctgtcatagt
     3781 cagaggttgt attttctact tgtgctttta tttgatttac acgagcagta atatcagctt
     3841 ttttaccttt accgccaaca atagttgtat tgtctttatc aattgttact gatgcagctt
     3901 ggcctaatga agttaaatct gcaccttcta atttatgtcc aagttcttcg ctaattacag
     3961 tacctgctgt taatattgca atatctgtaa gcatttcttt tcttctatca ccaaatcctg
     4021 gggcttttac tgctgctact tttattgtac cacgtaattt atttacaact aaagttgcta
     4081 atgcttctcc ttctaaatct tctgcaataa ttaataaagg acgaccactt tgagctactt
     4141 tttctaagat atgtaaaata tctttcattg cagaaatctt cttatcataa attaaaatgt
     4201 aaggattttg caattcagct tccatttttt cgctattggt tacaaagtat ggagaaatgt
     4261 aacctctatc aaactgcata ccttctacaa catcaacagt tgtatcagta ccttttgctt
     4321 cctctacagt tataacacct tctttaccta ctttaccaaa agcttcagca attaatttac
     4381 caattgtttc atcgttgttg gcagatatag ttgctacttg ttgaattttt ttgctgtcgc
     4441 ttccaactgt ttgcgattgg ccttttaagt tttcaacaac taaagaaact gctttatcaa
     4501 tgcctctttt taaatccatt gggtttgcac cagcagctac cattttcaat ccttcgctaa
     4561 taattgattg tgctaaaaca gttgcagttg tagtaccatc gccagctaaa tcggcagttt
     4621 tagatgcaac ttctttaacc atttgtgcac ccatgttttc aatagcatct tctaattcaa
     4681 tttcttttgc tacggttaca ccatctttag ttatttgtgg tgctccaaat tttttatcaa
     4741 taactacatt acgaccttta gggccaagag ttacttttac agcgttagat aaagtatcca
     4801 cgcctttttt cattttattt cttgcttcaa tatcaaagaa aatttgcttt gccatatttc
     4861 ttactgttta agaattttat ttataattag tattttttgt ttttcaacac cagtacctct
     4921 atgtttcaga agttgcgtcg cacacttgta cttttagtac tttgcttatc gtattttaaa
     4981 caatagctaa aatatccgat tctctcataa ttaaaaggtc ttgcccttca atttgaattt
     5041 cggtacctgc atatttacca tataaaacgt tatcaccaac ctttactgta acaggctcat
     5101 cttttttacc agcaccagca gctattacaa taccacgttg aggtttttct tttgcagtat
     5161 caggtataat aattcctcct gcagtttttt cttctgcggc agcaggtttt acaattactc
     5221 tgtcgtgcaa tggagtaaca tttaattttt ttgtagccat aatcctaaat ttttattttt
     5281 tgtattttat ttaaaaatgt ataaccctat cttcaacaat attgccattg atggaataag
     5341 gtcaaaattt cagttttaat ttaattagct aaaaaattgg cagcgaagat gcagaatttt
     5401 agacaaatag ccgtgtcaaa atttcacata actcattaaa ataaaatgca aagctgtaag
     5461 ttttaatttt tctttacttc ttttgcccag gtatctttta aggttacagt tcggttaaaa
     5521 attaattttt ctttggtact gtccttatct atacaaaaat atcctttgcg taaaaattga
     5581 tacttttttt ggatatcaat atctaataaa gcaggctctg cataaacctt ttctattatt
     5641 tgtaaggaat tggggtttat atggtcttta aaatctccct ctgcctctgc tacattttct
     5701 atatgaaata atctatcaaa taatttcact tctgcatgta cagcatgtgc tttactaacc
     5761 caatgtaatg taccttttac atgaatacca ctcgtatcat ttccactttt actttctggc
     5821 aaataactac attgtagctc tgtaatattt ccttcattgt cttttactac ttcattacat
     5881 tcaattatat atgcaccttt tagtcttact cttccaccag gatataatct aaaaaatttc
     5941 tttggtggaa tttccataaa atcctcttgt tctatataaa tttctttact aaaaggcacc
     6001 atacgatgtg tagaatttgg atcttctgga ttatcttcta tacttaccat ttcttcttct
     6061 ttattaaaat tggttatagt aatttttaat ggattaaata caaccattct tctaagagca
     6121 gttttattta actcttctct tacacaaaat tctaacaaac caatatcaat caaattttct
     6181 cttttagcaa ttcctatttt atcacaaaaa tctcttaaag cagcagctcc ataccctctt
     6241 cttctcatac cactaatagt tggcattctt ggatcgtccc atccaactac atgtttttca
     6301 tttactagtt gtaacaattt tcttttactc attacagtat aagtcatatt taaccttgca
     6361 aactcatatt gttgcgaagg gaagattgct aactgttgaa taagccaatt gtatagctct
     6421 ctatgaggta taaattcaag ggtgcaaatt gagtgtgtaa tattttcaat agaatcactt
     6481 tgtccatgag caaaatcgta cattggataa atacaccatt tattacctgt tctatgatgg
     6541 tgagcatgct taattctgta tataatagga tctctcatgt gcatattggg gctgctcata
     6601 tctacttttg ctcttaatac tttttcacca tcgacgtatt taccatcttt catttcggta
     6661 aa
//
LOCUS       DAOQPK010000109         6637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_6621_length_6637_cov_4.542388, whole genome shotgun sequence.
ACCESSION   DAOQPK010000109 DAOQPK010000000
VERSION     DAOQPK010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6637
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6621_length_6637_cov_4.542388"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..1320)
                     /gene="rocD"
                     /locus_tag="PLU36_06355"
     CDS             complement(88..1320)
                     /gene="rocD"
                     /locus_tag="PLU36_06355"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HMZ46408.1"
                     /translation="MHTNNLNKNTAYFIELEEKYGAHNYHPLPVVLEKGEGIFLYDVE
                     GKKYFDFLSGYSAVNQGHCHPKIIAALIEQSKKLTLTSRAFYNNQLGVYEKYITEYFG
                     YQKVLPMNTGVEGGETAIKLARRWAYTKKGVEENKAIIVFAEGNFWGRTMAAISASTD
                     PSSYQGFGPFMPGFITVPYNDLAALENAFQNKNVAAFMVEPIQGEAGVVVPDDGYLKA
                     VRDLCTKYNVLFIADEIQTGLARTGKMLACQHDNIKPDIVILGKALSGGTIPVSAVLC
                     DDEIMMNIKPGEHGSTYGGNPLACVVAMTALQVLKDENMEENATIMGQLLRDELATLK
                     SPFIKTIRGKGLLNAIVIEHKNPNAAWDLCLELKENGLLAKPTHGDKIRFAPPLIINK
                     NQIIESVSIIHKSLKILN"
     gene            complement(1606..2223)
                     /locus_tag="PLU36_06360"
     CDS             complement(1606..2223)
                     /locus_tag="PLU36_06360"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ46409.1"
                     /translation="MMETEREQHWNNVYATKLPNQVSWTQAIPTIALNYFQLLQLNKQ
                     ASIIDIGGGESTLVDFLVEQGFENITVLDISTIAIQKVKQRLGDKAKKINWIVTDILN
                     FKPTQKYDVWHDRATFHFLTNHEHINKYVSLVNDFVTKHLIVSTFSTNGPNKCSGLQT
                     MQYSADSLAQKFNANFKLMHHQLHQHQTPFNTTQNFLYGCFNRIK"
     gene            complement(2250..4466)
                     /locus_tag="PLU36_06365"
     CDS             complement(2250..4466)
                     /locus_tag="PLU36_06365"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HMZ46410.1"
                     /translation="MKVLLSFLILFFSITIHAQQRLVDFVNPFIGTGGHGHTYPGATI
                     PFAMVQLSPDNGTNGWDWVSGYHYSDSVIQGFSHTHLSGTGVGDLCDISVLPIVNRIA
                     SAEKITSKFSHKQETAKPGYYAVHLKDFDVWAEHTTTIRCGFHRYTFPASKNAAIRFD
                     LGFAINYDRTTECSFKKINDTTFVGYRFSKGWAPNQKIFFAVRVSKPVKNIVIIANKE
                     KIDTNNYTAKDIKAWLEFETNLGEKILMKVGLSSADIEGALESLNEINGWNFDLVKQN
                     AENIWERELRKLQIRTNDIELKQNFYTALYHTFLAPNRFSDRYGNYKSANGNIEKGKS
                     IFTVQSLWDTFRAANPLLTITQTELVPAIINSYLTFYDQYGLLPVWELQFNETNCMTG
                     YHAVPIIADAILKGITGFDYNKAYEAMKVSANQNIRAADFYRHFGFVPADKAGQSVTI
                     TLEYAFDDWCIAQVAKRLGKSADFREFRRRSMNWRNVFDKKTGFARAKLSNGNWVTPF
                     DPFHAEHDPEKTPYTEGNAWQHTWFAPHDVYALINYMGGAKKFEIRLDSLFKADSKLT
                     GSHVPPDISGMIGQYVHGNEPSHHIAYLYNYIGKPYKTVDKVKEICTTLYHNKPDGLP
                     GNEDCGQMSAWYVFSVLGFYPVNPASGEYVLSVPLVQESALQLPDGHLFRVIVKEANE
                     KNKYIKQATLNGNPYTKSFLKHSDIMKGGILEITLADKPGTSFGTAMEDLPGRTAK"
     gene            complement(4587..5009)
                     /locus_tag="PLU36_06370"
     CDS             complement(4587..5009)
                     /locus_tag="PLU36_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HMZ46411.1"
                     /translation="MIKLQVIGNLGKDATVNNVNGKNVINFNMAHTERYKDAQGNQKD
                     RTIWVECAYWTDKTAIQPYLKKGTQVYVEGNPDVRTYQTQDGKNGASLTLRIQNIQLL
                     GTRSSDNTSAVQNNTPPQQNTNFTPTSDMNSVADDLPF"
     gene            complement(5139..>6637)
                     /locus_tag="PLU36_06375"
     CDS             complement(5139..>6637)
                     /locus_tag="PLU36_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HMZ46412.1"
                     /translation="YDPTLDLRQYQYPSLDLLETHGSEKIIHDPTELETNKNQIIATL
                     KNYDISIQRISATVGPTVTLYEIVPAPGVRISRIKNLEDDIALSLAALGIRIIAPIPG
                     KGTVGIEVPNVRKTVVSMKTLLNSDKFQNNNYALPIAIGKKIDNDNFIVDLTAMPHLL
                     MAGATGQGKSVGLNAVLVSLLYKKHPSQLKFVLVDPKKVELSLYRIIEKHFLAKLPGE
                     EESIITDTKKVVHTLNALCIEMDNRYDLLKEAGCRNIREYNEKFIHRKLNPQKGHQYL
                     PFIVLVVDEFADLIMTAGKEVEMPIARLAQLARAIGIHLIIATQRPSVNIITGTIKAN
                     FPARIAFKVSSKIDSRTILDTGGAEQLIGKGDMLISYNGDVTRLQCAFVDTPEVENVC
                     EFIGKQLGYPDAFLLPEYVDEKEEAGKEFDADDRDILFEDAAKLIVQNQVGSTSLIQR
                     RMKLGYNRAGRLMDQLERAGIVGPNLGSKAREVKFKSETELDDFLQSI"
BASE COUNT         2019 a         1125 c         1020 g         2473 t
ORIGIN      
        1 ataagtttta tattttggtt gatgatataa aaagctaaaa taattattaa ttgtattata
       61 aactaaagta aaaataattt tcgattttta gtttaatatt tttaatgatt tatggataat
      121 agatacactt tctataattt gatttttatt aattattaat ggtggggcaa acctaatttt
      181 atcgccatgt gttggctttg ccaacaaacc attttctttt aattccaaac aaaggtccca
      241 cgctgcattg gggtttttat gttctattac aattgcattt aatagccctt taccacgaat
      301 ggtttttatg aaaggggatt ttaatgtagc taattcatct cttaataact gtcccataat
      361 ggttgcattt tcttccatat tttcatcttt taacacttgt aatgcagtca ttgccactac
      421 gcaagctaat gggtttcctc cataggtact gccatgttct cctggtttta tgttcatcat
      481 aatttcatca tcacacaata ctgcactaac aggtatggtg cctccactaa gggcttttcc
      541 taaaattaca atatctggtt ttatattatc atgctggcaa gccaacattt ttccggtacg
      601 tgccaaacct gtttgtattt cgtcggcaat aaataaaaca ttgtatttag tgcataaatc
      661 tcttactgct tttaagtacc catcatctgg tactacaaca ccagcttcgc cttgtattgg
      721 ctctaccata aaagctgcta catttttatt ttgaaaagca ttttctaatg cagctaaatc
      781 attatagggt actgtaataa aaccaggcat aaatggacca aagccttgat agctacttgg
      841 gtcggtagaa gctgaaatgg cagccattgt tctaccccaa aaattacctt cggcaaatac
      901 aataattgcc ttattttctt ctactccttt tttagtgtaa gcccatcttc gagcaagttt
      961 tattgcagtt tcgccacctt ctacacctgt attcataggc aatacttttt ggtaaccaaa
     1021 atattcggta atatattttt catatacccc taactggtta ttgtaaaagg cacgtgaggt
     1081 aagggttagt tttttagatt gctctataag agctgcaata atttttgggt ggcaatgtcc
     1141 ttggttaact gctgaatagc cacttaaaaa atcgaaatat ttttttcctt ctacatcgta
     1201 taaaaaaata ccttctcctt tttctaaaac aacaggtagt gggtgataat tatgagctcc
     1261 atatttttct tctaattcta taaagtaagc tgtattctta tttaaattat tggtatgcat
     1321 tttatatttt ttctaattag tttatgaata aaaaataatt tctggtacaa taaattctct
     1381 actaaaatat ttgtgtaatt gaagtaagta attgtaatat gtacttaata gagtagttaa
     1441 ctatattata gatggtctaa aaaatctaaa taaaaagata aaaatttcat gatagttttt
     1501 aaaagtgcaa gatactgaat tagtgttgta gaataaaatg aaattgcttg gctttatttg
     1561 ctattaaata cttaggttta atgtataacc cccaatagca tacttctact taattctatt
     1621 aaagcaacca tataaaaaat tttgggttgt attaaaaggt gtttgatgct ggtgcagttg
     1681 atggtgcatt aatttaaaat ttgcattaaa cttttgggct aaactatctg ccgaatattg
     1741 cattgtttgt aaaccactac atttgtttgg gccattggtt gaaaaggtac ttactattaa
     1801 atgtttggtt acaaaatcat ttacaagcga tacatatttg ttaatatgtt catgattagt
     1861 aagaaagtga aaagtagctc tatcgtgcca cacatcgtat ttttgagtag gtttaaaatt
     1921 tagtatatct gttactatcc aatttatttt ttttgcttta tcgcctaacc tttgttttac
     1981 tttttgaatt gcgatagttg aaatatctaa aacagttatg ttttcaaacc cttgttctac
     2041 taaaaaatca accaaggtgc tttctccacc tcctatatct attatgcttg cttgtttatt
     2101 taattgtaat aattgaaaat aatttaatgc aatagttgga atggcttgtg tccaacttac
     2161 ttgatttggc aattttgttg catatacatt attccaatgt tgttctcttt ctgtttccat
     2221 cataacatca tactacataa tacggtttat tattttgcgg ttctgccagg aaggtcttcc
     2281 atagcagttc caaagcttgt acctggttta tctgctaaag taatttctaa aattcctcct
     2341 ttcattatat ctgaatgttt taaaaaggat ttggtgtaag gattaccatt aagagtagcc
     2401 tgttttatgt atttgttttt ttcatttgcc tcttttacaa ttactctaaa taaatgtcca
     2461 tcgggcaatt gtaatgcact ttcttgtact aatggaacac ttaaaacata ttcgccactt
     2521 gcagggttta cagggtaaaa tcctaaaaca ctaaatacat accaagcact catttgccca
     2581 caatcttcat tacctggtaa gccatctggt ttgttatggt aaagagtggt acaaatttct
     2641 tttactttat ctactgtttt atatggttta ccaatatagt tatacaagta agcaatatgg
     2701 tggctgggtt cgttgccatg tacatattgc ccaatcatac cacttatatc tggtggtaca
     2761 tgtgagcctg taagtttaga atcggctttg aataaagagt ctaatcttat ttcaaatttt
     2821 ttagctccac ccatataatt gattagtgca taaacatcgt gtggtgcaaa ccaggtatgt
     2881 tgccaggcat ttccttcggt gtaaggtgtt ttttctggat catgttctgc atgaaatgga
     2941 tcaaaagggg taacccaatt gccattactt aattttgcac gggcaaagcc tgttttttta
     3001 tcaaatacat ttctccaatt catactacgt cttctaaatt ctctaaaatc agccgatttt
     3061 cctaaacgtt tagctacttg tgcaatacac caatcatcaa aagcatattc taaagtaata
     3121 gttacacttt gccctgcttt atctgctgga acaaagccaa aatgcctata aaaatctgca
     3181 gctctaatat tttggttggc acttactttc attgcttcat aagctttgtt ataatcaaag
     3241 cctgtaatac cttttaaaat tgcatcggca ataatgggca cagcatggta gcctgtcata
     3301 caattggttt cgttaaattg taattcccat acaggcagca agccatattg atcgtaaaaa
     3361 gttaaataag aattgatgat tgccggaact aattctgttt gtgtaattgt tagtaatggg
     3421 ttagctgctc taaatgtatc ccataaagat tgaacagtaa agattgattt tcctttttct
     3481 atatttccat tggcactttt ataattacca tatctatcac taaatctatt gggtgctaaa
     3541 aaagtatggt ataaagcggt atagaaattt tgttttagtt ctatatcgtt ggtacgaatt
     3601 tgtaattttc ttaattctct ttcccaaata ttttcggcat tttgtttaac taaatcaaag
     3661 ttccaaccgt ttatttcatt taagctttct aaagcacctt caatatctgc actgcttaaa
     3721 cctactttca ttaaaatctt ttcgcccaaa tttgtttcaa actctaacca tgctttaatg
     3781 tcttttgcag tataattgtt tgtgtctatt ttttctttgt tggcaataat tacaatattt
     3841 tttacgggct tacttactct tactgcaaaa aatatttttt gattgggtgc ccagcctttg
     3901 ctaaaacggt agcctacaaa agtggtatca ttaatttttt taaaactaca ttctgtagta
     3961 cggtcgtagt taatagcaaa acctaaatcg aaacgaatgg ctgcgttttt ggatgcagga
     4021 aaagtataac gatgaaagcc acaacgaatg gttgttgtat gctcggccca tacgtcaaaa
     4081 tcttttaaat gtacagcata atatcctggt ttggcagttt cttgcttatg agagaatttg
     4141 ctagttattt tttctgccga tgcaattcta tttacaatag gcaaaacaga aatatcacat
     4201 aaatcgccaa cacctgtacc acttaaatgg gtatgactaa acccttgtat tacactatcg
     4261 ctataatggt aaccactcac ccaatcccag ccattggtgc cattatctgg gcttaattga
     4321 accattgcaa aaggtattgt tgcaccaggg taagtatgcc catgaccgcc tgtgccaata
     4381 aagggattca caaaatctac taaccgttgt tgagcatgga tagttatgga gaaaaataaa
     4441 attaaaaaag atagtaaaac tttcatgaat ttatttattc tttataattt attaattaaa
     4501 aaatcaattt agtagaagca gttgttcaaa aaattggttg atgtttttca tcaaccaatt
     4561 gtatatttta aaataaaaaa gtttacttaa aatggtaaat catcagcaac tgaattcata
     4621 tctgatgttg gtgtaaaatt tgtgttttgt tgtggtggcg tattattttg aacggctgaa
     4681 gtattgtctg aacttcttgt gcctaataac tgaatatttt gaattcttaa agttaaagaa
     4741 gcaccatttt ttccatcttg ggtttggtaa gttctaacat cagggtttcc ttctacataa
     4801 acttgtgtgc ctttttttaa ataaggttga atagcagttt tatctgtcca gtaagcacat
     4861 tctacccaaa tggttctatc tttttggttc ccttgtgcat ctttatacct ttcggtatgt
     4921 gccatgttaa agttaattac atttttgcca tttacattgt ttacggtagc atcttttcca
     4981 agattgccaa ttacttgtag cttaatcata acagttcgtt ttggtaaatt gagttaatat
     5041 aaaatttaag aactgccaag atagttaatt aaggtataaa ccaagaaaga tttatactat
     5101 tttatccact tgagaaaaat gattgcagta aatttatttt atatagattg taagaaatcg
     5161 tctaattctg tttccgactt aaatttaact tctcttgctt tgctacctaa attaggccca
     5221 acaatacctg cacgttctaa ttggtccatt aatcttcctg cacggttata tcctaatttc
     5281 attcttcgtt gtatgagaga ggttgagcca acctgatttt gtacaattag cttagctgca
     5341 tcttcaaaca aaatatctct atcatctgca tcaaattctt ttcctgcttc ttctttttca
     5401 tctacatatt ctggtaataa aaatgcatcg gggtagccaa gttgttttcc aataaattca
     5461 catacatttt ctacttcagg cgtatccaca aaggcacatt gcaaacgagt aacatctcca
     5521 ttataactaa ttaacatatc tcctttacca attaattgct ctgcaccacc agtatctaaa
     5581 atagttctgc tatctatttt agaagaaact ttaaatgcaa tacgagctgg aaagtttgct
     5641 ttaatggtac ctgtaataat gtttacagat gggcgttgtg ttgctataat taaatgaata
     5701 cctattgctc ttgccaattg tgccaaacgt gcaattggca tttctacttc tttgccagca
     5761 gtcataatta aatcggcaaa ttcatcaact actaaaacaa taaatggtaa atattgatgc
     5821 cctttttgtg ggtttaattt tctatgaata aatttctcgt tatactctct aatatttcta
     5881 caacctgctt cttttaataa gtcgtacctg ttatccattt ctatacacaa agcatttaag
     5941 gtatgtacta cttttttagt atcggtaata atactttctt cttcgccagg aagttttgcc
     6001 aagaaatgtt tttctataat tcggtataaa ctaagttcta cttttttagg atctactaaa
     6061 acaaatttta gttgcgaagg atgtttttta tacagtaacg aaactaaaac agcatttaaa
     6121 ccaacagatt taccctgccc tgttgcacct gccattaata aatgtggcat agcagttaaa
     6181 tcaacaataa aattatcgtt atctattttt ttaccaattg caattggtaa agcataatta
     6241 ttattttgaa atttatcgct gtttagcaat gttttcatgc taacaacagt tttgcgtaca
     6301 ttgggtacct caatacctac agtgcctttg cctggtattg gtgcaataat acgaatgcca
     6361 agtgctgcaa gacttaaagc tatatcgtct tctaaatttt ttattctgct aattcttaca
     6421 cctggggctg gtactatttc gtataaagta actgttgggc caacagtggc agaaatacgc
     6481 tgaatggaaa tatcgtaatt tttaagagta gcaataattt gatttttgtt tgtttccagt
     6541 tcggttgggt catgaataat tttttcgcta ccatgtgttt ctaataaatc taaactaggg
     6601 tattgatact gtcttaaatc taaagtgggg tcataat
//
LOCUS       DAOQPK010000110         6618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_6643_length_6618_cov_4.250495, whole genome shotgun sequence.
ACCESSION   DAOQPK010000110 DAOQPK010000000
VERSION     DAOQPK010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6618
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6643_length_6618_cov_4.250495"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..1190)
                     /locus_tag="PLU36_06380"
     CDS             complement(36..1190)
                     /locus_tag="PLU36_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMZ46413.1"
                     /translation="MHLQLSEEHLMIQKAARDFAVNECLPGVIERDEHQRFPKEQITQ
                     LAELGFMGMMVKPEYGGSGMDTISYVLAMEEVSKIDASVSVCMSVNNSLVCYGLQAYG
                     NEEQKQKYLTPIAQGKKDGELYIGAFLLSEPEAGSDATSQRTIAEDKGDYYLLNGTKN
                     WITNGSSASVYLVMAQTDVSKGSKGINCFIVEKKWAGVTVAAKENKLGIRGSDTHTIM
                     FTDVKVPKENRIGADGFGFTFAMKTLAGGRIGIASQALGIASGAYELALKYAQERKAF
                     GKEIAKHQAIQFKLADMATRIEAARLLCLKAAWEKDNGMDYALSSSMAKVFASETAMW
                     VTVEAVQIHGGYGYVKEYHVERLMRDAKITQIYEGTSEVQRIVIGRSILK"
     gene            1357..2856
                     /locus_tag="PLU36_06385"
     CDS             1357..2856
                     /locus_tag="PLU36_06385"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HMZ46414.1"
                     /translation="MGIVRKQSIASSIYIYLGFAIGAFNVLFLFPKYLTPAEFGLTRV
                     LLDVSLLIAMFCTLGSCPAVLKFYPFYHSYLPKKKIEIPFFTILLTILGCIIFILIAD
                     GSKSYIVRKFGEKSPLFNEYYYLIYPLSISYAFWYLFEASSWSIKKTVLPNFLKEVGF
                     RIITTILVALYIVKLISFKQFISLFSLMYIVPVIILLYALLKDNFFVITFNISSVTKR
                     LWKQITLFSLFVFTGQTLNVIARTSDTIILASQSKNGLADAAVFTVVTYLVTLMDVPM
                     RGMTGIASSVIAYAWKDKDMNKIKEMYQKTALNLLIVGIGIFGILTINMFNFTQHFGT
                     NYSLLPTLLLILGFAKLIDLATGMNAQILLSSKYWRIDFITSMGFVLISVPLNIFLIK
                     KYGLIGSAYANLISVFIYNTTRLLIIWKLFKLQPYNHKNLLTMVIAAVCFFIVYISPS
                     LNNIYADAILKTVVFMPLYIFLILKLKISEDFNQLLLSFIEKIKIRTKK"
     gene            2856..3560
                     /locus_tag="PLU36_06390"
     CDS             2856..3560
                     /locus_tag="PLU36_06390"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ46415.1"
                     /translation="MKNTIISFIPPILIELGKKTGILKDKSKVIIYKKNENDTQDLDM
                     YFDPEYAKILDSWGEKTTWIEIQMLLANCKGKVLDIACGSGVTMRILNKYKDLDVHGC
                     DISDLLIKKAVSLGIAENNLKVTDATKMDYADLAFDYSYSIGSLEHFTEKGISEFIAE
                     AARVTKYTSMHMMPTSKSGTNEGWMSTSTTIQTFHNNSDEWWMNKYKQSFSEVIMLNS
                     RWDDGISYGRWFICKK"
     gene            3565..4119
                     /locus_tag="PLU36_06395"
     CDS             3565..4119
                     /locus_tag="PLU36_06395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HMZ46416.1"
                     /translation="MILRKIIKYLTKTKIPANVIVGKDSVVIGPVSIHTTSSITIGND
                     CLIEGIVTTHTPQAKIEIGNEVFIGNNSFLGCADTIIIEDKVLVSFDCVIQDNDSHST
                     ISSERFTDTKDWKNGRQHNWDLTHKKPIHIKKLAWIGARAIILKGVTIGEGAIVAAGS
                     VVTKDVAPYTIVGGNPAKFIKETT"
     gene            4126..4908
                     /locus_tag="PLU36_06400"
     CDS             4126..4908
                     /locus_tag="PLU36_06400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HMZ46417.1"
                     /translation="MAYSFPERIIHKFIWFVKNFRQIKKDSVIFHAIHGPAVYNTDGL
                     ATSNNSDFLKDEKFLKAYNAAAATNPWEGFTSQWRFYIVCWFADYVKHLEGNFVECGV
                     NTGAYSKAIIEYINFDSLNKTFYLLDTFEGLVKEQITTEEEKVGVSNYLKAYTNVYEQ
                     VKETFKNNNVKIIKGIVPDTLPMVTTNKIAYLSIDMNVTKPEIEALEYFWDKMVSGGV
                     LIHDDYGFPQHIHQKHAVDEFAKRKNVKVLCLPTGQAIIFKP"
     gene            4911..5810
                     /locus_tag="PLU36_06405"
     CDS             4911..5810
                     /locus_tag="PLU36_06405"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMZ46418.1"
                     /translation="MSILIIGSEGFIGSNLVNYFKAQHDNVICADIKAIQRNDYYQIN
                     PTDPNYDELFTNHSFSICINASGSANVKFSFDQPKLDFDSNTLSVFKLLNAIKNHQVN
                     CKFLNLSSAAVYGNFSTEPLSENIHCNPVSPYGFHKWYAELICKEYHVLFNLKTISVR
                     LFSVYGEGQEKLLFWDMWQKYKANNNNVELFGSGNEARDFLHIEDVVKAIDIVYKKED
                     FNGGAINIASGKTTTIKEAATTFFELLPAKNAQYSFTHHAKKGDPDILAADISKLQQL
                     GFTPTFNLHEGLSKYMQWLQEKK"
     gene            5789..>6618
                     /locus_tag="PLU36_06410"
     CDS             5789..>6618
                     /locus_tag="PLU36_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46419.1"
                     /translation="MVTRKKIVFPYYNSYYTSTAIIIYQTNILKSLNLLDDAIKPEIY
                     IWHTHQSPLHEIKTIQYPYIHYINIKSLGFRIKKALSKMVDVLGLSKTILFNTKYDCI
                     YPSYQDNFLNGIKERIYWKADFQENYFPQYITTVEMNWVKAFFKHLQNNDGNSTLVLS
                     SYDCKNDLEKFYPEIQNPVKLFRFVSHIPKQLNFENITTIQEKFNITLPYFIVCNQYW
                     PHKNHLVVLKALHQLKQENKLNFQVVFTGKTSSIRGNEYFTELKKFITENNLENDIVI
                     T"
BASE COUNT         2324 a          987 c          992 g         2315 t
ORIGIN      
        1 aaaaactctt catttttttg aagagttttg gtaagttatt ttaaaatact tctacctatt
       61 actattcgtt gcacttcact ggtgccttca taaatctggg taatttttgc atctctcatt
      121 aagcgttcta catggtattc ttttacataa ccataaccac catgtatttg aacagcttct
      181 actgtaaccc acatagcagt ttcgcttgca aaaactttag ccatactgct acttaaagca
      241 taatccattc cattatcttt ttcccatgct gcttttaagc ataataaacg agcagcttca
      301 atacgtgtag ccatatctgc taacttaaat tgtatagctt ggtgtttggc aatttcttta
      361 ccaaaagctt tacgttcttg tgcatatttt aaagcaagct cataagcacc acttgcaata
      421 cctaatgcct gagatgcaat accaatacga cctcctgcta atgttttcat agcaaatgta
      481 aaaccaaaac catcagcacc tattctgttt tctttgggta cttttacatc tgtaaacata
      541 atagtatgtg tatcgctacc acgaatgcca agtttatttt cttttgcagc aacagtaaca
      601 ccagcccatt ttttttcaac aataaagcag ttaatacctt tgctgccttt gcttacatcg
      661 gtttgtgcca taaccaaata aacacttgca ctgctaccat tggtaatcca gtttttagta
      721 ccatttagta aataataatc tcctttatct tctgctattg ttcgttggct tgttgcatca
      781 cttcctgcct ctggttcact taataaaaat gcacctatat aaagttctcc atctttttta
      841 ccttgggcta ttggtgttaa gtatttttgt ttttgttctt cgttgccata agcttgcaag
      901 ccataacata ccaagctatt atttacactc atacatacac taacactagc atcaatttta
      961 ctcacttctt ccatggctaa tacatagctt atggtatcca ttccactacc accatattct
     1021 ggcttaacca tcatacccat aaagcctaat tcagctaatt gtgtaatttg ttctttagga
     1081 aagcgttgat gttcatctct ttcaattaca ccaggtaaac attcatttac tgcaaaatca
     1141 cgtgctgctt tttgtatcat taaatgttcc tcgctaagtt gtaaatgcat agtgttttgt
     1201 atttttagtg tggcaaaatt aggctaatag tgctcagtag aaaaataaaa aatgaatgtt
     1261 atctgttatt accattgttt atttaattac cattgcataa aataaaagta ttagctattt
     1321 tagtaaaatt tgagtattta gttttaagca tttttcatgg gaatagttag aaaacaatcc
     1381 atagcgtctt ctatatatat ttatttaggc tttgcaatag gagcttttaa tgttttattt
     1441 ctatttccta aatacttaac accagcagaa tttggattaa caagagtttt attagatgtt
     1501 tctttattaa ttgctatgtt ttgtacttta ggcagttgcc ctgcagtttt aaaattttat
     1561 cctttttatc attcttattt gccaaaaaag aaaattgaaa ttcctttctt tactatttta
     1621 ttaaccattt tggggtgtat catttttata cttatagcag atggtagcaa aagctatatt
     1681 gttagaaaat ttggcgaaaa atcgccactg tttaacgaat attattatct tatatatcca
     1741 ttaagtatct cttatgcatt ttggtattta tttgaagcat cttcctggag tattaaaaaa
     1801 acggttcttc ctaatttttt aaaagaagtt ggctttagaa ttattactac catattggta
     1861 gcattgtata ttgttaaact tatctccttt aaacagttta tttctttatt ttccttaatg
     1921 tatatagtgc cagttattat tttactttat gcattattaa aagacaattt ttttgtaatt
     1981 acttttaaca ttagctctgt tacaaaacgt ttatggaagc agattacttt gtttagcctt
     2041 tttgttttta cagggcaaac attaaatgtt attgcacgta cttcggatac aattatctta
     2101 gccagccaaa gtaaaaatgg tttggctgat gctgctgttt ttactgtggt tacttactta
     2161 gttactttaa tggatgtacc aatgagagga atgacaggca ttgcatcatc ggttattgcc
     2221 tatgcctgga aggataaaga catgaataag attaaagaaa tgtaccaaaa aactgcactt
     2281 aacttattaa ttgtaggtat aggaattttt ggaattttaa ccataaacat gttcaacttt
     2341 acacaacatt ttggtacaaa ctatagttta ttacctaccc ttttgttaat acttggcttt
     2401 gccaaattaa ttgacttagc tactggcatg aatgcacaaa tattattatc tagtaaatat
     2461 tggagaatag actttataac cagtatgggg tttgtattga tctcggtgcc attgaacata
     2521 tttttaataa aaaagtatgg tttaataggt tctgcctatg caaatttaat ttcggtgttt
     2581 atatataaca caacaaggtt actcataata tggaaattat ttaagctaca accttataac
     2641 cataaaaatt tattaaccat ggtaatagct gctgtttgct tttttattgt atatatttct
     2701 ccatctttaa acaatatata tgccgatgct attttaaaaa ctgtagtttt tatgccatta
     2761 tatatatttt tgattttgaa actaaaaatt tcagaagatt ttaaccagtt gctcctttca
     2821 tttatagaaa aaataaaaat tagaactaaa aaataatgaa gaatacaatt atatcattta
     2881 ttcctccaat tcttatagag ttaggtaaaa aaacaggaat actaaaagac aagagcaaag
     2941 taattattta caaaaaaaac gaaaatgaca cacaagattt agatatgtat tttgaccctg
     3001 agtatgctaa aatattagac tcttggggcg aaaaaacaac ttggatagaa atacaaatgt
     3061 tgcttgcaaa ttgtaaagga aaagttttag atatagcttg tggcagtggt gttactatga
     3121 gaattttgaa taagtataaa gatttagatg tgcatggttg cgatatttct gatttgttaa
     3181 ttaagaaggc tgttagcctt ggtattgcag aaaataattt aaaagttacc gatgccacta
     3241 aaatggatta tgcagattta gcttttgact attcttattc tattggttcc ttggaacatt
     3301 ttacagaaaa aggaatttct gaatttattg cagaagctgc aagggttacc aaatatacat
     3361 ctatgcacat gatgcctact tctaaaagtg gtactaatga aggctggatg agtacttcta
     3421 ctactattca aacttttcac aacaattccg acgaatggtg gatgaacaaa tacaaacaaa
     3481 gttttagtga agtaataatg ctaaactcca gatgggatga tggcatttct tatggtcgtt
     3541 ggtttatttg taaaaaatag aaatatgatt ttaagaaaga taattaaata tttaactaaa
     3601 actaaaattc cagcaaatgt tattgttgga aaagatagtg ttgtaatagg tcctgtttct
     3661 attcatacta catcttccat aaccatagga aacgattgtt taattgaagg cattgtaacc
     3721 acacatacac cacaagctaa aatagaaatt ggcaatgagg tttttatagg taataatagt
     3781 tttttaggct gtgccgatac tataattatt gaagataaag tactggtttc tttcgattgt
     3841 gttatacaag ataacgatag ccatagcact atttcaagtg aaagatttac agataccaaa
     3901 gattggaaaa atggcagaca acacaactgg gatttaacgc acaaaaaacc aatacacatc
     3961 aaaaaattag cttggattgg tgccagagcc attattttaa aaggtgtaac cattggcgag
     4021 ggagctattg ttgcagcagg aagtgttgtt accaaagatg ttgctcctta caccattgtt
     4081 ggtggtaatc ctgcaaaatt tattaaagaa acaacctaac aatgtatggc ttatagtttt
     4141 ccagaaagaa taatacataa gtttatttgg tttgtaaaaa atttcagaca aataaaaaaa
     4201 gactctgtaa tttttcatgc tatacatggc cctgcagtgt ataatactga tggattggca
     4261 acaagcaaca actctgattt tttaaaagac gaaaaatttt taaaggcata taatgcagct
     4321 gcagcaacta atccctggga aggttttact tctcaatggc gtttttacat tgtttgttgg
     4381 tttgcagatt atgtaaaaca tttagaagga aattttgtag aatgtggtgt aaacacaggt
     4441 gcttacagta aagcaattat agaatatata aactttgaca gtttaaataa aaccttttat
     4501 ttattagata cttttgaagg attggtaaaa gaacaaataa caacagaaga agaaaaagtt
     4561 ggagtaagca attacttaaa agcttatacc aatgtttatg aacaagtaaa agaaactttt
     4621 aaaaataata acgtaaaaat tattaaaggt atagtgccag atacactgcc tatggttaca
     4681 actaataaaa ttgcttattt atctattgat atgaatgtta ccaaaccaga aatagaggct
     4741 ttggaatatt tttgggataa aatggtaagt ggaggtgttt taatacatga tgattatggc
     4801 tttccgcaac atattcatca aaaacatgca gtagatgaat ttgctaaaag aaaaaatgta
     4861 aaagtattgt gtttgcctac tggccaggca attattttta aaccttaatt atgtcaatac
     4921 taattattgg ttctgaaggt tttataggaa gtaatttagt taattatttc aaagcacagc
     4981 atgataatgt tatttgtgcc gatattaaag caattcaaag aaatgattat tatcaaataa
     5041 atccaacaga ccctaattat gatgaattat ttaccaatca tagctttagt atttgcataa
     5101 atgcaagtgg tagtgctaat gtaaaatttt cttttgacca gcctaaatta gattttgaca
     5161 gcaacacttt atctgttttc aaattattaa atgcaataaa aaaccatcaa gtcaactgta
     5221 aatttttaaa cctttccagt gctgcagtat atggtaattt ttcaacagaa cctttatcag
     5281 aaaatattca ttgtaatcca gtatcgcctt atggatttca taaatggtat gcagagttaa
     5341 tttgcaaaga ataccatgtt ttatttaatt taaaaactat aagtgtacgc ttattttctg
     5401 tatatggaga aggacaagaa aaattattgt tttgggatat gtggcaaaaa tataaagcca
     5461 ataataataa tgtagaacta tttggcagtg gtaatgaagc aagagatttt ttgcatattg
     5521 aagatgttgt aaaagctata gatatagttt ataagaaaga agattttaat ggtggagcta
     5581 tcaatattgc aagtggcaaa acaacaacaa ttaaagaagc agcaactaca ttttttgaat
     5641 tattaccagc aaaaaatgca caatactcct ttacgcatca tgccaaaaaa ggcgacccag
     5701 atattttagc tgctgatata agcaaactac aacaattagg ctttacacct acatttaatt
     5761 tacacgaagg actttctaaa tacatgcaat ggttacaaga aaaaaaatag tttttcctta
     5821 ttacaatagt tattacacca gtactgctat tataatttat caaaccaata tattaaaatc
     5881 attaaacctt ttagatgatg ccataaagcc agaaatttat atttggcata cacatcaatc
     5941 tccattgcat gaaattaaaa ccatacaata tccatacatt cattacatca atataaaatc
     6001 gttgggcttt agaattaaaa aagctttatc taaaatggta gatgtgcttg gattaagtaa
     6061 aactatttta ttcaacacaa aatacgattg catttatcca tcttatcagg ataatttttt
     6121 aaacggaata aaagaaagaa tttattggaa agccgatttt caggaaaact attttcctca
     6181 atacattacc acagtagaaa tgaattgggt aaaagcattt tttaaacatt tacaaaataa
     6241 cgatggtaac agtactttag ttttaagtag ttacgattgt aaaaatgatt tagaaaaatt
     6301 ttatcctgaa atacaaaatc ctgtaaaatt attcaggttt gtatcgcata ttcctaagca
     6361 gttaaacttt gaaaatataa ctaccataca agaaaaattc aacattacac tcccctattt
     6421 tattgtgtgc aatcaatact ggccacataa aaatcattta gttgttttaa aagcattgca
     6481 tcaattaaaa caagaaaaca aactaaactt tcaagtagtt tttacaggaa aaacaagcag
     6541 tataagaggc aatgaatatt ttactgaatt aaaaaaattt attactgaaa ataatttaga
     6601 aaatgatatt gttattac
//
LOCUS       DAOQPK010000111         6593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_6678_length_6593_cov_4.857755, whole genome shotgun sequence.
ACCESSION   DAOQPK010000111 DAOQPK010000000
VERSION     DAOQPK010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6593
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6678_length_6593_cov_4.857755"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..1755
                     /gene="ade"
                     /locus_tag="PLU36_06415"
     CDS             88..1755
                     /gene="ade"
                     /locus_tag="PLU36_06415"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HMZ46420.1"
                     /translation="MEQKSFSIKGNIVDVVHKQIYAGEVKVVNGKIASVTPLPKVETD
                     QFILPGFIDSHVHVESSMLVPSEFARLAVVHGTVATISDPHEIANVCGIQGVEFMIEN
                     GKKVNFKFNFGAPSCVPATSFETAGAVLNSNDVKTLLAKEEIIYLSEMMNFPGVLFKD
                     EEVMKKLQAAIDAGKPIDGHAPGLRGEKAKQYIEAFGKNGEIIISTDHECFTKEEALD
                     KLKYGMKIIIREGSAAKNFEALIDLLHDYEDKIMFCSDDKHPDSLAIGHINQLCARAV
                     AKGIDIFKVLKAACLNPIIHYKMNVGLLQAGNDADFIIAEDLKSFKIIQTYIKGKLVA
                     ENGKTFIPSVPVTAINQFNCNEITKTQLQIKLGDYPTVDNKIYVIEALEGQLITNKLL
                     LQGKQENKEWVSNIENDILKMVVVNRYTNAPIAKCFIKNFGLKQGAMASSVAHDSHNI
                     VAVGCNDESLAKAINLVIKEQGGISAVSNNNEKVVGLPIAGLMSAEDGYKVANAYTDI
                     DLMTKKELGSTLAAPFMTLSFMALLVIPYLKLSDKGLFDGEKFEFVN"
     gene            1763..2350
                     /locus_tag="PLU36_06420"
     CDS             1763..2350
                     /locus_tag="PLU36_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HMZ46421.1"
                     /translation="MIIIDNKLISDEIVEEQFVCDLEKCKGGCCEDGDAGAPLEDWEK
                     KEVEKFYSSVLPYLTQDGIEEINKQGLFVYDKTFGWVTPTINGGICVYAKKDHQNIIH
                     CAFEQAYNDGKISWKKPLSCHLFPIKTSISKEDPDLEYINYEPREDLCKAACSLGKKL
                     KIPAYIFLKEALIRKYGEEFYNALEASANHLKSNK"
     gene            2570..4447
                     /locus_tag="PLU36_06425"
     CDS             2570..4447
                     /locus_tag="PLU36_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HMZ46422.1"
                     /translation="MLIKGIPISLSLNNSINDTEADEKNKYQKKRLIKLSLLAIAVAM
                     VVSGFAKVLVHLIDLITNIAFYQNFSFIPSSPANNSIGWWVVLIPVVGGLLVGIMALY
                     GSKAIRGHGIPEAMEQILTNQSKIKPSITFLKPISAAISIGTGGPFGAEGPIIATGGA
                     LGSTLGQILKITPNERKILLASGATAGMSAIFGSPIAAIFLAIELLLFEFSPRSFIPV
                     ALACITGAAGHHFLFSSSVVFPMLQTILPASNKALLFYSIMGVAVGILSVIATKIVYW
                     IEDAFEKLPIHWALWPAIGGIAVGLIGYYEPKTLGVGYNNITDVLSGTLTFNVILSLC
                     LLKFTSWAIAIGSGTSGGTLAPLLTIGGAIGALLGILGNQWFPAIGISIPLCALVGMS
                     AMFAGASRAFLTSIVFALETTGQSNALLPLLATCTGAYCVSYLLMTNTIMTEKIARRG
                     VQTPTSYEPDILDKTLVEELPLDDVIILNDAMNVGEVRTWLKQEADAITSYFIVSDLD
                     GTYKGLISYSSLFNMQHNNDLIIGDLVKRKNIFIQPSANLKTAVETMSKEDVDILPVI
                     EEDHIIGILTYQNILSIYKRSVFEKENKQVQISLSRQRLKMLVRGQKIISILKSKKAN
                     T"
     gene            complement(4458..6566)
                     /locus_tag="PLU36_06430"
     CDS             complement(4458..6566)
                     /locus_tag="PLU36_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HMZ46423.1"
                     /translation="MAKADEGMWLPQLLQQLNEGRMKAMGMKISAKDIYDINKSSLKD
                     AIVSFGGFCTGEIISSKGLILTNHHCGFRAIQAHSTIENNIIENGFWAKTHKDELGNK
                     GLYVTFIKNIIDVTNKVLAGVNESMQPNERQAVIDKNIDNINSSTKLGKFEKSFVREF
                     FDGNMYYLFVTETFNDVRLVGAPPNSIGNFGVDTDNWVWPRHTGDFSLFRIYANKNNE
                     PADYSSDNIPYTPKRFLNISMKGIKENDFTMVFGFPGKTNEYLPSEAVRQIAQINDPA
                     KIYVRDKALAVLSKYMRNDMSIKIQYAAKYAGIANAWKKWQGEVLGLTKTNAVEKKLQ
                     YEQVYNTKLKANTTLYNKYNGVLDKLINAYKRIEQYGLARDYYNETISKIELFSLANR
                     IIRLKNVQKNGAEKFKNAVDEQIKSLPDFYKDFNAMVDKDMFIAMMDIYLNKMPKEFI
                     SNTVMEMYKANGADLNKWADKIYSNCTLVNKEKTLDALNNNAALLIDNTLEDDLVKLM
                     LAIRRHYSEAVAGELVKIQDEIDQLQRQYMKAQMEVMKDKKFYPNANSTLRVTYGKVD
                     GYSPADARVYKFQTYLDGVMEKYKPGDYEFDVNEKLRTLYKNKDYGIYGVKGKMPVCF
                     IASNHTTGGNSGSPALNAYGNLIGLNFDRGWEGTMSDINYDASICRNIMVDIRYILFI
                     IDKYAGAGHLVKEMKLMYDK"
BASE COUNT         2222 a          979 c         1058 g         2334 t
ORIGIN      
        1 tttttcatta tacttttata ttttattgct caaatttagt ttgcttttgg aattaattta
       61 ataagaataa aattttaata aaatattatg gaacaaaaaa gcttttcaat aaaaggaaat
      121 attgtagatg tagtacataa acaaatttat gctggtgagg taaaagttgt aaatggaaaa
      181 atagcatctg taacaccttt gcccaaagta gaaacagatc aatttatttt acctggtttt
      241 atagatagcc atgtacatgt tgaaagtagc atgcttgtac ccagcgaatt tgcaagatta
      301 gctgttgtac atggcaccgt tgcaaccatt agcgaccctc atgaaattgc caatgtttgt
      361 ggcattcaag gagttgagtt tatgattgaa aacggaaaga aagtaaattt taaatttaat
      421 tttggagccc caagttgcgt acctgcaacc agttttgaaa ctgctggtgc tgttttaaac
      481 agcaacgatg taaaaacatt gttggcaaaa gaagaaatta tttatttaag tgaaatgatg
      541 aattttcctg gcgttttatt taaagatgaa gaagtaatga aaaaattaca agcagctata
      601 gatgcaggaa agccaataga tggtcatgca ccaggtttga gaggagaaaa agcaaaacaa
      661 tatattgaag catttggaaa aaatggtgaa ataataattt ctaccgacca tgaatgtttt
      721 actaaagaag aagctttaga caaattaaaa tatggaatga aaattattat tagagaagga
      781 agtgctgcaa aaaactttga agcattaatt gatttattac acgattacga agataaaata
      841 atgttttgca gcgatgataa gcaccctgat agtttagcca ttggtcatat taaccaatta
      901 tgtgcaagag ctgtagcaaa aggtattgat atttttaaag ttttaaaagc ggcttgttta
      961 aacccaatta tacattataa aatgaatgtt ggcttattac aagcaggcaa tgatgcagat
     1021 tttattatag ccgaagattt aaaaagtttt aaaattattc aaacatatat taaaggcaag
     1081 ttggttgcag aaaatggaaa aacttttatt ccatctgtac ccgttactgc aattaatcag
     1141 tttaactgta atgaaattac aaaaacacaa ttgcaaataa agctgggaga ttacccaact
     1201 gttgataata agatttatgt tatagaagcc ttagaagggc agctaataac caataaactt
     1261 ttattgcaag gcaaacaaga aaataaggaa tgggtaagca atattgaaaa cgatatttta
     1321 aaaatggtgg tggtaaatag atatactaat gcaccaattg ctaaatgttt tattaagaac
     1381 tttggtttaa agcaaggagc aatggcatct tcggttgcac acgatagcca taatattgtg
     1441 gctgttggtt gtaacgatga aagccttgct aaagcaatta atttagttat aaaagaacaa
     1501 ggaggtatta gtgctgtaag caacaataac gaaaaagtgg ttggcttgcc aattgcaggt
     1561 ttaatgagtg cagaagatgg ttataaagtt gccaatgctt atacagatat agatttgatg
     1621 actaaaaaag aattaggtag tacattagca gctccattta tgactttgag ttttatggct
     1681 ctattggtta ttccttattt aaaactgagt gataaaggat tatttgatgg agaaaaattt
     1741 gaatttgtga attaattatt ttatgataat tatagataat aaactaatta gcgatgaaat
     1801 tgttgaagaa caatttgttt gtgatttaga aaaatgtaaa ggtggttgct gcgaagatgg
     1861 agatgcaggt gcaccattag aagattggga aaagaaagag gttgaaaaat tttactcctc
     1921 tgttttacca tacttaacac aagatggaat tgaagaaata aataaacaag gtttatttgt
     1981 ttatgataaa acttttggct gggtaacacc aaccattaat ggaggtattt gtgtatatgc
     2041 aaaaaaagat catcaaaata ttattcattg tgcatttgag caagcctata acgatggaaa
     2101 aataagttgg aaaaagcctt taagttgcca tttatttccc attaaaacat ctataagcaa
     2161 agaagaccct gatttagaat atattaatta cgagccaaga gaagatttat gtaaagcagc
     2221 ttgcagcctt ggtaaaaaat taaaaatacc agcatatatt tttttaaaag aagccttaat
     2281 aagaaaatat ggagaagagt tttataatgc attagaagca agtgctaatc atttaaagag
     2341 taataagtaa cctactctgt ttaattcatc ttaactaaaa ataagtatta aataagtttg
     2401 catttttatt ataaaaaaca atttactctt taatgtatta gttagaagtt attttttatc
     2461 atcaacataa ataacctctt tatggcatta taattgcata aatttgcaaa cctaatcaac
     2521 actctcaaac cctttcttaa caatttattg tttacttaaa ccaacatgaa tgttaattaa
     2581 aggaattcct atttctctat ctcttaataa ctctatcaac gatacagaag cagatgaaaa
     2641 aaataaatac cagaaaaaaa gattaataaa actttcttta ctagctatag ctgttgcaat
     2701 ggttgttagt ggttttgcaa aagttttagt acacttaata gatttaataa ccaatattgc
     2761 tttctatcaa aattttagtt tcataccatc aagtcctgca aataactcta ttggttggtg
     2821 ggttgtgtta attcctgtag ttggaggttt attggtaggc ataatggcct tatatggttc
     2881 caaagctata cgtggccatg gtattccaga agcaatggaa caaatactaa ctaaccaaag
     2941 taaaataaaa ccttctatta cttttttaaa acccatttct gctgcaatat ctattggcac
     3001 tggtggacca tttggtgctg agggcccaat aattgcaaca ggtggtgcat taggctctac
     3061 acttggtcaa attttaaaaa ttacaccaaa cgaaagaaaa attttactag catctggtgc
     3121 aacagcaggt atgtcggcaa tttttggtag cccaatagca gcaatttttt tagcaataga
     3181 attgttgttg ttcgagtttt ctcctcgttc ttttattccg gttgcattag cttgtattac
     3241 tggtgctgca gggcatcatt tcttgtttag cagtagtgta gtttttccaa tgttgcaaac
     3301 tattttacct gccagcaata aggcattatt attttatagt attatgggtg ttgctgttgg
     3361 tatattatct gtaatagcaa ccaaaatagt gtattggatt gaagatgctt ttgaaaaatt
     3421 acctatacat tgggctttgt ggcctgcaat tggtggtatt gctgttggct taataggtta
     3481 ttacgaacct aaaactttgg gtgttggtta taataatatt acagatgttt tatctggcac
     3541 tttaactttt aatgttattc tatcattatg tttattaaag tttacatctt gggctatagc
     3601 tattggtagt ggaacatctg gtggtacttt ggccccatta ttaactattg gtggtgccat
     3661 tggtgcattg cttggcattt taggtaatca atggtttcct gcaattggca tttctatacc
     3721 attatgtgca ttggttggta tgtctgcaat gtttgcaggt gcatcaagag catttttaac
     3781 ctcaattgtt tttgctttag aaactactgg tcaatccaat gcattattac ccttgctggc
     3841 aacttgtaca ggtgcttatt gtgtttctta tttattaatg actaatacca ttatgacaga
     3901 aaaaattgca agaagaggtg tacaaacacc tacctcttat gaacctgata ttttagataa
     3961 aaccttagta gaagaactgc cattagatga tgtaattatt ttaaatgatg caatgaatgt
     4021 tggtgaagtg agaacttggc taaaacaaga agcagacgct attacaagtt attttattgt
     4081 aagtgattta gatggaactt ataaagggtt aattagctac tcaagcttat ttaacatgca
     4141 acataataat gatttaatta ttggtgattt agtaaaacga aaaaatattt ttatacaacc
     4201 atctgctaat ttaaaaacag cagttgaaac catgtctaaa gaagatgtag atatattacc
     4261 tgttattgaa gaagaccata ttattggtat tttaacttac caaaatatat tatctatata
     4321 caaacgttct gtatttgaaa aagagaacaa acaagtacag atttctttaa gtaggcaacg
     4381 tttaaaaatg ttggttcgtg gccaaaaaat tatttctatt ttaaaatcta aaaaagctaa
     4441 tacttaatag tccaaaatta tttatcgtac attaatttca tttctttaac taaatgtcct
     4501 gcaccagcat atttatctat aataaataaa atgtaacgaa tatctaccat aatatttcta
     4561 caaatactgg catcataatt aatatcactc atggtgcctt cccaccctct atcaaagttt
     4621 aaacctatta aattgccata agcatttaaa gcagggcttc cactattgcc tcctgttgta
     4681 tgatttgatg cgataaagca aacgggcatt tttcctttta ctccataaat tccatagtct
     4741 ttatttttgt atagggttct taatttttca tttacatcaa attcataatc tcctggttta
     4801 tatttttcca tcacaccatc taaatatgtt tgaaatttgt acactctagc atcggctggc
     4861 gaataaccat ctactttacc ataagtaact cttaatgtac tattagcatt ggggtaaaat
     4921 ttcttatctt tcattacctc catctgtgct ttcatatact gcctttgaag ctgatcaatt
     4981 tcatcttgta ttttaaccaa ttcgcctgca acagcttctg aataatgcct tctaattgcc
     5041 agcatcagct taactaaatc atcttctaaa gtattatcta ttaaaagagc agcattatta
     5101 tttaaagcat ctaaggtttt ttctttatta accaaagtac aattgctata tattttatca
     5161 gcccacttgt ttaagtctgc accattggct ttatacattt ccattactgt attagatata
     5221 aattctttgg gcattttgtt taaataaata tccatcattg caataaacat atctttatca
     5281 accattgcat taaaatcttt ataaaaatcg ggtaagcttt ttatttgttc atcaaccgca
     5341 tttttaaact tttctgctcc atttttttgt acatttttta atctaattat tctatttgct
     5401 aaactaaaca gttcaatttt acttatagtt tcgttataat aatctcttgc aagtccatat
     5461 tgctctatgc gtttatatgc atttattaat ttatctaata caccattata cttattataa
     5521 agcgttgtat tggctttaag ttttgtatta tatacttgtt cgtactgtag ttttttttct
     5581 actgcatttg ttttggttaa gcctaaaact tctccttgcc attttttcca ggcattagca
     5641 ataccagcat atttagctgc atattgtatt tttatagaca tatcgttacg catatattta
     5701 cttaatacag ctaatgcttt atctctaaca taaattttag cagggtcgtt tatttgtgca
     5761 atttgtctaa cagcttcgct tggtaagtac tcgtttgttt taccaggaaa accaaatacc
     5821 attgtaaaat cattttcttt aatacccttc atagatatat ttaagaacct ttttggggta
     5881 taaggaatat tatcagaaga gtaatctgca ggctcattat ttttatttgc ataaattctg
     5941 aataaggaaa aatctccggt atgccttggc caaacccaat tatctgtatc tacaccaaaa
     6001 ttgccaatag agtttggtgg tgcacccact aatcttacat cgttaaaagt ttctgttaca
     6061 aataagtaat acatattgcc atcaaaaaat tctcgaacaa aagacttttc gaacttgcct
     6121 aatttggtgg atgaatttat attgtcaatg tttttatcaa ttacagcttg tctttcgttt
     6181 ggttgcatgc tttcatttac acctgctaaa actttgttgg ttacatcaat aatatttttt
     6241 ataaaagtaa catacaagcc tttattgcct agttcatctt tatgggtttt agcccaaaag
     6301 ccattttcta taatattatt ttctatggtt gagtgtgctt gtattgctct aaagccacaa
     6361 tgatgattag ttaatattaa tcctttgcta gaaattattt cgccagtgca aaaaccacca
     6421 aagctaacaa tagcgtcttt taagctactc ttgttgatgt cgtaaatatc ttttgccgaa
     6481 attttcatac ccattgcttt cattctacct tcattaagtt gttgcaataa ttgaggcaac
     6541 cacattcctt catctgcttt ggcaattaga acagaactag ttaaaaaaga gaa
//
LOCUS       DAOQPK010000112         6591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_6681_length_6591_cov_4.699663, whole genome shotgun sequence.
ACCESSION   DAOQPK010000112 DAOQPK010000000
VERSION     DAOQPK010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6681_length_6591_cov_4.699663"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2420)
                     /gene="mutS"
                     /locus_tag="PLU36_06435"
     CDS             complement(<1..2420)
                     /gene="mutS"
                     /locus_tag="PLU36_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HMZ46424.1"
                     /translation="MAKAASVSPMMQQHKAIKQKYPDAILLFRVGDFYETFGQDAINA
                     SQVLGITLTKRNNGDVGNTELAGFPHHALDTYLHKLVKAGYRVAICDQLEDPKQAKGV
                     VKRGVTELVTPGVATNDKLLENSNNNFLAAIHFAESNVGIAFLDISTGEFFVAEGNEE
                     YIDKLLQSLKPAEVIFQRSSQKQFKETFGVKFYTYTLESWIFELGFATESLIKHFQTH
                     SLKGFGIENMQHSIIAAGAILYYLKDTEHSNLQHITTIQRIEREEFLWMDKFTIKNLE
                     IIGSPGAEGNNLLKVLDNTVSPMGARLLKRWVVMPLKNLDKINERLQVVEDFIVDTDN
                     RLQLINAIKHCGDIERLVSKIPLKKINPREVIQLAKGLQQVAIIKKICSSFKNPYLQR
                     LSDNLNNCTVILEKILTEIQEEPPVTTNKGGIIKTGVHHELDELRKIASGGKEYLINI
                     QQREADTTGIPSLKIGFNNVFGYFLEVTNTHKNKVPASWIRKQTLANAERYITTELKE
                     YEEKIIGAEEKIITIELEIFEKLLLALQDYIAPIQVNGNIVAIVDCLLCFSENALKYK
                     YKKPQLHNGLELNLKESRHPVLERNLPEGKAFIANDIYADADSQQIIILTGPNMSGKS
                     AILRQTALITLMAHIGSFVPVDTAKIPLTDKIFTRVGASDNLSGGESTFMVEMNETAS
                     IINNISNRSLILLDEIGRGTSTYDGISIAWSIVEYLHQSTAKPKTLFATHYHELNELE
                     EKLTRVKNYHITNKEVGNKIIFLRKLAKGGSTHSFGIHVAKMAGMPQELINRANEILK
                     ILEQKTEIP"
     gene            2446..3183
                     /locus_tag="PLU36_06440"
     CDS             2446..3183
                     /locus_tag="PLU36_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ46425.1"
                     /translation="MASIKIKQNTNFYIHFLIRYLVLSVPIKHSMQIRLNKIIPIPLK
                     DKLLQRKSDVWNTDLIFNIQQLVKINAPSGTGKTTLIHILYKLRNDYSGIVKWNDELL
                     ENINDDKLANYRKHKLSIVFQDLKLFQNLTAKENIELNRVLQKPYYEEKVIVEMAEEL
                     GVDSILQQPIRLCSYGEQQRIAIIRALIQPFELLLMDEPFSHLDYDNTVKAAALIAKE
                     CTKRNAGFVLTDLDDDNHFLYSQKLRL"
     gene            3189..4376
                     /locus_tag="PLU36_06445"
     CDS             3189..4376
                     /locus_tag="PLU36_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46426.1"
                     /translation="MLFTLLKKIIKTATGRTKFIMAFIGLSVALLLILSAIQIQANYN
                     QLLYGKNNTDSTANFLVVNKEVNDNTVNATILSNEVLHEIKQQPFIDAVGYISGGRYK
                     ASIESLIQSFPFYTDIAFESVQNDFIDVNSKDWAWNENSNFIPMIIPNMFLDIYNFQF
                     SISQGYPQITPETLKMMRFRVNLYYANGNSENYIGRIVGFSDRISSVLVPQSFMDWAN
                     KKYGNSKTTNPGRVVIKTKDPGNPELVKFLKSKGLTTDADKTRFSKYRQIVNAVVNVS
                     WVTGAIMLLFALLVFTMFIQLTVASCKEEIKLLITIGTSPIQLKSFLMKQFFPSNIVI
                     VGLALMLVSIVQFMLYKYLQAQNIFLSAYISIYTIVAACIVLMLLWLVNHFTIKKYIE
                     VKV"
     gene            complement(4378..5268)
                     /locus_tag="PLU36_06450"
     CDS             complement(4378..5268)
                     /locus_tag="PLU36_06450"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HMZ46427.1"
                     /translation="MFINLSEINLIERQAWLQHAIAPRPICFVSTTDKEGNINLSPFS
                     FFNLFSSNPPIVIFSPARSGRTGLLKDTFLNVQEVPEIVINICDFDMVQQVSLSSCEY
                     PKQVDEFIKAGFTKVQSTMVKPPMVKESKINLECKVIETKSLGEGGGAGQLVFAEVLC
                     MHINESILNEDKSMIDHQKMHHIARLGGDYYASITPENLFTVPKPIRELGIGVDALPE
                     GIRNSTILTGNNLGMLANVQSLPQVDATFTDETLKNIVQYFSASPNEMEKELHNYAKE
                     LLDNKKVKEAWQVLLTLENN"
     gene            complement(5552..5947)
                     /locus_tag="PLU36_06455"
     CDS             complement(5552..5947)
                     /locus_tag="PLU36_06455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HMZ46428.1"
                     /translation="MLQKNTLQFLSSLKNNNNKIWFDANRGWYDETKADFINLNEAII
                     KKLKKKDTTLEPLIAKNCIFRINRDVRFSPNKTPYKTNMGASINKGGKKIANAGYYLH
                     IEPGASFLAGGIWMPKTIELKKNKTRNRL"
     gene            complement(6098..>6591)
                     /locus_tag="PLU36_06460"
     CDS             complement(6098..>6591)
                     /locus_tag="PLU36_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46429.1"
                     /translation="YIMDILHPEFIVFLSCAAKNNLRYLLIGGYAVNYYGYNRHTHDL
                     DVWLAPTNENKLAFIQTLLCMQYSENEVALLYEEDFTKPFKANIGTPTADIDVLTFVH
                     SKISFDDAEKNKVIFAIEKDVTMNIVPYDFLRDMKLFAARNKDLLDVSELEKLRKNKK
                     KNK"
BASE COUNT         2133 a         1050 c          950 g         2458 t
ORIGIN      
        1 ggtatttctg ttttttgttc taatattttt aaaatttcat ttgctctgtt tattaactcc
       61 tgtggcattc ctgccatttt tgccacatga ataccaaaac tatgtgtact gccacctttg
      121 gctaattttc gtaaaaaaat aattttattt ccaacttctt tattggtaat atgataattt
      181 ttaacacggg ttaatttttc ttccaattca tttaattcgt gataatgtgt tgcaaataat
      241 gttttaggtt ttgcggtaga ttggtgtaaa tattctacaa tactccaagc aatgctaata
      301 ccatcgtaag tagaggttcc cctgccaatt tcgtctaata aaattaaact tctgttagaa
      361 atattattga tgatggaggc ggtttcattc atttctacca taaaagtact ttctccgcca
      421 cttaaattat cacttgctcc tacacgggta aaaattttat cggttaaagg aatttttgca
      481 gtatctactg gtacaaagct accaatgtgg gccattagtg ttattagtgc tgtttgcctt
      541 aatatggcac ttttaccact catatttggg ccagtaagaa tgattatttg ttggctatct
      601 gcatctgcat aaatatcatt tgcaataaat gctttgcctt ctggtaaatt tctttcaaga
      661 actgggtgcc tactttcttt caaatttaat tcaagcccat tatgcagttg aggctttttg
      721 tatttgtact ttaaggcatt ttcagaaaag caaagtaaac aatctacaat agctactatg
      781 ttaccattta cttgtatggg tgctatataa tcttgtaatg caagtaataa tttttcaaaa
      841 atttcaagtt caattgtaat aattttttct tcagcaccta ttattttttc ttcatattct
      901 ttaagttctg ttgtaatata tctttctgca tttgctaaag tttgttttct tatccagctt
      961 gcaggcactt tatttttatg tgtattggta acttctaaaa aatatccaaa aacattatta
     1021 aatccaattt ttaaagaagg aatacctgtt gtatctgcct ctctttgttg aatattaatt
     1081 aaatattctt taccacccga agctattttt cttagctcat ctaactcatg atgaacacca
     1141 gttttaatta ttccaccttt attagttgta acaggaggct cttcctgaat ttcggttaat
     1201 attttttcca gaattaccgt acaattgttt aaattatctg aaagcctttg taagtaaggg
     1261 tttttgaagg aggaacaaat ttttttaata atggcaactt gttgtaatcc tttagcaagt
     1321 tgtataacct ctcttggatt tatttttttt aaaggaattt tactaaccaa cctttcaata
     1381 tcgccacaat gctttattgc gttaattagt tgaagccgat tatctgtatc tactataaaa
     1441 tcttctacaa cttgtaaccg ttcatttatt ttatccaaat tttttaaagg cattactacc
     1501 cagcgtttaa gtagccttgc acccattggc gaaacagtat tgtctaaaac ttttaataag
     1561 ttattacctt ctgcaccagg gctgccaata atttcaagat ttttgattgt aaatttatcc
     1621 atccacaaaa attcttctct ctctattctt tgtattgttg ttatatgttg aaggtttgaa
     1681 tgttctgtat cttttaaata atacaaaatg gcaccagcag caataataga gtgctgcata
     1741 ttttcaattc caaacccttt taaagaatgt gtttgaaaat gttttattaa actttctgtt
     1801 gcaaagccaa gttcaaaaat ccaactttct aaagtgtagg tataaaattt tacgccaaat
     1861 gtttctttaa attgtttttg tgagcttctt tgaaaaataa cttctgcagg ctttaagctt
     1921 tgtaaaagtt tatctatata ttcttcattt ccttctgcta caaaaaactc tccagtacta
     1981 atatctaaaa aggcaatacc tacattactt tcagcaaaat ggatagctgc taaaaaatta
     2041 ttattactat tttccagaag tttatcgtta gtagcaacgc caggtgtaac caactctgta
     2101 acaccacgtt ttacaacacc tttggcttgt ttagggtctt ctaactgatc gcaaatagca
     2161 acacgataac cagcttttac taatttatgt aggtaagtat ctagtgcatg atgaggaaag
     2221 cctgccaatt ctgtattacc tacatcacca ttatttcttt tagtaagtgt aatgcccagc
     2281 acctgcgatg cattgattgc atcttgccca aaagtttcgt aaaaatcacc aactctaaac
     2341 aacaaaatag catcggggta tttttgttta attgctttat gttgttgcat cataggcgat
     2401 acagaagcag cttttgccat agttagcaaa tataaacatt gtacattggc atcaataaaa
     2461 attaaacaaa atactaattt ttatatccat ttcttaatac gatatttagt actttcggta
     2521 cctattaaac atagtatgca aataaggctt aataagataa tacctatacc acttaaagat
     2581 aaattactac agagaaaatc tgatgtttgg aatactgatt taatttttaa tatccaacag
     2641 ttggtaaaaa taaatgcacc cagtggcaca ggtaaaacta cactaataca tatattgtat
     2701 aaacttagaa atgattattc tggtattgta aaatggaatg atgaattgct ggaaaatatt
     2761 aatgacgata aattggctaa ttatcgtaaa cataaattaa gcattgtttt tcaagattta
     2821 aaactttttc aaaatcttac tgcaaaagaa aatattgagc ttaacagggt tttacaaaaa
     2881 ccatattatg aagaaaaggt aattgtagaa atggctgaag aattaggtgt tgattctatt
     2941 ttacagcaac ccataaggct ttgtagctat ggagagcaac aacgaattgc tataataaga
     3001 gctttaattc aaccctttga gttgttgtta atggatgagc catttagcca tttagattat
     3061 gataatactg ttaaagcagc tgcattaatt gcaaaagaat gcactaaaag aaatgcagga
     3121 tttgtattaa cagaccttga tgatgacaat cattttttat attcacaaaa attaaggctt
     3181 taattaaaat gctttttaca cttttaaaaa aaataattaa aacagctacc ggcagaacaa
     3241 agtttataat ggcttttatt ggcctgtcgg ttgcattgtt acttatttta tctgccatac
     3301 aaatacaggc aaactataat caattattgt atggcaaaaa taatacagat agtacagcca
     3361 attttttagt agtaaacaaa gaggtaaatg ataacacagt aaatgcaaca attttaagta
     3421 atgaggtttt acatgaaata aaacagcagc cttttattga tgcagttgga tatataagtg
     3481 gtggaagata taaagcatct atagaaagtt taattcaatc ttttcctttt tatactgata
     3541 ttgcatttga aagtgtacaa aatgatttta ttgatgtgaa tagtaaagat tgggcttgga
     3601 atgagaactc taattttatt cctatgatta ttcccaacat gtttttagat atttataatt
     3661 ttcaattctc catttctcaa ggctatcctc aaattactcc agaaacctta aagatgatgc
     3721 gttttagggt taatctttat tatgcaaacg gaaactcaga aaattacatt ggcagaattg
     3781 ttggttttag tgatagaatt tcgagtgtat tggtaccaca gagttttatg gattgggcca
     3841 acaaaaaata tggcaattcc aaaactacta acccaggtag agttgtgata aaaaccaaag
     3901 atcctggcaa tccagaatta gtaaaatttc taaaaagcaa aggtttaaca accgatgcag
     3961 ataaaaccag atttagtaag tataggcaga ttgtaaatgc agttgttaat gtaagttggg
     4021 taactggtgc tattatgctt ttgtttgcat tactcgtatt tacaatgttt atccagctta
     4081 ctgttgcctc gtgtaaagaa gaaataaaat tattaattac tattggcaca tcgccaatac
     4141 aattgaaaag ctttttaatg aaacaatttt ttccttctaa tattgtaatt gttggtttag
     4201 cattaatgct tgtttctatt gtacaattta tgctatataa atatttacaa gcacaaaaca
     4261 ttttcttgtc tgcttatatt tctatttata ctattgttgc agcctgtatt gttttaatgt
     4321 tgttatggct ggtaaaccat tttaccatta aaaaatatat tgaagtgaaa gtgtaggtta
     4381 attgttttct agagttaata atacttgcca agcctccttt acttttttat tatctaataa
     4441 ctctttggca taattgtgta attctttttc catttcgtta gggcttgcag aaaaatattg
     4501 aacaatgttt tttaaagttt catcagtaaa tgttgcatca acctgtggta aactttgcac
     4561 attagccaac atgcctaaat tgttacctgt aagtattgta ctatttctta taccttctgg
     4621 caaagcatct acaccaatgc ccaactctct aattggtttt ggaacagtga ataaattttc
     4681 tggtgtaatg cttgcataat agtctcctcc aagccttgca atatgatgca ttttttgatg
     4741 gtcaatcatg cttttatctt catttaaaat gctttcgtta atgtgcatac acaatacttc
     4801 tgcaaaaacc aactgaccag caccaccacc ttctcctaaa ctttttgtct caattacttt
     4861 acattctaaa tttattttag actctttaac cattggaggt ttaaccatag tagattgtac
     4921 ttttgtgaaa ccagctttaa taaactcatc tacctgtttg ggatactcac aactgctaag
     4981 gcttacttgt tgtaccatat caaaatcaca aatattaatt acaatttctg gcacttcttg
     5041 tacgtttaaa aaagtatctt tcagtaatcc agttctaccg ctacgggcag gagaaaatat
     5101 tacaataggc ggattggaag aaaataaatt gaagaaagaa aaaggactaa ggtttatatt
     5161 cccttcttta tctgttgtgc ttacaaaaca aatagggcgt ggtgcaattg catgttgtaa
     5221 ccatgcttgt cgttctatta aattaatttc agataaattg ataaacataa aatgttttta
     5281 atcttctatt gctttattga taaaatatat taatggtgtt atagtttcaa aattactaac
     5341 aatattttga atacaatttt ctttaagtag ctcttcgtct aaaatatttt tagatacaat
     5401 ataactttta aactttaaat attcaatagc gggattgtct atatcatatc cttttggtgg
     5461 acgagttaaa acatattcct tgcttttatc aatcccacca aattgttgaa taattttttg
     5521 ggatgcaact attttattaa actctaaaaa gttataatct atttcttgtc ttattttttt
     5581 ttaattcaat agtttttggc atccaaattc caccagccaa aaaacttgca ccaggttcaa
     5641 tatgtaaata gtaaccagca ttggctattt tctttccccc tttattaata cttgcaccca
     5701 tattggtttt gtagggtgtt ttgtttgggc taaaacgaac atctctgtta attctaaaaa
     5761 tacaattttt agcaattaag ggctcgaggg ttgtatcttt cttttttagc tttttaatta
     5821 tagcctcatt gaggttaata aaatctgctt tggtttcatc ataccaacct ctgttagcat
     5881 caaaccaaat tttattatta ttatttttta aagaactcaa aaactgtagt gtgttttttt
     5941 gaagcataga ttccatattt tatcagtcca ctaattgtat aaattcgtct tcatttaaaa
     6001 tcttaatagt attgattttt tttgcttttt ctaatttatt tcctgcgtct tcgccaacaa
     6061 ttaagtaatt taattttgca ctaacaagga tgatttgtta tttattcttt tttttattct
     6121 ttctcaactt ttcaagttca gaaacatcca acaaatcttt attccttgct gcaaataatt
     6181 tcatatctct taaaaaatca taaggaacaa tgttcattgt aacatccttt tcaattgcaa
     6241 agatgacttt gtttttttca gcgtcatcaa aagaaatttt tgaatgaaca aaagtaagaa
     6301 catcaatatc tgctgttggg gtacctatat tcgccttaaa tggcttagta aaatcttctt
     6361 catataatag agcaacttca ttttctgaat attgcataca aagcagggtt tgtataaaag
     6421 caagtttatt ttcattggta ggtgcaagcc aaacatctaa atcgtgggta tgtctgttat
     6481 atccataata gtttacagca taaccaccaa taagtaaata acgcaaatta ttttttgcag
     6541 cacaggaaag aaacacaata aattcaggat gtaaaatatc catgatgtac t
//
LOCUS       DAOQPK010000113         6589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_6687_length_6589_cov_3.956994, whole genome shotgun sequence.
ACCESSION   DAOQPK010000113 DAOQPK010000000
VERSION     DAOQPK010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6589
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6687_length_6589_cov_3.956994"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /gene="nadC"
                     /locus_tag="PLU36_06465"
     CDS             complement(<1..619)
                     /gene="nadC"
                     /locus_tag="PLU36_06465"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HMZ46430.1"
                     /translation="MQNFNEQLKHLVTQALQEDVGDGDHSTLSCIPANKKGKAVLKIK
                     QDGVLAGVSVAEKIFKQIEPSANFIYFKQDGESMLSGDKAFEVEASIHTILTCERLVL
                     NCMQRMSGIATLTKTYTEKLQGFHTQLLDTRKTTPNFRLLEKEAVRIGGGINHRFGLY
                     DMIMLKDNHIDYAGGLENAIDKAWLYATNYKPLLKIEVETRSMEDV"
     gene            777..1427
                     /locus_tag="PLU36_06470"
     CDS             777..1427
                     /locus_tag="PLU36_06470"
                     /inference="COORDINATES: protein motif:HMM:NF023521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HMZ46431.1"
                     /translation="MKTKFIALCSIVILCTSVNAQQKNGAYLSVNPLGILEVQAAYGA
                     GIGYRFSEQWEISSEYATLKPSTWMGEGKFTNIEGFRSVTAIKYSIDVNEVTKSKTFI
                     GAEFRYKKFSYDDMADFTNKTTGVTTKEYNYRNTTITKGFATLIGKQWDLGENSKWAL
                     ELTAGIGLRFKNVLRNGAPENSYVVPTNTGFAETPNYKDNTTGVYFPVGLRVMFRL"
     gene            complement(1502..1924)
                     /locus_tag="PLU36_06475"
     CDS             complement(1502..1924)
                     /locus_tag="PLU36_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46432.1"
                     /translation="MIKQKNVKGILVAFLIFALSNNIQSQTIIGSEVYGVSPIAPDKT
                     GASMVDENWGLLPSMQGNGVDMWGRNITNMQNVEEPIIVDSTNELTGTTSVDFDEEPP
                     PPPDDPEDIPLDGGTTVLLAIAAGLEYKRRKIKKPTDK"
     gene            complement(2245..>6589)
                     /locus_tag="PLU36_06480"
     CDS             complement(2245..>6589)
                     /locus_tag="PLU36_06480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ46433.1"
                     /translation="ATKLYFVQQPTASTTFNDISPAVKVHATDANNNLDSNYTTNVQI
                     TATGAVLRNSPVTQTTLGGLSTFSNLQFATAGTGVTLTATSGSLTNAVSNGFNITLGS
                     PIVALAQWNFEAIGFSGSAGTTASTRLNAGSTTANAGEKAASSVFEGVHSSSSTKWYT
                     YAGNGSNQSFSTDRFASGDYYQFKTSSYGYQNITISFDQTGSNGGPRDFKIQYSTDGS
                     SFTDLSGGTYQLTNDSWSLSGDPKSASSRTFDLSSVTALNNKENIYIRFTVTSSTAID
                     GGSIEVGGTGRVDNVIIKGEAATFTWNGAGDGTNWNDAANWDCNCGLAPTINNPVVIP
                     NGANVVLNTDYTARNLQLQSTSSLKVAPNKTLTVEGVADFNGQHVTIQSDATGTGMIG
                     QVSGFIEDANNVTVERYISASGNRAYRLLTPSVTTSNYIRDNWQEGTNNPDVATNNNP
                     NPGYGTHITGAGGSSNGFDPTQNNQVSLYKLVTNAWVAVSNTNSTILEADKAYLLFVR
                     GSRDNINTINTLTGSSNTTLRATGTIKQGGQTFSSLPASSFSLVTNPYPSPLNWGSVY
                     TANSSNFENYVYIWDPNIGVRGGYVAVEDDGTTSVASALTQNLQSGQAFFVRTKSGIA
                     SLAITEADKASVNNLNVYKTSETAEKLNIQLRFTKDGKDVLADGVLARFASNYNNVVD
                     GNDAIQITNWDEDIALVRNSSNLSIESRNTISAADTLFLKMANLRVAQGTYNWIMAPT
                     NFNNNNLQAYLEDAFTGTRKSISLIDTNRIAFTVSNNVASTASNRFRIVFAERKIYYS
                     KSNNSNANELSSWSSTTDGNGTTPSSFNELANFVVQQGHTLQLADTLHLTEGTLTIKP
                     NAEINNNGLLSVAYNIENNGTVSGNGLLQLNGSIKQYFTGYGTYSKVELDNSNGAEIF
                     AEAQVNITTAYKPTNGILTTNNNLVFKSSAQKTASISSGNTTGNYINGNITLETYVPA
                     KAVRKWSFISSPLTQSIANGWQQQIHITGAGTGGSVCPTLLPHNNGFDATLTNASNIY
                     TYDASKASGQRWAALPTTTTDFTNAGKAFRINIRGNRNLGCSLLDGTINAPSEVTVTT
                     TGLLNNEAKNAGNFSIEFANNGVNNYVLIGNPYPSAISFSSLYSQNSTSINNNYALYI
                     PTNNAGVYTYWDGLTNTFTGGVGYDDITGNNIATAQAVFVQSAVAGNIVLHFSENIKT
                     EENATAYFKNNSVKEKIKINYLNSSGNKIDETVVRFISDNAVTNNEVGRLDIPSINTG
                     TFIATIKNNTQLAVHSRALNNLISDEVWLNIGATQTGNYTLHFSDYQTLEDADIFLED
                     HYLNTKQNIKLNDNYSFSVDVNNAATKGSARFSLVFIRTAPSEVVYNQIKMYPNPADK
                     QVSLLLPKGIDNGINYQIKLTDITGKIVIQRKAKGGKEVIDIASLTAGIYIVEITDSK
                     GKRVTEKLVKN"
BASE COUNT         1970 a         1179 c          988 g         2452 t
ORIGIN      
        1 ctacatcttc catacttctg gtttcaactt ctatttttaa taatggttta tagttggttg
       61 cataaagcca agctttgtca atagcatttt ctaatccacc tgcataatca atatggttat
      121 ctttaagcat aatcatatcg tacaaaccaa acctatgatt aatgccacca ccaattctta
      181 ctgcttcttt ttctaaaagc ctaaaattgg gtgttgtttt tctggtatct aacaattgtg
      241 tatgaaagcc ttgtaatttt tcggtataag tttttgtaag tgtagcaatg ccactcattc
      301 tttgcataca gtttagtacc aatctttcgc aggttaatat ggtgtgaatt gatgcttcta
      361 cttcaaaggc tttatcgcca cttagcatac tttctccatc ttgtttaaaa taaataaaat
      421 ttgccgatgg ttctatttgt ttaaatattt tttctgcaac agaaacacct gctaaaactc
      481 catcctgttt aattttcaac acagcttttc cttttttatt tgcaggaata cagcttaaag
      541 tgctgtgatc tccatcgcct acatcttctt gtaatgcttg tgtaactaag tgttttaatt
      601 gctcattaaa attttgcata gtacaaagta atagtattat atgtaatcct ctttaatcac
      661 ttttactaac aaaaacatct ttctaaagct tatgtatatc ccaaaaagac atttttattt
      721 ttttatttgc atctttttgg agcatcttta cgtctatgct gtatattccg tacaaaatga
      781 aaacaaaatt cattgcatta tgctctattg ttatactgtg tacctcagta aatgcccaac
      841 aaaaaaatgg tgcatacctc tctgttaatc cacttggaat attagaagtg caagctgctt
      901 atggtgcagg cattggttat agatttagtg aacaatggga aatttcttct gaatatgcta
      961 ctttaaaacc aagcacatgg atgggcgaag gcaaatttac caatatagaa ggttttagaa
     1021 gtgttactgc cattaaatat tctattgatg taaatgaagt taccaaaagc aaaactttta
     1081 ttggtgctga atttagatat aaaaaattta gttacgatga tatggcagat tttaccaata
     1141 aaactactgg agtaactact aaagaatata attatagaaa tactactatt acaaaaggct
     1201 ttgctacttt aattggcaag caatgggatt taggcgaaaa cagcaaatgg gctttagaat
     1261 taactgctgg tattggttta cgttttaaaa atgttttaag aaatggtgct cctgaaaatt
     1321 cttatgtagt gcctaccaat acaggttttg cagaaactcc aaattataaa gacaatacaa
     1381 ccggtgtata ttttcctgtg gggctaagag taatgtttag attataagca ttacaaccgc
     1441 aaccaaacaa aacagcattt ctattttttt taactcagaa aagctatcat aaaaaaacca
     1501 actatttatc agttggtttt tttatttttc ttcgtttgta ttccaatcct gcagcaattg
     1561 ctaataaaac ggttgtacct ccatctaatg gaatatcttc aggatcatct ggtggtggtg
     1621 gtggttcctc atcaaaatca acactcgttg tacctgttaa ttcattagta ctatctacta
     1681 taattggttc ctctacattt tgcatattcg taatatttct gccccacata tctacaccat
     1741 taccttgcat acttggtaat aagccccaat tttcatctac catagatgct cctgttttat
     1801 ctggtgcaat tggcgatacg ccataaactt cactacctat tattgtttgg ctttggatat
     1861 tattacttaa tgcaaaaatt aaaaaggcta ctaatattcc tttgacgttt ttctgtttta
     1921 tcattacatt ttttaattcg ggtacacaat tattttttat ttttttattt cattttgaac
     1981 gaagtgaaac atctacctgg ctttctttat gtcatgttga acaaagtgaa acatctttac
     2041 ttgtctgtca ttccgagcct gagaggaatc tcattgttct taacctttag atccttcgct
     2101 tacgctcagg atgacaaaaa gagactcagg atgacattca taattctaaa ttcttaactc
     2161 ataattctta actcataatt cttaactcat aattcttaac tcataactca taattcttaa
     2221 ttcttaattc ttaattctta actcttaatt ctttaccaat ttttctgtta ctctttttcc
     2281 tttactgtct gttatttcta caatataaat ccctgctgtt agtgatgcaa tatcaattac
     2341 ctctttacct ccttttgctt tgcgttgtat cactattttg cctgttatat ctgttagttt
     2401 tatttgataa tttataccat tatctatccc ttttggcaac aataaactca cttgtttatc
     2461 tgctggattg ggatacattt taatttggtt gtacaccact tcgctgggtg ctgttctgat
     2521 gaatactaaa ctaaaccttg cagagccttt tgtagcagca ttattaacat ctacactaaa
     2581 actataatta tcgtttaatt tgatattttg tttggtgttt aaataatgat cttctaaaaa
     2641 aatatctgca tcttctaatg tctgataatc gctaaagtgt aatgtataat tgcctgtttg
     2701 ggttgcacca atatttaacc aaacctcatc gctaattaaa ttatttaatg ctctggaatg
     2761 tactgcaagc tgtgtattat ttttaattgt tgcaataaat gttcctgtat taatagaagg
     2821 tatatctaac cttccaactt cattattagt aactgcatta tctgaaataa atcgtacaac
     2881 tgtttcatct attttattac cactgctatt caaataattt attttaattt tttcttttac
     2941 tgaattgttt ttgaaatatg ctgttgcatt ttcttctgtt tttatattct cagaaaaatg
     3001 taaaactata ttacctgcca ctgccgattg tacaaatact gcttgagcag tagctatatt
     3061 attacctgtt atatcatcat aacctacacc acctgtaaat gtatttgtta agccatccca
     3121 ataagtgtaa actcctgcat tattggtagg aatatatagt gcataattat tgtttatgct
     3181 ggtagaattt tggctatata aactactaaa gctaattgca cttgggtaag gattaccaat
     3241 taacacatag ttatttaccc cattgtttgc aaattcaata ctaaagtttc cggcattttt
     3301 tgcttcgtta tttaacaaac cggttgtagt tacagttact tctgatggtg catttattgt
     3361 tccatctaat aaactgcagc ctaagtttct attacctcta atatttatcc taaaggcttt
     3421 gcctgcattg gtaaaatctg tagttgtagt tggtaatgca gcccatcttt ggccacttgc
     3481 tttagatgca tcataagtat atatattaga tgcattggtt aatgtagcat caaaaccatt
     3541 attatgtggt aataaagtag ggcaaacaga accacctgtt cctgcacctg ttatatgtat
     3601 ttgttgttgc caaccatttg ctatactttg tgttaaagga cttgatataa aactccattt
     3661 tcttacagct ttagcaggta cataagtttc tagtgttata tttccgttga tataatttcc
     3721 agtagtattt cctgaactta tacttgctgt tttttgtgca gagcttttaa atactaaatt
     3781 attattggtt gtaagtatgc cattagttgg tttatatgca gtagtaatat ttacttgggc
     3841 ttctgcaaaa atttcggcac cgttgctatt atctaattct actttgctgt aagtgccata
     3901 gccagtaaaa tactgtttaa tactaccatt taattgtaat aaaccattac ccgaaacagt
     3961 tccattattt tctatattat aagcaacact tagtaaacca ttattattaa tttctgcatt
     4021 gggtttaatg gttaatgtac cttcagttaa atgtaaggta tctgcaagtt gtagtgtatg
     4081 tccttgctgt acaacaaagt ttgctaactc attaaaagaa gaaggtgttg taccattacc
     4141 atctgttgta ctactccaac tacttagctc atttgcattg ctattattag atttgctata
     4201 atatattttc ctttctgcaa aaacaattct aaatctattg cttgcagtag atgctacatt
     4261 gttagaaact gtaaatgcta tacggttagt atctattaag ctaatggatt ttctggttcc
     4321 tgtaaatgca tcttctaaat aggcttgcag attgttgttg ttaaaattag ttggagccat
     4381 tatccaattg taagttcctt gtgctacacg taaattggcc atttttaaaa acaaagtatc
     4441 cgctgcagaa atagtatttc tgctttctat actaagatta gaactatttc ttaccaatgc
     4501 tatatcttca tcccaattag taatttgaat ggcatcgtta ccatcaacaa cattgttgta
     4561 attacttgcg aatcttgcta aaacaccatc agccaataca tctttaccat ctttagtaaa
     4621 acgcaactga atgtttaatt tttctgcagt ttcagaagtt ttatatacat tcaaattatt
     4681 tacactggct ttatctgctt ctgttatggc taagctggca atacctgatt tagtacgaac
     4741 aaaaaatgct tgtccgcttt gtaggttttg tgttagggca gatgctacac tggttgttcc
     4801 atcatcttct acagcaacat aaccacctct taccccaata ttaggatccc atatatacac
     4861 ataattttca aagttgctgc tgttagcagt ataaactgaa ccccaattta aaggagatgg
     4921 ataaggattg gttactaaac taaaagacga agctggtaag ctactaaatg tttggccacc
     4981 ttgctttatg gtgcctgttg ctcttaaagt agtgttacta ctgcctgtta aggtattaat
     5041 agtattaata ttatctctac tacctcgtac aaaaagtaaa taagctttat ctgcttctaa
     5101 aattgtactg tttgtattgc ttactgctac ccaagcattt gttactaatt tgtataaaga
     5161 aacctggtta ttttgtgtag ggtcaaagcc attgcttgaa cctcctgctc ctgtaatatg
     5221 tgtaccataa cctggattag gattattgtt ggttgcaaca tctggattat tggttccctc
     5281 ttgccaatta tctctaatat aattactggt tgttacactt ggtgttaata aacgataagc
     5341 cctgttgcca gatgcactaa tgtatctttc tactgtaacg ttatttgcat cttctataaa
     5401 accacttact tgtcctatca ttcctgtacc tgttgcatcg gattgtatag ttacatgctg
     5461 accattaaaa tctgcaacac cttctacagt taatgtttta ttaggtgcaa cttttaaact
     5521 acttgtagat tgtaactgta aatttcttgc agtataatct gtatttaaaa ctacattggc
     5581 accattgggt attacaacag ggttattaat tgttggagct aaaccacaat tacaatccca
     5641 gttggctgca tcattccaat tagtaccatc tcctgcacca ttccaggtaa aagtagctgc
     5701 ctctcctttt ataattacat tatctactct tccagtgcca ccaacttcaa tactacctcc
     5761 atcaatagct gttgaggaag taacagtaaa tcttatgtaa atattttctt tattatttaa
     5821 tgctgtaaca ctgcttaaat caaaagttct tgaagatgct gattttggat ctcctgagag
     5881 tgaccagcta tcattagtaa gttgatatgt tccaccagat aaatcagtaa aactgctacc
     5941 atctgtacta tattgtattt taaaatctct tgggcctcca ttgctgccag tttggtcgaa
     6001 actgatggta atattttgat acccataaga acttgtttta aattggtaat aatcgccaga
     6061 agcaaatcta tctgtactaa atgattgatt tgaaccatta ccagcatatg tgtaccattt
     6121 tgttgaagaa cttgaatgaa ctccttcaaa aacagaactt gcagcttttt caccagcatt
     6181 ggcagtggtt gaacctgcat taagtctggt acttgctgta gtacctgcag aacctgaaaa
     6241 accaattgcc tcaaaattcc attgtgctaa agcaacaata ggagaaccta atgtaatatt
     6301 aaacccatta cttacagcat tagttaaaga accagatgtt gcagttaaag ttactccagt
     6361 gccagcagtt gcaaactgta agttactaaa agtagataac ccacctaaag tagtttgtgt
     6421 tacagggctg tttcttaata ctgcaccagt agcagtaatt tgaacattgg ttgtgtaatt
     6481 cgaatctaag ttattattag catcggtagc atgtacttta acagcaggtg atatatcgtt
     6541 aaatgttgta ctagctgtag gttgttgtac aaaatataat tttgttgct
//
LOCUS       DAOQPK010000114         6550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_6735_length_6550_cov_4.274673, whole genome shotgun sequence.
ACCESSION   DAOQPK010000114 DAOQPK010000000
VERSION     DAOQPK010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6550
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6735_length_6550_cov_4.274673"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="PLU36_06485"
     CDS             <1..772
                     /locus_tag="PLU36_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ46434.1"
                     /translation="KLAENTSLLSKRPFRSPHHTISDVALVGGGGIPQPGEISLAHNG
                     VLFLDELPEFKRTVLEVMRQPMEERKVTISRAKVAVDFPANFMLIASMNPCPCGFYNH
                     PEKECTCPAGAVQKYLNKISGPLLDRIDLHVEVTPVAFNELSNHKVIGESSEQIRNRV
                     IAARELQAQRYQQHTGIYANAQITSKMLREICVLDNVSEALLKKAMERLNLSARAYDR
                     ILKVARTVADLATSSSIKPEHIAEAIQYRSLDRESWAG"
     gene            827..2368
                     /locus_tag="PLU36_06490"
     CDS             827..2368
                     /locus_tag="PLU36_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46435.1"
                     /translation="MFTNKILITITISIFSCITNINAQKIAYSEPDKEEARNGNFEII
                     GKLNNQFLIYKNFRNSYYIYSYNNQMELVEKNKLDFLPDRIIDANFITYQSFAYMFYQ
                     YQKRNIIYSMGIKIDANGKNISEPIQLDSTEVNFNTNNKIYTILVSENKQKIMSLKIN
                     NKSEKYHIIKTILLDNNLNLTEENFTSIDMPERNDFLTGFQLDNDGDLVFVRAAGNAQ
                     NNNINHLSLITKHANSNQLTTSILPIKNLYLDNITVKIDNYNKRYLTTSFFSKQKRGN
                     IEGLYCYVWDKTNSKAIMNNTSTFSEELRNEAKGESTTKSAFNDYYLKNIITRIDGGF
                     IVIAESIYSSYRGGMNNRWDNWNNSPYWGGMSGGYYTYGYASRMGSPWYPWGDNNSFN
                     NITRYYADNILMLSYDSSAKIDWTNVIIKSQYDDDSDEKLNYTAINTGTDIKFLFNLQ
                     ERRNWMLNEQAISNEGQISRSPTLKNLERGYEFMPKFAKQVGSKQIIVPCLYRGYVCF
                     AKIDL"
     gene            2438..3352
                     /locus_tag="PLU36_06495"
     CDS             2438..3352
                     /locus_tag="PLU36_06495"
                     /inference="COORDINATES: protein motif:HMM:NF040411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6427 family protein"
                     /protein_id="HMZ46436.1"
                     /translation="MHFHFFIAPPQLLINSNDGFISTALHNIQTQTNSTVLFILYLLS
                     VFIQAIRLNYALNNFKMFQQPHQTVAMSYILFSGFFVQWCVLSAAIIANFLVIWIFIK
                     LAQLSSNTNPKTLLFNIGLITSCTILAYHPTYIIVVVVLFALAIVRSFKLVEWVILLM
                     GLAVPFYFLASWLYLTNQFHLIQNYLPDIHFHLPTKHFNYEIGFSSGAILLILIVGLY
                     FNSIHNARMIIQIRKNWSVLLIMLILFLPVSFIFGNSTLEASFLSIIPLSAIAANAYS
                     YPKRLLVSNFLFWLSILITLYINRLLIL"
     gene            3562..4287
                     /gene="ric"
                     /locus_tag="PLU36_06500"
     CDS             3562..4287
                     /gene="ric"
                     /locus_tag="PLU36_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HMZ46437.1"
                     /translation="MHISEESIVGELVAKDYRTAAVFKQNKIDFCCNGNRTITNACEQ
                     KKIDSKQLIEQLIEATTTNTTNNSEFNSWPIDLLADYIEKKHHRYVETKIKEIMPFLH
                     KVVRVHGERHPELAEVEGLFVECAEELTEHMQKEEMVLFPFIREMVEAKHNGTNVKSA
                     FGTIQNPIGMMMHEHDAEGERFRKIAALTNNYNPPIDACSTYRVTFSLLKEFEDDLHL
                     HIHLENNILFPKSIQLETQFSAS"
     gene            4332..4868
                     /gene="azu"
                     /locus_tag="PLU36_06505"
     CDS             4332..4868
                     /gene="azu"
                     /locus_tag="PLU36_06505"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HMZ46438.1"
                     /translation="MKKSIVVLASITFLLTACSNSGSETKTEATTEATTEAATEAPAE
                     ETPGEAPAATDGTVTLTGGDDMKFDLSEIKVKDGQTVKLTLVHSGKMPKTAMGHNFVL
                     LATGVDVAKFATDAINAKDNDYIPASHKKDIIAHTKTIGGGESTTIEFKAPAKGTYDF
                     LCSFPGHSAMMKGKFIVE"
     gene            4919..5512
                     /locus_tag="PLU36_06510"
     CDS             4919..5512
                     /locus_tag="PLU36_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMZ46439.1"
                     /translation="MQIDYNILIAYGGVAKKFAKGEYVFIEDAMPNFYYQVVEGEVKV
                     YSSNSNGKEIIQGIFTEGQSFGEPPLLIDKPYPSSAIAITPLVVIRLSKEKLNNILKD
                     FPEISHSFIYTFAERMYNKASIALIRIGTNPEEIIEQFLYKYKEEHKKDNKPMLIPYT
                     RQQIADFTSLRVETVIRTLRKMSNHKIVQIIDHKLYY"
     gene            5517..>6550
                     /locus_tag="PLU36_06515"
     CDS             5517..>6550
                     /locus_tag="PLU36_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46440.1"
                     /translation="MNNTYNLKNWFKLGLISLIIVALYGTTMRYKIAFNFPYLEQKNI
                     MHAHSHFGFIGWLSHIIYCGLALNIFPFITKIKQKKYIILIAANLACALGMLISFTLY
                     GYRISSIAFTSLTILINIIFTIAYIFDSKFFPKNNPSKYWAITGLLLNIVSAAGPLSL
                     AYIMTNKVINFNFYLGSVYYYLHFQYNGWFFFGGMAMVSSILPLEFPSIKKYYYAFAI
                     TAIPTFFLSILWAKLPLWLYIITVIATIIQFFAWVMLVYKGYQFLKNKEITTRPKWVN
                     ILFYAAAFALTIKFTLQTISVIPSLSQMVYGFRPIIIAYIHLVLLGVFSLFILGYLFQ
                     KQIFSPSASL"
BASE COUNT         2365 a          933 c          993 g         2259 t
ORIGIN      
        1 taaacttgca gaaaatactt ctttactttc taaacgccct tttagaagtc cacatcatac
       61 cattagtgat gttgcacttg ttggtggtgg cggaatacca caacctggcg aaatttcttt
      121 ggctcataat ggagtattgt ttttagatga acttccagaa tttaaaagaa ctgttctaga
      181 agtaatgagg cagccaatgg aggaaagaaa agttactatt tccagagcta aagttgctgt
      241 tgattttcct gcaaatttta tgcttattgc aagtatgaac ccctgccctt gtggctttta
      301 caatcatccg gaaaaagaat gtacttgccc ggctggtgca gtgcaaaaat atttaaataa
      361 aataagtgga ccattattag acagaattga tttgcatgta gaagtaacac cagtagcatt
      421 taatgagtta agcaaccata aagttattgg cgaaagttca gaacaaataa ggaacagagt
      481 tatagcagct agagagctac aagcacaaag ataccaacaa catacaggca tttatgctaa
      541 tgcacaaata accagtaaaa tgttaagaga aatttgtgtg ttagataatg ttagcgaagc
      601 attgttaaaa aaagcaatgg aaaggttaaa cttaagtgcc agagcttatg atagaatttt
      661 aaaagtagct cgtacagttg cagatttagc aacaagcagt tcaataaaac cagaacatat
      721 tgcagaagct atacaatata gaagcctgga cagagaaagt tgggcaggat agttgttatg
      781 ttcaaataaa tttaaactat tacatttgaa atttaaagtt acatgtatgt ttacgaataa
      841 aattttaatt acaattacta tttcaatttt tagttgtata actaatataa atgctcaaaa
      901 aattgcttac tcagagcctg ataaagaaga agcaagaaat ggcaattttg aaataattgg
      961 caaactaaac aatcagtttt taatatataa aaactttaga aatagttatt acatatattc
     1021 ttacaacaac caaatggaat tagttgaaaa aaacaaatta gactttttac ccgatagaat
     1081 tattgatgcc aactttatta cctaccaaag ttttgcgtat atgttttacc aatatcaaaa
     1141 aagaaatatc atatactcta tgggtattaa gatagatgcc aatggtaaaa acatttcgga
     1201 acctatacaa cttgattcaa cggaagtaaa ttttaatact aataataaaa tttataccat
     1261 tttggtaagt gaaaacaagc agaaaataat gagcctaaaa attaataata aaagcgaaaa
     1321 ataccatatt attaaaacca ttttattgga taataaccta aaccttacag aagaaaactt
     1381 caccagtatt gatatgcccg aaagaaatga ctttttaaca ggttttcaac tagacaatga
     1441 tggggattta gtatttgtta gagcagcagg taatgctcaa aataataaca ttaatcattt
     1501 atccttaatt accaaacatg ctaatagtaa ccagttaact acaagtattt tacccataaa
     1561 aaatttatac ctagataata ttactgtaaa aattgataat tataataaaa gatatttaac
     1621 cacttctttc tttagtaaac aaaaaagagg caatatagaa ggcttgtatt gttatgtttg
     1681 ggataaaact aatagtaaag ctattatgaa taacacttct actttttctg aagaattaag
     1741 aaatgaggct aaaggagaga gtaccacaaa atcggcattt aatgattatt atttaaaaaa
     1801 tattattaca agaatagatg gtgggtttat agtaattgca gaatctattt attcctccta
     1861 cagaggtggc atgaacaaca gatgggataa ttggaacaac tctccatatt ggggaggtat
     1921 gtctggtggt tattatactt atggatatgc tagtagaatg ggttctcctt ggtatccttg
     1981 gggcgataat aatagcttta ataatattac acgatactat gcagataata ttttaatgtt
     2041 aagctacgat tcttctgcca aaatagattg gactaatgtt attattaaat ctcaatacga
     2101 tgatgactct gatgaaaaac ttaattatac agcaataaat acaggtacgg atataaaatt
     2161 tttatttaat ttacaagaac gaagaaattg gatgcttaat gaacaagcta tttctaatga
     2221 aggtcaaata tctcgtagcc ctactttaaa aaatttagaa cgtggttatg agtttatgcc
     2281 aaagtttgca aaacaagttg gttccaaaca aataattgtt ccttgccttt atagaggtta
     2341 tgtttgcttt gctaaaattg atttataata aatggtattt ctctataaag acaagtcagt
     2401 atctggtatt tttttcttac tgcttttgct tattgcagtg cattttcatt tttttattgc
     2461 tcctcctcag ttattaatca attccaacga tggttttata agtactgctt tacataatat
     2521 tcaaactcaa accaattcta cagtactttt tatcctctat ttgttatctg tatttataca
     2581 agcaattcga ttaaactatg ctttaaacaa ttttaaaatg tttcaacagc cacatcaaac
     2641 agtggctatg agttatattt tattttcagg tttttttgta caatggtgtg tattaagtgc
     2701 tgctattatt gcaaactttt tagttatttg gatatttata aagttagcac agttaagctc
     2761 taatactaac cccaaaactt tattatttaa tattggcctt attacaagct gtactatttt
     2821 ggcatatcat ccaacatata ttatagttgt tgtagtatta tttgctttag ccattgttcg
     2881 ttcttttaaa ttagttgaat gggttatttt attaatgggg ttagcggtgc ctttttactt
     2941 tttagcatcg tggctttatt taaccaatca atttcatctt attcaaaatt atttacccga
     3001 tattcatttt catttaccaa caaaacactt taattatgaa ataggattta gtagtggtgc
     3061 aattctttta attttaattg ttggtttata ttttaatagc attcataatg ccagaatgat
     3121 tatacaaata agaaaaaact ggagtgtatt gttaataatg cttatacttt ttttacctgt
     3181 atcctttatt tttggcaact ctacattaga agcctccttt ttaagtataa tacccttgtc
     3241 ggcaattgct gctaatgcat atagttaccc aaaaagatta ttagtttcta attttttgtt
     3301 ttggctaagc attttaatta ctttatacat caataggctg ttgatacttt aagtaaagcc
     3361 aatttcggaa ttggtaattt tttgcatttt taaacattta agtttgttaa aaagtaaaaa
     3421 tatttccgat actcatatct taaataaaaa ctactcatat ctttgcagcc ttaaaaaaag
     3481 aaataatcat aataagtgta tttctaagta ttataacatt tattattctt tttattattt
     3541 tattctaaaa aatataatat tatgcatatt tcagaagaat ctattgttgg cgaattagta
     3601 gcaaaagatt acagaacagc agcagtattt aagcaaaaca aaatagactt ttgctgtaat
     3661 ggtaacagaa caattaccaa tgcctgcgaa caaaaaaaaa ttgatagtaa acagttaatt
     3721 gagcaactaa tagaagctac cactactaac actactaaca attctgaatt taactcttgg
     3781 ccaatagatt tattagcaga ttatattgaa aagaaacacc atagatatgt agaaacaaag
     3841 attaaagaga taatgccttt tttacataaa gtagtaagag ttcatggaga aagacatcca
     3901 gaacttgcag aagtggaagg attatttgta gaatgtgctg aagaattaac agaacacatg
     3961 caaaaggaag agatggtatt atttccattt attagagaaa tggtagaagc aaaacacaat
     4021 ggaacaaatg taaaatctgc ttttggtaca atacaaaacc ctattggaat gatgatgcat
     4081 gaacacgatg ctgaaggaga acgctttaga aaaattgcag cattaaccaa taattacaat
     4141 ccacctattg atgcttgtag tacttatcgt gtaacatttt ccttactaaa agaatttgaa
     4201 gatgacttac atttgcatat tcatttagag aacaatattt tgtttcctaa gtcaattcaa
     4261 ttagaaacac aattttcagc atcttaatca ttaaaaagct aattattaat cacacataaa
     4321 caaaaagaaa aatgaaaaaa tcaattgttg tgttagcctc tattacattt ttgctaacag
     4381 catgttcaaa ctcaggtagt gaaacaaaaa cagaagctac aacagaagct acaacagaag
     4441 ctgcaactga agcaccagca gaagaaacgc caggagaagc accagctgca acagatggta
     4501 ctgtaacttt aacaggtggc gatgatatga aattcgactt atctgaaatt aaagtaaaag
     4561 acggacaaac tgtaaaatta actttagtgc actccggtaa aatgcctaaa acagctatgg
     4621 ggcataactt tgttttatta gcaacaggtg tagatgtagc taaatttgct acagatgcta
     4681 ttaatgcaaa agataacgat tatatccctg catctcacaa aaaagatatc attgctcata
     4741 ctaaaactat tggtggtggc gaatcaacaa ctatagaatt taaagcacct gcaaaaggca
     4801 cttacgattt cttatgttca tttcctggcc attctgcaat gatgaaaggt aaatttattg
     4861 tagaataaga gccgataact cagttttttt tgttattatt atagtttaaa actaattaat
     4921 gcaaatagac tataatatac taatagcata tggtggcgtt gccaaaaaat ttgcaaaggg
     4981 tgaatatgtt tttattgaag atgctatgcc taatttttat tatcaggttg tagaaggcga
     5041 agtaaaagtg tattcctcca actccaatgg taaagaaatt attcaaggaa tttttaccga
     5101 agggcaaagt tttggcgagc caccattgtt aattgataag ccttacccta gctcggcaat
     5161 tgccattacg ccattagtgg taattagact ttctaaagaa aaactaaata atattttaaa
     5221 ggattttcca gaaatatcac attcttttat ttataccttt gcagaaagaa tgtataataa
     5281 agccagcatt gctttaatta gaattggtac caacccagaa gaaattattg agcaattttt
     5341 atacaagtat aaagaagagc ataaaaaaga taataaacca atgctaatac cttatacaag
     5401 acaacaaatt gcagatttta ccagccttag ggttgagaca gttatacgta ctttaagaaa
     5461 aatgagcaac cataaaattg ttcaaataat agatcacaaa ttatattatt aatataatga
     5521 acaacaccta caacctaaaa aattggttta aacttggttt aattagttta attattgttg
     5581 cattatatgg tacaaccatg cggtataaaa ttgcttttaa ttttccgtat ttagagcaaa
     5641 agaatattat gcatgcccat tctcattttg ggtttattgg ttggctttct catataattt
     5701 attgtggttt ggctttaaat atttttccat ttattactaa aataaaacag aaaaaatata
     5761 ttatacttat tgctgcaaat ttggcttgtg cattaggtat gcttatttct tttacattat
     5821 atggttatag aatttcatct attgcattta ctagtttaac cattttaata aatattattt
     5881 ttactatagc atatattttc gatagtaaat tttttcctaa aaataatcct tcaaaatatt
     5941 gggcaattac aggtttatta ttaaacattg tttcggctgc aggcccatta agtttggcat
     6001 atataatgac caataaggtt attaatttta atttttattt aggctcggta tattattatt
     6061 tacattttca atataatggc tggtttttct ttggtggtat ggcaatggtt tctagtattt
     6121 tacctttaga gtttccatcc ataaaaaaat attattatgc ttttgccatt actgcaatac
     6181 ctactttctt cttatcaatt ttatgggcta agttgccttt atggctttat attataacag
     6241 ttattgctac tataattcaa ttttttgcgt gggtaatgct tgtatataag ggctatcagt
     6301 ttcttaaaaa taaagaaata actacaagac ctaaatgggt taatatttta ttttatgctg
     6361 cagcttttgc tttaacaata aaatttacac ttcaaaccat ttcagttatt ccttcactaa
     6421 gccaaatggt ttatggtttt aggcctatta ttattgccta catacactta gtattattag
     6481 gtgtatttag tttatttatt ttaggttatt tatttcaaaa acaaattttc agcccttcgg
     6541 cttctttaaa
//
LOCUS       DAOQPK010000115         6546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_6744_length_6546_cov_4.318903, whole genome shotgun sequence.
ACCESSION   DAOQPK010000115 DAOQPK010000000
VERSION     DAOQPK010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6546
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6744_length_6546_cov_4.318903"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="PLU36_06520"
     CDS             <1..983
                     /locus_tag="PLU36_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HMZ46441.1"
                     /translation="FETVNGGTIFLDEIGEMPIGTQARLLRVLETGEFIRVGSSKVQK
                     TDVRVIAATNKELLEYTSKGKFREDLYYRLNTVPIRVPALRNRKEDILLLFRKFVVDF
                     AEKYKTVPIQLDEDAKNILTNYNFPGNVRELKNIAEQISVLSKTSFINSSQLNTFLPE
                     NKLNNMPVLANGFVSNGEFANEREILYKLFFDMKKDVTELKKMFLEILQNPSLASTAA
                     NFTKESILTDFSNTQATEVKQVNQQPQIVQPTFVQPIHSTNNQAVIINDEIQQHEVVE
                     ESLNIMDKEKELIIKALKKHKSRRKDASADLGISERTLYRKLKEYDIEDL"
     gene            1008..1523
                     /gene="lptE"
                     /locus_tag="PLU36_06525"
     CDS             1008..1523
                     /gene="lptE"
                     /locus_tag="PLU36_06525"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HMZ46442.1"
                     /translation="MHPSFSKNIRICFLYAFLFFLAACKIYSFKDVSIPPNIKTIKIG
                     FIENKARIVNPQLSPMLTDKIQQKITNQTRLTRTNSEDAHLQIEGYISDYSVTTTGVS
                     AQQEVTNRLTVSLQINLKNTLENSSKDFTITRSFDFSANLSLDQAQISLQEEIVRSLA
                     DDIFNRIFSNW"
     gene            1608..2708
                     /locus_tag="PLU36_06530"
     CDS             1608..2708
                     /locus_tag="PLU36_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46443.1"
                     /translation="MHIALRKIFHQFSGKTNLEDTRIELLFEAAENEPYFAPAQFFLA
                     LKMKSENHSNLSAQLNKTALYFNNIHWLHYQLVPKPELIEFIEKEKTETTQPILQNVI
                     QEDIKNHNSQINEDTNSEKVEDAVVENNIPLEAEITEAIIEKPIEFEKEKTDFVPKEN
                     QSITALVLSNLDNEEVLNPIQTIHTKNDVPMLDTNETDLNEDVQNEPDKTNRTEVELE
                     NKLSTILKEQLEEYQQPVTKESELQFEQSPHHTVDYFESQGIKAEINLNSNDKLSTQL
                     KKFTDWLKHMKNLNLEQEKDLGTDPELENAIQGIAQNSNEAREIVTETMAEVLEKQGK
                     KDKAIQLYIKLSFLNPDKSSYFAAKIQHLKGI"
     gene            2711..3001
                     /gene="secG"
                     /locus_tag="PLU36_06535"
     CDS             2711..3001
                     /gene="secG"
                     /locus_tag="PLU36_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HMZ46444.1"
                     /translation="MVLIFVILVVLASVALGFVILVQNPKGGGLSGNIGGIGNQFMGV
                     KQTTDMLEKGTWMFVSIIAVLAIVSSLLFKNVSSDSNLKKQIDKIDTRTPAK"
     gene            complement(3062..5257)
                     /locus_tag="PLU36_06540"
     CDS             complement(3062..5257)
                     /locus_tag="PLU36_06540"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HMZ46445.1"
                     /translation="MKKIIALLLVVMVCISSNVKAQHTIVTKVFNHENDEVLQGVLIK
                     IAKTNIRTVTNENGVGVLKNVPKGNFKIEFSYTGFESASVQVTVPQAKNDTLIVALHN
                     HEEELEEVFVQSTRTSRTIKNSPTRVETISGEELDEKNNMRPANVSMLIHESTGMQLQ
                     QTSATTGNASIRVQGLDGRYTQLLKDGYPNFGNFANGLSVLEIPPLDLKQVEIIKGPA
                     STLYGGGAIAGVVNFISKTPKEKLEGNFILNQSNIGQSNIAGYLQQKKGKFGFTVLAA
                     YNYSKAYDVDKDDFSEIPKSNNYTIHPRLFFYPDSKTTITIGNNLTQSNSKGGDMQVI
                     KGNSSLGHVYFEKNTTTRNTVTLEADRKINSDSRFYLKQSFSTFNRNLQTNNHEFAGL
                     STNSYTDLSYTFNKNNHTIISGLNIIYDDFKQHKVTNAFLNSKSFTTGFYAQDTWDVS
                     EIIKIEGGLRVDNVTYKNVNFSKNQTFVLPRVSVLLNYSNHLSSRIGAGLGYKVPTIF
                     TEETEAMHYNNLQALDNSVTSERSVGGTIDVNYKNKIGADFQYSFNIMYFLTSINKPL
                     ILEQNMQNLFVFKNETKPVISNGIEANIKFIYKNDLKLFVGYTLLDAKAIYKTVNNFI
                     ALTPKNKLNFVLMYEKHNNFKLGFEGYYSDKQYLSNGTKTPNFIELGFLAEKTWKKLT
                     VFINFENFTDERQSNYKRVVNAPNTNPTFDEIWNHTEGRVINGGIKIKL"
     gene            complement(5344..5661)
                     /locus_tag="PLU36_06545"
     CDS             complement(5344..5661)
                     /locus_tag="PLU36_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46446.1"
                     /translation="MKFITFIVAVCMLTFSVMPCYDTHDDCSTESLALVEHADNHTKD
                     SEDFCSPFCVCFCCRNLVLQQDEIKDLEVQPTIFYLASQSIYSTATIPTAFLNNIWQP
                     PKV"
     gene            complement(5766..6194)
                     /locus_tag="PLU36_06550"
     CDS             complement(5766..6194)
                     /locus_tag="PLU36_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HMZ46447.1"
                     /translation="MTLIKHYPKSINQMFDDLLMNFNETWKTEVTESNFMPQVNIHET
                     NEGYHVELNAPGRNKEEFKINVENNILTISFEKAEEKDVKEYKTIRREFNYETFKRSF
                     TLNEKMNIENIQAKYDNGILKLFVPKIEEVKILPKQITIQ"
     gene            6408..>6546
                     /locus_tag="PLU36_06555"
     CDS             6408..>6546
                     /locus_tag="PLU36_06555"
                     /inference="COORDINATES: protein motif:HMM:NF018229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HMZ46448.1"
                     /translation="MYPQELVLPMKAELTEEGFEDMKTANAVEETLKKQGTTLVVINS
                     VC"
BASE COUNT         2396 a          969 c          897 g         2284 t
ORIGIN      
        1 attttgaaac agtgaatggt ggtactatat ttttagatga aattggcgaa atgccaattg
       61 gtactcaagc cagattatta cgtgttttag aaacaggcga atttattaga gttggctctt
      121 ctaaagttca aaaaacagat gtaagggtta ttgctgctac caataaagaa ttattggaat
      181 atactagtaa aggaaagttt agagaagatt tatattatcg tttaaataca gtgccaattc
      241 gtgtacctgc attaagaaat agaaaggaag atatattatt actatttaga aaatttgttg
      301 ttgattttgc agaaaaatat aaaacagttc ctattcaatt agatgaggat gctaaaaata
      361 ttcttactaa ttacaacttt ccgggtaatg ttagagagtt aaaaaatata gcagaacaaa
      421 tttcggtatt aagcaaaact tcttttataa attcttctca actaaacact ttcttacccg
      481 aaaataaatt aaacaatatg ccagttttgg caaacggatt tgttagtaac ggcgaatttg
      541 caaatgaaag agaaatactc tacaagcttt ttttcgacat gaaaaaagat gttactgagt
      601 taaagaaaat gtttttagaa atcctgcaaa acccttcatt agcaagtact gcagccaatt
      661 tcactaaaga aagtatatta accgattttt ctaatacaca agccaccgaa gttaaacaag
      721 taaaccaaca gccacaaatt gttcaaccaa cttttgttca acctattcat tctactaata
      781 accaggctgt aattattaac gatgaaatac aacaacacga agtggtggaa gaatctttaa
      841 acataatgga taaagaaaaa gagcttatta ttaaagcatt aaaaaaacac aaaagcagaa
      901 gaaaagatgc cagtgcagat ttaggcatta gcgaaagaac tttataccga aagttaaagg
      961 aatacgatat tgaagattta taatttttta taccagaaat aatcaaaatg catccgagct
     1021 ttagtaaaaa tatacgtatc tgttttttat atgcttttct atttttttta gcagcatgta
     1081 aaatttattc ttttaaagat gtaagtattc ctcctaatat aaaaactatt aaaattgggt
     1141 ttattgaaaa caaagccaga attgtgaatc cacaattaag ccccatgtta acagataaaa
     1201 tacaacaaaa aattactaac caaaccaggc ttacaagaac caatagcgag gatgcacatt
     1261 tacaaataga aggctatatt tctgattatt ctgtaacaac aactggtgta agtgcacagc
     1321 aagaagtaac taatagactt actgtgagcc ttcaaattaa ccttaaaaat actttagaaa
     1381 atagtagtaa agattttaca attactcgta gcttcgattt ttcagcaaac ttatcattag
     1441 accaagcaca gatttctttg caagaagaaa ttgtaagaag tttggcagat gatattttta
     1501 atagaatttt ctctaattgg taattacatt ttaaactttt ttattttaat aaactagtaa
     1561 ataaaataaa tatctcgtgt atatttgttt tgtttatttt caatattatg catatagctc
     1621 taagaaaaat atttcatcaa ttttctggta aaactaattt agaagatacc cgaattgagt
     1681 tgctatttga agcagccgaa aatgaaccct actttgctcc tgctcagttt ttcttagctc
     1741 tgaaaatgaa aagcgaaaat cattccaacc tatctgctca attaaataaa acagctttat
     1801 atttcaataa tattcactgg ctacattacc aattagtacc caaacctgaa ttgatagagt
     1861 ttattgaaaa agaaaaaact gaaactacgc aaccaattct tcaaaatgta attcaagaag
     1921 atattaaaaa tcataatagt cagataaatg aagatactaa ttcagaaaaa gttgaagatg
     1981 cagttgtaga aaacaatatt cctttagaag ctgaaattac tgaagcaatt attgagaaac
     2041 ctattgaatt tgaaaaagaa aaaacagatt ttgtacctaa agaaaaccaa tctatcaccg
     2101 ctttagtatt aagtaattta gataacgaag aagttctaaa cccaattcaa actattcata
     2161 caaaaaatga tgtacctatg ttagatacaa atgaaacaga tttgaatgaa gatgtacaaa
     2221 atgagccaga caaaacaaat agaacagagg ttgaattaga aaataaatta tcaactattt
     2281 taaaagagca attagaggag tatcaacaac cagttacgaa agaaagtgaa ttacaatttg
     2341 agcaaagccc gcatcatact gtagattatt ttgaatcgca aggtataaaa gctgaaatta
     2401 atttaaattc taacgataaa ctttccacac aattaaagaa gtttactgat tggttaaaac
     2461 acatgaaaaa cttaaattta gaacaggaga aagatttagg caccgatcca gaacttgaaa
     2521 atgcaataca aggaatagct caaaactcta acgaagcaag agaaattgta accgaaacaa
     2581 tggcagaagt tttggagaaa cagggtaaaa aagataaagc cattcagtta tatataaaat
     2641 taagtttttt aaatcctgat aaatcctctt actttgcagc caaaattcaa catttaaaag
     2701 gtatataatt atggtattaa tttttgtaat cttagtggta ttagcaagtg tagctttagg
     2761 ctttgttatt ttagtacaaa acccaaaagg tggtggtttg tctggcaata ttggtggtat
     2821 tggtaatcag tttatgggtg taaaacaaac aaccgatatg ttagaaaaag gaacttggat
     2881 gtttgtaagc attattgcag ttttagcaat tgtatcctct ttattattta aaaatgttag
     2941 tagcgatagt aacctaaaaa agcaaataga taaaatagat actagaactc cggcaaaata
     3001 atttatttat ttaatttctt ttataaaaat agaggctgcc cataagcagc ctctattttt
     3061 attataactt aatcttaatg cctccattaa ttacccttcc ttcagtatga ttccaaattt
     3121 catcaaacgt tggattggtg tttggtgcat ttaccactct tttataattg ctctgacgtt
     3181 catcagtaaa attttcaaag ttgataaaca ctgttagttt cttccaagtc ttttctgcaa
     3241 gaaaacctaa ctcaataaag tttggagttt ttgtaccatt acttaaatac tgtttatctg
     3301 aatagtagcc ttcaaagcct aatttaaaat tattatgctt ttcatacatc aacacaaagt
     3361 ttagtttatt tttaggtgtt aaagcaataa aattgtttac tgttttataa attgcttttg
     3421 catctaacaa agtataacca acaaataatt ttaaatcgtt tttataaata aacttaatat
     3481 ttgcttcaat tccattacta attactggct ttgtttcatt tttaaataca aacaaatttt
     3541 gcatattttg ttctaaaatt aatggcttat taattgaagt taaaaagtac ataatattaa
     3601 aactatactg aaaatctgcc cctattttgt ttttataatt aacatcaatt gttccgccaa
     3661 cacttctttc agaagttaca ctattatcta aagcttgtaa attattatag tgcattgctt
     3721 ctgtttcttc tgtaaaaata gttggtactt tataccctaa accagcacca attctagaac
     3781 ttaaatggtt actataattt aataaaactg aaactcttgg taaaacaaaa gtttgatttt
     3841 tagaaaagtt tacattttta tatgtaacat tatcaaccct taaaccacct tctattttaa
     3901 ttatttcaga tacatcccaa gtgtcttgag cataaaagcc tgttgtaaaa cttttagaat
     3961 ttaaaaaagc attggttact ttgtgttgct taaaatcgtc gtaaataata tttaatccag
     4021 aaataatagt atggttattt ttattgaatg tataactcaa atctgtgtaa ctattagtac
     4081 ttaagcctgc aaactcatga ttattggttt gaagatttct attaaatgta ctaaaacttt
     4141 gttttaagta aaaacggcta tctgaattaa tttttctgtc tgcctctaaa gttactgtat
     4201 ttcttgtggt agtatttttt tcaaagtaaa catgcccaag agaactattg cctttgataa
     4261 cttgcatatc gccaccttta gaattacttt gtgtaagatt attacctatt gtaatggttg
     4321 ttttactatc gggataaaaa aataatcttg gatgtatagt ataattattt gacttcggaa
     4381 tttcagaaaa atcatcctta tctacatcat aagcctttga ataattgtaa gctgctaaca
     4441 cagtaaaacc aaatttacct ttcttctgct gtaaataacc tgcaatatta ctttgcccaa
     4501 tattcgactg atttaaaata aaattacctt ccagtttttc ttttggtgtt ttactaataa
     4561 aatttacaac accagcaatt gctccaccac catataaggt tgatgcaggt cctttaataa
     4621 tttctacttg ttttaaatct aatggtggta tttctaatac acttaacccg tttgcaaaat
     4681 tgccaaaatt aggatatcca tcttttagca attgagtata tctaccatct aaaccttgta
     4741 ctctaatact tgcatttcca gtagttgcac tggtttgttg taactgcatt ccagtacttt
     4801 catgtattaa catactcaca tttgcaggcc tcatattgtt tttctcgtct agttcttctc
     4861 cactaattgt ttcaaccctg gtaggagaat ttttaatagt cctacttgtt ctggtacttt
     4921 gtacaaatac ttcttctaat tcctcttcgt gattatgtaa tgctacaatt aatgtatcat
     4981 ttttagcttg tggcactgta acctgtacac tagcagactc aaagccagta tagctaaatt
     5041 caattttaaa attacctttg ggaacatttt ttaatactcc aaccccattt tcattggtaa
     5101 ctgttctaat atttgttttt gcaatcttaa tgagtacgcc ttgtaaaact tcgtcgttct
     5161 catgattaaa tactttagta acaattgtat gttgagcttt tacattgcta ctaatacaaa
     5221 ccattactac taatagtaat gctattattt ttttcataaa attctttaaa ttagtcttta
     5281 ataatattta aataatcaaa ttcaattgta tatattgaat atttgataat ggcagtaaaa
     5341 agattacact ttgggaggtt gccaaatatt atttaagaac gcagttggaa tagtagcagt
     5401 agaatatatg ctttgtgagg ctaaataaaa tatagtcggt tgcacttcta aatcttttat
     5461 ttcatcttgt tgaagtacta ggtttctaca gcaaaaacaa acacaaaatg ggctacaaaa
     5521 gtcttcacta tctttagtat gattatctgc atgttctacc agtgcaagag attcagttga
     5581 acaatcatca tgtgtgtcat aacatggcat tacagaaaat gtaagcatac aaactgcaac
     5641 tataaatgtt ataaacttca ttggttgcaa atataatgtt tcaaataaaa taaaaaaggc
     5701 cccaagtaga tacaaggagc cgttaaccaa aactaactgc ttatgaaact cttattttaa
     5761 aaaatttatt gaatagttat ttgcttagga agaattttta cttcttcaat cttaggaaca
     5821 aaaagtttta aaattccatt atcatattta gcctgaatat tttcaatatt cattttttca
     5881 tttaatgtaa agctgcgttt aaaagtttca taattaaact ctcttctaat ggttttatac
     5941 tcttttacat ccttttcctc tgctttctcg aaactgatag ttaaaatatt gttctctaca
     6001 tttattttaa attcttcttt atttctacct ggtgcattca actctacatg gtaaccttcg
     6061 ttggtttcat gaatgtttac ttgtggcata aaattgcttt cagttacttc tgttttccaa
     6121 gtttcattaa agttcattaa taaatcatca aacatttgat taattgattt tggatagtgt
     6181 ttaataagtg tcataattat aatttttatt ttttattgtt atgcataact attttcaaaa
     6241 gctataccaa ccctatattt atgaaaaaat gtacaattta gtaaaaagcg taagacattt
     6301 tgtcttaaat tatataaaat ataagacatt ttgtctttaa agacatataa gaaataaata
     6361 gaaaaaatgt atttttgcaa aaaatttaaa ataaaataat aaaaattatg tatccacaag
     6421 aattagtgtt accaatgaaa gccgaattaa cagaagaagg ctttgaagat atgaaaaccg
     6481 ccaatgctgt tgaagaaacc ttaaaaaaac aaggtacaac tttagtagtt ataaatagtg
     6541 tttgtg
//
LOCUS       DAOQPK010000116         6515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_6795_length_6515_cov_4.879257, whole genome shotgun sequence.
ACCESSION   DAOQPK010000116 DAOQPK010000000
VERSION     DAOQPK010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6515
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6795_length_6515_cov_4.879257"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PLU36_06560"
     CDS             complement(<1..549)
                     /locus_tag="PLU36_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMZ46449.1"
                     /translation="MKYPAIQNFINGKFVSASTTKTMDVISPLNGSHLSTVPMSAYND
                     LDNAVRAAKAAFPAWSKTPIKERVQVFFRYKTLLEKHLKELAELCTEENGKTYAESVA
                     EIEKCVELTEFATSLPQLIQGEILEVSKGVECRTEHAPLGVVASIVPFNFPSMVPNWT
                     IPNAIALGNCMIIKPSEKVPLSC"
     gene            711..1175
                     /locus_tag="PLU36_06565"
     CDS             711..1175
                     /locus_tag="PLU36_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HMZ46450.1"
                     /translation="MSQHSLDTLDFNILSCLQQDGRMSFTVMAEKLNVSIATVRTRVN
                     KLIEDGTINIIGRVNPEKVGFHAYAHIAVYVRPATLKEKVANKISKYKEVSFLALTSG
                     DYDLEVDVMCKNNDFLVEFVNKISTIEGVHQTKTTIYFKVYKYAQPDLDLLR"
     gene            1248..1646
                     /locus_tag="PLU36_06570"
     CDS             1248..1646
                     /locus_tag="PLU36_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46451.1"
                     /translation="MIKFNELQTGDFVMVEYEGKMWEGEVTNLNHDEKQVEVTTEVQP
                     FWYAPESLHAVPLNDQQLMNFNFTKQVNEDGSIKYMKEAFRLVIPSKDDFSTIEMWYR
                     EDRRHHPDVHFMHQLQNHYYSMTKVHLTKS"
     gene            2153..>6515
                     /locus_tag="PLU36_06575"
     CDS             2153..>6515
                     /locus_tag="PLU36_06575"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HMZ46452.1"
                     /translation="MKKFLFFFCFLLLAIGSTIAQPMWSIATCSNLTSTTYGPMYSTS
                     TANATNRTAIIYPATQLVGVANTTLTSMFIKRATASGSMLGTPNFKIYLLETSSTEWG
                     AGSLDWATATGPATLVYDGNPVSAVGSSAGFKEFSLTTTFAYGTQNLAVFFEYTNSTT
                     SNSISWDYEYGIGCSGNNNNTGKYTNNTTGVLPATLASSNYRRPVIGFNYSLPPCTVP
                     PTAGIAISNIDTACSGEDFILNLSGNSFGDGQTYQWQYSTTGTAPWTDIGTADIEQRD
                     TLNQLTTTYYRCAVTCSGNTAYSDSVKIFTPTLVSGTFTIDQTLPPSGSNFTSFADAI
                     NSIKCGISGPVVFNVVAGSGPYTITSPISIPDVYGASPSNTITINGNNQSIVYNASVS
                     ADRSAIILNGADFVSLNNLSIDVSAGTYGWGITLINAADSNYIGSCNITSSTSSTSTN
                     YAGIVITGSLTSATSIGNNGNGNLIENNNIIGGYYGISTYGESTTLCAGNKLVNNSIQ
                     DFNYYGIQAAYQADAEIKNNDIQRPNRTTFSTTNYGMNFISVSTGLNVSGNKIHHLFS
                     ALPVNTITTYAIYVSADGTVTNPNKFYNNLIYSIEGDGTHYGVFNSGGAYMQAYHNTI
                     ALDYVAATAGTTAGFWQTTTADGIEFKNNIVYITRGGTGAKYCIYKATPATPLVSNNN
                     AFYLGSAGSGAQSIGYETSARASLSDWQTATSQDLNSLVADPVFTDPISGDYSFTSSI
                     IDNLGSPLGITTDIIGNSRSGSTPDVGAYEYVAPACIAPPTAGSATSSSPNVCNGETF
                     SLNLTGNSSGTGQTYQWQYSTTGSAPWTDIDIADGSPLKSVSQITSTYYRCAVTCSGN
                     TTYSTSVQVVSPAMVSGTFTINKLLPTSGTNFASFAEAIDSISCGINGPVIFNVVSGS
                     GPYALPSQIIINSIYGTSTSNTITINGNGETVTYNASVSETRTALLLNGIDHLIIDSL
                     TIDVSAGTYGYGIQFMNQSDSNIVRKSTILNNTTATTSNYAGIVYSGSLTSATIADDN
                     GNGNLLENNRIVGGYYGITNIGAASSLNSNNTIRNNSVEDFYYYGIYFSNQNNGIIEN
                     NNIHRPLRTNSSSCYGIYSTTGNKGLFINSNKIHNTFAGQVSSTSSLYGIYISSDAPA
                     ASPNLIANNLIYANEGNGTHYGLYSIGSDTALFYHNTVSLDYTPATAGATYGIYMSST
                     ASSGVQYKNNIISITRGGSGVKYGFYDLSSNLYASNYNNFYLNSLGAGAQYVGYKSST
                     AQATLLDWQTASSNDMNSYVLNPMFYNPSPIGSIYLPTNGSLQNSGTPLSIPTDINGD
                     TRNLSTPDIGAYEMPAFYHYRSQATGNWGDLSTWESSTDSSSWAAATTIPSYYSLSAV
                     VSNGHTVTINSSATIDKTFVNPTGILAVNDALTVYLNGLTFRSSSSGTGALGQSTGSI
                     TGNVVVERFIPAKATRKW"
BASE COUNT         2169 a         1020 c         1086 g         2240 t
ORIGIN      
        1 acagcttaat ggtacttttt cacttggttt tataatcata caattaccta aagctattgc
       61 attaggaata gtccaatttg gaaccataga aggaaaatta aaaggcacaa tagaggcaac
      121 aacgcctaat ggtgcatgtt ctgttctaca ctctacacct ttactaacct ctaaaatttc
      181 tccttgaatt aattgaggta atgaagtggc aaactctgta agctctacac atttttcaat
      241 ttctgcaaca gattcagcat aggttttgcc attttcttct gtacatagtt ctgctaattc
      301 ttttaaatgt ttttctaata aagttttata tctaaaaaat acttgaactc tttcttttat
      361 aggtgttttg ctccatgcag gaaatgcagc tttagctgca cgaacagcat tatctaaatc
      421 gttgtaagcc gacattggaa cagttgataa atgactacca tttaaaggag aaattacatc
      481 cattgtttta gttgtagatg cacttacaaa ttttccattg atgaaatttt gaatagcagg
      541 gtatttcatt gtttgttttt tattttactt tttaatttaa tcatagattt tctaaagaat
      601 atttttataa attttttaga aaatgagcta taaaactaaa attattttag aaataacaaa
      661 atatttttac tatttttatt ttaatggtaa ttatttattt ttaaaaaatt atgagtcaac
      721 attcattgga cacccttgat tttaatattc tgtcttgctt acaacaagat ggtagaatga
      781 gttttactgt aatggcagaa aaattaaatg tatctattgc aacagtaaga acgagagtaa
      841 ataaattaat agaagatgga actattaata ttatcggtag ggtaaatcca gaaaaagttg
      901 gctttcatgc gtatgcccat atcgcagtgt atgtacgccc tgcaacatta aaagaaaaag
      961 tagctaataa aatttcgaaa tataaagaag taagcttttt agcactaacc agtggagatt
     1021 atgatttaga ggttgatgtg atgtgtaaga ataatgattt tttggttgaa tttgtaaata
     1081 aaatttctac tatagaaggt gttcatcaaa ccaaaacaac catttatttt aaagtatata
     1141 aatatgcaca acccgatttg gatttattga gataaattta atggtgctga tttttttttg
     1201 actatataaa aaattatttt taccttctca ctctaaaact ttgctatatg ataaaattta
     1261 atgaattaca aacaggagat tttgtgatgg ttgagtatga aggtaaaatg tgggaaggag
     1321 aagtaaccaa ccttaaccac gatgaaaaac aagtagaagt aaccaccgag gtgcaacctt
     1381 tttggtacgc accagaaagt ttacatgctg taccattaaa cgaccaacag ttaatgaatt
     1441 ttaattttac caagcaagtt aatgaggatg gaagtattaa atatatgaaa gaagctttta
     1501 ggttagtgat accttcaaaa gatgatttta gcacaataga aatgtggtat agagaagata
     1561 gaagacatca tccagatgta cattttatgc accaactaca aaatcattat tacagcatga
     1621 ctaaagtaca tttaactaaa agttagttta ttttctggtt tcttctttta agtaataaac
     1681 tgttttagta gtatgtaatt tatattacat atacagtttt ctgttaagca gcaacttaaa
     1741 agaagatagt tagcctaatt tttttaactg ttagtataga aacctttgtt tacctcaaca
     1801 attgatattt ttttttaaaa aaaaaaaaat atactttagt agcccaattt aagaatggtt
     1861 tagctgctta tgaaactaca atatcttaat aagatatttt ttgcttataa tgaaatggaa
     1921 tggacaaaac actattgcca gttcattaag tctatttctc aacatagctt ttataatatt
     1981 tattttttat ttttcactaa aagaaaccat aaagtttttt ttaaagatta tttaaaagaa
     2041 tggatatgta aacccaaacc accttttcta catattacag taaattaaat tgtacaacta
     2101 atatttggtt cacctttttt ttaattgttt tttattcaat aataataaag tcatgaaaaa
     2161 atttttattc tttttttgtt ttctattact tgcaataggt agtactatag cacaacctat
     2221 gtggtcaatt gcaacttgta gtaatttaac ttcaactact tatgggccta tgtatagcac
     2281 ttctacagca aatgcaacta atagaacggc tattatttac cctgctacac aacttgtagg
     2341 ggtggcaaac acaacgctta cttctatgtt tattaaaaga gctactgcta gtggttcaat
     2401 gcttggtacg cccaatttta aaatttattt gttagaaact tcttctacag aatggggagc
     2461 tggaagttta gactgggcaa cagcaacagg accagccaca ttggtatatg atggcaaccc
     2521 agtttctgct gttggttctt ctgcagggtt taaagagttt tcactaacaa ccacgtttgc
     2581 ttatggaacc caaaatttag cggtgttttt tgaatatact aattctacca ctagtaatag
     2641 tataagttgg gattatgaat atggtattgg ttgcagtggc aacaataata ataccggtaa
     2701 atacaccaac aatacaacag gtgttttgcc tgcaacatta gcaagtagta attatagaag
     2761 gccagttatt ggctttaatt atagcttacc gccatgtaca gttccaccaa ctgcaggtat
     2821 tgcaataagt aatattgata cagcatgtag tggagaggat tttattttaa acctttctgg
     2881 aaatagtttt ggtgatggcc aaacctacca atggcaatat tctacaactg gtactgcacc
     2941 ttggacagat attggaacag ccgatattga acaaagagat actcttaacc aattaactac
     3001 aacttattat cgttgtgctg taacttgtag cggtaatacg gcttattcag attctgtaaa
     3061 gatatttact ccaactttgg ttagtggtac ctttactata gaccaaaccc tgccaccaag
     3121 tggaagtaat tttacaagct ttgcagatgc tattaattct ataaaatgtg gtattagtgg
     3181 tcctgttgta tttaatgttg tagcaggtag tggtccatac acaattactt caccaatttc
     3241 aatacccgat gtttatggtg catctccatc taacaccatc actattaatg gtaataacca
     3301 atcaatcgtt tacaatgcaa gtgtttctgc cgatagatca gcaataattt taaatggagc
     3361 cgattttgtt agccttaata atttaagtat tgatgtaagt gcaggcactt atggttgggg
     3421 tattacttta ataaatgcag cagatagtaa ttatattggc tcatgtaata ttacaagctc
     3481 tacttcttct acttctacaa actatgcagg tatagtaata acaggctcct taaccagtgc
     3541 cacatcaatt ggtaacaatg gtaatggaaa tttgatagaa aataataata tcattggtgg
     3601 gtattatggt atttcaactt atggcgaatc aacaacactt tgtgctggta ataaattagt
     3661 aaacaattct atacaagatt ttaattatta cggtattcag gctgcttatc aagcagatgc
     3721 tgagattaaa aataatgata ttcaaaggcc taacagaaca actttttcta caactaatta
     3781 tggaatgaat tttatatctg taagtactgg gttgaatgtt agtggcaata aaatacatca
     3841 cttattttcc gctttacctg taaatactat cactacctat gctatctatg taagtgctga
     3901 tggcactgta accaacccaa ataaatttta taataactta atttatagta tagaagggga
     3961 tggcactcat tatggagttt ttaattcagg aggtgcctat atgcaagcat atcacaatac
     4021 tattgcatta gattatgtag ctgctactgc aggtactact gccggttttt ggcaaacaac
     4081 tactgcagat gggatagagt ttaaaaataa tattgtgtat ataacccgtg gtggtactgg
     4141 agcaaaatat tgtatctata aagccacacc agctacacct ttagttagta ataataatgc
     4201 cttttactta ggcagtgcag gtagtggagc acaaagtata ggttatgaaa cttctgcaag
     4261 agccagttta tcagattggc aaacagctac aagtcaagac ttaaatagct tagttgcaga
     4321 cccagtattt accgacccaa ttagtggaga ttattctttt acttcttcta ttatagataa
     4381 tttaggtagc cccttaggaa taactactga tattattgga aatagcagaa gtggaagtac
     4441 tccagatgta ggtgcttatg aatatgtggc accagcttgt atagccccac caacagcagg
     4501 tagtgctacc agctcttctc caaatgtttg taatggtgaa acattttctc ttaatttaac
     4561 tggtaatagt tctggtactg gtcaaaccta tcaatggcaa tattcaacta caggatctgc
     4621 accttggaca gatattgata ttgctgatgg atctccacta aaatctgtaa gtcaaattac
     4681 ttctacttat taccgttgtg cagttacatg tagtggtaat actacttact ctacttcagt
     4741 gcaagtggtt tctccagcta tggtaagcgg tacttttact atcaataaat tattaccaac
     4801 tagtggtacc aattttgctt cttttgccga agctattgac tctataagct gtggtataaa
     4861 cgggcctgtt attttcaatg tagttagtgg ctctggtcct tacgctttac cttctcagat
     4921 tattataaat agtatttatg gtacttctac ttccaacaca ataactatta atggtaatgg
     4981 cgaaactgta acctataatg caagtgtttc tgaaactaga acagcactac tattaaatgg
     5041 aatagatcat ttaattatag atagtttaac aatagatgta agtgcaggta cttatggtta
     5101 tggtattcaa tttatgaacc aatcagatag caatattgtt agaaaaagta ctattttgaa
     5161 caacacaaca gctactacct ctaattatgc aggtattgta tatagcggtt cgttaactag
     5221 tgcaacaatt gcagatgata atggaaatgg taatttgtta gaaaataaca gaatagtagg
     5281 agggtattat ggcattacaa atattggtgc agctagttct ctaaattcta ataatacaat
     5341 tagaaataat agtgtggaag atttttatta ttacggaata tatttttcta accaaaataa
     5401 tggcatcata gaaaataata atattcaccg tccacttaga acaaattcta gttcctgtta
     5461 tggtatttat tctacaactg gtaataaggg attgtttatt aattctaata aaattcataa
     5521 cacttttgca ggtcaggttt caagtacttc ttccttgtat ggcatttata tttcttcaga
     5581 tgctcctgct gcaagtccaa atcttatagc caataacctt atttatgcaa atgaaggtaa
     5641 tggtacccat tatggtttat attctattgg ttcagatact gctttgttct atcataatac
     5701 tgtttcttta gattataccc ctgcaacagc tggtgcaact tatggaattt atatgagttc
     5761 tactgcatct tctggagttc agtataaaaa caatattata tcaataacaa gaggtggttc
     5821 tggtgttaaa tatggtttct atgacttatc aagcaattta tatgcaagca attataataa
     5881 tttctattta aatagtttag gagctggagc acaatatgta ggttataaat cttcaacagc
     5941 tcaggctact ttattagact ggcaaacagc atcttcaaat gatatgaaca gttatgtttt
     6001 aaatcctatg ttctataatc cttcacccat tggcagtatt tatttaccta ccaatggtag
     6061 cctgcaaaat tcaggaactc ctttaagtat tccaacggat attaatggtg acacaagaaa
     6121 tctttcaact ccggatattg gagcttatga gatgcctgct ttttatcatt atcgtagtca
     6181 agcaacaggc aattggggag atttatcaac ttgggagtct tcaacagata gttcttcatg
     6241 ggctgctgct acaacaatac ctagctatta ttcattaagt gcagttgttt ctaatgggca
     6301 tacagttaca attaatagtt ctgcaacaat tgataaaaca tttgtaaatc caacaggaat
     6361 attggctgta aacgatgctc taactgttta tttaaatgga ttaacattta gatctagttc
     6421 ttctggaaca ggagcattag gtcagtcaac aggctctatc accggtaatg tagttgtaga
     6481 acgctttata ccagcaaaag ctaccagaaa atgga
//
LOCUS       DAOQPK010000117         6498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_6825_length_6498_cov_4.391588, whole genome shotgun sequence.
ACCESSION   DAOQPK010000117 DAOQPK010000000
VERSION     DAOQPK010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6498
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6825_length_6498_cov_4.391588"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PLU36_06580"
     CDS             complement(<1..616)
                     /locus_tag="PLU36_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HMZ46453.1"
                     /translation="MKVHFISIGGSVMHQLAIALKHKNYTVTGSDDEIFEPAKTNLRN
                     EGLLPLKEGWQPELITNNLDAIILGMHAKEDNPELLKAKELGLKMYSFPEYIFQESLN
                     KKRIVVGGSHGKTTTTSMLMHVLKNVETEFDFLAGAILKDFKQSVKITNAPLIVCEGD
                     EYPASVLEKRPKFHFLFPHIAILTGIAWDHINVFPTFEIYKEQFL"
     gene            765..893
                     /locus_tag="PLU36_06585"
     CDS             765..893
                     /locus_tag="PLU36_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46454.1"
                     /translation="MKKFLVAVVFLSFTAILLSSCGAARYGAGKGCPTTNPRYFRG"
     gene            921..1247
                     /gene="ytxJ"
                     /locus_tag="PLU36_06590"
     CDS             921..1247
                     /gene="ytxJ"
                     /locus_tag="PLU36_06590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HMZ46455.1"
                     /translation="MQFFSLNSTTALEALRQNSFQQTQIIFKHSTRCSISKMVFNRFS
                     NVTNFANGNYHLLLVIEDRALSNTIADDFNIKHESPQILVIKNGICIYNESHTAINME
                     DIIAIA"
     gene            complement(1251..2285)
                     /gene="mltG"
                     /locus_tag="PLU36_06595"
     CDS             complement(1251..2285)
                     /gene="mltG"
                     /locus_tag="PLU36_06595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HMZ46456.1"
                     /translation="MKKIFLYLVLVSALLALIIGYFAFTSTTKFTSKSTTFIIDNINK
                     QQISNLLVEKNIISNKTVFLWIGNAVGIWEKIKPGKYEVKKGESIIGIIKMLKNGRLA
                     ESKLVINKLRVKEDFAKLVSKNFSTDSVTVMQFLTSTDSLKMYAVDTNTVFTLFIPDT
                     YAFYWNTPLRKIMNKLNEAKNNFWNNNNRLEKSKAIGLSPENIYILASIVEEETNYKE
                     DRAKIASVYINRLHKNMPLQACPTIKYAMKDFALTRIYEKYLVNPSPYNTYRVKGLPP
                     GPICTPSPKCIDLVLNAPQTDYLFFVANANFDGYHHFSTNYAEHNKYAIEYQKALDLY
                     TAKKKEKNSK"
     gene            complement(2334..3107)
                     /locus_tag="PLU36_06600"
     CDS             complement(2334..3107)
                     /locus_tag="PLU36_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ46457.1"
                     /translation="MIEIKNITKSFSGRTIISNINATFETGKCNLIIGASGSGKSVLL
                     KVMVNLFTPDNGEALYDGISITALEQERRKELRQQIGMLFQGSALFDSMTVEQNVLFP
                     LDMFTKLSQADKKKRVNEVLERVNLVDANKKYPSEISGGMKKRVGIARAIVLNPKYLF
                     CDEPNSGLDPQTSLLIDKLIKEITTEYNMTTIVVTHDMNSVMEIGDYIMYLHKGSKQW
                     EGSNKDIIYSNDELLNKFIFASEFLQDAKQMRMLEENNK"
     gene            3249..3833
                     /locus_tag="PLU36_06605"
     CDS             3249..3833
                     /locus_tag="PLU36_06605"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HMZ46458.1"
                     /translation="MSNKLEILSEIIANRRTTKPQLMNGKLIEEKTILKLLELANWAP
                     THANTEPWRFIVYTPETKTKFCKAHAALYKLHTDKDKFLLATFQKLEQMGNLASHIII
                     AYAKSGSNIKIPIVEEVAATSAAIQNFILAAEALNIATYWGTGGMIHQPVFKSFLNLN
                     TDDVVLGTIYLGYTNEKKEGKRITTIHEKINWVR"
     gene            complement(3853..4431)
                     /locus_tag="PLU36_06610"
     CDS             complement(3853..4431)
                     /locus_tag="PLU36_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnA domain-containing protein"
                     /protein_id="HMZ46459.1"
                     /translation="MSLEKNLQTRSNNECELCTAKKSLSIYNLPPDAEQLVEKSILIC
                     PTCLSQVEKKAPLDKEHWQCLLTSMWSEVDAVKVVSWRMLNRLREETWASDALEMLYF
                     EDDILNWAKATGDHENSSDIEFHTDCNGAILQNGDTVVLIKSLDVKGSTVNARLGTVV
                     KNIRLVADNTSQIEGKIEGQTIVILTKYLRKG"
     gene            complement(4473..4925)
                     /locus_tag="PLU36_06615"
     CDS             complement(4473..4925)
                     /locus_tag="PLU36_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HMZ46460.1"
                     /translation="MQNIFNLTDTNELIKRINNLTPNTQPQWGKMNVVQMLAHCNVTY
                     EMIFENNFPKPTGIKKLILKYIIKNIVVGEKPYKKGIPTAPQFIITDSREFEVEKNRL
                     IHYINKVQQLGENFFDGKESHSFGKLTKQQWNNMLGKHLNHHLAQFGV"
     gene            complement(5022..>6498)
                     /locus_tag="PLU36_06620"
     CDS             complement(5022..>6498)
                     /locus_tag="PLU36_06620"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ46461.1"
                     /translation="LDSDNDGIPDIVEVGALDNNNNGKVDTFIDTNFNGINDSLEGVS
                     NALVLSGSDTNNNGVADSWPNKNADRTGRPNLYDLDSDGDGITDAEESGLIGRTGTNG
                     TVASSAGIITGTYSNGWANTVQGLASLNLPNTDARGSANYLDIDSDDDGITDNVEAQS
                     TSSYVVPTDTDSDNDGIADVYEVPAQIGTYGGNGLTPYNKDGDGNPDYKDTDTDNDGA
                     LDYIEGTGSSLYGVAYNTLNFTDTDGDGLVDQWDIANISSLTAGNLYRNVTHSDMGPG
                     GNLDGPAPSGSSARLPKHTWGPSTDRDWRSNIILPLNIISFKVNYNAPNAVINWEVQN
                     ELQTENYDVEFSINGTEFTKIFTVQAKNIGNANYTQWHNLDNYTQNVFYYRIKQTDKD
                     GKIYYTNIASIKVNRDSKITVAPNPFRNHFSVNYNSVSNEKVTISLISNDGKIISTKQ
                     VDVVKGINQIMFDNLQYLQHGLYFLRVQTLKNITTLKLLKE"
BASE COUNT         2094 a         1093 c          894 g         2417 t
ORIGIN      
        1 tgaggaactg ttctttatat atttcaaatg ttggaaaaac attaatatga tcccatgcaa
       61 taccagttaa aatagctata tgaggaaata aaaaatgaaa tttaggtctt ttttctaaaa
      121 cacttgccgg atactcatct ccttcgcaaa ctattaaagg tgcgtttgta atttttactg
      181 attgtttaaa gtctttaagt attgccccag ccaaaaaatc gaattcggtt tcaacatttt
      241 taagtacatg catcagcata cttgtggtgg ttgttttgcc atggctacca cctacaacta
      301 ttcgtttttt atttaggctt tcctgaaaaa tatactccgg aaaagaatac atttttaagc
      361 ctaattcttt tgccttcaac agttcagggt tatcttcttt ggcgtgcata cctaaaataa
      421 ttgcatctaa attattggta attaattcgg gttgccaacc ttcttttagg ggtaataatc
      481 cttcgtttct taaattcgtt tttgcaggtt caaaaatttc atcatcggaa ccggtaacgg
      541 tataattttt atgttttaaa gcaattgcta attggtgcat aacactacca ccaatagaaa
      601 taaaatgaac tttcataaat taaacgtgta attaatttgg atttatttat agctgaaaat
      661 gactaaaatg agatattttt ttcactaaaa tgcaacattt ataatgaaaa tactatcttc
      721 gtaataataa acaaattaaa aaatttattt tttaaatatc ttctatgaag aagtttttag
      781 tagccgttgt ttttctttca tttactgcaa tattattatc atcttgtggt gctgcacgtt
      841 atggtgcagg aaaaggttgt cctacaacta acccaagata ttttagaggc taatttttgc
      901 caataatatt ataggtattt atgcaatttt tttcgttaaa ttctaccact gctttagaag
      961 cattgaggca aaactccttt cagcaaactc aaattatttt taagcatagt acacgttgct
     1021 ccattagtaa aatggtattt aacaggttta gtaatgttac caattttgct aatggtaatt
     1081 atcatttatt attggttatt gaagacagag ctttatctaa caccattgca gatgatttta
     1141 atattaaaca tgaatctcct caaattcttg ttataaaaaa tggtatatgt atttataacg
     1201 aaagccatac tgctataaat atggaagata ttattgcaat tgcttaaaac ttatttagaa
     1261 tttttttctt tcttttttgc agtgtataaa tctaacgcct tttggtattc aattgcatat
     1321 ttattatgct cggcatagtt agtagaaaag tgatgataac catcgaaatt tgcattggct
     1381 acaaaaaata aataatctgt ttgaggggca tttaaaacta aatcaataca cttaggagaa
     1441 ggagtacaaa ttgggcctgg aggtaaacct tttaccctat aagtgttata cggagaaggg
     1501 tttaccaaat atttttcata aatacgagtt aaagcaaaat ctttcatagc atatttgata
     1561 gtaggacagg cttgcaatgg catattttta tgtagcctgt ttatatatac gctagcaatt
     1621 ttggctctat cttctttata atttgtttct tcttctacta tagatgctaa tatataaatg
     1681 ttttctggcg ataagccaat tgctttagat ttttctaatc ggttattatt attccagaaa
     1741 ttatttttag cttcattaag tttattcatt atttttctaa gtggcgtatt ccaataaaac
     1801 gcatacgtat ctggtataaa taaggtaaaa actgtattgg tatccactgc atacattttt
     1861 aaagaatcgg ttgaggttaa aaactgcatg acagttacag aatcggtaga aaaatttttg
     1921 cttactagtt ttgcaaaatc ttcttttact ctaagtttat tgattactaa tttactttcg
     1981 gccaatcttc catttttaag cattttaata atgccaataa tactttcgcc ttttttcacc
     2041 tcgtattttc ctggcttaat cttttcccaa attcctacag catttccaat ccataaaaac
     2101 acagttttat tgctaataat atttttctca acaagtaaat tacttatttg ttgtttgtta
     2161 atattatcaa ttataaatgt tgtcgattta ctagtaaact ttgtagtaga agtaaaagca
     2221 aaatagccaa taattaaggc aagtagagca ctaactagaa ccaagtacaa aaatattttt
     2281 ttcattttaa taaaaaatgt ttagaaaatt attgccaaga ataattgtaa taattattta
     2341 ttattttctt caagcatacg catttgttta gcatcttgta aaaactccga agcaaaaata
     2401 aatttattca ataattcatc attgctgtaa ataatatctt tattgcttcc ctcccattgc
     2461 ttactacctt tgtgtaagta cataatgtaa tctccaattt ccatcacact attcatatca
     2521 tgtgtaacaa caatggtagt catattatat tcggtagtaa tttcttttat taatttatca
     2581 attaataaag atgtttgagg gtctaaacca ctattaggtt catcgcaaaa aagatattta
     2641 gggtttaaca caattgctct agcaatacct actctttttt tcattcctcc gctaatctca
     2701 gaaggatatt ttttatttgc atccactaaa ttaactcttt ctaaaacttc atttacccgt
     2761 ttctttttat cggcttgaga tagtttagta aacatatcta gaggaaataa aacattttgt
     2821 tcaaccgtca tactatcaaa taaagcactg ccttgaaaca gcattcctat ttgctgtctt
     2881 agttctttcc ttctttcttg ctctaatgca gtaatgctta taccatcgta taatgcctct
     2941 ccattatctg gtgtaaaaag attaaccatt acttttagta gtacactttt accactacca
     3001 ctagccccaa taattaaatt gcattttccg gtttcaaaag ttgcattgat attgctaata
     3061 atggttctgc ctgaaaatga tttggtaata tttttaattt ctatcataac aaatgtttgt
     3121 aaagttatgc taatgcaagc aaattattgg tgtacaatat ttctattata gttgcttatt
     3181 gattaaattt taacaaccaa tgcatgtatt tttgaggtgt aaacaattta attaaataca
     3241 attgaataat gagcaacaaa ttggaaattt taagtgaaat tattgctaac agaagaacca
     3301 ctaaacctca attaatgaat ggcaaattaa ttgaagaaaa aaccatttta aaattactgg
     3361 aacttgccaa ctgggctcct acacatgcta atacagaacc ttggaggttt attgtttata
     3421 cgccagaaac taaaacaaag ttttgtaaag cacatgcagc attatataaa ttacacacgg
     3481 ataaagacaa atttttgcta gcaacttttc aaaaacttga acaaatgggc aaccttgcct
     3541 ctcacattat tattgcctat gccaaaagtg gtagcaatat taaaatacca attgtagaag
     3601 aggttgcagc cactagtgca gctatacaaa attttatact agctgcagaa gctttaaata
     3661 ttgctaccta ttggggcact gggggcatga tacaccaacc agtttttaaa agcttcttaa
     3721 atttaaatac agacgatgtt gttttaggta ctatttattt gggttatacc aacgaaaaaa
     3781 aagaaggaaa aaggattact accattcatg aaaaaataaa ctgggtacga taaagtttaa
     3841 ggaatacaaa gtttagcctt tgcgtaaata cttggttaat atcacaattg tttgtccttc
     3901 tattttacct tcaatttggc tggtattatc tgctacaagc ctaatatttt ttacaacagt
     3961 tcctaacctt gcatttactg tggagccttt tacatctaat gattttatta aaactacagt
     4021 atcaccgttt tgcaaaatag caccattgca atctgtatga aactcaatgt cagaggagtt
     4081 ttcatggtct ccagttgctt tagcccaatt taaaatatcg tcttcaaaat acaacatctc
     4141 taatgcatca gaagcccaag tctcttctct caatctgttt aacattctcc acgataccac
     4201 tttaactgca tctacttcgc tccacatgct tgttaataaa cattgccagt gttctttatc
     4261 aagaggtgct tttttctcta cttgacttaa gcatgtaggg caaattaaaa tgcttttttc
     4321 aactaattgt tcagcatctg gcggtaaatt atagatagat aaactttttt tagctgtaca
     4381 taactcacat tcattgttac ttctggtttg taggtttttt tctaaactca ttatctctta
     4441 ttttaaagtt tgcaaaataa aaattaaacc ttttatacac caaactgtgc taaatggtgg
     4501 tttaagtgtt tgcccagcat gttattccat tgttgcttag ttaatttacc aaaggagtgt
     4561 gattcttttc cgtcaaaaaa attttcgcct aattgttgta ctttattaat gtaatgtatc
     4621 aatctatttt tttcaacctc aaattctcta ctatctgtta ttataaattg cggtgctgtt
     4681 ggaattcctt ttttataagg cttttctccc actacaatat tttttattat gtattttaaa
     4741 attaattttt taattccggt tggtttaggg aaattatttt caaaaatcat ttcataagta
     4801 acattgcaat gtgctaacat ttgtaccaca ttcattttac cccattgtgg ctgtgtattt
     4861 ggtgttaaat tatttattcg ttttattaac tcgttggtat ctgtaaggtt gaaaatattc
     4921 tgcatttgtt aactctttta aaaaaatttt ggcgaaagta aataaaatag cctcattaaa
     4981 aatttaaaat gaggctaaat tgaaatttta tgtacttaca actattcttt tagaagttta
     5041 agtgtagtaa tattttttaa agtttgaact ctcaggaaat ataaaccatg ttgtaaatat
     5101 tgtaagttat caaacataat ttgattaatg cctttaacaa catctacttg ttttgttgaa
     5161 ataattttac catcattact tattaaactg attgtaactt tctcgttgct tacactatta
     5221 taatttacac taaaatggtt tctaaatgga ttaggagcaa ctgtaatttt gctatctcta
     5281 ttcactttta tagaagcaat gttagtataa tatatttttc catctttatc cgtttgttta
     5341 attctataat aaaaaacatt ttgagtgtaa ttatctaaat tatgccattg ggtgtaatta
     5401 gcattgccta tgttttttgc ctgcactgta aatattttgg taaactctgt accatttatg
     5461 ctaaattcaa catcataatt ttcagtttgt agctcgttct gaacttccca attaataaca
     5521 gcattaggag cattatagtt taccttaaat gaaatgatat ttaagggaag aataatgttg
     5581 ctacgccaat ctctatctgt agatggaccc catgtatgtt tcggtaatct tgcagatgaa
     5641 ccagaaggag ctggaccatc cagattacca cctggaccca tatcgctatg tgttacattt
     5701 ctgtaaagat tacctgctgt taatgaactg atatttgcaa tatcccattg atccactaac
     5761 ccatcaccat cggtatctgt aaaatttaaa gtgttgtatg caacaccata taatgatgat
     5821 cctgtacctt ctatataatc taaagcacca tcattatcag tatccgtatc tttataatct
     5881 gggttaccat ctccatcttt attataaggt gttagcccat tacctccata agttccaatt
     5941 tgagcaggta cctcgtaaac atctgcaata ccatcgttat cagaatcagt atctgtaggt
     6001 acaacataac tacttgtaga ttgagcctct acattgtcag ttattccatc gtcatcgcta
     6061 tcaatatcta aataatttgc agagccacga gcatcggtat tgggtaaatt tagtgaagct
     6121 aaaccttgca ctgtatttgc ccatccatta ctataagttc ctgtaattat acctgcactg
     6181 ctagcaacag taccattagt acctgttctt cctattaaac cagattcttc tgcatctgtt
     6241 attccatcgc catcactatc taaatcatac aaattaggtc ttccagttct atctgcattt
     6301 ttatttggcc aactatcggc aacaccatta ttattggtat ctgagccaga taacaccaaa
     6361 gcattagaca ctccttctaa actatcattt attccattaa aattcgtatc aataaaagta
     6421 tctaccttgc cattattgtt attatccaaa gctcctactt caacaatatc tggaatacca
     6481 tcattatcgc tatctaaa
//
LOCUS       DAOQPK010000118         6496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_6828_length_6496_cov_4.403509, whole genome shotgun sequence.
ACCESSION   DAOQPK010000118 DAOQPK010000000
VERSION     DAOQPK010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6828_length_6496_cov_4.403509"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4541)
                     /locus_tag="PLU36_06625"
     CDS             complement(<1..4541)
                     /locus_tag="PLU36_06625"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46462.1"
                     /translation="MRKIILLMFIFLSGTFLAQSQTNVQIGTGTGTSTYFPIYYLYDY
                     SYSQTIYSAAELTSGGATSSGMISKIFFKSTAAASTVGKWKNWTIYMANTAQQGFTGT
                     SNYIPASTMTQVFNGDLPDNVSANVWIELTLSTPFLWDGSSHLAITVMDNTLGWGNAP
                     TWSGYTATPISGALTRGIYFYQDGTTINPNSPSAVSSGTTNQIAQVQLEWTPASACSG
                     TPTAGTAEAPLTVQCPNTSFGLALNGTTSASGLTYQWQSRPTSGGSWADIFGATTKSY
                     TLTSGVSVNTDFRAYVTCTNGNLTDTSTVTSVTVSTLAAAGTYTINSSLPTSGSNFAN
                     FEDAYDFIKCGIAGPIVFNVAVGSGPYTITSPLKLPKINGTSATNTITFNGNGESILY
                     NTSDANNRAAIVLNGADNIIIDSFTVDVSAGTYGWGIVLTNRADSNTIRKCTILTSTT
                     ATTTNYAGILITGSVTGPTTAGENGNDNLIENNRIVGGYYAITAIGTTTTLDSNNIIK
                     NNLIEEYYYYGIYTSYEHNLQIIGNDFSRPTRTNPSTFGYSIYSTTGNVGVLIDKNKI
                     HNLFTGLPVNTSTNYAIAVISAGTATYPHIISNNLIYNIEGNGTHYGIYNSSAAYTKI
                     YHNTIALDYIGATAGITYGFYQTGNVNGIEFKNNIVFITRGGSGVKYAIYKSTAATPI
                     ASNNNVFFLGSAGSGTQNIGYQTSASADLITWQTTSGQDALSKAADPLFLNPSSGDYS
                     TTEPTIDDMGAAVGVLKDINGINRSLTTPDPGAYEKPPSVGIDMKPVMMLTPITSTPG
                     CYNTETLAVQIENNSVDSINFSINPVTVTINVTGAATATYTAIVNTGKLAPDSSLNVT
                     MTTPGATLNMSTAGAYNFDISTSVTGDVNSANNLISTTRTKISLTAGTIASSQTELCA
                     SGTPPLFTLTNAEGYSSIKWQVSNTSGSGFTDIPGANSLTYTLGAVPISTKYFRVVAT
                     CGSTDVNSPELTITVVNPQITSTTPGSRCGPGTVNLSALATGFDVRWYADSVGGAPLY
                     TGNAFTTPIINATDTFYAAAINNPTPVPPVVVGAGGTTSSSYESPYYYLYGNKLSQYL
                     FRASELTALGMTAGSITSVAFDVVSAASSYSNFSISFKSTNATDMSLTLESGLTTVYT
                     SAMETPTAGVNTYSFSSTPFAWDGASNIIMQICWGNSTSGGTSATVKMDATSFAANAY
                     YRVDGAMPVTFCNTTAANGTTNSRPQTTFGFDASCVGSRVPVIATINTPPAIVASVIN
                     DSICSGQSTTISVSSSNSGYTYTWDPGSLIGASHSVAPLTKTKYIVNANDASGGMYNG
                     CTNKDSVEIVVKPVPTAVTPSSTPGTICTSNGEIAKLVANGGNIGGEFTFGTAAAQNI
                     SSGYPAPYTAYYGGQRMQMLITAAELTGQGVQAGLLNSISFPVVSLGANWGGSITSCI
                     DFQMSVGATSLTTLTAFQSGLTQVVAPGNYTPTVGNNNVHTFTTPFMWDGVSNLIIET
                     TFSNNFLGGTSDAVIQYNTT"
     gene            4681..5229
                     /locus_tag="PLU36_06630"
     CDS             4681..5229
                     /locus_tag="PLU36_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HMZ46463.1"
                     /translation="MANRRDFDIAFVGLKPGIHVFEYRLEDQFFLNYGNQDFSNCIAN
                     IKLHLDKHAGFMQLKFDIDGTVNVNCDRCGNTIQQQLWDEFNIIVKMTDEPEIMNEQE
                     EDPDIYYISRGESHIHISDWLYEFVSLSVPMQNICGNDENGKSLCNQEVITKLEQMQA
                     KNINAENQMWKGLEQFKDLEKE"
     gene            5318..5512
                     /gene="rpmF"
                     /locus_tag="PLU36_06635"
     CDS             5318..5512
                     /gene="rpmF"
                     /locus_tag="PLU36_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HMZ46464.1"
                     /translation="MPNPKRRHSQQRSAKRRTHYIAESVTLSKDTATGETHVRHRAHV
                     SEGKLYYKGKLVAEKAPLKA"
     gene            5625..>6496
                     /gene="plsX"
                     /locus_tag="PLU36_06640"
     CDS             5625..>6496
                     /gene="plsX"
                     /locus_tag="PLU36_06640"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HMZ46465.1"
                     /translation="MNIGFDLMGGDFAPTEAVKGLKLYLQQANNVTVIGIANKAIIEP
                     LLAEHSIQNNIQLIHSDEVIGYNEHPTKALKEKPNSSIAIGFHLLATGKIDAFISAGN
                     TGAMLVGAMFSIKAIAGVQRPTIATIIPKTNGTTGLLLDVGLNADCKPEHLNQFATLG
                     NLYAQNILGIPNPTTGLLNVGEEEGKGNILAQATYPMLKENTMINFVGNVEGRDIFND
                     KADVIVCDGFTGNVVLKMAESFYDIATQRNLHNDTYLSRFHYENYGGTPVLGVSKPVI
                     IGHGISNDVAFKNM"
BASE COUNT         2192 a         1095 c         1155 g         2054 t
ORIGIN      
        1 gtagtattat actgaattac tgcatcacta gttccaccaa ggaaattatt agaaaaagtt
       61 gtttcaataa ttaaattgct aactccatcc cacataaatg gagtggtaaa tgtatgcaca
      121 ttattgtttc ctacagtagg agtataatta cctggagcaa ctacttgagt taatccagat
      181 tggaaagcag ttaaagtagt taaacttgtg gcaccaacac tcatttgaaa atcgatacaa
      241 ctagtaatac ttcctcccca attagcccct aaagaaacaa cagggaaaga aatactattt
      301 aacaatccag cttgaactcc ttgtcctgtt aactctgctg cagtaattaa catctgcatt
      361 ctttggccac catagtaagc agtataaggg gctggataac cagaagaaat gttttgtgct
      421 gctgcagttc caaaagtaaa ttctcctcct atattaccac cattagcaac taatttagca
      481 atttctccat ttgaagtaca aatagtacca ggtgtactac ttggggtaac agcagtaggt
      541 acaggtttta ctacaatttc aactgaatct ttgttagtac agccattata cataccacca
      601 cttgcgtcat ttgcatttac aatatatttt gttttagtta atggagcaac ggagtgagaa
      661 gcaccaatta aagaaccagg atcccaagta taagtataac cagaatttga actactaaca
      721 ctgatagttg tactttgtcc actacaaata gaatcgttaa taacagaagc cacaattgca
      781 ggtggagtat tgatggttgc aattacaggt acacggctac ccacacaaga agcatcaaaa
      841 ccaaatgttg tttgtggtct actatttgtt gttccatttg ctgcagtagt attacagaag
      901 gtaacaggca ttgcaccatc cactctataa taagcatttg ctgcaaaact tgttgcatcc
      961 attttaacag tagcactagt tcctccacta gtactattac cccaacaaat ttgcataatt
     1021 atgttagaag ccccatccca tgcaaatgga gtacttgaga agctgtatgt attaacacct
     1081 gctgtaggag tttccatagc agaagtataa acagttgtta ggccagattc taaagttaaa
     1141 gacatgtctg ttgcattggt ggacttaaat gaaatactaa aattactata actagatgca
     1201 gcagagacta catcaaatgc tacagaagtg atactgcctg ctgtcattcc caatgcagtt
     1261 aattctgaag ctctaaataa gtattggctt aatttatttc catataaata gtaatatggg
     1321 ctttcataac tggaacttgt agtgccacct gcacctacaa ctactggcgg tacaggagta
     1381 gggttattta ttgcagcagc ataaaaagta tctgttgcat ttattatagg cgttgtaaat
     1441 gcattacctg tataaagtgg ggcaccacct acagaatcgg cataccatct aacatcaaag
     1501 cctgtagcca atgcacttaa atttacagtt ccaggtccac agcggctacc tggtgttgta
     1561 gaagtaattt gaggattcac cactgttatt gtcaattcag gtgaattaac atcagtagaa
     1621 ccacaagttg ccaccactct aaagtatttg gttgatatag gaactgcacc aagtgtataa
     1681 gttagtgagt tagctcctgg tatatctgta aatccagagc cactagtatt actaacttgc
     1741 catttaatac tactgtaccc ttctgcatta gttaatgtaa ataaaggagg agtaccagaa
     1801 gcacataatt cggtttggct actagcaatt gtgcctgctg ttaaacttat ttttgttcta
     1861 gttgtgctaa ttaaattatt agcagaattt acatcgccag ttacagaggt agaaatatca
     1921 aaattataag ctcctgcagt actcatattt aatgtggcac caggtgtagt cattgttaca
     1981 tttaagctag aatctggtgc taattttcca gtattaacaa tagctgtata tgtagctgtt
     2041 gcagcaccag ttacattaat agtaactgta acaggattaa tactaaaatt gatagaatca
     2101 acactgttat tttcaatttg aactgcaagg gtttcggtgt tataacatcc tggagtagaa
     2161 gtaattggtg ttaacatcat cactggcttc atatcaatac caacagaagg tggtttttcg
     2221 tatgctcctg gatcaggtgt agttaagctc ctgtttatac cattaatatc ttttaaaaca
     2281 ccaactgcag cccccatatc atcaattgtt ggctcagttg tagaataatc accactacta
     2341 ggatttaaaa ataaagggtc agcagcctta cttaaagcat cctgaccact agtagtttgc
     2401 caagtaatta aatctgcaga agcactagtt tggtaaccta tattttgtgt accactaccg
     2461 gcacttccta aaaagaatac attattgtta cttgcaattg gtgtagctgc tgtagattta
     2521 taaatagcgt atttcactcc actacctcca cgggttataa acactatatt atttttaaat
     2581 tcaataccat taacattgcc tgtttgataa aaaccataag taatacctgc tgtggcacct
     2641 atataatcaa gagctatggt attatgataa attttagtgt aggctgcact gctattataa
     2701 attccatagt gtgttccatt accttctata ttatagatta ggttattgga aattatgtgt
     2761 gggtatgtag ctgttccagc ggaaattaca gcaattgcgt agttggtaga ggtatttaca
     2821 ggtagaccgg taaataaatt gtgtatttta tttttgtcaa ttaatacacc tacattacca
     2881 gttgtagagt aaatactata cccaaaagta ctcggattag tacgtgtagg tctagaaaaa
     2941 tcattaccta taatttgtaa attatgctca tagcttgtat aaataccata atagtaatat
     3001 tcttctatta aattattctt aataatattg ttagaatcta aagtagttgt ggtaccaata
     3061 gctgtaatag cataatatcc accaactatt ctattgtttt ctattagatt atcattacca
     3121 ttttcgccag ctgtagttgg accggtaaca gaaccagtaa ttaaaatacc tgcataatta
     3181 gttgttgttg cagttgtact tgttaaaatt gtacattttc taatagtatt actatccgct
     3241 ctgttcgtta atacaatacc ccatccataa gtacctgcac ttacatcaac cgtaaagcta
     3301 tcaattatta tattatcagc accattcaaa acaatggctg ctctattatt tgcatctgag
     3361 gtattgtata atatactttc tccattacca ttaaaggtaa tagtattggt tgctgaggtg
     3421 ccattaattt ttggtaattt taatggggag gtaatagtat atgggccact acctacagca
     3481 acattaaata caatagggcc agctatacca catttaataa aatcataagc atcttcaaaa
     3541 ttggcaaaat tgctaccact agtaggcaaa gatgagttaa ttgtatatgt tcctgctgct
     3601 gctaatgttg atacagttac tgaggtaaca gttgaagtat ccgttaaatt accattagta
     3661 catgttacat aagctctaaa gtctgtatta acactaactc cgcttgttaa cgtatagctt
     3721 tttgttgttg ctccaaatat atcagcccaa cttccaccgc ttgtaggcct tgactgccat
     3781 tgataagtaa ggccactggc agaagtggtt ccatttaagg ctaaaccaaa tgaagtatta
     3841 gggcattgta ctgttaatgg tgcttctgca gtacctgcag taggcgtacc agaacaagca
     3901 gatgccggcg tccactcaag ttgtacttga gcaatttggt ttgtggttcc tgagctaaca
     3961 gcactggggg aattagggtt tatagtcgtt ccatcttgat aaaaatatat acctcttgtt
     4021 aatgcacctg aaataggtgt tgcagtataa ccagaccaag tgggtgcatt accccatcca
     4081 agagtattat ccattacagt aattgccaag tggctcgacc catcccataa aaaaggtgtg
     4141 gataatgtaa gttctatcca aacatttgct gaaacattat cgggtaaatc tccattaaaa
     4201 acttgcgtca tagtgcttgc aggtatataa ttactagtac ccgtaaagcc ttgctgagca
     4261 gtgtttgcca tataaatagt ccagtttttc catttaccta ctgtacttgc tgcagcagtg
     4321 gacttaaaaa atattttaga aatcattcct gaagatgtag cacctcctga agttagttct
     4381 gcagcagaat aaattgtttg agagtagctg taatcgtaca agtaataaat tgggaaatat
     4441 gtactcgtac ccgttcctgt acctatttgc acattagttt gagattgtgc aagaaaagtt
     4501 ccagaaagaa aaataaacat gagtaaaata atctttctca taatcagtta gtttggttta
     4561 aaaatctaaa ataagtaatg tgagcaaaat aatcaaatag ttttaaaaaa caaagattat
     4621 caacaatctt ttgtaagttt aattcaaact actacctttg ccaaccctaa aaatttacac
     4681 atggctaatc gcagggattt tgatattgct tttgtagggt taaaaccagg cattcatgtg
     4741 tttgaatata ggttagagga tcagttcttt ttaaattatg gtaatcagga ttttagtaat
     4801 tgtattgcaa atattaaact tcaccttgat aaacatgctg gctttatgca attgaagttt
     4861 gatattgatg gaacagtaaa tgtaaattgt gacagatgtg gtaacaccat acaacaacaa
     4921 ttatgggatg agtttaatat tattgtaaaa atgactgatg agccagagat aatgaatgag
     4981 caggaagaag acccggatat ttattatata agcagaggtg aaagtcatat tcatatttcc
     5041 gattggttat atgagtttgt aagtttaagt gtgccaatgc aaaatatttg cggaaatgac
     5101 gaaaatggca aatcattatg taatcaagag gttattacta aacttgagca aatgcaagca
     5161 aagaatatta atgcagaaaa ccagatgtgg aaaggattag aacagtttaa agatttagaa
     5221 aaagaataag tacaaaataa tataaaagct actacaaata acttttatta tttaaaaaca
     5281 aataaaaaca attggattaa ttttaaaaat ttgagttatg ccaaatccaa aaagaagaca
     5341 ctcacaacaa cgtagtgcaa aaagaagaac acattatatt gctgaatcag ttactttgag
     5401 taaagatact gcaacaggag aaacacatgt acgccaccgt gctcatgtaa gtgaaggaaa
     5461 attgtattat aaaggaaaat tagttgcaga aaaagctcct ttaaaagctt agcaactact
     5521 atgttgaatt ctttaaataa tttaagtgtc aggtacttta gtggtatcag acacttgttg
     5581 ttttatagaa gttagtacaa tttaccttta aatctaatgg aagcatgaat attggttttg
     5641 atttaatggg tggtgatttt gcaccaaccg aagcagttaa agggcttaaa ctatacttgc
     5701 aacaagcaaa taatgttact gtaattggca ttgctaataa agccattatt gaacctctat
     5761 tagcagaaca cagtattcaa aacaatatac aattaataca ttcagatgaa gtaattgggt
     5821 acaatgaaca tcctacaaaa gctttaaaag aaaagcccaa tagctctatt gcaattggtt
     5881 ttcacttatt agcaaccggt aaaatagatg cttttattag tgctggcaat actggtgcaa
     5941 tgttagttgg tgctatgttt agcattaagg caattgcagg agttcaaaga ccaacaattg
     6001 caactattat tccaaaaaca aatggcacta caggtttatt attagatgtt ggtttaaatg
     6061 cagattgtaa gccagaacac ttaaatcaat ttgctacctt aggtaatctt tatgctcaaa
     6121 atattttagg cattcccaat cctacaacag gtttattaaa tgttggcgaa gaagaaggca
     6181 agggtaatat acttgcacaa gccacctacc ctatgcttaa agaaaataca atgattaatt
     6241 ttgtgggaaa tgtggaaggg agagatattt ttaatgataa agcagatgta atagtttgtg
     6301 atggttttac aggaaatgta gtattaaaaa tggccgaaag tttttatgat attgctacac
     6361 aaagaaactt acataacgac acttatttaa gtaggtttca ttatgaaaat tatggcggta
     6421 cacctgtatt gggtgtttca aaaccagtaa taattggcca tggtattagc aatgatgtag
     6481 cttttaaaaa tatgtt
//
LOCUS       DAOQPK010000119         6491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_6833_length_6491_cov_4.391703, whole genome shotgun sequence.
ACCESSION   DAOQPK010000119 DAOQPK010000000
VERSION     DAOQPK010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6491
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6833_length_6491_cov_4.391703"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1636
                     /gene="katG"
                     /locus_tag="PLU36_06645"
     CDS             <1..1636
                     /gene="katG"
                     /locus_tag="PLU36_06645"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HMZ46466.1"
                     /translation="KWLATSKEANSRYSGERDLENPLAAVQMGLIYVNPEGPDGNSDP
                     LAAAVDMRETFARMAMNDEETVALIAGGHTFGKTHGAGDVALVGPEPEAAPIEAQGLG
                     WLSKHGSGKAGDTITSGLEVTWTSTPTKWTNNFFWNLFGYEWELETSPAGAKQWVPKH
                     GMGANSVPDAHDPNKRHAPKMLTTDMALRLDPTYEKISRRFYENPDVFADAFAKAWFK
                     LTHRDMGPISRYLGPEIPKVEFIWQDIIPVNQHELISKDDIGALKELILQSGLSIGEM
                     VSTAWASASTFRISDKRGGANGARVKLEPQKNWVVNNPTQLTKVISTLEAIQSKFNQS
                     NKKVSIADLIVLAGGTAIEKAAKDAGYSVIVPFMPGRADATMEQTEIDSIAVLEPFAD
                     GFRNYLKSKSTIATEELLIDKAQLLNLNAPELTVLIGGMRMLNTNYDGSNHGVFTSTP
                     ERLTNDFFVNLLNINTVWKAIDNTEEIFEGTDRTTGTKKYTATRNDLIFGSNSELRAL
                     AEVYASEDAKEKFVNDFVTAWTKVMNLDRFDLKNKS"
     gene            1707..2423
                     /gene="mtgA"
                     /locus_tag="PLU36_06650"
     CDS             1707..2423
                     /gene="mtgA"
                     /locus_tag="PLU36_06650"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HMZ46467.1"
                     /translation="MLLLQKLKLKNVWKWIKRVVIILFISSLVYIFITKWVMPPITIT
                     QISNSFSYGLKRDYVSWNKLGYNIKLAAIASEDQLFPDHNGFDWDAIEKSFKPKKGKK
                     KKSKIPLGGGASTISQQTAKNVFLWQGGGYVRKIPEFYFTQMIEWVWGKQRILEVYLN
                     VIEMGKGIFGVEAASQYYFKKPANKLSRTEAAMIIACLPNPKKFSVVPMSKRVAWRYP
                     QILQQMNYIEDDEDLLAIIK"
     gene            complement(2437..2829)
                     /locus_tag="PLU36_06655"
     CDS             complement(2437..2829)
                     /locus_tag="PLU36_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ46468.1"
                     /translation="MNFKLDTKEKFTSILIEEIDINANKAENLAAVCMPYLNNSVKNI
                     ILNLSNVVTIQEDATTILLKLQQIFYEKNASFVICCLQNAVENSLENYEVLEFLNVTP
                     TESEAWDIVQMEEIERELLDSEDIEFEA"
     gene            2917..5223
                     /locus_tag="PLU36_06660"
     CDS             2917..5223
                     /locus_tag="PLU36_06660"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HMZ46469.1"
                     /translation="MDNNFSSQVKEIISFSREEALRLGNNFIGTEHLLLGLIRDGENV
                     AIKVLKSLNVDLYALRKEIELAVKDKSGNSVANINSLPLTKQAEKVIRVTVLEAKSLK
                     SPLVETEHLLLSILKNKENIATQILNQFNIDYDLFRNELGMVGIAPINPPKSEYTDND
                     DDNDFEDEKRASYQSQKGTSKTPANSKSKTPVLDNFGRDITKLAESGSLDPIVGRENE
                     IERVSQILSRRKKNNPILIGEPGVGKTAIVEGLALRIVQRKVSRVLYEKRVISLDLAA
                     LVAGTKYRGQFEERMKAIMNELEKNRDVILFIDEIHTIIGAGGASGSLDASNIFKPAL
                     ARGELQCIGASTLDEYRMYIEKDGALDRRFQKIIVEPPSVEETIQILHNIKSKYEDYH
                     SVEYDDLSIEACVKLSDRYMTDRLLPDKAIDVLDEVGARVHLKNINVPKEIIELEKKI
                     EEIKNEKNKAVKSQSFEEAASLRDTEKRLGEDLEKAKNVWEEESKHKRFPITEEHIAE
                     VISMMTGIPVKRMVEAETEKLKKMSEDMKGMVIGQDEAILKVVKAIQRNRVGLKDPKK
                     PIGSFIFLGPTGVGKTELARSLARYMFDSEDALIRVDMSEYMEKFTVSRLIGAPPGYV
                     GYEEGGQLTEKVRRKPYSVILLDEIEKAHPDIYNILLQVLDDGVLTDGAGRKIDFKNT
                     LIIMTSNIGVRQLKEFGDGVGFATASRIQNQEENNKAVIEKALKKTFSPEFLNRIDDV
                     IIFNSLSKENIFSIIDILMKGVTKRLGN"
     gene            5262..5324
                     /locus_tag="PLU36_06665"
                     /pseudo
     CDS             5262..5324
                     /locus_tag="PLU36_06665"
                     /inference="COORDINATES: protein motif:HMM:NF021915.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5492..6193
                     /locus_tag="PLU36_06670"
     CDS             5492..6193
                     /locus_tag="PLU36_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ46470.1"
                     /translation="MNNSIIRLEDIFKNYYMGSQAITVLKGVSLEILKNEYVALMGPS
                     GSGKSTLMNILGCLDTATSGKYILNGNDVSKMTDDELAEIRNNEIGFVFQQFNLLPRL
                     SAAENVALPLVYAGVGKKERNERAMEALKMVGLEDRSHHKSNELSGGQIQRVAIARAL
                     VNNPALLLADEPTGNLDTKTSVEVMEIFGKIQQAGNTVVLVTHEEDIANYAKRVVKLR
                     DGLVETDILKEVAVM"
     gene            6220..>6491
                     /locus_tag="PLU36_06675"
     CDS             6220..>6491
                     /locus_tag="PLU36_06675"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HMZ46471.1"
                     /translation="MAMKIYTKTGDAGKTSLIGGTKVPKSHIRIESYGTIDELNSYIG
                     LVADYSSEEHIKIILKEIQDRLFTIGSSLATDPDKDPIMKLPDLKE"
BASE COUNT         2496 a          836 c         1229 g         1930 t
ORIGIN      
        1 aaaatggctg gcaactagta aagaagccaa tagtagatac tcaggtgaaa gagatttaga
       61 aaatccctta gctgcagtac aaatgggctt gatttatgta aaccctgaag gcccagatgg
      121 gaactctgat ccattagcag cagcagttga tatgagagaa acttttgcaa gaatggctat
      181 gaacgatgaa gaaactgttg cattaattgc aggtgggcat acttttggta aaactcatgg
      241 tgcaggtgat gttgctttgg taggccctga acctgaagca gcacctatag aagcacaagg
      301 tttaggttgg ttaagtaaac atggttctgg caaagctggt gatacaataa caagtggatt
      361 agaagtaacc tggactagca ctcctacaaa atggactaac aacttcttct ggaatttatt
      421 tggctatgaa tgggagttag aaactagccc tgctggtgca aaacaatggg ttcctaaaca
      481 tggcatgggt gcaaattccg ttccagatgc ccatgacccc aataaacgcc atgcccctaa
      541 aatgctcact acagatatgg ctttacgcct tgaccctact tatgaaaaaa tttcacgcag
      601 attttatgaa aacccagatg tatttgcaga tgcttttgct aaagcttggt ttaaattaac
      661 acatagagat atgggaccaa tctctagata tttaggaccc gaaattccta aagtagaatt
      721 tatttggcaa gatattatac ctgtaaatca acatgaatta attagtaaag atgatattgg
      781 ggcattaaaa gagttaattc tgcaatcggg tttaagtatt ggcgaaatgg tttctactgc
      841 atgggcttct gcatctactt ttagaataag tgataaaaga ggaggtgcaa atggtgcaag
      901 agtaaaatta gaaccacaaa aaaattgggt agtaaacaat ccaacccaat taacaaaagt
      961 catcagtact ctagaagcca ttcaatcaaa attcaatcaa tcaaacaaaa aagtatctat
     1021 tgcagattta attgtattag ctggtggtac agcaattgaa aaagctgcta aagatgctgg
     1081 atattctgta atagtgcctt ttatgccagg tagagcagat gcaactatgg aacagacaga
     1141 aattgattca attgcagtat tagagccatt tgcagatggg tttagaaatt acttaaaatc
     1201 aaaatcaact attgcaacag aagaattatt gatagataaa gcacaattgt taaacttaaa
     1261 tgctccagag ttaacagttt taattggagg tatgagaatg ttaaatacta attatgatgg
     1321 tagtaatcat ggtgtattta cttcaacacc agaaagattg accaacgact tttttgtaaa
     1381 tttattaaac ataaatactg tttggaaagc tattgataat acagaagaaa tatttgaagg
     1441 aacagataga acaacaggta ccaaaaaata tacagccaca agaaacgatt taatttttgg
     1501 ctctaattca gaattaaggg cattggcaga agtttatgct agtgaagatg caaaagaaaa
     1561 atttgttaat gattttgtta cagcttggac taaagtaatg aaccttgata gatttgattt
     1621 aaaaaataaa agctaaataa attggtttat taaaaaaaac tcattcaatt ttgaatgagt
     1681 ttttttgtgt aaaatattat taagcaatgt tgttattaca aaagttaaaa ttaaaaaatg
     1741 tttggaaatg gattaaacgt gttgtaataa tacttttcat aagttcttta gtgtatattt
     1801 ttattacaaa atgggtaatg ccacctatta caataacaca aattagtaat tcatttagct
     1861 acgggttaaa aagagattat gtaagttgga ataaattggg gtataatata aaattagcag
     1921 caattgcaag cgaagatcag ttatttccag atcacaatgg ctttgattgg gatgcaatag
     1981 aaaaaagttt taaacctaaa aaaggaaaaa agaagaagtc aaaaattcca ttaggtggag
     2041 gagcaagtac aatttctcag caaactgcaa aaaatgtttt tttatggcag ggtggaggat
     2101 acgtaagaaa aattccagaa ttttatttta cacaaatgat agagtgggtt tggggtaagc
     2161 aaagaatttt agaagtgtat ttgaatgtaa ttgaaatggg taaaggaatt tttggagtgg
     2221 aagctgcctc gcagtactat tttaaaaaac ctgcaaataa attatcacgt accgaagcag
     2281 caatgattat tgcttgttta cccaatccaa aaaagttctc tgttgtacca atgagcaaaa
     2341 gggttgcctg gcgttaccca caaattttac aacaaatgaa ttatatagaa gatgatgaag
     2401 atttgttagc aatcattaag taatagtaaa cagctattag gcttcaaact ctatatcctc
     2461 actatctaat aactctcttt ctatttcttc catttgtaca atatcccatg cttcactttc
     2521 ggtaggtgta acatttaaaa attctaaaac ttcatagttt tctaaactat tttcaacagc
     2581 attttgtaaa caacaaatta caaagcttgc atttttttcg taaaaaattt gttgcagttt
     2641 taataaaata gtagtagcat cttcttgaat ggttaccaca ttgcttaaat ttaaaataat
     2701 gttttttaca ctattgttta aatatggcat acaaactgct gctaaatttt cagccttatt
     2761 ggcatttata tctatttctt ctattaaaat agacgtaaat ttttctttgg tatcaagttt
     2821 gaaattcata attggttttc attaacatta tgtgaataaa tctgtattaa cttagctcaa
     2881 aaatattctt tatcattgta taaatccaaa catattatgg ataataattt ttcatcacaa
     2941 gtaaaagaaa tcatttcttt cagtagagag gaagcattaa gattaggaaa taactttatt
     3001 ggcacagagc atttgttgtt agggctaatt agagatggag aaaatgtagc catcaaagta
     3061 ttaaaaagcc ttaatgttga tttatatgcc ttacgcaaag aaatagaact tgccgtaaaa
     3121 gataaatctg gcaatagtgt tgctaatatt aatagtttac cactaaccaa acaagccgaa
     3181 aaagtaatta gagtaacagt tttagaggca aaatctttaa aaagcccttt ggtagaaaca
     3241 gaacatttgt tgcttagtat tttaaaaaat aaagaaaata ttgccactca aattttaaat
     3301 caatttaaca tagattatga tttattcaga aatgaattag gtatggtagg cattgcacct
     3361 atcaatcctc caaaatctga atatacagat aatgatgatg ataatgattt tgaagatgaa
     3421 aaaagagcaa gttatcaatc tcaaaaaggc acttcaaaaa caccggctaa ttcaaaaagc
     3481 aaaacacctg ttttagataa ttttggaagg gatattacaa agttggcaga atctggctca
     3541 ttagatccaa ttgttggaag agaaaatgaa attgaaagag tatctcaaat tttatcaaga
     3601 agaaagaaaa acaaccccat cttaattgga gaacctggtg ttggtaaaac agctattgta
     3661 gaaggcttag cattaagaat agtacaaaga aaagtaagta gagttttgta cgaaaaaaga
     3721 gttatttcat tagatcttgc agctttagtt gctggcacta agtacagagg acagtttgaa
     3781 gaaagaatga aagcaattat gaatgaatta gaaaaaaaca gagatgttat tttattcatt
     3841 gatgaaattc ataccataat tggtgctggt ggtgcaagtg ggagtttaga tgcaagtaat
     3901 atttttaaac ctgctttagc aagaggcgaa ctacaatgta ttggtgcaag cacactagat
     3961 gagtatagaa tgtatattga aaaagacggt gcattagatc gtcgttttca aaaaataatt
     4021 gtggaaccac caagtgtaga agaaacaatt caaatactac ataatattaa aagtaaatat
     4081 gaagattatc atagcgtaga gtacgatgat ttatctattg aagcttgtgt gaaattgagc
     4141 gatagatata tgacagatag attattaccc gataaagcaa tagatgtttt agatgaagtt
     4201 ggagcaagag tacatcttaa aaatattaat gttccaaaag aaataataga gctagaaaaa
     4261 aagattgaag agattaagaa cgaaaaaaat aaagctgtaa aaagccaaag ttttgaagag
     4321 gcggcatctt taagagatac agaaaaaagg ttaggagaag atttagaaaa agcaaaaaat
     4381 gtttgggaag aagaaagtaa acacaaacgt tttccaatta ccgaagaaca tattgcagaa
     4441 gtaatcagta tgatgacagg cattcctgta aaacgaatgg tagaagcaga aacggagaag
     4501 ttgaaaaaaa tgagtgagga tatgaagggt atggtaattg ggcaagacga agcaatattg
     4561 aaagttgtaa aagccattca aagaaatagg gtagggttaa aagaccctaa aaaaccaatt
     4621 ggctcattta tatttttagg accaacaggt gtaggcaaaa cagaattagc cagaagtctt
     4681 gcaaggtata tgtttgacag tgaagatgcc ttaataaggg tagatatgag tgaatatatg
     4741 gagaaattta ctgtaagtcg tttaattggt gctcctccag gctatgttgg gtacgaagaa
     4801 ggtggacaat taacagaaaa agtgagaaga aaaccttata gtgttattct attagatgaa
     4861 attgaaaaag cacatccaga tatttataat atcttactac aggttttaga tgatggtgtt
     4921 ttaacagatg gtgctggtag aaaaattgat tttaaaaata cactaatcat catgacctcc
     4981 aatattggtg ttagacagtt aaaagaattt ggagatggtg ttggttttgc tactgcttca
     5041 agaatacaaa atcaagaaga aaacaataaa gcagtaatag aaaaagcctt aaagaaaacc
     5101 ttttctccag agtttttaaa tagaattgat gatgtaataa ttttcaattc cctatctaaa
     5161 gaaaacatat ttagtattat tgatatttta atgaaagggg ttactaaacg cttgggcaat
     5221 taggctttac attagaacta acaaatgaag ctaaagactt tatagcagaa aaagggtatg
     5281 atgtacaata tggtgcaagg cctttacaca gggcaattca gaaatattta gaagacccat
     5341 tagcagatga aatattaaat accaaagtgc aagctggcga tatattaatt gctggatttg
     5401 ataaagaaaa taataaaatt gtttttgaat tcaaatcaag agaagaaaaa gaaaaagtat
     5461 aatttatata acattagaaa tttattttgt tttgaataat tcaattatta ggctagaaga
     5521 tatttttaag aattattata tgggtagcca ggcaattaca gtgcttaagg gtgtttcttt
     5581 agaaattctt aaaaatgaat atgtagcctt aatgggacca agtggtagtg gtaaaagtac
     5641 attaatgaat attttaggtt gtttagatac tgccaccagt ggcaaataca ttttaaacgg
     5701 aaacgatgta agtaaaatga ccgatgatga attagccgaa ataagaaata acgagatagg
     5761 ttttgttttt caacagttta atttattacc aagattatca gctgcagaaa atgttgctct
     5821 tccattagta tatgcaggtg taggaaaaaa agaaagaaat gaacgtgcaa tggaagcctt
     5881 aaaaatggta ggtttagaag atagaagcca ccataaaagc aatgaattaa gtggtggaca
     5941 aatacaacgt gttgctattg ccagagcctt ggtaaacaac ccagcacttt tattagcaga
     6001 tgagccaaca ggtaatttag atactaaaac atcggtagaa gtaatggaaa tttttggtaa
     6061 aattcagcaa gcaggtaata ctgtagtatt ggttacacac gaggaagata ttgccaatta
     6121 tgcaaaacgt gtagttaaat taagagatgg attagtagaa acagatatat tgaaagaagt
     6181 agcagtaatg taataagcaa cttctaaaaa ttatgattta tggcaatgaa aatttatact
     6241 aaaactggcg atgctggcaa gacttcttta ataggtggaa caaaagttcc taaaagccat
     6301 attagaattg aaagctacgg tacaattgat gaattaaatt cttatattgg attagttgct
     6361 gattattcat cagaggagca tattaaaata attttaaaag aaattcaaga tagacttttt
     6421 acaattggtt cttcgcttgc aacagacccc gataaagatc caataatgaa attacccgat
     6481 ttaaaagaag a
//
LOCUS       DAOQPK010000120         6455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_6891_length_6455_cov_4.157656, whole genome shotgun sequence.
ACCESSION   DAOQPK010000120 DAOQPK010000000
VERSION     DAOQPK010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6455
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6891_length_6455_cov_4.157656"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="PLU36_06680"
     CDS             complement(<1..775)
                     /locus_tag="PLU36_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HMZ46472.1"
                     /translation="MQKNWKIVEYDKALAETLQKELKINKTICKILVSRGIDNYTKAK
                     DFFRPTLEQLHSPWLMKDMDKATQRIIDAINKKEKILVYGDYDVDGTTSVACMYSFLK
                     AQHPFVDYYIPHRYKEGYGVSKAGIDFAAENNFSLIITLDCGIKSVDLINYAATLHLD
                     FIVCDHHMPDKILPNAIAILNAKQKDCNYPFKELCGCGVGFKLMQAIAEKLNLSKDSY
                     LQFVDLVAIAIAADIVPITGENRTLAYFGVKKVNENPNIG"
     gene            complement(788..1750)
                     /locus_tag="PLU36_06685"
     CDS             complement(788..1750)
                     /locus_tag="PLU36_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HMZ46473.1"
                     /translation="MKLALKVLEKELNLFELHFKDAVKSRVALLDRIMEYIVKRKGKQ
                     LRPMFVLLSAKLGGEVNNTSYRAASLVELLHTATLVHDDVVDEANQRRGFFSINALWK
                     NKIAVLVGDYLLSKGLLLSLNNKDFKVLQILSTAVKEMSEGELLQIEKSRNLNLKEDV
                     YYEIIKGKTASLLASSCAAGASSTFNDYDMIEKMRLFGEKTGMAFQIKDDLFDYGNSN
                     IGKPTGNDIKEKKLTLPIIYTLNNCNKETRKKIIYIIKNKNKEKEKVNYVIEQVKIHK
                     GIEYATEKMNLFRDEALSILYSFPESETRNALEELVRYTTDREF"
     gene            1947..3470
                     /locus_tag="PLU36_06690"
     CDS             1947..3470
                     /locus_tag="PLU36_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46474.1"
                     /translation="MTSKKYTLLAMIFLLFSVASFAQDTDWGWDWKDSSKIPAKRMPQ
                     HNEFLNNNYPYPAKPRSQWEFGFAGGPSFLIGDVNPKIGFGGSLTARKAINHTFSLRA
                     GYFGSMTSGKPSTFGTSVGQTEYKNQKHGLGFDLVVSLNPISYYRGNPKMNWYLTAGY
                     EFVATRVQYKDALGAWRTFYAYQYPNSQSGIIGTVGGDKHNGRRAWTLLHGASVGAGV
                     AYKINNKTNIKLEQRFTKIVTGYDLLDPVKGGKSDDMYSFTSFGVNLNMGKTAKRVQP
                     LWWLNPNNFAYNELNKPKHMKMPKVVLDDADSDGITDQFDLEPNTPAGAKVDARGRAV
                     DTDGDGIPDYKDKEILTSQKCFPVNNDGVGTCPEPPCCKELRDEITKIKESGAATNKA
                     NTCEIGELPSIQFKGNSKLNKDAQAVLATVAQKIIANPNCKIKVTGYGSTSKSAQQAS
                     WEKVNAVIKYLVEKQGIAESRLIFTYGQDGDANTVDLQGTTEDGPNSVPAPHPNLRK"
     gene            3677..5416
                     /locus_tag="PLU36_06695"
     CDS             3677..5416
                     /locus_tag="PLU36_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ46475.1"
                     /translation="MTKIKLILSLVVFIVFSNTINAQLGGVHNYSDSSYISPRDMKQQ
                     SDFMGNKGSFPSKPRDQWQFGIFAGYPYVDGDCATTFKGLGKGLQAYSYGFGISLRKA
                     MGYVFSVRGSFAYYNMLGLDYQPNSNFNNSQVIRNLYENAPRGYIHNHRTMAFTPSLE
                     GLVSLSNIMFHTKQKRWNIYGLFGYGALIYNTKMNMMKPDGSSYATEFQEVANAYTSG
                     VKRVKLRAMLREKLDGSYETMADVNDRRPGLNKNWHLRHCFTSGVGIEYRMGKRWSMT
                     FEYKRMQTRDDYVDGWYRQSGDLRYAVFTSEWDNVAFAGLGANFNIGNSKKRVPPLWW
                     LNPLEFAYADLANNKAAIAKMFKVKDDDNDGVMNELDLEPNTPEGCPVDTHGVTADTD
                     GDGVPDCKDKEKLTLQKCFPVDADGVGNCPEPACCDSLANKIQKCCTGTVVGNKSTEC
                     TLEELPSIQFYANSYKLSKDAEAILSVVADKMKNNPNCNLKVVGYGNKTKAQQQLSWD
                     RVNAIIRYLVEQQGIAENRFIFTYGNAADGDILSVDLLPTMETGPNTVQPPHPQLRKA
                     SNPAKKKGLFNKN"
     gene            5569..6387
                     /locus_tag="PLU36_06700"
     CDS             5569..6387
                     /locus_tag="PLU36_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46476.1"
                     /translation="MRNSITMEHHFERNKNIQASVFTVFICLLIFFIFFYIRWSLPII
                     EKPLLTEGIEVNIGNSDVGSGEVQPLVQGEPGPESNQTPEQPTTPTNTAPATEQSTEK
                     DDPDAVATHTNKTTKVTKPVAVAPVNKPKTVMPKYSGGNSNGGNSKDNYNDSRSEGND
                     KSGVGDKGKPWGSIDGTVYDGPGGTRVYGLGKRRITRFPTFTDDFSENAKVAINIVVD
                     KNGNVTYAAVNPNGTSTANKQTRAVALKRAKEIKFETSEQEQRGTIILDMRVSG"
BASE COUNT         2220 a          995 c         1100 g         2140 t
ORIGIN      
        1 taccaatatt aggattttca tttactttct taactccaaa ataggcaagt gttctgtttt
       61 cgcctgttat tggaacaata tctgcggcta tagctatagc aaccaaatca acaaactgca
      121 aataactatc cttacttaaa tttagttttt cagcaattgc ctgcataagc ttaaagccaa
      181 caccacagcc acataattct ttaaatgggt agttacaatc tttttgttta gcatttaaaa
      241 tagcaatagc attaggtaaa atcttatctg gcatgtgatg gtcgcaaaca ataaagtcta
      301 aatgtagggt tgctgcataa tttattaagt caacactttt tatgccacag tctaaagtaa
      361 taattaacga aaaattattt tcagcagcaa agtcaatacc tgctttactt acaccatatc
      421 cttctttata acgatgtgga atgtaataat ctacaaacgg atgttgagcc tttaaaaagc
      481 tgtacataca ggcaacactt gttgtcccat caacatcata atcgccataa actaaaattt
      541 tttctttctt attaatagca tctattattc tctgtgttgc tttgtccatg tccttcatca
      601 accatgggct atgcaattgt tctaaagtgg gtctaaaaaa atcttttgct ttagtataat
      661 tatcaatacc tctactaact aaaattttac aaatggtttt attaattttt agttcttttt
      721 gtaaagtctc tgctaaggct ttatcatatt ctactatttt ccaatttttt tgcatgataa
      781 aaaaattcta aaattcccta tctgttgtat atctcacaag ttcctctaaa gcatttctgg
      841 tttcagattc aggaaagcta tacaaaatac ttaaggcctc atctctaaat aaattcattt
      901 tttctgttgc atattcaatg cctttatgta ttttgacttg ttctattaca taatttactt
      961 tctctttttc tttattttta tttttgataa tataaattat tttttttctt gtttctttat
     1021 tacagttgtt gagtgtatat ataattggta gagttaattt tttttctttg atatcattac
     1081 ctgtaggctt accaatattg ctattgccat aatcaaacaa atcatctttt atttgaaaag
     1141 ccataccagt tttttctcca aataatctca ttttttcaat catgtcatag tcattaaaag
     1201 tagaggaagc accagctgca caagatgaag ctaataagga tgcagttttg cctttaatta
     1261 tttcgtaata aacatcttct tttaaattta agtttctaga tttttcaatt tgtaaaagct
     1321 ctccttcact catttctttc actgctgtag aaagaatttg taaaacttta aaatctttat
     1381 tatttaaaga gagtagtaac ccttttgata aaagataatc gccaactaaa actgcaatct
     1441 tatttttcca tagggcatta atagaaaaga atcctcttct ttgattagcc tcatcaacca
     1501 catcatcatg caccaaagta gcagtatgca gcaactctac taaacttgca gccctatagg
     1561 atgtgttgtt tacctcgcct cctaattttg cagaaagtaa aacaaacata ggccttagct
     1621 gcttgccttt tctttttact atatactcca ttatcctatc taataaagca accctgcttt
     1681 ttactgcatc tttaaaatgc aattcaaaaa ggtttaattc cttttctaaa acttttaagg
     1741 ctaatttcat ttaacttggc taataagttt gcaatataat tatatataga attgaagaat
     1801 tttttattat tgggaagcat tactttcctt aatatcaggt ttaaattaac tttaaatatt
     1861 attttttggt tttttagttg ctttaattac ttttgcttcg tattaaagtc taaaaatttt
     1921 caatatttaa cttataaatt ttcactatga caagcaaaaa gtacacatta cttgctatga
     1981 tttttttatt attttctgta gcaagttttg ctcaagacac agactggggg tgggattgga
     2041 aagatagttc caaaattcct gctaaaagaa tgcctcagca caatgagttt ttaaacaata
     2101 actaccctta tcctgctaaa cctcgttctc aatgggagtt tggttttgct ggtggtccat
     2161 catttcttat tggtgatgta aaccctaaaa ttggttttgg tggttcttta actgcacgta
     2221 aagctattaa ccatactttt tcattaagag ctggttattt tggttctatg acttctggta
     2281 aacctagcac ttttggtact tcagtaggac aaacagagta taaaaatcaa aaacacggct
     2341 taggatttga tttagttgtc tctttaaatc ctattagtta ctacagaggt aacccaaaaa
     2401 tgaactggta tttaacagct ggctatgaat ttgtagcaac aagagttcaa tacaaagatg
     2461 ctcttggtgc ttggagaaca ttttatgctt accagtaccc taattcacaa tctggcataa
     2521 ttggtacagt tggtggagac aaacacaatg gaagacgtgc ttggacttta ttacatggtg
     2581 caagtgttgg tgcaggtgtt gcttacaaaa taaacaacaa aacaaatatt aaattagagc
     2641 aaagatttac caaaatagtt actgggtatg atttgttaga tcctgtaaaa ggtggtaaaa
     2701 gtgatgatat gtatagcttt actagttttg gtgttaactt aaatatgggt aaaacagcta
     2761 aaagagtaca gcctttatgg tggttaaatc caaacaactt tgcatataac gagttgaaca
     2821 aaccaaaaca catgaaaatg cctaaagtgg tgttagatga tgctgatagc gatggtatta
     2881 cagaccaatt tgatttagaa ccaaatactc ctgcaggtgc aaaagtagat gctcgtggac
     2941 gtgcagttga tacagatggt gatggtattc ctgattataa agacaaagaa attcttactt
     3001 ctcaaaaatg tttccctgta aataatgatg gtgttggaac ttgtccagaa cctccttgtt
     3061 gcaaagaatt aagagatgag ataactaaaa ttaaagagtc tggtgctgca acaaataaag
     3121 caaatacttg cgaaattggt gaattaccaa gcattcaatt taaaggtaat tcaaaattga
     3181 ataaagatgc tcaagctgta ttagcaactg tagctcaaaa aattattgca aacccaaatt
     3241 gtaaaataaa agttaccggt tatggctcta ctagcaaatc ggctcaacaa gcaagttggg
     3301 aaaaagtaaa tgctgtaatt aaatacttag tagaaaaaca aggtattgca gaaagcagat
     3361 taatttttac ttatggccaa gatggagatg caaatactgt tgacttacaa ggtacaacag
     3421 aagatggacc aaatagcgtt ccagctcctc atccaaattt gagaaaataa ttagtagaaa
     3481 ctagtaataa tgtgcataaa attggcttcc tgaaaaaagg aagccttttt tatacaccca
     3541 tgtggaataa tttattgaaa attaccttga tttgcaacct attgttacaa ttaattgtct
     3601 gtatcttgcg tttaattact taattgtaat tttatccaac ctgtgaaagc cgtaaaaaaa
     3661 gttttatttt ttaattatga ctaaaattaa actcattctt tcactggttg tgtttatagt
     3721 attttcaaat acaataaatg cccaattagg cggagtgcac aattattcag attcttctta
     3781 tatctcacca agagatatga aacaacaatc tgattttatg ggtaataaag gaagttttcc
     3841 atctaaacca agagaccaat ggcagtttgg catttttgct ggctatcctt atgtagatgg
     3901 tgattgtgct actactttta aaggattagg taaaggactg caagcttatt cttatggctt
     3961 tggtattagt ttacgaaaag caatgggata tgttttttcc gttagaggtt catttgccta
     4021 ttataatatg cttggcttag attaccaacc taactcaaat tttaacaata gccaggtaat
     4081 tagaaattta tatgaaaatg ccccaagagg ttatattcat aaccatagaa caatggcatt
     4141 tactccttcc ttagaaggat tagtttcgtt aagtaatatt atgtttcata ccaaacaaaa
     4201 acgttggaac atttatggct tatttggata tggtgcctta atttacaata ctaaaatgaa
     4261 tatgatgaaa ccagatggta gttcttatgc cactgagttt caagaagtag caaatgcata
     4321 tactagcggt gtaaaaagag tgaaattaag agccatgcta agagaaaagt tagatggatc
     4381 ttacgagaca atggcagatg tgaacgatag aagacctggt ttaaacaaaa attggcattt
     4441 aagacattgc tttacctctg gtgttggaat tgaatataga atgggtaaga gatggtccat
     4501 gacttttgaa tataaaagaa tgcaaacaag agatgattat gtggatggtt ggtatagaca
     4561 aagtggcgat ttaagatatg cagtttttac atcagaatgg gataatgtgg cttttgctgg
     4621 tttaggagct aattttaaca taggaaactc caaaaaacgt gttccgccac tatggtggtt
     4681 aaacccactt gaatttgctt atgccgattt agcaaataat aaagcggcaa ttgcaaaaat
     4741 gtttaaggtg aaagatgatg acaatgatgg tgtaatgaat gaacttgacc ttgagccaaa
     4801 cacaccagaa ggttgccctg tagatacaca tggtgtaact gcagatacag atggtgatgg
     4861 agtacctgat tgtaaggata aagaaaaact taccttacaa aaatgtttcc ctgtggatgc
     4921 agatggtgtt ggtaactgtc ctgagcctgc ttgttgtgat tctttagcta acaagataca
     4981 aaaatgttgt actggaacag tagtaggaaa taaatctaca gaatgtactt tagaagaatt
     5041 accatccatt cagttttatg ctaacagtta caagttaagt aaagatgctg aagcaattct
     5101 ttcagtagta gcagataaaa tgaaaaataa tccaaattgt aatttaaaag tggttggtta
     5161 tggtaataaa acaaaagcac aacaacaatt aagttgggat agagttaatg ctattatcag
     5221 atacttagta gaacaacaag gcattgcaga aaatagattt atttttactt atggaaatgc
     5281 tgcagatgga gatattttat ctgttgattt attaccaaca atggaaactg gaccaaatac
     5341 cgttcagcca ccacatcctc agttaagaaa agcaagtaac ccggcaaaga aaaaagggtt
     5401 gtttaataaa aactaattct ttaatacaat aattaataaa gccccattta atttgaatgg
     5461 ggttttattt tacttaactt ttttttatgt ataaaagtga atagagtcat caagtaaatt
     5521 gtaattttca acatttacca aaagtttcat tattaaattt aagtttgtat gcgtaattca
     5581 ataacaatgg aacatcattt tgaaagaaat aaaaacattc aggcatcggt ttttaccgta
     5641 tttatttgtt tgcttatttt cttcattttc ttttatataa gatggagttt accaattatt
     5701 gaaaaacctt tattaacaga aggtatagaa gtaaatattg gtaatagtga tgtaggaagt
     5761 ggagaagtac aaccattggt acaaggagag cctgggccag aatctaatca aactccagag
     5821 caaccaacca caccaacaaa tacagcacct gccacagagc agagtacaga gaaagacgac
     5881 cctgatgcag ttgcaaccca taccaataaa acaactaaag ttaccaaacc tgttgcagta
     5941 gcacctgtta ataagcctaa aactgtaatg ccaaaatata gtggtggtaa tagcaatggt
     6001 ggtaatagta aagataatta taacgacagt agaagtgagg gtaacgataa atcgggtgtg
     6061 ggagataaag gcaagccttg gggaagtatt gatggaactg tttatgatgg accaggtgga
     6121 acaagagttt atggtttagg taaaagaaga ataacacgct ttcctacatt tacagatgac
     6181 ttttcagaaa atgcaaaagt tgctattaat attgtggtag ataaaaacgg aaatgtaacg
     6241 tatgccgctg taaatcctaa tggtacaagc actgcaaata aacaaactag agctgttgca
     6301 ttgaaaagag caaaagaaat taaatttgaa acaagtgagc aagagcaacg aggtactatt
     6361 attttagaca tgagagtttc tgggtaactt gctttaatta acttatgaac tataaagagt
     6421 ctgttgacta tttatttgaa caactaccta tgttt
//
LOCUS       DAOQPK010000121         6453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_6892_length_6453_cov_5.225070, whole genome shotgun sequence.
ACCESSION   DAOQPK010000121 DAOQPK010000000
VERSION     DAOQPK010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6453
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6892_length_6453_cov_5.225070"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="PLU36_06705"
     CDS             complement(<1..360)
                     /locus_tag="PLU36_06705"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HMZ46477.1"
                     /translation="MNKWMKYKIIFVFSCLLFCTIVKANNTHKYIGKYKDLVEELSKE
                     FGIPRSIITAVSIIESGAGRSRNCKLLKNHFGIIGRNNLLKTKGIKTRFKQYETDEDS
                     FRDFCKLLSRRKYYKKLK"
     gene            469..1329
                     /locus_tag="PLU36_06710"
     CDS             469..1329
                     /locus_tag="PLU36_06710"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ46478.1"
                     /translation="MKRFFVFISFLLFLVACKKNITDNSASVAALTLTDVAYASANSN
                     QKLDVYLPANRSTATTYTCIMVHGGSWSGGDKSDFNTDIATLKTLVGNYAIININYSL
                     ANGSSILLQQQIDDLNAAFNFIHSKANEYLINTNKIAIIGASAGAHLSLLKAYKYNTD
                     NKIKAMVDLFGPTNMTWMYTSHPYPTIAQPTIVNVMGATPITNAAAYYNGSPINFVSS
                     TVPPTIIFHGTADEIVPISESVNLKNALATAGVQYEYYTYTGEAHGWTGANLTDTYNK
                     VITFIKNYVK"
     gene            complement(1346..2068)
                     /locus_tag="PLU36_06715"
     CDS             complement(1346..2068)
                     /locus_tag="PLU36_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HMZ46479.1"
                     /translation="MKKFIAILVVTIFTSSIQAQVIKGLINKVTNKDKNAKSGLSTEE
                     VVQGLKEALTVGAEKSANQLSNVDGFFKNEAIKILLPPEAKKVQDALSKIPGGSKYVE
                     DAILSMNRAAEDAAKSAAPIFINAIKNMSFADAWEILKGSDSAATKYLKQQTSKPLTD
                     AFRPIIDKSLAKVNATQHWNTVFSTYNKFSFKKINPDLASYVTEKALEGIFYQLAQEE
                     LKIRKDPVAQTTNLLKKVFGGK"
     gene            2248..4188
                     /gene="dnaG"
                     /locus_tag="PLU36_06720"
     CDS             2248..4188
                     /gene="dnaG"
                     /locus_tag="PLU36_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HMZ46480.1"
                     /translation="MITPQTIEQIKSRIDIIDIISQYVNLKKRGSNYIGNCPFHHEKT
                     PSFTVSPTKELYKCFGCGKSGNAITFLMEHDKLSFVEALKWLAAKYNIEIEETETTAE
                     QKIVTQTADSLYILNNFATNFLEKELWQGEEGVAIALTYLKERGFREDIIKKFQLGYN
                     PTQKDKLVTTLLNNQFNKELIPKSGLGVERNQQELVDNYRGRIIFPIHNNTGKIIGFG
                     ARVIGNAKDKAPKYINTPENEIYIKSKILYGIYFARSPIDKANECLLVEGYTDVISLH
                     QAGIENVVASGGTSLTTEQIRIIKKFTSNLTIIYDGDSSGIKAALRGLDMALEEGLNV
                     KLVLIPDNEDPDSFVNKVGTTAFKEFIKNNKKDFIIFQLEVILKDAGNDIVLKNNAVN
                     NIADTLSKINRPEDFTKLQEYIKQCAAILKIDELGLTNLVNKFKADKLAKDAKKQANN
                     TTFQQQEFPEQTINTSNDDVSILFNQDDESERNLLKVLLEHGLKQFNQEQTIAEFIFN
                     ELNTFYFQNNLIETIVEEYRTAYNEGKQPEPKTFLYHTNNEIRNLVIAITATPYELSV
                     RWDDKLQGMNIVNRDTSTQDALISVNFFKLRKIKKMIDENQHDLEVETDLRKQEYYIT
                     VHKELKETEKNITQTLGTVILK"
     gene            complement(4202..4765)
                     /locus_tag="PLU36_06725"
     CDS             complement(4202..4765)
                     /locus_tag="PLU36_06725"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HMZ46481.1"
                     /translation="MMRDLNWKTLSSKYIYKDRWFIARADSCLMANGKTIEPYYVLEF
                     PNWCNVIVVTNKEEIVMVRQYRHAINKTTIELPGGVIDKNENPKEAAIREVLEETGYT
                     IHQIEFLYATAPNPATNNNMAYFFLAKANENLQCQQPDPFEDLDVILVDKKEVLEMIH
                     NSQIIHGVQIGAIYAAYQKLGWIEQKI"
     gene            complement(4816..5805)
                     /locus_tag="PLU36_06730"
     CDS             complement(4816..5805)
                     /locus_tag="PLU36_06730"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HMZ46482.1"
                     /translation="MNKITAAITAVGGYLPETKLTNADLEKMVDTTDEWIKTRTGIHE
                     RRILKEPGKANSDMIVPAIQEILNKKGLSPLDIDCIICATVTPDYVFPATANVVSDKL
                     GATNAWGYDLGAACSGFLFALTTGAALIESGRYKKVVVAGADKMSSIIDYTDRNTCII
                     FGDGAGAVLLEPNSEGNGVLDSILKSDGSGREFLHMKAGGSYSPATIETVMAKQHFVY
                     QEGKTVFKFAVKGMADVSAELLERNNLTGNDIAWLVPHQANLRIIDATAERVGLPKEK
                     VMINIQRYGNTTSGTIPLCLWEWEKQLKKGDNLVLAAFGGGFTWGAILVKWAY"
     gene            5954..>6453
                     /gene="ruvA"
                     /locus_tag="PLU36_06735"
     CDS             5954..>6453
                     /gene="ruvA"
                     /locus_tag="PLU36_06735"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HMZ46483.1"
                     /translation="MIAFIRGSFIQKQPARVVVDVNGIGYDLQISLNTYSYIANKDQG
                     QLFTYLHITENAQTLYGFADLEEKELFLNLISVSGVGAATARMMLSGMKPEEIARAIV
                     QGNTKQLESIKGIGKKSAERLIVELKDKLSKNASTALTTITNALPGYDAVNALVSLGI
                     AKPMAE"
BASE COUNT         2279 a         1020 c          943 g         2211 t
ORIGIN      
        1 tttaagcttt ttataatatt ttctgcggct taataattta caaaaatctc taaacgaatc
       61 ttcatctgtt tcatattgtt taaatctggt tttaatgccc tttgttttaa gtaaattatt
      121 ccttcctatt ataccaaaat gattttttag cagcttacaa tttctgcttc ttccagcacc
      181 agattcaata atacttacag cagtaattat acttcttgga atgccaaatt ctttagataa
      241 ctcttcaacc aggtctttat attttccaat atacttatgt gtattatttg cctttactat
      301 tgtacaaaaa agtaaacaac taaaaacaaa aattatttta tacttcatcc acttattcat
      361 actattaata agttgctttt taagctcttt taagtattat tttaaacaag ataatcattt
      421 cttataaaat tctttaattt gggcatctat ttattcaata ataataatat gaaaaggttt
      481 tttgttttta tttcatttct actatttctt gtagcttgta aaaaaaatat aacagataat
      541 agtgcttcag tagcagcttt aacgttaaca gatgttgctt atgctagtgc aaattctaat
      601 caaaagttag atgtgtattt gccagctaat agaagtacag caactactta tacttgtata
      661 atggttcatg gaggaagttg gagtggtgga gacaaatctg attttaatac agatattgca
      721 accctaaaaa ctttagtagg taactatgct ataataaata ttaattatag tttggctaat
      781 ggtagctcaa tattattaca acaacaaata gatgacctta atgcagcatt caattttatt
      841 catagcaaag ccaacgaata tttaattaac accaataaaa ttgcaataat tggtgctagt
      901 gctggtgcac atttatctct tttaaaagca tataaataca atactgataa taaaattaaa
      961 gcaatggtag atttatttgg gccaaccaat atgacttgga tgtacacaag tcatccttat
     1021 cctacaattg cacaaccaac aatcgttaat gtaatgggtg caacgcctat taccaatgca
     1081 gcagcttatt ataatggtag ccctataaat tttgtttcct ctacagtgcc tccaacaata
     1141 atttttcatg gcacagccga tgaaattgta cccattagcg aaagtgtaaa tttaaaaaat
     1201 gcactcgcaa ccgcaggagt acaatatgaa tattatactt acactggaga ggctcatggt
     1261 tggacaggtg caaaccttac agatacttat aataaagtaa ttacttttat taagaactat
     1321 gttaaataat attttttaat aaaaattatt tgccaccaaa aactttttta agcaggttgg
     1381 tggtttgtgc aacagggtct tttcttattt ttaattcttc ttgtgcaagc tgatagaaaa
     1441 taccttctaa agccttctct gttacataag aagccaaatc tggatttatt tttttgaagg
     1501 aaaatttgtt ataggttgaa aagactgtat tccaatgctg tgttgcattt actttagcta
     1561 aagatttgtc aatgataggt ctaaaagcat ctgttaatgg tttgctggtt tgttgtttta
     1621 aatattttgt tgctgcagag tcgctacctt ttaaaatttc ccaagcatct gcaaagctca
     1681 tatttttgat ggcattaata aaaataggtg cagcactttt tgcagcatct tcagctgcac
     1741 ggttcataga taaaattgca tcctctacat acttacttcc accaggtatt ttactaagtg
     1801 cgtcttgcac ttttttagcc tcgggaggta ataaaatttt aattgcttcg ttcttaaaaa
     1861 agccatctac attagatagt tgattagcac ttttttctgc accaactgtt aaagcttctt
     1921 ttaaaccttg tactacttcc tctgtactta aacctgattt tgcgttttta tctttattgg
     1981 ttactttatt tattaaacct ttaattactt gtgcttgaat actgcttgta aaaattgtaa
     2041 caactaaaat ggctataaat tttttcataa taattttttg gggtataaat atccatactt
     2101 tttaagtatt gaatcagctt taaaaatatt ttaaattctt tataacataa ttaccaaaat
     2161 aatgccataa ttacatatac caatatctat atttatctaa ttcaatttat accacacttt
     2221 aaacctttac ttttgtagca ttctacaatg ataacgccac aaaccataga gcaaattaaa
     2281 agcaggatag atattataga tatcattagc caatacgtta atctaaaaaa gcgtggtagt
     2341 aattatattg gcaattgccc ttttcatcac gaaaaaactc catcttttac tgtatctcct
     2401 actaaagaat tgtataaatg ttttggttgt ggcaaaagtg gtaatgcaat aactttttta
     2461 atggaacatg acaaactaag ttttgtagaa gcattaaaat ggttggctgc taaatacaat
     2521 attgaaattg aagaaacaga aacaacagca gagcaaaaaa ttgtaacaca aactgcagat
     2581 agcttatata tcttgaataa ctttgctacc aattttttgg agaaagaact atggcaagga
     2641 gaagaagggg tagctattgc attaacttat ttaaaagaaa gaggctttag agaagatatc
     2701 ataaaaaaat ttcagttagg atataatcct acccaaaaag ataagttagt tactacatta
     2761 ctcaataacc aatttaataa agaattaatt ccaaaatctg gtttaggtgt tgaaagaaat
     2821 cagcaggaat tagtagataa ttacagaggc agaattattt ttcctattca taataatact
     2881 ggtaaaataa ttgggtttgg agcaagagta attggaaatg caaaagataa agcaccaaag
     2941 tatattaata caccagagaa tgagatttat ataaaaagta aaattttata tggtatatat
     3001 tttgctcgtt cacctataga taaagctaat gaatgtttat tggtagaagg ttatacggat
     3061 gtaataagct tgcatcaagc aggaattgaa aatgtagttg ccagtggtgg tacttcttta
     3121 actacagaac aaattagaat aataaaaaaa tttaccagca acctcaccat tatttatgat
     3181 ggcgatagct ctggaataaa agctgctttg cgtggtttag atatggcttt agaagaaggc
     3241 cttaatgtaa aacttgtttt aataccagat aatgaagacc ctgatagttt tgtaaataaa
     3301 gttggtacca ctgcttttaa agagtttata aaaaataata aaaaagattt tataattttt
     3361 cagttagagg taatactgaa agatgctggt aacgatattg ttttaaaaaa taatgcagta
     3421 aataatattg ccgataccct aagcaaaatt aacaggccag aagattttac caagcttcaa
     3481 gaatatatta aacagtgtgc tgctatttta aaaatagatg aattaggctt aactaatcta
     3541 gtaaataagt ttaaagcaga taaacttgca aaagatgcta aaaagcaagc aaataatacc
     3601 acttttcaac aacaagaatt tccagaacaa acaataaata ctagcaatga tgatgttagc
     3661 attttattta accaagatga cgaaagcgaa agaaatttat taaaggtttt actagagcat
     3721 ggtttaaaac aatttaatca ggaacaaaca attgcagaat ttatttttaa tgaattgaat
     3781 actttttatt ttcaaaacaa tttaattgaa accatagttg aagaatatag aactgcatac
     3841 aacgaaggca aacaacccga accaaaaaca tttttatacc atactaataa cgaaataaga
     3901 aaccttgtaa ttgccattac agctacacct tatgaactaa gtgttagatg ggatgataag
     3961 ttacaaggaa tgaatattgt taacagagac acttctacac aagatgcttt aataagtgta
     4021 aactttttca agctcagaaa aatcaagaaa atgatagatg aaaatcaaca tgatttagaa
     4081 gtagaaacag atttaagaaa gcaagaatat tatattacag tacataaaga gttaaaagaa
     4141 accgaaaaaa atattaccca aactttagga actgttattc taaaataaat tacaggtatt
     4201 gttatatttt ttgttcaatc caacctaact tttgatatgc agcataaatg gcacctattt
     4261 gtacaccatg tataatttgg ctattgtgta tcatttctaa aacttctttt ttatccacta
     4321 aaataacatc taaatcttca aaagggtctg gttgttgaca ttgtaaattt tcattggctt
     4381 tagctaaaaa aaaataagcc atgttattat tagtagcagg gtttggtgct gtggcataca
     4441 aaaactctat ttgatgtatg gtatatcctg tttcttctaa aacttctctt atagcagcct
     4501 ctttggggtt ttcgttttta tcaattacac cacctggcaa ttcaatagta gttttattta
     4561 tagcatgcct atattgccta accattacaa tttcttcctt attagttaca acaattacgt
     4621 tacaccaatt aggaaattct aaaacataat atggttcaat tgtttttcca ttagccatta
     4681 aacaactatc tgcacgtgca ataaaccatc tgtctttata tatgtattta gaggataaag
     4741 ttttccagtt taaatctctc atcattcact ttataagtta caaataataa ctatttagta
     4801 gtaggtagaa aaaaattaat atgcccattt taccaaaata gctccccatg taaaaccacc
     4861 accaaaagca gctaaaacaa ggttgtctcc ttttttaagt tgcttttccc actcccataa
     4921 acataaaggt atagtgccag aagtggtatt accataacgt tgtatgttaa tcatcacttt
     4981 ttctttaggt aaacctacac gttctgcagt tgcatcaata attctaaggt tagcttgatg
     5041 aggtactaac catgctatat cattgcctgt taagttattt ctttctaaaa gttcagcact
     5101 cacatcagcc attcctttta ctgcaaattt aaaaactgtt ttcccctctt ggtacacaaa
     5161 atgttgtttt gccattactg tttctatagt tgcagggctg taagagcctc ctgctttcat
     5221 gtgtaaaaac tcacggcccg acccatcact ttttaaaata ctatctaaca ctccatttcc
     5281 ttcactattg ggttctaata aaacagcacc agcaccatca ccaaaaatga tacatgtatt
     5341 tctatcagta taatctatta tggagctcat tttatctgca ccagctacaa ccactttttt
     5401 atatctacca ctttcaatta atgcagcacc agttgttaat gcaaataaaa agccactaca
     5461 agctgcccct aaatcgtatc cccaggcatt ggtagctcct aatttatcag aaactacatt
     5521 tgctgttgca ggaaaaacat aatctggtgt tacagtagca caaataatac aatctatatc
     5581 taaagggctt agtccttttt tgtttaatat ttcctgaatt gcaggcacaa tcatatcact
     5641 atttgccttg ccaggttctt tcaaaattct tctttcatga atacctgttc tggttttaat
     5701 ccattcgtcg gtagtatcta ccattttttc taaatcggca ttggttagtt ttgtttctgg
     5761 caaatagcct ccaactgctg taatagctgc tgtaatttta ttcatttaat tatttatttc
     5821 taattataaa tttaagtaac aaatatccat atttacttaa gagaatagga atattgtttt
     5881 acgtaaaatg ttttaactac aaatttatgt tcatatatta tataaaccct tatttttgaa
     5941 atccttgtaa attatgatag catttattag aggaagtttt attcaaaaac aacctgcccg
     6001 agtggtggtt gatgtaaatg gtattggcta cgatttgcaa ataagtttaa atacttattc
     6061 ttatatagcc aataaagacc aagggcaact gtttacatat ttacatatta ccgaaaatgc
     6121 acaaacgctt tatggttttg cagatttgga agaaaaagaa ttgtttttga acttaataag
     6181 tgtatctggt gtaggtgcag caactgcaag aatgatgttg tctggtatga agccagaaga
     6241 aatagctaga gcaattgtac agggcaacac taaacaatta gaaagtatca aaggaattgg
     6301 taaaaaaagt gcagaaagat taattgtaga gttaaaagat aaactgagca aaaatgcttc
     6361 aacagctcta actactataa ccaatgcatt acctgggtac gatgctgtta atgcattagt
     6421 atccttaggt attgcaaaac ctatggcaga aca
//
LOCUS       DAOQPK010000122         6356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_7093_length_6356_cov_4.481193, whole genome shotgun sequence.
ACCESSION   DAOQPK010000122 DAOQPK010000000
VERSION     DAOQPK010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6356
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7093_length_6356_cov_4.481193"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..342)
                     /gene="iscX"
                     /locus_tag="PLU36_06740"
     CDS             complement(103..342)
                     /gene="iscX"
                     /locus_tag="PLU36_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HMZ46484.1"
                     /translation="MNFEPPIHWNDYEDIALKLYERFGDDFSESKIYRIRFTDLLEWV
                     LQIPGFVGTREQSNEGHLEMIQSSWVYEWRDNQPK"
     gene            complement(445..1023)
                     /locus_tag="PLU36_06745"
     CDS             complement(445..1023)
                     /locus_tag="PLU36_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HMZ46485.1"
                     /translation="MKEILLIDNYDSFTYNLVHLIEKITSLKPIVYLNDKIDLEAVNQ
                     YETIILSPGPGIPKEAGIMLPLIKDYAKTKKILGVCLGHQAIAEAFGASLINLPKVFH
                     GVATECFVDKTNNSLFQNITQPFMVARYHSWAVSKNNFPENLEIIAEDKDGIVMALQH
                     KIYNVKGIQFHPESILTPLGENILRNWIEDIK"
     gene            1124..1411
                     /locus_tag="PLU36_06750"
     CDS             1124..1411
                     /locus_tag="PLU36_06750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ46486.1"
                     /translation="MEILHNTSKPISCFYIKEGDVVLAKIDYKVEEDNTIFILHTEVS
                     EKLKGMNIGNKLLQKVADLARSEHRKIVPLCSFALAMFNKKEEYKDVWKQA"
     gene            1446..2036
                     /locus_tag="PLU36_06755"
     CDS             1446..2036
                     /locus_tag="PLU36_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMZ46487.1"
                     /translation="MGRIKTTYNLSKKEIILEKAAQLIKEKGFKAASMRDLAEIVGVE
                     AASLYNHIKNKNELLYEICLNVADLYNQQIALIETEEVSNLEKIEKLLRFHIQCMVIN
                     FEKVFVSDREWKHLSEPYLSNFQMQRRNYRKRFAALINDGIYKNEIKKIDATTAVLIM
                     LYAVSGIESWHRSKQKISGIELEENMIAILVAGLKK"
     gene            2075..3151
                     /gene="paaK"
                     /locus_tag="PLU36_06760"
     CDS             2075..3151
                     /gene="paaK"
                     /locus_tag="PLU36_06760"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HMZ46488.1"
                     /translation="MSLHFYPLTIKQVNQETANCVSVLFDVPENLQATFAYKHGQNIT
                     VKTTINQQEVRRSYSLCSTPLSNEFRIAIKKLDGGLFSTFANEGLKEGDVLEVMPATG
                     RFFTEINAHHQKNYVAFAAGSGITPIISIIKTILLAEPKSTVTLIYGNKNVSSIIFKE
                     ELDAIKNNFLQRFQLLHILSQEGTDAPVFSGRINEEKLQQLSAIINYNKVDNFFVCGP
                     EAMIFTIKNYLEQLGIENNKIHFEIFVSSTPNLNKNSSKKIIDSKEATSKIVIKADGR
                     SFDFDLPFNSASILDGALKHGADLPFACKGGMCCTCKAKVLEGKVEMDVSWGLGADEI
                     EQGYILTCQSHPITSKVVVDYDIK"
     gene            complement(3278..4540)
                     /locus_tag="PLU36_06765"
     CDS             complement(3278..4540)
                     /locus_tag="PLU36_06765"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HMZ46489.1"
                     /translation="MFALVDCNNFYASCERVFRPDLNEKPIVVLSNNDGCVIARSNEA
                     KALGIPMGAPAFEYEKIFQQNNIQVFSANFALYGDMSQRVMNILSEYTPNIEMYSIDE
                     CFLQLTGFDAHFNLQQYGNTMCKKVLQYTGIPISVGIATTKSLSKVANKIAKKYTKQT
                     GGVYIIDNEEKRLKALKWLKIEDVWGIGRQHSKRLHAIGITTAFAFTQLDNAYVQKQF
                     SIVELRLKQDLQGIPTLGLEEVQAKKSIATTRTFENNYTTVEELIERISTFAVSCAEK
                     LRKQNACCNSLMVFIHSNRHRKDLPQYNRSIVIKLPFATNSTIELAKFAVQALKLIFK
                     QGYAYKKAGVIVQDFVTENNIQQTLFENRNPKHISLMKAIDQLNVNFGQQKIRLASQD
                     QKRVWKMKQEKLSPRYTTNLADIITVHT"
     gene            complement(4541..5023)
                     /gene="umuD"
                     /locus_tag="PLU36_06770"
     CDS             complement(4541..5023)
                     /gene="umuD"
                     /locus_tag="PLU36_06770"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HMZ46490.1"
                     /translation="MFYKPYNLPITLPPLKLHTSKTIDIYSALSETALELPFVGQNIS
                     AGFPSPALDFTDLGIDLNKHLIKHPSATFYGRAKGQSLKNAGINDGDLLIIDRSVEPI
                     NGKIAVCYIDGEFTAKRIKKTKNELWLMPENEDYKPIKIEEENNLIIWGIVTHVIKAV
                     "
     gene            complement(5182..5255)
                     /locus_tag="PLU36_06775"
     tRNA            complement(5182..5255)
                     /locus_tag="PLU36_06775"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5219..5221),aa:Pro,seq:ggg)
     gene            complement(5323..6282)
                     /locus_tag="PLU36_06780"
     CDS             complement(5323..6282)
                     /locus_tag="PLU36_06780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HMZ46491.1"
                     /translation="MRKIERIIINLQPVAFIIKKGKEIKPPGFSGLAIYDVIMFLFTH
                     LNKVGLNERAAAISFNFIMAIPAACIFIFTLVPYIPIAKHFNAELLKLTADITPNQNT
                     YYFIKDFLNDFFNKPRTGLLSFGFLLVIFYASNAMMGIIRAFDKSVKLQKQYFFQLRI
                     RAIKLTTLLVLLVIATILVLIGHEQLAALIKSIFKTKSKLTWWGSVRWIIILALVFYS
                     IALIYKYAPSINKRWKLITPGSLLATCLILITTILFSFWVNNFSSYNKIYGSIGTVLI
                     IMILIYINSLILLIGFELNVSIMQLKLNAEAREKNEQLQSKPS"
BASE COUNT         2235 a          896 c          969 g         2256 t
ORIGIN      
        1 gccatctgtt agtacgccat ccatatcaaa aacaaatgtt ttaattttac taaattgttc
       61 aagcacattc attgtaaaaa atagatttga aaataaaatt atttatttag gttggttatc
      121 tctccattca tatacccaac tactttgtat catttctaaa tgtccttcat tactttgctc
      181 acgtgtgcct acaaagccag gaatttgtaa aacccattct aataaatctg taaatcttat
      241 tctgtaaatt ttactttcgc taaaatcgtc gccaaaacgt tcatatagtt taagagcaat
      301 atcttcataa tcgttccaat gaataggtgg ttcaaaattc attcttcaaa aattttaaaa
      361 ttataaaatg caaatatgct tttaattcgg catttaatag ctaaacaatg gaatatactt
      421 ttttaatata aaaatctact tgctttattt aatgtcttca atccaatttc ttaaaatatt
      481 ttcacctaaa ggagttaaaa tactttctgg atgaaactga atgcctttaa cattataaat
      541 tttgtgctgt aatgccatta caattccatc tttatcttct gcaataattt ctaaattttc
      601 aggaaaattg tttttactta ctgcccatga atgataacga gcaaccatga aaggttgtgt
      661 aatattttgg aataaactgt tgtttgtttt atctacaaaa cattctgttg caacgccatg
      721 aaatactttt ggcaaattta ttaaacttgc tccaaatgct tctgcaattg cctgatgccc
      781 taaacaaact cctaaaattt tttttgtttt agcataatct tttattaatg gtagcattat
      841 accagcttct ttaggaattc caggtcctgg agataaaata atggtttcgt attgatttac
      901 tgcttctaaa tcaattttat catttaaata tactatgggc ttaagtgaag taattttttc
      961 aattaaatgc actaggttgt atgtaaaaga gtcgtaatta tctattagca aaatttcttt
     1021 catggcttaa agcttgtatg cccaataatt tatcttcaat aatacaaaat caaagtttaa
     1081 aaactaaaca attttaaagt aactttactg taaacaattt ataatggaaa ttttacacaa
     1141 cacaagtaag ccaataagct gtttttatat aaaagaagga gatgttgttt tagctaagat
     1201 tgattataag gttgaagagg ataatactat ttttattctt cataccgaag tgagtgaaaa
     1261 attaaaaggt atgaatattg gtaataagct acttcaaaaa gtagcagatt tggccagaag
     1321 tgaacacagg aaaattgtac ccttatgtag ttttgcattg gcaatgttta ataaaaaaga
     1381 ggaatataaa gatgtgtgga aacaagctta atttataata agcttttata tatttttgta
     1441 ataatatggg tagaattaaa actacataca acctaagcaa aaaagaaatt atacttgaaa
     1501 aagcagcaca gttaataaaa gaaaaaggct ttaaggctgc aagcatgaga gatttggctg
     1561 aaattgttgg tgtagaagca gccagcttat ataaccatat aaagaataag aatgaactat
     1621 tgtatgaaat atgtttaaat gtagccgatt tatacaatca gcaaattgct ttaattgaaa
     1681 cagaggaagt aagcaattta gaaaaaattg aaaaattatt acgttttcat attcaatgca
     1741 tggttataaa ttttgaaaaa gtatttgtaa gcgatagaga atggaaacat ttatctgaac
     1801 cttatttatc taactttcaa atgcaaagaa gaaattacag aaaacgattt gctgcattaa
     1861 ttaatgatgg tatatacaaa aatgaaataa aaaaaattga tgctactacc gcagtgttaa
     1921 ttatgttgta tgctgtaagt ggcattgaaa gctggcatag aagtaaacaa aaaatttcag
     1981 gtattgaatt agaagaaaat atgattgcaa ttttagtagc tggcttaaaa aaataaactt
     2041 ttaatatcaa ccaataatat ttataattta aaatatgtct ttacactttt atcctttaac
     2101 tattaagcaa gtaaatcaag aaactgccaa ttgtgtttcg gttttatttg atgttccaga
     2161 aaatttacaa gcaacatttg cttacaagca tgggcaaaat ataactgtaa aaactactat
     2221 taatcaacaa gaggtaagaa ggtcttattc cttatgctct acgccattaa gtaatgaatt
     2281 tagaattgca attaaaaaat tagatggtgg tttgttttca acttttgcca atgaaggctt
     2341 aaaggaagga gatgttttag aggtaatgcc ggcaacaggc agatttttta cagaaattaa
     2401 tgctcatcat caaaaaaatt atgttgcttt tgctgctggt agtggtataa ccccaattat
     2461 ttctatcatt aaaactattt tattagccga gcctaaaagt actgtaacgc ttatttatgg
     2521 taacaaaaat gtaagctcta ttatttttaa agaagaatta gatgctataa aaaataattt
     2581 tttacagagg tttcagttgt tgcatatttt atcacaagag ggtacagatg ctcctgtttt
     2641 ttcaggcaga attaatgaag aaaaactaca acaactttcg gcaattatta attataataa
     2701 agtagataat ttttttgttt gtggaccgga agctatgatt tttactatca aaaactattt
     2761 agaacaatta ggaattgaaa ataataaaat tcatttcgaa atttttgttt cttcaactcc
     2821 aaatcttaat aaaaacagtt ctaaaaaaat aatagatagt aaggaagcca caagtaaaat
     2881 tgtaattaaa gccgatggta gaagttttga ttttgattta ccctttaaca gtgccagcat
     2941 tttagatgga gctttaaaac atggtgccga cttgcctttt gcctgcaaag gaggtatgtg
     3001 ttgtacttgt aaagcaaaag tgttagaagg caaggtagaa atggatgtaa gctggggttt
     3061 gggtgcagat gaaattgaac aagggtatat tttaacatgc caaagtcatc ctattacaag
     3121 taaggtagta gtagattatg atataaaata gtttgaattt aaatagctgc aaagtgttgg
     3181 taaggtggtg ttatactagg ctattttaca aaaaattatt aaccatattt tgaatattta
     3241 tttatactta tttaattggt ttaactatat tcaaatttca agtatgcaca gttataatat
     3301 ctgctaaatt ggttgtatat cgtggcgata gtttttcttg tttcattttc caaacacgct
     3361 tttgatcttg tgatgctaaa cgaatttttt gctgaccaaa atttacattc aactggtcta
     3421 ttgctttcat taaagaaatg tgtttagggt ttctgttttc aaataatgtt tgttgtatat
     3481 tattttctgt tacaaaatct tgtacaataa caccagcttt tttataagca taaccttgtt
     3541 taaaaattaa ctttaatgct tgtacggcaa actttgctaa ttcaatagta gagtttgtag
     3601 caaaaggtag tttaataaca atacttctgt tgtattgtgg caagtctttt ctatgcctat
     3661 tgctatgtat aaaaaccatt aatgagttac aacaagcatt ttgttttctt agtttttcgg
     3721 cacaagaaac agcaaaagtg gatatacgtt ctatcaattc ttctactgta gtataattat
     3781 tttcgaaggt acgggtagtg gcaatacttt ttttagcctg cacttcttct aaaccaagtg
     3841 tagggatgcc ttgtaaatct tgttttaatc taagctctac aatagaaaat tgtttttgta
     3901 cataagcgtt atctagctgt gtaaatgcaa aagcagtagt tatacctatg gcatgtaagc
     3961 gtttgctatg ttgtcttcca ataccccaaa catcttctat ctttagccat tttaatgctt
     4021 ttaatctttt ttcttcgtta tcaataatat atacaccacc tgtttgcttg gtatattttt
     4081 tagcaatttt attagcaact ttagagagag atttggtagt agcaatacct acactaatgg
     4141 gtatgcctgt atattgtaat acttttttgc acattgtatt gccatactgt tgcaagttaa
     4201 aatgtgcatc aaatccggtt agttgtaaaa aacattcatc aatactatac atttcaatat
     4261 tgggggtgta ctcacttaat atattcatta cccgttggct catatctcca tataaagcaa
     4321 aattggctga gaatacttgt atattatttt gttgaaatat tttttcatat tcaaaagctg
     4381 gtgcacccat tggaatgcca agagctttgg cttcattact tctggcaata acacaaccat
     4441 cgttgttaga taatacaaca ataggcttct cattcaaatc tggccgaaaa actctttcgc
     4501 aagaagcata gaaattatta caatcaacta aagcaaacat ttaaacagct tttataacat
     4561 gggttacaat accccaaata attaaattat tttcttcctc aatttttata ggtttataat
     4621 cttcattttc tggcattagc cataactcat tttttgtttt tttaattcgc ttggcagtaa
     4681 attcaccatc aatataacaa acagcaattt taccatttat tggttctaca cttctgtcaa
     4741 ttattaataa atcgccatcg ttaatgcctg cattttttaa agattgccct ttagcacgcc
     4801 cataaaatgt agctgatggg tgcttgatta aatgcttgtt taagtctata cccaaatctg
     4861 taaaatcaag ggcaggagag ggaaaacctg cactaatatt ttgccctaca aatggtagtt
     4921 caagggctgt ttcacttaaa gcggaataga tatctattgt tttgcttgta tgtagtttta
     4981 atggcggcaa tgttataggt aaattgtatg gtttataaaa caaattgcta gtgaaaataa
     5041 aattattttt taataattga gtacatctga atatatattt tttgtgtact agtttaatgg
     5101 attgattgga cagaaaaaca agtgcatttt taaaaaaagg taaggctttg gctataagcc
     5161 aaagcctttg ttgtatagtt gtcgggtgga ctggattcga accagcgacc ccttgcaccc
     5221 catgcaaata cgctaccgga ctgcgccacc acccgtaact tttttattgt ttgcaaatat
     5281 atgttctgaa aaatattttt taataaataa acaagggttt atttatgagg gtttgctctg
     5341 taattgttca tttttttctc tggcttcggc atttaatttt agttgcataa tgcttacatt
     5401 taattcgaag cctattaata aaattaaaga gttaatataa attagaatca taataattaa
     5461 tacagtacca attgagccat aaattttatt gtacgatgaa aaattattta cccaaaatga
     5521 aaataaaata gttgtaatta atattaaaca agtggcaagc agagagccag gagtaattaa
     5581 tttccaccgt ttattgatag aaggggcata tttataaatt aaagcaatac tataaaaaac
     5641 caatgctaaa ataattatcc atcttacgct gccccaccaa gtaagtttag attttgtttt
     5701 aaaaatactt tttattaatg cagctaactg ttcgtgccct attaaaacta aaatggtagc
     5761 aataactaat aaaaccaata atgttgtaag ttttattgcc cttattctta actgaaaaaa
     5821 atattgtttt tgtagtttta ctgatttatc aaaagctcta ataataccca tcatggcatt
     5881 ggatgcataa aatattacta ataaaaaacc aaaggatagt aaacctgttc ttggtttatt
     5941 aaaaaaatca tttaaaaaat cttttataaa atagtaagtg ttttggttgg gtgtaatatc
     6001 ggctgtaagt tttagcagct ctgcattaaa atgtttagca atgggtatgt aaggtactaa
     6061 agtaaaaata aaaatacaag cagccggtat agccataata aaattaaacg aaatggctgc
     6121 tgctctttca tttaaaccaa ctttattaag gtgggtaaat aaaaacataa ttacatcata
     6181 aattgctaaa ccgctaaagc caggtggctt tatttcttta ccttttttaa taataaaagc
     6241 aacaggttgt aagtttatta taattcgttc tatttttctc acaataagta aattaataaa
     6301 ctaaagatag tataaaccta tttctgaact tcgctactaa aatgaaattc aatatc
//
LOCUS       DAOQPK010000123         6355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_7099_length_6355_cov_4.208571, whole genome shotgun sequence.
ACCESSION   DAOQPK010000123 DAOQPK010000000
VERSION     DAOQPK010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7099_length_6355_cov_4.208571"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PLU36_06785"
     CDS             complement(<1..263)
                     /locus_tag="PLU36_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase subunit L"
                     /protein_id="HMZ46492.1"
                     /translation="MPKDNSIKSVLIVGSGPIIIGQACEFDYSGTQAARSLREEGIKV
                     ILINSNPATIMTDPMMADKVYLLPLTVESIEQILEENQIDAVLP"
     gene            581..1042
                     /gene="bcp"
                     /locus_tag="PLU36_06790"
     CDS             581..1042
                     /gene="bcp"
                     /locus_tag="PLU36_06790"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HMZ46493.1"
                     /translation="MTILTIGKKAPAFTGKDQNSNKVSLADFKGKKVVLYFYPKDSTP
                     TCTVQACNLRDNFALLKQQGIEIIGVSADDIKSHQKFANKNKLPFSLIADTAKIIIEK
                     YGVWAEKKLFGIKYMGIRRTTFVIDEKGIILAIITKPKSKSHAEEILKVIA"
     gene            complement(1092..2186)
                     /gene="gldN"
                     /locus_tag="PLU36_06795"
     CDS             complement(1092..2186)
                     /gene="gldN"
                     /locus_tag="PLU36_06795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HMZ46494.1"
                     /translation="MKKLFLGIVVISSVIGFKNDAFAQFKKAKKTEEKTDAGKTGTTG
                     TTGTGGVTSTTSTSTSTAKKDTTIIKPGKRFDTTRDVSGKEYGGVVPTSKRNAYGYTK
                     EPVSDRKPLPYDHIREDDATFSHFVWREIDAREKMNLPFIYSAKDETGDQRFFSIILN
                     AIKNDSVVAFDAMDDRFTTPLSINQVISMSSAQSTLLDTTYAENPNDENVMDTVIKWK
                     ENLKAPKPDSIYKFRIKEQWMFDREASKFLVRIIGIAPLAPNPLPPGVKKSNAPPTYY
                     PLFWVYYPDLRPTLSRTFAYNPKNIGGQLSWEEVFEGRFFSSYIIKSTVNNPGDKTLA
                     QLIEDPLFRLLEGENIKEKLFNYEQDLWSY"
     gene            complement(2214..3743)
                     /locus_tag="PLU36_06800"
     CDS             complement(2214..3743)
                     /locus_tag="PLU36_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldM family protein"
                     /protein_id="HMZ46495.1"
                     /translation="MYLVLTALLALNVSSEILNAFKTVKSSLESSSELANGKTQQIFE
                     SFKDKMEDPTTKEQATIWYEKANQVHTLSNNVFKYVEDLKLELMKEAGFDPNNKKDGK
                     TDSSFKEDNLEAATRLLVETAPQGKGKGKELFKKLQDFKSALFGLDSAVVEELGKSLP
                     IDLSMPKTKTLGNNTWEAAYFRMTPTIAALTILSKFQNDIRNTEAQVVELFHKKVGEV
                     KIKYDQFKAIANASASYVMPSEEIIINAGVGAFSSAAKPSVTIDGAGAQALPDGSYEY
                     KFNAPTAAGEYTKKVRISFVKPDGTTATVDKDIKYTVGQLAGLTVSTDATRVFYSGGI
                     ENPLSVTGQGGSEKIQLSVSGTGVTVESRGKGQYIVKCTQPGAAILKASDGKSTQEFK
                     IPIKRVPDPVIMVNGNRGGSMEAGKFKAAGGVSAKLLDFIFEGYTFKVSSFALFFTGT
                     GFEEKNEVVEVQGNAFNAEARRFMNRCAAGSTVVIGAVRVADQIGNVREVEGTISFTL
                     E"
     gene            complement(3867..4634)
                     /gene="gldL"
                     /locus_tag="PLU36_06805"
     CDS             complement(3867..4634)
                     /gene="gldL"
                     /locus_tag="PLU36_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HMZ46496.1"
                     /translation="MAAKIPPKVGKIVDVLVSIAAAIVLFGALQKLMHTKLADLFLQI
                     GLYSEAAIFLGYGILYMYYPAMDEHEVHVPGLEGVEKDNNPLKAMEKMMKEADITPAN
                     LGKLSAGFEKFGSTVSQISDLGDVVKSTSDFSSKTKEAATALGSVAGAVSQATVSLGS
                     FNEASESTKQFHGQVQVLTKNLASLNTIYELELQESNNHLKALNQFYGKLAEAGAAMN
                     GTAEDAEKAKQQIAALAGNLSKLNQVYGNMLTAMAGR"
     gene            complement(4744..6042)
                     /locus_tag="PLU36_06810"
     CDS             complement(4744..6042)
                     /locus_tag="PLU36_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HMZ46497.1"
                     /translation="MRNSLSALLLPALLISLVACNKKGKVKGTPDNGQLVGVAPSAKP
                     NTGRLAGLVYIPPGTFHMGPSDEDINFNYTARNKQVSINGFWMDNTEITNNEYRQFVY
                     WTRDSIALKELGYVKNTNGGGGGDSSIAVDWSKKKDIKYDQSTLEKLGNKLMIDAPER
                     LYGKIEVDPKKIVYHIEYFDLTEAARIENKDIPRTNFIKRFDQQIYPDTLVWLRDFSY
                     AYNEPMTKRYFSHPSFGNYPVVGVNWKQAVAFSHWRTHYLNSYLGKKKKATESDFRLP
                     SEAEWEYAARGGRTNSMYPWGNYYLRNKKGCLLANFKPGRGNYPEDGGFYTVRADAYW
                     PNDFGLYNMSGNVAEWTGSYYYEGAYNLIHDMNPDIRYDAKDSDPPRMKRKVVRGGSW
                     KDVGYYLQTGTRSYEYQDTAKSYIGFRCVIDLAPRSGKRK"
BASE COUNT         1975 a         1146 c          900 g         2334 t
ORIGIN      
        1 ggtaacactg catctatttg gttttcttct aaaatttgtt caatactttc tactgttaat
       61 ggtagcaaat aaactttatc tgccatcata gggtcagtca ttatggtagc agggttacta
      121 ttaattaaaa taactttaat cccctcttct cttaaacttc ttgcagcttg agttccacta
      181 taatcaaact cgcaagcctg accaataatt attggaccac tgccaacaat taaaacagat
      241 tttatggaat tatctttagg cataatttta tttttattta cttagctttt atattgctat
      301 ttggcttttg ttgcgtcgca ctcttgtaca ccttgttctt ttctgtactt gtcatgccaa
      361 caataggtgg catctactca actattttac aaaaactcaa caggtttctc caatagtcta
      421 aataatatcc tctgtacatt agtggaaaca gaaaaaacaa attgtgcaaa tgtacggcaa
      481 taggcttttt aattgctttt ttacttaaac tatttttatt ctaacaacca tctaaatgat
      541 ttatttttat tctttcaaca atcaattaat ttttatttaa atgacaatac ttacaatagg
      601 caaaaaagct cctgctttta caggtaaaga tcagaatagc aataaagttt ctctggcaga
      661 ttttaaaggg aaaaaagtag tactttattt ttatcctaag gatagtacac ctacctgtac
      721 agtgcaagct tgtaacctaa gagataattt tgcattatta aagcagcaag gtatagaaat
      781 tattggtgtt agtgcagatg atattaaaag tcatcaaaaa tttgcaaata aaaataaatt
      841 accattttca ttaatagcag atacagcaaa aattattatt gaaaaatatg gcgtttgggc
      901 tgaaaaaaaa ttatttggta taaaatatat gggaattaga agaaccacat ttgtaattga
      961 tgaaaagggt attatcctgg caattattac aaaaccaaaa agcaaatcac atgcagaaga
     1021 aattttgaaa gttatagcat aaaaaatccc acttaattat ttaagtggga tttactaaaa
     1081 ttatatctca attagtacga ccataagtct tgttcgtaat taaataattt ttctttaata
     1141 ttttcccctt ctaataatct aaacaatgga tcttcaatta attgagccaa tgttttgtcg
     1201 ccagggttat taacggtaga ttttattata tagctactaa aaaatcttcc ttcaaatacc
     1261 tcttcccaag aaagttggcc accaatattt ttgggattat aagcaaatgt tcttgatagt
     1321 gttggtctta aatcaggata ataaacccaa aataaaggat agtaagttgg tggagcattg
     1381 ctttttttaa caccaggagg taaagggttt ggtgctaaag gtgcaatacc aattattctt
     1441 actaaaaatt tactagcttc tctgtcaaac atccactgtt ctttaattct aaatttataa
     1501 atagagtctg gtttaggagc ttttaaattt tctttccact taattacagt atccattaca
     1561 ttttcatcat tggggttttc tgcataagta gtatctagca aagtagattg agcagatgac
     1621 atactgataa cctgattgat ggataaagga gtagtaaacc tatcatccat tgcatcaaaa
     1681 gcaacaacac tgtcgttttt aatagcattc aatattatgg aaaagaatct ttggtctcca
     1741 gtttcgtctt tagcagagta aataaatggc aagttcattt tctctcttgc atctatttct
     1801 ctccaaacaa aatgactaaa tgtagcatca tcttctctaa tatgatcgta tggtaaaggt
     1861 tttctatcgc ttaccggttc tttggtatat ccataagcat ttcttttgct tgtaggaact
     1921 acaccaccat attctttacc agaaacatct ctagttgtat caaaacgttt tccaggttta
     1981 attatagtag tgtctttttt agcagttgag gtgctagtgg atgtagttga agtaactcca
     2041 ccagtaccag ttgtacctgt tgtacctgtc ttaccagcat ccgttttctc ttctgttttt
     2101 ttggcttttt taaactgtgc aaatgcatcg tttttaaaac ctattacaga agaaataaca
     2161 actataccca aaaacagttt tttcataata aagaattaat ttataattta caattactct
     2221 aatgtaaagg agatagtacc ttcaacctct cttacattac caatttgatc agccacacga
     2281 acagcaccaa taacaactgt agaacctgct gcacatctat tcataaatct tcttgcttct
     2341 gcattaaatg cattaccttg tacttctaca acctcatttt tttcttcaaa accagttccg
     2401 gtaaagaata atgcaaaaga agatacttta aaggtatatc cttcaaatat aaaatctaat
     2461 aatttggcag atacaccacc agcagcttta aacttaccag cttccatact accaccacga
     2521 ttaccattta ccataattac agggtctggt acacgtttaa ttggaatttt aaactcttgt
     2581 gtagatttac catcacttgc ttttaaaata gcagcaccag gttgtgtaca tttaactata
     2641 tattgtcctt ttcctcttga ttcaacagtt acacctgtac ctgatacact caactgtatt
     2701 ttttcagagc caccttgacc agtaacacta agtgggtttt caataccacc agagtaaaat
     2761 actcttgtag catctgtgga tactgttaaa ccagctaact gaccaacagt atatttaata
     2821 tctttgtcaa cagtagctgt tgttccatca ggctttacaa aacttattct tactttctta
     2881 gtatattcac ctgcagcagt aggggcatta aatttatact cgtagctacc atcaggcaaa
     2941 gcttgagcac cagcaccatc aatagttaca ctaggtttag cagcactact aaatgcacca
     3001 acaccagcat taattattat ttcttcgcta ggcattacat aagatgcaga agcattggca
     3061 atagctttaa attggtcgta tttaattttt acttcgccca cttttttgtg gaataattca
     3121 accacttgtg cttctgtatt tctaatatcg ttttggaatt tacttaatat tgttagagca
     3181 gcaattgttg gagtcattct aaaataagca gcctcccaag tgttattacc taaagttttt
     3241 gttttaggca tagataaatc aattggtaag cttttgccta actcttctac tacagcacta
     3301 tctaaaccaa ataatgcaga tttgaaatcc tgtaatttct tgaataactc ttttcctttt
     3361 ccttttcctt gtggagcagt ttcaactaat aatcttgttg ctgcttctaa gttatcttct
     3421 ttaaatgaag agtctgtttt gccatctttt ttgttgtttg gatcaaaacc agcttctttc
     3481 attaattcta attttaaatc ttctacatac ttaaatacgt tattgcttaa agtatgtact
     3541 tgattggctt tttcgtacca aatagtagct tgctctttgg tagttgggtc ttccatttta
     3601 tctttaaaag attcaaatat ttgctgagtt ttgccattag ctaactcact tgaactttct
     3661 aagctacttt ttacagtttt aaaagcgttt aatatttcag atgatacgtt taatgctaat
     3721 agtgcagtta acactaagta cattaagtta atcatcttct gccgtggctc cttaggtagt
     3781 gccatgttgt attattttat ttaattcaat ttacaaatta ggtttatctc gtttaatttc
     3841 ttgtatttat cgtaatacaa tagaaattat ctaccagcca ttgcagttag catattacca
     3901 taaacttggt taagtttact taaattacca gctaatgcag caatttgttg tttagctttt
     3961 tccgcatctt ctgctgtacc attcattgca gcacctgctt cagctaattt accatagaat
     4021 tggtttaaag cttttaagtg gttattactt tcttgtaatt ccaattcata aattgtattt
     4081 aatgatgcta agtttttagt taaaacttga acttggccat gaaattgttt agtactttca
     4141 gaagcctcat taaatgaacc aagagaaaca gttgcttggc taacagcacc agctacactt
     4201 cctaatgcag ttgcagcttc tttagtttta cttgaaaaat cagaagttga tttaacaaca
     4261 tctcctaaat cgcttatttg tgaaactgta gaaccaaatt tttcaaaacc agcacttaat
     4321 ttacctaaat tagctggtgt aatatctgct tctttcatca ttttttccat tgcttttaat
     4381 ggattattat ctttttctac accctccaaa cctggcacat gcacttcgtg ttcgtccatt
     4441 gcaggatagt acatgtatag aataccataa ccaagaaata ttgcagcctc tgaatacaaa
     4501 cctatttgaa gaaataagtc tgctaattta gtatgcatta atttttgtaa agctccaaat
     4561 aatactattg cagctgcaat agatactaat acgtcaacga ttttaccaac tttaggagga
     4621 atttttgctg ccatgtttta gatttttaaa tttttgtggt tttatattta ctgaataaat
     4681 agactttttt ttattgtaca taaggttgta caacttcttt attaattaat tcttattatt
     4741 agtttatttt cttttacctg atcttggtgc taggtcaatt acacaacgga accctatata
     4801 tgattttgct gtatcttgat attcatatga tcttgttccg gtttgtaagt aataacctac
     4861 atctttccaa ctgccccctc taacaacttt tcttttcatt cttggagggt cgctatcttt
     4921 tgcatcgtaa cgaatatctg ggttcatgtc atgaattagg ttgtatgcac cttcataata
     4981 gtatgaacct gtccattctg caacattacc agacatatta tataatccaa aatcatttgg
     5041 ccaatatgca tcagctcgta ctgtgtaaaa ccctccatct tctgggtaat taccacgacc
     5101 tggtttaaag ttagctaata aacatccttt tttatttctt aaatagtagt taccccaagg
     5161 atacatagag tttgttctgc ctccacgagc agcatactcc cactcagctt cacttggtaa
     5221 tctaaaatca ctttcagttg cctttttctt tttacctaaa tagctattca ggtaatgtgt
     5281 tctccaatga gagaaagcta ctgcttgttt ccagttaaca cctactacag gataattacc
     5341 aaatgatggg tgactgaaat atcttttagt cattggttca ttataggcat aagagaaatc
     5401 tcttaaccaa accaatgtat ctggataaat ttgttggtca aatcttttta taaaattagt
     5461 tctaggaatg tctttatttt caattctagc agcctctgtt aaatcaaaat attcaatatg
     5521 gtaaactatt tttttaggat ctacttcaat ttttccatat aatctttcag gagcatctat
     5581 cattagttta tttcctaatt tttcaagagt gctttgatca tatttaatat cctttttctt
     5641 gctccaatca acagcaatag aagagtctcc tcctccacca ccattggtgt ttttaacgta
     5701 gcctaactct tttaatgcaa tggaatctct tgtccaataa acaaactgac gatattcgtt
     5761 attagtaatt tcagtattat ccatccaaaa accattgata gatacttgtt tattacgtgc
     5821 agtatagttg aagtttatgt cttcatcgct tggccccata tgaaaagttc ctggtggtat
     5881 gtaaaccaaa ccagcaagcc ttccagtatt gggctttgca cttggtgcaa cgccaactaa
     5941 ctgaccatta tcaggtgttc ctttcacctt accttttttg ttacaggcta ctaaacttat
     6001 cagtaaagca ggtaacagga gagcacttaa actatttctc attctacaaa ataattgaag
     6061 ggtagtacag tgtacaaata tatagatatt ttttttgtcg caaatttttt ttcataaaac
     6121 aaccctatta agggcattta ataacgattg attttaaaac ttattgtatt atataaatgt
     6181 attttggtag aattttaacc tatagatagt ttttatgtat ttcaaggtct aaattaagcc
     6241 aacacattgt tatacaattg ctaaaagtgc ttcaatagta tcaaatggtt tgtaacaact
     6301 gttatttcgg cacaaataaa aggtattttt ttctattaaa aacctatttt ttaat
//
LOCUS       DAOQPK010000124         6331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_7144_length_6331_cov_4.413002, whole genome shotgun sequence.
ACCESSION   DAOQPK010000124 DAOQPK010000000
VERSION     DAOQPK010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7144_length_6331_cov_4.413002"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="PLU36_06815"
     CDS             complement(<1..372)
                     /locus_tag="PLU36_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46498.1"
                     /translation="MKKYISLALIYAILITLSVSSCNKRLENNVSHEISGQTINASDI
                     PLLTDEVVAKIEEEKSHLEILKDEICLQVANIAQVVTPTGDGSLTYPNWVRFVEIAEA
                     IDSLNDNWNEAYGQALDNIINT"
     gene            716..1090
                     /locus_tag="PLU36_06820"
     CDS             716..1090
                     /locus_tag="PLU36_06820"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ46499.1"
                     /translation="METIGQRIAQERFAKRWTQEKLASELGITQSLLSDIENDKVSPK
                     WQTILSTAEKLEVPFSKLLPNDSNILYQPSMNDNSIGFVHNYNSFADERKLWEALLQA
                     KDEIIQSQKLVIATLQEQGKKG"
     gene            1344..3329
                     /locus_tag="PLU36_06825"
     CDS             1344..3329
                     /locus_tag="PLU36_06825"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HMZ46500.1"
                     /translation="MKSIWIYLFVALGFIAAVFPPEQKKVALIIAIGDYSEAPGWRNL
                     SSMNDVRYVKQALLKVGFATNNIDTLINKDATKQGMIKALDDLYNKVGEGDIVYFQFS
                     GHGQQIQDDNGDELDGYDEALIPYDASAMYDPVTYSGDKHFRDDLLGEKLNKIRKKIG
                     PKGSLVVLVDACHSGTATRDAGIKRGMEKPFLTDPKYVPNIKINLNKEGEHGLLEGFT
                     NGMGNMIVFSASSPNQPNYEMKDNNNNGVGSLSYAFAKSIAELPAESSYRVLFHKMKA
                     IIQGDIPNQIPMVEGDLDLEVFGGKYIPKPTIIAVDQKFNNASNKFNDTTFVISIGQL
                     NSIYKGTTLKIYPVGSAEAYCDAVVTSVSSFQSICKSNKPLKKSVAYEAKVEAASAGD
                     FAAELMIQNNETNSKIANTVASSFSNFVKPYQYLSFSNNPDFTLDISKNNNGGYTVSL
                     LGKGDDVRYTKTITDSLSIEDCKYFLEGIKRNMRVKYLRNLPDGGTLAKDIKVEIVPK
                     KATNNQNEIVMSGKDLFEIRITNTGKSDYYYTIIDIMPENEMKVLLPDETSEPQDFVI
                     QPGQTIPIGDIEVDETTPNGKEIFKIIVSKIPMDLRNVVNRTRTRSANAAMKSVESVI
                     DDLFKDSNDQRATRSSISNVKVDEVGILSCGFTVKNK"
     gene            complement(3391..5067)
                     /locus_tag="PLU36_06830"
     CDS             complement(3391..5067)
                     /locus_tag="PLU36_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46501.1"
                     /translation="MKVVKKLFFTLSFIMPALLLAQSSYLTLGGKEEWLLNRMEIKTN
                     ANGLSFSSLKPFNRKYITNTADYWDSLYTAGNKNATVLTSTDKYNLQRFLMANSEWSK
                     PKEIFKSEKPIWKYFFHNRSNMIELQNSDFVLIANPILQYEQGSKGGNSKSTFINKRG
                     INVRGLIGTKIGFHFYFTENQERLGDYVAAWKNKFIAYPGAGYIKNFKTGGYDYFDIR
                     GGLSWKVAKFMDMQLAYDRNFIGNGYRSLFLSDFSTNYMYFKVYTHFGKFQFQNIFAE
                     LVSYKKSGSDRIYPRKYFRASYLNFKATKWLNLGLFEGLMLADNSAVSLPLFNPIMFT
                     NVSGNKNDKSYIGFDVKANIAKRFQLYGQLMIDKLKTDELKNDWWGNRFAYQAGLKYT
                     DAFGIKNLDVQVETNVARPFTYAANDSVTSYNHYNQPLAHPLGANFKEYIGIIRYQPI
                     KKLYVEAKAIYYKQGLDSSILNINQNYGSNIFNFMNTRPFENGWKIGTGNLANCTLIS
                     LVASYEIKENLFIDIAYLTRNFKLKTAGITNTNILSAGIRWNIGRREMLF"
     gene            complement(5094..>6331)
                     /locus_tag="PLU36_06835"
     CDS             complement(5094..>6331)
                     /locus_tag="PLU36_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46502.1"
                     /translation="TKDKQSLYSMRVGLNATGLVAKKIAFNIYATGNRERTPLYVRAY
                     TSKFKAVPGFSNFNFTDSNAVQYFDVRASAQWTLAKFMDMQVGYDRNFIGNGIRSLFL
                     SDFSGNSLFFKSNIRLWKLNFESLYMQLTPQFGITTKANFKKYLRINTLSIDATNWLN
                     IGIFDAVVFGRKNQFELNYLLPFTFLRAMEQQSGSPDNALMGLNVKANLKNKIQLYGQ
                     VLLDEFLLGEIKANRGWWANKFGYQLGVKYLDVLNIKNLDMQVEINRARPFTYTHFDS
                     VSNYSNNNMPLAHPLGAGFQEYIVATKYQPLKKLFLDAKFIYYYQGQDTAGINFGNNI
                     LRDYKDRPTNYGWKIGSGNKATCIYFNTNIAYEVKENLFFDAGIVFRNYKLASGTTNN
                     TVIFNVGFRWNITKQQFEF"
BASE COUNT         2240 a         1035 c          925 g         2131 t
ORIGIN      
        1 cgtatttata atattatcta gtgcttgccc gtatgcttca ttccaattat catttaacga
       61 atctattgct tcggcaattt caacaaatcg tacccaatta ggataagtta atgagccatc
      121 acccgttgga gtaacaactt gagcaatatt agctacctgc aaacaaattt cgtcttttaa
      181 gatttctaag tgagactttt cttcctctat tttagcaaca acttcatctg ttaatagagg
      241 tatatctgaa gcattaatag tttgcccgct aatttcgtgt gaaacattgt tctctaatct
      301 tttgttacat gaacttactg aaagtgtaat taaaatagca taaatgagtg ccaatgaaat
      361 gtattttttc acttttctga tttttggttt aggtcaaata ggaaaatcct taacatagtt
      421 tgacttgcta caacctgcat actttttagg taagttgtcg gtgtgtacaa cctttggcat
      481 taaaataatt tcgcctttct tattcttcgt agtcatttgg taaagctctt tttcgtcctg
      541 tattgaccgt tgaagttagc aggatgtcaa aattatattg ctgccaacat aaattgttaa
      601 tcaaaacaat aattaccctt tcatacaccc aattttttac acagatattc tattccccta
      661 taagatattt tatagaaccc cattaactta ttccgatatt ttatattttt acagcatgga
      721 aacaatagga caacgcatag cacaagaacg ttttgccaaa cgttggacac aagagaaact
      781 tgcctctgaa ttaggcatta cacaaagtct tttatctgat attgaaaatg ataaagtttc
      841 gcccaaatgg caaacaatac tatctactgc cgaaaaacta gaagtccctt tttccaaatt
      901 attgcctaat gacagtaata ttttatatca accaagtatg aatgacaatt ccataggttt
      961 tgtacataat tataactcct ttgcagatga acgtaaacta tgggaagcct tactccaagc
     1021 caaagatgaa ataatacaaa gccaaaaatt ggtcatagct actttgcagg agcaaggtaa
     1081 aaagggataa tctcgtttgt tctattagct ttagctcaca gacaacagag gaaaaatgta
     1141 aaatataaac atagtttata gctaaaagct aacagttttt accaaaaaac agcgttaatc
     1201 ctaacaatca taatcatctg tgttctacaa atacccaata gccgcaaaaa ataaataaaa
     1261 tgtaaaaact aacttgcagc atggaatttg cagtggtgaa tttttacaaa aactgcatct
     1321 gttaatcaat aaaaacaaat tgtatgaaaa gtatatggat ttacctattt gttgcattag
     1381 gttttatagc agccgttttt cctcctgagc aaaagaaagt tgctttaatt attgccattg
     1441 gtgattatag cgaagcacct ggatggagaa atttaagcag catgaacgat gttcgttatg
     1501 ttaaacaagc attattaaaa gtgggttttg caacaaataa tatagatact ttaattaata
     1561 aagatgctac taaacaaggc atgattaaag ctttagacga tttgtataat aaagttggtg
     1621 aaggcgatat agtttatttt caatttagtg ggcatggaca acaaattcaa gatgataatg
     1681 gcgatgaatt agatggatat gatgaagctt taataccata cgatgcttct gcaatgtacg
     1741 acccagtaac gtatagtgga gataaacact ttagagatga tttattgggt gaaaaattaa
     1801 ataaaataag aaaaaaaata ggtcctaaag gtagtttagt tgtactagtg gatgcctgcc
     1861 atagtggaac agcaacaaga gatgcaggta taaaacgtgg tatggaaaaa ccatttttaa
     1921 ccgaccccaa atatgtacca aacattaaaa ttaatttaaa caaagaaggt gagcatggat
     1981 tattagaagg ctttacaaat ggcatgggaa atatgattgt atttagtgca agtagcccaa
     2041 accaacctaa ttatgaaatg aaagataata ataacaatgg cgtaggctct cttagttatg
     2101 cttttgcaaa atctattgca gaattacctg cagaaagcag ttaccgagtt ttatttcata
     2161 aaatgaaagc aattatacaa ggagatattc ctaaccaaat accaatggtt gaaggcgatt
     2221 tagacttaga agtttttgga ggtaaataca taccaaaacc tactataatt gctgttgatc
     2281 aaaaatttaa taatgcatct aataagttta atgataccac ttttgttatt agtattggac
     2341 aattaaacag tatttataaa ggcacaacat taaaaattta tccagttggc agtgcagaag
     2401 cttattgcga tgcagtggta acctctgtaa gctcttttca aagtatatgc aaaagcaaca
     2461 agcctttgaa aaaaagtgtt gcctatgaag ccaaagtaga agctgcaagt gcaggagact
     2521 ttgcagcaga attgatgatt caaaacaatg aaacgaatag taaaatcgct aataccgttg
     2581 catctagttt tagcaatttt gtaaagcctt atcaatattt atcttttagt aataaccccg
     2641 attttacttt agatatttcc aaaaacaaca atggtggcta cacagtaagc ttgttaggaa
     2701 aaggtgatga tgtacgatat acaaaaacta ttacagacag tttatctata gaagattgta
     2761 aatatttttt agaaggtata aaaagaaata tgagggttaa atatttaaga aatttaccag
     2821 atggtggaac ccttgctaaa gatattaaag tagaaattgt tcctaaaaaa gcaaccaata
     2881 atcaaaacga aattgtaatg agtggtaaag atttatttga aattagaatt accaatacag
     2941 gtaaatcaga ttactactat actatcattg atattatgcc agaaaatgaa atgaaagttt
     3001 tattaccaga tgaaactagt gagccacaag attttgtaat acaaccagga caaaccattc
     3061 caataggcga cattgaagtt gatgaaacta cacctaatgg aaaagaaatt tttaaaatta
     3121 ttgtaagcaa aataccaatg gatttaagaa atgttgtaaa tagaactcgt acacgtagtg
     3181 ccaacgcagc aatgaaatct gttgaaagtg taatagacga tttatttaaa gacagcaatg
     3241 atcaaagagc tactcgttca tctatcagta atgtaaaagt tgatgaagta ggaattttaa
     3301 gctgtggatt tacagtaaaa aataaatagt aatttataaa ccaaagcagt attatttggt
     3361 ttaatactgc tttatttttt tgaattgact ttaaaacaac atttcccttc taccaatatt
     3421 ccaccttata cctgccgata aaatatttgt attggttatg ccagcagttt taagtttaaa
     3481 atttctggtt aaataagcaa tatctataaa taagttttct tttatttcgt aactggcaac
     3541 taaagaaatt aaagtacagt tagccaaatt tcctgtacca attttccagc cattttcaaa
     3601 tggccttgta ttcataaaat taaaaatatt gctgccgtaa ttttgattaa tattcaatat
     3661 actactatct aaaccttgtt tgtaataaat agcttttgct tctacataca attttttaat
     3721 aggctgataa cgtataattc cgatatactc tttaaaattg gcacctaatg gatgtgccaa
     3781 tggttgatta taatgattgt aacttgtaac agaatcgtta gctgcataag taaacggacg
     3841 agcaacatta gtttctactt gtacatctaa attttttatt ccaaaagcat ctgtatattt
     3901 taaacctgcc tgataggcaa atctatttcc ccaccagtca ttttttaact catctgtttt
     3961 taatttatct atcattaatt ggccatataa ctgaaacctt ttggcaatat ttgcttttac
     4021 atcaaagcca atatacgatt tatcattttt gtttccagat acattggtaa acataattgg
     4081 attaaataat ggcaagctaa ctgcactatt atctgccaac attaatcctt caaacaaacc
     4141 taagtttaac cactttgtag ctttaaaatt taaataactt gctctaaagt atttacgagg
     4201 ataaattctg tcgctaccag attttttata agaaaccaat tcggcaaaaa tattttggaa
     4261 ttgaaatttt ccgaaatgag tatatacttt aaagtacata tagttggtac taaaatcact
     4321 taaaaacaaa ctcctgtaac cattacctat aaagtttcta tcataagcca attgcatatc
     4381 cataaacttg gcaactttcc agcttaaccc acctctaata tcaaagtaat cgtacccacc
     4441 ggttttaaaa ttttttatat aacctgctcc aggataggca ataaatttat ttttccaggc
     4501 tgcaacataa tctcctaatc tctcctgatt ttcggtaaaa taaaagtgaa acccaatttt
     4561 agtaccaatt aatccacgaa cattaatacc tcttttatta ataaaggtgc ttttagaatt
     4621 tcctccctta cttccttgtt catattgcaa tattggatta gcaattaaaa caaaatcgct
     4681 gttttgcaat tcaatcatat tagaacgatt atggaaaaag tatttccaaa ttggcttttc
     4741 gcttttaaaa atttctttgg gtttactcca ttcactatta gccattaaaa agcgttgcaa
     4801 attatattta tctgttgaag ttaaaacagt ggcattttta tttcctgctg tgtataaact
     4861 atcccaataa tcagcagtgt tagtaatata ttttctgtta aatggtttta atgatgaaaa
     4921 tgataaacca ttggcattcg tttttatttc cattctattc aataaccatt cttcttttcc
     4981 gcctaatgtt aagtaactgc tttgggccaa aagcaaagca ggcatgataa aagacaaggt
     5041 aaaaaataat tttttcacta ctttcattat taaattattt gtaaatattt ttattaaaat
     5101 tcaaattgct gtttagtaat attccaccta aaccctacat taaatattac tgtattatta
     5161 gttgtgccag aggcaagttt ataatttcta aaaacaatac cagcatcaaa aaataaattt
     5221 tcttttacct cataagctat gttggtatta aaataaatgc aagttgcttt attaccagac
     5281 ccaatcttcc agccataatt ggttggcctg tctttgtaat ctcttaaaat attattacca
     5341 aaatttatac cagccgtatc ttgcccttga tagtaataaa taaacttagc gtctaaaaaa
     5401 agttttttta aaggttggta tttggttgct acaatatact cttgaaagcc tgcacctaat
     5461 ggatgtgcta aaggcatatt attgttgcta tagttagaaa ctgaatcgaa gtgtgtataa
     5521 gtaaatggtc ttgctctatt tatttccact tgcatgtcta agtttttaat gttaagtaca
     5581 tctaaatatt ttacacccaa ttgataccca aatttatttg cccaccaacc tctgttggct
     5641 ttaatttctc ctaataaaaa ctcatctagc agcacctgcc cataaagctg aattttattt
     5701 tttaaattgg cttttacatt taagcccatt aaagcattat ctgggctacc actttgttgt
     5761 tccattgctc ttaaaaaagt aaacggaagc aaataattta attcaaactg attttttcta
     5821 ccaaaaacta ctgcatcaaa aatgccaata tttaaccaat tggttgcatc tatgcttaaa
     5881 gtgttaatac gtaaatattt tttaaaatta gctttggttg taataccaaa ctgaggggtg
     5941 agttgcatat acaaactttc aaaattcaac ttccataacc taatgtttga tttaaagaat
     6001 aaagagttac cactaaaatc acttaaaaat aaacttctaa ttccattgcc tataaaattt
     6061 ctatcgtacc caacttgcat atccataaac ttagctaatg tccattgtgc cgatgctctt
     6121 acatcaaaat attgcactgc attactatcg gtaaaattaa aattactaaa gcctggtaca
     6181 gctttaaact tgctggtata ggctcttaca tataaagggg ttctctctct attgcctgtt
     6241 gcataaatat taaaagctat ttttttagca accaatccag ttgcattcaa tccaacccgc
     6301 atactatata aactttgttt atcttttgtt t
//
LOCUS       DAOQPK010000125         6320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_7165_length_6320_cov_4.221868, whole genome shotgun sequence.
ACCESSION   DAOQPK010000125 DAOQPK010000000
VERSION     DAOQPK010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6320
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7165_length_6320_cov_4.221868"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="PLU36_06840"
     CDS             <1..957
                     /locus_tag="PLU36_06840"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HMZ46503.1"
                     /translation="YIIGPILFVWLSFSIYKQINEQPNLPDAWQAIVNSYKGAEAWKL
                     YVVILLMFVNWGLEAKKWQMLVSGIQNVSFFRAFRAIFTGQAIALNTFNGVGEYVGRV
                     IYLNEGNRLRAIALSIVGSMSQIIITLVMGLIGLLYVKFKILDETHHVQGLSKFWLDA
                     MLISLGFWTLVFLVLYFQLSWLTKLVEKIPFVAKYSFYIQKLEDFHYKELTKILLLSF
                     FRYVVFVVQYLLLLQLFNVEVVWYKLAWMVCVLFLVLAVIPSIPIAELGLRGETSKQL
                     FGLLSSNIIGIVFTAAIIWIINRVIPAIAGSLFILGVRLFKK"
     gene            963..1760
                     /locus_tag="PLU36_06845"
     CDS             963..1760
                     /locus_tag="PLU36_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HMZ46504.1"
                     /translation="MKKKVLSLFVAVGSWLTLTSADDDFCNIRNTTFVDGENITYKIF
                     YNALGIYLDAGTASFTVTKETLNNTPVFHIVGLGTSNSSYDWIFKVRDRYESFIDTTT
                     LLPIKFIRNVDEGGYKFYENVTFNQQTNSAITNKGVYKVPNCVQDVLSSIYYSRNIDF
                     NKYKIGDKIPFTMFLDNEIHNLYIKYYGKETIKTRYGKFKAIKFKPLLIKGTIFEGGE
                     KMTVWVSDDANHIPLRIESPIAVGKIKVDMMMYKNLRNSLSSLIKFR"
     gene            complement(1766..2434)
                     /locus_tag="PLU36_06850"
     CDS             complement(1766..2434)
                     /locus_tag="PLU36_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46505.1"
                     /translation="MRLLFLFFILLFINNNLNAQCATFKLARNGDTLNCVDKAGNKRG
                     KWIIKVDPLRGNPGYEEEGVFADNRKEGIWRRYNLMGDLIAIQSYKWGNLDGVSQYFT
                     ISGIEREESWKAMNPLNAYDTFLVEDINDENKYQQVILPNDGKSLKNGRWTWYRPGGM
                     GIIKTELWSLNKLVVPKTETQNTNEEEIKKPEQPKPKPKEVNDYEKKNKNKKSVKVRD
                     GKTG"
     gene            complement(2519..3229)
                     /locus_tag="PLU36_06855"
     CDS             complement(2519..3229)
                     /locus_tag="PLU36_06855"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HMZ46506.1"
                     /translation="MVKYEIIEENDFFIAINKPSGLLSVPDRKQSEPSLKDFLLQKYS
                     SIYTVHRLDKDTSGVIVFAKDADTHKQLSLLFEGRDVEKFYLGLVHGSLLHHNGSIDA
                     PIMMHPANNATMIIHQKGKPSYTDYTLLEQFKNYSWVQFQIHTGRTHQIRIHSQYMGN
                     SIVCDAIYGDDKPILLSSLKKKFKLSKKEEEEKPLLNRLALHSALLKFTFNNQAYHIE
                     APLPKDLRALLEQLRKNN"
     gene            complement(3232..4293)
                     /locus_tag="PLU36_06860"
     CDS             complement(3232..4293)
                     /locus_tag="PLU36_06860"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HMZ46507.1"
                     /translation="MKKNGFDKFINKEPKAAAKKEAFRQEKRRAKAAANALGEEMRRK
                     KQDALNNKLDVKDKFSTAKKNIKPTNKKQPTTYNKFSTTNKKDIGSKVQNTTSKYQPS
                     ANMPLNKFIAHAGICGRREAAELVKKGEITVNNEIIFEPGFKVNGDETIKFKNKRIFI
                     QQNLVYILLNKPKDYITTVKDPQGRKTVFDLIKNAAPERVYPVGRLDRNTTGVLLLTN
                     DGDLAQKLSHPSYQIKKIYEVKLDKPVLKKDMEAIANGITLEDGFIQADSIAYADDKD
                     KSIIGIEIHSGKNRIVRRIFESLGYDVRNLDRVMFANLTKKNVDRGKWRMLSEKEIRL
                     LKFMNKSFMKNNKSKASAE"
     gene            complement(4385..5689)
                     /gene="murA"
                     /locus_tag="PLU36_06865"
     CDS             complement(4385..5689)
                     /gene="murA"
                     /locus_tag="PLU36_06865"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HMZ46508.1"
                     /translation="MSVFEVYGGQKLEGAITPQGAKNEALQVISAVLLSSETVTIHNI
                     PDILDVNLLIELLHEMKVQVTKLSRHSYSFTANNLDENYLTSEEFSKKAGRLRGSVML
                     AGPMLARFKKAYIHKPGGDKIGRRRLDTHIIGFEKLGVKFDYNTNTSCFQLSTQKLTA
                     TDMLLDEPSVTGTANIIMAAVLAEGVTTIYNAACEPYIQQLCKMLINMGAKIEGVGSN
                     LLKIYGVNKLSGTEHTILPDMIETGSFIGLAAMTQSELTIKNAGVNHLGIIPEKFRQL
                     GIKLEIREDDIYIPAQDNYEITSHLDGGILTIYDHPWPGFTPDLLSIVLVVATQAIGS
                     VLVHQKMFESRLFFVDKLIDMGAQIILCDPHRATVIGLGRRNKLRGITMSSPDIRAGQ
                     ALLIAALSAEGKSIIQNIEQIDRGYQFIDERLRSIGANIIRK"
     gene            5856..>6320
                     /locus_tag="PLU36_06870"
     CDS             5856..>6320
                     /locus_tag="PLU36_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HMZ46509.1"
                     /translation="MQKGLLIIFLLLHILLSKLHTQPVFEFRGVWVASVVNIDWPSAK
                     SLTTQQQKDEFIKLLNMHQRNGMNTMIVQIRPAADALFSSPYEPWSEFLTGTQGLAPN
                     PLYDPLEFMITETHRRGMEFHAWINPYRAVFSTQSSSVASTNITKTKPDWFVT"
BASE COUNT         1954 a         1036 c          928 g         2402 t
ORIGIN      
        1 tatattattg gcccaatttt atttgtatgg ctaagcttct ctatttataa acaaattaat
       61 gagcaaccca acttgccaga tgcatggcaa gccattgtaa actcttataa aggtgcagaa
      121 gcctggaagt tgtatgtagt aattttatta atgtttgtaa actgggggtt agaggcaaag
      181 aaatggcaaa tgcttgtttc gggtattcaa aacgtttcct tctttagagc ctttagagcc
      241 atttttacag ggcaggcaat tgcattaaat acttttaatg gcgtaggcga atatgtaggc
      301 agagtaattt atttaaatga aggaaatagg ttaagagcca ttgccttatc cattgtagga
      361 agtatgagtc aaataatcat aactttagtg atgggcttaa ttggtttact atacgttaag
      421 tttaaaatat tagatgaaac acatcatgta caaggtttaa gtaagttttg gttagatgcc
      481 atgttgatat cattaggatt ttggacactg gtttttttag ttttatattt tcaattatcc
      541 tggcttacta aattagtaga gaaaattcca tttgtagcca agtatagttt ctatattcaa
      601 aagttagaag attttcatta taaggaatta acaaaaattt tactactttc tttttttaga
      661 tacgttgtat ttgttgtgca gtatttattg ttactacaat tgtttaatgt agaagttgtt
      721 tggtataagt tagcttggat ggtttgtgta ttatttttag tacttgctgt tattccatct
      781 ataccaattg cagagttagg attaagaggc gaaacaagta aacagttatt tgggttgtta
      841 agtagcaata ttattggcat agtttttact gctgccatta tatggataat caacagagtt
      901 attcctgcta ttgcaggaag cttgtttata ttaggtgtac gactatttaa gaaataagca
      961 atatgaaaaa aaaagtatta tctctttttg ttgccgtggg ctcttggctt acattaacat
     1021 ctgccgatga tgatttttgt aatattagaa ataccacttt tgtagatggt gaaaatatta
     1081 cctataaaat attttacaat gccttaggta tttatttaga tgctggtact gcaagtttta
     1141 cagtaactaa agaaacttta aacaacactc ctgtttttca tattgtaggt ttaggtactt
     1201 caaactctag ctacgattgg atttttaaag taagagaccg atatgaatct tttattgata
     1261 ccactacctt attacctata aaatttatta gaaatgtaga tgaaggtgga tacaagtttt
     1321 acgaaaatgt tacatttaat caacaaacaa attcggcaat taccaataaa ggtgtatata
     1381 aagtaccaaa ttgtgtacaa gatgttttaa gctcaattta ttattctaga aacatagatt
     1441 ttaataaata taaaattggc gataaaatac catttaccat gtttttagat aatgaaatac
     1501 acaaccttta tataaaatat tatggtaaag aaactatcaa aacccgatat ggaaagttta
     1561 aagcaataaa atttaagcca ttattaatta aaggaaccat atttgaagga ggagaaaaaa
     1621 tgactgtttg ggtaagcgat gatgctaacc atataccttt aagaatagaa agccctattg
     1681 ctgttggtaa aattaaagtg gatatgatga tgtataaaaa cttacgtaat tctttaagct
     1741 ccctaattaa gtttagataa gaatattaac cagtttttcc gtctctaacc ttaaccgatt
     1801 ttttgttctt gtttttcttt tcgtagtcgt ttacttcttt aggttttggt ttaggctgtt
     1861 caggtttttt tatttcttcc tcgttagtat tttgtgtttc tgttttagga accactaact
     1921 tatttagcga ccacaattct gttttaataa tacccatgcc tcctggtcta taccatgtcc
     1981 agcggccatt ttttaaactt ttaccatcat tgggtaatat tacttgctgg tatttatttt
     2041 catcatttat atcttctact aaaaaagtat cataagcatt taatggattc attgccttcc
     2101 agctttcttc tctttctata cctgaaatag taaaatattg cgaaacccca tctaaattcc
     2161 cccatttgta actttgaatt gcaatcaaat cacccatcaa attatatctt cgccaaatgc
     2221 cttctttcct attgtctgca aaaactcctt cttcttcata acctgggtta cctcttaatg
     2281 ggtccacttt aataatccat tttcctcttt tattgcctgc cttatctaca caatttaaag
     2341 tgtctccatt tctggcaagc ttaaaagtag cacattgagc attaaggtta ttatttataa
     2401 acaataagat aaaaaataag aataacaaac gcataccaat aattttaatt ctcaaaaata
     2461 aactatatta atattaaaaa agttataaaa tattctacca cctatataca aacatttttt
     2521 aattattttt tcgtagctgt tctagcaaag ctcttaaatc ttttggcaat ggtgcttcta
     2581 tatgataggc ttgattatta aaggtaaact ttaataatgc tgaatgtaaa gccaacctat
     2641 ttaataatgg cttctcctcc tcttcttttt tagaaagttt aaatttcttt tttaaagaag
     2701 aaagtaaaat aggtttatca tctccgtaaa tagcatcaca aactatgcta ttgcccatgt
     2761 attgagagtg aattcttatt tgatgagttc ttcctgtatg aatttgaaat tgaacccatg
     2821 agtagttctt aaactgttct aataaagtat aatctgtata agagggcttt cctttttggt
     2881 gaataatcat agttgcatta ttagcaggat gcatcataat gggagcatca atactaccat
     2941 tatgatgtaa caaactacca tgtaccaagc caagataaaa tttttcaaca tctctgcctt
     3001 caaatagcag agatagttgt ttgtgggtat ctgcatcttt agcaaaaaca attactccac
     3061 tagtgtcttt atctaaccta tgtactgtat aaattgaaga atatttctgt agcaaaaaat
     3121 cctttaaact aggttccgat tgttttctat ctggtacgct taataagcct gatggtttat
     3181 ttatagcaat aaaaaaatcg ttctcttcta ttatttcata tttaaccata gctactctgc
     3241 agatgctttg cttttattgt ttttcataaa acttttattc ataaacttca gtaaccttat
     3301 ttctttttca ctaagcatac gccacttgcc tctatctaca ttttttttag ttaggttggc
     3361 aaacataact ctatctaaat ttcttacatc atagcctaaa ctctcaaaaa ttcttcttac
     3421 aattctattt ttgccgctat gtatttcaat tccaataatg cttttatctt tatcatcggc
     3481 ataagcaata ctatctgctt gtataaagcc atcttctagt gtaataccat ttgcaatagc
     3541 ttccatatct tttttaagta caggcttatc aagctttacc tcataaattt tctttatttg
     3601 gtaagatggg tggctaagtt tttgtgctaa atccccatcg ttagtaagta ataaaacacc
     3661 agttgtattt ctgtctaatc tgcccacagg atataccctt tctggggcag cattttttat
     3721 taaatcaaaa accgtttttc tgccttgtgg gtccttaaca gtagtaatat aatcttttgg
     3781 tttattgagt aaaatatata caagattctg ctgtataaaa atacgtttgt ttttaaattt
     3841 tatagtttca tctccattta ctttaaagcc tggctcaaaa attatttcat tgtttacagt
     3901 aatttctcct ttttttacta actctgcagc ttctcttctt ccgcaaatac ctgcatgagc
     3961 aataaattta tttaaaggca tattggcaga tggctgatat tttgaagtag tgttttgaac
     4021 tttgctgcct atgtcttttt tattggttgt agaaaatttg ttgtaagttg ttggttgttt
     4081 tttattggtt ggctttatat tcttttttgc tgtagagaat ttatctttta catccaactt
     4141 attatttaat gcatcttgtt ttttccttct catctcttct cctaatgcat tggctgctgc
     4201 ttttgctctt cttttttctt gacgaaatgc ctctttttta gcggcagctt taggttcctt
     4261 attgataaat ttatcaaaac catttttttt catactatac aattttactg ttttacaaca
     4321 ggatgattaa tgttgcaaag atatttatta gtattattat atgcttaatt aaaaagtgta
     4381 gtagttattt tctaataata ttagctccaa tgcttctcaa tctttcatca ataaattggt
     4441 atcctctatc tatttgttct atgttttgaa taatactttt tccctctgca cttaaagctg
     4501 caattagtaa tgcttgtcct gctctaatat ctggactgct cattgttata ccacgcagtt
     4561 tatttcttct tcctaagcca ataactgtag ctcggtgtgg atcgcataaa ataatttgtg
     4621 cccccatatc aattaattta tctacaaaaa acaatctgct ttcaaacatt ttttggtgta
     4681 ccaatacact acctattgct tgtgtggcaa ctactaaaac aatactcaat aaatctggtg
     4741 taaaaccagg ccatggatgg tcataaatgg ttaaaatacc accatctaaa tgtgaggtta
     4801 tttcgtaatt atcttgtgca ggaatataaa tatcatcttc tcttatttca agtttaatac
     4861 ctaattgtct aaatttttct ggtataattc ctaaatggtt aacgccagca tttttaatag
     4921 ttaattcact ttgtgtcata gcagcaaggc caataaagct gccagtttca atcatatctg
     4981 gtaaaatggt atgctctgtt ccggataatt tatttacacc ataaattttt aataaattac
     5041 ttcctacacc ttcaattttt gcacccatat tgatcaacat tttgcatagc tgttgtatat
     5101 aaggttcgca agctgcattg taaattgttg taacaccttc tgccagcact gcagccatta
     5161 taatatttgc agttccggta acacttggct catctaacaa catatcggtt gcagtaagct
     5221 tttgggtaga tagttgaaag caactagtat ttgtattata atcaaacttt acacctaatt
     5281 tttcaaatcc tataatgtgt gtatccaatc ttcttcttcc aattttatct ccaccaggtt
     5341 tatgaatata ggctttctta aaccttgcaa gcattggtcc agcaagcatt acactgcctc
     5401 gtaatctccc tgctttttta ctaaattctt cactagttaa ataattttca tctaagttat
     5461 ttgctgtaaa agagtaacta tgcctactta atttagtaac ctgcaccttc atttcatgca
     5521 ataactctat taaaagattt acatctaaaa tatctggaat attatggatg gttacggttt
     5581 cactggataa taaaactgca ctaattactt gtagggcttc atttttggca ccttgtgggg
     5641 ttattgctcc ttcaagtttc tgtcctccat atacttcaaa aacactcatg gttatttact
     5701 aaaagtttta atattaaagt aaaagtatta tgttgtaggc acaaattata aaattatttg
     5761 tcctcctaaa aaaattaaga agagttgtta aatttttaat tcaaattttt atttgcataa
     5821 cagtaattat cttcggctag tttaaaaata aattcatgca aaaaggtttg ctaatcatat
     5881 ttctgctatt gcatattttg ctaagtaagt tacatactca acccgtattt gaatttagag
     5941 gagtttgggt agcaagtgta gttaatatag attggccaag tgctaaaagt cttacaactc
     6001 aacaacaaaa agatgaattt attaagttgt taaatatgca ccagcgtaac ggaatgaata
     6061 caatgattgt acaaatacgt cctgctgcag atgcactttt ttcttctcct tacgaacctt
     6121 ggagtgagtt tttaacaggc acgcaaggct tagcaccaaa tcctttatac gatcctttag
     6181 agtttatgat tactgaaaca cacaggcgtg gaatggagtt tcatgcttgg ataaacccat
     6241 atagagccgt ttttagtact caatcatctt ctgttgcttc aactaatatt actaaaacaa
     6301 aaccagattg gtttgttacc
//
LOCUS       DAOQPK010000126         6319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_7167_length_6319_cov_5.193487, whole genome shotgun sequence.
ACCESSION   DAOQPK010000126 DAOQPK010000000
VERSION     DAOQPK010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6319
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7167_length_6319_cov_5.193487"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..1184)
                     /locus_tag="PLU36_06875"
     CDS             complement(78..1184)
                     /locus_tag="PLU36_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HMZ46510.1"
                     /translation="MIMEDILLIQTIERYLKGKMLPEEKTIFEQQRKNIPEIDQMFVE
                     HIMFLNQLEDFAGNQRLKQSLHNVHNQLAKDGIINEGAATTTKAKVIQLWNRYRKVTA
                     IAAVVAGAMALLTSGLVAFFAPVNKLAIQELGQEVEKLKQGQRYQVNKIHEVESKIPK
                     DAEVRGGGSGFLIDNKGYLVTNAHIFKGATFANVYNNGKEYKTKILFIDSEKDLAILK
                     IEDKDFAPLKSLPYGISKQGIDLGEEVFTLGYPKNEIVYNMGYLSAKTGYAGDTLTCQ
                     IQMSANPGNSGGPLLNKKGEIIGVISTRQRQAEGVTFAITAKYIYGLVNDLKKSDVAY
                     QNIKLPTYSSIKGMDRVGQIKKIEDCVFLIKAYN"
     gene            complement(1181..1747)
                     /locus_tag="PLU36_06880"
     CDS             complement(1181..1747)
                     /locus_tag="PLU36_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ46511.1"
                     /translation="MKAELNEELLLKGLAENNSKAIETIYKQNFNIVQAFILNNNGSY
                     DEARDIFQEAMITLYEKVQTESFVLTCKINTYVYSVCRRLWLKRLQQLGRYSNQIDGF
                     EETLQVEDEIEAHERKNTDFAMMEKALNSIGEPCRTLLESFYIKKIGMQELAKQFGYT
                     NADNAKTQKYKCLLRLKKIFFAQYKMGE"
     gene            1896..2081
                     /locus_tag="PLU36_06885"
     CDS             1896..2081
                     /locus_tag="PLU36_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46512.1"
                     /translation="MKKVLALLAIVGFVACNSAENTEVKTDSVAPATPAVDTTTKAVD
                     TTKAVDTTKAAATTPAK"
     gene            2255..2599
                     /locus_tag="PLU36_06890"
     CDS             2255..2599
                     /locus_tag="PLU36_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46513.1"
                     /translation="MLEKREEDFITFWEKNRLIQKKNTKQFILGLTFGLFIGLSTLML
                     IFSGWYQRATMVATTRLNPFVFIVIIVIIAVFMAYLNRKFKWERHEQQYLELLAKKQK
                     LKEKHTANQEGI"
     gene            2754..5837
                     /gene="secDF"
                     /locus_tag="PLU36_06895"
     CDS             2754..5837
                     /gene="secDF"
                     /locus_tag="PLU36_06895"
                     /inference="COORDINATES: protein motif:HMM:NF009585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HMZ46514.1"
                     /translation="MQLKGLVRFFAIALTLICLYQLSFTWIVHNHENKMEAKAKKWIK
                     DTYASPEAKYSGNVEAKALYEDTLQQLTKVRLQRLLDSTKESKIGPFGLTTYQNAKDK
                     ELLLGLDLQGGMSVTMEVGLDGLIKSLSGYTKDAQFNTAIESAVKLKANSSADLVSLF
                     VQEYKKINPSGKLAPFFATKSSGKIKFDDSDENVVKYLKEQSDNAFANTFRIVNKRID
                     KFGVASPNINQNPAKGIITVELAGVTDHERVRRYLQSTANLQFFEVYTLQDNELSKGF
                     EEANKALQNYLNGVQTVVDTTKAVTTPNTTIDTTQTVKISDVTGTETTKDTSLNKKNV
                     DPIKSLLFGGREHQPTQNRKGGYSYPAALAYVALKDTGLLNQYLALDIVKTKFPSNLV
                     FMYGKAEDENGKVSKTNLNLYAIKTLDNGQAKIEGEHVAEAKQGYDGSRPIIHMNMTK
                     EGTKLWGDLTTKNVDKPIAVVLDNFVYSAPNVNEPITTGSSQISGNYTVKEAQDMADI
                     LQTGKLPAPAKIVQEQIIGPTLGAEAVKGGGMSFLVSFLIIFALMLIYYNTGGWVANI
                     ALVLNLLFTIGILAALGFTLTSAGIAGLVLTIGMAVDTNVIIFERIKEELTKGKSYTV
                     AVNDGYKRSLAPVLDSHVTSFLTAAILFNFGFGPILGFATTQMLGLSLSLFCGILVSR
                     LITDIYASSKKDRHFQYFTKISSRIFKHATFPFVKYRKIAYVISVVILVLGIASFFNG
                     FDQGVEFAGGRSYVIKFKQNVSIDNLRSELKDVFEGETPIIKTVGTADQLDITTSYKI
                     HETGQKVDSLVEQTLFGGLKKYLPQGTTYYEFATQYKQRSQTVLPTISKDLKEGAVKA
                     ALVAVIMICLYIFLRFRDWRYSFGTIVSLMHDVFVTLIVFSFAKSIVPFPLEIDQHFI
                     AAILTVIGFSMNDTVIVYDRIREDSKLYPSMGKEELVNTAINQTLSRTIMTSLTVFIT
                     ILVLFIFGGEVTRGFSFAMLIGVITGTYSSIFVAAPILIDIAKNKALKRKEEKKA"
     gene            complement(5927..6103)
                     /locus_tag="PLU36_06900"
     CDS             complement(5927..6103)
                     /locus_tag="PLU36_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46515.1"
                     /translation="MAATTTIRKLTPKQKLIAQLQKAGGIEANRKTLKALKAVFKNTN
                     LKAETLRKAAWRMK"
BASE COUNT         2109 a          959 c          992 g         2259 t
ORIGIN      
        1 agttggggta ccccaacttt atgaagactt acacagttta taagatagta ccttgcagcc
       61 cttttttgta taatttatta attgtaagcc ttaattaaga aaacacaatc ttctattttt
      121 ttaatttgtc caactctgtc cattcctttg atagaagaat aagtaggaag ttttatgttt
      181 tgataagcaa catcagactt ttttaaatca ttaactaagc cataaatata tttagcagta
      241 atagcaaaag taaccccttc tgcttggcgt tgccttgtac taattacacc aattatttct
      301 ccttttttat ttaataaagg gccaccacta ttaccagggt tggcactcat ttgaatttga
      361 caagttaggg tatcgcctgc ataaccagtt ttggcactta aataacccat attgtacacg
      421 atttcatttt taggataacc aagtgtaaat acttcttcac ctaaatcaat tccttgtttt
      481 gagataccat aaggtaaaga ttttaatggt gcaaaatctt tatcctctat ttttaaaatg
      541 gctagatctt tttcagaatc tataaataaa attttagttt tatattcctt tccattgttg
      601 tacacattgg caaaagtagc acctttaaaa atgtgagcat tggtaacaag atatcctttg
      661 ttatcaatta aaaaaccaga tccgccacct cttacttctg catctttagg aattttagat
      721 tccacttcgt gtattttatt aacctggtaa cgctgccctt gttttaactt ttcaacttct
      781 tgccctaatt cttgtatagc caatttgttt acaggtgcaa aaaatgcaac taaaccacta
      841 gttagtaaag ccattgcacc agcaaccaca gcagcaatcg cagttacttt tctgtatcta
      901 ttccataatt gaataacttt agcttttgta gttgttgcag ccccttcatt aataattcca
      961 tcttttgcaa gttgattgtg tacgttatga aggctttgtt ttaatctttg attgcccgca
     1021 aaatcttcca attggtttaa aaacataata tgttctacaa acatttggtc aatttccgga
     1081 atattttttc gttgttgttc aaaaatagtt ttctcttcgg gtagcatttt acctttaaga
     1141 taacgttcta ttgtttgaat taataaaata tcttccatta tcattctccc attttatatt
     1201 gagcaaaaaa tatttttttc aatcttagta agcatttgta tttctgtgtt tttgcattat
     1261 ctgcattagt ataaccaaat tgcttagcta attcttgcat tcctattttt tttatataaa
     1321 aactttctaa tagggttctg caaggttctc caatactatt caaggctttt tccatcattg
     1381 caaaatctgt attctttctt tcgtgagcct ctatttcatc ttcaacttgt aaagtttctt
     1441 caaagccatc tatttgattg ctgtatctgc ctaattgttg caatcttttt aaccacaatc
     1501 ttctacaaac cgagtacaca taagtattta ttttgcaggt taaaacaaaa gattcagttt
     1561 gcactttttc atataaagta atcattgctt cctgaaaaat atctcttgcc tcatcgtaac
     1621 ttccattgtt atttaatata aaagcctgaa ctatattaaa attttgttta tatatagttt
     1681 ctatagcttt gctattattc tctgccaatc ctttcaataa aagttcttcg tttaactctg
     1741 ctttcactat tgtgtgttat ttatacattt taatgattaa aagtaacccg ccaaacgagt
     1801 aaaaaaaaat atttaaattt ttttaaaaaa gttgggttac cttttttcaa agttgggtat
     1861 taacatgaaa tttaactcat aaaaaaacaa aaatcatgaa aaaagtactt gcattattag
     1921 caatcgtagg tttcgtagct tgtaactcag ctgaaaacac agaagtaaaa actgatagcg
     1981 ttgctccagc aactcctgca gtagatacta caactaaagc tgttgataca actaaagctg
     2041 ttgatacaac taaagctgct gctactactc ctgctaaata attaccttat ttagacgaaa
     2101 tgtcttgatt ttgtgcaagt gatatagaaa agtgcagttg acttgcaact gagggcacaa
     2161 aaaataaata cgtttcatat ggcaagcaat cgttaaaagc cgtcttgttt ttacaaggtg
     2221 gcttttttat ttcgtttctt ttaattttgc ctgtatgtta gaaaaacgag aagaagattt
     2281 tattaccttt tgggagaaga ataggttaat tcagaaaaaa aataccaaac aatttattct
     2341 aggcttaaca tttggtttat ttataggctt atctacttta atgcttattt tttctggctg
     2401 gtatcaaaga gctacaatgg tggctacaac aaggttaaac ccttttgttt ttattgtaat
     2461 tattgtaata attgctgttt ttatggctta tttaaaccga aagtttaaat gggagagaca
     2521 tgaacaacag tatttagaac ttttagcaaa aaaacaaaaa ttaaaagaaa aacatactgc
     2581 taaccaagaa ggcatttaaa tagatatttt cccattttat actcaatttt taataaagga
     2641 aagtggaata agtaaataac aaattttaaa tattgatttt tttgctgttt tttacatata
     2701 tcattcccct atctttgccc actctaaaaa aaatgtaaaa cccgttactt attatgcaac
     2761 tcaaaggttt agttagattt tttgctattg cacttacttt aatttgtttg tatcaacttt
     2821 catttacatg gatagttcat aaccatgaaa ataaaatgga ggcaaaagca aaaaaatgga
     2881 taaaagacac ttatgcatcg ccagaagcta agtatagtgg taatgttgaa gcaaaagctt
     2941 tgtatgaaga tacacttcaa caattaacca aagttcgttt acaacgttta ttagatagta
     3001 ccaaagaatc taaaattggt ccatttggtt taactactta tcaaaatgca aaagacaaag
     3061 aacttttatt aggtttggat ttgcaaggtg gtatgagtgt aacaatggaa gttggtttag
     3121 atggattaat aaaatcatta tctggctata ctaaagatgc acagtttaat actgcaatag
     3181 aaagtgctgt taaattaaaa gccaatagca gtgccgattt agtttcgctt tttgttcaag
     3241 agtataaaaa gataaaccct agtggtaaat tagctccatt ctttgcaact aaaagtagcg
     3301 gtaaaattaa gtttgatgat agcgacgaaa atgttgttaa atatttaaaa gaacaatcag
     3361 acaatgcttt tgctaataca tttagaatag taaataaacg tattgataaa tttggagtag
     3421 catctcctaa tatcaatcaa aatcctgcaa aaggaattat tactgtagaa ttagctggtg
     3481 ttactgacca tgaaagagtt agaagatact tacaatctac agctaacctt cagttttttg
     3541 aagtatatac acttcaagat aacgaattaa gtaaaggctt tgaggaagct aataaagctc
     3601 ttcaaaatta tttaaatggt gtacaaactg tagtagatac aactaaagca gttacaacac
     3661 caaatacaac tattgataca acacaaactg taaaaatttc tgatgtaaca ggaactgaaa
     3721 caacaaaaga cacttcctta aataagaaaa atgtagatcc aattaagtcg ttgttgtttg
     3781 gaggtagaga gcatcaacca actcaaaaca gaaaaggggg ctactcttat cctgcagctt
     3841 tagcttatgt tgcattgaag gatacaggtt tattaaacca atatttagct ttagatattg
     3901 taaaaactaa attcccaagc aatttagttt tcatgtacgg caaagcagaa gatgaaaatg
     3961 gcaaagtatc taaaacgaat ttgaatttat atgctattaa aactttagat aatggccaag
     4021 caaaaataga aggtgaacat gtagcagaag ctaaacaagg ttatgatggt agtcgtccta
     4081 ttattcatat gaatatgact aaagaaggta ctaaactttg gggcgaccta acaacaaaaa
     4141 atgtagataa accaatagca gttgtattag ataattttgt ttattctgct ccaaatgtaa
     4201 atgaaccaat tacaactggt agctctcaaa tttctggaaa ttatactgta aaagaagcac
     4261 aggatatggc agatatttta caaactggta aattacctgc acctgctaaa attgtacaag
     4321 aacaaattat tggtcctact ttaggtgctg aagcagttaa aggtggcgga atgagtttct
     4381 tggtttcttt tctaatcatt tttgctttaa tgttaattta ctataatact ggtggttggg
     4441 tagccaatat tgctttagtg ttaaacttat tatttacaat tggcatttta gctgcattag
     4501 gttttaccct tacatctgca ggtattgcag gtttggtact aactattggt atggcggttg
     4561 atactaacgt aattattttt gaacgtataa aagaagaact tacaaaaggc aaaagttata
     4621 cagttgcagt aaacgatgga tacaaacgtt ctttagctcc tgtattagac tctcacgtta
     4681 catctttttt aactgcagct atattattta attttggttt tggaccaata cttggttttg
     4741 ctacaacaca aatgttgggg ctttcattaa gtttattctg cggtatttta gtttctcgtt
     4801 taattacaga tatttatgca agcagtaaaa aggatagaca ctttcaatat tttactaaaa
     4861 tttcaagccg tatttttaaa catgcaacct tcccatttgt aaaatacaga aagatagctt
     4921 atgtaatttc tgttgttatt ttagttttag gcattgcatc cttctttaat ggttttgacc
     4981 aaggtgtgga atttgcagga ggaaggagct atgtaattaa atttaaacaa aatgtttcta
     5041 tagataattt aagatctgaa ttgaaagatg tttttgaagg agaaactcca ataattaaaa
     5101 ctgttggaac agcagatcaa ttagatatta caacttctta taaaattcat gaaacagggc
     5161 aaaaagtgga ctctttagtt gaacaaactt tatttggtgg attgaaaaaa tatttaccac
     5221 aaggaaccac ttattatgag tttgctacac agtacaaaca acgttctcaa actgtgttac
     5281 ctaccatttc taaagactta aaagaaggtg ctgtaaaagc tgccttggtt gcagtaataa
     5341 tgatttgcct ttacatcttt ttaagattta gagactggag atactcattt ggtaccattg
     5401 taagtttaat gcacgacgtt tttgtaactt taatagtatt ctcctttgca aaaagtattg
     5461 ttccttttcc gttagaaata gatcaacatt ttattgcagc cattcttaca gttattgggt
     5521 tttcaatgaa tgatactgta attgtgtatg accgtattcg tgaagattct aaactatacc
     5581 caagtatggg taaagaagag ctagtgaata cagctattaa ccaaacatta agtcgtacta
     5641 ttatgacttc gttaactgta tttatcacca ttttagtatt gtttattttt ggtggagagg
     5701 taacaagagg tttctctttt gcaatgttaa taggggttat tactggtact tattcttcta
     5761 tttttgttgc agcaccaatt ttaattgata ttgctaaaaa caaagcatta aaaagaaaag
     5821 aagaaaagaa agcgtaagca acttttcgta atataagata atactaaagc cccaaatatt
     5881 tggggcttta tttatgagga tattttaaaa tagggattta ataaaattat ttcattctcc
     5941 atgctgcctt tcttaaagtt tctgctttta aattagtgtt tttaaaaact gcttttaatg
     6001 cttttaatgt ttttctgttt gcttctatac cacctgcttt ttgtaattgt gcaattaatt
     6061 tttgcttagg tgtaagtttt ctgattgttg tagttgctgc cataaaaaac gtttttacaa
     6121 gtataagttt tttgttggtg taagaggtaa gggtagaaat tataaccaca tagaaatttt
     6181 gtaaggcagc agttttaagc ctttgtatgc tgtaaaatct ttagagtttt ctgtaagtag
     6241 tgggatttta tttgcaaagg caatagatgc aattaaaaaa tctttacttt gcctgttgct
     6301 taccctgtat ttcatagca
//
LOCUS       DAOQPK010000127         6297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_7208_length_6297_cov_4.269305, whole genome shotgun sequence.
ACCESSION   DAOQPK010000127 DAOQPK010000000
VERSION     DAOQPK010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7208_length_6297_cov_4.269305"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(637..1497)
                     /locus_tag="PLU36_06905"
     CDS             complement(637..1497)
                     /locus_tag="PLU36_06905"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HMZ46516.1"
                     /translation="MKTTLLSILTLMLFAEATAQIPKEKTLLWKIEGNSINQPSYLFG
                     TFHMLCSNDFEMPDTIKSLMGMCKQLFLEIDIDDPQMTTTMMKHIKMKDGKRLKDFLT
                     QEEYDTATVLFKKVVGMNLKMVETYKPFMLTSMLYPKMMGCNIIAFEKELAKLAKNND
                     YETKGLETIEDQLAVFDDVSYKIQAKMFLKSLLEIEEGKTQIQKIVTLYNSKNIRAMQ
                     KAVKSDADLGKHEKVLLHKRNQNWIPKIENEISKLPTFFAVGAGHLAGKKGVIYLLRK
                     KGYTVTPIFY"
     gene            complement(1575..2921)
                     /gene="purB"
                     /locus_tag="PLU36_06910"
     CDS             complement(1575..2921)
                     /gene="purB"
                     /locus_tag="PLU36_06910"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HMZ46517.1"
                     /translation="MELNNLTAISPIDGRYRKQLAFLDDYFSEFALIKYRVAIEIDYF
                     LFLAEKGVFKINSTNKQRLLKVKENFSIEDANKIKNIEAITNHDVKAVEYFIKEELDK
                     ENLGNLKEWVHFGLTSQDINNTAIPLSWKHCAENEYLPMIANTHNLLVRMARKWKSVT
                     MLARTHGQPASPTILGKEIMVFAERIEGQIMQFSYIPFSAKFGGATGNFNAHVVAFPN
                     KKWIAFGNEFVDKILGLQRMQFTTQIEHYDNLAAHLDCIKRINTILIDLCRDIWTYIS
                     LDYFKQKTKKGEVGSSAMPHKVNPIDFENAEGNLGVANALLEHLSAKLPISRLQRDLT
                     DSTVLRNLGVPIAHTLLAIKSIEKGLGKLLLNENKINADLDNNWAVVAEAIQTILRKE
                     NYPNPYEALKELTRGKSSINQQSIHLFIDSLKINLELKKELKKITPYNYTGVQANY"
     gene            2997..3734
                     /locus_tag="PLU36_06915"
     CDS             2997..3734
                     /locus_tag="PLU36_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HMZ46518.1"
                     /translation="MNKIKIGAVSYLNTKPLLYGIKHSAIINNIELIEDHPANIAELL
                     LANKIDIGLVPTAIIPQISNATIVADYCIGANATVASVCLFSETTIDNIEAVILDYQS
                     RTSVALCKILLKEYWKREVKFIEGREGYRNEIKGTTAAIVIGDRAFEQAKQSAYKYDL
                     AEAWINFTKLPFVFAAWVANTNIEQEFMNAFNKANAYGIENIDKVVEDINYPFYNLQY
                     YYTNNISYFLDEEKKKGLELFLQFLKQ"
     gene            complement(3841..3912)
                     /locus_tag="PLU36_06920"
     tRNA            complement(3841..3912)
                     /locus_tag="PLU36_06920"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3877..3879),aa:Glu,seq:ttc)
     gene            4099..4692
                     /locus_tag="PLU36_06925"
     CDS             4099..4692
                     /locus_tag="PLU36_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HMZ46519.1"
                     /translation="MRTNQLSLLVIGGIILLLGGWSCSKYNGLVKTDVEVQKVWGNVE
                     TNYQRRTDLYSSVIKTIEGSANFEKSTLKDVINARAAATQVKVDINDPESLAKYQAAQ
                     GQLQSAFGRLMAVAEAYPDLKTTKSFQDFQAQIEGTENRINVARQDYNKAVQNYNLKV
                     RTFPNNIFAGFFGFKSKEFYKADPGTEKAPEVNFNIK"
     gene            4701..5162
                     /locus_tag="PLU36_06930"
     CDS             4701..5162
                     /locus_tag="PLU36_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HMZ46520.1"
                     /translation="MLPFFKKKPTNYFSEPEKQLIVNAIKQAETNTSGEIRVFIENKC
                     SYVDAIDRAKELFEKLKMFETEQRNGVLLYVALKDRQLAIFGDKGIYEKTGVEFWNNE
                     VNELLLFFNKLNYAEGIANVVIQIGNALKLHFPFNKSSDVNELKDEIVFGK"
     gene            5202..5984
                     /locus_tag="PLU36_06935"
     CDS             5202..5984
                     /locus_tag="PLU36_06935"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HMZ46521.1"
                     /translation="MKYFFSILFSLLVNISFAQKSIPKPNPPKLVNDYAGILDKHDAD
                     LLEKKLVALDDSTSNQIVVLTVKSLNGEILEDVAVNTFREWGIGNKKTNNGVLILIAL
                     EDKKVRIEVGYGLEGAIPDVIANDILKTYLAPAFKQGNYLDGINKAVDNITKAAAGEY
                     KVKRKKSNETSGKSLIIFIIILFVVLMIVGNSGRGGGGGNIMNRGGSNDIATNLFLLS
                     LLNSRSGRGNSGGGWGGGDWGGGGFGGFGGGSSGGGGASGGW"
     gene            6088..>6297
                     /locus_tag="PLU36_06940"
     CDS             6088..>6297
                     /locus_tag="PLU36_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HMZ46522.1"
                     /translation="MGRIFEVRKATMFARWDRMAKQFTRIGKEIAIAVKAGGTDPHTN
                     PALRRCMQNAKSVNMPKDRVEAAIKR"
BASE COUNT         2236 a          860 c         1031 g         2170 t
ORIGIN      
        1 taagtttacc agtaatggca caggataatt ttccagatcc tgaagatgat atacctgtag
       61 atggtggttt aactttattg ttagcagcag gagctggttt aggagcaaag aaattaagag
      121 ataacagaaa gaagcaacag aataaaaact aagcagtctg tttatcaact actacaatta
      181 ctgtaatttt aattaattac aatataacct cattaaataa taacaaaagt aataaaaagc
      241 ccttgcataa atgcaagggc tttttgatag agtaacatga aataaaatga gccgaggtag
      301 aaacctcagc ccttatttaa tccgtaccct atgaaaacaa gtttatatga gtttagctat
      361 tacaacaagc taaaaattaa tttataattt taatgtattt tgctaaaaat tcagtgaagc
      421 gtttgtggaa ttacaataca atatgtaaga ttgtacattg gcaacattgg attttaattt
      481 aaacaaaatt ggtttttata attacttata ttggtagaaa aaagtaatta ctacataaaa
      541 acgcattagt ataaatttta gtatataatt ctaattttta ttttttaaaa aatatttttt
      601 gtttaaactt ttaagaaaaa ttctaagaat taaattctaa taaaaaatag gagtaacagt
      661 atagcccttc tttcttaaca agtaaataac accttttttg ccagctaaat gacctgctcc
      721 aacagcaaaa aaagtaggta attttgaaat ttcattttca attttaggaa tccaattttg
      781 atttctttta tgtaatagta ctttttcatg tttacctaaa tctgcatcgc tttttacagc
      841 tttttgcatt gctctaatat ttttagaatt atacaaggta acgatttttt gtatttgtgt
      901 ttttccttct tctatttcta ataaagattt taaaaacatt ttagcttgta ttttataaga
      961 tacatcatca aaaactgcta actggtcttc aatagtttcc aatccctttg tttcatagtc
     1021 attgtttttt gcaagcttag ctaattcctt ttcaaaagca attatattac aacccatcat
     1081 tttggggtaa agcatagaag taagcataaa aggtttatag gtttcaacca ttttcaaatt
     1141 cataccaaca acttttttaa atagtactgt agcggtatca tattcttctt gtgttaaaaa
     1201 atcttttagt ctttttccat ctttcatttt aatatgcttc atcattgtag ttgtcatttg
     1261 cggatcgtct atatctattt ctaagaataa ttgtttacac attcccatta aagatttgat
     1321 ggtatctggc atttcaaaat cattagaaca taacatgtga aaagtaccaa ataagtagga
     1381 gggttggtta atactattac cctcaatttt ccataataat gttttctctt ttggaatttg
     1441 agctgttgct tctgcaaaaa gcatcaatgt taaaattgat agtaaagtgg ttttcattgc
     1501 tgtgttttaa gttagatata agttatagag tattctacaa ataaactatt tagaaataaa
     1561 agaaatagtg ttttctaata atttgcctga acccctgtgt agttatatgg tgtaattttt
     1621 tttagttctt tttttagctc caaatttatt tttagagagt ctataaataa gtgaattgac
     1681 tgttggttaa tactactttt acctctggtt aattctttta aagcttcata agggttaggg
     1741 taattttcct tccttaatat tgtttgaata gcttctgcaa ctactgccca attattgtct
     1801 aaatcggcat taattttatt ttcattcaat agcaacttac ctaaaccttt ttcaatggat
     1861 tttattgcaa gtagggtatg agcaattggc acacccaagt ttctaagtac tgtactatcc
     1921 gttaaatctc tttgtaatct gctaataggt aatttggctg acaggtgttc taacaaagca
     1981 ttagcaactc ctaaatttcc ctctgcattt tcaaaatcta tcggatttac tttgtgtggc
     2041 attgcactag agcctacttc tcctttcttt gttttttgtt taaaataatc aaggctaata
     2101 taagtccata tatctctaca caaatcaata agaatagtat ttattctttt tatacaatct
     2161 aaatgtgcag ctaagttatc ataatgttct atttgcgttg taaactgcat tcgttgtagc
     2221 ccaagaattt tatctacaaa ttcatttcca aatgcaatcc attttttgtt tggaaaagca
     2281 acaacatgtg cattaaaatt tcctgttgct ccaccaaatt ttgcactaaa aggtatataa
     2341 gaaaattgca ttatttgtcc ttctattctt tcagcaaaaa ccattatttc tttacctaaa
     2401 atggtaggag aagcaggttg gccatgtgta cgtgcaagca tggtaactga tttccatttt
     2461 ctggccattc ttactaataa attatgtgta tttgcaatca ttggtaaata ctcattttct
     2521 gcacaatgtt tccagcttag tggtatagca gtattgttaa tatcctgact ggttaaacca
     2581 aagtgaaccc actcttttaa attacctaaa ttttctttat ccagttcttc tttaataaaa
     2641 tattcaacag cttttacatc atggttggta attgcttcta tatttttaat cttgttagca
     2701 tcttcaatac taaagttttc tttaactttt aataagcgtt gtttgttggt tgaattaatc
     2761 ttaaaaacac ccttttctgc taaaaacaaa aaataatcta tttcaattgc tactctatat
     2821 ttaattaaag caaactccga aaaatagtca tctaaaaatg caagttgttt tctgtatctg
     2881 ccatcaatag gagatatagc agtaaggtta tttaattcca tgttgatttt tatataagtt
     2941 gaagtatagg gatatatata gaaaaaacat tttctttgta tgcaaattta ataggattga
     3001 ataagataaa aataggagca gtaagttatt taaacacaaa gcctttatta tatggaatta
     3061 aacattctgc aataattaat aatattgaac taatagaaga ccatcctgca aatattgctg
     3121 agttgttatt agctaataaa attgatatag ggttggtgcc aacagctatt ataccacaaa
     3181 ttagcaatgc tacaattgtt gctgattact gcattggagc aaatgctact gtagcaagtg
     3241 tttgtttgtt ttctgaaact actattgata atattgaagc agtaatctta gattaccaaa
     3301 gcagaacatc tgtagcatta tgtaaaattt tacttaaaga gtattggaag agagaggtga
     3361 aatttattga agggagagaa ggctatagaa atgaaataaa aggaactact gctgcaatag
     3421 ttattggtga tagagccttt gaacaagcaa agcaatctgc ctataagtat gatttagcag
     3481 aggcctggat aaattttact aaactgccat ttgtatttgc tgcatgggtg gcaaatacaa
     3541 atattgaaca agagtttatg aatgctttta acaaagccaa tgcttacggt atagaaaata
     3601 tagataaagt agttgaagac attaattatc ctttttacaa cttacaatat tactatacta
     3661 acaatatcag ctatttttta gatgaagaaa agaaaaaagg gttagaacta tttctgcaat
     3721 ttttaaaaca ataatggttt aatattttac ctatggttgt tttaatgcat tccagaaaaa
     3781 acaataagtg attttttgaa aatataaaag ccatctgcaa gtgcaaatgg cttttattag
     3841 tagcccgtag gggaatcgaa cccctctttc ctccgtgaaa gggagatgtc ctagccgata
     3901 gacgaacggg ccatattttt tgtcaatcgg gctgcaaata tagaagcaca aaaattagta
     3961 gccaaaattt tttaaaatat tttaatatta atttattgcc aaaatcataa tttattgtta
     4021 gtacagtaaa atgtaactaa ttactatgta tttttatatt taatttacaa ttcaaacaat
     4081 aaacttaaaa aaataaaaat gagaacaaat caactttcat tacttgtaat tggtggtatt
     4141 atattgttat taggtggctg gagctgttca aaatataatg gattggtaaa aacagatgta
     4201 gaagtgcaaa aagtttgggg taacgtagaa acgaactacc aaagaagaac cgacttatat
     4261 agtagtgtaa ttaaaactat tgaagggtct gctaatttcg aaaaaagcac tctaaaagat
     4321 gtaatcaatg cacgtgctgc tgctacacaa gttaaggtag atattaacga tcctgagtca
     4381 ttagcaaaat accaggcagc acaagggcaa ttacaaagtg catttggcag actaatggct
     4441 gtagcagagg cttatcctga tttaaaaact accaaatcgt ttcaagattt tcaggctcaa
     4501 atagaaggca cagaaaatag aattaatgta gcaagacaag attataacaa agctgttcaa
     4561 aactataatc taaaagtaag aactttccct aataatattt ttgcaggttt ctttggcttt
     4621 aaatctaaag agttttataa agcagatcct ggcacagaaa aagcacctga ggtaaatttc
     4681 aatatcaagt aatttttgtt atgttaccct tctttaaaaa gaaaccaact aattattttt
     4741 ctgaacctga aaagcaactt atagtaaatg ctataaaaca agcagaaaca aacactagtg
     4801 gcgagataag ggtttttatt gaaaacaaat gcagttatgt agatgctatt gatagagcaa
     4861 aagaattgtt tgagaaatta aaaatgtttg aaacagaaca acgcaatggc gttttgttat
     4921 atgttgccct aaaagataga cagttagcaa tttttggaga taagggaatt tatgaaaaaa
     4981 caggagttga gttttggaat aatgaagtaa atgaactttt actttttttt aataaattaa
     5041 actatgccga aggtatagct aatgtagtta ttcaaattgg caatgcacta aaactacatt
     5101 ttccttttaa taaatcttct gatgtgaatg aactgaaaga cgaaattgtt tttggaaagt
     5161 aattttttat acaaaaaata aatggctact gtaaactatt catgaaatat tttttctcca
     5221 tactattctc cttacttgtt aatatatctt ttgcacaaaa aagcattcct aaacctaatc
     5281 caccaaaatt agtaaatgat tatgcaggga tattagataa acacgatgcc gatttattag
     5341 aaaaaaaatt agttgcacta gatgatagta ccagcaatca aattgttgtt ttaactgtaa
     5401 aatcgctaaa cggagaaatt ttagaagatg tagcagtaaa cacatttaga gaatggggta
     5461 ttggcaataa aaaaacaaat aatggggttt taatactcat tgctttagaa gataaaaaag
     5521 ttagaattga agtaggctat ggtttagaag gtgcaatacc agatgttatt gctaacgaca
     5581 tattaaaaac atatcttgct cctgcattta agcaaggcaa ttatttagat ggtataaata
     5641 aagctgtaga taatattacc aaagcagctg ctggcgaata caaagtaaaa cgcaaaaaaa
     5701 gtaacgaaac aagtggaaaa agccttatta tttttataat aattttattt gttgtattaa
     5761 tgattgtagg caatagtgga agaggaggag gaggaggaaa tataatgaat agaggaggaa
     5821 gtaatgatat tgctacaaat ttatttttgt taagcctttt aaattcacgt tctggaagag
     5881 gaaacagtgg aggaggttgg ggaggaggag attggggtgg tggaggtttt ggtggttttg
     5941 gaggtggtag ttctggagga ggtggagcta gcggtggttg gtaaaaaatt aaacacaaga
     6001 tttatttttt taagcaagct attctaaaat ttagcttgct ttcttatttt tgcactcctt
     6061 taataaaaca ttttttagaa atattgtatg ggaagaattt ttgaggtaag aaaagcaacc
     6121 atgtttgccc gttgggacag aatggctaaa caatttactc gaattggtaa agaaatagca
     6181 atagcagtta aagctggtgg aacagatccg cacactaatc ctgcattgcg taggtgtatg
     6241 caaaatgcta aaagtgttaa tatgcctaaa gacagagtag aagctgcaat taaaaga
//
LOCUS       DAOQPK010000128         6286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_7228_length_6286_cov_4.047825, whole genome shotgun sequence.
ACCESSION   DAOQPK010000128 DAOQPK010000000
VERSION     DAOQPK010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7228_length_6286_cov_4.047825"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3151)
                     /locus_tag="PLU36_06945"
     CDS             complement(<1..3151)
                     /locus_tag="PLU36_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46523.1"
                     /translation="MRVSKFTLLSLLIFMGFVANAQLPIAYYDFEDNSARNTTIETTL
                     ETAVSTIGTPTVTDANGLTSSNGIGNGAGYGGSNTGYALGYYGFTTGSTVPTAAASDA
                     LPHIIFGPFTTTGIATLTLTMDLMGIGDKRPGNAVIYWSTDNVTYTRIATYPAIGTSF
                     STESFTLPAGAANQATLYIRVLGYNPSNTGTPDGTNGVMKIDNFTLRSSQINTTLTLA
                     NASTHGTGLTSGDTYLPIYSSFYLNSGTGNDVTMSSSLSLSGYVDLTSGNLVVGANTL
                     TFQVQTGNSTTGPIRKSGADAGFILFGTSSNMVIGDGTETATTTIPNNSFSAPSGMGN
                     LTINRNTATVNWGNNPIQITGDLTVTSGVFIKNVAAGTIDVDGDVVLSNIGDFRITSV
                     GLNVDGDMNISDDAFFRISATNANVTIAGSLSINDNGQVILSGNNTGSYVSVTVSTNL
                     NIGSAATLDLANNNAIIVVDGNVTGSGTISSAASNRRIVVTGATSTLSKDIFYGNLEI
                     NTGGTVSLLGNTFVTGFIRLTAGSLAVGAGNTLSLTAPTIVPIQRNGGSQLGLLTMSA
                     TSVLELGDGTQTATITLPNSFFTTPTTFATLTVNRTSGTLNWGNNPITLTSNANIDAG
                     TGYFIINNSLGIIGVGGNLNVNSGEFRIGVAANIPVTGNLNLVGDLGLTNAASNLVVT
                     GNVTGSGTHTAASLGQLELVGIGSTISNTITYDNVNINTGGTINLTGSTTIGGTLRLT
                     AGVLNVGAANTLTLTLNGSPVLRNGGSETGTITMAATSVFTCGDGSQTATTTLANSIF
                     TSPTSFATLNINRASGTTNWGNNPITLTGNAEITAGSGIFAINNASGELNVGGNLNIN
                     SGEFRVSVAANNTVTGNLHIVGTLGLTTPTAYVTVSGNVTGSGSQTAASTGRIIMVGT
                     GANTLASTVTYDNVEINTATGISLTGSTTFGGNLRLTLGNLNVGSNTITFHTDNTPLI
                     RTSGNLNLASGSSIRFGNAGNMGGNNFTIPDDVFTGSNIEFANFYMNRTNRITLNNQN
                     IAITGFLSVEAGMLALPAGY"
     gene            complement(3314..3472)
                     /locus_tag="PLU36_06950"
     CDS             complement(3314..3472)
                     /locus_tag="PLU36_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46524.1"
                     /translation="MRVSKFTLLSLLIFMGFVANAQLPIAYYDFEDNSARFLFLSSIL
                     LFYFIFFT"
     gene            3862..4857
                     /gene="pdhA"
                     /locus_tag="PLU36_06955"
     CDS             3862..4857
                     /gene="pdhA"
                     /locus_tag="PLU36_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HMZ46525.1"
                     /translation="MATKFSKETYLYWYELMQLIRQFELKAEEMYKMAGKIRGFFHAY
                     VGQEAIAAGCMTATRHEDPYITAYRDHGFALAKGTSANACMAELYGKATGCAKGKGGS
                     MHFFDVENYFFGGHGIVGAQIGTGAGLAFAEKYKGTDNVVLCFFGDGASRQGMLHETF
                     NMAMNWKLPVIFICENNHYAMGTSIERTSNVIDIYKLADAYEMPADQIDGMSPEAVHT
                     GIARAVKRAREGGGPTLLEMKTYRYKGHSISDPQKYRTKEEVEEYKEKDPIIQVANTI
                     LENNFANQEDLNNIDAKIAAIVEKSVQFAEESPWPDDSEVLKDVYADPNYPFIVD"
     gene            4900..5586
                     /locus_tag="PLU36_06960"
     CDS             4900..5586
                     /locus_tag="PLU36_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ46526.1"
                     /translation="MSEQIVKKDMWMQLESFWNKKKKIILTVGGAIVVIAGGLYGYKQ
                     YIVLPKEEKAADAIYKVQQFFQQDSSNLVINGNNETGAKGALYIIKNYSGTKAANLAY
                     YYAGISYLKLKDFNNAIKYLKDFSTNALQVQMSAYTALGHAYAESGKKDEAVSYYKKA
                     ATTFEEDKDNSAENLFLAAQLLEIMNKPKEALDIYKTIKQKYPQTSRGYEADKFIYRL
                     SIEKNDFSIN"
     gene            5589..6074
                     /gene="ribH"
                     /locus_tag="PLU36_06965"
     CDS             5589..6074
                     /gene="ribH"
                     /locus_tag="PLU36_06965"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HMZ46527.1"
                     /translation="MATKGNKNLNKGIPKLKDAFIVIIKTEWNSKIVNQLENGVIAVL
                     KENNINYKTIIVPGAIEIPFALKQHYTYSSTLPSAYITLGTVIKGDTPHFDYVCKFVT
                     EGILQLNLSLDVPVVFGVLTVNTEKEATERTTGSHGNKGEEFAITAIKMIALNNTLKK
                     L"
BASE COUNT         2250 a         1015 c         1029 g         1992 t
ORIGIN      
        1 catagcctgc tggtagagca agcataccag cttctacaga taagaatcct gttattgcaa
       61 tattttgatt atttaatgtt attctattgg ttctattcat atagaaatta gcaaattcaa
      121 tattgctacc agtaaacaca tcatcaggta ttgtaaaatt atttcctccc atatttcctg
      181 cattaccaaa ccttatacta gagccagagg ctaagtttaa atttccactt gttcttatta
      241 atggagtatt atctgtatga aaagttatgg tattggagcc aacatttaaa tttcctaaag
      301 ttaatcttaa atttcctcca aatgttgtag aacctgttag gctaatacct gttgcagtat
      361 ttatttcaac attgtcataa gttacagtac tagccaaagt atttgctcca gtaccaacca
      421 ttattattct gcctgtagaa gcggcggttt gagaacctga tcctgtaaca tttccactaa
      481 ctgtaacata agcagttgga gtagttaagc ctaaagtacc aacaatatgc aaattgcctg
      541 ttactgtatt atttgcagct acacttactc taaattctcc agaatttata tttagattac
      601 cgccaacatt taattctcca gatgcattat taatagcaaa aatacctgaa cctgctgtaa
      661 tctcagcatt gccagttaat gtaattgggt tattacccca gttggtagtg ccacttgctc
      721 tattaatatt taatgtagca aaacttgttg gtgaggtaaa aatggaattg gctaaggtgg
      781 ttgtagcagt ttgagaacca tcaccacaag taaatactga ggtggcagcc attgttatag
      841 taccagtttc cgaacctcca tttcttaaaa cagggctacc atttaaagtt aatgttaagg
      901 tgtttgctgc tcctacattt aaaacacctg cagttagacg caatgttcca ccaattgtgg
      961 tagaaccagt aagattaatt gttcctcctg tatttatatt aacattatcg tatgtaatgg
     1021 tatttgaaat ggtagaacca attccaacta attctaactg gccaagagag gcagctgtgt
     1081 gtgtaccaga gcctgttaca ttacctgtta caactaaatt ggaggcagca ttggttaatc
     1141 ctaaatcgcc tactaaattt aagttacctg taacaggtat attggcagct acgcctattc
     1201 taaactcacc actatttaca tttaaattcc ctcctacacc aataatacct aatgaattat
     1261 ttattataaa atatccagtt cctgcatcta tattagcatt gctagttaat gtaattgggt
     1321 tattacccca attcaatgta ccactcgtac gattaacagt taaagtagca aaggttgtag
     1381 gtgttgtaaa aaatgaatta ggaagtgtta tagttgctgt ttgtgtgcca tctcccaatt
     1441 ctaatactga tgttgcactc atggttagca acccaagttg agaacctcca tttctttgga
     1501 ttggaacaat tgttggtgca gttaaagata aagtattacc agccccaaca gctaagctac
     1561 ctgctgttaa tcttataaaa ccggtaacaa aagtattacc taataaacta acggtaccac
     1621 cagtattaat ttctaaatta ccataaaata tatcttttga taaagtagag gtagcaccag
     1681 ttacaactat tcttctatta gaggcagcac tagaaatggt accactacct gtaacattac
     1741 catcaacaac tattattgca ttattattgg ccaaatctaa agtagcagca gagccaatgt
     1801 ttaaattggt tgacactgta acactaacat aggatccagt attatttcct gataaaatta
     1861 cctgaccatt gtcatttata cttaaactgc cagctattgt aacattggcg ttagtagcag
     1921 aaatacggaa aaatgcatca tcactaatat tcatatcacc atctacgttt aggcctacgg
     1981 atgttattct aaagtctcct atgttgctta atacaacatc tccatctaca tctattgtac
     2041 cagcagccac atttttaata aacacaccag aagttaccgt taaatctcct gttatttgta
     2101 tagggttatt accccagttt actgtagcag tatttctgtt aatagttaaa tttcccatac
     2161 cacttggtgc agaaaaagaa ttatttggaa tggtagtggt tgctgtttct gttccatctc
     2221 caataaccat attggaactt gttccaaaaa gaataaaccc tgcatctgct ccagattttc
     2281 taattggtcc tgtagtacta tttccagttt gaacttgaaa agtaagcgta ttggcaccca
     2341 ctactaagtt tccagaagtt aaatcaacat atcctgaaag cgaaagggaa ctagacattg
     2401 ttacatcatt acctgtacca gaatttagat aaaaagaaga gtaaataggt aaataagtat
     2461 cgccagaagt taatcctgta ccatgagtac ttgcatttgc taaagtaagt gtagtattaa
     2521 tctggctaga tctaagcgta aaattatcaa ttttcattac accatttgta ccatcaggtg
     2581 tacctgtatt agatggatta taccccaata ctctaatata taaagttgct tggttggctg
     2641 cacctgcagg taaagtaaaa gattcggtag aaaaacttgt acctattgca gggtatgtag
     2701 caattcttgt ataggtaaca ttatctgtac tccaatatat tactgcattg cctggtcgtt
     2761 tatctcctat acccattaaa tccatagtaa gggtaagcgt tgcaatacct gttgtagtaa
     2821 atggtccaaa aataatatgt ggcaatgcat cagaagcagc agctgtaggc acagtagagc
     2881 cagttgtaaa accataatag cctaatgcat atcctgtgtt agagcctcca tatccagcac
     2941 cattgccaat accattagat gaagttaaac catttgcatc cgtaacagta ggtgtaccta
     3001 tagtagaaac ggcagtttct aaagttgttt caatagtagt atttctggca ctgttatctt
     3061 caaagtcgta atacgctata ggtagttgtg cgtttgcaac aaaacccata aaaattaata
     3121 aagataaaag tgtaaatttt gaaactctca tatatgtatt atttaaaaaa aatattttat
     3181 gaatattaga accgacaaaa atagcaaaat atagtacaaa gaaaacgttt tttgcgtaaa
     3241 atattgaatt attttcaaaa atattaaaca ccaaaagcac ttctatttta aagttttaag
     3301 ttaaaataaa attttatgta aaaaagataa aataaaacaa taaaatgctg cttaaaaata
     3361 aaaatctggc actgttatct tcaaagtcgt aatacgctat aggtagttgt gcgtttgcaa
     3421 caaaacccat aaaaattaat aaagataaaa gtgtaaattt tgaaactctc atatatgtat
     3481 tatttaaaaa aaatatttta tgaatattag aaccgacaaa aatagcaaaa tatagtacaa
     3541 agaaaacgtt ttttgcgtaa aatattgaat tattttcaaa aatattaaac accaaaagca
     3601 cttctatttt aaagttttaa gttaaaataa aattttatgt aaaaaagata aaataaaaca
     3661 ataaaatgct gcttaaaaat aaaaattggt taactctatc cgtgtttgac tataattagt
     3721 ctttacagtt taaccatgat acatttttac tttcaatatt attatctaac aagaaattag
     3781 attatttaga taaatcaata ctatattttt aattaagtaa tgcttaaacc ctatatttgc
     3841 caaccaaaat tttttgtact agtggcaact aaattttcaa aagaaactta tctatattgg
     3901 tacgaactca tgcaacttat tcgtcagttt gagttaaagg ctgaagaaat gtataaaatg
     3961 gctggaaaaa tacgtggttt ttttcatgcc tacgttggcc aagaagcaat tgctgcaggt
     4021 tgtatgacag ccaccagaca cgaagaccct tatattacag catatagaga tcatggcttt
     4081 gcacttgcaa aaggaacatc ggcaaatgca tgcatggccg aattgtatgg taaagcaact
     4141 ggttgtgcaa aagggaaagg cggtagtatg cacttttttg atgttgaaaa ttattttttt
     4201 ggtggacacg gaattgtagg tgcacaaatt ggaactggag ctggattagc atttgcagaa
     4261 aaatataaag gtactgataa tgtagtgctt tgtttttttg gggatggagc atctaggcaa
     4321 ggcatgcttc acgaaacgtt taatatggca atgaattgga agttgcctgt aatttttatt
     4381 tgcgaaaata accattatgc aatgggtact tctattgaac gtactagtaa tgtaattgat
     4441 atttataaat tagccgatgc ttatgaaatg cctgcagacc aaattgatgg catgagcccg
     4501 gaagctgtac atactggtat tgcaagagct gtaaaacgtg caagagaagg tggtggacca
     4561 actttgcttg agatgaaaac ttatcgctac aaaggtcatt caattagcga ccctcagaaa
     4621 tatagaacta aagaagaagt agaagaatac aaagagaaag atccaatcat tcaagtagct
     4681 aataccattt tagaaaacaa ctttgcaaat caagaagact taaacaatat tgatgcaaaa
     4741 attgctgcca tagttgaaaa aagcgtacaa tttgcagaag aaagcccttg gccagatgat
     4801 agtgaagtat taaaagatgt atatgctgac cccaactacc catttatagt agattaaggt
     4861 aaactaaaca atcaattaaa aaacaattat ttatatttta tgagtgaaca aattgtaaag
     4921 aaagatatgt ggatgcaatt agaaagtttt tggaataaaa agaaaaaaat tattcttact
     4981 gttggtggtg caattgttgt aattgctggc ggattatatg gttacaaaca atatattgtt
     5041 ttacctaaag aagaaaaggc tgctgatgct atttataaag tacaacaatt ctttcagcaa
     5101 gactcttcca accttgtaat aaatggtaat aacgaaactg gagcaaaagg tgctttatat
     5161 attattaaaa actatagtgg cactaaagca gctaatttag cttattacta tgcaggtatt
     5221 agttatttaa aactgaaaga ttttaacaat gctattaaat atttaaaaga ttttagcact
     5281 aatgctcttc aagtacaaat gagtgcatat acagctttag gccatgctta tgcagaatct
     5341 ggcaaaaaag atgaagcagt gagttattat aaaaaagctg ctacaacttt tgaagaagac
     5401 aaagacaact ctgccgaaaa tttattttta gcagcacagt tattagaaat aatgaataag
     5461 ccaaaagagg cattagatat ttataaaact atcaaacaaa aatatcctca aacaagccgt
     5521 ggatatgaag ctgataaatt tatttacaga ttaagtatag agaaaaacga ttttagcata
     5581 aactaattat ggcaaccaaa ggcaataaaa acttaaataa aggcatccca aaattaaagg
     5641 atgcctttat tgtaattatt aaaacagaat ggaatagtaa aattgtgaat caattagaaa
     5701 atggggttat tgctgtttta aaagaaaata atataaacta taaaacaatt atcgttcctg
     5761 gtgctattga aataccattt gcattaaaac aacattatac ttatagttct actttaccta
     5821 gtgcatatat tactttaggc actgtaatta aaggagatac tcctcatttt gactatgtat
     5881 gcaaatttgt aacagaagga attttacaat taaacctaag tttagatgtt cctgttgtat
     5941 ttggcgttct aacagtaaat acagaaaaag aagctaccga aagaactaca ggtagccatg
     6001 gaaataaagg agaggaattt gctataactg caattaaaat gattgcttta aacaatactt
     6061 tgaagaaatt ataatgattt taattccttc accacataaa aaagccttgt aatactacaa
     6121 ggcttttttg taaaccatct ataattaatt ccagcttata gatacatcat ctatagcggt
     6181 ggaaggtctg tttgcactac cagttgaatt tgaaagtgca gaaactctta tccaaacttt
     6241 ttgagattta ttgtcaatag catttccaaa atttacatta attgtg
//
LOCUS       DAOQPK010000129         6243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_7320_length_6243_cov_4.682127, whole genome shotgun sequence.
ACCESSION   DAOQPK010000129 DAOQPK010000000
VERSION     DAOQPK010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6243
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7320_length_6243_cov_4.682127"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(180..452)
                     /locus_tag="PLU36_06970"
     CDS             complement(180..452)
                     /locus_tag="PLU36_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46528.1"
                     /translation="MKYNQTTLNKLEDVLGESDYTVRYERGTFQSGWCVLELKKIVVL
                     NKFLNVEGRINTLLELIPQLDINYDKLTLETQKLYDQVMANNLALV"
     gene            complement(592..3021)
                     /locus_tag="PLU36_06975"
     CDS             complement(592..3021)
                     /locus_tag="PLU36_06975"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ46529.1"
                     /translation="MKKNVPFKALSFLFSLLVFSFASKAQLNPGSIAATSICTERNTD
                     APDIINNTLPSSGTAPYTYQWEAKIDYSGWAPVVSNGNGDSYSPGMLSYNTNFRRKVT
                     DANGNIAYSNEVRLYLTEGFTAGQIRLDNAVKDYNYYLPGSPIDLMIAAGARYGTDAY
                     SYQWETATALGGPWSAISGENGLTYQSPAINTVGSYFFRIKATDDNCGNIAYSDIIHI
                     QIVTTLPFFQGFFTHYYPCVFPNNTPSLLQGSLAIGGTGPYTYEWEKKGESDLTWQTI
                     ANSNTLTYQPGILTETTKFRRKATDANGNTGYTNESSIYIVTTVPNPGMIATNETAIA
                     PNAPYDAAINIQSASNFWNAGYAWQKSLDGGNTWTEINDYSYSTYFPPVTPTVVLTCY
                     RRSIREYCATNTRDTWTNTICIQPALPLTDGTISINGGNTACNTPGSQPLAITGTPAT
                     GGSTPYVYKWQTFDGTNWVDIANTNNVSYTPPVLNYSAKYRRIVTDANNTSLTSNEVL
                     VSIQGNSPLRGGLIDGPIVTCSNTAPGIINNIIDACGGGGVFTYSWEASTNNGPWTII
                     SGADQPTYNATNINETTKYRRKIGDGCGGATYSNEVEVFVYPPIEGGTITPAVQNVCT
                     NETPELLGLTQNCHYTNGNVTYQWQRATSPSGPWTNMNGATQAVLLPKASNTTAYYRL
                     VVKSSVCNAEAVSTVSVVNVNACSPKAANTATKLAATNLSTRGDMRLYPNPVTKGQTV
                     YVTFSHNSNTKNRVTATLRSIDGRTFNTIVEGSSNKGIQIKIPANIVQGTYLMQVTNG
                     EQKWIERVVIL"
     gene            complement(3146..3553)
                     /locus_tag="PLU36_06980"
     CDS             complement(3146..3553)
                     /locus_tag="PLU36_06980"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HMZ46530.1"
                     /translation="MMNNLPKTIIMFDGVCNYCNKMVNFCIKHDKKKKLHYTSLQSNT
                     GMSLISYYNIPKSIDSIILIDESKVYYYSDAALKIARHLNPPIRFIYIFKFIPTFLRD
                     ALYKWVAKNRYKWFGKKDTCMIPSKEVKHLFLE"
     gene            complement(3626..4783)
                     /locus_tag="PLU36_06985"
     CDS             complement(3626..4783)
                     /locus_tag="PLU36_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HMZ46531.1"
                     /translation="MSKLLVNAQENIDQPHAKMVSKFAFTQLTGGLVILHATIDTSSS
                     VLNFLLDTGSGGISLDSTTAKRLGFSIYPTERMIRGIAGKRKLSFTYGHYLNFPEFVT
                     DTFSFHINNYDLISSAYGYKIDGVIGYSFLRKYIVKVDYDMQMVEVYKPGIIKYPRGG
                     HILKPDFTTLPFTYFSLGNNKKIKSRYLFDMGAGLCFLLNKQFVKDSTLFNKRTKFYA
                     TQAEGLGGKKTLELAVIKNVSLGPYYFKDVPVYIFDDEFKVISYPKNTGIIGNDLLRR
                     FNLIINYPDKIIYIKPNKKFVDAFDYSYTGLGIYYIDDKIIVTDVIKGSPGDKAGFEA
                     GDIIVAIDNDYSNNIQTVKHALQNAGAKLNVLISRNEKLEMMILDVKNIMH"
     gene            4905..5516
                     /locus_tag="PLU36_06990"
     CDS             4905..5516
                     /locus_tag="PLU36_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HMZ46532.1"
                     /translation="MKVIILCNEIQEKEIISKPTATDVNVSFTNNKHTFYSTYADAYF
                     DLLADELNLEILRNDIPVFINSVIQTCKELPNNCIRLNAWNGFLKNEIIEIATLHHLE
                     YVEKVMNKLSWKYQVSSDDSGLIVPKIISMIVNEAYFALEDNISTKEEIDIAMKLGTN
                     YPYGPFEWSEKIGLKNIYFLLEKLSRTDNRYLPSKLLQTTALL"
     gene            complement(5535..>6243)
                     /locus_tag="PLU36_06995"
     CDS             complement(5535..>6243)
                     /locus_tag="PLU36_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HMZ46533.1"
                     /translation="ANFSTGRVYDQIRQSVAYSNKNVKICASHAGLTLGEDGATHQIL
                     EDIGLMKMLPGMTVIVPCDFNQTKAATKAIANYNGPVYLRFGRPKWANFTTEDGSDFI
                     IGKAQKLSDGNDVSIFACGHMVWKAIEAGKILEEKGISVEVINIHTIKPLDEDAIIQS
                     IQKTKCAVTVEEHNIIGGLGDSIAQVAAKYLPIPIEYIGTKDTFGESGKPTELLTKYG
                     LDTPFIVDATERVIKRK"
BASE COUNT         2064 a         1030 c          913 g         2236 t
ORIGIN      
        1 aaatggtaca ccatcggtag catatttatt gcaagataga agttacgaat atggcttacc
       61 aattggaatt aaaggcagac aaagaggtaa cagtaaacaa gctgatagtt atataactac
      121 tcaaatagga attagtataa atattggtag ttacaggtgc cctaagtatt aaaagttaac
      181 tacactaaag ccaaattatt tgccattacc tgatcgtaaa gtttttgagt ttctaaagtt
      241 aacttatcgt aattaatatc taattgtggt attaattcta aaagagtatt tattcttcct
      301 tcaacattta aaaatttatt tagtactaca atttttttca actctagtac acaccaacca
      361 ctctgaaaag tacctctctc gtaccttaca gtataatcgc tttcgcctaa aacatcttct
      421 aacttattta atgtggtttg attatatttc attaattatt aaaataaatt attcaatgtt
      481 tcaaaattac tgaaactgtt ttccattagc tgtttgtttt atccacacta ttgaataaaa
      541 aaaatccctg cattttttat atgcagggat taaaaattaa tctcaaaaat attacaaaat
      601 cactactctt tctatccatt tttgttctcc attggttact tgcattaagt atgtaccttg
      661 aacaatatta gcaggaattt ttatttgaat tcctttgttg cttgaacctt caacaatagt
      721 attaaatgtt ctaccatcaa tacttcttaa agtagctgta actctgtttt ttgtattgga
      781 gttatggcta aacgtaacat atacagtttg acctttagtt acagggttag gatataacct
      841 catatcacct ctggttgata agttagtagc tgctaattta gttgctgtat ttgcagcttt
      901 agggctacaa gcatttacat taacaacaga tactgtactt acagcttcag cattacaaac
      961 agatgattta actaccaatc tgtaatatgc tgttgtgtta gatgctttag gtaataaaac
     1021 agcttgtgtt gctccgttca tatttgtcca aggaccacta ggcgatgtag ctctttgcca
     1081 ttggtaagtt acattaccat tagtataatg gcagttttga gtcaatccta atagttcagg
     1141 agtttcattg gtacaaacat tttgaacagc aggtgtaatt gtaccacctt caattggtgg
     1201 gtaaacaaac acttctactt cattactgta agttgcacct ccacaaccat ctccaatttt
     1261 tcttctatat tttgttgttt cgttaatatt tgttgcattg taagttggtt ggtctgcacc
     1321 agaaataatt gtccatggtc cgttatttgt acttgcctcc caagaataag taaatacacc
     1381 accaccgcca caagcatcaa ttatattatt aattatgcca ggggcagtat tgctacaagt
     1441 tacaattggt ccatcaatta aaccacctct taatgggcta ttgccttgaa tagaaactaa
     1501 tacctcgtta cttgttaaag aagtattatt tgcatctgtt acaattcttc tatacttagc
     1561 actataattt aaaactggtg gagtataact aacattattt gtgtttgcaa tatccaccca
     1621 attggtacca tcaaaggttt gccatttata cacataagga gtactaccac cagtagcagg
     1681 tgtacctgtt atagctaatg gttgagaccc tggagtatta caagctgtat taccaccatt
     1741 aatgcttatt gttccatcgg ttaaaggtaa agctggttga atacaaatgg tatttgtcca
     1801 agtatctctt gtatttgttg cacaatattc tctaatggaa cgacgataac aagttaacac
     1861 aactgttggt gtaactggag ggaaataagt actataacta tagtcattaa tttctgtcca
     1921 tgtgttaccg ccatctaaac ttttttgcca agcataaccg gcattccaaa aattactagc
     1981 agattgaata ttgattgctg catcgtaagg ggcattaggt gcaatagcag tttcgttggt
     2041 tgcaatcatt cctgggtttg gtacagttgt aacaatataa atagaacttt catttgtgta
     2101 tcccgtatta ccgtttgcat cagttgcttt acgtctaaac ttagttgttt cagttaatat
     2161 tccaggctgg taggttaaag tattggaatt tgcaattgtt tgccaagtta aatctgattc
     2221 tccttttttc tcccattcat aagtataagg acctgttcct ccaattgcta ggctaccttg
     2281 taataaactt ggcgtattgt tagggaaaac acatgggtag taatgtgtaa agaagccttg
     2341 aaagaaagga agtgtagtaa ctatttgaat gtggattata tcggagtaag caatgtttcc
     2401 gcaattatca tctgttgctt ttattctaaa aaagtaactt cccactgtat taattgcagg
     2461 agattggtag gttaacccat tttctccaga aattgcagac catggaccac ctaaagcagt
     2521 tgcagtttcc cattggtaag agtatgcatc tgtgccatat cttgcacctg cagcaatcat
     2581 taagtctatt gggctacctg gtaaataata attataatct tttactgcat tatctaatct
     2641 tatttgacca gcagtaaaac cctctgttaa atataatctc acttcgttag agtatgcaat
     2701 gttgccatta gcatctgtta cttttcttct aaaattggta ttataagaaa gcattcctgg
     2761 actataagaa tcaccatttc cattacttac aactggagcc catccggagt agtctatttt
     2821 tgcctcccat tggtaagtat agggtgcagt tccagaactt ggtaaagtat tattaataat
     2881 atctggtgca tctgtatttc tttcagtaca aatagatgtg gctgcaatag agccagggtt
     2941 taattgagct ttggatgcaa aagagaacac taataaagaa aacaaaaagc ttaatgcttt
     3001 aaagggtaca ttttttttca taacgcttgt attaaataat taaacagcgt ttttcacgga
     3061 taaagtaaat gattcttatc taagaatata tggatgacta tataagagca aatttaatat
     3121 aaaatagttg ctttaaaatt cactttcatt ctaaaaacaa gtgttttacc tctttggagg
     3181 gtatcataca agtgtccttt ttaccaaacc atttatacct gtttttagct acccatttat
     3241 acaatgcatc tcttaaaaat gttggaataa atttaaatat atatataaac cttattggag
     3301 gatttaagtg tcttgcaatt tttaaagctg catctgaata gtaataaact ttactttcat
     3361 caataagtat aatagaatct attgattttg gtatattgta atagctaatt aaagacatac
     3421 cagtattgct ttgtagcgat gtataatgta attttttttt cttatcatgc tttatacaaa
     3481 agttaaccat tttgttgcaa tagttgcaaa cgccatcaaa cattataatt gttttgggta
     3541 aattattcat catcaaaaat tataaaataa aaaacaaaaa atgtccattt ctgtcttttt
     3601 ggggcatata aattatgcta ttatgttaat gcattatatt tttaacatct aaaatcatca
     3661 tttctaattt ctcattacga gaaattaaaa catttaattt agctcctgca ttttgcaatg
     3721 catgttttac ggtttgaata ttattagaat aatcattatc aattgctaca ataatatctc
     3781 ctgcttcaaa acctgcttta tcgcctggcg aacctttaat tacatcggtt acaattattt
     3841 tatcatcaat ataatatatt cctaaccctg tataagagta atcaaaagca tctacaaact
     3901 ttttgtttgg ttttatataa attattttat caggataatt aataattaaa ttaaatcttc
     3961 tcaataaatc gtttccaata attcctgtat tttttggata agatattact ttaaactcat
     4021 catcaaaaat atatacaggt acatctttaa aataataagg acctaaacta acatttttta
     4081 ttacagcaag ctctaatgtt tttttacctc ctaaaccttc ggcttgtgtt gcataaaatt
     4141 ttgttctttt attaaataga gtactatctt ttacaaactg tttattcaat aaaaaacata
     4201 atccagcacc catatcaaac aaatatctac tttttatttt tttgttatta cctaacgaaa
     4261 aataagtgaa aggtaatgtt gtaaaatctg gttttaaaat gtgcccacct ctaggatact
     4321 tgataatgcc aggtttatac acttctacca tttgcatatc ataatctacc ttaactatgt
     4381 atttgcgtaa aaaactatac ccaattacac catcaatttt atacccatat gcactagaaa
     4441 taagatcata attatttata tgaaaactaa aagtatcggt tacaaattca ggaaaattta
     4501 aataatgccc atacgtaaat gatagttttc tttttcctgc tataccccta atcattcttt
     4561 ctgttgggta aatagaaaag cctaaacgct ttgcagttgt agaatctaag gatattccac
     4621 cactgccagt gtctaacaaa aaatttaaaa cagaggagga agtatcaata gttgcatgca
     4681 aaataactaa gccaccagtt aattgtgtaa aagcaaactt tgaaaccatt ttagcatgtg
     4741 gctgatcaat attttcttgt gcatttacca ataatttact cattaacaaa aacaagagaa
     4801 aataatattt taaaatggag gatgtatagt tcatgaatta atttaaaatt attatccgtt
     4861 caacagactg cactttcttt acttttgctg cataatgtaa gaaaatgaaa gttataattc
     4921 tttgtaacga aatacaagaa aaagaaatta tttctaaacc tacagcaacg gatgttaatg
     4981 ttagttttac caacaataaa catacctttt atagcactta tgcagatgca tattttgatt
     5041 tattggcaga tgaattaaat cttgaaatat taagaaatga tattcctgtt tttataaata
     5101 gtgttataca aacttgcaaa gagttgccta ataattgtat aagattaaat gcatggaatg
     5161 gtttcttgaa aaatgaaatt attgagattg caacattaca tcacttagaa tatgtagaaa
     5221 aagtaatgaa taaattgagt tggaaatatc aagtatcgtc agacgactct ggtctaattg
     5281 ttcctaaaat aatttcaatg attgttaatg aagcttactt tgctttagaa gataatataa
     5341 gcactaaaga agaaattgat attgctatga agttaggaac caattatcct tatgggccgt
     5401 ttgaatggag tgagaaaatt ggtttaaaaa atatttattt tttattagaa aaattaagta
     5461 gaacagataa tagatatttg cctagcaagc ttttgcaaac tacagcatta ctttaatgaa
     5521 acaaatagca attcttattt tcttttaata actctttcag tagcatctac aataaatgga
     5581 gtatctaatc catattttgt aagtaactca gttggcttac cactttcgcc aaatgtatct
     5641 tttgtaccaa tatactcaat tggtattggt aaatattttg cagcaacttg tgcaatacta
     5701 tctcctaacc ctccaataat attatgctct tcaaccgtta ctgcacattt tgttttttgt
     5761 atagattgaa ttatggcatc ttcatctaat ggttttatag tatgtatatt aatcacttcc
     5821 acactaatgc ctttttcttc taaaattttt cctgcttcta ttgctttcca aaccatgtgg
     5881 ccacaagcaa aaatagaaac atcgtttccg tcacttaatt tttgtgcttt gcctataata
     5941 aaatcgctac catcttctgt agtaaaattt gcccattttg gtctgccgaa acgtaaataa
     6001 accggaccat tataattggc tatagcttta gttgcagctt ttgtttggtt aaaatcgcaa
     6061 ggtacaatta cagtcatacc tggtaacatt ttcattaaac caatatcttc taaaatttgg
     6121 tgtgttgctc cgtcttctcc taaagtaagc cctgcatggc ttgcacaaat ttttacattt
     6181 ttattactgt aagcaacact ttggcgaatt tgatcataca cacggccagt gctaaagtta
     6241 gca
//
LOCUS       DAOQPK010000130         6215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_7369_length_6215_cov_4.443831, whole genome shotgun sequence.
ACCESSION   DAOQPK010000130 DAOQPK010000000
VERSION     DAOQPK010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7369_length_6215_cov_4.443831"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="PLU36_07000"
     CDS             complement(<1..854)
                     /locus_tag="PLU36_07000"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HMZ46534.1"
                     /translation="MKTVKKIYSGKATNVGSIKVQELLPSIDGFLDPFLVLHHGIAIA
                     DENLPLKEQGVGPHPHRGFSPVSFIYKGGIHHRDSRNNNSVVYKGGAQWINAGMGIIH
                     SERLPEDILAHGGKQEIIQFWINTPAKFKMQQPAYFPVPSEEMPTIHSEDGLTTLRVP
                     AGKFENIKGTIPTFSDVTTIMGDIKKEGTYNFTFNTQHNSLLYVLEGELLVNEQTKIS
                     SKQLAVFNNDGEIFTIKANENTLFFVASGEPLNEPIAAHGPFVMNTQTEILEAFKDYQ
                     LGKMGVLI"
     gene            complement(904..1362)
                     /locus_tag="PLU36_07005"
     CDS             complement(904..1362)
                     /locus_tag="PLU36_07005"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HMZ46535.1"
                     /translation="MKLEKAIQSSKFKSEVQKAGLNILYTAWWLKTATSKELKLFGLT
                     HEQYNVLRILKGKHPEQMCVKDIAGRMIEKNSNVPRIIDRLVAKKLVKRSQCCNDGRQ
                     TVMELTTQGILILNNSNNKLDTLFENLMSLNNKEAQALNQLLEKLRENEL"
     gene            1487..2758
                     /gene="murF"
                     /locus_tag="PLU36_07010"
     CDS             1487..2758
                     /gene="murF"
                     /locus_tag="PLU36_07010"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HMZ46536.1"
                     /translation="MKIEELYHIYCNHSIICTDTRKIESNCIFFALKGENFNGNQFAL
                     EALNNGAAFVVVDEQVGISNDKIILVENVLYTLQQLAKYHRQQFTIPFIAITGSNGKT
                     TTKELVSAVLKTQYTTYTTQGNLNNHIGVPLTILSIKKDAEMAVIEMGANHQKEIESY
                     CLYTLPTHGIITNCGKAHLEGFGGEEGIKKGKGELFSFLKEHNGTAFVYNEYDYLLEM
                     AMGIKEIVEYGSSSTLFSASIVQFDPYLSIKFSTNVTTEICTNLVGKYNFSNVLTAVA
                     IGLYFNISMDKIKNAIVQYKPSNSRSQLLKIESNYFILDAYNANPSSMKVALENFAAI
                     KADNKVVMLGAMMELGIDSIKEHQQIADLLLQLKFKNVVLTGGDFKFTHHPFIYKENN
                     EAAKLWLQQNKFTNTYFLIKGSRSMQMEKIL"
     gene            2793..3224
                     /locus_tag="PLU36_07015"
     CDS             2793..3224
                     /locus_tag="PLU36_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HMZ46537.1"
                     /translation="MNQPSNNFLTAVLKNKCPRCRQGNLFLNKNPYKLKDGLLMHEKC
                     LVCGQPTEIEVGFYYGTGYLSYAISFAFLVATMVAWAVIFGLSFKDNSIFEWLITACI
                     LLVMLQPILMRLSRTLWLSFFVKYDANWKNNPVDKPERFIK"
     gene            3268..3355
                     /locus_tag="PLU36_07020"
     tRNA            3268..3355
                     /locus_tag="PLU36_07020"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3302..3304,aa:Ser,seq:gga)
     gene            3452..4183
                     /locus_tag="PLU36_07025"
     CDS             3452..4183
                     /locus_tag="PLU36_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HMZ46538.1"
                     /translation="MNKLTDSWFSPALNKEMPIVSYGFNGFAVLLVPTAAADYLEYER
                     FQMLEALAPLIDKGKLRVFSINSINNESWLNNEILPEHKAIRHNQFNDYVFNEVVPFI
                     RNMAGADTFIYTSGASFGALHSMNLFLKRPDIINGCISMSGVYDLTEYTKGFWDEQVY
                     FNSPFHYVPNLTDTWYIDKIKASHHIHIATGSGNYEDPEANKKFSQVLWNKGIWHDLD
                     VWGPDIHHDWPTWRAMLPYILEVKF"
     gene            4307..5557
                     /gene="pepT"
                     /locus_tag="PLU36_07030"
     CDS             4307..5557
                     /gene="pepT"
                     /locus_tag="PLU36_07030"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HMZ46539.1"
                     /translation="MIANYKHTVVERFLEYVQIDTQSNPNSTTHPTTEKQKNLSNLLA
                     QQLVLLGLQDVVADEYGYVYATIPSNTTKKVPVICFCSHVDTAPDCSGNNVKPIFHAN
                     YSGNDIILPDDDNQILSIQKYPYLKEHIGNSIITASGKTLLGADDKAGVAIIMDMVNY
                     LTTHSSIKHGTIKILFTPDEEVGQGTAKINLEKLGADFAYTLDGGELGTLEDETFSAD
                     GATITVHGVIAHPGYAKNKLVNALKIVGEILASLPENEFSPETTDGRQGFVHPTAIEG
                     IAEKASINFIVRDFETSKLKEHEQRLEAIAQKVVAKYKGATMQMDVFEQYRNMKEVLD
                     KYPQVVEHAAKAYKRAGLNVINEPIRGGTDGSRLSFMGLPCPNLFTGMQAIHSKNEWI
                     GVKDMEKAVEVLVHLAQIWEEEGV"
     gene            5583..>6215
                     /locus_tag="PLU36_07035"
     CDS             5583..>6215
                     /locus_tag="PLU36_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ46540.1"
                     /translation="MTTNTAVLSIQNLVKYYGSIKALDGISYDIPKGCVYGILGPNGS
                     GKTTTLGIVLNVLNQTSGTYLWFGNPANADTRKKIGSLLETPNFYHYLSAVDNLKITQ
                     SINKRGTEEDIHKVLEIVNLAQRKNSKFSTYSLGMKQRLAIAAALLGNPEVLVLDEPT
                     NGLDPVGIAEIRELIKSLAENGKTIIMASHLLDEVEKVCTHVAIMKKGVLI"
BASE COUNT         2156 a          897 c         1055 g         2107 t
ORIGIN      
        1 tcaattaata cgcccatttt gcctaattga taatctttaa aagcttctaa aatttctgtt
       61 tgggtattca ttacaaatgg tccatgtgca gcaattggct catttaatgg ttcgccactt
      121 gcaacaaaaa acaaagtatt ttcattagct tttattgtaa atatttcccc atcgttatta
      181 aaaacagcta attgttttga ggatattttt gtttgttcat tcactaataa ttcgccctct
      241 aatacataca gtaaactatt gtgttgggta ttaaaagtaa agttgtaagt tccttctttt
      301 tttatgtcgc ccattatagt agtaacatcc gaaaaagtag gaatagtacc ttttatattt
      361 tcaaattttc cagcaggtac tcttaaggtt gttaatccat cttctgaatg tatggtaggc
      421 atttcctcag agggaacagg aaaataggct ggttgttgca ttttaaattt tgcaggtgta
      481 ttaatccaaa attgaattat ctcttgttta cctccatgtg ctaatatatc ttctggaagc
      541 ctttcactgt gtattattcc catacctgca tttatccatt gtgcaccacc tttgtacaca
      601 acactattgt tatttctact atctctatga tgaatgcctc ctttgtaaat aaagcttaca
      661 ggactaaaac ctctatgggg gtgtgggcca acaccttgtt cttttaaagg caaattttca
      721 tctgcaatgg caataccatg atgtagtact aaaaagggat ccaaaaaccc atctatagat
      781 ggcaacaact cttgcacttt tatactgcca acattggttg cttttcctga ataaattttt
      841 ttaactgttt tcattttgtt tgttttttaa atatatgtat gtacatgtaa ttttataaaa
      901 aaattacaat tcgttttctc ttagtttttc aagtagttgg tttaatgctt gggcttcttt
      961 attatttaat gacattaaat tttcaaacaa agtatctaat ttattattcg aattatttaa
     1021 aattaaaatt ccttgtgtag ttaactccat tactgtttgt cttccatcat tacagcattg
     1081 gcttcgttta actaattttt ttgccaccaa tctgtcaata attctgggta cgttagagtt
     1141 tttttcaatc attctaccag caatatcttt aacacacatt tgttcaggat gcttaccttt
     1201 taatattctt aatacattat attgttcgtg agttaagcca aataatttta attctttgga
     1261 tgttgctgtt tttaaccacc aagctgtata taatatattc aaccctgctt tttgcacttc
     1321 acttttaaat ttactacttt gtattgcttt ttctaatttc atttctaaaa tgcaaatata
     1381 catgtatgta catgtatttc caaattttat tttttcaaac aaataaatca acaaaatctt
     1441 cagtgttaaa tctgcttatt tttgtgatat aaaatgtaat ataaatttga aaatagaaga
     1501 gttataccat atttattgca atcattcaat tatttgtacc gatacaagaa agattgaatc
     1561 aaattgtatt ttttttgctt taaagggaga aaattttaat ggtaaccaat ttgcattaga
     1621 agctttgaat aatggagctg cttttgtcgt ggtagatgag caagtaggta taagcaatga
     1681 taaaattatt ttggttgaaa atgttttata tactttacaa caattagcaa aataccatcg
     1741 gcaacagttt accattcctt ttattgcaat aacaggcagc aatggtaaaa caacaactaa
     1801 agaattggtt agtgctgttt taaaaactca gtacacaaca tatactacac aaggcaattt
     1861 aaacaatcat attggcgtgc ccttaactat tttaagtatt aaaaaagatg ctgaaatggc
     1921 tgtgatagaa atgggggcaa accatcaaaa agaaatagaa agctattgtt tatatacatt
     1981 gccaacacat ggcattataa caaattgtgg taaagcacat ttagagggtt ttggcggaga
     2041 agaaggaata aaaaaaggga aaggggaact gttttctttt ttaaaagaac ataatggtac
     2101 tgcatttgtt tataatgagt atgattattt attagagatg gctatgggaa taaaagaaat
     2161 agttgaatat ggttcttcat cgacattatt ctctgcaagc attgttcagt ttgaccctta
     2221 cttgtctatt aaatttagca caaatgttac tacagaaatt tgcactaatt tggttggaaa
     2281 atataatttc tcaaatgttt taacggctgt tgccattggt ttgtatttta atatatctat
     2341 ggataaaata aaaaatgcaa ttgtacaata caaaccatct aacagcagga gccagttatt
     2401 aaaaatagag agtaattatt ttattttaga tgcatacaat gccaatccaa gtagtatgaa
     2461 agtggcttta gaaaattttg ctgcaattaa ggcagataat aaagttgtaa tgcttggagc
     2521 tatgatggag ttaggaattg acagcataaa agaacatcaa caaattgcag atttattatt
     2581 gcagttaaaa tttaaaaatg ttgtattaac aggcggagat tttaaattca cccatcatcc
     2641 atttatttat aaagagaata atgaagcagc taagttgtgg ctgcagcaaa ataaatttac
     2701 caacacttat ttcttaatta aagggtcgag atctatgcag atggaaaaaa ttttataact
     2761 attttgcccg aataaaaatt taactttgtg gtatgaacca gccctctaat aatttcttaa
     2821 ctgctgtact taagaataaa tgcccaagat gcagacaagg aaacttgttt ttaaacaaaa
     2881 acccatataa acttaaagat ggtcttttaa tgcatgaaaa atgtttggtt tgtggccaac
     2941 caactgaaat tgaagttggt ttctattatg gcacaggcta tttaagttat gcaatttctt
     3001 ttgcgttttt agtggcaaca atggtagcat gggctgtgat atttggattg tcttttaaag
     3061 ataatagtat ttttgaatgg cttattactg cttgcatact gctagtaatg ctgcaaccaa
     3121 ttctaatgcg attatctaga acactttggc tcagtttttt tgtgaaatat gatgctaatt
     3181 ggaaaaataa ccctgtagat aagccagaaa gatttataaa ataactttca ttaagagtag
     3241 aaactttaca tttgcctact ttttaaaaga ggggtgtccg agtggttgaa ggagcacgcc
     3301 tggaaagtgt gtattcgtct aaagcgaatc gagggttcga atcccttccc ctctgcttcc
     3361 ttattccaat ttattaattc ctatttattt caattttttt tgataacatt gttataaata
     3421 tatcttattc tttaattgaa aattaactgt tatgaataaa cttacagatt catggtttag
     3481 cccagcctta aataaggaaa tgcctattgt gtcgtatgga ttcaatggtt ttgctgtatt
     3541 attagtacct actgcagctg cagattattt agaatacgaa aggtttcaaa tgttagaagc
     3601 attggcacca ttaatagata aaggaaaact tagagttttt agcattaaca gtattaataa
     3661 tgaaagttgg ttaaataatg aaatattgcc agagcataaa gcaatacgcc ataatcagtt
     3721 taatgattat gtttttaacg aagtagtacc ttttattaga aatatggcag gtgcagatac
     3781 ttttatttat accagtggtg caagttttgg agcattgcac agtatgaatt tatttttaaa
     3841 aaggccagat attattaatg gttgtattag catgagtgga gtgtatgatt taacagagta
     3901 caccaaaggc ttttgggacg aacaagttta ttttaacagc ccttttcatt atgtgccaaa
     3961 cttaacagat acttggtata ttgataaaat taaagcaagc catcatatac atattgcaac
     4021 aggtagtggt aattacgaag atcctgaagc caataaaaaa ttcagccaag ttttgtggaa
     4081 taaaggaatt tggcatgatt tagatgtatg gggcccagat attcatcatg actggcctac
     4141 ttggagagca atgttgcctt atattttaga ggttaagttt taaaattttt tataaatatc
     4201 ttatagagta aagtaatcaa ccactttact ctttttatta aaaattacac caaaggcatt
     4261 tatttaagaa tgctttattt tatcttgccc taaattatta ttattaatga tagcaaatta
     4321 taaacataca gttgttgaaa ggtttttgga atatgtgcaa atagatacac aaagtaatcc
     4381 aaatagtact acccatccta caacagaaaa gcaaaaaaac ttatccaact tattagccca
     4441 acaattagta ttattgggtt tacaagatgt agttgcagat gaatatggct atgtatatgc
     4501 aaccatacca tctaacacaa ctaaaaaagt acctgtaata tgtttttgca gccacgtaga
     4561 cactgcacca gattgtagcg gaaataatgt aaaacctatt tttcacgcta actactcagg
     4621 aaatgatata attctaccag atgatgataa ccagatttta tctatccaaa aatatccata
     4681 tttaaaagag catattggca actcaataat tacagcaagt ggtaaaacat tgttaggtgc
     4741 cgatgataaa gctggtgtag caattattat ggatatggtg aattacttaa ctacacattc
     4801 atcaataaaa catggtacaa taaaaatatt atttacacca gatgaagaag taggacaagg
     4861 aactgcaaaa attaatttag aaaaacttgg ggcagatttt gcttatactt tggatggagg
     4921 agaattgggt actttagaag atgaaacctt tagtgcagat ggagcaacca ttactgttca
     4981 tggtgtaatt gcacatcctg gttatgcaaa aaataaatta gtaaatgctc taaaaattgt
     5041 aggagaaatt ttagctagtt tacccgagaa tgaatttagt ccagaaacaa cagatggcag
     5101 acaaggattt gtacacccaa cagccattga aggtattgca gaaaaagcaa gcattaattt
     5161 tattgttaga gattttgaaa caagcaaact aaaagagcat gaacagagat tagaagcaat
     5221 tgcacaaaaa gttgttgcta aatataaagg ggcaaccatg cagatggatg tttttgaaca
     5281 atatagaaat atgaaagaag tattagataa atatccacaa gtagttgagc atgcagccaa
     5341 agcttataaa cgtgcaggat taaatgttat taatgagcca atacgaggag gaacagatgg
     5401 cagccgttta agttttatgg gcttaccctg cccaaattta tttacaggta tgcaggcaat
     5461 acatagcaaa aatgaatgga ttggtgtaaa agatatggaa aaagctgtag aagttttggt
     5521 acaccttgct caaatttggg aagaagaagg agtgtgattt tataagacaa ttaattaata
     5581 atatgactac aaatacagct gttttatcca tacaaaattt agtaaaatat tatggctcca
     5641 taaaagccct agatggtata tcttacgata ttccaaaagg ctgtgtttat ggtattttgg
     5701 gccccaatgg tagtggcaaa acaacaacct taggtattgt tcttaatgta ctaaaccaaa
     5761 ctagtggtac ttatttatgg tttggaaacc ctgctaatgc cgataccaga aaaaaaattg
     5821 ggagcttatt agagacacct aatttttatc attacttgtc tgcggttgat aatttaaaaa
     5881 ttactcaatc cataaacaaa agaggaacag aagaagatat acataaagta ttagaaatag
     5941 taaatttagc tcaaaggaaa aacagcaaat tcagtaccta tagtttgggt atgaaacaaa
     6001 ggttggctat tgctgctgct ttacttggta atccagaggt tttagtttta gatgaaccaa
     6061 ctaatggttt agatcctgta ggcattgctg aaattagaga attaattaaa agtcttgcag
     6121 aaaatggtaa aactattatt atggcaagcc acttgttaga cgaagttgaa aaggtatgca
     6181 cccatgtagc tatcatgaaa aaaggtgttt taata
//
LOCUS       DAOQPK010000131         6170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_7458_length_6170_cov_3.639738, whole genome shotgun sequence.
ACCESSION   DAOQPK010000131 DAOQPK010000000
VERSION     DAOQPK010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7458_length_6170_cov_3.639738"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PLU36_07040"
     CDS             complement(<1..608)
                     /locus_tag="PLU36_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HMZ46541.1"
                     /translation="MLVNTETYKKITEELINKATLVAVSKVKPVEAILELYHLGQRDF
                     GENYVQELVDKQSKLPTNINWHFIGHLQSNKVKFIAPFVYLIHGVDSIKLLKEVNKQA
                     AKNNKIIKCLLQVHIAQETTKFGFGLDELNDLFFLSECKNVEVIGLMGMASFSNDLNL
                     VRKEFIQLQQLFKTLQKSNPQFTTLSMGMSDDYKIALEEGSN"
     gene            complement(611..1888)
                     /gene="serS"
                     /locus_tag="PLU36_07045"
     CDS             complement(611..1888)
                     /gene="serS"
                     /locus_tag="PLU36_07045"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HMZ46542.1"
                     /translation="MLQVNYIRQEKEKVKERLQVRNFSNVEIVDSIIALDDTRKNIQL
                     NFDNTQAKINATSKEIGKLMAQGNKDEAEQIKQNVAQWKTEVTNLNTNLINIEKKLQD
                     ALIQLPNLPHESVPKGKTAEDNVTVKEAGKKPVLPANALPHWDLIKQYNLVDFETGAK
                     ITGSGFPLYKGKGAKLQRALVQYFLDYNTSAGYTEFLPPFMVNETSAYSTGQLPDKEG
                     QMYHATEDNFYLIPTAEVPVTNIYRDVILKESDLPIKMTAYSPCFRREAGSFGKEVRG
                     LNRVHQFEKVEIIQIVEPEKSYSVLDEMVAHVEALLTNLELPYRILRLCGGDMGFTSA
                     LTYDFEVWSAAQQRWLEVSSVSNFENFQTNRMKCRYKNAVGKTVLAHSLNGSSLALPR
                     IYASLLENNQTDAGIRLPKALHNYFGDEIITKN"
     gene            2072..3022
                     /locus_tag="PLU36_07050"
     CDS             2072..3022
                     /locus_tag="PLU36_07050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ46543.1"
                     /translation="MQKSLINFLENTPTFYFVRFDLNGKLTYVNQYYQKHFEQFFIHF
                     LGANFTDSIHPDSIEDFNKSRQQCIDKANCIIKVELRDNKIININTPNYIKWNFFSII
                     NESGEVVEFGGFGVSTSSVRKAKEEHKVTQQKLVALLNSTVESFYYLDKNMNVLAFNS
                     GAKYKTMLYYNIELQEGFYFPKLLVPGTEENFYKQFSNALKGVENYIEDKISYSNGKS
                     IWYKMSCKPVYDDKNNIMGVALSFLNIDDIKNAELRLKKIAWQQSHNVRKPLSNILGL
                     VNLLKEDKDIAPNIKDLIDLLDVSAKELDDIIKSIIAKTI"
     gene            3037..3978
                     /locus_tag="PLU36_07055"
     CDS             3037..3978
                     /locus_tag="PLU36_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46544.1"
                     /translation="MHHSLINFFENTPSIYIISFDLQDKFTYCNTMFKKQFTSSATNK
                     NIENFLDVIHEESVNLYQETKQKCITDASTIVKVELKKKLANNIHNYIQWNFFAIANA
                     DNVVNEIGAIGYDITENNNEKEGHKTTIQKLHAVLNSSDESFYFLDKEMKVLSYNLGA
                     KKASSLLYNINLQEGYDFRNSLLPGTEENFCTMFKNALNGMESEMENEQIFPTGKNIW
                     YKLIMKPVLNDSNIVIGVAVNFINIDKIKKSEQILNEIAWQQSHYVRKPVSNILGLVN
                     LLKNEKDSKKSKELLDMLDASTKELDEVIKNIIVKTL"
     gene            4053..4397
                     /locus_tag="PLU36_07060"
     CDS             4053..4397
                     /locus_tag="PLU36_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46545.1"
                     /translation="MYIQDDLQQRWWALEAKLVERFGKRPDMESILFLIGLQETGLSS
                     KKISKEQKQDLMHVAVCTLLQPSGYFAFEKKDKDNWPHFKQLKEIPNYNLPDQENFLK
                     DHVLLYFEQIGF"
     gene            4417..5097
                     /locus_tag="PLU36_07065"
     CDS             4417..5097
                     /locus_tag="PLU36_07065"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMZ46546.1"
                     /translation="MKKLALLILTFISLQVVAQKAINSKPKERLVEIKTDYGTMIVRL
                     YNSTPLHRDNFIKLVEQGFYDSLLFHRVIKDFMIQGGDPTSKLADSLAVLGGGSAPGD
                     RIPAEFRANLIHKKGVLAAARDGNPEKASSNCQFYLVQGKKIDSTQLNQTYETRIKPN
                     APNFSYTKQQKEIYKRIGGTPFLDQNYTVFGEMISGLNVLDKIAHVPTKPGDRPIKDV
                     RMKIKMLN"
     gene            5161..5538
                     /gene="gcvH"
                     /locus_tag="PLU36_07070"
     CDS             5161..5538
                     /gene="gcvH"
                     /locus_tag="PLU36_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HMZ46547.1"
                     /translation="MNFPSNLRYTKEHEWVSIDGNTATIGITEFAQSELGDIVYVDIN
                     TVGNSLQANEVFGTIEAVKTVSDLFLPVAGTVSEVNKALEKNPELVNNDPYGEGWIIK
                     LTVNNISDIDTLLDSAGYAAVVG"
     gene            5637..6128
                     /locus_tag="PLU36_07075"
     CDS             5637..6128
                     /locus_tag="PLU36_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HMZ46548.1"
                     /translation="MPSFDIASKVDIQTLDNAINVVKKEITNRFDFRDSHVVIELNKK
                     DYTVLLEADSDMKLQQIIDVLISRAMKQGIDGNAFDFSKDAYPSGKVVKKNVSVKNGL
                     KQEDAKKIVKAIKDSGLKVQPAIMDNIIRVTAKKIDDLQAVIALCNNGNFGVPLQYEN
                     MKS"
BASE COUNT         2252 a          865 c          920 g         2133 t
ORIGIN      
        1 atattgctac cctcttccaa agcaatttta taatcatcgc tcatacccat tgataaggta
       61 gtaaactgtg ggttactttt ttgtaatgtt ttaaaaagtt gttgtagttg tataaattct
      121 tttcttacta aattcaaatc attgctaaaa gatgccatac ccatcaagcc aattacttca
      181 acatttttac attcgcttaa aaaaaataaa tcatttaatt catctaatcc aaatccaaat
      241 tttgtggttt cttgtgctat gtgtacctgt aataaacact tgataatttt attatttttt
      301 gcagcttgtt tattaacttc ttttaatagt ttaatactat ctacaccatg tattaagtaa
      361 acaaaaggag caataaattt tactttatta ctttgcaaat gccctataaa atgccaatta
      421 atattggtag gcaatttact ttgtttgtca actaattctt gtacataatt ttcgccaaaa
      481 tctctttgcc ccaaatggta taattctaat atggcttcca caggctttac cttacttaca
      541 gctacaagtg ttgctttgtt aattaattcc tctgtaattt ttttatatgt ttctgtatta
      601 acaagcatct ttagttttta gtaattattt catctccaaa ataattatgc aatgctttag
      661 gtaatcttat tccagcatct gtttgattgt tttctaatag agatgcatat attcttggca
      721 atgccaaaga actaccattt aaactatgag caagtacagt tttgccaact gcatttttgt
      781 acctgcattt catacggtta gtttgaaaat tttcaaaatt gcttacacta cttacctcaa
      841 gccaacgttg ttgtgctgca ctccacactt caaagtcgta tgtaagagca gatgtaaagc
      901 ccatatcgcc accacataaa cgtaaaattc tgtatggcaa ttccaggtta gttaacaagg
      961 cttctacatg tgcaaccatt tcatctaaaa cagaataact tttttctggt tcaacaatct
     1021 gaataatttc tactttttca aattggtgca ccctattcaa gcccctcact tcttttccaa
     1081 aactaccagc ctctcttcta aaacaaggtg agtaggcagt cattttaatg ggtaaatcac
     1141 tttcttttaa aataacatct ctgtaaatat tggttacagg cacttcggca gtgggtatta
     1201 aataaaaatt atcttcagtt gcatgataca tttgcccttc cttatctggt aattgcccag
     1261 tactgtatgc actagtttcg ttaaccataa atgggggtaa aaattcagtg taacctgcac
     1321 tagtattata gtctaaaaag tactgcacta aagctctttg caactttgca cctttgcctt
     1381 tgtataaagg aaaaccactt cctgtaattt ttgctcctgt ttcaaaatca actaaattat
     1441 attgttttat taaatcccaa tgtggcaaag catttgcagg caatacaggt tttttaccag
     1501 cttcttttac tgttacatta tcctctgctg tttttccttt aggtacagat tcatgaggta
     1561 aattgggtaa ttgtattaaa gcatcttgca gttttttttc aatattaata aggttagtgt
     1621 ttaagtttgt tacctctgtt ttccattgtg ctacattttg tttaatttgt tctgcttcat
     1681 ctttgtttcc ttgtgccatt aatttaccaa tttctttact ggtagcatta atttttgctt
     1741 gggtattatc aaaatttaac tgaatgtttt ttcttgtatc atctaaagca ataatactat
     1801 ctacaatttc tacgttcgaa aaatttctta cctgcaatct ctcttttact ttttctttct
     1861 cctgccttat ataatttacc tgaagcataa aatttaattt gccgcaaacg tacaaaattt
     1921 tattttactt gaaataattc agctattgct gatgttttta gttgtttcat ataaatagtt
     1981 aaccatttaa aaagtaaaat ttgtttttta aaaatagaat attaagtttg agaaccttta
     2041 aaacctaata accaaagaaa gatttattga aatgcaaaaa tccttaataa attttttaga
     2101 gaatactcca acattctatt ttgtaagatt tgatttaaat ggaaaactaa cttatgtaaa
     2161 tcaatactat caaaaacatt ttgaacaatt ttttatacat ttccttggtg caaattttac
     2221 tgactccatt cacccagata gtattgaaga ttttaataaa agtaggcaac aatgtataga
     2281 taaagctaac tgtataatta aagtagagct tagggataat aaaataataa atataaacac
     2341 ccctaattat ataaaatgga attttttttc tattattaat gaatctggtg aagtagttga
     2401 atttggtgga tttggggttt ctacatctag tgttagaaaa gctaaagaag agcataaagt
     2461 tacacaacaa aaattagttg cactattaaa cagcacagta gaatcttttt actatttaga
     2521 taagaacatg aatgtacttg cttttaatag tggtgcaaag tataaaacca tgttgtatta
     2581 taatattgaa ttacaagaag gtttttattt tcctaaacta ttagtacctg gtaccgaaga
     2641 aaatttttac aaacaatttt caaatgcatt aaaaggtgtt gaaaattata ttgaggataa
     2701 gatttcttat tctaatggaa aatctatttg gtataaaatg agctgtaaac ctgtgtacga
     2761 cgataagaat aatattatgg gggttgctct aagtttttta aatattgatg atattaaaaa
     2821 tgccgaactt aggttgaaaa aaatagcatg gcaacaaagt cataatgttc gtaagccttt
     2881 atccaatatt ttggggttag taaatttatt gaaagaggat aaagacattg cccctaacat
     2941 aaaagattta atagatttat tagatgtaag tgctaaagaa ttagatgata ttatcaaatc
     3001 tattattgct aaaacaattt aattttctaa aaataaatgc atcattcttt aattaatttt
     3061 tttgaaaaca caccaagtat ttatattata agttttgatt tacaagataa gtttacttat
     3121 tgtaatacaa tgtttaaaaa gcagtttact agcagtgcaa caaataaaaa tatcgaaaat
     3181 tttttagatg taattcatga agaaagtgta aatctttacc aggaaactaa gcaaaaatgt
     3241 ataacagatg ctagtacaat agtaaaagtg gagctaaaga aaaaattagc taataatatt
     3301 cataactata tacaatggaa tttttttgca attgcaaatg cagataatgt tgtgaatgaa
     3361 ataggtgcaa ttggttatga tataaccgaa aataataacg aaaaagaagg gcataaaaca
     3421 actattcaaa agcttcatgc ggtattaaat agctccgacg aatcatttta ttttttagat
     3481 aaagaaatga aagttctttc ttacaattta ggagctaaaa aagcatcctc tcttttatac
     3541 aatattaatt tacaagaagg gtatgatttt agaaattcat tattgccagg tacagaagaa
     3601 aatttttgta caatgtttaa gaacgcctta aatggaatgg aatctgaaat ggaaaatgaa
     3661 caaatttttc ctacaggtaa aaatatttgg tataagctta taatgaaacc tgttttaaac
     3721 gatagcaaca ttgtaattgg cgtagcagta aattttatta atatagataa aattaaaaaa
     3781 agtgaacaaa tactaaatga aattgcatgg caacaaagcc attatgttcg caaacctgtt
     3841 tcaaatattt tgggtttagt aaatctatta aaaaatgaaa aagatagtaa aaagtctaaa
     3901 gagttgttag atatgttaga tgcaagtaca aaagaattgg atgaagttat taaaaacatt
     3961 attgttaaaa cattataatg ttaacctatc attcataaag cctaagcgta attttaatta
     4021 ttttttttaa gcttatttaa tacatttgcc caatgtatat acaagatgat ttacaacagc
     4081 gttggtgggc attagaagcc aaactggtag aaagatttgg caaaagacca gatatggaat
     4141 ctatattatt tttaataggg ttgcaagaaa caggccttag ttcaaaaaaa atttctaaag
     4201 agcaaaagca agatttaatg catgtggcag tttgcacttt acttcagcca agtggttatt
     4261 ttgcttttga aaaaaaagat aaagataatt ggccccattt taaacaactt aaagaaatcc
     4321 ctaattacaa tttacccgat caagaaaatt ttttaaagga tcatgtatta ctttattttg
     4381 aacaaatagg tttttaaaaa actgcacctt aataaaatga aaaaattagc actattaata
     4441 ttaaccttca ttagcctaca agttgtagct caaaaagcaa taaactcaaa gccaaaggaa
     4501 cgtttggtag aaattaaaac cgattatgga actatgattg ttcgtttata caattctact
     4561 ccattacatc gagataattt tataaaatta gtagaacaag gattttacga tagcttatta
     4621 tttcatagag taattaaaga ttttatgata caaggtggag atccaacaag caaactggca
     4681 gatagtttag ctgtattggg tggaggatct gcccctggag atagaattcc tgctgaattt
     4741 agagctaacc taattcataa aaaaggagtt ttagctgcag caagagatgg taatccagaa
     4801 aaagcaagta gcaactgtca gttttattta gttcaaggaa aaaaaataga ttcaactcaa
     4861 cttaatcaaa cttacgaaac ccgtataaaa ccaaatgctc ctaattttag ttacaccaaa
     4921 caacaaaaag aaatttataa acgaattggg ggcacaccct ttttagatca gaattatacc
     4981 gtttttggcg aaatgatttc tggcttaaat gtattggata aaattgcaca tgttccaaca
     5041 aaacctggcg acaggcctat aaaagatgta agaatgaaaa taaaaatgtt gaattaattt
     5101 aatttcattt gaattaatag taaaaaaact aatctttgca caaataaaac tttcacaatt
     5161 atgaactttc cttcaaacct acgttataca aaagaacatg aatgggttag tattgatggc
     5221 aatactgcta ccattggcat tactgaattt gcacaaagtg aattgggtga tattgtttat
     5281 gtagatataa atacagttgg caatagttta caagccaacg aagtgtttgg tacaattgaa
     5341 gccgtaaaaa ctgttagcga tttattttta cccgttgcag gtactgtttc tgaagtgaat
     5401 aaagctttag aaaaaaatcc ggaattggtt aataacgacc cttatggaga aggctggata
     5461 ataaaactta ctgtaaataa tatttcagat atagatactt tattagatag tgcaggatat
     5521 gctgctgtgg taggataatt taataattac ttttttatac cacataaatt acaggcttat
     5581 agtactttta aacttgtagt ttatgtggtt tattttttaa tactaaaatt tattgtatgc
     5641 catcttttga cattgccagc aaagtagata ttcaaacctt agacaatgct attaatgtag
     5701 taaaaaaaga aataactaat aggtttgatt ttagagatag ccatgtagtt atagaattaa
     5761 ataaaaaaga ctatacagtt ttattagagg ctgacagcga tatgaaatta cagcaaataa
     5821 tagatgtact tataagcaga gcaatgaagc aaggtataga tggcaatgct tttgatttta
     5881 gtaaagatgc ttacccaagt ggtaaagtag taaagaaaaa tgtaagtgta aaaaatggtt
     5941 taaaacaaga ggatgcaaaa aaaatagtta aagcaattaa agacagtggc ttaaaagttc
     6001 aacctgctat tatggataat ataataagag ttactgctaa aaaaatagat gatttacaag
     6061 ctgtaattgc tctatgtaat aatggaaatt ttggtgtgcc tttacaatac gaaaatatga
     6121 aaagctaata atccaaattc attatcgttc aaaatatcat caatcgaagt
//
LOCUS       DAOQPK010000132         6104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_7583_length_6104_cov_5.044305, whole genome shotgun sequence.
ACCESSION   DAOQPK010000132 DAOQPK010000000
VERSION     DAOQPK010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7583_length_6104_cov_5.044305"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            130..1074
                     /locus_tag="PLU36_07080"
     CDS             130..1074
                     /locus_tag="PLU36_07080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ46549.1"
                     /translation="MEDKLKMFENRLLKVYKHKSKQAKRQEVCCYRVYDHDLPEFPFC
                     IELYEDKIYLAEYVRRHGMTDEEHEEWLEQCIVLICKLLNVPDENVFVKQRKRKSNRL
                     DQYEKTGEEKTFFTVLENGLKFLVNLSDYLDTGLFLDHRITRSMVKEQCAGKRVLNLF
                     AYTGSFSVYAAAGNAASVTTVDLSKTYLQWAEDNFTINHFKDKNKYNFIHADVKQYLK
                     TLQPNSFDLVIIDPPTFSNSKRMKDFLDTQRDHAELLNDILFALSENGVIYFSTNYTK
                     FVMDTENIKASYIKDITKATTPFDFEGKLKRWCFKIGK"
     gene            1134..1856
                     /locus_tag="PLU36_07085"
     CDS             1134..1856
                     /locus_tag="PLU36_07085"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HMZ46550.1"
                     /translation="MLKYFFAFCIISICFSSCFIFGYRKIKGNGIYTTEKRNLSANKI
                     KVEGNLDIQIAQSNEPGVNIKADANLLEYIITELEDESLVIKTKRKYKLVSENPIIVE
                     VNTALLKSISIAGKSNVKGLTKFEGGEKLDIKIAGKGNVQLAVNTPKVEVKLAGVGEI
                     NLEGETKEVIVKISGSGNFKGKNLKAENATIKIAGSGDASVFASENLDVKISGSGNVE
                     YIGSPNIDQKISGSGTIKPIKE"
     gene            1984..3240
                     /locus_tag="PLU36_07090"
     CDS             1984..3240
                     /locus_tag="PLU36_07090"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HMZ46551.1"
                     /translation="MKVFKFGGASANSIERIQNIPEILQQYSTEQIVMVISAMGKTTN
                     ALEKVAEAFYNGNNIQALDLFEIVKQHHINTAKYLMITNYNNCINQLNDYFTEVEWLL
                     HDKPTQQYDYYYDQIVCIGEMMSTCVISCYFTEIGIGNTFLDVRDILKTDDNFRDAAV
                     NWEKTTANLKEIVLHPSLTIIQGFIGSTDENQSTTLGREGSDYTAAIFANLLHADSLT
                     IWKDVEGVMNADPKEFEDAQLINELSFNEVIEMAYYGAQVIHPKTIKPLQNKNIPLYV
                     KSFLDSSLKGTIISKQNNKNLPPIIVIKNNQVLMHLHTQDFSFVGEEPMAALYKIFST
                     LKIQPNLIQTGAISVQICLDNKEDKIEQLAINAASLFEVKVEKELQLLTIRHFTEELK
                     NKMLHNKNVILLQQTKQTLQALYTIE"
     gene            3298..3579
                     /locus_tag="PLU36_07095"
     CDS             3298..3579
                     /locus_tag="PLU36_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HMZ46552.1"
                     /translation="MAQYSLGDAMKKFIKQSNLRNGLRAVQIEEVWEEVMGKTIAKYT
                     DKIEIVNQTLFIKTQIGSLKNELMYQKPLIIERINEKFGEKIIATVVIQ"
     gene            complement(3610..4524)
                     /locus_tag="PLU36_07100"
     CDS             complement(3610..4524)
                     /locus_tag="PLU36_07100"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HMZ46553.1"
                     /translation="MKKILAFTVAATMLFTACNKFEKAPSGMPYKVEKGPRKQKLAHG
                     QLLKMHFEYTLKDSVISSTMGHIPVYFSLDTTRSNKYSFSEVMTDCYVGDKLFCTLSI
                     DSMVKFGYVEYNPIFKKGDFLNCTVEFIKVFKDEAEQEADFKLELDAEKKREEKTIKD
                     YATKHNLKAVYTQNGVGVVVETMGDTAFKADSGKQVSVYYSGKLMDSGKEFDSNVKNG
                     VKANPFIFTLDAKMVIPGWDEAIKYFAKGGKGKLLIPALMGYGAQGSAPVIPPYANLV
                     FDVEITDVTDAPKLQPTTPPITVQPVTK"
     gene            complement(4521..5531)
                     /locus_tag="PLU36_07105"
     CDS             complement(4521..5531)
                     /locus_tag="PLU36_07105"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HMZ46554.1"
                     /translation="MMQPIHLFYPQLNNPSKIIIVSHQKPDGDAMGSSLGLYHFLIQL
                     GHTVNVISPTNWANFLNWMPGVEHVIDFEKNQEKCNQLIQSANIIFCLDFNVLYRTKH
                     VAPLIEAVNCIKILIDHHQEPQVDAFTYGISNTAKSSTCEMVYDFIVDSGNASTLNIN
                     IATCLYTGTMTDTGSFRFPSTTASVHKMVAHFKEIGLEHAIIHDNIYDNFLENRLRFI
                     GFVLCNRLEVLYEYNTALMYVYKQDLYKYDIKTGDTEGLVNYLLTIEGIKFGAIVIDR
                     EEERKWSFRSKGNFDVNTFARLHFEGGGHKNASGGRTDDNVEDTVKRFKQTIKQYEQE
                     LQ"
     gene            complement(5592..>6104)
                     /locus_tag="PLU36_07110"
     CDS             complement(5592..>6104)
                     /locus_tag="PLU36_07110"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ46555.1"
                     /translation="FTYTETFEIHAAHYGAKSVLGIDISENAVAQANKNAALNKLEHI
                     VHFKAMNAFDVLKVWAKEGKQYDVVMLDPPAFTKSRATLQKAITGYKEINLRGMKLVK
                     NGGFLVTSSCTNLVQPELFLHTIQLAAKDAGKKLKQLVFNAQASDHPIVWGLENTNYL
                     KFLIVQVCDI"
BASE COUNT         2209 a          851 c          970 g         2074 t
ORIGIN      
        1 gcaaagagtt taaataagat tatataaccg atttttgtat tgttgccaag tgtacaaact
       61 gtacaagtgt gcgacgcaac aaatgctgca aaatgttgaa atgttgattt taaaatttta
      121 attattgtaa tggaagataa attaaaaatg tttgagaacc gtttactaaa ggtatataag
      181 cataaaagca agcaggctaa aaggcaagaa gtatgttgtt atagagtgta cgaccatgat
      241 ttgcctgagt ttcctttttg cattgaattg tacgaagata aaatttattt agctgaatat
      301 gtaagaaggc atggtatgac tgatgaagag catgaagaat ggttagaaca atgtattgta
      361 ctaatttgta aattgctaaa tgtgcctgat gaaaatgttt ttgtaaaaca acgaaaaaga
      421 aaaagtaacc gattagatca atatgaaaaa acgggagaag aaaaaacatt tttcactgtt
      481 ttagaaaatg gattaaaatt tttagtaaat ctttctgatt atttagatac tggtttgttt
      541 ttagatcata gaattaccag aagcatggtt aaggaacaat gtgcaggtaa aagggtatta
      601 aatttatttg cttacactgg ctcctttagt gtgtatgctg cagcaggtaa tgctgcaagt
      661 gtaactactg ttgatttaag taaaacttat ttgcaatggg ccgaagataa tttcaccatt
      721 aatcatttta aagataaaaa caaatataac tttatacatg ccgatgtaaa gcaatattta
      781 aaaaccttgc agcccaatag ttttgatttg gtaattattg acccacccac atttagtaac
      841 agtaaaagaa tgaaagattt tttagacaca caaagagacc atgctgaatt attaaatgat
      901 atactctttg cattatcaga aaatggtgta atttatttta gcactaatta taccaaattt
      961 gtgatggata ctgaaaatat taaagcatct tatattaaag acataactaa agctactact
     1021 ccatttgatt ttgaaggaaa gctaaaaaga tggtgtttta agataggtaa ataaccgtta
     1081 taaatggtaa ttgaaattca taatgtttat ttatttttat aaaaaaaatt gatatgctaa
     1141 aatatttttt tgctttttgt attatttcca tttgtttttc ctcttgtttt atttttgggt
     1201 atagaaaaat aaaagggaac ggtatttata caactgaaaa acgcaattta tctgccaata
     1261 aaattaaagt ggaaggtaat ttagatatac aaattgcaca atctaacgaa ccaggtgtta
     1321 atataaaagc tgatgccaat ttattagaat atataataac tgaattagaa gatgaaagcc
     1381 ttgtaattaa aacaaaaaga aagtataagc ttgtatcgga aaacccaatt attgttgaag
     1441 tgaatacggc attattaaaa tctattagta ttgctggtaa aagcaatgta aaaggcttaa
     1501 ctaaatttga gggtggcgaa aaattagata ttaaaattgc tggtaagggt aatgtgcaac
     1561 ttgcagttaa cactcctaaa gtagaagtta aacttgctgg tgttggtgag ataaatttag
     1621 aaggtgaaac gaaagaagta attgtgaaaa tatcgggtag tggtaatttt aaaggcaaaa
     1681 atttaaaagc agagaatgct actattaaaa ttgcaggtag tggagatgca tctgtttttg
     1741 cctcagagaa tttagatgtt aaaatatcgg gtagtggtaa tgtggaatat ataggaagcc
     1801 ctaatattga tcagaaaatt tcaggtagtg gtactattaa acctataaaa gagtaagaaa
     1861 gttatctaat aaataaaaag acacaacaat actctttaaa aattttgcta taaatattta
     1921 gttaactttt aaattaagct tacactcaac aagtatcttt gaggcattta attatttaat
     1981 cgtatgaagg tttttaaatt tggaggagct agtgcaaatt ctatagaaag gatacaaaac
     2041 attccagaaa tattacagca atatagtaca gagcaaattg taatggttat ttctgcaatg
     2101 ggtaaaacta ctaatgcact tgaaaaagtt gctgaggcct tttataatgg taacaatata
     2161 caagctttag atttatttga aattgtaaaa caacaccaca ttaatactgc taagtattta
     2221 atgattacta attataacaa ttgcataaat caattaaacg attattttac tgaagtggaa
     2281 tggttactac atgataaacc tacgcagcaa tacgattact attatgatca aattgtttgt
     2341 attggtgaaa tgatgagtac ttgtgtgatt agttgctatt ttactgaaat aggcattggt
     2401 aatacctttt tagatgttag agatatttta aaaacagatg ataattttag agatgctgca
     2461 gtaaattggg agaaaacaac tgccaactta aaagagattg tattgcatcc aagccttaca
     2521 attattcaag ggtttattgg aagtacagac gaaaaccaga gtaccacact tggtagagaa
     2581 ggtagcgatt atactgcagc tatttttgcc aatttattac atgcagatag tttaactatt
     2641 tggaaagatg tagaaggtgt gatgaatgct gaccctaagg aatttgaaga tgcacagtta
     2701 attaatgaac taagttttaa tgaagtaatt gaaatggcat attatggagc ccaggttatt
     2761 catcctaaaa caataaaacc actacaaaat aaaaatattc cattgtatgt taaatctttt
     2821 ttagatagta gtttaaaagg aacaattatt tctaaacaaa ataataaaaa cttacctcct
     2881 attattgtga taaaaaataa tcaggtttta atgcacttgc atacacagga ttttagtttt
     2941 gttggcgaag agccaatggc tgctttatat aaaatatttt ctacattaaa aattcagcca
     3001 aacttaattc aaacaggtgc aataagtgtt caaatatgtt tagataataa agaagataaa
     3061 attgaacaac ttgcaattaa cgcagcaagt ttatttgaag tgaaagtaga aaaagagctt
     3121 cagttattaa caataaggca ttttacagaa gaacttaaaa acaaaatgct acacaataaa
     3181 aatgttattt tactacaaca aactaaacaa accttgcaag ctttatacac tatagaataa
     3241 taaacttgca aaaagttatt gaattgttta tgtattttta tgttataatt ttaacctatg
     3301 gcccaatata gtttaggcga tgcaatgaaa aaatttatta agcaaagtaa tttgcgtaat
     3361 gggcttagag cagtgcaaat tgaagaagta tgggaagaag taatgggcaa aacaattgca
     3421 aaatacaccg ataagattga aattgtaaat caaacacttt ttattaaaac gcaaataggc
     3481 tctttaaaga acgaattaat gtaccaaaaa ccattaataa ttgaaagaat aaatgagaag
     3541 tttggagaaa aaataattgc tactgtagtt atacaatagc aattacttta ataggcaata
     3601 attatatttt tattttgtta caggttgtac tgttataggt ggtgttgtag gttgcaattt
     3661 tggggcatca gtaacatctg taatttctac atcaaaaaca aggttggcat aaggaggaat
     3721 aactggagca gagccttgtg caccatagcc cataagagct ggtattaata attttccttt
     3781 accaccttta gcaaaatatt taatggcttc atcccatcct ggaataacca ttttagcatc
     3841 tagggtaaaa ataaaaggat tagcttttac accatttttt acattactat caaactcttt
     3901 accagaatcc attaatttgc ctgaataata tactgatact tgtttaccag aatctgcttt
     3961 aaatgctgtg tcacccatag tttcaacaac tacacctact ccattttggg tataaactgc
     4021 ttttaagtta tgtttagttg catagtcttt tattgttttt tcttcccgtt ttttttctgc
     4081 atctaattct aatttaaaat cggcttcttg ttctgcttca tctttaaaca ctttaataaa
     4141 ctctactgta caatttaaga aatcaccttt cttaaaaatt gggttatact ctacataacc
     4201 aaattttacc atactatcaa tactaagtgt acaaaataat ttatctccaa catagcaatc
     4261 tgtcattact tcggaaaagc tgtatttatt gctacgagta gtatctaaag aaaaataaac
     4321 aggaatatga cccattgtac tgcttattac actatctttt aaagtgtatt caaaatgcat
     4381 ttttaaaagc tgaccatgag ccaatttttg ttttcttggt cccttttcaa ctttataagg
     4441 catgccagat ggggctttct caaacttgtt acaagctgta aacaacatgg ttgcagctac
     4501 tgtaaatgct aaaatttttt tcattgtaat tcttgttcgt attgttttat tgtttgttta
     4561 aatcgtttta cggtatcctc aacattatca tctgttctac cacccgaagc atttttgtgt
     4621 ccaccacctt caaaatgcaa tcttgcaaaa gtgttcacat caaaatttcc tttacttcta
     4681 aaactccatt ttctttcttc ttctctatct ataacaattg ctccaaattt aattccttca
     4741 atggttaata aataattcac taagccttct gtatctcctg ttttaatatc atatttatat
     4801 aaatcttgct tatacacata cattaaggca gtgttatact catataatac ttctaatctg
     4861 ttgcataaaa caaaaccaat gaatcgcaat ctgttttcta aaaagttatc gtaaatatta
     4921 tcgtgaatga tagcatgctc taatccaatt tctttaaaat gtgcaaccat tttatgtacc
     4981 gatgcagtgg tagaaggaaa cctgaaggaa ccagtatctg tcatagtgcc tgtatataaa
     5041 caagtagcaa tatttatatt taaggtagaa gcattgccgc tatctactat aaaatcatat
     5101 accatttcgc aggtgctgct tttggctgta ttgctaatgc cataagtaaa agcatcgact
     5161 tgtggctctt gatgatggtc tattaaaatt ttaatacaat taacagcttc aatcaatgga
     5221 gctacatgtt tagtgcgata cagtacatta aaatctaaac aaaaaataat gttagcagat
     5281 tggattaact ggttacattt ttcttgattt ttttcaaaat caatcacatg ttctacacct
     5341 ggcatccagt ttaaaaagtt agcccaattg gtgggtgaaa ttacatttac agtatgccca
     5401 agttgaatta agaaatgata caaacctaaa gagctaccca ttgcatcacc atctggtttt
     5461 tgatgactta ctataataat ttttgaagga ttatttagtt gagggtaaaa tagatgaatt
     5521 ggttgcatca taaatggttg caaatgtaat ggaattaact attatacaaa taactgcata
     5581 aattacattt cttaaatatc acaaacctga actattaaaa attttaaata attggtattt
     5641 tctaaacccc aaacaattgg gtgatcggag gcttgagcat taaaaaccaa ttgttttaat
     5701 ttttttcctg catcttttgc agctaattga atggtatgta aaaataattc gggttgtact
     5761 agattggtgc agctactagt tactaaaaaa ccaccatttt ttacaagttt catacctctt
     5821 aaattaattt ctttataacc agtaatagct ttttgtaaag tagctctgct ttttgtaaat
     5881 gctggagggt ctagcattac aacatcatac tgtttgcctt ctttagccca tacttttaat
     5941 acatcaaatg cattcatggc tttaaaatgt acaatatgct ctaatttgtt taaagcagca
     6001 tttttattgg cttgtgcaac agcattttcg ctaatatcaa ttcctaaaac agattttgca
     6061 ccataatggg ctgcatgaat ttcaaatgtt tctgtataag taaa
//
LOCUS       DAOQPK010000133         6095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_7597_length_6095_cov_4.420530, whole genome shotgun sequence.
ACCESSION   DAOQPK010000133 DAOQPK010000000
VERSION     DAOQPK010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6095
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7597_length_6095_cov_4.420530"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="PLU36_07115"
     CDS             <1..812
                     /locus_tag="PLU36_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HMZ46556.1"
                     /translation="GNNGKISVSLQMNPNIQIWAIDVFGSLLTKYFRTGEIDMNEVHP
                     YISEGFGEDFVPENYDMSVIDHFEQVTDKDGAVMARKLAKEEGIFCGYSAGSCLQGLL
                     QLKNKLKKDDVVVCIFHDHGSRYVGKIYNDQWMMERGFLDVKSFKDIVSGRVGKKLIT
                     VQENDTVASAVELMKKYDIEHIPVFDNENIKGSISEAGLFLKVFNNAAIKDALIKDVM
                     ESAFPIVEFNTPVEKLSNLITKENGAVLAKDETTGDYHIVTKYDVLQSLAK"
     gene            954..1808
                     /locus_tag="PLU36_07120"
     CDS             954..1808
                     /locus_tag="PLU36_07120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HMZ46557.1"
                     /translation="MGDCKEQLKFLPDNSVDLIVTSPPYADQRKNTYGGIHTDKYVEW
                     FLPISEQLLRVLKPTGTFVLNIKEKVVEGERSIYVMELILEMRKQGWLWTEEFIWHKK
                     NCYPGKWPNRFRDAWERLLQFNKDKKFNMYQEEVMVPMGDWAKSRLKNLSNTDKIRDN
                     SKVGSGFGKNISNWLDRDKAYPTNVLHLATECNNKNHSAAFPEELPEWFIKLFTKKYD
                     TVLDPFMGSGTTLTVANRMIRNSIGIEIMPEYFEMVKKQFNTIELTLFEPTVEYAKTK
                     SERRYTVR"
     gene            1768..2520
                     /locus_tag="PLU36_07125"
     CDS             1768..2520
                     /locus_tag="PLU36_07125"
                     /inference="COORDINATES: protein motif:HMM:NF025864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmeII family type II restriction endonuclease"
                     /protein_id="HMZ46558.1"
                     /translation="MQKLNLKDVTRYVENNIGIFHQKRIQSLDSLKLSQVLRRKNPYL
                     FKAKFVLTAEQIIRGIVDAHISSNEETIFGDWLEGLAIFINGKVYGGYKSGITGLDLE
                     FDNNNIRHIVTIKSGPNWGNSSQIAKMVADFKTAKKALRTSNSQLNIVAVNGCCYGRD
                     NQPDKGDYFKYCGQRFWEFISGNTSLFTEIIEPLGHKAKEKNDEFVKSYSQMINKFTK
                     QFSNEFCNDYGDIEWEKLVKFNSATNEPKTKK"
     gene            2597..3058
                     /locus_tag="PLU36_07130"
     CDS             2597..3058
                     /locus_tag="PLU36_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46559.1"
                     /translation="MKKIIFLAASSIILWSCKNNAENNKPTIDTTIQEKQQETHNETS
                     EVIELNNGNKWLINIEMKPFITEGQKLVAEFLKTNNTDYKSLAKSLIEQNNLLIKSCT
                     MTGKSHDELHKWLEPHLKLVEDLEATTDATKAKEIVSKIEQSYQSFGNFFE"
     gene            3071..3631
                     /locus_tag="PLU36_07135"
     CDS             3071..3631
                     /locus_tag="PLU36_07135"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HMZ46560.1"
                     /translation="MKNNFTKLHRLLHWVTALAMMILFITGFLRMNWMSKTTIANAIN
                     YKTTDAPLTKEQIGGIIKTIREPMWQWHEIFAKVMIITLIVRIIYMVLKGIKFTNPFK
                     TNINFKEKIQGLTYVYFYVFVTISAFTGICLENGFFATYEDAIEDIHKWGLYWFPIFM
                     ILHIGGVYSAEHSNKKGITSKMISGE"
     gene            3718..4377
                     /locus_tag="PLU36_07140"
     CDS             3718..4377
                     /locus_tag="PLU36_07140"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HMZ46561.1"
                     /translation="MLPYQEIIIRILLASFLGALIGLERERKHWAAGLRTHMMVCVGS
                     CLIMMVSAFGFKDILGTPNVTLDPSRIAAQVVSGIGFIGAGTILLLKQGTIRGLTTAS
                     GLWTVAAIGLATGGGMYFAAVFVTIIAIIILWALKPLELNYFKKKNKTLRIITSLNEE
                     NKQLLNKLISTSIITNLQFEKIEDEYIYILEFENDHSEEIEKIINQIKNFPFIKQVSW
                     S"
     gene            complement(4420..5646)
                     /locus_tag="PLU36_07145"
     CDS             complement(4420..5646)
                     /locus_tag="PLU36_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMZ46562.1"
                     /translation="MNTKTLIVIGGGAAGFFCAINAARYNKNLKIIIVEKTNKLLSKV
                     KVSGGGRCNVTHDCYEINELIKHYPRGNNFLKKAFHWFNTTHTLEWFKAEGIELKKEE
                     DGRMFPKTNSSQTIIDCLLHLANYYNIEILLSSDVIKINKLNDLFTLQLANNKVLNCN
                     FLCIATGGYSKALQFKWLEDIGHTIEKPVPSLFTFNIPNNTITTLMGISVEYATIKII
                     GTKFQYSGPLLITHWGISGPAVLKLSAFAAQELNLKNYQFKIQINWLKDWTEQSLRED
                     WNSFREQYSSNKIYNKNPFQLPNRLWQFILQQSSIKEDAQWANLLSKQQNCLIKNLTA
                     MDLEVNGKTTFKEEFVTCGGIKLNEIDVNTMQSKIIPHLFFAGEVLNVDGITGGFNFQ
                     NAWTTGFIAAKGIAES"
     gene            complement(5718..>6095)
                     /locus_tag="PLU36_07150"
     CDS             complement(5718..>6095)
                     /locus_tag="PLU36_07150"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HMZ46563.1"
                     /translation="SDLNRDCSWDVLKNNGYERVETVDIDHMWSAVQFKRNLELPIVQ
                     PEPKIESAIAFEKSLIHYPIELERIFAKHKNVKDFFVSLPTTNQKEYLSWIQAAKRAD
                     TRQRRLDVVLEKLMAGKKKPSEK"
BASE COUNT         2245 a          873 c          963 g         2014 t
ORIGIN      
        1 ctggaaataa tggaaaaatt tctgtatcac ttcaaatgaa ccccaatatt caaatttggg
       61 caattgatgt atttggaagt ttacttacaa agtattttag aactggcgaa atagatatga
      121 atgaagtaca cccatacatt agcgaaggat ttggagaaga ttttgtacca gaaaattatg
      181 atatgagtgt gattgaccat tttgaacaag ttacagataa agatggtgct gtaatggcca
      241 gaaaattagc taaagaagaa ggcatttttt gtggttatag tgcaggcagt tgcttacagg
      301 gtttactaca attaaaaaat aaactaaaga aagatgatgt agttgtttgt atttttcatg
      361 accatggcag ccgttatgta ggaaaaattt acaacgacca atggatgatg gaacgcggct
      421 ttttagatgt aaaatcattt aaagatattg taagcggaag agtaggtaaa aaattaatta
      481 cagtgcaaga aaatgatacc gttgcatctg ctgtagaatt aatgaaaaaa tacgacattg
      541 agcatattcc tgtttttgac aatgaaaata taaaaggcag tataagtgaa gcaggtttat
      601 ttttaaaagt atttaataat gcagccatta aagatgcttt aataaaagat gttatggaat
      661 ctgcttttcc tatagttgaa tttaatacac ctgtagaaaa attaagcaat cttattacaa
      721 aggaaaatgg tgctgtttta gccaaagacg aaactacagg cgattatcat attgttacca
      781 aatatgatgt attacaaagt ttagcaaagt aaaagtagaa ttaaaaaata gttgcatgta
      841 aaactacctc aattatcaac ttttaaacat ttattggcag aaaaaacata aaaacttgta
      901 aattcactaa aataattagg ataaagaaaa tggaattgac aacagacttt tatttgggtg
      961 actgcaaaga gcaattaaag tttcttcctg acaattcggt ggatttaatc gttacttcac
     1021 caccctatgc cgaccaacgt aaaaatactt atggtggcat acatactgat aaatatgttg
     1081 aatggtttct gccaatatcc gaacaacttt tacgtgtact aaaaccaaca ggaacttttg
     1141 tactaaatat caaagaaaaa gtagttgaag gcgaaagaag catctatgtg atggagttaa
     1201 ttcttgaaat gagaaaacaa ggttggttat ggacagaaga gtttatttgg cacaagaaaa
     1261 attgttaccc tggcaaatgg ccaaacaggt ttagagatgc ttgggaaagg ttgcttcaat
     1321 tcaacaagga taaaaagttc aatatgtacc aagaggaagt aatggtacct atgggagatt
     1381 gggcaaaatc tagacttaaa aacttatcaa ataccgacaa aatacgagac aactcaaaag
     1441 ttggaagtgg ttttggaaaa aatatttcta attggttgga cagagataaa gcatatccaa
     1501 caaatgtttt gcatttagct acagaatgta ataataaaaa ccacagtgca gcttttccag
     1561 aagaattacc tgaatggttt attaaacttt ttaccaaaaa atacgataca gtgcttgacc
     1621 cttttatggg ttcaggaaca acattgactg ttgcaaacag aatgatacga aattctattg
     1681 gaatagaaat aatgccagaa tattttgaaa tggtaaaaaa gcaatttaat acaatagaat
     1741 taaccctatt tgagccaacg gtagaatatg caaaaactaa atctgaaaga cgttacacgg
     1801 tacgttgaaa ataatattgg aatttttcac caaaaaagaa ttcaaagcct tgatagcttg
     1861 aaactctctc aagtgctgag aagaaaaaat ccttatttgt tcaaagcaaa atttgtactt
     1921 acagcagaac aaattattag aggaatcgtt gatgctcata tttcttcaaa tgaggaaact
     1981 atttttggtg attggttaga aggacttgcc atttttataa atggaaaagt atatggtggt
     2041 tataaatcag gaattacagg acttgattta gaatttgata ataataacat tagacatatt
     2101 gttacaatca aatcaggtcc aaattgggga aacagttcac aaattgctaa aatggttgct
     2161 gactttaaaa ctgcaaaaaa agcattacga acaagtaact cacaattaaa cattgttgct
     2221 gtaaatggtt gttgttatgg cagagataat cagccagaca aaggcgatta tttcaaatat
     2281 tgtggacaaa ggttttggga atttatttcg ggtaacacca gcttatttac agaaataatt
     2341 gaaccacttg ggcacaaagc aaaagagaaa aatgatgaat ttgtaaaatc gtattctcaa
     2401 atgataaaca agtttacgaa acaattttca aacgaatttt gcaacgacta tggagatata
     2461 gaatgggaaa agttagtgaa atttaattcg gcaacaaatg aaccaaagac aaagaaataa
     2521 gaaactcctt cctctaacaa tgtatttata cataacttat ctttacacca attcatttta
     2581 aaaccttttt atcatcatga aaaaaataat tttcttagcg gcaagtagta ttattttgtg
     2641 gagttgtaaa aacaacgcag aaaataataa acctactata gatactacca tacaagaaaa
     2701 acaacaagaa acacacaacg aaacttctga agtaatagaa ctaaataatg gtaataaatg
     2761 gctgataaat attgaaatga aaccttttat aacagaaggc caaaaattag tagctgaatt
     2821 tctaaaaacc aataatacag attataaatc tttagctaaa agtttaatag agcaaaataa
     2881 tttattaatt aaaagctgca ccatgacagg caaaagccac gatgaattac ataaatggtt
     2941 agagcctcat ttaaaacttg tagaagattt ggaggcaact acagatgcaa caaaagcaaa
     3001 agaaattgtt tcaaaaattg aacaatctta tcaaagcttt ggtaactttt ttgaataata
     3061 aatttataat atgaaaaata atttcaccaa attacatcgt ttgttacatt gggttactgc
     3121 attggctatg atgattttgt ttattacagg ttttttaaga atgaattgga tgagtaaaac
     3181 caccattgca aatgcaatta attacaaaac aaccgatgca cccttaacta aagaacaaat
     3241 tggtggaata attaaaacta taagagaacc tatgtggcaa tggcacgaaa tatttgccaa
     3301 agtaatgatt ataactttaa ttgttagaat tatatatatg gttttaaaag ggataaaatt
     3361 tactaatcca tttaaaacaa atattaattt taaagaaaaa attcaaggct taacttatgt
     3421 ttacttttat gtgtttgtca ctatatctgc atttacaggt atttgtttag aaaatggttt
     3481 ttttgcaacg tatgaagatg ccattgaaga tattcacaaa tggggcttgt actggtttcc
     3541 tatttttatg atattacata ttggaggagt atattcagcc gaacattcta ataaaaaagg
     3601 tataacttct aaaatgataa gtggtgaata atgcatggct cttttactac ttgatttatt
     3661 agcataattt ttgtatccta ttttggagta ttcactttat tcaaaactcc aaactttatg
     3721 ttaccttatc aagaaataat tattcgtatt ttacttgctt catttttagg tgctttaatt
     3781 ggtttagaaa gagaaagaaa acattgggca gcaggcttaa gaacacacat gatggtatgt
     3841 gtaggttctt gtttaataat gatggtttct gcttttggat ttaaggatat tttaggcaca
     3901 ccaaacgtta cactcgaccc ttctagaatt gcagcacaag ttgttagtgg aattggtttt
     3961 attggtgcag gaactatttt attattaaag caaggtacaa tacgtggact aacaacagcc
     4021 tctggtttat ggacagtggc tgctattggc ctagcaacag gcggtggaat gtattttgca
     4081 gcagtatttg ttacaattat tgctattatt attttatggg ctttaaaacc tttagaacta
     4141 aattatttta agaaaaaaaa taaaacacta agaattatta caagcttaaa cgaagaaaat
     4201 aagcagctac ttaataaact tataagtaca tctataatca ccaacctaca atttgaaaaa
     4261 atcgaggatg aatatattta tattttagaa tttgaaaatg accactcaga agagattgaa
     4321 aaaataatta accaaattaa aaatttccct tttataaaac aggtttcatg gagttaatta
     4381 tccaatttta aaaaattaca aaccagtaaa tagtataaat taactttctg caattccctt
     4441 tgctgcaata aagccagttg tccatgcatt ttgaaaatta aaacctcctg taataccatc
     4501 tacatttagt acttctcctg caaaaaacaa atgtggtata attttacttt gcattgtatt
     4561 cacatcaatt tcgtttaatt taataccacc acaggttaca aactcttctt taaaagtagt
     4621 tttaccattc acctctaaat ccatggcagt aaggtttttt attaaacaat tttgttgttt
     4681 acttaataaa ttggcccatt gtgcatcttc cttaatacta ctttgttgaa gtataaactg
     4741 ccataatcta ttgggtaatt gaaaaggatt tttattataa attttatttg aggaatattg
     4801 ttcacgaaag ctattccaat cttctcttaa ggattgttct gtccaatctt tcaaccaatt
     4861 aatttgaatt ttaaattgat aatttttaag gtttaactcc tgagcagcaa aagcagataa
     4921 ttttaaaact gctgggccac ttatacccca gtgagttatt aacaatggtc cactatattg
     4981 aaattttgtg ccaataattt ttatggttgc atactcaaca cttatgccca ttaatgttgt
     5041 tattgtatta ttaggaatat taaaagtaaa taaagatgga acaggttttt ctattgtatg
     5101 gcctatatct tcaagccatt taaactgcaa ggctttgcta taaccacctg ttgcaataca
     5161 taaaaaatta cagtttaata ctttattatt tgctaattgc agggtaaaca gatcattaag
     5221 tttatttatc ttaataacat cgctgcttaa caaaatttca atgttatagt aattggctaa
     5281 atgtaaaagg caatctataa ttgtttgaga actgttggtt ttaggaaaca ttctaccatc
     5341 ttcttccttc ttcaactcta ttccttcggc tttaaaccat tctaaagtat gtgtagtatt
     5401 aaaccaatga aatgcttttt ttaaaaaatt attacctctt ggatagtgtt ttattaattc
     5461 atttatttca taacaatcat gtgttacatt gcatcttcca ccaccactta cttttacttt
     5521 acttagcagt ttattcgtct tttctacaat aattatttta agatttttat tatatctagc
     5581 agcattaatg gcacaaaaaa aaccagcagc accgccacca ataacaatta aggtttttgt
     5641 attcataact aattatacaa atctacaagg tataatagca aaaaaaaaca ccttacaatt
     5701 tgtaaggtgt tttggggtta tttctcagaa ggcttttttt tgcctgccat taatttttct
     5761 aaaactacat ccaatcttct ttgtcttgta tctgcccttt tagcagcttg tatccaagat
     5821 aaatattctt tttggttggt agtaggaagc gatacaaaaa aatcttttac atttttatgt
     5881 tttgcaaaaa ttctttcaag ctcaattggg tagtgtatta aacttttttc aaaagcaatt
     5941 gcactttcaa ttttaggttc aggttgtaca attggtaatt ctaaatttct tttaaattgt
     6001 acagcactcc acatatgatc tatatctaca gtttctacac gttcgtaacc attgtttttt
     6061 aatacatccc aactgcagtc tctatttaaa tcgct
//
LOCUS       DAOQPK010000134         6084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_7616_length_6084_cov_4.647869, whole genome shotgun sequence.
ACCESSION   DAOQPK010000134 DAOQPK010000000
VERSION     DAOQPK010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6084
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7616_length_6084_cov_4.647869"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..2647
                     /locus_tag="PLU36_07155"
     CDS             188..2647
                     /locus_tag="PLU36_07155"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HMZ46564.1"
                     /translation="MRIVPFIISAAITTGLIITLNTQLKIGTSKTPKLGYFLSPQYGF
                     WQNAEPTDIAFNDDIKMPGVTNQVDVYFDERLVPHVYAENDADAYFIQGYLHAKFRLW
                     QMEFQTHIAAGRLSEIVGEDKIATDKYFRRLGMVYGAENTLKAIDKDAETKAACDAYT
                     AGVNTYINSLKPEQIPFEYKLLDYKPEAWTNLKTAIFLKFMSWDLAGKTEDLLFTNAK
                     TYFGYDDLQKLYPLIQDTLDPIIPKGTVYEKASITVTKPANSDSLYLERKDTINNTPT
                     VKPNKNNGSNNWAVAGIKTKSGKPILCNDPHLGLNLPSLWFEMQITTPKYSSYGATFP
                     GSPAIIIGFNDSCAWGVTNAGRDVEDFYEIKFRDSTMQEYFFDGRWQAAQIRTETIKI
                     KGKPAIEEKIAMTIFGPVMYDKSYPAKSNKEINIAVRWKAHDESNELRTFYKLNRMNN
                     YLDYVDAISTYECPGQNFVFAAHNGDIALRQQGAFAAKWQQQGDFIMPGTDSSYMWQG
                     IIAAKENPQIVNPQRGFVSSANQQATDATYPYYLGRPNLFPPYRGFIINRKLAAMNNI
                     TPQDMQALQTDNYNVYAEICKPVLLKYINSNELKDNEKKYLQIFTSWNLRNDIHEKGI
                     TVFKIWWDSLETATWADEFEKSTLKLPWPDESTLIEKIINDSSNYKFIDNINTKNKKE
                     TLAEIVTQAFKNASKTLDSLEKINKLEWVNYKDTKVQHLLQLPALSKLHLPIGGGVHI
                     INATSTTHGPSWRMIVHLTDKIEAYGVYPGGQNGNPGSKYYSNFVDTWAAGKYYNIIF
                     ISKQEARKDTRMKWHTVFVNG"
     gene            2658..3008
                     /locus_tag="PLU36_07160"
     CDS             2658..3008
                     /locus_tag="PLU36_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46565.1"
                     /translation="MKFITALVLTALLCFAIGLFTNLPWYSFVLCSLIVAVAIHQKPF
                     KAFLAGFIAAFLLWFIMAWLKDSANEHLLSKKVAEILPLNGNYHLLIILSGLIGGLLA
                     GMGALTGSFLRKIK"
     gene            3088..3819
                     /locus_tag="PLU36_07165"
     CDS             3088..3819
                     /locus_tag="PLU36_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584500.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HMZ46566.1"
                     /translation="MKNYLPHFIIAIAIIIFGFIISNAYIYKFTSTETIVVTGLAEKD
                     FTSDLIVWKGSFTRTGLDLKEVYAQIKQDEASIKNYLKEKGLPDSSVIFSSFDVLKNY
                     QSKYAENGTITGNIFTGYTLTGEVKIESMNLPLVEKISREVTELLQKGIEFNSQKPSF
                     YYTKLHDLKIDLLANASKDAKLRAETIAKNSGTNINSLKRATSGVFQITGKNDNEEYS
                     YGGSFNTTSKFKTASITIRTEYLCK"
     gene            3827..4204
                     /locus_tag="PLU36_07170"
     CDS             3827..4204
                     /locus_tag="PLU36_07170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HMZ46567.1"
                     /translation="MKLFSTLKMSYHIYGLKNCNTTQKAIQWFKQHKLAYKFINNRTE
                     PISKSKLELWCNQVSWEKLLNKKGTAFKLLHPAIQQSATTQEKAIEIMLARPSTIKRP
                     LIEKDNKIITLGFSQVFFEIHYL"
     gene            complement(4215..4868)
                     /locus_tag="PLU36_07175"
     CDS             complement(4215..4868)
                     /locus_tag="PLU36_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HMZ46568.1"
                     /translation="MIEAIISGLTLGLLLSISVGPVIFAVIRQSLSNGHIGGYAFIAG
                     VSASDVTLVLVCNVLSTIFNHLLANKMIIGVAGSCFLIAIGIYTFFFKKVVFSNENSG
                     TEKKFKKTELAGIFLSGFFMNLLNPGVFIFWLAATAKIHAQAALQLHSLRYIVTVFFI
                     CLAVVLATDIAKVLLAGKIRSKLTPHNIHIINKISGVIFIVFGMFLIWGVMFHGDKI"
     gene            complement(4931..>6084)
                     /locus_tag="PLU36_07180"
     CDS             complement(4931..>6084)
                     /locus_tag="PLU36_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HMZ46569.1"
                     /translation="MIKQLLSLRGYENIPVKSIGKGWLTLGGSTELQKNAGYYAGDLS
                     GITQKKAPFWFQPFYKPGKKIAKEKDWQLKLEEIVRKAPEWDIGFIIGVPAWIQMLLE
                     MIIEKYQLKNIHEIWPNLAFFVHGGVSFEPYKRGFEKLLGKPINYIETYLSSEGFLAY
                     QNRQNAKGMRLVTSEHIFMEFIPFNEKNFDSEGEITTNPETLMIHEVEEGKDYALMIS
                     TSAGTWRYLIGDTIRFVDKERSEIIITGRTKHFLSLVGEHLSVENMNKAIKLVGEEFN
                     ISIPEFTVAGVPYQSFFAHHWYIACNDKIDADTLALKIDEHLKVLNDDYAVERKSALK
                     EIFLTILTEDKFMQFMKLKGKIGGQHKFPRVLKGKMLEDWHLFLNDELK"
BASE COUNT         2247 a          937 c          897 g         2003 t
ORIGIN      
        1 ttccaagccc tagagcaagc gctggttgcg gcaacgaaga aaaagtttta cgacctaaat
       61 tcccattatt tgtggcacaa aaaatttatg caggaataaa agatttttta aaacaaagtg
      121 ccgatgatta aaattcattt agaagcatta aaataaatta actattttca gactttaaat
      181 aaatattatg cgtattgttc cttttataat ttcagcagca atcactacag gcttaattat
      241 tactttaaat acacaattaa aaattggtac tagtaaaaca cccaaacttg gttattttct
      301 ttcgccccaa tatggatttt ggcaaaatgc cgaacctaca gacattgcat ttaacgatga
      361 tattaaaatg ccaggtgtta ccaatcaggt tgatgtttat tttgatgaaa gattagtgcc
      421 tcatgtatat gcagaaaacg atgcagatgc ctattttata caaggatatt tacatgccaa
      481 atttaggtta tggcaaatgg aatttcaaac tcatattgca gcaggtaggt taagtgaaat
      541 agttggagaa gacaaaattg caaccgacaa atattttaga agattaggta tggtatatgg
      601 tgctgaaaat actttaaaag caattgataa agatgcagaa acaaaagcag cttgtgatgc
      661 ttatacagca ggtgttaata cttatataaa ctctttaaag cccgagcaaa ttccttttga
      721 atataaatta ttagactata agccagaagc ttggactaat ttaaaaacag ccatcttctt
      781 aaaatttatg agttgggatt tagcaggaaa aactgaagac ttattattta caaatgctaa
      841 aacttatttt ggatatgatg atttacaaaa actctatcca ttaattcaag atacattaga
      901 ccccatcatt cctaaaggca cagtgtatga aaaagcatct attactgtaa ctaaaccagc
      961 taatagcgat agcttatatt tagaaagaaa agatactatt aataacaccc caacagtaaa
     1021 acctaataaa aataatggta gcaataattg ggcagtagct ggcattaaaa caaagagtgg
     1081 aaaaccaatt ttatgtaatg atccacatct tggcttaaat ctcccctcac tttggtttga
     1141 aatgcaaatt actactccaa aatatagtag ctatggagct acttttcctg gctcgcctgc
     1201 cattataatt ggttttaatg atagttgtgc atggggtgta actaatgcag gaagagatgt
     1261 agaagatttt tatgaaataa agtttagaga tagtacaatg caagaatatt tttttgatgg
     1321 cagatggcaa gcagcacaaa taagaacaga aactataaaa attaaaggta agccagccat
     1381 agaagaaaaa atagcaatga caatttttgg acctgttatg tacgataaat cctatcctgc
     1441 aaaatctaat aaagaaatta atattgcagt gagatggaaa gcacacgacg aaagtaatga
     1501 gctaagaact ttttataaac taaatagaat gaacaattat ttagattatg tagatgccat
     1561 ttcaacctat gaatgcccag gacaaaattt tgtatttgca gctcacaatg gcgatatagc
     1621 tttaaggcaa caaggtgcat ttgctgcaaa atggcaacaa caaggagatt ttataatgcc
     1681 aggtaccgat agctcttaca tgtggcaagg cattattgca gcaaaagaaa accctcaaat
     1741 tgttaatccg caaagaggtt ttgttagtag tgccaaccaa caagcaacag atgctacata
     1801 tccatattat cttggtcgtc ctaatttatt tcctccttat cgtggcttta ttattaacag
     1861 aaaacttgca gcaatgaata atattacacc tcaagatatg caggcactac aaacagataa
     1921 ttataatgtt tatgcagaaa tatgcaaacc ggttttatta aaatatatta attccaacga
     1981 actgaaagat aatgaaaaaa aatatttgca aatatttact tcctggaatt taagaaatga
     2041 tattcatgaa aaaggtatta cagtttttaa aatttggtgg gatagtttag aaacagcaac
     2101 atgggcagat gaatttgaaa aatctacttt aaaattgccc tggcctgatg aaagtacttt
     2161 aatagaaaaa ataataaacg atagcagtaa ttataagttt attgataata ttaatacaaa
     2221 aaataaaaag gaaaccttag cagaaattgt tacacaagca ttcaaaaatg cttctaaaac
     2281 tttagacagt ttagagaaaa taaataaatt agaatgggtg aactataaag ataccaaagt
     2341 acagcactta ttacaattac ctgcattaag caaattacat ttgcccattg gtggtggtgt
     2401 tcatattatc aatgcaactt ctaccacaca tggcccaagt tggagaatga ttgtacattt
     2461 aacagataaa atagaagcct atggcgttta tcctggtggg caaaatggaa acccaggaag
     2521 taaatattac agtaattttg tagatacttg ggcagccggt aaatactata atattatttt
     2581 tatttcaaag caagaagcaa gaaaagatac aagaatgaaa tggcatacag tttttgtaaa
     2641 cggataaaat ttaaacaatg aaatttatta cagcattagt tttaacagca ttattatgtt
     2701 ttgcaattgg cttatttaca aacttaccct ggtatagttt tgtgttatgt agtttaattg
     2761 tggcagttgc cattcatcaa aaaccattta aagcattttt agcaggtttt attgctgcat
     2821 ttttattatg gtttataatg gcttggttaa aagactctgc caatgaacat ttactttcta
     2881 aaaaggttgc tgaaatttta cccctcaatg gcaattatca tttattaata atactatcag
     2941 gattgattgg tggtttatta gctggcatgg gagctttaac aggaagcttt ttaagaaaaa
     3001 taaaataatt aaaagttaaa gtaaaagtat attagagtaa tttatctatt tttaaatata
     3061 acttcatctt aaataaaatt aattataatg aagaattact taccgcattt tataattgca
     3121 atagctataa ttatttttgg ttttattatc agtaatgctt acatatataa atttacttct
     3181 accgaaacaa ttgttgtaac aggtttagca gaaaaagact ttacaagcga tttaattgtt
     3241 tggaaaggaa gctttaccag aactggatta gatttaaaag aagtatatgc tcaaataaaa
     3301 caagatgaag caagcattaa aaattatttg aaagaaaaag gactaccaga tagtagtgta
     3361 attttttcgt cttttgatgt attgaaaaac tatcaatcta aatatgccga aaatggaaca
     3421 attacaggca atatttttac tggatataca ctaactggcg aagttaaaat tgaatcaatg
     3481 aatttaccat tggttgaaaa aatttcgaga gaagtaactg aattattgca aaagggaatt
     3541 gaatttaact cgcaaaagcc atctttttac tatacaaaat tgcatgattt aaaaatcgat
     3601 ttattggcta atgcgtcaaa agatgcaaag ttaagagccg aaacaattgc caaaaactct
     3661 ggtactaata ttaatagctt aaagagagct acttctggcg tttttcaaat taccggaaaa
     3721 aatgataatg aagagtacag ctatggaggc tcttttaata ctacttctaa atttaaaact
     3781 gcctccataa ctattagaac agaatatttg tgtaaataaa tttttattga aattattttc
     3841 tactttaaaa atgagctatc atatttatgg tttaaaaaat tgcaatacca ctcaaaaagc
     3901 aatacaatgg tttaagcagc ataaattagc ttataaattt attaataacc gaacagagcc
     3961 aatatccaaa agcaaattag agctttggtg taatcaggta agttgggaga agttattgaa
     4021 caaaaaaggt actgcattta aattgttaca tcctgctatt caacaatctg ctacaacaca
     4081 agaaaaagca attgaaataa tgcttgcaag gccaagtacc ataaaaaggc cattgataga
     4141 gaaggataat aaaattatta ctttaggttt tagccaagtt ttttttgaga tacattattt
     4201 atgagatatc tgtcttaaat tttatctcca tgaaacatga caccccatat taaaaacata
     4261 ccaaaaacaa taaaaattac accagaaatt ttattgatga tatgaatatt gtgtggtgta
     4321 agctttgacc ttatttttcc tgccaataaa acttttgcaa tatctgttgc taaaactaca
     4381 gctaaacaaa taaaaaaaac agttacaatg tatcttaaag aatgtagctg tagtgcagct
     4441 tgtgcatgta ttttagctgt ggctgctaac caaaaaataa atacacctgg attaagtaaa
     4501 ttcataaaaa agccagataa aaaaatgcca gctaattctg tttttttaaa ttttttttcg
     4561 gtaccagaat tttcatttga aaaaacaact tttttaaaaa agaaagtata aatacctata
     4621 gcaattaaaa aacaactacc tgctacacct ataatcattt tattagctag taaatgatta
     4681 aaaattgtac ttaatacatt gcaaactaaa actagtgtaa catcgctggc agaaactcca
     4741 gcaataaatg cataacctcc tatatggcca ttacttaagc tttgtctaat tactgcaaaa
     4801 ataaccggac caaccgatat ggaaagtagt aatcctaatg ttagtccact tataattgct
     4861 tctatcattt tatttatttc ctatataagt tttataagaa ttattgttac aaaaaattaa
     4921 tgtcaaaatt ttattttaat tcatcgttta aaaaaagatg ccaatcttct aacatttttc
     4981 cttttaatac tcttggaaat ttatgttgcc ctcctatttt tccttttagt ttcataaact
     5041 gcataaactt atcttcagta agtatagtta agaaaatttc ttttaatgca ctttttcttt
     5101 ccactgcata atcatcattt aatactttta aatgttcatc tatttttaaa gccaatgtat
     5161 ctgcatcaat cttatcattg caggcaatat accaatgatg agcaaaaaaa ctttgataag
     5221 gtacaccagc aactgtaaat tctggaatac taatgttaaa ttcttctcca actaatttaa
     5281 tagctttatt catattttct acactcaaat gctcccctac caggcttaaa aaatgttttg
     5341 ttcttccggt aattattatt tcacttcttt ctttatctac aaacctaatg gtatcgccaa
     5401 ttaaatatct ccatgtgcct gcagaagtgc taatcattaa tgcatagtcc ttaccttctt
     5461 ctacttcatg tatcattaat gtttctggat tggttgtaat ttctccttcc gaatcaaaat
     5521 ttttttcatt aaaaggaata aattccataa aaatatgttc actggttacc aacctcatac
     5581 cttttgcatt ttgcctattt tggtaagcta aaaatccttc gctagataaa taagtttcta
     5641 tataattaat tggtttacca agcaactttt caaaccctct tttgtaaggt tcgaaactta
     5701 cgcccccatg tacaaaaaat gcaagattgg gccatatttc gtgaatgttt tttaactgat
     5761 atttctcaat aatcatttct aataacatct gaatccaggc tggcacacca ataataaaac
     5821 caatatccca ttctggagct tttcttacaa tttcttctaa ctttaattgc cagtcttttt
     5881 ctttagcaat ttttttacca ggtttgtaaa atggctgaaa ccaaaatggt gctttttttt
     5941 gcgttattcc gcttaaatct cctgcataat aacctgcatt tttttgaagt tctgtactac
     6001 ctcctaatgt aagccaacct ttacctattg atttaactgg aatattttcg tatcctctta
     6061 aactcagcaa ttgttttatc atta
//
LOCUS       DAOQPK010000135         6058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_7672_length_6058_cov_4.801099, whole genome shotgun sequence.
ACCESSION   DAOQPK010000135 DAOQPK010000000
VERSION     DAOQPK010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7672_length_6058_cov_4.801099"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PLU36_07185"
     CDS             <1..374
                     /locus_tag="PLU36_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HMZ46570.1"
                     /translation="KALNIFDKVLENTPGIKIIYSSVKDFFEAFAGNKKKFDKPVLVN
                     VDANNVWRIGFITKSDATDFDLKDHLVVYVPHSYAISGITYFVPKSNVKELKNITATN
                     AMKFTVSGGVVDFDDVNDKAN"
     gene            complement(450..992)
                     /locus_tag="PLU36_07190"
     CDS             complement(450..992)
                     /locus_tag="PLU36_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HMZ46571.1"
                     /translation="MRAKHYLILLVLILTAASFTIYKQKKIYSFKVDGIEGGKINFAK
                     FKGKKILIVNTASECQYTYQYAELQSLYTTYQDKLVIVGFPCNQFGHQEMGSNDDINS
                     FCKVRYGVSFPLASKVDVKGENAAPIYKWLCNKSENGVMDVTIKWNFNKFLIDEKGRL
                     LAHFPSNVKADSDEILKYLK"
     gene            complement(1163..2569)
                     /locus_tag="PLU36_07195"
     CDS             complement(1163..2569)
                     /locus_tag="PLU36_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HMZ46572.1"
                     /translation="MKKLLSLFSICICFTAFIFTSCKKEGFITDSNALLRTSVDTLFY
                     DTVFTSVGSITQSFKIFNPNNKKLKLTSVELMGGNLSFYKMNVDGFSSSIVENIEINA
                     NDSIYVFAMVNINPTVTNLPFIIQDSIKISYNGNTRFVQLQAYGQNAIFLKNKLVTID
                     TTWNKDLPIVVVGALTVQPSVTLTIAKGTKVYFHANAPMLVQGTLKAIGEKDEPSKII
                     FTGDRLDEPYRDFPGGWPGIYFLDNSVNNVMQYCIIKNAYQGTIAQNPSSNANPKLIL
                     NECILDNIYDVAVGATNSSIEANNCLISNSGYNIYLTAGGNYNFTHCTVTSIGNYYLQ
                     HKYPVLNISNKVSETISNDLTANFTNCIVYGDGGIPEDEISIKKQGSTIFNVNFNNTL
                     YKLKNNEPSTANFNGNKLKNIPPDFDSININNRYYNFRLKPNAAAINKGTNTSLLTDL
                     DGNNRSIGLPDLGCYERQ"
     gene            complement(2686..2796)
                     /locus_tag="PLU36_07200"
     CDS             complement(2686..2796)
                     /locus_tag="PLU36_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HMZ46573.1"
                     /translation="MGRGDKKTAKGKRFKGSFGKSRPHKTKTNKAATKTA"
     gene            complement(2885..3154)
                     /locus_tag="PLU36_07205"
     CDS             complement(2885..3154)
                     /locus_tag="PLU36_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HMZ46574.1"
                     /translation="MNKAELIAQLADDAGITKTQANAALDSFVDTVTKTLKKGDKVTL
                     VGFGTFSVSKRAARTGRNPQTGETIKIKAKKVARFKAGKELSSKI"
     gene            complement(3455..3760)
                     /locus_tag="PLU36_07210"
     CDS             complement(3455..3760)
                     /locus_tag="PLU36_07210"
                     /inference="COORDINATES: protein motif:HMM:NF025479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HMZ46575.1"
                     /translation="MFIYNVTIQVEPSIHDAWLIWLQNIHIPEVMNTGCFTKYQLVKL
                     LDTKEEENTTYAVQYYLESKADYNRYIELFAPLLREAGLLAWGNKFAAFRTFMQVVN"
     gene            complement(3760..5805)
                     /gene="paaZ"
                     /locus_tag="PLU36_07215"
     CDS             complement(3760..5805)
                     /gene="paaZ"
                     /locus_tag="PLU36_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HMZ46576.1"
                     /translation="MQKLYNYILGKKITGDGDGQVLYNAVSGNAIATASTAGLNFDDI
                     LSYARNKGSALRKMSFHERGLMLRALANHLRKHLDKFYSISYQTGATKADSWIDIEGG
                     IGNLFANASLRRKLPDTPFCLDGEHHALGKQNTFMGHHLLVPKEGVAIHINAFNFPVW
                     GMLEKIAVNLLAGMPCVVKPATVTSYLTEAVVKEIMDSNILPNGALQLICGSAGNMLD
                     YVTSQDVVTFTGSATTGLMLKSHKRILEENVPFNMEADSLNCIILGNDVTPEMPEWDI
                     FIKEVRKEITVKCGQKCTAVRRIFVPENKIEAVQTALIKALSQTTIGNPLNEKVRMGS
                     LAGNTQRNEVKTQVQKLLASSQLIYGSLDSVETIDADANKGAFISPMLLLNTTPFLTN
                     EVHEVEAFGPVSTLMPYKNLEEAIELSKMGKGSLVSSIVTASEKNATNYVLGAGAYHG
                     RILVLNNECAKENTGHGSPLPLLVHGGPGRAGGGEEMGGLRGIKHYMQRVAIQGSPTM
                     LTAVSKVFQPNAKGNIDGRHPFTKYFEELQIGDQLITEKRLITSEDINKFADLSGDHF
                     YAHKTETDFSGTMFEQQVAHGYFIMSIAAGLFVDGFKKNPVLLNYGIDELRFTKPVYP
                     GAEIYIRFTCKEKIAQDTKDENDLPKGIVKWLVEIFDESNEITGIATILTMVAKKNK"
BASE COUNT         1974 a          952 c          843 g         2289 t
ORIGIN      
        1 gtaaagcatt aaatattttt gataaagttt tagaaaatac accaggtatt aaaattattt
       61 atagttccgt taaagatttt tttgaagctt ttgcaggtaa taagaagaaa tttgataagc
      121 cagtattagt aaatgtagat gcaaataatg tttggcgtat tgggtttatt acaaaatctg
      181 atgctactga ttttgatttg aaagatcatt tagtggtgta tgttccacat tcttatgcaa
      241 taagtggtat tacttatttt gtacctaaaa gtaatgtgaa agaattaaag aatataactg
      301 ctacaaatgc aatgaaattt acggtaagtg gtggtgttgt agattttgat gatgttaacg
      361 ataaggcaaa ctaaacttat tttttacttg cctaagctaa atgtttgatt gtttttgaat
      421 agtaaatttt gcatgttacc ttttaatatt tattttaaat attttaaaat ttcatcgcta
      481 tctgctttta cattactcgg aaaatgtgct aataatcttc ctttttcatc gattaaaaac
      541 ttattaaagt tccattttat ggttacatcc ataacaccat tttcgctttt attgcaaagc
      601 catttataaa tgggtgctgc attttctcct tttacatcta ccttgcttgc taaaggaaaa
      661 cttacaccat atcttacctt gcaaaaagag ttaatatcat cattgctacc catttcttga
      721 tgtccaaatt gattgcaagg aaatccaaca atcactaatt tatcttgata ggttgtatat
      781 aaagattgca actcggcata ttgataagtg tattgacatt ctgaagcagt atttacaatc
      841 aatatttttt tccctttaaa cttagcaaaa tttatttttc ccccttcaat gccatccact
      901 ttaaaagagt aaattttttt ttgtttgtaa attgtaaaag atgcagcagt taaaattaat
      961 acaagtaata tgaggtaatg ttttgctctc ataagctctt gattttggtg tagtaaagtt
     1021 aaaaaaaata ttgaataata taattttgtt aggaaaaatg acagtttggg gtaataccta
     1081 aataaaataa atgtatgtaa tacattgatt taaaataaga taagtaggta tttttttcgg
     1141 cttaaaaaaa attaataaac aattattgtc tttcgtaaca acctaaatct ggtaaaccaa
     1201 tacttctatt atttccatct aagtctgtta gtaaacttgt attagtgcct ttatttatag
     1261 cagcagcatt tggttttaat ctaaaattat aatatctatt atttatatta atactatcaa
     1321 aatctggtgg tatatttttt aatttattac cattaaaatt agcagtactt ggctcgttat
     1381 tttttaactt atataaagtg ttattgaagt ttacattaaa aatggtgctg ccttgttttt
     1441 taatagaaat ttcatcttct ggtattccgc catcgccata aacaatacaa ttggtaaaat
     1501 tggctgttaa atcattactg attgtttcag atactttatt actgatattt agtactggat
     1561 atttatgttg taaataataa ttaccaatac ttgtaactgt acaatgcgta aaattataat
     1621 tgccgcctgc tgttaaatat atattataac cactattact aattaaacaa ttatttgctt
     1681 caatggatga gttggtagca cccacagcaa catcataaat attatctaaa atacattcgt
     1741 ttaaaattag ttttgggttt gcattagatg atgggttttg agcaattgta ccttggtaag
     1801 catttttaat aatacaatat tgcatgacat tatttacact gttatctaaa aaatagatgc
     1861 caggccagcc accaggaaaa tctctataag gttcatctaa tctatctcct gtaaaaataa
     1921 tttttgaagg ctcgtccttt tctccaattg ctttaagcgt accttgtact aacattggag
     1981 cattggcatg aaaatatact ttggttccct ttgctatggt taatgttaca gatggttgaa
     2041 cagttaaagc accaactaca acaatgggta aatctttatt ccaagtggta tcaatagtta
     2101 ccaatttatt ttttaagaaa atagcatttt ggccataagc ttgtaactga acaaaacggg
     2161 tattaccatt atagcttatt ttaatactat cttgaataat aaatggtaaa ttggttactg
     2221 tcggatttat gtttaccata gcaaaaacat aaatactatc gttggcattt atctctatgt
     2281 tttctacaat tgatgaagag aaaccatcta cattcatttt ataaaaagaa agattacccc
     2341 ccatcaactc tacagaggtt agttttaatt ttttattgtt tggattaaaa attttaaacg
     2401 attgtgtgat ggatccaaca gaagtaaaaa cagtatcata aaataaagta tctaccgatg
     2461 ttctgagtaa tgcattgcta tctgtaataa atccttcttt tttacaactt gtaaaaataa
     2521 aagcagtaaa acatatacaa atactaaaaa gggataacaa ttttttcata taaaatctag
     2581 aaacatcgtt taaatataca atcaattaaa taaaaataaa aaggttatgg gttaatacaa
     2641 aaaagcctgc aacttttgca ggcaataatt ttgttcaatt ctttattaag cagttttagt
     2701 tgctgctttg ttggttttag ttttgtgcgg tctgctttta ccaaatgaac ctttaaaacg
     2761 tttgccttta gctgtttttt tatcacctct acccatgatt gtgtttttag ttattttaat
     2821 gaattgcagc aaaaataaaa aatcctgctg aaatgagcag gattttttat taaagttctt
     2881 aaaattagat ttttgaactt aattctttac cagctttaaa acgagctact tttttagctt
     2941 taattttaat tgtttcgcca gtttgaggat ttcttcctgt acgagctgca cgtttagaaa
     3001 cagaaaaagt accaaaacct actaaagtta ctttatctcc tttttttaat gttttagtaa
     3061 ctgtatcaac aaaagagtct aaagcagcat tagcttgagt ttttgtgata cctgcatcgt
     3121 ctgcaagttg tgcaatcaat tcagctttgt tcatgattaa ttgtgttttt aattgtttaa
     3181 ttagttagtg aaaacaaatt tattagcttt tgcaatgcaa caaaatattt ttatttgttt
     3241 ttgttttttt tatattcttt aaaaccctta ctaataaagg attttagaag aattatttaa
     3301 aatgatgcat aaaataaaac aaccttaatc ggctgaaacc atttatggca aagggtttta
     3361 gctgatattg ctcaagtgca gtaaaatcaa tagttgtaga ggaatataag aaagaaattg
     3421 aataaacaaa attgttttat aaatctatcc acaatcaatt cacaacctgc ataaatgttc
     3481 taaatgcagc aaatttatta ccccaagcca ataaaccagc ctctcttaat aaaggtgcaa
     3541 acaactcaat atacctatta taatcggctt tactctctaa ataatattga acggcatagg
     3601 ttgtattttc ttcttcctta gtgtctaaca gttttactaa ttggtatttt gtaaaacaac
     3661 ctgtattcat tacttcgggt atatgaatat tttgtagcca aattaaccat gcatcatgta
     3721 tgcttggttc tacttgaatt gttacgttat aaataaacat tatttatttt ttttagcaac
     3781 catggttaga atagttgcaa tacctgttat ttcattgctt tcatcaaaaa tttcaaccaa
     3841 ccattttaca attcctttag gtaaatcatt ttcatctttg gtatcttgtg caattttttc
     3901 tttacaggta aatcgtatgt aaatttcggc acccggataa actggtttgg taaatcttaa
     3961 ctcatcaatt ccgtagttca acaataccgg atttttttta aagccatcta caaacaaacc
     4021 tgctgcaata ctcataataa aatatccatg agctacttgt tgttcaaaca ttgttcctga
     4081 aaagtctgtt tctgttttat gggcataaaa atgatcacca ctaaggtctg caaatttgtt
     4141 gatgtcttca ctcgttatga gccttttttc tgttattaac tgatctccaa tttgtagttc
     4201 ttcaaaatat ttagtaaatg gatgacggcc atcaatattt ccttttgcat ttggttgaaa
     4261 tactttagaa actgcagtta acattgttgg gctaccttgt atggcaactc tttgcatata
     4321 atgttttatt cctcttaagc ctcccatttc ttctccacct cctgcacgtc ctggtccacc
     4381 atgtactaat aaaggtaatg gactaccatg ccctgtattt tcctttgcac attcattatt
     4441 taaaactaaa attcttccat gataagcacc tgcacctaaa acataatttg ttgcattttt
     4501 ttcagaagct gtaacaatag aacttacaag agagcctttc cccatttttg aaagctcaat
     4561 ggcttcttct aaatttttat atggcattaa tgtactcact ggcccaaaag cttctacttc
     4621 atgcacttcg ttagttaaaa atggtgtagt atttaataaa agcattgggc taataaatgc
     4681 acctttattt gcatcggcat caattgtttc tacactatct aaactaccat aaattaattg
     4741 tgaggaagcc aacagttttt gaacttgtgt ttttacttca ttacgttgtg tattacctgc
     4801 caaacttccc attctaactt tttcattcaa ggggttgcca atggtagttt gagaaagtgc
     4861 tttaattaat gcagtttgta ctgcttctat tttattttcg ggtacaaaaa ttcttcttac
     4921 agctgtacat ttttgtccac attttacggt tatttctttt ctaacttctt taataaaaat
     4981 atcccattct ggcatttcag gtgttacatc gttgcctaaa ataatgcagt ttaacgaatc
     5041 tgcttccata ttaaatggca cattttcttc taaaattctt ttatgagatt taagcattaa
     5101 gcctgtagtg gcactacctg taaaagttac tacatcttgg ctagttacat aatctaacat
     5161 attacctgca ctgccacaaa taagctgtaa agcaccgttt ggtaaaatgt tagaatccat
     5221 tatttcttta accactgctt ctgttaaata ggaagtaacg gtagctggtt ttaccacgca
     5281 tggcattcct gccaataaat taacagctat tttttctagc ataccccaaa caggaaagtt
     5341 aaaggcattg atatgtatag ctaccccttc tttaggaact agaaggtgat gacccataaa
     5401 agtattttgt ttacctaaag catgatgttc tccatctaaa caaaaaggag tatcgggtaa
     5461 ttttcttctt aaagatgcgt tagcaaatag gttaccaata ccaccttcaa tatcaatcca
     5521 actatctgct tttgtggcac ctgtttggta gctaatagaa taaaatttat ctaaatgttt
     5581 tcttaaatgg ttggctaagg ctcttaacat taaacctctt tcatgaaaac tcattttgcg
     5641 taaagcacta cctttatttc tggcataact taaaatatca tcaaagttta aacctgctgt
     5701 acttgctgtt gctattgcat ttcctgaaac tgcattatat aaaacttgcc catcaccatc
     5761 tcctgtaatt ttttttccta aaatataatt gtaaagcttt tgcataaata ttttttatga
     5821 gtaaaataca aatctaaggt aaactagttt tatgttttga atatacctgc tatgaataat
     5881 ttttatataa tttaaataag agtaggaatt aaaattcaat tatcaccatt atcaatttgt
     5941 acttattttt atagtaattt taaaagtatg aaattgcttt tttctttctt gtttttttca
     6001 ataagtattg tatctgctca acacaattat cttacattta atagagattc tactgtaa
//
LOCUS       DAOQPK010000136         6015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_7774_length_6015_cov_4.638255, whole genome shotgun sequence.
ACCESSION   DAOQPK010000136 DAOQPK010000000
VERSION     DAOQPK010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7774_length_6015_cov_4.638255"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1948
                     /locus_tag="PLU36_07220"
     CDS             <1..1948
                     /locus_tag="PLU36_07220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HMZ46577.1"
                     /translation="EITGNNLLHLAEVGAVVMNYKNIQNKKIAENALTLAEQRMGLLL
                     NNTKESFIVVNSRLRVITYNRAAQEHSPYFFTNELQSGVSILSLIKEVEVEKTIHTFE
                     KVFEGYESETETIFVDDKNIVHIYNHSFRPLYSDDEIVGVFITSTDITQRKKAEQQLK
                     ISEERYKTIIRESYDAIVITDINDIIIETSPAIEKILGYKQEELTGRIFIDLIKSDYR
                     EVAFGLLQKIKNTPDEEESVDLMATTVDGDNIWIVVKAKNLYHNKLIKGLVILIRNIT
                     KRKKVEEELSLSESRFKSLIQSGSDIISIVNKDGFVSYSSPTVKTILGNDPEKNIGKN
                     IFEYIHPDDIDWVRKQFSEMKSSGIKQLYFGPFRYRDGNDQYRWLETVVTNLLDDPAV
                     NGIVTNSRDVTERKKLTEKQNALREKLVKNNQDLQQFSFITSHNLRAPVANLLGLLNL
                     FNKENISDPFNEVLIEKFEDAANQLNNTLSDLIEILVLKSDSEIPLEEINLKNFTHQI
                     LKNIESKLTETETIITTDFKTINSIKYNKVYLESIFQNIITNAIKYRSNERKPVIHIS
                     TQEEEQWVIILFKDNGVGIDLNRYKDRVFGMYQRFHSEKEGKGLGLYIVKSQISAMGG
                     KIEVESNLNIGTTFKVYIKKNGKI"
     gene            1935..2330
                     /locus_tag="PLU36_07225"
     CDS             1935..2330
                     /locus_tag="PLU36_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ46578.1"
                     /translation="MAKFKQVLLIEDDAITIMLCERILRLNNFAETIITKQNGQEAIE
                     FIQELIQYNKPIPEICFLDINMPIMNGWDFLNEFEKIKMQLGTPMQIYILSSTANPED
                     FIKATTFKTVTGTLPKPLCKEHLIEMNFS"
     gene            complement(2327..2734)
                     /locus_tag="PLU36_07230"
     CDS             complement(2327..2734)
                     /locus_tag="PLU36_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46579.1"
                     /translation="MKKNIFLLLSLFFVSFLNAQVMSAKAKTIIIKSSNLRCWECSER
                     LDKYLTVENKSFLESAMLEWKINLLKGEIKIKYLPDRASIEDIRAVINNAGFDADDEK
                     AEPEAYKKLPTSCKREVDGGGPQKGKPCHVKPY"
     gene            complement(2758..3063)
                     /locus_tag="PLU36_07235"
     CDS             complement(2758..3063)
                     /locus_tag="PLU36_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46580.1"
                     /translation="MFKFIKKDSLPFGIILGLLGPIVGMLGFYFYRFHRLTIIEFIQY
                     LGIEQRLVTNMVSFSLLANAIIFTLYINNHIDKTAKGIFISTCFYAVFVLVLKLWFV"
     gene            complement(3073..3858)
                     /gene="surE"
                     /locus_tag="PLU36_07240"
     CDS             complement(3073..3858)
                     /gene="surE"
                     /locus_tag="PLU36_07240"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HMZ46581.1"
                     /translation="MAKITKKKEQPIILVTNDDSINAPGIKALVEAVKDLGRVVVVAP
                     DKPQSGMGHAITIGNPLRLNKVHFIDGVEAYTSNGTPVDCVKLAVDKILHRKPDICFS
                     GINHGANHSINVIYSGTMSAAMEAAIESIPSAGFSLLDFSLEADFTAAKTIVKKIAIQ
                     MLSKKLDKHLLLNVNIPAVPEKLIKGIKVCRQAYAKYKEDFVERKDPHGKLYYWLTGI
                     FVNFDKRKDTDVWALKNNYVSIVPVEFDFTDYEVKKKLESWKF"
     gene            complement(3911..4411)
                     /locus_tag="PLU36_07245"
     CDS             complement(3911..4411)
                     /locus_tag="PLU36_07245"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ46582.1"
                     /translation="MLQIRNAVENDIVAIQTIAKATWLKTYMQILSSEQMSYMLNWMY
                     SFEILKNQMQNNHAFFVGYLSQKQHEILGFAAISREAEKLFKLQKLYILPEQQKIGLG
                     KLLLQKVCNHAKENGANTLQLQVNRNNIAVNFYKKHGFKIIAEEDFDIGNGYFMNDYV
                     MELQFF"
     gene            complement(4413..4991)
                     /locus_tag="PLU36_07250"
     CDS             complement(4413..4991)
                     /locus_tag="PLU36_07250"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HMZ46583.1"
                     /translation="MLVLVCCIFNTSSLAQNLDIDILKEINPTNPNSNFWVNASASTY
                     PLATASTATLLAVSYLSKNKNLQNKGWKLAGSLLINTAITQSLKTVINRERPYEKYPT
                     LITPYEYKIGHSFPSGHTSVAFATATSLTISCKKWYVVVPAFAWASSVAFSRLYLGEH
                     YPTDIVVGALIGSGSAVISNWLTNKILINKKK"
     gene            complement(5032..5610)
                     /locus_tag="PLU36_07255"
     CDS             complement(5032..5610)
                     /locus_tag="PLU36_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46584.1"
                     /translation="MNFRILIAVVFMLSCNFANAQKMHSDSTYRKKHSVKTFTQKPAQ
                     VVFAELGGNSLIWTINYDRRFSKRLDGWGFKIGAGYFPLGGSNLFVAPVGINILTGAK
                     GHFLETGFNISFVSAKNKNKTTPKPSLFGQIDFSRNQTYLVYGVPVGYRWHKTDKPGV
                     SFRAGVEPLIGKRIDDKFVFALTGHLSIGYSF"
     gene            complement(5727..>6015)
                     /locus_tag="PLU36_07260"
     CDS             complement(5727..>6015)
                     /locus_tag="PLU36_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HMZ46585.1"
                     /translation="IKYVTNPINNTIVVAIENEKFFIETEKEINNHALKAELEKDLAY
                     QKSFLESVIKKLANEKFVQNAKPSVIEMEQKKKADCEARIKTIEESLATLL"
BASE COUNT         2150 a          934 c          833 g         2098 t
ORIGIN      
        1 agaaataacc ggcaataacc tattgcattt ggcagaggtt ggtgctgtag tgatgaatta
       61 taaaaatatt caaaataaaa aaattgctga aaatgcatta acacttgcag agcaacgaat
      121 ggggctattg ttaaacaaca ccaaagaatc ttttattgtt gttaatagca ggctaagagt
      181 aattacttat aacagagcag ctcaagaaca ttcgccttat ttttttacta atgaattgca
      241 gtctggagtt tctattttga gtttaataaa agaggtggaa gtggaaaaaa ctatccacac
      301 atttgaaaaa gtttttgaag gatatgagtc tgaaacagaa acaatttttg tagatgataa
      361 aaatatcgta catatttata atcacagttt tcgcccatta tatagcgatg atgaaatagt
      421 aggtgttttt attacctcta cagatattac acaaagaaaa aaagcagaac aacaacttaa
      481 aattagtgaa gaaaggtata aaactattat tagagaatct tatgatgcta ttgtaattac
      541 tgatattaat gatattatca tagaaacctc ccctgctatt gagaaaattt tgggctataa
      601 gcaagaagaa ttaactggta gaatttttat tgatttaatt aaaagcgatt atagagaagt
      661 tgcttttggt ttattacaaa aaataaaaaa tacgccagat gaggaagaaa gtgtggacct
      721 tatggccaca acagttgatg gagataatat ttggatagtt gttaaagcta aaaatcttta
      781 tcataataaa ttaattaaag gcttggtaat tttaataaga aatattacca aacgtaaaaa
      841 agttgaagaa gaactatcct tatctgaaag taggtttaaa agtttaattc aaagtggttc
      901 agatataata tccattgtaa ataaagatgg gtttgtatcc tactctagcc caactgttaa
      961 aacaatttta gggaacgacc ctgaaaaaaa tattgggaaa aatatttttg aatatataca
     1021 cccagatgat atagactggg tgagaaagca gttctctgaa atgaaaagca gcggtattaa
     1081 acaactatac tttggccctt ttagatatag agatggaaac gaccaatatc gttggttaga
     1141 aactgtggta accaatcttt tagacgaccc tgcagtaaat ggtattgtta ccaactcaag
     1201 agatgttact gaacgaaaaa aactaactga aaaacagaac gctttaagag aaaaactagt
     1261 aaaaaacaac caagatttac aacagttttc attcattacc tctcataatt taagagctcc
     1321 tgtagccaat ttattaggct tattaaattt atttaataaa gaaaatattt ctgacccatt
     1381 taatgaagtg ttgattgaaa aatttgaaga tgcagctaac cagttaaata atacattaag
     1441 cgatttaatt gaaatattag tattaaaatc agatagtgaa attcctcttg aagaaattaa
     1501 tttaaaaaat ttcacccatc aaatattaaa aaatatagaa agtaaactaa ccgaaacaga
     1561 aacaattata actacagatt ttaaaactat taactctata aaatacaata aggtttattt
     1621 agaaagtatt tttcaaaata ttataactaa tgcaattaaa taccgttcaa atgaacgtaa
     1681 gcccgtcatt catatttcta cacaagaaga ggaacaatgg gttataattt tgtttaaaga
     1741 taatggtgtt ggaattgatt tgaataggta taaagacagg gtatttggca tgtatcaaag
     1801 gtttcattcc gaaaaagaag gcaaaggatt agggctttat atcgtaaaat ctcaaattag
     1861 tgcaatgggt ggaaagattg aggttgagag caacttaaat ataggtacca cttttaaagt
     1921 ttatattaaa aaaaatggca aaatttaagc aagtattact aattgaagat gatgcaataa
     1981 caataatgtt atgtgaaaga attttaaggt taaacaattt tgcagaaaca ataattacaa
     2041 aacaaaacgg acaagaagca atagaattta ttcaagaact cattcaatac aataaaccta
     2101 ttccggaaat ttgtttttta gatattaata tgcctataat gaacggttgg gattttttaa
     2161 atgagtttga aaaaattaaa atgcaattag gtactccaat gcaaatttat attttatctt
     2221 ctacagcaaa tcctgaagat tttattaaag caactacctt taaaacagta acaggtactt
     2281 tacccaaacc gttgtgtaaa gagcatctta ttgaaatgaa tttttcttaa taaggtttta
     2341 catggcaggg ttttcctttt tgtggacctc ctccatccac ttctctcttg caagatgttg
     2401 gtaatttttt gtatgcctct ggttctgctt tctcgtcatc tgcatcaaaa cctgcattat
     2461 ttattacagc tcttatatct tcaatacttg ctctatcggg tagatattta atttttattt
     2521 cgcctttcag taaatttatc ttccattcta gcattgcact ttctaagaaa gatttattct
     2581 ctacagttaa atacttatct aatctttccg aacactccca acaacgcaaa ttagaagatt
     2641 taatgataat ggttttagct tttgcactca ttacctgagc atttaaaaag ctaacaaaaa
     2701 acaaactcaa cagcaagaaa atattttttt tcatactata atttttaata aatgaactta
     2761 aacaaaccat agttttaaaa ctaaaacaaa aacagcataa aaacaggttg atataaaaat
     2821 accttttgct gttttatcaa tgtggttatt aatatataat gtaaaaataa ttgcattagc
     2881 cagtaatgaa aaactaacca tattagttac caatctttgt tctataccta agtattgaat
     2941 aaattcaata attgtaagcc tgtgaaatcg ataaaaataa aaacccaaca taccaacaat
     3001 aggacctaaa agacccagga ttataccaaa tggcaaacta tcttttttta taaatttaaa
     3061 catataaaaa ttttaaaatt tccaactttc taatttcttt tttacttcat aatcggtaaa
     3121 gtcaaactca acaggtacaa tacttacata attatttttt aaagcccaaa catcagtatc
     3181 ctttctttta tcaaagttta caaaaatgcc tgtaagccaa taatacagct taccatgtgg
     3241 gtcttttctt tccacaaaat cttctttata tttagcataa gcttgcctgc aaactttaat
     3301 gccttttata agcttttctg gtactgcggg aatgtttaca tttaaaagca aatgtttatc
     3361 taatttttta gaaagcattt gtatagcaat ttttttaaca atagtttttg cagcagtaaa
     3421 atctgcttct aaactaaaat ctaataagct aaaacctgca cttggtatgc tttctattgc
     3481 agcttccata gcagcactca tagtaccact ataaataaca ttaatgctgt ggtttgcacc
     3541 atgatttatg ccactaaaac aaatatctgg tttacgatgt aaaattttat ctactgcaag
     3601 tttcacacaa tctacaggtg taccattgct ggtatatgct tctaccccat ctataaaatg
     3661 tactttgttt aatcgtaaag ggttaccaat tgttatagca tgtcccatac cactttgtgg
     3721 tttatctgga gctacaacca ctaccctgcc taaatctttt acagcttcta ccaaggcttt
     3781 tatacctggt gcatttatgc tatcatcatt agtaactaat attatgggtt gttccttctt
     3841 ctttgtaatt tttgccatga gtgcaatata ataaagagtt gattgtaata attatttttc
     3901 actatttgaa ttaaaagaat tgcaattcca taacataatc attcataaaa tagccattgc
     3961 ctatatcaaa gtcttcttca gcaataattt taaatccatg ttttttataa aagtttactg
     4021 caatattatt tctgtttact tgtaattgta atgtattagc accattttct tttgcatggt
     4081 tacacacttt ttgtagcaat agttttccta aaccaatttt ttgctgttct ggcaaaatgt
     4141 ataatttttg aagtttaaat aatttttctg cctctctaga aatagctgca aaacctagta
     4201 tttcatgttg tttttgactt aaataaccaa caaaaaaagc atggttattt tgcatttgat
     4261 ttttgaggat ttcaaaacta tacatccagt ttagcatata gctcatctgc tctgaactta
     4321 atatttgcat atatgtttta agccatgtag cttttgcaat tgtttgaatt gccacaatat
     4381 cattttctac tgcatttctt atttgcagca tattattttt ttttatttat taaaattttg
     4441 tttgttagcc aattgctgat tactgctgag ccagagccaa ttaaagcacc tactacaata
     4501 tctgttggat aatgctctcc taaatacaat cttgaaaaag caacactact agcccatgca
     4561 aatgcaggta ctacaacata ccatttttta caagaaatgg ttaaagatgt tgcagttgca
     4621 aaagctacag aagtatgtcc agatggaaat gaatggccaa ttttatattc gtaaggggta
     4681 attaatgttg ggtacttttc ataaggcctt tctctattaa taactgtttt taatgattga
     4741 gttatagccg tatttataag taaactgcca gcaagtttcc atcctttatt ttgtaaattt
     4801 ttatttttac ttaaataact tactgctaac aatgtagcag tagaagctgt tgctaatgga
     4861 taagttgaag cagaggcatt aacccaaaaa tttgaattgg gatttgttgg atttatttct
     4921 tttaagatat caatatctaa gttttgtgct aaagaagaag tattaaatat gcaacataca
     4981 agaacaagca tcaaaatttt attacaataa atttttagca tctttctatt attaaaaact
     5041 gtacccaata cttagatgtc cagttaatgc aaatacaaat ttatcatcta ttcttttacc
     5101 tatcaatggt tcaacaccag ctctaaaact tacccctggc ttatctgttt tatgccaacg
     5161 ataaccaact ggcacaccat acactaagta tgtttgattt ctggaaaaat ctatttgccc
     5221 aaacaaagaa ggctttggtg ttgttttatt tttatttttt gcactcacaa aacttatatt
     5281 aaaaccagtt tctaaaaaat gtcctttagc tcctgttaaa atatttatac ctacaggtgc
     5341 aacaaacaaa ttactgccac ctaaaggaaa atatccagca ccaattttaa aaccccaacc
     5401 atctaatctt ttactaaatc ttctgtcgta atttatcgtc caaattaaac tattaccacc
     5461 aagctcagca aacacaactt gtgcaggctt ttgtgtaaat gtttttacgc tatgcttttt
     5521 tctgtaggta gaatcggagt gcatcttttg agcatttgca aaattgcaac taagcataaa
     5581 cacaacagca ataagtattc taaaattcat ttgttaacat ttaaatattt tgtttcttaa
     5641 gaagtataat acaaatattt tacagccacc tgcagcaata ctattgaata aaattacttt
     5701 attaaaccca tttgaactaa cacatattat aataaagttg ctaaactttc ttctattgtt
     5761 ttaattcttg cttcacaatc ggctttcttc ttttgctcca tttcaattac agaaggtttt
     5821 gcattttgca caaatttctc gttagctaat ttcttgataa cgctttctaa aaaacttttt
     5881 tgatatgcta aatctttttc tagttcagct tttaatgcat gattattaat ttctttttcg
     5941 gtctcaataa aaaacttttc gttttcaata gcaactacaa tagtattatt tattgggttg
     6001 gtaacatact taata
//
LOCUS       DAOQPK010000137         5963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_7870_length_5963_cov_3.993907, whole genome shotgun sequence.
ACCESSION   DAOQPK010000137 DAOQPK010000000
VERSION     DAOQPK010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7870_length_5963_cov_3.993907"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PLU36_07265"
     CDS             complement(<1..469)
                     /locus_tag="PLU36_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46586.1"
                     /translation="MKVHFLSISLFSSIPSLRFIVDSFLDKNFRVAITELSIKNSNNY
                     FVNKNIEHNLIAEFDNFDRLKVGLKKIAFKKYLTICKILLSKKDDLIYTNDFQVVFLK
                     LLLNKKSKLIYHQFELIEPTFLGKIGNFVFKYVLKNANKIDLCIFPEQNRADYF"
     gene            complement(473..1501)
                     /locus_tag="PLU36_07270"
     CDS             complement(473..1501)
                     /locus_tag="PLU36_07270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ46587.1"
                     /translation="MNIAIIGKFYTEGFGLHIEETLSDMGHTIVRIDPEMEFLQYNFL
                     GKRIKNINKTLYQQVFHKIPAIRNFKSKDIYNSYKANKIDFTIVTHDFLTKQEIDTIK
                     KINPSPIVLWFPDAISNFQKSMFFVAGYDYLFFVDKYIVEVLKSEFNFNIHYLPQCCN
                     PKKHNRVVLNEQDINFYSCDITNAGNLYPSRSALYKHLLNYNIKMWGAPPSIWLKLPE
                     LDKIIMNKSVHNQEKSKAFMAAKIVLNNLHPAVINGVNKRTFEIPACGGLQFTSYRAA
                     LNELFEVGKEVVAYKDLEDLKNKIDYFINPQNEAERLQIIEAGYKRAITEHTYEQRLN
                     QLLDTVFN"
     gene            complement(1517..2551)
                     /locus_tag="PLU36_07275"
     CDS             complement(1517..2551)
                     /locus_tag="PLU36_07275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ46588.1"
                     /translation="MKISICVVQYNRIELLLKSLSIIEKQTYHDIEICISDDCSTDNT
                     VDEILKLKQTYKYPIVFHRNEVNKGFDFNYRKSIEIASGDYAFVIGNDDSINGENSIA
                     ELVKFLEENNYPDLGFCNMLEERTGNTVIKRAMNTTILGSGAGVAIKYYSCFVFVGGL
                     IYKRNTFLKYNTDQYDGSVYTQMYLSVFMICKGERAFSIKEPMVLKDILLDGKFRHSY
                     RDRIAKTWDKYFETNAGLFGLMHVVISGLKNAGLLQQKHIYKIFKRVYLMTYPQWILD
                     YKENNAFPSAVGLFVGINPLKADDFKLLNFFNKIRIFCLYVFASIVAFLTPVFLFKKF
                     KQKLYEYFKK"
     gene            complement(2511..2774)
                     /locus_tag="PLU36_07280"
     CDS             complement(2511..2774)
                     /locus_tag="PLU36_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46589.1"
                     /translation="MKNLAIIITHPIQYYAPVFQLLAKCCTIKLFYTFGEKVLKDKYD
                     PGFGKIITWETSLLISTHESYLSVYLEYKNNENINLCSSVQSY"
     gene            complement(2804..3223)
                     /locus_tag="PLU36_07285"
     CDS             complement(2804..3223)
                     /locus_tag="PLU36_07285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HMZ46590.1"
                     /translation="MPNKSIKVIFDTNIWISFLIGKHLAKIKRYLSNGSIIIITSEQL
                     ISEIKVVTGRAKLKKYFPHDSVEEMIELLETIAIKVETKSTHFVSRDPKDNFLLDLID
                     FSKADFLVTGDKDLLELSPFKTAQILTPTEFETQLKN"
     gene            complement(3204..3437)
                     /locus_tag="PLU36_07290"
     CDS             complement(3204..3437)
                     /locus_tag="PLU36_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46591.1"
                     /translation="MMKTALQIDLTFDQILSVIKQLPKQQKIMLSKELEKEVIDSKLS
                     RLLKTFHTKELDLKTITKEVEIVRQDAYAKQKH"
     gene            complement(3630..4286)
                     /locus_tag="PLU36_07295"
     CDS             complement(3630..4286)
                     /locus_tag="PLU36_07295"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMZ46592.1"
                     /translation="MIKLNLGCGSVRPEGWINTDSSLNANIQRIPFIGKLVTKTFGKV
                     QYSSSNFIYMNLNKKWQYADNSVDIVYASHLFEHLTQKAADLFLKEAYRTLKVGGTLR
                     IVVPDLYQIATKYLAEYDTKNDNEDTTKFIMWAINMHREGQYGNPSILRKIIYEWQGY
                     PHQHKFMYDPKSLSLKVKNYGFNNIHLMSYGKSEYIKEILDVEGTSESYLSVYLECKK
                     "
     gene            complement(4318..5283)
                     /locus_tag="PLU36_07300"
     CDS             complement(4318..5283)
                     /locus_tag="PLU36_07300"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HMZ46593.1"
                     /translation="MKQFALIGAAGFIAPRHMKAIKDTGNNIVCALDNFDSVGILDSY
                     FPNADFFTEFERFDRHIEKLRLNNTPIDYVSVCSPNYLHDAHIRFGLRNNTDVICEKP
                     IVLNPWNIDALIDMEKQTGKKIFNILQLRLHPAIIELKEKVANADANKVYDIDLTYIT
                     SRGNWYYTSWKGNIEKSGGIATNIGVHFYDMLQWIFGEVKQNIVHIHEHDRAAGYLEF
                     KQARVRWFLSIDYNTIPQALRNEGKRTYRSLTINDESFEFSDGFTELHTNSYKHILSG
                     AGFRIEEARTAINIVHHIRTQKPIGLQGNYHPFAKLPLSKHPFSK"
     gene            complement(5327..>5963)
                     /locus_tag="PLU36_07305"
     CDS             complement(5327..>5963)
                     /locus_tag="PLU36_07305"
                     /inference="COORDINATES: protein motif:HMM:NF018009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HMZ46594.1"
                     /translation="TIENKWSSTVLLHQIESGLYQRQYKTNKTTNFHLTLSKTQSDLA
                     LEIFKDPYKFDFLSLTERITEKELENNLVNQITKFLLELGNGFAFIGNQYKVKTPRKA
                     YAIDMLFYHLKLKAYIVIELKMEAFSPGDASQLNFYLNLVDDFVKDKNDKPSIGILMC
                     KAKDNFEVEYALRGLNKPIGVAEYKMLPKKIREQLPSSTQFRNMLKKIKNL"
BASE COUNT         1972 a          945 c          743 g         2303 t
ORIGIN      
        1 taaaataatc agctctattt tgttcaggaa aaatacatag atctattttg tttgcatttt
       61 tcaacacata tttaaaaaca aaatttccaa tttttccaag aaaggttggc tcaattaatt
      121 caaactgatg gtatattagt tttgattttt tgttcaataa tagttttaga aaaacaacct
      181 gaaaatcgtt tgtataaatt agatcatctt ttttgcttag caatatttta catatagtaa
      241 ggtatttttt aaaagctatt ttctttaacc caacttttaa gcgatcaaaa ttgtcaaact
      301 cagctattaa attatgttct atattcttat ttacaaaata gttatttgaa tttttaatag
      361 ataattcggt aatggcaact ctgaaatttt tatctaaaaa agaatctaca ataaatctta
      421 aagatggtat ggaactaaat aagcttattg ataaaaagtg aactttcatg tattagttaa
      481 aaactgtatc cagtaattga tttaatctct gttcataagt gtgttctgta atagctcttt
      541 tataacctgc ttcaataatt tgcagtcttt ctgcttcatt ttgtggattt ataaaataat
      601 ctattttatt ttttaaatct tctaaatctt tataagccac tacttccttc cctacttcaa
      661 acaattcatt tagtgcagcc ctataactag taaactgcaa gccaccacaa gctggtattt
      721 caaaagttct tttatttacc ccatttatta cagcaggatg taaattattt aatacaattt
      781 tagctgccat aaaagctttt gatttttctt ggttatgaac agatttgttc atgataattt
      841 tatctaattc tggcagtttt aaccaaattg aaggtggtgc cccccacatt ttaatgttat
      901 aattgagaag gtgtttatat aatgcagatc tactaggata taagttccct gcattggtta
      961 tatcacaact ataaaagtta atatcttgtt cattcaatac aactctattg tgttttttag
     1021 ggttacagca ttgaggtaaa taatgaatat taaaattaaa ttcagatttt aatacttcaa
     1081 caatatattt atctacaaaa aataaatagt cgtaacctgc tacaaaaaac attgattttt
     1141 gaaaattact tatagcatca ggaaaccata aaacaattgg agatggatta atttttttga
     1201 ttgtatctat ttcttgcttg gttaaaaagt catgtgttac aatagtaaaa tcaattttat
     1261 ttgctttata actattataa atatctttag acttaaaatt tctaatagct ggtattttat
     1321 gaaaaacttg ctgataaagc gttttattaa tgtttttaat tcgtttacct aaaaaattgt
     1381 attgtaaaaa ttccatttca ggatctattc ttacaatagt atgccccata tctgacaaag
     1441 tttcttctat atgcaaacca aacccttctg tataaaactt gccaataatt gcaatgttca
     1501 taattactac tttaacctac tttttaaaat actcgtataa tttttgctta aattttttaa
     1561 acaaaaaaac tggtgttaaa aatgcaacaa tagatgcaaa aacgtataaa caaaaaattc
     1621 ttattttatt aaaaaaatta agcaatttaa aatcatctgc tttaagagga ttgataccta
     1681 caaacaaacc aacagcactc ggaaaagcat tgttttcttt gtaatccaat atccattgag
     1741 gataagtcat taaataaacc cttttaaata ttttatagat gtgtttttgc tgtaaaaggc
     1801 cagcattttt taacccactt ataactacat gcattaatcc aaataaccct gcatttgttt
     1861 caaaatattt atcccatgtt ttagcaattc tatctctgta agaatgtctg aatttgccat
     1921 ctaacaaaat atcttttaat accatgggtt ctttaatact aaatgctctt tcacccttgc
     1981 atatcataaa aacgcttaaa tacatttgtg tataaacact tccatcatat tggtctgtat
     2041 tgtattttaa gaaagtgttt cttttataaa ttagccctcc tacaaaaaca aagcaactgt
     2101 aatattttat tgcaacacca gcacctgaac ctaaaattgt tgtattcatt gctcttttaa
     2161 taactgtatt gcctgttctt tcttccagca tattacaaaa gcctaaatct ggataattat
     2221 tctcttctaa aaactttacc aattctgcaa tgctgttttc tccatttatg gaatcatcat
     2281 taccaataac aaaagcataa tcacccgatg ctatttcaat actttttcta taattaaaat
     2341 caaatccttt gttcacttca tttctatgaa atacaatagg atatttataa gtttgtttta
     2401 gttttaggat ttcatcaaca gtattatcgg tagagcaatc atcgctgata caaatttcta
     2461 tatcatgata agtttgcttt tctataattg acaaactttt taacaataat tcaatacgat
     2521 tgtactgaac tacacaaatt gatattttca ttatttttat attctaaata aacagataaa
     2581 tacgactcat gagtgcttat caataaagaa gtttcccatg taattatttt tccaaaacca
     2641 gggtcatatt tatctttcaa aaccttttcg ccaaaagtat aaaacaattt tatagtacaa
     2701 cacttagcta ataactgaaa tactggtgca taatactgaa taggatgtgt tatgatgatg
     2761 gcaaggtttt tcatgtgtat atttcaaaca aattttctat acactaattt ttaagttgag
     2821 tttcaaattc agtaggtgtc aggatttgag cagttttaaa agggctgagt tctaataaat
     2881 ctttatcgcc tgttactaaa aagtctgctt ttgaaaaatc aatcaagtct aaaagaaaat
     2941 tatcttttgg gtctctactt acaaaatgtg ttgattttgt ttccactttt atagcaatag
     3001 tttccaagag ttcaatcatt tcttcaacac tatcatgagg gaagtatttc tttaattttg
     3061 ctctcccagt tactacttta atttctgaaa ttaattgctc tgaagttata ataatgatac
     3121 ttccgttaga gaggtatcgt ttaattttag ctaaatgttt tccaattaga aagctaatcc
     3181 aaatattagt atcaaaaatt actttaatgc ttttgtttgg cataggcgtc ttgtcttaca
     3241 atttcaactt cctttgttat tgttttcaaa tcaagttctt ttgtatgaaa tgtttttaac
     3301 aaacgagata gtttgctatc aataacttct ttttctaact cttttgataa cattattttt
     3361 tgctgtttag gtaattgttt tattacagat aatatttgat caaaagttag gtctatttgc
     3421 aatgccgttt tcatcattta tttaattaaa tattttatca aacttactac aatatttgct
     3481 gtcttccaat aaaggtaaat cccatttaat caccttacca aaaccagggt catatttatc
     3541 tttcaatacc tcttagcacc aagtataaaa cactggtgca tagtactgaa tagggtgtgt
     3601 aatgatgatg gcgaggtgca agaaattaat cattttttac attctaaata aacagataaa
     3661 tacgactcac ttgtaccttc tacatctagt atttctttaa tgtactcact tttaccataa
     3721 ctcattaagt gaatattatt aaacccataa ttttttactt ttaaacttaa acttttgggg
     3781 tcgtacataa atttatgctg gtgagggtat ccctgccatt cataaataat ttttcttaaa
     3841 atacttgggt tgccatattg cccctctctg tgcatattaa ttgcccacat tataaatttg
     3901 gtagtatctt cattatcatt ttttgtatcg tactctgcta aatatttagt tgcaatttgg
     3961 tataaatcgg gtacaacaat tctcagagtt ccaccaacct ttaaagttct gtatgcttct
     4021 tttaaaaata agtctgcagc cttttgggta aggtgctcaa ataaatgtga ggcatatact
     4081 atatcaacgg aattatcagc atattgccat tttttattta agttcatata aataaaatta
     4141 gaactgctgt attgtacttt accaaatgtt tttgtaacca attttccaat aaatggaatt
     4201 ctttgaatat ttgcattaag agaagaatct gtgttaatcc aaccctctgg tctaactgaa
     4261 ccacagccta aatttaattt tatcattttt tgaatttaaa taatttctta accaatttta
     4321 tttactaaaa ggatgtttac ttaaaggtaa ttttgcaaaa ggatgataat taccttgtaa
     4381 acctattggt ttttgtgtac gaatatggtg cacaatattt atagctgttc gggcttcctc
     4441 aattctaaaa cctgcacctg ataaaatgtg tttataactg ttggtgtgca attctgtaaa
     4501 gccatcacta aactcaaacg actcatcgtt tattgttaaa gaacgatagg ttctcttacc
     4561 ttcatttctt aatgcttgtg gtatggtatt ataatctata cttaaaaacc aacgtactct
     4621 tgcttgttta aactctaaat aacctgctgc tctatcatgc tcatgtatat gaacaatatt
     4681 ttgttttact tcgccaaaaa tccattgaag catatcgtaa aaatgcactc caatattagt
     4741 agcaatacca ccagattttt ctatatttcc tttccaactg gtataatacc aattgcctct
     4801 ggaagtaatg taagttaaat ctatatcgta cactttatta gcatctgcat tagctacttt
     4861 ttcttttaat tctataatgg ctggatgtaa tctgagttgt aaaatattaa atattttttt
     4921 gcctgtttgc ttttccatat ctattaaagc atcaatattc caagggttta atacaatagg
     4981 tttttcgcaa ataacatctg tattatttct taaaccaaaa cgtatatgag catcatgcaa
     5041 ataattgggt gagcaaacag aaacataatc tattggagta ttgtttaaac gtaatttttc
     5101 tatatgcctg tcaaaacgtt caaactctgt aaaaaaatca gcattgggaa aataactatc
     5161 caaaatacct acgctatcaa aattatctaa agcacacaca atattgttgc ctgtatcttt
     5221 aatagctttc atgtgtcgtg gtgcaataaa acctgcagca ccaataagag caaattgttt
     5281 catgtattat attttattta cctcctcact tttttagttg atattgttat aaatttttaa
     5341 tttttttaag catattacga aattgagtag agcttggtaa ttgctctctt atttttttag
     5401 gaagcatttt atactctgct acaccaattg gtttgtttaa gcctcttaat gcatactcca
     5461 cttcaaaatt atcttttgcc ttacacatta atattccaat agaaggctta tcgttcttgt
     5521 cttttacaaa atcatcaaca aggtttaaat aaaaatttag ttggcttgca tcgcctggcg
     5581 aaaacgcttc catttttaat tctataacaa tatatgcttt tagttttaag tgataaaaca
     5641 acatatctat tgcataagct ttacgaggtg tttttacttt atactgatta cctataaatg
     5701 caaaaccatt acctaattcc aacaaaaatt tggttatttg attaactaaa ttattttcta
     5761 attccttctc tgtaattctt tcagttaaag aaagaaaatc gaatttgtaa gggtctttga
     5821 aaatctctaa agctaagtcg ctttgcgttt tagataacgt aagatgaaag ttggttgttt
     5881 tgtttgtttt atactgcctt tggtataagc ctgattctat ttgatgcagc aatactgtac
     5941 tactccattt attttctatt gtt
//
LOCUS       DAOQPK010000138         5924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_7956_length_5924_cov_3.523769, whole genome shotgun sequence.
ACCESSION   DAOQPK010000138 DAOQPK010000000
VERSION     DAOQPK010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7956_length_5924_cov_3.523769"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PLU36_07310"
     CDS             <1..231
                     /locus_tag="PLU36_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018623218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMZ46595.1"
                     /translation="GLCYIACEDTSHQAINLQHQNPYNIYTIKEDDCVGCNLCKLVCP
                     VDNCITMDEKRVAPEYINWKMWQEKGLPLNDH"
     gene            329..1564
                     /locus_tag="PLU36_07315"
     CDS             329..1564
                     /locus_tag="PLU36_07315"
                     /inference="COORDINATES: protein motif:HMM:NF006771.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HMZ46596.1"
                     /translation="MNINTTRLQQHFEAMSLIGKIGESGTNRPAHSADEKKAFALAAS
                     WMQEAGMTTKIDNFGNLIGTLQGKNPNLPILMMGSHLDSQPYGGRFDGVAGVLCAIEV
                     VTTLTENKIKPQRTIQVISFADEEGWRFNKGLFGSRGILGKLEEGELQRTDANGITRE
                     QALLDFGCDITKFKNDEYAPNSIYCFLELHIEQGPVLEIANKPVGVVSGISGPLWATV
                     KLKGMAGHAGSVPMAIRKDALVGAAEIIVALNQIASQVPNAPTVGTVGTLNVFPASRN
                     IIPEEVSFTIDLRDIDLERRHGYEKQLMDAIENISKKHGLTYQISEDTNSEPRYCANW
                     IKTIIHNECKKLNVDAPELMSGPFHDSLPLSYACDYGMIFIRSKDGISHNPLEYSSYE
                     DIALGTQVLLGTVQQILDI"
     gene            complement(1769..2146)
                     /locus_tag="PLU36_07320"
     CDS             complement(1769..2146)
                     /locus_tag="PLU36_07320"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ46597.1"
                     /translation="MVGNKIKIIREIKNYTQEYMASELNISQNAYSRIELNKTKLTTA
                     LAEKIADILEVSLADLLSKDNPIITFSNNTIDKNFGYINNHYESQKELYENTITLLQK
                     ELTDAKEREERLLKLLEKRGYKS"
     gene            2292..3809
                     /locus_tag="PLU36_07325"
     CDS             2292..3809
                     /locus_tag="PLU36_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46598.1"
                     /translation="MMKSIFSFVLFILISPTTYSQSIDTTLLSNYPSSVAIKVHEVGS
                     KIMLTNNEQFMLAELFLEEEQTIGNAILVGSSTAIIDSLKRLFKNDFNLLLPASKIDN
                     YYNAIALPKANTIATLTAAMLQQKYNTDTLTQQYFNTIYGWRENLIEKVWAKSTDTLI
                     RNNILQNIITVYDSVISRYTLSAASSHYLAIRLYYIDSVLSIPANQKNALVESYFMNC
                     MQHKNRAYSDNFNTAFNAVFTQPQDTVYYAAAYNNEITRNSIEAAQVALSVYIKTYSL
                     SAMAATQIIPYLVQRERAIALSNKLYANYSEAKDSLINNILLIHQPIIDSVVSLYAHL
                     YNNSQIDIAIKFATEIDLDENQLNTLHQAVATLKEMQTTFRETDPFGEFDSKAYESEV
                     LNSVLTPEQYTYVLEAKYYSKATAMANKDWTELVRLNIAGELSLQEAITKTELTNYHV
                     AIFIAYYRNANNPEEQYISIQRINEVMPETMRLLFDRWAESGTPYGNLPDVFFQW"
     gene            3964..5376
                     /locus_tag="PLU36_07330"
     CDS             3964..5376
                     /locus_tag="PLU36_07330"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ46599.1"
                     /translation="MKKILFILLLFSCYTAAYAQSDSTAITDSAELYLTGTFKKYNPQ
                     KAFQLFTLRANAGEPKAMNALGMHYAKGLGVDSNFALAKYWFLQAANNGYHKAWVNAG
                     MLYKTHSTDSTGYAAAVQYFISAVQLQEPSAFFALGYMYYKGLGCTQSYTQALQYFKT
                     GNTTGSAACMYFTGLCFKYGYGINANTDSATYYITQAAAKGYKQAQAELTAYNNYAAR
                     SLQKTQQINIVALQEPDMPYTKTKSTTIYTNIDGSYTGTLTQYDYSGKKILAQIPLTL
                     QLFSNGKMITGSWQQINEPAITLNAVQQGNELLFTANNFITTTILARRKQQKIVFKSA
                     AFTQHATTDSIYLQGSLLLFNTYTKETDKPVQITLAKKKTINNNPTTDNLIVFPNPVK
                     ENNSINIRFTLHKATPVRISIHNALGQVVYTHIHTEVLKAGIHEIRLSGVQTALKTAG
                     IYILQVKGNGIQQTTAFIKE"
     gene            5390..>5924
                     /locus_tag="PLU36_07335"
     CDS             5390..>5924
                     /locus_tag="PLU36_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46600.1"
                     /translation="MKKILILLVLLYTAITVMAQSNNDSISFTHITTTDFMEAISGGK
                     ISWSTMSRPKPIKCPKVDGNCGGFWNGIGNFFAGLGGAFWSGVKYVLASMFDGEGGGA
                     GGGGFVSINLFNFPGAYGPPSFVPPGGSGGGGSATTNTTNPLGNTTIPPPVKPAVDDE
                     IPDSTASLKKDCAGVKGG"
BASE COUNT         2160 a          972 c         1004 g         1788 t
ORIGIN      
        1 ggtttgtgtt acattgcttg tgaagatact tcgcatcaag ccataaattt acaacaccaa
       61 aatccttaca acatatacac tataaaagaa gatgattgcg taggctgcaa tttatgtaag
      121 ttggtttgcc ctgtagataa ttgtattaca atggatgaaa aacgtgtagc tccagaatat
      181 attaactgga aaatgtggca agagaaaggc ttaccattaa acgaccatta aaattatttt
      241 gaatgatgaa ttttaaatga ttttcaaagt cattcaactt agttctattt aacttcttca
      301 attcaaaatt tataatttaa aatttataat gaatatcaat acaacccgtt tacagcaaca
      361 ctttgaggct atgagcctta taggaaaaat tggagaatca ggtaccaatc gtcctgcaca
      421 ttctgcagat gaaaaaaaag catttgcatt agcagcctct tggatgcaag aagcaggcat
      481 gactacaaag attgataatt ttggaaattt gataggaacc ctacaaggca aaaatcccaa
      541 tctgcctatt ttaatgatgg gatctcattt agattcacag ccctatggag ggcgttttga
      601 tggtgttgca ggcgtattat gtgctattga agttgttaca acacttactg aaaataaaat
      661 taaaccgcaa cgcactattc aagtaatttc ttttgcagac gaagaaggat ggcgttttaa
      721 caaaggctta tttggtagca gaggaatttt aggaaaatta gaagaaggag agctgcaacg
      781 tacagatgca aacggcatta caagagaaca agcgttatta gattttggtt gcgacataac
      841 caaatttaaa aacgatgaat atgcacccaa tagcatttac tgttttttag aattgcatat
      901 agagcaagga ccagtattag aaattgccaa taaacctgtt ggtgtagttt caggtatttc
      961 aggaccttta tgggctacag taaaattaaa aggcatggct ggtcatgcag gaagcgtacc
     1021 tatggctata agaaaagatg ctttagtagg tgctgctgaa attattgttg cattaaatca
     1081 aattgcttcc caagtgccta atgcaccaac tgtaggtaca gtaggtactt taaatgtttt
     1141 tcctgcaagt agaaatatta ttccagaaga agtaagtttt actattgatt taagagatat
     1201 tgatttagaa agaagacatg gttatgaaaa acaattaatg gatgctatag aaaatatttc
     1261 aaaaaaacat ggattaactt atcaaatttc tgaagacact aatagtgaac ctcgttattg
     1321 tgcaaactgg ataaaaacta ttatacacaa tgaatgtaaa aaactaaatg tagatgcacc
     1381 tgaattaatg agtggtcctt ttcatgattc attaccactt tcttatgctt gtgattatgg
     1441 aatgattttt ataagaagta aagatggtat aagccataac cctttggaat attcatctta
     1501 cgaagatatt gctttgggaa cacaagtttt acttggaact gtacagcaaa tactggatat
     1561 ataagaatca tcttttacaa aacatttttt ctgtcattct gaacgaagtg aagaaaaaaa
     1621 ctatgtcatg ttgaacgaag tgaaacaaaa aaacgctgtc attctgaacg aagtgaagaa
     1681 tctgcccaaa caatagtact gcatttcagc agatccttcg ctttgctcag gatgacaaaa
     1741 atctaaaacg atatattatt tgaacatatt agcttttata tccccttttt tctaataatt
     1801 tcaacagtct ttcttctctt tcttttgcat ctgttaattc tttttgtaat aaggttattg
     1861 tgttttcgta cagttctttt tgcgattcgt agtggttatt aatatagcca aaatttttgt
     1921 cgatagtatt attgctaaaa gttataattg ggttatcttt tgataataaa tcagcaagag
     1981 aaacttccaa aatatctgca attttttctg ctaaagcagt agtcagtttt gttttattca
     2041 actcaatacg tgagtaagca ttttggctaa tgtttaattc agaagccata tactcttgag
     2101 tgtagttttt aatttctctg ataattttta ttttattacc caccatagct atttgtttta
     2161 aattacttaa taagtggcta aattactcat ttaggttgtt gtttaatcaa atatataaaa
     2221 gctcctttgt atagaattta aaaaggatac ttttttattt tctacaatta tataaaagtc
     2281 aaaaataatt tatgatgaag tcaatatttt catttgtatt atttatactg atttctccaa
     2341 ctacctacag ccaaagcata gatacaacat tattatccaa ttatccttct tctgtagcta
     2401 tcaaagtgca tgaggtaggt tcaaaaataa tgcttacaaa taatgagcaa ttcatgcttg
     2461 ccgagttatt cttagaggaa gaacaaacaa taggtaatgc cattttagtt ggttcatcta
     2521 ctgcaataat tgacagctta aaaagacttt tcaagaatga ctttaatttg ttattacctg
     2581 cttcaaaaat agataactat tataatgcaa tagcattacc caaagctaat accattgcta
     2641 cacttacagc agctatgctg caacaaaaat ataataccga tacgcttaca cagcaatatt
     2701 ttaataccat ttatggatgg cgtgaaaacc tgatagaaaa agtatgggca aaaagtacag
     2761 acactttaat tcgtaataat atcctgcaaa atattattac tgtttatgat agtgtaataa
     2821 gcagatatac actttctgca gcaagtagtc attatttggc aatcagatta tattatatag
     2881 atagtgtact aagcattcct gctaatcaaa aaaatgcttt agtagaatct tattttatga
     2941 attgcatgca gcataaaaac agagcctact ccgataattt taatacagca tttaatgcgg
     3001 tatttacaca accgcaagac accgtttatt atgctgctgc ttataacaac gaaattacaa
     3061 gaaacagtat agaagctgca caggttgcat tatcggttta cattaaaact tacagtttat
     3121 ctgcaatggc ggcaacacaa ataattcctt acttagtaca aagagaaaga gcaatagctt
     3181 taagtaacaa actgtatgct aattattcag aagccaaaga cagtttgata aataatattt
     3241 tactcatcca tcaacctata atagatagtg tagtaagctt atatgcccat ttatataata
     3301 actctcaaat agatattgcc ataaaatttg ctactgaaat tgatttagat gaaaaccagc
     3361 ttaatacact acatcaggca gttgcaactt taaaagaaat gcaaacaacg tttagagaaa
     3421 ccgacccttt tggagagttt gacagcaaag cctacgaaag tgaagtatta aactctgtgc
     3481 ttacaccaga acaatatact tatgtattag aagcaaagta ttatagcaaa gctactgcta
     3541 tggcaaataa agactggaca gaattggtaa gattaaatat agccggcgaa ttaagtttac
     3601 aggaagctat tactaaaaca gaactaacca attatcatgt agccatattt atagcttatt
     3661 accgaaatgc caataaccca gaagagcaat acatcagtat tcaaagaata aatgaagtga
     3721 tgcccgaaac aatgagatta ttatttgacc gttgggcaga atccggtaca ccttatggca
     3781 atttacctga tgtttttttt caatggtagt tttttaaaat ttaaaattaa gaattaaaaa
     3841 ttaaaaatta taaattaaga actagtagaa agcgtaatga tgaatgttga atgtttaatg
     3901 atggaaactg gtgtatctaa ttcctaatta cccataaaac gaataaacct attaactgat
     3961 aaaatgaaaa aaatattgtt tatcttattg ttgttttctt gctacacagc agcctatgca
     4021 cagtctgata gtacagccat tacagacagt gcagagttgt atcttactgg cacatttaaa
     4081 aagtataatc ctcaaaaagc atttcagtta tttaccctac gagctaatgc aggcgagcca
     4141 aaagccatga atgccttagg aatgcactac gcaaaaggct tgggcgttga tagcaatttt
     4201 gctttagcaa aatactggtt tttacaggca gccaataatg gataccataa agcttgggtt
     4261 aatgcaggta tgctgtataa aacacacagt acagatagta caggttatgc agcagcagtg
     4321 caatatttta tttcagcagt acaattacag gaaccttctg cattttttgc attaggctat
     4381 atgtattaca aaggtttggg ctgtacacaa agctatacac aggctttaca atattttaaa
     4441 acaggcaata ccacaggcag tgcagcatgt atgtatttta caggtttatg ttttaaatac
     4501 ggttatggta taaatgccaa tacagacagt gccacttact atataacaca agctgctgct
     4561 aagggctaca aacaagcaca ggcagaactt acagcctata ataattatgc tgcaagaagc
     4621 ctgcaaaaaa cacaacagat aaacatagta gcgttgcaag aacctgatat gccctataca
     4681 aaaacaaaat ctactacaat atatacaaac atagatggta gctatacagg aacacttaca
     4741 caatacgatt acagcggaaa aaaaatactt gcacaaatac cattgacttt acaattattc
     4801 agcaatggaa aaatgataac aggcagttgg cagcaaatca atgaacctgc aataacctta
     4861 aacgcagtac aacaaggcaa cgaattacta tttactgcca acaactttat tacaacaacc
     4921 atactggcaa gaagaaagca acaaaaaata gtgtttaaat ctgctgcttt tacacaacat
     4981 gccacaacag acagtattta tttacaagga agtttgcttt tatttaatac ctataccaaa
     5041 gaaacagaca agcctgtaca aataaccctt gccaagaaaa aaacaataaa caataaccct
     5101 acaaccgata acctgattgt ttttcccaac cctgtaaaag aaaataatag cataaatata
     5161 aggtttacac tacacaaagc cacacctgta agaataagca tacacaatgc acttgggcaa
     5221 gtagtatata cacatataca tactgaagtt ttaaaagcag gcatacatga aatacgatta
     5281 agcggtgtac aaacagcatt aaaaacagca ggtatttata tactgcaagt aaaaggaaac
     5341 ggcatacagc aaacaacagc atttataaaa gaataaacag gtaatactaa tgaaaaaaat
     5401 tttaatttta ctggtactac tttatacagc aattactgta atggcacaaa gcaataacga
     5461 tagcattagt tttacccata ttaccaccac cgattttatg gaggcaatta gtggtggaaa
     5521 aattagttgg tcaacaatga gcagaccaaa accaattaaa tgtccaaaag tagatggtaa
     5581 ttgcggtggt ttttggaatg gaataggtaa tttttttgct gggctggggg gtgctttttg
     5641 gagtggggta aaatatgtac tagcctcaat gtttgatggt gaaggtggag gggcaggtgg
     5701 tggaggtttt gtatcaataa accttttcaa ttttccagga gcttatggac caccaagctt
     5761 tgtaccacct ggtggcagtg gaggaggtgg cagtgctaca accaatacca ccaacccatt
     5821 aggtaatacc actataccac cacctgtaaa acctgcggta gatgatgaaa tacccgacag
     5881 cactgcctct cttaaaaaag attgtgcagg ggtaaaaggt ggta
//
LOCUS       DAOQPK010000139         5921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_7964_length_5921_cov_3.965394, whole genome shotgun sequence.
ACCESSION   DAOQPK010000139 DAOQPK010000000
VERSION     DAOQPK010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5921
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7964_length_5921_cov_3.965394"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="PLU36_07340"
     CDS             <1..1136
                     /locus_tag="PLU36_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46601.1"
                     /translation="TKRPNVPVHMDMPGWANTAGFYSKLEGKMNNHNFLINLNAHYNT
                     SLAEMTMYSKNSNEKDMFMLTWPGVHTIFGKLFVEDKIDLNKNWSANISAGLSINNNT
                     IKNELGLTSLQIFYPNLLASKTRLLKNIAGKIYYRKNQWQQSFGVGFGERAPSISEGY
                     GFYLFNSFDRYDYIGNPTLKNEKSLEINSSTSYNTKILSIHFQADYFRIFNYIIGMPN
                     HNFIPMTIGANGVKVYQQLPYVNIFNLSFSSTYRITNSIQIAGKMVYRKGNYESLNLP
                     QIQPISYGSTIRYQHKNFIAECTVEGALKHTNFSPTFGETGTDDYTILNLSASKQIKI
                     FNQTVQIKTGFENLFDRYYSTFADWNKIPRNGRNFFINVVYNF"
     gene            1206..2186
                     /locus_tag="PLU36_07345"
     CDS             1206..2186
                     /locus_tag="PLU36_07345"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HMZ46602.1"
                     /translation="MLCIYHQSTDPYFNIATDEYILKHIEEDCFMLWRNDNAIIVGKH
                     QNALAEINYEYVKQNNIHVVRRLSGGGAVYHDLGNLNFSFTKQGKTSEMVDFKLYTQP
                     IIEVLHSLGIEAKFEGRNDITIDGMKISGNAEHLFKHKLLHHGTLLFSSQMKNVSEAL
                     KIHPLKYQDKAVKSVPKRVTNISQHLKIPITLEEFTQKIMNHIVSTTPNATLYEFTKD
                     DFKKIEAIKQERYVKEDWNFGLIANYNFNKSIKTNGGIVEIHLIVEKGIIKEAKIYGD
                     FFNLKDIAEIENALQNSRHDENSIRKIFENFVIDEYFMNITLDEIISAMF"
     gene            2286..3476
                     /gene="kbl"
                     /locus_tag="PLU36_07350"
     CDS             2286..3476
                     /gene="kbl"
                     /locus_tag="PLU36_07350"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HMZ46603.1"
                     /translation="MNFNFSERIKSELAEIEAASLFKKERIITSEQGAEIVVNGKTVL
                     NFCANNYLGLSSHPKVIEAAHKAIDSHGYGMSSVRFICGTQDIHKTLEQKIAQFLGTE
                     DTILYAAAFDANGGVFEPLFNEQDAIISDALNHASIIDGVRLCKAQRYRYEHNNMEDL
                     ENKLKETANLRNRIIVTDGSFSMDGTIAQLDKICDLADKYDAIVMIDECHSSGFLGKT
                     GRGTHEYRNVMGRIDIITGTLGKALGGASGGFTSGKKEVIEMLRQRSRPYLFSNTVAP
                     SIVGASIAVLDMLTETTELRDKLEFNTKYFRSKMTEAGFDIKPGDHPIVPIMLYDAVV
                     AQNFASKLLDEGIYVIGFFYPVVAKGLARIRVQLSAAHQQQHLDKAINAFIKIGKELN
                     VLKG"
     gene            3603..4412
                     /gene="blaOXA"
                     /locus_tag="PLU36_07355"
     CDS             3603..4412
                     /gene="blaOXA"
                     /locus_tag="PLU36_07355"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HMZ46604.1"
                     /translation="MRSLITVIVLFSTILVACSPNNIKEDKSLKKYFEENKVEGTFGI
                     FDNGKGDFTIYNLGRYKDSAFTPASTFKIVNALIGLQTGAITNEKMVIKWDGKKRSIA
                     EWNKDLTMEEAFKISAVPYFQEVAKKIGKDTMQIWLDTLGYGTKKINTAIDTFWLDNS
                     LKITADEQLGLIKKLYFNQLPFNKTTQEKVKDVMLQEKNSNYILAYKTGWGTKENGKD
                     LGWVVGWIEENKHPHFFVLNIEGEALMDMVNIRKNILIKILNHLGYLQGKQ"
     gene            4455..5315
                     /gene="tatC"
                     /locus_tag="PLU36_07360"
     CDS             4455..5315
                     /gene="tatC"
                     /locus_tag="PLU36_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HMZ46605.1"
                     /translation="MLGFLNRRNGNKDTSEMTFIDHLEALRAHIVRSIIAIFLIAIAI
                     FIFRDWVFDNIITGPINPDFISYRALCNFSHWLGIGDVLCMPPVKVEMQTTTFGGQFI
                     SSITIAFVGGFIVAFPYVFWEFWRFIKPALQEKEVKNTRFAIFWVSFFFFTGAAFGFF
                     LLGPFTFNFLAGFQLGTHHVLVTRPTLNDYIDNLVNILLGCGIAFELPVLSYILTKIG
                     IITPSFLKKTRKYAVVLILVAAAVITPSPDWMSQMLVFIPLWMLYEISIIISSRVFKE
                     DLKKEQEEWG"
     gene            5346..5801
                     /gene="rpiB"
                     /locus_tag="PLU36_07365"
     CDS             5346..5801
                     /gene="rpiB"
                     /locus_tag="PLU36_07365"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HMZ46606.1"
                     /translation="MNPHFNLSLPIAIGCDHAGVDYKIAIKNYLQQKGYVVNDFGTNT
                     KDSVDYPDYAHAVANSVESNLVAFGILLCGSANGVCITANKHQQVRAGLAWNNDVAKL
                     IRQHNNANIICLPARFIALPLALEMIDNFITTAFEGGRHENRVNKIACC"
BASE COUNT         2178 a          780 c          991 g         1972 t
ORIGIN      
        1 atactaaacg tcctaatgtt cctgtacaca tggatatgcc aggttgggca aacacagcag
       61 gattttattc taaactggag gggaaaatga ataatcataa ttttttaata aacttaaatg
      121 cacattacaa tacttcattg gctgaaatga ctatgtactc aaaaaacagc aacgaaaaag
      181 atatgtttat gcttacttgg cctggtgttc ataccatatt tggaaaatta tttgtagaag
      241 ataaaattga tttgaacaaa aactggagtg caaatattag tgcaggatta agtataaaca
      301 acaacaccat aaaaaacgaa ttaggcttaa caagtttgca aatattttat cctaatttat
      361 tagcatcaaa aacaaggttg cttaaaaaca ttgcaggaaa aatatattat cgtaaaaatc
      421 aatggcaaca aagctttgga gtaggttttg gcgaaagagc accatccatt tctgaaggct
      481 atggctttta tttattcaac agttttgaca ggtacgatta tataggaaat ccaactttga
      541 aaaatgaaaa atctttagaa ataaattcat caacatctta taacacaaaa attctttcta
      601 tacattttca agcagattat tttagaattt ttaattacat tattggtatg cccaaccata
      661 atttcatacc catgactatt ggtgcaaatg gtgtaaaagt gtatcaacaa cttccttatg
      721 taaatatttt taaccttagt tttagttcta catatcgtat tacaaattcg atacaaattg
      781 caggaaaaat ggtgtacaga aaaggaaatt acgaatcgtt gaatttacca caaatacaac
      841 ctataagtta tggcagcact atacgttatc aacacaaaaa ttttatagca gaatgtacag
      901 ttgaaggtgc attaaaacat acaaacttta gcccaacatt tggtgaaact ggaaccgatg
      961 attatactat tttaaaccta tctgcttcta aacaaattaa aatatttaac caaacagtac
     1021 aaataaaaac aggctttgaa aatttgtttg atagatatta ttcaaccttt gcagattgga
     1081 acaaaatacc aagaaatgga agaaattttt tcattaatgt agtgtataat ttttaataaa
     1141 atgcagccta ttttattatg cagttttatt gattagtttt gcggctcttt ttaaaaacat
     1201 aaaatatgct ttgcatttat catcaatcta ctgatcctta ttttaacata gctacagacg
     1261 aatatatttt aaaacatatt gaagaagatt gtttcatgct ttggcgaaac gataatgcaa
     1321 ttattgtagg caaacatcaa aatgccttag cagaaataaa ttacgaatat gtaaaacaaa
     1381 ataacattca tgtggtaaga aggctttctg gtggtggtgc agtttaccat gatttaggca
     1441 acttgaattt ttcttttacc aagcaaggaa aaacttctga aatggtagat tttaaattat
     1501 acacacaacc aattattgag gttttacata gtttaggtat tgaggctaag tttgaaggaa
     1561 gaaatgatat tactattgat ggaatgaaaa tttctggtaa tgccgaacat ttatttaaac
     1621 acaaattact gcatcatggc acacttttat tttcatcgca aatgaaaaat gtaagtgaag
     1681 ctttaaaaat tcatccttta aaatatcagg ataaagctgt aaaatcagta cctaaacgtg
     1741 ttaccaatat ttcgcaacat ttaaaaatac caataacact agaggagttt acacaaaaaa
     1801 ttatgaacca tattgtaagc acaacaccca atgctaccct atatgaattt acaaaagatg
     1861 attttaaaaa aatagaagcc attaaacaag aacgttatgt aaaagaagat tggaactttg
     1921 gtttaatagc caattataat tttaataaaa gcatcaaaac caatggtggt atagtagaaa
     1981 tacatttaat tgtagaaaaa ggaataatta aagaagcgaa aatttatggc gattttttta
     2041 atttgaaaga tattgcagaa atagaaaatg cacttcaaaa ttccaggcat gatgaaaact
     2101 caataagaaa aatttttgaa aattttgtta tagatgaata ttttatgaat attaccttag
     2161 atgaaattat tagtgctatg ttttaaaaac caatatagta acagtgtaaa tatacttttt
     2221 ctgctataac ttttaaaatc ctctaatcct caaccttatc tttgtaacct taaattataa
     2281 taacaatgaa tttcaatttt tcagaaagaa taaaatcaga gcttgccgaa attgaagctg
     2341 ccagtttatt taaaaaagaa agaattatta ctagcgaaca aggtgctgag attgttgtga
     2401 atggcaaaac tgttttaaac ttttgtgcta ataattattt aggtttatcc tctcatccta
     2461 aagtaataga agctgctcat aaagcaatag atagccatgg gtatggaatg agcagtgtac
     2521 gttttatttg tggcacacaa gatattcata aaaccttaga gcaaaaaatt gcacagtttt
     2581 taggaacaga agataccatt ttatatgcag ctgcatttga tgcaaatggt ggtgtttttg
     2641 aaccattgtt taatgaacaa gatgctatta taagtgatgc gttaaatcat gcaagtatta
     2701 ttgatggagt tcgtttatgt aaggctcaac gttacagata cgagcacaat aacatggaag
     2761 atttagaaaa taaattgaaa gaaacagcaa atcttagaaa tagaattatt gttaccgatg
     2821 gtagctttag catggatggc accattgcac aattagataa aatttgcgac ttagcagata
     2881 aatatgatgc tattgtaatg atagacgaat gtcattcatc tggcttttta ggaaaaacag
     2941 gaagaggaac acacgaatac agaaatgtga tgggtagaat tgatattatt actggtactt
     3001 taggcaaagc tttaggtgga gcaagtggtg gctttacaag tggtaaaaaa gaagttatag
     3061 aaatgttacg ccaacgttct cgtccatatt tattttcaaa cacagttgca ccaagtattg
     3121 taggtgctag cattgctgtg ttagatatgc ttacagaaac tactgagcta agagataaat
     3181 tagaatttaa tacaaaatat tttagatcaa aaatgacaga agcaggtttt gatattaaac
     3241 ctggcgacca ccccattgtt cctataatgt tatacgatgc agttgtagca caaaattttg
     3301 caagtaagtt actagatgaa ggtatttatg taattggttt cttttatcct gttgtagcaa
     3361 aaggcttagc tcgtattcgt gtacaattaa gtgctgcaca ccaacaacaa catttagata
     3421 aagccatcaa cgcatttata aaaattggta aagaactaaa tgttttaaaa ggctaactgc
     3481 atttttattc ttgaagataa tgcaatgtaa aatcgggatt ttatacccga ttttttattt
     3541 tgaataaaca gtaaaaagca acattttaac ttaaatttgc aaactcaatt aaaaacaatt
     3601 tcatgcgaag tttaataact gtgatcgttt tattttctac tatattagtt gcatgctcac
     3661 caaataatat taaagaagat aagagcttaa aaaaatattt tgaagaaaat aaagtagagg
     3721 gaactttcgg aatttttgat aatggtaaag gagattttac tatttacaat ttaggccgtt
     3781 acaaagattc agcttttact cctgcatcta cttttaaaat tgtaaatgct ttaattgggt
     3841 tacaaacagg ggcaattacc aatgaaaaaa tggtaataaa atgggatggt aaaaaaagaa
     3901 gtattgcaga atggaataaa gatcttacca tggaagaagc atttaaaata tcggcagtgc
     3961 catactttca agaagtagcc aaaaaaatag gaaaagatac catgcaaatt tggttagata
     4021 ctttaggcta cggaactaaa aaaattaata ccgctattga tactttttgg ttagataatt
     4081 ctttaaaaat tactgccgat gagcaattag gcttaattaa aaaactatat ttcaaccagt
     4141 tgccttttaa taaaacaaca caggaaaaag tgaaagatgt aatgctgcaa gaaaaaaatt
     4201 ctaattatat attagcatat aaaacaggtt ggggcactaa agaaaatggt aaagatttag
     4261 gttgggtagt tggttggatt gaagaaaata aacatccaca tttttttgta ttaaatatag
     4321 aaggagaggc tttaatggat atggttaata taagaaagaa tattttaata aaaatattaa
     4381 atcatttagg ctatctgcaa ggcaaacaat aatttgaatt tttattaatt tatatttgaa
     4441 tttaaactta ataaatgcta ggttttttaa atagaagaaa tggaaacaag gatacatctg
     4501 aaatgacatt tattgatcat ttagaagcat taagagcaca cattgttaga agtattattg
     4561 ctatttttct tattgctatt gctattttta tttttagaga ttgggtgttt gataatatta
     4621 ttacaggccc tattaatcca gattttatca gttatagagc cttatgcaac tttagtcatt
     4681 ggttgggcat tggagatgtt ttgtgtatgc ctcctgtaaa agtagaaatg caaaccacaa
     4741 cttttggtgg ccagtttata agcagtatta ccattgcttt tgttggtggt tttattgtgg
     4801 catttccgta tgttttttgg gagttttggc gttttattaa gccagcctta caagaaaaag
     4861 aagttaagaa tacaaggttt gcaatttttt gggtttcctt ttttttcttt acaggtgcag
     4921 cttttggttt ctttttgtta ggtcctttta cctttaattt tttagcaggt tttcaattag
     4981 gtacacatca tgtattggtt acaaggccta ccctaaatga ttatattgat aatttagtaa
     5041 atattttact aggttgtggc attgcatttg aattgccagt attgtcctac attcttacaa
     5101 aaattggaat tataacacct tcttttctta aaaaaacaag gaagtatgca gttgtattaa
     5161 ttttggttgc agctgctgtt attacaccaa gcccagattg gatgagccaa atgctagtat
     5221 ttattccttt atggatgttg tatgaaataa gtataattat ttcttcaaga gtttttaaag
     5281 aagatttaaa aaaagaacaa gaggagtggg ggtaaccttt ttaattgata acaaaataaa
     5341 aaattatgaa tccacatttt aatctttccc taccaattgc aataggttgc gatcatgcag
     5401 gtgttgatta taaaattgct attaaaaatt atttacaaca aaaaggatat gttgtgaatg
     5461 attttggcac caatacaaaa gattctgtgg attatcccga ttatgctcat gcagttgcca
     5521 acagtgtaga aagtaatttg gtagctttcg gcattctgct atgtggtagt gcaaatgggg
     5581 tttgtattac tgcaaataaa caccaacaag ttcgtgcagg cctagcatgg aataatgatg
     5641 ttgcaaaact tataaggcaa cataacaatg caaatataat ttgcttgcca gctcgtttta
     5701 ttgctctacc attagcttta gaaatgatag ataattttat tactactgca ttcgaaggtg
     5761 gaagacacga aaacagggtt aataaaattg cttgctgcta agcaaattct tctatggttg
     5821 ttttagtaat ataatttgaa aaatattatt taaggataaa aaaattctta atggtttatt
     5881 ttatctaatg cttttttgct aatattgtgt agataggtat t
//
LOCUS       DAOQPK010000140         5913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_7989_length_5913_cov_4.676511, whole genome shotgun sequence.
ACCESSION   DAOQPK010000140 DAOQPK010000000
VERSION     DAOQPK010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7989_length_5913_cov_4.676511"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1566
                     /locus_tag="PLU36_07370"
     CDS             <1..1566
                     /locus_tag="PLU36_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ46607.1"
                     /translation="DLNLFSINDIERIEICKGAQSTLYGSDAIAGVINIITIKKEIDK
                     AVNIKATTAFGNKNTSRNNVQVFGKLNNLIYTARFAQLKTDGFSSAYDSAGNKNFDND
                     AYKGNVANAAVQYQITKKLSFKSFVQHSSYKADIDAGVFADKRNYFINNNVLNTGAGL
                     NYKSTTLHVVGNYQYSQTRRHYNDGFAAGLPVYSTNDYNGISNFFEVYASYKINKQFT
                     VLVGSDYRFATMDGAYVSSAWGTSKYKDTSMNQSSVYASVLFNSINNQLNIELGGRLN
                     KHSRYGNNSTFTFNPSYNINKHFRLFASVATGFKSPSIYQLFDTWSGNKDLKAEKSIN
                     YETGVQYADQKFKTRVVYFNRKIDNGIDYNYISFKYFNYIKQTVSGLELEATLQPVKQ
                     VTIGANYTMLSPKETTQNRITNVDTVTYNYLLRRPKNTFSLNIAWQATKQLSLSVNTK
                     YVDDRFDVGGWAKADVKLKNYFMLNAYAEYVLNNSVKFFVDAQNIGNRKFFEVNGYNT
                     MPIMANIGVTFNW"
     gene            1950..2531
                     /locus_tag="PLU36_07375"
     CDS             1950..2531
                     /locus_tag="PLU36_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HMZ46608.1"
                     /translation="MNIETDALLTVTFTLFAVIDIVGSIPLLISLKDKMGGTIRSFQA
                     TIVSGALMILFLFLGKPFLGILGIDVKSFAVGGSIVIFLLGLEMVLGHEIFKGDKDAK
                     AGTVVPIAFPVIAGSGTLTTVMSLKANFNEMVILIGILINLIVIYAALKSLTLIERIL
                     GKAGLLAVRKFFGVILLAIAVKIFSSNIGSFGK"
     gene            2645..3706
                     /locus_tag="PLU36_07380"
     CDS             2645..3706
                     /locus_tag="PLU36_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine monophosphate reductase"
                     /protein_id="HMZ46609.1"
                     /translation="MSSVALTYDDIQLVPAYSNISSRKNIDLTTRVTKNYGLKMPLVA
                     SCMDTVCESEMAIAIAEMGGVGCIHRFMSIEQQAIEVDIVKKALQETQVINFWNVGNA
                     DWKYTKAIPIMAATGANGDFKERALALANAGANIILIDVAHGHHENVKQAIATLKNSL
                     PKYVDIIAGNIASAQAAKDLEAWQADGLRVGVGGGSLCTTRLKTGFGVPNVSCLQEII
                     AVTALPVMADGGIRTSGDIAKALALGSSCVMLGSLIAGTKEAPGKIIEKPTGLYKRYR
                     GAASLETKMVHNQEQRNVEGESTVIPFKGGAKFVIEGLLDGLKSAFSYGGASNIRDFK
                     PNYVQVTHAGQTEAKPHLL"
     gene            3771..4322
                     /locus_tag="PLU36_07385"
     CDS             3771..4322
                     /locus_tag="PLU36_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HMZ46610.1"
                     /translation="MSQTNQQYDLIINSCKDVFIKKATDYGTAWRVLRLISVVDQIFI
                     KALRIRTIQDLKQQKVKDNITDEFKGIINYGVIGLIQLELKESKIEELATTYVQTLYS
                     KQITLSKDTMLNKNHDYGEAWRDMSQESFTDLILMKLQRIKQILANDGKTIISEGIDA
                     NFVDIINYSVFALILISEKETNH"
     gene            4432..5544
                     /gene="mnmA"
                     /locus_tag="PLU36_07390"
     CDS             4432..5544
                     /gene="mnmA"
                     /locus_tag="PLU36_07390"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HMZ46611.1"
                     /translation="MSKKGKVLVAMSGGIDSTVCALMLHHEGYEVIGITMKTWDYATS
                     VSTNSKKETGCCNVDSFNDARMAAVHHGFPHFILDIRDEFGDFVVENFVEEYLAGRTP
                     NPCVMCNTHIKWKALLKRADALDCDFIATGHYAQIQQHNNNRYYISKGLDETKDQSYV
                     LWGLGQELLSRTIMPLGKYHKTEIRQMANDMGYPELAKKSESYEICFVPDNDYRGFLK
                     RKVDGLEQKVAGGWFIDKTGKKLGKHKGYPFYTIGQRKGLDIALGKPAYVTAINPNTN
                     TVVLGDEIDLEKNTMQVNKINWIKYNGISDGMEALTKIRYKDKGAFSNLYNNENGIKV
                     QFYEPVKSIAPGQSAVFYEGDDVIGGGIIQSNIFNF"
     gene            5623..>5913
                     /locus_tag="PLU36_07395"
     CDS             5623..>5913
                     /locus_tag="PLU36_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46612.1"
                     /translation="MKLFVRYSVLIFSLFFVACSSKETINLPTVAELNPLQNGKVFIY
                     RLDSTVITNFSKSLAIKSYHAKDSIADNFVDVSGKQSFKIYRYITDTLQTNPW"
BASE COUNT         2145 a          799 c         1045 g         1924 t
ORIGIN      
        1 gacctgaatc ttttttctat taatgatatt gaaagaatag aaatttgtaa aggagcacaa
       61 agcacattat atggcagcga tgctattgct ggtgttataa acattataac tataaaaaaa
      121 gaaattgata aagcagtaaa tattaaagct acaaccgcct ttggtaataa aaatacaagc
      181 agaaataatg tacaagtttt tggcaagctt aacaatctta tctacactgc acgttttgct
      241 caactaaaaa ccgatggatt ttcgagtgct tatgatagtg ctggaaataa aaattttgat
      301 aacgatgcct acaaaggaaa tgttgctaat gctgcagtac aataccaaat aacaaaaaaa
      361 ttatctttta aatcttttgt gcaacacagt agctataaag cagatattga tgcaggcgtt
      421 tttgctgata agagaaatta ttttattaat aacaatgttt taaatactgg tgcagggtta
      481 aattataaaa gcactacact acatgttgta ggtaattatc agtacagcca aacacgcagg
      541 cattataacg atggctttgc tgctggttta cctgtttact ctactaatga ttataatggt
      601 atttctaact tttttgaagt ttatgcttct tataaaataa ataaacaatt tacggtattg
      661 gttggtagcg attatcgttt tgcaactatg gatggagctt atgtttcatc tgcatggggt
      721 acaagtaaat acaaggatac cagcatgaac caaagttctg tttatgcttc ggtattattt
      781 aatagcataa acaatcaatt aaatattgag cttggcggaa ggttaaacaa acattctcgt
      841 tatggcaaca atagcacttt tacttttaac ccatcttaca atatcaataa gcatttcaga
      901 ttatttgcaa gcgttgcaac aggatttaaa tctcctagca tttatcagtt gtttgataca
      961 tggagtggta acaaagactt aaaagccgaa aaatctatta actacgaaac aggtgtacaa
     1021 tatgccgacc aaaagtttaa aacaagagta gtatatttta acagaaagat tgataatggc
     1081 attgattata attatattag ttttaaatat ttcaactata tcaaacaaac tgtatctggt
     1141 ttagaattag aagcaacttt acagcctgta aaacaagtaa ctattggtgc taattatact
     1201 atgctttctc caaaagaaac aacacaaaat agaattacaa atgtagatac ggttacttac
     1261 aattatttat tacgtcgacc aaaaaacact ttctctttaa atattgcctg gcaagctact
     1321 aagcagttaa gcttatctgt aaatacaaaa tatgtagatg acagatttga tgttggtggt
     1381 tgggcaaaag cagatgtgaa attgaaaaac tattttatgt tgaatgctta tgctgaatat
     1441 gttttaaata actcggttaa attttttgta gatgcccaaa atattggcaa caggaaattt
     1501 tttgaagtaa atggctacaa cacaatgcca ataatggcaa atattggtgt aacatttaat
     1561 tggtaattta ttttatttag ctttagaaaa tgaaagagca gttgataaaa atttgtttta
     1621 aatgtaaaag caaatttgta tgtaacagca ataatattaa ggcttgtaac tgcacatcta
     1681 tacaactaag taatgaagaa agtagttata tcaaaacaaa gttttctgac tgtttatgca
     1741 ataattgttt aaatatttta aagcaggagt atagacagca gtaaattttt ttagttattt
     1801 aaactaattc ttttaaagag gaactttata tctgaagttc ctcttttttt aaatttagaa
     1861 caataaggta tttaatagaa tgtttaatta aagtcaactt tactcactca tattatttaa
     1921 gaagttatat ttttgaaaaa agcaatgaaa tgaatattga aacagatgca ctactaacag
     1981 taacttttac tctttttgct gtaatagata tagtaggatc aattccatta ttaatatcct
     2041 taaaagataa aatgggtggt actattcgtt cttttcaagc tactatagta tctggtgctt
     2101 taatgatttt atttttattt ttaggaaaac cttttttagg tattttagga attgatgtaa
     2161 aaagttttgc tgttggagga tctatagtta tttttttact gggcttagaa atggttttag
     2221 gccacgaaat ttttaaagga gataaagatg ctaaagctgg tacagttgta cccattgctt
     2281 ttcctgtaat tgcaggtagt ggcacactta ctactgtaat gagcttaaag gccaacttta
     2341 acgaaatggt tattttaatt ggtattttaa ttaacctaat agttatttat gctgccttaa
     2401 aatcattaac attaatagaa agaattttag gcaaagctgg cttattggct gtgcgtaaat
     2461 tttttggagt aattttatta gcaatcgctg taaaaatatt tagctcaaat attgggtcat
     2521 ttggtaaata atttttaaag taattcactt ttaatacaca attttggtaa gccattttta
     2581 tagctgtagt attatgttta ctttgcttga ttgaaagtaa attattaata caataaattg
     2641 gcttatgagt tctgttgctt taacttatga tgatatacag ttagtacctg cttattcaaa
     2701 catatcttct cgaaaaaata ttgatttaac cacaagggtt accaaaaatt atggtttaaa
     2761 aatgccattg gttgcaagtt gtatggatac agtttgcgaa agtgaaatgg ctattgcaat
     2821 tgccgaaatg ggtggtgttg gttgcataca caggtttatg agtatagaac aacaagccat
     2881 agaagtggat atagttaaaa aagctttaca agaaactcaa gtaataaact tttggaatgt
     2941 aggcaacgcc gactggaagt acacaaaagc tattcctata atggctgcaa caggtgctaa
     3001 tggtgatttt aaagaacgtg ccttggcatt agccaatgca ggggcaaata ttattttaat
     3061 agatgttgcc catgggcatc atgaaaatgt aaaacaggct atagcaacat taaaaaatag
     3121 tttacctaag tatgtagata taatagctgg taatattgca tctgcacagg ctgcaaaaga
     3181 tttagaagca tggcaagcag atggcttaag agttggtgtt ggtggtggct ccttatgcac
     3241 tacacgttta aaaacaggct ttggagtgcc taatgtaagt tgcttacaag aaattattgc
     3301 tgttactgcc ttacctgtta tggcagatgg tggtataaga accagtggcg atatagctaa
     3361 agcattggca ttaggtagca gctgtgtaat gttaggttct ttaatagctg gtacaaaaga
     3421 agccccaggt aaaataattg aaaaaccaac aggcttatac aaacgttata gaggagcagc
     3481 ctctttagaa actaaaatgg tacataacca agaacaaaga aatgtagaag gagaaagtac
     3541 tgtaatacct tttaaaggtg gagcaaaatt tgtaatagaa ggtttgttag atgggttaaa
     3601 aagtgccttc tcttatggtg gggcaagtaa cattagagat tttaaaccaa attatgttca
     3661 ggtaactcat gctggtcaaa cagaagccaa acctcatttg ttataaatat tttttatttc
     3721 agaattatat tattttacca ctaatcttgc agcactgaaa aagaaaataa atgagccaaa
     3781 ctaatcaaca atacgattta ataataaata gttgtaaaga tgtttttatt aaaaaagcaa
     3841 cagattatgg aacagcctgg agagtattaa ggctgattag tgttgtggac caaattttta
     3901 ttaaggcttt acgtattaga accattcaag atttaaaaca acaaaaagta aaggataata
     3961 ttacagacga gtttaaagga attattaatt atggtgtaat tggtttaata caattagaac
     4021 ttaaagaatc taaaattgaa gaattagcca ccacttacgt acaaactttg tatagcaagc
     4081 aaataacatt gtcaaaagat accatgctaa acaaaaacca tgattatgga gaggcttgga
     4141 gagatatgag tcaggaaagt tttacagatt taattttaat gaagctacaa cgcataaaac
     4201 aaatacttgc caatgatgga aaaaccatca tcagcgaagg tatagatgcc aactttgttg
     4261 atataattaa ctatagtgtt tttgccctca ttcttatatc tgaaaaagaa acaaatcatt
     4321 aattccttcc gttattttat taaaaaaatt gcccatcttt cattacttcc ttgtttcaaa
     4381 aactacattt tcaataaacc tgtaattttg taggttcttg tttaaaaaat aatgagtaaa
     4441 aaaggaaaag tattggttgc aatgagtggt ggtattgata gtactgtttg tgctttaatg
     4501 ctacatcacg aaggttatga agttattggc attacaatga aaacttggga ttatgctaca
     4561 agtgttagta ctaactctaa aaaagaaact ggttgttgta atgtagatag ttttaacgat
     4621 gccagaatgg ctgctgtgca tcatggattt cctcatttta tattagatat tagagatgag
     4681 tttggcgatt ttgttgtaga aaattttgtt gaagaatatc tagctggccg tacacctaat
     4741 ccttgtgtaa tgtgcaatac ccatataaag tggaaagcat tattaaaacg tgccgacgct
     4801 ttagattgtg attttattgc aacagggcat tatgcacaaa ttcaacagca taataacaac
     4861 aggtactata ttagtaaagg cttagatgaa actaaagacc agagttatgt actatgggga
     4921 ttaggccaag aactacttag cagaacaata atgccattag gcaaatacca taaaaccgaa
     4981 attaggcaaa tggcaaacga tatgggctat cctgaacttg ctaaaaaaag tgagagctac
     5041 gaaatttgtt ttgtgccaga taatgattac cgaggttttt taaaaagaaa agtggatggc
     5101 ttagaacaaa aagtagcagg tggatggttt atagataaaa caggaaaaaa acttggcaaa
     5161 cataaaggct acccctttta taccattggg caaagaaaag gattggatat agcattaggc
     5221 aagccagcct acgtaacagc cattaacccc aataccaata ctgttgtttt aggagatgaa
     5281 attgatcttg aaaaaaacac catgcaagta aacaaaataa attggataaa atacaatggc
     5341 atatcagatg gcatggaagc tttaaccaaa ataagatata aagataaagg tgcttttagc
     5401 aacttatata ataacgaaaa tggtattaaa gtgcagtttt acgaacctgt taaaagtatt
     5461 gcccctgggc aaagtgcagt tttttatgaa ggagatgatg taattggcgg aggaattata
     5521 caaagcaata tttttaactt ttaactatac cacactaaat taagttagtg tatcttgttt
     5581 tgtaaattca tattttatat atctttccac ccaacttagt ttatgaaatt gtttgtaaga
     5641 tatagtgttt taatttttag tttatttttt gtagcctgta gctccaaaga aacaataaac
     5701 ctgccaacag ttgctgaatt gaacccctta caaaatggca aagtttttat ttacagatta
     5761 gattcaacag taataactaa tttcagtaaa tcattggcca ttaaaagtta tcatgcaaaa
     5821 gatagtattg ccgataactt tgttgatgta agtggaaaac aatcttttaa aatttataga
     5881 tatattaccg atacattaca aactaaccct tgg
//
LOCUS       DAOQPK010000141         5889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_8041_length_5889_cov_5.015941, whole genome shotgun sequence.
ACCESSION   DAOQPK010000141 DAOQPK010000000
VERSION     DAOQPK010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5889
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8041_length_5889_cov_5.015941"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="PLU36_07400"
     CDS             <1..886
                     /locus_tag="PLU36_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ46613.1"
                     /translation="LGVVFLIVAAFFKFSSIPNKIDLDHVAEEEADSNDKIMHKKSAF
                     QYPQLWLGMIGIFVYVGVEVATASNLPEYLKVKMGIGTEKVAPYISLYWASLMIGRWT
                     GSLEAFNFKKGTKDILKFLMPYIAFGVFLLVNKIAQHDITPFYVYAFVIIAMIIGDIL
                     SKGNPARMLLIFSAMGIIALLIGMFTHGMVSVFAFISVGLFCSTLWPCIFTLAVAGLG
                     KHTNEGSSYLIMMIMGGGFISYLQGYVAADHLLGIQYSYIVCVACFVYLAFFAIAVKR
                     ILQKQGIDYDTNTADIKH"
     gene            complement(883..1518)
                     /locus_tag="PLU36_07405"
     CDS             complement(883..1518)
                     /locus_tag="PLU36_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HMZ46614.1"
                     /translation="MPLFYQQNINQFTRLAIWKIEESESFFSNTVPLQNSITHNHKRL
                     QHLAGRFLLKYLYPSFPYHAIEIADTKKPFLPNEDFHFSISHCGDYAAAIVSTKNRVG
                     LDIELSTDKINRIASKFLNETEFKNNAINLQEITVAQQNKLTLLWSAKEAIFKWWGNG
                     KVDFSEMIKTNSFVFSENGTIPIQFKNHLLQAHYKQLNNMYLVYVISDINT"
     gene            1590..2126
                     /gene="dcd"
                     /locus_tag="PLU36_07410"
     CDS             1590..2126
                     /gene="dcd"
                     /locus_tag="PLU36_07410"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HMZ46615.1"
                     /translation="MILTDKRILEEMDKGTIKIVPYNRADLGSNSYDVHLGSTLATYI
                     DKELDAKKHNTIEYFEIPDEGYVLQPHAFYLGVTLEYTETHAHVPFLEGKSSTGRLGI
                     DIHATAGKGDVGFCGNWTLEISVKQPVRIYKGMPIGQLIYFPVEGEIEIKYNEKRNAK
                     YSGQPNKPIESMMWKNKF"
     gene            complement(2127..3650)
                     /locus_tag="PLU36_07415"
     CDS             complement(2127..3650)
                     /locus_tag="PLU36_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HMZ46616.1"
                     /translation="MSPIVLFSFVIGYFLLLLAVAWYTSKNANNDSFFIGNKNSNWML
                     VAFGMVGTSLSGVTFVSVPGTVGSAGFQYFQVVIGYCIGYLAVAYILLPLYYKMNLTS
                     IYNYLEHRFGNYSYKTGALFFILSRTLGATARLYLVINILQLFILDSMGVSFWVTTLI
                     ILALIVLYTFEGGVKTIVYTDTLQTTCMLLGLLVCIVAIMSKMDLSLSNSIHALSDKG
                     MLQIFNTDIHSSGFFLKQIIGGAFITITMTGLDQEMMQKNISVRTLKDSQKNVLTLSV
                     ILFFVNLLFLFMGGLLYLYAGQNGITSKGDDLFPFIALNSHFSVAVAVIFIIGLISAL
                     FPSADGALTALTSSFCIDILGLKKKTQFTEQQKKRTRLTVHFTFTIIFFLCVLAFKWI
                     NDKSIIDVILKVAGYTYGPLLGLFAFGIFSKRKINDKLSVVVCLIAPVLVIGIDIINN
                     SEWFIHKLNITGSIANSLQNLSQQIFNGFRIGIELLIINGLFTFIGLYIISKPAPKS"
     gene            complement(3707..4114)
                     /locus_tag="PLU36_07420"
     CDS             complement(3707..4114)
                     /locus_tag="PLU36_07420"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HMZ46617.1"
                     /translation="MFESTTQLRVRYAETDQMNVVYYGNYAQYFEVGRAESIRNLGVT
                     YKQLEETGVIMPVVEMHVKYLRSAHYDDLLTIKTMIKELPTGHKVEFHQEVYNEANKL
                     LTIGKVVLYFIDGATKQKTAIPTIFKDKLEKFF"
     gene            complement(4124..4753)
                     /locus_tag="PLU36_07425"
     CDS             complement(4124..4753)
                     /locus_tag="PLU36_07425"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HMZ46618.1"
                     /translation="MRALVLVLVISIFSLNVGYTQNDVEAKKVLDAVSTKVKSFSAIT
                     GNFVIKTITSKGKLNGTKSGTISMKGSKYVLKQGKTQIICDGNNTYSYDGSKTITVAA
                     ADDADKTLTPQKIIAGSYSKDFTYKLVSSKGNFNEIELKPIDKRKNFQKVNVFVDKVK
                     SMITKAKVLDKSNNTLEFSFTNLNTNAKLADKIFVFNKANYPKDVEVLD"
     gene            4890..5885
                     /locus_tag="PLU36_07430"
     CDS             4890..5885
                     /locus_tag="PLU36_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ46619.1"
                     /translation="MHYVSAENLSKSFGVQPLFQNISFNINEGDKIALIARNGVGKST
                     LLKILAGKETTDTGKIYINKEVTTVLFEQNPAFEEDKNIVDNIFKANHPIVNAIRAYE
                     LALEENNNEELLRLATLMDELNAWNFEVKVKQILTKLNIHHLQQTVKNLSGGQRKRIA
                     LAKTLIEIGFEHTHTLLLMDEPTNHLDVEMIEWLEKYLNQENVTLILVTHDRYFLDAI
                     TDEIWELENDNLYIYKGDYENYIEKKAARIESDLASIDKAKNQYRKELEWMRKQPKAR
                     TTKSKSRQDNFYEIEAKAKQKIEDAQVQLQVKMSRLGGKIAEMKKVYKSYGTILF"
BASE COUNT         2175 a          849 c          885 g         1980 t
ORIGIN      
        1 gttgggtgta gtgtttttaa ttgttgctgc atttttcaaa ttttcatcta ttccaaataa
       61 aatagattta gatcatgtag cagaagaaga agcagatagt aacgataaaa taatgcacaa
      121 gaaaagtgca tttcaatacc ctcaactctg gttaggtatg ataggaattt ttgtttatgt
      181 tggtgtagaa gtggctactg caagtaattt accagaatat ttaaaagtta aaatgggtat
      241 tggaaccgaa aaagtagccc cttatatttc tttgtattgg gcaagtttaa tgattggtag
      301 atggacagga agtttagaag catttaactt taaaaaaggt acaaaagata ttttaaaatt
      361 tttgatgccc tatatagcat ttggagtttt tttattggta aacaaaattg cccaacatga
      421 tataactcct ttttatgtat atgcatttgt tattatagcc atgataattg gcgacatttt
      481 aagtaaaggc aatcctgcca gaatgttact tattttttct gcaatgggca ttattgcctt
      541 actaattggc atgtttacac atggtatggt tagtgtattt gcatttataa gtgtaggctt
      601 attttgtagt actttgtggc cttgtatttt tacattagca gtagcaggtt taggcaaaca
      661 cacaaacgaa ggaagtagct atttaattat gatgattatg ggtggtggtt tcataagcta
      721 tttacaaggt tatgtagctg ccgatcattt attaggtatt caatacagct atattgtatg
      781 cgttgcttgc tttgtttatc ttgcattttt tgcaattgct gtaaaaagaa ttttacaaaa
      841 gcaaggaata gattatgata ctaatactgc agatataaaa cattaagtat ttatatcaga
      901 aataacgtac accaggtaca tattatttaa ctgcttgtaa tgtgcttgta ataaatggtt
      961 tttaaactga attggtattg tgccattttc agaaaaaaca aaggaattgg tttttatcat
     1021 ttcactaaaa tcaactttgc cattacccca ccatttaaaa attgcttctt tagcactcca
     1081 taaaagtgtt aatttatttt gttgtgcaac ggtaatttct tgtaggttaa ttgcattgtt
     1141 tttaaattct gtttcattta aaaacttaga ggcaattcta ttaattttat cagtacttaa
     1201 ttcaatatct aaacctaccc tattttttgt actaacaatt gctgcagcat aatctccaca
     1261 atgagaaata gagaaatgaa aatcttcatt tggcaaaaat ggtttttttg tatccgcaat
     1321 ttcaattgca tgataaggaa aactagggta taaatacttt aataaaaatc tacctgctaa
     1381 atgctgtaac cttttatggt tatgtgttat agagttttgc aaaggaacag tattactaaa
     1441 aaaagattct gattcttcta tcttccaaat tgcaagcctt gtgaattggt taatattttg
     1501 ttgataaaac aaaggcatat acaaatttgt tttttaatgt ttgtgcttga agattaattt
     1561 tatttgcaaa taaagtttat tattacaata tgatactaac agataaaaga attttagaag
     1621 aaatggataa aggaactata aaaattgttc catataacag agccgattta ggtagcaaca
     1681 gttatgatgt acatttaggc agcactttag caacctatat agataaagaa ttagatgcaa
     1741 aaaaacacaa tacaattgag tattttgaaa taccagatga aggatatgtt ttacaacctc
     1801 atgcatttta tttaggggta actttagaat atacagaaac ccatgcccat gtaccttttt
     1861 tagaaggcaa aagcagcact ggtcgtttag gaatagatat acatgctact gcaggcaaag
     1921 gcgatgttgg tttttgtggc aactggactt tagaaatatc ggtaaaacaa cctgtaagaa
     1981 tttataaggg aatgcctatt ggacagttaa tttattttcc ggtagaaggc gaaattgaaa
     2041 taaaatataa cgaaaagaga aatgcaaaat atagtgggca gcctaacaag cccattgaaa
     2101 gtatgatgtg gaagaataaa ttttaattaa cttttgggtg ctggtttaga aattatatac
     2161 aatcctataa aagtaaataa gccatttata attaataatt ctattccaat tctaaaacca
     2221 ttaaaaattt gttgcgataa gttttgtaaa ctatttgcaa tgcttccagt aatattcaat
     2281 ttatgaataa accattctga attatttata atatcaatac caattaccaa tactggtgct
     2341 attaaacaaa caacaacaga aagcttatcg tttatttttc ttttagaaaa tattccaaat
     2401 gcaaacaagc caagtaaagg cccatacgta taaccagcta cttttaaaat tacatctata
     2461 atagatttat cgttaatcca tttaaaggcc aatacacata aaaagaaaat aatagtaaaa
     2521 gtaaaatgca cggttaacct agttcttttt ttctgttgtt cagtaaactg agtcttcttt
     2581 tttaaaccaa gtatatctat acaaaatgaa gaagtgagag cagtcaaagc tccatcggca
     2641 ctaggaaaaa gagcagaaat taaacctata ataaaaatta ctgcaaccgc aacagaaaaa
     2701 tgactattta aggctataaa cggaaataag tcatcgcctt tagaagttat accattttgc
     2761 cctgcatata aatacaataa acctcccata aacaagaaca aaagatttac aaaaaataaa
     2821 ataacactaa gcgttaaaac atttttttga gagtctttta atgttcttac agaaatattt
     2881 ttttgcatca tttcctgatc aagccctgtc atagtaattg taataaaggc tcctccaata
     2941 atttgtttaa gaaaaaatcc agaggaatgt atatctgtat taaaaatttg gagcatgcct
     3001 ttatcactca aagcatgtat gctattgctt aggcttaaat ccattttgct cattatggca
     3061 acaatacaaa ctaataagcc taatagcata caggttgttt gtaaggtatc ggtataaaca
     3121 attgttttta caccaccttc aaaagtgtat agtacaatga gtgctaaaat aattaaagta
     3181 gtaacccaaa aagaaacacc catagagtct agaataaaca actgtaaaat atttattact
     3241 aaatatagcc ttgcagttgc acctaatgtt ctggataaaa taaaaaataa agcccctgtt
     3301 ttatagctgt aattaccaaa cctatgttct aaataattgt aaatagaagt aaggttcatt
     3361 ttataatata agggcaataa aatataggca actgccaagt atccaataca atatccaata
     3421 actacttgaa aatactgaaa ccctgcacta ccaactgttc caggcacact tacaaaagta
     3481 acgccactta aacttgtgcc taccattcca aaggcaacaa gcatccaatt actgttttta
     3541 tttcctataa aaaacgaatc gttattggca tttttactag tgtaccatgc tactgctagt
     3601 aataataaaa agtaacctat tacaaaagaa aataaaacaa ttgggctcat gtatagtgtt
     3661 atgtttgatt aataaacaat acacaaactt taggtatttt caacaactaa aaaaactttt
     3721 ctagtttatc tttaaaaata gtaggaattg ctgttttttg tttggtggca ccatctataa
     3781 aatataaaac tactttacca atagttaata gtttattagc ctcattatat acctcttgat
     3841 gaaactcaac tttatgccct gttggtaatt ctttaatcat agtttttatg gtcaacaaat
     3901 catcataatg tgccgaacgt agatatttta catgcatttc cactactggc attattactc
     3961 ctgtttcttc taattgttta taggtaactc ctaaatttct tatactttct gctcttccaa
     4021 cttcaaagta ttgagcataa ttaccataat aaaccacatt catttggtca gtttcggcat
     4081 atcttactct aagctgtgta gtactttcaa acatgaaaaa aaattaatct aaaacttcaa
     4141 catctttagg ataattggct ttgttaaaaa caaaaatttt atcagcaagt tttgcattag
     4201 tattaaggtt ggtaaaacta aattcaagag tattattgct tttatctaac acttttgctt
     4261 tagtaatcat tgattttact ttatctacaa aaacatttac tttctgaaaa ttctttcttt
     4321 tatcaatcgg cttcaattca atttcattaa aattgccttt actagaaact agtttataag
     4381 taaaatcttt gctgtaacta ccagcaataa ttttttgtgg agttaaagtt ttgtctgcat
     4441 catcagcagc tgctacagta atggttttgc tgccatcgta gctataagta ttattaccat
     4501 cgcaaataat ttgtgtcttt ccttgtttta aaacatattt actgcctttc attgaaatgg
     4561 tgccagactt tgtgccattc aattttcctt tactagttat tgttttaatt acaaaatttc
     4621 ctgtaatagc actaaaagat tttacttttg tactcactgc atctaacact ttttttgctt
     4681 ctacatcatt ttgagtatat ccaacattta aagaaaaaat agaaataact aaaactagta
     4741 ctaatgctct catcttaaat tttaaatttt atggtggcaa atatagtgcc tacacatttt
     4801 ttgatgcctt aaacttataa aggtttaaca aaacataggt ttttagttaa aaaaataggg
     4861 caaaatctaa actctattat cttcgccaca tgcattatgt atcagccgag aatttaagta
     4921 agagctttgg tgttcagcct ttatttcaaa atatttcatt taatattaac gaaggagata
     4981 agattgcttt aatagccaga aatggagttg ggaaaagcac attattaaaa attttagcag
     5041 gtaaagaaac taccgatact ggaaaaattt atattaataa agaagtaact actgttttat
     5101 ttgaacaaaa tcctgctttt gaagaagaca aaaatattgt agataatata tttaaagcta
     5161 accatcctat agtaaatgct ataagagctt atgaacttgc attagaagaa aataataatg
     5221 aagaattatt aagacttgca accttaatgg atgaattgaa tgcatggaat tttgaagtaa
     5281 aagtgaagca aattttaaca aaactaaata ttcatcattt acaacaaaca gtaaaaaatt
     5341 taagtggcgg acaaagaaaa agaattgctt tagctaaaac attgattgaa attggctttg
     5401 aacataccca cactttgcta ttgatggatg aaccaactaa ccatttagat gttgaaatga
     5461 ttgaatggtt agagaaatac ttaaatcaag aaaatgtaac tcttatactg gtaacacacg
     5521 atagatattt tttagacgct attactgatg aaatttggga actagaaaat gataatttat
     5581 atatttataa aggagattat gaaaattata tagaaaaaaa agcagcccgt atagaaagtg
     5641 atttagcaag tatagacaag gctaaaaacc aatatagaaa agagttagaa tggatgcgta
     5701 agcaaccaaa agcaagaaca acaaaaagca aaagcaggca agataatttt tatgaaatag
     5761 aggcaaaagc aaagcaaaaa atagaagatg ctcaagtaca attgcaagta aaaatgagcc
     5821 gtttaggtgg caaaattgcc gaaatgaaga aagtatataa aagctacgga acaatattat
     5881 tttaaatgg
//
LOCUS       DAOQPK010000142         5848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_8132_length_5848_cov_4.933886, whole genome shotgun sequence.
ACCESSION   DAOQPK010000142 DAOQPK010000000
VERSION     DAOQPK010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8132_length_5848_cov_4.933886"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..232
                     /locus_tag="PLU36_07435"
     tRNA            159..232
                     /locus_tag="PLU36_07435"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:193..195,aa:Pro,seq:tgg)
     gene            complement(353..1384)
                     /locus_tag="PLU36_07440"
     CDS             complement(353..1384)
                     /locus_tag="PLU36_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HMZ46620.1"
                     /translation="MSTTKAPTLTAKDFATDQEVRWCPGCGDYSILAQVQKVMPGIGV
                     PKENIVFISGIGCSSRFPYYMNTYGMHSIHGRAAAIASGLKASRPELSVWIISGDGDS
                     MSIGGNHTIHFLRRNFDVNLLLFNNQIYGLTKGQYSPTSEANKITKSTPMGSIDHPFN
                     PLALAMGADATFVARSMDRDPKHLQYVLTRSHQHKGASFVEIYQNCNIFNDGAFETFT
                     EKATKADNTLFLEQNKPLVFGATQNKGIKLDGFKPIIVDLEEGASTNDLWIHDEHDFY
                     KAQILTRMFDDPSIEGHLPRPFGIFYQTQRACYEDVMAMQIEEAIAKKGVGDLNKLLK
                     GREVWEIAS"
     gene            1537..2931
                     /locus_tag="PLU36_07445"
     CDS             1537..2931
                     /locus_tag="PLU36_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HMZ46621.1"
                     /translation="MATKVISVNIHKTIAFIKESFNSEDKDFTTGSIRKAVILLAIPM
                     ILEMSMESIFAVVDIFFVAKLGSYATATVGLTESMLAIIYSIGFGISMATTAMVARRI
                     GEKNYVEAANAAAQSILIAISLSIVITLFGIIYAKELLYLMGATKEIAEQGFLYTAIM
                     LGGNLVIILLFLLNGIFRGAGNAAIAMKSLWLANICNIILCPLFIHYYGLTGAAIATT
                     IGRSIGVIYQLHQLLKGKGFIRLYSKYFKPQLQIIKNLISVTTTATLQFIITSASWIA
                     MARIMTSFGNEAIAGYTIAIRLIVFFILPAFGLSNAAATLVGQNLGAKQPDRAAKSVW
                     KVAKYNAIYMGFVSVLFLFGAETFVKFFAHEIKVIETAVHALRVISLGYIFYGIGMVM
                     MNAFNGAGDTKTPTIVNFFWFWIIQIPMAYIAAKHFNWGEWGVYFAIVITETMVTITS
                     MYLFTKGKWKLVKI"
     gene            complement(3038..4828)
                     /locus_tag="PLU36_07450"
     CDS             complement(3038..4828)
                     /locus_tag="PLU36_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46622.1"
                     /translation="MKKFFYTLIFLCIANISFANFVSPGTGVKWNLDSLVAHSAGIVT
                     FNGAEYIVNDTVFIVTNDTLSITTDAVVKYYPLTYLDVNGVLLINPPNNVKFTAINSE
                     AGFNGMRIDSSNATVLKKLTFEYAVSLKTTDCNITIDSCTFQYNNVGSSTSFGNGALS
                     LFRASPTITHSQFLNNRRAAIQGGANIANAPQIFNNIFYGNGSMNVNVPHINIGATGS
                     DTLRIINNEIIGVPAAIQTGAIGFLPIGSANTIIAGNIIKHNRYGITTNGGSNIYALI
                     KYNIIDSNNIQNNPNLGGSGIAFSGGSATSHQNAIVTGNLIRGNLWGITIQGRSKPNI
                     GNVENVDTTDDGKNVFLQNNNVNTPWTDLYNNTVDSIYAQGNYWGTNIEDTVESRIFH
                     QVDNASLGIVLYSNFILPIRLSSFSISKNNNIFYLNWKTAEELNSDYFEVERSYDGKT
                     FEFVGSVIAKGFASGYSFMDDSKPNVNSSIYYRLKMVDKDGSFMYSKILIIKPAGIKT
                     VENLRIYPSIISSGNNVIWAEFISTKEQPITIQYYTVEGKIVTQLTQNAFKGNNKIII
                     TPNVVLNNGVNYIKVVTEGFEKVIPIVKYN"
     gene            complement(5183..>5848)
                     /locus_tag="PLU36_07455"
     CDS             complement(5183..>5848)
                     /locus_tag="PLU36_07455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ46623.1"
                     /translation="GFISNSTTPSTPTGYYVDMITPNFSGFFLQHAADNLLVPSTCNN
                     FYYKTYNNSVQLFFSTLQENNNSYYQIERSENGENFVPITSFNSNNNINGSKYSYTDY
                     TIKPNAFYYYRIKQVDKDNSYQFICKTILVSTKKVEKTFGKIYPNPVMESFNIDINKP
                     FAGKALVQILNANGQIVYSSTINISLSDQKLNIKTPFISRGVYTLKLLTDKGIEQMQF
                     IKH"
BASE COUNT         1910 a          898 c          895 g         2145 t
ORIGIN      
        1 ggtttgctaa accgtcgtac gggtcaaacc gtaccggggg ttcgaatccc cccctcaccg
       61 ctaatactaa aaagagtctc attatctttt tgttactctt tctccttatt atgaatggag
      121 aaggacgatg gaatgaaaat gagtttcgaa actaccctcg ggaggtagtt cagcccggta
      181 gaatacctgg tttgggacca gggggtcgca ggttcgaatc ctgtcctccc gactttcaaa
      241 aagctcacag tatttgtgag cttttacttt ttgttgcata tttacacaaa atctattcca
      301 aataattatt caacttatta ttgtttaata aaacaagcgt tattattttg gtttaggagg
      361 ctatttccca aacctctcta ccttttaaca atttatttaa atctccaaca cccttttttg
      421 caatagcttc ttctatttgc attgccatta catcttcata acaggctctt tgtgtttggt
      481 aaaaaattcc aaaagggcgt ggtaaatgcc cttcaatgct tgggtcatca aacattcggg
      541 ttagtatttg tgctttataa aaatcatgtt catcatgaat ccataaatca ttagtgcttg
      601 ctccttcttc taaatctaca ataatgggtt taaagccatc taactttatt cctttatttt
      661 gagtagcacc aaaaactaaa ggtttgttct gctctaaaaa caaagtatta tctgcttttg
      721 ttgctttttc tgtaaaagtt tcaaaagcac catcgttaaa aatattacag ttctgataaa
      781 tttctacaaa agaggctcct ttgtgctgat ggcttcttgt taaaacatat tgtaaatgtt
      841 ttggatccct atccatactt cttgcaacaa atgtagcatc ggctcccata gctaaagcta
      901 aaggattaaa tggatgatct atgctaccca ttggagtgct tttggttatt ttatttgctt
      961 cagatgttgg ggaatattgt cctttggtta ggccataaat ttggttgtta aataataata
     1021 agtttacatc aaaatttctt cttaaaaaat gaattgtatg gttgcctcca atgctcatac
     1081 tatcaccatc accactaatt atccaaacac ttaattcagg gcggctagct tttaaaccac
     1141 ttgctatagc tgcagctcta ccatgtatag agtgcattcc ataggtattc atataatatg
     1201 gaaatctaga ggagcaacct attccactaa taaaaacaat attttcttta ggtacaccaa
     1261 ttcctggcat tactttttga acttgtgcta aaatggaata atcgccacag cctgggcacc
     1321 aacgtacctc ttggtctgtt gcaaaatctt tagctgtaag tgtaggtgct tttgtggtac
     1381 tcattgcaaa tttattttat acacaaaggt aaaggttagt gaaattataa aagatacata
     1441 agtttttgaa taaaggtatt attgcttaaa taaatttact gatgaatctt tacatttaat
     1501 tcattttgaa tttatattta tcaactaatt tttttaatgg caaccaaagt tatttcagta
     1561 aatattcata aaactattgc ttttattaag gagtctttta atagtgagga taaagatttt
     1621 acaacaggaa gtattcgtaa ggcagttatt ttattagcta tacccatgat tttagaaatg
     1681 agtatggaaa gcatatttgc tgtggttgac attttttttg ttgccaaatt aggctcttat
     1741 gcaacagcaa cagttggttt aacagaaagt atgctggcaa ttatatactc tattggtttt
     1801 ggtataagta tggctacaac tgcaatggtt gccagaagaa ttggagaaaa aaattatgta
     1861 gaggccgcaa atgctgcagc ccaatctatt ttaattgcta tttcattaag tatagttata
     1921 acattattcg gaattattta tgctaaagaa cttttatact taatgggtgc tacaaaagaa
     1981 atagcagagc aaggattttt gtacactgca ataatgttag gtggcaacct tgtaattata
     2041 ttattgtttt tgttaaatgg tatttttaga ggtgcaggta atgcagctat tgcaatgaaa
     2101 agtttatggc ttgcaaatat ttgcaatatc attttatgcc ctttgtttat acattattat
     2161 ggattaactg gtgctgctat tgcaacaaca attggaagaa gtataggagt tatttatcaa
     2221 cttcatcaac tattaaaagg aaaaggtttt atacgtttat acagtaaata ttttaaacca
     2281 caactgcaaa taataaaaaa tcttataagt gttacaacta ctgcaacact gcaatttatt
     2341 attaccagtg ccagttggat tgctatggct agaataatga caagttttgg aaacgaagct
     2401 attgcaggtt acacaatagc aataagatta attgtatttt ttattctgcc agcttttgga
     2461 ttaagtaatg ctgctgcaac acttgttggt caaaatttag gtgcaaaaca acccgaccgt
     2521 gctgcaaaaa gtgtttggaa ggtagccaaa tacaatgcca tttatatggg ttttgtatcg
     2581 gtgctttttt tatttggagc agaaactttt gtgaaatttt ttgcacatga aataaaagta
     2641 atagaaaccg ctgtacatgc tttaagagta ataagtttag gatatatttt ttatggaatt
     2701 ggaatggtaa tgatgaatgc ttttaatggt gcaggcgata ccaaaacacc aactattgtt
     2761 aattttttct ggttttggat tattcaaatt ccaatggcat atatagctgc aaagcatttt
     2821 aattggggcg aatggggtgt ttattttgca attgttatta cagaaactat ggttacaatt
     2881 acctccatgt atttatttac taagggcaaa tggaaattag tgaagattta gataattttt
     2941 tttgaatttc atttcctaat aaaaatgtca ttgctacaat gtagcaatga cattttaagt
     3001 ttataatgca acttaattaa aacttacctt ctgttattta gttatatttt acaattggta
     3061 ttactttctc aaacccttct gttacaacct taatataatt tacaccatta ttcaaaacta
     3121 cattaggtgt tattattatt ttattgttgc ccttaaaagc attttgtgta agttgtgtaa
     3181 ctatttttcc ttcaactgta taatactgta tagtaatagg ctgctctttg gtgctaataa
     3241 actctgccca aattacattg ttaccagatg aaataattga agggtaaatt cttaaatttt
     3301 ctactgtttt tatacctgca ggttttatta tcaaaatttt tgaatacata aaactgccat
     3361 ctttatctac catttttaaa cgataataaa tagatgaatt aacatttggt ttactatcat
     3421 ccataaaaga gtagccggaa gcaaatcctt ttgcaattac acttccaaca aattcaaatg
     3481 ttttaccatc ataacttctt tcaacctcaa aatagtcgga gtttaattct tctgcagttt
     3541 tccagtttaa ataaaaaata ttgttatttt tagatatact gaaagaggat aatcttatag
     3601 gtaaaataaa gtttgaataa agtactattc ctaaacttgc attatctacc tgatgaaata
     3661 ttcttgactc tactgtatct tcaatattgg ttccccaata attaccttgt gcataaatag
     3721 aatcaacagt attgttgtat aagtcagtcc atggtgtatt tacattattg ttttgtaaaa
     3781 aaacattttt accatcatcc gtagtatcta cattttctac attaccaata tttggtttag
     3841 accttccttg aattgtaata ccccaaaggt tacctctaat taaattacct gttactattg
     3901 cgttttgatg actggttgca gaacctccgc taaaagcaat tccactaccc cctaagtttg
     3961 ggttattttg tatattgtta ctatcaataa tattgtattt tataagagca taaatattag
     4021 aacctccgtt ggtggtaatt ccataacgat tgtgttttat aatattacct gcaataattg
     4081 tattagcaga gccaataggt aaaaacccaa tagccccagt ttgaatagca gcaggaactc
     4141 caataatttc gttattaatt attcttagtg tatcacttcc tgttgctcca atatttatat
     4201 gaggcacatt tacattcata gatccattgc cataaaaaat attattaaaa atttgaggag
     4261 cattggcaat attagcacct ccttgtattg ctgctcttct gttatttaaa aattgtgaat
     4321 gtgttatagt tgggcttgcc ctaaataacg ataaagctcc attaccaaaa cttgtagagc
     4381 tgccaacatt attgtactga aaagtgcaac tatcaatagt tatattacaa tctgttgttt
     4441 ttaaagaaac agcatattca aaagttaatt tttttaatac tgtagcatta ctactatcta
     4501 tacgcattcc attaaaacct gcttcgctat tgatggctgt aaattttaca ttattaggag
     4561 gatttattag caacacacca tttacatcta aatatgtaag agggtaatat tttaccacag
     4621 catctgttgt aatgcttaac gtatcgttgg taacaataaa aacagtatcg tttacaatat
     4681 actcggcacc attaaaagta acaatgcctg cagagtgagc tacaaggcta tctaagttcc
     4741 attttacacc tgtacctggt gaaacgaaat ttgcaaaaga gatatttgct atgcaaagaa
     4801 atattagtgt gtaaaaaaat tttttcatgc taattctatt taaagttgaa gttaatacag
     4861 tatagttttt aaaatgttta aacagcaaat ttacaaaagg aaaaataaaa tatgcaagat
     4921 ataaattcta cttttctatt taaccctaca acaaaaatgt cattgcttcc ttaaacaatg
     4981 acattttata ttcatgaact attatagtta aagcctttcg tatagttttc ttaattttta
     5041 ttttgtcata attgcttcct agtttttacc taaagtattc cattataatg gttttaagct
     5101 atgatttata actatcaatt ataaaaaaaa ataggcagaa attttaaaaa tctgcctaaa
     5161 acaatagtac taaatttatg aattaatgtt taataaattg catttgttct atccctttat
     5221 ctgtaagtag ttttaaggta taaacgcctc tacttataaa aggtgtttta atatttaatt
     5281 tttgatcact taaactaata ttgatagtag aggaatacac aatttggcca tttgcattta
     5341 atatttgtac taatgcttta cctgcaaaag gtttattaat atctatatta aacgattcca
     5401 ttacagggtt tggataaatt tttccaaatg ttttctctac tttttttgtg gatactagaa
     5461 ttgttttgca aataaactgg taactattgt ctttatccac ttgtttaatt ctataataat
     5521 aaaaagcatt tggttttatg gtataatctg tataagagta tttactgcca ttaatattat
     5581 tatttgaatt aaaagaagta ataggaacaa aattttctcc attctcgctt ctctcaattt
     5641 gataatagct attgttattt tcttgtaagg ttgagaagaa aagttgaacc gaattattat
     5701 atgttttata ataaaaattg ttgcaagttg atggaactaa aagattgtca gctgcatgtt
     5761 gtaaaaagaa tccagaaaaa ttaggagtaa tcatatctac ataataccct gttggagtgc
     5821 ttggtgttgt tgaattacta ataaaccc
//
LOCUS       DAOQPK010000143         5734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_8412_length_5734_cov_4.920409, whole genome shotgun sequence.
ACCESSION   DAOQPK010000143 DAOQPK010000000
VERSION     DAOQPK010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8412_length_5734_cov_4.920409"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..706
                     /locus_tag="PLU36_07460"
     CDS             116..706
                     /locus_tag="PLU36_07460"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HMZ46624.1"
                     /translation="MQYSYKDLEPYIDAATMEIHYTKHAAGYTKNLNEALKDEYKKSN
                     AITIEDILAKISSYSSKMRNNAGGHYNHELFWQTLTPKSSGKPTQKVLEVINNSFGSF
                     EEFKKLFSQAAATRFGSGWAWLIENDGHLKISSTPNQDNPLMDVAEVKGYPILGLDVW
                     EHAYYLKYQNKRTEYINNWWNIINWEAVEKRLLNKK"
     gene            751..1740
                     /locus_tag="PLU36_07465"
     CDS             751..1740
                     /locus_tag="PLU36_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HMZ46625.1"
                     /translation="MQQAKQNFRVQLFITILSIILFSVKMAAYYFTYSLVVLSDALES
                     IVNMVAGCIGLFALYVAAKPKDVNHPYGHGKAEFVSAAIEGTLIIASGLLIIYQTIVS
                     LFYPIQLNSLDKGLWLIAATAIINFIAGTICLQIGKKNNSIALKATGKHLYTDTLSTI
                     VVIAGLLIMLYSKMYWLDKVLAFAISFIILYNGYKIIRTSMAGIMDEADMELLKQIIK
                     VLNENRRENWIDLHNFRVIKYGRLLHIDCHLTIPWYLNIHQAHEEVDLLTNLIKEKFG
                     GGIELFVHTDGCLNFSCSICLKQNCIERKRPFLHKIEWTLENVLSNQKHNSVI"
     gene            complement(1733..2395)
                     /locus_tag="PLU36_07470"
     CDS             complement(1733..2395)
                     /locus_tag="PLU36_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46626.1"
                     /translation="MKKTLIAFIAFIAILSLNSCKKSSSGSTSTDTYLPNNVGTNWKY
                     KVTTGGASSDVTYTVSTNTLVVDGITYKIITGSNGNEQYYAKVGSDYHSLIKVSGQNV
                     HLIILKDDKNVNDTWTTTQTLTGLSIPGVPVSSATVNITYTLKEKGTTRVVEGVTYNN
                     VIKVEAALSASAAGFPLSLGTIEYYFSKNIGIIENTANINNSTIGVSINDKYELKTYE
                     IK"
     gene            2567..4243
                     /locus_tag="PLU36_07475"
     CDS             2567..4243
                     /locus_tag="PLU36_07475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HMZ46627.1"
                     /translation="MKLLAASFLVLISFNSIAQQKNSILITSQLSSANVFYGYGAELT
                     HKAKANIKKGQQEIVIKNISTTADENTLQIATPENVVLLSYKLEIKQEGIKPTNPLIK
                     KMEDSLKILQKQINANNQDWQIQDEQLTKTSKILETYTSSTGKNINTLEVLKLIDYYN
                     GKVQFFRNRMYAIKIKVNDLQEQVFDITNRLVELRKKEYAEAKKPIANLVLQVISKEA
                     MEADFAISYYTQNAGWVPTYDMRIKSIDNNFKLAYKALISQTTGIDWQQTKLVLSTSN
                     PNLGTTIPVLSAWNLQLYVPQVYTTMRNGNNAAKAYNRVQGMKAEEIAVSGDAEYSTN
                     NSTASEIVTSDISRYTTLNESQLFTSFEIELPYDIPSDGLNYSVAIKEEKIQATYKHY
                     AIPKLDKDAFLLAEINNWEALDLLPGDANIIMDNVYLGKSFIDPNSTLDTLNISLGRD
                     RRVSTKRMLMKEFSKTRFIGSNKIESFTYEVTVKNNKKEVVNMILKDQYPISRIKEVE
                     IKLEESSNAEINEELGTLNWKISLQPGEQKKYRFSYTVKYPKDKKIVNLR"
     gene            4336..5364
                     /gene="pheS"
                     /locus_tag="PLU36_07480"
     CDS             4336..5364
                     /gene="pheS"
                     /locus_tag="PLU36_07480"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HMZ46628.1"
                     /translation="MNSLVQQLDIYKQEIISYNVSNADEVEAFRIKYLGTKGIIKHAM
                     GFMKDVPNEQKREAGQLLNEFKLMVEAKYESLKESFSASEQITNTIDFSLPGNIYNVG
                     TRHPLSIIRNKIVSIFQRLGFAIAEGPEIEDDWHNFGAMNLPEDHPARDMQDTFYVNQ
                     NPTWLLRTHTSSVQARVMENQKPPIRVICPGRVYRNETISARAHCFFHQVEGLYIDEN
                     VSFADLKQTLYFFVQEMFGKDVKVRFRPSYFPFTEPSAEMDISCLICNGDGCNVCKHT
                     GWVEILGSGMVHPKVLENFGIDSNKYTGFAFGMGVERICQLKYRVNDLRLYSQNDIRF
                     LKQFTSVI"
     gene            5544..>5734
                     /locus_tag="PLU36_07485"
     CDS             5544..>5734
                     /locus_tag="PLU36_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HMZ46629.1"
                     /translation="MKCSAIQLLNEKETRAGFGEGVYEAGKENENVVVLTADLAGSMK
                     IAPFIKDFPNRFIQVGIAEA"
BASE COUNT         2171 a          794 c          925 g         1844 t
ORIGIN      
        1 aaatagaaga aattttatta caaatacagc aaaagttggt attggtttaa ccattcttcc
       61 aacaatatta caagccaaag aagatggtgt agtagtaaat tttaaacaac aacccttgca
      121 gtatagttat aaagatttgg aaccatatat tgatgctgct acaatggaaa ttcattatac
      181 aaaacacgct gcaggttata ccaaaaacct gaatgaggct ttgaaagatg aatacaagaa
      241 atctaatgca attactatag aagatatttt agctaaaatt tcttcttact cctctaaaat
      301 gagaaacaat gctggtgggc attataatca tgaattattt tggcaaacgc ttacgcctaa
      361 atcatcaggc aagccaacac aaaaagtact agaagtaatt aacaattctt ttggcagttt
      421 tgaggagttt aaaaaacttt tttcacaagc agcggctaca cgttttggta gcggttgggc
      481 atggttaatt gaaaacgacg gccatttaaa aattagctct acacccaatc aagataaccc
      541 attaatggat gttgcagaag tgaaaggata ccctatttta ggtttagatg tttgggagca
      601 tgcctattat ttaaaatacc aaaacaaacg tactgagtat attaataatt ggtggaatat
      661 tattaattgg gaggctgtag aaaaaaggct tttaaataaa aaatagtttt agctattata
      721 taaattgtta aaccaattaa ttacaaataa ttgcaacagg caaaacaaaa ttttcgtgta
      781 caattattta ttaccatttt aagtataata cttttttcgg ttaaaatggc tgcttattac
      841 tttacctatt cattggtagt attaagcgat gccttagaaa gtattgttaa tatggttgca
      901 ggttgtatag gtttatttgc tttatatgtt gcagcaaagc ctaaagatgt taatcaccct
      961 tatgggcatg gtaaagcaga gtttgtttct gccgctatag aaggtacttt aattatagcc
     1021 tctgggttat taattatata tcaaaccatt gtttctttat tttatccaat acagttaaac
     1081 agtttagata aaggcttatg gcttatagca gccacagcaa ttattaattt tattgcagga
     1141 actatttgtt tgcaaatagg taaaaaaaat aattcaattg cattaaaagc tacaggtaaa
     1201 catttatata ccgatacctt atcaaccatt gttgttattg caggcttatt aattatgctt
     1261 tactctaaaa tgtattggtt agataaggta ttagcatttg ctataagttt tatcatattg
     1321 tacaatggct ataaaattat acgaacatcc atggctggta ttatggatga ggcagatatg
     1381 gaacttttaa agcaaattat taaagtatta aatgaaaaca gaagagaaaa ctggatagat
     1441 cttcacaact ttagagtaat aaaatatgga agattactac atatagattg ccatttaact
     1501 attccatggt atcttaatat tcatcaggca cacgaagaag ttgatttatt aactaaccta
     1561 attaaagaaa aatttggagg cggaattgaa ctttttgttc atacagatgg ttgcttaaac
     1621 tttagttgta gtatttgttt aaaacaaaac tgtattgaaa gaaaacgtcc attcttacat
     1681 aaaatagaat ggacgttaga aaatgtttta tctaatcaaa aacacaatag tgttatttaa
     1741 tttcatacgt ttttaattcg tatttatcat ttatacttac accaatagtt gaattattaa
     1801 tattagcagt gttttctata atacctatgt ttttagaaaa ataatattca atggttccta
     1861 aacttaaagg aaaacctgca gcacttgcag acagtgcagc ttctacttta atcacattat
     1921 tataagttac accttctaca actcttgttg tacctttctc ttttaatgta taggtaatgt
     1981 ttacagttgc agaactaaca ggtacaccag gtatgcttaa accagtaagt gtttgggtgg
     2041 tggtccaggt atcgttaaca tttttatcat cttttaaaat aattaaatga acattttgac
     2101 cagaaacttt aattaaacta tgatagtcag agcctacttt agcatagtat tgttcgttgc
     2161 cattagagcc agtaataatt ttataagtta tcccatctac tactaaagta tttgtactta
     2221 cagtataagt aacatcagaa gatgctcctc cagtagtaac tttatacttc caatttgtac
     2281 ctacattatt aggtaaataa gtatcggtag atgtactacc actgctgctt tttttacaac
     2341 tgtttaaact taaaattgct ataaaagcaa taaatgcaat gagtgttttt ttcatttgtt
     2401 attaattagg gttgcaaatt atttatattt ttttataaat agaaatattt agaaacaagt
     2461 atacgaaaag tttcatactt tatgttaact tttcttttgc taaaaattta tgtatttatt
     2521 taaaaaatac catctattgg gtattattta ataaaataaa accattatga aattattagc
     2581 agcatctttt ttagtactta tcagttttaa tagtattgcc caacaaaaaa attccatttt
     2641 aattacctct caactatcca gtgcaaatgt attttatggt tatggggcag aattaacaca
     2701 taaagcaaaa gctaatatta aaaagggaca acaagaaatt gtaattaaaa acatcagcac
     2761 tactgcagat gaaaatactc ttcaaattgc cactccagaa aatgtagtat tactaagtta
     2821 taaacttgaa attaaacaag aaggaatcaa gcctaccaat cctttaatta aaaaaatgga
     2881 ggatagcctt aaaattctac aaaagcaaat aaatgcaaat aatcaagact ggcaaataca
     2941 agatgagcag cttactaaaa caagtaaaat tttagaaact tatacaagtt caacaggtaa
     3001 aaacataaac acactagaag tgctaaaatt aattgattat tataacggaa aagtgcaatt
     3061 ttttagaaac agaatgtatg caattaaaat aaaagtaaac gaccttcaag agcaagtttt
     3121 tgatataacc aataggctgg tagaacttag aaaaaaagag tatgcagagg caaaaaaacc
     3181 aattgcaaac ttggtgcttc aagttatatc taaagaagca atggaagctg attttgctat
     3241 ttcttattac actcaaaatg caggctgggt accaacttat gatatgagaa ttaaaagtat
     3301 agacaataat tttaagcttg catacaaagc attaattagc caaacaactg gtatagattg
     3361 gcagcagaca aaactagtat taagtacaag taatcctaat ttaggaacta ctattcctgt
     3421 attaagtgca tggaatttac agttatatgt accacaagtt tataccacaa tgcgaaatgg
     3481 caataatgct gcaaaagctt ataatagagt acaaggaatg aaagcagaag aaattgctgt
     3541 gagtggcgat gcagaatatt ctaccaataa ttcaactgca tccgaaattg taactagcga
     3601 tattagcagg tacactacat taaacgaaag tcaattattt acaagttttg aaatagagct
     3661 accttatgat atacctagcg atggattaaa ttacagtgtt gcaataaaag aagaaaaaat
     3721 tcaggcaacc tacaaacact atgcaatacc taaattagat aaagatgctt ttttgttggc
     3781 agaaataaat aattgggagg ctttagattt attaccaggc gatgctaaca taattatgga
     3841 caatgtttat ttaggcaaat cttttattga ccctaattct accttagata ctttaaatat
     3901 ttctttaggt agagatagaa gagtttcaac caaacgaatg ttaatgaaag agttttcaaa
     3961 aaccaggttt attggtagca ataaaataga aagttttact tatgaagtaa cagttaaaaa
     4021 taataaaaag gaagttgtga atatgatatt aaaagatcag tatccaataa gcagaataaa
     4081 agaagtggaa ataaaattag aggaaagcag taatgcagaa attaacgaag agttaggtac
     4141 tttaaactgg aaaataagct tacagcctgg cgaacaaaaa aaatataggt ttagttacac
     4201 agttaaatat cctaaagata aaaaaattgt taacttaaga tagctataaa aagcaacagg
     4261 ctataaaact atagcctgtt tttattttac aatttttcat actacaaata ggtttcatag
     4321 ccttatcttc gcccaatgaa tagcttggta caacaattag atatttataa acaagaaatt
     4381 attagttaca acgtatcaaa tgccgatgaa gtagaagcat ttagaattaa atatttgggc
     4441 acaaaaggca ttataaaaca tgcaatgggc tttatgaaag atgtacccaa tgaacaaaaa
     4501 agagaagctg gccagctttt aaatgaattt aaattaatgg tagaagcaaa gtacgaatca
     4561 ttaaaagaat ccttttctgc ttctgaacaa attactaata ctattgactt ttcattacct
     4621 ggaaatattt acaatgtagg aaccaggcat ccattaagta taataagaaa taaaatagtt
     4681 tccatatttc aaaggcttgg ttttgctata gccgaaggtc ctgaaattga agatgactgg
     4741 cacaactttg gagcaatgaa tttgccagaa gatcatcctg caagagacat gcaagatacc
     4801 ttttatgtaa accaaaatcc tacatggctt ttgagaacac atacaagtag tgtgcaagct
     4861 agagtaatgg aaaatcaaaa accacctatt cgtgttattt gccctggcag ggtttacaga
     4921 aatgaaacaa ttagtgcaag agcacattgt ttctttcatc aagtagaagg tttgtatatt
     4981 gatgaaaatg taagctttgc agatttaaag caaacactat atttctttgt acaagaaatg
     5041 tttggaaaag atgtaaaagt tagatttaga ccaagctatt ttccttttac agaaccaagt
     5101 gccgaaatgg atataagttg tttaatttgc aatggcgatg gatgtaatgt atgtaaacat
     5161 actggctggg ttgaaatttt aggaagtggt atggttcatc ctaaagttct agaaaatttt
     5221 ggaatagaca gcaataaata tactggtttt gcttttggta tgggagtaga aagaatttgc
     5281 cagttaaaat atcgtgtaaa cgatttaaga ttgtatagcc aaaatgatat taggttttta
     5341 aaacagttta ccagtgtaat ataagttaat gtttacggcg taagtttaat tctaattaaa
     5401 atctaaacaa attttaaaac ttacaagttg tataaaaatc ttttgaaata aattaaataa
     5461 ttgtacttct aatattttaa ctaagcactg tttttccttt acatttgcac cactattgtt
     5521 acccaatttt aaaatttttt gtaatgaaat gctcagcaat acaactacta aacgaaaaag
     5581 aaacacgtgc aggttttgga gaaggtgttt atgaggcagg taaagaaaac gaaaatgtag
     5641 tagtactcac tgcggatctt gcaggctcca tgaaaattgc accttttata aaagattttc
     5701 ccaatcgttt tatacaagtt ggtattgcag aagc
//
LOCUS       DAOQPK010000144         5716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_8460_length_5716_cov_3.247129, whole genome shotgun sequence.
ACCESSION   DAOQPK010000144 DAOQPK010000000
VERSION     DAOQPK010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8460_length_5716_cov_3.247129"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="PLU36_07490"
     CDS             complement(<1..807)
                     /locus_tag="PLU36_07490"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HMZ46630.1"
                     /translation="MLFQLQPFSPLVTATVNLLQQLQVKRITPNTVNEILQNHPDYPS
                     LLSINDSLQQWHIDTAAFMAEPEKLHELPAPFIAVLNNKDFVTVIQNSETITYKNGTN
                     KKAITKTKEDFIKEWKGIVLLATATEQSGDANYVKEKQKELLQAAKLPLVILLLAAIG
                     LFAMYKSNWHIGFSLFLLIKLAGTVVVSLLLWYEIDKTNPLLKQICSATKTTNCSAIL
                     ESKQSKLLGLLSWSEIGFIYFAGGFLFLLFAAATKNIQPALSLIAWLNVLA"
     gene            1072..1632
                     /locus_tag="PLU36_07495"
     CDS             1072..1632
                     /locus_tag="PLU36_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HMZ46631.1"
                     /translation="MSIILASQSPRRKQLLEWAEIDFEVMVVPTDESYPDYLAIEEIP
                     IHIARQKAFAVEQVIGKEKMILAADTLVVLHENIIGKPKDREDAIKILSNLSGNVHSV
                     ITGVVLLNGFKEIAFAVVTEVTFHTLTQEQIIFYVDKYKPYDKAGAYAIQEWIGVVGI
                     QSIKGDFYNVMGLPVSRVVQVIKDMM"
     gene            1718..2989
                     /locus_tag="PLU36_07500"
     CDS             1718..2989
                     /locus_tag="PLU36_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HMZ46632.1"
                     /translation="MTEKLLHFIWQFQYFNKTALQTEEGEELIILNQGTLNTNQGPDF
                     LLASVKINNITFVGNIELHINASDWYKHKHSNDENYNNVILHVVWNYDKPLVVINKII
                     PTLELKTLVAKLLLQRYKSLMEFTTKIPCQTFLPALTTIGWMSWKERLAAERLEIKAT
                     EILQLFQESKQHWEETFWWLLAANFGLKINQQVFKEIAQTIPVQVLAKHKNQMQQLEA
                     LLFGQANFLNEKPTDEYTTLLQKEFQFLQHKYKLKKVNAKVLFLRMRPASFPTIRLAQ
                     LAMLIHNSSHLFSKIKELKTVEEVKLLFDITANDYWHYHYNFSEKSAYQPKSLGNQMI
                     ENIIINTVVPMLFAYGLYNKEEHYKTKAIEWLQALSPEKNSVITEWKNTEITAQTAFD
                     TQALLQLNKFYCTKLNCLSCAVGVKILRASN"
     gene            3072..4367
                     /locus_tag="PLU36_07505"
     CDS             3072..4367
                     /locus_tag="PLU36_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HMZ46633.1"
                     /translation="MKYLPLNPEIFIHNRKRFVKEMAPNSIAIIVSNDEFPSNGDALH
                     SFKQNSDLYWLTGIEQEDSLIILFPDNPDPKYREVLVLVRPNELKEKWDGKRLRANEG
                     TSISGIQTIVWLDSLDALMQAWIHLADNIYLDSNENDRKANLIRSREYRFIDEMKARY
                     PLHHYKRAAQIFGKLRAIKSKEEVEVIQHAINITDNTFRRLLQFIQPGVFEYEIEAEL
                     IHSFLSQRATGEAYGSILASGDRARTLHYVSNNQECRDGELILMDFGAAYGGYCADLT
                     RTVPVNGKFTKRQKIVYNACLHLHNYAKSILKPGISIVDYTDKVGEEATQQFLKIGLL
                     KKMDIKNEDKDNRAYRKYLYHGISHHLGIDVHDLGTRTAPIQAGMVFTVEPGIYIEEE
                     QMGIRIENNLWITKNGHIDLMKNIPITVEEIEALMKKQK"
     gene            4391..5152
                     /gene="pssA"
                     /locus_tag="PLU36_07510"
     CDS             4391..5152
                     /gene="pssA"
                     /locus_tag="PLU36_07510"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HMZ46634.1"
                     /translation="MIKQIPNLFTLLNLFFGCLAVVTSLQSGLTIVLDGYGEQLVEIP
                     ESIFMASIFIGIAAIIDFLDGFVARLFKATSEIGKQLDSLADVVSFGVAPSIIVYQFL
                     RLSFAQQEGGLDVNTLWLMPAFIIPCCGAYRLARYNLDTQETSSFKGVPIPAVGLLIA
                     SYPIVYWYSGNANYLINLLRNQWFWYAIIILVSYLMVSTLPMLSLKFKGVSFKNLLPI
                     ILILLISFIAAFFVGWFAVTIGFITYVIVSLLFKN"
     gene            5178..5426
                     /gene="purS"
                     /locus_tag="PLU36_07515"
     CDS             5178..5426
                     /gene="purS"
                     /locus_tag="PLU36_07515"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HMZ46635.1"
                     /translation="MLYNIQIKVMPLKDLLDPQGKAIQNGMKNLGITSVTDVRVGKHV
                     TLQIDAANENEAKLIAEEAGKKLLANPVMEFFEIELVN"
     gene            5439..>5716
                     /locus_tag="PLU36_07520"
     CDS             5439..>5716
                     /locus_tag="PLU36_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HMZ46636.1"
                     /translation="MLYLVPTPIGNLKDFTYRAVEILKGVDVILCEDTRTSSRLLDYY
                     EINKPLSPYHKFNEHKIVAHLVQQMQAGKVIALITDAGTPGISDPAFL"
BASE COUNT         2058 a          806 c          948 g         1904 t
ORIGIN      
        1 tgccaataca ttcagccatg ctattaaaga taaagcaggt tgtatgtttt tagtagctgc
       61 cgcaaaaagt aaaaataaaa aaccgcctgc aaaatatata aagcctattt cgctccaact
      121 tagtaaaccc aataatttag attgcttact ttcaagtata gcactgcaat tggtagtttt
      181 agtagcagag catatttgtt ttaataaagg atttgttttg tctatctcat accataataa
      241 cagggaaaca actacagtac ctgccaactt tattaaaaga aataaagaaa acccaatatg
      301 ccaattactt ttgtacatgg caaataagcc aatggctgca agcagtaata ttaccaacgg
      361 tagtttggca gcctgcaaca attctttttg tttttctttt acataattag catctcccga
      421 ttgctcggta gctgttgcta ataatacaat gcctttccat tcttttataa aatcttcttt
      481 tgttttggta atggcttttt tgttagtgcc gtttttataa gtaatagttt cgctgttttg
      541 tattacagtt acaaaatctt tgttgtttaa cacagcaata aaaggtgcag gtaattcatg
      601 tagtttttca ggctctgcca taaaagcggc agtatctata tgccattgct gtaagctatc
      661 gttaatactt aaaagacttg gataatcggg gtgattttgt aagatttcat ttactgtatt
      721 tggcgtaata cgttttacct gcaattgttg taataaatta acagtcgcag taacaagagg
      781 gctaaagggt tgcagttgaa aaagcataat attagaattt ggtattagct tttgaattta
      841 tatgaagata aaagtataaa atcagatttc taaaaatgcc ccccccccct tattaacaaa
      901 aagttaacat attttaatga taataaatta catggagtat taagtgtatt ttattgtatt
      961 ttttacattt tcaaagatta ttaacctcaa cagaatataa aaataaatac tgttttaagt
     1021 atttactttt acttactaaa ttattttcaa aagaaaaata aataacattt tatgtcaatc
     1081 attcttgcat cacaatctcc aagaagaaaa caattattag aatgggcaga aattgatttt
     1141 gaagtaatgg ttgtgcctac agacgaatct tatcctgatt accttgctat tgaagaaatt
     1201 ccaattcata ttgccagaca aaaagctttt gcagtagagc aagtaattgg taaagaaaaa
     1261 atgattcttg cagccgatac tcttgtagtt ttacatgaaa acattattgg aaaacctaag
     1321 gatagagaag atgccataaa aattctttcc aacttatcag gtaatgttca tagcgtaata
     1381 acaggtgtgg ttttattgaa tgggtttaaa gaaatagcat ttgccgttgt tacagaagta
     1441 acttttcata cacttacaca agagcagata attttttatg tggacaaata caagccttat
     1501 gataaagcag gagcttatgc catacaggaa tggattggtg tggtaggcat acaatccatt
     1561 aaaggcgatt tttataatgt aatgggatta cctgtgagta gggtagtgca ggttataaaa
     1621 gatatgatgt gaaagtgatt gtttaatgaa gcgggataat agtttttaat ttctctataa
     1681 aaaatatttt gtatcttttg tcttacaatt tatttgtatg accgaaaagc tgttacattt
     1741 tatttggcaa tttcaatatt ttaataaaac tgctttacaa acagaagaag gcgaggaatt
     1801 gattatttta aatcaaggta cacttaatac caatcaagga cctgattttt tattagcttc
     1861 tgtaaaaatt aataacatca cttttgttgg caatattgag ttgcatatca atgcatcgga
     1921 ttggtataaa cacaaacaca gcaatgatga gaattacaac aatgtaatat tgcatgtagt
     1981 ttggaattac gataaaccat tggttgtaat aaataaaatt attccaacct tagaactgaa
     2041 aactttagtg gcaaaactgt tattgcaacg ctacaaaagt ttaatggaat ttacaacaaa
     2101 aattccttgc caaacatttt tacctgcttt aacaactatt ggctggatgt cttggaaaga
     2161 aaggcttgca gccgaaagat tagaaattaa agcaacagaa attttacaat tatttcaaga
     2221 aagtaaacaa cattgggagg aaactttttg gtggttgttg gctgctaatt ttggattaaa
     2281 aattaaccaa caagttttta aagaaattgc acaaactatt cctgtacaag ttttagcaaa
     2341 acacaaaaat caaatgcagc agttggaggc tttgttattt ggtcaggcta attttttaaa
     2401 tgaaaaacca acagatgaat acactacgtt attgcaaaaa gaatttcagt ttttacaaca
     2461 caaatacaaa ttaaaaaaag taaatgctaa ggttttgttt ttaagaatga ggcctgcctc
     2521 ttttcctacc attcgtttgg ctcaattagc tatgttgata cacaacagca gtcatttatt
     2581 ttcaaaaata aaagagttga aaactgtaga agaggtaaag cttttatttg atattacagc
     2641 caatgattat tggcattatc attacaattt tagtgaaaaa tcagcttatc aacccaaaag
     2701 cttaggcaac caaatgatag aaaatattat tattaatact gttgtaccaa tgcttttcgc
     2761 ttatggtttg tataataagg aagaacatta caaaaccaaa gctatagaat ggttacaagc
     2821 gttatcgcct gagaaaaatt ctgtaattac cgagtggaaa aatactgaaa ttaccgccca
     2881 aactgcattt gatacacaag ctctattaca attaaataaa ttttactgca ccaaattaaa
     2941 ttgtttgagt tgtgctgttg gtgtaaaaat tttaagagct agcaattaaa aaaaatctgt
     3001 taccctttag cagttttatt ttatttgctc tgtataaatt gttatatctt gcaaacttta
     3061 aaatttttga tatgaaatat ctaccattga acccagagat ttttattcat aatcgtaagc
     3121 gttttgtaaa agaaatggca cccaacagta tagccattat tgtaagtaac gatgaatttc
     3181 ctagcaatgg cgatgctctt cattctttta aacaaaacag cgatttatat tggcttactg
     3241 gcattgaaca agaagattcc ttgattatac tttttccaga taatccagat ccaaaatata
     3301 gagaagtatt ggttttagtt cgtcctaatg aattaaaaga aaaatgggat ggtaaaagat
     3361 taagagccaa cgaaggtact tccatttctg gcatacaaac tatagtatgg ttagatagcc
     3421 tggatgcttt aatgcaagct tggatacatt tagcagataa tatttattta gatagcaatg
     3481 aaaatgatag aaaagctaac cttatacgta gcagagaata tagatttatt gatgaaatga
     3541 aggctcgtta ccctttacac cattataaaa gagcagccca aatttttgga aaacttagag
     3601 caattaaatc taaagaagaa gttgaagtaa tacaacatgc cataaatatt acagataata
     3661 cttttagaag acttttacaa tttatacaac caggtgtttt tgaatatgaa attgaagcag
     3721 aattaataca tagcttttta agccaacgag ctacaggaga agcctatggt agcattttag
     3781 ccagtggaga cagggcaaga accttacatt atgtaagcaa taaccaagaa tgtagagacg
     3841 gagaattaat attgatggac tttggtgctg cttatggtgg ctattgtgca gatttaacca
     3901 gaactgtacc ggtaaacgga aaatttacca aacgccaaaa aatagtttat aatgcttgtt
     3961 tacatttaca caattatgct aaaagcattt taaaacctgg aatatctatt gttgattata
     4021 ccgataaagt tggcgaagaa gcaacacagc aatttttaaa aataggacta cttaaaaaga
     4081 tggatataaa aaatgaagac aaagacaata gagcctatcg taaatattta taccatggta
     4141 taagccatca tttaggaatt gatgtgcatg atttaggtac acgaactgct ccaatacaag
     4201 caggcatggt ttttactgta gagccaggca tttatattga agaagaacaa atgggaatta
     4261 gaattgaaaa caacttatgg ataacaaaaa acggacatat tgatttaatg aaaaatattc
     4321 cgattactgt tgaagaaatt gaggctttaa tgaaaaaaca aaaataatat tagcaaaata
     4381 ttcttattca atgataaaac aaattcccaa tttatttact ctattaaatt tattttttgg
     4441 ttgtttagcc gttgtaacct ctttacaaag tggtttaact attgtgttag atggatatgg
     4501 tgaacaatta gtagaaatac ctgaaagcat ttttatggca agtattttta ttggcattgc
     4561 agcaataatt gattttttag atggttttgt tgcaaggttg tttaaagcaa cttccgaaat
     4621 aggaaagcaa ttagatagtt tagcagatgt agtaagtttt ggtgttgcac cttccattat
     4681 tgtatatcag tttttacgtt tatcatttgc acaacaagaa ggtggtttag atgttaatac
     4741 tttatggctt atgcctgctt ttatcatacc atgttgtggt gcatatagat tagccagata
     4801 taatttagat acccaagaaa cttcaagttt taaaggtgtt ccaataccag cagttggttt
     4861 acttattgca tcttatccaa ttgtatattg gtatagtggc aatgcaaatt atttaataaa
     4921 cttattaaga aatcagtggt tttggtatgc tatcataatt ttagtgagct atctaatggt
     4981 aagtacatta cccatgctct ctttaaaatt taaaggagta agctttaaaa atcttcttcc
     5041 aattatactt attttattaa taagttttat tgcagcattt tttgttggct ggtttgcagt
     5101 taccataggt tttattactt atgtaattgt atctttactt tttaaaaatt aatatttaca
     5161 tacaaaaaaa aaatcaaatg ttgtataata ttcaaataaa agtaatgcct ttaaaagatt
     5221 tattagaccc acaaggaaaa gctattcaaa atgggatgaa gaatttaggt attaccagtg
     5281 ttacagatgt aagagtaggt aaacatgtaa cattacaaat tgatgctgcc aatgaaaacg
     5341 aagcaaagtt aattgctgaa gaagcaggaa aaaaactatt ggcaaaccca gtaatggaat
     5401 tttttgaaat tgaacttgta aattaatgct aatataaaat gctttacctt gtaccaacac
     5461 caataggtaa cttaaaagat tttacttacc gtgctgttga aattttaaaa ggtgttgatg
     5521 taatattatg tgaagataca agaacctcat ctcggctttt agattattac gaaattaata
     5581 aaccactttc tccctatcat aaatttaacg aacataaaat tgttgctcat ttagtacagc
     5641 aaatgcaagc tggtaaagta attgccttaa ttacagatgc tggcacacca ggtattagcg
     5701 acccagcatt tttatt
//
LOCUS       DAOQPK010000145         5616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_8700_length_5616_cov_4.198346, whole genome shotgun sequence.
ACCESSION   DAOQPK010000145 DAOQPK010000000
VERSION     DAOQPK010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8700_length_5616_cov_4.198346"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PLU36_07525"
     CDS             <1..373
                     /locus_tag="PLU36_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HMZ46637.1"
                     /translation="FPLFYGGPVQPQTIHFIHKRPDLIEGGLEVLENIYWGGEFELVV
                     DLIKTKKISKNDIRFFIGYSGWGEGQLADELQTKSWITRQATTALVFNSKTENIWKDA
                     LKDLGGEYSQMVNYPIDPQLN"
     gene            509..1447
                     /locus_tag="PLU36_07530"
     CDS             509..1447
                     /locus_tag="PLU36_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ46638.1"
                     /translation="MDKILELNNLQKFFSSQKAVDDISFSIDKGSIFGLLGPNGAGKT
                     TLIRMITGIFYPDNGSIIFDGKKFDPINDVLKIGYMPEERGLYKKMKIGEQALYLAQL
                     KGMSKKDAMERIIFWFKKLEMESWWNKKVEDLSKGMSQKLQFVTTVLHEPKLIILDEP
                     FSGLDPINANLIKDEIYGLAQRGSTVIFSTHRMEQVEEICDHIVLVNLGKKILDGTVQ
                     DIKQQFKKNKFSITLQNEPTNTLSNVFTIDAQNNNSLTVSINNGYKSNDVLKHFIDTN
                     QAIESFNEILPGLNEIFISLVEGTKAKTRAFEAVTV"
     gene            1479..2822
                     /locus_tag="PLU36_07535"
     CDS             1479..2822
                     /locus_tag="PLU36_07535"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ46639.1"
                     /translation="MNKILIIARREFLSRVQKKTFLLTTIGLPILIFGIYALIIYFAV
                     KTTDNFTIAIADEANIFEGKLAKKNDGEINFIHVQNETPETLKKQIEAKKINAFVFIP
                     KTFDITGSTDSIQVISDKSVGLITREKIIGKLNNVIEEKKMLGSLHITKTQLDSLQKT
                     NEIVFTTTKGVEDTSKKAGISYGVGFVSGFLIYIILFIYGTMVMRGVMEEKTNRIAEV
                     IVSSVKPFQLMMGKIFGIGSVGLVQFLIWIILIIGLQFLLPIIFPDMLTQMQAQPMQP
                     GIGAAASTMSQENTAMVGIMNGIGQVNWLLIVGCFLFYFLGGYFLYSSLFAAVGSTVN
                     EDPQDAQSLMLPITMPIIFGMVIMMKAVNDPNSSLAIFGSIFPLTSPIVMMARIAHGV
                     PEGVSILQLLISMACLIVGFVATTWLAGKIYRTGILMYGKKVNWKTIGSWILRKN"
     gene            complement(2899..3993)
                     /locus_tag="PLU36_07540"
     CDS             complement(2899..3993)
                     /locus_tag="PLU36_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HMZ46640.1"
                     /translation="MTVEAILDALRNVQEPDLGKDIVTLNMVKDIAIDGDKVSFTIVL
                     TTPACPMKDKMRMASENAVKLLVNKDAKVIVNFTAITSSKRLQGDKILGGVKNIIAVV
                     SGKGGVGKSTVSANLALALSSAGATVGLMDADIYGPSVPIMFGVRGQRPMMKEVDGKG
                     LIVPLEKYGIKLISIGLLVDEKNAVVWRGPMVSNAIRQFVTDVDWGELDYLVIDMPPG
                     TGDIHLTLMQTVPVTGVVIVSTPQKVALADAKKGIAMFGQSQINVPILGLVENMAYFT
                     PAELPNNKYYLFGKEGGKNLADEYDLNFLGQIPLVQSIMEGGDEGVPAMAGNDEVSKE
                     AFRNLTASIVRNIAIRNANIPATKIVEVKA"
     gene            4156..4812
                     /locus_tag="PLU36_07545"
     CDS             4156..4812
                     /locus_tag="PLU36_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMZ46641.1"
                     /translation="MMKLIVHKLFFAIVIGLFCTKISTAQRYVSQDSVVQLYGVIMSA
                     DSLRAISNASIIVVNKGRGTISNSDGVFSIAVLKGDRITFSCIGFKDKTIDIPHNLKE
                     NQYSVIQLLVNDTAYLPATIIRSRPSREQFERDFVNTPIPDDAYEIARQNTDVAKRRV
                     LINSLPADGREAVNYQMRQQANKYYYAGQIPPMNILNPMAWAEFIKSWKRGDYKRKKN
                     "
     gene            4876..>5616
                     /locus_tag="PLU36_07550"
     CDS             4876..>5616
                     /locus_tag="PLU36_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HMZ46642.1"
                     /translation="MKNISVIGAGTMGNGIAHVFAQNGFTVNLIDVNAAQLEKAIATI
                     TKNMDRQIAKGSFTEEQKTTALQNLTTYISIEEGVANAELVVEAATENIELKLKIFKQ
                     IDAAAPVGCILASNTSSISITKIASATKRPHLVIGMHFMNPVPVMKLVEIIEGYATEK
                     QVTESIVALSKQLGKIPCVVNDYPGFIANKILMPMINEAIISLYEGIAGVEEIDTIMK
                     LGMAHPMGPLQLADFIGLDVCLSILNVLY"
BASE COUNT         2010 a          836 c          923 g         1847 t
ORIGIN      
        1 cttcccttta ttttatggtg gtcccgttca gccacaaacc attcacttta ttcataaacg
       61 acctgattta attgaaggag gattagaggt attagagaat atttattggg gtggtgaatt
      121 tgaattagta gttgacttaa ttaaaacaaa aaaaattagc aaaaacgata ttcgtttttt
      181 tattggttat agtggctggg gcgaagggca attagcagat gaattacaaa caaaaagctg
      241 gataaccaga caagcaacca cagctttagt ttttaattca aaaacagaaa atatctggaa
      301 agatgcactg aaagatttag gaggtgagta cagtcaaatg gttaattatc caatagaccc
      361 acagttgaat taattcaatt tatactcaca ttttagtaat tcaactatta ataaatttcc
      421 tttacttctt ccattttttt cgccttttgt cattaataat ttaataaact actttcaaag
      481 atttactttt gaaaataaat atgtattcat ggataaaatt ttggaattaa ataatcttca
      541 aaagtttttt tcatcacaaa aagcagttga tgatattagc tttagtatag ataaaggaag
      601 catttttggt ttattaggcc ctaatggtgc aggcaaaacc actttaataa gaatgataac
      661 aggaattttt tatcctgata atggttctat aatatttgat ggaaaaaagt ttgacccaat
      721 taatgatgtt ttgaaaattg gctatatgcc agaagaaaga ggattgtaca agaaaatgaa
      781 aattggcgaa caagcactat acctggctca actaaaagga atgagcaaaa aagatgcaat
      841 ggagagaatt attttttggt ttaagaaatt agaaatggaa agttggtgga ataaaaaagt
      901 ggaagactta agtaaaggaa tgagccagaa attacaattt gtaaccactg ttttacatga
      961 acctaaatta ataatcttag atgaaccttt tagtggatta gaccctatta atgctaactt
     1021 aattaaagac gaaatttatg gcttagcaca aagaggaagt acagtaattt ttagcactca
     1081 ccgcatggag caagtagaag aaatttgcga tcatattgtt ttagtaaact taggaaagaa
     1141 aatattagat ggtacagttc aagatataaa acaacaattc aagaaaaata aatttagcat
     1201 tacactgcaa aacgagccta ccaatacttt aagcaacgta tttactatag atgcacaaaa
     1261 taataactct ctcacagttt caattaataa tggttataaa agtaacgatg tattaaagca
     1321 ttttattgat actaatcaag ccatagagtc tttcaacgaa atattaccag gtttaaatga
     1381 aatatttatt tcgttagtag aaggaaccaa agcaaaaaca agagcttttg aagccgtaac
     1441 agtttaattt ttatcagaac aatttatcta aataaaccat gaacaaaata ttaattatag
     1501 caagaagaga atttttaagt agagttcaaa aaaagacttt tttgcttaca acaatagggt
     1561 taccaatttt aatatttggc atttatgcat taataatata tttcgctgta aaaacaaccg
     1621 acaattttac cattgccatt gcagatgaag ccaatatttt tgaaggaaag ctagccaaga
     1681 aaaatgatgg agaaatcaat ttcattcatg tacaaaatga aacaccagaa actttaaaaa
     1741 aacaaattga agcaaaaaaa attaatgctt ttgtatttat tccaaaaaca tttgatatca
     1801 ctggttctac agattcaatt caagtaatat ccgacaaaag tgttggttta attacccgag
     1861 aaaaaattat cggtaagtta aataatgtaa ttgaagaaaa gaaaatgttg ggtagcttgc
     1921 atattaccaa aacacagcta gatagtttgc aaaaaacaaa tgaaattgtt tttacaacaa
     1981 ctaaaggggt ggaagatacc agtaaaaaag caggtatcag ttatggagta ggatttgttt
     2041 ctggctttct tatctacatt attcttttta tttatggcac tatggtaatg cgtggtgtta
     2101 tggaagaaaa aacaaataga attgcagaag taattgtaag tagtgtaaaa ccttttcaat
     2161 taatgatggg taaaatattt ggaattggct cagtagggct agttcaattt ttaatttgga
     2221 taattttaat tattggctta caatttttat tacccattat ttttcctgat atgttaactc
     2281 aaatgcaagc acagcccatg caaccaggta ttggagctgc agcttcaaca atgtctcaag
     2341 aaaatacagc aatggttggt attatgaatg gaattgggca agtaaattgg ttgctaattg
     2401 ttggctgttt tttattttat tttttaggag gatattttct gtattcttct ttgtttgcag
     2461 cagtaggcag cacagttaat gaagacccac aagatgctca aagtttaatg ttacccatta
     2521 ctatgcctat tatttttgga atggtaataa tgatgaaagc tgtaaacgac cctaacagca
     2581 gcttagctat ttttggtagc atttttcctt taacttctcc tattgttatg atggcaagaa
     2641 ttgcacatgg tgtaccagaa ggtgtatcta tattacagct acttataagc atggcttgtt
     2701 taattgttgg ttttgttgcc acaacttggt tagcaggtaa aatttataga acaggtattt
     2761 taatgtatgg gaaaaaagta aactggaaaa ctattggtag ttggatttta agaaaaaatt
     2821 aaagcaagtt cagtaaaaat aaaagtggct acattcaata taaaatatag ccacttttat
     2881 tttatagagg tgttaatact atgccttcac ctcaactatt tttgtagcag gaatatttgc
     2941 atttctaatt gctatgtttc taacaatgga ggcagttaaa tttctaaatg cctctttaga
     3001 aacttcatca ttaccagcca ttgctggcac accttcatcg ccaccttcca ttatagattg
     3061 tactaatgga atttgcccta aaaaattcaa atcatactca tccgccaaat tctttccacc
     3121 ttcttttcca aataaataat atttattatt tggtaattct gcaggagtaa aataggccat
     3181 attttctacc aaccccaaaa taggtacatt aatttgcgat tggccaaaca tggcaattcc
     3241 ttttttggca tctgctaaag caactttttg tggtgttgaa actataacaa cgccagtaac
     3301 aggcactgtt tgcataaggg ttaaatgaat atcacctgta ccaggtggca tatcaattac
     3361 caaataatcc aattctcccc aatctacatc tgttacaaat tgtcttatag cattgcttac
     3421 cattggccct ctccaaacaa ctgcattttt ttcatcaacc aataaaccaa tacttattaa
     3481 tttaattcca tacttttcca atggtacaat taaaccttta ccatctactt ctttcatcat
     3541 ggggcgttgc cctctaacac caaacattat tggtacactt ggcccataaa tatcagcatc
     3601 cattaaacca acagttgcac ctgcacttga caaagcaagg gctaaatttg cacttactgt
     3661 acttttacca acaccacctt taccacttac cactgcaata atatttttaa ccccacctaa
     3721 aatcttatct ccttgtaacc tttttgatga agttattgca gtgaaattaa caatcacttt
     3781 ggcatcttta tttaccaaaa gttttactgc attttcgctg gccattctca ttttatcttt
     3841 catggggcaa gcaggtgtag taagtacaat ggtaaaagaa actttatctc catctattgc
     3901 aatatctttt accatattta aggttactat atcttttcct aaatctggtt cttgcacatt
     3961 tcttaaggca tctaaaattg cttctacagt cattattgtt gaaagttttt gttaaaaaat
     4021 tatataagtt gcgaagataa aactttcaga ggtttacttt gttgaagttt acattttgaa
     4081 gttttgaatc aagaaatatt ttaaaattat ttttatttga aacttattat acattagttt
     4141 attttggtta attgaatgat gaaattaatt gtacataaat tattttttgc tattgtgata
     4201 gggttgtttt gcactaaaat ctctactgca caacgatatg tatcacaaga tagtgtggta
     4261 caattgtatg gtgtaatcat gtcagcagat agtttaagag caattagtaa tgcaagtatt
     4321 attgtagtta ataaaggaag aggaactatt agtaatagtg atggcgtatt tagtattgca
     4381 gttttaaaag gcgatagaat aacattttct tgtattgggt ttaaagataa aaccattgat
     4441 ataccacaca atttaaaaga aaaccaatat agtgttattc aattactagt aaacgataca
     4501 gcttatttac ctgctacaat tatacgttct aggccaagca gagaacagtt tgaaagagat
     4561 tttgtaaata caccaatacc agatgatgct tatgaaattg caaggcaaaa tacagatgtt
     4621 gctaaaagaa gagtattaat aaatagcttg cccgccgatg gaagagaagc tgtaaattat
     4681 caaatgaggc aacaagcaaa taaatattat tatgctggtc aaataccccc aatgaatatt
     4741 ttaaatccta tggcttgggc cgagtttatt aaaagctgga aacggggcga ttacaagcga
     4801 aagaaaaatt aatctgctta attaaaattc atctatccaa atttatttta ctttcgctgc
     4861 ctaatacttt tatacatgaa aaatatttct gtaattggtg ctggaacaat gggtaatggc
     4921 attgctcacg tttttgcaca aaatggtttt actgtaaacc ttattgacgt aaatgcagca
     4981 caattagaaa aagctattgc taccataaca aaaaatatgg acagacaaat agcaaaaggt
     5041 agctttaccg aagagcaaaa aacaactgct ttacaaaact taaccactta tataagtata
     5101 gaagaaggag tagcaaatgc agaattagtt gttgaggctg caacagaaaa tatagaatta
     5161 aaattaaaaa tatttaaaca aatagatgct gcagcaccag ttggttgtat tttagccagt
     5221 aacacttctt ctatttctat tacaaagatt gcctcggcaa ctaaaagacc acatttagtt
     5281 attggtatgc attttatgaa cccagttcct gtaatgaaat tagtagagat tattgaaggc
     5341 tatgcaacgg aaaagcaggt aacagaaagt attgttgcgt taagtaaaca attgggtaaa
     5401 ataccatgtg ttgtaaatga ttatccaggg tttattgcaa acaaaatatt aatgcctatg
     5461 attaatgagg caattattag tttatacgaa ggtattgcag gtgtggaaga aattgatact
     5521 attatgaagt taggaatggc acaccctatg ggacctttac aattagccga ctttattggt
     5581 ttagatgtat gcttaagcat tttaaacgta ttgtat
//
LOCUS       DAOQPK010000146         5614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_8703_length_5614_cov_5.284584, whole genome shotgun sequence.
ACCESSION   DAOQPK010000146 DAOQPK010000000
VERSION     DAOQPK010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8703_length_5614_cov_5.284584"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="PLU36_07555"
     CDS             <1..222
                     /locus_tag="PLU36_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46643.1"
                     /translation="NGMQRANYATEHDAFVAKKLAYVMCGGDLSEPQFVTEQYLLDLE
                     REAFLSLCGEKKTLERIQGVLKNGKPVRN"
     gene            532..1146
                     /locus_tag="PLU36_07560"
     CDS             532..1146
                     /locus_tag="PLU36_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46644.1"
                     /translation="MHFVPNNLYHIYNQGNNKQVIFHDEDDYYTFLRLTRNLFHSVCD
                     IVAYCCMPNHFHFIISTNGYSCEIKQQGNISIDAITNSIRKLLSSFARLYNKKYLQSG
                     SVFRQKTKSKCLTNCDIEVLTSMNGREYLFTCFHYVHQNPLRAGLVNKLEDWDFSSFK
                     DYAGLSSGTLINKEVAKFYDIYNPSNFIKESYMVISDEYIKFIV"
     gene            complement(1315..1476)
                     /locus_tag="PLU36_07565"
     CDS             complement(1315..1476)
                     /locus_tag="PLU36_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46645.1"
                     /translation="MKDSVTVKPFSSKEIIISLTPTSNIIYVEEEKPKSISIFSMDGK
                     EQTEKFVMN"
     gene            1684..2400
                     /locus_tag="PLU36_07570"
     CDS             1684..2400
                     /locus_tag="PLU36_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HMZ46646.1"
                     /translation="MICKKEWQQFFSNLTGYIIIAVFLIVNGLVLFVFDDNIFSFGYA
                     TLEKFFMLAPWVLLLLIPAVTMRSLPDEYKLGTIEVLKTLPLTPWQIITGKYFGNLLV
                     IFLSLLPTFIYIFSIQNLADGNGLDLAATIGSYIGLFFLAAVFTAIGICAGSFTNNAV
                     VAFIISLFACALLYYGFNAISKLSQLSGGIDYYLEMAGIDFHYKNISKGVIDTRDVVY
                     FLSIIFLLLLITVRNLKHKI"
     gene            2433..4136
                     /gene="gldG"
                     /locus_tag="PLU36_07575"
     CDS             2433..4136
                     /gene="gldG"
                     /locus_tag="PLU36_07575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HMZ46647.1"
                     /translation="MQKLLQHKFSWLLLIAAFAIVNFMASIVHTKIDLTKEKRYTISK
                     ATKDILQHIDDKLEITVFLEGNLPAGFQKLANTTNDFLQLLKEKNNAKITFQFISPDT
                     EVDGTNIKFEDTLVAMGATPINLTIQVKQGQESKVVFPYALIKYKTHTQLISLYSGNS
                     RMISQDEINNAEAMMEYKFLKAFDAFVFPEKPLVAYSFANGEPTDIRTYDLLQTLQKD
                     YSLYTFDIAQRPAIPDTFKVLMIVKPSLQFSEDEKLKIDQYLMRGGKILCFIDNLNAE
                     QDSLNFKPELVAYDRNLNLTDLLFKYGVRINTDLVMDLQCNFMPFAVGGAKENPQYEF
                     LHWNYYPLFESKNNHTINKNLGLVSGRFVNSIDTVKAEGITKTILLSSSDNSRKLGTP
                     ALISLNENRNTPQDILFQQKNIPVAVLLEGSFTSLYKNRLAQHIKDSVEKIGYPFLEK
                     SFSESKIIVVADGDIVLNDASQKLGPLPMGLNMYTVGTQYEYQFANREFLLNSLEYLT
                     NKQSIIQSRNKEQILRLLNTSKVEEEKVKWQMINIGLPLLFVIIYGWVYNLIRKKKFT
                     T"
     gene            4152..5111
                     /locus_tag="PLU36_07580"
     CDS             4152..5111
                     /locus_tag="PLU36_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HMZ46648.1"
                     /translation="MTTHQIVYLVFAVVLVVALIFDLGLLNKKNKEVSIKAALQQTML
                     WVSLALGFFVFMWVEEGQTLALQYLSAYLMEWSLSIDNIFVFILIFSAFSVKEKHYGR
                     VLLIGILMAIVFRIIFITVGVALVDRFEWILYIFGAFLLYTGIKMFTANHDEEFKPQE
                     SKIYRFMQRFLPLVPHDGGGKYIIREKGKPVYTTLFVVVIMLAAIDLVFALDSIPAVM
                     GISRDKLVIYTSNIFAVLGLRSLFFLLRGAVSKFDYLQQGIAIVLVFIGIKMLAEHYI
                     SMVVSKNIQVAISLGVILVCISGSIFYSIFMKKKGVPKDVEEE"
     gene            5124..>5614
                     /locus_tag="PLU36_07585"
     CDS             5124..>5614
                     /locus_tag="PLU36_07585"
                     /inference="COORDINATES: protein motif:HMM:NF013396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HMZ46649.1"
                     /translation="MSYTIHNIAIILKATATINNNTLIEQLLIDSRKITFPTASLFFA
                     LHSKVKNGHSFINEVYERGVRNFVIDEDIDSTPFTNANFLKVPNTLVALQALATHHRN
                     EFNQLQVIGITGSNGKTIVKEWLNQLLQTDFNIVRSPRSYNSQIGVPLSVWQINSTNT
                     LGI"
BASE COUNT         1948 a          743 c          906 g         2017 t
ORIGIN      
        1 aatggtatgc aacgtgctaa ttatgctaca gaacatgatg cgtttgttgc taaaaaatta
       61 gcttatgtaa tgtgtggtgg cgatttaagt gaaccacaat ttgtaactga acaatattta
      121 ttggatttag aaagagaagc ttttttaagc ctttgcggcg aaaagaaaac tttagaaaga
      181 atacagggag ttttaaaaaa tggcaaacct gtaagaaact aagatagtct gaccgcctcg
      241 gtctgcctat cttagtttct catctttata ttacagtcag accgtctcgg acatactgta
      301 aatttcttca ggccttcact gcttgcctat cttagttttt caccttttat tatattacag
      361 tctgaccgtc tcggtctgac tgtaaatttt ttatgacctt ctcggtcaga ctatcttagt
      421 ttctcatctt tatattacaa tcagaccgtt tcggtcagac tgtaaatctc ttatcttgta
      481 ttatatatca gtcaaaatat ttggagcaaa ctgttgtaaa tttgttacac tatgcatttt
      541 gttcccaata acctctacca catatacaat cagggaaata ataaacaagt aatatttcat
      601 gatgaggatg attactatac ttttttacga ttaacaagaa atttatttca ttctgtttgt
      661 gatattgttg catattgctg tatgcctaat cattttcatt ttataattag tacaaatgga
      721 tattcttgtg aaataaaaca acaaggtaat attagcattg atgctattac caattcaatc
      781 agaaaacttt tgagcagctt tgctaggcta tacaataaaa agtatctaca atctggctcg
      841 gtgttcagac agaaaacaaa atctaaatgc ttaacaaatt gcgatataga ggttttaaca
      901 tcaatgaatg gtagagaata tttgtttact tgttttcatt atgttcatca aaatccatta
      961 agagcaggat tagtaaataa gttggaggat tgggattttt catcgtttaa agattatgca
     1021 ggtttaagta gtgggacttt gataaataaa gaagtagcaa aattttatga tatttataat
     1081 ccttccaatt ttataaaaga atcatatatg gtaataagcg atgaatatat caaattcata
     1141 gtttgaccaa ctcggcctga ctatgttggt ttttcatctt acattatcta tcagtatgtc
     1201 agtcagaccg ggacggtcag actaatagca taaacttaaa cttcttttca gtcttctcgg
     1261 taagaccgtc tcggtctgac tatggaagga ttgagtaaac aataatgtaa tagcttaatt
     1321 cattacaaac ttttctgttt gttctttgcc atccatagaa aatatgctta tggattttgg
     1381 tttttcttct tctacataaa taatgttgct agtaggagta agacttatta taatttcttt
     1441 tgaagaaaaa ggttttacag ttacactatc tttcatattt taaaaaatta aaatgatcaa
     1501 aatattctct ataacataat tgttttggtc tttaaataaa taagactatt gtttttgttc
     1561 tgcatataat tctgaagtaa caaatgaagt aattgttaaa tttgatttaa tttagttgta
     1621 aacttttttc ttttaaaata agctactcaa tcttttatca tttagtttta caaacttatg
     1681 ttgatgattt gtaaaaaaga atggcagcag ttttttagta acctaacagg ctatataatt
     1741 attgctgttt tcttaattgt aaatggcttg gtgctatttg tttttgatga taacattttt
     1801 tcttttggat atgccacctt agaaaaattt tttatgcttg caccatgggt attgttatta
     1861 ctaatacctg ccgttacaat gcgtagtttg cccgatgagt ataaattagg tactatagaa
     1921 gttttaaaaa ccctaccact tacaccatgg caaataataa caggtaaata ttttggtaat
     1981 ttattggtaa tttttctttc cttattacct acgtttattt acattttttc aatacaaaac
     2041 cttgctgacg gaaatggttt agatttagct gcaactattg gctcttatat tggattgttt
     2101 tttttagctg cagtgtttac agctattggt atatgtgcag gtagttttac aaataatgct
     2161 gtggtagctt ttataattag cctttttgct tgtgccttac tgtattatgg ttttaatgcc
     2221 atcagtaaat tatctcaatt atctggcggt atagattatt atttagaaat ggcaggaata
     2281 gattttcatt acaagaacat tagtaagggt gttatagata ctagagatgt tgtttacttt
     2341 ttgagtatta tttttttatt actgttaatt acagtgagaa acttaaaaca taaaatataa
     2401 ttagctaaac ttagtattag attattagaa aaatgcaaaa actattacaa cataaattta
     2461 gttggctttt actaattgca gcatttgcca ttgttaattt tatggcaagt atcgttcata
     2521 ctaaaataga tttaactaaa gaaaaaagat ataccattag taaagccaca aaagacattc
     2581 tacaacatat tgatgataaa cttgaaataa ctgttttttt agaaggaaat ttacctgctg
     2641 gttttcaaaa attggcaaac actaccaacg attttttaca attattgaaa gaaaaaaata
     2701 atgctaagat tacatttcaa tttatatctc cagataccga agtagatggc accaacataa
     2761 aatttgaaga tactttagta gcaatgggtg caacaccaat taatcttaca atacaagtaa
     2821 aacaagggca agagagtaaa gtagtttttc cttatgcact aataaaatat aaaacgcata
     2881 ctcaattaat aagtttatat agtggtaata gcagaatgat ttctcaagat gaaattaaca
     2941 atgctgaagc aatgatggaa tacaaatttt taaaagcatt tgatgcattt gtttttcctg
     3001 aaaaaccttt agtagcttac tcttttgcaa atggagaacc aacagatatt agaacttacg
     3061 atttactaca aacattacaa aaagattata gtttatatac gtttgatatt gctcaaaggc
     3121 cagcaatacc agatactttt aaagtactaa tgatcgtaaa accatcttta cagtttagcg
     3181 aagatgaaaa attgaaaata gatcaatacc tcatgcgtgg aggaaaaatt ttgtgtttta
     3241 ttgataatct aaatgcagag caagatagtt taaattttaa gccagaatta gtggcttatg
     3301 atagaaacct taaccttaca gacttattgt ttaaatatgg ggtaagaatt aatacggatt
     3361 tagtaatgga tcttcaatgc aattttatgc cttttgctgt gggtggtgca aaagaaaacc
     3421 ctcaatacga atttttgcat tggaattatt accctctatt tgaaagtaaa aataatcata
     3481 ctatcaacaa aaatttgggc ttagtatctg gtcgttttgt aaactcaata gatactgtaa
     3541 aagcagaagg tataaccaaa accattttac tttcttcttc tgataattca agaaaattag
     3601 gtacgccagc tttaattagt ttaaatgaaa acagaaatac cccacaagat attttatttc
     3661 aacaaaaaaa tattccggta gcagttttat tggaaggtag ttttacctct ttatacaaaa
     3721 acaggttggc acaacatatt aaagatagtg tagaaaaaat tggttatcct tttttagaaa
     3781 aaagtttctc tgaaagtaaa ataattgtgg tggcagatgg agatatagtt ttaaatgatg
     3841 caagccaaaa acttgggcca ctaccaatgg gcttaaatat gtacactgta ggtacacaat
     3901 atgaatatca atttgctaac agagagtttt tgttaaatag tttagaatac ttaaccaata
     3961 agcaaagtat aattcaatct cgtaacaaag aacaaatttt aagattactg aatacttcta
     4021 aagtagaaga agaaaaagta aaatggcaaa tgattaatat tgggctgcct ttactgtttg
     4081 taattattta tggatgggtt tataacctca tcagaaaaaa gaaattcacc acttaactta
     4141 tattttagtt catgacaaca catcaaattg tttatttagt ttttgccgtt gtactagtag
     4201 ttgcattaat ttttgactta ggcctattga ataaaaaaaa caaagaagtt agtattaaag
     4261 cagcattgca acaaactatg ttatgggtta gtttagctct cggctttttt gtttttatgt
     4321 gggtagaaga aggtcaaacg cttgccttac agtatttaag tgcttactta atggagtgga
     4381 gtttaagtat agataacatc ttcgtattta ttcttatttt ttcggctttt agtgtaaaag
     4441 aaaaacatta tggcagggtt ttattaattg gtattttaat ggctatagtt tttagaataa
     4501 tttttataac tgtaggtgtt gcattggtag atagatttga atggatatta tacatttttg
     4561 gagcttttct tttatataca ggtattaaaa tgtttactgc aaaccacgat gaagaattta
     4621 aacctcaaga aagtaaaatt tataggttta tgcaaaggtt tttaccatta gtaccacacg
     4681 atggtggtgg taaatatata attagagaaa aaggtaaacc tgtatataca acattatttg
     4741 ttgtggttat aatgttagct gctattgatt tagtttttgc tttagatagt attcctgcag
     4801 taatgggtat aagcagagat aaattggtga tttatacttc taatattttt gctgttttag
     4861 ggttaagaag tttattcttt ttattgcgtg gagcagtttc taaattcgat tatttgcaac
     4921 aaggtattgc cattgtattg gtttttattg gtataaaaat gttggcagag cattatatta
     4981 gcatggtagt aagtaaaaat attcaagtag caatatcatt aggagtaatt ttagtatgta
     5041 tcagcggctc tattttttat tctattttta tgaaaaagaa aggtgtacca aaagatgttg
     5101 aagaagagta gcttaataat aacatgagtt atacaataca taatattgca attattttaa
     5161 aggccactgc aactattaat aacaatactt taattgaaca attattaatt gatagcagaa
     5221 aaataacctt tcctacagca tctttatttt ttgctttaca cagcaaagta aaaaatggac
     5281 attcctttat aaatgaagta tatgaacgag gtgttagaaa ttttgtaatt gatgaagata
     5341 ttgatagtac accttttacc aatgctaatt ttttaaaagt accaaacaca ttagtagcgt
     5401 tacaggctct agcaacacat cacagaaatg aatttaatca attacaagta attggaatta
     5461 caggtagtaa tggaaaaact attgtgaaag aatggctaaa ccaattacta caaacagatt
     5521 ttaatatagt aagaagtcca agaagttata actcgcaaat tggtgtacca ttaagtgtat
     5581 ggcaaattaa ctctactaac actttaggca tttt
//
LOCUS       DAOQPK010000147         5582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_8773_length_5582_cov_4.269948, whole genome shotgun sequence.
ACCESSION   DAOQPK010000147 DAOQPK010000000
VERSION     DAOQPK010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5582
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8773_length_5582_cov_4.269948"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="PLU36_07590"
     CDS             complement(<1..798)
                     /locus_tag="PLU36_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HMZ46650.1"
                     /translation="MQKLTLAIHGGAGTILKNDLTPELERLYNDGLKQALQSGYAVLE
                     KNGTATDAVVAAVTSLENNILFNAGKGSVFTKNGEHEMDASIMEGKNLIAGAVAGVKN
                     IKNPIQLAQKIMLHSNHVFLSGQGAFEFAQQQQLAVENEDYFYSEFRYKQWQAVKETD
                     NYLLDHNSNESENLFKNKKFGTVGATACDAFGNLAAATSTGGMTNKQFNRIGDTPVIG
                     GGTYANNNTCAISCTGHGELFIRAVAAYDVSCLMEYKNCSLQQAMDIV"
     gene            853..1551
                     /locus_tag="PLU36_07595"
     CDS             853..1551
                     /locus_tag="PLU36_07595"
                     /inference="COORDINATES: protein motif:HMM:NF040120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HMZ46651.1"
                     /translation="MQENLFFKLFKNKKLTATNYLFIAINLIPIWGVWFKNWDARIIF
                     IIFCLETVIIGLYNILKIFTASIFSNKNEIVNNSKVPKLAVGLFIIAFFIFHFGLFVF
                     VQMKIFLGVLSMNKMHIEMYDLFFNLRSTLPRYAQLLLLLFFISYGFGVIKEFVIPKQ
                     FKTVRLEKLMLEPYGRIITQQLVVIIGTFTLFINKDGKILILIFVLVKLYFEVFMDYK
                     VFLKDSYKNNTTSL"
     gene            1593..3092
                     /locus_tag="PLU36_07600"
     CDS             1593..3092
                     /locus_tag="PLU36_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ46652.1"
                     /translation="MQSEKGIWKVIAASSVGTLIEWYDFYIFGSLAIIISEKFFPPDN
                     STTAFLATLATFAAGFIVRPFGALFFGRLGDMIGRKYTFLLTLIIMGGSTFLIGCIPS
                     YQTFHFAPYVILFLRLLQGLALGGEYGGAATYVAEHAPEHKRGYYTSWIQTTATLGLL
                     VSLVVIITTQNSLSKEDFNNWGWRLPFLLSIVLVLVSIIIRLKMKESPLFAKLKTEGK
                     TSINPIKESFGKRQNLKLVLLALFGATMGQGVIWYTGQFYTQTFLLKTCNLNAIQTNT
                     WLGVALILATPFFIFFGWLSDKIGRKVILLTGMLLAVFLYRPIFSELYSITNTKNKQE
                     ITTNIHISKTVVLNNTADSLITKTTTTNFTDKTVLIETEKYVESVVKTGNNQPKESKK
                     IITLDSHYKWKIIGLIFILVFFVTMVYGPIAAFLVELFPTKIRYTSMSLPYHIGNGVF
                     GGLVPFIATLITVMPGSNYLSGLWYPIGIGLICFIIGLVYLSNNQVKNS"
     gene            3102..3323
                     /locus_tag="PLU36_07605"
     CDS             3102..3323
                     /locus_tag="PLU36_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46653.1"
                     /translation="MQKIKKYLGIVWIILAILSVIILIITAIENIASKGGADIHNPIP
                     WIIIITIYLPIAFGFALFGYYAFKEEYHV"
     gene            3363..4298
                     /gene="fmt"
                     /locus_tag="PLU36_07610"
     CDS             3363..4298
                     /gene="fmt"
                     /locus_tag="PLU36_07610"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HMZ46654.1"
                     /translation="MNYKDLRIVFMGTPEFAVASLNALVNAGCNIVAVVTAPDKPAGR
                     GLQLNESAIKKYAVANNLKILQPVKLKSVDFIETLKSLKADVQIVVAFRMLPEMVWNM
                     PTMGTINVHGSLLPQYRGAAPINWAVINGEKFTGVTTFKLKHAIDTGNILLQESLAID
                     ENETAGEVHDKMKELGAELLLKTIQQLAEGKLIEQEQNKLNNTLTLKHAPKLYSENGK
                     IDFSKTVEEVHNLVRGLSPFPGAYTFLNNKLLKIYRTIKEVSDEPLEVGSIKSDGKKY
                     FKMACCNGYINILELQLEGKKRMKIEDFLRGNKIQ"
     gene            complement(4308..4680)
                     /gene="ssrA"
                     /locus_tag="PLU36_07615"
     tmRNA           complement(4308..4680)
                     /gene="ssrA"
                     /locus_tag="PLU36_07615"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(4772..>5582)
                     /locus_tag="PLU36_07620"
     CDS             complement(4772..>5582)
                     /locus_tag="PLU36_07620"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HMZ46655.1"
                     /translation="ASQIILSNASNISIVRNGENMQLNVTEEFLTKLKKNKGNGIASI
                     RIPFIIDTVMAGQGAAKAGLKKNDYIIAVDSIATPYFNDFQNVIVNYASKTVPFKVIR
                     NKDTLTLPVTLSDNAKIGAGTKGLKDLGFEIDVKNYSLLEAIPVGFNLCWSSLDKYIE
                     GIKRLFQGKDKVSDSLGSVISIGSIYSKVWDWQNFWTLTALFSIILAFMNILPIPALD
                     GGHALFCIVEMVTGKKPNDKFMEYAQTVGMFLLLGLMVYALGLDFLRLFKG"
BASE COUNT         1941 a          856 c          845 g         1940 t
ORIGIN      
        1 aacaatatcc atagcttgtt gcaaactaca attcttgtac tccattaaac aacttacatc
       61 gtatgcagca actgctctaa taaacaattc tccatgccct gtacaactaa ttgcacatgt
      121 gttgttattg gcataagtac caccacctat aacaggtgta tcgccaattc tattaaattg
      181 tttgttggtc attccgccag tacttgttgc agctgcaagg ttgccaaaag catcgcaagc
      241 agttgcccct actgttccaa attttttatt tttaaataaa ttttcagatt cattactatt
      301 gtgatctaac aaataattat ctgtttcttt tactgcttgc cattgtttat atctaaattc
      361 agaataaaaa taatcttcat tctctactgc taattgttgt tgttgagcaa actcaaatgc
      421 tccctgccca cttaaaaaaa catggttgct atgcagcata attttttgtg caagctgaat
      481 tggattttta atatttttca cacctgcaac tgcaccagct attaaatttt taccttccat
      541 aatagaggca tccatttcat gttcgccatt ttttgtaaac actgatccct tacctgcatt
      601 aaacaaaata ttattttcta atgaagttac agcagcaaca actgcatctg ttgcagttcc
      661 atttttttct aatacagcat aaccgctctg caatgcttgt tttaagccat cattatataa
      721 tctttcaagt tcaggggtta agtcgttctt caaaatagtt cctgcaccac cgtgtattgc
      781 tagtgtaagt ttttgcatgt ataatttttt cattaatttg gaaacacaaa tcaaagaaaa
      841 aaagttcact tcatgcaaga aaatttattc tttaaacttt ttaaaaataa aaaattaacc
      901 gctaccaact acttgtttat agccattaat ttaataccta tatggggcgt ttggtttaaa
      961 aattgggatg ctagaataat ttttataatt ttttgtttag aaacagtaat aattggcctt
     1021 tacaatattc taaaaatatt tactgcctcc atattttcta ataaaaatga aattgtaaac
     1081 aatagtaaag tgccaaagct agcagttggc ttatttatca tagcgttctt tatttttcat
     1141 tttggattat ttgtttttgt acaaatgaag atatttttag gtgttttaag tatgaacaaa
     1201 atgcatattg aaatgtatga tttgtttttt aatttaagaa gcactttacc tcgttatgca
     1261 caactgctat tgttattatt ttttataagt tatgggtttg gtgtaataaa agaatttgta
     1321 atacccaagc aatttaaaac agttagatta gagaagttaa tgcttgaacc gtatggaaga
     1381 ataattacgc aacaattagt agttattatt ggtacattta ctttgtttat aaataaagat
     1441 ggaaaaatat taatactcat ttttgtttta gttaaattgt attttgaagt ctttatggac
     1501 tataaggttt tccttaaaga ttcttacaag aataatacaa ccagtcttta aattacatac
     1561 aacctaatta gattaaataa caataaaaat ttatgcaatc agaaaaagga atttggaaag
     1621 ttattgcagc atctagcgtt ggtacattaa tagaatggta tgatttttac atatttggaa
     1681 gtttagcaat tattatttct gaaaaatttt ttccgccaga caactccaca acagcatttt
     1741 tagcaacatt agcaaccttt gcagcaggct ttattgtgag gccatttgga gctttatttt
     1801 ttggaagact tggcgatatg attggtagaa aatatacttt tttactcacc ttaattatta
     1861 tgggtggaag tacattttta attggatgta ttccatctta tcaaactttt cattttgcac
     1921 cttatgttat tttattttta agattactac agggcttggc attaggaggt gaatatggtg
     1981 gtgctgcaac ctatgtagca gagcatgcac ctgaacataa aagaggctac tatacaagct
     2041 ggattcaaac cactgcaaca ttaggcttat tggtttcttt agtagttatt attactactc
     2101 aaaacagctt aagcaaagaa gattttaata attggggttg gcggctacct ttcttattaa
     2161 gtattgtgtt ggtattagtg tccattatta taaggctaaa aatgaaagaa agccctttgt
     2221 ttgctaaact aaaaacagaa ggcaaaacga gcattaatcc cattaaagaa agttttggaa
     2281 aaaggcaaaa tttaaaatta gttttattgg ctttatttgg tgcaacaatg gggcaaggtg
     2341 taatttggta cacagggcaa ttttacactc aaacattttt attaaaaact tgtaatctaa
     2401 atgctattca aaccaatact tggcttggag ttgcattaat attagcaact ccatttttta
     2461 ttttctttgg ctggctgagc gataaaatag gaagaaaagt aattttgtta acaggtatgc
     2521 tattggctgt atttctttat agacctatat tttctgaatt atactctatc actaatacaa
     2581 aaaacaaaca agaaataacc acaaatattc acataagcaa aacagtagtt ttaaataaca
     2641 ctgccgattc gttaataact aaaacaacaa ctacaaattt tactgataaa actgtactaa
     2701 ttgaaacaga aaaatatgta gaatctgtag taaaaactgg aaataaccaa cccaaagaat
     2761 ctaaaaaaat aattacactg gatagccatt ataaatggaa aataattggt ttaatattta
     2821 ttttggtatt ttttgtaacc atggtttacg gcccaattgc agcattttta gtagaactct
     2881 ttcctactaa aataagatat acttcaatgt ctttacctta tcatattggt aatggtgttt
     2941 ttggtgggct tgtacctttt attgcaacat taataactgt tatgcctggt agtaattatt
     3001 taagtggctt atggtaccca atcggtattg gattaatttg ttttattatt gggttagtgt
     3061 atttaagcaa caatcaagta aaaaattcat aattttaatt tatgcaaaaa ataaaaaaat
     3121 atttaggcat agtttggata atacttgcaa tactttctgt aattatttta ataataactg
     3181 caattgaaaa tattgcctca aaaggtggtg cagatattca taatccaata ccttggataa
     3241 taataattac tatttactta cccattgctt tcggttttgc cctatttggt tattatgcat
     3301 ttaaagaaga ataccatgta taattataag actagtatta atagctgctt tacttttgct
     3361 gcatgaatta taaagattta agaattgttt ttatgggtac gccagagttt gctgtagcat
     3421 cgttaaatgc tttggtaaat gcaggttgca atattgttgc tgttgtaact gcacctgata
     3481 aacctgctgg acgtgggttg cagcttaatg aaagtgctat aaaaaaatat gctgttgcaa
     3541 ataatcttaa aatattacaa cctgtaaaat taaaaagtgt agattttatt gaaacattaa
     3601 aaagtttaaa agcagatgta caaattgtag tagcctttag aatgttacct gaaatggttt
     3661 ggaatatgcc tactatggga accataaatg tacatggaag tttactgcct caatacagag
     3721 gggcagcacc aataaactgg gcagttatta atggagaaaa gtttacaggc gttactacat
     3781 ttaaactaaa acatgcaata gatactggca atattttatt acaagaaagt ttagccatag
     3841 atgaaaatga aactgcagga gaagtgcatg ataaaatgaa agaacttgga gcagagttgt
     3901 tattaaaaac aatacaacaa cttgcagaag gcaaattaat tgaacaagaa caaaataaat
     3961 tgaataatac attaaccttg aaacacgcac caaagcttta ttctgaaaat gggaaaatag
     4021 atttctctaa aacagtggag gaagtacata atttggttag aggcttatct ccttttcctg
     4081 gtgcatatac ttttttaaat aataaattat taaaaattta tagaacaatc aaagaagtaa
     4141 gtgatgaacc attagaggta ggtagcataa aaagcgatgg aaaaaaatat tttaaaatgg
     4201 cttgttgtaa tgggtatata aatattttag agttacagtt agaaggtaaa aaaagaatga
     4261 aaatagaaga ttttttgaga ggaaataaaa ttcaataagc attaaaaaag tggagccgaa
     4321 gggagtcgaa cccttgtcca aacaaattcg ccataagttt tctacatgct tattactgca
     4381 ttaattgtag ggaaaataca ggggcagcac caaccaatat tatccttagc tgaatgatct
     4441 taatttacta tcacagcctt tagcaaaagc aacttttatt tgttttgagt cggcggcggt
     4501 acttggtaaa aagtaaacct gtacggcggc ccaaatgact aactaatcac tgattaggca
     4561 gccatggcat actgagtatt gccatttaaa gtttgaatat tcagattaaa gtgctaatta
     4621 tacaacgcac tgcatgctta tacttacaaa gaactttgct gtcaaaacca atacggcccc
     4681 aaacatttat tttactgtaa caacaataat tttaaggtag aattgttaca aaaaacattc
     4741 caatatcaac taattttttt atttaaataa cctacccttt aaacaatctt aaaaaatcta
     4801 aacctaatgc atacaccatt aaacctaata ataaaaacat gcctacagtt tgtgcatact
     4861 ccataaattt atcgttaggt ttttttcctg taaccatttc aactatacaa aataatgcat
     4921 ggccaccatc taaagcaggt ataggtaaaa tattcataaa tgctagtatg atagaaaaaa
     4981 gtgcagttaa agtccagaag ttttgccaat cccacacttt gctataaata ctaccaatac
     5041 ttattacact acctaggcta tcacttactt tatcttttcc ttgaaatagt cttttaattc
     5101 cttcaatata tttgtctaaa cttgaccaac ataaattaaa gccaacaggt atagcttcta
     5161 ataacgaata atttttcaca tctatttcaa agcctaaatc ttttaaccct ttagttcctg
     5221 caccaatttt tgcattatcg ctaagtgtta caggcaaggt taaagtatcc ttatttctaa
     5281 ttactttaaa tggaaccgtt ttagaggcat agtttacaat tacattttga aaatcgttaa
     5341 agtatggcgt tgcaatacta tctactgcaa taatataatc attctttttt aacccagctt
     5401 ttgctgcacc ttggcctgcc attactgtat ctattataaa tggaatacga atacttgcta
     5461 tgccattgcc tttattcttt tttaatttag ttaaaaattc ttcggttaca ttcaattgca
     5521 tattttcacc atttctaact atagaaatat tggaggcatt acttaatatt atttggcttg
     5581 ca
//
LOCUS       DAOQPK010000148         5561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_8819_length_5561_cov_4.256992, whole genome shotgun sequence.
ACCESSION   DAOQPK010000148 DAOQPK010000000
VERSION     DAOQPK010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8819_length_5561_cov_4.256992"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PLU36_07625"
     CDS             complement(<1..220)
                     /locus_tag="PLU36_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46656.1"
                     /translation="MSNLSLFEKLTLQDEKNLLIQGVPSGIEKQFSKLSYAKNVTPLL
                     KIKKVDFALVFAVNKIQLNNVVREVLCVL"
     gene            complement(361..1179)
                     /locus_tag="PLU36_07630"
     CDS             complement(361..1179)
                     /locus_tag="PLU36_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HMZ46657.1"
                     /translation="MNYKLISLSVVIATLLIACQTNSITGRSQLSLVSETEVQTMAKT
                     QYRQFLSQSKVVSTNTSKDAEMVNRIGKRISTAITKYYTEQGLSNELIGYEWEYHLVD
                     SKEVNAWCMPGGKIVVYTGLLPVTQNEAALAVVMGHEVAHALARHGNERMSQGLLQQL
                     GGVALSTALSNKPQQTQNLFMSAYGISSQVGFMLPHSRKQELEADKFGLRYAALAGYN
                     IREAIPLWERMEKMSAGQKTPEFLSTHPQEERRIKDLQKIMDETIRDYYRPISK"
     gene            1289..2422
                     /gene="tgt"
                     /locus_tag="PLU36_07635"
     CDS             1289..2422
                     /gene="tgt"
                     /locus_tag="PLU36_07635"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HMZ46658.1"
                     /translation="MPQLSFQLQHTENLARAGKIITDHGEILTPIFMPVGTVGSVKAV
                     TQQQLCIDVQAQIILGNTYHLYLRPGVDILEAAGGLHKFNGWHKPILTDSGGYQVFSL
                     AANRKITEDGVLFQSHIDGSRHLFTPEKVMDIERSIGADIIMAFDECPPYPSNYTYAQ
                     KSMELTHRWLQRCINRLSETPDKYGYTQNLFPIVQGSTYKDLRKASCDFVSSLHTTGN
                     AIGGLSVGEPIEMMYEFTALCCENLPYQKPRYLMGVGTPWDIIECIALGVDMFDCVMP
                     TRNGRNGMLFTTQGVINIKNKKWATDFSPIDTGLPSEISNFYSKAYLRHLFVADEILG
                     LQIASLQNLSFYLWLVNEARNQILSNNFYSWKNNILPIIKQRL"
     gene            complement(2433..3659)
                     /locus_tag="PLU36_07640"
     CDS             complement(2433..3659)
                     /locus_tag="PLU36_07640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HMZ46659.1"
                     /translation="MLQTDICIIGAGPAGATTSLFLSKMQIPHIIVDASVFPRDKICG
                     DALDLKVMRILNCLENDFVKNEILHNHNFSKTYGVKINISENKKPLLQYKPKPNEHQF
                     PYFFFSKRRYFDNFLAERIPSKYATFLQGTKVEKIEHTEEGKIIYTNNVKEANKIKAK
                     LIVGADGDHSVVLRSLGERSVNREHYAGGLRQYWKGIEGISSEDNFLELYLPKSLPLA
                     YLWIFPLPNGEANVGCGLLSSLIQKNNVNLKNLLQDIITKDPVLAYRFRNAVPLEKPM
                     GWGLPMATLKRKASGNGWLLTGDAASLINPLTGEGIGPGMMSGYIAAHFIQRAVKQNN
                     FSEKLFKNYDREINRRLEKSITNFNLIKKVSPLIYNFFINVVAPHPLCRLYFQRNVGK
                     WIKTAYDNKPIKIKFD"
     gene            complement(3702..4442)
                     /locus_tag="PLU36_07645"
     CDS             complement(3702..4442)
                     /locus_tag="PLU36_07645"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphatase"
                     /protein_id="HMZ46660.1"
                     /translation="MFGLFKKNKTHPDLSFIGVDMHSHLLPGLDDGLKTIEDTIAFVQ
                     ELNTLGYEKLICTPHILSAVHPNTPQTILPKLEEVQKAVKEKNIKIQIEAAAEYMVDH
                     EFEQLIKSGNKLLTFGTNFILIEMSYLAPSPNLGQVIFDLRLAGLQPILAHPERYNYY
                     HHQFHEFKKIKDTGCWFQVNMLSLAGYYGKHIKKTAEKLIAEGMIDLIGTDMHHQNHL
                     HATKQLALSNYFYKILKDVPLKNRMFLD"
     gene            complement(4452..>5561)
                     /locus_tag="PLU36_07650"
     CDS             complement(4452..>5561)
                     /locus_tag="PLU36_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HMZ46661.1"
                     /translation="RDKKILITGAAGSIGSELARQIASVYPSSLILCDQTETGLYELE
                     YELQQKYSIGNALKVYLGDVKDKCSMQNLFSNYSPEVVFHAAAYKHVPMMENHPSEAI
                     RNNVLGTKTVADFAEQFGVDTMVLVSTDKAINPTNVMGASKRIAEMYCQALQNRSELP
                     IWDSGIVKMGNHQLKRTKFITTRFGNVLGSNGSVIPRFQEQITNGGPVTVTHPDIIRY
                     FMTIPEACSLVLEAGTMGKGGEVFLFDMGEPIRILDLAKKMIRLAGLTPGKDIELRFT
                     GLRPGEKLFEELLNKEEEVIPTHNKKILIAKVIEFEFSKVSESINMLISLANQNQDED
                     VVKQMKFIVPEFISNNSIYESIDAQMFKPLYSAIR"
BASE COUNT         1770 a          923 c          824 g         2044 t
ORIGIN      
        1 gtagtacaca caatacttct cttactacat tatttaattg tattttgttt actgcaaaaa
       61 ctaatgcaaa atctaccttc tttattttta aaagaggagt tacatttttg gcataagata
      121 gtttagaaaa ttgcttttca attccagaag gcacaccttg aattaaaagg tttttttcgt
      181 cttgtaatgt taatttttca aataaactta agttagacat atcctaaaat tggtttttca
      241 ggtgatgtaa aaaaagaagt gcaaagttat aataatttat tgattttttt tagtctatta
      301 aagatagaaa tgtaatttta acaaccagta ttttttaacc tattgaaaat cagtaataaa
      361 ttatttggat atgggtctat aataatctct aatagtttca tccattattt tttgaaggtc
      421 ttttattctt ctttcctctt gtgggtgagt acttaaaaac tctggtgttt tttgccctgc
      481 actcattttt tccatccttt cccaaagtgg tattgcttct ctaatattat aaccagctaa
      541 tgctgcatat cttaatccaa atttatctgc ttctaattct tgtttacggc tatgtggcaa
      601 cataaagcca acttgtgaac tgataccata agcagacata aataaatttt gagtttgttg
      661 tggtttatta gataaagcag tacttaatgc aacacctcct aattgttgta ataatccttg
      721 gctcattctt tcattaccat gccttgccaa tgcatgagca acttcgtgcc ccattacaac
      781 agccaatgct gcttcatttt gtgttactgg caacaaacca gtataaacaa caattttacc
      841 acctggcatg caccaagcat ttacctcttt actatctacc aaatgatact cccattcgta
      901 gcctataagt tcattgctta atccttgctc tgtataatat tttgtaattg cagtagaaat
      961 tcttttacca attctattta ccatttcagc atctttacta gtatttgtac taacaacttt
     1021 actttggctt aaaaactggc ggtattgtgt ttttgccatt gtttgcactt ctgtttcgga
     1081 tactaaactt aattgcgacc tacctgttat tgagtttgtt tgacaagcaa ttaatagcgt
     1141 agctattaca acacttaaac ttattaactt ataattcata atttataaaa tagtttgcaa
     1201 aaataatcaa taaaaaaata tgccccattt ctgaaaataa ttttaatata aattgttata
     1261 caatttaaaa ttatattttt gttggtttat gcctcaactt tcatttcaac tacagcatac
     1321 tgaaaattta gcaagagccg gaaaaattat tacagaccat ggcgaaatac ttactccaat
     1381 ttttatgcca gttggtacag taggtagcgt aaaagcagtt acgcaacaac aactttgtat
     1441 tgatgttcag gctcaaataa ttttaggcaa tacttatcat ttatacttac gcccaggtgt
     1501 agatatttta gaggcagccg gaggcttaca taaatttaat ggttggcata aacctatatt
     1561 aactgatagt ggtggatatc aggttttttc gttggctgct aacagaaaaa ttacagaaga
     1621 tggggtttta tttcaaagcc atattgatgg tagtaggcat ttgtttacac cagaaaaagt
     1681 aatggatatt gagagaagca ttggtgcaga tataattatg gcttttgatg aatgcccacc
     1741 ttaccctagt aattatacct atgcacaaaa aagtatggag ttaactcata gatggttgca
     1801 gcgttgcata aacaggttat cagaaacacc tgataaatat ggctatactc aaaatttatt
     1861 tccaattgta caaggtagca cttataaaga tttaagaaag gcttcttgcg attttgtaag
     1921 tagtttgcac acaacaggca atgccattgg tggactaagt gttggagaac ctattgaaat
     1981 gatgtatgaa tttactgctc tttgctgcga gaatttacct taccaaaaac ctaggtactt
     2041 aatgggtgtt ggtaccccat gggatattat tgaatgtata gctttagggg ttgatatgtt
     2101 tgattgtgtg atgccaacaa gaaatggaag aaatggaatg ttatttacta cacaaggtgt
     2161 tattaatata aaaaataaga aatgggcaac agatttttcg ccaattgata ctggcttgcc
     2221 ttcagaaatt agtaattttt attctaaagc ttatttaagg catttatttg ttgcagatga
     2281 aatattagga ttacaaattg caagtcttca aaacctatct ttttacttat ggttagttaa
     2341 cgaagccaga aatcaaatat taagtaataa tttttatagt tggaagaata atattttgcc
     2401 aataattaag caacgtttat aaaattttat cttcaatcga attttatttt tataggctta
     2461 ttatcataag cagtttttat ccatttacct acatttcttt gaaaatataa acgacataaa
     2521 ggatgtggag ctaccacatt tataaaaaaa ttataaatta aaggagatac tttttttatt
     2581 agattgaagt ttgttatact tttttctagc cttctattta tttctctatc ataattttta
     2641 aataatttct cagaaaaatt attttgtttt acagcacgct gaataaagtg tgcagcaata
     2701 tacccactca tcattcctgg accaattcct tctcctgtta aaggattaat taacgaagct
     2761 gcatcgcctg taagtaacca accattacca cttgcttttc tttttaaggt tgccataggt
     2821 aagccccaac ccattggttt ctctaatggt actgcatttc taaatctata agctaaaaca
     2881 gggtctttag taataatatc ctgcaaaagg tttttaaggt ttacgttatt tttttgtatt
     2941 aagctgctca gcaacccaca accaacattg gcttcaccat ttggtaacgg aaaaatccat
     3001 aaataagcta atggtaagct tttaggtaaa tacaattcta aaaaattatc ttctgaagat
     3061 ataccttcta tacctttcca atactgtctt aaacctccag cataatgttc tctgttaaca
     3121 cttctttctc ccaaacttct taaaactact gagtggtcgc catcggcacc aacaattaat
     3181 ttggctttaa ttttgtttgc ctcctttaca ttattggtat aaataatttt accttcttct
     3241 gtatgttcaa ttttttccac ttttgtaccc tgcaaaaagg tagcatattt ggaaggtatt
     3301 ctttctgcta aaaaattatc aaagtatctt cgtttactaa agaaaaagta aggaaattgg
     3361 tgttcgtttg gttttggttt gtattgtaat aatggctttt tattttctga aatattaatt
     3421 tttacaccgt atgttttaga gaaattatgg ttatgcagta tttcattttt tacaaaatca
     3481 ttctctaagc aatttaaaat acgcattact ttaaggtcta gagcatctcc acaaatttta
     3541 tctctaggaa aaacagaagc atctacaatt atatgcggta tttgcatttt tgataaaaac
     3601 aaagaagttg tagcacctgc tgggcctgct ccaataatgc aaatatctgt ttgtagcatt
     3661 ttaaatgatg acgttaaatt tagaggcaaa taaataaaac tttaatcaag aaacattcta
     3721 ttttttaagg gcacatcttt taaaatttta tagaagtagt tactcaatgc caattgtttt
     3781 gttgcatgta aatgattttg atggtgcata tcggtaccaa ttaaatctat cataccttcg
     3841 gcaattagtt tttctgctgt tttttttatg tgtttgccat aatagccagc caacgataac
     3901 atatttactt gaaaccaaca gccagtatct tttatttttt taaactcatg aaactgatgg
     3961 tgataataat tatatctttc tgggtgtgct aaaataggtt gcaaacctgc taaacgtaaa
     4021 tcaaaaataa cttgccctaa gtttggagag ggtgctaaat aggacatttc aatcaatata
     4081 aaattagtac caaaagttaa aagcttatta ccgcttttta ttagttgctc aaattcgtga
     4141 tcaaccatat attctgcggc tgcttcaatt tgtattttaa tatttttttc tttaactgct
     4201 ttttgtactt cttctaattt tggtaaaata gtttgtgggg tattggggtg cactgcagat
     4261 aaaatatgag gagtgcaaat taatttttca taacccaaag tatttaactc ttgtacaaat
     4321 gcaatggtat cttctatagt ttttaaacca tcatctaacc caggaagtag atgagagtgc
     4381 atatcaacac caataaacga taaatcgggg tgtgttttat tttttttgaa caatccaaac
     4441 atgcagcaaa tttacctaat agcagaatat aaaggtttaa acatttgtgc atctatgctt
     4501 tcataaatag agttattact aataaactca ggaactataa atttcatttg tttaacaaca
     4561 tcttcatctt ggttttggtt tgctaaactt ataagcatat taatgctttc agaaacttta
     4621 gaaaattcaa actctattac tttagcaatt aaaattttct tgttatgtgt cggaattact
     4681 tcttcttctt tattcaataa ttcttcaaat agtttttctc caggtcttaa accagtaaat
     4741 cttagttcaa tgtccttacc tggtgttaaa ccagctaatc gaatcatttt tttagccaaa
     4801 tctaaaattc taatgggttc tcccatatca aataaaaaca cttcgccacc tttgcccatt
     4861 gtacctgctt ctaacactaa agagcaagct tctggtatgg tcataaaata tctaataata
     4921 tctggatgtg ttacagttac tgggcctcca ttagttattt gctcttgaaa acgtggaatt
     4981 acagaaccat tagagcctaa tacatttcca aatcttgttg ttataaattt tgttcttttt
     5041 aactggtggt tacccatttt tacaattcca ctatcccata ttggtagctc gctcctattt
     5101 tgtaaagcct ggcaatacat ttctgcaatt cttttagatg cccccattac attggttgga
     5161 ttgatagcct tatctgttga aaccaacacc attgtatcta ccccaaattg ttcagcaaaa
     5221 tctgccactg ttttagtgcc taaaacatta tttctaatag cttctgatgg atgattttcc
     5281 atcattggta catgtttgta ggcagcagca tgaaatacaa cttctggaga atagttggaa
     5341 aataaattct gcatagagca tttatctttt acatctccta aatatacttt tagtgcattg
     5401 ccaatactat atttttgttg taactcatat tctaattcat ataagccagt ttcagtttga
     5461 tcgcataaaa ttaaggagga aggataaaca ctagcaattt ggcgtgctaa ctcacttcca
     5521 atagaacctg cagcacccgt tattaatatt tttttatctc t
//
LOCUS       DAOQPK010000149         5501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_8954_length_5501_cov_4.942343, whole genome shotgun sequence.
ACCESSION   DAOQPK010000149 DAOQPK010000000
VERSION     DAOQPK010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5501
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8954_length_5501_cov_4.942343"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..1097
                     /locus_tag="PLU36_07655"
     CDS             51..1097
                     /locus_tag="PLU36_07655"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HMZ46662.1"
                     /translation="MANKPVKALSTIAATEPLKVVNIERRTLTDKDIEIEILYCGICH
                     SDLHVAQNDWGGSHYPLVPGHEIVGKVIGVGNSTTKFKINDIVGVGCIVDSCQHCTYC
                     QQGHEQFCEEGWTVVFNSKDKKHGGFTYGGFSTNIIVNEDYVVHIPAGLNLPNAAPIL
                     CAGITVYSPLKHWKVDANKTVGIIGMGGLGHMAIKIAKAMGAYVTVFTTTPTKANDAL
                     QLGADKVIISTNEQEMKHCTKQDIILNTVSAKHNINEYINLLNVDGTIIMVGIPAQPL
                     EIGAYNVIKGRKSFSGSNIGGIAETQEVLNFCAKHNITADIELINPDYINTAFERLSK
                     GDVKYRFVLDMQKI"
     gene            1107..1526
                     /locus_tag="PLU36_07660"
     CDS             1107..1526
                     /locus_tag="PLU36_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46663.1"
                     /translation="MFKYFLFILLLGFYTRTKAQKIEAIYFNLYTDSLKKEVHNYINI
                     EGKLANGKFIPLDSSHIKLSCNTGNWYGNNLVIDSSYLKDSVVITAQLKQNLHLQKQI
                     TVFIKKINEVANLKTEEEVLQSLQPTNNKNKKRKNKR"
     gene            complement(1530..1913)
                     /locus_tag="PLU36_07665"
     CDS             complement(1530..1913)
                     /locus_tag="PLU36_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HMZ46664.1"
                     /translation="MILPVLFISESDNKGKGVFTAQIIPSKTTIEISPVIVLNEKERA
                     IVEQTKLYNNIFEWGENNMKAAIGLGFISMYNHNYLANCVYEMDFENETMSVVTVRQI
                     EAGEELFINYNASFNDTKKVWFDAV"
     gene            1975..3366
                     /gene="mgtE"
                     /locus_tag="PLU36_07670"
     CDS             1975..3366
                     /gene="mgtE"
                     /locus_tag="PLU36_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HMZ46665.1"
                     /translation="MSIEIEQINLQEQFLEIIATQDKILIKEFLDNQNISDVADLIYH
                     NTNDEATIVANMAINRAAKVFKILDFATQKQIINKLPSNKTAELLNELPADDRTDFFE
                     ELPKTVIRDLIKLLDADERKITLSLLGYPEDSVGRLMTPDYVFVYTHNTIEEVLETIR
                     KYAKNSETIDVIYIVNEQDELIDDIRIRDVILASPEKKMEELIDGRYVALHVNDDQEH
                     ASQIFKMNNRVALPVIDDNNKLLGIVTIDDMLWVANEEFSEDMQKMGGTEALNEPYLD
                     ISIFKLFRKRVLWLVILFLGELLTASVMFYFESEIAKAVFLATFIPLIISSGGNSGSQ
                     ASTLIIQAMAVGEIKIADWWRVLRREILSGLMLGTVLGMIGFIRIMIWHEFFPNIYGD
                     HFILVGFVIFFSLIGVVLWGTLSGSMLPLVLKKMGADPAVSSAPFVATLVDVTGLIIY
                     FSFAYLFLQGLIL"
     gene            3429..5303
                     /gene="asnB"
                     /locus_tag="PLU36_07675"
     CDS             3429..5303
                     /gene="asnB"
                     /locus_tag="PLU36_07675"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HMZ46666.1"
                     /translation="MCAIAGIMSFKNNLVELSNLQKMANIMQHRGNDGEGFWLNNSST
                     IGFAHKRLSIIDLSKNAAQPMHYLQHEATNKYTLIFNGEIYNYVELKKDLITKGYQFF
                     TQSDTEVLLALYDCYQENCLQYLDGMFAFALWNNATQQLWIARDRFGEKPLYYNCSDE
                     QFLFASEMKALWAVGVDKTINNARLLNYLSVGMVSNPSNAEETFYNSIKKLPAAHYLI
                     INYNTKEISTKRYWQANLHEEKNLLKEEEVVEKFSYLFAQSIKRRLRSDVAVSTSLSG
                     GLDSSSVIATITALNNKNNNEKPISFSAIFPGFAKDESAFINEVVNYFNLKNYTINPT
                     AEGFVADFEKLLFHQEEPFQSSSIYTQFKVYELAKLHNIKVVLDGQGADEILAGYTKY
                     YFWYWQSLLSNFKFSTLKKEKIAAKKNNQQIDWNSKNIAAAFFPNLAARKLTSNIKNK
                     ILFNPYIEKDFINANFNEQSVQKPTIKNLNQILQFNTCTNGLEDLLRYADRNAMAHGI
                     EVRLPFLNHQLVEFVLNLPENYKIREGFTKYVLRTAMSNQLPQNIVWRKDKIGFEPPQ
                     KLWMENKKMEELLMQAKFNLAKAGILKKQVIDLKLTNTNAHEVNNYNWWFLCMGNLIG
                     "
BASE COUNT         2031 a          725 c          912 g         1833 t
ORIGIN      
        1 caaaaaaatg aagagttttt tatttttata gtaattaaaa tgaataaata atggcaaata
       61 aaccggtaaa ggcattaagc actattgcag ctacagagcc tttaaaagtg gtaaatattg
      121 aaagaagaac tttaacagat aaagatattg aaattgaaat tttatattgt ggcatttgtc
      181 attcagattt acatgttgca caaaatgatt ggggtggctc tcattatcca ttagtaccag
      241 gacacgaaat agtaggtaaa gtaattggcg taggtaatag cactacaaaa tttaaaatta
      301 atgatatagt aggtgttgga tgtattgtag atagttgcca acattgtact tattgccaac
      361 aaggccatga gcaattttgc gaagaaggtt ggacagtagt ttttaattca aaagataaaa
      421 aacatggagg ctttacttat ggtggttttt caaccaatat aattgtgaat gaagattatg
      481 tagtacacat acctgctggc ttaaatttac ccaatgcagc acctatactt tgtgcaggca
      541 taactgttta ttcaccttta aaacattgga aagttgatgc taataaaact gtaggaatta
      601 ttggaatggg aggcttaggg cacatggcca taaaaatagc aaaagcaatg ggggcttatg
      661 ttacagtatt taccactaca ccaacaaaag ccaacgatgc gttacaactt ggtgcagata
      721 aagtaatcat ttcaaccaat gaacaagaaa tgaaacattg caccaaacaa gatattattt
      781 taaatactgt ttctgcaaaa cataatataa acgagtatat taatttacta aatgtagatg
      841 gtactattat tatggttggc atacctgcac aacctttaga gattggtgct tataatgtta
      901 ttaaaggcag aaaaagtttt tcgggttcta atattggagg cattgccgaa acacaagaag
      961 tattgaactt ttgtgcaaaa cataatatta cagccgatat tgaattgata aatcctgatt
     1021 atataaatac tgcttttgaa aggctaagta aaggcgatgt aaagtacagg tttgtgttag
     1081 atatgcaaaa aatataagtt cttgctatgt ttaaatactt tttatttata ctgttgttag
     1141 ggttttatac tagaactaaa gcacaaaaga tagaagctat ttattttaat ttatataccg
     1201 atagcttaaa aaaagaagta cataattaca ttaatataga aggaaaattg gctaacggaa
     1261 aatttattcc tctcgattct agtcacatta aattatcatg caatactggt aattggtatg
     1321 gaaataattt agtaatagat agtagttact taaaagactc tgttgtaatt acagcacaac
     1381 ttaaacaaaa cctacacctt caaaaacaaa taacagtttt tataaaaaaa ataaatgagg
     1441 tggctaacct taaaacagaa gaagaggtat tgcaaagcct tcaaccaacc aacaacaaaa
     1501 ataaaaagag aaaaaataaa cgatagttgt taaacagcat caaaccatac ttttttagta
     1561 tcattaaagc ttgcattata attaataaaa agttcttcac cagcttcaat ttgcctaact
     1621 gtaaccacac tcattgtttc attttcaaaa tccatttcat atacacaatt agctaagtag
     1681 ttatggttgt acatagatat aaaacccaat ccaatagcag ctttcatatt gttttctccc
     1741 cattcaaaaa tattattata caatttagtt tgttccacta ttgccctttc tttttcattt
     1801 aaaacaatta ctggagaaat ttctattgtt gttttacttg gaataatttg tgcagtaaat
     1861 acacctttac ctttgttatc actttctgaa ataaacaaaa caggaagtat catatttaaa
     1921 ataaattata acgaaagtaa tgtataaaat taaacaacat tgtaaattgc actcatgagt
     1981 attgaaatag aacaaattaa tttacaagaa cagtttctag aaatcattgc tacacaagat
     2041 aaaattttaa taaaagagtt cttagacaat caaaatatta gcgatgtagc cgatttaatt
     2101 tatcataata ctaacgatga ggctaccatt gttgccaata tggcaattaa tagagcagcc
     2161 aaagtattta aaattcttga ttttgcaacc caaaaacaaa tcattaataa attaccaagt
     2221 aataaaactg cagaattatt aaatgaatta cctgctgatg atagaacaga tttttttgaa
     2281 gagttaccta aaacagttat cagagattta attaaattgt tagatgctga tgaaagaaaa
     2341 attaccttat ccttattagg atatccagaa gatagtgtag gcaggttaat gacacctgac
     2401 tatgtttttg tatatactca taataccata gaagaagtac ttgaaaccat tagaaaatat
     2461 gcaaaaaata gcgaaactat tgatgtaatt tatattgtaa acgaacaaga cgaactaatt
     2521 gatgatataa gaattagaga tgtaatttta gcatcgccag agaaaaaaat ggaagaatta
     2581 attgatggca gatacgtagc acttcatgta aacgatgatc aggaacatgc cagccaaatt
     2641 tttaaaatga ataatagggt agcattgcct gttattgatg ataacaataa gctattaggt
     2701 attgtaacca ttgatgatat gctttgggta gcaaatgaag aatttagtga agacatgcaa
     2761 aaaatgggag gtaccgaagc attaaatgaa ccctatttag atatttctat ttttaaactt
     2821 tttagaaaaa gagtactatg gttggttatt ttatttttag gcgaattact tactgcctcg
     2881 gtaatgtttt attttgaatc ggaaattgca aaggcagttt ttttagcaac attcattccg
     2941 ttaattatta gtagtggtgg taatagtggc tcacaggctt ctacattaat cattcaagca
     3001 atggcagttg gcgaaattaa aattgcagat tggtggcgtg ttttaagaag agaaatttta
     3061 agtggtttaa tgcttggtac tgttttagga atgataggtt ttataagaat aatgatttgg
     3121 cacgaatttt ttccaaatat ttatggcgac cattttattt tagtaggctt tgtaatattt
     3181 tttagtttaa ttggtgttgt attatggggc acattaagtg gtagtatgtt accattggtt
     3241 ttaaaaaaaa tgggtgccga ccctgcagtt tcatcagcac catttgtggc tactttggtg
     3301 gatgttacag gtttaataat ttattttagt tttgcttact tgtttttaca aggcctaatt
     3361 ttataaagtt ttttctttaa tagttaacat ttattattcg tatccttgta acttaaaatg
     3421 taaccttcat gtgtgccatt gcaggcatta tgtcttttaa aaataatttg gttgaattaa
     3481 gcaatttgca aaaaatggcc aatattatgc agcatcgtgg aaatgatggg gaaggttttt
     3541 ggcttaataa ttcttctaca attggttttg cccacaaacg gttatctata atagatttaa
     3601 gcaaaaatgc agcacagcca atgcactatt tacagcatga agctaccaat aaatacactt
     3661 taatttttaa tggagaaatt tataattacg ttgaactaaa aaaggatctt attacaaaag
     3721 ggtatcaatt ttttacccag agcgatactg aagttttatt ggctttatac gattgttacc
     3781 aagaaaattg tttacaatat ttagatggaa tgtttgcatt tgctttatgg aataatgcta
     3841 cacaacaatt atggattgca agagatagat ttggcgaaaa acctttgtat tacaattgta
     3901 gcgatgagca atttttattt gcaagcgaaa tgaaagcttt atgggctgtc ggtgtagata
     3961 aaacaatcaa taatgcaagg ttgctaaact acctttctgt tggtatggtt agcaatccat
     4021 caaatgcaga ggaaactttt tataactcca ttaaaaaact accagcagca cattatttaa
     4081 taattaatta caatacaaaa gaaatttcta caaaaagata ttggcaggca aatttacatg
     4141 aagaaaaaaa tctattaaag gaagaagaag ttgtagagaa attctcttac ctgtttgcac
     4201 aatctattaa aagaaggtta agaagtgatg ttgctgtatc taccagttta agtggtggtt
     4261 tagatagctc ctctgttata gcaactatta ctgcattaaa taataaaaat aataatgaaa
     4321 agccaattag tttttctgct atttttcctg gttttgccaa agatgaatct gcttttatta
     4381 acgaggtagt taattatttt aatcttaaaa attatacaat taaccccaca gcagaaggtt
     4441 ttgtagcaga ttttgaaaaa ttactttttc atcaagagga accttttcag agtagtagta
     4501 tttatactca atttaaagta tatgaattag caaagttgca taatattaaa gttgtattag
     4561 atgggcaagg agcagatgaa attttagcag gttataccaa atattatttt tggtattggc
     4621 aatcgttact tagcaatttt aaatttagta ccttaaaaaa agaaaagatt gctgccaaaa
     4681 aaaataatca gcaaattgat tggaatagta agaatattgc agcagctttt ttcccaaatt
     4741 tggcagcaag aaaattaact tcaaacatta aaaataagat cttatttaat ccttatattg
     4801 aaaaagattt tataaatgct aacttcaacg agcaaagtgt acaaaagcct accattaaaa
     4861 acttaaatca aattttacag tttaatactt gtaccaatgg gttagaagat ttgttgagat
     4921 acgctgatag aaatgcaatg gctcatggta ttgaagtaag gttgcctttt ttaaaccatc
     4981 aattagttga gtttgtttta aacttacccg aaaattataa aataagagaa gggtttacta
     5041 aatatgtatt acgaactgca atgagcaatc aattaccaca aaatattgtt tggagaaagg
     5101 ataaaattgg atttgagcca cctcaaaagt tatggatgga gaataaaaaa atggaagaat
     5161 tattgatgca agcaaaattt aatttagcaa aagcaggcat attaaaaaaa caagtaattg
     5221 atttaaagct aactaatacc aatgcccatg aagtcaataa ttacaattgg tggtttttgt
     5281 gtatgggaaa tttgattggc taatatttat catttatggg tatgatttca gtcacttatt
     5341 tagcaactta tttataggtt ctttgcagcc ctaatatttt ctgtttatta tgtcacatac
     5401 ttttcatata cctgttttag gtacagctta cagtattgaa acacctataa aagttgcaca
     5461 tttaggcata tcatctgtta tatctattgt agatgatatt t
//
LOCUS       DAOQPK010000150         5479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_9013_length_5479_cov_4.522861, whole genome shotgun sequence.
ACCESSION   DAOQPK010000150 DAOQPK010000000
VERSION     DAOQPK010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9013_length_5479_cov_4.522861"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(288..>5479)
                     /locus_tag="PLU36_07680"
     CDS             complement(288..>5479)
                     /locus_tag="PLU36_07680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ46667.1"
                     /translation="GGARSSFSYTIPCSTPTALNAGGVTNNSANISFTGAGTAFIVEY
                     GATGFTPGTDANPGVGGTIVTGGSSPISLTGLSGNTAYDVYVRQDCTISGNGYSANST
                     KTTFTTLCGLISTYPFTEGFNAASLPACWSAYEGAQGASEHWQPVTADATHGAGTSAE
                     GSHFLLMNYYDAVLSYNPYYLKSIPFDLGATAKKVKFDIWMGKNSGTDNLKFEISTNN
                     GVSWTTLRTYSANPVNYSSNTPWDSKTIDLTAYTNQTVLFRLNATSNYGSGYCNIGFD
                     NFIIEAIPACNEPVDLIANSITQNTASISFTGAGSAFIVEYGPVGFTPGTDSTAGVGG
                     TLITGSASPIAISGLTGNTSYDVYVRQDCTGGGNGYSTNSSKATFTTLCGINSTYPYT
                     EGFNTPGVIPACWSVYEGVQGSGVQWQSGALTGYSQYDIRYPAEGAGFMFFYAYYYNP
                     NNAPYYLQSPIFNLGSSSKRARFALWMGAGVEADALKFQISTDSGSTWTSLASYSANP
                     SHNSPNAPWDSKTVDLSAYTNQSVLFRLSVTSGTILNYRFIGFDNFIIEDKPLQAPQV
                     VSVDSITKTTASVSFTEVGCTYIVEYGLAGFTPGTDSTAGVGGTIITTNSSPIAITGL
                     TANTAYDVYVRRDCSATGRGFSINSNKVSFTTKCNTVTDFSENFDASLPQCWAQVGSN
                     SVDVSNNRLRMMSYNSKAVVRMPEVSNADAGTHRLKFSTKKSGGQTGNIQVGYLTNQD
                     DPNTFVQLGNDYIPTKSSNSSDYDNFILSPVITPPGITTLAFRESTGNNGYIILIDNV
                     KYETIPNCLEATDIVANTITNNTAIIHFTGTGSAFVVEYGATGFTPGTNNNAAVGGTI
                     VTGGNSPIAITGLTANTTYDVYVRQNCTNSGNGYSANSIKATFTTLCDAANVPYILDI
                     PFVDQYTLPNCTYNQTLFQGTGPDRDSRWLIETPPSGNALYYQYYEDSVNTWFYTNGI
                     NLTAGSNYIINYMYAKQSGAIQKLKIAYGILPNNTAMITQLADHSITANFGYSSNSVS
                     FTPQVTGVYYFGFNLYTGSNTGGTSNKVRIKNISIDEVGQNKWTGTTDTDWNKGSNWS
                     LGTMPTPSSNVVIPNTINKPVIGVNFPLIREAKNITIQTGSNLTITNESSLLVYGNIA
                     NNGTIIADSGTIAFVGTTLQTFNTGNTDIAIKGLGMDNAVGLTLSGTGKLNVTSELYT
                     YRQGVFTTNNLLVLKSNENRTAILQSMFSLPTFVGNVTVERYIPAKASRKWSFLASPI
                     NQTLQDSWQQQIHITGAGTGGTVCPALTSHSNGFDATSTNAPSAYTYDASQTTGSRWQ
                     AVTNTNATNIGQGKAFRVNVRGSRSLGCDLLNGVNMVPNAVTLSSSGTLSTANKNFGT
                     FSITYPNNGINNWVLIGNPYPANVSFSDLLSDLGNSNAIDSVYAVYIPSNNAGVYSYW
                     SNVDGVFTGGTGYNNDNGNLIASGQAVFVKSKNVGNITLNFNEDLKYYTFSEEDLGYF
                     RNNRRTINEKVKVSYGTIDKKIDEIVVRYDNNVTISNTALGKLDIPSMNYGTYITSLK
                     GSTHTAVQTRSLSTLSTDEVWLNIGATQSGNYTFNFSDYENFTGADIYLVDHLAKITQ
                     DVKANPDYVFSVDVNNAATKGSNRFSIVFTKRIVEPSIVNNNIKMYPNPANKQVTLLL
                     PQIADINYNIKVTDMAGKIILQQSRAGGDKQLNIEKLSTGTYIVEIIDSKGNRTTEKL
                     IKN"
BASE COUNT         1591 a         1034 c          931 g         1923 t
ORIGIN      
        1 gcttcgctca gaatgacata aataatgggt gctcagtaga ttcttcgctt cgctcagaat
       61 gacataaata atgggtgctc agaatgacat aaatgagaat ctgtttgtgt tcttcctatt
      121 gacatgttta gcaaagcaaa acaaaaagca atgtcatgtt gagcaaagcg aaacatctgc
      181 ttttgcttta gtacgttttt cagcagatcc ttcgcttcgc tcagaatgac ataaataatg
      241 ggagctcagt agattcttcg cttcgctcag aatgacattt ttatttttta gttcttaatc
      301 aatttttctg ttgttctatt tcctttacta tctataattt caacaatata tgtaccagta
      361 gatagttttt caatatttaa ttgtttatcc ccccctgctc tactttgttg taaaattatt
      421 ttacctgcca tatcggttac ttttatgttg taatttatat cggctatttg aggtaataat
      481 aaggttactt gtttgtttgc agggttagga tacattttaa tgttgttgtt aacaatactt
      541 ggttctacta ttctcttagt aaatactata ctaaatctgt ttgaaccttt ggtagctgca
      601 ttgtttacat ctacactaaa cacatagtca ggattggctt ttacatcttg tgttatttta
      661 gctaaatgat ctactaagta tatatcggct ccagtaaagt tttcataatc gctgaaattg
      721 aatgtatagt tgccactttg tgttgctcca atgtttaacc atacttcatc ggtacttaaa
      781 gtacttaaac ttctggtttg tacggctgtg tgggtgctgc cttttaagct ggttatataa
      841 gtaccatagt tcatacttgg tatatccagt ttgcctaatg ctgtattgct tattgtaaca
      901 ttattatcat agcgtacaac tatttcgtct atttttttat ctattgtacc atagcttact
      961 tttacttttt cgtttatagt tcttctgttg ttacggaaat aacctaaatc ttcttcacta
     1021 aaagtataat attttaaatc ttcattaaag tttaaggtta tattaccaac attttttgat
     1081 tttacaaaaa cggcttgtcc gctggctatt aaatttccat tatcattatt atagccggtt
     1141 ccaccagtaa ataccccatc aacattgctc cagtagctat atacacctgc attattgctt
     1201 ggtatataca ctgcataaac actatcaatt gcattgctat ttcctaaatc tgataacaaa
     1261 tcactaaatg aaacattagc aggataaggg ttaccaatta atacccagtt atttatacca
     1321 ttgtttggat atgtaatgct aaatgtacca aagtttttat tggctgtact taatgtgcct
     1381 gatgagctta aagttactgc attaggtacc atgtttacac cattcaataa atcgcaacct
     1441 aagcttcttg atccacgtac gtttactcta aaagcttttc cttgtcctat attggtagca
     1501 ttggtattgg ttactgcttg ccaacgactg cctgtggttt gtgaggcatc gtaagtatag
     1561 gcacttggtg cattggtaga tgtagcatca aaaccattgc tgtgcgatgt taaagcaggg
     1621 caaaccgtac cacctgtacc tgctcctgta atgtgtattt gttgttgcca gctatcttgt
     1681 aaagtttggt ttattgggct ggctaagaaa ctccattttc tactggcttt ggctggtata
     1741 taacgttcta ctgtaacatt tccaacaaat gtaggcaatg aaaacattga ttgcaaaatt
     1801 gctgttctgt tttcatttga ttttaatacc aacaaattat tggttgtaaa tacaccttgc
     1861 ctataggtat ataattcact tgtaacattt aattttcctg ttccagaaag tgttaagcct
     1921 acagcattat ccatacctaa accttttatg gcaatatctg tatttcctgt attaaatgtt
     1981 tgcaatgtag tacctacaaa agctattgta ccagagtctg ctattatagt accattatta
     2041 gctatattgc cataaacaag cagtgaagat tcatttgtaa ttgttaagtt actacctgtt
     2101 tgtattgtta tattctttgc ctctcttata agtggaaagt ttacacctat taccggttta
     2161 ttgatggtgt tgggtataac tacattactg cttggagtag gcattgtacc taacgaccag
     2221 ttactgcctt tattccagtc tgtatcggtt gtacctgtcc atttattttg ccctacctca
     2281 tctatggata tgttttttat tctaacctta ttactagtac ctcctgtatt tgaacctgta
     2341 tataaattaa atccaaaata atatacacct gtaacctgcg gagtaaatga tacactatta
     2401 gacgaatatc caaaattggc agtaattgaa tgatctgcta actgggtaat cattgctgta
     2461 ttattgggta atataccata cgctattttt aatttctgta ttgcaccgct ctgcttcgca
     2521 tacatataat taatgatata attgctgccg gcagttaaat tgatgccatt ggtataaaac
     2581 caagtattaa ctgaatcctc ataatattga tagtaaagtg cattacctga tggtggtgtc
     2641 tctataagcc atctactatc cctatcaggt cctgttcctt gaaataaagt ttgattatat
     2701 gtacaattgg gtaatgtata ttgatctaca aaaggtatat ctaatatata tggcacattt
     2761 gcagcatcgc acaaagtggt aaaagttgct ttaatgctat tggcactata cccgttacca
     2821 ctgttggtac aattttgtct tacatataca tcgtaagtgg tattggcagt taaaccagtt
     2881 attgctattg gtgagtttcc gcctgttact attgttcctc caacagctgc attattattt
     2941 gttcccgggg taaaacctgt tgctccgtac tctactacaa atgcactacc agttcctgta
     3001 aaatgtataa tagcggtatt attagttatt gtattagcaa ctatatctgt tgcttccaaa
     3061 caattgggta ttgtttcata ttttacgtta tcaataagta tgatataacc gttatttcct
     3121 gtactttctc taaacgctaa agtagttata cctggtggag taataacagg gcttaaaata
     3181 aaattgtcat aatctgatga attagatgat ttagttggta tataatcatt tcctaattgc
     3241 acaaaagtat ttggatcatc ttgatttgtt aaatatccta cttgaatatt tccggtttgc
     3301 ccaccagatt tttttgtgct aaattttaac ctgtgtgttc ctgcatcggc attgcttacc
     3361 tctggcattc ttaccactgc cttgctatta taagacatca tgcgtaatcg attgttagaa
     3421 acatctacac tattactacc aacctgtgcc caacactgcg gtagtgaagc atcaaaattt
     3481 tcagaaaaat cggttactgt attacatttt gtagtaaaag aaactttgtt gctgtttata
     3541 ctaaagcctc ttccggttgc agagcaatct cttcttacat acacatcgta agcggtattg
     3601 gctgttaaac ctgtaatggc aatgggggag ctattagttg taataatagt acctcctaca
     3661 cctgcagtag aatcagtacc cggtgtaaag cctgcaagcc catactctac tatataagtg
     3721 caacccactt ctgtaaaaga tacagatgct gttgttttgg ttattgaatc tacacttact
     3781 acttgtggtg cttgcagtgg tttatcttca ataataaaat tatcaaaacc aataaaccta
     3841 taattaagta tagtaccaga agtaacactg agcctgaaca atactgattg gttggtgtat
     3901 gcagatagat ctactgtttt gctatcccat ggtgcatttg gagagttatg tgatggattt
     3961 gccgaatagg atgctaaaga tgtccatgta ctcccactat cagtactaat ttgaaacttt
     4021 agggcatctg cctcgactcc tgcacccatc caaagagcaa acctggctct tttagatgat
     4081 gaacctaagt taaatattgg agattgtaga taatatgggg cattatttgg gttataataa
     4141 taagcataaa aaaacataaa acctgccccc tcagcagggt agcgtatatc atattgagaa
     4201 tatccggtta atgcacccga ctgccattgg acacctgaac cttgtacacc ttcgtaaacg
     4261 ctccagcaag caggtattac acccggtgta ttaaagcctt cggtataagg gtatgtacta
     4321 ttaataccac acaatgtagt aaaggttgct ttagaactat tagtgctgta accatttcca
     4381 ccacctgtac aatcctgtct aacatataca tcgtaagatg tattacctgt taagccagat
     4441 attgcaattg gcgaagcact gcctgtaata agtgtgccac ccacacctgc tgtagaatca
     4501 gtacctggtg taaaaccaac aggtccatat tctactataa atgcactacc tgctcctgta
     4561 aaactaattg atgctgtatt ttgggttatt gaatttgcaa ttaaatcaac tggctcgtta
     4621 catgccggta tcgcttctat tataaaattg tcaaaaccta tattacaata accgctacca
     4681 taattagaag tagcattgag cctgaacaat actgtttggt tggtgtatgc agttaaatct
     4741 attgttttgc tatcccatgg tgtatttgaa ctgtagttta caggattagc agaataggtt
     4801 ctcaaagtcg tccagctaac accattattg gtgctaattt caaatttcaa attgtctgta
     4861 ccactattct tccccatcca aatgtcaaat tttacttttt tggctgtagc tcctaaatca
     4921 aatggtatcg attttaaata gtaaggatta taagatagaa ctgcatcata ataattcatc
     4981 agtaaaaaat ggctaccttc tgcacttgtt cctgcaccat gtgtagcatc tgctgtaaca
     5041 ggctgccaat gttcagatgc tccttgtgct ccttcgtaag ctgaccaaca agcaggtaaa
     5101 cttgctgcat taaaaccttc ggtaaaaggg taagtactta ttaaaccaca aagtgtagta
     5161 aaagtagttt tggtgctgtt ggcactataa ccattaccac ttatggtaca atcctgtctt
     5221 acatatacat cataagcagt gttgccagat aaaccagtta agctaattgg tgagcttccg
     5281 cctgttacta ttgttccacc aacaccagga ttagcatcag ttcctggtgt aaaacctgtt
     5341 gctccatact ctacaataaa tgcagtacct gctcctgtaa aacttatgtt tgctgaatta
     5401 ttggtaacac cacctgcatt taatgcagtt ggtgttgagc aaggaatagt atatgaaaaa
     5461 gaacttctgg cacctccgg
//
LOCUS       DAOQPK010000151         5440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_9112_length_5440_cov_3.967688, whole genome shotgun sequence.
ACCESSION   DAOQPK010000151 DAOQPK010000000
VERSION     DAOQPK010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5440
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9112_length_5440_cov_3.967688"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>5440
                     /locus_tag="PLU36_07685"
     CDS             <1..>5440
                     /locus_tag="PLU36_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46668.1"
                     /translation="IASYNSVIEKNVITRPTRTNTATTVNGIVFSAQNNGPIISKNKI
                     YEVFKAMPTNTATFNGIHFNASSPSAGNDYVITNNVVAASEGNGNQNGILNSNTNSLQ
                     MMHNTISLDNITSTTTASITTRGYNQTGASSGIIFYNNIVSVTRTGIGPKYCIYATSN
                     ATVPFCDYNNYFITNASNHYIGFNGANRQTMLTWVTATSQDLASLNFNPVFSNSFVGD
                     YAPVNAAIDNKALAGLVPDDINNVVRSATTPDMGAYEFTAPICTVPPVAGPTKILPDS
                     TCQNTPVALNVTLGQFGSGQTFQWQRSNTLLGTYTNVGNPKATPDTTINADTTGYYRC
                     AITCGGVTVYSDSVKLKVVPSLPGGVYTINSALPTSTNSPLVPGDNFASFNATRIAMG
                     CGITGAVTFNTVANSGPYNEQLILDSIAGASVVNTVTYNGNGNTLAFSPTNTNERAVL
                     KLKGTDYVIFDSLTIDASAATTFGYGVQLINHADTNIIRKSTILANSSSTSTNFAGIV
                     INSAHNNSITLGNTNCDGNKFYNNSISGGYYGVTLVGHSTSQISDNQFTNNEIKDFYN
                     YGMYIAGTANTLIEKNIIHRSTRTSVSNGYGIYLTAAANDRATISKNKIYNFFGGAPT
                     STLAAYGIYHNNVGVTAGNENNVTNNLIYQLDANNGSAASYGIYNASSAGINYFNNTI
                     AIDNASSTSSGISTGFYQSGAAVGIQFINNLVSVTRGGTGTKYAIQLLTVANNVTANY
                     NTYYVNAAGPTNFIGRYGSSNQADIAGWKTASGGDANSLSEDPIYVDPSTGNYKPQAL
                     SMDNKGTNTVGITTDILDIVRSATTPDIGAYEFAPSPCPSPLVAGTATVTPNVGMCLE
                     TPISLNLTGNSALGSVTFQWQASPNGNTGSWINIGQVQFTPKFDTLSSVTNYYRCIIM
                     CTGGVSDTSAVVQVNLNAILPAGSYTINSALPTDWPSPNPGPNANFNSFTDAVNALKC
                     GIKGHTIFNVVQGTYNEQIRIPYVPGTSATNGTITFQSANGNPTSAEITFAATTAANN
                     YTVKLDSAQHIFFKRLTISATGTSFAKAVEFANTASFDSLTNCIINAPTINNTSTNFA
                     VVSATQLKGTNITIKGNTINNGSVGIYFTGSSSTNKTFGHVIDSNNISGFYQYGVYVM
                     NNSMIGLNKNTINIASPLFSTNYGLYVSSTDSTYSVSSNKININNTSGTTYGIWITGS
                     GGTLFNEVKVTNNDILANTGNTGIIYGLRVTNSPNLFVANNAISTNTSSSCYGIYVDN
                     SGETKYYNNSINTMATSTTNGYALYVSNTTATGISVMNNIISNKGGGRAMYVTNPSAI
                     NSNYNMLYTTGNVLVQRGTPSTSYPTLLSWFNASMQDRYSIGYNPAFTSNTDLRPDLN
                     NHEVWAMHGRGTQLTDNNIDHDGNTRSTTLIAGVPDLGAYEFYPIAQPTVLGAIPATP
                     APNTTQTFYYGTDTVMRITWKATAPSSIELQRYSGVVPQGLAAANLDSMYFYVQSTIP
                     GGGNYDYDAKLYYLDPWLGSIQSPSQLGIGRTSQSNAWIVGVNSRNELAKRMLYDNEA
                     SYLDRFTGLFNPYAPPVIPDRDSSNRGRHFYFLYAANQLNAGATQEMTYYISTFEEPA
                     NVTIKVNGTNWTRSFLVPPYTVKAPLPVDYLPKAGADVAFYNVAGITDRSVEIISDVP
                     VVAYAHGIGSTSSGGCMLLPVGVWGYEYKTLTITQDYGANSYSLYYVVADNDNTTVEV
                     TSVAGVPTQNTTPPIPAGTPTVITLNKGQVLQVLGTSQTQELSGSLVKSIPNASGECF
                     PIAVFSGSSRTAIDMPAGCSSGGDFSMQQ"
BASE COUNT         1818 a          930 c          923 g         1769 t
ORIGIN      
        1 tattgccagt tataattcag ttattgaaaa aaatgtgatt acacgtccaa ctcgtaccaa
       61 cactgcaacc acagtaaatg gtattgtgtt tagtgcacaa aacaatgggc ctataatctc
      121 aaaaaataaa atttatgaag tatttaaagc aatgccaact aatacagcaa ctttcaatgg
      181 cattcacttt aatgcttcta gcccttctgc aggtaatgat tatgtcatca ctaataatgt
      241 tgtagcagca agtgaaggta atggaaatca aaatggaata ttaaatagca ataccaatag
      301 tttacaaatg atgcataata ctatctcttt agataatatt acttctacta caactgcatc
      361 tataactaca agaggttaca accaaacagg agcatcttct ggaattatat tctacaataa
      421 tatagtttct gttacaagaa caggtatcgg ccctaaatat tgtatttatg ctacttctaa
      481 tgctacagtt cctttctgcg attacaataa ctattttatt actaatgctt ctaaccatta
      541 cattggattt aatggtgcaa acaggcaaac aatgcttaca tgggtaactg ctacttctca
      601 agatttagca agcttaaact ttaaccctgt gttcagtaat tcatttgttg gagattatgc
      661 acctgtaaat gctgcaattg ataataaagc attagcaggg ctagttcctg atgatattaa
      721 taatgtggta agaagtgcta ctacaccaga tatgggagct tatgaattta ctgctccaat
      781 ttgtactgta cctccagtag ctggtcctac aaaaatatta ccagatagta catgccaaaa
      841 cacaccagta gctttaaatg ttactttagg ccaatttggt agtgggcaaa ctttccaatg
      901 gcaacgttct aatactttat taggaactta taccaatgta ggtaatccaa aagcaacacc
      961 agatacaaca attaatgcag atacaacagg ttattaccgt tgtgcaataa cttgcggagg
     1021 cgtaacagtt tattctgatt cagttaaatt aaaagtagtt ccttctttac caggtggcgt
     1081 ttatacaatt aacagtgcat tacctacaag tactaattca cctcttgtac ctggcgataa
     1141 ctttgctagc tttaatgcaa ctagaattgc aatgggttgt ggtataactg gtgctgttac
     1201 ttttaatact gttgcaaatt caggtccata taatgaacaa ttaattttag atagtattgc
     1261 tggagcttcg gtagttaata cagtaactta taatggtaat ggtaatacct tagcattctc
     1321 accaaccaat acaaacgaac gtgctgtttt aaaattaaaa ggtactgatt atgttatttt
     1381 tgatagctta acaattgatg caagtgctgc aactactttt ggttatggtg ttcagttaat
     1441 taaccatgcc gatacaaata ttattcgtaa atcaacaatt ttagctaaca gcagttcaac
     1501 aagtacaaac tttgcaggta ttgtaattaa ttctgctcat aacaattcta ttactttagg
     1561 aaatacaaat tgtgacggta acaaatttta caataattct atttcaggtg gttattatgg
     1621 agtaacgcta gtaggacatt caactagtca aatatccgat aaccaattca ccaataacga
     1681 gattaaagat ttttacaatt atggtatgta tattgccgga actgcaaata cattaattga
     1741 gaaaaatata attcatcgtt ctaccagaac gtcagtttct aatggatatg gtatttattt
     1801 aactgctgct gcaaatgaca gagcaactat ttctaaaaat aaaatttata acttctttgg
     1861 aggtgctcct acaagcacat tggctgcata tggtatttat cataataatg taggggttac
     1921 agcaggtaat gaaaataatg taaccaataa cttaatttat caattagatg ccaataatgg
     1981 ttcggctgca tcttatggta tatataatgc aagttctgct ggtattaatt actttaataa
     2041 tactattgca attgataatg caagcagtac ttcttcaggt atatccacag ggttctatca
     2101 atcaggtgca gcagttggta ttcagttcat taataattta gtttcagtta cacgtggtgg
     2161 tacaggtaca aaatatgcta tacaactttt aactgttgct aataatgtta cagcaaatta
     2221 taatacttac tatgtaaatg cagcagggcc aactaatttt atagggcgtt atggctcttc
     2281 aaaccaagcg gatattgcag gatggaaaac tgcctcagga ggggatgcta attcattaag
     2341 tgaagaccct atttatgtag atccttcaac aggtaattac aaaccacagg ctttatctat
     2401 ggataataaa ggaaccaata ctgttggaat tactacagat atcttagata ttgtaagaag
     2461 tgcaacaaca cctgatattg gtgcgtatga atttgctcct tcaccttgtc catctccatt
     2521 ggttgctggt acagcaacag ttacacctaa tgtaggcatg tgtttagaaa cgcctatttc
     2581 tttaaactta acaggtaaca gtgcacttgg ttcagtaact tttcaatggc aagcttcacc
     2641 aaacggtaat acaggaagct ggataaatat tggtcaagta caatttacgc ctaagtttga
     2701 tacattaagt tctgttacta actactatcg ttgtataatt atgtgtacag gtggtgttag
     2761 cgatacttca gcagttgtac aagtaaactt aaatgctatt ttaccagctg gtagttatac
     2821 tattaacagt gcattaccta cagattggcc ttcaccaaac cctggaccaa atgcaaactt
     2881 taactcattt accgatgcag taaatgcatt aaaatgcggt attaaagggc atacaatatt
     2941 taatgtagtt caaggtactt ataatgaaca aataagaata ccatatgttc caggtacttc
     3001 tgctacaaat ggtactatta ctttccaatc tgcaaatggt aatccaacat ctgccgaaat
     3061 tacttttgct gctacaactg cagctaataa ctatacagtt aaattagaca gtgctcaaca
     3121 tattttcttc aaaagattaa caattagtgc aactggaact tcatttgcta aagctgttga
     3181 atttgctaat actgcttctt ttgatagctt aactaattgt attattaatg caccaaccat
     3241 aaataataca tcaactaact ttgcggtagt tagtgctaca caactaaaag gaaccaatat
     3301 taccattaaa ggtaatacca tcaataatgg ttctgtaggt atttatttta ctggttcaag
     3361 ttctaccaat aaaacatttg gtcatgttat agatagcaac aatatttcag gcttctatca
     3421 atacggtgta tatgtaatga acaattctat gataggttta aataaaaata ctatcaatat
     3481 tgcatctcca ttattttcta caaattatgg tttatatgta tcttccactg actctactta
     3541 tagtgtttct agcaataaaa taaatattaa taatacatca ggtactactt atggtatttg
     3601 gataactgga agtggaggaa ccctatttaa cgaagttaaa gtaacgaata atgatatatt
     3661 ggctaatact ggaaatactg gtattattta tggtttaaga gtaacaaatt caccaaactt
     3721 atttgttgct aataatgcta tttctaccaa tacctcttca tcctgttatg gtatttatgt
     3781 agataactct ggtgaaacga agtattacaa caactccatt aatacaatgg ctacttctac
     3841 aaccaatggt tatgcattat atgtatctaa tacaactgca acaggtattt cggtaatgaa
     3901 taatattatc tctaataaag gtggtggtag agcaatgtat gtaactaatc cttcggctat
     3961 taacagtaac tacaatatgt tatacactac tggtaatgta ttagttcaaa gaggcactcc
     4021 tagtacttcc tacccaactt tattgagttg gtttaatgca agtatgcaag atagatattc
     4081 tattggctat aatcctgcat ttacttcaaa tacagattta agacctgact taaataatca
     4141 tgaagtttgg gctatgcatg gtagaggtac tcagttaaca gataataaca tagatcatga
     4201 tggcaataca agatctacaa ctttaattgc tggtgttcca gatttaggtg cttacgaatt
     4261 ttatccaatt gcacaaccaa ctgttttagg tgcaattcct gcaacacctg caccaaatac
     4321 tacacaaact ttctattatg gtacagatac tgtgatgaga ataacttgga aagctacggc
     4381 tccttctagt attgaattac aacgttattc tggcgtggta ccacaaggat tggcagcagc
     4441 taatttagat tcaatgtatt tttatgtaca atctacaatt cctggtggtg gcaactacga
     4501 ttatgatgct aaactatatt atttagatcc ttggttaggc tccattcaat ctccttctca
     4561 attaggtatt ggtagaacaa gccaaagtaa tgcatggata gtaggagtga atagtagaaa
     4621 tgaattagct aaacgaatgc tttacgataa tgaagctagc tatttagata gatttactgg
     4681 attatttaat ccttatgcac ctcctgtgat accagacaga gatagctcta acagaggtcg
     4741 tcatttttac ttcttatatg cagcaaacca attaaatgct ggtgctacac aagaaatgac
     4801 ttactatatt agcacatttg aagaacctgc aaatgttacc atcaaagtaa atggaacaaa
     4861 ttggactaga agcttcttag taccacctta taccgtaaaa gcaccattac ctgttgacta
     4921 tttaccaaag gctggtgcag acgtggcgtt ttataatgta gctggtataa cagatagaag
     4981 tgtagaaata attagtgatg tgccagttgt ggcttatgca catggtattg gtagtacatc
     5041 atctggtggt tgtatgcttt tacctgtagg cgtatggggc tatgagtata aaacacttac
     5101 aataactcaa gattatggtg caaattccta ttcattatat tatgttgtag cagataatga
     5161 taatacaaca gttgaagtta ctagtgtagc tggtgtacca acccaaaata caacaccacc
     5221 tattccagca ggtactccta cagtaattac tttaaataaa ggccaagtat tacaggtttt
     5281 aggtacatct caaacacaag agctatctgg tagcttagta aaatctatac ctaatgcaag
     5341 tggagagtgt ttcccaattg ctgtatttag tggtagctct cgtactgcta ttgatatgcc
     5401 tgcaggttgt agctctggtg gagattttag tatgcagcaa
//
LOCUS       DAOQPK010000152         5436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_9117_length_5436_cov_4.375581, whole genome shotgun sequence.
ACCESSION   DAOQPK010000152 DAOQPK010000000
VERSION     DAOQPK010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9117_length_5436_cov_4.375581"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PLU36_07690"
     CDS             complement(<1..385)
                     /locus_tag="PLU36_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46669.1"
                     /translation="MGVVILDTDVIIDLYRNKREALKTLDYFKDKELTISQISYAEFL
                     GGTRLKHKIEARKFLHQFKVLQEKENQKLFALCMKHEFKKQRSDKRNSSLGDFLIAQD
                     TLKANTILVTHNIDDFKIFESIKLHE"
     gene            complement(376..528)
                     /locus_tag="PLU36_07695"
     CDS             complement(376..528)
                     /locus_tag="PLU36_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46670.1"
                     /translation="MEYRKTETKTKGKAVAKKTAAKKPTKKGSYEAVMQGVIRAKKNP
                     IQFVWE"
     gene            complement(596..901)
                     /locus_tag="PLU36_07700"
     CDS             complement(596..901)
                     /locus_tag="PLU36_07700"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HMZ46671.1"
                     /translation="MGNNTLLQKESNLHRSKTQSINYNITNAIFLHDYTLLVTFANGI
                     TKPIDFTKALEKYAQGYYQKYRQPRHFKAFKIENQNIVWGKDWDLIFPPENIFKEKF"
     gene            complement(838..1137)
                     /locus_tag="PLU36_07705"
     CDS             complement(838..1137)
                     /locus_tag="PLU36_07705"
                     /inference="COORDINATES: protein motif:HMM:NF025088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HMZ46672.1"
                     /translation="MPKIFKYFGYLIYIYAHDHLPVHIHAKIQDREGKFILLYNNGIL
                     TIEDQNIKGKPHLTAKEVTEMRKFVRVYANKIKEQWETIHYYKKKVTCIEVKRRV"
     gene            complement(1146..1802)
                     /locus_tag="PLU36_07710"
     CDS             complement(1146..1802)
                     /locus_tag="PLU36_07710"
                     /inference="COORDINATES: protein motif:HMM:NF017116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE family protein"
                     /protein_id="HMZ46673.1"
                     /translation="MWRLKNLNKKDANKVKELFSLPDIKGRKPYGKLETQGFRIANFM
                     ASTNDEDFLVDATGNRRFLCFKVEKINDQEYSKIKIDDIWAEAYHYYRENTNNYYVTP
                     TDIVELEENNKQFLHVTQEYEYCNLYFKKPAENDTYYLLPASIIRDYLRILTMCQIIK
                     DRNIGIALKQLNFEQVTHRFTGSSFGIKVWKVALINDKIDGKQIIEPYHAHNNNLFKP
                     "
     gene            complement(1995..3104)
                     /gene="lpxB"
                     /locus_tag="PLU36_07715"
     CDS             complement(1995..3104)
                     /gene="lpxB"
                     /locus_tag="PLU36_07715"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HMZ46674.1"
                     /translation="MKYFIIAGEASGDLHGSNLIKQLRLADDNAIIQCWGGNLMQNAG
                     ATLLKHYKELAFMGFIEVVKNLPTILKNIKFCKQQINSFKPDVVVLIDYPGFNLRIAE
                     WAKKQGYKIVYYISPQVWAWKAGRVKKMKQCIDKMICILPFEQTYYQKKWNWKVDYVG
                     HPLIEAINQFKANYTAPNNSSNKFIALLPGSRKQEIIKKLPIMLEVAKAFPDYKFVVA
                     KAPGQDDDFYNQFLPVQPNVEFFRNDTYNVLMQSKAALVTSGTATLETALFEVPEVVC
                     YKGSNISYQIAKRLINIKYISLVNLIMDKLVVTELIQNKLTVTNLTTELNSLLNNEQR
                     KQQLQKDYTELKQILSISQQPSALAAKIIVDFINL"
     gene            complement(3220..4743)
                     /locus_tag="PLU36_07720"
     CDS             complement(3220..4743)
                     /locus_tag="PLU36_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMZ46675.1"
                     /translation="MNFLHELQLQNHNNGSSTGTQWLPSDGQLIKSFSPVDGNEIGTV
                     NSTTNNVYKSIVTKAEQAFEEWRLWPAPKRGEIVRQIGNALREKKEPLGKLVSYEMGK
                     SLQEGLGEVQEMIDICDFAVGLSRQLYGLTIHSERPNHRMYEQWHPLGIVGIISAFNF
                     PVAVWSWNAALAWVCGNVCIWKPSEKTPLCSIAVQNIVSEVFQKNNVPEGVNCLIQGY
                     KEAGEWLSQDAKVALVSATGSTRMGKAVNAAVAQRLGKTILELGGNNAIIISKDADID
                     MAIIGSVFGAVGTAGQRCTTTRRLIIHESIYDTLKQKLINAYQQLKIGNPLDSKNHVG
                     PLIDKDAVTQYLSSIEQCKQQGGKFIVEGGVLTGEGFESGCYVKPCIAEANNNMAIVQ
                     HETFAPILYIMPYHKIEEAIAMQNQVPQGLSSAIMTLNVREAELFLSHKGSDCGIANV
                     NIGTSGAEIGGAFGGEKETGGGRESGSDAWKAYMRRQTNTINWGSSLPLAQGIEFNI"
     gene            complement(4801..>5436)
                     /locus_tag="PLU36_07725"
     CDS             complement(4801..>5436)
                     /locus_tag="PLU36_07725"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HMZ46676.1"
                     /translation="VKTIPEKGYNIYEITTNDYPYSNKKLYIDSRFVKLSKLKPTERV
                     TKLPNQQDILSRMKKLEGFPYMWGGNTPNGVIEMLNLYKPKGTINQTTKNLWILKGVD
                     CSGLIYQATDGFTPRNTSSLVNYGNAVDNIENLNASQIAKLLKPLDLIVWNGHVVVVY
                     NETTTIESTPDGGVKTTDLTKRLQIILKDKKPVNNWNNTSGKRFVVRRWIN"
BASE COUNT         1565 a          956 c          774 g         2141 t
ORIGIN      
        1 attcgtgcag ctttatactt tcaaatattt taaagtcatc aatattatgt gtaactaata
       61 ttgtatttgc ttttaaggta tcttgtgcaa ttaaaaaatc gcccaagctg ctatttcgtt
      121 tatcgcttct ttgtttttta aattcatgct tcatacacaa ggcaaaaagt ttttgatttt
      181 ctttctcctg caatacttta aactggtgca aaaattttct tgcttcaatt ttgtgtttta
      241 gtcttgtgcc gcctaaaaat tcggcataag atatttgaga aatagttagc tctttgtctt
      301 taaaatagtc aagcgttttt aatgcttcac gtttatttcg gtataaatct ataattacat
      361 ctgtatctaa aataactact cccatacaaa ttgaatagga tttttttttg ctctaattac
      421 accttgcatt acggcttcgt agctgccttt tttggtaggc tttttggcgg ctgttttttt
      481 tgcaacagcc ttacctttgg tttttgtttc tgtttttctg tattccataa aaagcgtttt
      541 tacaaatata aatttttttg ttggtgtaag aggtaagtgg aaaaggttat aagtcctaaa
      601 atttttcttt aaatatgttt tctggtggaa aaattaaatc ccagtctttt ccccaaacaa
      661 tattttggtt ttctatttta aaggctttaa aatggcgtgg ctgcctgtac ttttggtaat
      721 agccttgtgc atatttttct aatgctttgg taaaatctat gggctttgta ataccattgg
      781 caaaagttac caataaggta taatcatgca aaaaaatagc atttgtaata ttgtagttta
      841 tactctgcgt tttacttcta tgcaggttac tttctttttg taataatgta ttgtttccca
      901 ttgctctttt attttattgg catatacccg tacaaattta cgcatttcgg taacttcttt
      961 tgccgtaagg tgtggcttac cttttatatt ttggtcttct atcgttaaaa ttccattatt
     1021 gtacagtaat ataaattttc cttctctgtc ttgtattttg gcgtgtatgt gtacaggcaa
     1081 atggtcgtgt gcataaatgt aaatcagata accgaaatat ttaaaaattt tgggcatggt
     1141 aaattttatg gtttaaataa attgttgttg tgtgcatggt atggctctat aatttgttta
     1201 ccatctattt tatcgtttat taaagctact ttccaaactt ttataccaaa gctgctgcca
     1261 gtaaacctat gtgttacttg ttcaaaattt aactgtttta atgctatgcc aatatttcta
     1321 tctttaatta tttggcacat tgtaagtatt cttaaataat ctcttataat agatgcaggt
     1381 agtaaataat aagtatcgtt ttcggcaggc tttttaaaat ataggttgca atactcatac
     1441 tcttgcgtta catgaaggaa ttgtttatta ttttcttcca gctctacaat atcggtaggt
     1501 gtaacatagt aattatttgt attttctctg tagtagtggt aggcttctgc ccaaatatca
     1561 tctattttta ttttgctgta ttcctggtca tttattttct ctactttaaa acaaagaaaa
     1621 cgcctgttgc ctgtggcatc tactaaaaaa tcttcatcat tagtgcttgc cataaagttg
     1681 gctattctaa agccttgtgt ttctaacttg ccataaggtt tacgcccttt aatatctggc
     1741 aaactaaaca actcttttac tttgttagca tcttttttgt ttaggttttt taatctccac
     1801 actttttcct ttaaatttgt ttattatggt tttttttaaa caacttgcag aatacctttt
     1861 tattaaaaaa agagacccta atgagcctag aactcaatgg acaaagtaca tgcacggcat
     1921 gaacagaatt tctttattta tgtttttaat tgctttactg ataattactt ttaaactgtt
     1981 tatattacct ttttttaaag gttaataaaa tctacaataa tttttgcagc caatgcagag
     2041 ggttgttgag agatgcttaa aatttgcttt aactctgtgt aatctttttg taattgctgt
     2101 tttctttgtt cgttgtttag taaactattt aattcggtag taagatttgt aactgttaat
     2161 ttattttgaa ttaattcggt aacaactaat ttatccatta ttaaatttac caacgatata
     2221 tattttatat taattagcct ttttgctatt tggtagctaa tattacttcc tttataacaa
     2281 actacttctg gcacttcaaa caatgcagtt tctaaagttg ctgtaccact tgttactaat
     2341 gcagctttac tttgcattaa tacattataa gtatcgtttc taaaaaattc aacatttggt
     2401 tgaacaggta agaactgatt gtaaaaatca tcatcttgcc ctggggcttt agcaactaca
     2461 aatttataat caggaaaagc ttttgccact tctaacataa tgggtagttt ttttataatt
     2521 tcctgttttc tactacctgg taataatgca ataaatttat tagaagagtt gttaggtgct
     2581 gtataatttg ctttaaattg atttattgct tcaattaatg gatgccctac ataatctacc
     2641 ttccaattcc attttttttg gtagtaggtt tgctcaaatg gtaaaatgca aatcatttta
     2701 tcaatgcatt gtttcatttt tttcactcgt cctgccttcc atgcccaaac ttgtggagat
     2761 atgtaataaa caattttata tccttgtttt tttgcccatt ctgcaattct taaattaaaa
     2821 ccaggatagt ctattaatac taccacatct ggcttaaaac tatttatttg ctgtttacaa
     2881 aattttatat tttttaatat ggttggcaag ttttttacta cttcaataaa gcccataaaa
     2941 gccaactctt tataatgttt taaaagagta gcacctgcat tttgcataag gtttccgccc
     3001 caacattgta taatagcatt atcatctgca aggcgtaatt gtttaattaa attactacca
     3061 tgcaaatctc cacttgcttc tcctgctatt ataaaatatt tcatattact tgcaaagtac
     3121 aatgatgtaa ttcatatttt gaaagaatca gaaataaaaa aatcagccaa aataaatgca
     3181 acaatttaag atggctgatt gtacttaatg tttaatggtt tagatattaa attctatacc
     3241 ttgtgccaat ggtaaactgc taccccaatt aattgtattg gtttgcctac gcatatatgc
     3301 tttccatgca tcgctaccac tttctcttcc tccaccagtt tctttctctc ctccaaatgc
     3361 accaccaatt tctgcaccac ttgtgccaat gtttacgttt gcaataccac aatcgctacc
     3421 tttatgagat aagaataatt ctgcctctct cacattcaaa gtcataatag ctgaggatag
     3481 gccttgtggt acttgatttt gcattgcaat agcctcttct attttatgat atggcataat
     3541 atataatata ggagcaaaag tttcgtgttg aacaatggcc atattattat tggcttctgc
     3601 aatacaaggc ttaacgtaac aaccagattc aaacccttct cctgtaagta ctccaccctc
     3661 tacaataaat ttaccacctt gttgtttgca ttgttcaata gaggataaat attgtgtaac
     3721 tgcatcttta tctattagtg ggccaacatg atttttacta tctaaaggat taccaatttt
     3781 tagttgctga tatgcattaa ttaatttttg tttaagagta tcataaatgc tttcatgaat
     3841 tattaacctt cttgtagtag tacaacgttg acctgcagta ccaacagctc caaaaacact
     3901 accaattatt gccatatcaa tatctgcatc tttagaaata ataattgcat tattgccacc
     3961 taattctaaa atagtttttc ctaaacgttg tgctactgca gcatttacag cttttcccat
     4021 tcttgtacta cctgttgcac ttactaaggc aaccttagca tcctggctta gccactctcc
     4081 tgcttctttg tatccttgta ttaaacaatt tactccttct ggtacattat ttttttgaaa
     4141 aacttccgac acaatatttt gtactgcaat agaacaaagt ggtgtttttt cactaggctt
     4201 ccaaatacat acattaccac aaacccaagc taatgcagca ttccaactcc aaacagcaac
     4261 aggaaaatta aaagctgaaa taatacctac aatacctaat gggtgccatt gttcatacat
     4321 tctgtggtta ggtctttcgc tatgaatagt taaaccatac aactgacgag aaaggcctac
     4381 agcaaaatca catatatcaa tcatttcctg tacttcgcct aaaccttctt gtaagctttt
     4441 acccatttcg tagctcacta atttacccaa tggttctttc ttttcacgca gtgcgtttcc
     4501 tatttgcctt acaatttctc ctctttttgg ggcaggccac aaacgccact cttcaaaagc
     4561 ttgttctgct tttgttacaa tacttttata cacgttgttt gtggtactat ttaccgtacc
     4621 aatttcattt ccatctaccg gtgaaaagct ttttatgagt tgtccatcag agggtagcca
     4681 ttgtgtgcct gtagaactgc cattattatg attttgtaat tgtaattcgt gtaaaaaatt
     4741 catatcctaa aatttaatgt tgcaaagtaa aggaatgtat ggtatctaaa atttggttta
     4801 ttaatttatc catcttctca ctacaaatct ttttcctgat gtattattcc aattatttac
     4861 aggcttttta tcttttaaaa taatctgtaa cctttttgtt aagtcagttg tttttactcc
     4921 tccatctggg gtactttcta tagttgtagt ttcgttatac accaccacaa catggccatt
     4981 ccaaactatt aaatctaaag gctttaaaag tttagcaatt tgagaagcat ttaaattttc
     5041 aatattgtca actgcgtttc cataattaac taaagaagaa gtatttcttg gagtaaagcc
     5101 atctgttgcc tgataaatta aacctgagca atcaacacct tttaatatcc ataaattttt
     5161 ggtggtttga tttattgtgc ctttgggctt gtaaaggtta agcatttcta taaccccatt
     5221 aggtgtgttg cctccccaca tatacggaaa gccttccaac ttcttcattc tacttaatat
     5281 atcttgttga tttggcaatt ttgtaactct ttcagttggc tttagttttg aaagctttac
     5341 aaaacggcta tcaatatata attttttatt gctgtagggg taatcgtttg tggttatttc
     5401 atatatattg taaccctttt ctggtattgt ttttac
//
LOCUS       DAOQPK010000153         5409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_9182_length_5409_cov_5.131864, whole genome shotgun sequence.
ACCESSION   DAOQPK010000153 DAOQPK010000000
VERSION     DAOQPK010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9182_length_5409_cov_5.131864"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            360..2318
                     /locus_tag="PLU36_07730"
     CDS             360..2318
                     /locus_tag="PLU36_07730"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HMZ46677.1"
                     /translation="MNAPNPKFNPILFPLIVVLVFTFSIIGHYSYLAHEGHAFNLFGV
                     IYATICFFVMHHVDPVPHNTYILVAQYLAALLICTSIFSLVFAKVKKAYLVYRIGITY
                     RNHIVIFRLNNIGKTLAIELLQKGYKVIVVESDEQNPFMEAIKLNGGIVFTEKPTDYK
                     TLKMAAVSYSRVCILTSNRDEINIEIAGTLASYLLKYSGRKKGDPHKIMVHINNTSNE
                     NLLKDYFDIQNEDDHYDLETFNVYESAAKKIYDTYSPYKYIDVSDKESENAIAIIGFN
                     DVAESFIVENMILSHYPDKERIRIYLVDDHADEKFNYISYKYPFFQEFIDLVPIKLIN
                     GTFFGNFTWSKVHIENLSKIKVVYVFGQKDAEIINISTNFRQFLYTQTLSISQVPIIA
                     CLPEDTGVLDLLNSNRESGETLSQVFSNKLNINTYRLITNTCTAEGLIEESETTQRLS
                     KLINFFYAIKYEFAAILENKWNISNAVSITNLLEEELLGLPDKYEKLHEREIEKHLLT
                     LLSLKTNIPTSDLYDNFSIEKHWQHLSYRKKDSNKYVARQIATKIQMLQYIGCTPINK
                     QNITTFFPRLAPIEHIRWSAEKMVFNFKYGPYPTDKNEKYLLKEVLKIHDQLVSYEKL
                     NEFDQEKDLNLFLMIPVLSIIKTNNGTK"
     gene            2330..3832
                     /locus_tag="PLU36_07735"
     CDS             2330..3832
                     /locus_tag="PLU36_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HMZ46678.1"
                     /translation="MQFSKDIFISYAHIDDESLIASQRGWISEFHRALEIRLAQLLGR
                     RPNIWRDMSLQGNHIFDQQIVSQFANVAIMISILTPRYVKSDWCIREVNEFYNACEQN
                     IGFNIQNIARIFKVIKTPIKIESHPEKIQNVLGYEFFSTDSNTGRVKEFGQVFGQQSE
                     LAYWEKLDDLANDICSFLETLESRTPTSETTNSTNDNFTKNRKKKIYLAEGSYDTLEF
                     RDSIKRELLDLDYTLLPDKQLPLVAPVLNNEVYSFLQNADLSIHLIGSGYGVVPEGTQ
                     KSIVEIQNEIASTISATKSLPRIIWVPENNVANDERQQNFLKKLQAGNEGIAGADLIV
                     SSLEDLKSIITDKLEKIKEEKNKDITESISTTNSTNKTKVVYLICDTNDLEIIQPLED
                     VLYNCGYEVLLPIFDGEETQIREDHIENLKICDTAIVFFGNGNELWLRSKMRDFIKIN
                     GYGRPQPLTSKIVYLAAPDNATKQRFRSVEAEVINGIHNVPEEKLISLLK"
     gene            3915..5189
                     /locus_tag="PLU36_07740"
     CDS             3915..5189
                     /locus_tag="PLU36_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HMZ46679.1"
                     /translation="MAEIFIIIGLIIINGLFSMAEIALVSARKARLEAQANKGNEEAE
                     RALKLANKPDSFLSTVQIGITLIGILTGFFSGENIKNDVVIWLQKFEILQPHSDKLAT
                     ALVVVVITYFTLVLGELLPKRIGLSNPEVIAKMVATPMMFISKIAFPFIWLLNKSTNT
                     LSKLFNILPNNSQVTEDEIKAIINEGTEQGTIEEAEQEIIERVFRLGDRNITSLMTHR
                     SDIIWLDVHKTIGELKSKFEEGIFSVYPVCDGSIDNIKGSVTIKDLFISDSNTNISTL
                     VKQPLYVPENNTAYQVLEKFKETKIHHCYIVNEYGSLEGMITLNDILEAIVGDIPEIT
                     EDNYEIIEREDGSLLADAQIPFIDFLHKVGKEDWINDEEDFDFDTLAGFILHHTQEIP
                     TTGERLQWRDFEFEIIDMDKHRIDKILISYKK"
     gene            5229..>5409
                     /locus_tag="PLU36_07745"
     CDS             5229..>5409
                     /locus_tag="PLU36_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HMZ46680.1"
                     /translation="MAYILTVLNKTPNMGNNCFIAPNATIAGDVVMGNECSIWFNAVV
                     RGDVNYIKMGNKVNVQ"
BASE COUNT         2039 a          757 c          876 g         1737 t
ORIGIN      
        1 tggggtgatg aaattcttgg cagacatgcc ctcttgtctc gggggtgagg ataatggaat
       61 aaacggagcg taatgccgct gtttgtgcac gaatagcgac tggggtcgac caccagtttt
      121 ctcaatggca attgagattt ttttgctctc tggctaacta cctcttgaag gttcgaaccc
      181 ttcccctaca gcaacttaca tttaaaagcc tttacagcat tatcttgtaa aggctttttt
      241 attagaggta ttttagtaat ctattcttca taaatcagtt ataaaatatc ctatatttct
      301 taaaccatta tatttgttag taccaaaatt tttcaaggtt taattattac ccataaaata
      361 tgaatgctcc taatccaaag tttaatccca ttttatttcc tcttatagtg gtattagttt
      421 ttacattttc tatcattggt cattattctt acttggctca tgagggacat gcttttaact
      481 tatttggagt aatttatgct actatttgtt tttttgtaat gcaccatgtt gaccctgtgc
      541 ctcacaacac ttatatatta gttgctcaat atttagcagc actacttatt tgtacaagca
      601 ttttttcatt agtatttgca aaagttaaaa aagcatattt agtttatcgt attggtatta
      661 cttacagaaa tcatatagta atttttaggt taaataatat tggtaaaaca ttagcaatag
      721 aacttttgca aaaaggttat aaagtaattg tagtggaaag tgatgaacaa aatcctttta
      781 tggaagccat taaactaaat ggtggaattg tttttaccga aaaaccaaca gactataaaa
      841 cattaaaaat ggctgctgta agctattcaa gagtatgcat attaaccagc aacagagatg
      901 aaataaatat agaaatagca ggcactttag catcgtacct actaaaatat agtggcagaa
      961 aaaagggaga cccacacaaa ataatggttc atattaacaa cacttccaat gaaaatttat
     1021 taaaagatta ttttgatatt caaaacgaag atgaccatta tgatttagaa acttttaatg
     1081 tatatgaatc ggcagcaaaa aaaatttacg acacctattc tccatacaaa tacattgatg
     1141 tgtcagataa agaatcggaa aatgcaattg caataattgg atttaatgat gtggccgaat
     1201 cttttattgt tgaaaacatg attcttagcc attatccgga taaagaaaga attagaattt
     1261 atttagtaga tgatcatgcc gatgaaaaat ttaattatat cagttataaa tatcctttct
     1321 ttcaagaatt tatagactta gtgcctatta aattaattaa tggtactttt tttggcaact
     1381 tcacttggtc taaagtgcat attgaaaatt taagtaaaat aaaagtggtt tatgtttttg
     1441 ggcaaaaaga tgctgaaata ataaatatct ctaccaactt ccgccaattt ttatatactc
     1501 aaaccttatc catttctcaa gtacctatta ttgcatgttt gccagaagat acgggtgtgt
     1561 tggatttatt aaattccaac agagaatctg gcgaaacatt gagccaagtt ttcagcaata
     1621 aacttaatat taacacttat cgtttaataa ctaatacctg taccgccgag ggattaattg
     1681 aagaatctga aactacgcaa aggctttcta aactcatcaa ctttttttat gcaattaaat
     1741 atgaatttgc agctatttta gaaaataaat ggaatataag caatgctgta tctattacca
     1801 atttattaga agaagaattg cttggcttac cagataaata tgaaaaacta cacgaaagag
     1861 aaatagaaaa acatttatta actctacttt ctttaaaaac aaatatccca acttccgatt
     1921 tatatgataa tttttctatt gaaaaacatt ggcaacattt atcttacaga aaaaaagatt
     1981 cgaataaata tgttgcaaga caaatagcaa ctaaaattca aatgttacaa tacattggtt
     2041 gtacaccaat aaacaaacaa aatattacta cattttttcc acgccttgcc cctatagagc
     2101 atattagatg gagtgcagaa aaaatggttt ttaattttaa gtatgggcca tatccaacag
     2161 ataaaaacga aaaatattta ttaaaagaag ttttaaaaat tcacgaccaa ttagtttctt
     2221 atgaaaaact aaatgagttc gaccaggaaa aggatttgaa tttattttta atgattcctg
     2281 tattaagcat aattaaaaca aacaatggaa ctaaataagc cttaaaagca tgcaattttc
     2341 taaagacata tttattagtt atgctcatat tgatgatgag tctttaattg catcacaacg
     2401 tggctggata agtgaatttc acagagcatt agaaattagg cttgcacaac ttttaggcag
     2461 aagacctaat atttggcgag acatgtcatt acaaggcaat catatttttg accaacaaat
     2521 tgttagccaa tttgccaatg tggcaattat gatttctatt cttacaccaa gatatgtaaa
     2581 aagcgattgg tgtattagag aagtaaatga attttacaat gcttgtgagc aaaatattgg
     2641 ttttaatatt caaaatattg ccagaatttt taaagttatt aaaacaccaa ttaaaataga
     2701 atcgcaccca gaaaaaattc aaaatgtatt gggctatgaa ttttttagta ctgattctaa
     2761 tactggtaga gtaaaagaat ttggacaagt atttggtcaa caatctgagt tggcatattg
     2821 ggaaaaatta gatgatttag ctaacgatat atgttctttt ttagaaaccc tcgaaagcag
     2881 aactcccact tccgaaacaa ccaattcaac aaatgacaat tttacaaaaa atagaaaaaa
     2941 gaaaatttat ttagccgaag gtagctacga tactttagaa tttagagaca gtattaaacg
     3001 agaattatta gatttagatt acactctatt gccagataaa caattaccat tagtggctcc
     3061 tgtattaaat aatgaagttt acagtttttt acaaaatgct gatttaagca ttcatttaat
     3121 aggctctggg tatggagttg tgccagaagg tacacaaaaa tcaattgttg aaatacaaaa
     3181 cgaaatagcc tccacaataa gtgcaactaa atcattacct cgtataattt gggtacctga
     3241 aaacaatgtt gcaaacgatg aaaggcagca aaatttctta aaaaaattac aagcaggtaa
     3301 tgaaggtatt gctggtgcag atttaattgt aagtagttta gaagatttaa aaagtattat
     3361 taccgacaag cttgaaaaaa ttaaagaaga aaaaaataaa gatattactg aaagtatctc
     3421 aacaaccaat agtaccaaca aaacaaaagt agtgtactta atttgcgaca cgaacgattt
     3481 agagataata caaccattag aagatgtatt gtacaactgt ggctacgaag ttttgctacc
     3541 catatttgat ggagaagaaa cacaaattag agaagaccat atagaaaatt taaagatttg
     3601 cgatactgca atagtttttt ttggtaatgg taatgagcta tggttaagaa gtaaaatgag
     3661 agattttatt aaaataaatg gttatggaag gcctcaacca cttacctcta aaattgttta
     3721 cttggcagca ccagataatg ccactaaaca aagatttaga tcggttgaag ccgaagttat
     3781 taatggcatt cacaacgtac cagaagaaaa attaattagt ctattaaaat aattaatttt
     3841 ttatcaaata catatactac agccagcctt tttacttaat caattaaaaa catatatctt
     3901 gcgttattat tatcatggct gaaattttta ttattattgg gttaattatt attaatgggc
     3961 tattctcaat ggccgaaatt gcattagttt cggcacgtaa agcaaggctt gaggcacaag
     4021 ccaataaagg caatgaagaa gcagaaagag ctttaaaatt agccaacaaa cccgattctt
     4081 ttctatcaac agtacaaatt ggcattacac taattggtat tttaactggt tttttttctg
     4141 gtgaaaacat aaaaaatgat gtggttattt ggcttcaaaa atttgagata ttacaaccac
     4201 atagcgataa gttggcaact gctttagtag tagtggttat tacttatttt actttagttt
     4261 taggtgagtt gttaccaaaa agaattgggt taagtaaccc agaagttata gcaaaaatgg
     4321 ttgctacacc aatgatgttt atatctaaaa ttgcatttcc ttttatttgg cttctaaata
     4381 aatctaccaa cactttatct aaacttttta atattttacc taacaactct caagtaacag
     4441 aagatgaaat aaaagcaatt attaatgagg gtacagagca aggtacaatt gaagaagcag
     4501 aacaagaaat tattgaaaga gtttttagat taggcgatag aaatataacc tcgttaatga
     4561 cgcatagaag cgatattatt tggttagatg tacataaaac tattggcgaa ctaaaaagta
     4621 aatttgaaga aggtattttt tccgtttatc ctgtttgcga tggaagtata gataatataa
     4681 aaggtagtgt aacaattaaa gatttgttta ttagcgatag caacactaat atttcaacct
     4741 tagtaaaaca acctttgtat gtgccagaaa ataatactgc ttaccaggta ttagaaaaat
     4801 ttaaagaaac aaaaattcat cattgttata tagttaacga atatggaagt ttagagggca
     4861 tgataacatt aaatgatatt ttagaagcaa ttgtaggaga tattcctgaa ataacagaag
     4921 acaattatga aataattgaa agagaagatg gaagcttatt ggcagatgct caaataccct
     4981 ttatagattt tttacataaa gtaggaaaag aagactggat aaatgatgag gaagattttg
     5041 attttgatac acttgcaggc tttatacttc atcatacaca agaaatacct actacaggag
     5101 agagattgca atggcgtgat tttgaatttg aaattataga tatggataaa catagaattg
     5161 ataaaatatt aatctcttac aaaaaataag caaagagtaa ctttattcta aaaatataaa
     5221 cctgttgtat ggcatatatt cttacagtac taaataaaac gcccaacatg ggcaataatt
     5281 gttttatagc acccaatgca accattgcag gagatgtagt aatgggaaat gagtgtagta
     5341 tatggtttaa tgcagttgta agaggcgatg tgaattatat aaaaatgggc aacaaagtaa
     5401 atgtgcaag
//
LOCUS       DAOQPK010000154         5345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_9344_length_5345_cov_3.851040, whole genome shotgun sequence.
ACCESSION   DAOQPK010000154 DAOQPK010000000
VERSION     DAOQPK010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9344_length_5345_cov_3.851040"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PLU36_07750"
     CDS             <1..443
                     /locus_tag="PLU36_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rRNA small subunit methyltransferase 1"
                     /protein_id="HMZ46681.1"
                     /translation="ALITDAGTPGISDPAFLLVRECIKENIVVECLPGATAFVPALVN
                     SGLPTNNFVFEGFLPLKKGRQTKLKQLATEDRTIVIYESPMRLVKTLEELAMYFGNDR
                     LCCVSRELTKMFEENKRGTLKENANYFAQKTVKGEIVITIAGAE"
     gene            complement(546..1736)
                     /locus_tag="PLU36_07755"
     CDS             complement(546..1736)
                     /locus_tag="PLU36_07755"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HMZ46682.1"
                     /translation="MKLSTLLNKFNEPETLKMAKLGRELRAQGKDVIDLSLGEPDFDT
                     PTHIKEAAIKAINDNWSHYTPVPGFLDLREAVCTKLKRDNNLDYLPENIVVSTGAKQS
                     LANAILALVDVNDEVIIPTPFWVTYSELVKIAGGKVVTISTTPEAKFKITPQQLEASI
                     TKNSKVFMFSSPCNPSGAVYSKQELAELVKVFEKYPNVFIISDEIYEFINYKDGHNSI
                     AQFSSIKDRVVVINGLSKGYAMTGWRLGYLAANTTIAKACEKLQGQFTSGTCSITQKA
                     AVVALTADLKPTFEMTEEFAKRKKRVLEIVKQIPGFKCTEPEGAFYIFPDVSYYYGKS
                     DGVTTISNASDLSMYILNTAYVSSVMGDAFGEPNCVRFSFANSLENIEKAWERIIAAL
                     NKLH"
     gene            complement(1960..2784)
                     /gene="kdsA"
                     /locus_tag="PLU36_07760"
     CDS             complement(1960..2784)
                     /gene="kdsA"
                     /locus_tag="PLU36_07760"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HMZ46683.1"
                     /translation="MNYSYLNNLFSNQTYHKQNFFLIAGPCVVESEEIVTEVAETVAN
                     VCKKLQIPYIFKASYRKANRTSATSFTGLGDELALDLIKKVGTTYNLPTLTDVHAHEE
                     CAMAANFVDVLQIPAFLCRQTDLLVAAAQTNKIVNVKKGQFLSGQSMQFAVDKIVKAG
                     NNKVMLTERGTTFGYQDLVVDYRNITWMKEHGTPVIMDCTHSLQQPNQTTGITGGNPA
                     LIGTIAKAAIATGADGLFIETHPNPSKAKSDGANMLPLHLLENLLEQLVKIRSVIQ"
     gene            2916..3860
                     /locus_tag="PLU36_07765"
     CDS             2916..3860
                     /locus_tag="PLU36_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMZ46684.1"
                     /translation="MTKEKILVIGASGQIGVELTLALRNLYGNNNVIASDLREQNPLL
                     EGTGPYVSLDVMNKEMLHVQVIRQNITQIYLLAAILSATGEKNPNLAWHLNMQGLLNV
                     LDIAREEHIHKVYWPSSIAVFGPTSPKQNCPQQTIIEPSTVYGISKYAGEFWCNYYHQ
                     RFGVDVRSLRYPGLISYKSAPGGGTTDYAVEIYLEALAEKKYECFLQEDTYLPMMYMP
                     DAIRATIELMEAPKSNINVRTSYNLSGMSFSPKEIALSIKQHIPEFEITYKPDYRQEI
                     ANSWPQSIDDSSANKDWGWKPQYNLQSMTEDMLTNLKS"
     gene            complement(4216..4719)
                     /locus_tag="PLU36_07770"
     CDS             complement(4216..4719)
                     /locus_tag="PLU36_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46685.1"
                     /translation="MIKKIIISFFLLLFINIVFAQQIDHPDDPPYLKVPYVPPFTLIL
                     SNKDKFKKDDLPKKHNIIMIYLNTDCGHCKTLVKHIVDSVQMAPNSFFVLSCYNKLKD
                     IKEFEETNNLKSYEQIVIGRDEKYFIPTFYRVKITPFVAVYNKKGDLIKAFSEGFSIA
                     ELQKILE"
     gene            4812..>5345
                     /gene="pth"
                     /locus_tag="PLU36_07775"
     CDS             4812..>5345
                     /gene="pth"
                     /locus_tag="PLU36_07775"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HMZ46686.1"
                     /translation="MNKFLIVGLGNIGNEYKHTRHNIGFDVIDAFVIKHGGFFKLDKL
                     AEVAEVKWKGRIFICIKPTTYMNLSGKAFRYWMEKEKVELENTLTIVDDLALPIEKIR
                     LRAIGSDAGHNGLRDIQFMLQTDKYPKLRIGIGNQFPKGMQAEFVLSKWLPTEQPIIN
                     KKIEKSIEIIENFATIGI"
BASE COUNT         1866 a          836 c          818 g         1825 t
ORIGIN      
        1 ttgccttaat tacagatgct ggcacaccag gtattagcga cccagcattt ttattagtta
       61 gagaatgtat taaagaaaat attgtggtag aatgtttgcc tggtgcaact gcttttgtgc
      121 ctgccttggt taatagtgga ttgccaacca ataactttgt ttttgaaggt tttttaccct
      181 taaaaaaagg aaggcaaacc aaacttaaac aattagcaac cgaagataga acaatagtaa
      241 tttacgaaag cccaatgcgt ttagtaaaaa ccttagaaga gcttgcaatg tactttggta
      301 atgataggct ttgttgtgta agtagagaat taacaaaaat gtttgaagaa aacaaacgtg
      361 gaacgctgaa agagaatgcc aattattttg ctcaaaaaac agtaaaaggt gaaattgtaa
      421 ttacaattgc tggtgcagag tagttgcact tcaaaattac ttcattacaa attgattgtt
      481 atttaaaaag cccttcttat acattaaaat aagaagggct tttattcttt ttcatttcaa
      541 aataattaat gcaatttatt taatgcagca ataattcttt cccatgcttt ttctatattt
      601 tccaagctat ttgcaaaaga aaaacgaaca caattaggtt ccccaaaagc atctcccatt
      661 acactactta cataagcagt atttaaaata tacatactta aatcagaagc attagaaatg
      721 gttgtaactc cgtctgattt accataataa taactcacat caggaaaaat ataaaaagct
      781 ccttctggtt cggtgcattt aaagcctggt atttgtttta caatttctaa cactctcttc
      841 ttcctttttg caaactcttc agtcatttca aaagtaggtt ttaaatctgc tgtaagtgct
      901 actactgctg ccttttgtgt aatagaacaa gtgccacttg taaattgtcc ttgtaatttt
      961 tcgcaagctt ttgcaatagt tgtatttgca gctaaatatc ctaaacgcca accagtcatc
     1021 gcatagcctt tacttaatcc atttatcacc actaccctat ctttaatgga actaaattgt
     1081 gcaatactat tatgtccatc tttgtaatta ataaattcat aaatctcgtc tgaaataata
     1141 aaaacatttg gatatttttc aaacactttc actaattcag ccaactcttg tttgctatat
     1201 acagcaccgc ttgggttaca aggcgatgaa aacataaaaa ccttagaatt ttttgtaata
     1261 gaagcttcta actgttgagg tgtaatttta aattttgctt cgggggttgt actaattgta
     1321 actactttac ctcctgcaat ttttactaat tcgctgtaag taacccaaaa aggcgttggt
     1381 ataattactt catcattcac atcaaccaat gctaaaattg catttgctaa gctttgtttt
     1441 gcacctgttg aaactacaat attctcaggt aagtaatcta agttattatc tctttttaat
     1501 ttagtacata ctgcctctct caaatctaaa aatcctggaa ctggtgtata gtggctccaa
     1561 ttatcattaa ttgctttaat agcagcttct ttaatatgcg ttggtgtatc aaaatcgggt
     1621 tctcctaaac ttaagtcaat tacatctttt ccttgtgctc ttaattcacg gccaagtttt
     1681 gccattttta aagtttcagg ttcattgaat ttgtttaata aggtagataa cttcataaca
     1741 aaaaaatttt aaatactatt gaaattatta ttaattttta tttgaattgt aaatataaat
     1801 gttgtgctaa attaaaatgt ctaattgggt taatattatg tttttagagg ttattaaaat
     1861 aaaaaacaaa agttcacttt tgcataagtt aattttatgt aaagggtgca ataccgttga
     1921 taacttatga gatgctcttg ttttcaagag catttttttt tattgtatta ctgatctaat
     1981 tttcactaac tgttctaata aattttctaa taaatgtaat ggcagcatat tggcaccatc
     2041 gctttttgct ttagatggat tagggtgtgt ttctataaac aagccatcag caccagttgc
     2101 tatggcagct ttggctattg tgccaattaa tgcaggattt ccgccagtaa ttcctgttgt
     2161 ttggttaggt tgttgaaggc tatgtgtaca atccataatt actggtgtac catgttcttt
     2221 catccaggta atatttctat agtcaacaac taaatcctga tacccaaatg tagttccacg
     2281 ttcagtaagc attaccttgt tgttgcctgc ttttacaatt ttatccactg caaattgcat
     2341 actttgccca cttaaaaact gtcctttttt tacatttaca attttatttg tttgggctgc
     2401 tgcaactaac aaatctgttt gcctacataa aaaggcaggt atctgcaaaa catccacaaa
     2461 attggcagcc attgcacatt cttcatgtgc atgtacatca gttaatgtgg gcaaattata
     2521 agttgtacca actttcttta ttaaatctaa agccaactca tctcctaatc ctgtaaaaga
     2581 tgttgcactt gttctattag cttttctgta gcttgcttta aaaatataag gtatttgtag
     2641 ttttttgcaa acatttgcca cagtttcggc aacctctgtt actatctctt cactttctac
     2701 aacacatggc ccggcaataa gaaaaaagtt ttgcttatgg taagtttgat tactaaataa
     2761 attgttcaaa taactataat tcatacaata aaaattacaa gttgcaaaca taggttaaat
     2821 agaaaataat aaaaggcttt taaatggtta aaactttata ttcctaagtt ccttttcttt
     2881 gcattttagc attttagaat tttataatcc ttattatgac caaagaaaaa attttagtaa
     2941 taggtgcaag tggacaaatt ggggttgaat taacccttgc tttaagaaat ctatatggta
     3001 ataacaatgt tattgcatct gacttaagag aacaaaatcc tttattggag ggtacaggac
     3061 cttatgtaag tttagatgta atgaacaaag aaatgttaca tgtacaagta ataaggcaaa
     3121 acatcactca aatttattta ttggcagcaa ttttaagtgc aacaggcgaa aaaaatccta
     3181 accttgcttg gcacttaaat atgcaaggtt tattaaatgt tttagatata gcaagagaag
     3241 agcatattca taaagtgtat tggccaagca gtatagcagt atttggccca acaagcccta
     3301 aacaaaattg ccctcagcaa acaattattg agccatccac tgtttatggt attagtaaat
     3361 atgctggtga gttttggtgt aattattatc atcaacgatt tggagtagat gtaagaagct
     3421 tgcgataccc aggattaata agttataaaa gtgcacctgg tggcggcact acagattatg
     3481 ctgtagaaat atatttagaa gcattggcag aaaaaaaata tgaatgtttt ttacaagaag
     3541 acacctattt accaatgatg tatatgccag atgcaataag agctaccatt gaattaatgg
     3601 aagcccctaa aagcaatatt aatgttagaa catcttacaa tttatcgggc atgagttttt
     3661 cgcccaaaga aattgcatta tctattaaac aacatatacc agagtttgaa ataacatata
     3721 aaccagatta taggcaagaa attgccaata gttggccaca gagcattgat gacagttctg
     3781 ctaataaaga ttggggctgg aaaccacagt acaatctgca aagcatgact gaggatatgt
     3841 tgactaattt aaaaagctaa cttttgtatc tgttaccgct ttggtaaagc tggcagaaag
     3901 aatacaaaaa agccaaaaca caacactaat aaggaataaa cccattcacc ttgtgttaag
     3961 taatgaattc ctaaaaatcc aaataatcct aagaaaccta atggccataa tcttttcata
     4021 gcaggtacgg ttttggttta aaaataaata gcctatatat aaaactttaa ttaagcagtt
     4081 tcttagcaat aaatttagga taaaccattg agtatcgcaa acaaaaaatg gcatacttat
     4141 tcacattttc tttttactgt gattagcaat tgattttgct tcttacattc ataaaaataa
     4201 atgcctaaaa catcattatt ctaaaatctt ttgcaattct gcaattgaga acccttctga
     4261 aaatgctttt atcaaatctc cttttttgtt ataaactgct acaaacggtg ttatcttaac
     4321 tctataaaaa gtaggaataa aatatttctc atctctacca ataactattt gttcatacga
     4381 ttttaaatta ttggtttctt caaattcttt aatatctttt aacttgttat agcaagacaa
     4441 aacaaaaaaa gaattaggtg ccatttgtac actatctaca atatgtttaa ccagggtttt
     4501 acaatgccca caatctgtat ttaaatatat cataataata ttatgctttt taggcaaatc
     4561 atctttctta aatttatctt tattagataa tattaaagta aatggtggta cgtatggaac
     4621 ttttaaataa ggtggatcat ctggatgatc tatttgctgg gcaaaaacta tgtttataaa
     4681 aagcaacaga aaaaatgaaa taataatttt tttaatcatt gtttaaaaat taaaaaatat
     4741 aagataaata atacccgttc aagctaaaac aaatatactt caaatttata ctgtaacttt
     4801 atttttgctg tatgaacaaa tttttaattg ttggtttagg aaatattgga aatgaatata
     4861 aacataccag gcataatatt ggttttgatg taatagatgc ttttgtaata aaacatggcg
     4921 gattttttaa gttagacaaa ttagcagaag tagcagaagt aaaatggaaa ggaagaattt
     4981 ttatttgtat taaacccact acctatatga acttaagtgg aaaagctttt agatattgga
     5041 tggaaaaaga aaaggttgaa ctagaaaata ccttaacaat tgtagatgat ttggccttgc
     5101 ctattgaaaa aattagatta agagctatag gtagcgatgc aggacataat ggactaagag
     5161 atatacagtt tatgctgcaa accgacaaat atccaaaact aagaattgga attggtaatc
     5221 agtttcctaa aggcatgcaa gcagagtttg ttttaagcaa atggctacca acagaacagc
     5281 caattatcaa taaaaaaata gaaaaatcta ttgaaatcat tgaaaatttt gctacaattg
     5341 gtata
//
LOCUS       DAOQPK010000155         5266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_9575_length_5266_cov_3.560353, whole genome shotgun sequence.
ACCESSION   DAOQPK010000155 DAOQPK010000000
VERSION     DAOQPK010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9575_length_5266_cov_3.560353"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..2299
                     /locus_tag="PLU36_07780"
                     /pseudo
     CDS             80..2299
                     /locus_tag="PLU36_07780"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
     gene            2397..3716
                     /locus_tag="PLU36_07785"
     CDS             2397..3716
                     /locus_tag="PLU36_07785"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HMZ46687.1"
                     /translation="MPTQDERVALASYEQLRGEDYHEIISLKPTFLARWALVLFAIIL
                     FLIVAASWFIYYPETITAKAKLNSTNAPKETIAHTDGKLTKITVKENEAVQAGQILAY
                     MESLANPNAVTAISQQADTIQYLMNVNKTNEIIYFFPDYTNQKFLNNLGELQTNYQTF
                     IQSFITFKDFLNNGFYLRKKIMLQTDLQNLQKLSAVLVHQRELLQADLALTHETFKAN
                     ETMAKEKIISSMDYRNEESKLIAKKLSLPQINASIINNESQQNDKRKEIAELDNQIAV
                     QKSIFMQALQTFKSEIQAWEYKYVIKAPVAGSVAFTGFYQENQEIKNGQSLFVVQPAN
                     TQYFVEAQILQYNFGKVKTGQQVLLKFPAYPYEQYGSVTGKIDFISTIPTDSGYLAKI
                     VLPDGLISNYKKPLQYHTGLKAQADIVTEDMRLLERFYYNIRKQLTR"
     gene            complement(3855..4805)
                     /locus_tag="PLU36_07790"
     CDS             complement(3855..4805)
                     /locus_tag="PLU36_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HMZ46688.1"
                     /translation="MKNFKAFIQLIRLPNLIFIAITQIIFLYCIEMPLFNEASVINNV
                     RGIYFVALVTASVFIAAAGNIINDYFDLNIDQINKPHKVVVEKIIKRRWVILWHILLS
                     LTGIVLGFYVDASTPVRFLGIANSACVVLLFFYSASLKKKFLIGNILISLLTSWVILV
                     ITYCETNHLFDVFRKDSSLQVDKLSRLTFLYAGFAFIISLIREVIKDMEDMEGDRKFG
                     CKTLPIVLGLNATKVFVAVWLIVLIAALVIIQFYVLQFGWVFWLSALYCVLFIITPLI
                     WILLKLFKAHTAQEFHKLSSVVKIVMFTGILSMLFFRLYA"
     gene            complement(4822..>5266)
                     /locus_tag="PLU36_07795"
     CDS             complement(4822..>5266)
                     /locus_tag="PLU36_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HMZ46689.1"
                     /translation="TLLPDGAMCRRMNIKDGYALQLAVKKQYNIVVISGGTSPESEMR
                     LRKLGITNIFMQVPDKATLLKEFLEEKKILSEQVLFMGDDVPDFNAMQIVGLPCCPTD
                     AAIEIKEIAHYISPLKGGEGCVRDVIEKVLKLQHNWKDDFFIQSK"
BASE COUNT         1905 a          899 c          830 g         1632 t
ORIGIN      
        1 tagcaaacaa ttagttgaac tatttggttt ttagtatatt ctcttgctat ttcttgtatt
       61 ccttttcaac aaatatttaa tgtcctttcc cttttataaa caactcaatg ctatggattg
      121 cggacctacc tgcttacgca tggtggcaaa atattatggc aggcatttta atacaggcgg
      181 cataagaaat atggcaggct ataccaaaga gggtgtaagt ttattgggca tcagccaaac
      241 agcagaaaac attggtttta gaacaagggc tgtacagcta acttataagc aactaacaga
      301 ggaagcaccc ttgccttgta ttttgcattg gaatcaaaac cattttgtgg tgcttactgt
      361 atataaaggc aacaactttt ttaaccaaca agccaaacta aatattgcag accccgccaa
      421 aggaatagta acatacacta aagaagaatt taccaagtat tgggtaagcc ataaagataa
      481 tgcaaacgaa agtataggta cagctttatt attagaacca acaactcaat tttacaaaca
      541 accccaccaa aaagaaacca aactgcattg gggtatcgta tttcaatatt tacgcaacag
      601 caaatggcaa ataacacagg tattcattgc attgctcatt gcttctttat tacagttaat
      661 atttcccttt cttacacaaa gcatggtgga tacaggtatt caaacacaca acctgcaatt
      721 cattaccatt gtattattag cccaattaat gttggtgttc agccaaactt ttgtggactt
      781 tattcgcagc aggttattac taaatatttc cgttgttcta aacttttctt tgctttcaga
      841 tttttggata aaacttacaa ggctgcccat cagctatttt gatacttacc atacaggcga
      901 tacattgcaa cgcatcggtg atatcaaacg tatataaagt tttttaacag gcagtgcatt
      961 aaacacgttc ttttctttat tcagctttct tgttttttct gtggtgctca ttttgtacaa
     1021 tgtgcagttg ttttttgtat ttgctgttgg cagcattttg tattttttat ggatacgttt
     1081 atttcttgct tacagaagaa aacttaatta tcaagcattt tatttatctg caaaagaaaa
     1141 taatgccaca ctgcaattgg tacaaggaat gcaggaaata aaactgcacg gagcagaaca
     1201 actgaaacgt tgggaatggg aaaatttaca ggcaggaatt tttaaactaa gttttaaaaa
     1261 tcttacatat agccaagtgc agcaatcggg tgcattactg attaatcagg gaaaaaatat
     1321 tgtcattact tttttggttg ccaatcttgt agtgcagggg cagcttacat tgggtgctat
     1381 gttggctgtg cagtatatta taggacaact gaacagcccc atagaacagt ttattggttt
     1441 tatgcaaatg gcacaagatg ccaaaataag tttggaaaga ataaatgaaa tacacgattt
     1501 gcaggatgaa gaaccaataa caacagaaga cttgcaacct gtaataattt taccacaaga
     1561 taaaacattg caattaaaca atttttcgtt tgcttatccc ggtgcaggca atgaacctgt
     1621 tttaaagaat attgatttaa tacttccaca aggaaaaaca acagccattg taggcacaag
     1681 cggcagtggt aaaacaactt tgctgaaact gctgttaaag ttttacaaca gctatgatgg
     1741 tgaaataaaa attggtgaaa cgaatttgaa atatataagt ccctcttttt ggagaaaaca
     1801 atgtggtgca gtattgcagg atggttatat ttttaacgac actattgcaa aaaacatagc
     1861 cgttggtgaa caacaaacag attatgaaaa gctgctgcac gcctgcaaaa cagccaacat
     1921 attgcagttt atagaaagcc tgcccaatgg ctttaatacc aagttgggaa cggatggaac
     1981 aggcataagt caggggcaaa agcaacgttt actgatagca agagccgttt ataaaaatcc
     2041 gcagtatttg ctttttgatg aagctaccaa tgctttagat gccaacaatg aaaaagctat
     2101 tgtagaaaat ttgcaggaat tttttatggg taaaacagta gtggttgtag cacatagatt
     2161 aagcacagta aaaaacgccg ataaaatagt agtgcttcat aatggacaaa tagcagaaca
     2221 aggtacgcac caagaacttt ctttactgaa aggaaaatat tttgaattgg taaaaaatca
     2281 attggagttg ggaggataaa agttaattgg ttaattaata atccaaaatg atattctttg
     2341 tgttctctgc ggctttgcgg taaaaaaact aaataatcat aaaatctccg ttctatatgc
     2401 ctacacaaga cgaaagagta gctttagcct cttacgaaca actaagaggc gaagactatc
     2461 atgaaattat ttcactcaag cctacgtttc ttgcacgttg ggcactggtt ttatttgcaa
     2521 ttattttatt cttaattgtt gcagcatctt ggtttattta ttatccagaa actattacag
     2581 caaaagcaaa actaaacagc accaatgcac ccaaagaaac cattgctcat acagacggaa
     2641 aactaacaaa aatcacagtt aaagaaaatg aagctgtaca ggcaggacaa atattggcat
     2701 acatggaaag ccttgccaat cctaatgctg ttactgcaat cagccaacag gcagatacta
     2761 ttcaatactt aatgaatgtc aataaaacca atgaaattat ttactttttt cccgattata
     2821 ccaatcaaaa atttttaaac aatttaggag aactgcaaac taattatcaa acttttatac
     2881 aatctttcat tacgtttaaa gattttttaa acaatggttt ttacctacgc aaaaaaatca
     2941 tgctgcaaac agatttgcaa aatttgcaaa aactaagtgc tgtacttgtt catcaaagag
     3001 agttgttaca ggcagacctt gcactaaccc atgaaacatt taaagccaac gaaacaatgg
     3061 caaaagaaaa aataatttct tcaatggatt acaggaatga agaaagcaaa ctgattgcca
     3121 aaaaattatc gttgccgcaa atcaatgctt ctatcattaa caatgaaagt cagcaaaatg
     3181 ataaacgaaa agaaatagca gaattagaca atcaaattgc tgtgcagaaa agtattttta
     3241 tgcaggcatt gcagacattt aaatccgaaa tacaagcatg ggagtataag tatgtaataa
     3301 aagcccctgt tgcaggcagc gttgcattta cagggtttta ccaagaaaat caggaaataa
     3361 aaaacggaca atctttattt gttgttcagc ctgccaatac acaatatttt gtagaagcac
     3421 aaatactgca atataatttt ggtaaagtaa aaacagggca gcaagtgttg ttaaaatttc
     3481 cggcttatcc ttacgaacag tacggctctg taacaggcaa aatagatttt ataagcacaa
     3541 tcccaacaga tagtggttat ttagctaaaa tagttttacc cgatggatta ataagtaatt
     3601 acaaaaagcc actgcaatac catacaggct taaaagcaca agcagatata gtaacagaag
     3661 atatgcgatt gttagaaagg ttttattata acattcgtaa acaacttact cgatagtata
     3721 atccttgctt cccatttact taactattga caatcaaaag ttgaaatttt aattatggag
     3781 attgttgatt ttaacattac ttgttgtgta ggttctatac aaatagaata atctataaaa
     3841 tttaatttat ctatctacgc atacaatcta aaaaaaagca tacttaaaat tccggtaaac
     3901 attactattt taactacact gcttagttta tgaaactctt gtgcagtatg ggctttaaag
     3961 agttttaata aaatccaaat taaaggagtt attataaata atacacaata taaagcactt
     4021 agccaaaata cccaaccaaa ttgcaacacg taaaattgta ttattaccaa tgctgcaatt
     4081 aatactatta accaaacagc aacaaacact tttgttgcat ttaaaccaag tacaattggt
     4141 agtgttttac aaccaaattt tctgtcgcct tccatatctt ccatatcttt tataacttct
     4201 ctaattaatg aaattataaa agcaaaacct gcatataaaa aagttagtct agaaagttta
     4261 tcaacttgta aactgctatc ttttctaaac acatcaaata aatgattggt ttcgcaatag
     4321 gttataacta aaataaccca tgatgttagt aaagaaatta gtatgttgcc aattaaaaat
     4381 ttttttttaa gagaggcaga gtaaaaaaac agtagtacaa cacaagcaga atttgcaatt
     4441 cctaaaaatc taacaggtgt tgaagcatct acataaaagc ctaatacaat gcctgttaaa
     4501 cttagtaaaa tatgccatag aataacccac cttcttttta ttattttttc tacaacaact
     4561 ttgtgtggtt tattaatttg gtcaatattc aaatcaaaat aatcgttaat gatgttacct
     4621 gcagcagcta taaaaactga tgctgtaacc aatgcaacaa aataaatacc tctaacatta
     4681 ttgattacac tggcttcatt aaacaaaggc atttctatac aatacaaaaa aataatttgt
     4741 gtaattgcaa taaatattaa attaggcaat cgtattagtt gtatgaatgc tttaaagttt
     4801 ttcaattaaa acaatttaaa tttatttaga ttgaataaag aaatcatcct tccagttgtg
     4861 ctgcaacttt aatacttttt ctattacatc tctcacacaa ccttctcctc cttttagtgg
     4921 agaaatataa tgtgctattt ctttaatttc aattgctgca tcagtaggac aacatggtaa
     4981 gccaacaatt tgcattgcat taaaatctgg cacatcatca cccataaata aaacctgttc
     5041 ggataatatc tttttttctt ctaaaaattc tttaagcaat gtagccttat ctggcacttg
     5101 cataaaaata tttgttatgc ctaattttct taatctcatt tccgactctg gtgaggtacc
     5161 accacttatt accacaatat tgtattgttt ttttacagct aattgtaaag cataaccatc
     5221 tttaatattc attcttctgc acatggcacc atcaggcaat aatgtt
//
LOCUS       DAOQPK010000156         5162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_9896_length_5162_cov_4.967887, whole genome shotgun sequence.
ACCESSION   DAOQPK010000156 DAOQPK010000000
VERSION     DAOQPK010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9896_length_5162_cov_4.967887"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1895)
                     /gene="secA"
                     /locus_tag="PLU36_07800"
     CDS             complement(<1..1895)
                     /gene="secA"
                     /locus_tag="PLU36_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216478.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; in some organisms, there are paralogous
                     proteins that have been found to be nonessential but do
                     function in secretion of a subset of exported proteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HMZ46690.1"
                     /translation="MIKFLSKLLGGNKSEKDVAAIQPYIIKINDFFKQYQLLTNDELR
                     NKTTEFKQRIKDYLTEIDTIIEDKKLTADNLPVQDVHSKDILYSEIDKLKKDRDKKIE
                     EVLEKLLPEAFAVVKETAFRFTNNTELKSTATNLDRDLSVQKDYVTIDGDQSIFKNTW
                     IAAGGQIIWNMVHYDVQLIGGSVLHQGKISEMATGEGKTLVSTLPAYLNALAGEGVHI
                     VTVNDYLAKRDSEWNGPIFEWLGLTVDCIDKHEPNTEDRRNAYLADITYGTNNEFGFD
                     YLRDNMVHNPDEMVQRKHHYAMVDEVDSVLIDDARTPLIISGPVGKDTSTEQFFELKP
                     RIERIVEAQKKSVNQFLIEAKKKIAEGNDDPKDGGLALFRAHRGLPKNTALIKFLSEP
                     GIRVKLQKTENYYLADQQKEMPKADAELYFYIDEKNNSVELTDKGIVLITRSGEDPNF
                     FILPDISVALAEIENNSNYTTDEKLHKKEVVITEYSIKADRIHTVQQLLKAYTLFEKD
                     DEYVVIEDQVKIVDEQTGRIMEGRRYSDGLHQAIEAKENVKIEATTQTYATVTLQNYF
                     RMYHKLAGMTGTAETEAAELWDIYKLDVVSIPTNKTLIREDEQDKVYKTKREKFGAVI
                     DEIVKQK"
     gene            complement(2167..3678)
                     /gene="gltX"
                     /locus_tag="PLU36_07805"
     CDS             complement(2167..3678)
                     /gene="gltX"
                     /locus_tag="PLU36_07805"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HMZ46691.1"
                     /translation="MNKKVRVRFAPSPTGGLHLGGVRTVLFNYLFAKQHNGEFVLRIE
                     DTDQTRFVEGAEQYIYDTLDWCGLVPDESPLHGGNFAPYKQSQRKDLYKQYALKLVED
                     GFAYYAFDTANELELKRNSIPNFQYNHITREGMKNSLVLDETTTKELLKSGLPYVIRM
                     KMPHNEDIRFNDMIRGEIHFNSSLVDDKVLLKADGMPTYHLAVVVDDYLMQISHAFRG
                     EEWLPSAPVHILLWKYLFGLNNMPQWAHLPLILKPDGNGKLSKRDGDRLGFPVFAKDW
                     YDPKTNETTVGFKERGFLPQAFLNMLVMLGWNDGTEQEIFTLQEMVEKFSIERVHKGG
                     AKFDYEKAKWFNHAWIMKLDINSLNLQVKNVLGSLTVGYSDSYINQVINLTKDRCTFI
                     ADFEQQTSYFFKAPINVDVAAIKPKWDNNKQHFFEEYLNWLNTNFDWSTSILEQEFKN
                     LASANGLKHGELMLPFRIMLVGGKFGPAVFDIANIIGKEQTITRIKHVLNLLA"
     gene            3741..4520
                     /locus_tag="PLU36_07810"
     CDS             3741..4520
                     /locus_tag="PLU36_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HMZ46692.1"
                     /translation="MNVRTMADMMANNETPEILFWIGCAGSFDQRAQKITKAFAAILD
                     KTGIKYAILGKEEACTGDPARRAGNEFLFQMMAYQNIQILNGYGIKKIVTTCPHCLNI
                     MKNEYPELGGNYEVIHHTTLLQQLINDGKIKLKEGGSFKGKKITYHDSCYLGRANNIY
                     EAPRKVLEALDAELVEMKRCGSKGLCCGAGGAQMFKEEEAGNKRINIERTEEALETKA
                     NFIASACPYCNTMMTDGVKLKEKEADVQVLDIAEMIANEME"
     gene            4540..4746
                     /locus_tag="PLU36_07815"
     CDS             4540..4746
                     /locus_tag="PLU36_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46693.1"
                     /translation="MKNFNVKKFIKATLILLPILLLVDIVYDMIFKELILKETFAMKN
                     LFFKIAASLVGAYFFATSKTKEEN"
     gene            4784..>5162
                     /locus_tag="PLU36_07820"
     CDS             4784..>5162
                     /locus_tag="PLU36_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46694.1"
                     /translation="MDISYSHLIPTNFSDNAKVWIYQSSRKFTINEALQIEAILDDFI
                     AQWQSHGIPVKGFATLFFGQFLVFMADDNVDVSGCSTSSSVDVVKQVEKIFNVEMFNR
                     QNLAFVVKDEVQLLPLAQLNYALS"
BASE COUNT         1673 a          864 c          730 g         1895 t
ORIGIN      
        1 tctttttgtt ttacaatttc atctattaca gcaccaaact tttctctttt tgttttatac
       61 actttatctt gctcatcttc tcttattaaa gttttattag ttggaattga aactacatct
      121 aatttataaa tatcccaaag ttcagcagct tcagtctctg cagtacctgt cattcctgct
      181 aatttatggt acattctaaa atagttctgt aaggttactg tggcgtatgt ttgagtagtt
      241 gcttcaattt tcacgttttc tttagcttca atagcctggt gcaatccatc actatatctt
      301 cttccttcca taatacgacc agtttgttca tcaactattt ttacttggtc ttcaattact
      361 acatattcat catctttttc aaataaagta tatgctttta gtaattgttg aacagtatgt
      421 attctatctg cctttataga atattcggta attacaactt cttttttatg taatttttca
      481 tcggttgtat aatttgaatt attctctatt tctgccaatg caacacttat atcaggaagt
      541 atgaagaaat taggatcttc accggaacga gttattagca caattccttt atcggtaagt
      601 tcaacactat tgtttttctc atcaatgtaa aaatataatt cagcatcggc tttaggcatt
      661 tctttttgct gatcggctaa ataataattt tctgtttttt gaagctttac tctaatacct
      721 ggttcactta aaaacttgat taaagctgta ttttttggta agcctctatg agccctgaat
      781 aatgctaaac caccatcttt ggggtcatca ttaccttctg caattttctt tttagcttca
      841 attaaaaatt ggtttacact ttttttctgg gcttctacta ttctttcaat tcttggttta
      901 agttcaaaga attgctccgt actagtatct tttcctactg ggccgctaat aattaaagga
      961 gttctagcat catctattaa aacactatcc acttcatcta ccatggcata atgatgttta
     1021 cgttgaacca tttcatctgg gttatgtacc atgttatctc taagataatc aaacccaaac
     1081 tcattattag taccataagt tatatctgct aaataggcat ttcttctatc ttctgtatta
     1141 ggctcatgtt tatctataca atcaactgtc aagccaagcc attcaaatat tggtccattc
     1201 cattcactat cacgttttgc taaataatcg tttacagtta caatatgcac cccttctcct
     1261 gctagagcat taagataagc aggtaatgtt gataccaatg ttttaccttc tcctgtagcc
     1321 atttcagata ttttaccttg atgaagtact gagccaccaa ttagctgcac atcataatgt
     1381 accatattcc atattatctg accacctgct gctatccaag tatttttaaa aatggattgg
     1441 tctccatcaa tggttacata atctttttga acacttaagt ctctatctaa attagttgca
     1501 gtagatttta gttctgtatt attagtaaac ctaaatgcag tttcttttac tacagcaaaa
     1561 gcttcgggta ataatttttc taaaacttct tctatctttt tatctctgtc tttcttaagt
     1621 ttatcaatct cgctatataa aatatctttt gaatgaacat cttgaacagg taaattatca
     1681 gcagttaatt ttttatcttc aatgatggta tcaatttcag ttaaataatc ttttattctt
     1741 tgcttaaatt cagtagtttt attacgcaat tcatcatttg taagtaactg gtattgctta
     1801 aagaagtcat taatttttat aatatagggt tgaatggctg caacatcttt ttcactttta
     1861 ttacctccta aaagttttga tagaaactta atcatattgg ttattattta tgcacttttc
     1921 ttctgtaata atctctttta tcctacttca attaatacta ataattgtgc aattaaattt
     1981 tagttattct tttatatttc ttgaaaaaac ttagtagaaa attttatgcc taatttattt
     2041 tacataaaaa tggttacatc taaaacaaat tattagccaa attggcacta atgcttttta
     2101 aggctgccaa agttaacaga ttttagaata ttgaaatgta ctaaaatgct aaaattttta
     2161 actagtttaa gccagtaaat ttaatacatg cttaattctt gttatagttt gttctttgcc
     2221 aataatattg gcaatatcaa aaactgcagg gccaaatttt ccacctacaa gcattattct
     2281 aaaaggcaac atcagttcgc catgttttaa accattggct gatgctaaat ttttaaactc
     2341 ttgttctaat atggatgtac tccaatcaaa gttggtattt aaccaattca aatattcctc
     2401 aaaaaaatgt tgtttattat tatcccattt tggtttaata gcagctacat caacatttat
     2461 aggggcttta aaaaaataag aagtttgttg ttcaaaatct gcaataaaag tgcatctatc
     2521 tttagttaaa ttaataactt gattaatata agaatctgaa tatcctactg ttaaacttcc
     2581 taaaacattt ttaacttgaa ggtttaaact attaatatct agtttcatta tccatgcatg
     2641 gttaaaccat ttggcttttt cataatcaaa ttttgcccct cctttatgaa cacgttcaat
     2701 agaaaatttt tctaccattt cttgtaacgt aaatatttct tgttctgtgc catcattcca
     2761 tcctaacata accaacatat ttaagaaggc ttgtggtaaa aaaccacgtt ctttaaagcc
     2821 aactgttgtt tcatttgttt tagggtcgta ccagtccttg gcaaatacag gaaatccaag
     2881 tctatcgcca tctctcttac ttaatttacc attaccatct ggttttaaaa ttaaaggtaa
     2941 atgagcccat tgaggcatat tgtttaatcc gaataaatac ttccaaagta aaatatgtac
     3001 tggagcactt ggtagccatt cttctcctct aaatgcatgg cttatttgca ttaaataatc
     3061 atctactaca acagctaaat gatacgttgg cattccatct gcttttaata aaactttatc
     3121 atctaccaac gaagagttaa aatgaatttc gcctcttatc atatcattaa aacggatgtc
     3181 ttcattgtgt ggcatcttca ttcttataac ataaggtagc ccagatttca gtaattcttt
     3241 ggttgtagtt tcatctaata ccaaagagtt tttcataccc tctctagtaa tatggttgta
     3301 ttggaaatta ggaatgctgt tacgttttaa ctcaagttcg ttagcagtat caaatgcata
     3361 ataagcaaaa ccatcctcca caagtttaag tgcatattgc ttatataaat cttttctttg
     3421 gctttgttta tatggtgcaa aattacctcc atgcaaagga ctttcatcag gcactaatcc
     3481 acaccaatct aaggtatcat aaatatattg ttcagcacct tctacaaatc ttgtttggtc
     3541 tgtatcttca attcttaata caaattcgcc attatgttgc ttagcaaata aataattaaa
     3601 caaaactgtt cttacaccac ctaaatgtaa gccacctgtt gggcttggag caaaacgaac
     3661 acgtactttt ttattcataa gtttgcaaag ataagccctt cgtttttttc cttattttcg
     3721 caacactaat ttgtttgttt atgaatgtta gaacaatggc agatatgatg gcaaataatg
     3781 aaacgcctga aattttattt tggattggtt gtgctggtag ctttgaccaa cgtgcacaaa
     3841 aaataactaa agcctttgct gctatattag ataaaacagg tattaaatac gcaattttag
     3901 gtaaagaaga agcatgtact ggcgaccctg caagaagagc tggtaatgaa tttctatttc
     3961 aaatgatggc ctatcaaaat atccaaattc taaatggata tggaataaaa aaaattgtaa
     4021 caacctgccc acattgttta aatataatga aaaatgaata ccccgaatta gggggtaatt
     4081 acgaagtaat acatcataca actttactac aacaactaat taatgatggt aaaattaaac
     4141 ttaaagaagg tggcagcttt aaaggcaaaa aaattactta tcacgatagt tgttatttag
     4201 gtagagccaa taatatttat gaagcaccca gaaaagtttt agaagcttta gatgctgaat
     4261 tagttgaaat gaaacgttgt ggcagcaaag gtttatgctg tggtgcaggt ggtgcacaaa
     4321 tgtttaaaga agaagaagca ggaaataaaa gaataaatat tgaacgtaca gaggaagcat
     4381 tagaaacaaa agcaaacttt attgcatctg cctgtcctta ttgcaataca atgatgaccg
     4441 atggtgtaaa actaaaagaa aaagaagcag atgtgcaagt attagatata gcagaaatga
     4501 ttgccaacga aatggaataa ttttataaaa aattaatgaa tgaaaaactt taacgtaaaa
     4561 aaatttataa aagcaacctt aatattacta cctattttgc ttttggttga tatagtgtac
     4621 gacatgattt ttaaagaatt aattcttaaa gaaacctttg caatgaaaaa tttatttttc
     4681 aaaattgcag cttctttagt tggtgcttat ttttttgcca cttctaaaac gaaagaggaa
     4741 aactaacttt aatttttatt tcataaaata attaattatt tgaatggata tttcttattc
     4801 acacctaatt ccaaccaatt ttagcgataa tgcaaaagtt tggatttacc aaagtagtag
     4861 aaaatttaca ataaacgaag ctttgcaaat tgaagctatt ttagacgact ttattgcaca
     4921 atggcaaagc catggcatac ctgtaaaagg ttttgctacc ctgttttttg gacagttttt
     4981 ggtttttatg gcagatgata atgttgatgt tagtggctgt agcactagct cctcggtgga
     5041 tgttgtaaaa caagttgaaa aaatatttaa tgtagaaatg tttaaccgac aaaatttggc
     5101 atttgttgtt aaagatgaag ttcagttatt gcctcttgca cagttaaact atgcattatc
     5161 ca
//
LOCUS       DAOQPK010000157         5151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_9936_length_5151_cov_5.239992, whole genome shotgun sequence.
ACCESSION   DAOQPK010000157 DAOQPK010000000
VERSION     DAOQPK010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9936_length_5151_cov_5.239992"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PLU36_07825"
     CDS             <1..229
                     /locus_tag="PLU36_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HMZ46695.1"
                     /translation="LADKKNHRLLLFAEMKLPGEAWLEFSIINNNNRSILKQTATFRP
                     KGIMGRNYWYSMLPFHYFIFRNMIKRIAGKK"
     gene            256..1431
                     /locus_tag="PLU36_07830"
     CDS             256..1431
                     /locus_tag="PLU36_07830"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HMZ46696.1"
                     /translation="MIAVSEAKKLIAENCIYHKIIPIKIGDALDYVLAENIYAPFNSP
                     HFIQSAMDGYAFNFDNWNGKDEFTIVGEVQAGKCYDGPINKAEAVRIFTGAALPFNAN
                     TVVIQEHVEKKGNAIKILDTNLKNGSNVRPIGSQTKQNELLLEKGHLLTPASIALLAN
                     FGIKTISVFSNPTISIITTGKELLQLNEELVEGKVYESNSFALAAVLKQMKITPTSIE
                     MVDDDEGLIINAIKNTKNADILILTGGVSVGDYDFVNAALEKCGAIKIFHKVKQKPGK
                     PFYFGKINHTLVFGLPGNPASVLSCFYMYVAEAVGYYTQKKYFKNATVTLAHHFQKKA
                     GLTFFLKGKTDNNDVIILDNQESYKMNSFALANCLIELNPEKEFFEKGELVHIKLIV"
     gene            1536..2129
                     /locus_tag="PLU36_07835"
     CDS             1536..2129
                     /locus_tag="PLU36_07835"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HMZ46697.1"
                     /translation="MKPLSIKIIIALYIIIASCNSATSSKSEEKLANKDSADLCLKPV
                     AASDTAKFISKQIIIKGDVEREVILTVDSLKKMQTKILNNLNVVCQTGITTSKHKTKT
                     GVLLKDIIDKAGIKKTNHKDRNFVIVARATDNYKATFSWAEIYNNPTGENTYVLFEED
                     GQPIKTHGDMKLICANDIKTGPRQVIWLKSIEINRIN"
     gene            2145..2885
                     /locus_tag="PLU36_07840"
     CDS             2145..2885
                     /locus_tag="PLU36_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HMZ46698.1"
                     /translation="MNFEVYSVLFFGLLFIVAFLYASVGHGGASGYLALMAFFSFAPE
                     VMRPTALLLNIFVSFTAFIQYYRGNHFKWKLFWPFAIASFPAAFLGGLIVIDVKLYKL
                     ILGVLLLFCVIRLIGIKFNNNNKPKEQSLILSLIIGAAIGLFSGMIGIGGGIILSPVI
                     LLLHWGNMKQTAAVSALFILVNSISGMAGLLTKGIDVSNNMIVMIAIAFAGGLLGSYV
                     GAIKATNSFLKTLLATVLLLASYKLLFT"
     gene            2889..3485
                     /locus_tag="PLU36_07845"
     CDS             2889..3485
                     /locus_tag="PLU36_07845"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HMZ46699.1"
                     /translation="MEKFKHIDAFVLAGGKSSRMGTDKGLLLFENKHLVEWAIDELQL
                     IFDKVIIVSNKQVYKKFKLEVIKDVKKNLGPAGGIYSILKHSKTNYIVVVSCDMPFIN
                     SNAIAFLINQCNDYDITIPVYKNKLQPLCAVYSKNCLVKWKELIDKNEIKLQNMIEHF
                     NVLKINVDDNSLFNESQFVNINDRNDFENAFQLLKYAN"
     gene            3475..3708
                     /locus_tag="PLU36_07850"
     CDS             3475..3708
                     /locus_tag="PLU36_07850"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HMZ46700.1"
                     /translation="MQINVLAFGQIAEAMNGESFTFSNINTTNDLIDYLKQQYASLKT
                     LDYKIAVNKQIIHSNMPLNNHDTVALLPPFSGG"
     gene            3710..4834
                     /locus_tag="PLU36_07855"
     CDS             3710..4834
                     /locus_tag="PLU36_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HMZ46701.1"
                     /translation="MNNHLYDRYQRQILLKEFGTEAQAKLFNAKVLVIGAGGLGCPAL
                     QYLAAAGVGTIGIVDFDLIELSNLQRQTLYNTNDIGKHKTTVSAERINAFNPSIHIQT
                     HTIQLNNTNAYNLIYNYDIVIDGTDNFSTKYLINDVCTILQKPLIYGAILRFEGQVSV
                     FNLPNKQNICTNYRDLFATPPLPNNVPTCNEAGVIGVLPGIIGTMQAAEAIKIITNIG
                     EPLCNKILSYNVLNNKSYLFDILPITHHSNNFPTTKTEIENFNYDWHCGLNDTVTEIE
                     FYEFEELRKNEEIKIIDVREEGEIPKVEAFSCINIPLSNFVNKIQNIELTNRVVVFCQ
                     HGKRSIKAAQILKAKYNHLNIVSLKGGIVEWEKQFNDSIY"
     gene            4846..>5151
                     /locus_tag="PLU36_07860"
     CDS             4846..>5151
                     /locus_tag="PLU36_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HMZ46702.1"
                     /translation="MEKEKIKNIFIEGAITAEFIGNSIYKHSTKKNIGAHSIFLGQVR
                     NDIIKEQQVKAIEYTAYTEMANEKFYEIREDAFKKYPLVCMHIYHSLGIVNAGEISLF
                     "
BASE COUNT         1932 a          656 c          860 g         1703 t
ORIGIN      
        1 tttagccgat aaaaaaaacc atcgccttct tttatttgcc gaaatgaaat tacccggtga
       61 ggcatggtta gaattttcta tcataaataa taacaatcgg tctatcttaa aacaaacggc
      121 aacctttcgc ccaaagggta taatgggtcg taattactgg tattctatgt tgccatttca
      181 ttattttatt tttagaaata tgattaaacg aattgcgggc aaaaaataat ttgtataaat
      241 atttagaaag gaaaaatgat tgcagtatct gaagcaaaaa aattaattgc agaaaattgt
      301 atctaccata aaattatacc tattaaaatt ggagatgcac ttgactatgt tttagcagaa
      361 aacatttacg ctccttttaa ttctccacat ttcatacaat ctgcaatgga tggatatgca
      421 tttaattttg ataattggaa tggaaaagat gaatttacaa ttgtaggaga agtgcaagca
      481 ggtaaatgtt atgatggccc aattaataag gcagaagctg taagaatttt tactggtgct
      541 gcattgccat ttaatgccaa tactgttgta atacaagagc atgtagaaaa aaaagggaat
      601 gctataaaaa ttttggatac taatttaaaa aatggcagta atgtaaggcc aattggctct
      661 caaactaaac aaaatgaatt attgttagaa aaaggtcact tgcttacacc agcttctatt
      721 gccttgttgg caaactttgg aataaaaact atttcagtat tttcaaaccc cactataagc
      781 ataataacaa caggcaaaga acttttacaa cttaatgaag aattggttga aggaaaagtt
      841 tacgaatcaa attcgtttgc attagctgct gtattaaaac aaatgaaaat tacaccaaca
      901 tcaatagaaa tggttgatga tgatgaaggg ctaataatta atgctatcaa gaatacaaaa
      961 aatgctgata ttttaatcct tacaggaggc gtatctgttg gagattatga ttttgtaaat
     1021 gcagcgttag aaaaatgtgg agcaataaaa atatttcata aagtaaaaca aaagcccgga
     1081 aagccatttt attttggaaa aattaaccat acgctagtat ttggtttgcc aggcaatcca
     1141 gcatcggtat tgtcttgctt ttatatgtat gtggccgaag ctgttggcta ttatacacaa
     1201 aaaaaatatt tcaaaaatgc aacagtaaca ttagctcatc attttcaaaa aaaagcaggg
     1261 ctaacctttt ttttaaaagg aaaaacagat aacaacgatg ttattatttt agataatcag
     1321 gaaagctata aaatgaactc ttttgcacta gcaaattgtt taattgaact aaatccggag
     1381 aaagaatttt ttgaaaaagg agaattagtt catataaaac tgattgtata attctagtca
     1441 tattttttta cattctaact ttttattttg aggtcttaaa tcttgcagca taaagtattg
     1501 tgctaaaatg cacactatta ataattcagc aaaatatgaa acctttatct atcaaaataa
     1561 ttattgcact gtatataata atagcaagtt gcaatagtgc aacaagtagt aaatcagaag
     1621 aaaaattagc aaataaagat tcggcagatt tatgtttaaa gcctgttgca gcaagtgata
     1681 cagccaagtt tattagtaag caaattataa ttaaaggaga tgtggaaaga gaggtaatat
     1741 taactgttga ttccttaaaa aaaatgcaaa ccaaaatatt aaataatcta aatgttgtat
     1801 gccaaaccgg tattacaaca tctaaacata aaacaaaaac aggggtatta ttaaaagata
     1861 taattgataa ggcaggcatt aaaaaaacca atcataaaga tagaaatttt gttatagtag
     1921 caagggcaac agataattat aaagcaacat tttcgtgggc agaaatttat aataacccaa
     1981 caggagaaaa tacgtatgta ttatttgaag aagatggaca gccaataaaa acacatggcg
     2041 atatgaagct aatttgtgcc aatgatatta aaactggacc aagacaagtt atttggctaa
     2101 aatcaattga aataaataga ataaactaat tgctaattta tacaatgaat tttgaagtat
     2161 atagcgtttt gttttttggc ttactattta ttgttgcatt tttatatgca tcagtagggc
     2221 atggcggtgc tagtggttat ttagcattaa tggcattttt ttcatttgct ccagaagtaa
     2281 tgaggccaac tgctttgcta ctaaatattt ttgtttcgtt tacagctttt atacaatact
     2341 atagaggcaa tcattttaaa tggaaattat tttggccctt tgctattgca tcttttcctg
     2401 ctgctttttt aggaggccta attgttattg atgtaaaatt atataaatta attcttggtg
     2461 ttttgttgtt attctgtgtt attagattaa taggcataaa gtttaataat aataataaac
     2521 ctaaagaaca aagccttatt ttatcattaa ttattggtgc tgcaattggt ttattttctg
     2581 gtatgattgg tattggtggt ggtattattt taagtcctgt tattttatta cttcattggg
     2641 gcaatatgaa acaaacagca gcggtatcgg ccttatttat tttagttaat tctatttctg
     2701 gtatggcagg cttactaacc aaaggaattg atgttagcaa caatatgata gtaatgattg
     2761 caatagcatt tgctggcgga ctacttggct cttatgttgg tgctattaaa gcaacaaact
     2821 cgtttttaaa aactttatta gctacagtgt tattactggc ttcatataaa ttattgttta
     2881 cgtagtttat ggaaaaattt aaacatatag atgcatttgt gcttgcaggt ggcaaaagct
     2941 caagaatggg aacagacaag ggtttgctct tatttgaaaa taaacatttg gttgaatggg
     3001 caattgatga attgcaatta atttttgata aagtaattat agtttccaat aaacaagtgt
     3061 ataaaaaatt taagttagag gtaattaaag atgttaaaaa aaatcttggt cctgcgggtg
     3121 gtatttattc cattttaaaa cattctaaaa caaattatat agttgttgta agttgcgata
     3181 tgccctttat taattctaat gctatagcat ttttaattaa tcaatgcaat gactacgata
     3241 ttacaatacc tgtgtataaa aataaattac aacctttgtg tgcagtatat tctaaaaatt
     3301 gtttagtaaa atggaaagaa ttgattgata agaatgaaat taaattgcaa aacatgattg
     3361 aacattttaa tgtactaaaa attaatgtgg atgataattc tctttttaat gaatcgcaat
     3421 ttgtaaatat taacgataga aacgattttg aaaatgcatt tcaattatta aaatatgcaa
     3481 attaatgtat tagcctttgg acaaattgca gaagcaatga atggagaaag ttttactttc
     3541 agcaatataa ataccactaa tgatttgata gattatttaa agcaacaata tgcgtcctta
     3601 aaaacattag actataaaat tgctgtgaat aaacaaatta ttcattcaaa tatgccacta
     3661 aacaaccacg atactgtagc tttattacca cccttttcag gaggttaata tgaataatca
     3721 tttatacgat agataccaac gacaaatttt attaaaagaa tttggaacag aagcacaagc
     3781 taaacttttt aatgctaaag tattagtaat tggtgcagga ggcttgggtt gccctgcatt
     3841 gcaatatctt gctgctgctg gtgttggtac aattggtatt gttgattttg atttgataga
     3901 gctttccaac ttacaaaggc aaacattgta taatacaaat gatattggca aacataaaac
     3961 tactgtttca gcagaaagaa ttaatgcgtt taatccttct attcatatac aaacacatac
     4021 catacaatta aataatacta acgcctataa ccttatttat aattatgata ttgtaattga
     4081 tggtacagat aatttttcta ctaagtattt gataaatgat gtttgtacaa tactgcaaaa
     4141 acctttaata tatggtgcta ttctacgatt tgaagggcag gtaagtgttt ttaatttacc
     4201 taataaacaa aatatatgca ccaattatag agatttattt gctaccccac cattgcccaa
     4261 taatgttcct acgtgtaacg aggctggagt aattggtgtg ctgccaggaa taattggaac
     4321 aatgcaggct gccgaagcta ttaaaattat taccaatatt ggagagcctt tgtgcaataa
     4381 aattttatcg tataatgtac tcaacaataa aagttattta ttcgatattt taccaattac
     4441 acaccatagt aataactttc ctaccactaa aacagagata gaaaatttta attacgattg
     4501 gcattgtggg ttaaatgata ctgtaacaga aatagagttt tatgaatttg aagagttgag
     4561 aaaaaatgaa gaaataaaaa ttattgatgt tagagaagaa ggtgaaatac caaaagtaga
     4621 agcgtttagt tgtataaata ttccattaag taattttgta aataaaattc aaaatattga
     4681 acttacaaat agggttgttg tattttgcca gcatggcaaa agaagtatta aagcagcaca
     4741 aatattaaaa gcaaaatata atcacctaaa tattgttagt ttaaaaggcg gaatagtaga
     4801 atgggaaaaa cagtttaatg atagtattta ttaaatgaga taacaatgga aaaagaaaaa
     4861 ataaaaaata tttttataga gggtgccatt actgcggagt ttattggcaa ttctatttat
     4921 aaacatagca ctaaaaaaaa tattggtgca cacagtattt ttttaggtca ggttagaaat
     4981 gatataatta aggagcaaca ggtaaaagct atagagtaca ctgcatatac ggaaatggca
     5041 aatgaaaaat tttatgaaat acgtgaagat gcttttaaaa aatacccact ggtttgcatg
     5101 cacatttatc atagcctggg cattgtaaat gctggagaaa ttagtttatt t
//
LOCUS       DAOQPK010000158         5123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_10030_length_5123_cov_4.555051, whole genome shotgun sequence.
ACCESSION   DAOQPK010000158 DAOQPK010000000
VERSION     DAOQPK010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10030_length_5123_cov_4.555051"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(254..481)
                     /locus_tag="PLU36_07865"
     CDS             complement(254..481)
                     /locus_tag="PLU36_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46703.1"
                     /translation="MKRVLVTVILILFVWQLNAQCSICTKTASQLGERPAAALNNAIV
                     YLMMTPLLIIGFVGYKWWKREKEIMKEEQSA"
     gene            561..1790
                     /locus_tag="PLU36_07870"
     CDS             561..1790
                     /locus_tag="PLU36_07870"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMZ46704.1"
                     /translation="MKNNFPLSGFSSVALHAANNSNNAYAHSTPIYATSTFVFDNAQQ
                     GMERFESEDKEKIYSRWSNPTFKVAEETIAAFEAFNIKTNNGNTIQLKALLHASGQAA
                     MTTMFLSCLQVGDTVLSHYSLYGGTYELMYKVLRQAGINIEIVNFKNLNEVEDRIKQN
                     KTIKFIHIETPANPTIQCVDIEAISTLAKQFNIKVSVDNTFATPYLQQPFQFGVDFVL
                     HSTTKFLNGHGTSIGGVLLGSDIDFMKQKVWKWFALLGGNANAFDAFMLINGMKTLEL
                     RMERHCSNAKQVASFMQQHHAIAQVNYNGLESHVDYALTLKQMKHAGALLSFELKGGI
                     ESGKKFIDNLKMCTRAVSLGTVDTLVSHPASMTHYSVPKEERIKFGITDGLIRVSIGI
                     ENVEDILNDFDQALHSV"
     gene            complement(1803..2672)
                     /gene="era"
                     /locus_tag="PLU36_07875"
     CDS             complement(1803..2672)
                     /gene="era"
                     /locus_tag="PLU36_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HMZ46705.1"
                     /translation="MKSGFVNIFGKPNAGKSTLLNALMGEKLAIVSHKVQTTRHRIKG
                     FLTEPEKYQIIFSDTPGIIEAKYRLHEKMMLAVKNALEDADVALLIVDVNDSLDEADA
                     IFKQLNLKVPCILILNKIDEAKNGKIAEAEKFFSDKPYAKKIIKISALQQHHLSKLLN
                     AILELLPEGAPFYNEDDLTDLPTRFFVGELIREKIYTLFEDEIPYHVAVLVNEYKEKD
                     TLVKIQADIIVQRETQKSIIIGGGGKMIKQIGTIARKDIEQFIGSKVFLELFVKVKPK
                     WRDNELQLKEYGY"
     gene            complement(2744..2815)
                     /locus_tag="PLU36_07880"
     tRNA            complement(2744..2815)
                     /locus_tag="PLU36_07880"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2781..2783),aa:Met,seq:cat)
     gene            complement(2867..3598)
                     /locus_tag="PLU36_07885"
     CDS             complement(2867..3598)
                     /locus_tag="PLU36_07885"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HMZ46706.1"
                     /translation="MKKIVCLLLCIIGIINLNAQNTNTVVNDPNAELRDVDEFKSIEV
                     SSGFIVYISQGKTQAVAVSCSNEKNNEDIITTVENGVLKIYRPKSTFKLLHKNQKFRA
                     YITVKNIEKLDISGACIVNLVNPIKADDLEIDISGASIIKGEVNAKHLNSDVSGASIS
                     TLKGNVASARINVSGASILKAFNLLVEEYDINVSGASIANIYVTQKLIASASGTSSIK
                     YKSTTENIKVNSTGLSNISEYNKDN"
     regulatory      3677..3782
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3844..>5123
                     /locus_tag="PLU36_07890"
     CDS             3844..>5123
                     /locus_tag="PLU36_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ46707.1"
                     /translation="MKKFMGTLLFLFITAFAFAQKTKITGKVIDEQTNLPLYGATVKL
                     NSLITITDEQGYFIFKNVKKQPFTILVSNIGYLDYSSEIKNLESSNDLIIQLKRQVYF
                     LDALEVTSIRAADKSPFSKNNLYKEQLAKLNVGQDLPFVLNTLPSVVINSDAGNGIGY
                     TGIRIRGTDATRINVTVNGIPFNDAESQGSFFVNMPDLVSSTNTIQVQRGVGTTTNGT
                     GAFGATINLSTNEVIEKPYTEINNTVGSFSTWKSTIKAGTGLLNKHFTLDTRLSKILS
                     NGYVQRASSNLQAFYVSAAYIAKNTSLRLNIFSGNEKTYQAWNGIDEATLKSNRTYNS
                     SGTEKLGEPYKNETDNYKQTHYQLFFNHKANQKWQFNIATFLVRGLGYYENYKANQKF
                     SKYGLPDVIIGASTITKTDLVRQLWLDNYFYGTI"
BASE COUNT         1751 a          792 c          818 g         1762 t
ORIGIN      
        1 gctacaatag atccttcgct tcgctcagga tgacaagaaa aggggagcag gatgacagta
       61 agtatataat gtcattccga gcgaatagaa acaaaatagt attgtcattc cgagcttgcg
      121 aggaatctaa atgtactttg gtacaaaacc tcagtagatc cttcgctacg ctcaggatga
      181 caagaaaaag tgtgcagagg attgacctta aaaaggaaga ataatatgga aagatggtag
      241 caaaaaataa gaattaggca ctttgttctt ccttcataat ttctttttct cttttccacc
      301 atttgtaacc aacaaagcca ataattaata aaggcgtcat cattaaatat acaattgcat
      361 tatttaaggc agcggcaggt ctttctccta attgcgatgc tgttttagtg caaatagaac
      421 attgtgcatt aagctgccaa acaaaaagta ttaatattac agttacaagt actcttttca
      481 ttatatggca aagataaaac aatccattct aaaagcagtt ttgtactgca ttttgtaatt
      541 ttgattttat aaataattgt atgaaaaata attttccact ttcagggttt agctctgttg
      601 cattacacgc agcaaataat agtaacaatg cttatgctca ttccacacca atttatgcta
      661 cctcaacctt tgtgtttgat aatgcacaac aaggaatgga acgttttgaa agtgaagata
      721 aagagaaaat ttactcacgt tggagtaacc caacatttaa ggttgccgaa gaaacaattg
      781 ctgcattcga agcttttaac attaaaacaa ataatggtaa tactatacaa cttaaagcct
      841 tgttacatgc aagtggccaa gcagcaatga ccaccatgtt tttaagctgc ttacaagtag
      901 gcgatactgt tttgtctcat tactccttat atggtggcac ttacgagtta atgtataaag
      961 ttttaaggca agctggaata aatattgaaa ttgtaaattt taaaaacttg aatgaagtag
     1021 aagatagaat aaaacaaaat aaaactatta agtttattca tattgaaaca ccagctaatc
     1081 ctactattca atgtgtagat atagaagcta tttctacatt agcaaaacag tttaatatta
     1141 aagtatctgt tgataatact tttgcaacac cttatttaca acagcctttt caatttggtg
     1201 ttgattttgt tttacatagt actacaaagt ttttaaatgg gcatggtact tctattggtg
     1261 gtgttttgtt aggaagtgat attgatttta tgaagcaaaa agtatggaaa tggtttgctt
     1321 tattaggagg taatgcaaat gcttttgatg cttttatgct aattaatgga atgaaaactt
     1381 tagaattaag aatggagagg cattgtagca atgcaaagca agtagcctct tttatgcaac
     1441 agcaccacgc cattgcacaa gtgaattata atgggttaga aagccatgtt gattatgcac
     1501 ttacattaaa gcaaatgaaa catgctggtg ctttactgag ctttgaacta aagggtggta
     1561 tagaatcggg taaaaaattt attgacaatc ttaaaatgtg tacaagagca gtttccttag
     1621 gtactgtaga tactttagtt tcccatcctg caagtatgac acattatagt gttcctaaag
     1681 aagaaagaat aaaatttgga attactgatg gcttaataag ggttagtatt ggtatagaaa
     1741 atgtagaaga tattttaaac gattttgacc aagctttaca ttctgtttaa attttttaaa
     1801 acttaatacc catactcttt caattgtagt tcattatctc tccatttagg ttttactttt
     1861 acaaatagtt ctaaaaaaac tttactgcca ataaattgct caatatcttt tcttgcgatt
     1921 gttccaattt gttttatcat ttttccgccg ccgccaatta ttattgattt ttgcgtttct
     1981 ctttgcacaa taatatctgc ttgaattttt actaaggtat ctttttcttt atattcatta
     2041 actaaaacag caacatggta aggtatttca tcttcaaata aagtataaat tttttctctt
     2101 atcaattcgc caacaaaaaa tctagtaggt aaatcggtta aatcatcttc attataaaaa
     2161 ggggcacctt ctggcagcaa ttctaaaatt gcatttagta gtttagataa atggtgttgt
     2221 tgtaatgcag aaattttaat tatttttttt gcataaggct tatccgaaaa aaatttttca
     2281 gcttctgcta ttttaccatt tttagcttca tcaattttat ttaatattaa aatgcaaggt
     2341 actttaaggt ttagttgttt aaatatggca tctgcttcat ctaagctatc atttacatct
     2401 acaattaata aagctacatc tgcatcttct aaagcatttt ttactgcaag catcattttt
     2461 tcatgaagcc tgtatttagc ttcaataatg ccaggtgtat cagaaaaaat aatttgatac
     2521 ttttcaggtt ctgttaaaaa accttttatt ctatgcctgg ttgtttgaac cttgtgagat
     2581 acaattgcta atttctcccc cattaatgca ttcagtaaag tactttttcc tgcattgggt
     2641 ttaccaaaaa tatttacaaa accagatttc atattgtata aaaatagtat gctttaagga
     2701 aatggtataa aaaaataaag cccaacattg ggctttattt tagttgcgag gatagggttc
     2761 gaacctatga ccttcgggtt atgagcccga cgagctaccg ctgctctacc tcgcggtatt
     2821 atggagtgca aaagtagcgg tatgcaatat actaccaaaa tttattttaa ttgtccttat
     2881 tatactctga aatattggat aaacctgtac tgttaacttt tatattttct gttgtgcttt
     2941 tatatttgat gctacttgtg ccgcttgcag aggcaattag tttttgagta acataaatat
     3001 tagcaatgct tgccccactt acatttatat catactcttc aaccaataag ttaaatgctt
     3061 ttaaaatact tgctccacta acatttatac gagcactagc aacattacct ttaagtgtag
     3121 atatgcttgc accacttaca tcactattca agtgcttggc attaacttcg ccttttataa
     3181 tacttgcacc agaaatatct atctctaagt catcagcttt tattgggtta accaagttta
     3241 caatacaagc accagaaata tctaattttt ctatattttt tacggttata taagccctaa
     3301 atttttggtt tttgtgtagt agtttaaatg tagattttgg tctgtaaatt ttcaatacac
     3361 cattttcaac agtagtaata atatcttcat tattcttttc attactgcaa cttacagcaa
     3421 cagcttgtgt tttaccctga gaaatatata caataaaacc actagatacc tcaatgcttt
     3481 taaattcatc tacatctctt aattctgcat tagggtcgtt tacaactgta ttagtatttt
     3541 gtgcatttaa attgattata ccaataatac aaagcagtaa gcatactatc tttttcattg
     3601 tgctattttt atatttaacg aataaagaga ataaaggtta cattatatca ttaatttatc
     3661 ttgtacattt gcaccaaaca attcctcggg gtgcttattc tttttttaaa agaatggctg
     3721 agattacacc cgtagaacct gaacagggta attcctgcga agggaaggcg gcttctatat
     3781 ttatagtcaa ctttctagag cgttattcct gttcccattt ttaaaattat aaaatttttt
     3841 acaatgaaaa aattcatggg aacattgctg tttttattta ttacagcatt tgcatttgca
     3901 caaaaaacaa aaattaccgg caaagttatt gatgaacaaa caaatttgcc attgtatggt
     3961 gccacagtta aacttaacag tttaattact attacagatg aacaaggtta ctttattttc
     4021 aagaatgtaa agaaacagcc atttactatt ctagtaagca atattggtta tttagattac
     4081 tcaagtgaga taaaaaattt agagagtagt aatgatttaa taatacaatt aaaaaggcaa
     4141 gtgtattttt tagatgcttt agaagtaaca tctattagag ctgcagataa atctcctttt
     4201 agcaaaaaca atttatataa ggagcaattg gcaaagttga atgttggaca agatttacct
     4261 tttgtattaa acaccctacc atctgtagta attaattcag atgcaggcaa tggcataggt
     4321 tatactggta ttagaattag aggtacagat gccaccagaa taaacgtaac tgtaaacgga
     4381 attcctttta atgatgcaga aagccaggga tcattttttg taaatatgcc agatttggtt
     4441 tcttctacaa acacaattca ggtacaaaga ggagttggta caacaaccaa tggtacagga
     4501 gcatttggtg ctacaataaa cttaagtaca aatgaagtaa tagaaaaacc ttataccgaa
     4561 attaataata cagttggctc ttttagcacc tggaagagta caataaaagc aggtactgga
     4621 ttattgaata agcattttac tttagatacc aggcttagta aaattttgag taacggctat
     4681 gtacaaagag caagtagtaa cttacaagcc ttttatgtat cagcagcata tattgcaaaa
     4741 aatacatctt taaggctaaa tattttttct ggtaatgaaa aaacttatca ggcatggaat
     4801 ggtatagatg aagctacttt aaaaagcaac agaacatata actcaagtgg taccgagaaa
     4861 ttaggagagc cgtataaaaa cgaaacggat aattacaagc aaactcatta tcaacttttt
     4921 ttcaatcata aagcaaatca aaaatggcag tttaatattg ctactttttt agtgagaggg
     4981 ttgggttatt atgaaaacta taaagcaaat caaaagttta gcaagtatgg tttacctgat
     5041 gtaataattg gtgcatctac aattaccaaa acagacttag taaggcaatt atggcttgat
     5101 aattattttt atggaactat ttt
//
LOCUS       DAOQPK010000159         5096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_10102_length_5096_cov_4.166435, whole genome shotgun sequence.
ACCESSION   DAOQPK010000159 DAOQPK010000000
VERSION     DAOQPK010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5096
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10102_length_5096_cov_4.166435"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..1607
                     /gene="rlmD"
                     /locus_tag="PLU36_07895"
     CDS             186..1607
                     /gene="rlmD"
                     /locus_tag="PLU36_07895"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HMZ46708.1"
                     /translation="MKKRRQPTILQNILVEDYAAEGKSLAKIDGKVIFIERAVPGDTV
                     DIQLFKNKKDWAEGFPINFISLSKDRVEPFCAHFQICGGCQWQMLPYQKQLAFKQKQV
                     SDNLKRIGKIDLPEILPIVGAEQTQFYRNKIEYTFANKRYVPDEELAYLEHNNLPFNN
                     EGSFGGFHAKGVFDKVVEIKKCYLQAEPTNEIRNAITAFCIQNNYPFYDIKNHTGWLR
                     NIMVRIATTGEWMVNIMLGFEKEKWRTDLLDFVLQKFPTITTLLYTINTKFNDSIYDL
                     TPVAYYGKGFIEEKLEQYKYNISPKSFFQTNTKQAEKLYNITRDFAELTGSETVYDLY
                     CGTGSIGIFVSNKAKKIIGVETVSDAVEVAKINASLNNITHAHFFAGDVIKVCDDIFF
                     ATHGKADVIITDPPRAGMHEKLITKLLEIAAPIIVYVSCNPATQARDLALLNAKYIVT
                     KVQPVDMFPHTHHIENVVQLKLR"
     gene            1674..2372
                     /locus_tag="PLU36_07900"
     CDS             1674..2372
                     /locus_tag="PLU36_07900"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HMZ46709.1"
                     /translation="MREFRPTRFEILPVVVKNLLIINGLVFFAQITIGEKFNVNDIFA
                     LHTWQSPLFKPWQFITHMFMHGNFGHLFSNMFALWMFGSILENVWGPKRFLTFYLICG
                     LGAALCHMIVLYYDMQPIVEAFKTLNFEEQQEILFSNRFRLNEATLGASGAVFGCLAA
                     FGYLFPNTQIYIYFLFPLKAKWFVLIYAGLELMQAVQNSAGDNVAHVAHLGGALVGFL
                     LVYYWNKTNKQSLY"
     gene            2400..3320
                     /locus_tag="PLU36_07905"
     CDS             2400..3320
                     /locus_tag="PLU36_07905"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HMZ46710.1"
                     /translation="MNTKSINKNKKPLLGANDNGLVLLVAINALIFIVLNFLRIAYTV
                     SADTVAIGDANFQKQILHWFVLHSNANLLAEKPWTILLYMFTNFSIWSLLSNLLWLWA
                     FGYILQDLAGNKKMIPVYLYGGFIGGLVFLLSLSFIPSLYQYVSSGYFLIGSGTSVMA
                     VAVATTTLAPNYKIFPLINGGIPLWILMVIYAALQFGSLAMGNTGITIAYLFSASIGF
                     LYIQQLNKGKDWGSWMFTLVNWINDIFNPEKKYPNLKKNSSREEVLNNKTTIAQEKID
                     IILDKINKHGYDKLSKEEKEFLNNASNSEV"
     gene            3327..4463
                     /locus_tag="PLU36_07910"
     CDS             3327..4463
                     /locus_tag="PLU36_07910"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HMZ46711.1"
                     /translation="MALSLKKIFSNTILLTGCIYSFVYLLSCLTPFISPTVWSGFTFL
                     ALAFPILFCVMLFWLFIVFIFYKKYRWLFLIVTLLGSTNIFNIVAFNFPSKFMQEKQE
                     GNIRILSWNVQDFIDSQIYNDTPGSVRRKMMEFIKNTDADILCFQDFQEHTSTLFRSC
                     VKDITTQLNYPFKYFSRDFETKRYYAFIQYGTIIFSRYPIIDSGRTTYSNKNFSESLA
                     FADIKKGNDTFRIFNTHLKSMYLGLVMDTLAFKDEFIKDDLDFLMKNQDKFSRLKYYD
                     KVHAAQAKIVKEQLNKSKYPFIFCGDLNSVPSSYTYYTIQKNLTDAFKSKGFGFGRTY
                     REISPTLRIDAVLLSKQFKVLQYYSPQLSNASDHYPILVDVKLP"
     gene            complement(4688..>5096)
                     /locus_tag="PLU36_07915"
     CDS             complement(4688..>5096)
                     /locus_tag="PLU36_07915"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HMZ46712.1"
                     /translation="KILVLCTGNSCRSQIAEGYLQHFANSKAEIYSAGVETHGVNPRA
                     VATMKEDGIDISSHTSNNIDEYRSIDFDFIITVCDNAKERCPFFPSKAKKFHYNFPDP
                     AKATGTELEILQQFRSVRQLIKDYCKQFVADYL"
BASE COUNT         1759 a          710 c          822 g         1805 t
ORIGIN      
        1 aatacaaggg ctaaaagaat ttttttcatt ctaatcaatt ttaatgttca aaaaataaaa
       61 tattaacttt tgcaagtata actcaaaatt aaagttatta aacttacatt tttatttgtt
      121 tcagaaataa tcattcttta aatgttacat ttctgtatat caatattttc aatgaaattt
      181 gccttgtgaa aaaaagaagg caacccacca ttttacaaaa tattttggta gaagattatg
      241 cagccgaagg aaaatcgctt gcaaaaatag atggtaaagt aatttttatt gaaagggcag
      301 tacctggtga tactgtagat attcaactat ttaaaaataa aaaagattgg gccgagggct
      361 ttccaataaa ttttatttcc ctttctaaag atagagtaga accattttgt gctcattttc
      421 aaatttgtgg tggatgccaa tggcaaatgc ttccttacca aaaacaattg gcttttaaac
      481 aaaaacaagt aagcgataat ttaaaacgta ttggcaaaat agatttacca gaaattttac
      541 ctatagttgg tgcagagcaa acacagtttt acagaaacaa aattgaatac acatttgcta
      601 ataaacggta tgtgccagat gaagaattgg cttatttaga acataataat ttgcccttta
      661 acaacgaggg tagttttggt ggctttcatg caaaaggtgt ttttgataaa gtagttgaaa
      721 taaaaaaatg ctacctgcaa gcagagccta caaatgaaat tagaaatgca attactgcct
      781 tttgtattca aaataattat cctttttatg atataaaaaa tcatacaggc tggctaagaa
      841 atataatggt tagaattgcc accactggtg aatggatggt gaatataatg cttggttttg
      901 agaaagaaaa gtggagaaca gatttgttag attttgtact tcaaaaattt ccaaccatta
      961 ctacattact ttatactatt aatacaaagt ttaatgatag tatatacgat ttaacgccag
     1021 tagcctatta tggcaagggt tttatagaag aaaagctaga acaatataaa tataatatta
     1081 gtccaaaatc tttttttcaa accaatacaa aacaagcaga aaaattatac aatataacaa
     1141 gagattttgc tgaactaaca ggtagtgaaa cagtgtatga tttatattgt ggtacaggta
     1201 gtataggaat ttttgtaagc aataaagcta aaaaaattat tggtgttgaa actgtatcag
     1261 atgctgtaga agttgcaaaa attaatgcct ctttaaacaa tataacccat gctcattttt
     1321 ttgcaggaga tgttattaaa gtttgcgatg atattttttt tgcaacccac ggcaaggcag
     1381 atgtaataat tactgaccca ccacgtgcag gtatgcatga aaaattaatt actaaattat
     1441 tagaaattgc tgcaccaatt attgtatatg taagttgtaa ccctgctaca caagcaagag
     1501 atttagcctt acttaatgcc aaatacattg ttacaaaagt gcagcctgtt gatatgtttc
     1561 cacacacaca ccatatagaa aacgttgttc aactaaaatt aagataaatt tttacaaaag
     1621 taatatggtt aaatttttaa aatatacttt tggtaattca atatataaaa caaatgagag
     1681 aatttagacc aactagattt gaaattttac ctgttgttgt aaaaaacctg ctaattataa
     1741 acgggctagt tttttttgct caaataacta ttggcgaaaa atttaatgta aatgatattt
     1801 ttgctttgca tacctggcaa agcccattgt ttaaaccttg gcaatttatt acgcatatgt
     1861 ttatgcatgg caattttggg catttattca gtaatatgtt tgccttgtgg atgtttggct
     1921 caatattaga aaatgtttgg gggccaaaaa gatttcttac gttttattta atttgtggcc
     1981 taggtgctgc attatgccac atgattgttt tgtattacga tatgcaacca attgtagagg
     2041 catttaaaac attaaatttt gaagagcaac aagaaatatt attcagcaac agatttagat
     2101 taaacgaggc tacacttggt gcaagtggtg ctgtatttgg ttgtttagct gcgtttgggt
     2161 atttattccc taatacacaa atttatattt attttttatt ccctttaaaa gcaaagtggt
     2221 ttgtactaat ttatgctggg ctagagttaa tgcaagctgt tcaaaactct gctggagata
     2281 atgtagcaca tgtggcacat ttaggtggtg cattagttgg ctttttatta gtttattact
     2341 ggaacaaaac caacaagcaa tctctttact aactatttga agtatttttt aagccaacta
     2401 tgaatacaaa atctattaat aaaaataaaa aacctttatt aggagctaat gataatgggc
     2461 tagttttatt agtagcaata aatgcattaa tttttattgt tttaaatttt ttaagaattg
     2521 cctatacagt ttctgcagat acggttgcta ttggtgatgc taattttcaa aaacaaattt
     2581 tacattggtt tgtcttacac tccaacgcaa atttgttagc cgaaaaacca tggacaattt
     2641 tactttacat gtttacaaac tttagcattt ggtcgttgct ttccaattta ttatggcttt
     2701 gggcatttgg ttatatttta caagatttgg ctggcaataa aaaaatgatt cctgtttatt
     2761 tgtatggtgg ctttattggt ggcttagttt ttttactttc tctaagtttt ataccttctt
     2821 tatatcaata tgtaagttct ggctattttt taattggtag cggaacaagt gtaatggctg
     2881 tagctgttgc taccactaca cttgcaccta attacaaaat ttttccacta ataaatggtg
     2941 gtattccttt atggattttg atggttattt atgctgcatt gcagtttggt agtttagcta
     3001 tgggcaatac tggtataacc attgcttatt tatttagtgc ctctattggt tttttatata
     3061 ttcaacagtt aaataaaggt aaagattggg gcagttggat gtttacattg gttaactgga
     3121 ttaatgatat atttaatcct gaaaaaaaat atcctaacct aaaaaaaaat agtagcagag
     3181 aagaagtttt aaataataaa acaaccatag cacaagaaaa aattgatatt attttagata
     3241 aaataaataa acacggctac gataaactta gcaaagaaga aaaagagttt ttaaataatg
     3301 caagtaatag tgaagtgtaa tttaaaatgg cattaagttt aaaaaaaata tttagcaata
     3361 ccattctctt aacaggctgt atttatagtt ttgtttactt actaagttgc cttactcctt
     3421 ttataagtcc aacagtttgg agtggcttta cttttttagc attagcattt ccaatacttt
     3481 tttgtgtaat gctattttgg ctattcatcg tatttatatt ttataaaaaa tacagatggc
     3541 tgtttttaat tgttacactt ttaggtagta ctaatatctt taatatagtt gcatttaatt
     3601 ttccttcaaa atttatgcaa gagaaacaag agggaaacat tagaatttta agttggaatg
     3661 tacaagattt tatagatagc caaatttata acgatacgcc aggtagtgta agaagaaaga
     3721 tgatggagtt tattaaaaat accgatgcag atattttatg ttttcaagat tttcaagaac
     3781 atacttctac cctttttaga tcttgtgtaa aagatataac cacccaatta aactacccat
     3841 ttaagtattt tagtagagat tttgaaacaa aacgatatta tgcttttata caatatggta
     3901 ccattatttt ttcaagatat ccaattatag attctggaag aacaacttac tccaataaaa
     3961 atttttctga gtctcttgct tttgcagata taaaaaaagg taacgataca tttagaattt
     4021 ttaataccca tttaaagtct atgtatttag ggcttgttat ggatacctta gcatttaaag
     4081 atgaatttat aaaagatgat ttagattttt taatgaagaa tcaggataag tttagcagat
     4141 taaaatatta cgataaagta catgcagccc aagccaaaat agtaaaagag caattaaaca
     4201 aaagtaaata tccgttcatt ttttgtggcg atttaaatag tgttccatca agctatacct
     4261 attataccat tcaaaaaaat ttaaccgatg cttttaaaag taaagggttt ggctttggta
     4321 gaacttatag agaaatatct ccaacattaa gaattgatgc tgttttgttg agtaagcaat
     4381 ttaaggtttt gcaatattat tcgccccaat taagcaatgc ttcagatcat tatcctatac
     4441 tagttgatgt aaagcttcca taaaaaaaca gtatctaaca cattcagagt ttgaaggtgt
     4501 ttggtgccac tgtgtttctt catgcttaaa ccgaaaccaa atattgttga tgctgttaat
     4561 tgataataca aaagttaggt aagttttata gagcctatgg taataagaaa aaagaattac
     4621 aaaagctgat tcataattca tctgcccaaa tcctgcatac ccttcttcgt ggttgcttaa
     4681 agttgcttta taaatagtct gccacaaatt gtttgcaata gtcttttata agttgcctta
     4741 cactacgaaa ctgttgcaaa atttccagct ctgtccctgt tgcttttgca gggtccggaa
     4801 aattataatg aaactttttt gctttgcttg gaaaaaacgg acaacgttct tttgcattat
     4861 cgcaaacagt gattataaaa tcaaaatcaa tgcttctata ttcgtcaata ttgtttgaag
     4921 tatggcttga aatgtcaatg ccgtcttctt tcattgttgc aacagctctt ggatttaccc
     4981 catgagtttc aacgcctgca ctataaattt ctgctttgct atttgcaaag tgttgcaaat
     5041 aaccttctgc tatttggctt ctgcagctat tacctgtaca tagtactaat attttt
//
LOCUS       DAOQPK010000160         5094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_10108_length_5094_cov_3.894225, whole genome shotgun sequence.
ACCESSION   DAOQPK010000160 DAOQPK010000000
VERSION     DAOQPK010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10108_length_5094_cov_3.894225"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /locus_tag="PLU36_07920"
     CDS             <1..1476
                     /locus_tag="PLU36_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46713.1"
                     /translation="GVLQITIFNVNWLPIAERIFFVKTDNYESLVDARIFKPNTTKKA
                     KNNLEISIPDEMLTNISVAITDAGLTESEDENIISSLLLTSDIKGKVYNPLFYFSNNA
                     DSTKQFLDLVMQTHGWRKLKWMDIAQNKTPEITYPKDSAYVSVAGKVFGANASQLRQA
                     QSLNVFVKTKDSATKFLSLPLQPDGSFVQKDFIFFDTVTVMYQFNKYKDLSYSTAINF
                     SNLLLNSENKINIDSLYSYNDKEDTIGNAKKIQLWEQQKKLAKLSQSTTLDDVVVTTK
                     TKKPEDKLDAQYATGFFQGGDAYQFDMESNRNSAAISDVLTYLTGRIAGLTITHSGNP
                     PKITWRGGTPGLYLNEMLVDAATLQEIYVSDVAYIKVIKPPFFGGMGGSGSAGAIAVY
                     TKKGKNSNENNFKGLDKKRIAGYTAIREFYSPDYDMNPELRQEKDIRTTIYWNPYVIL
                     TSQNRTVLLNFFNNDVTKKFRLSIQGMNEEGKLISIHKIIE"
     gene            1616..2308
                     /locus_tag="PLU36_07925"
     CDS             1616..2308
                     /locus_tag="PLU36_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HMZ46714.1"
                     /translation="MFYLLQVAGNVADSVNKMNTAINTADKISMIDLLNKGGWIMYPL
                     YFLFAATIFVFTERFLAIRKAGKIDDNFMRIIRDNILSGNVTAARSMAKNTGNPVAKM
                     IDKGLQRIGKPIDAIEKSMENVGKLEIYKLERNTSILSLIAGIAPMFGFLGTIVGMIE
                     LFYNINNSGFELNTIAGGIYVKMVTSASGLIIGLIAYLAYNFLSTQIDKTVYKMEAAS
                     SEFIDVLQEPTK"
     gene            2338..2739
                     /locus_tag="PLU36_07930"
     CDS             2338..2739
                     /locus_tag="PLU36_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HMZ46715.1"
                     /translation="MNIRKRLRQKPDLHTSALNDILFILLLFFLIVSTLANPNVVKVN
                     NPKGKSDTKAKQNIVISIDKENKIYLGQAVIPENNLDSVIKVEVDKVKAFIDTPSVVI
                     NADTISYTGGFFKVMGAAKKAGAKIVANVKD"
     gene            2827..3963
                     /locus_tag="PLU36_07935"
     CDS             2827..3963
                     /locus_tag="PLU36_07935"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HMZ46716.1"
                     /translation="MTKILVVGAGMVGSAMALDLSKKYAVTVADINSTNLNKIQQKNN
                     AIQIISLDVTDETALTNCIVNFNFVVCAVPGFLGFKTLRTIINTGKSVVDISFFPENA
                     LELDALAKQKNVTAIVDCGVAPGMGNIIVGYQNTQMQINNFECLVGGLPKIKKWPFNY
                     KAPFSPIDVIEEYTRPARYIENGNLVVKPAMSDIEHIEMDGVGTLEAFNSDGLRSLIY
                     TMPHITNMKEKTLRYPGHIEKIIALRDAGFFNEEPIIINNTKITPLEFTSKILFKEWL
                     LGEEEEEITVMKIIISGTQNDQPIKVEYNLYDEYNATTKTSSMARTTGYTATAAVCLI
                     VENLFVEKGIFPPELVGKNAVCYNYIINYLLERGVKYKQEVTIG"
     gene            complement(3977..>5094)
                     /locus_tag="PLU36_07940"
     CDS             complement(3977..>5094)
                     /locus_tag="PLU36_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46717.1"
                     /translation="GEEEIYTSSYLGVYKRIFLKMKHPVADTLLKWVDYDYYKKFIKI
                     AANYNTLDDFLTRMNKANAEKLMRNFVTGLEKTKTLEDAVDVANSYASIYNNNIKKLI
                     LNQIEQNLLQSEKEQNKRGQIIYSLLNTIFLSMDSSQKIDLASRLGINGIYKMPISSL
                     KDSTGRIIIQQFFYGDKDGKSVFNSFFKYYHAPLWKKVEKENWVELYSTKGVPITIYC
                     NKPLDNELDLDLKAQEALGRYLEENELYPTVVIHRGHSYFVGSTIDQLPSSAKVVLLG
                     SCGGYQRLSDVLETCPESHIISSKQTGTGTVNIGLINAITHDLRLNKDLNWPLIWKNL
                     AKQFVGVSKERFDDYVPPHKNLGAIFIIAYNLALQVE"
BASE COUNT         1861 a          688 c          803 g         1742 t
ORIGIN      
        1 ggggttttac aaataactat atttaatgta aattggttac ctatagccga aagaattttt
       61 tttgttaaaa cagataatta tgaatcttta gtagatgcta gaatttttaa accaaacact
      121 acaaaaaagg caaaaaataa tttagaaatt agcataccag atgaaatgct taccaatata
      181 tctgtagcca ttacagatgc aggattaaca gaaagtgaag atgaaaatat tatctcctct
      241 ttactactta caagcgatat taaaggtaaa gtttataacc cattatttta tttttcaaac
      301 aatgcagata gcactaaaca gtttttagat ttagtaatgc aaacacatgg ttggagaaaa
      361 ttaaaatgga tggatatagc tcaaaataaa acaccagaaa ttacttaccc taaagactct
      421 gcttatgtat cggttgctgg taaagtgttt ggtgcaaatg catctcaatt aaggcaagca
      481 caaagcttaa atgtatttgt aaaaacaaaa gattcggcta ctaaattttt atcccttcct
      541 ttacaaccag atggcagctt tgttcaaaaa gattttatat tttttgatac cgtaactgtc
      601 atgtatcaat ttaataaata caaagattta tcgtattcta ctgcaattaa cttttctaat
      661 ttattactaa attcagaaaa taaaattaat atagatagct tgtatagtta taatgataaa
      721 gaagacacaa ttggtaatgc aaaaaaaata caattatggg agcagcagaa aaaattagca
      781 aaactaagcc agtcaacaac tttagatgat gtagtggtta ctacaaaaac taagaagcct
      841 gaagataaat tagatgcaca atatgctaca ggcttttttc aaggcggtga tgcatatcaa
      901 tttgatatgg aaagtaacag aaatagtgct gcaatatcag atgtattaac ttatttaaca
      961 ggaagaattg cagggcttac tataactcat tctggtaatc caccaaaaat tacatggcgt
     1021 ggtggcacac ctggtttgta tttaaatgag atgttggtag atgctgctac tttgcaagaa
     1081 atttatgtta gcgatgtggc ttatattaaa gtaattaaac ctccattctt tggtggtatg
     1141 ggtggttctg gctctgcagg agcaattgca gtttatacca aaaaaggtaa aaatagtaat
     1201 gaaaataatt ttaaagggct ggataaaaaa aggattgcag gatatacagc tataagagaa
     1261 ttttattcgc cagattatga tatgaaccca gaactgagac aagaaaaaga catacgaaca
     1321 actatttatt ggaacccata cgtaattctt acatctcaaa atagaacagt gttactgaac
     1381 ttctttaata atgatgttac taaaaaattc agattaagta ttcaaggaat gaatgaagaa
     1441 ggcaaattaa tcagtattca taaaattatt gagtaattat tcaatagata atatgcacat
     1501 gaaggagtaa atgtgcaaaa gctatcttaa aatataacaa acttctgttt ttattcacaa
     1561 ggcttttgtt tttttgtaat gctgagtatt ttagcctacc tcaaaattta tacaaatgtt
     1621 ttatttattg caagtagccg ggaatgtagc agattctgta aacaaaatga atactgctat
     1681 taatactgca gataaaattt caatgataga tttattaaat aaaggaggtt ggattatgta
     1741 tcctttatac tttttatttg ctgcaaccat ttttgttttt accgaacgtt ttttagctat
     1801 tcgtaaagct ggtaaaatag atgataactt tatgagaatt attagagata atatattatc
     1861 tggtaatgtt actgctgcaa gaagtatggc aaaaaatact ggtaatcctg ttgcaaaaat
     1921 gattgacaaa ggattgcaac gtataggtaa accaattgat gccattgaaa aaagtatgga
     1981 aaatgttggc aaactagaaa tttataaatt agaacgaaat acatctattc tatctttaat
     2041 agcaggtatt gcacctatgt ttggtttttt aggaaccatt gtaggtatga ttgaattgtt
     2101 ttataatatt aataatagtg gctttgaatt aaatacaatt gcaggtggta tttatgtaaa
     2161 aatggtaact agtgcctctg gtttaattat tggtttaata gcctatttag cctataattt
     2221 tttaagtaca caaatagata aaacagttta taaaatggag gctgcaagct ctgaatttat
     2281 tgatgtttta caagagccta ctaaataatt ttaatagaaa aataaagcat taaaattatg
     2341 aatatcagaa aacgattaag acagaaacca gatttacaca ctagtgcttt aaacgatatt
     2401 ttgtttattc tattactatt ttttttaatt gtatcaacat tggcaaaccc taatgtggtt
     2461 aaagtaaaca atcctaaagg caaaagcgat acaaaggcta aacaaaatat tgtaattagt
     2521 attgataagg aaaataaaat ttatttaggc caggcagtta tacctgaaaa taatttagac
     2581 tcagtaataa aagttgaagt agataaagta aaagcattta ttgatactcc ttctgttgta
     2641 attaatgctg atacaatttc ttatacaggt ggttttttta aagtaatggg agcagctaaa
     2701 aaggcaggtg ctaaaattgt tgctaacgta aaagattaat tttagttatt tactccaatt
     2761 tctttttcta taaaccttca agctattaat aattttgcta acttgaacaa gttacaaaat
     2821 ataattatga cgaaaatttt agttgttggt gcaggtatgg ttggcagtgc aatggcttta
     2881 gatttatcta aaaaatatgc tgtaaccgtt gcagatatta actcaaccaa tcttaataaa
     2941 attcagcaaa aaaataatgc tattcaaata atttcacttg atgttacaga tgaaactgca
     3001 cttacaaact gtattgtaaa ttttaatttt gttgtttgtg ctgttccagg ttttcttgga
     3061 tttaaaactt tgagaactat aataaataca ggaaaaagcg ttgtggatat ttcttttttt
     3121 ccagaaaatg cattagagtt agatgcttta gcaaaacaaa aaaatgttac tgcaattgtt
     3181 gattgtggcg tagcaccagg aatgggaaat ataattgtag gctaccaaaa tactcaaatg
     3241 caaataaata attttgaatg tttagtaggc ggtttaccca aaataaaaaa atggccattt
     3301 aattacaaag ctccattttc gccaatagat gttatagaag aatatacacg acctgcaagg
     3361 tatattgaaa atggaaattt agttgtaaag cctgccatgt cggatataga gcatattgaa
     3421 atggatggtg ttggaactct tgaagcattt aattctgatg ggttaagaag tttaatttat
     3481 accatgccac atataaccaa tatgaaagaa aaaactttgc gttatcctgg ccatattgaa
     3541 aaaataattg ctttaagaga tgcaggtttt tttaatgaag aacccataat tataaacaac
     3601 accaaaataa cacctcttga gtttacctct aaaatattat ttaaagaatg gttgttagga
     3661 gaggaggaag aagaaataac agtaatgaaa attattattt ctggaacaca aaatgaccaa
     3721 ccaataaagg tagaatataa tttatatgat gaatataatg ctacaaccaa aacatcgtca
     3781 atggcaagaa caacaggcta tacagctaca gcagcagtat gtttaatagt agaaaatttg
     3841 tttgtagaaa aaggcatttt tccaccagag ttggtgggta aaaatgcagt ttgttacaat
     3901 tatattatta actatttatt agaaagaggt gttaagtata aacaagaagt aactatagga
     3961 taaacatttt taaattttat tcaacttgta aagccaaatt ataagcaatg ataaaaatag
     4021 cacctaaatt tttatgtggt ggtacataat catcaaacct ttctttacta acacctacaa
     4081 attgttttgc taaatttttc caaattaagg gccaatttaa atctttatta agccttaaat
     4141 catgtgtaat ggcattaatt aaacctatgt ttactgtacc agtaccagtt tgtttagatg
     4201 aaatgatatg agattcaggg caagtttcta atacatcact taatctttga taaccaccac
     4261 aacttcctaa taaaactact tttgccgagc ttgggagttg atcaattgtt gacccaacaa
     4321 aataactatg gcccctgtgt attacaactg ttgggtacag ttcattttct tctaaatatc
     4381 ttcctaaagc ttcttgtgct tttaaatcta aatctagttc attatctaaa ggcttattgc
     4441 aataaatagt tattggaact ccttttgtgc tatatagttc tacccagttt tctttttcta
     4501 cttttttcca taagggtgca tggtaatatt taaagaaaga attaaaaaca ctttttccat
     4561 ctttatcgcc ataaaaaaat tgttgaataa ttattctgcc agtgctgtct tttaaactac
     4621 taattggcat tttgtaaatt ccattaattc ctaatctact ggctaaatct attttttgag
     4681 atgaatccat agataagaaa atggtattta gtaaactgta tataatttgc ccacgtttgt
     4741 tttgttcttt ttcgctttgt aaaaggtttt gttctatttg atttaaaatt aatttcttga
     4801 tattattatt ataaatgctt gcataagagt tagcaacatc tacagcatct tcaagtgttt
     4861 tagttttttc taaacctgtt acaaagtttc tcattaactt ttctgcattg gctttattca
     4921 ttcttgttaa aaaatcgtct agtgtattat agttagcggc aatttttata aactttttat
     4981 aataatcata atccacccat tttaataaag tatctgccac cgggtgtttc atttttaaga
     5041 aaattctttt ataaacaccc aggtaactgc tagtataaat ttcttcttcg ccta
//
LOCUS       DAOQPK010000161         5051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_10261_length_5051_cov_5.874700, whole genome shotgun sequence.
ACCESSION   DAOQPK010000161 DAOQPK010000000
VERSION     DAOQPK010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10261_length_5051_cov_5.874700"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1637
                     /locus_tag="PLU36_07945"
     CDS             <1..1637
                     /locus_tag="PLU36_07945"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HMZ46718.1"
                     /translation="KSTATTPLVSNNNIFYLGSAGSGAQSIGYQTSAQASLTAWQTAS
                     SQDLNSMVADPLFTNPSVGDYKPTETSIDNKGASLGVLRDILDTIRPLILPDLGAYEF
                     GTMPSPCSATPVPGNTVSSVPIACPNSNFTLSLEFPPSELGLTFQWQSADDAAFTTNV
                     VDLGTASTQATSQTSAKYYRCKVTCTNSGLFAYSTPIFIDINSWINCYCTPATTGGTT
                     YYISGFTTTGGSTNINKTTSAGSVSGYQDFYATDEVTINKGDVLAYTINVAGGSTYGY
                     AIWIDFNQDGTFQATEQVLTTSSYASSPKTGTVTIPNTAVVGSTRVRVLATFSPSNPS
                     DPCNNSGSGEYQDYAITIQQPVPVTYTMFTGKREGMVNMLSWQTATESNNIGFELQRS
                     SDGKQFSKLSFVNSKAINGNSTSNLSYGYNDVKPLAGTNYYRLKQVDKDGKFNYSTVV
                     ILKGDKVNQISIASVYPNPVRNTINVQIVSPANEKVSLVITDITGKVLMQENTSLTAG
                     DNVKQIDVSRLSQGAYLIKAICNNGCETAVFKFTKY"
     gene            1909..2535
                     /locus_tag="PLU36_07950"
     CDS             1909..2535
                     /locus_tag="PLU36_07950"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HMZ46719.1"
                     /translation="MKKWLEEVVLETQHIKLIPINENHKADLLNASTDGNLSELWVTS
                     VPTINNIDKYLAKAIEDYKQDKGLAFVVINKKSNSVIGTTRYTNATPEHRRLEIGYTC
                     YSKSFQKTHVNSECKLLLLTHAFEILKAIAVEFRTNWYNFPSRNAIARLGAKQDGVLR
                     NHQIMPDGSLRDTVVFSILESEWNTCKNALMYKIEQIHKKYPAIKDNL"
     gene            complement(2599..3813)
                     /locus_tag="PLU36_07955"
     CDS             complement(2599..3813)
                     /locus_tag="PLU36_07955"
                     /inference="COORDINATES: protein motif:HMM:NF013559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallopeptidase"
                     /protein_id="HMZ46720.1"
                     /translation="MKTYCKTLLLFMVLFANLIVVAQKKTAKRLGCNTADPINSFAFD
                     TTQPTRGMADNNFLWDNGKTINVKILSGSNALKTKVQKWAKEWMKYANIKFNFIDKGE
                     AQIRVFLGDKKGNYGHVTMGLGIRCLQRDANDFTMHFDTTDFVYEANDKAFRGTVLHE
                     FGHALGLIHEHMSPVSGIQWDSSAVYARKAMMGWNKDMTNAQLFRKYNISYTNGTQYD
                     PKSIMHYPISKWETKNRYWVDWNYDLSEGDKALIAALYPKGKRENKVPRITISNVGET
                     IIEYNHNRRGICIYPSFDITCADKEAEIYYVSLLIDENSYFLPTQSKDYQINKYAGLA
                     KAIKLPLNKVEKINKGTKNFELFIPDSELPSNIIGKKINVYFRVFLNNNGEFKDVFAN
                     ISSKGITINNLY"
     gene            complement(3926..4801)
                     /locus_tag="PLU36_07960"
     CDS             complement(3926..4801)
                     /locus_tag="PLU36_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46721.1"
                     /translation="MKRTLKILTLLLTSFNLFGQTSYSGFIGKYPIELVTNIYFDGDA
                     RAIYAYSNHDEPIVINGELKQGKLTLYEKDNNGKSKATITFDNFDTKSNQLEGIWKEQ
                     NSIKQLKISLTKNFDIDFGENIEWTAKEIIQPVSLKDKYFKLVISKAKDNFYANVTGV
                     KIFEKKTDKLIQQIDLECELRGLNNISIGDYNFDGIEDFSVFEHSAAGPNTSSLYFLF
                     NPKKRKHFKSSFKGISLEFDPKTKRIYEHNQCCGGRSHLNAEYRVVNNQMVLVKMTCI
                     EYDEKIQDFKKVKCE"
     gene            complement(4851..>5051)
                     /locus_tag="PLU36_07965"
     CDS             complement(4851..>5051)
                     /locus_tag="PLU36_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46722.1"
                     /translation="VYFGYQTARELFKINFEKVLIPEINNGQLIKIIRDQYFIDAKGY
                     NKIMGVPITKTELIEVISEMVK"
BASE COUNT         1725 a          811 c          747 g         1768 t
ORIGIN      
        1 ataaaagtac agctactaca ccattagtta gtaataacaa tatattttat ttaggcagtg
       61 caggtagtgg tgcacaaagt ataggttacc aaaccagtgc acaagccagt ttaacagctt
      121 ggcaaacagc aagcagccaa gatttaaata gtatggtagc agatccttta ttcacgaatc
      181 catctgtagg agattataaa cctacagaaa catcaataga taacaaagga gcttcgttag
      241 gtgtgcttcg tgatattttg gatactatcc gcccattaat acttcctgac ttgggagctt
      301 acgaatttgg cacaatgcct tcaccttgtt ctgcaacccc agttccaggt aatacagttt
      361 cttctgtgcc tattgcttgc cctaatagca attttacttt atctttagaa tttccaccat
      421 cagaattagg gcttacattt cagtggcaaa gtgccgatga tgcagcattt acaaccaatg
      481 tagttgattt aggaactgct tcaacgcaag ccacttcaca aacatctgct aaatactatc
      541 gttgtaaagt aacttgcact aattcagggt tatttgctta ctcaactcct atatttatag
      601 atattaattc atggattaat tgttattgta ctccagcaac aacaggaggc actacttatt
      661 atatttcagg ctttacaaca acaggaggta gtacaaatat taataaaaca acttctgctg
      721 gttcagtaag tggttatcaa gatttttatg caaccgatga ggtaactatt aacaaaggtg
      781 acgtacttgc ttatactatt aatgttgctg gtggtagcac ttatggctac gcaatttgga
      841 ttgattttaa tcaggatgga acattccaag ctacagaaca agtattaact acaagttcgt
      901 atgcttcttc tcctaaaact ggtactgtta caattccaaa tacagcagta gttggttcta
      961 caagagtgag agttttagca acattttctc ctagtaatcc ttcagaccct tgcaataatt
     1021 caggttcagg agagtatcag gattatgcaa ttactattca acaacctgta ccagtaacct
     1081 acactatgtt tacaggtaaa agagaaggca tggtaaatat gttatcatgg caaactgcta
     1141 cagaaagtaa taatattggt tttgaattac aacgtagtag cgatggtaaa caatttagta
     1201 aactaagttt tgtaaacagc aaagcaatta atggtaacag tacatctaat ttaagctatg
     1261 gctataacga tgtaaaacca ttagcaggta caaactatta tcgtttaaaa caagtagata
     1321 aagatggcaa gttcaactac tctactgtag ttatcttaaa aggcgataaa gtaaaccaaa
     1381 taagtattgc tagtgtttat ccaaaccctg taagaaatac cattaatgta caaatagtat
     1441 caccagctaa tgaaaaagta agtttagtga taacagatat tacaggtaag gtattaatgc
     1501 aagaaaacac aagtttaaca gcaggcgata atgtaaaaca aatagatgtt agcagactaa
     1561 gccagggagc atatttaata aaagcaatat gcaacaatgg ctgcgaaact gcagtattta
     1621 agtttacaaa atattaattg catacatatt cttgttataa aaaaattgag ccacttaatg
     1681 tggctcaatt ttttttaaga tatgtttcaa ataaaatatt ctcatttaaa acctaaaaaa
     1741 tataacataa taaaataatt gctaaccaat tgttacttac taaataattt tactatttct
     1801 attaatgaat agtagtataa aatattggtg gaaatgggta catttaacga cgattctgtt
     1861 attataataa caagattata gaaacaaaaa aattgaaaca aaaccaaaat gaaaaaatgg
     1921 ctcgaagaag ttgttttaga aacccaacac ataaagctta ttccaattaa tgaaaatcat
     1981 aaagcagatt tattaaatgc atctaccgat ggaaatttat ctgaattatg ggtaacatca
     2041 gtaccaacaa ttaataatat tgataaatat ttagcaaagg caattgaaga ttataaacaa
     2101 gacaagggat tggcttttgt agtaataaat aaaaaatcga atagtgtaat tggcactact
     2161 agatatacta acgcaacacc agaacataga agattagaaa ttggatatac ttgttattca
     2221 aaatcatttc aaaaaacaca tgtaaattca gaatgtaaat tactattatt gacacatgct
     2281 tttgaaatac ttaaagcaat agcagtagag tttagaacta attggtataa ttttccatca
     2341 agaaatgcta ttgcaagact tggtgctaaa caagatggag tattaagaaa tcatcaaatt
     2401 atgcctgatg gctctttaag agatactgtt gttttttcta tattggaaag tgaatggaat
     2461 acttgcaaaa atgcattaat gtataaaata gaacagattc ataaaaaata tccagctatt
     2521 aaagataatt tataatagct ggataaataa actcgtttta aatcttaatt ttttttgctg
     2581 agatataaaa agttatactt aatataaatt atttatagta atacctttag aggaaatgtt
     2641 ggcaaaaaca tctttaaact ctccgttatt gttcaagaat actctaaaat aaacattaat
     2701 ttttttccct attatattgc ttggcaactc actatcgggg ataaataatt caaaattttt
     2761 agtgccttta tttatttttt ctactttgtt tagaggaagt tttattgcct tagctaaacc
     2821 tgcatattta tttatttgat agtctttact ttgagtaggt aagaagtatg aattttcatc
     2881 aattaataaa cttacataat aaatttcagc ttctttatcg gcacaggtaa tatcaaaact
     2941 agggtaaatg caaatacctc ttctgttatg gttatattca attattgttt cgccaacgtt
     3001 tgaaatggtt atacgaggta ctttattttc tcttttacct ttagggtata atgctgcaat
     3061 taaagcttta tctccttcag ataagtcata attccaatca acccagtatc tatttttggt
     3121 ttcccattta gaaattgggt aatgcataat tgattttggg tcgtactgtg taccattggt
     3181 ataggatata ttatattttc taaaaagttg agcattcgtc atgtctttat tccaacccat
     3241 cattgctttt cttgcataaa cagccgaact gtcccattgt attccactta cagggctcat
     3301 gtgttcgtgt attaaaccta atgcatggcc aaactcgtgt aaaacagtac ctctaaatgc
     3361 tttatcgtta gcttcgtaaa caaaatcagt agtatcaaaa tgcatggtaa aatcattcgc
     3421 atctctttgt aagcatctta ttcctaagcc cattgttaca tgcccataat tccctttttt
     3481 gtcgcctaaa aaaactctta tttgagcttc tcctttgtca ataaagttaa atttaatatt
     3541 tgcatatttc atccactcct tagcccattt ttgcacttta gtttttaaag cattgctacc
     3601 acttaatatt tttacattta ttgttttacc attatcccac aaaaaattat tatctgccat
     3661 acctcttgta ggttgggtag tatcaaaagc aaaactatta attggatcgg cagtattgca
     3721 acctaaacgt ttggctgttt ttttttgagc aacaacaata aggtttgcaa aaagcaccat
     3781 aaataataaa agtgttttac aatatgtttt cataaagtgt aatttaataa acctaaagtt
     3841 aatcatgttt tatgaaaaat ttgtacccat tgaaaggtat tttataattt aacggattgg
     3901 agaaactcaa tgttataggc tgtttttatt cgcattttac ttttttaaaa tcttgtattt
     3961 tttcatcata ctctatacaa gtcatcttca ctaataccat ttggttgttt acaactctgt
     4021 attctgcgtt caaatggctt cgtcctccac aacattgatt atgttcgtaa atacgttttg
     4081 tcttagggtc aaactccaat gaaattcctt taaatgaact cttaaaatgt tttcttttct
     4141 tagggttgaa aagaaaatat aaacttgagg tgtttggtcc agctgcacta tgttcaaata
     4201 cagaaaagtc ctcaatgcca tcaaaattgt aatctccgat acttatattg tttagtcccc
     4261 taagttcaca ttctaaatca atttgctgaa ttagcttatc tgtctttttc tcgaatattt
     4321 ttacgccagt cacatttgca taaaaattgt cttttgcctt agaaattacc aatttgaaat
     4381 acttgtcttt taaagaaaca ggctgtataa tttcttttgc tgtccactca atgttttcac
     4441 cgaagtcaat gtcaaaattt tttgtcaaac taattttgag ttgcttgatt gaattttgtt
     4501 ctttccaaat tccttctaat tgattgcttt ttgtgtcaaa attgtcaaag gttattgtag
     4561 ctttgctctt tccattattg tctttttcgt aaagtgtcaa cttaccttgt ttaagttcac
     4621 cattaattac aataggttcg tcatggttag agtatgcata aattgctctt gcatctccat
     4681 caaagtatat gtttgttact aattcaattg gatatttacc aataaatcca gaataacttg
     4741 tctgtccaaa taagttaaac gaagtcagaa gaagtgtcaa tattttaagt gttcgtttca
     4801 ttttgtgttt tgtcctaaaa taggtggcaa ctaagacaat aaacgaataa tcattttacc
     4861 atttcactaa ttacctctat tagttcagtt ttagtaattg gcacacccat tattttatta
     4921 tatcctttag catctataaa atattggtct ctaataattt ttatcaattg cccattgttt
     4981 atttctggaa ttaaaacttt ttcaaaatta attttaaaaa gttctctggc agtttggtaa
     5041 ccaaaataaa c
//
LOCUS       DAOQPK010000162         5048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_10273_length_5048_cov_3.910475, whole genome shotgun sequence.
ACCESSION   DAOQPK010000162 DAOQPK010000000
VERSION     DAOQPK010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10273_length_5048_cov_3.910475"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PLU36_07970"
     CDS             complement(<1..317)
                     /locus_tag="PLU36_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HMZ46723.1"
                     /translation="MDLQSIKNRFGIIGNSTELNHALNTAVQVSATDLTVLIVGESGV
                     GKEIFSQIIHALSIRKHNNFIAVNCGAIPEGTIDSELFGHEKGSFTGAVESRKGYFET
                     VNGG"
     gene            complement(335..1756)
                     /gene="miaB"
                     /locus_tag="PLU36_07975"
     CDS             complement(335..1756)
                     /gene="miaB"
                     /locus_tag="PLU36_07975"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HMZ46724.1"
                     /translation="METLTLVDKTHDEARQGEAFAPFKLDNNIYKKYFYIESYGCQMN
                     FSDSEIVASILHAEGFGATKDITIADLILINTCSIREKAEQTIRKRLTEFRKLKHAKP
                     GLLIGVLGCMAERIKAKLLEEEKLVDLVVGPDAYRSLPSLVEEAETGQKAINVLLSRE
                     ETYADISPIRLDSNGVTAFVSIMRGCNNMCSFCVVPFTRGRERSREVDSILAEAKALF
                     EQGFKEVTLLGQNVDSYYWVNPINNEVVTFAMLLEKVALISPDLRVRFSTSHPKDITD
                     DVLNTIAKYENICKYIHLPVQSGNTRILQLMNRTYTKEWYLAKVDRIKELIPECAIST
                     DIITGFCTETEEEHKDTLSVMKHSQYDFAYMFFYSERPGTLAKKRYQDDIPEETKKRR
                     LAEVVELQNELSAKSNLKDIGKTFKVLIENDSKKSNLHWMGRNSQNKVIVFPKENFNL
                     QKGDYVNVKVNSCTQATLIGEKV"
     gene            1907..2851
                     /locus_tag="PLU36_07980"
     CDS             1907..2851
                     /locus_tag="PLU36_07980"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HMZ46725.1"
                     /translation="MKNWLFISFFLTFCGLNAQEIVVKRISGDIFKSVKKEADTSKWK
                     WKRNGIVGVNIAQGALSNWAAGGDNFSMSINTNFSYYIYHRAKRYTWDNSFDFNFGLV
                     QATTLGSRKNDDRIDLLSKFGYKVDTLNKWYASMLFNFRSQLFDGYNYSGSSSTFSSS
                     FLSPAYIILSVGMDYKPSPKFSLFVSPLTSRWVIVANDYLSKKGAYGVPINKHSVGEL
                     GAYVTMSYVNEIATNISYRGRMDLFSNYKNNPQNVDIFFTNMFSFRINKLFSATYNLD
                     LIYDDDIKIFGSNKNAPRLQLKSLIGIGFLMRLEPKKI"
     gene            complement(2859..3161)
                     /locus_tag="PLU36_07985"
     CDS             complement(2859..3161)
                     /locus_tag="PLU36_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HMZ46726.1"
                     /translation="MLLHSKTNSNSKEQVDVDVLEISISPFQLIVWNDEVNTFEWVIK
                     SLIEVCNHTEEQAEQCAFLIHFKGKYAVKNGDYDTLKPMCNSITERGINATIEQLN"
     gene            3307..3381
                     /locus_tag="PLU36_07990"
     tRNA            3307..3381
                     /locus_tag="PLU36_07990"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3342..3344,aa:Val,seq:tac)
     gene            3488..4153
                     /gene="aat"
                     /locus_tag="PLU36_07995"
     CDS             3488..4153
                     /gene="aat"
                     /locus_tag="PLU36_07995"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HMZ46727.1"
                     /translation="MAITLLTEKLWFPPVETASLDGLLAIGGDLQPERILLAYQSGIF
                     PWYDGDLPLWWFPNPRFVLLPNELKISKSMQQLIKQNKFKVTVNNAFSEVIKNCKNCI
                     RKDQEGTWINEEVVNAYTQLHKMGYAHSVEAWQNNELVGGLYGIMMGQVFFGESMFSL
                     KSNASKYAFISYVQQLAKEGVALIDCQVYTKHLESLGAKMIMKEQFSQLLKHFLHSTQ
                     INK"
     gene            complement(4156..4446)
                     /gene="gatC"
                     /locus_tag="PLU36_08000"
     CDS             complement(4156..4446)
                     /gene="gatC"
                     /locus_tag="PLU36_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HMZ46728.1"
                     /translation="MNVTTELVDKLANLTMLQFNEEEKKDIQNDLQSMISFVEKLQEL
                     DTNGVEPLQHISSNNNVLREDNIKPSISKEEAFLNAPLQHENYFAVSKIIKK"
     gene            4564..>5048
                     /locus_tag="PLU36_08005"
     CDS             4564..>5048
                     /locus_tag="PLU36_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HMZ46729.1"
                     /translation="MSEEKSLNFIEEIIEKDLQDGKHETILTRFPPEPNGYLHIGHAK
                     SICLNFGLALKYNGKTNLRFDDTNPVTEETEYVESIKEDINWLGFKWTNEFYASDYFD
                     KLYEFAIILIKKGLAYVDDSSSEEIAQQKGTPTIPGIRNRFSERDIEENVKLFTEMKD
                     G"
BASE COUNT         1667 a          762 c          731 g         1888 t
ORIGIN      
        1 ccaccattca ctgtttcaaa ataccctttt ctgctttcta cagctcctgt aaagcttccc
       61 ttctcatgcc caaaaagttc actatcaata gttccttcag gtatggcacc acagtttact
      121 gctataaaat tattatgttt tcttatagat aaagcatgaa ttatttgaga aaaaatttct
      181 ttaccaactc cgctttcacc tacaattaaa accgttaaat ctgttgccga aacttgtaca
      241 gcagtattta atgcatggtt taattctgta gagttgccta taataccaaa cctatttttt
      301 atactttgta aatccactat tttctttttt agggttaaac tttttctcct attaatgtag
      361 cttgtgtgca actatttact ttcacattta cataatctcc tttttgtaag ttgaaatttt
      421 cttttggaaa aacaattact ttgttttgag aatttctacc catccagtgt aaattacttt
      481 tcttactatc gttttctatt aaaactttaa atgttttacc aatatctttt aaattacttt
      541 tagcagatag ttcattttgc aattctacca cttctgcaag tcttcttttt ttagtttctt
      601 ctggtatatc atcttggtac ctttttttag ctaaagttcc gggtctttcg ctgtaaaaaa
      661 acatatatgc aaaatcgtat tggctatgct tcattacact taatgtatct ttatgttctt
      721 cttcagtttc tgtacaaaag cctgtaatta tatctgtact aattgcacat tctggtatta
      781 attctttaat tctatccact tttgctaaat accattcttt tgtataagtt ctgttcatta
      841 attgtaatat tctggtattg ccactttgta caggcaaatg aatatacttg caaatatttt
      901 cgtatttagc aatggtgttt aaaacatcat ctgtaatgtc tttagggtga gaggtgctaa
      961 acctaactct taaatcaggg ctaattaaag ctactttttc taataacatg gcaaatgtta
     1021 ccacttcatt atttattggg tttacccaat aatagctatc tacattttgg cctaataagg
     1081 taacttcttt aaagccttgt tcaaataatg cttttgcttc ggctaaaata gaatctactt
     1141 ctctgcttct ttctctgccc ctagtaaatg gtactacaca aaagctacac atattattac
     1201 agcccctcat tattgataca aaggcagtaa caccattact atctaaacga ataggagaaa
     1261 tatctgcata agtttcttct ctgcttaata aaacgtttat tgctttttgt cctgtttctg
     1321 cttcttcaac taatgaaggt aagcttctgt aggcatctgg tcctacaact aaatcaacta
     1381 atttttcttc ttctaataat ttggctttaa ttctttctgc catacaacct aaaacaccaa
     1441 ttaataaacc tggttttgca tgttttaatt ttctaaactc agtaagcctt ttcctaattg
     1501 tttgttctgc tttttctcta atagagcagg tgttaattaa tattaagtcg gctattgtaa
     1561 tatcttttgt ggcaccaaaa ccttctgcat gtaaaataga agctacaatt tcactatcac
     1621 taaagttcat ttggcagcca tagctttcaa tataaaaata ttttttgtaa atgttattat
     1681 caagtttaaa aggtgcaaaa gcttcccctt gtcttgcttc atcatgtgtt ttatctacta
     1741 gcgttaaagt ttccattaaa attaaatact tatttaagct gcaaaagtag caaaaaatat
     1801 tactattgcc aatttgacag actattaaca gaatttaaat tggctgatat tatttttgta
     1861 aaaaatattt ttacattcat tcatgaaaag accttgttgc aaatacatga aaaattggtt
     1921 atttatttca ttttttttaa cattttgtgg cctaaatgct caagaaattg tagttaaaag
     1981 aatatctgga gatatattta aaagtgtaaa aaaagaagct gatacaagta aatggaaatg
     2041 gaaacgaaat ggtattgttg gagttaatat tgcacaagga gcattaagca attgggctgc
     2101 aggtggcgat aacttttcga tgtcaataaa tacaaatttt agttattata tttatcatag
     2161 agctaaaaga tatacatggg ataatagttt tgattttaat tttggcttag ttcaagctac
     2221 tactttagga agtagaaaaa atgatgatag aattgactta ctttcaaaat ttggatataa
     2281 agtagatact ttaaataaat ggtatgcttc tatgttattt aactttagaa gccaattgtt
     2341 tgatggctac aactattcag gtagtagttc aactttttca tcttcctttt tatcgcctgc
     2401 atatattatc ttatctgttg gtatggatta caaaccttct ccaaaattct cactttttgt
     2461 ttcaccatta acgtcaagat gggttattgt tgcaaatgat tatttatcca aaaaaggtgc
     2521 ttatggtgtg cctataaaca aacattctgt tggtgaattg ggggcttatg taaccatgag
     2581 ctatgtcaac gaaattgcaa ccaatatttc atatagaggt agaatggatc tattttctaa
     2641 ctataaaaat aatcctcaaa atgtagatat cttttttact aatatgttct cttttagaat
     2701 caataaatta ttttcagcaa cctataattt ggatttaatc tatgatgatg atattaaaat
     2761 ttttggttct aataaaaatg ctccacgttt acaattgaaa agtttaattg gtattggctt
     2821 tttaatgagg ctggagccta aaaaaatcta aaataggctt aatttaactg ctctatggtg
     2881 gcattaattc ctctttcagt aatagagtta cacataggct ttaacgtatc ataatcgcca
     2941 ttttttactg catattttcc tttaaaatga attaaaaaag cacattgttc agcttgctct
     3001 tctgtatggt tacaaacttc aattaaagat tttattaccc attcaaaagt atttacttca
     3061 tcattccaaa caattaattg aaaaggtgaa atacttattt caagtacatc cacatctacc
     3121 tgctcttttg aattactgtt tgtttttgaa tgcagcaaca tgcataattg gttttaaaaa
     3181 ataaaactac aaattagcaa caaataaaat aatattaagt aaaagaagaa atatttttta
     3241 aaaactgaaa ttttttttgg ctaatatttt attgcaccta tttttgcaat cccaaattaa
     3301 aaatatggga gcttagctca gctggtttag agcatctgcc ttacaagcag agggtcggcg
     3361 gttcgaaccc gtcagctccc acaaaaacct acaaattttg taggtttttt ttatgaaata
     3421 aattttcttt gttgttagct ttctgatttt atctttccta tttgatcaac tagtttaata
     3481 attacatttg gctataacat tacttacaga aaaactttgg tttcctccgg tagaaacagc
     3541 ttctttagat ggattattag caattggcgg agatttacag cccgaaagga ttttacttgc
     3601 ttatcaatct ggtatttttc cttggtacga tggtgattta ccactttggt ggtttcctaa
     3661 cccaagattt gttttattac ccaatgagct aaaaatcagt aaaagcatgc agcaattaat
     3721 taagcaaaat aaatttaagg ttactgtaaa caatgcattt tctgaagtaa ttaaaaactg
     3781 taaaaattgt attagaaaag atcaagaagg tacttggata aatgaagaag tggtaaatgc
     3841 ttatactcaa ttacataaaa tgggctatgc acattcggta gaagcttggc aaaataatga
     3901 gttagtagga gggttatatg gcataatgat ggggcaagtt ttttttggtg aaagtatgtt
     3961 tagcttaaaa tctaatgcca gtaaatatgc atttataagt tatgtacagc aacttgcaaa
     4021 agaaggtgta gctttaatag attgccaggt atatacaaag catttagaaa gtttaggagc
     4081 aaaaatgatt atgaaagaac agttttcgca attgcttaaa cattttctac acagtacaca
     4141 aataaataag taaccttatt ttttaattat tttagaaaca gcaaaataat tttcatgttg
     4201 taatggtgca tttaaaaatg cctcttcttt actaatactt ggtttaatat tatcttctct
     4261 taaaacatta ttattgctgc ttatgtgttg caatggttca actccatttg tatctaattc
     4321 ttgtagtttt tcaacaaaag aaatcatgct ttgcaaatca ttttgaatat cttttttttc
     4381 ttcctcatta aattgcaaca ttgtaaggtt ggctaactta tccactaatt cagttgtaac
     4441 attcataact acaaaaataa ttaattcttt gatttttaat tttattgatt ttttttagtt
     4501 gaattgtttt ttgttttcac tattatctaa agcgtatttt tgccagctac taaaataaat
     4561 agtatgagtg aagaaaagag tttaaatttt attgaagaaa ttattgaaaa agatttacaa
     4621 gatggaaagc atgaaactat tcttacacgt tttccgccag aaccgaatgg gtacttacat
     4681 attggccatg ccaaaagtat ttgtttaaat tttggtctag cattaaaata taatggtaaa
     4741 actaacttgc gttttgatga tacaaaccct gtaacagaag aaacagaata tgtagaaagt
     4801 attaaagaag atattaactg gttaggcttt aagtggacaa atgaatttta tgcatctgat
     4861 tattttgata aattatatga gtttgcaatt attttaataa aaaaaggatt agcttatgta
     4921 gatgatagca gcagcgaaga aattgctcag caaaaaggta ctccaacaat acctggtata
     4981 agaaatagat tttcagaaag agatatagaa gaaaatgtaa aattatttac cgaaatgaaa
     5041 gacggtaa
//
LOCUS       DAOQPK010000163         4985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_10459_length_4985_cov_4.904260, whole genome shotgun sequence.
ACCESSION   DAOQPK010000163 DAOQPK010000000
VERSION     DAOQPK010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10459_length_4985_cov_4.904260"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..515
                     /locus_tag="PLU36_08010"
     CDS             123..515
                     /locus_tag="PLU36_08010"
                     /inference="COORDINATES: protein motif:HMM:NF024093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMZ46730.1"
                     /translation="MQPYFGVEIILYVANQEESKQFYSNLLQLQPILHVPGMTQFTIT
                     ENCSLGLMPNTGIAKIITPAMPHPDQAIGVPKCEVYLYVSNVQFYYEHALAIGAKLVS
                     KLDDRDWGDKACYFADLDGHIIAFAEKL"
     gene            complement(541..1854)
                     /locus_tag="PLU36_08015"
     CDS             complement(541..1854)
                     /locus_tag="PLU36_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HMZ46731.1"
                     /translation="MKSKAPLLFIITAIASLITAISFGIIATLQYVQFDFLKPFLTFN
                     KVRPMHVTGALSWVLLSAIGGIYYYLQKDGLLKKQLLVSYHFIIFLLTGIGIYTCYAL
                     GIFGGKEYLEFPPILIIPILIGWFLFAYNVIGSLLNGFTKRPVYFWMWCTGSVFMIYH
                     LSEAYIWLTPYFKDKFLANTALQWKAGGSFVGSWNMLVYGTATYVMAKLSPTSGVGTD
                     KKSFFFYFLGLSNLMFGWAHHVYLIPIAPWIRYFAYLVSMTEWILLISIIYSWKKSLN
                     FNTTIENKVVIKFLSSTDKWVLINLFVALLISIPAINYYTHGTHVTVAHSMGTTIGIN
                     TTILLASIAYIVQKENPNFNFKLVLKGLTFFNIALILFLITLLVAGASRSQWMYALEP
                     SAFAAMQYKLKTVYLIMAILGSLLTTTILVMVLPLFKGLMKALRG"
     gene            complement(1854..2258)
                     /locus_tag="PLU36_08020"
     CDS             complement(1854..2258)
                     /locus_tag="PLU36_08020"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HMZ46732.1"
                     /translation="MSKKFLILIVLICFFGVYNYLVYTQSQPPTKPLLSTNALQGQKL
                     WQDNNCNSCHQFYGLGGYLGPDLTNVYSLKGGDFINAMLNSGISTMPLFHFSDLEKQN
                     IITFLKEVDSTGFYPDKKSIIHKNGWVEISEK"
     gene            complement(2355..2720)
                     /locus_tag="PLU36_08025"
     CDS             complement(2355..2720)
                     /locus_tag="PLU36_08025"
                     /inference="COORDINATES: protein motif:HMM:NF013331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HMZ46733.1"
                     /translation="MKQDISNNSDIEKVVSVFYERVKNDDLLGHFFTGVMNVNWDKHF
                     VMMCSFWENVLFYTGDYEGDPLNVHKTIHSKEPTTTVHFERWMKLFNATVDEFFEGKN
                     AEKMKMHAKSISSVMLSKI"
     gene            complement(2748..3515)
                     /locus_tag="PLU36_08030"
     CDS             complement(2748..3515)
                     /locus_tag="PLU36_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HMZ46734.1"
                     /translation="MIRVLKYVALDILKNKIIIAYTIMLALFAWSAFSLEDNESKGVL
                     TVLNIVLLTVPLVSILFSTIYIYNSSEFIELLISNPVNRGMVWKALFIGLSLSMVTAY
                     IVGVGIPLLLFANFATAIMMVATGIMLSMIFVSVAFLCSMFTRDKAKGIGVSIMLWLY
                     FALLFDGIVLFLFFQFADYPIEKIVVILSALSPVDLSRILILLKLDVSAMLGFSGAIF
                     QNYFGSNWGLVLSFALLGLWIAVPFAISLRKFKKKDL"
     gene            complement(3512..4222)
                     /locus_tag="PLU36_08035"
     CDS             complement(3512..4222)
                     /locus_tag="PLU36_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ46735.1"
                     /translation="MIKIENITKHFGRFRALNNLSIELNKGECIALIGPNGCGKTTLI
                     KSILGMVLPELGTIKFDGKSIKNDNEYRKHIGYMPQIGRYPENMTIGQVIEMIKGIRN
                     HTNDLDTELYEKFEIEKMLNKKMRTLSGGTTQKVSAVLAFMFQSKVFILDEPTAGLDP
                     ISSEILKNKIIDAKNKGCLILITSHLLSELDDLVTQIVFMQEGKLILHENVEILKEKT
                     GEATIAKSIIHLLKTQRA"
     gene            complement(4219..>4985)
                     /locus_tag="PLU36_08040"
     CDS             complement(4219..>4985)
                     /locus_tag="PLU36_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HMZ46736.1"
                     /translation="SGNGIHCWKSDSLQIIGNRVYGHRDGIYFEFVTHTVIWRNIAHK
                     NLRYGLHFMFSHKNAYFTNFFKDNGAGVAVMFTKEVVMMNNTFQENWGDAAYGVLFKE
                     LSDCYLSGNKFIRNTTGIFFEGSNRIVAEKNTFINNGWGMRLQANCIDNRIDKNNFLG
                     NTYDISTNGFLTLNHFDANYWDKYEGYDLNKDNIGDVPFRPLSLFSVIVEKNSPAMLL
                     YRSFMISLLDKSEKILPTLTPENFIDNNPRMTPYKL"
BASE COUNT         1798 a          849 c          631 g         1707 t
ORIGIN      
        1 attagtattg gggttggcgg gtttaattgc tggtaatgaa atgctttttg ctgttgcagt
       61 aattttactt tctagttcta ttatgttatt tgcatttcaa aaaaaaaata acaaaatata
      121 gtatgcaacc ttattttggt gtagaaataa ttttatatgt agctaatcaa gaagaaagta
      181 aacaatttta tagcaacctc cttcaattac aacctatact tcatgtgcca ggaatgaccc
      241 aatttacaat tactgaaaat tgttcacttg gtttaatgcc caatactgga attgcaaaaa
      301 ttattacgcc agcaatgcct catcctgatc aagcaattgg tgtaccaaaa tgcgaagtat
      361 atttatatgt aagtaacgtt caattttatt atgaacatgc tcttgcaata ggagcaaaac
      421 ttgtaagcaa gttagatgac agagactggg gagataaggc ctgttatttt gcagatttag
      481 atgggcacat tattgctttt gctgaaaaat tataaccacc catcctcaac aaataaattt
      541 ctatcctctt agtgccttca ttaatccttt aaataatggc agcaccatta ctaaaattgt
      601 agtagttaat aatgaaccta aaattgccat tattaaataa acagttttta gtttgtattg
      661 catggcagca aatgcagatg gctctaaagc gtacatccat tggcttcttg atgctcctgc
      721 aactaaaagt gtaattaaaa atagaataag ggcaatatta aaaaaggtta agccttttaa
      781 aactaattta aaattaaaat tggggttttc tttttgtaca atataagcaa tagatgctaa
      841 taatatagtg gtattaattc caattgttgt gcccatacta tgagcaacag ttacatgtgt
      901 accatgcgta taataattta tagcaggtat agaaattaac agagcaacaa ataaattaat
      961 tagtacccat ttatctgtag aggataagaa cttaattaca actttgtttt cgatggttgt
     1021 attaaaattc agcgatttct tccaagagta aataatgctg ataagcaata tccattcagt
     1081 catacttact aaatatgcaa aatatcttat ccaaggtgca ataggaatta agtaaacatg
     1141 atgtgcccag ccaaacataa gattggataa gcctaagaaa taaaagaaaa aagatttctt
     1201 atcagtacca acgccacttg ttggactaag ttttgccata acataagttg cagtaccata
     1261 cactaacata ttccaagaac ctacaaatga tcctccagcc ttccattgta aagctgtatt
     1321 agctaaaaat ttatctttaa aatatggtgt taaccaaata taagcttccg ataaatgata
     1381 aatcataaat accgaacctg tacaccacat ccaaaaataa actggtcgtt tagtaaaccc
     1441 atttaataaa gaccctatta cattgtatgc aaacaaaaac caaccaatta aaataggaat
     1501 gatgagtatt ggaggaaact ctaaatattc tttacctcca aaaattccca aagcataaca
     1561 ggtataaatt ccaattccag taagtagaaa aataataaaa tgataactaa ctaaaagttg
     1621 tttttttaat agcccatctt tttgtaaata gtaataaatt cccccaatag cagaaagtaa
     1681 aacccaactt aatgcaccag ttacatgcat gggtctaact ttgttgaatg ttaaaaaagg
     1741 ttttaaaaaa tcaaactgaa catattgtaa tgttgcaata attccgaatg aaatagcagt
     1801 tattaaagat gctattgctg taatgataaa taataaaggt gcttttgatt tcattatttt
     1861 tctgatattt ctacccaacc gtttttatga ataatacttt ttttatcagg ataaaatcct
     1921 gtgctatcta cttcttttaa gaatgtaata atattttgtt tttccaggtc tgaaaagtgg
     1981 aataatggca ttgtacttat tccgctattt aacattgcat tgataaaatc tcctcctttt
     2041 aaagaatata catttgtaag atctggccct aaataaccac ctaacccata aaattgatga
     2101 caggaattgc agttattatc ttgccacaat ttttgccctt gtaaagcatt tgtagatagc
     2161 aatggctttg ttggaggctg actttgagta taaaccaaat aattatatac tccaaaaaaa
     2221 caaattaata caattaaaat tagaaatttt ttagacattt tttgaatcaa aaattatgga
     2281 caaatttaat ttttcaatgt gcatttaatc atgacctgta tcataagaaa actataactt
     2341 ataatgaaca acaactaaat tttgctgagc attacactag atatagactt agcatgcatt
     2401 ttcatttttt ctgcattctt tccttcaaaa aactcatcca cagttgcatt aaataatttc
     2461 atccatcttt caaaatgtac agttgtggtt ggctcttttg aatgaatagt tttatgaaca
     2521 ttcaatgggt ctccttcata atctcctgta taaaaaagca cattctccca aaaagagcac
     2581 atcattacaa aatgtttatc ccaattaaca ttcataacac cagtaaaaaa atgacccaat
     2641 aaatcatcat tttttactct ttcgtaaaaa acacttacta ctttttcaat atcgctgtta
     2701 ttacttatat cttgtttcat ttattattat ttaaacaatt aataatttta caaatctttc
     2761 tttttaaatt ttcttaatga aattgcgaat ggaactgcaa tccataatcc taataaagca
     2821 aaggataaaa ccaagcccca attactacca aaatagtttt gaaaaatagc tcccgaaaag
     2881 ccaagcattg cagatacatc taatttaaga agaattaaaa ttctacttaa atctacaggg
     2941 cttaaagcac taagaattac cacaattttt tctataggat aatcggcaaa ctgaaaaaat
     3001 aaaaacaaaa ctatcccatc aaacaacaaa gcaaagtaca gccatagcat gatagaaact
     3061 cctatgcctt ttgccttatc tcttgtaaac atactacata aaaatgctac agaaacaaat
     3121 atcatagaaa gcatgatgcc tgttgcaacc atcattattg ctgttgcaaa atttgcaaat
     3181 agtaataatg gaatgccaac gccaacaata taagctgtta ccatacttaa tgataagcca
     3241 ataaataaag ctttccaaac catgcctcta ttaacagggt tgctaattaa taattctata
     3301 aactcgctac tattataaat gtaaatggta gaaaataata tggaaaccaa tggaacagta
     3361 agtaaaacaa tatttaacac tgttaataca cctttagatt cattatcttc taaactaaaa
     3421 gcactccatg caaacagagc aagcataatt gtataagcaa ttataatttt attttttaaa
     3481 atatctagtg caacatattt taaaactctt atcatgccct ttgtgttttt aaaaggtgaa
     3541 taatagattt tgctattgta gcttctccgg ttttttcttt taatatttct acattttcat
     3601 gtaatattaa ttttccttct tgcataaaaa ctatctgagt aactaaatca tctaactcgc
     3661 ttaataaatg agatgtaatg agaattaagc agcctttatt ttttgcatct ataattttat
     3721 tttttagtat ttcggaggat attggatcta aacctgcagt tggctcatct aaaataaata
     3781 ctttggattg aaacataaaa gccaacactg cactcacttt ttgtgtagta ccaccactta
     3841 aggttctcat ttttttattc aacattttct ctatctcaaa cttttcatac aattcagtat
     3901 ccaaatcatt tgtatgattt ctaatgcctt taatcatttc tataacttgc cctatagtca
     3961 tattttcagg gtaacgacca atttgtggca tataaccaat atgttttctg tactcattat
     4021 cgttttttat gcttttacca tcaaatttta ttgtacctaa ttctggtaaa accattccta
     4081 aaattgattt tattaatgtt gttttaccgc aaccatttgg gccaattagt gcaatacatt
     4141 cccctttatt taactctata cttaaattat ttaatgccct aaacctgcca aaatgttttg
     4201 ttatgttctc aattttaatc atagtttgta gggtgtcatt cttgggttat tatcaataaa
     4261 attttctggt gttaaagtag gtaaaatttt ttctgactta tctaaaagag atatcataaa
     4321 acttctgtaa agcaacattg ctggtgaatt tttttcaaca atcaccgaaa ataaactcaa
     4381 tggcctaaat ggcacatcgc caatattatc tttattcaag tcatatcctt catacttatc
     4441 ccaatagttt gcatcaaaat gatttagcgt taaaaaacca ttagtagaaa tatcatacgt
     4501 attgcctaaa aagttattct tatcaattct attatctata caatttgctt gcaaacgcat
     4561 tccccaacca ttatttataa aagtgttttt ttctgcaaca attctgtttg agccttcaaa
     4621 aaaaatacct gtagtgttac gaataaattt atttcctgat aaataacaat cggataattc
     4681 tttaaataaa actccataag ctgcatcgcc ccaattttct tgaaaagtgt tattcatcat
     4741 taccacttct ttagtaaaca taacggctac ccctgctcca ttatctttaa aaaaattggt
     4801 aaagtaggca tttttatgag agaacataaa atgcaaacca tatcgtaaat ttttatgggc
     4861 aatattcctc caaattactg tgtgtgtaac aaattcaaaa taaattccat ctctgtggcc
     4921 atacactcta ttaccaatta tttgcaaaga atcactcttc caacaatgaa ttccattacc
     4981 cgatt
//
LOCUS       DAOQPK010000164         4979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_10485_length_4979_cov_4.597482, whole genome shotgun sequence.
ACCESSION   DAOQPK010000164 DAOQPK010000000
VERSION     DAOQPK010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10485_length_4979_cov_4.597482"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..789
                     /locus_tag="PLU36_08045"
     CDS             64..789
                     /locus_tag="PLU36_08045"
                     /inference="COORDINATES: protein motif:HMM:NF023812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3658 domain-containing protein"
                     /protein_id="HMZ46737.1"
                     /translation="MKDDYAVGPLYNIYESEGYQQRREWWKNLLENSPYKEQLDIVDD
                     KLTVHNLIKKLEENTEEKVWIWAGQNQHDVCGYYWLMSQLKNFQSRIFILYLNNLPFI
                     NEKGQIFYPQHLSEIQPKEFLKAKKLAREITLSEFELDPDEWKKLSNENAMVRILEGG
                     KKIVSKEENFYDNEILSAINNEPQKLNKILLHILNKMKIKTGDVFLVWRINELAKQMK
                     IEISGDWNKGWKEMEMSLLKTPQ"
     gene            820..3321
                     /locus_tag="PLU36_08050"
     CDS             820..3321
                     /locus_tag="PLU36_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HMZ46738.1"
                     /translation="MMSDTNNNESGVQLQFKNWFLDYASYVILERAVPAIEDGLKPVQ
                     RRILHAMKEMDDGRFNKVANIIGQAMQYHPHGDASIGDALVNMGQKDLLIETQGNWGD
                     IRTGDDAAAPRYIEARLSKLALEIAFNNKTTNWQLSYDGRKNEPIVLPMKFPLLLAQG
                     AEGIAVGLSTKILPHNFIEIIEASIKYLKGKKFELYPDFQTGGLIDVSNYNEGRRGGK
                     IKVRCIIEEIDKKSLVIKDVPFGVTVAQLCESITKANDSGKIKIKKLTEFTGKNVEIQ
                     IDLAAGISSDITIDALYAFTDCEVSISPNACIIENNKPKFVGVQELLKISVDETKMLL
                     QKELQIKLAELEDKWHYTSLEKIFFEEKIYKELEQKYASWELVLQAIENAFQPFIKKL
                     KRKSISREDVLKLTEKPVRRIYKLDIDDLIEQIKAIDAEIVQVNNHLEHLTDFTITYF
                     ENLQKKFAKGRERKSEIKLFDTIKVQHITIANTKLYVNREEGFIGTSLKKDELLFECS
                     DLDDIIVFTKRGIMKVVKVGDKVFIGKDIIHAAIFKKNDERTTYNMIYVDAKSGISYA
                     KRFNVTGITRDKEYDLTKGSDKSKVHYFSSNANGEAEMVKIILSPNCTARNKELEFYF
                     EELEIKGRSSQGNIVTKYAVKSIKFKEAGRSTLSGIKLWFDDIYGRLNTEEKGQYLGM
                     FEDDKLLLIYKDGYYEITDTELTQRLDGDKILLIEKFNPQKIITAIYLDNDKQQFNVK
                     RFYIETTTLKSKFLFIKEGDGNYLETITTATEPILQVQTGRGQQVRKAKFKIDKMVEV
                     MGWKAIGAKLMDFSKTVEMEWIQVPKVENNQTELF"
     gene            complement(3390..3956)
                     /locus_tag="PLU36_08055"
     CDS             complement(3390..3956)
                     /locus_tag="PLU36_08055"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HMZ46739.1"
                     /translation="MKKVLFSALAIACFTALYSFVPGIKTLVTDDYKVNTEHSKIDWV
                     GSKKEGYHPGTFMLKSGNVNVENGRITKGQFVIDMANLKVTDKAGVKLEGHLKADDFF
                     DVTKHPEAIFEIENVNYTSETAVELTGTLTIKGIKVPIKFPGIVRGVSDKKLFAQAFF
                     SLDSRLLPITDKYAASDVQIMVYLYANK"
     gene            complement(4114..>4979)
                     /locus_tag="PLU36_08060"
     CDS             complement(4114..>4979)
                     /locus_tag="PLU36_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HMZ46740.1"
                     /translation="QLTETQLVNVDRLDFLKNQLLFFIGSLVVLFSSFYALLFYIPFA
                     KYRVYIYSILFTLTAFVYFKAKDYYAIGIYPIYIAFGSVYLSKILEGGWKKYLQPIAI
                     IIPLLLFIPIYNVAFPNKSPEYIVKHSENYKKLGLLRWEDGKDHLIPQDFADMLGWKE
                     LASKVDSIYLSLPNKQNTLILCDNYGQAGAINYYTKQKLQAVSFNADYINWIDLSNKY
                     VNLIRVKEAEEVKEELQKISPYFKNSLLAGSITNPYAREYGTGIFAFIGAKIDIRERL
                     GEEIDKNKNYH"
BASE COUNT         1921 a          631 c          831 g         1596 t
ORIGIN      
        1 cttctctctt ttctcttctt tgtttatttg aaggaattat tttattatat tttaaaataa
       61 gaaataaaag atgattatgc tgttgggcca ttgtataata tttatgaatc agaaggttat
      121 cagcaaagaa gagaatggtg gaagaattta ttagaaaact caccttacaa agagcaatta
      181 gatattgttg atgataaatt aacagttcac aaccttataa aaaaattaga agaaaataca
      241 gaagaaaaag tttggatttg ggcaggtcaa aaccaacacg atgtgtgtgg ctattattgg
      301 ttaatgagtc aattaaaaaa ttttcaaagc agaatattta ttttatactt aaataatttg
      361 ccatttataa atgagaaagg tcaaatattt tatccgcagc atttatcaga aattcaaccc
      421 aaagaatttt taaaagcaaa aaaattagct cgtgaaatta ctttaagcga atttgagctt
      481 gaccctgatg aatggaaaaa acttagcaat gaaaatgcaa tggttagaat tttagaagga
      541 ggtaaaaaaa ttgtaagtaa agaagaaaat ttttatgaca atgaaatttt atctgcaata
      601 aataacgaac cacaaaagct caataaaatt ttactccata ttttaaataa aatgaaaata
      661 aaaacgggag acgttttttt agtttggaga ataaatgagt tggcaaagca aatgaaaata
      721 gaaattagtg gagattggaa taaaggatgg aaagaaatgg aaatgagttt gcttaaaact
      781 ccacaataaa ttacatgatt gataaatttg aattttaaaa tgatgtccga tacaaacaac
      841 aatgaatctg gtgtacaact gcaatttaaa aattggtttt tagattatgc atcttatgta
      901 attctagaac gtgctgtgcc tgctattgaa gatggattga aacctgtaca acgcagaatt
      961 cttcatgcaa tgaaagagat ggatgatggg cgttttaata aagtagccaa cattattggg
     1021 caggcaatgc aataccatcc tcatggagat gcaagcattg gtgatgcatt ggtaaatatg
     1081 ggccagaaag atttattaat agaaactcag ggaaattggg gagatataag aacaggagat
     1141 gatgctgctg caccacgtta tattgaagca agacttagca aacttgcttt agaaattgcc
     1201 tttaataata aaaccactaa ttggcaatta agttacgatg gcagaaaaaa tgagccaatt
     1261 gtattgccta tgaaatttcc attactgctt gctcaaggtg cagaaggcat tgcagtaggg
     1321 ttaagtacaa aaattttacc acacaatttt atcgaaatta ttgaagcctc tatcaaatat
     1381 ttaaaaggta agaagtttga actttatcca gattttcaaa ctggtggttt aatagatgta
     1441 agtaattata atgaaggtag aagaggtggc aaaataaaag tgagatgtat tattgaagaa
     1501 attgataaaa aaagcttagt aattaaagat gtgccatttg gtgttacggt agcacagctt
     1561 tgtgaaagta ttacaaaagc aaacgattcc ggaaaaatta aaataaaaaa acttactgag
     1621 tttacaggca aaaacgttga aattcaaatt gatttagccg ctggcatatc aagcgatatt
     1681 actatagatg ccttatatgc ttttactgat tgcgaagtaa gtattagccc caatgcatgc
     1741 attattgaaa ataacaaacc aaaatttgtt ggtgtgcagg aattattaaa aatttctgta
     1801 gatgaaacaa aaatgttatt acaaaaagag ctgcaaatta aattagcaga gcttgaagat
     1861 aaatggcatt atacttcttt agaaaaaata ttctttgaag aaaaaatata taaagaatta
     1921 gaacaaaaat atgcctcgtg ggagcttgtt ttacaagcaa ttgaaaatgc ctttcaacca
     1981 tttattaaaa agttaaaaag aaaaagtatt agtagagaag atgtgttgaa acttactgaa
     2041 aaacctgtaa gaagaattta taaattagat atagatgatt taatagaaca gataaaagct
     2101 attgatgctg aaattgtaca ggtaaataac catttagagc atcttacaga ttttacaatt
     2161 acctattttg aaaaccttca gaaaaaattt gcaaaaggta gagaaagaaa atcggaaatt
     2221 aaattgtttg acacaattaa agtgcaacat attaccattg ccaacacaaa attatatgtg
     2281 aatagggaag agggctttat aggaacaagt ttaaagaaag atgaattatt atttgaatgc
     2341 tccgacttag atgatattat tgtttttacc aaacgtggca taatgaaagt ggtaaaagta
     2401 ggagataaag tttttattgg aaaagacatt attcatgccg caatttttaa aaagaacgat
     2461 gaacgtacta cctacaacat gatttatgta gatgcaaaat cgggtattag ttatgcaaaa
     2521 agatttaatg taacaggtat taccagagat aaagaatatg acttaaccaa aggcagcgat
     2581 aaaagtaaag tacattactt ttcatcaaat gcaaatggtg aagcagaaat ggtaaaaata
     2641 attttaagcc ctaattgtac agctagaaat aaagaattag aattttattt tgaagaatta
     2701 gaaattaaag gccgaagcag ccagggaaat attgtaacta aatatgcagt aaaatctatc
     2761 aagtttaaag aagctggtag aagtacatta agtggaatta aactatggtt tgatgatatt
     2821 tatggtaggt taaatacaga agaaaaaggg cagtatttag gaatgtttga agacgataaa
     2881 ttacttctta tttacaagga cggatattat gaaattacag atacggaatt aacacaaaga
     2941 ttggatggag ataaaatttt actaatcgaa aaatttaacc ctcaaaaaat tattactgct
     3001 atttatttag ataatgataa gcaacaattt aatgttaaaa gattttatat agaaactaca
     3061 actttaaaat ctaaattttt atttattaaa gaaggagatg gaaattattt agaaacaatt
     3121 accacagcaa cagagccaat attgcaggtg caaacagggc gtggacaaca agtaagaaaa
     3181 gctaagttta aaatagacaa aatggttgaa gtaatgggct ggaaggcaat aggtgcaaag
     3241 cttatggact ttagtaaaac tgttgagatg gagtggatac aagtacctaa agtagaaaac
     3301 aatcaaacag aattatttta aataaaaaga gccaagtttt tcaactttgg ctctttatta
     3361 ataaaaccac aaaatataca atcatccaat tatttatttg cgtataaata aaccattatt
     3421 tgtacgtcag aagcagcata tttatctgta ataggtaata atcttgaatc taagctaaag
     3481 aaagcttgag caaataattt tttatcagaa acaccacgta caataccagg aaacttaata
     3541 ggcactttaa taccctttat ggttaatgta cctgtaagtt ctacagcagt ttctgaagtg
     3601 taatttacat tttcaatctc aaaaattgct tccggatgtt ttgtaacatc aaaaaaatca
     3661 tcggctttta aatgcccttc taatttaacg ccagctttat ctgtaacttt taaattagcc
     3721 atatcaatta caaattgccc tttggttatt cttccatttt caacatttac atttccgctc
     3781 tttagcataa atgttccagg atggtatcct tcttttttac ttccaaccca atcaattttt
     3841 gagtgttccg tatttacttt ataatcatct gttacaaggg ttttaatacc aggtacaaaa
     3901 gaataaagtg cagtaaaaca ggcaatcgct aatgccgaaa ataaaacttt tttcatgttt
     3961 aattaattgt ttaggattca aatataatgg caaattgtgt gtatatacat gcaaataaat
     4021 tctttttcct tattttataa atttttaaca atttttagct agtaaaaatg gtgcaatttt
     4081 aattactgtt aaccttgttg tagttgaagt aaattaatgg tagtttttat ttttgtcaat
     4141 ttcttctcca agtctttctc ttatgtcaat ttttgcacca atgaaagcaa atattcctgt
     4201 accatattct cttgcataag ggtttgttat agagccagca agtaatgaat ttttaaagta
     4261 gggcgatatt ttttgtaatt cttcttttac ttcttctgct tctttaactc taattaggtt
     4321 tacatatttg ttggataagt caatccaatt aatatagtct gcattaaagg aaactgcttg
     4381 taatttttgc tttgtgtagt aatttattgc acctgcttgt ccatagttat cacaaagtat
     4441 tagtgtattt tgtttattgg gtaaggataa ataaatacta tccactttac tagctaattc
     4501 tttccagccc agcatatctg caaaatcttg tggtattaaa tggtcttttc catcttccca
     4561 acgtaataaa cccagtttct tataattttc tgaatgtttt actatgtatt cggggctttt
     4621 atttggaaaa gcaacattat aaataggaat aaagagtagt aaaggaataa taattgcaat
     4681 aggttgtaaa tattttttcc aaccaccttc tagaattttt gaaagataaa cagaaccaaa
     4741 tgctatgtaa attgggtaaa tgccaatggc ataataatcc tttgccttaa aataaacaaa
     4801 ggctgttaaa gtaaataaaa ttgaataaat atatactcta tacttagcaa aagggatata
     4861 aaacaaaaga gcataaaatg aggaaaagag tactaccagt gagcctataa aaaacagtaa
     4921 ttgatttttc aaaaaatcaa gtctatctac atttactaat tgagtttctg ttaattgtt
//
LOCUS       DAOQPK010000165         4902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_10725_length_4902_cov_4.195172, whole genome shotgun sequence.
ACCESSION   DAOQPK010000165 DAOQPK010000000
VERSION     DAOQPK010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10725_length_4902_cov_4.195172"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PLU36_08065"
     CDS             complement(<1..186)
                     /locus_tag="PLU36_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HMZ46741.1"
                     /translation="MGLNNIGKLFMPKNKIFYELFEGVAARVSDMGYKLKEMVNEPDA
                     DKRASILSVIEDLEHKND"
     gene            327..1637
                     /locus_tag="PLU36_08070"
     CDS             327..1637
                     /locus_tag="PLU36_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HMZ46742.1"
                     /translation="MRKHLIKSLVFVIGLLYCTISNAQFLMDMVDTTKETGKGLLNIY
                     KKFDHLKISCYIQPQFQIAQSKGIPTFEGPDFNEEVNNRFLLRRSRLRFDYVHFEENN
                     TPGVQIAFQFDANERGFTLRDVWGRVFENKYKLFSFTTGMFARPFGYETNLSSSVRES
                     PERGRMNQLLMKSERDLGAMISLDARKKISGLQYLKVDLGLFNGQGINAKGDFDNSKD
                     VVARVALKPYKISKNITVSMGASALMGGLLQNTKYVYYTSLENGIKKILVDSAIDNKG
                     KNAPRKYIGTDVQLKIKNRIGFTELRAEFIAGKHAVLENSNTTPTSLVTDKNGYYIRN
                     FNGAYFYFIQNIFSQKHQLVVKYDWFDPNSRTSKKDIGKVGSNFNSADIKYNTLGLGY
                     INHVSNNIKLTLYYAKVWNEKTQLTGYTKDVKDDVFTCRLQFWF"
     gene            1916..3016
                     /gene="atpB"
                     /locus_tag="PLU36_08075"
     CDS             1916..3016
                     /gene="atpB"
                     /locus_tag="PLU36_08075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HMZ46743.1"
                     /translation="MKSLLVLAFSLISLLFSNNTFAIGTADSTNSHHKAEKFDVAKTI
                     LDHIRDDYSWHLWGHTSIPLPVILYSDKGLEVFSSAKLMDEHHSPIIYQGNYSYKNIE
                     GKIKVVNADGTVNAELNSKVWDFSFTKNVASLFISVFILLAVFLTVGAAYKKRGVSSA
                     PKGLQSFMEPVVLFIRDEVAKPNIGHKYNTYLPFILTIFFLILINNLLGLVPFFPGGA
                     NLSGNIAFTMTLAVFVFIVVNIKGNKSYWGHIFWMPGMHWGMKLFLAPIELVGVFTKP
                     ISLTIRLFANMTAGHILVLSLICLIFIFENIFTSIVAVPFAAFIGLIECLVAFIQAFI
                     FAMLSSMYIGMAIEEHHHHGDNEPHYNESDEN"
     gene            3050..3274
                     /gene="atpE"
                     /locus_tag="PLU36_08080"
     CDS             3050..3274
                     /gene="atpE"
                     /locus_tag="PLU36_08080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HMZ46744.1"
                     /translation="MVLGAVVGSVAAIGAGIAAIGAGIGIGQIGKGAVEGISRQPEAA
                     NDIRSSMIIAAAFVEAVALFAVVVALLGNG"
     gene            3364..3876
                     /gene="atpF"
                     /locus_tag="PLU36_08085"
     CDS             3364..3876
                     /gene="atpF"
                     /locus_tag="PLU36_08085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HMZ46745.1"
                     /translation="MYLLEINPLVSPAIGLVFWNTVAFALLFFILRKFAWKPILNAIH
                     ERETGIENALSKAEKMKAEMKALANENEQLLQKAREERAVLIKEAKEQSDRMIADAKD
                     KAKAEYDRIVSDAQTAINQQKNAALTDVKNLVGTLVIEVSEKILRRELSNKAEQENYI
                     KQLAEEVKLN"
     gene            3912..4475
                     /gene="atpH"
                     /locus_tag="PLU36_08090"
     CDS             3912..4475
                     /gene="atpH"
                     /locus_tag="PLU36_08090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HMZ46746.1"
                     /translation="MQNPRLAHRYAKSLMDLSIEKKQLEEVCNDMKYLQALCSQSREF
                     TSLLKSPIIKADKKRNIINAVTEGKVSEITKLFNQLLVTKGRESSLAEIANAFIEEYN
                     TLKGIHKVVLTTAVELSKDVLNTIGNKIKTERGIDNIELEAKVNKDIIGGFILEFDNN
                     LVDASIVRDLRDVKKQFAKNIYIPEIR"
     gene            4512..>4902
                     /locus_tag="PLU36_08095"
     CDS             4512..>4902
                     /locus_tag="PLU36_08095"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788907.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HMZ46747.1"
                     /translation="MVNIKPDEISAILRQQLSNFNASADLEEVGTVLQVGDGIARVYG
                     LNNVRSGELVEFENGVRAIALNLEEDNVGVVLMGESGDIKEGAKVRRTGQIASIKVGE
                     GVVGRVINTLGEPIDGKGPIHGELYEMP"
BASE COUNT         1773 a          653 c          888 g         1588 t
ORIGIN      
        1 atcatttttg tgctctaaat cttcaatcac agataatata ctagcccttt tatcggcatc
       61 tggctcatta accatttctt taagtttata acccatatcg ctaactcttg ctgcaacacc
      121 ttcaaacaac tcataaaaaa ttttgttttt aggcataaac aatttgccaa tgttatttaa
      181 tcccattatt tataatttta ttatttgcaa agttgattat tttacttaat gattaatgca
      241 acctgtaaat tggtaataat tccttaacat tggaaataaa tttgtatttt tctttacaca
      301 ttatttttac atttctaaac aaaaacatga gaaagcacct tatcaaaagc cttgtatttg
      361 ttattggttt attgtattgc actatttcaa atgctcagtt tttgatggat atggtagata
      421 caaccaaaga aacaggtaaa ggccttttaa atatatataa gaagtttgac catttaaaaa
      481 ttagttgcta tatacaacct caatttcaaa ttgctcaatc taaagggata cctacatttg
      541 aaggaccaga ttttaatgaa gaagttaata atagattttt attaagaaga agcaggctaa
      601 gatttgatta tgtgcacttt gaagaaaaca atacaccagg tgtgcaaatt gcttttcaat
      661 ttgatgcaaa tgaaagaggg tttactttaa gagatgtgtg gggcagagtt tttgaaaata
      721 agtataaact tttttcattt actacaggca tgtttgcaag accatttggt tacgaaacca
      781 atttaagcag tagtgttaga gagtctccag agcgtggaag aatgaaccag cttttaatga
      841 aaagcgaaag agatttaggt gcaatgatta gtttggatgc aaggaaaaaa attagtgggc
      901 tacaatattt aaaagtggat ttaggcctat ttaatggcca gggaattaat gctaaaggag
      961 attttgataa tagtaaagat gtagtggcaa gagttgcttt aaagccatat aaaatttcaa
     1021 aaaacataac tgtatcaatg ggagcttctg ctttaatggg tgggttatta caaaacacaa
     1081 agtatgtcta ttacacatca ttagagaacg gaataaaaaa aatattggtt gattcagcaa
     1141 tagataataa aggaaaaaat gctccaagaa aatatattgg aacagatgta cagcttaaaa
     1201 ttaaaaacag aattggcttt accgaattaa gagcagagtt tattgcagga aaacatgctg
     1261 ttttagaaaa tagcaacacc acaccaacca gcttagtaac tgataaaaat gggtattata
     1321 tcagaaattt taatggagca tatttttatt tcattcaaaa tattttctca caaaaacacc
     1381 aattggtagt taagtatgat tggtttgacc ccaactcaag aacctcgaaa aaagatattg
     1441 gtaaagttgg ctctaacttt aacagtgcag atataaaata taatacatta ggattgggat
     1501 atataaacca tgtttcaaat aatataaaac taactctgta ttatgccaaa gtgtggaatg
     1561 aaaaaacaca gctaacaggt tatacaaaag atgtaaaaga tgatgtgttt acctgcagat
     1621 tacaattttg gttttaattt tttgtgcatt tttttaatgc taacccctaa ttttttttaa
     1681 aaatttttca ttttatatgc tatgtgtttt aaaaaccagg tataaatcta taagtagtat
     1741 aaaaaaccaa gtattcttat taaagttgaa actattttag acaatgaagt acttattaat
     1801 gtataaacaa atttatcttc aataattttt aaaaaaccaa ttcataaacc aatctaatat
     1861 caaacatacc cctatctttg ccgcaaattt caagaaggat atggtgtcaa gaagcatgaa
     1921 gtctttgctg gtattggctt ttagccttat aagcctttta ttttcaaata atacatttgc
     1981 aatcggaact gcagattcta caaatagcca tcataaagca gaaaagtttg atgtggctaa
     2041 aactatttta gaccatatta gagacgatta tagctggcat ttatgggggc acacatctat
     2101 tcctttacct gtaattctat attcagacaa gggtttagag gttttctctt ctgcaaagtt
     2161 gatggatgag caccatagcc ctatcattta ccaaggaaat tacagttaca aaaatattga
     2221 aggtaaaatt aaagttgtaa atgccgatgg tacagttaat gcagaattga acagtaaagt
     2281 gtgggatttt tcatttacaa aaaatgtagc atcgttattt atttccgtat ttattttatt
     2341 agcagttttc ttaactgttg gtgcagccta taaaaaaaga ggtgtctcat cggcacctaa
     2401 aggcttacaa tcatttatgg agccagttgt attgtttatt agagatgaag tagctaaacc
     2461 aaatattggc cataaataca atacttattt accatttata ttaactattt tctttttaat
     2521 attaataaat aacttgttag gattagttcc tttctttccg ggtggtgcaa accttagtgg
     2581 taatattgca tttacaatga cgttggcagt atttgtattt attgtggtaa atattaaagg
     2641 taataaaagc tattgggggc atattttttg gatgccagga atgcattggg gaatgaaact
     2701 atttttagca cctattgaat tggttggtgt atttacaaag cctatttcgc taactatacg
     2761 attatttgcc aacatgactg ctgggcatat tttagtactt agcttaattt gcttaatttt
     2821 tattttcgaa aatattttta catctatagt agcggttcct tttgcagcat ttattggttt
     2881 aattgaatgt ttggtagcat ttatccaagc tttcattttt gccatgttaa gttcaatgta
     2941 tattggaatg gctatagaag aacatcatca tcatggagat aatgagccac attataatga
     3001 gagtgatgaa aattaaattt ttatttttta acaattaaaa caaacaaaca tggtattagg
     3061 tgcagttgta ggtagcgtag cagccattgg tgcaggtatt gcagcaatag gtgcaggtat
     3121 tggtattggc caaattggta aaggtgccgt agaaggtatt tctcgccaac ctgaagcagc
     3181 taacgacatc cgttcaagca tgatcattgc agcagctttc gtagaagcgg tagcattatt
     3241 tgctgtggtg gtagcccttt taggtaacgg gtaatctact tttaaaagaa atcaagctca
     3301 atgcaaaggg caaagagctt gatttttaaa aacgatttct tatttagaat ttaattatac
     3361 aatatgtatt tgttagaaat taacccttta gtgagtccgg caattggctt agtattttgg
     3421 aatactgtgg cttttgcatt attgtttttt attcttcgca agtttgcttg gaaaccaatt
     3481 ttaaatgcca ttcacgaacg tgaaacaggc attgaaaatg cattaagcaa agcagaaaaa
     3541 atgaaagccg aaatgaaggc tttagctaat gaaaatgagc aattgttaca aaaagccaga
     3601 gaagaaagag ctgttttaat taaagaagca aaagaacaat ctgatagaat gattgccgat
     3661 gctaaagata aagcaaaagc agagtatgat agaatagttt ctgatgctca aactgccatt
     3721 aaccaacaaa aaaatgcagc tttaaccgat gtaaaaaacc ttgttggcac attggtaata
     3781 gaggttagcg aaaaaatatt aagaagagaa ttaagcaata aagcagagca agaaaattat
     3841 attaaacaat tagccgagga ggtaaaatta aattaagcag taactgctaa ttcttcatta
     3901 ttaattgagt tatgcaaaat ccacgtttag cacataggta cgcaaaaagt ttaatggatt
     3961 tatccattga aaaaaaacag ttagaagagg tttgtaatga tatgaaatat ttgcaagcac
     4021 tttgctctca aagtcgtgag tttaccagtt tattaaaaag cccaattata aaagcagata
     4081 aaaaaagaaa tattattaat gcagttaccg aaggaaaggt gagcgaaatt actaaattgt
     4141 ttaaccagct tttagttact aaaggaagag agagcagcct tgccgaaatt gcaaatgctt
     4201 ttattgaaga atacaacaca ttaaaaggta ttcataaagt ggtacttacc acagcagttg
     4261 aattaagtaa agatgtttta aatacaattg gcaataaaat aaaaacagaa agaggaattg
     4321 ataatattga attagaagca aaagtaaata aagacattat tggaggattt attttagaat
     4381 ttgataataa ccttgttgat gcaagcatag taagagacct aagagacgtg aagaagcagt
     4441 ttgcaaaaaa tatttatatt cctgaaatta gataagcaca aattaatttt aaatttttat
     4501 aattctatta tatggtaaac attaaacccg atgaaatatc tgccatttta aggcagcaac
     4561 ttagcaactt taatgctagt gcagatttgg aagaagtagg cacagtatta caagttggtg
     4621 atggtattgc acgtgtatat ggtttaaata atgtacgcag tggtgagttg gttgaatttg
     4681 aaaatggtgt aagagcaatt gccttaaact tagaagaaga taatgtaggt gtggttttga
     4741 tgggagaaag tggagacatt aaagaaggtg ccaaagttcg tagaactggg caaattgctt
     4801 ctattaaagt aggagaaggt gtagtaggcc gtgtaatcaa tactttagga gaaccaatag
     4861 atggcaaggg cccaattcat ggagaattat atgaaatgcc tt
//
LOCUS       DAOQPK010000166         4873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_10823_length_4873_cov_4.352013, whole genome shotgun sequence.
ACCESSION   DAOQPK010000166 DAOQPK010000000
VERSION     DAOQPK010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10823_length_4873_cov_4.352013"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PLU36_08100"
     CDS             complement(<1..353)
                     /locus_tag="PLU36_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HMZ46748.1"
                     /translation="MIEFFQHFMSEIEIPLKNPVLVFSLILLIILLSPLLLKKINIPG
                     IIGLIIAGVVIGPHGFNILAKNSAIDLFSTIGLLYIMFIAGLELDLNEFKANKNKSLL
                     FGFFTFIIPLSIGFPV"
     gene            710..1210
                     /locus_tag="PLU36_08105"
     CDS             710..1210
                     /locus_tag="PLU36_08105"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HMZ46749.1"
                     /translation="MQELYYTYYESPIGLIRIGGTDNFIAELVFIDNKDQIKHGEPGL
                     SDLMHVCTEQLIEFFAGKRKQFNVPIHQEGTAFQGNVWSELIHIPFGSTISYLDLAKK
                     IGDAKATRAVAAANGKNNIAIIVPCHRVIGSDKSLTGYAGGMWRKKWLLQHEFRIKNG
                     IQVLFN"
     gene            1326..2534
                     /locus_tag="PLU36_08110"
     CDS             1326..2534
                     /locus_tag="PLU36_08110"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ46750.1"
                     /translation="MKKTFLPILLLSALFVNQSSAQSNKAYAITAETKGNYNWTAIRE
                     IDLNNGNVIRTLYSNSNKPKVSYEFINNDWQANRVNIQSMSPTVNGVAAAAFDEKNNR
                     LYFTPMWGTDLRYVDLNTTEMNVVVNGDARFSTGKRVDESNVITRMVIAADGNGYALT
                     NDANQLIQFTTDKNPTITNLGALVDSKKNNGISVHTQCTSWGGDLIADVYGNLYLITF
                     RNHIFKINPTSKIAEHIGTIKGLPENFTTNGAAIDANGEVFISSAIQTNNYYKVNLST
                     LNASVIVKEGNETVYNASDLANSNLAYRNTRNNIVPTTVKNNGIAIYPNPVVNKTFAI
                     QFEKLPAGNYGVEITDVNGKRILNKTINIIGLQNEKINLPRASASGLYFVKITNTAGK
                     AIFTDKIVVE"
     gene            complement(2635..4026)
                     /locus_tag="PLU36_08115"
     CDS             complement(2635..4026)
                     /locus_tag="PLU36_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HMZ46751.1"
                     /translation="MKEPRSFKIFIVEDDLWYSSMLSYTLSLNPDYQIKKFESSKTFI
                     AALHEKPDVVSLDYSLPDATGEDVLDKINELSPETRVIVISGQEDVKVAINLLKKGAY
                     DYIVKDEDTKDRLWNSIQHLRENADLKKEIEVLKEEVAKKYDFAKTIIGNSIAIKRVF
                     NLMEKAVKNNITVSLTGETGTGKEVAAKCIHYNSDRRNKSFVAINVAAIPKELLESEL
                     FGHEKGAFTGAAVRRIGKFEEANKGTLFLDEIGEMDMNMQSKLLRALQERELSRVGSN
                     EIIKFDVRIIVATHKNLLDEVKNGNFREDLYYRLLGLPINLPPLRERENDVLMIAKFY
                     MEQFCKENKIPSKVLTTEAKKKLLNHSFPGNIRELKSVIELATVMAEDTEILPEHLNI
                     VVQNVMENFAIKDATLKEFNVKLIQYYLDLYQCDVLKVAKKLDIGKSTIYRMIQNKEL
                     SVSKNVLDETIMN"
     gene            4216..>4873
                     /locus_tag="PLU36_08120"
     CDS             4216..>4873
                     /locus_tag="PLU36_08120"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HMZ46752.1"
                     /translation="MQEVKDIEQPIDELYERRTFIIDKGQEPLRIDKWLQIRTEGSTR
                     NKIQQAIDNGFVTVNNQKIKSNYKVKPLDEILVLSYLNPEYSELKIENIPLNIVYEDE
                     DVLVINKPSNMVVHPGVGNHTGTLLNGVAYHLLQQNPSLNEEELPRFGLVHRIDKNTT
                     GLIVLAKNGMAAAHLAKQFFNHTVKRNYVALVWGNLEANEGTINTHIARHKQYRKMFD
                     A"
BASE COUNT         1777 a          747 c          701 g         1648 t
ORIGIN      
        1 acaggaaagc caatggaaag tggaataata aatgtaaaaa aaccaaagag caaacttttg
       61 tttttgttgg ctttaaactc attcaaatct aattctaagc cagcaataaa cataatgtat
      121 aataagccaa tggtagaaaa taaatcaata gcagagtttt tggctaaaat attaaatcca
      181 tgaggaccaa ttacaacacc tgctataatt aacccaatta tacctggaat gttaattttt
      241 tttaataaca atggagataa taaaataatt aacagtatta atgaaaaaac taaaactgga
      301 tttttcaatg gtatttctat ttctgacatg aagtgttgga aaaattcaat catagcttac
      361 attttataaa ttatagagaa tgaaaaaacg aatcatccac tcttacaata atacaaaaaa
      421 ttttaaagca accttaaata agtcattaac tattggttaa agaaaagttt catgaaacaa
      481 tcttcatgaa ctgtttccaa actttatatt tgttttatac tttcatctcg tatataatgt
      541 aaagcgtgtg gtttgattat aatttatact atcataaatt gggagcctaa caaactaaaa
      601 gtatagctta taaaactttt tgcaactctc cttattaaat gctattactt tacaacaatt
      661 attttattta cttcaattcg tataatttta ccagcagcaa aaaagcaata tgcaggaact
      721 gtattataca tactacgaaa gtcccattgg cctgattaga attggtggta ccgataactt
      781 tattgcagaa ttagttttta ttgacaacaa agatcaaatt aaacatggcg aacctggctt
      841 gagtgatttg atgcatgttt gtactgaaca attaattgaa ttttttgcag gaaaaagaaa
      901 acaatttaat gtccccattc atcaagaagg aacagctttt caaggaaatg tttggagtga
      961 attaattcat ataccttttg gtagtaccat tagttattta gatttagcta aaaaaattgg
     1021 agacgcaaaa gcaacaagag cagtagctgc agccaatggc aaaaacaata ttgctattat
     1081 tgtgccttgt cacagagtaa ttggtagcga taaaagccta acaggttatg ctggtggtat
     1141 gtggcgaaaa aaatggttat tacaacatga atttagaata aaaaacggca tacaggtttt
     1201 attcaactaa tagtactttt tcatttttct tctttactat ttgttaaata tttctattta
     1261 tcaaaaaatc attgaatatt atttttttct tcctaaattt aaccaacaat ttaaacttta
     1321 caactatgaa gaaaacattt ttacccatct tattactctc tgctttattt gtaaatcaat
     1381 ctagtgctca aagtaataaa gcatatgcta ttactgctga aactaaagga aattataatt
     1441 ggacagcaat aagagaaatt gatttaaata atggtaacgt tattagaact ttatacagta
     1501 attccaataa gcctaaagta agttatgagt ttattaataa cgattggcaa gcaaacagag
     1561 taaatataca aagcatgtct cctacggtaa atggcgttgc tgctgctgct tttgatgaaa
     1621 aaaataatcg tttatatttt acgccaatgt ggggtaccga tttacgttat gtagatttaa
     1681 acacaacaga aatgaacgtt gtagttaatg gcgatgcaag attttctaca ggcaaaagag
     1741 tggatgaaag caatgtaatt acaagaatgg taattgctgc agatggcaat ggttatgcac
     1801 tcacaaacga tgcaaaccaa ctaattcaat ttactactga taaaaatcct acaatcacta
     1861 atttaggagc attagtagat agcaaaaaaa ataatggtat tagtgttcat actcaatgca
     1921 caagttgggg tggcgattta atagcagatg tttatggaaa cttatactta attactttta
     1981 gaaaccatat ttttaaaatt aatccaactt caaaaattgc agaacatata ggtaccatta
     2041 aaggattacc agaaaacttt acaaccaatg gtgcagcaat agatgctaat ggagaggtgt
     2101 ttatttctag tgctatacaa acaaataatt actacaaagt aaacttatct accttaaatg
     2161 cttctgtaat agtaaaagaa ggcaacgaaa ctgtttacaa tgcatctgac ttagcaaata
     2221 gtaatttggc ttacagaaat acaagaaaca atattgtacc aacaacagta aaaaataatg
     2281 gcattgcaat ttatcctaac ccggttgtaa ataaaacttt tgcaattcag ttcgaaaaat
     2341 tgcctgcagg taattatgga gttgaaataa ctgatgtaaa cggtaaaaga attttaaata
     2401 aaacaattaa tattattggt ttacaaaacg aaaaaattaa cctaccaagg gcttcagcat
     2461 ctggtttata ttttgtaaaa atcaccaata ctgccggtaa agctatattt accgataaaa
     2521 ttgtagttga ataaattttc attaccctta tttaatttat taaacctatc aacgataaat
     2581 gccaccttta taagggtggc attttttcat tgaaccttgg attttaaaat actatcaatt
     2641 cataattgtt tcgtctaaaa catttttact aactgataat tctttgtttt gaatcatacg
     2701 gtaaatagta ctcttaccta tgtcaagctt cttagcaacc tttaatacat cgcattgata
     2761 aaggtccaaa taatattgta ttaatttaac attaaattct tttaaagtag catcttttat
     2821 ggcaaaattc tccattacat tttgtacaac aatattcaaa tgctcaggta atatttctgt
     2881 atcttctgcc attactgttg caagctcaat tactgatttt agctcacgaa tatttccagg
     2941 aaaactatga tttaataatt tcttttttgc ctctgtagtt aatactttag agggtatttt
     3001 gttttcttta caaaattgct ccatataaaa ttttgcaatc atcaacacat cattttctct
     3061 ttctcttaaa ggtggcaaat taattggcaa gcctaaaagc ctataataca aatcttctct
     3121 aaaattacca tttttaactt catctaataa gtttttatga gtagcaacaa ttattcttac
     3181 atcaaatttt ataatttcgt tactccctac tctactaagt tctctttctt gtaaagccct
     3241 taataattta gattgcatat tcatatccat ctcaccaatt tcatctaaaa ataaagttcc
     3301 tttattggct tcttcaaatt ttccaattct tcttacagca gcacctgtaa aagccccttt
     3361 ttcgtgtcca aaaagttcac tttctaaaag ctccttgggt atagctgcta cgttaatagc
     3421 aacaaaggat ttatttcttc tatctgaatt gtaatgaatg catttagctg caacttcttt
     3481 acctgtacct gtttcgccag ttaaggatac tgtaatattg tttttaacag ctttttccat
     3541 taaattaaaa actctcttaa tggcaatact gtttccaatt atagtttttg caaagtcata
     3601 ttttttggca acttcttctt ttaaaacttc aatttccttt ttaagatctg cattttctct
     3661 taaatgttga atgctattcc aaagcctgtc tttggtatct tcatctttta caatataatc
     3721 ataagcacct ttcttaagta agttaatagc aactttcaca tcttcctgac cagatattac
     3781 aattactcta gtttcggggc ttagctcatt aattttatct aaaacatctt ctcctgttgc
     3841 atctggtaag gaataatcta atgaaactac atcaggtttt tcgtgcaggg cagcaataaa
     3901 agttttagat gactcaaatt ttttaatctg ataatcaggg tttaagctca aagtgtacga
     3961 taacattgaa ctgtaccata aatcatcttc tacgatgaat attttgaagg aacgtggttc
     4021 tttcataaaa tgagatattg gatatacttc tataaacgca aaacttttct caaaattgta
     4081 taaatggaaa ttttatggga aatattttcc caaataaaga attttccatt tttgaaatag
     4141 atacaagaaa tgttacctta attaaaattt ttaactttaa tagggtacaa aaagtactgc
     4201 ttacatttgc aaaatatgca agaagtaaaa gatattgaac aaccaataga tgagttatac
     4261 gaacgtagaa cttttattat tgacaaaggg caagaacctc ttagaataga taaatggctg
     4321 caaataagaa cagaaggaag taccagaaac aaaattcagc aagcaataga taatgggttt
     4381 gtaactgtaa acaaccaaaa aataaaaagt aattataaag taaaaccttt ggatgaaata
     4441 ttggttttaa gctaccttaa tccagaatac tcggagctta aaattgaaaa tattccacta
     4501 aatattgtgt atgaagatga ggatgtactg gtgattaata aaccttccaa tatggttgtg
     4561 catcctggcg ttggcaacca tacaggtaca ttattaaatg gtgttgctta ccatttgtta
     4621 caacaaaacc cttctttaaa tgaagaagaa ctaccacgat ttggtttggt acatagaatt
     4681 gataaaaata caaccggctt aattgttttg gctaaaaacg gaatggcagc agcacattta
     4741 gctaaacagt tttttaacca tacggtaaaa agaaattatg ttgcattggt ttggggaaat
     4801 ttggaagcaa atgaaggaac aattaatacg catattgcaa ggcataaaca atatagaaaa
     4861 atgtttgatg ctt
//
LOCUS       DAOQPK010000167         4785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_11133_length_4785_cov_4.519239, whole genome shotgun sequence.
ACCESSION   DAOQPK010000167 DAOQPK010000000
VERSION     DAOQPK010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11133_length_4785_cov_4.519239"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PLU36_08125"
     CDS             complement(<1..224)
                     /locus_tag="PLU36_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HMZ46753.1"
                     /translation="MSMKTLTHASDTQLIHAFQERNANEALEVLINRYKDKIFSSILF
                     LVKDKYLAEDLFQDVFIKVIDTLRNNKYSE"
     gene            complement(513..968)
                     /locus_tag="PLU36_08130"
     CDS             complement(513..968)
                     /locus_tag="PLU36_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46754.1"
                     /translation="MLAKTRTIFKELVEIYTGKDKRLSQMASWSLSYAVSKNSTLLQP
                     HLKEIINQLNRKDVHDAVIRNALRILQDIEIPIKFHGTVMHTCFNFIETPSTPVAIKA
                     FSLTILYNLSKIYTEIKPELICLIETNIQHETAAFKSRGNKILKLLKNK"
     gene            complement(1051..2859)
                     /gene="uvrC"
                     /locus_tag="PLU36_08135"
     CDS             complement(1051..2859)
                     /gene="uvrC"
                     /locus_tag="PLU36_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HMZ46755.1"
                     /translation="MTTEDFLKIVSTIPTLPGIYKYFDIENELIYVGKAKNLKNRVRS
                     YFGKSVNNYKTHKLVNAITRIEFTIVDSEQDAFLLENALIKTYKPKYNILLKDDKTYP
                     YIVIKNEPFPRLFFTRKKINDGSEYLGPFTSLKKVKELLEFIKLYIPLRNCNLLLSPT
                     NIAKKKFKVCLEYHLGNCKGPCEDLQTVDNYNENLQQVRNILKGNITPVIQHLKVEME
                     EKATNLQFEKAAIIKRKIDDLETYQSKSIIVNHLLSNIDVFSIVSEHEVAYVNYLMIQ
                     NGTIVQTHNIQLSIPLEQTNQQVLSYAIPQIRSNFNSTANEIIVPFKVDIVHSSIKIT
                     IPKLGDKKKLLELSLKNVSYYKQELQQKKFLHLTTSSNIENNETLIELQQYLRLKDLP
                     THIECFDNSNFQGSYPVSAMVCFKNGVPSKNDYRKFNVKTVIGINDFATMQEVVYRRY
                     KRLIEEEKPLPQLIIIDGGKGQLSAALKSIKKLSIENKLTVIGLAKNEEQLFFNEDQQ
                     PITLPLNSKSLKLIRFIRDEVHRFGITFHRNQRSKGTFKNELELIPGIGKQTVTELLK
                     TFKSVNAIKSKTHTELSEVIGNKRAGILIDYFNSKN"
     gene            complement(2875..3450)
                     /locus_tag="PLU36_08140"
     CDS             complement(2875..3450)
                     /locus_tag="PLU36_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46756.1"
                     /translation="MKYILTFSTFFILLLSCKEQKKEQEVSQNNIDTLPYYNYTAAIN
                     TEADSLIKIKKQFLKIVTNPNNSNNASKISLSDFKNLVQLFTQQNISEPPLKQFYKEQ
                     IFTDLTTNSTIINYTTSKDSLPVKNISIMLDAKNANTLKRIDIKNIYSVPDTLITENY
                     AWIFGKEFYFVKYYEPTNSIAFTTKTNISFK"
     gene            3529..4608
                     /locus_tag="PLU36_08145"
     CDS             3529..4608
                     /locus_tag="PLU36_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46757.1"
                     /translation="MKKSFYWILFAICFIHNYVFANMASPYIGGTKQSSAISSKHIQI
                     HSENIHIAIDKNFKTAKFIVEYNILADTNGLQVPLLFIAEDFKENFKVWVDGNLVEIK
                     NVNELLTNNPTAFNQLFNGINNTNEISIYWDKNIGNVYQLRDLKYFETDIAKGNHKIK
                     VEYVASVQIDRSNWVKNYKLKYSLSPAKFWKSFGSLAITLELEGTQKVTTNLGLPIEK
                     NYSTINNWKFTTLPADFIEISFTPTISSYAETLISMGPFGISMVFTILLFAINLYLIF
                     WYRRSNINKKYSPPVIIGSLLIPLISIIIYMYSYSFIDNAIGEEAGKYHGYFILVIII
                     YPILFLVYLLFTWLIDKLYKRKLLK"
     gene            complement(4600..>4785)
                     /locus_tag="PLU36_08150"
     CDS             complement(4600..>4785)
                     /locus_tag="PLU36_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46758.1"
                     /translation="FRKWNINTGLDLNFIYKVGNYSFFLSPQLRYQHLPTYTDEYPIK
                     EYRLDNGLRFGFTKKLF"
BASE COUNT         1591 a          597 c          661 g         1936 t
ORIGIN      
        1 tcttctgaat acttattgtt tcttaatgta tctattactt taataaacac atcttgaaat
       61 aagtcttcgg ctaaatactt gtcttttact aaaaataaaa tagaagagaa aattttgtcc
      121 ttgtagcgat taattaatac ttcaagggct tcattagcat ttctttcttg aaacgcatga
      181 attagttgcg tatctgacgc atgagttaaa gttttcatag acatctaggt ttttgatttg
      241 atctaaaata ttaggataat ggataacaaa aatcaattac attttttatt tttttggtgt
      301 aattaatttt atgatgtgaa gataggaatg atttttatat ttccaaattt tggagcaatt
      361 tttttttaaa cttttttctg taagccttat aaataaaggt tttcaggggt atttactata
      421 aagtaaatag tatttttacg atagtttaaa gcagtagtct aaaattgaga taataattct
      481 ataattggta atacttggtt gtaagtacaa tcctacttgt tttttaagag ctttaaaatt
      541 ttatttcctc tacttttaaa agcagcagtt tcatgctgta tattggtttc tattaagcaa
      601 attaactcag gttttatctc ggtatatatt ttacttaaat tgtacaatat ggttaatgaa
      661 aaagctttta tagctacagg ggttgatggt gtttcaataa aattaaaaca agtatgcatt
      721 actgtaccat gaaattttat gggtatttca atatcttgta aaattcttaa agcatttctt
      781 ataactgcat cgtgtacatc ttttctattt aattggttta ttatttcttt taaatgaggt
      841 tgtagtagag tagagttttt gcttactgca taacttaaac tccatgatgc catttgagac
      901 aatcgtttat ccttacctgt ataaatttcc accaattctt taaaaatggt tcttgttttg
      961 gcaagcataa gttgcaatag cttctatatt aatttttgaa taactatttt gtagcgattt
     1021 ttttatatcc aatgtataag aattaaggaa ttaatttttt gaattgaaat agtctattaa
     1081 aataccagcc cttttatttc caataacttc tgaaagctct gtatgggttt tgctttttat
     1141 agcattaacc gatttaaatg tttttaataa ttcggttact gtttgtttac caattccagg
     1201 tataagttct aactcgtttt taaatgttcc tttagaacgt tggttacgat gaaaggtaat
     1261 tccaaatctg tgaacttcat ctctaataaa cctaataagc tttaaacttt tactgtttaa
     1321 tggtagtgtt ataggttgtt ggtcttcatt aaaaaataat tgttcttcgt ttttagctaa
     1381 accaataaca gttaatttat tttcaataga tagttttttt atactcttca aggcagcact
     1441 taactgccct ttgccaccat ctataattat tagttgtggt aaaggttttt cttcttctat
     1501 taatcttttg taacgcctat aaacaacttc ttgcatagtt gcaaaatcat taataccaat
     1561 tactgtttta acattaaatt ttctataatc gtttttactg ggtacaccat ttttaaaaca
     1621 aaccattgca ctaacagggt aactgccttg aaaattagag ttatcaaaac attcaatatg
     1681 tgttggtaaa tcttttaatc ttaaatattg ctgtagctct atcaaagttt cattattttc
     1741 tatattgcta ctggttgtta aatgcagaaa cttcttttgt tgtaattctt gcttataata
     1801 agaaacattc tttaaagata gttctagtag ttttttctta tctcctaatt ttggaattgt
     1861 tatcttaatg gatgaatgaa caatgtccac cttaaaaggc acaattattt cattggcagt
     1921 gctgttaaaa ttgcttctta tttgaggtat tgcataactt aaaacttgct gattagtttg
     1981 ctctaaagga atacttaatt gtatattatg cgtttgcaca attgttccat tttgaatcat
     2041 taagtaattt acataagcaa cttcatgttc actaacaata gaaaatacat ctatattgct
     2101 tagtaaatgg ttaacgatta tagatttaga ttggtaagtt tctaagtcat ctatttttcg
     2161 tttaattata gctgcttttt caaattgcaa attggtagct ttctcctcca tttctacttt
     2221 taaatgctgt ataacgggtg taatatttcc ctttaaaata tttctaacct gttgtaaatt
     2281 ttcattgtaa ttatctactg tttgcaaatc ctcacatgga cctttacagt tgcctaaatg
     2341 atactctaaa catactttaa atttcttttt tgcaatatta gttgggctta ataataaatt
     2401 gcagttgcgt aaaggaatgt acagtttgat aaattctaat aattctttta ctttctttaa
     2461 tgaagtaaaa gggcctaaat attctgagcc atcattaatt ttttttcttg taaaaaataa
     2521 tctaggaaat ggttcatttt ttataacaat gtaagggtag gttttatcgt cttttaataa
     2581 aatattatac ttaggcttat acgtttttat taatgcattt tctagcaaaa aggcatcttg
     2641 ctcactatct acaattgtaa actcaatacg tgttatagcg tttacaagtt tatgggtttt
     2701 ataattattt accgatttgc caaaataact tctaactcta ttttttaaat ttttggcttt
     2761 accaacataa ataagttcat tttctatatc aaagtattta taaattccag gaagggtagg
     2821 aattgtagat acaattttta gaaaatcttc agtagtcata atagctttta ataattattt
     2881 aaagctaatg ttagttttgg ttgtaaaagc aatagaattg gtaggctcgt agtattttac
     2941 aaaataaaat tctttgccaa aaatccatgc atagttttca gtaattaaag tgtcgggtac
     3001 tgaataaata tttttaatgt caattctttt tagtgtgttg gcatttttag catctaacat
     3061 tatacttata tttttaacag gtaaactgtc ttttgaagtt gtataattta taatggtact
     3121 attagtagtt aaatctgtaa aaatttgctc tttataaaac tgttttaacg gaggttctga
     3181 aatattttgt tgggtaaata attgaactag atttttaaaa tctgaaaggg aaattttaga
     3241 tgcattatta gaattattag gattagttac tatttttaaa aattgttttt taatttttat
     3301 caaactatcc gcttctgtat taatagctgc tgtatagtta taataaggca atgtatctat
     3361 attattttga gatacttctt gctctttttt ctgttcttta caacttaata ataaaataaa
     3421 aaatgtacta aaggttaaaa tatatttcat aaataaaatt agttgatatt gttttaatgg
     3481 cttatttttc ctgtaaagaa atgattaatt gataaaaata gtttaaaaat gaagaaatct
     3541 ttttattgga tattatttgc aatctgtttt attcacaatt atgtttttgc caatatggct
     3601 tcgccatata taggaggcac aaagcaaagt tctgctattt ctagtaaaca cattcaaatt
     3661 cattcagaaa acattcatat tgctattgat aaaaatttta aaacagcaaa attcattgtt
     3721 gagtataata ttttagcaga taccaatggg ctacaagttc cattattgtt tattgcagaa
     3781 gattttaagg aaaattttaa agtatgggtt gatggcaatt tggttgaaat taaaaatgtt
     3841 aatgaacttc ttactaataa ccctacagca ttcaatcaat tgtttaatgg aattaataac
     3901 accaatgaaa taagtattta ttgggataaa aatattggca atgtttatca actaagagat
     3961 ttaaaatatt ttgaaacaga tattgccaaa ggtaatcaca aaataaaggt tgaatacgtt
     4021 gcatcagtac aaatagatag atctaattgg gtaaagaatt ataaacttaa gtattcattg
     4081 tctcctgcaa agttttggaa atcatttggc agtttagcaa ttactttaga gttagaagga
     4141 acgcaaaaag ttactaccaa tttaggatta cctatagaaa aaaactattc tacaattaac
     4201 aactggaagt ttactacttt gcctgccgac tttattgaaa tatcttttac accaactatt
     4261 agcagctatg ctgaaacatt aataagcatg ggcccgtttg gtataagtat ggtttttact
     4321 atactattat ttgctattaa tttgtattta attttttggt atagaagaag taatattaat
     4381 aaaaaatact ctcctcctgt aattatagga agtttattaa tacccttaat ttctataatt
     4441 atttatatgt atagttattc ttttattgat aatgcaatag gtgaggaagc agggaaatat
     4501 catggatatt tcatattagt aattattatt tatcctattt tatttttagt ttatttatta
     4561 tttacctggc taattgacaa actttataaa agaaaattat taaaataatt ttttagtaaa
     4621 tccaaatctt aaaccattgt ctagcctgta ttcttttatt ggatattcat cggtatatgt
     4681 aggtaaatgt tgatagcgta attgtgggct taaaaagaaa ctataattac caactttgta
     4741 aataaagttt aaatctaatc cagtattaat attccatttt ctaaa
//
LOCUS       DAOQPK010000168         4782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_11141_length_4782_cov_4.250688, whole genome shotgun sequence.
ACCESSION   DAOQPK010000168 DAOQPK010000000
VERSION     DAOQPK010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11141_length_4782_cov_4.250688"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="PLU36_08155"
     CDS             complement(<1..1430)
                     /locus_tag="PLU36_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018631009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HMZ46759.1"
                     /translation="MSLRFSAINANLSKSDINYIGPTKITPLFGENVFTLKVAREYLS
                     DEAYKSLSTSINTGKKLDRSVANQIAIGLRAWSENKGVTHFTHWFQPLTGTTAEKHDS
                     FFTLKSDGTAIEEFDGAALVQQEPDASSFPNGGLRATFEARGYTAWDPSSPAFIMEMC
                     NGKTLCIPTIFISYTGESLDYKAPLLKALEALNKSAVDVCNYFDKNITKVSATLGWEQ
                     EYFVIDEGLANARPDIMQCGRTVFGASPAKGQQLEDHYFGSIPDRIYTYMRDFEAEAY
                     KLGIPLRTRHNEVAPGQFECAPIFEEVSVAVDHNTLLMDLMERVASRHQLKVLFHEKP
                     FDGINGSGKHNNWSMSTDTGVNLLAPGKTPKTNLMFLTFFVNSIKAVHDYADLLRAAI
                     ASAGNDHRLGANEAPPAIISVFVGQYLAKVLEEVETRVGDKFDEQDEAILKLDIHKSI
                     PELLLDNTDRNRTSPFAFTGNKFEFRA"
     gene            complement(1539..2801)
                     /locus_tag="PLU36_08160"
     CDS             complement(1539..2801)
                     /locus_tag="PLU36_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46760.1"
                     /translation="MQIILFLLYLILFCFALTRIKFFTNSNLEKPILISLFLIKVLAG
                     LAYAWFYTLPAYHINSDSFRFYAYSLEETNILLTQPLHFFKDIFTYGYQTTGNVFVGD
                     NSYWNDLKSNIIIKLLAVCNVFSFKNYYINIIFFNFLFFFGLIAFYRLMQNVFNVNKV
                     ILILPIFFIPSFLFWCSGIHKDGLLFSAIGMVFFYFNVCLLKGYSFKKVALVLSLLFI
                     IFFLRSYVALLLLLAMFAWWVAQQSKKHLLSFILVYVVAIILFFISPFIHSKLNFPNY
                     ISVKQHEFSILDGGSSIIAKSIDPSFAGFLHYLPNALDMAFMQPHIFNFKNLSYIPAI
                     IENLGMIIILIILLVKRKKKIVMPPVVYACLFFGITMLLIAGYTVTFSGAIVRYRSLV
                     LPILVTLVFANINFESIFKNKNLFKLKK"
     gene            complement(2865..4124)
                     /locus_tag="PLU36_08165"
     CDS             complement(2865..4124)
                     /locus_tag="PLU36_08165"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HMZ46761.1"
                     /translation="MKTRRAFLKNTGLAMFGIGLGGVPGFVVKAAESFKQQNLYKKNK
                     ILICIFQRGAMDGLMAVTPFTDQFLKAARPNLFIEAGNNNSLIDLDGRFGLHPSLNAF
                     KKLFDEKRLAIVHGIGSPNNTRSHFDAQDYMESGTPFSKSTSSGWLNRAVGLMGHDAT
                     PFRAVSLTATLPRSFYGNNEAIAMSSIKSFALQSNNVSSTSINVGKSLEEMYDETSTN
                     IINATAKESFEAIKILKNAKIENYQPENGAVYPNSDLGKSLNQIAQLIKMNVGLEVAF
                     AEHGGWDTHFNQGKETGIFARNAEDLSNSIFALWTDLEKFQDDVVIMTMTEFGRTVKQ
                     NGTGGTDHGRASCNFIIGNKISGGRVFGNIQPLAVENLEDGRDLAVTTDFRNVFSEVA
                     TKHLNIINHQALFPSFEEKYLNITKKV"
     gene            complement(4136..>4782)
                     /locus_tag="PLU36_08170"
     CDS             complement(4136..>4782)
                     /locus_tag="PLU36_08170"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HMZ46762.1"
                     /translation="IKQVILTMVSSPEFWNKDALREKVKSPFELAISSIRVLHAEVKR
                     PFTVYNWVSNMGQKLYAYQAPTGFPDNSNYWINTGTLINRMNFGLLIATNKLPGIKYN
                     VSALNNDQEPESAENALAVYAKILLPQRNIDATIKRLKPLLNEQNLESKLNKSKVQNV
                     ANTSNQSTMDDEEDIFQNDILSTYEKENRKEEGHNKTLMQIIGIILGSPEFQRR"
BASE COUNT         1645 a          831 c          627 g         1679 t
ORIGIN      
        1 gctctaaact caaatttatt tcctgtaaat gcaaaaggtg atgttctatt tctatcggta
       61 ttatctaata ataattcagg aatactttta tgtatatcta atttcaaaat agcttcatct
      121 tgttcatcaa acttatctcc tacccttgtt tctacttctt ctaaaacttt tgctaaatat
      181 tgccccacaa ataccgaaat aattgctggt ggtgcttcat tagcacccaa cctatggtcg
      241 ttacctgcac ttgcaatggc agctcttaat aaatcggcat aatcatgcac tgctttaatg
      301 ctatttacaa aaaatgtaag aaacattaaa tttgttttag gagttttgcc aggtgctaat
      361 aagtttacac ctgtatctgt actcatactc cagttattat gttttccact accattaata
      421 ccatcaaatg gtttttcatg aaaaagaact tttaattggt gcctgctagc aacacgttcc
      481 attaaatcca taagcagtgt attgtgatct acagcaacac tcacttcttc aaatatagga
      541 gcacactcaa actggcctgg agccacttca ttatgccttg ttcttaatgg aatacctaat
      601 ttataagctt cagcctcaaa gtctctcata taggtataaa ttctatcggg aatagagcca
      661 aaataatgat cttctaattg ttgtcctttt gcaggtgatg caccaaaaac tgttcttccg
      721 cattgcataa tgtctggtct ggcatttgcc aatccttcat ctattacaaa atattcttgc
      781 tcccagccaa gtgtagcaga aaccttcgta atatttttat caaaataatt acaaacatct
      841 actgcacttt tattaagtgc ttctaatgct tttaataaag gtgctttata atccaaagat
      901 tcgcctgtat aggaaataaa aattgtagga atacacaagg ttttaccatt acacatttcc
      961 ataataaatg caggagaaga aggatcccat gcagtataac ctcttgcctc aaaagtggct
     1021 cttaatccac catttggaaa ggaggaagca tctggctctt gttgcaccaa tgcagcacca
     1081 tcaaattctt caatagctgt accatcgctt tttaaggtaa aaaatgaatc atgtttttct
     1141 gcagttgtac ctgttaatgg ttgaaaccaa tgcgtaaaat gggttactcc tttattttca
     1201 gaccatgctc ttaagccaat tgcaatttga ttagctacag aacgatccag ttttttacca
     1261 gtatttatac ttgtacttaa gcttttataa gcttcatcac tcaaatattc tctggctact
     1321 tttagtgtaa atacattttc tccgaacaaa ggagtaattt ttgttgggcc aatataattt
     1381 atatctgatt ttgaaagatt ggcattaatt gccgaaaacc gtaatgacat atatttttta
     1441 tttattcatg caaatatgag gtcattattt caaaaaacag aaattttttg aataattttt
     1501 caaaaaataa tacattatcc ttaaaatagt ttacatattt atttttttaa tttaaataaa
     1561 ttcttattct taaaaatact ttcaaaatta atattggcaa aaactaatgt aactaaaatt
     1621 ggtaaaacca agcttctgta acgtactatt gctccagaaa atgtaactgt atatcctgca
     1681 attaataaca tagtaatacc aaaaaataaa caagcataaa caacaggtgg cataacaatt
     1741 tttttctttc ttttaaccag caggataatt aatattatta tcattcctag gttttcaatt
     1801 atagcaggta tataacttaa atttttaaaa ttaaaaatat gaggttgcat aaaagccata
     1861 tctaaagcat taggcaaata atgtaaaaaa cctgcaaaac taggatcaat gctttttgca
     1921 ataatggaac tacctccatc taagattgaa aattcatgtt gttttacaga aatataatta
     1981 ggaaaattta gttttgaatg tatgaatggt gatataaaga aaagaatgat tgcaacaaca
     2041 taaaccaaga taaagcttaa taaatgtttt ttagattgtt gggctaccca ccatgcaaac
     2101 attgccaaca acaacagcaa agcaacataa cttcttaaaa agaaaataat aaaaagtaaa
     2161 gacaatacaa gtgctacttt tttaaaacta tatcctttta ataaacatac attaaaataa
     2221 aaaaatacca taccaattgc agaaaacaat aatccatctt tatgaatacc actgcaccaa
     2281 aacaaaaagg atggtataaa aaaaattgga agaatgagta ttactttatt aacgttaaaa
     2341 acattttgca ttaacctata aaaagcaatc aatccaaaaa agaaaaggaa attaaaaaat
     2401 ataatattga tataataatt cttaaaacta aaaacattgc atactgctaa taatttaata
     2461 ataatattac tctttaaatc attccaataa gaattatcgc ctacaaaaac attaccagtt
     2521 gtttggtaac cataagtaaa aatatcttta aaaaaatgta atggttgggt aagtaaaata
     2581 tttgtttctt ctaaactgta agcataaaaa cgaaaactat cgctattgat gtggtatgca
     2641 ggaagtgtgt aaaaccaagc ataagcaagc ccagcaagaa ctttaattaa gaataaacta
     2701 attaatatgg gtttctctaa attagaatta gtgaaaaact ttattctggt tagtgcaaaa
     2761 cagaacaata ttagatacaa taaaaaaagt ataatttgca agtataaaat ttggagcaat
     2821 ttaagaaaaa ctgcattata aattttttat actattattt aagtttacac tttttttgta
     2881 atgtttagat acttttcttc aaaacttggg aataaagctt ggtgattaat tatgtttaaa
     2941 tgtttagttg caacctcgct aaaaacattt ctaaaatctg ttgttactgc aagatctctt
     3001 ccatcttcta aattctctac tgctaaaggc tgtatattac caaatactct tcctccagat
     3061 attttattgc caataataaa attacaacta gctcttccat gatctgtacc tcctgtacca
     3121 ttttgcttta ctgttcttcc aaattcagtc atagtcataa taactacatc atcttgaaat
     3181 ttttccaaat cagtccataa tgcaaatatg ctattgctaa ggtcttctgc gtttctagca
     3241 aaaatgcccg tttcttttcc ttggttaaaa tgtgtatccc aaccaccatg ttcagcaaat
     3301 gcaacttcta atccaacatt cattttaatt aactgagcaa tttggtttaa gcttttacct
     3361 aaatcagaat ttggatatac agccccattt tctggttgat aattttctat tttagcattt
     3421 tttaaaattt taattgcttc aaaactttct tttgcggttg cattgataat atttgtagat
     3481 gtttcatcgt acatttcttc taagctttta cctacattga tagaggtaga tgacacattg
     3541 ttggattgta acgcaaaact tttaatgcta ctcattgcaa tggcttcatt atttccataa
     3601 aaacttctgg gtaaagttgc tgtaaggctt actgctctaa aaggtgttgc atcatgcccc
     3661 atcaaaccaa ctgctctgtt taaccagcca gaagaagttg acttagaaaa tggcgttccg
     3721 ctttccatat aatcttgtgc atcaaaatga cttcttgtat tatttgggct gccaatgcca
     3781 tgtacaatag ctaatctttt ttcatcaaat aatttcttaa atgcatttaa tgatgggtgt
     3841 aaaccaaacc ttccatctaa atcaattagt gaattgttat ttcctgcttc tataaataaa
     3901 ttaggccgtg cagcttttaa aaactgatct gtaaaaggtg taacagccat taaaccatcc
     3961 attgcaccac gttgaaaaat gcaaattaaa attttatttt ttttgtacaa attttgttgc
     4021 ttaaaacttt ctgctgcttt tacaacaaaa ccaggtacac cgccaagccc aattccaaac
     4081 atggctaacc cagtattttt taaaaatgct cttctcgttt tcataactat atcttttatc
     4141 ttctctgaaa ttcaggagaa cctaaaataa ttccaataat ttgcattaag gttttgttat
     4201 gaccctcctc tttgcgattt tctttttcgt aagtactaag aatatcattt tgaaaaatat
     4261 cttcttcatc atccattgta ctctggttgc tggtgtttgc aacattttgc acttttgatt
     4321 tgtttaactt gctctctaaa ttttgctcat ttaataaggg ttttagcctt tttatagtgg
     4381 catcaatatt tctttgaggt aacaaaattt ttgcataaac tgctaatgca ttttctgcac
     4441 tttcaggttc ttggtcatta tttaaagcac taacattata tttaatgcct ggcaatttat
     4501 tggtagcaat taataaccca aagttcattc tattaattaa agttcctgta ttaatccagt
     4561 aatttgaatt atcaggaaac ccagttggag cctgataagc atatagtttt tgccccatat
     4621 tactcaccca attatacact gtaaacggcc ttttaacttc tgcatgtaaa actctaatac
     4681 tacttattgc taactcaaaa ggagatttta ctttttctct taaagcatcc ttattccaaa
     4741 attcaggaga tgaaaccatg gtcagtatta cttgtttaat at
//
LOCUS       DAOQPK010000169         4779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_11156_length_4779_cov_4.679297, whole genome shotgun sequence.
ACCESSION   DAOQPK010000169 DAOQPK010000000
VERSION     DAOQPK010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11156_length_4779_cov_4.679297"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PLU36_08175"
     CDS             <1..266
                     /locus_tag="PLU36_08175"
                     /inference="COORDINATES: protein motif:HMM:NF025167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HMZ46763.1"
                     /translation="LIFGLIHGMGFASEFVALEGTGLSAALNLLYANIGIEVGQLLFV
                     IVVLVISFICLNILKLNRREYILFTSGAIFGVALEMALTRLPF"
     gene            277..2661
                     /locus_tag="PLU36_08180"
     CDS             277..2661
                     /locus_tag="PLU36_08180"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HMZ46764.1"
                     /translation="MKKVIVGIIMLLGTNAFSQNIQNNPNSNHGNKFEQLGIILPTPN
                     SYRAASGAPGGKYWQQRCDYNIVCELDEKKLTLTGFETLTYFNNSPDVLNYLWLQLDE
                     NQHSTINNANYQESNMLNTTLSVAELQRMEDAKKDNGYGTQITKVTDATGNLLSYTIN
                     KTMMRVDLKTPLKPGQQFVFKINWFYKMANRMSMGGRGGYEFFPEDGNHIFTMTQWFP
                     RLCVYSDFQGWQNHQFTGRGEFALTFGNYKVQITVPADHIVMSTGECNNYNQMLTATQ
                     QQRWQKAQTTNEPLEIVTLNEATKAEATKSSKKKTWIYTANNVRDFAWCSSRKFIWDA
                     MPVKIEGKKVMCMSAYPKEAYHLYRKFSTKAVAHTIKTYSDFTIPYPYPVAQSIEASN
                     GMEYPMICFNFGRTEKDGTYSEATKNGMIGVIIHEVGHNFFPMIINSDERQWSWMDEG
                     LNTFVEYLTEELWDNKFPSKRGPAFSITDYMRLPKDQLEPIMTNSENIIQFGPNAYSK
                     PATGLNILRETIMGRELFDFAFKQYTKRWAFKHPEPADFFRTMEDASGEDLDWFWRGW
                     FFSTDACDIALDSVKYATYDATPPTERTMKNKPRALDKPLVPLFDDISKIRNREDKKI
                     VFATDADTSLRDFYWRYARGIEPYDTTKYLPEIGNAAIMQPMDIATQEKYTNTHLYEL
                     SFINKGGLVMPIIVEFTFEDGTKQLEKIPAQIWRKNEHKVTKVFMTPQKAVSITLDPM
                     KETADIDESNNTWFAKNETVQPSKFKIFKSGPFNRRNMMGPQKNPMQAAQEKKN"
     gene            2733..3239
                     /locus_tag="PLU36_08185"
     CDS             2733..3239
                     /locus_tag="PLU36_08185"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HMZ46765.1"
                     /translation="MIKASNIKIATITDLEEIITIYNFAVLAKFETADITTINWTKRK
                     TWFKEHTATLYPIYVYKVNNKVLGWISISPYRKGRMALKNTVEVSYYIHPNFKNKGIG
                     SLLLAHAINACKTLNYYNLIAIVIDKNVKSIMLLKKYNFKKWGKLPNVAIFDNIQCSH
                     LYYGLALK"
     gene            3378..>4779
                     /locus_tag="PLU36_08190"
     CDS             3378..>4779
                     /locus_tag="PLU36_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46766.1"
                     /translation="MKKYYSTLTLLLIFIQLQAQKQTAAGLISLEPDGIVNIITDMTL
                     SPDNKTLFLTGYGGRAYLYDATEKGKIKPIWKNSSLSGLSTGAICSFSQDGKYIMIRG
                     LKSTIAKTRAVLFSNYPKSWQKDDDVCVLDAVTGNTIFSTPNAYAISISNNTAFVSDK
                     DGFKFFSLPDGKLTKTIKVEDNEYAAISPSGKYIVESWDADKQTFKDVPSIVHRRTEL
                     KNAYRAKKILVIYETSNLDKPIAYSNDEIDVVTNMQFSPDEKFVYLQMQLAGQENSTV
                     PNPYAFQRVNIATGEIDKSFGIKANYCKVDFENNKVTSLVAGGNIGFLKQVKVQDMNN
                     AEDLSYFETRYKFMKTSTMFSPIALVSKSSLAYVYYEKNLYEWDYKILKKYFKKGATA
                     TDEELAIMADSLLDASLEDPNSKISKEIKKKEISGNYIMDITITGPKGAVATIFCESD
                     EKTNIPMQNALKDIIKK"
BASE COUNT         1824 a          708 c          812 g         1435 t
ORIGIN      
        1 cattaatttt tggtttaatt catggtatgg gctttgcatc agaatttgtt gctttagaag
       61 gcacaggctt atcggcagca ctaaatttat tatatgctaa tattggtata gaagtagggc
      121 agcttttgtt tgtaatagtt gttttagtaa tttcatttat ttgtctaaat attctaaagc
      181 ttaaccgaag agaatatatt ttatttacaa gtggtgctat ttttggtgta gctttagaaa
      241 tggcactaac tcgtttacct ttttaaattt tttaagatga agaaagttat agtaggaatt
      301 ataatgcttt taggcactaa cgccttttcg caaaatattc aaaataatcc caatagtaat
      361 catggtaata agtttgaaca attaggcatt atactgccta cgcctaattc ctatagagca
      421 gcaagtggtg ccccaggagg taaatattgg cagcaaaggt gcgattataa tattgtttgt
      481 gaattagatg agaagaagct aacacttacc ggctttgaaa cattaactta ttttaataac
      541 tcgccagatg tattaaatta tttatggtta cagttagatg aaaaccaaca cagcactata
      601 aataatgcta attatcaaga gtctaatatg cttaacacta cactaagtgt agcggaatta
      661 caaagaatgg aagatgctaa gaaagataat ggctatggta cacaaattac aaaagtaacc
      721 gacgctacag gcaacttgtt aagctatact attaataaaa ccatgatgag ggttgattta
      781 aaaacacctt taaaacctgg tcagcaattt gtttttaaaa taaactggtt ttacaaaatg
      841 gctaatagaa tgtctatggg cggtcgtggt ggatatgaat tttttccaga agatggcaac
      901 catattttta caatgacaca atggtttcct aggctttgtg tgtatagcga ttttcaaggt
      961 tggcaaaatc atcagtttac aggcagagga gagtttgcat taacattcgg aaattataaa
     1021 gtacaaataa cagtacctgc agatcatatt gtaatgtcaa caggagagtg taataattat
     1081 aaccaaatgc taactgccac acaacaacaa cgttggcaaa aagcacaaac cacaaatgag
     1141 ccactagaaa tagtaacctt aaacgaagcc acaaaagccg aagctactaa aagcagtaaa
     1201 aagaaaactt ggatttatac agcaaataat gttagagatt ttgcatggtg tagctctcgt
     1261 aaatttattt gggatgctat gcctgtgaaa attgaaggta aaaaagtaat gtgtatgagt
     1321 gcctatccaa aagaagcgta tcacctttat cgtaaatttt ctaccaaagc agtagctcat
     1381 actataaaaa catactcaga ttttacaata ccatatcctt atcctgtagc acaaagtata
     1441 gaagcaagca atggaatgga gtacccaatg atttgcttca attttggaag aacagaaaaa
     1501 gatggaacct attctgaagc tactaaaaac ggaatgatag gagtaattat tcatgaagta
     1561 ggccataact tttttccaat gattattaat agcgatgaac gccaatggag ctggatggat
     1621 gaaggcttga atacttttgt tgaatactta accgaagagt tatgggataa taaattcccc
     1681 tcaaaaagag gccctgcatt ttctataaca gattacatgc gtttacccaa agaccaatta
     1741 gagcctatta tgaccaacag cgaaaatatt atacaatttg gtcctaatgc ttatagcaaa
     1801 cctgctacag ggttaaatat tttaagagaa acaattatgg gcagagagtt atttgatttt
     1861 gcatttaaac aatataccaa aaggtgggca tttaaacatc cagaacctgc cgactttttt
     1921 agaacaatgg aagatgcaag tggagaagat ttagattggt tttggcgagg atggtttttt
     1981 agtacagatg cttgcgatat tgcacttgat agtgtaaaat atgctactta tgatgcaacc
     2041 ccacctacag aaagaacaat gaaaaacaaa ccaagagcat tagataagcc acttgttcct
     2101 ttatttgatg atatttctaa aattagaaac agagaagata aaaaaattgt ttttgcaacc
     2161 gatgcagaca cttctttaag agatttttat tggcgatatg caagaggcat tgaaccatac
     2221 gatacaacaa aatatttacc agaaattggt aatgctgcca ttatgcaacc aatggatatt
     2281 gctacccaag aaaaatatac aaatacacat ttatacgaat taagctttat taataaaggc
     2341 ggcttggtaa tgcctataat agtagaattt acttttgaag atggcaccaa acaattagaa
     2401 aaaatacctg cacaaatttg gcgtaaaaat gaacacaagg ttactaaagt atttatgaca
     2461 ccccaaaaag cagtttcaat aacattagac cctatgaaag aaactgcaga tattgatgaa
     2521 tctaataata cttggtttgc taaaaatgaa acagtacaac ctagcaaatt taaaattttt
     2581 aaaagtgggc catttaacag aagaaatatg atggggcctc aaaaaaatcc catgcaagct
     2641 gctcaagaaa agaagaatta attcaaaaaa aaagtaaaaa tattggctca ccaaattata
     2701 aatagaagaa atatttacaa cctttaaact gcatgattaa agcaagtaat attaaaattg
     2761 ctacaattac tgatctggag gagataataa ctatttacaa ttttgcagtt ttagcaaaat
     2821 ttgaaacagc cgatataaca accataaatt ggacaaaaag aaaaacctgg tttaaagagc
     2881 atacagcaac cttataccct atttatgttt ataaagtaaa taataaagtg ttaggctgga
     2941 taagtattag cccctacaga aaagggcgaa tggcgttaaa aaacactgta gaagtaagtt
     3001 actatataca ccctaatttt aaaaataaag gaattggatc tttattgtta gcccatgcaa
     3061 taaatgcatg taagacatta aattattata acttaatagc tattgtaata gataaaaatg
     3121 taaaaagcat aatgcttctt aaaaaatata attttaaaaa gtggggcaaa ttacccaatg
     3181 ttgccatatt tgataatata caatgtagtc atttgtatta tggtttagct ctcaagtaaa
     3241 aattaacttt tacccaaaca aacttctaaa taaaacattt aacgcttagg gttaaattct
     3301 tttagttata caaaataccc aattggcggt attttttttt tgtgaactcc ttctattttt
     3361 attcaataat ttttaaagtg aaaaaatatt attcaactct aaccttgtta ttgatattta
     3421 ttcaattaca ggctcaaaaa caaactgctg caggcttaat aagtttagag ccagatggca
     3481 ttgtaaatat tataactgat atgacattaa gcccagataa caaaacctta tttttaacag
     3541 gttatggcgg aagggcttat ttgtatgatg ctacagaaaa aggtaaaata aaacccattt
     3601 ggaaaaattc ttctttaagt ggattaagta caggagccat ttgttctttt agtcaagatg
     3661 gtaaatatat aatgatacga ggactaaaaa gtacaatagc taaaacaagg gctgtactat
     3721 tttccaatta tcctaaaagc tggcaaaaag acgatgatgt gtgtgtgcta gatgccgtta
     3781 caggcaatac aatattttct acacccaatg cttatgcaat aagtatatca aataatacag
     3841 catttgtaag cgataaagat ggatttaagt ttttctcctt acccgatggt aagctaacta
     3901 aaactattaa agtagaagat aatgaatatg cagctataag cccaagtgga aagtatattg
     3961 tagaaagttg ggatgcagat aaacaaactt ttaaagatgt tccatctatt gtacatagaa
     4021 gaacagaatt gaagaatgca taccgagcca aaaaaatttt agtgatctat gaaacttcta
     4081 acttagataa acccattgca tatagtaacg atgaaataga tgtagtaacc aatatgcaat
     4141 ttagccctga tgaaaaattt gtttatttac aaatgcaatt agctgggcaa gaaaattcta
     4201 ctgtacccaa cccgtatgct tttcaacgag taaacattgc aacaggagaa attgataaat
     4261 cgtttggtat aaaagccaat tactgtaaag tagattttga aaataataaa gtaactagcc
     4321 ttgttgcagg cggcaatatt ggttttctaa aacaagttaa ggttcaagat atgaataatg
     4381 cggaagattt atcttatttt gaaacaagat ataaattcat gaaaacatct actatgtttt
     4441 cgccaattgc cttggtttca aagtcttctc ttgcttatgt ttattacgaa aagaatttat
     4501 acgaatggga ttataaaatt ttaaaaaaat actttaaaaa aggggcaact gcaaccgatg
     4561 aagagttagc tattatggca gattctttat tagatgctag tttagaagac cccaactcta
     4621 aaatttcaaa agaaataaaa aagaaagaaa tatctggcaa ctacataatg gatattacca
     4681 ttacaggccc taaaggtgct gtagctacaa tattttgcga aagcgatgaa aaaacaaata
     4741 tccctatgca aaatgcctta aaagatatta ttaaaaaac
//
LOCUS       DAOQPK010000170         4762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_11214_length_4762_cov_5.002549, whole genome shotgun sequence.
ACCESSION   DAOQPK010000170 DAOQPK010000000
VERSION     DAOQPK010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11214_length_4762_cov_5.002549"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /locus_tag="PLU36_08195"
     CDS             <1..1141
                     /locus_tag="PLU36_08195"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HMZ46767.1"
                     /translation="TTTEAPATFVAAPVAPVVQEVVAPLPTQTIENELPYLPSDNATK
                     QVGGGNNKFYSPLVLNIANSEGISLTELENIPGTGNEGRVSKRDILQYVADKKAGKVI
                     SPVTTVQPIAVATTTIVTPEVTTTAPTAVKEQPAQPAVVINGNVEIIEMDRMRKLIAK
                     HMVDSKHTSPHVTSFTEADVTNMVLWRDRVKKQFEKEVGTKITFTPMFIECITRVMKR
                     YPMINASIDGDRIIIKKDFNIGMATALPSGNLIVPVIKGADMLNLVGLSKTVNNLADC
                     ARNNKLKPDDTQGGTFTLTNVGTFGSLMGTPIINQPQVAILAVGAIKKRPVVVETPEG
                     DTIGIRHMMYLSMSYDHRIVDGSLGASFLTAVAKEFEKWDMHREW"
     gene            complement(1228..1782)
                     /locus_tag="PLU36_08200"
     CDS             complement(1228..1782)
                     /locus_tag="PLU36_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46768.1"
                     /translation="MENYYKLISFEIEPSIDNRRKIINYFTLNGFNIIEENFEKIVCN
                     KKDSFLSSWHFNPLKWSSNVVINLFKNSIEAHFSLDITNINNPKQEIAVWHIFLQNFQ
                     HTLQGKEINTTELNLAIKQSKKTGWLNFCKFIFIYTIVGLIGFYSSKSFGLKYPLFFS
                     LPLIIILTAIFNKKRIEKQLKAFT"
     gene            complement(1784..3049)
                     /locus_tag="PLU36_08205"
     CDS             complement(1784..3049)
                     /locus_tag="PLU36_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HMZ46769.1"
                     /translation="MNKIFATIITIGDELLIGQVIDTNSAFIAQELNKIGISVKQRVS
                     IADEEEAIFTALDNAFIHSNIILITGGLGPTSDDITKPTLCKYFGGKMTMHQPTLQHV
                     TYLFEQVFKRPMIERNVQQAMVPDVCTVLKNEVGTAPAMLFKKNNTICIAMPGVPFEM
                     KWIMQHHVLDIIPQQFKTGIILHKTLLTAGIGESFLAELIQDWENKLPTDIKLAYLPK
                     FGMVRLRLTGLGLNKELLAEKMQTEFEKLKTIVQEFLVIDEDLSLEVVVSNLLKQYHQ
                     TVAIAESCTGGAIASSITALPGASSVFKGGIVCYSNYAKNKLLHVPNEILNTVGAVSK
                     ETVEILATNILKNHNSHYALAVSGIMGPTGATETKPIGLVWIAVVSEKNLITKQFNFK
                     YDRERNIQHTTVNALNMLRKLIIEENKNS"
     gene            complement(3066..3272)
                     /locus_tag="PLU36_08210"
     CDS             complement(3066..3272)
                     /locus_tag="PLU36_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HMZ46770.1"
                     /translation="MRYACLIVLFFILFFLRAQEPTLVTLIGHSETITLTKFSPNERL
                     MLTASEDALYKLNFATTGNKKVLV"
     gene            complement(3343..>4762)
                     /locus_tag="PLU36_08215"
     CDS             complement(3343..>4762)
                     /locus_tag="PLU36_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HMZ46771.1"
                     /translation="SEFYTIANNLNNCYFTTYTGNKPVEILNNHLYKADKSAINNLAF
                     VLKEKGFLPEDFDMYFNRPDSVTFEAGSTNATYNELLSKVVEKRKRKFNTQNITEVLS
                     NSPTCTIINKNEIPDVTKENHILLQIKASSPTSAAITAIHILNNGVPVFGKAGFTNNN
                     QPITEEQKIDVVLTNGINNVQVYVSDNTALVSASESIIISSDYIEVTEPKQHFIGIGI
                     NQFSNPDYNLNWSVKDIRDLALKLKSKYPNIIIDTLFDENVTKEKILAVKKKLLQLNE
                     NDKVIVSYSGHGVLNKDFDYFLSTYSINFEKPEEKGLSYNDLENLLDSIKPRQKLMFI
                     DACHSGEVDKEEIEKVEASKKELEKNGVVTKSTIKVKPKKNIGMANSFELMQNLFVNV
                     GKGTGATIISAAGGMQYAQERGDLKNGVFTYSVLEAFDKNTTLKVSELKKIVGERVIQ
                     LTNGLQKPTSRNEANNYDWVVW"
BASE COUNT         1537 a          739 c          737 g         1749 t
ORIGIN      
        1 cactacaaca gaggctcctg caacttttgt tgcagcacca gttgcccccg ttgtacaaga
       61 agttgttgca cctttaccaa cgcaaactat agaaaatgaa ttaccttatt tgccatctga
      121 taatgcaacc aaacaagtag gtggtggaaa caataaattt tactcaccat tggttttaaa
      181 tatagccaac agtgaaggta taagtttaac cgaacttgaa aatataccag gcacaggtaa
      241 tgaaggaaga gttagtaaaa gagatatttt acaatatgtt gctgataaaa aagcaggtaa
      301 agtaatatcg ccagttacta cagtacaacc aatagcagtt gctacaacta ctatcgtaac
      361 accagaagta acaactacag caccaactgc agttaaagaa caacctgcac aacctgctgt
      421 agttataaat ggaaatgtag aaattattga aatggataga atgcgtaaac taatagccaa
      481 acacatggtt gatagtaagc atactagtcc gcatgtaact agttttacag aagctgatgt
      541 taccaatatg gttttgtgga gagatagagt taaaaagcaa tttgaaaaag aagtaggaac
      601 taaaattact tttacaccaa tgtttattga gtgcattaca agggttatga aacgttaccc
      661 tatgattaat gccagcattg atggagatag aattattata aagaaagatt ttaatattgg
      721 catggcaact gccttgccat cgggaaattt aattgtgcct gttattaaag gggctgatat
      781 gctaaactta gttgggttaa gtaaaacagt aaataattta gcagattgtg ccagaaataa
      841 taaacttaaa ccagatgata cacagggtgg tacttttacc ttaaccaatg taggcacttt
      901 tggaagttta atgggaacgc ccattattaa ccagcctcag gttgctatac ttgctgtcgg
      961 tgcaattaaa aaacgcccag tagtagttga gactccagaa ggagatacca ttggtataag
     1021 gcacatgatg tatttaagta tgagctacga tcatagaatt gtagatggta gtttgggggc
     1081 aagtttttta acggctgttg ctaaagaatt tgaaaaatgg gatatgcata gagagtggta
     1141 attaaacctg atattttttt aagccattta atttaaaagt tgaatggctt tttttatttt
     1201 catttatgtt taaaaatgta tgagcggtta agtaaaagct tttaattgtt tttctattct
     1261 ttttttatta aatattgctg taagaataat aataagaggc aaagaaaaaa ataaaggata
     1321 ttttaacccg aatgatttac tgctatagaa accaattaaa ccaacaatag tgtaaataaa
     1381 aataaactta caaaaattta accatcctgt tttttttgat tgtttaattg caaggtttaa
     1441 ttcagttgta tttatctctt taccctgtaa tgtatgctga aaattttgta aaaaaatatg
     1501 ccaaactgca atttcctgtt taggattatt gatgtttgtt atatctaaag aaaaatgagc
     1561 ttcaatacta ttcttaaata aattaataac cacatttgaa ctccatttta gaggattgaa
     1621 atgccaactg cttaaaaaag aatctttttt attacataca attttttcaa aattctcttc
     1681 aataatgtta aaaccattta aagtaaagta attaatgatt tttctacgat tatcaattga
     1741 tggctctatt tcaaaagaga taagtttata ataattttcc atttcagcta tttttatttt
     1801 cttctataat taattttctt agcatattta aggcatttac agtggtgtgt tgtatatttc
     1861 tttctctgtc gtatttaaaa ttaaattgtt tagttattaa atttttttcg gatactactg
     1921 ctatccaaac aagtccaata ggctttgttt ctgttgcacc agttggtccc ataattccac
     1981 ttactgcaag tgcataatgg ctgttatgat tttttaatat attagttgct aaaatttcta
     2041 ccgtttcttt acttacagca ccaactgtgt ttaatatttc atttggcaca tgtagtaatt
     2101 tatttttagc gtagttgcta taacaaacaa tgccaccttt aaaaacacta ctagcaccgg
     2161 gtaatgcagt aattgaactt gcaatggcac cacctgtaca actttctgca atagctactg
     2221 tttggtgata ttgtttgagc aggttagata ctactacttc taaacttaga tcttcatcaa
     2281 ttactaaaaa ttcttgaaca atagttttta atttttcaaa ttcggtttgc attttttctg
     2341 ccagcagttc tttgtttaag cccaatccag ttaaccttaa gcgtaccata ccaaattttg
     2401 gcaggtaagc taattttata tcagttggta atttattttc ccaatcttga attagctctg
     2461 ctaaaaagct ttcgccaata ccagctgtta gtaaggtttt atgtaaaata attcctgttt
     2521 taaattgttg tggtataata tctaaaacat ggtgttgcat aatccatttc atttcaaaag
     2581 gtacacctgg cattgcaata caaatggtat tatttttttt gaaaagcatt gctggtgcag
     2641 taccaacttc atttttaagc actgtacaaa catctggcac cattgcttgt tgtacgtttc
     2701 gttcaatcat tgggcgttta aaaacctgtt caaataaata ggttacatgt tgtagagtag
     2761 gttggtgcat ggtcattttt ccgccaaaat atttgcataa agttggtttg gttatatcat
     2821 cggatgttgg gcctaagcca ccagttatta gaataatatt tgaatgaata aaagcattat
     2881 ctaatgcagt aaaaatggct tcttcttcat cggctatgga aactctttgt tttacggaaa
     2941 tgcctatttt atttaattcc tgagctataa atgcactatt ggtatctatt acctggccaa
     3001 ttaataattc atcgcctatt gtaattattg ttgcaaaaat tttattcata aacttaaaaa
     3061 aataattaaa ctaaaacttt tttgttgccg gtagttgcaa agttaagttt atacaaggca
     3121 tcttcagatg ctgtaagcat caatcgttca tttggtgaaa attttgtaag agtaatagtt
     3181 tctgaatgcc caataagagt tactaaagtt ggttcttgtg ctcttagaaa aaataggata
     3241 aaaaataata ctattaagca tgcatatctc atgaaaaatt tttgtacaaa aaactatttc
     3301 tgcaaaaaat gaaatataat taataaaaat cagcaatacc atttaccaca ctacccaatc
     3361 ataattatta gcttcatttc ttgatgttgg tttttgtaaa ccattggtaa gttgtattac
     3421 acgttcacct actattttct ttaattctga aacttttaat gtagtgtttt tatcaaatgc
     3481 ttccaaaaca ctgtatgtaa atacgccatt ctttaaatcg cctctttcct gtgcatattg
     3541 cataccacct gcagcactaa taatagttgc tcctgtgcct ttgcctacat ttacaaacaa
     3601 gttttgcatg agttcgaaac tatttgccat gcctatgttt tttttaggct ttactttaat
     3661 agtgctttta gttacaactc catttttttc taattctttc ttagatgctt caaccttttc
     3721 tatctcctct ttatctactt ctccactatg gcaggcatct ataaacatga gtttttgacg
     3781 tggctttata ctgtctaaca aattttccaa atcattgtaa gacaaacctt tttcttctgg
     3841 tttttcaaaa ttgatactat acgtggaaag aaaataatcg aagtctttgt ttaatacacc
     3901 atgaccactg tatgatacaa ttactttatc attctcattc agttgcaata attttttctt
     3961 tactgctaaa attttttctt tggttacatt ttcatcaaac aatgtatcta taataatgtt
     4021 tggatatttt gattttagct tgagtgccaa atctctaatg tcttttacac tccagtttaa
     4081 attgtaatct ggattggaga attgattaat gcctatgcct atgaagtgtt gttttggttc
     4141 tgttacttct atataatctg aactgatgat aatactttct gatgcagaaa ccaatgctgt
     4201 attgtctgaa acatacactt gaacattatt aattccattg gttaaaacaa catcaatctt
     4261 ttgttcttct gttattggtt gattattgtt tgtaaaacca gctttgccaa aaacaggtac
     4321 accattgttt aaaatatgta tagctgtaat tgctgcagat gtaggtgaac ttgcttttat
     4381 ttgcaacaga atatgatttt cttttgttac atcgggaatt tcatttttat taattattgt
     4441 acaagtagga ctattgctta aaacctctgt aatgttttgt gtgttgaatt ttctttttct
     4501 tttttctacc actttactca ataattcatt ataagttgca tttgttgaac ctgcttcgaa
     4561 tgtaacagaa tcaggtctgt taaaatacat gtcaaaatct tcgggtaaaa agcctttttc
     4621 ttttaacaca aatgctaagt tgttgatggc agatttatct gctttataca aatggttgtt
     4681 taaaatttct actggtttat tacctgtata agttgtaaaa taacagttat ttaaattatt
     4741 ggcaattgta taaaattcac tt
//
LOCUS       DAOQPK010000171         4743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_11305_length_4743_cov_3.285623, whole genome shotgun sequence.
ACCESSION   DAOQPK010000171 DAOQPK010000000
VERSION     DAOQPK010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4743
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11305_length_4743_cov_3.285623"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /locus_tag="PLU36_08220"
     CDS             <1..1261
                     /locus_tag="PLU36_08220"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HMZ46772.1"
                     /translation="LYFFSLLLVNIFLSRSLQADGTGWIYYLTNFFSFILLVASLSME
                     SGYTYYAAGNIISHHKLAWFSLCWTLLICIISAAGVGIYFKSIKGIDTFYTDNFIYYA
                     ATYISGILLINFFMVLFYAQKNYATPNILLSVINFLLIIIILLNPKSYNIVVNSYFYS
                     ILTQGILLAIAFIYLNKSFQLISLPDKSSLKLLLNYSLVALTSNVIFFFVYRIDYWFV
                     KYNVESCTPDDLGNYIQASKLGQMMLIVPQIFASVIFPHTASGASKNNITESIHIFFR
                     IFLQVLIVALIVVSIIGNWLFPAVFGITFNKMTMPVLLLLPGIFSLSVLCLLSAYFGG
                     VGKVKINVIGGVYALIIVIIGNLIFIPLYGIIGAAIVSSIGYSVNLVYSFYHFFKDNP
                     TSIKTLFIFKRSDWVWVKKMLFHKSTT"
     gene            1261..2430
                     /locus_tag="PLU36_08225"
     CDS             1261..2430
                     /locus_tag="PLU36_08225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ46773.1"
                     /translation="MKIIWLASWYPNKYEPVNGDFIQRHAKAVSNILPIDVLHVVQLG
                     KNFEANNEIETINAKNLTEHICYFKFKKWDIGIIDKIRYNTLYKKTALAFVENFIKTN
                     GKPDLIHVHVPMKSGWVAYSLKKKYSIPYIVSEQASYYDDEAPDNFKNRSLFFKLNTK
                     KVCKNAIAISNVSSVIAKKMEKLLNVKNIHTIHNIANTKRFFYMPIQPQNEFIWVHVS
                     TLSEQKNIPGIIYAFRQLVAENIQLCKLVLVGTYKELHEPLVKQLQLEHLINFTGEVT
                     HHQVAGYLQSANAFVLFSKHENFPCVLVEALCCGLPVVATNVGGIADAINDNNGLMVP
                     SNNVVALTAAMKSVMMNYKNYNQAFIAERAKQLYDDEVIAKQFVALYENCLKDKV"
     gene            complement(2427..3287)
                     /locus_tag="PLU36_08230"
     CDS             complement(2427..3287)
                     /locus_tag="PLU36_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HMZ46774.1"
                     /translation="MQTFNQINIVECPRDAMQGWKNFIKTEDKIRYINSLLKVGFHTL
                     DFGSFVSPKAIPQMADTKEVIKGLQINNHTKLLAIVANIRGAEDAVIFDEITYVGFPF
                     SISPTFQQRNTNSSLEESIERLETIQELCIKNKKQLVVYLSMGFGNPYGDEYNQEILL
                     QWANEMVKREINIISLADTVGLATPQQISFALNTLIPKYTNTTIGVHLHSSPYNFKEK
                     IEASVNAGCKRFDGALKGIGGCPMAQDSLVGNMNTELMITYFEEENLINNINKDALKE
                     SLLIAQEIFV"
     gene            complement(3385..4494)
                     /locus_tag="PLU36_08235"
     CDS             complement(3385..4494)
                     /locus_tag="PLU36_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HMZ46775.1"
                     /translation="MANINYRHFTLGVEEEYMVIDPTTRELKSHEQKIVQEGQKLIKD
                     KVKAEMHQAVVEVGTDICYDIDEAFKDVSTLRRTISQIAGDLGLWVGASGTHPFSHWE
                     SQLITEHVRYNEIVNELQEAARSNLIFGLHVHVGMEDRKMAIHIANSARYFLPHVYAL
                     STNSPFWEGRKTGYKSFRTKVFDKFPRTGIPDYFDSIEAYDNYVNLLIKTHCIDNAKK
                     IWWDLRVHPFFNTVEFRICDVPLTVTETIAIAALFQAICAKLYKLRSENLNFIIYQRA
                     LLNENKWRAGRYGIDGKMIDFGKESEVNTRLLIYELLDFVDDVVDDLGSRPFITYIQK
                     MLEQGTGADRQLAVFEETKDLVKVTDYIHDQFLSL"
BASE COUNT         1515 a          702 c          689 g         1837 t
ORIGIN      
        1 gttatacttt ttttctttat tattagtaaa tattttttta agcagaagct tacaagcaga
       61 tggcacaggt tggatatatt atttaactaa ctttttttct tttattttat tggttgcaag
      121 tttaagtatg gaaagtggat atacttacta tgctgctggc aatattatca gccatcataa
      181 acttgcctgg ttttcgttgt gttggacctt attaatttgt attatttcag cagcaggtgt
      241 aggtatttat tttaaaagta taaaaggaat tgatactttt tataccgata attttattta
      301 ttatgctgct acttatattt cgggcatttt gctaattaat ttttttatgg ttttatttta
      361 cgctcaaaaa aattatgcta cgccaaatat tttattaagt gttataaact ttttgctcat
      421 tataataatt ttattgaatc caaaatcgta caatattgta gttaatagct atttctacag
      481 catattaaca caaggtattt tattggctat tgcatttatt tatttgaata aatcttttca
      541 actaatttct ttgccagata aatcgtcatt aaaattattg ttgaattact ctttagtagc
      601 tttaactagt aatgtaattt tcttttttgt atatagaata gattattggt ttgtaaaata
      661 caatgttgaa agttgtacac cagatgattt aggtaattat attcaagcat caaagcttgg
      721 acaaatgatg ttaattgtgc cacaaatttt tgcaagtgtt attttcccac atacagccag
      781 tggagcttcc aaaaataata ttacagaatc tatccatatt tttttcagaa tatttttaca
      841 agtattaatt gttgctctta ttgtagtaag tattattggt aactggcttt tccctgctgt
      901 gtttggcatt acttttaata aaatgactat gcctgtttta ttattacttc ctggtatatt
      961 ttctttatct gttttatgtt tattgagtgc ttattttggt ggtgttggta aagttaaaat
     1021 taatgttatt ggtggggttt atgctttaat tatagtaata ataggaaatt taatttttat
     1081 tcctttatat ggaattattg gtgctgccat tgtaagcagt attggctatt ctgttaattt
     1141 agtatattct ttttatcact tttttaaaga taatcctaca agtataaaaa cgttatttat
     1201 ttttaaaaga agcgattggg tgtgggtgaa gaaaatgctt tttcataaat ctactacata
     1261 atgaaaatta tttggcttgc aagttggtat cctaataaat atgaaccagt aaatggcgat
     1321 tttattcaac gccatgcaaa agctgtttct aatatattac ctatagatgt attgcatgta
     1381 gtgcaattag gaaaaaattt tgaggctaat aatgaaattg aaactattaa tgcaaaaaac
     1441 cttacagaac atatatgtta ttttaagttt aaaaaatggg acattggtat aattgataaa
     1501 ataagataca atactttata taagaaaacg gctttagctt ttgttgaaaa ttttattaaa
     1561 acaaatggta agcctgattt aatacatgtt catgtgccca tgaaatctgg ttgggtagca
     1621 tattcattga aaaaaaaata ttccattcca tatattgtgt ctgagcaggc ttcttattat
     1681 gatgatgaag caccagataa ttttaaaaac agaagtttgt tttttaaatt aaatacaaaa
     1741 aaagtttgta aaaatgcaat tgctatcagc aatgtatcta gtgtgattgc taaaaaaatg
     1801 gaaaagttgc tgaatgtgaa aaatattcat actattcata acattgctaa tacaaagcgt
     1861 tttttttata tgcctattca accacaaaat gaatttattt gggtacatgt ttcaacctta
     1921 agtgagcaga aaaatatacc tggtattatt tatgcttttc gtcaattagt agctgaaaat
     1981 attcagcttt gtaagttggt attagttggt acatataaag aattacatga gcctcttgta
     2041 aagcagttgc aactagaaca tttaattaat tttaccggag aagttacaca tcatcaagtg
     2101 gcaggttatt tacaatctgc aaatgctttt gtgctattta gtaaacacga aaattttcca
     2161 tgtgtattag tagaagcttt gtgttgtggt ttgccagttg ttgccactaa tgttggtggc
     2221 atagcagatg ctataaatga caataatggt ttgatggtgc catctaataa tgtggtggca
     2281 cttactgctg ctatgaaaag tgtaatgatg aattataaaa attataacca agcattcatt
     2341 gcagaaagag caaaacaact ttacgatgat gaagtaattg ctaaacagtt tgttgctttg
     2401 tatgaaaatt gtttaaagga taaagtttaa acaaaaattt cctgagcaat taataaactt
     2461 tcttttaaag catctttatt tatattattt ataagatttt cttcttcaaa ataagtaatc
     2521 attaactcag tgttcatatt gcctactaaa ctatcttgtg ccattgggca accaccaatt
     2581 ccttttaaag ccccatcaaa tcgtttacac cctgcattaa ccgatgcttc aattttttcc
     2641 ttaaagttgt aaggcgatga atgtaaatga acaccaattg ttgtattggt atatttagga
     2701 ataagtgtat ttaaagcaaa acttatttgc tgaggtgtgg ctaaaccaac agtatcagcc
     2761 aatgaaatta tattaatttc tctttttacc atttcatttg cccattgcaa taaaatttct
     2821 tggttatatt catcaccata aggattgcca aaacccatac ttaaatacac aactaattgt
     2881 tttttatttt taatacacaa ctcttgaatg gtttctagtc tttctatact ttcctctaag
     2941 ctgctattgg tattacgttg ctgaaatgta ggagaaatgg agaaaggaaa gcctacataa
     3001 gttatttcat caaaaataac tgcatcttca gcacctctta tattagctac aatggctaat
     3061 agttttgtat ggttatttat ttgtagccct tttattactt ctttggtatc tgccatttga
     3121 ggtattgctt ttggtgaaac aaaacttcca aaatctaaag tatgaaaccc aacttttaat
     3181 aagctgttga tgtatctaat tttatcttct gttttaataa aatttttcca accttgcata
     3241 gcatctctgg gacattcaac aatattaatt tgattaaatg tttgcatttt ttttgcctgt
     3301 ttaaaggtgt aaaagtattt acttattgca taaaaaaaca gttaataaaa atattaactg
     3361 ttttaaattt tttggtaaaa ctaattacaa acttaaaaat tggtcatgaa tataatctgt
     3421 tactttaact aagtcttttg tttcttcaaa aacagccaac tgtctatctg cacctgtgcc
     3481 ttgctctaac attttttgaa tgtaagttat aaatggtctg ctacctaaat catctactac
     3541 atcatccaca aaatctaata actcgtatat cagcaacctt gtatttactt cagattcttt
     3601 tccaaaatct atcatttttc catcaatacc atatctgcca gctcgccact tgttttcatt
     3661 taataaggct ctttgataaa taataaaatt caaattttcc gatcttagct tatatagttt
     3721 tgcacaaata gcctgaaata atgcagcaat agcaatggtt tcggttacag taagtggcac
     3781 atcgcaaatt ctaaactcaa cggtattaaa aaaaggatgc actcttaaat cccaccaaat
     3841 ttttttagca ttatctatac aatgtgtttt tataagaagg tttacataat tatcgtatgc
     3901 ttcaatgctg tcaaaataat ctgggatacc tgtacgagga aatttatcaa aaacttttgt
     3961 tctaaaagat ttgtatcctg tttttcttcc ttcccaaaaa ggcgaattgg tacttaaagc
     4021 atatacgtgt ggcaaaaaat atctggcact attggcaata tgaatggcca tttttctatc
     4081 ttccatacct acgtgtacat gcagcccaaa aattaaatta cttcttgcag cttcttgtaa
     4141 ttcgtttaca atttcattat agcgtacatg ctcagtaatt aattggcttt cccaatgact
     4201 gaaagggtgt gtaccacttg caccaaccca taaacctaaa tctcctgcaa tttggctaat
     4261 tgttcttctt agtgtagata catctttaaa agcttcgtct atatcataac aaatgtctgt
     4321 accaacttct acaacagcct gatgcatttc tgcttttact ttatctttta ttagtttttg
     4381 accttcttgt actatttttt gctcatgact ttttaattca cgtgttgtag ggtctattac
     4441 catgtattct tcctctactc ctaaagtaaa atgtctgtaa ttaatatttg ccatttagtt
     4501 gaataagttt taagtaaata atagctgctt tttaataaaa tatttattcc gtttctttat
     4561 ttaaaataaa ttatttggtt tagtataaag ttcttttagc tgcactgtgt tgtatgtatt
     4621 taccccaagt taaattatct tttccgggct cgaacgactg tgctttttca attgcatatt
     4681 ttgaagcagt ttctactacc catgcaaaat tatcttcccc aacactattt ttatctgcat
     4741 ctg
//
LOCUS       DAOQPK010000172         4709 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_11440_length_4709_cov_4.563601, whole genome shotgun sequence.
ACCESSION   DAOQPK010000172 DAOQPK010000000
VERSION     DAOQPK010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11440_length_4709_cov_4.563601"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1795)
                     /locus_tag="PLU36_08240"
     CDS             complement(<1..1795)
                     /locus_tag="PLU36_08240"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HMZ46776.1"
                     /translation="MYTNKLISETSVYLLQHAHNPVNWFTWGEEAFKLAKEENKPILL
                     SIGYAACHWCHVMEKESFEDVETASLMNKYFINIKIDREERPDIDAIYMDAVQAITGS
                     GGWPLHVFLTPDKKPFYGGTYFPPERKYNRLSWKEVLTNIADAYTNKYNEIEEQANKL
                     TQHLQQSNFLNFQNLNLEEEQIFNEETDFEIASTILKSADMVDGGFGNAPKFLQTYSI
                     QYLLRHYHFTKNIDALNHAVLSLNKMISGGIYDQLGGGFSRYSTDAQWHAPHFEKMLY
                     DNALMVNLLSEAYQLTNNKDYKRIIEETLEFVLSEMTAKNEGFYTAFDADSEGEEGKF
                     YTWTKAEIENVLKEKAKLFCSYYNITERGNWENTNILFAKHSIAEFAQKQNITENELS
                     IFLIECKKILFEIRVNRIKPNLDDKVLLGWNALMITAFCKAFAATGNLKYKQTAIEKI
                     YFIENNLKVNSQWKHTYKNGKSKIDAFLDDYAYLIEAYIYLQEISADSKYLIIAKQLT
                     QHVLKNYSSNSIFFNYTNQNQPDILVHKQDVYDGALPSANAIMAKNLDYLGIVFSIQE
                     WQNIANKMLQNMVPSIINHPNSFAVWASLVQQ"
     gene            complement(1893..2687)
                     /locus_tag="PLU36_08245"
     CDS             complement(1893..2687)
                     /locus_tag="PLU36_08245"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HMZ46777.1"
                     /translation="MAKIGAKSTVPTKAVKRILISQPRPESEKSPYFDLERKYDVQLF
                     FTPFIKLDAITAKEFRKQKIDIASYSAVVFTSRNAIDFFFKMCEEMKVPVSQDTKYFC
                     ITEAVALYLQKFTLYRKRKVFYGADGSNKSLFDVINKHKENERFMYVCSENQQDNEIV
                     NWFKNHHCDFTLAFMYRTISNDIKEFFKKTSYDVICFFTPSGIKSLFDNIPEFKQKNT
                     EIGVFGNNTLLAAENAGLKTLIKAPIPQVPSMITALDKYLAEKNKN"
     gene            complement(2708..3691)
                     /locus_tag="PLU36_08250"
     CDS             complement(2708..3691)
                     /locus_tag="PLU36_08250"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HMZ46778.1"
                     /translation="MLLLNCNFLFAQVDTSKIDTLKTQIKDTTKKKSKPLVKPKTISD
                     SILHITDTIISTQDSVKSNSPDSAIIKIAEPKKLLIDTSTFESLFSSKYYPVNGSRIF
                     MIDVKRKAENNHFLFYLLIGTLLFASLIKAIFSKYFSDIFKIFFQSSFRQKQTREQLL
                     QDSLPSLLMNILFFISSSIFITLIAKQYHLLIENFWLTLLYSTILITSVYFFKYIFLS
                     FIGWVFNIAKTINAYVFTVFIINKLLGVILIPVILIMAFSLHNIAYVSLIIALILIAI
                     LFIYRYWLGIKSVRNSFKVSALHFFLYLCAVEVLPMVLLYKAVFNVFKSNL"
     gene            3894..4520
                     /locus_tag="PLU36_08255"
     CDS             3894..4520
                     /locus_tag="PLU36_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46779.1"
                     /translation="MKRITILFMSASLLLSFTVTQAQETGGKRRGKLEKREKMAGDKK
                     AGFGKDLNLTEEQKAEAKKINEEYNAKVKALKSNDKITMGEYNKQMAALQKERREKLE
                     AILTPEQKEKVESRKQDKEDKMQKREEMVLEKMKENLNLSDDQVTKIKTINETYAKKA
                     QAVRNDKNLSQEQKKQALLAIAKERKEAIGNTLTTEQKEKQKNLRNRD"
BASE COUNT         1719 a          656 c          675 g         1659 t
ORIGIN      
        1 tttgttgtac taaagatgcc caaactgcaa atgaatttgg gtggtttata atggatggaa
       61 ccatattttg aagcatttta ttggctatgt tttgccattc ctgaattgaa aaaacaattc
      121 ctaaataatc taagtttttt gccattattg catttgcact tggtaatgca ccatcgtaca
      181 catcttgctt atgcactaaa atatctggct gattttggtt ggtataatta aaaaatattg
      241 aattgctact ataatttttt aaaacatgtt gggttaactg tttggcaatt attaaatatt
      301 tactatcagc cgatatttct tgtaaataaa tataagcttc aattaaataa gcataatcat
      361 ctaaaaaagc atcaattttt gatttgccat tcttataagt atgtttccat tgactgttaa
      421 cctttaaatt attttcaata aaatatattt tttctatggc tgtttgcttg tatttaaggt
      481 tgcctgttgc tgcaaatgct ttacaaaatg cagttatcat taatgcattc caacccagta
      541 gaaccttatc gtctaaattt ggttttatcc tatttaccct tatttcaaat aatatttttt
      601 tacattctat taaaaaaata cttagttcat tttcagtaat attttgtttt tgtgcaaatt
      661 ctgcaataga gtgttttgca aataatatat tagtgttctc ccagttacct ctctcggtta
      721 tattataata ggaacaaaaa agcttggctt tttcttttaa aacattttca atttcggctt
      781 tagtccaagt ataaaatttg ccttcttctc cttcactatc agcatcaaaa gcagtataaa
      841 atccttcgtt tttggctgtc atttcggata atacaaactc taaggtttcc tctataattc
      901 ttttataatc tttattattt gttaattgat atgcttcgga taatagattt accattagag
      961 cattatcgta taacattttt tcgaaatgag gtgcatgcca ctgtgcatct gtactgtatc
     1021 ttgaaaagcc tccacccaat tgatcataaa tacctcccga tatcatttta tttaggctta
     1081 aaacagcatg gtttaaggca tcaatgtttt ttgtaaaatg atagtgcctt aacaaatatt
     1141 gtatagaata agtttgaaga aatttaggag catttccaaa accaccatca accatatcgg
     1201 cagattttaa aatggttgag gctatttcaa agtctgtttc ttcattaaaa atttgctctt
     1261 cttctaaatt tagattctga aaattgagaa aattagattg ttgtaagtgc tgtgtaagtt
     1321 tatttgcttg ttcctctatt tcattatact tattagtata agcatctgca atattagtaa
     1381 gtacttcttt ccagcttaat ctattatact ttctttcagg agggaaataa gtacctccat
     1441 aaaatggctt cttatctggc gttaaaaata catgaagtgg ccaacctccg cttcctgtaa
     1501 ttgcttgcac tgcatccata taaattgcat caatatctgg tctttcttct ctatctattt
     1561 taatgtttat aaaatatttg ttcattaaac ttgcagtttc tacatcttca aagctttctt
     1621 tctccattac atggcaccaa tgacaagctg cataaccaat gctaagtaat atgggcttat
     1681 tttcttcttt tgccaattta aaagcctctt cgccccaagt aaaccagtta acagggttgt
     1741 gggcatgttg tagaagataa acggaggttt cactaattaa tttattagtg tacataataa
     1801 tatactttaa ggaaataaag ttacctgttt gctaaaagaa taaaagcaat gattagtgtt
     1861 tctacaaaaa ataccttaag tagtaaagtg tattaatttt tatttttttc agctaaatat
     1921 ttatctaatg cagtaatcat actaggcact tgtggtattg gtgctttaat taaagtttta
     1981 agaccggcat tttcggcagc taataaagtg ttatttccaa aaaccccaat ttcagtattt
     2041 ttttgtttaa actcaggtat attgtcaaat aaacttttta taccacttgg tgtaaaaaag
     2101 caaattacat cataacttgt ttttttaaaa aactctttaa tatcgttgct aatggttcta
     2161 tacataaagg ctaaagtaaa atcacaatga tggtttttaa accagtttac aatctcatta
     2221 tcttgttgat tttcgctaca cacatacata aatctttcat tttctttatg tttgtttatc
     2281 acatcaaata aacttttatt ggatccgtct gcaccataaa aaacttttct ttttctatat
     2341 aaggtaaact tttgaagata taaagctaca gcctcagtaa tacagaaata tttagtatct
     2401 tgactaactg gtactttcat ttcttcgcac attttaaaaa agaagtcaat ggcatttcta
     2461 ctggtaaaaa ctacagcaga atacgaagca atatctattt tttgtttacg aaattcttta
     2521 gcagtaattg catctaattt aataaatgga gtaaaaaata attgcacatc gtattttctc
     2581 tccaaatcaa aataaggaga tttttcactt tcgggacgag gctgagaaat taaaattctc
     2641 tttactgctt ttgttggtac tgtacttttt gctcctattt tagccatgct tttctacaaa
     2701 atttatgcta taaattactc ttaaatacat taaagacagc cttatacaat aacaccatag
     2761 gcaatacttc aacggcacaa aggtaaagaa aaaaatgtaa ggcacttact ttaaaactat
     2821 ttcgtacaga ttttattcct aaccaatacc tatagatgaa aagtatagct attaatatta
     2881 aagcaatgat aaggcttacg taagcaatat tgtgcaaaga gaaagccatt attaaaataa
     2941 ctggaattag aattacgcct aataatttat taattataaa aacggtaaat acataggcat
     3001 taatggtttt ggcaatgtta aatacccatc caataaaact taaaaaaatg tatttaaaaa
     3061 agtaaacgga tgtaattaaa atggtgctat ataataatgt taaccaaaaa ttttctatca
     3121 gtaaatggta ttgctttgca attaaagtta taaaaatact actgcttata aaaaatagaa
     3181 tattcattaa tagagatggt aaactatctt gcagtaattg ctctctagtt tgtttttgcc
     3241 taaaagatga ttgaaaaaag attttaaata tatctgaaaa atatttcgag aatattgctt
     3301 taattaagga agcaaaaagt aaagtaccaa ttagtaaata aaataaaaag tggttatttt
     3361 ctgcttttct ttttacatca atcataaata ttctgctacc atttacaggg tagtacttag
     3421 aactaaaaag tgattcgaat gttgaagtgt ctatgagtaa tttttttggc tctgctattt
     3481 taattatagc agaatctggt gaattgcttt taacagaatc ttgagtagaa ataatagtat
     3541 ctgtaatatg taagatagaa tctgaaattg ttttaggctt aactaagggt ttactctttt
     3601 ttttagtggt atcttttatt tgggttttaa gagtatctat tttactagta tctacttgtg
     3661 caaatagaaa attgcaatta agtaaaagca caaaaatgta tatagcaatt ctcaagctta
     3721 gaaatttttg gcaaaattaa tagagaaggt tcctaaaaaa aacttacttg tttaaaaaag
     3781 caaagctttc ttaaacaaaa ttttaactaa atatgaaatt tgcttttaaa cctttaaacc
     3841 aattgcttgt ctatgattat acaaaccatt taaaaacaat aaaaaaataa acaatgaaac
     3901 gaattacaat tttatttatg agtgcaagtt tattgctaag ttttacagta acacaagcac
     3961 aagaaacagg tggtaaaaga agaggaaaat tggagaaaag agaaaaaatg gctggtgata
     4021 aaaaagctgg ctttggtaaa gatttaaact taacagagga acaaaaggcc gaagctaaaa
     4081 aaattaatga ggagtataat gcaaaagtta aagcactaaa aagcaatgat aaaataacca
     4141 tgggtgagta taataagcaa atggctgctt tgcaaaaaga aagaagagaa aaattagaag
     4201 ctattttaac tcctgaacaa aaagaaaaag tagaatcaag aaaacaagac aaggaagaca
     4261 aaatgcaaaa aagagaggaa atggtactag aaaaaatgaa agaaaattta aacttatccg
     4321 acgatcaggt tacaaaaata aaaaccatta atgaaactta tgccaaaaaa gcacaagctg
     4381 ttaggaatga caaaaattta tctcaagagc aaaaaaagca agcattatta gctattgcta
     4441 aagaaagaaa agaagcaata ggtaatactt taacaactga acaaaaagaa aagcaaaaaa
     4501 atttaagaaa cagagactga ggtgttagat ttagaagtaa aaaaacctta caaattgtaa
     4561 ggtttttttt atttatttgc tgcaaatata aaatttatta atggagcatt tattgactac
     4621 tgaaagttta attagttttt taatactggt ggtattagaa attgttttag gtattgataa
     4681 cgtaattttt gtaagtataa ttttaaacc
//
LOCUS       DAOQPK010000173         4685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_11539_length_4685_cov_3.719870, whole genome shotgun sequence.
ACCESSION   DAOQPK010000173 DAOQPK010000000
VERSION     DAOQPK010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11539_length_4685_cov_3.719870"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="PLU36_08260"
     CDS             <1..1028
                     /locus_tag="PLU36_08260"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HMZ46780.1"
                     /translation="ENLFLFNFSIFVIMQIFDNILETIGNTPIVKLNKITAHIPATIA
                     VKIESFNPGHSIKDRMALKMIEDAEKDGRLQAGGTIIECTSGNTGMGLAMAACIKGYK
                     CIFTTTDKQSKEKFDMLRAMGAEVIVCPTNVDADDERSYYSVAKKLSQEIPNSVWMNQ
                     YDNLSNRQAHYESTGPEIWQQTDGKITHFVVGIGTGGTVTGTSKYLKEQNPAIKTYGI
                     DTYGSILKQYKETGTFDPAEIHSYITEGIGEDIIPENYDFSLIDYIEKVTDKEAALMQ
                     RRLAKEEGIFAGNSAGAAIQGVLQIHEKFPFTKDDLVIIILHDHGSRYVGKFFNDDWM
                     KEKGFLD"
     gene            complement(1098..1622)
                     /locus_tag="PLU36_08265"
     CDS             complement(1098..1622)
                     /locus_tag="PLU36_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46781.1"
                     /translation="MVAIIAIMASCTKEGPQGPAGTNGVDGADGEDGNANVQVLLWNT
                     APTPTAGGTFHFRHGTITSQIIEEGAVLYYYLNGGNWVATPYFNSGATYGVRPAVSTG
                     VLTLTTFGGATFDISNTKSRIVLIGGSGKGTLVITRTKPWASLNEMEKAQSLYPNVDF
                     SDYNAVKKAFNLKD"
     gene            complement(1838..2389)
                     /locus_tag="PLU36_08270"
     CDS             complement(1838..2389)
                     /locus_tag="PLU36_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46782.1"
                     /translation="MEKLTKILMIAVIAIMASCSKEGPQGPAGTNGTNGVDGNANVKY
                     SDWFTPSPWVATTAYGTKDFAYTKTDAAITQSIIDNGVVLVFGKLAGYNPSIWPSTRV
                     SQLPIVVNYVSSGAQIDTWSAYLTLGTLTIDFKNNNNYYDNISITNQFRYVIIPSSSH
                     LRLSKPLQSMTYDEICSLYNIPK"
     gene            complement(2627..3154)
                     /locus_tag="PLU36_08275"
     CDS             complement(2627..3154)
                     /locus_tag="PLU36_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HMZ46783.1"
                     /translation="MKKLTKILMIAVIAIMASCTKEGPQGPAGTNGATGPVGPIGPEG
                     PIGNANVMYSDWFDAGAWTTSTTGSEKFYFDRPEAKITQAILDNGVVLAYTKLISDGS
                     NIRALPANTETSTFWNYILTVGNIRFTTNLASTPRPTNKFRYVIIPSTTHLRMAKPLT
                     QMSYDEVCELYNIPK"
     gene            3353..4099
                     /locus_tag="PLU36_08280"
     CDS             3353..4099
                     /locus_tag="PLU36_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HMZ46784.1"
                     /translation="MKIISLVPSITQLLFDVGLENSIVGRTKFCIHPTKKINTIPTVG
                     GTKNIDLQKINLLQPDIIFATKEENEKDQIVALKKDYKVIVFDIKNLEDNYKMIEKIG
                     KLTSTENKAQEIIKQTKQNFEVLKTTPSPSLSLSLYLIWRKPYMTIGKDTFIHAMLEE
                     IGLKNMFAHQTRYPIIQNLQTTYFEKCNLVLLSSEPYPFTEKHIKEIQDLLPNAKILL
                     VDGEYFSWYGSRIMETPIYFQQLLKKINNI"
     gene            4169..>4685
                     /locus_tag="PLU36_08285"
     CDS             4169..>4685
                     /locus_tag="PLU36_08285"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220699.1"
                     /note="catalyzes the formation of glycerone phosphate and
                     D-glyceraldehyde 3-phosphate from D-fructose
                     1,6-bisphosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HMZ46785.1"
                     /translation="MSKYTDLIGEERAKFLLEHQCKTIDQSLLHLPSADFVDKIFIQS
                     NRNPQVLRSLQQLFGNGRLANTGYVSILPVDQGIEHSAGASFAPNPIYFDSENIVKLA
                     IEGGCNAVASTYGVLGSVARKYAHKIPFIVKINHNEFLSYPNTFDQIMFGSIKDAWNM
                     GAVAVGATIYFG"
BASE COUNT         1645 a          714 c          723 g         1603 t
ORIGIN      
        1 tagaaaactt atttctattt aatttttcta tctttgtcat aatgcaaatt tttgataata
       61 tattagaaac tattggcaat acgccaattg taaaactgaa taaaataaca gcacatatac
      121 ctgcaactat tgctgtaaaa atagaatcgt ttaatcctgg ccattctatt aaagatagaa
      181 tggctttaaa aatgattgaa gatgctgaaa aagatggaag attacaagct ggtggcacca
      241 ttatagagtg tacttctggc aatacaggca tgggcttggc tatggcggct tgtataaaag
      301 gttataaatg tatttttact actaccgata aacaatccaa agaaaaattt gatatgctac
      361 gagcgatggg cgcagaagta attgtttgtc caactaatgt ggatgcagat gatgaacgtt
      421 cttattattc tgtagcaaaa aaattatcac aagaaattcc aaattctgtt tggatgaacc
      481 agtatgataa tttatcgaat agacaagcac attatgaaag tacaggacca gaaatttggc
      541 aacaaacgga cggtaaaatc acgcattttg tagttggtat tggcactggt ggcaccgtta
      601 ctggaacttc taaatattta aaagaacaaa atccagctat taagacttac ggaatagata
      661 cgtatggttc tatcttaaaa caatataaag agactggtac ttttgatcca gctgaaattc
      721 attcttacat tacagaagga attggcgaag atataattcc tgaaaactat gatttcagtt
      781 tgattgatta tatagaaaaa gtaacagaca aagaagctgc tttaatgcaa cgcagattag
      841 ccaaagaaga aggcattttt gcaggaaact ctgctggtgc tgctatacaa ggcgtgttac
      901 aaatacacga aaaatttcca tttactaaag atgatttagt aattattatt ttgcatgacc
      961 acggctcacg ttatgtagga aaattcttca atgatgattg gatgaaagag aaaggatttt
     1021 tagattaaaa attctattta ataaaacaca aaagctactc aattgagtag ctttttttat
     1081 aaatatttca caaagtttta atctttcaaa ttaaatgctt tttttactgc attgtaatca
     1141 gagaaatcaa cattaggata taggctctga gctttttcca tttcgtttaa cgaagcccat
     1201 ggtttagtgc gtgtaataac taatgtacct tttccacttc caccaattaa tactattctt
     1261 gatttagtat tcgaaatatc aaaggttgct ccaccaaatg tagttaatgt taatacacca
     1321 gttgaaactg ctggacgcac accataagta gctccactat taaaataagg cgttgccacc
     1381 caatttccac cattcaaata ataatacagt acagcacctt cttcaattat ttgacttgta
     1441 attgttccat gtctaaaatg aaaagtacca cctgctgtag gtgttggagc agtattccat
     1501 aataaaactt gaacattagc attgccatct tcgccatctg caccatctac gccattggta
     1561 cctgctggtc cttgtggacc ttctttagta cacgacgcca ttatggcaat tattgcaacc
     1621 attaaaattt tttagagatt cttcatgagt tttaatttaa atttattaat aatagataaa
     1681 tgtaattcta ttcattattt tctttaaaag aattgagtaa ttggtagaaa ttatttatta
     1741 gttggtaaaa aaagagcatt tagattaatt atgccacccc aataaaacac aaaagctact
     1801 caattgagta gcttttgtta tagaaataat taaatgctta ttttggaata ttgtacaatg
     1861 aacatatttc gtcataagtc atgctttgta aaggtttgct taaacgtaaa tgtgaacttg
     1921 atggaataat tacatatcta aactggtttg taattgagat attatcataa taattattat
     1981 tgtttttaaa atctattgta agagttccta gtgtcagata tgcagaccat gtatctattt
     2041 gtgcaccact gcttacataa tttactacaa taggtaattg tgacactcga gtgctaggcc
     2101 aaatgcttgg attataacca gctaattttc cgaaaactaa tactacacca ttgtctatta
     2161 tgctttgcgt aattgctgca tcagttttag tatatgcaaa atcttttgta ccataagcag
     2221 tagtagctac ccatggactt ggtgtaaacc aatctgaata ttttacatta gcgtttccat
     2281 ctacgccatt tgttccgttt gttcctgctg gaccttgtgg gccttctttg ctacatgatg
     2341 ccataatagc aattacagca atcattaaaa tttttgtaag tttttccata agttttgttt
     2401 tattttattt ttaataatag ttaaatgtaa atcattcatc tatacttgtc aattttaatg
     2461 agtaagtggc tcgtattaat tagtattcgg taaaaaatag gtatttttca tttttaaaaa
     2521 ttataagtaa atgaaagaaa aaggattttt agattaaata ttatacttta ataaacacaa
     2581 tagctactca attgaatagc ttttttaatt gaaaatagtt taatacttat tttggaatat
     2641 tatacaattc acatacttca tcgtaagaca tttgtgttaa tggttttgcc atacgaagat
     2701 gagtagtaga tggaatgata acataacgaa acttattagt gggtctaggt gttgatgcaa
     2761 gatttgtagt aaatctaata ttacctacag ttaagatata attccaaaat gtagatgttt
     2821 ctgtattagc aggtaaagct cttatattac taccatctga tatcaatttt gtatatgcca
     2881 aaacaacacc attatctaat atggcttgag taattttagc ttcaggtctg tcaaaataga
     2941 atttttcact acctgtcgtt gatgtagtcc aagcgccagc atcaaaccaa tcggaataca
     3001 ttacattcgc attacctatt ggaccttctg gtccaatagg tccaactggt ccagtcgcac
     3061 cgtttgtacc agctgggcct tgtggtcctt cttttgtgca tgaagccata atagcaatta
     3121 cagcaatcat taaaattttt gtaagttttt tcataagttt tgtttttatt tttaataata
     3181 aattttgtat tgcaaagatt attgtatttg aaatctaaaa caattggaat tttagcattt
     3241 tgtgagagta atttattaaa aaaacaaaat atctaattaa caatcaatta cttaatttat
     3301 ttatcagata ttaaaaaatg ttgtaatttt ccaaaaaata aattatcttt taatgaaaat
     3361 catttcttta gttccatcta ttacacaact actttttgat gtaggattgg aaaacagtat
     3421 tgttggcaga actaaatttt gtattcatcc aacaaaaaaa ataaatacta taccaactgt
     3481 tggtggtact aaaaatattg acttacaaaa aataaactta ttacaacctg atatcatttt
     3541 cgctaccaaa gaagaaaatg aaaaagatca aatagtagca ttaaaaaaag actataaagt
     3601 aattgtattt gatataaaaa acttggaaga caattacaaa atgatagaaa agattggtaa
     3661 acttacttct actgaaaata aagcgcagga aattatcaaa caaactaaac aaaattttga
     3721 ggtattaaaa acaacacctt caccttcact atcactttca ctttatttaa tttggcgaaa
     3781 gccttacatg acaattggta aagacacttt tattcatgct atgttagaag aaattggttt
     3841 aaaaaatatg tttgcacatc aaacacgtta tcctattatt caaaatttac aaactactta
     3901 ttttgaaaaa tgtaatttgg ttttattgtc atcagaaccg tatccattta cagaaaaaca
     3961 cattaaggaa atacaagatt tattgccaaa tgcaaaaatt ctattagtag atggcgaata
     4021 tttctcttgg tatggcagca gaataatgga aactcctatc tactttcaac aattacttaa
     4081 aaaaatcaac aatatttaaa aaaacttacc acttacacct tatgacttac gactatttgc
     4141 tatctttgca cacgtaaaaa aagtagcaat gagcaaatac acagacctaa taggcgaaga
     4201 aagagcaaaa ttcttattgg aacatcaatg taaaactatt gaccaatctt tattgcactt
     4261 gccaagtgct gattttgtag ataagatatt tattcaaagc aatcgtaatc ctcaagtatt
     4321 acgtagttta caacaattat ttggcaatgg tcgcttagcg aatactggtt atgtgtctat
     4381 tttgcccgtt gaccaaggta ttgaacattc tgctggtgct agttttgcac caaatccaat
     4441 ttattttgat tctgaaaaca ttgtgaagtt ggcgatagaa ggtggttgca atgcagtagc
     4501 ttctacttat ggcgtgttag gttctgttgc cagaaaatat gcacacaaaa ttccatttat
     4561 agtaaaaatt aatcacaacg aatttttatc gtatccaaat acatttgacc aaattatgtt
     4621 tggttcaata aaagatgctt ggaatatggg tgctgttgct gtaggtgcta ctatttattt
     4681 tggtt
//
LOCUS       DAOQPK010000174         4676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_11577_length_4676_cov_5.444276, whole genome shotgun sequence.
ACCESSION   DAOQPK010000174 DAOQPK010000000
VERSION     DAOQPK010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11577_length_4676_cov_5.444276"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1557
                     /locus_tag="PLU36_08290"
     CDS             <1..1557
                     /locus_tag="PLU36_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HMZ46786.1"
                     /translation="TLAVKRAKANIKTLLDQRPDEVTILENNQAKTIKAETVQIGNII
                     QLKAGEKLGLDGELLTDNASFNTAALTGESKPDTKAKGETVLAGMINLNTVAQVKVTT
                     AYTDSKLSKILELVQNATAQKAPTELFIRKFAKIYTPIVVLLALLITVLPYFFVENYI
                     FSQWLYRALVFLVISCPCALVISIPLGYFGGIGAASKNGILFKGSNFLDIIANIQNVV
                     MDKTGTMTEGVFKVQEEILKPEFNKTEILQLVNALESQSTHPVATAIHNYVGNVDSNI
                     KLENVEEISGHGLKATINNKEILVGNLKLMDKFNIIYDIDPTTIVYTLIAIAYDKKFV
                     GYLTIADSIKEDAQITIDKLKALNVKTTMLSGDKSTVVKFVADKLSITNSFGDLLPED
                     KVNKVKEIKANNETVCFVGDGVNDAPVVALSDVGIAMGGLGSDATIETADVVIQDDMP
                     SKIPMAINIGKQTKKIVWQNIVLAFAVKAIVLILGAGGLATMWEAVFADVGVALLAIL
                     NAVRIQRMRF"
     gene            complement(1672..1851)
                     /locus_tag="PLU36_08295"
     CDS             complement(1672..1851)
                     /locus_tag="PLU36_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46787.1"
                     /translation="MYYKATLCLSSFRQFLYWHKRSGGGGWNEAFFCCYALYWLGSLA
                     VERSGTGNVPSAVVA"
     gene            2652..3080
                     /locus_tag="PLU36_08300"
     CDS             2652..3080
                     /locus_tag="PLU36_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46788.1"
                     /translation="MRTIKIYTFNPKNPVNEFDIYVLCKGLNSGKPLEKPCPNCFVLA
                     CKNQAEKDFYTTLLFGLWKVKHFYQFHTGSVIPFIRISDFKQVVKEQANRVSIKPQDF
                     IEDVHKFRLIERKQKQIYEQMALINDVKRALIYRHFHSKR"
     gene            3321..3554
                     /locus_tag="PLU36_08305"
     CDS             3321..3554
                     /locus_tag="PLU36_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46789.1"
                     /translation="MSVQHISQSATSSAVCSCGSCQLGSLVAVSPFAHQRRVRRRFFS
                     WSPSVGCRVYSLACGCFLWVYASGQRFVSSLPF"
     gene            complement(3949..>4676)
                     /locus_tag="PLU36_08310"
     CDS             complement(3949..>4676)
                     /locus_tag="PLU36_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46790.1"
                     /translation="GDFAGLVGGPTAEQIYNDSAFERTRENMSEFGGCASAGKAVRVG
                     LSQIIKQMSDSRLTGRLTALMKQINLEDTANPRGQRSIEISAFQNLFNGFNFDRNVSL
                     NGIFNAPYTLTNATTRDSADFVIPAFNPANLVNAPAGATHFRLVNAIVTISDWVYNPA
                     TGSYEPTDPTLNSLSDISYSAYLPLNATAPATTVTTTLPGAPTMTATVSVLNCVGIEF
                     YQQVGGNYYLFAQGNALRCDTVF"
BASE COUNT         1603 a          904 c          791 g         1378 t
ORIGIN      
        1 acattagccg taaaaagagc caaagccaat atcaaaacct tgcttgacca acgacctgat
       61 gaagtaacca ttttagaaaa caaccaagcc aaaactatta aagcagaaac ggtacaaata
      121 ggtaatatta ttcaacttaa agcaggagaa aaattgggat tagatggaga attattaacc
      181 gacaatgctt cctttaacac agcagcactt acaggcgaaa gcaaaccaga caccaaagca
      241 aaaggcgaaa ccgtgttagc aggaatgata aacctaaaca ccgtagcaca agtaaaagtt
      301 actactgctt ataccgatag taagttaagt aaaattttag aattagtaca gaacgccaca
      361 gcacaaaaag caccaaccga attatttatt agaaaattcg caaaaatata tacaccaata
      421 gttgtacttc ttgctttact cattacagta ctaccttact tctttgtaga aaattatatt
      481 tttagtcaat ggctttacag agctttagtt ttcttggtta tctcttgtcc ttgtgcattg
      541 gttatcagta ttccattagg ttattttggt ggcataggtg cagcttctaa aaatggaata
      601 ttatttaaag gcagtaattt tttagacatt attgctaata ttcaaaatgt ggtaatggac
      661 aaaacaggta caatgactga aggtgttttt aaagtgcaag aagaaatatt aaaacccgaa
      721 tttaataaaa cggaaatact acaattggtc aatgcccttg aaagccaaag tacccaccca
      781 gttgctactg ctattcataa ctatgtagga aatgttgata gcaatattaa attagaaaat
      841 gtagaagaaa tatcaggtca cggactaaaa gcaacaatca ataacaaaga aatattagta
      901 ggtaacttaa agctaatgga taaattcaat attatttatg atattgaccc aacaaccata
      961 gtttatactt taatagccat tgcttacgat aaaaaatttg taggatattt aaccattgca
     1021 gatagtatca aagaagatgc acaaattaca atagataaac tcaaagcatt gaatgtaaaa
     1081 accacgatgt taagtggcga taaaagtacc gttgtaaaat ttgttgccga taaattaagt
     1141 attaccaatt cctttggcga tttacttcca gaagacaaag taaataaagt aaaagaaatc
     1201 aaagccaata atgaaacagt atgttttgta ggcgatggtg taaacgatgc accagtagta
     1261 gcattaagcg atgttggtat agcaatgggg ggcttaggca gtgatgctac aattgaaact
     1321 gccgatgtgg tcatacaaga cgatatgcca agcaaaatac ctatggcaat aaacataggt
     1381 aaacaaacaa aaaaaatagt ttggcaaaat attgttcttg catttgcagt aaaagcaatt
     1441 gtgctaatac ttggtgcagg tggtttagcc acaatgtggg aagcagtatt tgcagatgta
     1501 ggcgtggcat tattagcaat attaaatgca gtgagaatac aaagaatgag gttttagccc
     1561 ccaagccaac acacaaaaaa atacggctta gggctttgcc agcacacagt gccaacacac
     1621 aaagccctct aaggtattgc agtcagtttt taactgtcat attttttgtg gctatgctac
     1681 cacagcactt ggcacattgc ctgttccact tcgttccaca gccaaagagc caagccaata
     1741 caaagcatag caacaaaaaa acgcttcgtt ccaaccacca ccaccacttc gcttatgcca
     1801 gtataaaaac tgcctgaagc tacttaaaca taatgttgcc ttatagtaca ttttacccac
     1861 gcttataggc gactatgcaa atgcaaaaaa tgtcctataa gaacattatg ttaagtatcc
     1921 aataccttag cacccttgca ttaaaaaggg tagtagttta ttttcaatgg tatttttaat
     1981 gcaagtgtga agccgtattt tttaccaacg ctacaccccc cagagccaca caccccacca
     2041 cccatttcag cggtgcagaa aaaaataaag cattgttatt tactgccaat gtgcttaata
     2101 aagtgttcat tcattccgtt acattccatt acggctggct atcgctaata tgccttcact
     2161 tcattacact acattcatat cacaccttat tttaagcacc gcacagcaag agtatttcgt
     2221 acctcataca cttgctttta acctacgcca ctacttttgg tatgctttat tttttctgcg
     2281 tattgtttgc cccattccta cgctacaaaa ggatttaatt ttttgatatt taaaaataag
     2341 aatgatgcaa gaagttttcc aaagagaaaa aacaaaccga ataaaaagga aagcaaagtt
     2401 aggtggcttg cacaagccta cacactaaaa aatccaaaag actacggcat aatgaaaggc
     2461 aaataatcta agtttttttt attggtattt gcctttcagc cttcggcact tattccgttt
     2521 ccttttggct tttttgcccc gccacctttt tagatgcttt cttttttttc tgtttttttc
     2581 tttgtaaaaa tatttgttgg tttcagccac agcaaggaac aaagcaggta acttttaaag
     2641 caatgtttct tatgcgaact attaaaattt acacattcaa tccgaaaaac cctgtaaatg
     2701 aatttgacat ttacgtacta tgtaagggct taaatagtgg caaaccactt gaaaagcctt
     2761 gccctaattg ttttgtctta gcttgtaaaa atcaagcaga aaaagacttt tacactacct
     2821 tacttttcgg cttatggaaa gtaaagcatt tctaccaatt tcatacaggg tcagtaattc
     2881 catttataag aatatccgat tttaagcaag tagtaaaaga acaagccaac cgagtaagca
     2941 taaagcctca agatttcatt gaagatgtac acaaattcag gctcatagaa cgaaagcaaa
     3001 aacagattta cgaacaaatg gcattaatca acgatgtcaa aagagcctta atatacaggc
     3061 atttccacag caaacggtaa aagtaggctg aatatgccgt caactgccat aaataaaaat
     3121 caaatatgca aaagtagcaa gggtggcaac gcacaaaccc aaccaaaaaa aaacaaaaga
     3181 ctacggcata atgcaaaaaa aataggtgca aggcaaagcc aaatatgcac ctattttttt
     3241 gcacccttcg ggacttattc cgtttccttt tgtttttttt atcacccttg ctatgaaaag
     3301 catattaatt tttatttatt atgtcagttc agcacatttc tcagtcagcc actagttcag
     3361 ccgtttgcag ttgtggttct tgtcagttag gctctttagt agccgtttcg ccttttgccc
     3421 accagcgtag ggttcgtagg cgttttttct cttggtcgcc ttctgtgggt tgccgtgttt
     3481 attctttggc ttgtggttgc ttcctttggg tgtatgcttc gggtcagcgt ttcgtttcgt
     3541 ctttgccctt ttagggcatt gatgaaactt tgaaaccaac catacagacc gaaccaccca
     3601 aactaaaatc atatccaccc gataaagtcc aacaagtcta accagtctaa ctacttcttg
     3661 acttgaccac ttttggactt tagcccaagt ataacacttg cctatactta tattaactat
     3721 actacttaga ctatttagac tacttagacc actatattaa aattgactta attgctattt
     3781 gaaagcaatt taaagcacat ttaagcccta tatatttaac ccacctacac aaatttgtga
     3841 aatagaatgc aataggtttc aaagcaatat tttttattga atagggtaag ccataaaaca
     3901 aaaaacccca ccaacaaggg cggggcttta agaagcagta gaaagatatt aaaatactgt
     3961 atcgcaacga agtgcattgc cttgagcaaa taggtaataa ttacccccaa cttgttggta
     4021 aaactcgata cctacacagt ttaaaacgct aacagtagca gtcattgtag gagcaccagg
     4081 aagagtagtt gttacagttg ttgcaggagc tgtagcattc aatggtaaat atgctgaata
     4141 gcttatatca cttaatgaat ttagagtcgg atctgttggc tcataacttc ctgttgcagg
     4201 attataaacc caatctgaaa tcgttacaat tgcatttact aatctgaaat gagtagcacc
     4261 agcaggagca tttacaagat ttgcaggatt aaatgcagga attacaaaat ctgcactatc
     4321 tctggttgta gcatttgtaa gagtataagg tgcgttaaaa ataccgttta aacttacatt
     4381 tctatcaaaa ttaaatccat tgaataaatt ttgaaatgcc gaaatttcga tacttctttg
     4441 acctcttggg tttgcagtat cttccaaatt gatttgtttc attaaagctg ttaagcgacc
     4501 agtcaaacga ctatcactca tttgctttat gatttgagat aagcctacac gaacagcttt
     4561 accagcagaa gcacaaccac caaactcaga catattttct cgagttcttt caaatgcact
     4621 atcgttataa atttgttcgg cagtaggacc gcctacaagt cctgcgaagt cgcctt
//
LOCUS       DAOQPK010000175         4671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_11598_length_4671_cov_3.556326, whole genome shotgun sequence.
ACCESSION   DAOQPK010000175 DAOQPK010000000
VERSION     DAOQPK010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11598_length_4671_cov_3.556326"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1513)
                     /locus_tag="PLU36_08315"
     CDS             complement(<1..1513)
                     /locus_tag="PLU36_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HMZ46791.1"
                     /translation="MKFEKEINRRKTFAIISHPDAGKTTLTEKLLLFGGAIQTAGAVK
                     SNKIKKHATSDFMEIERQRGISVATSVMTFEYKEHLINLLDTPGHKDFAEDTYRTLTA
                     VDSVILVIDSVNGVEDQTRRLMEVCRMRDTPVIVFINKMDRDGKNRFDLLEEIEKELK
                     ITLHPMTWPINSGKDFKGVYNIHHKNLRLFSANTKASEEDTIAIENLNEEILDKKIGE
                     RDAIQLREDAELIEGVYGILNEKEYLCGKIAPVFFGSAVNNFGVKEMLETFITIAPTP
                     LCRQTNTREVCPGEEKFSGFIFKIHANLDPKHRDRIAFMRVCSGKFERNKYYNHVRLE
                     KTMRFSNPYSFLARSKDTIEDAYPGDVIGLFDTGNFKIGDTLTEGEKFYYTGIPTFSP
                     EIFKELVNKDPMKTKQLEKGIQQLTDEGVAQLFTQFGGNKKIIGCVGELQFEVIQYRL
                     LQEYGAACEFRVLPFYKACWLTSKDKQKLEDFLRFKQQNSGEDKDGNPIYLAQS"
     gene            1588..2178
                     /gene="coaE"
                     /locus_tag="PLU36_08320"
     CDS             1588..2178
                     /gene="coaE"
                     /locus_tag="PLU36_08320"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793821.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HMZ46792.1"
                     /translation="MIKVGITGGIGSGKSIVSNIFKILNIPVFDADFEAKKIMTESVV
                     VKQQLIATFGNAVFLNNELNRQYLSTIVFSDAHLLEKLNAIVHPAIIDAGNEWANQQI
                     SPYVIKEAALLFEAGSASDLDYVIGVYAPKIIRLQRVMQRDKVDKQAVLNRMSKQIDE
                     EIKMKLCDFIITNDDEQMVIPQVLKLHEKFLAMSNM"
     gene            complement(2180..3580)
                     /locus_tag="PLU36_08325"
     CDS             complement(2180..3580)
                     /locus_tag="PLU36_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HMZ46793.1"
                     /translation="MALSKIWSAFIITAIIVALCRMLFFDGGTNLFSKMVVGKHGDTT
                     NTKTIDSNHLSQTILATIKENKEFKNGETKYTKNKEGNYIVYNEQSADGILATCTVAV
                     NICIGLIGIMALFMGFLAIAEKAGAINFLSRIIGPFFNKLFPDLPKNHPAFGHMILNF
                     SANLLNMDNAATPFGIKAMQSLQEANPDKDKASNAQVMFLCLHASGLTLIPITIIAFR
                     AQLGAANPTDIFIPCMIATFAATMAALLIVSFKQKINVLQPIILMWVLGISAIIAALV
                     LYIKSLSKNEVDVFSGLLSNGLILLIFLLIVLGGLYKKVNVFDAFVEGAKGGFETAIK
                     IIPYLVGMLVAISLLRTSGTFDALINGMKHLFLAIGMDTRFVDGLPTALIKPMSGSAA
                     RGMMIDTMRIFGADSYAGRLSSILQGSSDTTFYVVAVYFGAVGIKNTRYTIGSMLLAD
                     LVGIITAIILSYMFFT"
     gene            complement(3659..4375)
                     /locus_tag="PLU36_08330"
     CDS             complement(3659..4375)
                     /locus_tag="PLU36_08330"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HMZ46794.1"
                     /translation="MTKLSVNINKFATLRNSRGGNNPNVVKAAINAQQFGADGVTVHP
                     RPDERHITYNDVREIKKIIITEFNIEGNPREDKFVKLVLETLPQQVTLVPDALGQLTS
                     DHGWNTIEHKNYLIDIIAIFKNAGIRVSIFVDPIEEMIAAAALTNTDRIELYTESYAK
                     QFSINKENAIAPYIKAANKAFDLGLGINAGHDLDLTNLNYFAKNIPQLNEVSIGHALI
                     SDALYMGFENTIQIYKRQLL"
     gene            4472..>4671
                     /locus_tag="PLU36_08335"
     CDS             4472..>4671
                     /locus_tag="PLU36_08335"
                     /inference="COORDINATES: protein motif:HMM:NF040120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HMZ46795.1"
                     /translation="MLLQKSKIDFGLLLLLAFNIYAIYYYYQNPNALGALIVIFWLQS
                     VFIGIFNVIGMLTFTNRVENSFT"
BASE COUNT         1483 a          756 c          696 g         1736 t
ORIGIN      
        1 cgctttgggc taaataaata ggattaccat ctttatcttc tccgctattt tgttgtttaa
       61 accttaaaaa gtcttcaagt ttttgcttgt ctttgcttgt aagccaacaa gctttgtaaa
      121 atggtagtac tctaaattcg caggctgcac catactcttg taatagcctg tattgaatta
      181 cttcaaactg taattctcct acacacccta taattttttt attaccgcca aactgtgtaa
      241 ataattgtgc tacaccttcg tcggttaatt gttgaattcc tttttcgagt tgtttggttt
      301 tcatggggtc tttattaacc aattctttaa atatttcagg agaaaatgta ggaatgccag
      361 tgtagtaaaa tttttctcct tcggttaaag tatctccaat tttaaaatta cctgtatcaa
      421 ataaacctat tacatcgcca gggtaggcat cttctatagt gtctttactt cttgctaaaa
      481 aagagtaggg gttgctaaaa cgcatggttt tctctaacct tacatggttg tagtatttgt
      541 ttctttcaaa cttgccactg caaactctca taaatgcaat tctatctctg tgtttagggt
      601 ctaagttggc atgaatttta aaaataaatc cactaaactt ttcttcacca ggacaaactt
      661 ctctagtgtt tgtttgtctg caaagtggtg taggggcaat ggtaataaag gtttcaagca
      721 tttcttttac accaaaatta ttaacagcac tgccaaaaaa tactggtgca atttttccgc
      781 ataaatattc tttttcattt aatatgccat acacgccttc tattaattct gcatcttctc
      841 ttagttgtat agcatctctt tcgccaattt ttttatctaa aatttcttca tttaaatttt
      901 caatagcaat ggtatcttct tcactggctt ttgtattggc agaaaataat cttaaatttt
      961 tgtgatgaat attatataca cctttaaaat ctttgccact atttattggc catgtcattg
     1021 gatgcaaagt aatttttaat tctttttcaa tttcttctaa aaggtcaaac ctgtttttac
     1081 catctctatc cattttattt ataaaaacaa ttacaggggt atctctcatt ctacatactt
     1141 ccattagtct tcttgtttgg tcttctacac cattcacgct atcaataaca agtattacac
     1201 tatctactgc tgttaatgtt ctataagtat cttctgcaaa gtctttgtga ccaggggtat
     1261 ctaataagtt tattaagtgt tctttatatt caaaagtcat tacacttgtt gcaacagaaa
     1321 ttccacgttg gcgttcaatt tccataaaat cgctggtagc atgtttttta attttattac
     1381 tttttacagc acctgcggtt tgtattgctc caccaaataa taaaagtttt tcggttaagg
     1441 ttgttttacc agcatctggg tgagaaataa tggcaaaagt ttttctacga tttatttctt
     1501 tttcaaattt cataaggcgg caaaggtaat tgctaaagtt ttagcttgtt caaactttag
     1561 tttgcttatt tttattcttt aaattgtatg ataaaggtag gtattacagg aggcattggt
     1621 agtggaaaaa gtattgtaag taatattttt aaaatattaa atattccggt atttgatgct
     1681 gattttgagg ctaaaaaaat aatgactgaa agtgttgttg taaaacaaca acttattgcc
     1741 acatttggta atgcggtttt tttaaataat gaattgaata gacaatactt gtcaactatt
     1801 gttttttctg atgctcattt gttagaaaaa ttaaatgcta ttgttcatcc tgccatcatt
     1861 gatgctggaa atgagtgggc taatcaacaa atttcgcctt atgttattaa agaagctgct
     1921 ttgttatttg aagcaggtag tgcatcggat ttagattatg taattggagt ttatgcaccc
     1981 aaaataatac gattgcaaag agtaatgcaa agagataagg tagataagca agctgtttta
     2041 aatagaatga gtaaacaaat agatgaggaa attaaaatga aactatgcga ttttattata
     2101 acgaatgatg acgaacaaat ggttatacca caagtattaa aattgcatga aaaattttta
     2161 gctatgagta atatgtagct taagtaaaaa acatatagct taaaataatt gcagtaataa
     2221 ttccaactaa atctgccaat aacattgagc caattgtata acgtgtattt ttaataccta
     2281 cagcaccaaa atatacagcc actacataaa aagtagtatc gctagagcct tgtaaaatac
     2341 tgcttaatct tcctgcgtaa gaatcagctc caaaaattct catagtatca atcatcattc
     2401 ctcttgcagc actgccactc atgggtttta ttaatgctgt tggtaaacca tctacaaaac
     2461 gagtatccat acctatagct aaaaacaaat gtttcattcc gtttattaat gcatcaaaag
     2521 tgccgctagt acgtagtaag ctaattgcaa ctagcatacc cactaaatat ggaataattt
     2581 ttattgctgt ttcaaaacct ccttttgctc cctctacaaa tgcatcaaaa acatttactt
     2641 ttttgtataa accacctaat actattaata gaaaaattaa aagtattaaa ccattactta
     2701 acaaacctga aaaaacatct acctcatttt tacttaaaga ttttatatat aaaaccaatg
     2761 ctgcaattat tgcactaata cctaataccc acattaaaat tatgggctgt aatacattaa
     2821 ttttttgttt aaaagataca atcaataatg ctgccatggt agcagcaaaa gttgcaatca
     2881 tacaaggtat aaaaatatct gttgggttgg cagcacctaa ttgagctcta aaagcaataa
     2941 ttgttatagg tattaaagtt aatccacttg catgaaggca taaaaacata acctgtgcgt
     3001 tagatgcttt atctttatcg ggatttgctt cttgtaagct ttgcatggct ttaatgccaa
     3061 aaggtgtggc tgcattatcc atgtttaaaa ggttagcaga aaagtttaaa atcatgtgcc
     3121 caaatgcagg gtggttttta ggtaaatcgg gaaatagttt attaaaaaat ggaccaataa
     3181 ttcgagataa aaaattaatt gcacctgctt tttctgcaat agccaaaaag cccataaaca
     3241 aagccattat tcctattaac ccaatacaaa tatttactgc aacagtacaa gttgctaaaa
     3301 taccatcagc actttgttca ttatacacaa tataattgcc ttctttgttt ttagtgtatt
     3361 ttgtttcgcc atttttgaac tctttatttt ctttgatagt tgcaagtatg gtctgtgata
     3421 aatggttgct atcaatagtt ttagtgttgg ttgtatcgcc atgtttgcct acaaccattt
     3481 tactaaataa attggttcct ccgtcaaaaa ataacattct gcataaggct acaataattg
     3541 cagttataat aaatgcactc caaattttac ttagtgccat atttttattt tatgagaaca
     3601 aaataagcaa gaaaatttac ttatgtaaat gaaaacaaaa ctaaatacta acatttggtt
     3661 acaatagctg cctcttataa atttgaatgg tattttcaaa gcccatatac aaggcatcgc
     3721 ttattaatgc atggccaatg cttacttcat taagttgtgg gatatttttt gcaaaatagt
     3781 taaggttggt aaggtctaaa tcgtgtcctg cattgattcc tagccctaaa tcaaaagctt
     3841 tattagctgc tttaatataa ggtgctattg cattttcttt gtttattgag aattgttttg
     3901 catatgattc tgtgtaaagt tcaattctat ctgtattagt taaggctgct gctgctatca
     3961 tttcttctat tgggtccaca aaaatagata cacgtattcc ggcattttta aatattgcaa
     4021 taatatctat taagtagttt ttatgttcaa ttgtattcca accgtggtcg gaggtaagtt
     4081 gtcctaaagc atctggtaca agtgtaacct gttgtggtaa tgtttctagc actaacttaa
     4141 caaatttatc ttctcttggg ttgccttcta tattaaattc tgtgataatt atttttttta
     4201 tttctcttac atcattataa gttatatgcc tttcgtctgg tcttggatgc acagttacac
     4261 catctgcacc aaattgctgg gcatttattg cagccttaac cacatttggg ttattgcctc
     4321 cacgactgtt tcttaaagtt gcaaatttgt ttatattcac actaagttta gtcattttgt
     4381 aattttatat atttataaaa aaacaaattt acaaaagcaa atggtaatgt ttttttgaat
     4441 ttttttataa cctttaagta aaattttttt tatgctccta caaaaatcaa aaattgattt
     4501 tggactttta ttgttattag catttaacat ttatgccatt tattattatt atcaaaaccc
     4561 aaatgcatta ggagcattga tagtaatttt ttggttacag agtgttttta ttggcatttt
     4621 taatgtaatt ggtatgctta cttttaccaa tagagttgaa aactctttta c
//
LOCUS       DAOQPK010000176         4644 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_11728_length_4644_cov_3.687514, whole genome shotgun sequence.
ACCESSION   DAOQPK010000176 DAOQPK010000000
VERSION     DAOQPK010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11728_length_4644_cov_3.687514"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1276)
                     /locus_tag="PLU36_08340"
     CDS             complement(<1..1276)
                     /locus_tag="PLU36_08340"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ46796.1"
                     /translation="MLCIFTICAAAVSAQQTIISGKVSNSKSKESVSAVSVTVKGTTS
                     GTYTDDKGEFKLVTTQKLPFTLVFSSVGFADKEVSITDASQSVQVSLDVKYSMGDEIV
                     VAASRVSEKIIESPVSIERMGASAVKNAVAPNFYDAILNFKGVDMTTSSINFRTISTR
                     GFNGSGNLRANQLVDGMDNQAPALNFSVGNIVGMTELDVDNVELLPGASSALYGSGGM
                     NGTILMSSKNPFKYQGLSFQVKQGINHVDSKQTTAAPYFDWAFRWGKKVTERFAFKIS
                     GQFIQAQDWQAVDYANLARNNVYSQITNGDRITDPNYDGVNVYGDEVGANMQYLARLG
                     LLASGIAPNSAAYTGLNSVAGMGLNYAQSVAFIQNNVPALVPALAAFPTVFSINRGYY
                     ASNISRTGYNEKDLVDYNSYNVKLSGGLYYKLK"
     gene            1434..2363
                     /locus_tag="PLU36_08345"
     CDS             1434..2363
                     /locus_tag="PLU36_08345"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HMZ46797.1"
                     /translation="MSAFQFPNQTNFYQGKVRDVYTINNHFVVMIASNRISAFDVILP
                     KPIPFKGQVLNQIAATMLQATKDICPNWLINSPAPNVSIGKKCEPFKIEIVVRGNLTG
                     HAWRTYKSGQRILCGIKLPNGLRENDFFENPIITPTTKASVGHDEDISKDEIITQNLA
                     SLNEWETLEKYALQLFERGKQIAAKQGLILVDTKYEFGKINGEIFLMDEIHTPDSSRY
                     FYAEGFEERIENGQSQKQLSKEFVREWLLANNFMGKEGQTVPTMSNEWVQTISLRYIE
                     LYEKILGEKFKPQELSNEETYHKIEQSLNQLIN"
     gene            2373..3266
                     /gene="fabD"
                     /locus_tag="PLU36_08350"
     CDS             2373..3266
                     /gene="fabD"
                     /locus_tag="PLU36_08350"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HMZ46798.1"
                     /translation="MNKHAFIFPGQGSQFKGMGKNLYETNSLAKEMFEQANEILKFRI
                     SDIMFEGSDEALKQTNVTQPAVFLHSVIAYSTLHNAKPDMVAGHSLGEFSALVANKTL
                     SFEDALLLVSIRANAMQKACESNPSTMAAVLALADDKVEEICSLVQKQSGEVVVAANY
                     NCPGQLVISGSIKGIEIACEQMKAAGAKRALVLPVGGAFHSPLMSEAKAELAMAIEAT
                     IFNTPICPIYQNVVAKGISDATQIKQNLILQLTGAVKWTQSVQAMVADGATHFTEAGP
                     GKVLQGLVQKIYKEAMVDGVS"
     gene            3291..3545
                     /locus_tag="PLU36_08355"
     CDS             3291..3545
                     /locus_tag="PLU36_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46799.1"
                     /translation="MLLKIILWGIVIYYLYKFVFGVVMPVSKATSDMKSKINEMQRAQ
                     EQFIKQQQQQQEQFQKQKVHEENIHLKKGDYIEFEEIKDK"
     gene            3556..3945
                     /locus_tag="PLU36_08360"
     CDS             3556..3945
                     /locus_tag="PLU36_08360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HMZ46800.1"
                     /translation="MSIFSKIIAGKIPCFKIAEDENFLAFLDIFPVQYGHTLIIPKIE
                     IDKLFDLPNEYLSTILVFAKPVAQAIQKAYPCNRVNIITVGLEVPHAHIHLIATNGLK
                     DVDLNKPKLKLTNEQLKEVQMNILKFL"
     gene            4402..4542
                     /locus_tag="PLU36_08365"
     CDS             4402..4542
                     /locus_tag="PLU36_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46801.1"
                     /translation="MEYRKTATKTKGKSVAKKTSTKQKGNVEAVLKAAKTNKLDLSYL
                     RE"
BASE COUNT         1730 a          703 c          757 g         1454 t
ORIGIN      
        1 ttttaagttt gtaatataaa cctccggaaa gttttacatt gtaagaattg taatcaacta
       61 aatctttttc gttatatcct gtacggctaa tattagaagc ataatatcct ctattgatag
      121 aaaaaacagt tgggaatgca gccaaagctg gtactaaagc tggtacatta ttttgaataa
      181 atgcaacaga ttgagcataa tttaatccca tacctgcaac tgagtttaag ccagtatatg
      241 cagcagaatt tggtgcaata ccacttgcta ataaacctaa ccttgctaaa tattgcatgt
      301 ttgctccaac ttcatctcca tacacattta caccatcata attaggatca gtaattctgt
      361 ctccattggt aatttggctg tacacattat ttctagctaa gttggcataa tctactgctt
      421 gccaatcttg ggcttgtata aattgtccag aaattttaaa tgcaaacctt tctgttactt
      481 ttttacccca acgaaaagcc caatcaaaat aaggagctgc tgtagtttgt ttgctatcca
      541 catggttaat tccttgtttt acttgaaaac ttaatccttg atatttaaaa ggatttttac
      601 tgctcattaa aatggtgcca ttcataccac cagagccata taaagcagaa gaagcaccag
      661 gtaataactc tacattatct acatctaatt ctgtcatacc aacaatatta cctactgaga
      721 aatttaaagc tggggcttga ttatccatac catcaactaa ttgattggca cgtaaattac
      781 cactaccatt aaaccctctt gtactgatag ttctaaagtt aatactagat gttgtcatat
      841 caacaccttt aaagtttaaa atagcatcgt aaaagttagg agcaactgca tttttaactg
      901 cactagcacc catacgttca atagatactg gagattctat aattttttct gatacacgag
      961 atgctgcaac aacaatctca tctcccatgg agtattttac atctaaacta acttgaacac
     1021 tttgtgaagc atctgtaatt gaaacttctt tatctgcaaa gcctacagat gaaaaaacca
     1081 aagtaaaagg aagtttttga gtagttacta atttaaattc tcctttgtca tctgtatatg
     1141 ttccagatgt ggttccttta actgtaaccg atactgccga aacagattct tttgatttgc
     1201 tgttggaaac ttttcctgaa ataatagttt gttgtgccga aactgctgct gcacaaatag
     1261 taaaaatgca cagcattagg gtaaatttga tatttctcat actacaatat tgtttaattg
     1321 tttagcaata taataacagt tagtttacta tacaaatatt tatttttgaa aattattatt
     1381 tgttttttca aattagtttt agtaacaagt ttttgtgtgt tatttttgaa tgtatgtctg
     1441 catttcaatt tccaaatcaa acaaactttt accaaggtaa agtaagagat gtttatacta
     1501 ttaataatca ttttgtagta atgattgcca gtaatcgaat ttctgctttt gatgttattt
     1561 taccaaaacc aattccattt aaaggccagg tacttaatca aattgctgct actatgttgc
     1621 aggctactaa agacatttgc cccaattggt taataaattc tcctgcccca aatgtttcta
     1681 taggaaaaaa atgtgagcct tttaaaatag aaatagttgt aagaggtaat ttaactggtc
     1741 atgcatggcg tacttataaa agtggccaaa gaattttgtg tggcattaag ttaccaaatg
     1801 gtttaagaga aaatgatttt ttcgaaaacc caattatcac cccaaccact aaagcaagtg
     1861 tagggcatga tgaagatatt agtaaagatg aaattattac gcaaaattta gcttcattaa
     1921 atgagtggga aactcttgaa aaatatgcat tacaattatt tgaaagagga aaacaaattg
     1981 ctgctaaaca aggcttaatt ttagtggata caaagtatga gtttggaaaa ataaatggag
     2041 aaattttttt aatggatgaa attcatacac ccgattcatc tagatatttt tatgctgaag
     2101 ggtttgaaga aagaattgaa aatggacaaa gccaaaagca actgagcaaa gagtttgtaa
     2161 gagaatggct tttagctaat aactttatgg gtaaagaggg acaaacagtt ccaacaatga
     2221 gtaacgagtg ggttcaaacc atttctctac gttatattga attgtacgaa aaaattttag
     2281 gagagaaatt taaacctcaa gagctatcta atgaagaaac ttatcataaa attgaacaat
     2341 ctttaaacca attaatcaat taaaaattaa ttatgaacaa acatgctttt atttttccag
     2401 gtcagggctc tcaatttaaa ggaatgggta aaaatttgta cgaaacaaat agtttggcaa
     2461 aagaaatgtt tgaacaagca aatgaaattt taaagttcag aattagtgat attatgtttg
     2521 aaggaagcga tgaagctcta aagcaaacta atgttacaca acctgcagtt ttcctacact
     2581 cagtaatagc atatagcaca ttacataatg caaagccaga tatggttgca gggcattctt
     2641 taggtgagtt ttcggccttg gtagctaata aaactttaag ttttgaagat gctctattac
     2701 tagttagtat aagggcaaat gcaatgcaaa aagcttgtga aagcaaccca agtacaatgg
     2761 ctgcagtatt agcacttgca gatgataagg tagaagaaat ttgcagttta gtacaaaagc
     2821 aatctggcga agttgtagtt gcagcaaact ataattgccc aggacagtta gtaattagtg
     2881 gaagcattaa gggtattgaa atagcttgcg aacaaatgaa agctgctggt gctaaaagag
     2941 ctttagtatt acccgttggt ggtgcatttc atagcccatt aatgtcagaa gcaaaagcag
     3001 aattagctat ggctattgaa gccaccattt ttaatacacc aatatgccca atttatcaaa
     3061 atgttgtagc aaaagggata agcgatgcta cacaaataaa acaaaactta atactacaat
     3121 taactggtgc tgttaaatgg acacaaagtg tacaagcaat ggtagcagat ggagcaactc
     3181 attttacaga agctggccct ggaaaagtat tacaaggatt ggttcaaaaa atttataaag
     3241 aggctatggt agatggcgtt agttaagtgt agtttaaaaa aatacaaagt atgttactta
     3301 agattatttt atggggtata gtaatttatt atttatacaa atttgttttt ggtgttgtga
     3361 tgcctgttag taaggctact agtgatatga agtctaaaat aaatgaaatg caaagagcac
     3421 aagaacaatt tataaagcaa cagcaacaac agcaagagca atttcaaaag caaaaagtac
     3481 atgaagaaaa tatacatcta aaaaaaggag actatataga atttgaagaa ataaaagata
     3541 aataaaagat atattatgag tattttttct aaaattattg ccggcaaaat tccatgcttt
     3601 aaaatagcag aagatgaaaa ttttttagca ttcttagata tttttcctgt gcaatatggt
     3661 catactttaa ttattccaaa aattgaaatt gataaattat ttgacctacc caatgaatat
     3721 ctttctacaa ttttagtttt tgcaaaacca gttgcacaag ctattcaaaa agcatatcct
     3781 tgtaacagag taaatattat tactgttggt ttagaagtgc cacatgcaca cattcattta
     3841 attgcaacaa atggtttaaa agatgttgat cttaacaagc caaaattaaa actaaccaat
     3901 gagcagttga aggaagttca aatgaatatt ttaaagtttt tgtagatctg ctaccatggt
     3961 gacgtacacg ttccaggcct acggagtagg aacagtaaga gaaaattacc aacttttgct
     4021 gtaaaaatga aatgtcacac gtttttatcc ctaagaagtt ggcacaatat tgccaacttt
     4081 tatttacctt ttgtaattaa taaatattat taatatggca cctcagcaaa aaagtactgg
     4141 atgatgttat aaaaacaaaa cttaaaaacg aaaaaaaact aaatgaggta acaagtattt
     4201 aaacccaatc aaaaggctta ggaaatacat taaagcagtt aaatcttaag caagtaacaa
     4261 cacagattta caggtagcaa ttttagttta aaaatttgga aagtagaatt aataaaatat
     4321 aactcagaag acaaacaaat tatttaaaca atagaaaaaa tggcaagcct taaattctgt
     4381 acatttgtta aaactctttt tatggaatac agaaaaacag caacaaaaac caaaggaaag
     4441 tctgttgcca aaaaaacatc aacaaagcaa aaaggaaatg ttgaagctgt tttaaaagct
     4501 gcaaaaacaa ataaattaga tttatcttat ttaagagaat gatagtaatt gcagatacta
     4561 cagtactttt ccatttgaat taccacaaca caaaaatgga agctattttt acttttcaat
     4621 gatgtctcca aacttctcaa ctta
//
LOCUS       DAOQPK010000177         4637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_11763_length_4637_cov_4.850502, whole genome shotgun sequence.
ACCESSION   DAOQPK010000177 DAOQPK010000000
VERSION     DAOQPK010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11763_length_4637_cov_4.850502"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2120)
                     /locus_tag="PLU36_08370"
     CDS             complement(<1..2120)
                     /locus_tag="PLU36_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46802.1"
                     /translation="MAENFMKHQLKINKTFLAIISCVYVFAQPNTDVDLYKEKPQRYK
                     ERKLGSEKTNEKKFHFLRRFTQNTYTHYNYHFNAINKLNEVVERAKMAYKDDYTHLLP
                     FYNYTIEATSAAKDDLDSVIYKCTAGILLHDLRNDWIDNLYMILGKAYLYKKDFDSAA
                     GCFQYINYTYAPKDDGYDVPLGSNASKTKGVFTISTKEKNNFWKTITSRPPSRNESFI
                     WQIRNYLEQDLLGEAAGLIGILRNDPNFPKRLKPELHEMTAYWFYKQANYDSASIHLI
                     KALSNADNKAEKARWEFLAGQLFQLANTNDSMAIVMYKKAIKHTNDPLMEVYARLNMV
                     SLASEKKQDALQENLTELLKLTKKERYADYRDIIYYAIAKIELQRNGLDAAQNYFLKS
                     IKYSIKNPQQKTLSFIALGHLNYNRKRFIESHSFYDSVKLDNAKNLDNEEKNLIAKRK
                     PALKVIAENMLAIKKEDSLQRIASMPEAERNIYVKKILKKLRKEKGLKANEEEGAYGA
                     TAVTDTKSTDMFSTDSKGDWYFVNENLKSKGFSEFKAKWGKRANIDNWRRQSSFETST
                     STKTKTRKTTKTTDEIPDTVDDIGSPRETKDDATGTEDTEGKVTEKENEVEDLSFEGL
                     MSKLPLDEERMTASHKKIQGSLFIIGVVFQDHLDEYTEAITNYQTLINKYSNFTERQK
                     TFFNLYYCYTKLGMKVQADSVLGV"
     gene            2210..2605
                     /locus_tag="PLU36_08375"
     CDS             2210..2605
                     /locus_tag="PLU36_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46803.1"
                     /translation="MENNIQQNTLKRLQNKYRLVVMNDDTYEEVITFKLSRLSVYIGF
                     STILVLMIAFTIGLIAFTPIKYYIPGYGTRQSKADLQMLKIRTDSLEKAIQYKDQYLE
                     SLKKVLTGTTPTQKDTVPLIIEKKEISTF"
     gene            complement(2663..>4637)
                     /locus_tag="PLU36_08380"
     CDS             complement(2663..>4637)
                     /locus_tag="PLU36_08380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ46804.1"
                     /translation="AGYVFTLRSTSISNTAMVTEVGAAGVIAYGTAAVFRVERYVPQT
                     GGTGVRAYRDFAPSVNTGTGTIFDNWQEGGTNGLDGGVYYGTHITGIVGSTSGYDAAT
                     GFDKTYSSLGSLSTFDVSTITGAGTWTTWSNATSRSTNQANDTISAFKGYRVLIRGNR
                     LVDLFTGPTPTTMNAPAILRSRGKLVYGDVVFNTTGVTANGATNSGIRLNSASATGYT
                     MVGNPYACPVDWELVHADAGTTNLSSTIYVFDPNLSSSGAYATYNATTHTTAPVASAA
                     NKYIQPGQAIFVKNTSVAPTLTFRETHKAPNSANLTNVFKASSDSASFSKIYINLNKQ
                     LNNLSYKIADGVAIVFDNSFNDGDGPEDASKISNNLENLSIIAKNAKQWIIEGRKQAY
                     INDTVQLRMYYGANAPVGGNYQLEINMDEFINNGLQAYLYDNYTNTLTSLGMTGISTY
                     NYNVTSTAATYNYRFSIVFKSGSVLPVSFITLNAAIQNKDVSVNWTVAESNIASYQIQ
                     RSSNAVEFTTVGTVVANGNAISSTQKYNWIDAEPLNGNNYYRIIANGKDGKTTNSSIV
                     LVRLSDNSSITVYPNPVKDRRMNLLTESLAKGDYEIQVYNLEGKNIYSSIIKHNGGTA
                     SYVINLPLAVTSGMYQMIIKGKEYTNTQNIVVE"
BASE COUNT         1450 a          791 c          664 g         1732 t
ORIGIN      
        1 aatactccta aaacagaatc tgcttgaact ttcataccta atttggtata acaatagtat
       61 aaattaaaga aggttttttg cctttctgta aagttggaat atttatttat tagtgtttga
      121 tagttggtaa tagcctctgt atactcgtct aaatgatctt gaaaaacaac accaataata
      181 aataacgaac cttgaatttt tttatgagaa gctgtcattc tttcttcatc taaaggcaac
      241 ttgctcatta atccttcaaa agataaatct tccacttcat tttctttttc tgttactttt
      301 ccttcagtgt cttctgttcc ggttgcatca tctttagttt ctcttggtga accaatatca
      361 tctacagtgt cgggaatttc atcggtggtt ttggttgttt ttcttgtttt agtttttgtt
      421 gaagtggatg tttcaaaact tgattgcctt ctccagttat ctatatttgc tcttttgccc
      481 cacttagctt taaattctga aaagcctttt gattttagat tctcattcac aaaataccaa
      541 tcgccttttg aatcagtaga aaacatgtcg gtactttttg tatctgtaac agcagttgct
      601 ccataggctc cttcctcttc atttgctttt aatccttttt cctttcttaa ttttttcaat
      661 atttttttta cataaatatt tctttctgcc tctggcatac ttgcaattct ttgtaaacta
      721 tcttcttttt ttatggcaag catattttct gcaattactt ttaatgctgg ttttctttta
      781 gcaattaaat ttttctcttc attatctaaa tttttggcat tatctaattt tacactatcg
      841 taaaatgaat gtgactctat aaaacgtttt ctattatagt ttaaatgacc taaagcaata
      901 aaactgaggg ttttttgttg tggatttttt atgctatact taatactttt aagaaaataa
      961 ttttgggcgg catctaaccc atttctttgt aattcaattt tggcaattgc ataataaata
     1021 atatctctat aatcggcata tctttctttt ttggtaagtt ttagtaactc cgttaagttt
     1081 tcctgtaatg catcttgttt tttttctgat gcaagactta ccatattaag cctggcataa
     1141 acttccatta aaggatcgtt agtatgttta atggcttttt tgtacattac aatagccatg
     1201 ctatcgttag tatttgctag ttgaaataac tgcccagcta aaaactccca tcttgctttt
     1261 tctgccttat tatctgcatt actaagggct tttattaaat gtatagatgc actatcatag
     1321 ttggcttgtt tataaaacca atatgctgtc atttcatgca attctggttt taatctttta
     1381 ggaaagttgg gatcgtttct taagatacct attaagcctg cagcttctcc taataaatct
     1441 tgttctaaat aatttcttat ttgccaaata aaactttcgt ttctgctagg tggcctagaa
     1501 gttattgttt tccagaaatt atttttctct tttgtagaaa ttgtaaaaac gccttttgtt
     1561 ttacttgcat tactacctaa aggaacatca tagccatcat ctttaggagc ataagtgtaa
     1621 ttaatatatt gaaagcaacc agcagcacta tcaaaatctt ttttgtataa atatgctttg
     1681 cctaaaatca tatacaaatt atctatccaa tcatttctta aatcatgtaa aagaatacct
     1741 gctgtgcatt tatatataac cgaatctaaa tcatcttttg ctgcagaagt tgcttctatt
     1801 gtataattat aaaatggcaa caaatgtgta taatcatctt tataagccat tttagctctt
     1861 tctactactt catttaactt attaattgca ttgaaatgat aattataatg tgtgtaagta
     1921 ttttgcgtaa atcttcttaa gaaatgaaat tttttttcgt ttgttttctc cgagcctaat
     1981 ttgcgttctt tatatcgttg cggtttttct ttgtataaat ctacatcggt atttggttgt
     2041 gcaaaaacat acacacaact tattattgct aaaaaagttt tattaatttt taattgatgc
     2101 ttcataaaat tttctgccat aaacataagc agttaaaaat acaagtattt tgcaaatata
     2161 agtttagcaa ttaagctata tcattacctt taatttcaat ttaacactca tggaaaataa
     2221 tattcagcaa aatacattaa aaagattaca aaataagtac agacttgttg taatgaatga
     2281 cgatacttac gaagaagtaa ttacttttaa actaagtaga ctaagtgttt acataggttt
     2341 tagtacaatt ttggttttaa tgatagcttt tactattgga ctaatagctt ttacaccaat
     2401 aaaatattat atacctggtt atggaacaag gcaaagtaaa gctgatttac aaatgcttaa
     2461 aataagaaca gattcattag aaaaagcaat tcaatataaa gaccaatatt tagaaagttt
     2521 aaaaaaagtt ttaacaggta ctacaccaac acaaaaagat actgtaccac tcataataga
     2581 gaaaaaagaa atttctactt tctaaaaaaa aatggctgtc tttatttaag acagccatta
     2641 ataaacagtt cagtaatagt tattactcta ctacaatatt ttgtgtgtta gtatattctt
     2701 ttcctttaat tatcatttgg tacataccag aggtaactgc taaaggcaag ttaattacat
     2761 aagatgctgt gccaccatta tgttttataa ttgaactgta aatattttta ccttctaaat
     2821 tatacacttg aatttcgtaa tctcctttag ctaagctttc tgtaagtaaa ttcattcttc
     2881 tatctttaac aggattagga tatactgtaa tagaagaatt atcggataac ctcactaaaa
     2941 caattgaact atttgttgtt tttccatctt ttccatttgc tataattcta tagtagttat
     3001 taccatttaa aggttctgca tctatccaat tatatttttg agtagaagag attgcatttc
     3061 catttgctac aacagtacct actgtagtaa actctactgc attactactt ctttgaattt
     3121 gataagatgc tatattagat tctgcaacag tccaattaac agaaacatct ttattttgaa
     3181 tggctgcatt aagtgtaata aacgacaccg gtaaaacact gccagattta aacacaattg
     3241 aaaagcggta attataagtt gctgctgtag atgttacatt gtagttatag gtagaaattc
     3301 ctgtcatacc taaactagtt aaagtgtttg tataattatc gtataagtat gcttgaagcc
     3361 cattgttaat gaactcatcc atatttattt ctaattggta attaccacca acaggtgcat
     3421 ttgcaccata atacatacgt aactgaacag tatcattaat gtatgcttgt tttcttcctt
     3481 cgattatcca ctgtttagca ttttttgcta taatacttaa attttcaagg ttgttactta
     3541 ttttgcttgc atcttcagga ccatctccat cattaaagct attatcaaaa acaatagcaa
     3601 ccccatcggc tattttatag cttaaattat ttaattgctt attcaaatta atataaattt
     3661 tagagaaaga tgcactatca ctacttgctt taaacacatt agtaaggttt gcagaattgg
     3721 gtgctttatg tgtttctcta aaagttaaag ttggggctac ggaagtgttc ttcacaaaaa
     3781 ttgcctgacc tggttgaata tatttattag ctgcagaagc aactggtgct gtggtatgtg
     3841 ttgttgcgtt gtaggtagca taggcaccac ttgaagacaa gtttggatcg aatacataga
     3901 tggttgaaga aaggtttgta gtacctgcat ctgcatgtac taattcccaa tcaactgggc
     3961 aagcataagg gttaccaacc attgtataac ctgttgcaga ggcagaattt aaccttattc
     4021 ctgaattagt agcaccatta gctgtaacac cagttgtatt aaacactaca tctccataca
     4081 ctagcttacc tcttgaacgt aaaatagctg gagcattcat tgtagtaggt gttggtccgg
     4141 taaataaatc aacaagccta ttaccacgta ttaaaactct atagccttta aatgcagaaa
     4201 ttgtatcgtt tgcctggtta gtagaacggc tagtagcatt gctccaagtt gtccaggttc
     4261 cagcaccagt aattgtactc acatcaaaag tacttaatga acctaaagaa gagtatgttt
     4321 tatcaaagcc agttgcagca tcgtacccac tagtgctgcc tacaatacca gtaatatgag
     4381 taccataata aactcctcca tctaatccat tggtgcctcc ctcttgccaa ttgtcaaaaa
     4441 tagttccagt accagtgttt acactaggtg caaaatctcg gtatgctctt acaccagtac
     4501 cacctgtttg aggtacatac ctttctactc taaacacagc tgcagttcca taagcaatta
     4561 cacctgcagc accaacttct gttaccattg cagtatttga aatggaagtt gaacgtaaag
     4621 taaatacata gcctgct
//
LOCUS       DAOQPK010000178         4620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_11836_length_4620_cov_4.381599, whole genome shotgun sequence.
ACCESSION   DAOQPK010000178 DAOQPK010000000
VERSION     DAOQPK010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11836_length_4620_cov_4.381599"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3884)
                     /locus_tag="PLU36_08385"
     CDS             complement(<1..3884)
                     /locus_tag="PLU36_08385"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HMZ46805.1"
                     /translation="MKYIKFIFLLAYCISSSFVANAQTFNNEWIDFSKTYYKFKVASS
                     GLYRINKTDLPTNLQNTDAAFFQLWRNGQQVTLYTSVANGTLPNNGFIEFLGIKNDGI
                     PDKPLYREASFQLSDKISLQTDTACYFLTVNTVASQNYRYSNGINNVAGNSLAPEPFF
                     IYTKQFDFKESIHRGHGENAGERVTSSSYDIGEGWGSNEIYASSPYTFSTSNLYPYST
                     GPNASIKMAVNGVSYLNRNVTLKLNNTAYINNQLLTAQQSAVFSSSVPMTAINNSTND
                     FSISINPVNIADNVNRIYSAYVSLTYSRLFNFGNQTNFDFTIAASANPAGTYIEINNF
                     NSAAIPVLYDLVNNIKYTAVVENGIIKFALPFSSTSRNFVLVSQDISAIQTINNFQEK
                     TFINYSLTSNQGNYLIVSNKIAGINAGEPVDQYRQYRTSLAGGGFNAKIYDIDDLVDQ
                     FAFGIKKHPLSVKNFVRFAKQNFTTNPKQIFIIGKAVAYDEYAVNQSNLNADKLNLVP
                     TFGWPASDALLVSDGVNPQPLLLVGRLSVVNPNEVSIYLQKVQQYEQEAANNVQTVAN
                     KLWQKQIVHVAGGNDPNIEPLLIAYLNGYKAIIKDTLFGGSVADFNTVATGGGATPAV
                     VDLLKNYFKNGIALLTYFGHSAATQLDYNLNNPLDYNNPGKYPMFLLNGCNAGNFFDY
                     DESRLNNITSFSEKFVFANQHGAIGVIASSHFGLTGYLNSYSTGFYNSIRTSTGYNNY
                     VGKNMMDAMAPFSFNDFFSRIHAEQFILHGDPAIKVYAKDKPDFAVEENSVTINPTVL
                     SVADNNFTLKAKLFNIGKSQAAKTVNAGDSVHVIVKWQHGNGSVDYLFNGFIKPSIRN
                     TDSLILNASISPLRDKGNNCITIVIDSLNQYNELSKLNNTVSKCFFIFDDDIKPVYPY
                     NFSIVNTNTIKFFATTANPIAAAKDYVMEIDTTELFNSALKITHFKNSVGGAIEFNPS
                     IALTDSTVYYWRVAAVPNSGETRWNNASFVYLNGTETGYNQSHLYQHFKSSFNRIYID
                     SFSRNWNYGLAATNLNITQGVFPYSPYDADYSVSKNGKYVVSSGCLGYAVRFTLFDAT
                     TMKPYYNQALPALDSVGASGGFMGSSAVNCAKPGRQYNFEFSYNSLLERNKMRDFINW
                     IPNNVVAVARVNLDLPFDANPVAVWMNDSIANGSIQNTLYGKLKENGFLNIDSFNFPR
                     SWIFAFQKGNAAFTPKSAFGFDINDIAHLDVTVNSTDSLGYVTSPILGPAKQWKTLKW
                     RGKTSDLSAGDIPLMDIVGINNSGVETVLMTN"
     gene            4297..>4620
                     /locus_tag="PLU36_08390"
     CDS             4297..>4620
                     /locus_tag="PLU36_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ46806.1"
                     /translation="MRQLKIATQITNRDSQAVEKYLQEISKIPMITPEEETTLAQKIK
                     MGNQRALDKLVQANLRFVVSVAKQYQHQGLSLSDLINEGNLGLIKAAQRFDETKGFKF
                     ISYAVW"
BASE COUNT         1657 a          763 c          683 g         1517 t
ORIGIN      
        1 atatttgtca ttaatacagt ttcaacacca gaattattta taccaacaat atccattaaa
       61 ggaatatctc ctgcagataa atcacttgtt ttacctcgcc attttaatgt tttccattgt
      121 tttgctggtc ctaaaattgg agaagtaaca tagcctaaag aatcggtgga attaactgtt
      181 acatctaaat gagcaatatc gttaatatca aaaccaaaag cagattttgg agtgaaagca
      241 gcattgcctt tttgaaaagc aaaaatccat gaacgaggaa aattaaatga atcaatattt
      301 aaaaagccat tctcttttaa cttgccataa agagtgtttt gaatagagcc attagctata
      361 gaatcattca tccaaactgc aacaggatta gcatcaaaag gtaaatctaa gttcactctt
      421 gcaacagcca ctacattatt tggaatccag ttgataaaat ctctcatctt atttctttcc
      481 agtaaagagt tataactaaa ttcaaaatta tactgcctac ctggctttgc acagtttaca
      541 gcactgctac ccataaaacc tccagaagca ccaacagaat ctaatgctgg taatgcttgg
      601 ttataatatg gtttcatggt agttgcatca aacaaagtaa aacgaacagc ataacctaag
      661 cacccacttg atacaacata ttttccattt tttgaaacag aataatccgc atcgtatgga
      721 gagtaaggaa atactccttg tgtaatattc aagttagttg ctgccaatcc ataattccaa
      781 tttcttgaga aactatctat atatattcta ttaaaactgc ttttaaaatg ttggtataaa
      841 tgcgattgat tatagccagt ttctgttcca tttaaataca caaaacttgc attattccat
      901 cttgtttccc cactattagg tactgcagca acacgccaat agtaaactgt tgaatcagtt
      961 aaggctatag atggattaaa ttcaatagcc ccaccaaccg aatttttaaa atgggtaatt
     1021 tttaaagcag aattaaacaa ctccgtagta tctatttcca tcacataatc ttttgcagca
     1081 gcaattgggt ttgcagtagt ggcaaaaaat tttattgtat tggtatttac tatagaaaaa
     1141 ttataaggat aaacaggttt aatatcatca tcaaaaataa aaaagcattt gcttacagta
     1201 ttatttagtt tactcaattc attgtactgg tttaaactat ctataactat ggtaatacaa
     1261 ttatttcctt tatctcttaa aggagaaata cttgcattta atattaaaga atcggtatta
     1321 cgaatagagg gtttaataaa accattaaat aaataatcta cacttccatt accatgttgc
     1381 cattttacaa taacatgaac agagtcgccg gcatttacag ttttagcagc ctgagatttt
     1441 ccaatattaa aaagtttggc tttaagtgtg aaattgttat cagcaaccga aagaactgtt
     1501 gggtttatag ttacactatt ttcttctaca gcaaaatctg gtttatcttt tgcataaact
     1561 tttatggctg gatctccatg taaaataaat tgctcagcgt gaatacgaga aaaaaaatcg
     1621 ttgaaagaaa aaggagccat ggcatccatc atattttttc caacataatt attgtaccct
     1681 gtagaagttc ttatggaatt ataaaaacca gtagagtaag agtttaaata acctgttagc
     1741 ccaaagtgag agctggcaat tactccaatt gcaccatgct ggtttgcaaa tacaaatttt
     1801 tctgagaatg aagtaatatt atttaatcta gattcatcat aatcaaaaaa gttacctgca
     1861 ttgcacccat ttaataaaaa cattggatat tttcctgggt tgttataatc taatggatta
     1921 ttcaaattat aatctagttg tgtagcagca ctatgcccaa aataagttag taaggctata
     1981 ccatttttga agtaattttt taataaatca actactgctg gagttgcacc accaccggtt
     2041 gcaacagtat taaaatctgc tacactacca ccaaacaaag tatctttaat aatagcttta
     2101 taaccattta aataagcaat taataaaggt tcaatattag gatcgttgcc acctgcaaca
     2161 tgtactattt gtttttgcca aagcttatta gctacagttt gtacattgtt ggcagcttct
     2221 tgttcgtatt gttgtacctt ttgtaaataa atgcttactt cattagggtt tactacagat
     2281 aacctaccta ccaaaagtaa aggttgcgga tttacaccat ccgaaaccaa taatgcatct
     2341 gaagcaggcc agccaaacgt tggaactaaa tttagtttat ctgcattaag gttactttgg
     2401 ttaactgcat attcatcata cgcaactgct ttaccaataa taaatatttg tttagggtta
     2461 gtggtaaaat tttgcttggc aaaacgtaca aaatttttta cacttaaagg gtgtttttta
     2521 attccgaaag caaactgatc tactaggtca tctatatcat atattttagc attaaaacca
     2581 ccgccagcaa ggcttgtacg atactgacga tattgatcta caggctctcc agcattaatg
     2641 cctgcaattt tattagatac aattaaataa tttccttgat tactagttaa gctataattg
     2701 ataaatgttt tctcttgaaa attatttatt gtttgtatag ctgaaatatc ctgacttact
     2761 aaaacaaaat ttcttgatgt agaagagaaa ggcaatgcaa attttataat accgttttct
     2821 acaacagcag tatatttaat attatttact aaatcgtaca aaacaggaat agctgcacta
     2881 ttaaaattat ttatctctat ataagtacca gcagggttgg cacttgcagc aatggtaaaa
     2941 tcaaaattag tttgatttcc aaagttaaat agccttgaat aggttaaact tacataagcc
     3001 gaataaattc tgttaacatt atctgctata ttaactgggt taatagaaat gctaaaatcg
     3061 tttgtagaat tatttattgc agtcatagga acactactac taaaaacagc agattgttgt
     3121 gccgttagca actgattatt tatgtaagca gtattattga gttttaatgt aacatttctg
     3181 tttaaataag aaacgccatt aactgccatt tttatacttg cattaggacc tgttgaataa
     3241 ggatataaat tggaagtaga aaaagtatag ggcgaacttg catatatttc atttgaaccc
     3301 catccttcac caatatcata agaagaagag gttactcttt ctcctgcatt ttctccatga
     3361 cctctatgaa tactctcttt aaaatcaaat tgttttgtat aaataaaaaa aggctcaggg
     3421 gctagtgaat ttccagctac attattaatg ccattggagt aacgataatt ttgagaagca
     3481 acggtattta ctgttaaaaa ataacaagca gtatctgttt gtaaactaat tttatcggag
     3541 agttgaaaac tagcctccct atacaaaggt ttatcaggaa ttccatcgtt ttttatgccc
     3601 aaaaactcaa taaaaccatt attaggcaaa gttccattag caacacttgt atataaagta
     3661 acttgttgcc catttctcca taactgaaaa aaagcagcat ctgtattttg taaattagtt
     3721 ggtaaatcgg ttttattaat tctatacaaa cccgaagaag caactttaaa tttataatag
     3781 gttttactaa aatctatcca ctcgttatta aatgtttggg cattagcaac aaaagaacta
     3841 cttatacaat aagccaataa aaaaataaat tttatatact tcattatacc aattaattgt
     3901 tttcttaacg tacaacaaaa aaataatttt cttttgtacg aataggctac gaatataaat
     3961 atttgtgcat tacaagaata aaaaatagaa aagctaacaa tgtaaagaga acaataatct
     4021 tcaaactgct tctttcaata aaaaagatta tttgagatta aaatgggtat tttgaatata
     4081 agccatactt cacaaaaggc ttaaaatgag ctaattaata aatattatcg ttaaataata
     4141 ggtttatttt caaaaaatat aaataattaa ggattaaaat gtaatttcgc tgccatttta
     4201 ttttattcaa tagttatttt tttaaatcta ttgcaacgaa aataatggaa aattagtcta
     4261 atagctagca agtaaaagta acaagaaaaa tacactatga ggcagctcaa aattgcaacc
     4321 cagatcacca atcgagattc acaagccgta gaaaagtatc tgcaggaaat ttctaaaatt
     4381 cctatgatca ctcccgaaga ggaaacaaca ctggcacaga aaattaagat gggtaatcaa
     4441 cgtgcacttg acaagttggt gcaagcaaac ttacgttttg tggtatctgt tgccaagcaa
     4501 taccagcacc aaggtctttc tttaagcgat cttatcaacg agggaaatct cggccttata
     4561 aaggcggctc aacgctttga tgaaacaaaa ggctttaagt ttatttctta tgccgtttgg
//
LOCUS       DAOQPK010000179         4619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_11838_length_4619_cov_5.300833, whole genome shotgun sequence.
ACCESSION   DAOQPK010000179 DAOQPK010000000
VERSION     DAOQPK010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11838_length_4619_cov_5.300833"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4087)
                     /locus_tag="PLU36_08395"
     CDS             complement(<1..4087)
                     /locus_tag="PLU36_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HMZ46807.1"
                     /translation="MKKLCSLMLTNGKILLLFFVILFSSKNSFSQVYYLQDWDVGDLN
                     SWTTTNSGFARTTITPCVVASVRANINSSIQKMLISPLLGTSNGDQVTLNFKYKIINV
                     SGGGATANNFGNLKMEYASNIAGPWTTIQTISNDHIPSVNCISKSVQFTPPSGNLYIR
                     FNPTWAVGNYYMYFDDVSVLQGNCFPPTNLTVLTTTNNAAILNWTAPIPAPSGGYNWE
                     VRTSGAPGSGPTGLVSSGNEPSTTSTVTGLTGQTTYTYYLRSYCGGANTSGWVSTPFT
                     TLCDTISTYPFTEGFNTPGNLSSCWSVYEGNINATQHWEPVANDVTHGAASPAEGAGF
                     MRMNYFNAFAFYNPYYLQSNSFNLGSQKKVAKFALWMGANSGLNNLRFEISNDGGGTW
                     VTLAGYTADPSNNNASAPWDNKVISLASYVNQTVMFRLRASSGNGLNGYCNIGFDNFV
                     IENAPPCAEPTSLSANTITTSSAKINFSATPGSFILEYGLSGFSPGSDNTAGVGGSII
                     LGSSSPITITGLTSNTLYDVYIRRDCGSGIYSINSIKTSFTTLCTVVSTYPFYEGFNS
                     ATLPTCWQASEGNAGANQHWQPVTTDVTHGAASSSEGSHFLRMNYYLAQPFYNPYYLK
                     SKSFNLGSVPRRAKFALWMGSSSGNNNLIFEISNNGGVTWTTLATYSANPANNGPNTP
                     WENKSVNLSSYLNQTVIFRLNATSNYGENYCNIGFDNFIIEDLPSCVEPTSLSISSIT
                     TTSASVAFASSGSSFIVEYGVAGFTPGSNNNPGVGGTIVTGTSSPIAISSLTPSITYD
                     VYVRQDCGGVFSGNSIKASFTTLCNPVTTFPYNEGFESGSMPVCWSKQYVQNTVDWTF
                     VATNNNNSITPRTGTKMANFFSNSKNMVTKLITPKLNLTSLTMPTLRFFYANVAWAGD
                     VDQLRIYYKTSDVGSWVLIPGAVYTTQHTSWTEVKLVLPAPSANYYIAFEGNAQWGRG
                     VEVDDVSIGEYASSNTDYFRTKSSGNWNEANIWQTSVDNVEWFDATLAPDYYASGVYI
                     TNNKTVTITAPVIIDKTFVAANGKLIVNNALSIINNGLTLQSSAEGTASIGESSGVIT
                     GNLVVERFIPAKATRKWSFLSSPVTQSLSNSWQQQIHITGAGTGGTICPSLTQHSNGY
                     DATLTNSPSIYTYNAAAAPGARWTTLNGTTSNSLGAGKGFRVNVRGPRSIGCNLLNGV
                     INSTSAVTLRSIGSVNNASKNSGGFNLIYPNNGVNNYFFFGNPYPSAISFSALQALNA
                     FSISSSYAVYVPSNASGVYSYWSDDDGFFTGGSGYDDNKGNIIASGQAVFLQSTVPGS
                     VTLEIDETIKDSESTAGYFKANNTKDRVRISLNKDSQIDEVV"
BASE COUNT         1514 a          834 c          733 g         1538 t
ORIGIN      
        1 ttaccacttc atctatctgg ctgtctttgt ttaagcttat tcttactctg tcttttgtgt
       61 tattagcttt aaaatatcct gctgtagatt ctgaatcttt aatggtttca tcaatttcta
      121 aagtaacact acctggtaca gttgattgta gaaaaacagc ctgaccgctg gcaataatat
      181 ttcctttatt atcgtcatag ccagagcctc ctgtaaaaaa tccatcatca tcgctccagt
      241 aagagtaaac acctgatgca ttggatggaa catagactgc atagctagaa gatattgaaa
      301 atgcatttaa agcttgtaaa gcactaaatg aaatagcact tgggtaaggg tttccaaaga
      361 agaaataatt atttacccca ttatttggat atattaaatt aaagcctcca ctgtttttag
      421 atgcattatt aaccgaccct atagatctta atgtaacagc acttgttgaa ttaattactc
      481 cattcaataa attacaacca atgcttctag ggccacgaac atttactcta aaacctttac
      541 ctgcacctaa actattggag gttgtgccat ttaaagtagt ccaccttgca ccaggtgctg
      601 ctgcagcatt atatgtataa atacttggag agttggtgag tgtagcatca tacccattac
      661 tgtgttgagt aagtgaagga caaatggttc caccagtacc tgctcctgtt atatgtattt
      721 gctgttgcca actgttagat agagactgag ttacagggct tgataaaaaa ctccattttc
      781 ttgtggcttt tgcaggtata aatctttcaa ctaccaaatt gcctgtaatt acacccgatg
      841 attctccaat gcttgcagtt ccctcggcac ttgactgtaa ggttaaccca ttattaataa
      901 tggatagggc attatttacg attagttttc cattggctgc tacaaatgtt ttatctataa
      961 ttactggtgc tgtaatagtt actgttttat tgtttgtaat atatacacca gaagcataat
     1021 aatctggagc taatgttgca tcaaaccatt ctacattatc aacagaagtt tgccaaatat
     1081 ttgcttcatt ccaattgcca gaagatttag ttctaaaata atcggtattt gaactagcat
     1141 attctcctat tgaaacatca tctacctcaa caccacgccc ccactgtgca tttccttcaa
     1201 atgcaatgta ataattagca gaaggtgctg gtagcacaag ttttacttct gtccaactgg
     1261 tatgttgtgt agtataaaca gcaccaggta ttaaaaccca acttccaaca tcgctggttt
     1321 tataataaat tcttaactgg tcaacatcac cagcccatgc tacattagca taaaagaacc
     1381 ttaatgttgg catcgttaaa gaagttaagt ttaattttgg agtaattagc ttagtaacca
     1441 tgttcttact attagagaaa aaatttgcca ttttagttcc tgttcttggt gtaatactgt
     1501 tattattatt tgtagctaca aatgtccagt caacagtatt ttgaacatat tgcttactcc
     1561 aacaaaccgg catactacca ctctcaaaac cttcattata tggaaaagta gttacaggat
     1621 tacataatgt tgtaaagctt gccttgatac tattgccact aaaaacgcct ccacaatctt
     1681 gccttacata aacatcgtaa gtaatacttg gtgttaatga agatattgct attggagaag
     1741 atgtaccagt aacaattgtt ccacctactc caggattatt atttgaacca ggtgtaaagc
     1801 ctgcaactcc atattccact ataaaagaac taccactact agcaaaagca acggatgcag
     1861 atgttgtagt aatggaacta atagataacg atgtaggttc tacacaggat ggaaggtctt
     1921 caataataaa attatcgaaa ccaatattac aataattttc cccatagtta gaagttgcat
     1981 ttagcctaaa tattactgtt tggttaagat aggaagacaa gtttacagat ttattttccc
     2041 atggtgtatt agggccatta ttagcaggat ttgcagaata ggtagctaat gttgtccatg
     2101 ttacaccacc attattgctt atttcaaaaa ttaaattatt gttaccactg ctagaaccca
     2161 tccaaagtgc aaattttgct ctacgaggta cagaacctaa attaaatgat tttgatttta
     2221 aataataagg attataaaaa ggctgagcca aataataatt catacgcagg aaatgggatc
     2281 cttccgaaga actagctgca ccatgtgtaa catctgtagt tacaggttgc cagtgttgat
     2341 ttgctcctgc atttccctca gaggcttgcc aacaagttgg tagtgttgca gaattaaaac
     2401 cttcgtaaaa aggataggta ctaacaactg tacacagtgt tgtaaaacta gttttaatgc
     2461 tgtttatact atatatgcca ctaccacaat ctcttcttat ataaacatca tacaatgtat
     2521 tgcttgttaa acctgttatc gtaattggtg aagaggaacc taggataatt gaacctccta
     2581 cccctgcagt attatcacta cctggcgaaa aaccagaaag tccatattct aaaataaatg
     2641 aaccaggcgt tgcactaaaa ttaattttag cagacgaagt tgttatagta tttgcagata
     2701 aagaagtagg ctctgcacat ggtggtgcat tttcaattac aaaattatca aaaccaatat
     2761 tacaatatcc atttaaacca ttgcccgatg aggctctcaa cctaaacatt actgtttgat
     2821 taacataaga ggctaaactg ataactttat tatcccaagg tgcagatgca ttgttgttgg
     2881 aagggtcggc agtatatcca gccaatgtta cccatgttcc accaccatca ttgcttatct
     2941 caaatcttaa attatttaat ccactgttgg ctcccatcca taatgcaaac tttgcaacct
     3001 ttttttgaga acctaaatta aatgaattag attgtaaata atatggatta taaaatgcaa
     3061 aggcattaaa gtaattcata cgcataaaac cagccccttc tgcagggctt gcagctccat
     3121 gagttacatc atttgctaca ggctcccaat gttgtgtggc atttatgttt ccttcgtaaa
     3181 ccgaccaaca agaagataaa ttacctggag tattaaatcc ctcagtaaaa ggatatgttg
     3241 aaatagtatc acataaagta gtaaatggag tggaaaccca gccgctcgta tttgctcctc
     3301 cacaataaga acgcagataa taggtataag ttgtttgacc tgttaaacct gtaactgttg
     3361 atgtagtgct tggttcattt ccactactta ctagtccagt tgggccacta cctggtgcac
     3421 cacttgttct aacttcccaa ttataccctc cacttggtgc aggtattgga gctgtccagt
     3481 ttaatatagc tgcattattg gttgtagtaa gtacagttaa attagttggg ggaaagcaat
     3541 tgccttgtaa tacacttaca tcatcaaaat acatataata gttacctact gcccacgttg
     3601 ggttaaatct aatatacaag ttaccagatg gtggtgtaaa ttgtactgat tttgaaatac
     3661 agtttaccga tggtatatga tcattgctta tagtttgaat ggttgtccac ggacctgcaa
     3721 tattgcttgc atattccatt tttaaattac caaaattatt ggctgtagca ccgccgcctg
     3781 aaacatttat tattttatat ttaaaattca aggttacctg atctccatta ctagtaccta
     3841 ataaaggaga gataagcatt ttttgaatac tcgaatttat gtttgccctt acagatgcaa
     3901 ccacacaagg ggtaatggtt gttcttgcaa atccggaatt agtagttgtc catgaattca
     3961 aatcacctac atcccaatcc tgtaaataat aaacctgtga aaatgaattt ttagaagaaa
     4021 aaagaataac aaaaaatagt aatagaattt ttccattagt gagcattaaa ctgcaaagct
     4081 ttttcatata aaaagttttg gtaaactaaa attttaatta atcagaaaat ttagttttta
     4141 taattgaggt tatgcaattt aagcaatttg agtattatac aaaaaaaata gtacttaaac
     4201 tgcttttact caattttcga taattataaa accaatctgt caacttcaat taataaaaac
     4261 taaaaaataa attttacccc tgataatttt ttaaacaact atgacctata aaaactccca
     4321 tagtttaaac atcccttcaa taaaaaataa ttattggcca aacttatata ttaaataagt
     4381 tcttcatttc tatattaaaa tctttcatta taaataattg aagtataaag ttaatttatt
     4441 tggttttgag tttgtaacaa taaaaatccc ttgcatttat gcaagggatt tttattgttt
     4501 ttgttattat ttaatgaggt tatattgtaa ttaattaaaa ttacagttat tgtagtagtt
     4561 gttaaacagg ctgcttagtt tttattctgt tgcttctttc tgttatctct taacttctt
//
LOCUS       DAOQPK010000180         4548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_12151_length_4548_cov_4.239706, whole genome shotgun sequence.
ACCESSION   DAOQPK010000180 DAOQPK010000000
VERSION     DAOQPK010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12151_length_4548_cov_4.239706"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PLU36_08400"
     CDS             <1..526
                     /locus_tag="PLU36_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HMZ46808.1"
                     /translation="RYTQGFIKQEELRWKRIWLTFLEYKIANEPLSKQIATDFLQILP
                     TKKILFPYTIEILQYLTKKNYLLHLITNGFEAVQNNKLKNSNIHSFFKEVITSQGSNS
                     LKPNKEIFEYALNKSKASVHESLMIGDNLEADIVGGINIGMDTVYVNYLNIKASNIQP
                     TYTITHLQQLEEIL"
     gene            653..1252
                     /locus_tag="PLU36_08405"
     CDS             653..1252
                     /locus_tag="PLU36_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HMZ46809.1"
                     /translation="MIKTGNPIISIYTEMTPNPETMKFVANKLLYPGKSIDFQDESSA
                     SPSPLAKELFSFPFIKSVFIASNFITLTKTSETEDWDDIIPTIKQFLKDYLENGGVVV
                     NEEEAAKVKQESSNTVSADDNDIVKRVKELLENYVKPAVEMDGGAIQFKSYNDGVVNL
                     MMQGSCSGCPSSMITLKAGIEGMMKRMIPEVKEVVAEAE"
     regulatory      1415..1528
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1710..2876
                     /locus_tag="PLU36_08410"
     CDS             1710..2876
                     /locus_tag="PLU36_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HMZ46810.1"
                     /translation="MKETTKILHGIPVDELTGAIATPIYQTSTYVQIAPGVHKGYDYA
                     RSNNPTRKVLEDLIASLEEGKNGFAFASGLAAIDAVVKLLQFGDEIIAVEDIYGGAFR
                     LFTHIYQKFGIHVNYVDSTNALNIENAITPKTKLIWIETPTNPTLKISDIQAIAKIAK
                     ANKVLLCVDNTFASPAVQKPIAFGADIVIHSATKYLAGHSDLIAGLVVTNSEELGEKI
                     KFIQNATGAILAPFDSWLVIRGIETLPLRVKQHSETALKIATFLQQQDIVNNVYYPGL
                     QEHKNYDIAKKQQKYFGGIISFDLKIEDKILATQLVSNTTYFKLAESLGGVKSLICLP
                     CEMTHKSIPLEKRHQSGVTDSLIRLSVGLEDAEDLINDLKTVFQTIAKKISIKA"
     gene            2886..3995
                     /locus_tag="PLU36_08415"
     CDS             2886..3995
                     /locus_tag="PLU36_08415"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HMZ46811.1"
                     /translation="MNRKQLTIGLFGFGCVGKGLYEVLQKTPALKASIKSICVKTKNK
                     QRSLSNQYFTFEKNDILQDDTINTIVELIDDADAAFEIVKTALQKGKAVVTANKKMIA
                     EHFEELLHLQKQYNVPLLYEAACCASIPIIRNLEEYYDNDLLESIEGIVNGSTNYILT
                     KTVEKNISFSLALKEAQQKGFAETNPLLDVGGFDAKYKLQILLAHAFGVITQPQNIFN
                     LGINNLYDFELNYAKEKGYKIKLVAYASKNEEGKIISFVLPKFVTSKDKLFSVDDVFN
                     GVKIQSYFCDNQFFVGKGAGAYPTSSAVLSDISALTYDYRYEYKKLNTQHSLANEEDI
                     FLKIFFCVFPYQLILSLNHFLKILKNTISMKTMAL"
     gene            4125..4529
                     /locus_tag="PLU36_08420"
     CDS             4125..4529
                     /locus_tag="PLU36_08420"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HMZ46812.1"
                     /translation="MNLVTKKIVMAKDIGIHGNLFGGILMAWIDEAAAAYATEYCHTP
                     NMVTVHVDAFTFKKPLKAGQHIRIYACVKELGRTSITLKLEAYKYNLYSREETLVCNT
                     NATFVRIDDDGTPTAIGETIRRNHYEKLPITT"
BASE COUNT         1764 a          616 c          733 g         1435 t
ORIGIN      
        1 tagatataca cagggtttta ttaaacaaga agagttgaga tggaaaagaa tttggctaac
       61 ttttttagaa tataaaattg ctaacgaacc cttatcgaaa caaattgcta ccgatttttt
      121 acaaatttta cctactaaaa aaattctatt tccttatacc atagaaatac tgcaatatct
      181 aactaaaaaa aattacctac tacatttaat aaccaatggt tttgaagcag ttcaaaacaa
      241 taaactaaaa aatagtaata ttcattcatt ttttaaagag gtaattacat cacaaggtag
      301 taacagctta aaacctaata aagaaatttt tgagtatgct ttaaataaat ctaaagcaag
      361 tgtacatgaa agtttaatga ttggagacaa tttagaagca gatatagtag gaggtataaa
      421 tattggaatg gatacagtgt atgtaaatta tttaaacatt aaggcaagta atatacaacc
      481 aacttatacc attacccatt tacaacaact agaagaaatt ttatagaaac taattgtata
      541 aattgactgt taaaaaaact attacgataa ccgaaaaaag caaaatgctt acaaaacaat
      601 gattattgct ataaattaat gctgtaactt tgtagcgttt aaattttttg ttatgataaa
      661 aacaggaaac ccaattatta gtatttatac agaaatgaca cccaacccgg aaacaatgaa
      721 atttgttgcc aacaagttat tgtaccctgg taagagtata gactttcaag atgaaagtag
      781 tgcaagccca tctcctttag ctaaagaatt gtttagtttt ccgtttatta aaagcgtgtt
      841 tattgcaagt aattttatta ctctaacaaa aacatcagaa acagaagatt gggatgatat
      901 tattccaaca attaaacaat ttttaaaaga ttatttagag aatggtggtg ttgttgttaa
      961 tgaagaagaa gctgcaaaag taaaacaaga gtcaagcaat actgtaagtg ccgatgataa
     1021 tgatatagta aaaagagtaa aagagttgct agaaaattat gtaaaacctg cagttgaaat
     1081 ggatggtggt gcaattcagt ttaaaagcta taacgatggt gtagtaaact taatgatgca
     1141 aggaagctgt agtggttgcc ctagcagtat gattacttta aaagcaggta ttgaaggaat
     1201 gatgaaacgc atgattcctg aagtaaaaga agtagttgca gaagcagaat aatacttttt
     1261 taacacctat caattttcat agagcatcca tttaattggt tttttaaacc aattaaatgg
     1321 atgctagttt tttaaagagg tacatttctt ttttaaaaaa aaatttggaa gtttaatttt
     1381 tatactttct tttgcactta gttctttaaa aatattctta tccagaaaga cagagggaat
     1441 tgacccgatg acgtcttgac aacctctttg cctcaagcaa agaaaggtgc caaattcaac
     1501 tcagcagtaa tgccgagaaa gataagttag aaaatatagc aattaataac cgttaaaaaa
     1561 cggcaaataa tattaaaggc tcttctaact tgttggaaga gctttttaat tataaagatc
     1621 ttccacaagt tgtattctat ctgtgttttg gattagattt tttttgatga acagtaataa
     1681 aatgtttaac aaattaattc aaaacaaaaa tgaaagaaac aacaaaaatt ttgcacggca
     1741 tacctgttga tgaattaaca ggtgcaattg ctaccccaat ttatcaaaca tctacctatg
     1801 tgcaaatagc accaggtgta cacaaagggt acgactatgc cagaagtaat aaccctacaa
     1861 gaaaagtttt agaagattta attgcaagtt tagaagaagg aaaaaatggt tttgcttttg
     1921 ctagtggctt ggctgctata gatgcagtag taaaactatt acaatttggc gatgaaataa
     1981 ttgctgttga ggatatttac ggaggagcct ttcgtttatt tacacatatt tatcaaaaat
     2041 ttggtattca tgtaaattat gtagattcaa caaatgcatt aaatatagaa aatgccatta
     2101 ctcctaaaac taaattaatt tggattgaaa ctcctactaa tccaactctt aaaatttcag
     2161 atatacaagc tattgcaaaa attgctaaag ccaataaagt attgctttgt gttgataata
     2221 catttgcttc tcctgcagta caaaaaccaa ttgcatttgg agcagatatt gtaattcatt
     2281 ctgcaacaaa atacttagca gggcatagtg atttaattgc aggattggtt gtaaccaata
     2341 gcgaagaatt gggagaaaaa attaaattca ttcaaaatgc taccggtgct attcttgctc
     2401 cttttgatag ttggcttgtt attcgtggta ttgaaacttt acctcttaga gttaagcaac
     2461 attctgaaac agccttaaaa attgctacat ttttacaaca acaagatatt gtaaataatg
     2521 tttattaccc agggttacaa gaacataaaa attatgacat tgctaaaaag caacagaaat
     2581 attttggcgg tataataagt tttgatttga aaatagagga taaaatttta gcaacacaat
     2641 tagttagcaa tacaacatat tttaaactag cagaaagttt aggtggtgta aaaagtttaa
     2701 tatgcttgcc ttgcgaaatg acacacaaat ctattccact agaaaaaaga caccagtctg
     2761 gtgtaacaga tagcttaatt cgtttgtctg taggattaga agatgcagaa gatttaatta
     2821 atgatttgaa aacagtattc caaacaatag caaaaaaaat ttccatcaaa gcttaattta
     2881 aaaaaatgaa tagaaaacaa ttaaccatag ggctttttgg gtttgggtgt gttggtaaag
     2941 gtttgtatga agtattacaa aaaacacctg ccttaaaagc aagcattaaa agcatttgtg
     3001 taaaaactaa aaataaacaa aggtctttat ctaaccaata tttcacgttt gaaaaaaacg
     3061 atattttaca ggatgataca attaatacaa tagttgaatt aattgatgat gcagatgctg
     3121 catttgaaat tgtaaaaaca gctttgcaaa aaggaaaggc tgttgtaact gcaaataaga
     3181 aaatgatagc tgagcatttt gaagaattac ttcatcttca aaagcagtat aatgtaccat
     3241 tgttgtatga ggctgcatgc tgtgctagca ttcctattat tagaaattta gaagaatatt
     3301 acgataacga tttactggaa tctatagaag gaattgtaaa tggttctact aattatattc
     3361 ttacaaaaac agttgaaaaa aatatttcat tttcattagc attaaaagaa gcacaacaaa
     3421 aaggctttgc agaaaccaat cctttacttg atgttggtgg gtttgatgcc aaatacaaat
     3481 tgcaaatttt attagctcat gcttttggag taattactca accacaaaac atttttaatt
     3541 taggcatcaa caacctttac gattttgaat taaattatgc aaaagaaaaa ggctacaaaa
     3601 ttaagttggt tgcttatgcc tcaaagaatg aggagggtaa aataatttcg tttgtactac
     3661 caaaatttgt aacaagtaaa gataaattat tttcggtaga tgatgttttt aatggtgtaa
     3721 aaatccaatc atacttctgc gataatcaat tttttgttgg taaaggtgca ggtgcttatc
     3781 caacatcatc agcagtatta tcagacattt cagcattaac ttatgattac cgttatgagt
     3841 ataaaaaact taatactcaa cattcactag caaatgagga agatatattt ttaaaaatat
     3901 ttttttgcgt ttttccttac cagttgattt tgagtttaaa ccatttttta aaaatattga
     3961 agaatactat ttcaatgaaa acaatggctt tataataggc acttgctctc taaaatgtat
     4021 taaagaaatt gttgcaataa gaaatataga aacttcaatc atactttttg atattattaa
     4081 aagagatttc aattaccaaa caattaacaa tcaaaaaaca gaatatgaac ttagttacta
     4141 aaaaaattgt aatggcaaaa gatattggca ttcacggaaa tttatttggt ggaattttaa
     4201 tggcttggat agatgaagca gcagcagctt atgctacaga atattgccac acaccgaata
     4261 tggttactgt acatgtagat gcttttactt ttaaaaaacc tttaaaggca gggcagcata
     4321 ttagaattta tgcttgtgtt aaagagcttg gaagaacttc tatcacacta aaactagaag
     4381 catacaaata caacttgtac agcagagaag aaacacttgt ttgcaatact aatgcaactt
     4441 ttgtaagaat tgatgacgat ggaacaccaa cagcaattgg tgaaacaata agaagaaacc
     4501 attatgaaaa attaccaata acaacgtaac agttttatta attaagtt
//
LOCUS       DAOQPK010000181         4494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_12397_length_4494_cov_4.372606, whole genome shotgun sequence.
ACCESSION   DAOQPK010000181 DAOQPK010000000
VERSION     DAOQPK010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12397_length_4494_cov_4.372606"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="PLU36_08425"
     CDS             <1..1088
                     /locus_tag="PLU36_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HMZ46813.1"
                     /translation="AIIFFPLILTAQNSNQFSINGTIEGLKDSTLVFLNSSNGVAIAQ
                     DYSFKGKFNLKGNLDEPDLHKLAFIGVEQELSIFMYNESVALKGNVNNLAKTTITGSK
                     LHNDYNEYLKVFEPLRLQLNSIATKVNMTQLPKQRDSLLKQFDLTKKNVVTALNKYTS
                     TRLSSPVSSFVLYVVNPVLDGGVDEVEARYNTFKPSAKEGEFARLVEDLIKNHKAKQA
                     AEASTNQGALAPDFTQTDVDGKMVSLSSFRGKYVLVDFWASWCRPCRLENPNVVAAFN
                     KFKDKNFTVLGVSLDKTADAWKNAIKQDNLTWTHVSDLKFWDNAAARLYNVNSIPYNL
                     LIDPNGKIVAKNLRAEELHETLEKILK"
     gene            1184..2167
                     /locus_tag="PLU36_08430"
     CDS             1184..2167
                     /locus_tag="PLU36_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ46814.1"
                     /translation="MSFLKVENLSKVINNKAIVNQVSFKQFKRENIAIAGATGSGKTT
                     LLKLIAGLLQPTNGNVFLEENRVKGSEEKLLPGHKSIAYLNQHFELRNNYFVHELLDV
                     FNAITEEEAYRIYTICAITNLLQRKTNELSGGEKQRIALAAQLVTNPSLLLLDEPFSN
                     LDLWQKNNIKNVLTDLQKELQLSIILTSHDWTDILPWANTILVMKNGEVIQQGNPFTI
                     YHNPINEYVAGLSGAYNIIDVSNVQHFSNSILKQDALKKIFLRPEQIEIEPANNIFQK
                     DVIQEVYFYGSYNIVDVLVNNEIVKVKTTNNTLTVGQRVFLTVKPIEFWYL"
     gene            2242..2367
                     /locus_tag="PLU36_08435"
     CDS             2242..2367
                     /locus_tag="PLU36_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46815.1"
                     /translation="MFGKILINKQAIIFKNVNTIIIDEIGRLNSGTFNVQIMMNN"
     gene            complement(2507..3235)
                     /locus_tag="PLU36_08440"
     CDS             complement(2507..3235)
                     /locus_tag="PLU36_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46816.1"
                     /translation="MKSKLLIFAMYSITLALVFSSCKKDSGDTQYVCTTCNSTPEGLA
                     ANDNLAKGVYKGVVIGSSGTIKFDINNSGNTITATLTLDGQTATLTSQVSVPPSGTYM
                     SPFTGTLNGQPVSVTFSVNFDGTSPTITTSNIPGHPNAVFIIAKETSTVLVRCFEGTY
                     STNEPENGTFNIVVSTILKGWKGKSRETGSTTTKDIDGSYVNNILYFGTGTTTPIATI
                     NGDSFSGSFFDGSYTVTVNGQRKS"
     gene            3419..4063
                     /locus_tag="PLU36_08445"
     CDS             3419..4063
                     /locus_tag="PLU36_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46817.1"
                     /translation="MKTYKVPILLTIIMFVLLNNIQSQKAEIYYGKINTIMGNFEGQF
                     KINLNDENKDMIEIKIAVIKQKKKGEKIIYKEVLKVNAAGIRSFEIDSTIYIVKNIEL
                     ENNKTLQNCCLRITDSCSVATLAYWGTSLENDKCFVLTKNSSYYKTLKSISYFIYFTN
                     FSKCETIKNKNIEEENKWNSDGLNRMKAYEALTQQELLERWKSRITAYKNCFSN"
BASE COUNT         1726 a          577 c          760 g         1431 t
ORIGIN      
        1 ttgcaattat tttttttccg ttaatactaa cagcacaaaa tagtaaccag tttagtatta
       61 atggtacaat agagggatta aaagatagta cattggtttt tttaaattct agtaatggag
      121 tagcaattgc tcaagactat tcatttaaag gaaaatttaa tttaaaagga aatttagacg
      181 aaccggattt gcataaactt gcttttattg gtgttgaaca agaattgagc atctttatgt
      241 ataatgaaag tgtagcatta aaaggtaatg ttaataattt agctaaaaca accattactg
      301 gctctaaatt gcataatgac tacaacgagt atttaaaagt atttgaacca ttaaggttgc
      361 agttaaattc aattgcaaca aaagtaaata tgacccaatt accaaaacaa agagactcgt
      421 tattgaagca gttcgattta accaaaaaaa atgtggtaac tgcattgaat aaatatacaa
      481 gtacaagatt atcttctcca gtaagttcct ttgttttgta tgtagtaaac ccagttttag
      541 atggtggtgt ggatgaggtt gaagcaagat acaatacatt taaaccatcc gcaaaggaag
      601 gtgagtttgc aagactagtt gaagatttaa ttaaaaatca taaggccaaa caagctgcag
      661 aagcatctac caatcaaggg gcacttgcac cagattttac acaaactgat gtggatggga
      721 agatggttag cttgtcttct tttagaggta agtatgtttt agtagatttt tgggctagtt
      781 ggtgcaggcc atgtagatta gaaaatccta atgtggttgc agcattcaat aaatttaaag
      841 ataaaaattt taccgtttta ggtgtttcat tagataaaac tgctgatgca tggaaaaatg
      901 caattaagca agacaactta acttggacac atgttagtga tttaaaattt tgggataatg
      961 ctgctgcaag attgtataat gttaacagca ttccatataa tttactaatt gaccctaatg
     1021 gtaaaattgt ggctaaaaac ttaagagccg aagaactgca tgaaacctta gaaaaaatat
     1081 taaaataact tttggttata gtaatagaaa atgctgtctg ttttttggca gcattttttt
     1141 tattaaataa aagctacatt ataagtaagt ttatttttga tttatgagtt ttttaaaagt
     1201 agaaaattta agtaaggtaa taaataataa agcaattgta aaccaagtta gttttaagca
     1261 atttaaaaga gaaaatattg caattgcagg agctacaggc tcggggaaaa caaccttatt
     1321 aaaattaatt gctggtttat tacaacctac caacggaaat gtgtttttgg aagagaatag
     1381 ggtaaaaggc tcagaggaaa aattattacc agggcataaa agcattgcat atttaaacca
     1441 acattttgag ttaagaaata attattttgt acatgaactg ttagatgttt ttaatgcaat
     1501 aacagaagag gaggcttata gaatttatac tatttgtgca attaccaatt tattacaaag
     1561 aaaaaccaat gaactatctg gtggagaaaa acaacgaatt gccttagctg cacaactggt
     1621 tacaaaccca tcattgctat tgttagatga gcctttttct aatttagatt tatggcaaaa
     1681 aaataatata aaaaacgttt taacagacct tcaaaaagaa ttacaattaa gtattatcct
     1741 aacatcgcat gattggacag atattttgcc ttgggcaaac acaattttgg taatgaagaa
     1801 tggagaagta atacaacagg gaaatccttt tactatttat cacaatccaa ttaatgaata
     1861 tgtggcagga ttaagtggag catataatat tattgacgta tccaatgttc agcattttag
     1921 taattcaatt cttaaacaag atgctttaaa gaaaatattt ttacgaccag aacaaataga
     1981 aatagaacct gctaataata tttttcaaaa agatgtaatt caggaagttt atttttatgg
     2041 aagttacaac attgtagatg ttttagtaaa taatgaaatt gtaaaagtta aaacaactaa
     2101 taatacttta actgttgggc aaagggtatt tttaacagta aagccaattg aattttggta
     2161 tttatgatgt agaatgtaaa atgaaatttt attttattac aactgaaaaa agaggaagca
     2221 gttttcatta gagtaataga tatgtttggt aaaatattaa taaataaaca agccataatt
     2281 tttaaaaatg taaatacaat tattatagac gaaattggta gattaaactc tggtacattt
     2341 aatgtacaaa taatgatgaa taattaaatg ttaactgaga agttgattgt aacaaaataa
     2401 taatccctat gttttcgaaa aagagagagg ctgcacaaat gcagcctctc tcttttgaat
     2461 aataaagaac ataaatatta ggaaaaagta aaaaggaagt aactaattac gattttcttt
     2521 gcccatttac tgttacagta tagcttccat caaaaaaaga acctgaaaaa gaatctccgt
     2581 taatagttgc tattggagta gttgtgccag taccaaaata caaaatattg tttacatagc
     2641 taccatcaat atctttggta gtggtactac cagtttctct tgattttcct ttccagcctt
     2701 ttaaaatggt agatactact atattaaaag ttccattctc tggttcattg gttgaataag
     2761 ttccttcaaa gcatcttacc aaaactgttg aagtttcttt tgcaattata aaaactgcat
     2821 taggatgtcc tggaatattg gatgtagtaa tggtgggtga tgtgccatca aagtttacag
     2881 aaaaagtaac cgaaacaggt tgtccattta atgtaccagt aaatggagac atataagttc
     2941 cagatggtgg tacagatact tgagaagtta aagtagcagt ttgcccatct aatgttaaag
     3001 tagcggtaat agtgtttccc gaattgttta tatcaaactt tatggtacca ctcgacccta
     3061 taacaactcc tttataaaca ccttttgcaa gattatcgtt tgctgccaat ccttctggtg
     3121 tactgttgca tgtagtacat acatattgtg tatcaccact atctttttta caagatgaaa
     3181 acacaagtgc caatgtaatt gaatacattg caaaaatcaa aagttttgat ttcataaatg
     3241 tggttttaat tgtttttaat tagaatatag caactactac attctaaaag taaatctatg
     3301 tactaatctt gaaatcctta atacccacca gaaggtattt taaaaattat caataacctc
     3361 ttaaaataaa atagatattt ttactaacaa tcataaacac tttgttatat aaaaaatcat
     3421 gaaaacgtat aaagtaccaa tacttcttac aattattatg tttgttttat taaacaatat
     3481 tcaatctcaa aaagcagaga tttattatgg aaaaataaac acaatcatgg gcaattttga
     3541 aggacaattt aaaattaatt tgaacgatga aaataaggat atgattgaaa taaaaattgc
     3601 cgttattaaa caaaagaaaa aaggcgaaaa aattatttac aaagaagtat taaaagtaaa
     3661 tgcagcagga attagaagtt ttgaaataga tagcacaatt tatattgtaa aaaatataga
     3721 gttggagaat aacaaaacgc ttcaaaattg ttgtttaaga ataacagata gttgtagtgt
     3781 agctacttta gcttactggg gaacaagttt agaaaatgat aagtgttttg tattaactaa
     3841 aaatagtagt tattataaaa ctttgaaatc aataagttat tttatttatt tcaccaattt
     3901 tagtaaatgt gaaactataa aaaataaaaa cattgaagaa gagaataaat ggaattctga
     3961 tggcttgaat agaatgaaag cttatgaagc attgacacaa caagaattat tggaaaggtg
     4021 gaaaagtaga ataacagcgt ataaaaattg ttttagtaat tagtaaacta tttgtttaaa
     4081 atttgctgta ctgttactaa taaaattaaa caaaaaaggt ttgtcattct gaatgaaatg
     4141 aagaatctga atgtactttg gaacaaacca ctagtagatc cttcgctacg ctcaggatga
     4201 caaaaatggg atggttcaga atgacaatgc acacttactg tcatgttgag gcaacgaagc
     4261 atctgtaaag ttaaggatag agattcctcg caaactcgga atgacataga attaaagatc
     4321 cttcgcttcg ctcaggatga caatctatga gtgctcagga tgacataaat gggagagttc
     4381 tgaatgacaa tgtacactta ctgtcatgct gaggcaacga agcatctgta aagttaagga
     4441 tagagattcc tcgcaaactc ggaatgacat agaattaaag atccttcgct acgc
//
LOCUS       DAOQPK010000182         4464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_12534_length_4464_cov_3.998412, whole genome shotgun sequence.
ACCESSION   DAOQPK010000182 DAOQPK010000000
VERSION     DAOQPK010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12534_length_4464_cov_3.998412"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PLU36_08450"
     CDS             complement(<1..270)
                     /locus_tag="PLU36_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46818.1"
                     /translation="MFSLLLLLKQLPTKQIASNTFTLLFKIFEKHGLPRILYVDRAGV
                     YGGAKRTNFSQLVHALEALGIEILYANSPQGKRRVERLFRTLQDRL"
     gene            417..917
                     /locus_tag="PLU36_08455"
     CDS             417..917
                     /locus_tag="PLU36_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46819.1"
                     /translation="MKKIFIIALAALVGFTANAQEKKESSSSKGFSFGAGPALSMPIG
                     SFGTTHSFGYGVELQGTYHASESFEAFAQLGWSNFSIKSAYGGGSQSLIPVLVGGRYV
                     SGGFTAGAGIGFGSFSKGGGSGFAYSPQIGYNFDKFNLNLHYTALSAKGGGSIGYIGL
                     KGYYNF"
     gene            1059..1493
                     /locus_tag="PLU36_08460"
     CDS             1059..1493
                     /locus_tag="PLU36_08460"
                     /inference="COORDINATES: protein motif:HMM:NF014346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMZ46820.1"
                     /translation="MLKNNKKKLIIVSLLSCIISVSIFSFTNKQQGPVKFKNLKVLPQ
                     DISDEDLDKVMDGFKAALGVKCGFCHAPSKENPKKLDMASDDNPRKDITREMMRMTQE
                     MNMKYISKIPHADTVKLQMLTCNTCHRGATKPFGEPLVKPKQ"
     gene            complement(1550..2020)
                     /locus_tag="PLU36_08465"
     CDS             complement(1550..2020)
                     /locus_tag="PLU36_08465"
                     /inference="COORDINATES: protein motif:HMM:NF025675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HMZ46821.1"
                     /translation="MQFGFNYNKKKTIQALRFHFISRKEIRLLVIVVNVFAIVSAILF
                     YSKKIRPEYFLLGSILWLAIMIASWYILPYTVYKKTEMFKHYFIVTLQQKGIVLESEK
                     GEIEWSWDKFQSYTESPNFFHLYFNPKSFLLIPSDEIGDEMRHEIRGFLRQIKK"
     gene            complement(2523..3587)
                     /gene="prfA"
                     /locus_tag="PLU36_08470"
     CDS             complement(2523..3587)
                     /gene="prfA"
                     /locus_tag="PLU36_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HMZ46822.1"
                     /translation="MIDKLDAIKARFDQIGVALTNPEIINNQKEFGKLSKEYRSLEKI
                     VQPFELYKKVLTDHAFAKESLNSNDEDLKELAKMELPELEEQKETLEKQLTQLLIPKD
                     PQDDKNAILEIRAGTGGDEASLFAGDLLGMYLRYCQKKGWKASVVSESEGTVGGYKEV
                     VVEVTGEDVYGTLKFESGVHRVQRVPNTETQGRVHTSAATVAVMPEAEEVDVELKESD
                     VKMETARSGGAGGQNVNKVETKVFLTHLPTGIVVVCQTERTQLANREKAMTMLRSKLY
                     EEQVRKQEDEIARQRKTLVSTGDRSAKIRTYNWPQGRVTDHRIGLTSYNLDAVINGEI
                     DEFIENLQLAENAEKMANNG"
     gene            3718..4197
                     /locus_tag="PLU36_08475"
     CDS             3718..4197
                     /locus_tag="PLU36_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46823.1"
                     /translation="MKKTFIIALFVTLGLTVNAQTEKSTWLLGGDISLSINSPKGGGS
                     NTVFTLSPDAGYFFMKNYAIGGMFNLTTGGGQTSWMLAPFIRGYFSNNKTGKPFAQVG
                     LGVGGAGGGTTTSFVLKGGYAMFLNKSVALEMGGQLALQTGLTQFALGAGFQIHFAK"
     gene            complement(4248..>4464)
                     /locus_tag="PLU36_08480"
     CDS             complement(4248..>4464)
                     /locus_tag="PLU36_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46824.1"
                     /translation="EIPEEQNIKPMIQDLGDKIKSLNTPKLQLSIFDTQTQVFEDIRK
                     TLEGIDINRLTPVEALMKLNEIKGMIK"
BASE COUNT         1410 a          726 c          737 g         1591 t
ORIGIN      
        1 taacctgtcc tgaagggtcc tgaagagcct ctcaacgcgt cgtttgcctt gaggggagtt
       61 agcatagagg atttcaatac caagcgcctc tagggcgtgt acaagctgag aaaaattggt
      121 tctcttggct ccgccataaa ctccagctcg atcaacatat aatatcctgg gtagaccatg
      181 tttttcgaaa attttaaaaa gaagtgtaaa agtattactt gcaatttgtt ttgttggtaa
      241 ttgtttcaat aataataaaa gagaaaacaa aatatttatt tatagtgtta ataaaattta
      301 tacatctgtt tcttttatgc acatatttta aaataccctt aagtgggtat tgaattaagt
      361 attaattact ttaccttcgg ctttattatt taaccctttt ataaaatttt aacaacatga
      421 aaaaaatttt tattattgct ttagctgcct tagtaggctt tacggctaat gcacaagaaa
      481 aaaaagagtc ttcatctagc aaaggtttta gctttggtgc tggccctgct ttatctatgc
      541 caattggtag ctttggtaca acacatagtt ttggctatgg tgtagaatta caaggtactt
      601 accatgcatc tgaaagtttt gaagcatttg cacaattagg ttggagcaac ttctctatca
      661 aatctgctta tggtggtgga agccaaagtt taattccagt tttagttggt ggtcgttatg
      721 tatctggtgg ttttactgct ggtgctggaa tagggtttgg ttctttctct aaaggcggtg
      781 gtagcggctt tgcttatagc ccacaaattg gatacaattt tgacaaattt aacttaaacc
      841 tacactatac tgcattatct gctaaaggtg gaggttctat aggttatatt ggtcttaaag
      901 gatattataa cttttagtat ttactaaggt taaatagtaa aaaaccactt cgtaaagaag
      961 tggtttttta ttgtaagaca tttttttatt tgaaataatg ctataaattt tttgaaataa
     1021 tttataaata agtatattta gtaactaaat ttatttgtat gttaaaaaac aataagaaaa
     1081 aattaattat tgtaagcttg ttatcgtgta ttatttcagt atcaattttt tcatttacta
     1141 ataagcaaca aggtccagta aaatttaaaa atttaaaagt attaccacag gatatttctg
     1201 atgaagattt agataaggta atggatgggt ttaaggctgc cttgggtgtt aaatgtggtt
     1261 tttgccatgc tccatcaaaa gagaatccta aaaaattaga tatggcaagc gatgataatc
     1321 caagaaaaga tattacaaga gaaatgatga gaatgacaca ggaaatgaat atgaagtaca
     1381 tttctaaaat accacatgcc gatactgtta aacttcaaat gcttacttgc aatacttgcc
     1441 acagaggggc aacaaaacct tttggcgaac cacttgtaaa gccaaagcag taaagattgt
     1501 atttaataat taccgaagcc ttattatttg aaaaaagtag taaggctttt tattttttta
     1561 tttgccttaa aaagcccctt atttcatgcc tcatttcatc gcctatttca tcagaaggga
     1621 ttaataggaa ggattttggg ttgaaatata aatgaaaaaa attagggctt tcggtataac
     1681 tctgaaattt atcccaactc cattctattt ctcctttttc tgattctaat acaatgccct
     1741 tttgctgcaa tgtaacaata aaataatgtt taaacatttc agttttttta taaacagtat
     1801 aaggcaaaat ataccaacta gcaatcataa tagctagcca taaaatagag cctaataaaa
     1861 aatactcagg acgaattttt ttagaataaa ataaaatagc agatacaatt gcaaatacat
     1921 tcacaacaat aaccaaaagt ctaatttctt ttctagaaat aaaatgaaat cgtaaagctt
     1981 gaattgtttt ctttttatta taattaaaac caaattgcat aattattagt ttacgttact
     2041 ttgaacaaag taaaggagaa agtgtgtcat tctgagcgta agtgaaacaa aaaagccatg
     2101 tcatactgag cgtcagcgaa gaatctttaa ttctatgtca ttccgagcct gcgaagaatc
     2161 tctttgtatt aaacctttag atgcttcttt gcctcagcat gacaagaaaa ggtttgtcat
     2221 tccgagttta cgaggaatct cattgttctt aacctttaga tccttcgcta cgctcaggat
     2281 gacacgaaaa gggatcagga tgaaataaaa tagggctcag gataaaatat agtgaataaa
     2341 gtgaattaaa aaatgcttgt cattccgaat ttacgaggaa tctctttgtt cttaaccttt
     2401 agatccttcg ctacgctcag gatgacaaaa aaaggggagc aggatgacaa gaaaaggggc
     2461 tcagaatgac ataaaggaaa atcagaataa aatgaaattg gttttttgca actcctaatt
     2521 atttacccat tattagccat tttttcggca ttttctgcta attgcaaatt ttcaataaac
     2581 tcatctatct ctccatttat tacagcatct aaattgtaag atgtaagtcc tattctatgg
     2641 tctgttaccc tgccttgagg ccaattataa gttctaattt ttgcacttct atcaccagta
     2701 cttactaagg tttttctttg tctggcaatt tcatcttctt gtttgcgtac ctgctcttcg
     2761 tataatttac ttcttaacat agtcattgct ttttctctgt ttgccaactg cgttctttct
     2821 gtttggcaaa ccacaacaat acctgtaggt aagtgggtaa gaaatacttt tgtttctact
     2881 ttgtttacat tttgtcctcc agcaccacca ctacgtgcgg tttccatttt tacatcactt
     2941 tcttttaatt caacatcaac ctcttctgcc tctggcataa cagcaactgt ggcagcactt
     3001 gtatgaactc tgccctgagt ttctgtatta ggtacacgtt gtacacgatg cactccactt
     3061 tcaaatttta aagtaccata aacatcttcg cctgtaactt ctacaacaac ttctttataa
     3121 ccaccaacag ttccttcgct ttcgcttact acacttgcct tccagccctt tttttggcaa
     3181 taacgcaaat acatacctaa taaatctcct gcaaacaagc ttgcctcatc tcctccagta
     3241 cctgcacgaa tttctagtat agcattttta tcatcttgtg ggtctttagg aattaataat
     3301 tgagttaatt gtttttctaa ggtttctttt tgttcttcta attcgggtaa ttccattttg
     3361 gcaagttctt tcaaatcttc gtcgttacta tttaaacttt ctttggcaaa agcatggtcg
     3421 gttaatactt ttttatataa ctcaaatggt tgtacaattt tttcgagaga acgatattct
     3481 ttacttagtt taccaaactc tttttggtta ttaataattt cagggttggt taatgctaca
     3541 ccaatttggt cgaaccttgc tttaattgca tctaacttat caatcatgcc actttatttt
     3601 tgggagtgca aaagtaatac attggcttgt ttaagttgga gataaaataa gtagattttt
     3661 tttaaatata aaggaatcat gtaattttac ttattaattt aaccaaaaac gaatattatg
     3721 aaaaaaacgt tcattattgc attatttgtt actttagggt taacagtaaa tgcacaaacc
     3781 gaaaaaagta catggttatt aggaggcgat atttcacttt caattaattc accaaagggc
     3841 ggaggttcca atactgtttt tactttatct ccagatgctg gttatttctt tatgaaaaat
     3901 tatgcaattg gcggtatgtt taacttaaca acaggtggtg gacaaactag ttggatgttg
     3961 gctcctttta ttagaggcta tttttcaaat aataaaactg gtaaaccatt tgcacaagtt
     4021 ggattaggtg ttggaggagc aggtggtgga acaacaacta gctttgtttt aaagggtgga
     4081 tatgcaatgt ttttaaataa aagtgttgct ttagaaatgg gcggccaact ggctttacaa
     4141 acagggttaa cacagtttgc acttggggct ggctttcaaa tacattttgc aaaataattt
     4201 tgaaaaagct taagaataaa ataactactc ataacagggt agttatttta ttttatcatc
     4261 cctttaattt cgtttaactt cattaatgct tctactggtg ttagcctgtt aatatctata
     4321 ccttctaatg tttttctaat atcttcaaaa acctgtgttt gtgtatcaaa aatagagagt
     4381 tgtaatttag gagtgtttaa agattttatc ttatctccta gatcttgtat cattggtttt
     4441 atattttgct cctctggtat ttct
//
LOCUS       DAOQPK010000183         4451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_12598_length_4451_cov_4.741356, whole genome shotgun sequence.
ACCESSION   DAOQPK010000183 DAOQPK010000000
VERSION     DAOQPK010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12598_length_4451_cov_4.741356"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PLU36_08485"
     CDS             <1..505
                     /locus_tag="PLU36_08485"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HMZ46825.1"
                     /translation="FHPLLNDSKQVKEKDRNELRWYIEQNALAYAVSKIDNNEIDKIN
                     ILKASFKAMHLALDNLKLKPDLLLIDGNRFLQYKKIKHHCIVKGDGKFASIAAASILA
                     KTYRDEFMQQLHLEFPVYGWNKNKGYGTLQHRKAIEQFGLSKYHRKSFNIVPKQIELF
                     KKVSNDF"
     gene            495..1130
                     /gene="pnuC"
                     /locus_tag="PLU36_08490"
     CDS             495..1130
                     /gene="pnuC"
                     /locus_tag="PLU36_08490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HMZ46826.1"
                     /translation="MTFEIFTQQLVSGVQQTQWYELVAVFAGIASVWFSRKENILVYP
                     VGLINTIMYVYISFKHHLLGEATVNFYYTIMSIYGWWLWTRKDKQSHEIVLHISYSTK
                     KEWIEQLCFFAVVFAICFTALHLAKQYFFEGVIPAADAFATATAFTGMWLMAKKKVES
                     WWWWIATNITSIPLYFVKSLVFTSVQYIVLLVLAIAGLITWMKKAKAENKY"
     gene            complement(1144..2265)
                     /gene="hemW"
                     /locus_tag="PLU36_08495"
     CDS             complement(1144..2265)
                     /gene="hemW"
                     /locus_tag="PLU36_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HMZ46827.1"
                     /translation="MAGIYIHIPFCKQACHYCNFHFSTLHNNMPEMLDAIIKEASLQK
                     NYLQEAIETIYLGGGTPSIYKPSDIEKIITHLHQTFTVIPNVEITLEANPDDITEEKL
                     QQWKNIGINRLSIGIQSFVEEDLKWMNRAHNSFQALHCLQIATKYFNNITVDLIYGTP
                     YLTNEQWKKNIDTVVQFNIPHISCYALTVEPKTVLHTLIQKNKLPNIDIEKQAQHFEI
                     LTNTLAKAHYEHYEISNFATSGFRSKHNSSYWQGKHYLGFGPSAHSFNGISRQWNISN
                     NALYFKSIAQNKVLYEIEILTTKQQLNEYIMTALRTIEGISLQHVKENFSEEEKEKLL
                     SRSKKLITQQLLSIENNFLKLTPQAKFLADGIAADLFVD"
     gene            complement(2277..>4451)
                     /locus_tag="PLU36_08500"
     CDS             complement(2277..>4451)
                     /locus_tag="PLU36_08500"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HMZ46828.1"
                     /translation="QFRQKFGDDGNTMVFGIESNQFFTPSIFNEVANFHKNLKQIPGV
                     TNVLSIPEAVTLVKDSVGEKLLPQKIFNAPYTTQENLDIDKTLFLSLPFYNSLLYNAN
                     TNAYIIGVQLNKDSVNSKSRTELINNVMAQAKTFEKNTNISLHTSGLPFIRTTIGNRI
                     KDEMNWFLIGSLVLSAITLMLFFRSLSATVMSLLVVLFGVVWSLGTIVLLGYKITLLT
                     ALIPPLIVVIGIPNCIYFLNKYHSTFKETGNKNHALVTMVGRMGVVTLFCNIAAAIGF
                     AVFAFTKSNLLIEFGIVSGINIMALFIISLIFIPIVLSYLPEPKPKHVKYLDNKILEK
                     LLVKIERWSLNNAKWIYTLTIIFCTLAIIGFLKLKSEGFIVDDLPKQDKIYTDLKWFE
                     KNMKGVMPLEIMVDTKKKNGLLRNMNPINKIEELSTYIASKEEAARPMSFVEGLKFAK
                     QAYYDGDSLSYAVPYEGDMAFLGPYLKSNNSSDTVGAKKQNTTSLLNGFMDSTRQTAR
                     ISVNTKDIGNVQLPLLLKDLEKKAAEIFDTSAYKITFTGTSVTFLEGSSFIISGLKES
                     ILYAFLLIAFCMLYLFKSVRILFCSLIPNIVPLLLTAGLMGWAGIRLTPATVLIFSVA
                     LGIVIDVTIRFLVNYKQELPHYNNQTIPTLIQTIKHTGISIIYTSLVLIAGFIIFSFS
                     GFNSTQSLGWLTSFTLVTGTITNLLLLPVLLISLQKKSKEKS"
BASE COUNT         1507 a          634 c          666 g         1644 t
ORIGIN      
        1 ttttcaccct ctattaaatg atagcaaaca agtaaaagaa aaagatagaa atgaattgcg
       61 atggtatatt gagcaaaatg ctttggctta tgctgtaagt aagattgata ataatgaaat
      121 agataagatt aatattttaa aagcaagttt taaagccatg catcttgcat tagataattt
      181 aaaattaaag ccagatttgt tactgattga tggcaataga tttttgcagt ataaaaaaat
      241 taaacatcat tgcattgtta aaggcgatgg taaatttgct tctattgctg ctgcaagtat
      301 tttagcaaaa acatacagag atgagtttat gcaacaacta caccttgaat ttcctgttta
      361 tggctggaat aaaaataaag gctatggcac tttgcaacac agaaaagcca ttgaacaatt
      421 tggactaagt aaataccaca gaaagagttt taatattgta cccaagcaaa tagaattatt
      481 taaaaaagta agcaatgact tttgaaatat ttacccaaca attagtttca ggagtacagc
      541 aaacacaatg gtacgagtta gttgcagttt ttgcaggtat tgcaagtgtt tggtttagca
      601 gaaaagaaaa tattttagtg tatcctgttg gattaattaa cactattatg tatgtgtaca
      661 ttagttttaa gcaccatttg ttaggcgagg caactgttaa tttttattat accattatga
      721 gcatttatgg ctggtggctt tggactagaa aagataaaca aagccatgaa attgttttgc
      781 atatttcgta cagtactaaa aaagaatgga tagaacaact ttgttttttt gctgttgttt
      841 ttgcaatatg ttttactgca ttacatttag ctaaacaata cttttttgaa ggtgttattc
      901 ctgctgcaga tgcttttgca actgctacag cttttacagg tatgtggttg atggctaaaa
      961 agaaagtaga aagttggtgg tggtggatag ctaccaatat tacatctata ccattatatt
     1021 ttgtaaaatc attagtattt actagtgttc agtatatagt attgcttgta ttggctattg
     1081 caggcttaat tacttggatg aagaaagcaa aggctgaaaa taaatactaa gtatgtgtta
     1141 tttctaatct acaaacaaat ctgcagcaat accatctgct aaaaattttg cttgcggagt
     1201 aagttttaaa aaattatttt ctatacttaa taattgttgg gtaattaatt ttttactcct
     1261 gcttaataat ttttcttttt cttcttcgct aaaattttct ttaacatgtt gtaacgatat
     1321 tccttctatt gttcgtaaag cggtcataat atattcgttc aactgttgct ttgttgttaa
     1381 tatttctatt tcataaagaa ctttattttg agctatactt ttaaagtaca aagcattgtt
     1441 acttatattc cattgccttg aaatgccatt aaatgaatgt gcagatggtc cgaaacctaa
     1501 gtaatgtttt ccttgccaat aactgctgtt gtgcttactt ctaaaaccac ttgttgcaaa
     1561 attacttatt tcgtaatgct cataatgtgc tttagctaag gtattggtta atatttcaaa
     1621 atgttgtgct tgtttttcta tatcaatatt gggtaattta tttttttgaa ttaatgtatg
     1681 taaaactgtt ttaggctcta ctgtaagtgc ataacaggaa atatgtggaa tattaaattg
     1741 tacaactgta tcaatgtttt ttttccattg ttcattggtt aaatatggag ttccataaat
     1801 taaatcaaca gtaatattgt tgaaatattt tgtggctatt tgtaaacagt gtagtgcttg
     1861 aaaactgttg tgtgctctat tcatccattt taaatcttct tctacaaaac tttgaatacc
     1921 tatactcaat ctgtttatgc caatgttttt ccattgttgt aatttttctt cagtaatatc
     1981 atctggattt gcttctaagg ttatttcaac attgggtatt actgtaaatg tttgatgtaa
     2041 atgagtaatt attttttcaa tatcagaagg tttataaata cttggtgtgc cacctcctaa
     2101 ataaattgtt tcaatagctt cctgaagata atttttttgt aatgatgctt cttttataat
     2161 agcatcaagc atttcaggca tattgttgtg taatgtgcta aaatgaaagt tgcaataatg
     2221 acaggcttgt ttgcaaaaag gaatatgtat gtaaatgcct gccatagctt aaaaaattag
     2281 cttttctctt ttgatttttt ttgtaatgaa attaataata caggtaataa taataaatta
     2341 gttatggtac cagtaaccaa tgtaaaagaa gttagccaac ctaaagattg agtactatta
     2401 aaaccactaa aactaaaaat aataaaacct gcaattaata ccaatgatgt atagataatg
     2461 cttatgcctg tgtgtttaat agtttgtata agtgttggta tggtttggtt attatagtgt
     2521 ggtaattctt gtttgtagtt aactaaaaaa cgaatggtta cgtctatcac aatacctaaa
     2581 gcaacactaa aaattaaaac agttgcaggt gttaacctta taccagccca acccattaag
     2641 cctgcagtta ataataatgg aacaatgttt ggaattaaag aacaaaataa aattctaaca
     2701 gatttaaaaa ggtatagcat acaaaatgca attaataaaa atgcgtaaag gatactttct
     2761 tttaaaccag aaattataaa actactacct tctaaaaatg taacacttgt accagtaaac
     2821 gtaattttat aagcagatgt atcaaaaatt tcggctgctt ttttttctaa atctttaagt
     2881 aataaaggca attgaacgtt tccaatgtct tttgtgttaa cacttattct tgcagtttgc
     2941 cttgtactat ccataaaacc attaagcaaa ctagtagtat tttgtttttt tgcaccaacc
     3001 gtatcgcttg aattattaga ttttaaataa ggccccaaaa aagccatatc accttcgtaa
     3061 ggtactgcat agcttaaact gtctccatca taatatgctt gctttgcaaa ttttaatccc
     3121 tctacaaaac tcataggtct tgctgcttct tctttacttg caatgtaagt agatagttcc
     3181 tcaattttat ttattgggtt catgttgcgt aataacccat ttttcttttt ggtatcaacc
     3241 attatttcta atggcattac acctttcatg tttttttcaa accattttaa gtcggtataa
     3301 attttatctt gtttaggtaa atcatctact ataaatcctt cgctttttaa ttttaaaaaa
     3361 cctataatag ctaaagtgca aaaaattatt gttaaggtat aaatccactt tgcattgttt
     3421 aatgaccaac gttctatttt tactaatagt ttttctaaaa ttttattatc taaatattta
     3481 acatgttttg gtttaggttc tggcaaataa cttaaaacaa taggaataaa aattaatgaa
     3541 ataataaata aagccataat gtttatgcct gatacaatac caaactcaat taataaatta
     3601 ctttttgtaa atgcaaatac tgcaaaacca atagctgcgg caatattgca aaaaagtgta
     3661 acaacaccca tacgcccaac catagtaact aatgcatgat ttttattgcc tgtttcttta
     3721 aatgtagaat ggtatttgtt taaaaagtag atacaattag gaatgccaat tacaacaatt
     3781 aaaggtggta ttaaagcagt taacaatgtt attttgtaac ctaataatac tattgttccc
     3841 aagctccaaa caacaccaaa aagtacaacc agtaaactca ttactgtagc acttaaactt
     3901 ctaaaaaaga gcattaaggt aatagctgat aaaactaatg agcctattaa aaaccaattc
     3961 atttcatctt taattctatt accaattgtt gttcttataa aaggcaagcc actagtatgc
     4021 aaactaatat ttgtattttt ttcgaatgtt ttagcttgtg ccattacatt gtttatcaac
     4081 tctgttctac ttttactatt tacgctgtct ttgtttagct gaacaccaat tatataagca
     4141 tttgtattag cattatataa aagtgaatta taaaaaggca aggataaaaa caaggtttta
     4201 tctatatcta aattttcttg agttgtgtac ggggcattaa atattttttg gggtaataat
     4261 ttttctccaa ccgaatcttt tactaaggtt actgcttcgg gtatgcttaa aacatttgta
     4321 acaccaggta tttgttttaa gtttttatga aaatttgcta cctcattaaa tattgaaggt
     4381 gtgaaaaatt ggttgctttc aattccaaaa accattgtgt tgccatcatc tccaaatttt
     4441 tgtctgaact g
//
LOCUS       DAOQPK010000184         4421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_12739_length_4421_cov_3.390518, whole genome shotgun sequence.
ACCESSION   DAOQPK010000184 DAOQPK010000000
VERSION     DAOQPK010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12739_length_4421_cov_3.390518"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PLU36_08505"
     CDS             complement(<1..378)
                     /locus_tag="PLU36_08505"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009231 - riboflavin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HMZ46829.1"
                     /translation="MLHTIAEAIEDIKNGKMIIVVDDEDRENEGDFIIAAHYATPEVI
                     NFMSKEGRGLICVALTEQRCLELDLQPMVNSNTSLHETAFTISVDLVSNETTTGISAQ
                     DRSKTIQALINSATKASDLGRPGH"
     gene            600..3881
                     /locus_tag="PLU36_08510"
     CDS             600..3881
                     /locus_tag="PLU36_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMZ46830.1"
                     /translation="MLKKHLLQFVLALFMLPAIVVAQNTTSTLSGSVKTTTGEALIGA
                     TVIATHEPTGTVYKVQARSGGRYDISNMNPGGPYSIVVSFVNFQNEKKSDIYLGLGET
                     YKIDFILVAKATDLGEVTVKTTSKKTTDFTSKGGAETFITKEKMDNLPTVGRNLQDYL
                     RFVPQAKVASSDGNLAGVSFGGQNNRYNSFYVDGAANNDQFGLASSGTNGGQTGSSPI
                     SIDAIDQIQIILSPYDASIGNFTGAGINATTKSGTNKLQASVYTFFRNQDLTGKTPNG
                     DKKLATKLAKFSSKTYGFRVGGPIVKNKAFFFLNVDLVRDDRPQPWTGTYAGTTPLNS
                     PNFTALMTKLSGYGYDPGGYENNVEEVNSNRLAAKIDWNLNTTHKLTLSYRYSKSDRF
                     NVPTSSNTSINFYNGGYLMPNVTHSGSAELRSIFKKGATNKLLLTFTDVTDDRGPLGK
                     AFPRVTINDGSGRFVFGTENFSTANLLTQKNFAFLDYFKFIKGNHSFTIGTDNEYTKA
                     RNVFIRDNYGNYTFANLNDFLNNAYPSVYSRSFSLLDNKTGDATASAAKFSFARLAGF
                     VNDEISMGDNFTLNVGLRLDYTTFLYSPAKDPYFNDIALPKIAAHYDLQGARSGKISN
                     PKASLSPRIGFTYKIPEENVVFRGGAGLFAGRIPLVWPGGVYNNTGLSVGGVSLNSIT
                     ATTAANLHFIADPYNQPTAQSLGINISNAKGQVDLIAKDFRLPKVFRASLAFDKRFDN
                     GWSLTAEVILTKNINEIYYQNLNILPPTLKATGPDNRTVYAAGNASAPYIPINNNNTN
                     PYTGIYLISNKKDKKGFAHNFTVTANKNFTNGLSFNASYAYGYSEVTYEGTSSQNNSQ
                     YNTLATVNGRNFADRHRSNFDLGHRITAFLSKKFTYARKSMSTTVSLFYTGQSGNPLT
                     YVYNGSMVRDVSTANSQDLIYIPTAADITNAVFVTSGGLTPAQQKAALETFISGNKYL
                     SSRRGQYAENNGDRLPFTNVLDLSIKQDFNLKIGKNKYQIQVSYDIFNFSNMLNRDWG
                     RQYFVNFNTYSLMSFRGYQPNTTIPTYSFVPPASGSVHTVSTSTAPSFSARWVSQLGV
                     RLSLF"
     gene            complement(3961..>4421)
                     /locus_tag="PLU36_08515"
     CDS             complement(3961..>4421)
                     /locus_tag="PLU36_08515"
                     /inference="COORDINATES: protein motif:HMM:NF040120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HMZ46831.1"
                     /translation="FNVIGMLTFTNRVENSFTINDKAGNRSGCAALFFTFHYGLFHFA
                     YMFFLISYIKDITPSQFTFIKLSFWAILAGSIIQYLQDKARNRTQPVNIGNMFVLPYA
                     RIVPMHLVILLPKFLSISPSIVFVVLKLFADLIMHIAYKNFVFKKSNQSL"
BASE COUNT         1535 a          714 c          711 g         1461 t
ORIGIN      
        1 atgcccaggt cttcctaaat ctgatgcttt tgttgctgaa ttaattaaag cctgtatagt
       61 ttttgaacga tcttgagcag aaatgccagt agtagtttcg ttacttacta aatctaccga
      121 aatggtgaat gcagtttcgt gtaaagaggt attagaatta accattggct gtaaatcaag
      181 ttccaaacac ctttgctctg ttagggcaac acaaattaag cctctaccct ctttgctcat
      241 aaaattaatc acttctggtg tagcatagtg tgcagcaata ataaaatccc cttcattttc
      301 tctgtcttca tcatccacaa caataatcat tttgccattt ttaatatctt ctatggcttc
      361 ggcaatagta tgtaacatgt tttaacctta aatcaattta aaagaatgca aatgtacgcc
      421 taaaccttgt ttggctaaaa tgcatctaaa ttttttactt aaacatgcta aaaattaaaa
      481 aaagaaaaaa aattgcacag tttatgcaca aaaacataaa aaaaataaaa atttgttaac
      541 gcattgttac agattattat actttattca ctttctttgc aaaaacaaat atcaaaatta
      601 tgcttaaaaa acatttacta caatttgtat tggcattgtt tatgttgcca gcaattgttg
      661 tagcacaaaa cacaaccagt acattaagtg gttcggtaaa aaccactact ggagaggcat
      721 taattggtgc tactgttatt gcaacgcatg aaccaactgg aactgtatat aaagtacagg
      781 ctcgttctgg tggtcgttac gacatcagta acatgaaccc tggaggccct tatagtattg
      841 ttgtttcttt tgtgaatttt caaaatgaga aaaaaagtga tatttatctt ggcttaggtg
      901 aaacttacaa gatagatttt attttagttg caaaagctac tgatttagga gaagtaactg
      961 ttaaaacaac ttctaaaaaa acaactgatt ttactagtaa aggaggtgcc gaaactttta
     1021 ttactaaaga aaaaatggat aacttaccaa cagttggtag aaacttacaa gactatttac
     1081 gttttgtacc acaagcaaaa gtggcaagct ctgatggtaa cttagctgga gtttcatttg
     1141 gtgggcaaaa caatcgttat aactcttttt atgtggatgg tgctgctaat aacgaccaat
     1201 ttggtttagc ttcatctggt actaatggtg gccaaacagg ttcttcacca atttcaattg
     1261 atgcaataga tcaaattcaa attatccttt ctccttacga tgcctctatt ggtaatttta
     1321 ctggtgctgg tattaatgct acaactaaaa gtggtactaa taaattgcaa gcttctgttt
     1381 atactttctt ccgcaaccaa gatttaacag gtaaaacacc taatggcgat aaaaaacttg
     1441 caactaaatt agccaagttt tctagcaaaa cttatggttt tagagttgga ggcccaattg
     1501 taaaaaataa agctttcttc tttttaaatg ttgatttagt tagagacgac agaccgcaac
     1561 cttggactgg tacttatgca ggtactacac ctttaaatag ccctaatttt actgctctaa
     1621 tgactaaatt aagtggttat gggtacgacc ccggtggata tgaaaataat gttgaagaag
     1681 taaactctaa tagattagct gcaaagattg attggaattt gaataccact cataaactta
     1741 ctttatctta tagatatagc aaatctgata gatttaatgt gcctacaagc tctaacactt
     1801 ctattaactt ttacaatggt ggctatttaa tgccaaacgt aacacattct ggttctgctg
     1861 agttacgtag tattttcaaa aaaggtgcta ccaataaatt attattaact tttactgatg
     1921 ttacagatga tagaggtcct ttaggaaaag catttccaag agtaactata aatgatggtt
     1981 caggtagatt tgtgtttggt actgaaaact tttctacagc taacttatta actcagaaga
     2041 attttgcttt cttagattat tttaaattta ttaaaggaaa ccatagtttt actattggta
     2101 cagataatga atatacaaaa gcaagaaacg tatttatcag agacaattat ggtaattaca
     2161 cttttgctaa cttgaatgat ttcttaaata atgcttatcc ttctgtttac tctcgttcat
     2221 tttctttgtt agataataaa acaggagatg caactgcatc ggctgctaaa tttagctttg
     2281 cccgtttagc cggatttgta aacgatgaaa ttagtatggg agataacttt actttaaatg
     2341 ttggcttacg tttagactat acaacatttt tatattctcc tgctaaagac ccatatttta
     2401 atgatattgc cttaccaaaa attgctgcac attacgattt acaaggtgca agatctggta
     2461 aaatatccaa ccctaaagct agcctttctc ctcgtatagg atttacttat aaaataccag
     2521 aagagaatgt agtatttaga ggtggtgctg gattatttgc aggaagaatt cctctagtat
     2581 ggcctggtgg tgtgtacaac aatacaggtt tatctgtagg tggtgtaagc ttaaactcaa
     2641 ttacagctac cactgcagct aatttacact ttattgcaga cccttataat cagccaactg
     2701 ctcaaagttt aggtattaat attagcaatg ctaaaggtca ggtagattta attgcaaaag
     2761 actttagatt accaaaagta tttagagcat ctttagcatt tgataaaaga tttgataatg
     2821 gatggtcatt aacagccgaa gttattttaa ctaaaaatat caacgaaatt tactatcaaa
     2881 acttaaatat tttacctcca acattaaaag caacaggacc agataatcgt acagtatatg
     2941 ctgcaggaaa tgcttctgct ccttatattc ctattaataa caataatacc aacccttata
     3001 ctggtatata tttaataagc aacaaaaaag ataaaaaagg gtttgctcat aactttactg
     3061 taactgcaaa taaaaacttc actaatggtt tatcatttaa tgcaagttat gcttatggat
     3121 attctgaagt aacttatgaa ggtacaagta gccaaaacaa tagccaatat aatacacttg
     3181 caactgtaaa tggtagaaat tttgccgaca gacatcgtag taatttcgat ttaggacata
     3241 gaattactgc atttttatct aagaagttta cttatgcaag aaaatcaatg tcaactactg
     3301 ttagcttatt ctatactggg caaagtggaa atcctttaac ttatgtttat aatggctcaa
     3361 tggttagaga tgttagtact gcaaacagcc aagatttaat ttacatacca actgctgccg
     3421 atattactaa tgcagtattt gttacttctg gtggcttaac tcctgctcaa caaaaagctg
     3481 ctttagaaac atttatttct ggtaataaat atttaagttc tagaagaggg caatatgctg
     3541 aaaacaatgg tgatagatta ccatttacca atgtattaga cttgagtatt aaacaagact
     3601 ttaatttaaa aattggtaaa aacaaatacc aaattcaagt tagctacgat atctttaact
     3661 tcagcaatat gttgaataga gattggggtc gccaatactt tgtaaacttt aatacctatt
     3721 cattaatgtc gtttagaggt tatcaaccta acactacaat accaacctat agttttgtac
     3781 cacctgcttc aggttcagta catactgtaa gtacaagtac tgcaccttcg ttcagtgctc
     3841 gttgggtaag ccaattaggt gttcgtttaa gtttatttta gtaggtacta aatattccta
     3901 taaaagaggt tgtcttttag acaacctctt ttgtttttag tgcaactatt tagtttattt
     3961 ttaaaggctt tggttacttt ttttaaaaac aaaattctta taagcaatat gcataatcaa
     4021 atcggcaaaa agctttaaca ctacaaatac aatagatgga gatatagaaa gaaatttagg
     4081 tagcagtatc accaaatgca taggaacaat acgggcataa ggcaaaacaa acatgttacc
     4141 aatgtttaca ggttgtgttc tgtttctagc cttatcttga agatactgaa taatgcttcc
     4201 cgctaagatt gcccaaaaag aaagttttat aaatgtaaat tgagaaggtg taatatcttt
     4261 tatataagaa attaaaaaga acatgtacgc aaaatgaaac aaaccataat gaaaagtaaa
     4321 aaataaagct gcacaaccac ttctattacc tgctttatcg ttaattgtaa aagagttttc
     4381 aactctattg gtaaaagtaa gcataccaat tacattaaaa a
//
LOCUS       DAOQPK010000185         4353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_13082_length_4353_cov_4.491624, whole genome shotgun sequence.
ACCESSION   DAOQPK010000185 DAOQPK010000000
VERSION     DAOQPK010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13082_length_4353_cov_4.491624"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..893
                     /locus_tag="PLU36_08520"
     CDS             174..893
                     /locus_tag="PLU36_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HMZ46832.1"
                     /translation="MNVQLFVPCFIDQLYPNVAFNMLKVLEKAGCIVRYNSNQTCCGQ
                     PAFNAGFWGEARDVCQKFLEDFSHEGYIVAPSASCIGFLKNYYSKIFEGTQYLEKVKN
                     LSDRAFEFSDFMINVLNIDDVGAIFNEKVTYHDSCAALRECKIKTEPRRLLSKVKGLE
                     LVEMNDVETCCGFGGTFAVKFEAISIAMADQKITNAEATKADYIVSTDMSCLMHIDGC
                     IQHKHKNLKVIHLADVLASGW"
     gene            920..1324
                     /locus_tag="PLU36_08525"
     CDS             920..1324
                     /locus_tag="PLU36_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HMZ46833.1"
                     /translation="MNYWLVKSEPFKYSWQQFEKEGQTFWDGVRNYAARNNLKAMKKG
                     DLVFWYHSNEGLEIVGIAQVIKEAYQDPTTEDEAWVVVDLKPYKKLKKPVSLAQIKAE
                     KTLANMDLVRLSRLSVGTVRKQEWEKIIEMSK"
     gene            1418..2347
                     /locus_tag="PLU36_08530"
     CDS             1418..2347
                     /locus_tag="PLU36_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46834.1"
                     /translation="MKKGSIVLLLMSFVFSLHAQNIEGSWYGIGKAETEYSEGNTYLT
                     ELSLTQNGRNITGQLNFYFRDSLFSNTVTGTYDHATRALVFKPTKIIYYKSNNTLTGV
                     DCEVEAYFSLRFSRVESVLTGAFYATNNFKYTCPTINLKLKKNDVVVQEEEVISTIYE
                     EEPTTPKELITTKVMEVSDPSLPLKGSKEIHQNFIKREKIFAKEIEVENNELKLEFYD
                     NGAIDNDSISVFLNDKLVLAKTKLEHKPIQLSIQYNDSLPYNELSMYAESLGLIPPNT
                     SALIIYDGKKRYEVLLTSDFNKNGTIKLTRKKY"
     gene            complement(2365..3855)
                     /gene="pyk"
                     /locus_tag="PLU36_08535"
     CDS             complement(2365..3855)
                     /gene="pyk"
                     /locus_tag="PLU36_08535"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HMZ46835.1"
                     /translation="MTKDLAKYTHAQMDKEAGIKHVSHKTKIVATVGPSCDTYEKLLE
                     LVQTGVNVFRLNFSHGTNDQKATIIQHIRQINGTQPFNISILADLQGPKLRVGEIENN
                     ALNLEPGDVLTLTNEKCVGNKEKVYVSYPNLADDVRIGNIILIDDGKIEVKVIDITKD
                     KNVKVSVTVGGILSSKKGINLPDTKISLPALTEKDLIDLDFIIEQKCDWVALSFVRSV
                     KDIVILRSKLDEKKSKTKIIAKIEKPEALVSLRDIIVESDAIMIARGDLGVELPVEQV
                     PLIQREIIRKCLHRAKPVIVATQMMESMIERVKPNRSEITDVANAVLEGADAVMLSGE
                     TATGNHPALVVETMRKIILQVENTEYRYNRENDLMPQAHSPSFLSDAICYNACKIARD
                     VNADALIGMTQSGYTGFMLSSFRPKSPLYVFSKEKSLINQLSLSWGVRAFYYDEEESV
                     DGIITDEINILKERGFLQSGNIVINTGSTPVTLHLPTNMIKVTQVE"
     gene            complement(3888..>4353)
                     /locus_tag="PLU36_08540"
     CDS             complement(3888..>4353)
                     /locus_tag="PLU36_08540"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HMZ46836.1"
                     /translation="MGRDAGYIALHSGIATGAENILIPETETDMEELICSLTEKEKRK
                     KLVNLVVVAEGGDLGGANKVAKIISERIPTADIRVCILGHIQRGGSPTCQDRLIASHM
                     GYYAVESLMLGRKNVMVGVINNKIFYTPLDKAVKEKQKIGQEWMKIVKILAS"
BASE COUNT         1465 a          641 c          696 g         1551 t
ORIGIN      
        1 tgtgctgtaa aaatagtgtt tacggctttt atttacaaca ttcagttata catattaatt
       61 ttaaaagatt ttaaaactaa tttctgaggt acagagttaa tgcttaaaaa agatactttt
      121 gtaaactgcg gctcaatttt tttaaaaaaa agtagattag taaataagtt tatatgaatg
      181 tacaattatt tgtgccttgc tttattgatc aattataccc aaacgttgct tttaatatgc
      241 taaaagtgtt ggagaaagca ggctgcattg ttaggtataa cagtaatcaa acctgttgtg
      301 ggcaaccagc ttttaatgca ggtttttggg gcgaagcaag agatgtttgc caaaaatttt
      361 tagaagactt ttctcacgaa ggttatattg tagcaccaag tgcaagttgt attggttttt
      421 taaaaaatta ttattctaaa atttttgaag gtacacagta cttagaaaaa gtaaaaaact
      481 tatctgatag agcttttgag tttagtgatt ttatgattaa tgttttaaat atagatgatg
      541 taggtgctat atttaatgag aaagtaactt atcatgatag ttgtgctgct ttaagggaat
      601 gtaaaattaa aacagaacca agaaggttgc taagtaaagt aaaaggatta gaattggttg
      661 aaatgaatga tgtagaaact tgctgtggct ttggtggcac atttgctgta aaatttgaag
      721 caattagtat tgctatggca gatcagaaaa taaccaatgc agaagcaaca aaggcagatt
      781 atattgttag tacagatatg agttgtttaa tgcatattga tggctgtatt caacataaac
      841 ataaaaactt aaaagtaatt caccttgcag atgttttagc ttcggggtgg taattttttt
      901 ataatctaat ttttttaaga tgaattattg gttagttaaa tcggagccat ttaaatacag
      961 ttggcaacag tttgaaaaag agggtcaaac attttgggat ggtgtaagaa attatgctgc
     1021 aagaaataat ttaaaagcca tgaaaaaagg cgatttagtt ttttggtatc atagcaacga
     1081 ggggcttgaa attgttggta ttgcacaggt tataaaagaa gcttatcaag acccaaccac
     1141 agaggacgaa gcctgggttg tagttgattt aaaaccgtat aaaaaattaa aaaaacctgt
     1201 tagccttgct caaattaagg ctgaaaaaac tttagcaaat atggatttgg tgaggcttag
     1261 cagattatct gttggtactg taagaaaaca agagtgggaa aagattattg aaatgagcaa
     1321 ataaggtgtt ttttaaagca aataattcac attaaaaggc aatctgttag caacttttaa
     1381 ctttgtttat acaaactgat tatgttcttt gcagaatatg aaaaaaggga gtattgtttt
     1441 gttgttaatg agttttgtat ttagcctaca tgcacaaaat attgaaggta gttggtatgg
     1501 cattggaaag gctgaaactg aatacagcga aggaaatact tatttaactg aattgagttt
     1561 aacacaaaat gggagaaata taacagggca attgaatttt tattttagag atagcctttt
     1621 ttctaatact gttacaggta cttacgacca tgctacaaga gcactggttt ttaaacctac
     1681 aaaaattatt tactataaat ctaataatac tttaacaggg gttgattgtg aagtagaggc
     1741 atatttttct ttacgttttt ctagagtaga atcggtatta acaggggctt tttatgctac
     1801 taataatttt aaatatactt gccctactat taatttaaaa ttaaaaaaga atgatgttgt
     1861 agttcaagaa gaagaagtta tctcaactat atatgaagag gaaccaacta caccaaagga
     1921 attaattaca accaaagtta tggaggtttc agacccttca ttacctttaa aaggaagtaa
     1981 agaaatacat caaaatttta taaaaagaga aaaaatattt gcaaaagaaa ttgaagttga
     2041 aaataatgaa ttgaaattag aattttatga taatggtgca atagataacg attctatttc
     2101 ggtattttta aatgataaat tagtattagc taaaactaaa ttggagcata aaccaataca
     2161 actttctatt caatataacg atagtttacc ctacaacgaa ttaagcatgt atgccgaaag
     2221 tttaggctta attccgccca atacatctgc cttaattatt tatgatggga aaaaaaggta
     2281 tgaagtttta ttaaccagcg atttcaataa aaatggtaca ataaaactta ctagaaaaaa
     2341 gtactaaaag atactttaca ataattattc tacctgtgta actttaatca tattagtagg
     2401 taaatgtagg gttacaggtg tactaccagt attaataaca atattaccag attgtaagaa
     2461 tcctctttct tttaaaatat ttatttcatc tgttataata ccatctacac tttcttcttc
     2521 atcataataa aatgctctaa caccccagct taaacttaat tggttaatta aacttttttc
     2581 tttagaaaaa acatataaag gagactttgg cctaaagctg ctaagcataa agcctgtata
     2641 gccactttgt gtcataccaa ttaatgcatc tgcattaaca tctcttgcta ttttacaggc
     2701 attgtaacaa attgcatcgc ttaaaaaaga tggggaatgt gcttgaggca ttaaatcatt
     2761 ttctctgttg tatctgtatt cggtattttc aacctgtaaa attattttac gcatagtttc
     2821 aacaacaagt gcaggatgat ttcctgtagc agtttctcca cttagcatta cagcatctgc
     2881 tccttctagt acagcattgg caacatctgt aatttcactt ctgtttggtt ttacacgttc
     2941 tatcatgctt tccatcattt gtgtagctac aattacaggc tttgctctat gtaagcattt
     3001 tctaataatt tctctttgaa ttaatggaac ttgttctact ggtagctcaa cacctaaatc
     3061 tccacgagca atcataattg catcgctttc tacaattatg tctctaaggc ttactaatgc
     3121 ttctggcttt tctatttttg caataatttt tgttttactt tttttctcat caagcttact
     3181 tcttagtatt acaatatctt ttacacttct tacaaaacta agtgctaccc aatcgcattt
     3241 ttgttcaata ataaaatcta aatcaattaa atctttttcg gttaatgctg gtaaagaaat
     3301 ttttgtgtca ggtaaattaa tacctttttt agaagataat attccaccaa cagtaacaga
     3361 tacttttaca tttttatctt tagtaatatc tattactttt acttcaattt ttccatcatc
     3421 tattaaaatt atatttccta ttctaacatc atctgcaagg ttaggataac taacataaac
     3481 tttttctttg ttgccaacac acttttcatt ggtaagtgtt aatacgtctc caggttcaag
     3541 gtttaaagca ttgttttcaa tttctcccac tcttaattta gggccttgta aatctgctaa
     3601 aatagatata ttaaaaggtt gtgtgccatt tatttggcgt atatgttgaa taatggttgc
     3661 tttttgatcg ttggtgccat gagaaaaatt taatctaaaa acattaacac ctgtttgtac
     3721 taattctaat aatttttcat aagtatcgca agagggacca acagttgcaa caatttttgt
     3781 tttgtgagat acgtgtttaa taccagcttc cttatccatt tgagcatgtg tgtatttagc
     3841 taagtctttt gtcatattgt atatactaat tgtatttgaa taatttttta tgaggcaaga
     3901 atttttacaa tcttcatcca ttcttgccca attttttgtt tttcttttac agctttatct
     3961 aatggggtat agaaaatttt attattaata acgccaacca ttacattctt tcttcctagc
     4021 attaaacttt ccacagcata ataacccatg tggcttgcaa ttaacctgtc ttggcaagta
     4081 ggagagccgc cacgttgtat atgccctaaa atacatactc taatatcggc agtaggtatt
     4141 ctttcagaaa taattttagc tactttgttt gcaccgccca aatcgcctcc ttcagcaaca
     4201 accacaaggt ttactaattt ttttcttttt tctttttcgg ttaaagagca aattagctcc
     4261 tccatatctg tttcagtttc cggaattaaa atattttctg cacctgtagc aataccacta
     4321 tgtaaagcaa tatagccagc atctctaccc att
//
LOCUS       DAOQPK010000186         4348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_13118_length_4348_cov_4.804100, whole genome shotgun sequence.
ACCESSION   DAOQPK010000186 DAOQPK010000000
VERSION     DAOQPK010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13118_length_4348_cov_4.804100"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..1042)
                     /locus_tag="PLU36_08545"
     CDS             complement(167..1042)
                     /locus_tag="PLU36_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46837.1"
                     /translation="MKHTIVKQTLFSLISILFFVATTAFTVFAIKYDVNSVREKILKT
                     VFVGEYYSNGSTPEEIAKYYPDTTITFKKPDGVDSKAKYILQTLNYLPVYEFPMLLAD
                     IKDSELNDPNFDYEKIDDRIKEIYGTPLKGKTLNQANGRTFYFYNGAGIVAIFNKLYA
                     KPTNKLENVTYQKIYNLCAKNYVRDFLKLMTYLNTTKKTEWNSECARLLKQIKTDKDF
                     SHYDEYAKTANILFPSEKIRYSFESYTYDVSSTFSLGTIIRRQLDGSLKSITDCLKKI
                     VKDYDPEALKLIKGY"
     gene            1365..2942
                     /locus_tag="PLU36_08550"
     CDS             1365..2942
                     /locus_tag="PLU36_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HMZ46838.1"
                     /translation="MATTDQFIQSINNGYTFKGEHVKIGVGMLDGKAVSDASVNIPFK
                     TLNRHGLVAGATGTGKTKTLQMLTECFSNNSIPVLVMDIKGDFSGIAQAGSLNDKIAE
                     RYALLKETYTPAAFPVEFLTLSDEKGARLRATVSEFGPILLSKILGLNDIQSGLVAVL
                     FKYCDDNKLPLLDLKDFIKVLQYAGDEGKDELEKSYGKISTTSTGTILRKVIELQQQG
                     ADVFFGERSFEVDDLMRINNEGKGMVNVLRLMDMQNRPKLFSTFMLQLLAELYANCPE
                     EGDLDKPKLIMFIDEAHLIFQEASSALMQQIETVIKLIRSKGIGIFFCTQNPQDIPAS
                     VLAQLGLKVQHALRAFTAADRKVIKTAAENFPETTFYQTNELLTQLGIGEAFITLLNE
                     KGIPTPLVHTLLCSPKSRMDVISDAEMDAVIAESRLVKKYNEVIDSQSAYEILTEKLN
                     IAEEKTKEEQQQKEQAKEEEKQEKEEAKKEKDEPGFFDNPTVKQIGRTAASVITRSLL
                     GALGISSYTRKKRKSLW"
     gene            complement(2987..3787)
                     /locus_tag="PLU36_08555"
     CDS             complement(2987..3787)
                     /locus_tag="PLU36_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HMZ46839.1"
                     /translation="MNSLTFSIIQTNLFWEDKAKNLEMLEQKIASIKSKTEIVVLPEM
                     FSTGFSMQPEKYAENMDGICVNWMKTIATKYKIILTGSLMIKEKETFYNRLVWMLPNG
                     KYGFYNKRHCFAYAKENEHYTPGNNRFIASVNGWKINLQICYDLRFPVWARQASNQLT
                     PEYDILLYVANWPQRRSHAWKTLLTARAIENQCYVIGVNRIGTDGNDIYHSGDSMVIN
                     ALGEVLYQKADEEDVFTITLQKNDLEEIRTKFPFLADADDFNLLQVPN"
     gene            complement(3835..>4348)
                     /locus_tag="PLU36_08560"
     CDS             complement(3835..>4348)
                     /locus_tag="PLU36_08560"
                     /inference="COORDINATES: protein motif:HMM:NF021497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2219 family protein"
                     /protein_id="HMZ46840.1"
                     /translation="YWQTQIPNQFGINVGFKYATSITNTNNQFSKFKIVPIAEANVGT
                     FFINAKTGAYFCLGKFKKNSNSALFNAVIQKKDNPINNSNEFFLYLYPQIIYQAYNAT
                     VQGNIFRKETNPNVFETSVNPFMFQQTLGVAYTKNRWTTKLELVYQTKEAKTQVKDNG
                     YAGLHFAYRF"
BASE COUNT         1494 a          636 c          691 g         1527 t
ORIGIN      
        1 caaagccatt cccatatttg ttttgctctg aatttttttt cacctaattg caaaaaataa
       61 ttttcaatat cgttatagct taaatgtctg atattttgca tagcggcaaa gatagcatga
      121 aattaaaagc tatttagaat agattgttct tgcataagcc aaccaattaa taccctttaa
      181 tcaattttaa tgcctctggg tcataatctt ttactatttt ctttaaacaa tctgtaattg
      241 actttaaact tccatctaat tgtcttcgta taatggttcc taaggagaag gttgaactaa
      301 catcataagt ataagattca aagctatacc ttattttttc tgatgggaaa agtatatttg
      361 cagttttagc gtactcatcg taatgactaa aatctttatc tgtttttatc tgttttaata
      421 gtcttgcaca ttccgagttc cattcagttt ttttagttgt atttaaatag gtcattaatt
      481 ttagaaaatc acgtacataa tttttagcac ataaattgta aattttttga taggtaacat
      541 tttctaattt gttagttggt tttgcataaa gtttattaaa aatagcaaca ataccagcac
      601 cattataaaa ataaaaagtt cttccgtttg cctggtttaa agttttgcct tttaaaggtg
      661 tgccataaat ttctttaatt ctgtcatcta tcttttcata atcaaaattt ggatcattta
      721 attctgaatc ttttatatcg gccaataaca ttggaaattc atacacaggt aaatagttca
      781 aagtttgtaa gatatatttt gcttttgaat ctacaccatc gggtttttta aatgttatgg
      841 ttgtatcggg ataatatttt gcaatttctt ctggtgttga gccgtttgaa taatactctc
      901 ctacaaaaac tgtttttagt atcttttctc taacgctatt tacatcgtat ttaattgcaa
      961 atacagtaaa tgcagttgtt gctacaaaaa ataatatact aattaatgaa aataaagttt
     1021 gctttacaat agtatgtttc atataaaaat tttgatttaa agatatacta attcaatcat
     1081 taaattatac ctccttttgg gtattttttg gcaattgcaa cctttgtatt ctattcgtcg
     1141 tctattaaaa tgtaaattct ttgttatgaa aaagtttctt acaactacca acgatacttc
     1201 agaaattgcc ttagtaatta ttagtgttat tttaatgact atttcaacac tttttgtaca
     1261 ctagattgat tttatctttc tttttccatt aattttttag aagtattttt tattgtatga
     1321 atacagaata aaaaatacca ttatcttgcc tgtaaatttt tacaatggca actaccgatc
     1381 aatttataca aagcattaac aatggctaca cttttaaagg agagcatgtt aaaattggtg
     1441 taggaatgtt agatggaaaa gctgtaagcg atgcttcagt gaatattcct tttaaaacct
     1501 taaacagaca tggcttggta gctggtgcaa caggcacagg taaaaccaaa accttgcaaa
     1561 tgcttacgga gtgttttagt aacaatagta ttcctgtttt ggtaatggat attaaaggag
     1621 attttagtgg tattgcacaa gcaggtagtt taaatgataa aattgcagag cgttatgctt
     1681 tattaaaaga aacctataca cctgctgctt ttcctgtaga gtttttaaca ttaagtgatg
     1741 aaaagggtgc taggttaagg gctacagtta gtgaatttgg acctatttta cttagtaaaa
     1801 ttttaggcct taatgatatt caaagtggtt tggttgctgt attatttaag tattgcgatg
     1861 ataataagct acccttatta gatttgaaag actttattaa agttctacaa tatgcaggag
     1921 atgaaggaaa agatgaatta gagaaatcct atggaaaaat ttcaactacc agtacaggta
     1981 caatacttag aaaagttata gaattacaac aacagggtgc agatgtgttt tttggagaaa
     2041 ggagttttga agtagatgat ttaatgcgaa ttaataatga aggaaaagga atggtaaacg
     2101 ttttaaggct aatggatatg caaaacagac caaaactttt ctctacattt atgctgcaac
     2161 tgttggctga attatatgca aactgcccag aggaaggaga tttggataag cctaaactaa
     2221 ttatgtttat tgatgaggca catttaattt ttcaggaggc aagtagtgca ttaatgcaac
     2281 aaatagaaac ggttattaaa ttaattagaa gtaagggtat tggcattttc ttttgtacgc
     2341 aaaacccaca agatattcca gcaagtgttt tagcacaatt aggattaaaa gtgcagcatg
     2401 cactaagagc atttactgca gcagatagaa aagtaataaa aactgcggca gagaattttc
     2461 ctgaaacaac tttttaccaa actaatgagt tacttacaca attaggaata ggtgaagctt
     2521 ttattacttt actcaacgaa aaaggaatac ctacaccact ggtgcacacc ttactctgtt
     2581 ctcctaaaag tagaatggat gttatttctg atgcagaaat ggatgcagta attgcagaaa
     2641 gtagattagt gaaaaaatac aatgaagtga ttgatagcca aagtgcttat gaaatactta
     2701 ctgaaaaact aaatattgcc gaagaaaaaa caaaggaaga acaacaacaa aaagagcaag
     2761 caaaagaaga agaaaaacaa gaaaaagaag aagcgaaaaa agaaaaagac gaaccaggct
     2821 tttttgataa tccaacagta aaacaaattg gcagaactgc agcaagcgta ataacaagaa
     2881 gcttattagg ggcattgggc attagcagtt atacacgcaa aaaaagaaaa agtttatggt
     2941 aaaattagtt gtgtgaggct gtgttatagt atttggttta aaaaatttaa tttggcactt
     3001 gcagtaaatt aaagtcatcg gcatcggcta aaaaaggaaa ctttgttctt atctcctcca
     3061 agtcattttt ttggagagtt atcgtaaata catcttcttc atcggctttt tggtataaaa
     3121 cttctcctaa agcatttata accatactat caccactatg ataaatatcg ttaccatcag
     3181 tacctattct gtttacacca ataacgtaac actggttttc aattgctctt gcagtaagta
     3241 atgttttcca tgcatggctt cttctttgcg gccaattggc tacatacaat aaaatatcat
     3301 actcaggagt tagttggtta ctggcttgcc ttgcccaaac aggaaatctt aaatcgtaac
     3361 aaatttgtaa attaattttc caaccgttaa cacttgctat aaatctatta ttaccaggtg
     3421 tgtaatgttc attctctttg gcataagcaa agcaatggcg tttattgtaa aaaccatact
     3481 ttccattggg tagcatccaa accaaacgat tataaaatgt ctccttttct ttaatcatca
     3541 aacttcctgt aagaataatt ttatattttg ttgcaatggt tttcatccag tttacacaaa
     3601 taccatccat gttttctgca tacttttcag gctgcatgct aaagcctgta ctaaacattt
     3661 ctggcaaaac aacaatttca gttttggatt taattgaagc aattttttgc tcaagcattt
     3721 ctaggttttt tgctttatcc tcccaaaaaa gattggtttg tattattgaa aaagtaaggc
     3781 tattcataat taattaatta atgattgaac aagtaaagaa ggagagcaaa tttattaaaa
     3841 tctgtatgca aaatgcaaac ctgcatagcc attatcttta acttgtgttt tagcctcctt
     3901 agtttgatat accaattcta acttggtggt ccatctattt tttgtataag caacacctaa
     3961 agtttgttga aacataaaag gattaactga agtttcgaac acattaggat tggtttcttt
     4021 tctaaaaata tttccttgaa cagttgcatt atatgcctga taaataattt gaggatataa
     4081 ataaagaaaa aattcgttac tattatttat tgggttatcc tttttttgaa taactgcatt
     4141 aaacaaagca ctattgctat tttttttaaa tttacctaaa caaaagtaag ctcctgtttt
     4201 tgcatttata aaaaaggttc caacattagc ttctgctata ggtacaattt taaatttgct
     4261 aaattgatta ttggtatttg ttatacttgt tgcatattta aaaccaacat ttataccaaa
     4321 ttgattggga atttgagttt gccaatat
//
LOCUS       DAOQPK010000187         4335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_13173_length_4335_cov_3.860047, whole genome shotgun sequence.
ACCESSION   DAOQPK010000187 DAOQPK010000000
VERSION     DAOQPK010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13173_length_4335_cov_3.860047"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1615
                     /gene="bshC"
                     /locus_tag="PLU36_08565"
     CDS             <1..1615
                     /gene="bshC"
                     /locus_tag="PLU36_08565"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HMZ46841.1"
                     /translation="KINCSHLTYKQTGFFSKIVLSYLNSDKELQPFFEHNTTNEGLKN
                     AIASRKNFKTNRKLLVEVLEQQYQNIPLTALQEKNLKALLHENTFTVTTAHQPNIFTG
                     PLYFIYKILHVIKLADDWNKHDNTNYFVPIYFMGSEDADFEELSFINLEGEKLSWKTN
                     QTGAIGRMLVDENLIQLIDKIAGQIGIYNFGNELITNIKLCYTLNKNIEQATIEFVNI
                     LFKEFGLLILIPDNSLLKNEFKPIIEKELAENFSNKYLEKTVNELNKNFKIQTLGRQI
                     NLFYLQDNQRYRIIKEEGKFGFKNTSNEFQQLDIQYELKNSIKAFSPNVILRGVFQEM
                     ILPNIAFVGGGGELAYWLELKNIFNAVNVPYPVLILRNSFLIVDEKNTRKINQLLLQD
                     DALFLNENDLINSQIFPKIEQEFSLKKNKAIEEINDGFANLISSLQNNYPTLAIHTKA
                     LHTNSVKRIQALEKKILKAEKRKNEEVVQKLKNVKHSLFPKNNLQERVENFAILYAKY
                     GAEFLQVLFQHSLTTEQLFTILKLDSKVE"
     gene            1672..2148
                     /locus_tag="PLU36_08570"
     CDS             1672..2148
                     /locus_tag="PLU36_08570"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMZ46842.1"
                     /translation="MSFQKITPCLWVEKDAKIVAEYYLSIFKNGILKDFRLFENMPGA
                     DGTGNDGSFETAVVELEGVEFSILAAGPMFKFNEAISLVINCKNQDEVDYYWNALTSN
                     GGEEGVCGWCKDKYGLSWQVVPIEYFALINSNDSAVREKALLNTFKQKKLILSELK"
     gene            complement(2159..3148)
                     /locus_tag="PLU36_08575"
     CDS             complement(2159..3148)
                     /locus_tag="PLU36_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HMZ46843.1"
                     /translation="MKFEELFRKIKQLKVGIVGDVMLDTYMWGNVERISPEAPVPIVS
                     LKNKENRIGGAGNVALNCIALQANTHIFSVIGNDEDGNKLIQLLNENNIDTGGIIKSS
                     NRITTNKLRIIARNQQMMRLDAEITNDINAIEECELLTKLELFIATQNPDVIIFEDYN
                     KGVLTEKLIAKAIQLCKENDIITSVDPKHKNFFNFKGVTIFKPNFKETIEALGIVDNI
                     ISLEQLNKIHHQLKEKLQHHISFITLSEKGVYYNSGNEANLMEAHIRKIADVSGAGDT
                     VIAVASLVYAASKNISLSTQIANIAGGLVCEQVGTVAINKMALMQEIYKLLVE"
     gene            complement(3152..>4335)
                     /locus_tag="PLU36_08580"
     CDS             complement(3152..>4335)
                     /locus_tag="PLU36_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HMZ46844.1"
                     /translation="EAYNPLIGFLYGWSFFAVIQTGTIAAVGVAFAKFLAYLVPELGN
                     SYFLFQIGFLKIYSGQLVAIVIIILLTYVNSRGVKEGKWIQTLFTSAKLLALIGLIVF
                     GFVLVKESYWDENWKTGFNSLQNKGIKDAHGLLQPADWIGISGSALMGAIAASMVGSI
                     FSSDAWNNVTFIAGEMKNPQRNIGLSLFLGTLIVTIIYVSANLMYLNVLPLNELAFPT
                     DDRVAVAAANKIFPSFGTTFIAVLIMVSTFGCNNGLILAGARVYYTMAQDGLFFKKAA
                     TLNKNNVPQWALWAQCIVASVLCLSGKYGDLLDMISFVVVIFYALTIWGIFILRKKQP
                     NVIRPYKAFGYPFLPLLYIIMGLAFCVLLVIYKPMYTWPGLIIVLIGIPIYYIAVGKK
                     K"
BASE COUNT         1596 a          623 c          649 g         1467 t
ORIGIN      
        1 gaagattaat tgtagccatt taacttataa acaaacaggt tttttttcta aaatagtttt
       61 aagttatttg aatagtgata aggaattgca accttttttt gaacataata ctactaacga
      121 aggtttaaaa aatgctattg cctctagaaa aaattttaaa accaatagaa aattattagt
      181 agaggtttta gaacaacaat accaaaacat acctcttact gccttacaag aaaaaaattt
      241 aaaagcctta ttacacgaaa atacttttac tgtaacaact gcccatcaac caaatatttt
      301 tactgggcca ttatatttta tttataaaat tttgcatgtt attaagctgg cagatgattg
      361 gaataaacat gataatacca attattttgt gcctatttat tttatgggta gcgaagatgc
      421 agactttgaa gagttgagtt ttattaattt agaaggagag aagttaagct ggaaaaccaa
      481 tcaaacaggt gctattggta gaatgttggt ggatgaaaac ttaattcaat taattgataa
      541 aattgctggg caaattggca tatataattt tggtaatgaa ttgattacta atataaaact
      601 atgctacacc ttaaataaaa atatagaaca ggcaacaatt gaatttgtaa atattttatt
      661 taaagagttt ggtttactga tacttatacc agataactca cttttaaaga acgaatttaa
      721 gccaattatt gaaaaagagt tagctgaaaa tttttctaat aaatatttag aaaaaactgt
      781 taatgagtta aataagaatt ttaaaattca aacattaggt aggcaaatta acctttttta
      841 tttacaagat aatcaacgtt atagaattat taaagaagaa ggtaaatttg gatttaaaaa
      901 tacaagtaat gagtttcaac aacttgatat tcagtatgaa ttaaaaaata gtataaaagc
      961 atttagtccg aatgttatct taagaggcgt ttttcaagaa atgatattac caaatattgc
     1021 ttttgtgggt ggtggtggag aattagcata ttggttagag ttgaaaaata tttttaatgc
     1081 agttaatgtt ccataccctg ttttaatttt aaggaactca tttttaattg ttgatgaaaa
     1141 aaatacaagg aaaattaatc agttgctatt gcaggatgat gccttgtttt taaacgaaaa
     1201 tgatctaatc aacagtcaaa tttttccaaa gattgagcaa gaattttctc taaagaaaaa
     1261 taaagctatt gaagagatta atgatggttt tgccaattta atatctagct tacaaaataa
     1321 ctatccaacc ttggcaattc atacaaaagc attgcataca aattctgtta aaaggataca
     1381 agcattagag aaaaaaattt tgaaggctga aaaaagaaag aatgaagaag tggtgcaaaa
     1441 gctaaaaaat gtaaaacaca gtttatttcc taaaaacaat ttacaagaaa gggtagagaa
     1501 ttttgctatt ctttatgcaa aatatggagc tgaattttta caggttttat ttcaacattc
     1561 tttaacaaca gagcaattgt ttacaatttt aaagttagat agtaaagtgg aataatagtt
     1621 tttaagaatt gtttaggcta agaactaaga aatgtttaat cttttataat aatgagtttt
     1681 caaaaaataa caccttgtct ttgggttgaa aaagatgcaa aaattgttgc tgagtattat
     1741 ctttctattt ttaaaaatgg aatattgaaa gattttagat tgtttgaaaa tatgcctggt
     1801 gcagatggaa ctggaaatga tggaagcttt gaaactgcag ttgtagagtt ggaaggtgta
     1861 gagttttcaa ttcttgctgc tggccctatg tttaagttta acgaagcaat atcacttgta
     1921 ataaactgta aaaatcaaga tgaggttgat tattactgga atgcattaac ctcaaatgga
     1981 ggagaagagg gggtttgtgg ttggtgtaaa gataaatatg gtttatcgtg gcaggtagtt
     2041 cctattgaat actttgcctt aattaatagt aatgatagtg ctgtaagaga gaaagcatta
     2101 ttaaacacgt ttaagcaaaa aaagttaata ttatcagagc ttaaataagg ttcaaaactt
     2161 attcaacaag caatttatag atctcctgca ttaaagccat tttattgatt gcaacagtac
     2221 ctacttgttc gcaaactaag ccacctgcaa tattggcaat ttgtgtagaa agtgaaatat
     2281 ttttagatgc agcatatacc aaagatgcta cagcaataac tgtatcgcca gcaccactta
     2341 catctgcaat ttttcttata tgggcttcca ttaaattggc ttcatttcct gagttgtaat
     2401 aaacaccttt ttccgataaa gtaataaatg atatatggtg ttgtaacttt tcttttaatt
     2461 gatggtgtat tttgttcaat tgttccaagc taattatatt atcaacaatg ccaagtgctt
     2521 ctatggtttc tttaaagttg ggcttaaaaa tagtaacacc tttaaagttg aaaaagtttt
     2581 tatgtttagg gtcaacagaa gtgataatat cattttcttt acataattgt attgcttttg
     2641 ctatcaattt ttcagttaaa actcctttat tgtagtcctc gaaaatgatt acatctggat
     2701 tttgtgttgc aataaataac tctaatttag ttaataactc acattcttca atggcattaa
     2761 tatcattggt aatttcagca tctaaacgca tcatttgctg atttcttgca ataattctaa
     2821 gtttattagt tgtaattctg ttactgcttt taattatccc accagtatca atattatttt
     2881 catttaataa ttgaattagt ttattaccat cttcatcatt tccaattact gaaaaaatat
     2941 gtgtgttagc ttgtaaagca atacagttta aagcaacgtt acctgcacca ccaattctat
     3001 tttctttgtt ttttaaagaa acaattggca caggagcttc tggcgaaatt ctttctacat
     3061 ttccccacat ataagtatct aacattacat cgccaacaat accaactttt aattgtttta
     3121 ttttcctaaa gagttcttca aacttcatga attatttttt tttaccaaca gcaatataat
     3181 aaataggaat tccaattaaa actattatta atccaggcca agtgtacatg ggtttataaa
     3241 taaccaacag tacacaaaat gctaatccca ttataatata tagtaaaggt agaaaaggat
     3301 agccaaaggc tttataaggt cttataacgt tgggttgttt ttttcttaaa ataaaaattc
     3361 cccaaatggt gagggcataa aaaattacca caacaaaaga aatcatatct aacaaatctc
     3421 catacttacc acttaagcat aaaacagaag ctacaataca ttgagcccat aatgcccatt
     3481 gaggaacatt gtttttatta agagtagcag cttttttaaa aaacaatcca tcttgtgcca
     3541 tagtataata aactcttgca ccagccaata ttaaaccatt gttgcaccca aaggttgata
     3601 ccataataag tacagcaata aatgttgtac caaaagaagg gaatatttta tttgctgctg
     3661 ctactgcaac tctatcatct gtaggaaaag ccaattcatt taatggtaat acatttaagt
     3721 acattaaatt ggcactcaca taaattatgg taacaattaa ggttcctaaa aataaactta
     3781 agccaatatt tctttgtgga tttttcattt ctcctgcaat aaaagttaca ttattccagg
     3841 catcgctact aaaaatagat ccaaccatag atgctgcaat ggcacccatt aaagcactgc
     3901 cactaatacc aatccaatct gcaggttgta ataggccatg agcatcttta atacctttat
     3961 tttgtagaga gttaaatccg gttttccaat tctcatccca atagctttct tttactaaaa
     4021 caaatccaaa aacaattaaa ccaattaatg ccaaaagctt tgcacttgta aaaagtgttt
     4081 gtatccattt tccttctttt acacctcttg aattaacgta ggttagtaaa ataataatta
     4141 caatggcaac taattgccca gagtatattt taagaaagcc tatttgaaaa agaaagtaac
     4201 tattgcccaa ttctggaact aaataagcta aaaatttagc aaaagcaacc ccaactgctg
     4261 caatagtacc agtttgaatt actgcaaaaa aactccagcc atacaaaaaa ccaattaatg
     4321 ggttataagc ttctt
//
LOCUS       DAOQPK010000188         4315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_13275_length_4315_cov_4.104225, whole genome shotgun sequence.
ACCESSION   DAOQPK010000188 DAOQPK010000000
VERSION     DAOQPK010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13275_length_4315_cov_4.104225"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="PLU36_08585"
     CDS             <1..340
                     /locus_tag="PLU36_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ46845.1"
                     /translation="PKISAALLVIPCLIVLSAVLAIWGGKIAGVASGIISEDVFDIGL
                     RQNFTPYNVFFALSKAYTFAFIVSSVPAYYGYHVKGGALEIGRTSTTAVVVSCILILL
                     ADYVLAALML"
     gene            432..2003
                     /locus_tag="PLU36_08590"
     CDS             432..2003
                     /locus_tag="PLU36_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HMZ46846.1"
                     /translation="MPIRMVDDDKDNFSNENQNTPGGGGGNRGGGGLMQFLPMIIGLL
                     IRKPALLLVVLAGAAFFYFKGGCNFSSVSNIVQQFSTGGNLNKDTFAKAEVYEGLDES
                     KNNLPEYISLLKFSPNRLNQGQQGSCVAWSSAYAARTIVESTSKGIDPNAAAFSPSFM
                     YNQIGLEGCQGSYIIRAMQNLTQVGAVPFNDFPYTDKDCTNQPNGQLINEAGQNKIIG
                     FTRLTETESAAQIDIYAIKQHLAKDVPVVIGMMVGGSFMQDMMGKEIWHPTADDYNMM
                     GFGGHAMCVIGYDDRKEGGAFQIMNSWGNEWGENGIGWVLYKDFKHFVREAYGLNPMI
                     KQGAAIAKNLEVDLGLVNAETKQYIPLKTVNNNVFETVNPIPKGTGFKIELKNNTECY
                     VYVIGMETDGSSYVLFPYPSSSDATKSMFSPYCGITGYRLFPKGKKLQADDIGNKDYF
                     AIITSKEALDVFQLNNIISQNKGLGFENAINNALQKYTINGVSYNANTNGTIGFKTET
                     NNKNAVACIIAIDKY"
     gene            complement(2188..2475)
                     /locus_tag="PLU36_08595"
     CDS             complement(2188..2475)
                     /locus_tag="PLU36_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46847.1"
                     /translation="MKLNKTQLYLILGVVIVICIPLVAMLFTTEVKWSFFDFVLAAVL
                     LLFVALLCEFLYRKINNIVLKIITCSLVIITCILFWIEIAVGLFNSPFAGS"
     gene            complement(2553..3368)
                     /locus_tag="PLU36_08600"
     CDS             complement(2553..3368)
                     /locus_tag="PLU36_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HMZ46848.1"
                     /translation="MPQEKLTDLHSVYTETLTLTSVYLERDVIIDVYFPNKVDKPENL
                     SLLIFNDGQDLLKMPFDEILDELTSQNIIEPLFCIGIHCGEERKREYGTAYSADFKQR
                     GDKAGLYNKFLFDELLPTIRYAYHIPSFKEKSFAGFSLGGLSALDIVWNHANEFTKVG
                     VFSGSLWWRKKSYEDGYDDENDRLMHLQIRKGTFYPWLKFYFQCGALDEVADRNKNGI
                     IDSIDDALDLIVELKIKGYSDKHIQYVLLEDGKHDTTTWANALPNFLKWGWGK"
     gene            3519..>4315
                     /locus_tag="PLU36_08605"
     CDS             3519..>4315
                     /locus_tag="PLU36_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46849.1"
                     /translation="MRKIGILHGKERSFPEALVAAINSKNIPGIVAEPVRIDKVMQGE
                     PSDYAVIIDRISQDVPFYRAYLKNAALCGTAVINNPFWWSADEKFFNNCLATKIDVPV
                     PKTVILPSRDLPDDTSSESFSNLAYPLDWESIFNYTGFPAYMKPFAGGGWKSVYKLES
                     MEDFFEKHAETKQLVMLLQEEIIFEEYYRCYCIGGKYVRIMPYEPRNPHHLRYVASFT
                     PSAERLKLMEEIVLRINHYLGYDFNTVELAVRNGVPYAIDFCNPAPDA"
BASE COUNT         1510 a          656 c          698 g         1451 t
ORIGIN      
        1 tcctaaaatt tctgcagcat tgctagtaat accttgctta attgttttaa gtgcagtatt
       61 agctatttgg ggtggcaaaa tagctggtgt tgcaagtggt atcatttctg aagatgtttt
      121 tgatataggg ttaaggcaaa actttacacc atacaacgtt ttttttgcac tcagtaaagc
      181 atatactttt gcttttattg ttagcagcgt acctgcttat tatggttatc atgtaaaagg
      241 tggtgcatta gaaattgggc gaacaagtac cactgctgtt gttgtaagtt gtattttaat
      301 tttattagca gattatgttt tagctgcact tatgctctaa aattattaac aaaaatatta
      361 gtttcaagca cttttattat acttgttgta gtattgctat taattttatt ttcattccta
      421 aaatatttat tatgcctata agaatggttg atgacgataa ggataacttt tccaacgaaa
      481 atcaaaatac acctggtggt ggtggaggca acagaggtgg tggtggttta atgcaatttt
      541 tacctatgat tattggtttg cttatacgta aacctgcttt attattagta gtgttagctg
      601 gtgcagcttt cttttatttt aaaggtggtt gcaattttag ttcagttagc aacatagtgc
      661 aacaattttc aacaggtggt aatttaaaca aagatacttt tgctaaagca gaagtttatg
      721 aaggtttaga tgaaagtaaa aataatttgc ccgaatatat ttcattgcta aaattttctc
      781 caaacaggtt aaaccaaggg caacaaggaa gttgtgttgc ttggagtagt gcttatgcgg
      841 ccagaacaat tgtagaatct acaagtaaag gtattgatcc taatgcagct gcttttagcc
      901 catcttttat gtacaaccaa attggcttag aaggatgcca gggtagttac attattcgtg
      961 caatgcaaaa tttaactcaa gtaggagcag ttccattcaa cgattttcct tatacagata
     1021 aagattgtac caaccaacca aatggacagt taataaacga agcaggacaa aataaaataa
     1081 ttggttttac cagattaaca gaaacagaaa gtgctgctca aattgatatc tatgccatca
     1141 aacaacacct tgctaaagat gttcctgttg taataggaat gatggtagga ggaagtttta
     1201 tgcaagatat gatgggtaaa gaaatttggc atcctactgc cgatgactat aatatgatgg
     1261 gctttggagg acatgcaatg tgtgttattg gatacgatga tagaaaagaa ggtggtgcat
     1321 ttcaaataat gaatagttgg ggtaatgagt ggggcgaaaa tggaattggc tgggtattgt
     1381 ataaagattt taaacatttc gttagagaag cgtatggctt aaacccaatg attaaacaag
     1441 gtgccgcaat tgcaaaaaat ttagaagtag atttaggctt ggttaatgca gaaactaaac
     1501 aatacatacc tttaaaaaca gtaaacaaca atgtttttga aactgttaat ccaattccta
     1561 aaggaacagg ttttaaaata gaattaaaaa ataataccga gtgttatgta tatgtaattg
     1621 gaatggaaac agatggaagt agctacgttt tatttcctta tccatcctct tctgatgcaa
     1681 caaaatcaat gttctctcct tattgtggca ttactggtta tcgtttattt ccaaaaggca
     1741 aaaaattaca agccgatgat attggcaata aagattattt tgcaattatt actagtaaag
     1801 aggcattaga tgtttttcag ctaaataata tcatttctca aaacaaaggg ttaggctttg
     1861 aaaacgctat taataatgca ctgcaaaaat atacaataaa tggtgttagt tacaatgcaa
     1921 ataccaacgg aacaataggt tttaaaaccg aaaccaataa caaaaatgct gtagcatgca
     1981 ttattgctat tgacaaatat taattaaaaa atattcatcc ttatttacta atttaatcaa
     2041 tgaagataag tttgagtact aatttaaaat gtaaacaata aagattactt taagtaattt
     2101 ttacaaatgt taagcagtca tttttcaaaa ctctagtaca cactctttat taaattaaag
     2161 aaaatagaaa ttaaaatttc agttttatta acttcctgca aatgggctat taaataagcc
     2221 taccgctatt tctatccaaa ataaaataca tgtaattata accaagctac atgtaataat
     2281 tttcagaact atattattta tttttctgta taaaaactca catagtaaag caacaaaaag
     2341 tagtagtaca gcggcaagta caaaatcaaa aaaactccac tttacttcag tagtaaataa
     2401 catagctact aatggaatac aaataactat tacaacacca agtataagat ataattgagt
     2461 tttgtttagt ttcattaaaa cattttttac aacaataagt tataaagcta atctaaaata
     2521 agtacaagaa ttgttaatcg ttttttaaaa ccctatttgc cccaccccca ttttaaaaaa
     2581 tttggcaaag cattagccca agtggttgta tcatgttttc catcttctaa taaaacatac
     2641 tgtatatgct tatcagaata tccttttatt tttaattcaa caattaagtc taaagcatcg
     2701 tcaatgctat ctataatgcc atttttattt ctatccgcaa cttcatctaa tgccccacat
     2761 tgaaaataaa atttcaacca tgggtaaaaa gtgccttttc ttatttgcaa gtgcattagc
     2821 ctatcgtttt catcatcata accatcctca taactttttt ttcgccacca taaactacca
     2881 ctaaaaacgc ccactttagt aaattcgttt gcatggttcc atacaatatc taaggcactt
     2941 aacccaccta acgaaaaccc agcaaacgat ttttctttaa aagatggtat atgataagca
     3001 tatctgatgg taggcaataa ttcatcaaat aaaaatttat tgtataagcc tgccttatct
     3061 cctctttgtt taaaatctgc actatatgca gtaccatatt ctcttttcct ttcttcgcca
     3121 caatgaattc caatacaaaa caatggttca attatgtttt gagatgttaa ttcgtctaat
     3181 atttcatcaa aaggcatttt cagtaaatct tgcccatcat taaaaattag taaacttaaa
     3241 ttctctggtt tatctacttt attaggaaag taaacatcta ttataacatc acgttctaga
     3301 tatacagatg taagggttag cgtttctgtg taaacagaat gtaagtcagt taatttttcc
     3361 tgaggcatat attttcaaaa ataagggaaa gcattaaaaa taattaaatg attttttcac
     3421 aaaaaatttg tttttcagtc tataagttct atattgaaaa aggaaaaata ataacactta
     3481 tttattcaat cttttttaat aatttaaact atccctttat gagaaaaatt ggtattttac
     3541 acggaaaaga aagaagtttt ccggaagctt tggttgctgc aattaacagt aaaaatatac
     3601 caggaattgt tgctgagcct gtacgtatag acaaagtaat gcaaggcgaa ccatctgatt
     3661 atgctgtaat aatagatcgt attagtcaag atgttccttt ttacagagcc tatttaaaaa
     3721 atgcagcatt atgtggtact gcagtaatta ataacccttt ttggtggagt gcagatgaga
     3781 aattttttaa taattgcttg gcaacaaaaa tagatgttcc tgtacctaaa acagttattc
     3841 taccttctag agatttacca gacgatacct ctagcgaatc ttttagcaat ttagcttacc
     3901 ctttagactg ggaaagtatt tttaattaca ctggctttcc tgcttacatg aaaccatttg
     3961 ctggtggtgg ctggaagagt gtttataagt tagaaagcat ggaagacttt tttgaaaaac
     4021 atgccgaaac gaaacaatta gtaatgttac ttcaagaaga aattattttt gaagaatact
     4081 atcgttgtta ttgcatagga ggaaaatatg tacgaataat gccttacgag ccacgcaatc
     4141 ctcatcattt acgttatgtt gcaagtttta ctccaagtgc agaaaggtta aaactaatgg
     4201 aagaaattgt acttcgtatt aaccattatt taggatacga ttttaataca gttgaattag
     4261 ctgttcgtaa tggcgtacct tatgcaatag atttttgtaa ccctgcacca gatgc
//
LOCUS       DAOQPK010000189         4300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_13348_length_4300_cov_4.942049, whole genome shotgun sequence.
ACCESSION   DAOQPK010000189 DAOQPK010000000
VERSION     DAOQPK010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13348_length_4300_cov_4.942049"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1397)
                     /locus_tag="PLU36_08610"
     CDS             complement(<1..1397)
                     /locus_tag="PLU36_08610"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HMZ46850.1"
                     /translation="MKIFLAQQNYHIGNFELNTQKIIQAIEEAKQKGGDLIVFSEMCV
                     CGYPARDFVEFEDFINKCYHAINKIKEHADSIGVLIGSPARNKIKEGKDLFNASFFLY
                     EKEIKAEVHKTLLPTYDVFDEYRYFEPAYNWNTIDFKGKKIAVTICEDIWNLGDNPLY
                     RICPMDELMKQEPDVMINLSASPFDYTHIEDRKAIIKANVLKYKLPLIYCNTVGSQTE
                     IIFDGASLVFDKNANLIKALPVFKETIDCVELNNDGTFNASVIEMANQISNKELNPST
                     LNEQLNIAQIHDALVLGIKDYFSKMNFTKAILGSSGGIDSAVVLTLACQALGAENVRA
                     VLMPSPYSTEHSVNDAVQLSKNLNNPYDIIRIDNVYESFLSTLIPIFNNLPFSLAEEN
                     LQSRTRGNLLMAIANKFGYILLNTSNKSELATGYGTLYGDMAGGLGVLGDCYKLQVYA
                     LANYINRDKEIIPIN"
     gene            1540..2427
                     /gene="meaB"
                     /locus_tag="PLU36_08615"
     CDS             1540..2427
                     /gene="meaB"
                     /locus_tag="PLU36_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HMZ46851.1"
                     /translation="MFHQLLQQIQQGNIKSLARCISLIENEVDGYELFLKQLPYSTTK
                     IIGITGPPGAGKSTLTDALISEIIAQQKKVAVVCVDPSSPFNLGALLGDRIRMSEWYN
                     NENVFIRSLATRGSLGGLHPKIIEITDILKAAPFDYIIVETVGVGQSEIEIVGLADTT
                     IVVVVPEAGDEVQTMKAGLMEIADIFVVNKADRPDADLFVKNLRQMLAPSFSNKQGEI
                     EVLKTIASQKKGVDILFKKIQQHATENNKEKHYLLLAEKAYHLIQQKRMKNIDKRMLK
                     EQIEKSTENNMYKFIEQFF"
     gene            2474..3178
                     /gene="trmB"
                     /locus_tag="PLU36_08620"
     CDS             2474..3178
                     /gene="trmB"
                     /locus_tag="PLU36_08620"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HMZ46852.1"
                     /translation="MGQKKLIRFNAIKTFNNVLEHPENMQGQWHKFFKNNHTITLELA
                     CGKGEYTVGLARMYVNRNFIGIDIKGNRIWRGAKTAMDEGLNNVAFLRTEIDQITQYF
                     SENEINEIWITFPDPQLRLSKAKKRLTHPKFLRLYQQILQPNGIIHLKTDSPNLYQFT
                     KTVIGLYNLELLTDYNNAYVENNIEEELKIKTHYEALDIAQSNKIHYLKFRINKNLPL
                     ALDNQLKSILHEQQND"
     gene            3159..3365
                     /locus_tag="PLU36_08625"
     CDS             3159..3365
                     /locus_tag="PLU36_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HMZ46853.1"
                     /translation="MSSKMIEGEDFYWENGLMVLTEKFHLSKGYCCGNGCKHCPYHYE
                     SVPEPKRTDLITLQKTSKSNPSKP"
     gene            3371..3736
                     /locus_tag="PLU36_08630"
     CDS             3371..3736
                     /locus_tag="PLU36_08630"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HMZ46854.1"
                     /translation="MTKTTITTNKNKSYSFFENVHEVVRLIPKGRVTTYGAIAKYLGV
                     KMSARMVGWAMNIAHTATPKVPAQRVVNRNGMLTGKIHFSTPTKMEELLKKEGINVIE
                     DKVVDFKNIFWDPSAELKW"
     gene            3911..>4300
                     /gene="accB"
                     /locus_tag="PLU36_08635"
     CDS             3911..>4300
                     /gene="accB"
                     /locus_tag="PLU36_08635"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HMZ46855.1"
                     /translation="MDLKQIHELIKIINKSNIGEISIEDKDGKVTIKQKEEQIVTVAA
                     PTPQVYNVAAPAAVAPAQATTTAPTAPAPAAVAPKADNLITIKSPMIGTFYRKSSPDK
                     PPFVEVGTDVAPGSVVCIIEAMKLFNEI"
BASE COUNT         1606 a          664 c          658 g         1372 t
ORIGIN      
        1 atatttatag gaataatttc tttatctcta tttatataat ttgccaaagc atacacttgc
       61 aatttatagc aatctcctaa aacgcctaaa ccaccagcca tgtcaccata taatgttcca
      121 tagcctgttg ctaattcact tttattgcta gtatttaaaa gaatataacc aaatttgttt
      181 gcaatggcca ttagtaaatt tcctctggta cggctttgta agttttcttc tgcaaggcta
      241 aaaggtaaat tattaaatat aggaattaat gtagaaagaa aactttcata aacattgtct
      301 attctaatta tatcataagg gttatttagg tttttactta gctgtactgc atcatttaca
      361 ctatgctcag tactatatgg cgatggcatt aaaactgctc ttacattttc ggcaccaaga
      421 gcttggcatg ctaaagttaa tactactgca ctatcaattc caccactact tcctagaatg
      481 gcttttgtaa aattcatttt agaaaaataa tctttaatcc ccaatactaa agcatcgtgt
      541 atttgagcaa tgtttaattg ttcatttaag gtagatggat ttaattcttt attagaaatt
      601 tgattagcca tttcaataac agatgcattg aaagttccat cattgttgag ttctacgcaa
      661 tctattgttt ctttaaaaac aggtaatgct ttaataagat ttgcattttt atcaaataca
      721 agacttgctc catcaaaaat aatttctgtt tgactaccta ctgtattgca ataaatcaaa
      781 ggtagtttgt atttcaaaac attggcttta ataattgctt ttctatcttc aatatgagtg
      841 taatcaaaag ggcttgcact taggttaatc attacatctg gctcttgctt cattaattca
      901 tccatggggc aaattctata caatggatta tcacccaaat tccaaatgtc ttcgcaaatg
      961 gttactgcta ttttttttcc tttaaaatct atggtattcc aattataagc aggttcaaaa
     1021 tatctgtatt cgtcaaacac atcatacgtt ggcagtaagg ttttatgtac ttctgctttt
     1081 atttcttttt catacaaaaa gaaagaagca ttgaataaat cttttccttc tttaatttta
     1141 tttcttgctg gtgagccaat taaaacacca atactatcag catgttcttt tattttgttt
     1201 attgcatggt aacatttatt aataaagtct tcaaactcta caaaatctct agcagggtag
     1261 ccacaaacac acatttcgct gaatacaatt aaatcgccac ctttttgttt agcctcttct
     1321 atggcctgaa taattttttg tgtattcaat tcaaaatttc caatatgata attttgttga
     1381 gctaaaaata ttttcatggt acaaatttca tatttataca aatacaaaaa agttatttaa
     1441 cagatagagt tactttaatg aaaatttaaa atagttgtaa gctatttatc ttttattgct
     1501 attctaagta aattcgtaca ttaaatacaa ttaccaaaaa tgtttcatca actattacaa
     1561 caaattcaac aaggcaatat taaatcttta gcacgttgta tttctttaat agaaaatgaa
     1621 gtggatggat acgaattgtt tttaaagcag ttgccttatt ctactacaaa aattattggc
     1681 attacaggcc cacctggtgc aggaaaaagt actttaaccg atgctttaat tagcgaaata
     1741 attgcacagc aaaaaaaggt tgctgtagtt tgtgtagatc cttcatcgcc ttttaattta
     1801 ggtgctttgt taggcgatag aattagaatg agtgaatggt ataacaacga aaatgtattt
     1861 atacgttctt tagcaacaag aggcagtttg ggaggcctac atccaaaaat tattgaaata
     1921 acagatattt taaaagctgc tcctttcgat tatattattg tagaaacagt gggcgttgga
     1981 caaagtgaaa ttgaaatagt aggattggca gatacaacca ttgtagttgt agtgccagag
     2041 gctggcgatg aagtgcaaac tatgaaggca ggcttaatgg aaattgcaga tatttttgtt
     2101 gtaaataaag cagataggcc agatgcagat ttatttgtaa aaaacttaag acaaatgttg
     2161 gctccttctt tttctaataa gcaaggagaa attgaagtac ttaaaaccat tgcctctcaa
     2221 aaaaagggag tggatatatt atttaaaaaa attcaacaac acgctacaga aaacaataaa
     2281 gagaaacact atttgttatt ggcagaaaaa gcatatcact taattcaaca aaaaagaatg
     2341 aaaaatattg ataagagaat gctgaaagaa caaatagaaa aatcaactga gaataatatg
     2401 tataaattta ttgaacaatt tttttagctg taagccatta ctagaaattc ttaattatat
     2461 cttcgccaat tctatgggac aaaaaaaact cattcgtttt aatgctatta aaactttcaa
     2521 caatgttttg gaacatccag aaaatatgca agggcaatgg cataaatttt ttaaaaacaa
     2581 ccatactata acactagagt tggcatgtgg taaaggagaa tacactgttg gcttagcaag
     2641 aatgtatgtt aatagaaatt ttataggaat agatattaaa ggaaatagaa tttggcgtgg
     2701 ggcaaaaaca gctatggatg aaggccttaa caatgtagct tttttacgaa ccgaaattga
     2761 ccaaataacg caatactttt ctgaaaatga aatcaatgag atttggatta catttccaga
     2821 tcctcaactt cgtttaagca aggcaaaaaa acggctaacg catcctaagt ttttaagatt
     2881 atatcagcaa attttacaac ccaatggcat tatacattta aaaacagata gcccaaacct
     2941 ttatcaattt acaaaaacag taattggttt atataattta gaactgctta cagattataa
     3001 taatgcttat gtagaaaata atattgaaga agaattgaaa attaaaacac attatgaggc
     3061 tttagatatt gcacaaagca ataaaattca ttatttaaaa tttagaataa ataaaaactt
     3121 accacttgca ttagacaacc aattaaaatc tattttacat gagcagcaaa atgattgaag
     3181 gagaagattt ttattgggaa aatggattaa tggttttaac agaaaagttt catttaagca
     3241 aagggtattg ctgtggtaat ggttgtaaac actgccccta tcattatgaa agtgttccag
     3301 aaccaaagag aaccgattta attactttac aaaagacttc taagagtaat ccatctaaac
     3361 cataagcaac ttgacaaaaa ctactataac aacaaataaa aacaaatcct attcattttt
     3421 tgaaaatgta cacgaagtag tacgtttaat acccaaaggc agagtaacaa cctatggggc
     3481 aattgcaaaa tatttaggtg taaaaatgag tgcaagaatg gtaggttggg caatgaatat
     3541 agcacatact gctacaccta aagtacctgc acaaagagtt gtaaatagaa acggaatgct
     3601 aacaggcaaa atacactttt ctacaccaac caaaatggaa gaattattaa aaaaagaagg
     3661 aataaatgtt atagaggata aagtagttga ctttaaaaat attttttggg atccttcggc
     3721 agagctaaag tggtaaattg tttaaattag caagtacata aaaatctatc attttttaag
     3781 aaaaaaggca acttttaatt aaaaacacgc aattttgatg aaattttacc tattttgctt
     3841 tcttgcttaa caaactaact aagaaagttc taacaaaaac aaaacacatt aacctcctaa
     3901 aactagcttt atggatttaa aacaaattca cgaactgatt aaaattatta ataaaagtaa
     3961 tattggagaa attagcattg aagataaaga tggtaaagta actatcaaac aaaaagaaga
     4021 acagatagtt acagttgcag caccaacacc tcaggtttat aatgttgcag caccagcagc
     4081 agttgcacct gcacaagcta caacaactgc accaactgca ccagcaccag cagcagttgc
     4141 acccaaggca gataatttga ttactataaa aagcccaatg attggcactt tttacagaaa
     4201 atcttctcca gataaacctc catttgtaga agtaggaaca gacgttgcac caggctcggt
     4261 agtttgtatt atagaagcaa tgaaactgtt taacgaaatt
//
LOCUS       DAOQPK010000190         4293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_13383_length_4293_cov_4.451628, whole genome shotgun sequence.
ACCESSION   DAOQPK010000190 DAOQPK010000000
VERSION     DAOQPK010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13383_length_4293_cov_4.451628"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..548
                     /locus_tag="PLU36_08640"
     CDS             108..548
                     /locus_tag="PLU36_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46856.1"
                     /translation="MAAKSDKGKKSVKPETKEIKESKKTSSKPKKQLEEEDEEDNFED
                     DLDSPSTSKKSSKKTPSKKSKEEDDDDDDDDVEDKDDWEKVEEEENWDPDFDEFDIPK
                     AKGGKGGSKKGDDDDFDFNEDFKEFDDDIFEDDEDDDYNSKDDF"
     gene            560..1852
                     /locus_tag="PLU36_08645"
     CDS             560..1852
                     /locus_tag="PLU36_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HMZ46857.1"
                     /translation="MKYYKTSFIIKNIKEFKQQMLSWGNQFNICCFLDNHQYQNNPTN
                     TECLLAVNPVKEIKVQNNYLDALTNFIQSNSGEWFFGHVSFNLKQEIYGIHSTKINQV
                     NFAPLYFFIPEIVVELKQQKLTIYSKHNNTDTIFSDILNINFKKKDTNTNASSFLIKP
                     KISKEEYIKCIHQLKQHIQRGDCYEINFCQEFFAKDVAINTIEVYKKLTTLSATPFAC
                     YYKLNEQYLLCASPERFVCKNGNKIISQPIKGTIKRNLKDSLQDDVLKKQLQNSSKDK
                     TENVMIVDLVRNDLSKICTKGSVIVEELFGVYSFPQVHQMISTVSGNVQKNINFSEVL
                     QATFPMGSMTGAPKKRVLELTEQYEKSNRGIYSGTVGYITPENNFDFNVVIRSIMYNA
                     VNKYLSYMVGGGITINSNAEDEYQECLLKATAIKEVLT"
     gene            2033..3046
                     /locus_tag="PLU36_08650"
     CDS             2033..3046
                     /locus_tag="PLU36_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HMZ46858.1"
                     /translation="MFTIQQELEKRILIIDGAMGTMIQRYKLQEEDYRGTRFINWHKD
                     VKGNNDLLSITKPAVITAIHKEYLLAGADIIETNTFSSTSIAQADYDMQEFAYELNVA
                     SATCAKNAINEYIKETNTTTPKFIAGAIGPLNKTLSLSPDVNNPGFRAVTFDEVVAAY
                     TEQIKGLIDGGVDILLIETIFDTLNAKAAIFAAKKFFRENNIEKPIMISGTITDASGR
                     TLSGQTLEAFYISVKHANPLSIGLNCALGANEMRGHIEELSNIASCYVSAYPNAGLPN
                     AMGEYDEAPEETAHFIEDWAKNKWVNIVGGCCGTTPEHIKHIAEYVKLLEPRSLPVLE
                     TTI"
     gene            complement(3088..3717)
                     /locus_tag="PLU36_08655"
     CDS             complement(3088..3717)
                     /locus_tag="PLU36_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HMZ46859.1"
                     /translation="MQSYLKLLLENKSWAKEKVEVDPDFFTNLAKLQTPEFLWIGCSD
                     SRVSADQITGTQPGEIFVHRNIANLVVNTDVNLMAVLDYAINHLKVKHVIVCGHYGCG
                     GVKASMCKTDLKYILNMWLRNIKDIQRIYEEELNNIADEEKRYDRLVELNVKEQVLNL
                     AKSSIIQNAWKGGDIPHLHGWVYSLNDGIIKPVFEMKAGTKINSIYEFE"
     gene            3886..>4293
                     /locus_tag="PLU36_08660"
     CDS             3886..>4293
                     /locus_tag="PLU36_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HMZ46860.1"
                     /translation="MKLTLDTNIVTDDFFNETILLGITTTIKNYKLCLLLNTFMGFHF
                     KLNNNIEICISKRKRKYYFSVYESVLKNSTLVHYLYHNHNDGEYLLPEFKHIDFLWLM
                     KGDILDTTQVEEIKTAIKTLNGVQMVAELTNEQI"
BASE COUNT         1715 a          591 c          656 g         1331 t
ORIGIN      
        1 tttgtttaaa aataaaataa aaaaaataga aaaaaattaa agaaaatttt ggttagtaat
       61 tttttgagtt gaaattttac aatccaatat taaaacttat tagaactatg gcagcaaaat
      121 ctgataaggg taaaaaatct gttaagcccg aaacaaaaga aattaaagaa tcaaaaaaaa
      181 cttcatcaaa acctaaaaaa caattagaag aggaagatga agaggacaat tttgaggatg
      241 atttagattc tccatcaacc tcaaaaaaat ctagtaaaaa aactccttcc aaaaaatcaa
      301 aggaagaaga tgacgacgac gatgacgatg atgttgagga taaagatgat tgggaaaaag
      361 tagaagaaga agaaaattgg gatcctgatt ttgatgaatt tgatattcca aaagcaaaag
      421 gaggaaaggg aggaagtaaa aaaggtgatg atgatgattt tgattttaac gaagacttta
      481 aagagtttga tgacgacatt tttgaagacg atgaagatga tgattataat agtaaagatg
      541 acttttaaca ttactaaaag tgaagtatta taaaacttcg tttataataa aaaatataaa
      601 agaatttaag cagcaaatgt tgagttgggg taaccagttc aacatttgtt gtttcttaga
      661 taaccatcaa tatcaaaata acccaaccaa tacagagtgt ttattagctg ttaatccagt
      721 taaggaaatt aaagttcaaa acaattattt agatgcgtta acaaatttca tccaatcaaa
      781 ctcaggagag tggttttttg gacatgtgag ttttaattta aaacaagaaa tatatggcat
      841 ccattcaacc aaaataaacc aagtaaattt tgctccttta tattttttta taccagaaat
      901 agttgtagaa ttaaaacaac aaaaacttac tatctatagc aagcataaca atacagatac
      961 catattttct gacatcctaa atattaattt caaaaagaaa gatactaata ccaatgcttc
     1021 cagcttttta attaagccaa aaatatcaaa agaagaatac ataaaatgta ttcatcaatt
     1081 aaaacaacat attcaaagag gcgattgcta cgagataaat ttttgtcaag agttttttgc
     1141 aaaagatgtg gcaattaata caatagaagt gtataaaaaa ttaacaacct tatctgctac
     1201 accttttgcc tgttattata aattaaatga acagtattta ctgtgtgcaa gccccgaaag
     1261 atttgtttgc aaaaatggaa ataaaataat ttctcaacca ataaaaggaa caataaaaag
     1321 aaatttaaaa gatagcttac aagatgatgt attaaaaaaa caattacaaa acagcagtaa
     1381 agataaaact gaaaatgtga tgattgttga tttagtaaga aatgatttaa gtaaaatttg
     1441 tactaaaggc tcagtaattg tagaagaact ttttggtgtt tatagttttc ctcaggtaca
     1501 tcaaatgata tctacagtta gtggcaacgt acaaaaaaat ataaattttt ctgaagtttt
     1561 acaagctact tttcctatgg gcagcatgac aggtgcccca aaaaaaagag ttttagaact
     1621 aaccgaacaa tacgaaaaaa gcaatagggg aatttatagt ggaactgttg gatatattac
     1681 acctgaaaat aattttgatt ttaatgttgt aataagaagt ataatgtata atgcagtcaa
     1741 taaatattta agctatatgg ttggtggtgg tataaccatt aacagcaatg cagaagatga
     1801 ataccaagaa tgtttattaa aggcaactgc cataaaagaa gtgcttacct aaaccacctt
     1861 ttacataaat aaaaaattac agtacatttg caaattgcag gcagatttgt ttattgttaa
     1921 gcctacatca aaacataact aataaacgaa ccaaccaata ttaccaatac aaaatatttg
     1981 gcctcgtttt ttagatacag tgggtgtagt acaaaaaaaa ttatacgtaa ttatgtttac
     2041 aattcaacaa gagttagaaa aaagaatatt aattattgat ggtgcaatgg gcaccatgat
     2101 tcaaaggtat aaattacaag aagaagatta tagaggtacc cgttttataa actggcataa
     2161 agatgtaaaa ggaaacaatg atttactaag cattaccaag ccagcagtaa ttactgctat
     2221 acataaagaa tatttacttg caggagcaga tataatagaa acaaatacat ttagtagtac
     2281 aagcatagca caagcagatt atgatatgca agaatttgct tatgaattaa atgtagcatc
     2341 tgcaacctgt gcaaaaaatg caattaacga gtatattaaa gaaaccaata caactacacc
     2401 taaatttatt gcaggtgcaa ttgggcctct taataaaaca ctaagtcttt cgcctgatgt
     2461 aaataaccca ggctttagag cagtaacttt tgatgaagtg gtagctgctt acacagaaca
     2521 aataaaaggt ttaatagatg gtggagtaga tattctttta attgaaacca tatttgatac
     2581 cttaaacgct aaagcagcaa tttttgctgc aaaaaaattt ttcagagaaa ataatattga
     2641 aaaacccata atgattagtg gaactattac tgatgcaagt ggtagaaccc ttagtgggca
     2701 aaccttagaa gcattttata tttcggtaaa acatgcaaac cctttaagta ttggtttaaa
     2761 ctgtgcacta ggtgccaatg aaatgagagg gcatatagaa gagctttcaa atattgcttc
     2821 ctgctatgta tctgcatatc caaatgcagg cttgccaaat gctatgggtg agtatgatga
     2881 agctccagaa gaaacagcac actttattga agattgggca aaaaataaat gggtaaatat
     2941 tgtaggtggt tgttgtggca caacaccaga gcatattaaa catattgctg aatatgtaaa
     3001 actattagag ccaagaagtt taccagtttt agaaacaact atttaataaa gtttccgcta
     3061 aatatagaat ggctaataag cttattatta ttcaaactca taaatagagt taatttttgt
     3121 tcctgctttc atttcaaaaa caggtttaat aataccatca tttaagctat aaacccaccc
     3181 atgaagatgt ggtatatcgc cacctttcca tgcattttga ataatagaag atttggctaa
     3241 atttaatact tgttctttaa catttagttc aaccaacctg tcatagcgtt tttcttcatc
     3301 agcaatatta ttcaactctt cttcgtatat tctttgtata tccttaatat ttcttaacca
     3361 catgtttaaa atatatttca aatcagtttt acacatagat gcttttacac caccacaacc
     3421 ataatgccca caaacaatta catgttttac ttttaaatga ttaattgcat aatctaaaac
     3481 tgccattaaa ttaacatcgg tatttactac taaattggca atatttctat gtacaaaaat
     3541 ttcgccaggt tgtgtaccag taatctgatc ggcagataca cgactatcgc tacaaccaat
     3601 ccacaaaaac tctggtgttt gaagtttggc aaggttggta aaaaaatctg gatccacttc
     3661 taccttttcc tttgcccatg atttattttc taaaagcagc tttaggtaag attgcacttg
     3721 agttgtttta taaagttttc aaataaaaat aatttgtatc aaaaaaaatt acctgtaact
     3781 atatttaatt tatacaaatg taaccttaat aaaatttaaa aattattatg taccatttaa
     3841 tttataaatt aatattttta ttgaacaaaa gggtagccat taattatgaa gttgacacta
     3901 gatacaaata ttgtaactga cgattttttt aatgaaacaa tattattagg aattaccaca
     3961 accattaaaa actataagct ttgtttactt ttaaatactt ttatgggttt tcattttaag
     4021 ttgaataata atattgaaat ttgtatttct aaacgcaaaa gaaaatatta ttttagcgta
     4081 tatgaatctg ttttaaaaaa cagcacatta gtacattact tataccataa ccacaacgat
     4141 ggagaatatt tattacctga atttaaacat atagattttt tgtggttaat gaaaggagat
     4201 attttagata ctacacaggt agaagaaata aaaactgcta ttaaaacatt aaatggtgta
     4261 caaatggtgg cagagctaac caatgagcaa ata
//
LOCUS       DAOQPK010000191         4264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_13519_length_4264_cov_3.938228, whole genome shotgun sequence.
ACCESSION   DAOQPK010000191 DAOQPK010000000
VERSION     DAOQPK010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13519_length_4264_cov_3.938228"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PLU36_08665"
     CDS             complement(<1..342)
                     /locus_tag="PLU36_08665"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HMZ46861.1"
                     /translation="MPLITLATDIGQQDYIVGAFKGQLHAEISQLSIADISHYLPLDN
                     FAHAAYICSNAYKYYPSNTLHILLINVFDEPETHFVICNYNNQFILCPNNGILSMLVK
                     TLPENTTVLPII"
     gene            complement(372..1178)
                     /locus_tag="PLU36_08670"
     CDS             complement(372..1178)
                     /locus_tag="PLU36_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ46862.1"
                     /translation="MENFTQKVIVITGGSDGIGKALVEAFLKLGAKVATCGRNYDKLY
                     QLQSSHTGMPLLIHTADVSNEQECINFIHTVVKNFGTIDILINNAGVSMRSLFEDVEI
                     EALRKVVDVNLWGTVNCTKYALPYILKSKGSIVGISSIAGFRGLPGRTGYSASKFAVN
                     GFLEALRTELLHSGVNVMWICPGFTASNIRRAALNYKGQAHGETSMDEGKMMSAEDCA
                     THIIKAIYKRKRTLILTNTGKKTVWLNKFLPALADKFTYNYFFKNGKLFK"
     gene            complement(1314..3800)
                     /locus_tag="PLU36_08675"
     CDS             complement(1314..3800)
                     /locus_tag="PLU36_08675"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HMZ46863.1"
                     /translation="MTNKRLITLLAFIFIAAVVYAQPKANYRLASKYSPKKLDKIVYS
                     LNVDPHWLKNGNRFWYVYETNQGKKWYIVDANKGEKKYLFDNDKMAAEISKIVKDPFD
                     AQNLGLDSIRFIGDETKFQFEVKSTEEIEKKDTTAKDKKDAKPIKEKKVFYFEYDINT
                     GLVTELQGFKKPKRKPFWANMSPDGNTIVFGKHHNLFYMDKANYEKALLNEKDSTIVE
                     HPLTTDGEEYFSYYGGGAYSGNGENNVEKEKNKDDRKPVFIMWSPDSKHFALTKSDAR
                     KVKELWVINSAADPRPTIETYKYWMPGEKESPKDYMMVFNMNTKKGKEVKVSNFKDQD
                     LNLYSQPSLQNTRDDMFKPFLWLGTNTQFYFSRTSRDLKKIDQCLYDISADSVKVLIE
                     ERMNTSIEIRRPGLVNNGKELIEWSERDGWAHFYLYDENGKLKNQITSGPWHCENIIN
                     IDEKKRVLYFTANAKEKNENPYYLHLYKVNFDGTGLKLLNEGDFDHQISLNDNNAFFV
                     NNFSRVNTTPKSVLYNNEGKKVLDLETADLSSLFATGYKFPEPFKVKADDGITDLYGV
                     MYKPFDFDSTKKYPLIEYVYPGPQTEAVNASFSRGMDRVDRLAQLGFIVVTVGNRGGH
                     PSRSKWYHNFGYGNLRDYGLADKKATAEQLADRFSFIDINKVGIHGHSGGGFMSTAAM
                     LVYPDFFKVAVSNAGNHDNSVYNRWWSEKHHGVKEIISAKGDTSFAYSIDKNPDVAKN
                     LKGRLLLIHGEIDNNVHPANTMRVVNALIKANKRFDMLLLPTQRHAFGNMTEYWFWKT
                     ADYFTYWLMGDKTDHEVDIEEINREIEKKK"
     gene            complement(3897..3968)
                     /locus_tag="PLU36_08680"
     tRNA            complement(3897..3968)
                     /locus_tag="PLU36_08680"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3933..3935),aa:Arg,seq:ccg)
BASE COUNT         1361 a          730 c          575 g         1598 t
ORIGIN      
        1 aataataggt aaaacagttg tattttctgg caatgtttta accagcatac ttaggatgcc
       61 attgttagga caaagaataa attgattatt ataattgcaa ataacaaaat gagtttcggg
      121 ttcatcaaaa acatttatta gtaaaatatg cagtgtgttt gaaggataat atttataagc
      181 attgctacat atatacgcag catgtgcaaa gttatctaat ggcaaatagt gtgaaatatc
      241 tgcaatagat agttgtgaaa tttctgcatg taattgccct ttaaaagcac caacaatata
      301 atcttgttga ccaatatctg ttgcaagtgt tattaaaggc atattttaag atagaaataa
      361 agttgaattg gttatttgaa taattttccg tttttgaaaa aataattata ggtaaactta
      421 tctgctaaag ctggtaaaaa tttatttaac cacacagttt ttttacctgt gtttgttaaa
      481 attaaagttc gttttctttt atagattgct ttaattatat gcgttgcaca atcttcggca
      541 ctcatcattt taccttcatc catagatgtt tcgccatgag cctgcccttt atagtttaat
      601 gctgcccttc taatattgga agctgtaaag cctgggcaaa tccacattac attaacacca
      661 gagtgtaaaa gttctgttct taatgcctct aagaaaccat taactgcaaa tttagatgct
      721 gagtaaccag tacgccctgg caagcctcta aagcctgcaa tagaagatat accaactata
      781 gagccttttg attttaaaat gtatggcagt gcatatttgg tacaatttac agtaccccat
      841 aaattaacat ctacaacttt tcgtaaagcc tctatttcta cgtcttcaaa taatgacctc
      901 atagaaactc cagcattgtt aatcaaaata tcaatagttc caaaattttt tacaacagta
      961 tgaataaagt ttatacattc ttgttcgtta cttacatctg cagtatgtat taaaagaggc
     1021 attcctgtgt gtgaagattg caactgatac agtttatcat aatttcttcc acatgtagcc
     1081 actttggctc ctaattttaa aaatgcttcc actaaagctt ttccaattcc atcagaaccg
     1141 cctgttatta caattacttt ttgggtaaaa ttttccatgc aaaatcttta taaataatta
     1201 ttgcaaaaat aagctgttag gtatgagttt tattttaaag tttgaataaa ttttaattaa
     1261 aaaaggttgc attaaaacta tgcaaccttt tagaaagaaa tagcctggtt aggttatttc
     1321 ttcttttcaa tttctctgtt tatttcttca atatctactt catggtcagt tttgtctccc
     1381 attagccaat aggtgaagta atctgcagtt ttccaaaacc aatattctgt catattacca
     1441 aaagcatgtc gttgtgttgg taataataac atatcaaatc ttttattagc ttttattaaa
     1501 gcatttacaa ccctcattgt gtttgctgga tgtacgttgt tatcaatttc tccatgaatt
     1561 aataataatc ttccttttaa gtttttagca acatctgggt ttttatcaat actatatgca
     1621 aagcttgtat ctcctttagc actaataatt tcttttactc catgatgttt ttcactccac
     1681 caacgattgt aaacactgtt atcgtgatta cctgcattgc ttacagcaac tttaaagaaa
     1741 tctggataaa ccaacatggc agcagtactc ataaaaccac caccactatg gccatgaata
     1801 cctactttgt ttatgtcaat aaaagaaaat ctatctgcta attgttcagc agttgctttt
     1861 ttatcggcta agccataatc tcttaaattt ccataaccaa agttgtggta ccatttactt
     1921 ctggatggat gccctcctct attaccaaca gtaactacaa taaaaccaag ttgtgctagt
     1981 ctatccactc tatccattcc tctactaaaa cttgcattta cagcttctgt ttgtgggcca
     2041 gggtaaacat attcaatcaa tggatatttc tttgtgctat caaaatcaaa aggcttatac
     2101 attacaccat ataagtctgt aataccatca tctgctttaa ctttaaatgg ttctggaaat
     2161 ttataccctg tagcaaataa cgagcttaaa tctgctgttt ctaaatctaa tacttttttg
     2221 ccttcgttgt tatataaaac agattttgga gtagtgttta ctctcgaaaa attattgaca
     2281 aaaaaagcgt tattatcatt caggctaatt tggtgatcaa aatctccttc gtttaataat
     2341 tttaaaccag taccatcaaa atttactttg tataaatgaa ggtagtaagg gttttcattt
     2401 ttctcttttg cattagcagt aaaatacagc actctttttt tctcatcaat attgattata
     2461 ttttcacaat gccatgggcc actcgtgatt tggtttttta attttccatt ttcatcgtac
     2521 aaataaaaat gagcccagcc atctctttcg ctccactcaa ttaattcttt accattatta
     2581 acaagtcctg gtcttcttat ttcaatggag gtgttcattc tttcttcaat aagaacttta
     2641 accgaatcgg cagagatgtc gtataaacat tggtcaattt ttttaaggtc tctacttgtt
     2701 ctgctaaaat aaaattgtgt attagttcct aaccataaaa atggtttaaa catatcgtct
     2761 cttgtatttt gcaaagatgg ttggctatac aaattcaaat cctgatcttt aaaattgctt
     2821 actttaactt cttttccttt tttagtgttc atattaaaca ccatcatata atctttagga
     2881 ctttcttttt ctccaggcat ccaatacttg taagtttcaa ttgttggtct tggatctgca
     2941 gcactgttaa ttacccataa ttctttcact tttctagcat cagatttggt aagtgcaaaa
     3001 tgtttactat ctgggctcca catgataaaa actggtttgc gatcatcttt atttttttct
     3061 ttttctacat tattttcccc attaccactg tatgcacctc caccataata cgagaaatat
     3121 tcttctccat ctgtagttaa agggtgttct acaattgtgc tatctttttc atttaataat
     3181 gctttttcat aattggcttt atccatataa aacaaattat gatgtttgcc aaaaacaatt
     3241 gtattgccat ctggcgacat attagcccaa aatggtttgc gttttggttt tttaaaccct
     3301 tgcaattccg ttactaaacc tgtattaata tcatattcaa aatagaaaac ctttttttct
     3361 tttatgggct tagcatcttt tttatctttt gctgtggtat cttttttctc tatttcttct
     3421 gtacttttta cttcaaattg aaatttagtt tcatcgccaa taaagcgaat gctgtctaat
     3481 ccaagatttt gagcatcgaa tgggtctttt acaattttag aaatttcagc agccatttta
     3541 tcgttatcaa aaagatactt tttttctcct ttattagcat caacaatata ccatttttta
     3601 ccttggttgg tttcgtaaac ataccaaaaa cggtttccat tttttagcca atgtggatca
     3661 acatttaagg aataaacaat tttatctaat tttttaggag aatacttaga agctaatcta
     3721 taattagctt ttggttgtgc gtacaccact gctgcaataa aaataaatgc aagtagtgta
     3781 ataaggcgtt tgttagtcat ggtttgaagt tttagaaagc ggaaaaataa caacaaaaaa
     3841 cagtaattaa ttaccattta taaaaaaact gaacttattt ttaaaaaaac tataagtggc
     3901 ctcgtcagga atcgaacctg aatcagaagc ttcggaaact tctgtactat ccattgtact
     3961 acaaggccat taaacaacaa aagtaaagat ttatggttca ggtttaattt tttgagcttt
     4021 caataaatag atttctataa taattaattt tttaaatagc atttaaaaat taacataaaa
     4081 cagtaatcta aaaatgtcac ttacagcaat gttttacata atgtattaaa tttgaataaa
     4141 aagtgaaatt ctttcaaatt ttaaaaagct gcatcgacaa ggtaatattt atctaatcag
     4201 tattcattat attttattaa agttttttat tttcattgta attcataagt attcaaatat
     4261 attc
//
LOCUS       DAOQPK010000192         4197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_13857_length_4197_cov_3.463544, whole genome shotgun sequence.
ACCESSION   DAOQPK010000192 DAOQPK010000000
VERSION     DAOQPK010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13857_length_4197_cov_3.463544"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="PLU36_08685"
     CDS             complement(<1..430)
                     /locus_tag="PLU36_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HMZ46864.1"
                     /translation="MASKGTVKQVIGAVVDVHFPNDNALPDIYNALELTRENGDKLVL
                     EVQQHLGEDSVRTIAMDGTEGLVRGMEVIDTGNAIKMPIGEAINGRLFNVTGDAIDGL
                     PQLNKSNGRPIHAKPPSFENLNTATEVLFTGIKVIDLIEPY"
     gene            763..1734
                     /locus_tag="PLU36_08690"
     CDS             763..1734
                     /locus_tag="PLU36_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46865.1"
                     /translation="MKFTNLKYVLLFAIIILLGSCFKETSYEKGANVYGFSSGTLKDS
                     LGDCKGIVIKGAYQAGTVLTDSNYIIVGVNITQSGKYVIYSDTVNGFWFRDSGYVAVG
                     TNTIKIKAFGTPVSNVNADFVVTYNNSVCLFSITAGIVAPLPIISDYFPRSIGSNWGY
                     NVLGLDSLHTKSTPKDTILGGNSYRVFYANGSLDDTAFYRKSGGDYYRWDALDGNTGY
                     MPLLFLKEDKPAGTQWESNIANTVYSGIPTQVKMKYTLMSVNVSRTVESNTFDSVIHV
                     KNELQYKILGTFQTIQTMNTYFAKNVGLIEFEAAGIYQQKLRRWKVY"
     gene            complement(1747..2394)
                     /gene="nth"
                     /locus_tag="PLU36_08695"
     CDS             complement(1747..2394)
                     /gene="nth"
                     /locus_tag="PLU36_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HMZ46866.1"
                     /translation="MNKKERYQFVIDYFQEFMPVAETELIYDNPYQLLVAVILSAQCT
                     DKRVNQTTPAIFKKYPHPQSLSEASFQDLFALIKSISYPNNKTKHLIGMAQMLMNKFN
                     GEIPLTVNELIQLPGVGRKTANVITSVIDNQPNMAVDTHVFRVSKRIGLVSPKANTPL
                     AVEKELIKNLPTELIYKAHHWLILHGRYICLARSPKCNECGIATACLYYAKLLKQ"
     gene            complement(2422..>4197)
                     /locus_tag="PLU36_08700"
     CDS             complement(2422..>4197)
                     /locus_tag="PLU36_08700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ46867.1"
                     /translation="GQPSSGNANASILATCANVPFNLNLTAFTSTKGISFQWQSSGTG
                     QNIWVNIPGADSNFVTTTQTNASDYRCIVTCTKSALNDTSNVLTVGQIAAIACTLEND
                     ECATATTITQNAYNNCTGTLFNTSLASLSANTSTFYTGSQEDDIWFKFIATNDKAVIR
                     FFDITAVSGTLGNMQFAFYTGTNCNTLTDLGGRTVQINNNEGEVLLYGLTLGNTYFVR
                     VATEGTNWRAIGKICIAEPAIAIGSNNNCISLTTTESTISNHNSWIPIYDGAKLVAEI
                     NAQGNTMGAITANLFTTDIVRTFAGNGRPYLSRNIQITPAIQPSSNVKVRLYILYAEI
                     TLLASNPSSLVNTINDLYITKNNDACLPNYSGAGTFIIPTARVNYANGGYIEFETSSF
                     SSFYLHGGLGVLPITIQYFNGKKDGNYNLLQWQTINELHTTGFEVQRSTDGKYFSTIA
                     MVPTKATNNNIDKTLQYSFNDYKPLFNSYYRLKQTDKNGAVSYSNIVFISSAFINQTQ
                     LIGIYPNPVKDVCKVLLFIPTTTNISLVLTDITGKIITQKNTTYLQGNIEEKISMGQL
                     LNGIYLLKITDINGNLISIQKIVKQ"
BASE COUNT         1347 a          625 c          706 g         1519 t
ORIGIN      
        1 cataaggctc aattaggtca attactttaa tacctgtaaa tagtacttct gttgctgtat
       61 ttaaattttc aaaacttgga ggtttagcat gaatgggtct tccattgctt ttatttaatt
      121 gtggtaaacc atcaattgca tctcctgtaa cattaaataa acgaccatta atagcttcgc
      181 caattggcat tttaatggca ttgcctgtat caattacttc cataccacgt actaaacctt
      241 ctgtaccatc cattgcaata gtacgtacac tgtcttcgcc taagtgttgt tgaacttcta
      301 acactaattt atccccattt tctcttgtaa gttcaagagc attgtaaata tcgggcaatg
      361 cattatcgtt tggaaagtgc acatctacta ctgcacctat cacttgttta actgtacctt
      421 tagaagccat aattaattat gatttttgta tttggcggca aaggtaaggg gtaacaatct
      481 ttcaaaaaac taaatatacc atgtttttaa aggaattttt atagtgtgaa taagtacgga
      541 aaaatagtat ttatagcttt ttcataataa tacctgtata ttaaccctaa cttttatata
      601 atgattatag tttttattaa tttaatttta ggttaggcag caagtaatta ctaaggttta
      661 tctaaaaact tgaataatta tagctacata ttcggttttg aaatattttt ggaacaatat
      721 ttttttaata aaacagtttt ttacaatcag aaaaacagat aaatgaaatt tactaacctt
      781 aaatatgtat tgctatttgc aataattatc cttttaggct cttgttttaa agaaacaagt
      841 tacgaaaaag gggctaatgt gtatggcttt tcttcgggta ctttaaaaga cagtttggga
      901 gattgtaaag gaatagtaat aaaaggtgct taccaagctg gtactgtttt aacggatagt
      961 aattacatta ttgtgggagt taatattaca caatcgggta aatatgttat ttattctgat
     1021 acggtaaatg gattttggtt tagagatagt ggctatgttg ctgttggcac taatactatt
     1081 aagataaaag catttggcac tcctgtaagc aatgtaaatg ccgattttgt tgtaacttac
     1141 aataactcgg tttgtttatt ttcaataact gcaggcattg ttgctccttt accaattatt
     1201 agcgattatt ttccaagatc tataggtagt aactggggtt ataatgtgtt aggcttagat
     1261 tcgttacata ctaaatctac gccaaaagat actattttag gaggaaattc ttatagggtt
     1321 ttttatgcca atggtagttt agatgatact gcattttacc gaaaatctgg tggagattat
     1381 tataggtggg atgctttaga tggcaatact ggatatatgc ctttattgtt tttaaaagaa
     1441 gataaaccag caggcaccca atgggagagt aatattgcta atacagtata tagtggcatt
     1501 cctacacaag taaaaatgaa atatacctta atgagtgtaa atgttagcag aactgttgaa
     1561 agcaacactt ttgatagtgt aattcatgta aaaaatgaac tgcaatataa aattttagga
     1621 acttttcaaa ccatacaaac tatgaatacc tattttgcca aaaatgtagg gctaattgaa
     1681 tttgaggctg cgggtattta tcagcaaaaa ttaagaaggt ggaaagtgta ttaaactata
     1741 aaatgtttat tgctttaata gtttagcata atacaagcag gcagtggcaa taccacattc
     1801 gttacatttt ggagaacgtg ccaaacaaat atatcttccg tgtaaaatta gccaatggtg
     1861 tgctttataa attagttcgg taggtaagtt tttaatcaat tctttttcta cagctaaagg
     1921 tgtatttgct tttgggctaa ccaagcctat acgtttacta accctaaaca catgtgtatc
     1981 tactgccata ttaggttggt tatctataac actggtaatt acgttggctg tttttcttcc
     2041 tactcctggt aattgtatta attcatttac agttaaaggt atttctccgt taaatttatt
     2101 cataagcatt tgtgccatgc ctattaagtg tttagtttta ttgttggggt aagaaatact
     2161 ttttatcaaa gcaaacaaat cttgaaaact ggcttcagac aaggattgtg gatgaggata
     2221 tttcttaaaa atggcaggcg ttgtttggtt taccctttta tctgtacatt gagccgataa
     2281 aattactgct actaataatt gataaggatt atcgtaaatc aattctgttt ctgccaccgg
     2341 cataaactct tgaaagtaat caattacaaa ttggtatctt tcttttttat tcattctata
     2401 taaaatagta atttttttta cttattgttt tactattttt tgaatgctaa tcaggttgcc
     2461 attaatgtct gtaattttta acaagtatat accatttaat aattgcccca tacttatctt
     2521 ctcttctata ttgccttgta agtaagtagt atttttttgt gtaataattt tgcctgtaat
     2581 atctgttaaa actaaagaaa tattggtagt tgttggaata aataatagca ctttacatac
     2641 atcttttaca gggtttggat aaatgccaat taattgtgtt tggttaataa aggcagatga
     2701 aatgaataca atatttgaat aagaaacagc cccattttta tctgtttgtt ttagtctgta
     2761 atagctatta aataaaggtt tgtaatcgtt aaagctatat tgtaaagttt tatctatatt
     2821 gttgttggtt gctttggtag gtaccattgc aatggtagaa aaatattttc catcagtact
     2881 tctttgtact tcaaatccag tagtatgtaa ttcgtttatt gtttgccatt gtaataaatt
     2941 gtagttgcca tctttttttc cattaaaata ttgaatggta attggtaaaa cacctaagcc
     3001 tccatgtaaa taaaaagaag agaaggaaga agtttcaaat tcaatataac caccattggc
     3061 ataatttacc cttgcagtag gtattataaa cgtgcctgca ccagagtaat tgggtaaaca
     3121 tgcatcgttg tttttagtta tgtacaaatc gttaatagta tttactaaag acgatggatt
     3181 agatgctaaa agtgttattt cggcatataa aatatataat ctaactttta cattactact
     3241 gggttgtatg gctggtgtta tttgtatgtt tctacttaaa tagggcctac cattgcctgc
     3301 aaatgttcta acaatatcgg tagtaaataa attggcagtt atagcaccca ttgtatttcc
     3361 ttgagcattt atttcggcta ctaattttgc tccatcataa ataggaatcc agctattatg
     3421 gttgctaatt gtactttctg ttgtagttag cgaaatgcaa ttattattac taccaatagc
     3481 aattgctggt tctgcaatac aaatttttcc tatggctctc cagtttgttc cttctgtggc
     3541 aactcttaca aaataagtgt tgccaagggt taaaccatat agcaaaacct ctccttcgtt
     3601 gttattaatt tgtacagtgc gtccgcctaa atcagttaat gtattacaat ttgtacctgt
     3661 ataaaaagca aattgcatat tacccaatgt gccactaact gcagtaatat caaaaaatct
     3721 tataacagct ttatcgttag ttgcaataaa tttaaaccaa atatcatcct cctggcttcc
     3781 tgtataaaag gtagaggtat tagcagataa actagcaagg ctggtattaa ataaagtacc
     3841 agtacaattg ttataggcat tttgtgtaat agttgtggca gttgcacatt catcattttc
     3901 aagagtacaa gcaattgcag caatttgccc aactgtaagt acattactgg tatcatttaa
     3961 agccgatttg gtacaagtaa caatacatct ataatcactt gcattggttt gagtagttgt
     4021 tacaaaatta gaatcggctc caggaatatt tacccaaata ttttgccctg taccagaaga
     4081 ttgccactga aaagaaatac cttttgtgct tgtaaaagca gttaagttaa gattgaatgg
     4141 cacattggca caagtagcaa gaatagatgc attggcatta ccactactag gttgccc
//
LOCUS       DAOQPK010000193         4188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_13903_length_4188_cov_4.678684, whole genome shotgun sequence.
ACCESSION   DAOQPK010000193 DAOQPK010000000
VERSION     DAOQPK010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13903_length_4188_cov_4.678684"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..903
                     /locus_tag="PLU36_08705"
     CDS             193..903
                     /locus_tag="PLU36_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46868.1"
                     /translation="MFHKSLFYIFIIILSINVFTTNAFWGKGYKPYSKNTTYDNLFVI
                     SANDFNDDILLRIDNATNTTSNIKVKKTENGTYTDSTSTISVGDEGMKEVTFSQNWGQ
                     FQGGSGTGVYEMNFSFGDDEANYKATVSYATITPSISAVGPIKATGIPQSIISETNPQ
                     SVIQVEEGSTIEPMCQKSTALYKKLSTGYGKGDKLEEKLNGGSVNDYTLALMLNPPFM
                     SSCSFFLCHPERSEGSKG"
     gene            complement(1088..2422)
                     /locus_tag="PLU36_08710"
     CDS             complement(1088..2422)
                     /locus_tag="PLU36_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMZ46869.1"
                     /translation="MKHILTIFFSIIILNAFSQETVYPAKIQTETIALTNATIHIGNG
                     DIIENGMIVFSKGKIIDIRPTAPIADIKVIDCKGKHIYPGLITPHTDLGLNEINSTRA
                     MLDVRELGEINANIRSIIAYNSDSKVIGTLRSNGILFANIVPQGGIISGSSSVVQLDA
                     WNWEDAAYKTDGGIHFRVPNLTPRRAFWFMPAVNNTNTAYERIENVRKFFNEAKAYYA
                     IAKPENTNLKFEAVKGLFTEIDAYPNTNWKGVKKALRPQQNFFIHAETVKEIQIGVEL
                     AKEFQFKATIIGGTDSWMIADYLKENNVAVILNQMHSLPVIQDDDIDQPYKTPYLLQK
                     AGVLFAIGDVDANTKGRNLLFNAGTSAAYGLTKEQALQAITLNAAKILGIEDKTGSLE
                     KGKDANIVVSEGDILDMKSSNVVYAFIQGREVSLDNKQKQLYERYKYKYGIK"
     gene            complement(2475..>4188)
                     /locus_tag="PLU36_08715"
     CDS             complement(2475..>4188)
                     /locus_tag="PLU36_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMZ46870.1"
                     /translation="YEATIKGNSEKPTLTLKPYEDSVNIPTTLTLNNQLVKINWSAKA
                     DKGKQNNLSGIIGSDAWAGNGYLANGDVVIWKLFAKKENEGKSAKVDTSNKNTTHSTD
                     KTAITINDVVYPFSGYGYKKEFAPMQEKILIRNVTVWTNEKDGKLENTDVIINKGKIE
                     RIGKHLPVTLDMSWKTIDGTGKHLTAGIIDEHSHIAITGGVNECTNAVTSEVRIGDIL
                     DPEDINIYRQLSGGVTTSHLLHGSCNPIGGQTQLIKLRWGKGAEELKFQGSDGFIKFA
                     LGENVKSSRSTNNTRFPDTRMGVEQVYADAFQRAIDYKQLPVGKRRDLQLDALVEILN
                     NTRFITCHSYVQSEINMLIKVAEKYNFKINTFTHILEGYKVADKMKKNGINASTFSDW
                     WAYKLEVQDAIAYNAAIMQKMGLNVAINSDDAEMARRLNQESAKSIKYGGVSEEEALK
                     MATLNPAKMLHIDNKVGSIKVGKDADVVLWNDHPLSIYAKTLYTIVDGVIYFDREKDE
                     ELRKNIAQEKSRLIQKLLAEKKKPFNAGKMQPARPRMEVINECEEDHKHNHSIFERED
                     EFSY"
BASE COUNT         1256 a          728 c          613 g         1591 t
ORIGIN      
        1 taaagaagca agcgggcaaa caatatgcct ttatgatgca gtagtaaagc aaaacggaac
       61 agatgtagta aatgattaat attttattaa ttaaagaata gcactttgta tatacaaagt
      121 gctttttatg tgtataacta taatactatt ataaatatta tgatacaata atgctataac
      181 aaatacacaa ttatgtttca caaatcatta ttttatattt tcattattat attatcaatt
      241 aatgtgttta ctacaaatgc cttttggggc aaagggtata agccatatag caaaaacact
      301 acttatgaca atttatttgt aatatctgct aatgatttta atgacgatat attgcttcgt
      361 atagataatg ctactaatac tacttctaat ataaaagtta aaaaaactga aaatggaaca
      421 tatacagatt ccacctccac tatatcagta ggcgatgaag gtatgaagga agtgacattt
      481 tcgcagaatt gggggcaatt tcaaggaggg agtgggacag gggtttatga aatgaatttt
      541 agttttggag atgatgaagc aaattataaa gcaacagtat cttatgctac tattacacct
      601 agtataagtg cagtaggacc aatcaaagca acaggaatac cacaatcaat tattagtgag
      661 acaaatccac aatctgtaat acaagtagaa gaaggtagca caatagagcc aatgtgtcaa
      721 aaatcaacag cattatacaa aaagctatca acaggatatg gtaagggaga taaattggaa
      781 gaaaagttaa atggtgggtc tgtaaacgat tatacacttg ctttaatgct gaaccctcct
      841 ttcatgtcat cctgctcctt ttttttatgt catccagagc gaagcgaagg atctaaaggt
      901 taagaataga gagattcctc gtaaactcgg aatgacagat actttttttt gtcatcctat
      961 tccccctttt ttgtcatcct gagcgaagcg aaggatctaa agattaagaa caaagagatt
     1021 cctcgcaagc tctgaatgac aatacatttt tatttcattt tattctacat aaaatggtat
     1081 ttattattta ttttatacca tacttgtact tgtatctttc gtagagttgt ttttgtttat
     1141 tatctaaact cacctctctg ccttgtataa aagcatacac cacattgctt gatttcatat
     1201 ccaaaatatc gccttcgctt acaacaatat tagcatcttt ccctttttct aaactacctg
     1261 ttttatcttc tattcctaaa atttttgctg catttagtgt tatggcttgc aatgcttgtt
     1321 ctttggttaa gccataagca gccgatgtgc cagcattaaa taatagatta cggccttttg
     1381 tattagcatc tacatctcca attgcaaaca acactcctgc tttttgtaac aagtagggtg
     1441 ttttataagg ttggtctata tcatcatctt gaataactgg taaactatgc atttgattga
     1501 gaattacagc tacattattt tcttttaaat aatctgcaat catccaacta tctgtgcctc
     1561 caattatagt tgctttaaat tgaaattctt ttgctaactc aacacctatt tgaatttctt
     1621 taactgtttc ggcatgaata aaaaagtttt gctgaggacg aagtgctttt tttacacctt
     1681 tccaattagt gttagggtac gcatctattt cagtaaataa tccttttact gcttcaaatt
     1741 taaggttagt attttctggt tttgcaattg cataataggc tttggcttcg ttaaaaaact
     1801 tccgaacatt ttctattctt tcataagcag tatttgtgtt gttaacagca ggcataaacc
     1861 aaaatgctct tcttggtgta agattaggta cacgaaaatg aattccacca tctgttttat
     1921 aagctgcatc ttcccagttc catgcatcta attgcacaac agatgaagag ccactaataa
     1981 tgcctccttg aggaacaata ttggcgaata aaattccatt gcttcttaaa gtgccaatta
     2041 ctttactatc gctattgtat gcaataatgc ttctaatatt tgcattaatt tcacccaatt
     2101 ctctcacatc taacatggct ctagtgctgt taatttcgtt taagcctaaa tcggtgtgtg
     2161 gtgttattag tccaggataa atgtgctttc ctttacaatc aattacttta atatctgcaa
     2221 ttggagctgt tggtctaatg tcaattattt ttcctttaga aaaaacaatc atgccatttt
     2281 ctataatatc tccattgcca atatgtatag ttgcattagt taatgcaata gtttcggttt
     2341 ggatttttgc aggatatact gtttcttgag aaaaagcatt taatataata atgctaaaaa
     2401 atatggttag tatatgcttc atataataat tttaaaatgt taaacaattc aattttttat
     2461 ttatgaatta aggattaata gctaaactca tcttctcttt caaaaatgct gtgattatgt
     2521 ttatggtctt cttcgcactc atttataact tccattcttg ggcgtgcagg ttgcatttta
     2581 cctgcattaa atggtttctt tttttctgct aataattttt gaatcagtct tgatttttcc
     2641 tgagcaatat tttttcttaa ctcttcatct ttttctctat caaaatatat aaccccatct
     2701 acaatggtgt acaatgtttt tgcataaatg cttagaggat gatcattcca taacacaaca
     2761 tcggcatctt tacccacttt tatgctacct actttattat ctatatgcaa catttttgca
     2821 ggattaagcg ttgccatttt taaagcttct tcttcgctaa cgccaccata tttaatagat
     2881 tttgcagatt cttgatttag cctacgagcc atttctgcat catcgctatt tatagctaca
     2941 tttagaccca ttttttgcat aatagcagca ttgtatgcaa ttgcatcttg tacttctaat
     3001 ttatatgccc accaatcgct aaatgttgat gcattgattc catttttttt cattttatct
     3061 gctaccttat atccttctaa aatatgtgtg aaagtgttta ttttaaaatt gtacttttcg
     3121 gcaactttaa taagcatatt tatttcgctt tgtacatagg aatggcaagt aataaatctt
     3181 gtattattta atatttctac caatgcatct aattgcaaat ctcttctttt gccaactggt
     3241 aattgtttgt aatcaatggc tctttgaaaa gcatcagcat acacttgttc tacacccatt
     3301 ctggtatcag gaaaacgagt gttattagta cttctggagc tttttacatt ttctcctaat
     3361 gcaaatttta tgaacccatc ggatccttga aattttaatt cttctgcacc tttaccccaa
     3421 cgtaatttta ttaattgtgt ttgcccacct attggattac agcttccatg taataaatga
     3481 gatgttgtaa caccgccact tagttgtcta taaatattta tatcttctgg gtctaaaatg
     3541 tcgcctattc ttacttcgga tgttactgca ttggtacatt cattaacacc gcctgttatt
     3601 gctatatgag aatgttcgtc tataattcct gcagttaaat gtttaccagt tccatcaatg
     3661 gttttccaac tcatatctaa tgttactggc aagtgttttc ctattctttc aatttttcct
     3721 ttattaataa ttacatctgt gttttctaat tttccatctt tttcatttgt ccacactgta
     3781 acatttctta ttaatatttt ttcttgcatt ggtgcaaatt cttttttata tccatagcca
     3841 ctaaaaggat atacaacatc gttaattgtt atggcagttt tatctgttga atgtgtagtg
     3901 tttttattgg atgtatctac ttttgccgat ttcccttcgt tttctttttt agcaaataat
     3961 ttccaaatta ccacatcgcc atttgctaaa tagccattgc ctgcccaagc atcagagcca
     4021 attattccgc ttaaattatt ttgttttcct ttatcggctt ttgcagacca attaattttt
     4081 actaactgat tattgagtgt aagggttgta ggaatattta ctgaatcttc ataaggtttt
     4141 aatgttaatg ttggtttttc agaattgcct tttatggtag cttcataa
//
LOCUS       DAOQPK010000194         4172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_13996_length_4172_cov_5.116833, whole genome shotgun sequence.
ACCESSION   DAOQPK010000194 DAOQPK010000000
VERSION     DAOQPK010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13996_length_4172_cov_5.116833"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PLU36_08720"
     CDS             <1..559
                     /locus_tag="PLU36_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HMZ46871.1"
                     /translation="YRKALRLMRLAEKFNKPIVTLIDTPGAYPGLEAEERGQGEAIAR
                     NIYEMIRLKVPVICVIIGEGASGGALGIGVGDKMLMMENTWYTVISPESCSSILWRSW
                     DKKEIAAEQLKLTATDMHGFGLVDEIVPEPDGGAHWNYDKAAEILKQHIINSLNEYKN
                     ISANERINNRIAKYEKMGFWNEVTA"
     gene            586..1473
                     /gene="dapA"
                     /locus_tag="PLU36_08725"
     CDS             586..1473
                     /gene="dapA"
                     /locus_tag="PLU36_08725"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HMZ46872.1"
                     /translation="MSLRSQLSGTGVAIITPFKKDYSVDYEALGKLIDFIIEGGVNFI
                     VTLGTTGETPVLDKKEKIDIINYTFDKVNNRVPVVVGIGGNNTDALIKDFEQYPIQKA
                     AAILSASPYYSKPSQEGIFQHYKALAEACPKPILIYNVPGRTGRNVSAATTIRLANEV
                     PNIGGIKEASDDMNQCMQIIRDRPDHFLVVSGDDALTMPQLSLGFDGVISVAAQAFPS
                     QFTAMVNAGLAYNFNLAQKNHFKLLEAYDLLFVENNPAGVKGFLYELGIIENVLRLPV
                     VPLSNSVQEKVKTFLKNFK"
     gene            1510..2193
                     /gene="cmk"
                     /locus_tag="PLU36_08730"
     CDS             1510..2193
                     /gene="cmk"
                     /locus_tag="PLU36_08730"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HMZ46873.1"
                     /translation="MKKIIIAIDGFSSCGKSTLAKQLGAKLNYIFVDSGAMYRAIALY
                     FLQNNIDINKDIQVTEALKNIQLSFAFNNEKGSSDMFLNNINVEEQIREMAVSNNVSK
                     VSTLKLVRDFAVAQQQQMGIAKGIVMDGRDVGTVVFPTAELKIFVTASIDVRVERRYK
                     ELIEKNSNISKEEVKNNLQERDYIDSTRAIGPLKQAEDAKVLDNSHISREEQLAIALQ
                     WANAIIHSI"
     gene            2212..>4172
                     /locus_tag="PLU36_08735"
     CDS             2212..>4172
                     /locus_tag="PLU36_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HMZ46874.1"
                     /translation="MNLGVLHEQYKNNSCLLQLADRLLFADAQKIFLKNAQGSSPEFI
                     ITNIFSFTQTSELNHLIILNDSEEAAYFHNTLENLTNAYDLFYFPSSFKIRKNFKLLN
                     VSHVMLRTEMLTKLSVGGNKKIIVTYPEALFEKVVLPNTLHSNIISIKTNDTINLNSL
                     MELFVMYGFVRTDFVYEPGQFALRGGILDIYSFGNEKPYRIELFGNEVDSIRIFEPEK
                     QLSERKLLQVNIIPNVETQFESNDKISLLEFLSENTIIWLKDWDIIKEKLETQEEDFG
                     LFIERIKSNTNSTEEENEAEENTKILKEINLVDIVPATTIERQVQQKHIVEFGYKPYW
                     ANFEIEFNTQSQPSFNRNFDLLIQNLKKYETEKYSIYIFADQVKQLERLEAIFKDLQT
                     EIHFTPIPISIHEGFIDHQLKIVCYTDHQIFQRYHKYKVKQAFSKNKALTLKTLRDLQ
                     PDDFVTHIDHGVGRYSGLQKIEVNGKLQEAVRIIYKDSDILYVNINSLHKISKYTGKE
                     GTVPKVNKLGSDVWNKLKEKTKTKVKEIAFDLIKLYAQRKAQKGFSHTPDNYLQTELE
                     ASFIYEDTLDQAKAVADVKKDMESENPMDRLVCGDVGFGKTEVAIRAAFKTVVDGKQA
                     AVLVPTTILAFQHFKTFKERLRDFPLTVD"
BASE COUNT         1605 a          579 c          726 g         1262 t
ORIGIN      
        1 atatagaaaa gcattgcgtt taatgcgttt agccgaaaag tttaataaac caatagttac
       61 tttaatagat acaccaggag cttatccagg gttagaagca gaagaaagag gacagggaga
      121 agccattgcc agaaatattt atgaaatgat aaggcttaaa gttcctgtaa tatgtgtaat
      181 tataggagag ggagcaagtg gaggtgcttt aggtataggc gttggagata aaatgctaat
      241 gatggaaaat acctggtaca ctgttatttc tccagaaagt tgctcctcta ttttatggcg
      301 tagttgggat aaaaaagaaa ttgctgcaga gcaattaaaa cttactgcta ccgatatgca
      361 tggctttggt ttggtagatg aaattgtacc tgaaccagat ggtggagctc attggaatta
      421 tgataaggca gcagaaattt taaaacaaca cattattaat tcacttaacg agtataaaaa
      481 tataagtgca aatgaacgta ttaataacag aattgctaag tacgaaaaaa tgggcttttg
      541 gaatgaagta acagcttaat ttaatttttt aaatcagaaa tatatatgtc tttacgttct
      601 caattatcag gcactggtgt tgccattata acccctttta aaaaagatta tagtgtagat
      661 tatgaagcac ttggtaaact tattgatttt ataatagaag gtggtgtaaa ctttatagta
      721 acacttggta caaccggcga aacacctgtt ttagataaaa aggaaaaaat agacattata
      781 aactatactt ttgataaggt aaacaataga gtgccggtag ttgtaggaat tggtggaaat
      841 aataccgatg ctttaattaa agattttgaa caatacccca ttcaaaaagc tgctgctatt
      901 ttaagtgcct ctccatatta tagtaaacca agccaagaag gtatttttca acattataaa
      961 gcattggcag aagcttgccc caaacccatt ttaatttata atgtaccagg tagaacaggt
     1021 agaaatgtaa gtgctgccac cactatacgt ttagctaatg aagtacccaa tattggaggt
     1081 attaaggaag ctagtgatga tatgaaccag tgtatgcaaa taattagaga caggcccgat
     1141 cattttttag tagttagtgg agacgatgct ttaacaatgc cacaactttc attaggattt
     1201 gatggtgtta taagtgttgc tgcacaagca tttccctcac aatttactgc aatggtaaat
     1261 gcaggcttgg cttataattt taacctagct caaaaaaatc attttaaact tttagaagca
     1321 tacgatttat tgtttgtaga aaataatcct gcaggagtaa aggggttttt atatgaacta
     1381 ggaattattg aaaatgtttt gcgtttacct gttgtgccat taagcaactc tgttcaagaa
     1441 aaagtaaaaa cttttttgaa aaattttaaa taaatttcct tccaattttt tctcaagata
     1501 ttttagttaa tgaagaaaat aattattgct attgatggat tttcatcttg cggaaagagc
     1561 actttagcaa aacaattagg tgctaaacta aactacattt ttgtagatag tggtgcaatg
     1621 tatagagcaa tagcattgta ttttttacaa aataatattg atatcaataa ggatattcaa
     1681 gtaacggaag ctttaaaaaa tatacaatta tcttttgcat tcaataatga aaaaggcagc
     1741 agtgatatgt ttttaaataa tataaatgta gaagagcaaa taagagaaat ggcagttagc
     1801 aacaatgtta gcaaagtaag tacattaaaa ctagtacgag attttgccgt agcacaacaa
     1861 caacaaatgg gcattgcaaa aggcattgta atggatggaa gagatgttgg cactgttgtt
     1921 tttcctactg ccgaattaaa aatttttgtt acagcaagta ttgatgtacg tgtagaaaga
     1981 aggtataaag aattgataga aaaaaattct aacataagta aagaagaagt aaaaaataat
     2041 ttacaagaaa gagattatat agatagcacc agagctattg gcccacttaa acaagcagag
     2101 gatgcaaaag tgttagacaa tagccatatt tccagagaag aacaattagc aattgcatta
     2161 caatgggcta atgcaataat ccatagcatt taattatatt tgcacccctg tatgaatttg
     2221 ggtgttttgc atgaacagta taaaaataat tcctgccttt tacaattggc agacaggttg
     2281 ctatttgccg atgctcaaaa aatatttcta aaaaatgcac aaggcagttc gccagagttt
     2341 attatcacta atattttttc tttcacccaa acatcagaac ttaatcattt aattatctta
     2401 aatgattctg aagaggcagc ctattttcat aataccttag aaaacttaac caacgcttac
     2461 gacctttttt attttccatc ctcctttaaa atacgaaaaa attttaaact acttaatgta
     2521 tcgcatgtaa tgcttagaac agaaatgctt actaagcttt ctgttggcgg taataaaaaa
     2581 ataattgtta cttaccccga agccttattt gaaaaagttg tactacccaa tactttacat
     2641 agcaatatta ttagcataaa aaccaatgat actattaacc taaatagttt aatggagttg
     2701 tttgtaatgt atgggtttgt aagaacagat tttgtgtacg aacctggaca atttgcatta
     2761 cgtggcggca ttttagatat ttactcattt ggcaatgaaa aaccctatag aatagagctg
     2821 tttggcaacg aagtagatag tataagaatt tttgagccag aaaagcaact tagtgaaaga
     2881 aaactattac aagtaaatat tatacctaat gtagaaacac agtttgagag taacgacaaa
     2941 atttctttac tagaattttt atcagaaaat accattattt ggcttaaaga ttgggatatt
     3001 ataaaagaaa aattagaaac ccaagaagaa gactttggat tgtttataga aagaataaaa
     3061 agcaatacta atagcacaga agaagagaat gaagcagaag agaatacaaa aattttaaaa
     3121 gaaattaatt tagtagatat tgtacctgct acaacaattg aaaggcaagt acaacaaaag
     3181 catattgttg aatttggtta taaaccatat tgggcaaatt ttgaaataga atttaataca
     3241 caatcgcaac cttcatttaa tagaaatttt gatttactta ttcaaaattt aaaaaaatac
     3301 gaaacagaaa aatattccat ttacatattt gcagatcaag taaaacaatt agaaagatta
     3361 gaggccattt ttaaagattt acaaaccgaa attcatttta caccaattcc aatttctata
     3421 catgagggtt ttatagacca ccaattaaaa attgtttgct ataccgatca tcaaattttt
     3481 caacgctatc acaaatacaa agtaaaacaa gcgtttagta aaaacaaagc tcttacatta
     3541 aaaactttaa gagacttaca acccgacgat tttgtaacac atatagatca tggtgtaggc
     3601 agatatagtg gtttgcaaaa aatagaagtt aatggaaaac tacaagaagc tgtacgaatt
     3661 atttataaag acagcgatat tttatatgta aacataaaca gccttcataa aatttctaaa
     3721 tatacaggca aagaaggcac tgtgccaaaa gttaataaat taggtagcga tgtttggaat
     3781 aagctaaaag aaaaaactaa aaccaaagta aaagaaatag catttgattt aataaaacta
     3841 tatgcacaac gtaaggcaca aaaaggattt tctcatacgc cagataatta tttacaaacc
     3901 gagttagaag caagctttat ttatgaagat actttagacc aggcaaaagc agtagccgat
     3961 gtgaaaaaag atatggaaag tgaaaatcca atggatagat tggtatgtgg cgatgttggc
     4021 tttggtaaaa cagaagtagc cattagagca gcatttaaaa ctgtggttga tggcaaacaa
     4081 gctgctgtat tagttccaac tacaatttta gcatttcaac actttaaaac ttttaaagaa
     4141 aggctcagag attttccatt aacagtagat tt
//
LOCUS       DAOQPK010000195         4156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_14078_length_4156_cov_4.079249, whole genome shotgun sequence.
ACCESSION   DAOQPK010000195 DAOQPK010000000
VERSION     DAOQPK010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14078_length_4156_cov_4.079249"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="PLU36_08740"
     CDS             complement(<1..827)
                     /locus_tag="PLU36_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HMZ46875.1"
                     /translation="MEAQVNNMMLTNDMLSYEGFKATVLNDFRVALISRETSLLGRKE
                     VLTGKAKFGIFGDGKEVAQIAMAKCFKAGDFRSGYYRDQTFMFATGLANVEHFFAQLY
                     ADPIEGNDPFSGGRQMNCHFATKFVDDKGNWLDLANRKNISSDIAPTAGQMVRGLGLA
                     FASKCFRNSSTLQNLDQLSHNGNEVCFCTIGDASTSEGNFWETINAAGVLQVPLAVFV
                     YDDGYGISVPKAFQTTKESISTVLKGFQKQNDTNGINIYRVKAWDYAGMCEVLENAI"
     gene            1064..1546
                     /locus_tag="PLU36_08745"
     CDS             1064..1546
                     /locus_tag="PLU36_08745"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HMZ46876.1"
                     /translation="MKKIALFTFIALFSIAVNAQQQKTNAPAETTKPQPKDVNKVLEF
                     KNAEYNFGKIPFGKPADYELSFKNVSNDTVTLVRVQVSCGCTSPKYKEGQKIAPGETG
                     HITLGFNGSTNGVFTKFVTVFFNDNMSKQVLFKGETYQTPDTSAPANGGVQKMKTGGK
                     "
     gene            1576..1965
                     /locus_tag="PLU36_08750"
     CDS             1576..1965
                     /locus_tag="PLU36_08750"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HMZ46877.1"
                     /translation="MKNLLLIALLFCSVNVIAQTPAQFKVVKHNFGKIKQNVPVSYVF
                     KFTNNSDKPLIIENAVAGCGCTTPEYPKQPIAKGKEGTIKVTYNAATMGVFTKDVTVK
                     FANVQQPVVLNIVGEVIEAKASTNKKS"
     gene            complement(2076..2149)
                     /locus_tag="PLU36_08755"
     tRNA            complement(2076..2149)
                     /locus_tag="PLU36_08755"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2113..2115),aa:Met,seq:cat)
     gene            complement(2229..>4156)
                     /locus_tag="PLU36_08760"
     CDS             complement(2229..>4156)
                     /locus_tag="PLU36_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HMZ46878.1"
                     /translation="TGLQLQKQDWASPYYRDDSLMLSMGFKPYELMLQLLAKKDDPFS
                     GGRSYYCHPSNKDGLRPSIIHQSSATGMQSIPTTGVAQGIKYIEEHHPTLLQKGSNNE
                     MPVVVCSFGDNSITEGEVSEAFQFAVLKELPIIYLVQDNQWGISVSAEEARTMNAFEY
                     AAGFKGLKRLQCDGSDFSRSYETMQDAFEYVRTERKPVLVHARVPLLGHHTSGVRKEF
                     YRTREDLLKHGLYDPIPKLRMLLTGIGFNEHEINEVETNAEKDANEAFEKAQLAAEPT
                     YDLTELHVFAPTNIKDEVGTRNPKSTEKTIMVDAALHAIEEIMQEFPEAVLYGQDVGR
                     RLGGVFREAATLAEKFGDHRVFNTAIQEAYIVGSTIGMSAVGLKPIVEVQFADYIYPG
                     LNQLVTEVSKSCYLSNGKFPVQMILRVPIGAYGGGGPYHSGSIESTLLTIKGIKVCYP
                     SNAADMKGLMKAAFLDPNPVVMLEHKGLYWSKVPGTEDAKTNEPSKDYILPLGKANIV
                     LQADSNKIKSGETVTIITYGMGVYWAKTAAEKFNGQIEIVDLRTLFPLDEALIFASVK
                     KHGKVLVLTEEQQNNSFAESLSLRISNNCYHFLDAKVEVMGALNLPAVPINLALEDTM
                     LPNAPKVAERIEKLLNY"
BASE COUNT         1356 a          742 c          627 g         1431 t
ORIGIN      
        1 tgaatggcgt tttccagtac ttcacacata cctgcataat cccatgcttt cactctatat
       61 atatttatac cattggtatc attttgtttt tgaaaacctt ttaatactgt actgatagat
      121 tctttggtag tttgaaaagc ttttggtacc gaaatgccat aaccatcatc ataaacaaaa
      181 acagctaaag gaacttgtag caccccagca gcattaattg tttcccaaaa attaccttca
      241 cttgtactag catctccaat ggtgcaaaag caaacttcat taccattatg agatagttgg
      301 tctaaattct gaagagtgga agaatttcta aaacatttac tggcaaaagc caaacctaag
      361 cctcttacca tttgccctgc agttggggca atatctgaag aaatattttt tctattagct
      421 aaatctagcc aattgccttt gtcatccaca aatttggttg caaaatggca attcatttgc
      481 ctaccaccac taaaaggatc attaccttct attgggtcgg catatagttg agcaaaaaaa
      541 tgctctacat tagcaagccc agtggcaaac ataaaggttt ggtctctata atatccactt
      601 ctaaaatcgc cagccttaaa gcattttgcc attgctattt gtgcaacttc tttaccatct
      661 ccaaaaatgc caaacttagc tttgccggtt aaaacttctt ttctgcctaa aagactggtc
      721 tctctactaa ttagtgctac cctaaaatcg tttaaaacag tagctttaaa gccttcataa
      781 gaaagcatat cattggttaa catcatattg tttacttgag cttccattaa gcaaaagtaa
      841 ctatttgagt ttgctttaaa aagctttata aaaataaaaa ataatactaa aaattatgtt
      901 gtttaaataa atggttgaat ttttaaataa ttttttttta atgagattac gaaaagctta
      961 ttttggcata gttttagcaa aaaactattg aactttttaa taaaaaattt aactttacca
     1021 ttccaaacgc cgttatacac ggcattaaaa ttaaaaaatg aatatgaaaa aaattgcctt
     1081 atttactttt attgctttgt tttcaattgc tgtaaatgca caacaacaga aaactaatgc
     1141 tccggcagaa actaccaagc cacaacctaa agatgtaaac aaagtgttgg agtttaaaaa
     1201 tgcggaatac aattttggga aaattccttt tggtaagcct gcagattatg aattaagttt
     1261 taaaaatgta agtaacgata cagttactct agtaagagta caagtttctt gtggttgtac
     1321 ttctcctaaa tacaaagaag gtcaaaaaat tgcacctggc gaaacagggc atataacatt
     1381 gggttttaat ggtagtacta atggagtatt tacaaaattt gttactgtat tttttaatga
     1441 taatatgagt aaacaagttt tatttaaagg agaaacatac caaacacctg atacaagtgc
     1501 acctgcaaat ggcggagtac aaaaaatgaa aactggagga aaataatttt taaaccattt
     1561 atttaatgtt ataacatgaa aaatttatta ttgattgctt tattattttg tagtgtaaat
     1621 gtaattgcac aaacaccagc acaatttaaa gtagtaaaac ataatttcgg aaagattaaa
     1681 caaaatgtac cagtatctta cgtttttaag tttaccaata acagcgataa gcctttaatt
     1741 attgaaaatg ctgtggcagg ttgtggttgt acaacacctg aatatcctaa acaaccaata
     1801 gcaaaaggta aagagggtac tataaaagta acctataatg cagcaacaat gggtgttttt
     1861 acaaaagacg taactgtaaa atttgcaaat gttcaacaac ctgtagtgtt aaatattgta
     1921 ggagaggtaa tagaagcaaa agcttcaacc aataagaaaa gctaatttgt agtttatatt
     1981 actattaaag cttcggtaat tttactgaag ctttttttat aattttttta gataaccacc
     2041 taatataaaa ccccaaactt ttgtttgggg ttttgtagtg ggaagcagac ttgaactgcc
     2101 gaccttcggg ttatgaatcc gatgctctaa ccaactgagc tatcccacca attaaatatg
     2161 cttaaaaagt atttatactt ttttgggttg caaatatagg aggtttgatt aaataagaat
     2221 aggttgtttt aataattcaa taatttttct attctttctg ctacttttgg tgcattgggt
     2281 aacattgtat cttctaatgc aaggttaata ggtactgcag gtaaatttaa agcccccata
     2341 acttctacct tagcatctaa aaagtggtaa caattattag aaattcgtaa cgataaactt
     2401 tcggcaaatg aattattttg ttgttcttct gttaatacca aaaccttacc atgttttttt
     2461 actgaagcaa aaattaaagc ctcatctaaa ggaaataatg ttcttaaatc aacaatttca
     2521 atttgaccat taaatttttc tgctgctgtt tttgcccaat atacacccat cccataagta
     2581 atgatagtaa cagtttctcc actttttatt ttatttgaat cggcttgtaa aacaatattt
     2641 gccttaccta agggtaaaat ataatctttg ctaggctcat ttgtttttgc atcttctgta
     2701 cctggtactt tgctccaata caaaccttta tgttctaaca ttactacagg gttaggatct
     2761 aagaaagcag ctttcattaa gcctttcata tctgcagcat tacttgggta acaaactttt
     2821 attcctttta tagttaataa agtgctttca atacttccac tatggtatgg tccaccacca
     2881 ccatatgcac caattggcac tcgtaaaatc atttgtacag gaaacttacc attacttaaa
     2941 tagcagcttt tagatacttc ggtaactaac tgatttaaac cagggtaaat gtaatcggca
     3001 aattgtactt ctacaattgg ctttaagcct acagcactca taccaattgt actacctaca
     3061 atatatgctt cttgaatggc tgtattaaaa actctgtggt cgccaaattt ttctgcaagg
     3121 gttgctgctt ctctaaaaac gcctcctaat cttctaccta catcttgccc atataaaaca
     3181 gcttcgggaa attcttgcat aatttcttcg atagcgtgta aagctgcatc aaccattatt
     3241 gttttttcag tagattttgg atttctagta cctacttcat ctttaatatt tgttggggca
     3301 aaaacatgta actcagttaa atcgtaggtt ggttctgcag ctaattgtgc tttttcaaaa
     3361 gcttcattag cgtctttttc tgcattggtt tctacttcat ttatttcatg ttcattaaat
     3421 ccaattccag ttaataacat tcttaatttt gggatgggat cgtataaacc atgttttaat
     3481 aaatcttctc ttgttctgta aaattctttt cttacaccac ttgtatgatg ccctaataag
     3541 ggtactcttg catgtactaa aacaggcttt ctttcggttc tcacatattc aaaggcatct
     3601 tgcatagttt cataacttct actaaaatca ctaccatcgc actgcaatct ttttaagcct
     3661 ttaaagcctg cagcatattc aaaggcattc atggttctgg cttcttctgc actaacacta
     3721 attccccact gattgtcttg cactaaataa ataattggta attcttttag tactgcaaat
     3781 tgaaatgctt cgcttacttc accttctgta atactgttat caccaaaaga gcaaacaacc
     3841 actggcattt cgttgttgct tcctttttgc agtaatgttg gatgatgttc ttcaatatat
     3901 ttaatacctt gtgctacacc tgtagttggt attgattgca tacctgttgc actactttga
     3961 tgaatgatag aaggtcgtaa accatcttta ttagatgggt ggcaataata agaacgacca
     4021 ccactaaaag ggtcatcttt ttttgccaat aattgcagca ttaattcgta aggtttaaat
     4081 cccatagata gcattaaact atcatctcta taataagggc ttgcccaatc ttgcttttgt
     4141 agttgaaggc cggtag
//
LOCUS       DAOQPK010000196         4105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_14354_length_4105_cov_4.260494, whole genome shotgun sequence.
ACCESSION   DAOQPK010000196 DAOQPK010000000
VERSION     DAOQPK010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14354_length_4105_cov_4.260494"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2009)
                     /locus_tag="PLU36_08765"
     CDS             complement(<1..2009)
                     /locus_tag="PLU36_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HMZ46879.1"
                     /translation="MEKVEMKVEGMSCTNCALTIDKYLKEQGLQNVKVNFIGGDVSFD
                     MLDNTSVEAIEKGIYKLGYTVKNDHQNSNSKQTYYGFLPFKNNLQRFWFCFVFTLPLM
                     LHMIPFLHIHFLMNPYVQLGLTIPVYVVGMDFFARSAIKSMQKGIPNMNVLITIGSTA
                     AFIYSLYGLLIGKAQDYLFFETAATTITLVFLGNYMEDKSVEQTQAALKKLAVSQKTM
                     ANMIAFDDKHEEQIFQVESKDLRVGDLVLIRNGEFVPMDCKILWGEAEVNEAIITGES
                     IPVRKATKDKLIGGSIVEAGTVKAQITAVGQDTVLANILKMIKNAETEKPPVQQMADK
                     ISAIFVPTVVSIALLTLLINYFFASQTFAESLLRSIAVLVISCPCAMGLATPAAIAVG
                     LGRGAKKGILFKNARSLEIFKSITQVVFDKTGTLTTGKFSIAQFHSTIDENDFKTLVY
                     SLEKYSNHPIGKSIAAEWKTKNDIRWSAIEEVKGLGMKATDKEGNEFIAGSFKTSNQT
                     TPQEELHNVYVSKNGKLIGWIDVKDELRPEAAQVVQILKQKGIKTILLSGDKTEKCVE
                     VQKTLGIDEIIAEQTPEQKLQHIDRLNKLSPTAMVGDGINDGPALAKATVGISLSDAS
                     QVAMQSAQVVLMNHGLKNLPEALGLGKHTYITIKENLFWAFSYNIV"
     gene            2105..2524
                     /gene="tsaE"
                     /locus_tag="PLU36_08770"
     CDS             2105..2524
                     /gene="tsaE"
                     /locus_tag="PLU36_08770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HMZ46880.1"
                     /translation="MDAIFTLQTINEVAAMFWKRYQQRKVWAFHAAMGTGKTTFIHSL
                     CNHVLNIKENISSPTFSIINEYNSPIAGTVFHTDWYRLKEEEEAINAGIEDILHSGNI
                     CLIEWPEKAINLLPDNTLHIFMEALDEHTRRIFLTAE"
     gene            complement(2552..3826)
                     /locus_tag="PLU36_08775"
     CDS             complement(2552..3826)
                     /locus_tag="PLU36_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ46881.1"
                     /translation="MTFKDTFLISFRTVRSNKLRTGITVAIIAFGIMALMGIITAIQA
                     MEHSLRESFSFMGSNAFSIRHKELTVRMGGPNNESTKHDKRKVKEKKSNLNKVITKEE
                     AEYFKNNLHYPAQVSIYRRGGGSLECSYDNKKTNPITTLFGGDENYLAVNGYTIVAGR
                     NLNSIDVNTGRSVCLIGSNTAKTLFGSNPERSIDKIIRVAGLPYRVIGLLKEKGSSGF
                     LRQDDVVITSYVNVSKLSTASSSFLIGVMVKNVSEIEVAQDEAIAAFRSVRKLHPAEA
                     DNFAIEKSDRIAEMFIGFLSGISGSAGAIGMITLIGAAIGLMNIMLVAVNERTREVGL
                     IKAIGGKSKNVRMQFLMESMIISLMGAAFGIVLGVLIGNIFAAFLKTGFIIPWNWVFA
                     GVLICTVVGLLAGLYPAQKASKLNPIDALRYE"
     gene            complement(3845..>4105)
                     /locus_tag="PLU36_08780"
     CDS             complement(3845..>4105)
                     /locus_tag="PLU36_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222996.1"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HMZ46882.1"
                     /translation="KRIVTTALKHGYTILLTADHGNADYMINEDGSPNTQHSLNLVPL
                     FLIDAHKKIHLKNGKLGDIAPTILTLMQLPIPKEMTGDVLIY"
BASE COUNT         1297 a          742 c          566 g         1500 t
ORIGIN      
        1 acaatattat aagagaaagc ccaaaataaa ttttctttaa tggtaatata ggtatgtttt
       61 cctaaaccta atgcttctgg taaatttttt aatccatgat tcattaatac tacttgtgca
      121 ctttgcatag caacctggct tgcatcgctt aaagaaatac caactgttgc ttttgctaat
      181 gcagggccat cattaatacc atctccaacc attgcagtag gagaaagttt atttaatcta
      241 tcaatatgtt gtaacttttg ttcgggtgtt tgttctgcaa ttatttcatc tatccctaag
      301 gttttttgca cctctacaca tttttctgtt ttatcgccac taagtaaaat agttttaatt
      361 cctttttgtt ttaaaatttg tactacttgt gcagcttcgg gccttaattc atcttttaca
      421 tcaatccatc caattaattt tccattttta gaaacatata cattatgaag ctcctcttga
      481 ggtgttgttt gattacttgt tttaaaagaa ccggcaataa actcatttcc ctctttatct
      541 gttgccttca tacctaaacc ttttacttct tctatggctg accagcgtat atcatttttt
      601 gttttccact cggcagctat ggatttacct attggatgat tagaatattt ttctaaggaa
      661 taaaccaatg ttttaaaatc attttcatca atggttgagt gaaattgagc tatagaaaat
      721 tttcctgtag ttaaagttcc tgttttatca aatacaactt gggttatgga tttaaaaatt
      781 tctaagcttc tggcattttt aaatagtatg cctttttttg ctcctcgtcc taagccaact
      841 gcaattgctg caggtgttgc taaacccata gcacaagggc aactaattac caacactgca
      901 atactcctta acaaactttc ggcaaatgtt tgcgatgcaa aaaagtagtt tattaataag
      961 gtaagtaatg ctatgcttac aactgttggt acaaaaatgg cactaatttt atctgccatt
     1021 tgttgtacag gtggtttttc agtttcggca tttttaatca tttttaaaat gttagctaaa
     1081 acggtatctt gtcctactgc tgtaatttgt gcttttactg ttccagcttc tacaatactg
     1141 ccaccaatta atttatcttt agtagctttt cttacaggaa tactttcgcc agttataatt
     1201 gcctcgttta cttctgcctc gccccataaa attttacaat ccattggtac aaattctcca
     1261 ttacgaatta ataccaaatc tccaactctt aaatctttgc tttctacctg aaaaatttgc
     1321 tcttcatgtt tatcatcaaa agctatcata ttagccattg ttttttggct aacagctaat
     1381 ttttttaatg ctgcttgtgt ttgttcaaca cttttatctt ccatataatt acctaaaaaa
     1441 actaatgtta tggttgtagc agctgtttca aaaaataaat aatcttgggc tttgccaata
     1501 agtaaaccat ataaactata aataaatgca gcagtactac caattgtaat tagcacattc
     1561 atatttggta ttcctttttg catgcttttt atagcacttc ttgcaaaaaa atccattcca
     1621 acaacataca caggtatggt taagccaagt tgtacataag ggttcattaa aaaatgaata
     1681 tgtaaaaatg gtatcatgtg caacattaat ggcagggtaa acacaaaaca aaaccaaaaa
     1741 cgttgcaagt tatttttaaa aggaagaaag ccatagtaag tttgttttga attggagttt
     1801 tgatgatcat tttttactgt atagcctaat ttatagatgc ctttttctat tgcttctact
     1861 gatgtattat ctagcatatc aaaactaaca tcgcctccaa taaaatttac ttttacattt
     1921 tgtaaccctt gttcttttaa atatttatca atagttaaag cacaatttgt gcaactcata
     1981 ccttctactt tcatttccac tttttccata atttttgcta caatttttta ttaaaattag
     2041 aatttgtaaa ggtacttaat atgttgtagt aactatcata ttgttaagtg aatatctttg
     2101 tatcatggat gcaatattta cccttcaaac aataaatgaa gtagcagcaa tgttttggaa
     2161 acgttatcag caaagaaaag tatgggcttt tcatgctgct atgggaactg gtaaaactac
     2221 ttttattcat agtttatgca accatgtttt aaatataaaa gagaatatta gtagccctac
     2281 attttctatc attaacgaat ataatagccc tatagctggt actgtttttc atacagattg
     2341 gtataggtta aaagaggaag aagaagccat aaatgcaggt atagaggata ttttacatag
     2401 tggaaatatt tgcctgattg aatggcctga aaaagcaata aacttattac cagataatac
     2461 tttgcatatt tttatggaag ccttagatga gcataccaga agaatttttt taactgctga
     2521 ataagttagg tgagagaaac aggttcttta attattcata tcttaaagca tctattgggt
     2581 ttagtttaga agctttttga gcaggatata aaccagccag taaaccaacc actgtacaaa
     2641 taagcacacc agcaaaaacc cagttccatg gtataataaa acctgttttt aaaaacgcag
     2701 caaatatatt ccctattaaa acccccaata caataccaaa tgctgcaccc ataaggctta
     2761 taatcatact ttccattaaa aattgcattc gtacattttt acttttacct ccaatagcct
     2821 taattaatcc tacttctctt gttcgttcat ttacagctac taacattata ttcattagcc
     2881 caatagctgc ccctattaat gtaatcattc caatagcacc tgcactaccc gaaatgccac
     2941 ttaaaaatcc aataaacatt tcggcaattc tatcactttt ttcaatggca aaattatcgg
     3001 cttcggcagg gtgcaatttt ctaacacttc taaatgctgc aattgcctcg tcttgtgcca
     3061 cttctatttc acttacattt tttaccatta cccctattaa aaaagaagaa gatgcggtgg
     3121 atagttttga tacatttaca taagaagtta ttactacatc atcttgtctt agaaaaccac
     3181 tagagccttt ttcttttaat aaaccaataa ccctataagg taagcctgcc accctaatta
     3241 ttttatcaat acttctttca ggattagagc caaataaggt tttagcagta ttactgccta
     3301 ttaagcaaac acttcttcct gtattaacat ctatactatt aagatttctg ccagcaacaa
     3361 tggtataacc atttactgcc aaataatttt catcgccacc aaacaaagtt gtaatagggt
     3421 tggttttttt attatcataa ctacattcta aactgccacc tcctcgtcta taaatactaa
     3481 cttgtgcagg gtagtgcaaa ttatttttaa aatactctgc ttcttctttg gtgattactt
     3541 tatttaagtt cgattttttt tcttttactt ttcttttatc atgtttggta ctttcattat
     3601 ttggcccacc cattctaaca gtaagttctt tatgcctaat actaaaagca ttggatccca
     3661 tgaatgaaaa actttctctt aaactatgct ccattgcctg aattgcagtt ataatgccca
     3721 ttaaggccat aataccaaaa gctataattg caacagttat acctgtacgt agtttattgc
     3781 tacgaactgt tctaaacgaa ataagaaaag tatctttaaa agtcatttaa ataaaaaaat
     3841 attattaata gattaataca tcaccagtca tctcttttgg aataggtaac tgcattaatg
     3901 ttaaaatggt tggtgcaata tcgcctaatt ttccattttt taggtgtatt tttttatgag
     3961 cgtcaattaa aaataaagga accagattta aagagtgttg tgtgttaggg ctgccatctt
     4021 cattaatcat ataatctgca ttgccatgat cggctgttag taaaatggta tagccatgtt
     4081 ttaatgctgt agtaactatt ctttt
//
LOCUS       DAOQPK010000197         4081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_14500_length_4081_cov_4.235718, whole genome shotgun sequence.
ACCESSION   DAOQPK010000197 DAOQPK010000000
VERSION     DAOQPK010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14500_length_4081_cov_4.235718"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1823
                     /gene="htpG"
                     /locus_tag="PLU36_08785"
     CDS             <1..1823
                     /gene="htpG"
                     /locus_tag="PLU36_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HMZ46883.1"
                     /translation="KKFLYSEHDIFLRELISNATDATQKLKTLSSIGEAKGELGDLRI
                     DVVLNAKEKTICFEDRGVGMTKEEVDKYLNQVAFSGAEEFLQKYKDASIIGHFGLGFY
                     SAFMVAEKVEVITQSHNADAATVYWSCDGSPEFELYEVEKKQRGTKIILHINDESKEF
                     LEAHKLKEILNKFCKYLPIPIYFTDIAEEKKEGEEEKSINNTNPIWVKKPSDLTKEDY
                     ENFYRELYPFGETPLFWIHLNVDYPFNLTGILYFPKIKQSYEIQKDKIQLYSNQVFVT
                     DEVKDIVPEFLMLLHGVIDSPDIPLNVSRSYLQGDPNVKKINNHITKKVADKLEEIFN
                     KERTEFEQKWESLGLFVKYGMMTDDKFLEKANKFLLLENTTENKFYTLEEYKTTTEAL
                     QKNKEGKQVILYTTNPIQQDAYIQAAQAKGYNVVKMETIVDAAFINNMEMKWENVTFT
                     RVDADITDNLIDKQDTTESVLSKNEEEELKKLFEIKENEITVSVEIKGLQKDSQPVIA
                     TRPEFMRRMKDMASMGGGMSAFYAQMPDEVTLTVNGNHPIYQTLLKESDIEKKEKQVR
                     NLADLALLSQGLLKGAALTNFINRSVYLMQNEETKIAATV"
     gene            2013..4049
                     /gene="uvrB"
                     /locus_tag="PLU36_08790"
     CDS             2013..4049
                     /gene="uvrB"
                     /locus_tag="PLU36_08790"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HMZ46884.1"
                     /translation="MTFKLHTSYQPSGDQPTAIQQLVDGVNNGEQFQTLLGVTGSGKT
                     FTIANVIEKIQRPTLVLTHNKTLVAQLYGEFKQFFPDNAVGYFVSYYDYYQPEAYMPV
                     SNVFIEKDLSINEELDKLRLQATSQLLSGRRDVIIVASVSCIYGMGNPTEFENGIIRI
                     KKGQTISRQGFLHNLVNVLYHRSQGDFSRGTFRVNGDTVDINLPYVDYGIRITFFGNE
                     IEEIESLEISSSKKISAMDDAAIFPANLYLAPKDMIQQVMNDIQDEMMEQVAYFKTQG
                     KLLEAQRIKERVEYDIEMIRELGYCSGIENYSRFFDRRKPGTRPFCLLDYFPKDFVLV
                     IDESHQTIPQVGGMYGGDRSRKMVLVEYGFRLPSALDNRPLNFHEFENQIGQTIFVSA
                     TPGDYELKKTEGIIVEQVVRPTGLLEPPIEVRPSVNQIDDLLEEIDKRVKKNDRVLVT
                     TLTKRMAEEMDKYLQRINIKSKYIHSDVDALERVEILRQLRLGEIDVLVGVNLLREGL
                     DLPEVSLVAILDADKEGFLRNERSLTQTAGRAARNVNGLVIFYADKITQSMQRTIDET
                     NRRREKQIAYNVMHNITPKTVSKSIQQIMQQTSVLDIKGFDETKQYAIIDGDEMFNSA
                     AEEKRTYKTIPQMEKAIASLKKKMEKAAKDLDFIEAAKLRDEMFRMEKELQSMK"
BASE COUNT         1639 a          528 c          738 g         1176 t
ORIGIN      
        1 ttaagaaatt tttgtacagc gaacacgata tttttttaag agaattaata agcaatgcaa
       61 ccgatgctac tcagaaacta aaaactttat cttccattgg agaagcaaaa ggcgaacttg
      121 gtgatttaag aatagacgtt gtattaaatg caaaagaaaa aacaatttgt tttgaagacc
      181 gtggtgttgg aatgactaaa gaggaagtag ataaatacct taatcaggtt gcctttagcg
      241 gtgcagaaga atttttacag aaatataaag atgcaagcat tattgggcat tttggtttag
      301 gtttttatag tgcatttatg gttgcagaaa aagtagaagt aattacacaa agccataatg
      361 cagatgctgc aacagtttac tggagttgtg atggcagccc agaatttgaa ttatacgaag
      421 tagaaaaaaa acaaagaggc accaaaatta ttcttcatat taacgatgaa agcaaagaat
      481 ttttagaagc tcacaaacta aaagaaattt taaataaatt ttgtaaatac ttaccaatac
      541 caatttattt taccgatata gctgaagaaa agaaagaagg tgaagaagaa aaaagtatca
      601 ataataccaa tccaatttgg gtaaaaaagc caagcgattt aactaaagaa gattatgaaa
      661 atttttatcg tgaactgtat ccatttggag aaacaccttt attttggatt catttaaatg
      721 tagattatcc ttttaacctt actggtattt tatattttcc aaaaattaag cagagctacg
      781 aaatacagaa agataaaatt caattataca gcaaccaggt ttttgtaact gatgaagtaa
      841 aagatattgt tccagaattt ttaatgttgt tgcatggtgt aatagatagc ccagatatac
      901 ctcttaacgt tagtagaagc tatttacaag gcgaccccaa tgttaaaaaa attaataacc
      961 atatcaccaa aaaagtagca gataaattag aagaaatttt taataaagaa agaactgagt
     1021 ttgagcaaaa atgggaaagt cttggcttat ttgtaaaata tggaatgatg accgatgata
     1081 aatttttaga aaaagccaat aaatttttat tattagaaaa cacaacagaa aataaatttt
     1141 atactttaga agaatacaaa acaactacag aagccttaca aaaaaataaa gaaggtaaac
     1201 aggtaatact ttatacaacc aacccaattc aacaagatgc ttatatacaa gctgcacaag
     1261 caaaaggata taatgttgta aaaatggaaa ctattgtaga tgctgccttt attaataaca
     1321 tggaaatgaa atgggagaat gtaacattta caagagttga tgcagatata acagataatt
     1381 taattgataa gcaagataca acagaaagtg ttttatctaa aaatgaagaa gaagaactaa
     1441 aaaaattatt tgaaataaaa gaaaatgaga taactgtaag tgttgaaata aaaggcttac
     1501 aaaaagattc tcaaccagta attgccacca gaccagagtt tatgcgtaga atgaaagata
     1561 tggcaagtat gggaggtgga atgtcagctt tttatgctca aatgcccgat gaagtaacct
     1621 taacagtaaa tggcaatcat cctatttatc aaaccttatt aaaagaaagt gatatagaga
     1681 aaaaagaaaa acaagttcgt aatttggcag atttagcatt actttctcaa ggcttactaa
     1741 aaggtgctgc acttacaaat tttataaaca gaagtgttta tttaatgcag aatgaagaga
     1801 ccaaaatagc agcaactgtg taatattgtt ttaaccatta tttattcaag attgttttaa
     1861 aatcttttgt agtaaaaact taaagcaacc tttatatttt aatttttcag cccaataatt
     1921 ttgttaatat tattattggg cttttttatg taaagagttg catcatagta aatttattta
     1981 tttaaactaa ttccttttaa gtattttaca ccatgacttt taaattacac acttcctatc
     2041 aaccaagtgg cgaccaacca acagcaattc agcaattggt ggatggtgta aataatggag
     2101 aacaatttca aacactattg ggtgtaactg gcagtggcaa aacttttacc attgccaatg
     2161 ttatcgaaaa aattcagcgg ccaactttag tgctaactca taacaaaaca ttagtagctc
     2221 agttatatgg cgagtttaag caattttttc cagacaatgc agtaggatat tttgtaagct
     2281 actacgatta ttatcagcca gaagcttata tgcctgtaag caatgttttt atagaaaaag
     2341 atttaagtat taatgaagag ttagacaaac ttcggttaca agcaaccagc caattattaa
     2401 gtggcagaag agatgtaatt attgttgcca gtgtaagttg catttatggt atgggcaacc
     2461 caacagagtt tgaaaatgga ataatcagaa taaaaaaagg gcaaaccatt tctcgccaag
     2521 gttttttaca taaccttgta aatgttttgt atcatcgtag ccagggagat tttagcagag
     2581 gaactttcag agtaaatgga gatacagtag atattaattt accttatgta gattatggta
     2641 taagaataac attttttggc aacgaaatag aagaaattga aagccttgaa ataagttctt
     2701 ctaaaaaaat ttcagctatg gatgatgcag ccatttttcc tgccaactta taccttgcac
     2761 ctaaagatat gattcagcaa gtgatgaatg atatacaaga tgaaatgatg gagcaagtag
     2821 cttattttaa aactcaagga aaattattag aagcacaacg tataaaagaa agagtggagt
     2881 acgatataga aatgattaga gaattaggtt actgcagtgg tattgaaaat tattcgagat
     2941 tttttgatag aagaaaacca ggaaccagac cattttgttt gttagattat tttcctaaag
     3001 attttgtatt ggtaattgat gaaagccatc aaaccatacc tcaagttgga ggaatgtatg
     3061 gaggcgatag aagtagaaaa atggtattgg tagaatatgg atttagattg ccatctgcct
     3121 tagataacag gcctttgaat tttcatgaat ttgagaacca aattggacaa accatttttg
     3181 taagtgccac acctggcgac tatgaattaa aaaaaacaga aggaattata gtagaacaag
     3241 tagtaaggcc aacaggttta cttgaacctc ctattgaagt aagaccaagt gtaaaccaaa
     3301 tagatgattt gttagaagaa atagataaac gtgttaaaaa aaatgacagg gttttagtaa
     3361 caacacttac taaaagaatg gcagaagaaa tggataaata tttacaacgc atcaacataa
     3421 aatcaaaata cattcatagc gatgtggatg ctttagaacg tgtagaaata ttgcgacagt
     3481 tgcgtttggg agaaatagat gttttagttg gagtaaattt attaagagaa ggattagatt
     3541 tacctgaagt aagtttagtt gccattttag atgcagacaa agaaggcttt ttaagaaatg
     3601 agagaagcct tacacaaaca gcaggacgtg ctgcaagaaa tgtaaacggc ttggtaattt
     3661 tttatgcaga taaaattaca caaagcatgc aacgcaccat tgatgaaacc aatagaagaa
     3721 gagaaaaaca aatagcttat aatgtaatgc ataacattac accaaaaacc gtaagtaaaa
     3781 gcattcaaca aattatgcag caaacatcag tattagatat taaaggcttt gacgaaacta
     3841 aacagtatgc aataatagat ggagatgaaa tgtttaatag tgcagcagaa gaaaaaagaa
     3901 cttataaaac tattccacaa atggaaaaag ccattgcatc tttaaaaaag aaaatggaaa
     3961 aagcagcaaa agatttagat tttatagaag cagctaagct aagagatgag atgtttagaa
     4021 tggaaaagga gttacaaagt atgaaataga aataaatgtc attccgaatg taatgaggaa
     4081 t
//
LOCUS       DAOQPK010000198         4060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_14629_length_4060_cov_3.633958, whole genome shotgun sequence.
ACCESSION   DAOQPK010000198 DAOQPK010000000
VERSION     DAOQPK010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14629_length_4060_cov_3.633958"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..1966
                     /gene="dxs"
                     /locus_tag="PLU36_08795"
     CDS             32..1966
                     /gene="dxs"
                     /locus_tag="PLU36_08795"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HMZ46885.1"
                     /translation="MEITPGKLLQNINSPDDLKKISREELHQVCSELRQYIIDVVSVH
                     GGHFGASLGVVELSVALHYIYNTPYDQLVWDVGHQAYGHKILTGRRDNFITNRKYKGL
                     SGFPKRSESEYDTFGVGHSSTSISAALGMAMASKYKGENRKSIAVIGDGSMTAGMAFE
                     AMNHAGIADADMLVILNDNCMSIDPNVGALKEYLTDIATSPTYNKVRDDIWKLLGKLP
                     IGKTFTREMASKLEHAVKGVVNKSSNLFEALNFRYFGPIDGHNITKLVDTLKDLKNIP
                     GPKLLHIVTVKGKGYALAEKDQTKWHAPGLFDKITGEIYKKQPTAPQPPKYQDVFGHT
                     MIELAEMNDKIMGVTPAMPSGSSLKFMMDKMPHRAFDVGICEQHAATLSAGLATQGMK
                     VFCNIYSSFMQRAYDQVVHDIAIQKLPVTLCLDRAGLVGDDGPTHHGCYDIPYFRCIP
                     NMIIAAPMNEQELRNMMYTSQLESTKYPFVIRYPRGEGVMPEWKTAFEQLTIGKGRKL
                     KDGENIAILTFGHPGNFAQAAIRELRTNGINPAHYDLRFVKPIDEDLLHEVFTNYNKV
                     ITVEDGTVVGGFGSAVVEFMAAHHYNAEIKILGIPDRIVEHGTLKELHRECEYDTEAI
                     VEAVKNMSNIKVKTEQMLMG"
     gene            complement(2130..2816)
                     /locus_tag="PLU36_08800"
     CDS             complement(2130..2816)
                     /locus_tag="PLU36_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ46886.1"
                     /translation="MLDVNVKKILIADDEPDIIEIIQYNLLKEGYIVYTAKNGDEAID
                     KAKKINPDLIILDIMMPKKTGIEVCTILRTQAMFKETMVVFLTALSDEETHIKGLEIG
                     ADDFISKPISSKVLVSRINALFRRISKEEVKAINIGNLTIDPLKFIVLKNGNEIILAK
                     KEFELLYLLASDIGKVFLRNEILQHVWGANVIVGDRTIDVHVRKIRQKLNLNCITTVK
                     GVGYKFVIEA"
     gene            2969..>4060
                     /locus_tag="PLU36_08805"
     CDS             2969..>4060
                     /locus_tag="PLU36_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46887.1"
                     /translation="MKLLVSILLFITTGFSLAAQSFVPTVPIPMPQVPTMRQLNHDII
                     DKSQKLITSLDGKDDGVFTVSVSDSLNQITNDALQIIVDNMQAEIELNKLIDENNKYK
                     WLRSLNDMLSSFISGYRVKLFKGNILYNLIISYKEAIELEWKNESIKPIVERNIFEIG
                     EILIKNFALKNNVGVAESEDIILLKRIERYPHRTMSLLSQNPNAYFADSVIVSQAFKD
                     PEQLYNYAAAPNALGRRIQASNNPLVKRIGLLALMKTGRMYFPFLDNLYREKISMDSI
                     TKMLHDSLGYFKLLVTTQIDYAKRLKQNDTPLVMNVLTNKLKSKAVELYVNQINALHD
                     AQENIRFECLKQLSPQDLYYVAVLGEEEIY"
BASE COUNT         1447 a          594 c          665 g         1354 t
ORIGIN      
        1 actattttta cagcacaaat aatttataaa aatggagatt actcccggaa aattattaca
       61 aaatattaat agccctgatg atttaaagaa aataagcaga gaagaattac atcaggtttg
      121 tagtgagtta agacaataca ttattgatgt agtaagtgtg catggtgggc attttggtgc
      181 aagtttagga gtggttgaat tatctgttgc actacattac atttataata caccttacga
      241 tcaattggtt tgggatgttg gccatcaggc ttatggtcat aaaatactta ctggcagaag
      301 agataatttt attaccaacc gtaaatacaa aggcttaagt ggttttccta aacgtagtga
      361 aagtgagtat gatacttttg gtgtgggcca ctcttctact tcaatttctg ctgccttagg
      421 tatggcaatg gcttcgaaat ataaaggaga aaacaggaaa agtattgctg taattggcga
      481 tggaagtatg actgctggaa tggcttttga agcaatgaac catgcaggta ttgcagatgc
      541 agatatgttg gttattttaa acgataactg tatgagcatt gaccccaatg taggggcatt
      601 aaaagaatat ttaactgata ttgcaaccag cccaacatac aataaagtta gagatgatat
      661 atggaaacta ttaggcaaat tgccaattgg taaaactttt accagagaaa tggcaagcaa
      721 actagagcat gctgtaaaag gtgtagtaaa taaaagtagt aacttatttg aagctttaaa
      781 ctttagatat tttggaccaa ttgatggcca taatattact aagttggtgg atacattaaa
      841 ggatttaaaa aacattcctg gtcctaaact attacatatt gtaactgtaa aaggcaaagg
      901 ctatgcattg gcagaaaaag atcaaactaa atggcatgct ccaggtttat ttgataaaat
      961 aactggagaa atttataaaa aacaacccac tgcaccacag cctcctaaat accaagatgt
     1021 atttggacat actatgatag agttggcaga aatgaatgat aaaataatgg gcgttacacc
     1081 tgccatgcca agtggttctt ctttaaaatt tatgatggat aaaatgcctc atagagcttt
     1141 tgatgttggc atttgcgaac aacatgcagc aactttaagt gctggcttag ctacgcaagg
     1201 catgaaagtt ttttgcaaca tttactcctc ttttatgcaa agagcttacg accaggttgt
     1261 acatgatatt gccattcaaa aattaccagt aacactttgt ttagatagag caggcttggt
     1321 aggagatgat ggaccaacac atcatggttg ttatgatatt ccttatttcc gttgtattcc
     1381 taacatgatt attgctgccc caatgaatga acaagaattg agaaatatga tgtacacttc
     1441 tcaattagaa tctacaaaat atccttttgt aattcgttat ccaagaggcg aaggtgttat
     1501 gccagaatgg aaaactgctt ttgaacagtt aaccattgga aaaggaagaa agctaaaaga
     1561 tggagagaat attgctattt taacttttgg tcatcctggc aactttgcac aagctgcaat
     1621 aagagaactt agaactaatg gtattaatcc tgcacattac gatttgcgtt ttgtaaagcc
     1681 aattgatgaa gatttgttac acgaagtttt tacaaactac aataaagtta ttactgtgga
     1741 agatggtact gttgttggtg gttttggcag tgcagttgta gaatttatgg ctgctcatca
     1801 ttataatgct gaaataaaaa tattaggcat tcctgatagg attgttgagc atggaacttt
     1861 aaaagaacta catagagagt gtgaatatga tactgaagca attgttgagg ctgtaaaaaa
     1921 tatgagtaat attaaagtaa aaactgagca aatgctcatg ggataatact ttgccaatgt
     1981 tttgcaactt tcattgcagt gtatcttaca atttagcttt gataaattac aaaacataat
     2041 ttaataattg tagcttgatt taataaatga agttataatg ttgttttaat agtgtaacaa
     2101 actctcaagg caccaattta cggttagatt tacgcttcaa ttacaaattt ataacccacc
     2161 ccctttactg tagtaataca atttaagttt aatttttgtc taatttttct tacatgcaca
     2221 tctattgttc tatcaccaac aattacgttt gcaccccata catgttgtag tatttcgttt
     2281 cttaaaaaaa ctttaccaat atctgatgct aatagatata gaagttcaaa ttctttttta
     2341 gccaatataa tttcattacc attttttaga acaataaatt ttaatgggtc tattgttaag
     2401 ttgccaatat ttattgcttt aacttcttcc ttactaattc ttctaaacaa agcatttatt
     2461 ctactaacta aaactttaga gctaattggt ttactaataa aatcatctgc tccaatttct
     2521 aaacctttaa tatgtgtttc ttcatcgctt aaagctgtta aaaaaacaac cattgtttct
     2581 ttaaacatag cttgggttct taaaatagta caaacttcaa tacctgtttt tttaggcatc
     2641 attatgtcta aaataattaa atcggggttt atttttttgg ctttatctat tgcttcatct
     2701 ccatttttag cagtataaac aatgtagcct tcttttaata aattatattg tattatttca
     2761 ataatatctg gttcatcatc tgcaattaat atttttttta catttacatc tagcataatt
     2821 tctacgcaat ttaatgctac aaaaataatc ttctaacatg tataacagca ttattaatat
     2881 tacctaattg ttatttgtca ttaacaactt atatagaaat aggcaagtat aaaatattta
     2941 cttttgtgaa acattagagc aaattagaat gaaattatta gtttccattt tactttttat
     3001 aacaacagga ttttctttag ctgcacaaag ttttgtacca actgtaccaa taccaatgcc
     3061 acaagtacca accatgcgtc agttaaatca tgatattatt gataaaagcc aaaaacttat
     3121 aacaagttta gatggaaaag atgatggagt ttttactgta agtgttagtg attctttaaa
     3181 ccaaattact aatgatgctt tgcaaattat agtagataat atgcaggccg aaattgaatt
     3241 aaataaatta attgatgaaa ataacaaata caaatggcta cgcagtttaa acgatatgct
     3301 ctcctccttt atatctggct atagagtaaa actttttaaa ggaaatattt tatacaattt
     3361 aattattagt tataaagaag caattgaact tgaatggaaa aatgaatcga taaaaccaat
     3421 tgttgaaaga aatatttttg aaattggtga aatactaatt aaaaattttg cactaaaaaa
     3481 taatgttggt gttgcagaga gtgaagatat tattttatta aaaagaatag aaagataccc
     3541 tcatagaaca atgagtttac tatcgcaaaa cccaaatgca tattttgcag atagtgttat
     3601 tgtaagccag gcttttaaag accctgagca actatacaac tatgcagcag caccaaatgc
     3661 tttaggccga agaatacaag catctaacaa ccccttggtt aaaagaattg gtttattggc
     3721 tttaatgaaa accggcagaa tgtattttcc ttttttagat aatttatatc gagaaaaaat
     3781 ttcgatggac tcaattacta aaatgttgca cgacagttta ggctacttta aacttttagt
     3841 aaccacacaa attgattatg caaaaagatt aaaacaaaat gatacaccat tggtaatgaa
     3901 tgtgcttacc aataaattaa aatctaaagc tgttgaatta tatgttaatc aaataaatgc
     3961 tttacacgat gctcaagaaa acataagatt tgaatgccta aaacaactat cgccacaaga
     4021 tttatactat gttgcagttt taggcgaaga agaaatttat
//
LOCUS       DAOQPK010000199         4039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_14767_length_4039_cov_4.284137, whole genome shotgun sequence.
ACCESSION   DAOQPK010000199 DAOQPK010000000
VERSION     DAOQPK010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14767_length_4039_cov_4.284137"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            371..1609
                     /locus_tag="PLU36_08810"
     CDS             371..1609
                     /locus_tag="PLU36_08810"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ46888.1"
                     /translation="MNKNERIILFLLACINFTHILDFMILMPLGNYLMPYFNITTTQF
                     SFLVGAYTLSAAFSGFTAAFFVDGFDRKKVLLFAYSGFLLGTLACGIAPTYHLLLAAR
                     ILAGLFGGLIGAQVLSIISDVFVYERRGKAMGAVMSAFAIASTFGVPFSLYLANLFSW
                     HAPFIFVAIVGTILIPLIQKYVPAIDHHLQNKSEKKSPFEALHIIREDRNLILALTFS
                     GLVMLGHFLIIPFINPYMEFNNGFSKQLTPMIYLVGGIASFISANILGRISDKYGKLK
                     VFSICIFISLPLVIGVTHVPAIPFWIILVMFAFWFTVATGRGVTAQAMISNAVQAKHR
                     GSFMSFNSSVQQLGTSIASLVAGVIVIKENDGKLHHYKWLGYLSVVVLLACLFLGRYI
                     FKKENYSTSQLSEDNISELI"
     gene            1701..2324
                     /locus_tag="PLU36_08815"
     CDS             1701..2324
                     /locus_tag="PLU36_08815"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HMZ46889.1"
                     /translation="MNQKKFTLLCLGDSYTIGENVPIHDSFPYQTIQLLRKKGCNFYA
                     PEIVAKTAWTSFELIEHLSGITLLENYDYVTLLIGVNNQYRGLEIEEFKKDFEWLINK
                     ALQLVGFNSNKVIVLSIPNWGVTTFAKDRDEKSITKAIESYNLICKQLAENNKTNFID
                     ITNHSLLAKDNQQLLAADGLHYSGLAMQEWAVMLANVISKDILTEKK"
     gene            2385..4025
                     /locus_tag="PLU36_08820"
     CDS             2385..4025
                     /locus_tag="PLU36_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HMZ46890.1"
                     /translation="MKFIFTCILWLFFYSIPLSAQYYLRGEIKDEKGKFLPNVKISLF
                     SKNNFRFSYTSGNSGSFGIPSTLEIDTILLTLPGYEVLKKAVNTRQYQSITMNMLAST
                     ASLMKNNLRSKTKKLLAEQYSLFSTNGESYSNLVENDFIKTSIYPETGFGLNIDRASY
                     SNIRRFLNNGVTVPVNAVRIEEMLNYFDYNLNRDKNSTKVNEFCVKNYLSTCPWDNNS
                     SLLFLTINAPKLNLDTIPPCNLVFLIDVSGSMDKPNRLPLLQSAFKMLVDNLRDIDLV
                     TIVIYGGGVAKVLDATSGSEKVAIKNVIDSLSASGDTPGQAAIQTAYASVAKTFIPGG
                     NNRVILATDGDFNVGVNSEKALEDLITGYMQSGIYLTCLGVGMGNYKDSKLETLAKKG
                     RGNFAYIDNLAEAQKVLVSEFTKTLYSVANDASINIEFNSKNISEYRLIGFDNKKSAI
                     IDNNSELEGGEVGSGHNMIAIFELKDLPNSKEKINIGEATLQFKTTDSSKKLIIQNFN
                     LANNIQAFDTISNTLKFATAVSMFGSLLKQSKFLLKSQ"
BASE COUNT         1401 a          547 c          670 g         1421 t
ORIGIN      
        1 acgctcagta gtggtggttt taccagcatc aatgtgtgct gcaataccga agttgcgttg
       61 aaattttaaa tctgccataa cgcttgtatt tgtgtgtttt aatttttttg taatgggcag
      121 ttttacaata ttcagcttcc gttgcgtcgc acacttcaac tgtataaaat atacccttca
      181 atttttttga gccgcaaagg taggggttta gacatctttt ctacaaaaag tatctttaaa
      241 atatcaaaaa aaaaattaca tatcaataaa aatagccatg ttaaaataac ctgaaattaa
      301 ttttattcat caatttacta gagaacttaa atttttaaaa cctactttgc agcccttttt
      361 gcaaattatt atgaataaaa acgaacgtat aattttattt ttactagcct gtataaattt
      421 tacccatatt ctagatttta tgatattaat gccattaggc aactacctaa tgccttattt
      481 taatattaca accactcagt ttagtttttt ggtgggagca tatacattaa gtgcagcttt
      541 ctctggtttt actgcagctt tttttgtgga tggtttcgac agaaaaaaag tattgttatt
      601 tgcttattct ggttttcttt taggcacatt agcttgtggt attgctccta cttaccattt
      661 attacttgct gcaagaattt tagctggctt atttggtggc ttaattggtg cacaagtatt
      721 atccatcatt tccgatgtgt ttgtttatga aagaagagga aaagctatgg gtgctgtaat
      781 gagtgctttt gctattgcaa gtacttttgg agttcctttt tctttatact tagccaatct
      841 ttttagttgg catgctcctt ttatttttgt agcaattgtt ggcactattc tcattccatt
      901 aatacaaaaa tatgttcctg caatagacca tcatttacaa aataagtcag aaaagaaatc
      961 gccttttgaa gcattacata ttattaggga agatagaaat ttaattttgg cacttacgtt
     1021 tagtggctta gttatgctag ggcatttttt aattattcct tttataaacc catatatgga
     1081 gtttaataac ggcttttcta agcagctaac acctatgatt tatttagttg gcggcattgc
     1141 ttcatttata tctgccaata tattaggtag aatttctgat aagtatggaa agctaaaagt
     1201 atttagtatc tgcattttta taagtttacc attggtaatt ggtgtaacac atgtacctgc
     1261 aattccattt tggattatac ttgtaatgtt tgctttttgg tttacagttg ctacaggaag
     1321 aggtgtaact gcacaagcaa tgataagtaa tgcagtacaa gctaaacata gaggtagttt
     1381 tatgagtttt aacagtagtg ttcagcaatt aggtacaagt attgcttctt tagttgcagg
     1441 ggtaattgta ataaaagaaa acgatggaaa actacaccat tataaatggt taggctattt
     1501 aagcgtagtg gttttattag cttgcctgtt tttagggaga tatattttta aaaaagaaaa
     1561 ttattcaact tctcagttat ctgaagacaa tatttcggag ttgatataaa catacaattc
     1621 accttttaaa aatcaacatt tttctacttg taatatttag ttgttattaa ttacatttat
     1681 tgctgaaaaa ggtttactaa atgaatcaaa aaaaatttac acttctttgc ttaggagatt
     1741 cttatactat tggagaaaat gtacccattc atgacagctt tccttatcaa acaatacaat
     1801 tattaagaaa gaaggggtgt aacttttatg caccagaaat agttgctaaa actgcttgga
     1861 caagctttga acttatagaa catttatctg gcataacact gctggaaaat tacgattatg
     1921 ttaccctctt gattggtgta aacaatcaat atagaggctt agagatagaa gaatttaaaa
     1981 aagattttga atggctaata aacaaagctt tacaacttgt tgggtttaac agtaataagg
     2041 taattgtatt atctataccc aattggggtg ttacaacttt tgcaaaagat agagatgaaa
     2101 aaagcataac taaggctata gaaagttata atttaatatg taaacaatta gcagaaaata
     2161 ataaaactaa ctttattgat ataactaacc attctttact tgcaaaagat aaccaacaat
     2221 tattagctgc agatggtttg cactattctg gtttagctat gcaagaatgg gcggtaatgt
     2281 tagccaatgt tatcagtaag gatattttaa cagagaaaaa ataagagcaa ttatatactt
     2341 ttttatattt aattcgttat taaaaggcta aaccttggtg tttaatgaaa tttattttta
     2401 cttgcatatt atggttattt ttttactcca ttcctttatc agctcaatat tatttacgag
     2461 gtgaaattaa agatgaaaaa ggcaagtttt tacctaacgt aaaaattagt ttattttcaa
     2521 aaaataattt tagattttct tatacaagcg gaaattctgg ttcatttgga ataccaagta
     2581 cattagaaat tgatacaata ttgctaacct taccaggtta tgaagtttta aaaaaagcag
     2641 taaacactag gcagtatcaa agtataacaa tgaatatgct agctagtaca gccagtttaa
     2701 tgaagaataa tttacgttct aaaacaaaaa aattattggc agagcaatat tctttatttt
     2761 ctactaatgg cgaaagttat agcaatttag tagaaaacga ttttataaaa acatccattt
     2821 atcccgaaac aggttttggt ttaaatatag accgtgcttc ttatagcaat atcagaaggt
     2881 ttttaaataa tggggttaca gtaccagtaa atgcagttag aattgaagaa atgctcaact
     2941 attttgatta taatttaaac cgagataaga actctactaa agttaatgaa ttttgtgtaa
     3001 aaaattattt atcaacctgc ccttgggata ataacagcag cttattattt ttaacaatta
     3061 atgccccaaa attaaattta gatactatac ctccttgtaa tttagttttt ttgatagatg
     3121 tttcaggttc tatggataaa cctaataggt taccactttt acaatcggca tttaaaatgt
     3181 tggtggataa tttaagagac atagatttag taacaattgt aatttatgga ggtggcgtag
     3241 caaaagtgct agatgcaaca agtggtagcg aaaaagtagc tattaaaaat gtaattgatt
     3301 ctttatctgc cagtggagac actccaggcc aagctgctat acaaacagct tatgcatctg
     3361 ttgcaaaaac ttttatacca ggtggcaaca acagagtaat attagcaaca gatggagatt
     3421 ttaatgttgg cgttaactcc gaaaaagctt tagaagacct aataactggt tatatgcaaa
     3481 gtggcattta cttaacttgt ttgggtgttg gtatgggtaa ttataaagat agtaaattag
     3541 aaactttagc taaaaaagga agagggaatt ttgcatacat tgataatctt gcagaagctc
     3601 aaaaagttct ggtatctgaa tttacaaaaa ctttatactc tgtagccaat gatgcttcaa
     3661 ttaatattga atttaattca aagaatattt ctgagtatag attaattggg tttgacaata
     3721 aaaaatcggc aataatagat aataatagtg aactagaagg tggtgaggtt ggaagtggtc
     3781 ataatatgat tgccattttt gaattaaaag acctacccaa ttcaaaagaa aaaataaata
     3841 ttggggaagc tacactacaa tttaaaacta cggactcaag caaaaaatta atcattcaaa
     3901 attttaactt agctaacaac atacaagctt ttgatacaat tagtaatact ttaaaatttg
     3961 ctactgctgt ttctatgttt ggtagtttat taaaacaatc aaaattttta ttgaaaagtc
     4021 aataaggggg taaaggagc
//
LOCUS       DAOQPK010000200         4029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_14824_length_4029_cov_3.645949, whole genome shotgun sequence.
ACCESSION   DAOQPK010000200 DAOQPK010000000
VERSION     DAOQPK010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14824_length_4029_cov_3.645949"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="PLU36_08825"
     CDS             complement(<1..640)
                     /locus_tag="PLU36_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HMZ46891.1"
                     /translation="MATVKKTKEKSIITEKSYQFLKEYINNASPVGFESSGQKIWLNY
                     IKPFVDTTFHDPYGTAVGVINPNDNFKVVIEAHADEISWYVNYITNEGLIYLKRNGGV
                     DHQIAPSQRVFIHGKKGAVKAVFGWPAIHTRLGSNDKEQQPKVENLFLDCGARSKKEI
                     EALGIHVGAVVTYQDGFDELANGYYVGRAFDNRIGGFMIAEVARLLKENKKKL"
     gene            complement(647..1561)
                     /locus_tag="PLU36_08830"
     CDS             complement(647..1561)
                     /locus_tag="PLU36_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46892.1"
                     /translation="MLKKLTDIFFYGNFFAGLCAVMLCIETNMQHGFALNGIHFYTII
                     FLGTAWYYTYTYVKNLPNELLDDRIIWYKKNTSSLKQTQNILLIFLIADIVFFVIKYE
                     ASFIKIASIQYGQIFLFPLVALTYTFNLLPFPEIKKLRRIGWLKPFIVGFVWSGFVSV
                     YPIIFYQIQTNIGNEIFAMPSGLLWLKNFMFISTLCVMFDLKDFEIDKKDGLKTFAVQ
                     FGVSNTIKYIIIPLVIICIAAFIIFVEQNHFPYQRILLNIIPYLFLILISFALLKKKR
                     KIIYYLFVIDGLMILKAICGILGMLIIK"
     gene            complement(1571..2053)
                     /locus_tag="PLU36_08835"
     CDS             complement(1571..2053)
                     /locus_tag="PLU36_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46893.1"
                     /translation="MKRLIFILLIFTTINSGCKKETVATKPSITIKKVGNDVVPINGS
                     MQIILSYTDKESDIDSIFIYRTRTNIKTVSGRLNDSTKLAVPSHENNRSGEIYLDFIT
                     DGVGRNDYQKYLISAIFPPVSGIPPKNESDSMLYKIIAVDKQKNKSDTAFYYPIVILR
                     "
     gene            2108..3091
                     /locus_tag="PLU36_08840"
     CDS             2108..3091
                     /locus_tag="PLU36_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HMZ46894.1"
                     /translation="MNKLCIDNILKINNEDSFKALALESFHFQFNQNLIYNKWCNLIG
                     VKPSNVSSIHQIPFLPISFFKTHSITTTNFEPTTIFESSKTTGNISSKHLIKDLAWYE
                     NSFNACFNQSYGNVEDYCILGLLPSYLERGNSSLVYMVEHFIKKSKHQLSGFYLHNYN
                     QLNIVLQQLEDEKIPTILIGVTFALLDFAEAFPMPLNHTTILETGGMKGRKKEILRTE
                     VHSILKNAFQTKNIHSEYGMTELLSQAYSTQNGIFNCPNWMKVIVRNEDDPMQIQQSG
                     KGVLNIIDLANKYSCSFIATDDAAIVNDNGSFEIQGRVDNSDIRGCSLLLL"
     gene            3203..3979
                     /locus_tag="PLU36_08845"
     CDS             3203..3979
                     /locus_tag="PLU36_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ46895.1"
                     /translation="MFKKITDINVLIVEDNEVNQIVLKRILQLNKITNFIVNNGEDAL
                     TALHNNTFDVILMDIQLPIKNGIETTIEIRQLKDTKINKIPIIALTANGLKGEEHKYQ
                     KAGMNGYLTKPFKENDLLNILHSVVINKSNSFNTKHTVNNSSTEKMYNLVQVYELVQG
                     NEEFVKNLAQIYLDTIPSTAKEMVQEIEKHNWGMASKLAHKLKSTIDTMQIASIKEDI
                     RAIEFNGKNATNTDSLLELGKNVERVINEVAVQLKEEFSL"
BASE COUNT         1494 a          562 c          550 g         1423 t
ORIGIN      
        1 gtagtttctt cttgttttct tttaataatc ttgctacttc tgcaatcata aatccaccta
       61 ttctgttatc aaatgctctg cctacataat acccatttgc caactcatca aacccatctt
      121 gatacgtaac aacagctccc acatgaatgc ctaatgcttc aatttctttt ttacttcttg
      181 caccacaatc taaaaataaa ttctctactt taggttgttg ttctttatca ttacttccaa
      241 gccttgtatg aatagcaggc caaccaaaaa ctgcttttac tgcacctttt ttaccatgaa
      301 taaatacacg ttgagatgga gcaatttgat ggtcaacacc accatttctt ttcaaataaa
      361 ttaaaccttc gttggtgata taatttacat accaacttat ttcgtcagca tgggcttcaa
      421 ttactacttt aaaattatca tttgggttta ttacacctac agcagtgcca taagggtcat
      481 gaaaagtggt atccacaaat ggtttaatat aatttaacca aattttttga ccagaacttt
      541 caaatccaac aggtgatgcg ttattaatat actcttttag aaattgatac gatttttcgg
      601 tgatgattga tttttctttt gtttttttta cagttgccat gttttattat ttaatgataa
      661 gcatacctaa aatgccacaa attgctttta aaatcattaa gccatctatc acaaaaaggt
      721 aataaattat ttttcttttc ttttttaata atgcaaatga aattaatatt agaaataagt
      781 aaggaataat gtttaataaa attctttggt aaggaaaatg attttgctct acaaaaatta
      841 taaatgcagc aatacaaata ataaccagag gtataataat atatttaata gtattactaa
      901 cgccaaattg tactgcaaat gttttaagcc catctttttt gtctatttca aaatctttca
      961 aatcaaacat tacacaaagt gtagaaataa acatgaaatt ttttaaccat aacaaaccag
     1021 aaggcattgc aaaaatttca ttaccaatat ttgtttgaat ttggtaaaaa ataatagggt
     1081 aaacagatac aaacccactc catacaaagc caactataaa tggctttaac caaccaattc
     1141 ttcttaattt tttaatctct ggaaaaggta gtaagttaaa agtataagta agtgcaacca
     1201 atgggaaaag aaaaatttgc ccatattgta tgcttgctat tttaataaat gaagcctcat
     1261 actttatcac aaaaaaaaca atatctgcaa ttaaaaatat taaaagtata ttttgtgttt
     1321 gttttaagga ggatgtattt tttttatacc aaatgattct atcgtctaaa agttcgttag
     1381 gtaaattttt tacataagtg tatgtataat accatgcagt gcctaaaaaa attattgtat
     1441 aaaaatgaat gccatttagt gcaaacccat gctgcatatt ggtttcaatg caaagcatta
     1501 cagcacaaag ccctgcaaag aaatttccat aaaaaaaaat atctgttaat tttttcagca
     1561 tgttttcaaa ttatcttaaa attacaatcg gataataaaa tgctgtatcg cttttatttt
     1621 tttgtttatc aactgcaata attttatata acatactatc tgactcattt tttggtggta
     1681 tgccagaaac aggtggaaaa atggctgata tcaggtattt ttgataatca tttcttccta
     1741 caccatctgt aataaaatct aaataaattt cgccacttct attattttca tgtgaaggaa
     1801 cggctaattt agtactatca tttaaccttc cagaaacagt tttaatattt gttcttgttc
     1861 tataaataaa aatggaatct atatcagatt ctttatcggt ataacttaaa ataatttgca
     1921 ttgaaccatt tattggtact acatcatttc ctactttttt aattgttata cttggctttg
     1981 ttgctacagt ttctttttta cagccactat ttattgtagt aaaaataagc aatatgaaaa
     2041 ttaatcgttt catttgtatt ttgtttctat tccttacaaa actaatgttt aattctttaa
     2101 atatatcatg aataaacttt gtattgataa tattttaaaa attaataatg aagacagttt
     2161 taaagccctt gctttagaaa gttttcattt tcaattcaat caaaatttaa tatataataa
     2221 gtggtgcaat ttaattgggg ttaagccaag caatgtatct tctatccatc aaattccatt
     2281 tttgccaatt tcatttttta aaactcattc cattactact accaattttg aacctactac
     2341 aatttttgaa agtagtaaaa caactggaaa tattagtagc aaacatttaa ttaaagattt
     2401 agcctggtac gagaatagtt ttaatgcttg ttttaatcaa tcttatggaa atgttgaaga
     2461 ctattgcatt ttaggcttat taccatctta tcttgaaaga ggtaattctt ctttggttta
     2521 tatggtggaa cattttatta aaaaaagtaa acaccaattg agtggttttt atttgcataa
     2581 ctacaaccag ttaaatattg ttttacagca attagaagat gaaaaaattc ccactatttt
     2641 aattggtgta acttttgcgt tattagattt tgccgaagca tttccgatgc cattaaacca
     2701 tactacaatt ttagaaacag gtggtatgaa aggtagaaaa aaagaaatat tgagaactga
     2761 agtgcatagt attttaaaaa atgcttttca aacaaaaaat atacatagtg aatacggaat
     2821 gacagaactt ttatctcaag cctattctac acaaaatgga atttttaatt gccccaattg
     2881 gatgaaagtt atagtaagaa atgaagatga ccctatgcaa attcaacaaa gtggaaaagg
     2941 agttttaaac attattgatt tagcaaacaa atactcctgc agttttattg ctaccgatga
     3001 tgctgcaatt gttaatgaca atggtagttt tgaaattcaa ggtagagtgg ataatagtga
     3061 tattagaggc tgtagcttgt tgttgttata atttttaatt taaagacata ttgcatttta
     3121 atttattgta aaaattaggt atccaatagt agataataaa aacttataat tggttatatt
     3181 tgagtaaata atattaatta ttgtgtttaa aaaaataaca gatattaatg ttttgattgt
     3241 agaagacaat gaagtgaatc aaattgtttt gaaaaggatt ttacaactta ataaaatcac
     3301 caattttata gttaataatg gagaagatgc tttaaccgct ttacacaata atacttttga
     3361 tgttatttta atggacattc aattacccat taaaaatgga atagaaacaa ccattgaaat
     3421 taggcaatta aaagatacca aaattaacaa aattcctatc attgctttaa ctgccaatgg
     3481 gttgaaagga gaggagcata agtatcaaaa agcaggtatg aatggttatt taacaaagcc
     3541 atttaaagaa aatgatttac taaatatttt acattctgtg gttattaata aaagcaattc
     3601 atttaataca aaacatacag taaataatag ttcaaccgaa aagatgtata atttagtaca
     3661 agtatatgaa cttgttcaag gaaatgaaga gtttgtaaaa aaccttgcac aaatttattt
     3721 agataccatt ccatctacag caaaagaaat ggtgcaagaa atagaaaaac acaattgggg
     3781 tatggcttct aaattggcac ataaattaaa aagtaccata gatactatgc aaatagctag
     3841 tattaaagaa gatattagag ctattgagtt taatggaaaa aatgcaacca atacagattc
     3901 attacttgaa ttaggtaaaa atgtagaaag agtaattaac gaagttgctg tacaattaaa
     3961 agaagagttt agtctgtaaa aatgacacta tcccataaag tgtgtaagta aaataattag
     4021 gggttcagc
//
LOCUS       DAOQPK010000201         4024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_14856_length_4024_cov_3.874528, whole genome shotgun sequence.
ACCESSION   DAOQPK010000201 DAOQPK010000000
VERSION     DAOQPK010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14856_length_4024_cov_3.874528"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2731
                     /locus_tag="PLU36_08850"
     CDS             <1..2731
                     /locus_tag="PLU36_08850"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMZ46896.1"
                     /translation="GTKIVSYTVTNSGGCSSTFTQAVTVNPSPIMAITGTSVCLNQTT
                     TFGNTSSIAAPDNISNWAWDFNNDGVADNTTQTPTNTYTAAGTYTVELKATSNNGCSD
                     STTIQVKVNPLPTATFTPVNACVNYNIALNNTSSIAAPDNIQTYSWNFGAGANPATGN
                     TANVTPLVYTSAGVKNITLNLMSNNSCTATITQTVDIFPAPTANFSVSAVCQGSATAY
                     TDLSTPTGSVTGWEWDFTSNNSIDNNTQNPSNTFASSGTFTTSLIATSSNGCKDTVSM
                     PVNVWGHTIPDFTPDKVCFGTASQFTNLTNETTNPNTGTGATYAWNFADGGTSTATNP
                     VHTYTLGGNANANYNVTLVVTSFHGCVDSVSKTVSVYAIPTASFTADSVCLGTASQLT
                     DASNGNGNTINTYQWDFLSDGSVDATGTSNPNHTFPAYGANAVSYTVSTSPATGLVCA
                     NSISTITVWVNPNPTPDFTFVNNCINAQPNTFNASSSAIAIGTNTNYAWNYGDGGSSA
                     TNAGSTSSHTYAAAGVYNVTLTVTSNKGCVKNIVKAVEVFEKPKTTIANSAPCDQVAM
                     TFTAVTQPTSGTINNWYWDFNNSVTSVEGNGQNTNFTFSGPGAQTIHLISETDKGCRD
                     TLTKVVYVNYVPQPQFTLDIPAGCPIHCVNFTDLTPAVTGPAQINQWTWNFGEGGPVT
                     NGTNASVSHCYNNSSSSQLAYYNVQLTVTTDSGCTNTIVKNNYVTVYPVPVAAYVANP
                     NPGSVTEPLIYFTNQSQNYTKWWWSFGDGPFKTDSVNVDPTHYYGSEVAQTYYSNLIV
                     QNQYGCLDTAYLPIEIKPDFAFYIPNAFTPDNNDGINDYFNGKGYGIATYEMWIFDRW
                     GEKVFYTDDLAKGWDGSVKGNSRTKQEVYVWKVKLTDVLGKKHEYIGHVTLVK"
     gene            3215..>4024
                     /locus_tag="PLU36_08855"
     CDS             3215..>4024
                     /locus_tag="PLU36_08855"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HMZ46897.1"
                     /translation="MDRLKDKFREKAVPLAAEVKDIIKNHGDIKLGEITVAQVYQGMK
                     GMTGMITETSKLDAEEGIRFRGYSIPELRKQLPKAPGGTEPLPEGIFYLMLVGELPTS
                     EDVNFITNEWQKRSNVPKHVFDVIEKLPVDTHPMTQFVLAIMAMQTESVFAKAYAKGI
                     NKKDYWDYVYEDSMNLIARLPRVAAYIYRRKYKNGVHIEPNHKLDWAANFAHMLGYEE
                     FDMKRLMRLYLTIHADHEGGNVSAHTTHLVGSALSDPYLAFAAGMNGLAGPL"
BASE COUNT         1273 a          728 c          751 g         1272 t
ORIGIN      
        1 gggaactaaa attgtttctt atactgttac aaactctggt ggctgtagta gcacctttac
       61 tcaagctgtt acagtcaatc catcgcctat aatggctatt actggaacca gtgtctgttt
      121 aaatcaaact actacttttg gtaatacttc gagtattgca gcacctgata atatctctaa
      181 ctgggcgtgg gattttaata acgatggcgt tgcagataac accacgcaaa ctcctactaa
      241 cacctacact gctgcgggta cttatacagt tgagttaaaa gctacgtcta ataatggttg
      301 tagcgatagt acaactattc aagttaaagt aaatccttta cctacagcca cctttacacc
      361 tgtaaatgcc tgtgtaaatt ataacattgc tttaaataat acctctagta ttgccgcacc
      421 tgataacatt caaacatata gctggaactt tggagctggt gctaatcctg ctactggaaa
      481 tactgccaat gtaactcctt tagtttatac cagtgcaggc gtaaaaaata ttacgctcaa
      541 cttaatgtca aataattctt gtacagctac cattactcaa acggtggaca tcttccctgc
      601 tcctactgct aattttagcg tgtcagcggt ttgtcaaggg tctgccactg cttataccga
      661 tttatcaaca cctactggct cagttacggg ttgggaatgg gattttacaa gcaataattc
      721 aattgataat aacacccaaa acccaagcaa cacctttgca agttctggta cttttacaac
      781 aagtttaatt gctacaagca gtaatggctg taaagacact gtatctatgc ctgtaaatgt
      841 gtggggacat accatccctg atttcacacc tgataaagtt tgttttggta ctgctagtca
      901 gtttactaat ttaactaatg aaactaccaa tcctaataca ggtactggcg ctacttatgc
      961 ttggaacttt gccgatggtg gaacttctac tgccacaaac ccagttcaca cctatacatt
     1021 aggcggtaat gctaatgcca attacaatgt tactttagta gttacttctt tccatggttg
     1081 tgtggatagt gttagtaaaa cagtaagtgt atatgccatt cctactgctt cctttacagc
     1141 tgattcagtt tgtttaggta ctgcctctca attaaccgat gctagtaatg gaaatggcaa
     1201 caccattaac acctatcaat gggatttctt aagtgatggc tcggtagatg caactggtac
     1261 ttcaaatcct aatcacactt tccctgctta tggggctaat gctgtaagct atactgtttc
     1321 tacttcgcca gctactggct tagtatgtgc taatagtata agtaccatta ccgtgtgggt
     1381 aaatcctaat cctacgcctg attttacatt tgtaaataat tgtatcaatg ctcaacctaa
     1441 tacctttaat gcctcttctt cggctattgc tattggtaca aatactaact acgcttggaa
     1501 ttatggcgat ggtggtagtt ctgctaccaa tgcgggttct actagttctc atacttatgc
     1561 cgcagcgggt gtgtataatg taacacttac tgttacttct aataaaggtt gtgtgaagaa
     1621 cattgtaaaa gcagtagagg tatttgaaaa acctaaaact actattgcca actctgctcc
     1681 ttgcgaccaa gtagcaatga cctttacggc ggtaactcag cctacaagtg gtactattaa
     1741 taactggtat tgggatttta ataatagcgt tacaagtgtg gaaggtaatg gtcaaaatac
     1801 aaactttaca ttctctggtc ctggagcgca aactattcac ctcattagtg aaacagacaa
     1861 aggttgtaga gatactttaa caaaagtagt gtatgtaaat tacgtacctc aacctcagtt
     1921 taccttagat attcctgcgg gttgtcctat tcattgtgta aactttactg acttaactcc
     1981 tgcagttact ggtcctgctc aaattaatca atggacttgg aattttggtg aaggtggtcc
     2041 tgttaccaat ggaactaatg cttcggtaag tcattgttat aataattcta gcagtagtca
     2101 attggcttat tataatgtac aattaacggt tacaacggat agcggttgta caaacaccat
     2161 tgtaaaaaat aattatgtaa ccgtgtatcc tgttccggtg gcggcttatg tagctaatcc
     2221 aaatcctggt tcagtaacgg agccgcttat ctacttcact aatcaatctc aaaattacac
     2281 taaatggtgg tggagttttg gtgatggacc ttttaaaaca gattcggtaa atgtagatcc
     2341 tactcattat tatggtagtg aagtggcaca aacttattac tctaacctta tcgtacaaaa
     2401 tcaatatggt tgtttagata cggcttatct ccctatcgaa attaaacctg attttgcctt
     2461 ctatattcct aatgcattta cccctgataa caatgatggt atcaatgatt actttaatgg
     2521 taaaggttat ggtattgcta cctacgaaat gtggatcttt gacagatggg gtgaaaaagt
     2581 attttatacc gatgatttag ctaaaggttg ggatggttcg gtgaaaggaa actctagaac
     2641 caaacaagaa gtgtatgtgt ggaaagtaaa actaaccgat gtgctcggaa aaaaacatga
     2701 atacatcggc catgttactc tggtgaagta aatcaatcct aaatactata atttcaaaaa
     2761 ggctacccga aaaggtagcc tttgttgtta ttttcaacat aactttaatt tatatagata
     2821 aattcaaaag ccatcggttt acagaaattt aacccagatt gtgcattaga aattagtagt
     2881 gagagttaaa agcccaataa aatatgaact tttacgaatg aagcgtagcc actgctacgg
     2941 tgaaagagaa aaagttgcga agcttcatat tttgcaggga ttttagctcg taataaaatt
     3001 gctaatgcat aatctgggtt taaccccata ttttataaaa tcttgacaat ttatgtaaat
     3061 gatatatgtc atactaatta gcctaaataa gggcaaatac agtatcttag gagtagttta
     3121 gggtaaattt aagatacttt aaaaaaatat ttataatttt aattggttta aatcatttca
     3181 ctacctaaat ttacgtgatt aaaatgaatt atatatggac aggttaaaag ataaatttcg
     3241 tgagaaagct gtgcctttgg cagcagaagt taaagatatt attaaaaatc atggcgatat
     3301 aaagcttggt gaaattaccg ttgctcaagt atatcaaggg atgaaaggaa tgacgggaat
     3361 gattactgaa acttcaaaat tagatgcaga agaaggaatt agatttagag gttattcaat
     3421 cccagaatta agaaaacaac ttcctaaagc tccaggcggc actgagcctc ttccagaagg
     3481 aattttttac ttaatgttag ttggcgaatt acctacttct gaagatgtta acttcataac
     3541 caacgaatgg caaaaaagaa gtaatgttcc aaagcatgtg ttcgacgtta ttgaaaaatt
     3601 acctgtggac acacatccga tgactcaatt tgtattagct attatggcaa tgcaaaccga
     3661 aagcgttttt gctaaagctt atgcaaaagg aattaataaa aaagattatt gggattatgt
     3721 gtatgaagat tcaatgaatt taattgcacg tttacctcgt gttgccgctt atatttatcg
     3781 tcgtaaatat aaaaatggtg tacatattga accaaatcac aaacttgact gggctgcaaa
     3841 cttcgctcac atgttaggtt acgaggagtt tgacatgaaa cgtttaatgc gtttatattt
     3901 aaccattcat gctgaccatg aaggcggaaa cgtatcggca cacactacac atttagttgg
     3961 ttctgcttta agcgatccat atttagcttt tgcagcagga atgaatggtt tagctggtcc
     4021 atta
//
LOCUS       DAOQPK010000202         4022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_14867_length_4022_cov_4.351399, whole genome shotgun sequence.
ACCESSION   DAOQPK010000202 DAOQPK010000000
VERSION     DAOQPK010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14867_length_4022_cov_4.351399"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3339)
                     /locus_tag="PLU36_08860"
     CDS             complement(<1..3339)
                     /locus_tag="PLU36_08860"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMZ46898.1"
                     /translation="MQFFVKLIYIKKFILLHFLVLLSSLIFAQKPTANFTTNVIKGCA
                     PLIVQFNDLSTNIPTNWSWDLGNGVTSTQQNPVTTYFTPGVYTIKLIAINASGADTVI
                     KTNFITVHDVPTVNFNADKQTGCFPLKVQYGDMSVANSGSITTWNWDFGDGTTSTIKN
                     PQHTYVVAGNFPVTLKIINSEGCTKTLTKPNYINVPGGVIANFDIGSISSCTLPVDVS
                     FNNTSTGTGILNYQWDFGNGNTSTLPNPTTNYNSNGSYSVTLITSNNQGCKDTLVKTN
                     GVIIGTVKADFSFVGTCLDYPTVFTNTSTPATGTYFWSFDDGTTSTTKNPVKTFAITN
                     SFNVKMVADFGACKDSVTKNITINPSPNADFTNNPTGGCLPPLSINFVPATSTNAAYL
                     WNFGDGNTSIQVSPTNIFQNLGAFNVSLITTNAFGCKDTVVKNNAVIIAPPDIKVFTE
                     GAPFSGCAPYTSNFKVYVESQDSIATYEWDFGDGTPIVTGSNPSHTYNAVGLYTIQVV
                     VTTINGCKDTLIATQTVKLYDKPTANFSATPLNACAKDNIAFTNLSSSNVSTWSWSFG
                     DGGTSTASNPIYNYTDTGFFAVRLIVSNNNCKDTLQLNNYIYINPPIALFYTVFSCDT
                     PLTRTFIDQSKGAGLTYLWDFGDGNTSTAQSPTHTYATYGIYNVKLTVTNGVCTHSAT
                     APANLSFNNPILVTTDTAFCKYALATYKINNIVPSLITSYVWDFGDGTIMSGSNIDSV
                     THAYTTNGNVAPTVIINDVAGCADTLTISPPIKIFGPNANFVNQPGTCLYDTINFTNT
                     STTDGIHNLTQVIWNYGDGITDTLTSPPYQHQYNATGFFTVKLKVLDDFGCYDTLIKP
                     NAVQITKPIAGFFSNDTIRCANSNVTFQDTSKGVNLTYLWNFGDTQTATQQNPQHPYL
                     LQGLYSVKLKVTDIFGCVDTLLKPLYIRVANSVANFKFLQGDTLGLCYPFLIETANLS
                     SDAVSVNWSFGDNGFSNLDTPSHIYTYAGKYTLKLKAYGYGGCVDSTQKDIIVRGPTG
                     TFSYSPLQFCSPTVVNFVAHTKNNATFVWDFADGVITLTPDSTISHTYNTPGIYRPGI
                     IMIDSAGCQVPIIGTDTVRVADVST"
     gene            complement(3448..3897)
                     /locus_tag="PLU36_08865"
     CDS             complement(3448..3897)
                     /locus_tag="PLU36_08865"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ46899.1"
                     /translation="MKIRNGFVSNSSSSSFIVLFPKEPKNATDVKNMLFKEDKVAREE
                     VKVTVVCNGGNIFVPNTFSPNGDGMNDVFYPRGKGVYTIKSFRIFNRWGEMVFEKTNF
                     QANDASAGWDGMYKGVKQPTDAYVYSIEIMCDNSISVPAKGSITILR"
BASE COUNT         1377 a          650 c          641 g         1354 t
ORIGIN      
        1 agtgcttaca tctgctactc ttactgtatc tgttccaata attggcactt gacatcctgc
       61 agaatctatc ataataattc ctggcctgta aataccaggt gtattgtatg tatgagaaat
      121 tgtagaatct ggtgttaatg taattacccc atctgcaaag tcccaaacaa aggttgcgtt
      181 gttttttgta tgagcaacaa agtttacaac agttggtgaa caaaactgta atggactgta
      241 agaaaatgta cctgttggcc ctcgtacaat tatatctttt tgggttgaat caacacaacc
      301 cccatagcca taagctttta gttttaaagt gtatttacct gcataagtat aaatatgaga
      361 aggtgtatct aaattagaaa agccattatc accaaaactc caatttacac taaccgcatc
      421 agaagataaa ttagcagttt ctattaaaaa aggataacac aaacccaaag tatctccttg
      481 taaaaattta aagttagcaa ccgaattggc aaccctaata tacaatggtt ttaaaagtgt
      541 atctacacat ccaaaaatat cagtaacttt taatttaacc gagtaaagcc cttgaagcaa
      601 atagggatgt tgagggtttt gttgtgttgc agtttgagta tctccaaaat tccacaaata
      661 agtaaggtta actcctttag aagtgtcttg aaatgttaca ttgctatttg cacatctaat
      721 agtatcgtta ctaaaaaaac ctgcaatagg cttggttatt tgaacagcat ttggttttat
      781 taaagtatca taacagccaa agtcatccaa cacttttaat ttaacagtaa aaaaaccagt
      841 tgcattatat tgatgctggt atggtggaga agttagtgta tctgttatac catcaccata
      901 attccaaatt acttgtgtta agttatgaat accatctgtt gtagatgtat tggtgaaatt
      961 aattgtatca tataagcaag tacctggctg atttacaaaa ttagcatttg gtccaaagat
     1021 tttaattggt ggcgaaatag ttaatgtatc tgcacaacca gcaacatcat ttataattac
     1081 agttggagct acattgccat ttgtggtgta tgcatgtgta actgaatcta tattggagcc
     1141 actcattatt gtaccatcgc caaaatccca cacataggaa gtaattaagg atggaactat
     1201 gttatttatt ttatatgtag ctaaggcata tttacaaaat gcagtatcag tagtaaccaa
     1261 aattggatta ttaaatgaaa gattagcagg tgcagttgca gaatgtgtac atacaccatt
     1321 tgttacagta agcttaacat tataaatacc ataagttgca taagtatgtg ttgggctttg
     1381 tgcagttgat gtgttcccat ctccaaaatc ccataagtaa gttaatccag ctcctttaga
     1441 ttgatctata aaggttcttg ttaaaggagt atcgcagcta aaaacagtat aaaacaaagc
     1501 aataggtgga ttaatgtata tataattatt tagttgcaat gtatctttac aattattatt
     1561 tgacactatt aaccttacag caaaaaatcc agtatcggta taattataaa tagggtttga
     1621 tgcagtggaa gttccaccat caccaaaact ccacgaccag gtacttacat tacttgaaga
     1681 taaattggtg aaagcaatat tatctttagc acaagcattt aaaggtgttg cagaaaaatt
     1741 tgcagttggt ttatcataaa gctttaccgt ttgtgttgca attaaagtgt ccttacatcc
     1801 atttatggtt gtaacaacaa cttgaatagt atataatccc actgcattat aggtatgaga
     1861 aggattacta ccagtaacta tgggtgttcc atctccaaag tcccattcat aagttgcaat
     1921 tgaatcttgc gattccacat atactttaaa gttacttgta tagggtgcac atccactaaa
     1981 tggtgcacct tctgtaaata cttttatatc tggtggtgca ataattactg cattattttt
     2041 tacaacggta tccttacagc caaaagcatt ggttgtaatt aatgaaacat taaaggctcc
     2101 taagttttga aatatattgg ttggagaaac ttggatggaa gtatttccat caccaaagtt
     2161 ccataaataa gctgcattag tagatgtagc aggaacaaaa tttatagata agggtggcag
     2221 acaaccacca gttggattgt ttgtaaagtc tgcatttgga gaagggttaa tagtaatatt
     2281 ttttgttaca gaatctttac atgcaccaaa atctgcaacc attttaacat taaagctatt
     2341 ggtaatagca aatgttttaa ctgggttttt ggttgttgag gttgtgccat catcaaaact
     2401 ccaaaaatat gtaccagttg ctggtgtgga tgtgttggtg aaaacagttg gataatctaa
     2461 gcaagttcca acaaaactaa aatctgcttt aacagttcca attattacac catttgtttt
     2521 aactaaagtg tctttacaac cttggttatt actagtaatt aaagtaacag aataggaacc
     2581 attgctatta tagttagttg taggatttgg aagtgttgaa gtgttaccat taccaaaatc
     2641 ccactgataa tttaaaattc ctgttcctgt agatgtatta ttaaatgaaa catctactgg
     2701 taaagtacaa ctagagatag agccaatatc aaaatttgct ataaccccac caggtacatt
     2761 aatatagttt ggtttggtta atgtttttgt acatccttcg ctattaatta tttttaatgt
     2821 tacaggaaaa tttcctgcaa caacataagt atgttgtggg ttctttattg ttgaagtagt
     2881 gccatcgcca aaatcccaat tccaagttgt aatacttcct gagttggcaa cactcatatc
     2941 gccatattga acctttaatg gaaaacagcc agtttgttta tctgcattaa aatttacagt
     3001 tggcacatcg tgtacagtta taaaatttgt tttaataact gtatcggcac cacttgcatt
     3061 aatggcaatt aacttaatag tatatacacc aggagtaaag taagtagtaa ctggattttg
     3121 ttgcgttgag gtaacgccat ttcctaaatc ccatgaccaa ttggttggaa tattagttga
     3181 taaatcgtta aactgaacta ttaatggtgc acaaccttta ataacattag tagtaaaatt
     3241 ggctgtaggt ttttgagcaa atattaaact acttaagagg actaagaagt gtagtaaaat
     3301 aaatttttta atatatataa gtttaacaaa aaactgcaca gataattaca tttaactttt
     3361 ataaacatca ataataatga gcaaataaaa tgctcatttg ctgctttaaa ttgttattaa
     3421 atgtaaatat agtaaaaaaa atatttttta tcttaatatg gttatactgc cttttgctgg
     3481 aacagagata ctgttatcgc acattatttc tatgctgtat acataggcat cagttggttg
     3541 tttaacacct ttatacattc catcccatcc tgcacttgca tcattagcct gaaagtttgt
     3601 tttttcaaaa accatttcac cccatctatt aaaaattctg aaacttttaa ttgtatatac
     3661 gccttttcct ctaggataaa aaacatcgtt cataccatcg ccatttggag aaaaagtatt
     3721 tggcacaaaa atatttccac cattacatac aactgtaact tttacttctt ctcttgctac
     3781 tttatcttct ttaaaaagca tattcttaac atcagtagca ttcttaggct cttttggaaa
     3841 aagaacaatg aaactacttg atgatgaatt acttacaaaa ccatttctaa tcttcattat
     3901 atttatatct aaaatacatt attaatccta acacagcagt aaaaccaaat aaataataaa
     3961 aaccaacagg ttctacatta ttgtttattg catacacttt aagtccaaat attataaaaa
     4021 gt
//
LOCUS       DAOQPK010000203         3969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_15232_length_3969_cov_5.157639, whole genome shotgun sequence.
ACCESSION   DAOQPK010000203 DAOQPK010000000
VERSION     DAOQPK010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15232_length_3969_cov_5.157639"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PLU36_08870"
     CDS             <1..482
                     /locus_tag="PLU36_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46900.1"
                     /translation="ISNIEFETLFVDDEKVDPYRKTSEYAGLLEFKIFTKNGTQKKLI
                     KNGGIIFNKTANQNAISYVPMEIGKKYPALGNPTFNFSISQTDFEKGYISIAGSLSDM
                     DPTKAIESSTIGLMTTLQELNNSTNKDLKDCVYQKNYIDIKKGDYTFRVHFIIKPVG"
     gene            complement(763..2214)
                     /gene="asnS"
                     /locus_tag="PLU36_08875"
     CDS             complement(763..2214)
                     /gene="asnS"
                     /locus_tag="PLU36_08875"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HMZ46901.1"
                     /translation="MFNKRIKVKELLQIDAKGQEVTVMGWVRTFRNNQFIALNDGSTN
                     QNIQIVAELGLLNEDSLKRITTGASLKICGKVVPSLGKGQIVEVKAITIEVLGDCDAE
                     KYPLQPKKHSLEFLREIAHLRFRTNTFSAVFRVRHALAFAIHQFFNERGFVYLHTPII
                     TASDAEGAGEMFRVTTLPIDGSAKRNDEGTIDFTEDFFGKSTNLTVSGQLEAELGAMA
                     FSEVYTFGPTFRAENSNTTRHLAEFWMIEPEVAFNDIEDNMDLAENFIQYIIKYVMNN
                     NKEDLEFLDARLAEEEKQKPQNERQEFGLLEKLNFVTENKFERITYTEAIDILINSPA
                     YKKKKFKYDVSWGIDMQSEHERYLVEKHFKKPVIVTGYPAAIKAFYMRLNEGCEPGKE
                     TVAAMDILAPGIGEIVGGSQREERYDVLLAKMKQFDIPAQEMNWYLDTRRFGTVPHAG
                     FGLGFERMVQFVTGMTNIRDVIPFARTPKNCEF"
     gene            2591..>3969
                     /gene="rho"
                     /locus_tag="PLU36_08880"
     CDS             2591..>3969
                     /gene="rho"
                     /locus_tag="PLU36_08880"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HMZ46902.1"
                     /translation="MYDILQLNDMLLPELLDIAEELKISNAKKLDKQDLIYKILDSQA
                     VKASETKEAEPKKQRIRKAVKTATANITEEAEIVNEPPRVVERKHIKPKKQKPDTNEN
                     VVPTDNVATTQQVADTVIENLAEKMLMATEDNTNTPEDNTKSTPPAKQHFHKKEPAFN
                     IEFDGVITSEGVLEMMPDGYGFLRSSDYNYLSSPDDVYVSPSQIKLFGLKTGDTVTGY
                     VRPPKEGEKYFALLKVDAINNKRPDEVRDRVPFDYLTPLFPFEKLNLFTTPQSYSTRV
                     MDLFTPIGKGQRGLIVAQPKVGKTMLLKEVANAIAANHPECYLMVVLIDERPEEVTDM
                     ERSVKAEVIASTFDEPAEKHVKVSSIALQKAKRLVECGHDVVILLDSITRLARAHNTV
                     APSSGKVLSGGVEANAMQKPKQFFGAARKIENGGSLTILATALIETGSKMDEVIFEEF
                     KGTGNMELQ"
BASE COUNT         1386 a          686 c          602 g         1295 t
ORIGIN      
        1 ctatttccaa cattgaattt gaaactttgt ttgtagatga tgaaaaagtt gacccctatc
       61 gtaaaacatc tgaatatgca ggattacttg aatttaaaat atttacaaaa aacggcactc
      121 agaaaaaatt aatcaaaaat ggcggtatta tttttaataa aactgccaac caaaacgcaa
      181 taagttatgt accaatggaa atagggaaaa agtatcctgc actaggtaat cctactttta
      241 actttagcat ttctcagaca gattttgaaa aagggtatat cagtatagca ggttctttat
      301 cagatatgga cccaacaaaa gccatagaat catcaactat aggtttgatg accactttac
      361 aggaattaaa caacagcact aataaagatt tgaaagattg tgtatatcaa aaaaattata
      421 tagatattaa aaaaggggat tacacattca gagttcattt tattataaaa cctgtcggat
      481 aattaatttt ttctattcaa ataaataaag gctgcacaag tgcagccttt attttatatt
      541 catcatttca attgtctttg ttcattgtac ttttaattgc agcttattat tcaaatgtaa
      601 ggctgctttt agctgttgct gaaaagctac ttgttcttca aatgttttat ctgtatccag
      661 caatttataa aactattgca ctgctttatt gttcgaattg tttttaagat attgcttttg
      721 ataatacttg catacaaata attattaagt aaatttcagt tattagaact cacaattttt
      781 tggtgttctt gcaaaaggta ttacatctct aatatttgtc atgcctgtta caaattgcac
      841 catacgttca aagcctaaac caaaacctgc atgaggtaca gttccaaatc ttctggtatc
      901 taaataccag ttcatttctt gtgcaggaat atcaaattgt ttcatttttg ctagtaacac
      961 atcatatctt tcttctcttt gactgccacc tacaatttca cctataccag gtgccaaaat
     1021 atccattgct gcaacggttt ctttacctgg ctcgcaacct tcatttaatc tcatgtaaaa
     1081 tgctttaata gctgcagggt agccagttac tattacaggc tttttaaaat gtttttcaac
     1141 taaatatctt tcatgttcac tttgcatatc aatgccccaa ctaacatcat acttaaattt
     1201 ctttttctta taagctgggc tatttattaa aatatcaata gcttcggtat aggtaattct
     1261 ttcaaattta ttttcagtta caaaatttaa tttttctaaa aggccaaact cttgcctttc
     1321 attttgtggc ttttgttttt cttcttctgc taatctggca tctaaaaatt caaggtcttc
     1381 tttattatta ttcattacat atttaataat gtactgaata aaattctctg ccaaatccat
     1441 attatcttca atatcattaa aggcaacttc tggttcaatc atccaaaact cggctaaatg
     1501 ccttgtagta ttgctgtttt cagccctaaa ggttggccca aaagtataaa cctcactaaa
     1561 agccattgcc cccaattcag cttctaactg tccactaaca gttaggttag tgcttttgcc
     1621 aaaaaagtct tcagtaaaat caatagtacc ttcgtcattt ctttttgcac taccatctat
     1681 aggtaatgta gttactctaa acatttcgcc tgctccttca gcatcgcttg ctgtaataat
     1741 aggagtatgt aaatacacaa acccacgttc attaaagaac tgatggatag caaaagctag
     1801 agcatgcctc actctaaaaa ctgcactaaa agtattagtt ctaaaacgca aatgtgcaat
     1861 ttctcttaaa aattctaagc tatgtttttt gggttgcaaa gggtattttt cggcatcgca
     1921 atctcctaaa acttctattg ttattgcttt aacttcaact atttgcccct ttcctaaact
     1981 tggtacaact ttgccgcaaa tttttaaaga agctccagta gttatacgtt ttaatgaatc
     2041 ctcattcagc aaaccaagtt cggccacaat ttgaatattt tgatttgtgc taccatcatt
     2101 taatgcaata aactggttgt ttctaaaggt tctcacccaa cccataacag taacttcttg
     2161 tcctttggcg tctatttgta ataattcttt tactttaatt cgtttgttaa acataatttt
     2221 attttttggg tatcaaaata tacttcacta ttcaacagtt gttgcgtcgc actcttgtac
     2281 tttttttcaa ttggcaacaa gtattcaacc aacggcaaat ataagcttga gaggtgttta
     2341 ataaataatt gacagaagta gagtaaaaaa aaaatttttt taaaaaacaa ctctgtctta
     2401 acattgcatc agaaacgagc tgaaagaatc taaagaaaac atctccagaa aaatatcagt
     2461 ttactttctt atccaaacaa aataagcagc acaaaccttt tttattattt taattatttt
     2521 atactaaccg ttgaaattaa ttcatttttc aaaaaataat cctgtaaact ttattactca
     2581 ataacaaatt atgtacgata ttttgcaatt aaacgacatg ctattaccag aattattaga
     2641 tatagcagaa gaactaaaaa ttagcaatgc caaaaaatta gacaaacaag atcttattta
     2701 taaaatttta gatagccagg cagtaaaggc cagcgaaaca aaagaggcag aacctaaaaa
     2761 acaaagaatt cgtaaagcag tgaaaactgc cacagctaac attacagaag aagcagaaat
     2821 tgtaaatgaa cctccaagag ttgtagaaag aaagcatata aaacctaaaa aacaaaagcc
     2881 cgatacaaat gaaaatgtag ttcccacaga taatgttgct actacacaac aagttgcaga
     2941 tactgttata gaaaacttag ctgaaaaaat gttaatggct acagaagata atacaaacac
     3001 accagaagac aatactaaaa gtacaccacc tgctaaacaa cattttcata aaaaagaacc
     3061 ggcatttaat attgaatttg atggagttat tacaagcgaa ggtgttttag aaatgatgcc
     3121 agatggttat ggctttttaa gaagtagcga ttataattat ctttcatccc cagatgatgt
     3181 gtatgtttcc ccttctcaaa taaaactttt tggcttaaaa actggcgata cagttacagg
     3241 ctatgtaagg ccaccaaaag aaggagaaaa atattttgct ttactgaagg tagatgccat
     3301 caataacaaa agaccagacg aagtaagaga ccgtgtacct tttgattatt taactccttt
     3361 atttcctttt gagaaattaa atttatttac tacaccacaa agttacagca cacgtgtaat
     3421 ggatttattt acaccaattg gtaaaggaca acgtggatta atagttgctc aaccaaaagt
     3481 gggtaaaacc atgttgctta aagaggtagc caatgccatt gcagccaacc atcctgaatg
     3541 ttatttaatg gtagtgttga ttgatgaaag accagaagag gttactgata tggaaagaag
     3601 tgtaaaggct gaagtgatag catctacttt tgatgaacca gcagaaaaac atgtaaaagt
     3661 atccagcatt gctttacaaa aagcaaaacg tttggtagag tgtgggcatg atgttgtaat
     3721 tcttttagac agtattactc gtttagcccg tgcacataat acagttgcac caagtagtgg
     3781 taaagtgcta agtggtggtg tagaggcaaa tgcaatgcaa aaaccaaaac agttttttgg
     3841 tgcagcccgt aaaattgaaa atggcggctc tttaacaatt ttagcaactg cattaataga
     3901 aactggcagt aaaatggatg aggttatttt tgaggagttt aaaggaacag gtaatatgga
     3961 attgcaatt
//
LOCUS       DAOQPK010000204         3940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_15427_length_3940_cov_3.537452, whole genome shotgun sequence.
ACCESSION   DAOQPK010000204 DAOQPK010000000
VERSION     DAOQPK010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15427_length_3940_cov_3.537452"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..114)
                     /locus_tag="PLU36_08885"
     CDS             complement(<1..114)
                     /locus_tag="PLU36_08885"
                     /inference="COORDINATES: protein motif:HMM:NF012934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HMZ46903.1"
                     /translation="MGSDSDLTVMQAAASFLQQLNIAFELTIVSAHRTPQRL"
     gene            complement(230..844)
                     /locus_tag="PLU36_08890"
     CDS             complement(230..844)
                     /locus_tag="PLU36_08890"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HMZ46904.1"
                     /translation="MLQKNYYKILQVSPQCTQEEIKKAYRKLALQFHPDINHKTTQIF
                     NEIKEAYEVLGNATSRSKYNTTFFYYLVEDKNVNIHSIVQQTYQLTSFIQSGNRFTMD
                     YEMIATYIHHILQPLQVNLIQTTAEKDKYQQIENNLYLTAAALPFHQYQKVYTLLSNV
                     FPNKINLYNKLLKQKKQNWLGEKYAILIAITMTIMLCFIIAYLS"
     gene            complement(883..1461)
                     /locus_tag="PLU36_08895"
     CDS             complement(883..1461)
                     /locus_tag="PLU36_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46905.1"
                     /translation="MYPYIYKYFILNKKLSLPGIGCLIIENCKATFNDNKNAILPPNP
                     TINFSTTTTLADKSFYHFLATELHIEEVDAIKNFHDFSYQLQNGINKANGIVLPGIGT
                     IKKHPSGSLEFETEVDANQYLSEINLSGNKKKTTAAIVAEQQHIFTPPIIDEVNSEDG
                     NNSTQKQTKDYWWIFAILLALIGIVAIYYKLF"
     gene            complement(1471..3225)
                     /locus_tag="PLU36_08900"
     CDS             complement(1471..3225)
                     /locus_tag="PLU36_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ46906.1"
                     /translation="MQKIKNICFIALSFVLLLGNSAYAQKRNTKKINKKTTTKKVVTK
                     KQTAATSKPIKPITTETPKKVNEDTTKPDVVIIYAAFKPSLRNAAKINFTAASPVLDS
                     SKISLKYNVPAQNLFFSYQPVPLKPLALFVDSTNQWVNNQYVKVGYGGYNTPYIETGL
                     SFGNGKSKLTSIYAMHTSSKGSMPFQQFGKTNLNIVGNYIVNNAHELVGSLYYKNNTQ
                     YQYGYQPNTLVFTKKDLKQQFNSIGVVATYKRKMPNQYGITYAPTLHINYFFDAKKAG
                     EFNLIAEMPVTKTFKENFKLNVGLTADITAYKDNIKTLNNNLIFLNAGVQYKLNKVKF
                     NIGLQPAFDNKQVNLLPNITAEAKLKNDKFILVAGFTGKFIKNNYNHLSSYNPWMDQP
                     TFLANTRVLEVSAGFKGSAGQHFTYNASVGYLGFKNMPLFVNNTIDEKSFVMMNDANL
                     QAIKIAGEVGYQIQEKFSFLAGTSIYQFTESANKYAYGILPIEINGSLKWKVLKDLLV
                     KTDVFIWEGPKYLSKNNSTEKLSAAIDINIGAEYSIIPKLNLWLQFNNLFNNKYERWN
                     RYEVLGLNVLAGVVYSFR"
     gene            complement(3235..>3940)
                     /locus_tag="PLU36_08905"
     CDS             complement(3235..>3940)
                     /locus_tag="PLU36_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46907.1"
                     /translation="SKKDYEKALPFYIAVAAKAPNIYAERSALQCARKYYFDKKDYLN
                     AEKYFTQVKSLTMQQENKLEAMRGLLRCQYKQLKWAEALPNAQELLKEKGIAADDKMM
                     ANMIVAKNHQINNNLQEAISAYKLVIQIATSEFSAEAQYRIAEIYLLQNKLNDAEKAA
                     FDVIKKYGSYEYWVTKSYLLLGDIYFIQKDYFNAEATFKSIAENATITELKQEAEQKL
                     AKVIEEKNKTNKVEQP"
BASE COUNT         1226 a          550 c          597 g         1567 t
ORIGIN      
        1 taaacgttgc ggcgttctat gagccgaaac aatagttaat tcaaaagcta tgtttaattg
       61 ttgtaaaaaa cttgctgcag cttgcattac agttaagtcg ctatcgctac ccataactat
      121 acttattatt gttggttgtt tgttttgcat tgattgaact agatttaaaa cttaaaatat
      181 aaatagttta cttatattga attaaccaaa attaattatt aaataacaat taagataaat
      241 atgcaataat aaaacataac ataatcgtca ttgtaattgc aatgagtatg gcatatttct
      301 ctcctaacca gttttgtttt ttttgtttta atagtttatt atacaggttt attttattag
      361 gaaatacatt acttaataaa gtatatactt tttgatattg atgaaatggt agagctgctg
      421 ctgtaagata cagattgttt tctatttgtt ggtacttgtc tttttctgct gtggtttgaa
      481 ttaaattaac ctgtaatggt tgtaaaatat gatgaatata agtggcaatc atttcataat
      541 ccatagtaaa tctattacca gattgtatga agcttgttag ctgatatgtt tgctgtacta
      601 tggaatggat gtttacattt ttatcttcta ccaaataata aaaaaaggta gtattgtatt
      661 ttgacctact tgttgcatta cctaaaactt cataagcttc ttttatttcg ttaaaaattt
      721 gtgttgtttt atggttaata tcaggatgaa actgtagtgc cagttttcta tatgcttttt
      781 ttatttcttc ctgggtgcat tgaggcgata cttgaagaat tttataataa ttcttttgta
      841 gcatttttaa ttactctaca atttaataaa atagattagt acctaaaata atttatagta
      901 aatagctaca atacctatta aagctagtaa aatagcaaat atccaccaat agtctttggt
      961 ttgtttttgt gtactgttat taccatcttc agaattaacc tcatcaataa ttggaggtgt
     1021 gaaaatatgt tgttgctctg ctacaattgc agcagttgtt ttctttttat taccagaaag
     1081 atttatttcg gataaatact gattggcgtc cacctcagtt tcaaattcta aactgccaga
     1141 ggggtgtttt tttattgtgc caataccagg taaaactata ccattagctt tattaatacc
     1201 attttgtaat tgataagaaa aatcgtgaaa attttttatt gcatctactt cttctatatg
     1261 taattcagtt gctaaaaaat ggtaaaaaga tttatcggct aatgttgttg ttgttgaaaa
     1321 attaattgta ggattgggag gtaaaatagc atttttattg tcattaaaag ttgctttgca
     1381 attttcaata attaaacacc caatacctgg taagcttaat tttttattta aaataaaata
     1441 tttataaata taagggtaca ttcacttggt ttatctaaac gaatatacaa cacctgccaa
     1501 tacattcaaa cctaaaactt cataacggtt ccaacgttcg tatttattat taaataaatt
     1561 attaaactgt aaccataaat ttaatttagg tattatgctg tattctgcac caatattgat
     1621 gtctatagca gcacttagtt tttcggtact attatttttg gataaatatt taggaccttc
     1681 ccaaataaaa acatccgttt ttactaataa atcttttaat actttccatt ttaaacttcc
     1741 atttatttct attggcaaaa tgccataagc atatttattg gcactttctg taaactgata
     1801 gatgcttgtt cctgccaaga aagaaaattt ttcttgaatt tgataaccaa cttctccagc
     1861 aattttaatg gcttgaaggt tagcatcatt catcattaca aaagattttt catcaatggt
     1921 attgtttacg aataaaggca tgtttttaaa gcccaagtaa ccaacacttg cattgtaagt
     1981 aaaatgctgg ccagcagatc ctttaaagcc tgcactaact tctaagactc ttgtattggc
     2041 aagaaatgta ggctgatcca tccaagggtt atagctagac aaatggttgt aattattttt
     2101 aatgaacttt ccggtaaagc ctgcaactaa aataaattta tcgtttttaa gttttgcttc
     2161 tgctgtaata tttggtaaaa gatttacttg cttattatca aaggcaggtt gtaagccaat
     2221 attaaatttt accttattta atttgtattg tacaccagca tttaagaaaa ttaaattatt
     2281 atttaaagtt tttatattgt ctttatatgc tgtaatatct gcagttaatc ccacatttaa
     2341 cttaaagttt tctttaaagg ttttagtaac tggcatctct gcaattaaat taaattcacc
     2401 tgctttcttg gcgtcaaaaa aatagttgat atgtaaggtt ggtgcataag taataccata
     2461 ttgatttggc attttacgtt tataggttgc aacaactcca atactgttaa attgttgctt
     2521 taaatctttt ttggtaaaaa ctaaagtatt gggttgatag ccatattggt attgggtgtt
     2581 gtttttataa taaaggctgc caactaactc atgggcatta ttaacaatat aatttccaac
     2641 aatatttaaa ttagttttac caaattgttg aaagggcata ctgccttttg aagaagtatg
     2701 catagcataa atactggtaa gttttgattt gccattacca aagcttaagc ctgtttcaat
     2761 atatggggta ttgtatccac cgtaacctac cttaacatac tgattattta cccattgatt
     2821 tgtagaatct acaaacaatg ctaggggttt tagaggtact ggttggtaag agaaaaatag
     2881 gttttgggca ggcacattat atttcaacga tatttttgaa ctatctaaaa caggtgatgc
     2941 tgcagtaaaa tttattttag ctgcatttct taaagaaggt ttaaaagctg cataaataat
     3001 tactacatct ggcttggtgg tatcttcatt tactttttta ggtgtttcgg tagtaatggg
     3061 ttttattggt ttagatgtgg ctgctgtttg ttttttggta actacttttt tggttgtagt
     3121 ttttttattg atttttttag tatttctttt ttgtgcatag gcactattgc ctaaaagcaa
     3181 aacaaaagaa agtgcaataa aacaaatatt tttaattttt tgcataatag atatttatgg
     3241 ttgttctact ttattggttt tatttttttc ttcaattact ttagctaatt tttgttcagc
     3301 ttcttgtttt aactctgtaa tagttgcatt ttctgcaata cttttaaagg tagcttctgc
     3361 attaaaataa tctttttgaa taaagtaaat gtctcctaaa agaagatagc ttttggtaac
     3421 ccaatactca tacgagccat attttttaat tacatcaaaa gctgcttttt cagcatcgtt
     3481 taatttgttt tgtaataaat atatttctgc aattctgtat tgagcttctg cactaaattc
     3541 agatgtagca atttgtatca ctaatttata tgcagagatt gcttcttgta agttattgtt
     3601 gatttgatgg tttttggcta caatcatatt tgccatcatt ttatcatcgg ctgcaattcc
     3661 tttttctttt aacaattcct gtgcatttgg taatgcttct gcccatttta attgtttata
     3721 ctggcaacgt aataagccac gcattgcttc taacttattt tcttgttgca ttgtaaggct
     3781 tttaacctga gtaaaatatt tttcggcatt gagataatct tttttgtcga aataatattt
     3841 tctggcacat tgcaatgcac tacgttctgc ataaatattt ggtgcttttg cagcaacggc
     3901 aatataaaaa ggcaaagctt tttcataatc ttttttagat
//
LOCUS       DAOQPK010000205         3849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_16066_length_3849_cov_4.274381, whole genome shotgun sequence.
ACCESSION   DAOQPK010000205 DAOQPK010000000
VERSION     DAOQPK010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16066_length_3849_cov_4.274381"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2004)
                     /gene="gcvP"
                     /locus_tag="PLU36_08910"
     CDS             complement(<1..2004)
                     /gene="gcvP"
                     /locus_tag="PLU36_08910"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HMZ46908.1"
                     /translation="MNLFNQQNNEFTNRHIGTTNSTEVAKMLQTIGIESIDELVNKTV
                     PTGIRLNNELNTPPAISEFEYLNELKLVAAKNKVFKTYIGQGYYNTITPSVILRNVFE
                     NPGWYTQYTPYQAEISQGRLESLLNFQTMVCDLTGLPLANASLLDEGTAAAEAMNMLF
                     NAAESDSKKKFFVDEAIFPQTIDVIKTRAIPVQIELVFGDYKNAIIDENYFGAIVQYP
                     NNNGSIEDYSNFIQQAHTVGAGVVMATDLLALTLLKSPGELGADVVVGNAQRFGVPMG
                     FGGPHAGFFAAKEEFKRGMPGRIIGVSIDAQGNRALRMALQTREQHIKREKATSNICT
                     AQALLANMAAMYAVYHGAQGLKDIATRVAMLTQVLNNSLNELGFTNINTCYFDTVIIK
                     ADKNIIQPIAEKNQINFRYFSNGLIGISLDETTTQKDVLDIVYVFAESLGKDEAEIEF
                     DSDLVLTSIPNFAKRTSNYLTHPVFNTHHSESQMMRYIKQLENKDLSLNTSMISLGSC
                     TMKLNAATEMIPVSWAEFGQLHPFLPTNQSEGYQQIIQELSSFLCEITGFDACSLQPN
                     SGAQGEYAGLLTIMGYHIANNNAHKNVVLIPISAHGTNPASAVLAGMKVVVVKSNEDG
                     TIDISDLKAKAEQYKDNLGALMVTYPSTYGVFEEGIKEICSIVH"
     gene            complement(2146..3006)
                     /locus_tag="PLU36_08915"
     CDS             complement(2146..3006)
                     /locus_tag="PLU36_08915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HMZ46909.1"
                     /translation="MEAVKENILLLISTPIYLIIICIELLISNYGSRKLYSLKDTVTN
                     IYLMLLNSIIDLAFRSIYIGVIFTFFYKYRIINNWQSAIWYWLFLLLAEDFLYYWLHR
                     FDHKIRFFWAAHVTHHSSQKMNFTVGFRSSVFQPLYRFIYFIPLPLLGFKPLDIVFMY
                     SATQIWGIFVHTELVRNMGWLEYIFVTPSHHRVHHGSNAKYIDKNMGMFLIIWDKLFG
                     TFQKELDNNEYEPIKYGLTTEITNPNAMNIIFHEWNQIRKDLAQPKLSFKQKWLYIFG
                     KPGYKHHDKS"
     gene            3087..3569
                     /locus_tag="PLU36_08920"
     CDS             3087..3569
                     /locus_tag="PLU36_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HMZ46910.1"
                     /translation="MAIEINENWQKKSAENQKSYKFFLQKADKSKLIKQLPQLHEEAF
                     KTIDCLQCAACCKNYSPRFKTTDIKRISKHLKLKESIFIDTYLTIDTDGDYVLKSAPC
                     SFLGKDNSCTIYEQRPSDCKRFPYTDEDVLLKRTNLTLKNASFCPAVYYVLESLIQKK
                     "
BASE COUNT         1365 a          602 c          527 g         1355 t
ORIGIN      
        1 atgtactata ctacaaattt cttttatacc ttcttcaaat acaccataag tactagggta
       61 agtaaccatt aatgctccta aattatcttt atattgttct gcttttgctt tcaaatcaga
      121 aatgtcgata gtgccatctt cattactttt tactacaact actttcatac ctgctaaaac
      181 tgcactagct gggtttgtgc catgtgcaga aattggaatt aataccacat ttttatgagc
      241 attattatta gcaatatgat aacccattat agttaataat cctgcatatt ctccttgtgc
      301 accactatta ggttgaaggc tacaagcatc aaagcctgta atttcacata aaaagctgga
      361 gagttcttga attatttgtt gatacccttc tgactgattt gttggaagga aaggatgtaa
      421 ttgaccaaac tctgcccaac taacaggtat catttctgtt gctgcattta atttcatagt
      481 acagctacct aatgaaatca tgctagtatt aagagataaa tctttgtttt ctaattgttt
      541 tatataacgc atcatttgac tttcactatg atgggtatta aaaactggat gtgttaaata
      601 attagaagtt ctttttgcaa aatttggaat agaggttaat accaaatcac tatcaaattc
      661 aatttccgct tcatcttttc ctaaactttc tgcaaataca tacacaatat ctaaaacgtc
      721 tttttgggtg gttgtttcat caaggctaat tccaattaat ccgttggaaa aatatctaaa
      781 attaatctga tttttttctg ctattggttg aataatgttt ttatctgctt taatgataac
      841 agtatcaaaa taacaagtat tgatattagt aaagcctaac tcatttaatg aattatttaa
      901 aacttgtgta agcattgcca ctcttgtagc aatatctttt aatccctgtg caccatgata
      961 aacagcatac atagcagcca tattggctaa taaagcttga gcagtacaaa tattactagt
     1021 tgctttttct ctcttaatat gttgctctct tgtttgtaaa gccatacgta aagctctgtt
     1081 accttgtgca tctatactta ctccaattat tctaccaggc ataccacgtt taaactcttc
     1141 ttttgctgca aaaaaacctg catgtggacc gccaaagccc ataggaacac caaaacgttg
     1201 tgcattacca acaacaacat cagctcctag ctctcctggt gattttaata aagttaatgc
     1261 taataaatct gtagccatta ctacacctgc accaactgta tgagcctgtt gaataaaatt
     1321 gctataatct tctatagagc cattgttatt tggatattgt acaattgcac caaaataatt
     1381 ttcatcaatt attgcatttt tataatctcc aaaaactaat tcaatttgca caggaatagc
     1441 ccttgttttt ataacatcta ttgtttgagg aaatattgct tcgtccacaa aaaatttctt
     1501 ttttgaatcg ctttcagcag cattaaataa catgttcatg gcttctgcag cagcagttcc
     1561 ttcatctaat aatgaagcat tggctaatgg caatcctgtt aaatcgcaaa ccattgtttg
     1621 aaagtttaat aaactttcta atctaccctg agaaatttct gcctgataag gagtatattg
     1681 tgtgtaccag cctggatttt caaaaacatt tcttaaaata acactaggtg taatagtgtt
     1741 ataataccct tgtccaatat aagttttaaa aaccttattt tttgctgcaa ctaatttaag
     1801 ttcatttaaa tattcaaact cgctaattgc aggtggtgta tttaattcgt tgtttaacct
     1861 aatgccagta ggcactgttt tattaactaa ctcatcaata ctttcaatac caattgtttg
     1921 taacatttta gctacttctg tagaatttgt tgttccaata tgcctgttag taaattcgtt
     1981 attttgctga ttaaataagt tcataaaaaa tatattttta aatagtgagg tcttaaaaaa
     2041 ttgcggcaaa tgtacgagtt cctattttat ttttaattga ttgttatagg tatagaaaaa
     2101 gttgttatta aatgtaaaaa aagaaaattt atttctacaa aaaaatcaac tcttatcatg
     2161 gtgtttatac cctggtttcc caaaaatata aagccatttt tgtttaaagg ataatttagg
     2221 ctgagccaaa tctttacgaa tttggttcca ttcatgaaaa ataatattca ttgcatttgg
     2281 gttggtaatt tcagtagtta aaccatattt aattggttca tactcattat tatctaattc
     2341 tttttgaaat gtaccaaaaa gtttatccca aataattaaa aacattccca tgtttttatc
     2401 aatgtatttt gcattgcttc catgatgtac tcgatgatga gatggtgtaa caaatatata
     2461 ctctaaccaa cccatatttc taactaattc ggtatgtaca aaaatgcccc aaatttgagt
     2521 agcagaatac ataaatacaa tgtctaaagg tttaaaacca agtaagggta atggaataaa
     2581 ataaataaac ctatatagtg gctgaaaaac agatgaacga aaacctactg taaaattcat
     2641 tttttgagaa gagtgatgcg ttacatgagc tgcccaaaaa aatcttattt tatgatcaaa
     2701 cctatgtagc caataatata aaaaatcttc tgctaataat aaaaaaagcc aataccaaat
     2761 agctgattgc caattattaa ttattctgta tttataaaaa aaagtaaaaa taactccaat
     2821 ataaatgctt ctaaaagcta aatcaataat actatttaat agcattaaat aaatattggt
     2881 aactgtatct tttaaggaat acaattttct actaccatag ttagatataa gtaattcaat
     2941 acaaataatt attaaataaa ttggtgtaga aattagtaag agaatatttt ctttaactgc
     3001 ctccataata aaacaaagta atcaatttta taataaagtg agaatgaaaa ttaatgttta
     3061 actagtttaa ataaatacat ttgcctatgg caattgaaat aaatgaaaat tggcagaaaa
     3121 aaagtgccga aaaccaaaag agttataaat tttttttaca aaaagcagat aaaagtaagc
     3181 ttataaagca actgcctcaa ttacatgagg aagcttttaa aacaatagat tgtttgcaat
     3241 gtgctgcatg ttgtaaaaac tattcaccac gttttaaaac aactgatatt aaaagaatta
     3301 gtaagcattt aaaactaaaa gaaagcattt ttatagatac ttatctaaca attgatactg
     3361 atggagatta tgtactaaaa tctgctcctt gtagtttttt aggtaaagat aattcttgta
     3421 ctatttacga gcaacgtcct agcgattgta aacgatttcc ctatactgat gaagatgtgt
     3481 tattaaaacg taccaattta acacttaaaa atgcatcatt ttgtccggca gtgtattatg
     3541 ttttagaaag tctaattcaa aaaaagtaaa ttattctatt taccagcttc aaattgtaga
     3601 ttatatatac tgattagatg catctcataa aaaacttggt ttgggttatt tatatgctac
     3661 ctgttataat ggaacttaaa gtataataga taccactttg ccagtaataa ttaacctttc
     3721 tgccaattct ttataagaga aaataatcaa tgtttattta atagaaaaga aggtggaatg
     3781 acaggttcaa taacttctac tttaagttac tatgagttta ctaaaactag agaaatactt
     3841 gcagatgag
//
LOCUS       DAOQPK010000206         3833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_16196_length_3833_cov_4.305982, whole genome shotgun sequence.
ACCESSION   DAOQPK010000206 DAOQPK010000000
VERSION     DAOQPK010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16196_length_3833_cov_4.305982"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..1340)
                     /locus_tag="PLU36_08925"
     CDS             complement(183..1340)
                     /locus_tag="PLU36_08925"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HMZ46911.1"
                     /translation="MSIILFDTPIRKNFYPITLNKSLGDVRLGIFTLKEWWQQALNKP
                     ILLKTDDYLQPLYEEINDSEEHLFIDASVVPDNNIIHKILALQSSEAIADEFGLIAFK
                     TSNFNNTLSEIFKKIKIIDNRNFVNRITQPFHLFQINEQILKEQFDWIIKQKQSYKIS
                     STNVVINPENIFIDEEANVEHCILNAQNGIIYVGKNATIMEGSIIRGSCAILENAVIK
                     AGTKIYGATTIGKFAVASGEIKNSIISDYSNKAHDGYLGDSFIGSWCNIGAGTNNSNV
                     KNNFNEIFLYKEATNEKVNAGKKCGVIMGDFTRVAINSAINTGSVYGISCNVFGNGLL
                     PKTISNFSWGLNDKYKYSKVIEDINNWMYFKGKQLSDAEIIVLKYIFDNNL"
     gene            1632..>3833
                     /locus_tag="PLU36_08930"
     CDS             1632..>3833
                     /locus_tag="PLU36_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HMZ46912.1"
                     /translation="MNFKKVNNITGWIIALIACTVYIVTAEAGGSLWDCGEFVSSCFK
                     LQVPHPPGAPMFVILGRFFIVLLGNDPLTAAKAVNIMSALASGFTILFLFWTITHFAR
                     RIVHSDVSKPLTQTQILSIMGAGVVGALAYTFSDSFWYSAVEGEVYALSSFFTALVFW
                     AILRWEHEADQKGADRWIVFIFFMMGLSIGVHLLNLLTIPAIVMLYYIRKRIDFNYKI
                     IRKYFILFTIIGGSLAFIGTLIVANSEADRVPLDATLAGLALVGTALVIVFLRLVESF
                     KKEKKEYYGGMYIFFILGCIITGVVQVGVIQYTVKMAGAFDRYFVNGFGMPFFSGFAF
                     FFILLAALIWYGLKIAAKKGWYYLKLSLWCVTFMLIGYSTYVTTMIRSNADPAIDMYN
                     VDNPMSLVGYLGREQYGDFPILYGQKFTARPYDSKETGTKYQKGKDNYVEIGKDFKYI
                     YAAEDKMVFPRVWDASNDQSHADYYASFLNIGQMQDGTYERSPNQIDNMHFFLGYQIN
                     FMYFRYFMWNFSGKQNDIQGSSLANLRDGNWITGINFIDNLRLGNQDKMPDSLTHNKA
                     NNKLYALPFILGLIGLLYHFKQKGADALVNMLLFFFTGLAIVVYLNQAGNQPRERDYA
                     YVGSFYAFAVWIGLGVLQVKSWITRVVNQKVAATLATVICLLAVPVLMAQQEWDDHDR
                     SNKTLARDLAKDYLESCPKDAILFCFGDNDTYPLWYAQEVEGIRTDVRVVNL"
BASE COUNT         1232 a          538 c          642 g         1421 t
ORIGIN      
        1 atttatcttt agctaacaaa aggtatggag ctggtacacc aataatgctt attttatttt
       61 ctgtggtgtt tactgttgca gtacttaatt tagttaataa ttcttctgct tgttgtaaag
      121 tgcaattcat tttccagtta gcagcagcaa tttgtttacg catgagtttt aaatttatta
      181 agttataagt tgttgtcaaa gatatatttc aaaacaatta tttcagcgtc cgataattgt
      241 ttgcccttaa aatacatcca attattaata tcttcaatta ctttagagta tttatattta
      301 tcgtttaatc cccaagaaaa attagagatg gttttaggca acagcccatt cccaaaaaca
      361 ttgcaactaa taccgtaaac agagcctgta tttattgcag aattaatagc aactctagta
      421 aaatctccca taattacacc acattttttt ccagcattaa ctttttcatt tgttgcctct
      481 ttataaagaa aaatttcatt gaagttgttt tttacattgc tattgttagt tcctgctcca
      541 atattgcacc aactaccaat aaaactatca cctaaataac catcatgggc tttattacta
      601 taatcgctga tgatgctatt tttaatttct ccacttgcca cagcaaattt accaatggtt
      661 gtagcaccat aaatttttgt acctgcttta ataactgcat tttctaaaat agcacaacta
      721 cctctaataa tactaccttc cattattgta gcatttttgc caacataaat aattccattt
      781 tgggcattta aaatacaatg ctctacatta gcctcttcat ctataaaaat attttctgga
      841 ttaataacaa cattagtgga agaaatttta taagattgtt tttgtttaat aatccaatca
      901 aattgctctt tcaaaatttg ttcatttatc tgaaataaat gaaatggttg agttattcta
      961 ttgacaaaat tcctattatc tataatttta atttttttaa atatttcaga caaagtgtta
     1021 ttaaaattac tagttttaaa agcaattaat ccaaactcat ctgcaatagc ttcagatgat
     1081 tgcagagcaa gtattttgtg aatgatatta ttatctggta ctacagatgc atcaataaat
     1141 aaatgttctt cactgtcatt tatttcttca taaagtggtt gtaaataatc atcagttttt
     1201 agtagtattg gtttattgag agcctgttgc caccactctt ttaatgtaaa aatacctaaa
     1261 cgtacatcgc ctaaagattt atttagtgta atagggtaga agttttttct tataggtgta
     1321 tcaaataaaa taattgacat gatggaaggc aagataataa aaaatgtaat tagaaagttg
     1381 gattagttaa gaagtaaaaa ttattccttt tatgaaataa tttcattaaa attttttttt
     1441 tgaaaattaa tcatacagca acaaatagtt aactgcgata gttgatttct gacaggtaaa
     1501 tcaattattc tttaatttgt gcaatattca aactagtact tgttgttaca gcttttttaa
     1561 actttttcaa ctgttttgct aattttcctt ttattcattt atgtttgctg cactctttaa
     1621 aataaaaacc aatgaatttt aaaaaagtaa ataatattac gggatggatt attgccctaa
     1681 ttgcttgtac tgtttatatt gtaactgctg aggcaggggg gagtttatgg gattgtggag
     1741 aatttgtaag tagttgtttt aaacttcaag tacctcaccc tccaggtgcc ccaatgtttg
     1801 ttattttagg tagatttttt attgtacttt tgggtaacga ccctttaact gcagcaaaag
     1861 cagttaatat tatgagtgct ttggcaagcg gttttaccat cttattttta ttttggacca
     1921 ttacccactt tgcaagaaga attgtacatt ctgatgtaag taaaccttta acccaaaccc
     1981 aaattttatc tataatgggt gcaggtgttg taggtgcttt agcttatact tttagcgata
     2041 gcttttggta cagtgctgtt gaaggggaag tgtatgctct tagttctttc tttactgctt
     2101 tagttttttg ggcaattttg agatgggaac atgaagcaga ccaaaaagga gcagaccgtt
     2161 ggatagtttt tatatttttt atgatgggct tatctattgg ggttcactta ttaaacttac
     2221 ttactattcc tgcaattgta atgctgtatt atatcagaaa aagaattgat tttaattaca
     2281 aaattattcg taagtatttt atattattta ccattattgg aggttcattg gcttttattg
     2341 gtactttaat agttgccaat agcgaggcag atagagtgcc tttagatgca actttggctg
     2401 gtttagcttt agttggtaca gctttagtaa ttgtattctt aagacttgta gaatcattta
     2461 aaaaagaaaa gaaagaatac tacggaggta tgtatatctt ttttatttta ggttgtatta
     2521 taactggtgt tgtacaagtt ggggtaatac aatatacagt taaaatggct ggtgcttttg
     2581 atagatattt tgtaaatggt tttggtatgc cattctttag tggctttgca ttcttcttta
     2641 ttttattagc agcattaatt tggtatggat taaaaattgc tgcaaaaaaa ggatggtact
     2701 acttaaaatt aagtttatgg tgtgttacat ttatgcttat tggttatagt acttatgtaa
     2761 ccacaatgat tcgtagtaat gcagatcctg ctattgatat gtacaatgta gataacccaa
     2821 tgagtttagt gggttattta ggacgtgaac aatatggcga ttttccaatt ttatatggtc
     2881 aaaaatttac agcacgtcct tacgactcta aagaaacagg tacaaaatat caaaaaggga
     2941 aagacaacta tgtagaaatt ggaaaagact ttaaatacat atatgcagca gaagataaaa
     3001 tggtttttcc aagagtgtgg gatgcaagta atgatcaatc tcatgcagat tattatgcca
     3061 gctttttaaa cattggccaa atgcaagatg gtacgtatga aagaagccct aaccaaatag
     3121 ataatatgca cttcttttta gggtatcaaa ttaatttcat gtacttcagg tattttatgt
     3181 ggaatttttc tggcaaacaa aatgatattc aaggttcttc cttagccaat ttaagagatg
     3241 gtaattggat aacaggaatt aattttattg ataacctacg tttaggtaat caagataaaa
     3301 tgccagatag tttaacccat aacaaagcaa ataataaact ttatgcttta ccttttatac
     3361 tagggctaat tggtttattg tatcatttta aacaaaaagg tgccgatgct ttagtaaata
     3421 tgctattatt cttctttaca gggcttgcaa tcgttgttta cttgaatcag gctggtaacc
     3481 aaccaagaga gagagattat gcgtatgttg gtagttttta tgcatttgct gtttggattg
     3541 ggttaggtgt attgcaagta aaatcctgga ttactcgtgt tgtaaaccaa aaagtggcag
     3601 caactttggc taccgttatt tgtttattgg cagtacctgt tttaatggct caacaagagt
     3661 gggatgatca tgatagaagc aacaaaacac ttgcaagaga tttagcaaaa gattacttag
     3721 aaagttgccc taaagatgcc atactttttt gttttggaga taatgatact tacccattat
     3781 ggtatgcaca agaagtagaa ggaatcagaa cagatgtgcg cgtagtaaat tta
//
LOCUS       DAOQPK010000207         3825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_16243_length_3825_cov_6.003183, whole genome shotgun sequence.
ACCESSION   DAOQPK010000207 DAOQPK010000000
VERSION     DAOQPK010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16243_length_3825_cov_6.003183"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /locus_tag="PLU36_08935"
     CDS             complement(<1..138)
                     /locus_tag="PLU36_08935"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMZ46913.1"
                     /translation="MNILLTGANGYIGMRLLPVLVESGHEVTCVVRDKNRFKPAKDLL
                     DK"
     gene            complement(149..1132)
                     /gene="moaA"
                     /locus_tag="PLU36_08940"
     CDS             complement(149..1132)
                     /gene="moaA"
                     /locus_tag="PLU36_08940"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HMZ46914.1"
                     /translation="MLTDKFNRTHTYLRISITDKCNLRCTYCNPIDLPKGYFSVSNKM
                     SPDEIEQIATTFVKNGITKIRLTGGEPLVRKDAKEIILRLAKLNVELAITTNGVYVNE
                     FIEVFKQANIKTVNVSLDTLKQQKNLLITGRNEFDKVHENINLLLQNDFTVKVNVVVM
                     KNTNDNEILDFVEWTKNQPVHVRFIEFMPFSGNNWSNDKVFNYKEILQLIKTKYKYEN
                     LQQDKSDTAMKFKAVNHKGTFAIISTMSKPFCGGCNRLRLTTDGKMKNCLFSKTEVDI
                     LTALRNKENIEPLIQQCVWDKEEIQGGQFNSINGINDALAIKNRSMIFIGG"
     gene            complement(1148..1849)
                     /locus_tag="PLU36_08945"
     CDS             complement(1148..1849)
                     /locus_tag="PLU36_08945"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HMZ46915.1"
                     /translation="MNNIFNNGNKDNSDYIKRFVEKDTGDVQVYSPMDPPDAYKPQGV
                     EPVPYNELHAMMQALMDEHKITLEKINDFEATLLSIKKDGVNKESNKKLGDFFAFIDE
                     KIVLHNLKEERILFPLIHDRMIDNGEHGTGKIPDTAVDLLENDHIKMMELATLTFSLM
                     GITSRITDPISNALLMDTAIEQGLSLVEMLRLHIFREDNVVFPLAHKYLTPSDYEKIT
                     HQMKKYFSIDLPSKN"
     gene            complement(1859..2944)
                     /locus_tag="PLU36_08950"
     CDS             complement(1859..2944)
                     /locus_tag="PLU36_08950"
                     /inference="COORDINATES: protein motif:HMM:NF015552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HMZ46916.1"
                     /translation="MKENKSIQISTTIKEGIKTLSPAYFALVMATGIISIGAYIMQFK
                     WIATTLFFLNNIFFVLLWALTILRVVWYFKFLKTDLTNHLTGTGFFTTVAGTCVLGIQ
                     YALMYERFTIATVLLVWGILLWLFLTYTIFTTLTTQQNKPTLDKGITGGWLTAIVATQ
                     SVSILSALVATHIGQPYRVEINFFALSMWLWGGMFYIWMISLIFYRYTFFKFSPDDLA
                     PPYWINMGAMAISALGGSVLILNAPGAPFLDSLLPFIKGFTVFYWATGTWWIPMLIIL
                     AIWRHIIKRYPLKYDPLYWGAVFPQGMYAVCTYRMSKALNMPFLHYFYQAFIYLAIIA
                     WTITFLGLLKFLYSKLVNQNHFILKKK"
     gene            complement(2978..>3825)
                     /locus_tag="PLU36_08955"
     CDS             complement(2978..>3825)
                     /locus_tag="PLU36_08955"
                     /inference="COORDINATES: protein motif:HMM:NF013473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ46917.1"
                     /translation="KEVRVWRFGLYYFFVFGAFVALSQWLIPYYVNVYSLSIVSAGFM
                     AAAFSLPAGLFRAAGGWLSDKVGARMLLIWTLSVCLICMIFLFIPRMEIQAPGQGIMS
                     KKAGKVMAVSNSEILIGSDVYTLQDKAGDASNVTIRFGIHHDQEGFLFLPTTTFHQVP
                     NVKVGEEVKKGDLIAKGVSLIYFQANKWIFTTLVFIIGIMMGLGGAAVFKHISDYYPN
                     NIGTVGGIVGVLGGLGGFCGPIIFGFLLKQTGIWTSCWMFLAALVMLCIVLQRTAIKA
                     LLKNN"
BASE COUNT         1283 a          675 c          506 g         1361 t
ORIGIN      
        1 tttatctaat aaatcttttg ctggtttaaa cctgttctta tctcttacca cacaagtaac
       61 ttcatgaccc gactctacca atactggtaa taacctcatt cctatataac cattagctcc
      121 tgtaagtaat atattcatac gctattgttt aacctccaat aaaaatcata cttctatttt
      181 taattgctaa agcgtcattt attccattta tagaattgaa ttgtccccct tgtatttctt
      241 ccttatccca aacgcattgt tgaattagtg gttcaatatt ttctttgttt cttaatgctg
      301 ttaaaatatc tacctcagtt ttagagaaca aacagttttt catttttcca tcggtagtta
      361 atcttagtct attacaacca ccacaaaatg gcttactcat tgtactaata attgcaaatg
      421 tgcctttatg gtttactgct ttaaatttca ttgcagtatc acttttgtct tgctgtaggt
      481 tttcgtattt atattttgtt ttaataagtt gaagtatttc tttataatta aatactttat
      541 cattactcca attatttccc gaaaatggca taaactctat aaacctaaca tgaacaggtt
      601 ggttttttgt ccattctaca aaatctaaaa tttcgttatc attagtattt ttcattacta
      661 ctacatttac ttttactgta aaatcatttt gtagtagaag gttaatattt tcatgtactt
      721 tatcaaactc atttctgcct gtaattaata aatttttttg ttgctttaat gtatctaaac
      781 ttacattaac ggttttaata tttgcttgtt taaatacttc aataaattca ttaacgtaaa
      841 cgccattggt tgtaattgcc aattcaacat ttaatttagc taatcgtaaa ataatttctt
      901 ttgcatcttt tcttactaag ggttcgccac ctgtaagcct tattttagta attccgtttt
      961 ttacgaaagt ggttgcaatt tgttcaatct catcggggct cattttatta gatacagaaa
     1021 aatatccttt tggtaaatca ataggattac aataggtgca acgtaaattg catttatctg
     1081 ttatagaaat tcttaaataa gtatgtgttc tattaaattt atcggttagc ataactctat
     1141 attataatca atttttagag ggcaggtcta tagagaagta ctttttcatt tgatgtgtaa
     1201 ttttttcata atcgcttggt gttaggtatt tatgggctaa tggaaaaact acattgtctt
     1261 ctctaaagat atgcaatctt agcatttcta ccaaagacaa gccttgttct atagcagtat
     1321 ccatcagcaa tgcattagat ataggatctg taattctaga agttataccc attaagctaa
     1381 aagttaaggt tgccaactcc atcattttta tatgatcatt ctctaacaaa tctactgctg
     1441 tatctggtat ttttcctgtt ccatgttctc cattatctat cattctatca tgaattaatg
     1501 gaaataaaat tctttcttct tttaaattat gaagtacaat tttttcatca ataaatgcaa
     1561 aaaaatctcc aagcttttta ttgctttctt tattcactcc atcttttttt atggataata
     1621 atgtagcttc aaaatcatta attttttcaa gtgttatttt atgttcatcc attaatgctt
     1681 gcatcatagc atgtaattca ttatagggta ctggttctac accttgtggt ttgtaagcat
     1741 cgggtgggtc cattggtgag taaacttgta catctcctgt atctttttct acaaatcttt
     1801 taatataatc gctattgtct ttatttccgt tgttaaaaat gttgttcata gtttgtattt
     1861 attttttttt taaaataaaa tgattttggt ttaccaattt agaatataaa aactttaata
     1921 atcctaagaa ggttattgtc caggcaataa ttgccaagta aataaatgcc tgataaaaat
     1981 aatgcaaaaa gggcatattt aaagctttag acattcgata ggtgcaaact gcatacatgc
     2041 cttgcggaaa cacagctccc caatataaag gatcgtattt taagggataa cgtttaataa
     2101 tatgtcgcca aatggctaat ataatcagca ttggaatcca ccatgtgcca gttgcccaat
     2161 aaaaaactgt gaagcctttt ataaaaggca ataacgaatc taaaaaaggt gctcctggtg
     2221 catttaaaat taaaacactt cctccaagtg ctgatattgc cattgcaccc atatttatcc
     2281 aataaggagg agctaaatca tcgggagaaa atttaaaaaa agtatatcta taaaagatta
     2341 aagaaatcat ccagatataa aacatgcctc cccaaagcca catagataat gcaaaaaaat
     2401 taatttcaac tctgtagggt tgaccaatat gcgtagctac taacgcactt aaaatagaaa
     2461 ccgattgtgt ggcaacaatt gctgtaagcc aaccacccgt tattccttta tctaatgtag
     2521 gtttattttg ttgtgttgtt aatgttgtaa aaatggtata ggttaaaaaa agccataata
     2581 aaatacccca aactaataaa actgttgcaa ttgtaaatcg ttcatacatt aaagcatact
     2641 gtattcctaa aacacaagtt cctgcaactg ttgtaaaaaa acctgtgcct gtaaggtgat
     2701 tggttaaatc ggtttttaaa aatttaaaat accatacaac tcttaaaata gttaatgccc
     2761 ataatagcac aaagaaaata ttgttaagaa aaaagagagt agtagcaatc catttaaact
     2821 gcataatata tgcaccaata gagataatgc ctgttgccat tactaaagca aagtaagcag
     2881 gagaaagtgt ttttatacct tcttttattg ttgtactaat ttgtatgctt ttattctctt
     2941 tcatttataa ttagtaacat tcttttgttt attgctatta attatttttc agtaaggcct
     3001 ttattgctgt acgttgtaat acaatacata gcataactaa cgctgctaaa aacatccagc
     3061 aagaagtcca aatgcctgtt tgttttaata aaaaaccaaa aattattggg ccacaaaatc
     3121 ctcctaaccc acctaaaact cctacaatac caccaacagt tccaatatta ttaggatagt
     3181 agtctgaaat atgtttaaat actgctgcac ctcctaaacc catcattata ccaattataa
     3241 aaaccagtgt ggtgaaaatc catttatttg cctgaaaata aataagagaa accccttttg
     3301 ctattaaatc tcctttctta acctcctcac caacttttac atttggtacc tgatgaaaag
     3361 tagttgttgg taaaaacaaa aaaccttcct gatcatgatg aataccaaac cttatagtaa
     3421 cattggatgc atcacctgct ttgtcttgaa gggtgtacac atcgcttcca attaatattt
     3481 cagagttgga aacggccatt actttaccag cttttttact cataatgcct tgtcctggtg
     3541 cttgtatttc cattctaggt ataaataaaa aaatcataca aataagacaa acactcaatg
     3601 tccaaattaa taacattctt gcacctactt tatccgataa ccagccacct gctgccctaa
     3661 acaagcctgc tggcaaacta aaagctgcag ccataaaacc agcagataca atagataaag
     3721 aataaacatt tacatagtaa ggaattaacc attgagacaa tgcaacaaaa gctccaaaaa
     3781 caaaaaagta gtataaccca aaacgccaaa cccttacttc tttta
//
LOCUS       DAOQPK010000208         3825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_16244_length_3825_cov_3.724934, whole genome shotgun sequence.
ACCESSION   DAOQPK010000208 DAOQPK010000000
VERSION     DAOQPK010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16244_length_3825_cov_3.724934"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1747
                     /locus_tag="PLU36_08960"
     CDS             <1..1747
                     /locus_tag="PLU36_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HMZ46918.1"
                     /translation="KNRFPKSDMLNAKEAGTWKHYSTSNIETIVNKFSAGLLKLGVQC
                     NDCTAEGSDKIAIISNNRPEWLFTDFAVQQIGAILVPVYPTTNPLELEFILNDAQIKY
                     VFASSEELYLKAKSVASKVPSVKNIYTFDKVENAPYYLEVLELANEELLQNVEKIKKG
                     IPVTHTATIIYTSGTTGTPKGVMLSHENIVSCTFLSKVSFPFEDAPQSKVLSFLPLNH
                     IFEKCVSYIYLFSGISIYYAESLETIGENLKEIQPDGFTTVPRLLEKVYEKIMLKGNE
                     LTGIKRKLFFWSVAVASKYDNLKSGGLLYNIKLAIARKLVFKKWREALGGNIQYIVTG
                     GAACQENLLRIFNGAGIPVYEGYGPTENSPVISVNRKEKGGSMYGSVGPIIEGLEVKL
                     AEDGEILVKGPTVMQGYYKRPDLTAEVLKENWLYTGDIGVWVNNKFLKITDRKKELFK
                     TSGGKYVAPQPIENKLKENRFIEQVIIVGAEKKFVGALIVPSFSALSEWMKNNNINVS
                     SNNEAIVNPAVLNLYKEIVEEYNQYFNHVEQVKKFELLPNEWSIDTGEMTPKMSLKRK
                     IILEKYKSSIDRIYN"
     gene            1822..2556
                     /locus_tag="PLU36_08965"
     CDS             1822..2556
                     /locus_tag="PLU36_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HMZ46919.1"
                     /translation="MELDPTETAIAEPGAFMMMHDGIQLETIFGDGSQQQGGLLGKLF
                     SAGKRMLVGESLFMTAFTNVSSTKKSVSFASPYPGKIVTIDLMRIGGKVICQKDAFLC
                     AAKGVKIGIEFQRKLGTGLFGGEGFIMENLEGDGMAFLHASGFVQEIDLQQGELLKID
                     TGCIVGFTATVDYDIQFVGGIKNTLFGGEGLFFATLKGPGRIWVQSLPISRLAGRILA
                     YGTYKRKEEGSILGGIGNILDGDGWK"
     gene            complement(2590..3678)
                     /locus_tag="PLU36_08970"
     CDS             complement(2590..3678)
                     /locus_tag="PLU36_08970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ46920.1"
                     /translation="MQENNTYKIVQIIDVLDAGGAEKVLITLSNLLHKHGHKVTVITT
                     VRKGIIANQLQEGIEIKELNRKHKWHLKSMQQLVQWCKHADIVHVHSAHNLRFLFVAA
                     AFFGLRKKIFFHEHFGNIDVDKRVHWHQKIIFPKVVFIAVSKSLYQWALENLKLKKEN
                     TFLLENIIIKLPIEKIEKIDTDKKHLVMVSNFRPPKNIEFAIELMKFLPSHFHLTILG
                     QPAYTEYLTSIINQIEENSLQHKVTIEHNCSNVQSILPKFDMAIHTANTESGPLALIE
                     YMAQQIPFITYNTGEVVQQIKPHLGELVMNDFDISNWVKKLLAIIENDNSALKEKLKT
                     TFEEFYSEEAYYEKCMAIYKTALKNKKA"
BASE COUNT         1354 a          501 c          674 g         1296 t
ORIGIN      
        1 aaaaaatagg ttccccaaaa gcgatatgct taatgccaaa gaagcaggca cttggaagca
       61 ttatagcaca tccaatatag aaacaattgt aaataaattc tctgcagggc ttttaaaact
      121 tggggtacaa tgcaatgatt gcacagccga aggaagtgat aaaattgcta tcattagtaa
      181 caacaggcct gagtggttat ttacagattt tgcagtgcaa caaattgggg caattttagt
      241 tcctgtttac cctactacaa accctttaga gttagaattt attttgaatg atgcccaaat
      301 aaaatatgtt tttgctagta gtgaggaact gtacttaaaa gcaaagtcgg tagcaagtaa
      361 agttccttct gtaaaaaata tttacacttt tgataaagta gaaaatgcac cttattatct
      421 ggaggtttta gaactagcca atgaagaact tttacaaaat gtagaaaaaa taaaaaaagg
      481 aattcctgtt acacatacag caacaattat ttatacatct ggcacaacag gtacgcccaa
      541 aggggttatg cttagccacg aaaatattgt aagttgtaca tttttatcta aagtaagttt
      601 tcctttcgaa gatgcaccac aatcaaaagt actaagcttt ttaccactta accatatttt
      661 tgaaaaatgt gtaagttata tttatttatt tagcggaatt agtatttatt atgctgaaag
      721 tttagaaacc attggcgaaa atttaaaaga aattcaacca gatggtttta ctactgtgcc
      781 aaggctatta gaaaaagtgt acgagaaaat aatgttaaaa ggaaatgaac ttacaggcat
      841 taaaagaaaa ttattttttt ggagtgtggc agtagcatct aaatacgata acttaaaaag
      901 tggaggcctt ttgtacaata taaaattagc tatagctcgt aaattagttt ttaaaaaatg
      961 gagagaagca ttaggtggca atattcaata cattgttact ggtggtgcag cttgtcagga
     1021 aaatttatta agaatattta atggtgctgg cattcctgtt tatgaaggat atggccctac
     1081 agaaaacagc cctgttatta gtgttaacag aaaagaaaag ggaggctcaa tgtatggctc
     1141 agtgggtcca attattgaag gattagaagt aaaacttgcc gaagatggag aaatattagt
     1201 aaaaggccca acagttatgc aaggctatta caaacgcccc gatttaacgg ctgaagtttt
     1261 aaaagaaaat tggctataca caggtgatat aggagtttgg gtaaataata aattcttaaa
     1321 aataacagat agaaaaaaag agctatttaa aacaagtgga ggaaaatatg ttgcccctca
     1381 accaatagaa aataaattaa aagagaatag atttattgaa caagtaatta ttgtaggtgc
     1441 cgagaaaaaa tttgtaggtg ccttaattgt tccttcattc tctgcattaa gtgaatggat
     1501 gaaaaataat aatattaatg tttcatcaaa taacgaagca attgtaaacc cagctgtttt
     1561 aaatttgtat aaagaaattg tagaagagta taaccaatat tttaatcatg ttgaacaggt
     1621 taaaaaattt gaattattac ccaatgaatg gagtattgac actggtgaaa tgactcctaa
     1681 aatgagctta aaacgaaaaa ttattttaga aaaatacaaa tcatctattg acagaattta
     1741 taattaatta aaatattttt tatgagaggc aatcatgaaa ttgattacag aatttttggg
     1801 gaagaaatgc aatatgtata aattgaatta gaccccactg aaacagcaat tgcagagcca
     1861 ggtgcattta tgatgatgca tgatggcata caattagaaa ccatttttgg cgatggcagt
     1921 caacaacaag gtggtttatt aggtaaatta tttagtgcag gtaaacgaat gttggtgggc
     1981 gaaagtttgt ttatgactgc atttaccaat gtaagcagta ctaaaaaaag tgtaagtttt
     2041 gcttctcctt atcctggtaa aattgtaacc attgatttaa tgcgaatagg tggtaaagtt
     2101 atttgccaaa aagatgcatt tttatgtgct gcaaaaggag ttaaaattgg tattgaattt
     2161 caaagaaaat taggtacagg tttatttggt ggtgaaggtt ttattatgga aaatttagaa
     2221 ggcgatggaa tggctttttt acatgccagt gggtttgtac aagaaataga tttacaacaa
     2281 ggtgaattac taaaaataga tactggctgt attgttggtt ttacagcaac cgttgattat
     2341 gatatacagt ttgtaggagg tattaaaaat actttatttg gtggcgaagg attatttttt
     2401 gcaacattaa aaggcccagg aagaatttgg gtacaaagtt tacctattag ccgtttagct
     2461 ggtagaattt tggcttatgg tacttataaa cgcaaagaag aaggtagcat acttggtggt
     2521 attggtaata ttttagatgg agatggttgg aagtagttgt tatactttca ttattgcaat
     2581 ttttttgaat taggcttttt tattctttaa agctgtttta taaattgcca tacatttttc
     2641 ataataagcc tcttctgaat aaaattcctc aaaagttgtt tttaattttt cttttaatgc
     2701 agaattatca ttttctataa ttgcaagcaa cttctttacc cagttagaaa tatcaaaatc
     2761 gttcataact aattcgccta agtggggttt aatttgctga actacttcgc cagtattata
     2821 agtaataaaa ggtatttgct gtgccatata ttctattaaa gccaatgggc cactttctgt
     2881 attagcagtg tgtatggcca tatcaaattt tggtaaaata gattgtacgt tgctacaatt
     2941 atgttcaatg gttactttat gctgtaaact attttcttca atttgattaa tgatagaagt
     3001 tagatattct gtataggcag gttgtcctaa tattgttaaa tgaaaatggc taggtaaaaa
     3061 tttcatcaac tcaatggcaa actcaatatt ttttggtggc ctaaaattgc ttaccattac
     3121 taaatgcttt ttatcggtat caattttttc tattttttct attggtagtt ttataataat
     3181 attttctaat aaaaatgtat tttctttttt aagttttaaa ttttctaaag cccattggta
     3241 taaactttta gatacagcaa taaaaacaac tttaggaaat attatttttt gatgccaatg
     3301 cactctttta tctacatcta tatttccaaa gtgttcatga aaaaatattt ttttacgtaa
     3361 accaaaaaaa gcagcagcaa caaaaagaaa acgtaggtta tgggcagagt gtacgtgaac
     3421 aatatcagca tgtttacacc attgcactaa ttgttgcata ctttttaagt gccatttatg
     3481 ctttctatta agttctttta tttctatacc ttcctgtaat tgatttgcaa taataccttt
     3541 tcttacagtg gtaataacag ttactttatg cccatgttta tgcagtaaat tacttaatgt
     3601 aatcagtact ttttctgctc cgccagcatc caatacatca ataatttgta caattttata
     3661 tgtattattt tcttgcactt tttagttacg aatgtacaca aaaacttaaa tgcacaaata
     3721 ggaatagttg agaattaatt tttttaaaaa taaaaaagtg aaagtattag ttacatagct
     3781 tgtttgatga tttccacaac aactttatca ataggcaagg ttaca
//
LOCUS       DAOQPK010000209         3783 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_16536_length_3783_cov_4.169796, whole genome shotgun sequence.
ACCESSION   DAOQPK010000209 DAOQPK010000000
VERSION     DAOQPK010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16536_length_3783_cov_4.169796"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..628
                     /locus_tag="PLU36_08975"
     CDS             77..628
                     /locus_tag="PLU36_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HMZ46921.1"
                     /translation="MKIANDIRMLSSGPRSGIGEVFIPDNEPGSSIMPGKVNPTQCEA
                     LTMIAAQVMGNDVAINVGGSMGHFELNVFKPVMIYNFLHSARLIGEGCVSFNDKCAIG
                     IEPLEANIKKHVDNSLMLVTALNTKIGYYNAAAIAQDAHKRGITLKQAATEYKLKGLD
                     FEHMTEQQFDEWVIPGNMCGEIK"
     gene            706..1803
                     /gene="corA"
                     /locus_tag="PLU36_08980"
     CDS             706..1803
                     /gene="corA"
                     /locus_tag="PLU36_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HMZ46922.1"
                     /translation="MSKNRYLKYFNLFALFDTKRTRDIFQINPTIPSQRVDAENIEID
                     LYCYNNNNVTLSHYNSVEQLNIQLSNDTITWINIDGIKKTEIEKICNQFKIHQLINED
                     ILSIGQRPKVDEIDEVMYCLLNMLYFNEQKGVVEPEQISIILGKNFLISIQEDPARDV
                     FNTLREKLKISNSKIRQSKADYLCYAMLDMIVDHYFLVMEKLGESIETLEEKIIRNSN
                     KRTLAEINSLRKELIVLKRNVVPVREIMNGFIRSENELLQERTTKYFKDIFDHINQAN
                     ELVENYRDMMMNLQDLYLSNVNLRMNEVMKVMAIVTCLLAPATVIGGIFGMNFEVIPI
                     AHQKWGFYLTVGIMLFIPALMLFIFKRRGWF"
     gene            complement(1857..>3783)
                     /locus_tag="PLU36_08985"
     CDS             complement(1857..>3783)
                     /locus_tag="PLU36_08985"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMZ46923.1"
                     /translation="IKPIDSFTLALRGADVLKNNWFFLFIDAMKDLNVQVFGFELLKN
                     FRFNTAKPSTKIYISSNTDWFDAKIEIQFGEQKVTVDDVKKALANKQQFVPLNDGTLG
                     ILPEEWIRKYSLLFKVGEGKTNNMKLSKYQFSVVEDLYNQRDEEELFIQLEEKYDTLR
                     YNHEIKKIKAPTHLKSILRPYQESGFQWLNYLSEVKWGGILADDMGLGKTIQALSFLQ
                     HLKEVNKSIKALVVCPTTLMFNWQNEIKKFTPALTYFIHHGGARLKSNLSNVAIDVII
                     TTYGTLRSDIKYFAEVEFDYVILDESQAIKNPASKVTKAACVLKAKNKLCLTGTPLQN
                     NTFDIFAQMNFLNPGMLGSQEFFKNEFAVPIDKLGEKEQKDHLRKLLYPFILRRTKEQ
                     VAKDLPEKQEMILYCEMGKEQRKIYDAYRNDFRDKILGVVQEKGIQKSQLTILQGLMK
                     LRQICDSPSILKEKQGEVFPNESVKLEELAREITENISNHKALIFSQFIGMLSLIKEN
                     MKANGIIYEYFDGTTSAAERERAINRFQNDDECRVFLISLKAGGVGLNLTAADYVYIV
                     DPWWNPAVEQQAIDRTHRIGQTKNIFAYRMICTDTVEDKIIHLQEKKRALAKDIITDD
                     EGFVKSLTKEDVEYLFS"
BASE COUNT         1310 a          565 c          567 g         1341 t
ORIGIN      
        1 ataagtttga agccttagca gcacatgatg ctattgttga agcccatggt gcattaaaac
       61 tgtagctgtt agtttaatga aaatagccaa tgatattaga atgttatcta gtggcccacg
      121 tagcggaatt ggagaagtat ttattccaga taatgagcca ggaagttcca ttatgccagg
      181 aaaagtaaac cccacccaat gcgaagcttt aacaatgatt gctgcacaag ttatgggcaa
      241 tgatgtagcc ataaatgttg gtggcagcat ggggcacttt gaactgaatg tttttaaacc
      301 cgtaatgatt tataattttt tacatagtgc aagattaatt ggcgaaggat gtgtaagctt
      361 taacgataaa tgtgcaatag gtattgaacc tttagaggcc aatataaaaa aacatgttga
      421 taacagctta atgttagtta ctgctttaaa tactaaaatt gggtattata atgctgctgc
      481 cattgcacaa gatgcacata aacgtggtat tactttaaaa caagctgcta ctgaatataa
      541 attaaaaggg cttgattttg aacacatgac agagcaacaa tttgatgagt gggttattcc
      601 aggaaatatg tgtggcgaaa taaagtaaaa aattacgtac ataattttca ttaaagtcgt
      661 tacttttata agtaccgact tttttattta taaattgatt tttttatgtc taaaaataga
      721 tacttaaaat atttcaactt attcgcttta ttcgatacta aacgaaccag agatattttt
      781 caaataaatc caactatccc ttctcaaaga gtggatgcag aaaatataga aattgatttg
      841 tactgctaca ataataataa tgttacccta tctcattata attctgtaga acaattaaat
      901 attcaactta gtaacgatac aataacatgg ataaatattg atgggataaa gaaaactgaa
      961 attgaaaaaa tttgtaatca gtttaaaata caccagttaa ttaatgaaga cattttaagc
     1021 attgggcaaa ggcccaaagt agatgaaatt gatgaagtaa tgtattgctt attaaatatg
     1081 ctttatttta atgaacaaaa aggagtagtt gaaccagagc aaataagtat tattttaggt
     1141 aaaaactttt taatctccat tcaagaagac cctgccagag atgtttttaa tacattaaga
     1201 gaaaaattaa aaataagcaa cagtaaaata aggcaaagta aggcagatta tttatgttat
     1261 gccatgttag atatgattgt tgatcattat tttttagtaa tggaaaaatt aggagaaagt
     1321 attgaaactc ttgaagaaaa aataataaga aatagtaata aaagaacttt agcagaaata
     1381 aacagcctta gaaaagagtt gattgtttta aaaagaaatg tagtgccagt aagagaaata
     1441 atgaatggat ttattagaag cgaaaatgaa ttattacaag aaagaacaac caaatatttt
     1501 aaagatattt ttgaccatat caaccaagct aatgaactag tggagaacta cagagatatg
     1561 atgatgaacc tacaggattt atatttaagt aatgtaaatt taagaatgaa tgaagtaatg
     1621 aaagtaatgg caattgttac atgtttactt gcaccggcaa ctgttattgg cggaattttt
     1681 gggatgaatt ttgaagtaat acctattgct catcaaaaat ggggttttta tttaactgta
     1741 ggaattatgc tatttatacc tgcattaatg ctttttattt ttaaaagaag ggggtggttt
     1801 taattaagaa tttgttaggt ataaaacgaa aaaattaaaa ttgatttctt aaaaaattag
     1861 ctaaataaat actctacatc ttctttggtt aatgatttta caaaaccttc atcatctgtt
     1921 ataatatctt tagccaaagc ccttttcttt tcttgtaaat gaataatttt atcttcaacg
     1981 gtatctgtac aaatcattct atatgcaaaa atatttttcg tttgtcctat tctgtgtgtt
     2041 ctatcaatag cttgctgttc tacagcaggg ttccaccaag gatcaacaat atatacataa
     2101 tctgcagcag ttaaatttaa accaacacca ccagctttta gtgaaattaa aaacactcta
     2161 cattcgtcat cgttttgaaa acgattaatt gccctttctc tttctgcagc actagtagtt
     2221 ccatcaaaat attcatagat aattccattg gctttcatat tctctttaat tagagacaac
     2281 attcctataa actgagaaaa aataagagct ttatggttac ttatgttttc agtaatctct
     2341 cttgcaagct cttctaattt taccgattca ttaggaaaaa cttctccttg tttttctttt
     2401 aatatactag ggctatcgca aatttgcctt aacttcatta agccttgcaa aatagtgagc
     2461 tggctttttt gtattccttt ttcttgaaca acccctaaaa ttttatctct aaaatcattt
     2521 cggtaagcat cataaatttt tctttgctct ttacccatct cacaatacaa aatcatttct
     2581 tgtttttcgg gcaaatcttt tgctacttgt tcttttgttc gtcttaaaat aaaagggtac
     2641 aataattttc ttaaatggtc tttttgctct ttttctccta atttatcaat aggtactgca
     2701 aattcatttt taaaaaattc ctggcttcct agcatgcctg ggtttaaaaa attcatttgt
     2761 gcaaaaatat caaatgtgtt gttttgcaaa ggggtacccg ttaggcataa cttatttttt
     2821 gcttttaaaa cacatgctgc tttggttact ttactagcag gatttttaat tgcttggctt
     2881 tcatctaata ttacatagtc aaattcaact tctgcaaaat attttatatc acttcttaaa
     2941 gtaccataag tggtaataat aacatctata gcaacattag ataaattaga ttttaatctg
     3001 gctccgccat gatgaataaa ataagtaagt gcaggtgtaa atttttttat ttcgttttgc
     3061 cagttaaaca ttagtgtagt agggcaaaca accaatgctt ttatagactt atttacttct
     3121 tttaaatgtt gtaaaaaact tagagcttga atggttttac ccaagcccat atcatcagct
     3181 aaaattccac cccattttac ttcgcttaaa taatttaacc actgaaaacc actttcttga
     3241 tatggcctta atattgattt tagatgagta ggagctttaa ttttttttat ttcgtggtta
     3301 tatctaagtg tatcgtattt ttcttctaac tgtataaata attcttcttc gtctcgttga
     3361 ttgtataaat cttcaacaac gctaaattga tatttactca atttcatgtt gttggtttta
     3421 ccttcgccaa ctttaaatag taaagagtat tttcttatcc attcttctgg taaaatacct
     3481 aaagtgccat cattaagtgg tacaaattgt tgcttgttgg ccaaagcttt tttaacatca
     3541 tcaacagtta ctttttgttc accaaattgt atttcaattt ttgcatcaaa ccaatctgta
     3601 ttgctgctta tataaatttt ggtagatggc tttgcagtat taaacctaaa atttttaagc
     3661 aactcaaacc caaatacctg cacatttaaa tccttcattg catctataaa caaaaagaac
     3721 caattatttt ttaaaacatc tgcaccacgc aatgcaaggg taaaactatc aataggtttt
     3781 ata
//
LOCUS       DAOQPK010000210         3773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_16619_length_3773_cov_4.029048, whole genome shotgun sequence.
ACCESSION   DAOQPK010000210 DAOQPK010000000
VERSION     DAOQPK010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16619_length_3773_cov_4.029048"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="PLU36_08990"
     CDS             complement(<1..668)
                     /locus_tag="PLU36_08990"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HMZ46924.1"
                     /translation="MLKSTLLKAVEDGAKILKEYFDKQFVISNKEGINNLVTEVDHKS
                     EQAIINCIKNSFPNHFILSEEVGEIVQDSEYKWIIDPIDGTVNYANGIPICCVSIGIE
                     KKDEMIMGAVYNPFMNEFYFAEKNNGAFLNNHKIKVSHKTDILSSCLVTGFPYTYLDA
                     TNGPLQIFEKFIRKGIPVRRLGSAAIDLCWVAAGRFDGFYEHKLSAWDSAAGFIIVEE
                     AGGKV"
     gene            complement(712..2115)
                     /locus_tag="PLU36_08995"
     CDS             complement(712..2115)
                     /locus_tag="PLU36_08995"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ46925.1"
                     /translation="MKLSFHGAAQTVTGSKHLITLKNGKKILLDCGLFQGLGKETGSF
                     NASFGFNAEEVDVMILSHAHIDHSGLIPKLYKEGFRGKIFCTGATFDLTKILLEDSAT
                     IQRDDTRFLNKRRAKSGLPPIEPLYTIEDAEQSFSLLEKQAYQHWFKVTDGVQAMFTD
                     AGHIIGSSSVHLKINEEGKNIRISFSGDVGRYRDLILKSPEVFPQADYIIIESTYGDK
                     LHEDAFNSIDTLLNWVNKTCVEKKGKLIIPAFSVGRTQELLYMLNQLSLENRLPKIPV
                     YVDSPLSIEATEVVKSYPRYFNNRVKKVLDIDEDPFDFPGLNYIKSADESKALNANKQ
                     AMIIISASGMADAGRIKHHIANNIDNNKNTILLVGYCEPHSLGGRLLNGSKEVRIYGE
                     MHHVIAEVGIMRTMSAHGDYDDLSQYLSCQNPALVKSIFVVHGEIDVMHEFKRRLNKK
                     GFKEVEIPKMHESFQLD"
     gene            complement(2164..3171)
                     /locus_tag="PLU36_09000"
     CDS             complement(2164..3171)
                     /locus_tag="PLU36_09000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ46926.1"
                     /translation="MLPSVAIVILNYNGINYLQQFLPSVLSSTYSNKKIIVADNASTD
                     DSVNWVKENYPQITVLINTQNNGYAGGYNWCLKSVEADYFVLLNSDVEVTANWIEPII
                     ELMQADTKIAACQPKILSWHHKEKFEYAGACGGFIDLYGYPFCRGRIFDFCEDDKGQY
                     NSVEKIFWASGAALFIKSKVFVDLNGFDENFFAHMEEIDLCWRLQLSGYTIAVQPASI
                     VYHVGGGTLPKSYQKTFLNFRNNWIMLCKNLTIKEKFLIFPVRFFLDCTTACKALLSK
                     DTEMARAIFNAHIAFIKWKFSSKYKSVQPNKPLYKLNGVLLNSIVFKYFVRKKKRYSD
                     L"
     gene            3459..>3773
                     /locus_tag="PLU36_09005"
     CDS             3459..>3773
                     /locus_tag="PLU36_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HMZ46927.1"
                     /translation="MNIYVGNLNWNMTSEDLQELFAPYGEVASAKIVTDKFKNNRSKG
                     FGFVEMNDDDAARAAISALNDTDVQDRKIVVNESTPRPEGERRSSGGGGGFRKSGGFG
                     GGN"
BASE COUNT         1303 a          673 c          488 g         1309 t
ORIGIN      
        1 actttaccgc ctgcttcttc tacaataata aacccagcag cactatccca tgcactaagc
       61 ttatgttcat aaaaaccatc aaatctaccg gcagcaaccc aacataaatc aattgctgca
      121 ctgcctaatc ttctaacagg aatacctttt ctaataaact tttcaaatat ttgcaaaggg
      181 ccgtttgttg catctaaata agtataagga aagcctgtaa ctaaacaact acttaaaata
      241 tctgttttat gagatacctt aattttatga ttatttaaaa aagctccatt atttttttca
      301 gcaaaataaa attcgttcat aaatgggtta tacacagcac ccataatcat ttcatccttt
      361 ttttcaatac caatactaac acaacaaatg ggtattccat tagcataatt tacagtacca
      421 tcaatggggt caattatcca tttatattca ctatcttgta ctatttcacc aacctcttca
      481 cttaaaataa aatgattagg aaaactattt ttaatacaat taataatagc ttgctcactt
      541 ttgtgatcta cttctgttac caggttatta ataccttctt tattagaaat tacaaactgc
      601 ttatcaaaat attcttttaa tatttttgca ccatcttcaa cagcttttaa tagtgtactt
      661 tttagcatag gcaaatataa attacaatta aatgttactg aaagtttcta actaatctaa
      721 ttgaaaactt tcgtgcattt taggaatctc tacttcttta aatccttttt tattaagcct
      781 tcttttaaat tcatgcataa catctatctc tccatgtacc acaaaaatgc ttttaactaa
      841 agcaggattt tgacaagata agtattggct taaatcgtca taatcgccat gtgcactcat
      901 ggttctcata atgccaactt ctgcaattac atgatgcatt tcgccataaa ttctaacctc
      961 ttttgatcca tttaatagtc ttcctcctaa actatgaggc tcgcagtagc caactaataa
     1021 aatggtattt ttattattgt ctatattgtt agcaatatga tgttttattc taccggcatc
     1081 agccattcca cttgcactaa taattatcat tgcttgctta ttggcattta gtgctttact
     1141 ttcatcggca ctttttatat agtttaatcc aggaaaatca aaagggtctt catcaatatc
     1201 taacactttc ttcactctat tattaaaata tcttggataa ctttttacta cttcagtagc
     1261 ttctatactt aatgggctat ccacataaac aggaatttta ggcaacctat tttctaaact
     1321 taattggttt agcatataaa gtaattcttg tgttctgcca acgctaaaag ccggaataat
     1381 cagtttgcct tttttctcaa cacaagtttt gttaacccaa ttcaacaaag tatctatact
     1441 attaaaagca tcttcatgta atttgtcgcc ataagtactt tcaataataa tataatctgc
     1501 ctgaggaaag acttcaggac tttttaaaat taaatctctg taacgaccaa catctccact
     1561 aaaacttatt ctaatatttt ttccttcctc gttaattttt aaatgaacag aagaactacc
     1621 aataatatga ccagcatcgg taaacatggc ttgtacccca tctgttactt taaaccaatg
     1681 ttgatatgct tgtttttcta gcaatgaaaa ggattgttct gcatcttcaa ttgtgtaaag
     1741 aggctcaata ggtggcaacc cactcttggc tcttctttta tttaaaaacc ttgtatcatc
     1801 tctttgtatt gtagctgaat cttctaataa tattttagtt aaatcgaaag tagctcctgt
     1861 acaaaatatc ttccctctaa aaccttcttt atacaatttt ggaataaggc cactatggtc
     1921 aatatgggca tgagaaagaa tcatcacatc tacttcctcc gcattaaacc caaaacttgc
     1981 attaaatgaa cctgtctctt tccctaagcc ttgaaacaag ccacaatcta ataaaatttt
     2041 tttaccattt tttaaagtaa ttaaatgttt agaaccagta acagtttgtg cagcaccatg
     2101 aaaagataat ttcatttttt aaaatttatg aattaacgaa tactagagtt taaaatttct
     2161 tagctacaaa tcagaataac gctttttttt cctaacaaaa tatttaaata ctatagaatt
     2221 taataaaact ccatttagtt tataaagtgg tttattaggt tgtacacttt tatattttga
     2281 agaaaacttc cattttataa aagcaatatg agcattaaaa atggctctag ccatttctgt
     2341 atctttactt aataaagctt tacaagcagt agtacaatct aaaaaaaatc taactgggaa
     2401 aattaaaaat ttttctttaa tagttaaatt cttacaaagc attatccaat tatttctaaa
     2461 attaagaaaa gttttctgat aacttttggg taatgttcca ccacctacat gataaacaat
     2521 agatgcaggc tgaacagcaa tagtataacc agataattgt aatctccagc aaagatcaat
     2581 ttcttccatg tgagcaaaaa agttttcatc aaatccattt aaatccacaa aaactttgct
     2641 cttaataaac aatgctgcac cagatgccca aaatattttc tcaacagaat tatattgccc
     2701 tttatcatct tcacaaaaat caaaaattct tcctctacaa aacgggtagc catataaatc
     2761 tataaagcca ccacaagcac cagcatattc aaatttttct ttgtgatgcc aggataaaat
     2821 ttttggttgg caagccgcaa ttttagtatc tgcttgcatt aattctatta ttggctctat
     2881 ccaattagca gtaacttcaa catcagaatt taagagtaca aaataatctg cctcaacact
     2941 tttaagacac cagttgtatc caccagcata accattattt tgagtgttta ttagaactgt
     3001 aatttgaggg taattttcct tcacccagtt aacactatca tctgtagagg cattatctgc
     3061 tacaataatt tttttattag aataagttga tgaaagaaca gaaggaagaa actgttgtaa
     3121 ataatttatt ccattatagt ttaatataac aattgcaaca cttggtaaca aaaaaaatag
     3181 tttttgtaaa aataagggtt gtaaaagttt taattagaaa ttaatgaaaa tgtaacactg
     3241 cattacgtaa tacaatttta aatattaacc ttaaaatgta taactgaaac tatctactaa
     3301 gaaaagattg aaaaaaaact tgcttaatta gataaaagta ctattttcgt gttttccaat
     3361 gaccggtttt ccatatttcc ggaaggtttt aacgtaaatg cttcaggttt tttggttact
     3421 gtttttattg gttttattta aatttataaa ttcaagaaat gaacatttac gttggtaatt
     3481 taaactggaa tatgaccagt gaagatttac aagaattatt tgcaccttat ggtgaagtag
     3541 catctgcaaa aattgttaca gacaaattca aaaacaatcg cagcaaaggc tttggctttg
     3601 ttgaaatgaa cgatgatgat gcagcacgtg cagcaatcag tgcattaaat gatactgatg
     3661 tgcaagacag aaaaattgta gttaatgaat caactccacg tcctgaaggt gaacgtagaa
     3721 gcagtggcgg tggcggcggc ttcagaaaaa gtggtggctt tggtggaggc aac
//
LOCUS       DAOQPK010000211         3765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_16679_length_3765_cov_4.369542, whole genome shotgun sequence.
ACCESSION   DAOQPK010000211 DAOQPK010000000
VERSION     DAOQPK010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16679_length_3765_cov_4.369542"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="PLU36_09010"
     CDS             complement(<1..610)
                     /locus_tag="PLU36_09010"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMZ46928.1"
                     /translation="MKKILFAFICLMLIQVVHAQNSTSLKGKVTDTLSKQNLKDASIS
                     MLNAKDSTVESFGLAKTDGTFEIKGFAMGSYLLQISFNGYESYFKSVVFNKQNPTLDL
                     GTIILKPQAKELEGVSVVAAPIQVKGDTTEFNAGSFKTKPNATAEDLLKKLPGVQVEK
                     DGSVKAQGQTVKRVLVDGKRFFGDDPTTATRNLPTDVIDKVQV"
     gene            855..1250
                     /gene="mscL"
                     /locus_tag="PLU36_09015"
     CDS             855..1250
                     /gene="mscL"
                     /locus_tag="PLU36_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HMZ46929.1"
                     /translation="MMKEFKEFAMKGSLIDLAIGFVMGAAFTKVTGAFINGMVMPLVG
                     MIQGKDLSDWKFVLKDADKVAGTEEVAIKYGSFISVTIEFIIVAFVMFMVIKAINRMK
                     KKQEEAPAAPAEPSSTDKLLIEIRDALKK"
     gene            complement(1309..3546)
                     /locus_tag="PLU36_09020"
     CDS             complement(1309..3546)
                     /locus_tag="PLU36_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46930.1"
                     /translation="MKKYFVGFWYSMPVQLLFLHFRKYQIFLVLWYVLFATVGGIFLN
                     NFGASSLFLAPEYFNEVSFSSTAIVGFSIGVFIMSWNITTFILHGKHIQFLASTAQPF
                     LKYCINNAVLPILFLIFYFFSALQYNNQKELKPIVEIALLSSGFLIGFFLSITISFMY
                     FFSADKRIYKNMATVIHEANKNYAIAFTENPLPKEKSQIRIDWFFSARMHLRKPRDIR
                     HYSTDFLDRIFKQHHLAAILAFFLAFIALIALGFSSDSRVFQIPAAASVTIFFALIIA
                     FGGAFTLFLKSWSIPIIILLYVLLNSLYKYNILDLRNKAYGLNYENKNNRPEYSKESI
                     YALASKENIENDKLTFLKRLLHWKANQTEDLPILYIINTSGGGVRSSTFTFSVLQMLD
                     SVVFNGNLMKKTFFINGASGGMLGAAYYRELYFRKQQGFNINLQNKQYLDDISKDLLN
                     PIFSSFVSRDIIGPVQKFKKFGYEYVKDRGYAFERSLNVNTRGYLNKRLIDYAEVENK
                     AIIPTMFFNSTISTDGRKFVMCSQPVRFLMNHVNANDLSSEPAPDIIDFNSFFSKQNS
                     NNIGILSALRMNATFPYVLPNVWLPTNPVIDVMDAGLRDNFGQENTLRFIEVYKNWLK
                     ENTSKVVIIQIRDRSINDWDEVEKNKSLWSAFTKPAFLLQYNWYRLQDYYQHAQLEYM
                     NESYGSNLQQVSFQYVPSNKSKAASLSFHLTTNEKKEILNSLHNKVNVDELTKLKNFL
                     VEKED"
BASE COUNT         1298 a          547 c          556 g         1364 t
ORIGIN      
        1 aaacctgtac tttatcaatt acatcggtgg gtaaatttct tgttgcagta gtagggtcat
       61 cgccaaaaaa tcttttccca tctactaaaa cacgttttac tgtttgtcct tgtgctttaa
      121 cagagccatc tttttctact tgtacaccag gtaatttttt taataaatct tctgctgtag
      181 cattgggttt tgttttaaaa cttcctgcat taaattctgt tgtatctcct ttaacttgta
      241 taggagcagc aacaaccgaa acaccctcta actcttttgc ttgtggtttt aagataatag
      301 tacctaaatc taaggttgga ttttgtttat taaaaacaac tgatttaaaa taggattcat
      361 aaccattaaa acttatttgt aaaaggtaag agcccatagc aaagcctttt atttcaaagg
      421 ttccatctgt tttggcaagg ccaaaagatt caactgtgga atcttttgcg tttaacatgc
      481 ttatagatgc atcttttaaa ttttgttttg aaagtgtatc tgttactttt cctttaaggc
      541 tagtactatt ttgagcatga actacttgaa ttaacattaa acaaatgaaa gcaaataaaa
      601 tttttttcat aaatgggaga gttgattggg aataatctta tgtacgaaaa taatcgttta
      661 actgttgtat taacgttaaa taatataaca atggtaaaga aataatatga atggtaatta
      721 gttgtaatta gggtaagaat ttttaataaa aactagagat gatttttttt gaaaataaat
      781 ttctttctaa ccatttttcc tagcttaaac ctaaatttga cataattatt aactaaaact
      841 aactaattat gggaatgatg aaagaattta aggagtttgc catgaagggc agccttatag
      901 atttagcaat aggctttgta atgggtgctg cttttacaaa agttacagga gcttttatta
      961 atggtatggt gatgccttta gtaggtatga tacaaggtaa agacttatct gactggaagt
     1021 ttgttttaaa agatgcagat aaagtagcag gtacagaaga agtagctatt aagtatggtt
     1081 cttttatttc ggtaactatt gagtttatta ttgttgcttt tgtaatgttt atggtaatta
     1141 aagctattaa cagaatgaag aaaaaacaag aggaggcacc tgcagctcca gcagaacctt
     1201 catctaccga caagctttta attgaaatta gagatgcatt gaaaaagtag catttattta
     1261 ttgcttacat tttttaatac ccttttttta agggtatttt ttttgtaatt aatcttcttt
     1321 ttctactaaa aaatttttaa gcttggttaa ttcatccaca tttactttat tatgaagaga
     1381 gtttaagatt tctttttttt cgttagtagt taaatgaaaa cttaagcttg cagctttgct
     1441 tttgttagat ggaacatatt gaaagcttac ttgttgtaaa ttactgccat aactttcatt
     1501 catatactct aactgggcat gttggtaata atcttgcagt ctataccagt tatattgcaa
     1561 taagaatgca ggcttggtaa atgcactcca taagctttta tttttctcca cctcatccca
     1621 gtcgtttata cttctatctc ttatttgaat gataactact ttagaagtat tttcttttaa
     1681 ccaattttta taaacttcta taaaacgcaa ggtgttttct tgtccaaaat tatctcttaa
     1741 tcctgcatcc ataacatcaa taacaggatt ggttggcagc caaacattgg gtaaaacgta
     1801 aggaaaagta gcattcattc ttaatgcact taaaatacca atattgttgc tattttgctt
     1861 gctaaaaaag ctattaaaat caataatatc tggtgcaggt tcagaagata aatcatttgc
     1921 attaacatga ttcattaaaa acctaacagg ttgggagcac attacaaatt ttctaccatc
     1981 ggtgctaata gtactattaa aaaacatggt aggtataatt gctttgtttt ccacttcggc
     2041 ataatctatt aatcttttat ttaaataacc tcttgtatta acgtttaaag atctttcaaa
     2101 agcatagcct ctatctttca catattcata tccaaatttt ttaaattttt gaacaggtcc
     2161 aataatatct ctactaacaa aagaagagaa tatggggttt agtaaatctt tagaaatatc
     2221 atctaaatat tgtttgtttt gaagatttat attaaagcct tgttgcttac gaaaatacaa
     2281 ttctctataa taagcagcac ctaacatacc accagatgca ccattgataa aaaaagtttt
     2341 tttcattaaa tttccgttga atacaacaga gtctaacatt tgcaacacac taaatgtaaa
     2401 agtagaactt ctaactccgc caccacttgt attaatgatg tataatatgg gtaggtcttc
     2461 tgtttgattg gctttccagt gtaataatct ttttaaaaaa gttagtttgt cattctctat
     2521 attttctttg cttgctaaag cataaatact ttctttacta tattcaggtc ggttgttttt
     2581 attttcataa ttcaaaccat aagctttgtt tcttaagtct aaaatattgt acttgtacaa
     2641 actatttaat aaaacatata aaagaataat tattggaata ctccaggatt taagaaacaa
     2701 tgtaaatgct ccaccaaaag caatgattag agcaaaaaaa atggttacac tagctgcagc
     2761 aggaatttga aataccctag agtctgacga aaaacctaat gctattaatg ctataaaagc
     2821 taagaaaaat gcaagtattg cagctaaatg gtgttgctta aaaattctat ctaaaaaatc
     2881 ggttgagtaa tgccttatat ctcttggttt gcgtaagtgc attctggcac taaaaaacca
     2941 atcaattctt atttgacttt tttcttttgg taatgggttt tctgtaaatg caatagcata
     3001 gtttttattg gcttcgtgaa ttactgtagc catgttctta tatattcttt tatcggcact
     3061 aaaaaaatac ataaatgata tagtgatgga taaaaagaat cctattaaaa aaccactgct
     3121 tagtaaagct atttctacaa ttggttttag ctctttttga ttattatact gtaaggcact
     3181 aaaaaaataa aatattaaaa ataaaattgg taatactgca ttgttgatac aatattttag
     3241 aaatggttga gctgtagaag caagaaactg tatgtgtttg ccatgtaaaa taaaagttgt
     3301 gatattccaa ctcattataa aaacaccaat agaaaagcct actatggctg tactggaaaa
     3361 actaacctcg ttaaaatatt ctggagcaag aaatagtgag ctagcaccga agttgtttaa
     3421 aaaaattccg ccaacagttg caaataaaac ataccataaa actaaaaata tttgatattt
     3481 tctaaaatgc agaaacagta attgtacagg catactgtac caaaaaccta caaaatattt
     3541 tttcattcta aaatgtattt accaaactaa tattaattaa ccctcactca tttttgtaat
     3601 aattatttct tttaaagata ctgaattttt attttgaatt gcctattgtt aatattttat
     3661 tttatacctg cctaatgagg tttttgttga aagtaaacta ttgaaagaac agagtataaa
     3721 gtgtattatt gcaatatgct gcaaccatat tatcaggaaa atata
//
LOCUS       DAOQPK010000212         3722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_17011_length_3722_cov_4.835015, whole genome shotgun sequence.
ACCESSION   DAOQPK010000212 DAOQPK010000000
VERSION     DAOQPK010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17011_length_3722_cov_4.835015"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PLU36_09025"
     CDS             complement(<1..683)
                     /locus_tag="PLU36_09025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ46931.1"
                     /translation="MIKVFLKRKIANRIANGHPWIFANEIDRVEGDIVQGAIVEVYYA
                     DNKYCGSGYINPKSQIMVRLLTRKKEQIDEAFFYNKILQSWNYRKQIGYTENCRLIFG
                     EADELPALIIDKFNDYFVIQTLSYGMEVWKPAITKALQQIFNPKGIYERNDVPVRELE
                     GLTQQKGFLSEPFNTNIIINENGLKFNVDIENGQKTGYFLDQQDNRRAIQHIVKNADV
                     LGAFTYTGT"
     gene            745..1737
                     /locus_tag="PLU36_09030"
     CDS             745..1737
                     /locus_tag="PLU36_09030"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 isoprenoid biosynthesis enzyme"
                     /protein_id="HMZ46932.1"
                     /translation="MNYFQLCFYATDYGLSAYKQLNFIKAFLKPMVEKHCAALDYPLS
                     KKEQKKVGFYYPLFNHIVNCENYLCIKGRKLTDEETKRMAIISAMATLHDDLIDEENW
                     EKEKYFKVLNRTLPNELHTPKVKLIFALEDELHKIWTPTEKYKEALKTATEWQVVSSK
                     QLNPNISLEDVLYISENKCGNSSLLWASVMNEEWLPEDDAFIFQSGFVGQIVNDLFDA
                     FKDREDGVYTIVRKTETVNQAKEIFIRECAKLHQLILQCNVSASNKLVTIRRMACIHA
                     FALVAINHLQQTENKYGKPINWAKPLRKELVTDMAFWSNKFKLLQYACSLAELR"
     gene            complement(1742..2938)
                     /locus_tag="PLU36_09035"
     CDS             complement(1742..2938)
                     /locus_tag="PLU36_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HMZ46933.1"
                     /translation="MVQISKRGEAMPASPIRKLVPYAEAAKKKGRTVYHLNIGQPDIE
                     TPKQVRDAIHQSDFKILEYTHSAGLESYRIKLSQYYKSVGIDVNHNEIIITTGGSEAI
                     LFGFMACLDPGDEVIIPEPFYANYNGFAIAAGINVVPITSYITNGFALPPIEEFEKKI
                     TAKTKAIVICNPNNPTGYNYSENEMQELKEIILKHNLYLFADEAYREFTYEEKQHSAM
                     HLKGVENNVVLMDTISKRYSACGARIGAFVTKNKQLIDATMKFAQMRLCSPYLEQLAG
                     EAAIDLPPDYFNITKAEYKTRRDLIVKRLNAIEGVVCPNPGGAFYAMAKLPIDDSDKF
                     CQWLLESFEYNNQTVMLAPATGFYGTQGLGKDEVRLAYVLNVGAINKAMDCLEQALKV
                     YPGKTI"
     gene            3098..>3722
                     /locus_tag="PLU36_09040"
     CDS             3098..>3722
                     /locus_tag="PLU36_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46934.1"
                     /translation="MSLKFVFTLLFFTISSALIAQKKTDTSYFMNNYRQGDKYFRENP
                     DSAIFYYKKAEEWLLKNSWFDSSIDATTMLSNMGRTYRVVGNPLSSQQTLQKALNNAR
                     KYNHNSEFRLIFLRLTFLHKEIINKNWAFNYPPVNETKTEEVYFPISKIEPLGKDSLE
                     ITVAAGKLDGIINDSQTCRINSRLIEGDTLEHKSVKTFVSVNFTKVEN"
BASE COUNT         1268 a          577 c          548 g         1329 t
ORIGIN      
        1 aatgttcctg tataagtaaa agcccctaat acatcggcat tttttacaat atgctgtatg
       61 gctcttctat tatcttgttg atctaaaaaa tagcctgttt tttgaccatt ttcaatatct
      121 acattaaatt ttaaaccatt ttcatttata ataatattgg tattaaaagg ttcggataaa
      181 aagccttttt gttgtgttaa accttctaat tctcttactg gtacatcgtt tctttcataa
      241 atacctttag gattaaaaat ttgttgaaga gcttttgtaa ttgcaggttt ccaaacttcc
      301 attccataag atagtgtttg aataacaaaa taatcattaa acttatcaat gataagtgct
      361 ggcaattcat ctgcttcgcc aaaaattaat ctgcagtttt ctgtataacc aatttgcttt
      421 ctatagttcc agctttgcaa aattttattg taaaaaaaag cctcatctat ttgttccttt
      481 tttctagtaa gtaaccttac cataatttga gatttagggt taatatatcc actaccacag
      541 tatttattat ctgcataata cacttcaaca atagcacctt gaacaatgtc tccttctacc
      601 ctatcaattt catttgcaaa aatccaagga tgaccgttag caattctgtt tgctatcttt
      661 ctttttagaa aaacttttat catttggcaa atatagtttt atgcaatgtt atgcgtatgt
      721 agttaaatca attatattgc aaaggtgaat tattttcaac tctgttttta tgctaccgat
      781 tatggtttaa gtgcctataa gcaattaaat tttattaagg cttttttaaa gcctatggta
      841 gaaaagcatt gtgcagcttt agattatcct ttatctaaaa aggagcaaaa aaaagttggc
      901 ttttattatc ctttatttaa tcatattgta aactgcgaaa attatttgtg tataaaaggc
      961 agaaagttaa cagatgaaga aaccaaaaga atggctatta tttctgcaat ggctacctta
     1021 catgatgatt taattgatga agaaaattgg gagaaagaaa aatattttaa agtgttaaac
     1081 agaactttac ccaatgaatt acacacacca aaagtgaaat taatttttgc tctagaagat
     1141 gaacttcata aaatttggac accaacagaa aaatataaag aagctctaaa aactgctaca
     1201 gaatggcagg ttgtttcttc taaacagtta aatcctaata ttagcttgga ggatgtgtta
     1261 tacatttctg aaaataaatg tggtaacagt tcattattat gggcaagtgt tatgaatgaa
     1321 gagtggttgc cagaagatga tgcttttatt tttcaaagtg gttttgtggg gcaaattgtg
     1381 aacgatttat ttgacgcatt taaagataga gaagatggtg tttataccat tgtaagaaaa
     1441 acagaaacag tgaatcaagc aaaagaaata tttataagag aatgtgccaa attacatcag
     1501 ctaattttac aatgtaatgt atctgcaagt aataaattag taactattag aagaatggct
     1561 tgtattcatg catttgcatt ggtagccata aatcatttac aacaaacaga aaataaatat
     1621 ggtaaaccta ttaactgggc taaaccatta agaaaagaat tagtaaccga tatggctttt
     1681 tggagcaata aatttaagct actacaatat gcttgttctt tagctgaatt aagataacaa
     1741 cttatattgt tttaccagga tatactttta atgcttgttc taaacaatcc atagctttat
     1801 taatagcacc tacatttaaa acataagcta atctaacttc atctttacct aagccttgtg
     1861 tgccataaaa gcctgtagct ggggcaagca taacagtttg gttattgtat tcaaaacttt
     1921 ctaacagcca ttggcaaaac ttatcactat catcaatagg taatttagcc attgcataaa
     1981 atgcaccacc agggttaggg catacaacac cttcaattgc attcaatctt tttacaatta
     2041 aatctcttct tgttttatat tctgctttgg taatattaaa ataatcagga ggtaaatcaa
     2101 ttgctgcttc tcctgctaat tgctcaagat aagggctaca caaacgcatt tgtgcaaact
     2161 tcatagtagc atctattaat tgcttgtttt ttgtaacaaa tgcaccaatt cttgcaccac
     2221 aagcactata ccttttacta atggtatcca ttaaaacaac attattttct acacctttta
     2281 agtgcatggc actatgttgt ttttcttcgt aggtaaattc tctataggct tcatcggcaa
     2341 ataaatacaa gttgtgtttt aagataattt cttttagttc ttgcatttca ttttcgctgt
     2401 aattgtaacc agttggatta ttaggattac aaattacaat agcctttgtt ttggcagtta
     2461 tttttttctc aaactcttct attggtggta atgcaaaacc attggtaata taagatgtta
     2521 ttggcaccac atttatacct gcagcaatgg caaagccatt atagtttgca taaaaaggtt
     2581 ctggaataat tacttcatcg cctgggtcta aacatgccat aaagccaaat aatattgctt
     2641 cacttccacc agttgtaata attatttcgt tgtggtttac atcaattcct acacttttat
     2701 aatattgaga taatttaatt ctatagcttt ctaaacctgc actatgtgta tattctaata
     2761 tcttaaaatc tgattggtgt atagcatctc ttacttgttt tggagtttca atatcgggtt
     2821 gccctatgtt taaatgataa actgttcttc ctttcttttt tgctgcctca gcataaggaa
     2881 ctagttttct aattggcgaa gcaggcattg cctctcctcg tttacttatt tgtaccatgt
     2941 ggcaaatata agtagtaaat ttttcaagtt ttatttaatc atattctttt atcattttaa
     3001 gagagatatt taaaaataac ctaatggtaa agaatgtaaa taagtgctgt tgggtatttc
     3061 tataatatta atttcattta cctttaaaat aaaaaaaatg tctttaaagt ttgtatttac
     3121 tctactgttt tttactattt cttcggcact aattgctcaa aaaaaaacag acacttccta
     3181 tttcatgaac aattatagac agggtgataa atattttaga gaaaatccag atagtgcaat
     3241 attttactac aaaaaggccg aagaatggct tttaaaaaat tcttggtttg atagctccat
     3301 tgatgcaacc acaatgttat ctaatatggg ccgtacttac agagttgtag gcaacccttt
     3361 atcttctcag caaacattac agaaagcctt aaacaatgca agaaaatata atcacaactc
     3421 tgaatttaga ttaatttttt tgaggcttac ttttttacat aaagaaatta tcaataaaaa
     3481 ttgggctttc aactatccac cagttaatga aacaaaaaca gaagaagttt atttccctat
     3541 aagtaaaata gaacctttag gtaaagattc tttagagatt acagtagctg caggtaaatt
     3601 agatggtatt ataaacgact ctcaaacttg tagaataaat tctagactta ttgaaggaga
     3661 tactttagag cacaaatcag taaaaacatt tgtaagtgta aactttacta aagtagagaa
     3721 ta
//
LOCUS       DAOQPK010000213         3691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_17276_length_3691_cov_4.948295, whole genome shotgun sequence.
ACCESSION   DAOQPK010000213 DAOQPK010000000
VERSION     DAOQPK010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17276_length_3691_cov_4.948295"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PLU36_09045"
     CDS             <1..560
                     /locus_tag="PLU36_09045"
                     /inference="COORDINATES: protein motif:HMM:NF023433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46935.1"
                     /translation="RDWLRREKSGKETEESTIDWDDVVFEVDLLKSQEINLDYILELI
                     FEHNKKTKDKDTLITEIRRVIRASVGNRAKESLVVDFINETDLDTIQDKANVIDSFFV
                     FAQNKQKTEALELITEENLNVEEAKRYITTSLKREFASENGTELNALLPKMSPLNPQY
                     LTKKQSVFQKLVSFVEKFKGVGGQL"
     gene            876..2306
                     /locus_tag="PLU36_09050"
     CDS             876..2306
                     /locus_tag="PLU36_09050"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HMZ46936.1"
                     /translation="MKYLSICLFILSNIFFTHVFAQNSTSNFKKLNIINTTNDSGFLT
                     IGSININGNKRTKDYIILREVPFKLGNKYKPHELTGLLELARNQIMNTALFVDAQVYV
                     TNLLKDTAFINIDVKERWYIFPIPYFNLVDRNFNQWINEHKASLDRVNYGIKFFHFNV
                     SGRNDKLSLDVIGGYSQQLQFNYTNPFADKTLKHGFSVGFGYVRQKELNYASLNNKQV
                     FVRTNSFVRDGFRSLLTYSYRPDSKYRFYVSLGLNMDKISDTVLKLNPTYFPYYTNKI
                     TYPTLLVMLQYFGVDYIPFPKKGFLYQVSMFNRSFNKKANLFQLSVKTTNVLPLATNS
                     FLNIHTNSILKIGDNRSYFNQSLMGYNEVFLRGLESYVIDGMAGVVANNSLYQKLFKF
                     VFKNPIKIKGHENIPFHFYAKLFSDFGYAYNKYAAVNNPLANKFLYTAGAGIDITTIY
                     DFVFRIEYGLNQLGNTAFGLRVKGEF"
     gene            complement(2307..3380)
                     /locus_tag="PLU36_09055"
     CDS             complement(2307..3380)
                     /locus_tag="PLU36_09055"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMZ46937.1"
                     /translation="MRLFVFSFLSILILSNVAFTQPNKQDDNLFAAIKEKDVKKFHKA
                     IKKGANINARNSQNFTPLHAIFEYNNYGLGSAENEMFETLIQLGADVNAEINSKYHNT
                     PLVSAIRANACKYIEPLIKAGANVKVGRLLHLACFKTSYDCVEILVKNGLDVNAVDDL
                     NETPLIALLTNNDPYNKTIKILEYLHKKGADIFILNKEGKSLVDLCNRWNNTQCSNYL
                     MKNGVVARIKPSTSSENNGDKKVRTSFSRNYYGKTYSNGKTVEMYANISVSPNEIKIY
                     NEEGSLLHKCTVIKSKYIKENGIDGIEYYTKHQSGSNLYIYFYEMPNFTYYSNGVQAS
                     ITGWNRCVLKNPGESSLILDAQW"
     gene            complement(3402..>3691)
                     /locus_tag="PLU36_09060"
     CDS             complement(3402..>3691)
                     /locus_tag="PLU36_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HMZ46938.1"
                     /translation="IINKARSAELRPEQKDSDSLPDYTILDNILYQYIEMRLGPSEIK
                     ALGFEAATVDRILKMVNNNEYKRNQFCPIIRISPKAFGMGRRMPIVGKYLS"
BASE COUNT         1273 a          531 c          536 g         1351 t
ORIGIN      
        1 ttcgtgattg gttaagacga gaaaaaagtg ggaaagaaac agaagaatca acaatcgact
       61 gggatgatgt agtttttgaa gttgacttac tcaaatcaca agagataaac cttgactata
      121 ttctagaatt gatatttgag cataataaga aaactaaaga caaagacact ttgattaccg
      181 aaatacgcag agttattcgt gcaagtgtag gaaatagagc caaagaaagt ttggttgttg
      241 atttcatcaa tgagactgac cttgacacaa ttcaagacaa agccaatgtg atagattcat
      301 tttttgtatt tgctcaaaac aagcagaaaa cggaagcatt ggaattaatc acagaagaaa
      361 atctgaatgt tgaagaagca aaacgataca taacaacttc attaaagcga gaatttgcaa
      421 gtgaaaacgg aacagaactc aatgcacttt taccgaaaat gagtccatta aatcctcaat
      481 acttgacaaa aaagcaaagc gtttttcaaa agttagtttc gtttgtagag aaatttaaag
      541 gagttggagg acaactgtaa aaaactaaaa gtaaaatttg agcatttaaa tgttttatga
      601 cattacttcc aatggcaact gtatttgaaa tttaaaagac atattgatta aagcagaaat
      661 tacattttat atataagaac attacttttt taaaaacttt cctaagcttc tatattaaat
      721 agctaaaatt agagtaaggt taacccacca ttttttataa aataggcttg ttctttttca
      781 ttttttgttt tattttatag aattgagcaa gttccttttt atttccattc tacaaaaacc
      841 tatttttata actttattaa tcttgttttt tattaatgaa gtatttatcc atctgcttat
      901 ttattttatc taatattttt tttacgcatg tttttgccca aaatagtaca tctaacttta
      961 agaaacttaa tataataaat accacaaacg attctggatt tttaactatt ggctcaatta
     1021 atattaatgg caataaaaga acaaaagatt atataatatt aagagaagtg ccttttaaat
     1081 tagggaataa atacaaacca catgaactta cagggttgtt agaattggca agaaatcaaa
     1141 taatgaacac tgcactattt gtagatgcac aagtttatgt aacaaattta ttaaaagata
     1201 ctgcttttat aaatattgat gtgaaagaaa ggtggtacat ttttcctatt ccttatttta
     1261 atttggttga tagaaatttt aatcagtgga ttaatgagca taaagcaagc cttgataggg
     1321 tgaattatgg aataaaattt tttcatttca atgttagtgg tagaaatgat aaactttcgt
     1381 tagatgtgat tggaggctat agccaacagc tacaatttaa ttataccaac ccttttgcag
     1441 ataaaacttt aaagcatggt ttctctgttg gttttggtta tgtaaggcaa aaagagttaa
     1501 actatgcctc tttaaataat aagcaggtat ttgtacgtac caatagtttt gttagagatg
     1561 gatttagaag cttactcact tattcttatc gcccagattc taaatatcgt ttttatgtta
     1621 gcttaggctt aaatatggat aaaatttctg acacagtttt aaaattaaac cctacttatt
     1681 ttccctacta taccaataaa attacatatc ctaccttatt ggtaatgcta caatattttg
     1741 gggtagatta tattcctttt ccaaaaaaag gttttttgta tcaggtaagc atgtttaata
     1801 gaagttttaa caaaaaggca aacctttttc agttgagtgt aaaaacaacc aatgtattac
     1861 cattagctac aaatagtttt ttgaacatac atactaatag cattttaaaa attggagata
     1921 atagaagtta ttttaaccaa tcgttaatgg ggtataacga ggttttttta agaggcttag
     1981 agtcttatgt tattgatgga atggctggcg ttgtagccaa caattccttg tatcaaaaac
     2041 tatttaagtt tgtttttaaa aatcctatta aaataaaagg gcatgaaaat attccatttc
     2101 atttttatgc aaaactgttt agcgattttg ggtatgctta taataaatat gcagcagtta
     2161 ataacccttt agcaaataag tttttatata ctgctggtgc aggaattgat attactacta
     2221 tatatgattt tgtttttaga attgaatatg ggctaaatca attaggtaat acagcttttg
     2281 gacttagagt taaaggagag ttttaactac cattgtgcat ctaatattaa agaactctcc
     2341 cctgggttct ttaatacaca acggttccag cctgtaatac ttgcttgtac accattgcta
     2401 taatatgtaa aattgggcat ttcataaaag tatatgtata aattacttcc agattgatgt
     2461 tttgtgtaat attcaatacc atcaatgccg ttttctttta tgtatttgct ttttattaca
     2521 gtacatttat gaagtaaaga accctcttca ttatatattt ttatttcatt aggactaaca
     2581 gaaatgttag catacatttc tactgtttta ccattactat aggttttgcc ataataattt
     2641 ctactaaaag aagttcttac ttttttgtct ccattatttt cactcgaagt ggaaggtttt
     2701 atacgagcca ctactccatt tttcattaaa taatttgaac attgtgtatt gttccatctg
     2761 ttacacaaat caactaaact ttttccttct ttgtttaata tgaaaatgtc tgcacctttt
     2821 ttatgtaaat actctagaat ttttattgtt ttattatagg gatcgttgtt agtaagcaat
     2881 gcaattaaag gtgtttcatt taagtcatct actgcattta catctaagcc attttttacc
     2941 aatatttcta cacaatcata agatgtttta aaacacgcta ggtgtaataa acgaccaact
     3001 tttacattag caccggcttt tattaaaggc tctatgtatt tacaggcatt tgcacgaatt
     3061 gcagatacca aaggagtatt atggtattta ctattaattt ctgcatttac atcagctcct
     3121 aattgtataa gtgtttcaaa catttcattt tctgcacttc ctaacccata attattatat
     3181 tcaaaaatgg catgtaaggg tgtaaaattt tgactgtttc ttgcattaat attggctccc
     3241 tttttaatag ctttatgaaa cttttttaca tctttttctt ttatggcagc aaacaaatta
     3301 tcatcttgtt tattgggctg tgtaaaagca acattgctta aaattaaaat agataaaaaa
     3361 ctaaaaacaa ataaacgcat attaaaataa atttttaaaa atcaacttaa atatttacct
     3421 acaatgggca ttcttctacc catgccaaaa gctttagggc ttattctaat aattgggcaa
     3481 aactgatttc ttttatattc attgttgttt accatcttta aaattctgtc cacagttgca
     3541 gcctcaaagc ccaatgcttt tatttcagat ggtcctaatc tcatttcaat atactgatac
     3601 aaaatattat ctaaaatggt ataatccggt aagctatcac tatctttttg ttcaggtctt
     3661 aactctgcac ttcgtgcttt gtttataata t
//
LOCUS       DAOQPK010000214         3686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_17317_length_3686_cov_3.894519, whole genome shotgun sequence.
ACCESSION   DAOQPK010000214 DAOQPK010000000
VERSION     DAOQPK010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17317_length_3686_cov_3.894519"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..889)
                     /locus_tag="PLU36_09065"
     CDS             complement(95..889)
                     /locus_tag="PLU36_09065"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HMZ46939.1"
                     /translation="MQQYQQLLQHIINTGERKEDRTGTGTISCFGYQMRFDLQKGFPL
                     VTTKKLHLKSIIYELLWFLNGDTNIKYLNNNGVSIWNEWANDNGDLGPVYGKQWRSWQ
                     GANGKTIDQIADVIKQLKTNPDSRRMIVSAWNVAELSEMALMPCHALFQFYVANGKLS
                     CQLYQRSADVFLGVPFNIASYAALTMMIAQVCNLQLGEFIHSFGDVHLYCNHIEQAQL
                     QLTRTPFELPILKINSSVKDIFSFQYEDFILENYQFHPHIKAVVAV"
     gene            1124..1642
                     /locus_tag="PLU36_09070"
     CDS             1124..1642
                     /locus_tag="PLU36_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46940.1"
                     /translation="MKSVKYYIHFVWMLLFSVVAVNAQNSFNPTGYATSLKEALKEPE
                     NISTISVYYTRGMVFDIEQGKAAPDASFEKLMELNNLRTFRLNGCPLDFNQELFFCNL
                     SKVKQLEALELRMNFRQLGLLTEQSIKCLKKLKNLRRLNLPCQYPQEDYIKLQQQLPD
                     CEIIINVYPGGE"
     gene            complement(1656..2741)
                     /gene="recF"
                     /locus_tag="PLU36_09075"
     CDS             complement(1656..2741)
                     /gene="recF"
                     /locus_tag="PLU36_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626633.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HMZ46941.1"
                     /translation="MLQLNNIALLQFKNYTQQQFVFTQKVVGICGRNGSGKTNLLDAI
                     YYLCFTKSYFLASDAQNVQIGKAGMRIEGNMLLNNTTQNLICILRENNKKEFLYNKEQ
                     LKKFSEHIGRFPCVIISPDDTTLVTGSSELRRKYIDTIIAQINSHYLYNLVDYNKILV
                     QRNSLLKQAAEVGTINKDVVEILNQQLATKANYIFEERKLFFKQYIPFVYDYYSKIAS
                     KTDNITLHHQSALYTETIENLLEQNLQKDIALQRTTAGIHRDDIVINMNGALFKTIAS
                     QGQRKSLLFALKLAEWQIIKQKKLYSPILLLDDVFEKLDGERMHNLLQWVCNEESTQV
                     FITDTHKERLEQALQNAAINFQLIELQ"
     gene            complement(2741..>3686)
                     /locus_tag="PLU36_09080"
     CDS             complement(2741..>3686)
                     /locus_tag="PLU36_09080"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HMZ46942.1"
                     /translation="KGDSTKICSCDTSDIAGFNASFSANDRTRTFLKVQDGCDYNCSF
                     CTIPMARGKSRSDSISNVLNSAKELASKGVKEIVLTGVNLGDFGKGSQGAGVSISMQE
                     REEDFFDLIQALDAIDGIERFRISSIEPNLLTNPIISFVAKSKKFMPHFHIPLQSGSN
                     HILKLMRRRYQRELYQDRVAYIKSLMPHCSIGVDVIVGFPGETDTYFEETFNFLHNLD
                     VSYLHVFTYSERDNTVALNLKPVIPVNIRHQRNKTLRNLSFMKLQYFTQQHSGQNRKV
                     LFESADKNGMMEGYTDNYIRISTPFRKEWENQIIDWKI"
BASE COUNT         1192 a          594 c          523 g         1377 t
ORIGIN      
        1 ttttaccaat tacattattc tcactagcgg caacaattaa tgaaataatc atgaaaaaat
       61 ttttatttta tcttaataat aaccaactta attattaaac tgctaccact gctttaatat
      121 gtggatgaaa ctgataattt tctaaaataa aatcttcgta ttgaaaacta aaaatatctt
      181 ttacagaaga gttaattttt aatattggca gctcaaacgg tgttctggtt aattgtagtt
      241 gtgcttgttc aatatgatta cagtataaat gtacatcgcc aaagctatgt ataaattcac
      301 ctaattgtaa attacaaact tgagcaatca tcattgttaa agctgcataa gatgctatat
      361 taaatggtac acctaaaaat acatctgcac tacgttgata taactgacaa cttaatttac
      421 cattagccac ataaaactga aaaagtgcat ggcaaggcat taaagccatt tcagaaagtt
      481 ctgccacatt ccaagcactt acaatcattc tcctgctatc tgggtttgtt tttaattgct
      541 taattacatc ggctatttga tctattgttt taccatttgc accttgccag ctacgccatt
      601 gtttgccata aacaggccca aggtctccat tgtcatttgc ccactcgttc caaatactta
      661 cgccattatt gtttaaatat ttaatgtttg tatcgccatt taaaaaccaa agtaactcgt
      721 aaataatact ttttaagtgc aatttttttg tggttactaa cggaaaacct ttttgtaaat
      781 caaaacgcat ttgatatcca aaacaactaa ttgttccggt acctgtcctg tcttcttttc
      841 tttctccagt attaatgata tgttgtaata gttgttggta ttgttgcata agtgcaaatt
      901 aaacaatgtt ctattgtaaa tattcaatac aaaaataaat ggggaatagt tgtgtaaaga
      961 cttatttaat tataaaaatt tagttatggt tttagaagat ggtaccatgt atattaatgt
     1021 gcataattgc aataaatatt tttctttatg cacaacaagc ccaaaattta agttgaacaa
     1081 aaaatgcttt ttacatttac attgcttact aatcccttta tctatgaagt ccgtaaaata
     1141 ttacatccat ttcgtatgga tgttactttt ctcggttgtg gcagtaaatg cacaaaatag
     1201 ttttaaccca acaggttatg ctacttcttt aaaagaagca ttgaaggagc ctgaaaatat
     1261 ttctaccatt agtgtatatt atacacgagg aatggtgttt gatattgagc aaggcaaagc
     1321 tgcaccagat gcctcttttg agaagttaat ggagttaaac aacctaagaa cttttagatt
     1381 aaatggttgt ccgttagatt ttaatcagga gttatttttt tgtaacctta gtaaagtaaa
     1441 acaattagag gctttagagt taagaatgaa ttttagacaa cttgggttac taacggaaca
     1501 atctattaaa tgtttaaaaa agctgaaaaa cctacgcagg ctgaatttgc cttgtcagta
     1561 tccgcaggaa gactatatta agttgcaaca acaattacca gattgtgaaa ttattattaa
     1621 tgtgtatcca ggaggagagt aaaatctcct cttttttatt gaagttctat taactgaaaa
     1681 ttaattgcag cattttgcaa tgcttgttct aacctttctt tatgagtatc agttataaat
     1741 acttgtgtgc tttcttcgtt acaaacccat tgcaataagt tatgcattct ttcaccatct
     1801 aatttttcaa aaacatcatc taacaataat attggagagt ataatttttt ttgttttata
     1861 atttgccatt ctgccaactt taatgcaaac aacaaacttt ttctttggcc ttgagatgca
     1921 atagttttaa ataatgcacc attcatattt attactatat catctctatg aataccagcg
     1981 gttgtacgtt gtaaagcaat atctttttgt aggttttgtt ctaataaatt ctctatagtt
     2041 tcggtgtata atgcactttg atgatggagg gtaatattat cggttttact agcaattttg
     2101 ctataataat cgtacacaaa tggtatgtat tgcttgaaaa atagtttacg ttcttcaaaa
     2161 atataatttg ctttagtagc aagttgttga tttaatattt ctactacatc cttattaatt
     2221 gttcctactt cggcagcttg ttttagtaaa ctatttcttt gaactaatat tttattgtaa
     2281 tctactaaat tgtataaata atgagagttt atttgagcaa taattgtatc tatatatttc
     2341 cgcctaagtt cgctactacc tgttactaaa gttgtatcat ctggtgaaat aattacacaa
     2401 ggaaatctac caatatgttc tgaaaatttt tttagttgct ctttattata caaaaattct
     2461 tttttattat tttctcttaa aatgcaaatt aaattttgag tagtattgtt caatagcatg
     2521 tttccttcaa ttctcattcc tgcttttcct atttgtacat tttgagcatc agaagctaag
     2581 aagtagcttt tagtaaagca caggtaataa attgcatcta ataaattagt tttacccgag
     2641 ccgttccttc cacaaatacc tactaccttt tgagtaaata caaattgctg ttgagtataa
     2701 tttttaaatt gtaataaagc tatattattt aattgtagca ttatattttc caatcaataa
     2761 tttggttttc ccattctttt ctgaatggtg tagaaattct aatgtagtta tctgtatagc
     2821 cttccatcat tccattttta tcggcagatt caaataacac ttttctgttt tgcccactat
     2881 gttgttgtgt aaaatattgc agtttcataa aacttaaatt acgcaaagtt ttatttcgtt
     2941 gatggcgaat atttacagga attacaggct ttaaatttaa agctacagta ttatctcttt
     3001 cgctataggt aaaaacatgt aaataactaa catctaagtt gtgaagaaag ttaaatgttt
     3061 cttcaaaata tgtatccgtt tctccaggaa agccaacaat aacatcaaca cctatggagc
     3121 aatgtggcat aagtgattta atgtaagcaa ctctatcttg atacaactct ctttgatacc
     3181 ttcttctcat taattttaaa atatggttgc taccactttg taaaggaata tgaaaatgtg
     3241 gcatgaattt tttacttttt gccacaaaag aaattattgg attggttaat aagtttggct
     3301 ctatagagga tattctgaaa cgttcaatac catctatagc atctaatgct tgtattaaat
     3361 caaaaaaatc ttcttctctt tcttgcatgc ttatagatac tcctgcacct tgtgaacctt
     3421 tgccaaaatc tccaaggttt acacctgtta aaacaatttc ttttactcct ttactagcaa
     3481 gttctttagc actatttagt acattgctaa tgctatcact tctgctttta cctcttgcca
     3541 ttggtatggt gcaaaagcta caattataat cgcaaccatc ttgtactttt aaaaaagttc
     3601 ttgtacgatc gttggcagaa aaagaagcat taaaacctgc aatatcgcta gtatcgcaac
     3661 tgcaaatttt ggtgctgtct cctttg
//
LOCUS       DAOQPK010000215         3678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_17389_length_3678_cov_4.731714, whole genome shotgun sequence.
ACCESSION   DAOQPK010000215 DAOQPK010000000
VERSION     DAOQPK010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17389_length_3678_cov_4.731714"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PLU36_09085"
     CDS             complement(<1..233)
                     /locus_tag="PLU36_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HMZ46943.1"
                     /translation="MTHPLTYIDPNAKIAANVKIDPFTVIHDNVKIGEGTWIGSNVTI
                     MEGTRIGKNCRIFPGAVIGAIPQDLKFNGEKTNV"
     gene            complement(296..1699)
                     /locus_tag="PLU36_09090"
     CDS             complement(296..1699)
                     /locus_tag="PLU36_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HMZ46944.1"
                     /translation="MNTTFNPDKQHSIATSVSISGTGLHTGILADMTLLPANPGFGIQ
                     FQRTDLPNKPIIKADCDYVTDTSRGTTLQSGDAKVNTVEHILAALVGMGVDNCLIEIN
                     GPEIPIMDGSSKDFVDIISTVGVVEQDAAKAWYSIDENIIYYDESKRVEMVAMPAMDY
                     QITTLIDFNSNVLGTQHAGLKTIKDFSQEIAPCRTFCFLHELEMLLDNDLIKGGDINN
                     AIVVVDKPISKEEMERLAVAFKRDKIEVKSEGYLNNLELRFPNEPARHKLLDIIGDLA
                     LIGYPIKARIIANRPGHSTNVEFAKRIKQYIKRNKHVKDVPVYDPLIPSVFTLEQIEK
                     TLPHRHPFLLVDKIVELTDTQIVGVKNVTFNEWFFPGHFPGNPVMPGVLQIEALAQTG
                     GILCINAMPKGEYNTYFLKIDNCKFKQMVKPGDTMLLKMELVSPIRRGICEMKGTVYV
                     AGKVATEAILVAQVIKK"
     gene            1924..2802
                     /locus_tag="PLU36_09095"
     CDS             1924..2802
                     /locus_tag="PLU36_09095"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HMZ46945.1"
                     /translation="MKIAIYSRGLDASLENPLVYLLNELVKYNIEIVVYRQLPQLYHL
                     PNELKSIISTFQFSEELMPDIDCLISLGGDGTMLDAVALVKDKNIPILGVNIGRLGFL
                     ANINKDELSLAVDAIINRTYTIDKRSLIHVDANVGLFNDAPFALNEFAILKRDTSPMI
                     KIHTYLNGEFLNTFWADGLLVSTPTGSTGYNMSCNGPIVFPDASNFIITPIAPHNLNV
                     RSIVVSDSNIISFEIEGRAEQFICALDARREIVNKEVQLAVKKEDFYINIIRLNEGSF
                     LSTLRNKLTWGMDKRN"
     gene            2906..>3678
                     /locus_tag="PLU36_09100"
     CDS             2906..>3678
                     /locus_tag="PLU36_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HMZ46946.1"
                     /translation="MFKKVITFCVLSIFSFQVKAQVMDAFVHEGEFGIGVGAGHYFGD
                     LTAGMKLIDIKPKLSATIFFRKQFNNYIGIKIAGTFAFLGYADKYTSYKDNLVKKNRN
                     LSFNTNIWELSINGEFNFFRFQPGFSEYRFTPYVSLGIGAFAYDPYAYLYGAKHYLRP
                     LGTEGQQDKVNYPNLKPYSNMAMCFPIGFGLKYSLSEKINIYGEMVYRLTNTDYIDDV
                     SGNYAPDAFPPNPNGTPSVAYSLQDRSYEYGLPIGIKGR"
BASE COUNT         1225 a          559 c          551 g         1343 t
ORIGIN      
        1 acattcgttt tttcgccatt aaattttaaa tcttgtggta ttgcaccaat tactgcaccc
       61 ggaaaaattc tgcagttttt acctattctt gttccttcca ttattgtaac attgctacca
      121 atccaggttc cttctccaat ttttacatta tcatgtataa ccgtaaaagg gtctattttt
      181 acgttggctg caattttagc atttgggtca atatatgtta atggatgggt catacaaatt
      241 tatttatttg aaagagaata gggtttatga aaaatattta tcaaaattag tttccttatt
      301 ttttaattac ttgggctact aaaattgctt ctgttgcaac ttttcctgct acataaacag
      361 tacctttcat ttcgcaaatg cctcttctta taggcgatac taattccatt tttaaaagca
      421 tagtatcccc tggttttacc atttgtttaa atttacaatt atctattttt aagaaataag
      481 tattgtattc acctttaggc attgcattaa tacacaaaat gccaccagtt tgtgctaaag
      541 cttcaatttg taaaactcct ggcattacag gatttcctgg aaaatgacct gggaaaaacc
      601 attcgttaaa agttacattt ttaactccta caatttgggt atctgttagc tccacaattt
      661 tatcaaccaa taagaaagga tgacgatgtg gtaatgtttt ttcaatttgt tctaaagtga
      721 aaacagaagg aattaaagga tcataaacag gtacatcttt aacgtgttta tttcttttta
      781 tatattgctt tattcttttg gcaaactcca cattagtact atgtcctggc ctattggcta
      841 taattctagc tttaatagga tagccaatta atgcaagatc tccaataata tctaaaagct
      901 tatgccttgc aggctcatta ggaaaacgaa gctctaaatt gtttaagtat ccttctgatt
      961 ttacttctat tttatctctt ttaaatgcta ctgcaagcct ttccatctct tcttttgaaa
     1021 ttggcttatc tacaaccaca atggcattat taatatcgcc accttttatt aaatcattat
     1081 ctaatagcat ttctaattca tgcaaaaaac aaaatgttct acaaggtgca atttcctggc
     1141 taaaatcttt aattgttttt aatccggcat gctgtgtacc taaaacatta ctattaaaat
     1201 ctattaaggt agttatctga tagtccattg caggcatagc taccatttct actcttttag
     1261 actcatcgta gtaaataata ttttcatcaa tactatacca tgcttttgca gcatcttgtt
     1321 caaccacacc aactgtagaa ataatatcta caaagtcttt gctgctcccg tccattattg
     1381 gtatttctgg tccgtttatt tctattaagc aattatccac acccatacct accaatgcag
     1441 ctaaaatatg ctctacagta ttaacttttg catcgccact ttgtaaagta gtacctctag
     1501 atgtatccgt tacataatca caatctgcct taattatagg cttattaggc aaatctgttc
     1561 tttgaaattg aataccaaaa cctgggttag ctggcaataa agtcatatct gctaaaattc
     1621 cagtatgtaa acctgttcca ctaatactta cactggttgc aatactatgt tgcttatcag
     1681 gattaaaagt agtattcata aatagtttat taattgcagg aaataaaagt aatatatatc
     1741 aaaatataat tttttaaccc tacattaaat aaagggcaaa aataagtgtt agtgaacaaa
     1801 ttaaagttca atattttaca ttgattttat aaattttatc caaaataata tatttattgc
     1861 attaaggtta ttaagtatct ttaccgcttc aataaaaaat gctacaccat ttaaaaaact
     1921 tgaatgaaaa tagctattta tagtagaggg ttagatgcaa gtttagaaaa tccactagtt
     1981 tatttattaa atgaattggt aaaatataat attgaaattg tagtttatag gcaattgcca
     2041 caattatatc atttacctaa cgaattaaaa agtattattt ctacttttca attttcagaa
     2101 gaattaatgc cagatataga ttgtttaata agccttggtg gagatggaac tatgctggat
     2161 gcagttgctt tagtaaaaga taaaaatatt ccaattttag gtgtaaatat tggcaggcta
     2221 ggctttttag caaatattaa taaagatgaa ctttctttgg ctgtagatgc aattataaat
     2281 agaacttata ctattgataa acgatctctt atacatgtag atgctaatgt tggtttgttt
     2341 aacgatgccc catttgcatt aaatgaattt gctatactaa aaagagatac ttcaccaatg
     2401 attaaaattc atacttactt gaatggagaa tttttaaata ctttttgggc agatggtttg
     2461 ttagtttcta ccccaacagg tagtacaggg tataatatga gttgtaatgg gccaattgta
     2521 tttcccgatg catcaaattt tattataaca cctattgcac cccataactt aaatgttaga
     2581 agtattgttg tatctgatag taatattatt tcttttgaaa tagaaggtag ggcagaacaa
     2641 tttatttgtg cattagatgc cagaagagaa attgtaaata aagaggtaca attagcagta
     2701 aaaaaagaag atttttatat aaatattatt cgtttaaatg aaggtagttt tttatctact
     2761 ttaagaaata agctaacctg gggaatggat aaaagaaact aaacccaata ttttttatgt
     2821 tttgtattaa aattttaact caataattat aagaaaatat atctaatttt cgttttaacc
     2881 cttattagta cgttaatttg cccctatgtt taaaaaagtt attacttttt gtgttttaag
     2941 tattttttca tttcaggtaa aggcacaagt tatggatgcc tttgtacatg aaggtgaatt
     3001 tggaattggt gttggagcag gtcattattt tggcgactta actgctggta tgaaattaat
     3061 tgatattaaa cctaaactat cggcaactat tttttttcgt aaacaattta ataattatat
     3121 aggaataaaa atagctggaa cttttgcttt tttaggctat gcagataaat acactagcta
     3181 taaagataat ttagtaaaaa aaaataggaa cttaagcttt aacactaata tttgggagct
     3241 tagtattaat ggcgaattta atttctttcg ttttcagcct ggtttttcag aatatagatt
     3301 taccccttat gtatcattag gcattggtgc ttttgcttat gacccttatg cctatttgta
     3361 tggtgctaaa cattatttaa gaccacttgg tacagaaggg cagcaagaca aggttaacta
     3421 tcctaattta aaaccatata gcaatatggc aatgtgtttt ccaattgggt ttggtttaaa
     3481 atatagcctt agcgaaaaaa ttaatattta tggagaaatg gtgtataggc ttactaatac
     3541 agattatata gacgatgtaa gtgggaatta tgctcctgat gcttttccac caaatccaaa
     3601 tggtacacca tcggtagcat attcattgca agatagaagt tacgaatatg gcttaccaat
     3661 tggaattaaa ggcagaca
//
LOCUS       DAOQPK010000216         3654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_17598_length_3654_cov_5.177827, whole genome shotgun sequence.
ACCESSION   DAOQPK010000216 DAOQPK010000000
VERSION     DAOQPK010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17598_length_3654_cov_5.177827"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PLU36_09105"
     CDS             complement(<1..484)
                     /locus_tag="PLU36_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HMZ46947.1"
                     /translation="MALIKSISGIRGTIGGKPNETLSPLDIVRFSASYGTWLLNNNTE
                     KIKVVIGRDGRISGPMVQSIVTNTLIALGIDVVDVGISTTPTVEMAVVFHQANGGIII
                     TASHNPKEWNALKLLNNKGEFISAKEGETILDIASKDLFAFASVDKLGTVINDETALD
                     K"
     gene            724..1404
                     /locus_tag="PLU36_09110"
     CDS             724..1404
                     /locus_tag="PLU36_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46948.1"
                     /translation="MKTIFFLGFFIISTVAIAQNKIVGTWKNDQAGTLILNANGTGSF
                     EGDAFTYTNNAKKIFVNFDGEVTTYSYYFNNGQLVVSGGDFVQPIAFTNTKSSGNPTG
                     KSKNSGGGGIDKSIVGTWCWTNASNNSYTSSSNTRCIVINADGTYTYTYEGSISGYGG
                     GGYGGSNSQNSDRGTWRVNGNTISVVSESSGAKTYSFQKRNHPKTGDPMIIIDGDAYV
                     TYYKKSPW"
     gene            1478..2227
                     /locus_tag="PLU36_09115"
     CDS             1478..2227
                     /locus_tag="PLU36_09115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ46949.1"
                     /translation="MRKEAWYKDWFSSPYYHQLYQYRDETEAFAFIGTLIQHLKPAKN
                     SIMLDVACGKGRHSIALAEMGFDVTGIDLSLSSIEEASLSEANNLHFFQHDMRLPFWI
                     NYFDYAFNFFTSFGYFKTEREHNNAIRTIATSLKKNGFFVIDYLNTHYAEDTLQPSAI
                     TKVDGTEFHITKWHDEEHFYKQIQITDNTNFTPKHLYTEKIAKFSLGDFNEMLSYQNM
                     QIQEVFGDYKLGSFDIKKSPRMIIIAKKISK"
     gene            2259..3011
                     /locus_tag="PLU36_09120"
     CDS             2259..3011
                     /locus_tag="PLU36_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HMZ46950.1"
                     /translation="MELKKFLDKKANQYNQPSFISTDPICIPHLFKQQQDIEIAGFFA
                     AIFAWGNRKIIINKSKELMQLMQMKPYHFILQHNNNDLKKLLHFKHRTFNATDLLYCI
                     AFLKHHYTYHKSLETAFTQWGNTVEEMLIGFHQYFFSLPDYPERTKKHIATPAKNATC
                     KRLNMFLRWMVRRDNNGVDFGLWKNISMAQLICPVDVHVARVAIKLNLIKNKPANWKT
                     AKELTLYLKTLNSKDPVKYDFALFGLGIMEKY"
     gene            complement(3035..>3654)
                     /locus_tag="PLU36_09125"
     CDS             complement(3035..>3654)
                     /locus_tag="PLU36_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HMZ46951.1"
                     /translation="FDEPMGGLTKEQIKKAKSQPNISLADFINPANSNKEVKDYIGAF
                     AVTIHGIEPHIKAFIDNYDDYNKISLQALADRLAESLAEYLHEKVRKEFWGYVKEESL
                     TNEDLIKEKYQGIRPAPGYPACPDHTEKNKLFSLLNATENTGIVLTEHFAMYPASSVC
                     GWYFSNPQSQYYGVGKIQQDQFEDYVQRKKMPKEELERWLSSNLE"
BASE COUNT         1245 a          566 c          557 g         1286 t
ORIGIN      
        1 gtttgtctaa agcagtttcg tcatttatta ctgtgcctag tttatctaca cttgcaaaag
       61 caaataaatc tttacttgca atgtctaaaa tagtttcgcc ttcttttgca ctaataaatt
      121 cgcctttatt atttagtagt tttaatgcat tccattcttt agggttatgg cttgcagtaa
      181 taattatacc tccattagct tggtgaaaaa caactgccat ttccacagtt ggcgtagtgc
      241 ttataccaac atctaccaca tctataccta atgcaatcaa tgtattggtt acaatgcttt
      301 gaaccattgg gccactaatt ctaccatctc taccaataac tacttttatt ttttctgtat
      361 tgttattcaa taaccaggtg ccataggatg cagaaaatct aacaatatct aaaggactta
      421 gtgtttcatt tggtttaccc cctatcgttc ctctaattcc agaaatactc tttattaacg
      481 ccattaaacc ttttttaagg tggcaaacat accaaaaact agcttacttt tttttattta
      541 tataattaaa gagcattagt aaagtgtttt ttaaagaagt tttatagtta cagaaaacta
      601 ctaaaaataa ttttttgttg aaaatcagta agtttagcaa ccattaaaca aaaaatggag
      661 gagataaaat aaattacctt tttgtattta atttttataa ttttaggtat aaaataaact
      721 gatatgaaaa ctatattttt tttaggtttt tttataattt ctacggtagc aatagctcaa
      781 aacaaaattg ttggcacttg gaaaaatgac caagcaggta cattaatttt aaatgcaaat
      841 ggtacaggaa gttttgaagg tgatgctttt acatacacca ataatgcaaa aaaaatattt
      901 gtaaattttg atggagaagt aactacttac tcttattatt ttaataatgg gcagttagtc
      961 gtttctggtg gcgattttgt acaacctatt gcttttacaa acacaaaaag ttctggaaac
     1021 ccaacaggaa aatctaaaaa cagtggtggt ggtggaattg ataaatctat tgtgggtact
     1081 tggtgttgga ccaatgcatc caacaatagt tatactagtt ctagcaatac cagatgcatt
     1141 gttattaatg ccgatggaac atatacttac acttacgaag gttctataag tgggtatggt
     1201 ggtggtggtt atggtggttc taacagccaa aattctgaca gaggaacatg gagggtaaat
     1261 ggcaatacaa ttagtgttgt ttctgaaagc tcgggagcta aaacctattc ttttcaaaag
     1321 agaaatcacc ctaaaaccgg cgaccccatg attattattg atggagatgc ttatgtaact
     1381 tattataaaa aatccccttg gtaattttct tacacttgca ttttttaaaa atacttttta
     1441 gcttacatca ttacctttgc tttagtaaaa aggttttatg aggaaagaag cttggtataa
     1501 agattggttt tcatcgccat actatcatca actatatcaa tacagagacg aaacggaagc
     1561 atttgctttt attggcactt taatacaaca tttaaagcca gcaaaaaaca gtataatgtt
     1621 agatgttgct tgtggcaaag gcaggcacag cattgctttg gcagaaatgg gctttgatgt
     1681 tacaggtatt gacctttctc tttcctctat tgaagaagca agccttagcg aagcaaataa
     1741 tctacacttt tttcagcatg atatgagatt gcctttttgg attaattatt ttgattatgc
     1801 ctttaatttt tttaccagtt ttggatattt taaaactgaa cgtgaacata ataatgctat
     1861 tagaacaatt gctaccagct taaaaaagaa tggctttttt gtgatagact atttgaatac
     1921 gcattatgcc gaagacactt tacaacctag tgctattact aaagttgatg gaaccgaatt
     1981 tcatataaca aaatggcacg atgaagaaca tttttacaaa caaattcaaa taaccgataa
     2041 tactaacttt actcctaaac atttatatac cgaaaaaatt gccaaatttt ctttaggcga
     2101 ttttaatgaa atgctttctt atcaaaacat gcagatacaa gaggtttttg gagattataa
     2161 acttggtagt tttgatataa aaaaatctcc aagaatgatt attatagcta aaaaaataag
     2221 taaataatag cttacttttt tgtagttaaa agtaatgtat ggagttaaaa aaatttctag
     2281 ataaaaaagc taatcaatac aatcaacctt catttatcag tacagatcct atttgtattc
     2341 ctcatttatt taagcagcaa caagatattg aaattgcagg tttttttgca gccatttttg
     2401 catggggtaa tagaaaaatt attattaata aaagtaaaga gttaatgcag ttaatgcaaa
     2461 tgaagccata tcattttata ctgcaacata acaataacga tttaaaaaaa cttctacatt
     2521 ttaaacacag aacttttaat gcaacagatt tgctatactg tatagctttc ttaaaacacc
     2581 attatacata tcataaaagt ttagaaactg cttttacaca atggggcaat acagtagagg
     2641 aaatgcttat tggttttcat caatattttt tttctttacc cgattatcct gaaagaacta
     2701 aaaaacatat tgcaacacca gcaaaaaatg caacctgcaa aagattaaat atgtttttaa
     2761 gatggatggt tagaagggat aataatggtg ttgattttgg tttatggaaa aatatttcta
     2821 tggcacagct tatttgccca gtggatgtac atgttgcaag agttgccata aagctaaact
     2881 taattaaaaa caaacctgcc aattggaaaa ctgctaaaga acttaccctt tatttaaaaa
     2941 ccttaaacag caaagaccct gttaaatacg attttgcatt atttggttta ggtataatgg
     3001 aaaaatatta aaatattttt aagttaaaaa tggtttattc taaattagag cttaaccatc
     3061 tttctaattc ttctttaggc attttttttc tctgtacata atcttcaaac tgatcttgtt
     3121 gaattttgcc tacaccataa tattggcttt gtgggttact aaagtaccaa ccacatacac
     3181 tgcttgcagg atacatggca aaatgctcag taagtacaat acctgtattt tctgtagcat
     3241 ttaataagct aaataattta tttttttctg tatgatctgg gcatgcaggg taaccaggtg
     3301 ctggacgaat accttggtat ttttctttaa ttaaatcttc gttagttaaa ctttcttctt
     3361 taacataacc ccaaaattct tttcttactt tttcatgtaa atattctgct aaagattctg
     3421 caagcctatc tgctaatgcc tgtaagctaa ttttattata atcgtcgtaa ttatctataa
     3481 atgcttttat atgaggttca ataccgtgta tagttacagc aaaagcacca atataatctt
     3541 ttacttcttt attactattg gcagggttaa taaaatctgc taatgaaata ttgggttggc
     3601 tttttgcttt tttaatttgt tccttagtta aaccccccat gggctcatca aaat
//
LOCUS       DAOQPK010000217         3653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_17611_length_3653_cov_3.693997, whole genome shotgun sequence.
ACCESSION   DAOQPK010000217 DAOQPK010000000
VERSION     DAOQPK010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17611_length_3653_cov_3.693997"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            352..708
                     /locus_tag="PLU36_09130"
     CDS             352..708
                     /locus_tag="PLU36_09130"
                     /inference="COORDINATES: protein motif:HMM:NF014015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46952.1"
                     /translation="MFNPLKIEVVEPLYNKTSEEVMEDFLSSGIKAKIIVANYDIFSK
                     DIIGADLNSACVNTMPKGIDICGEQGEYHSFVYEGSIFKTAIKYKIKATKKVAYNIKL
                     DNNKIKTFTYWQAIME"
     gene            complement(710..1063)
                     /locus_tag="PLU36_09135"
     CDS             complement(710..1063)
                     /locus_tag="PLU36_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HMZ46953.1"
                     /translation="MISTNENSIYISDKARAKVAKLMEEAGVANDSSYFLRVGVVGGG
                     CSGLSYKLDFDNEVKPMDQVFENDGIKIVTDLKSFLYLVNTELDFSDGLNGKGFYFSN
                     PNASRSCGCGESFAV"
     gene            complement(1068..1487)
                     /gene="iscU"
                     /locus_tag="PLU36_09140"
     CDS             complement(1068..1487)
                     /gene="iscU"
                     /locus_tag="PLU36_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HMZ46954.1"
                     /translation="MAYSDKVIDHYSNPKNVGTLDKSSKSVGTGLVGAPECGDVMRLQ
                     IEVDDATGLIKDAKFKTFGCGSAIASSSLATEWLKGKSVDEAVKIDNMDIVEELNLPP
                     VKIHCSVLAEDAIKAAINDYRQKNGLEELTFEQKGVH"
     gene            complement(1576..2793)
                     /locus_tag="PLU36_09145"
     CDS             complement(1576..2793)
                     /locus_tag="PLU36_09145"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HMZ46955.1"
                     /translation="MLKLPIYLDNNATTPMDPRVLEAMLPFFTQHFGNAASRNHQFGW
                     EAEEAVDYAREQVAKLVGADAKEIIFTSGATEADNLAIKGVFEMYASKGNHIITVTTE
                     HKAVLDTCKHIEKVGGEVTYLEVNGDGLIDLTLLEAAIKPTTVLIAIMYANNEIGVVQ
                     PIKEIAAIAKKHGILFFTDATQAVGKIPVDVIKDGIDLMAFSSHKMYGPKGIGALYVR
                     RKNPRVKVTAQMDGGGHERGMRSGTLNVPGIVGFGKACEIARLDMEKDTKHIVVLRDK
                     LENALLELEESYLNGNKEHRLPHVSNISFKYVEGEGLMMGFNKNIAVSSGSACTSASL
                     EPSYVLKALGLGDDLAHSSLRFGLSRFTTEEEIDYTIEAVRNTVLKLREMSPLWEMFK
                     EGIDLDTVEWAHH"
     gene            2928..3329
                     /gene="mce"
                     /locus_tag="PLU36_09150"
     CDS             2928..3329
                     /gene="mce"
                     /locus_tag="PLU36_09150"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HMZ46956.1"
                     /translation="MKKVEHIGIAVKDFAISIPLFENLLNVPCYKQETVESENVNTAF
                     FKLGETKIELLQSTNSDGVIAKFIEKKGEGIHHLAFDTDNILAEVERLKNAGFTLLNE
                     TPKEGADNKLVCFLHPKSTNGVLIELCQEKK"
     gene            3396..3641
                     /locus_tag="PLU36_09155"
     CDS             3396..3641
                     /locus_tag="PLU36_09155"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ46957.1"
                     /translation="MFAVTILGNNSAIPAYGRMPTAQVVTLNDQQFLIDCGEGTQMQL
                     AKYKIRKSKINHIFISHLHGDHYFGLIGLITQKLLNH"
BASE COUNT         1214 a          659 c          487 g         1293 t
ORIGIN      
        1 acaccaagca gattcaactc atcttcagct tcttttattt tcattttcat ctgcatcaat
       61 aacggcaaga aaagtttgat tttgatattt gattaaaaca aacctatctc cagaaagtat
      121 caaaacttct aaaaagtttg gatgcaggac tctaaaaatt tgggactctt tgataaattt
      181 tagcaaaacg ccagattcgt taaaaatgat ttagtcggtt ccttgagcga attgatgagt
      241 tgtttgaata agtgtttttg actcttttca aaactttagt ttatagtctc attgtgagtc
      301 tgatcatatt ggtgtgaatc cagttcaaac atattcaact acacgaagaa aatttttaac
      361 ccacttaaga ttgaagtggt agaaccactt tataacaaaa cttctgaaga agttatggaa
      421 gattttttat cctccggtat taaagcaaaa attattgtgg ccaactacga tatttttagt
      481 aaagatatta taggtgcaga tttaaactca gcttgtgtaa acaccatgcc taaaggtatt
      541 gatatttgtg gagagcaagg tgaataccac agttttgttt acgaaggaag tatttttaaa
      601 acagcaatta agtataaaat taaagccact aaaaaagtgg cttacaatat taaattagat
      661 aacaataaaa taaaaacttt tacttattgg caagcaataa tggaataaat taaacagcaa
      721 aactttctcc gcaaccacag ctcctgcttg cattaggatt actaaaataa aacccttttc
      781 catttaaccc atcactaaag tctaattctg tatttactaa gtataaaaaa cttttaaggt
      841 cggtaacaat tttaattcca tcattttcaa aaacctgatc cataggtttt acctcattat
      901 caaaatctaa cttatagctt aagccactac aaccaccacc aactacacct actcgtaaga
      961 aataagatga atcattagca acaccagcct cttccattag ttttgcaact ttagctctag
     1021 ctttatcact tatgtaaata ctattttcat tagtagatat cataaaatta gtgtactcct
     1081 ttttgctcaa aagttaattc ttctaagcca tttttttgac gataatcatt aattgcagct
     1141 ttaattgcat cttctgctaa tacagaacaa tgaattttca caggtggtaa attcaattct
     1201 tccacaatat ccatattatc tatttttact gcttcatcta ccgattttcc ttttaaccat
     1261 tctgttgcta atgaggagga tgcaatagct gagccgcaac caaatgtttt aaattttgca
     1321 tctttaatta atcctgttgc atcatccact tcaatttgta aacgcattac atctccacat
     1381 tctggtgcac ctactaaacc tgttcctaca cttttactac ttttatctaa agtgccaaca
     1441 tttttagggt tactatagtg gtctattact ttatctgaat atgccatata attgatattt
     1501 ttaaattacg attataaaaa atttttagtt tctctgctct aatttatttt gttaaaaaca
     1561 caatactatt ttgttttagt ggtgtgccca ttctacagta tccaaatcta taccttcttt
     1621 aaacatttcc cataacggac tcatttctct cagcttcaac acagtatttc tcactgcttc
     1681 aatggtataa tctatttctt cttctgttgt aaatctgctt aatccaaaac gtagagagct
     1741 gtgggctaaa tcatcgccta agcctaaagc ttttaacaca taacttggtt caagagatgc
     1801 agaagtgcag gcagaacctg aacttactgc aatattttta ttaaagccca tcattaatcc
     1861 ttctccttct acatacttaa aagaaatatt actaacatgt ggtaatcgat gttctttatt
     1921 tccgtttaaa taagattctt ctaattccaa taaggcattt tctaatttat ctcttaatac
     1981 tactatatgt ttggtatctt tctccatatc taatcttgca atttcgcagg ctttaccaaa
     2041 acctacaatt cctggtacat ttaacgtgcc acttctcata cctctttcat ggcctccacc
     2101 atccatttgt gcagttactt ttactctagg attttttctt ctaacatata aagcaccaat
     2161 accttttggg ccatacattt tatggctgct gaatgccatt aaatctatac catcttttat
     2221 tacatcaaca ggtattttac caactgcttg tgtagcatct gtaaaaaata aaatgccatg
     2281 ttttttggct attgcagcaa tttcttttat aggttgtaca acaccaattt cattgttagc
     2341 atacataatt gcaattaaaa cagtagttgg tttaattgca gcttctaata atgtaaggtc
     2401 tattaaccca tctccattta cttctaaata ggttacctca cctcctactt tttcaatatg
     2461 tttacaagta tctaatactg ctttatgttc agtggtaact gttataatat ggttgccttt
     2521 acttgcatac atttcaaaca cacctttaat ggctaaatta tctgcttcgg tagcaccact
     2581 tgtaaaaatt atttccttag catctgcacc tactaatttt gctacttgct cccttgcata
     2641 atccacagct tcttctgcct cccatccaaa ttggtggttt ctgctggctg cattaccaaa
     2701 atgttgtgta aaaaaaggca gcatagcttc taacactctt ggatccattg gagtggtagc
     2761 attattatct aaataaattg gcaacttcaa catatcttgc atttaaaaaa tttaaaaaaa
     2821 tatgccacaa atgtactatt ttttaggctt ctttttaaga gataacagct ttatgaattt
     2881 caatttccat atatcgtaag tagaataaaa attattaaat attttttatg aaaaaagtag
     2941 aacatattgg tattgcagtt aaagattttg ccattagcat accattattt gaaaatttat
     3001 taaatgtacc ctgttataaa caagaaacag tagaatcaga aaatgttaac actgcatttt
     3061 ttaaacttgg cgaaactaaa attgagcttt tgcaaagtac aaattctgat ggcgtaattg
     3121 caaaatttat tgagaaaaaa ggcgaaggca tccatcactt agcttttgat actgataata
     3181 ttttggcgga agtagaaagg ttaaaaaatg ctggctttac tttgttaaat gaaacaccca
     3241 aagaaggtgc agataataaa ttggtttgtt ttctgcatcc taaaagtact aatggtgttt
     3301 taatagaatt atgccaagaa aaaaaatgat atacctactg tttaaatgat ataaataaaa
     3361 taattattaa gccataatct aattatattt ttaaaatgtt tgcagtaaca attttaggaa
     3421 acaattcagc aatacctgct tatggcagaa tgccaactgc tcaagttgtt actttaaacg
     3481 accaacagtt tttaattgac tgtggcgaag gaacacaaat gcaacttgct aaatataaaa
     3541 taagaaaaag caagattaat catattttca tttctcattt acatggcgac cattactttg
     3601 gtttaattgg tttaattaca caaaaattgc tgaaccattg aatatggccc ata
//
LOCUS       DAOQPK010000218         3649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_17645_length_3649_cov_5.059544, whole genome shotgun sequence.
ACCESSION   DAOQPK010000218 DAOQPK010000000
VERSION     DAOQPK010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17645_length_3649_cov_5.059544"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PLU36_09160"
     CDS             complement(<1..230)
                     /locus_tag="PLU36_09160"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46958.1"
                     /translation="MIACKTNEIEKKTYEWPKDANAPIAEVKPFTRIWHNDTVVDNYY
                     WMIDYFKKGPDSTKVVDYLKTENAYTEAMLHGT"
     gene            complement(391..1713)
                     /locus_tag="PLU36_09165"
     CDS             complement(391..1713)
                     /locus_tag="PLU36_09165"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase, eukaryotic"
                     /protein_id="HMZ46959.1"
                     /translation="MGIIKDTFKSKADIANSEIKAILNEHGNKKIGEVTLAQIYQGMR
                     GITGLVSETSLLDAQEGIRFRGYSIPELQAKLPKAEGGNEPLPEGLFYLMLLGELPTE
                     NDVQHLTSVWQRRSHVPSHVFQTIDALPLTAHPMTMFVTGIMALQTESHFAEEYSKGI
                     NKKDYWNPMYDDAMDLIARLPRIAAYVYRRKYKNNQHIQPNGLLDWSGNLAHMMGYND
                     PRFKELMRLYMTIHADHEGGNVSAHTTHLVGSALSDPYLSYAAGMNGLAGPLHGLANQ
                     EVIKWIFEMQEQLGTDSPTKAQIEQYVKDTISSGKVVPGYGHAVLRKTDPRFSAQMEF
                     GKKHMPDDKLCQTVWNIYETVPPILQSLGKIKNPWPNVDAHSGALLVHFGLVEYEFYT
                     VLFAVSRALGVMASLCWDRALGFPLERPKSVTTESVKKWLEGKDEIWD"
     gene            complement(1908..3005)
                     /locus_tag="PLU36_09170"
     CDS             complement(1908..3005)
                     /locus_tag="PLU36_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HMZ46960.1"
                     /translation="MKKLDWYILKKFFATFFFSILLFAIIAIVIDASEKADDFVQSKL
                     SIKNIITDYYFGFVPHIIALLFPLFVFIAVIFFTSKMAGKSEIIAILASGVSYNKWLK
                     PYFIGGVLLTLFLWIANQNIIPKANVIHASFLTKYVDANSSYEALMSSQRKRDLYFRL
                     DSFSYAGIINYDTSSKSGGQFFLNKFKGTKIVENLRSNEIRWDNEKNKWILTSVLVRK
                     INGLQEEMVEFESKEMNFNFKPFDLRKDTYVKSKLTTEELKRYIQLEEMRGSESINEL
                     KVERYKRDAGAFSVLLLTLIGAIIAGRKVRGGSGMHLAWGFIIAALYIITDKFSTIFS
                     SKGNFQPLIAAWLPNIFFCFVAYFLYRKAPK"
     gene            complement(3194..3322)
                     /locus_tag="PLU36_09175"
     CDS             complement(3194..3322)
                     /locus_tag="PLU36_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46961.1"
                     /translation="MATAKKAAPKKAAPKKAAPKKAAPKKAAPKKAAPKKAAPKKK"
     gene            complement(3392..>3649)
                     /gene="rpmA"
                     /locus_tag="PLU36_09180"
     CDS             complement(3392..>3649)
                     /gene="rpmA"
                     /locus_tag="PLU36_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HMZ46962.1"
                     /translation="KKGEGSVKNGRDSNSKRLGVKIYGGQPATAGNIIVRQRGTTYHP
                     GKNIGVGKDFTLFALADGIVEFRKTKNDKTVVSVSAVEAAV"
BASE COUNT         1145 a          606 c          510 g         1388 t
ORIGIN      
        1 gtgccatgta acattgcttc tgtataagca ttttctgttt ttaaataatc tacaactttt
       61 gtgctatctg gccctttctt aaagtagtca atcatccaat agtaattatc cactactgta
      121 tcgttatgcc aaattctggt aaatggtttt acctctgcaa taggtgcatt tgcatctttg
      181 ggccattcat aagttttctt ttctatttcg tttgttttgc aagctatcat actagttgtt
      241 attagtagaa ttaatatttt cttgtacata aaataaattt ttaagcttat tgcaatgtag
      301 taaaaattaa tttctttagt ttaattatat ataagtggta aaaaaaagaa gctactgtat
      361 aagtagcttc tgaattagga taaaaaaata ttaatcccaa atttcgtctt taccctctaa
      421 ccattttttc acactttcgg tagttacact ttttggtctt tctaaaggaa aacctagtgc
      481 tctatcccaa cataagcttg ccattacacc taatgcacgg cttactgcaa ataaaacggt
      541 ataaaattcg tattctacca aaccaaaatg tactaataaa gctccactat gtgcatctac
      601 atttggccaa gggtttttta tttttcctaa agattgtaaa ataggaggaa cagtttcata
      661 aatattccaa acggtttggc aaagtttatc atcgggcatg tgttttttac caaattccat
      721 ttgtgctgaa aaacgtgggt ctgtttttct aagtactgca tggccataac ctggtacaac
      781 tttacctgaa gagatggtat ctttcacata ttgttctatt tgtgctttgg ttgggctatc
      841 tgttcctaat tgttcttgca tttcaaaaat ccatttaatt acttcttgat tagctaagcc
      901 atgtaaaggg ccagctaaac cattcatacc agctgcataa cttaaataag gatcgcttaa
      961 tgcactacca actaaatggg ttgtatgtgc agatacatta cctccttcat gatctgcatg
     1021 tatagtcatg tataaacgca ttaactcttt aaatcttgga tcgttataac ccatcatgtg
     1081 tgcaagatta ccagaccaat ctaataaacc atttggttgt atatgttgat tgttcttata
     1141 ttttcttctg taaacgtaag ctgcaatacg tggtaatcga gcaattaaat ccattgcatc
     1201 atcatacatt ggattccaat aatctttttt attgatacct tttgaatact cttcagcaaa
     1261 atgactttct gtttgtaaag ccataatacc tgttacaaac atagtcattg gatgagctgt
     1321 tagtggtaat gcatcaattg tttgaaaaac atgtgatggt acatgactac ggcgttgcca
     1381 tacactagtt aaatgttgta catcattttc tgtaggtaat tctcctaata acattaaata
     1441 aaacaatcct tctggtaatg gttcatttcc tccttctgct ttagggagtt ttgcttgtag
     1501 ttctggtata gaataacctc taaacctaat tccttcttgt gcatctaata aacttgtttc
     1561 agaaaccagg cctgttatac cacgcatacc ttgatagatt tgagcaaggg taacttctcc
     1621 aattttcttg ttaccatgtt catttaatat tgcttttatt tcgctattgg caatatctgc
     1681 tttgctttta aatgtgtcct taataattcc catttcttta ttttttttag tttattatca
     1741 agaataaacc ttgttatata atataactat aatcaaaaaa gattataatt ttcaatttgt
     1801 ttaataggga tttttttaaa taatgctaca ttatgcaatg tatattattg aaagtggtta
     1861 aagataaagt aagtaatttg tactacaaag tacagtagaa gaaaaattta ttttggtgct
     1921 tttctatata aaaagtaagc aacaaaacaa aagaaaatat taggtagcca agctgcaata
     1981 agtggttgaa aatttccttt tgaggaaaag atggtagaaa atttatcagt aattatatac
     2041 aaagcagcaa taataaagcc ccaagctagg tgcataccac taccgcctct tacttttctt
     2101 ccagcaataa ttgccccaat taaagttaat aaaagcacag aaaaagcacc tgcatcacgt
     2161 ttgtatcttt ctacttttaa ttcatttata gattcggagc ccctcatttc ttctaattga
     2221 atatatcttt taagttcctc ggtggttaat ttacttttta cataagtatc ttttcttaaa
     2281 tcaaaaggtt taaaattaaa attcatttct tttgattcaa attctaccat ttcttcctgt
     2341 aatccattta tttttcttac caatacagaa gttagtatcc atttgttttt ttcattatcc
     2401 catcttattt cattgcttct taagttttca actattttag ttcctttaaa tttgtttaaa
     2461 aaaaactgac ctcctgattt actgctagta tcgtagttaa taatgccagc ataagaaaaa
     2521 gaatctaatc taaagtataa atctctttta cgttggctac tcattaatgc ttcgtaagat
     2581 gagtttgcat ctacatattt agttaaaaaa cttgcatgaa taacatttgc ttttggaatg
     2641 atattttgat tagctatcca taaaaaaagg gtaagcaaca caccaccaat aaaatatggt
     2701 tttaaccatt tgttgtagga tacaccgctt gcaagtattg caataatttc actttttcct
     2761 gccatttttg aagtaaaaaa aataactgca ataaatacaa aaagagggaa gagtaatgcg
     2821 attatatgtg gtacaaaacc aaaataataa tcagtaatta tatttttaat acttagtttg
     2881 gattgtacaa agtcgtctgc tttttcacta gcatcaataa caattgctat gatagcaaac
     2941 aataaaattg aaaaaaagaa tgtagcaaag aattttttta atatatacca atcaagcttt
     3001 ttcatacttt aattacactt ggcaaatata cttgcatatt actatcaata ggttaaagat
     3061 gaataatctt acttaggtta ttttatttaa cttgtacatg gcaatttttt gtttaaaagc
     3121 cttgctattg cttatttaca ttaaaataaa aactccgata tatatcggag tttttatttt
     3181 cttatagaag aaattatttc ttcttaggag ctgctttttt aggagctgcc tttttaggag
     3241 ctgctttttt tggagctgct ttttttggag ccgctttttt tggtgctgct ttttttggtg
     3301 cagctttttt tgcagtagcc attttgtttt aaatttaatg gttagttaat gccataaaat
     3361 tataaacatt ttttaaactg caaaattttt tttatacagc agcttcaaca gccgaaactg
     3421 aaactacagt tttatcattt tttgtttttc taaattcaac tattccgtct gctaatgcaa
     3481 ataatgtaaa gtcttttcct acaccaatat ttttaccagg atgataagtt gttcctcttt
     3541 gacgaacaat tatattacct gctgttgctg gttgaccacc atatattttt acacccaaac
     3601 gtttgctgtt agaatcacgt ccgtttttta cactaccttc ccctttttt
//
LOCUS       DAOQPK010000219         3619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_17926_length_3619_cov_4.952581, whole genome shotgun sequence.
ACCESSION   DAOQPK010000219 DAOQPK010000000
VERSION     DAOQPK010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17926_length_3619_cov_4.952581"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            552..1322
                     /locus_tag="PLU36_09185"
     CDS             552..1322
                     /locus_tag="PLU36_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HMZ46963.1"
                     /translation="MIIDTHCHLYLDEFEKDRLEVMQRAIKKGVQKFYLPSIDSTVID
                     KMLRMEKEYPKNCFLMMGLHPCSVNENIDAELTTVLNCLKQRPFAAVGEIGLDFYWDT
                     SFKEQQYNAFKTQMQWAIDYNLPIVIHSRNAMQETINTVKPFAAMGLRGIFHCFSGSI
                     EEANTIINMNFLLGIGGVITYKKSGLAETLKEIALSNVVVETDAPYLTPIPFRGKRNE
                     SAYLEYIISEIAKVKKCSVQEVITTTTNNAENLFNKYE"
     gene            complement(1559..2974)
                     /locus_tag="PLU36_09190"
     CDS             complement(1559..2974)
                     /locus_tag="PLU36_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ46964.1"
                     /translation="MLIKQLYTGCLSEAAYFIANNGEAAVIDPLRDIDEYLQLAKEHK
                     VSIKYIFETHFHADFVSGHLDLAAATGATIVYGPNTDTKLKVHIAFDGEVFKIGNLSV
                     KVLHTPGHTLESTCFLLKDEQEKDYAVFTGDTLFVGDVGRPDLAQKGADLTMNDLAGM
                     LYESLQNKIFPLDNQVIVYPAHGAGSSCGKNLGKETYSTIGEQKISNYALQTQTKEDF
                     VKAVTEGLLAPPQYFPINAKINKEGYQSISTILENGLKALSVNEFKAKASDENVIIVD
                     TRNATVFTNGFVPNSISIGLEGRFAEWAGSILPFTKSILLVTEKGKEKESVVRLSRVG
                     FDKFAGYLDGGFEAWQLANENTDMIVDVECDELAMDIPFDENLIVVDVRRETEYADGH
                     LKQSINIPLNELTDPASMANLEDNHNIYIHCAGGYRSVIAASLLKQQGIHNLRNVLGG
                     WSKIKELADKFEIEKSTEVLN"
     gene            3079..3525
                     /locus_tag="PLU36_09195"
     CDS             3079..3525
                     /locus_tag="PLU36_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HMZ46965.1"
                     /translation="MSIITAERIAADFEMQMTDATGNTMKMDIPIEQGGNGKGLRPMQ
                     TMLAALCGCSTVDIVSILNKQKQTIETFKVFVDGEREKNKTMALWQTIHLVFEMSGNI
                     EPDKAFRAVQLSLDKYCSVAETLRKAGASISFTVILNNEKLNYQPE"
BASE COUNT         1331 a          567 c          537 g         1184 t
ORIGIN      
        1 ttcctgatcc tgaagatgat atacctgtag atggtggttt aactttattg ttagcagcag
       61 gagctggttt aggagcaaag aaattaagag ataacagaaa aaaacaacag aataaaaact
      121 aaacagcttg ttttagaact attacaaatg tgtaatttta attaattaca ttttagccta
      181 attaaataac aacaaaaagt ataaaagctc ttgtaactat gcaagggctt ttttatagag
      241 taacatgaaa taaaatgagc cgaggtagaa acctcagctc ttatttaatc cgtaccctat
      301 gaaaacaagt ttatataaat ttagctatta catcaagcta aaaattaatc cataatttta
      361 atgtattttg ctaaaaattc agtgcagcat ttgtggaatt acaatacaac atgtaagaat
      421 gtacattggc aacattggat tttaatttaa acaaaattgg tttttataat tacttatatt
      481 ggtagaaaaa agtaattact ctataaaaac gaattagtat aaattttagt atataactta
      541 aaaaaaaata catgataatt gatacccact gccatttata tttagatgaa tttgaaaaag
      601 acaggttaga ggttatgcaa agggctataa aaaaaggggt acaaaaattt tatttaccaa
      661 gcatagatag tacagtaata gataaaatgc tgaggatgga aaaagagtac cctaaaaatt
      721 gttttttaat gatgggctta catccttgct cggtaaacga aaatatagat gccgaattaa
      781 caactgtttt aaactgttta aaacagagac catttgcagc agttggtgaa attggtttag
      841 acttttattg ggatacaagt tttaaagagc agcagtataa tgcatttaaa actcaaatgc
      901 aatgggctat agattataat ttgccaattg taatacatag tagaaatgcc atgcaagaaa
      961 ccattaacac tgtaaagcct tttgctgcta tggggttgag aggaatattt cattgttttt
     1021 ctggaagcat tgaggaggct aatacaatta taaatatgaa ttttttatta ggtattggag
     1081 gggtaataac ctataaaaaa tctggtttag cagaaacctt aaaagaaata gcattaagta
     1141 atgtagtggt tgaaacagat gctccttatt taacacctat accttttagg ggaaagagga
     1201 atgaaagtgc ctatttagag tatattattt ctgaaattgc aaaagtaaaa aaatgctcag
     1261 tgcaagaggt tataaccacc accacaaaca atgcagaaaa tttgtttaac aagtatgaat
     1321 agttatagat ttatgtttaa aggtttactc caaatttttt ttattaaaaa cagcatcaaa
     1381 acagtactaa caacaagcca tatataaaat aaaatattac ctaatgaaaa atgattctta
     1441 aaaaaagcaa actgaaattt aataccaaat gtaatattag aaagtaacca aacaattaag
     1501 gtagaaagtg tataaacaat tctatttagc atttttctta attcaggttc cataaaaatt
     1561 agtttaacac ttctgtactt ttttcaattt caaatttatc tgctaactct tttattttac
     1621 tccaccctcc taaaacattt cttaaattat gaattccttg ttgctttaat aaagaggctg
     1681 caattacact tctgtaacca cctgcacaat gaatataaat attatggtta tcttctaaat
     1741 tagccatact tgcaggatcg gtaagttcat ttaaaggaat attaatagat tgctttaaat
     1801 gaccatcagc atattctgtc tctctcctaa catctactac aattaaattt tcatcaaaag
     1861 gaatgtccat tgccaattca tcacactcaa catctacaat catatctgta ttttcgttgg
     1921 caagttgcca tgcttcaaac ccaccatcta aatagcctgc aaatttatca aatccgactc
     1981 tcgataatct tacaacactt tctttttcct tccctttttc tgtaactaat aaaatagatt
     2041 tagtaaaagg taaaatacta ccagcccatt ctgcaaatct gccttctaac cctatagaaa
     2101 tactattggg cacaaaacca ttggtaaaaa ctgttgcatt acgagtatct acaataatta
     2161 cattttcatc acttgcttta gctttaaatt catttactga taaagctttt aatccatttt
     2221 ctaaaattgt acttatactc tggtagcctt ctttatttat tttagcatta ataggaaaat
     2281 attgaggtgg agcaagcaac ccttcggtta ctgctttcac aaaatcttct tttgtttgag
     2341 tttgtagagc ataattagaa attttttgct ctccaatagt actatatgtt tctttaccta
     2401 aatttttacc acaactgctt cctgcaccat gagcaggata aacaattacc tgattatcta
     2461 agggaaaaat tttattttgt aagctctcgt acaacatacc tgccaaatca ttcatagtta
     2521 aatctgcccc tttttgagct aaatctggcc ttcctacatc gcctacaaaa agtgtatcac
     2581 ctgtaaaaac tgcataatct ttttcctgtt catcttttaa taaaaagcaa gtactttcta
     2641 aagtgtgacc aggagtatgt aatactttta cagataggtt accaatttta aaaacctctc
     2701 cgtcaaaagc aatatgcact ttcagtttag tatcggtatt agggccatat acaatggttg
     2761 cacctgttgc tgctgcaagg tctaaatgcc cacttacaaa atcagcatga aaatgggttt
     2821 caaaaatata tttaatacta accttgtgtt ccttggctaa ttgtaaatat tcatcaatat
     2881 ctcttaaggg gtctatcact gctgcttctc cattatttgc aataaaataa gcagcttcgc
     2941 ttaaacagcc tgtatataat tgtttaatta gcatctataa taattttttc aaagatacca
     3001 tataaaaata tattctacat tcttacacca taattgtcat ttaaaaaaca cttaacttag
     3061 aagtaaaaat tatattttat gtcaattata actgccgaaa gaattgctgc agattttgaa
     3121 atgcaaatga ccgatgcaac aggtaacact atgaaaatgg atatacccat tgaacaaggt
     3181 ggaaatggaa aagggctaag gcccatgcaa actatgcttg cagcactttg tgggtgtagc
     3241 actgtagata ttgtaagtat attaaataag caaaaacaaa ctattgaaac atttaaagtt
     3301 tttgtagatg gcgaaaggga aaaaaataaa accatggctt tgtggcaaac cattcattta
     3361 gtttttgaaa tgagtggcaa tattgaacca gataaagcat ttagagctgt tcaattatcg
     3421 ttagataaat attgctcggt tgcagaaact ttaagaaaag ctggtgccag cattagcttt
     3481 acggttatat taaataatga aaaattaaat taccaaccag aataaaatga agaagaaaac
     3541 tttagtttta ggtgcttctg ataacacaga aagatacagc aatatggcag taaaaaagtt
     3601 agttgctcat ggacatact
//
LOCUS       DAOQPK010000220         3610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_18000_length_3610_cov_4.740084, whole genome shotgun sequence.
ACCESSION   DAOQPK010000220 DAOQPK010000000
VERSION     DAOQPK010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18000_length_3610_cov_4.740084"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="PLU36_09200"
     CDS             <1..1006
                     /locus_tag="PLU36_09200"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HMZ46966.1"
                     /translation="NHQKIFNPGLPEVRNYVTKIVEDIVARYNIDAIHMDDYFYPYPD
                     KNNNEFNDKESFEMYGNGLKKEDWRRSNCDSIIKQLHNAIKSIKPYVKFGISPFGVWR
                     NFSRDSLGSNTKAGISNYDDLYADILLWLKEGWIDYVTPQLYWERGHKLCDYDTLLNW
                     WNKYTYNKHLYIGHGIYRAGTNAGWRNKNELPTQIKHLRQYSTTQGSIFFSSKSFENN
                     PNGWNDSLKNNYYAYAAIPPPMPWIDSIMPIMPLVEKTTNNHFYVTIANNTNIKGFAV
                     FSIDEQKEPYLENARIEQYIVLGDSNKIEPDKWFEQKRRIFVSSVSRGNNISEWVELK
                     "
     gene            complement(1019..1366)
                     /locus_tag="PLU36_09205"
     CDS             complement(1019..1366)
                     /locus_tag="PLU36_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46967.1"
                     /translation="MKYHVPYNFILNYLYPLRPTIKKMLGGYTLQLNKKIFFFLRESD
                     TSPEFNGVFVACIAEFFEELQAEIHTSKMEFDLDGSINSWIFISEDLPDFEQKVLIAC
                     NLIKDGDVRMGKI"
     gene            complement(1373..1882)
                     /locus_tag="PLU36_09210"
     CDS             complement(1373..1882)
                     /locus_tag="PLU36_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46968.1"
                     /translation="MLTKATIEKFFIAEKQIGFFLLIVGLIALTIAVYALFYLKLQEL
                     KWPTIIICLLSIVMLFVGYQQVANATKSRISIIYAFDMNPELIKTQELPRVEKKIITI
                     QVLKYISVLVFLAMLWFVFFKKDKLLNSTSNAVLISFALLFLLFFSMFIFQEISTKKY
                     AQQIKSFVS"
     gene            2040..3527
                     /gene="rpoN"
                     /locus_tag="PLU36_09215"
     CDS             2040..3527
                     /gene="rpoN"
                     /locus_tag="PLU36_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HMZ46969.1"
                     /translation="MSLSQSLQQKLLQKLSPQQIQLMKLLQVPTANLEDRIKEELEEN
                     PALEQVDDAYDNDYNDEFKDEFESNNEEEVLDGSEDEYENIDISEYVSDSDDDIADYK
                     TRDENYGDTEENKIIPIKNEQSFYELLVNQLGLLQLDDRMYKVAEQIVGSLDDDGYLR
                     RELSSIVDDLAFRQNIITNEQEVAELITQIQQFDPPGIAAKDLQECLLLQLKREITED
                     DYVKIAIKILQKYFDEFTKKHFDKIQKGLGINEVELKEAIQQIVKLNPKPGGNIGEIN
                     KAENYIVPDFFILNNAGILELTLNNKNAPDLRISEGYRDMLKDYEKGSKKDKKQKEAV
                     LFIKQKIDSAKWFIEMIQQRQRTLFNTMYAIMKHQENFFLTGDETELKPMILKDIAEV
                     TLQDISTVSRVANSKFVQTEFGNYRLKFFFSESLSTDSGEEVSTREVKKILSDIINAE
                     DKNKPLADEVLTDLLNEKGYNIARRTVAKYREQLNIPVARLRKEL"
BASE COUNT         1478 a          472 c          588 g         1072 t
ORIGIN      
        1 taatcatcaa aaaattttta atcctgggct acccgaagtt agaaattatg taaccaaaat
       61 tgtagaagat attgtggcaa gatacaatat tgatgctata cacatggatg attactttta
      121 tccttatccc gataaaaaca ataacgaatt taacgataaa gagagttttg aaatgtatgg
      181 aaatggttta aaaaaagaag attggagaag gagcaattgt gatagtataa ttaaacaatt
      241 acataatgca attaagtcta ttaaacctta tgtaaaattt ggtattagcc cttttggcgt
      301 ttggagaaat tttagtagag atagtttagg tagcaacact aaagcaggaa ttagtaatta
      361 cgatgatttg tatgccgata ttttactttg gttaaaagaa ggttggatag attatgttac
      421 accacaactt tattgggaga gagggcataa actttgcgac tatgacacct tgttaaattg
      481 gtggaacaaa tacacctata ataagcattt gtatatagga catggtatat atagagcagg
      541 taccaatgca ggatggagaa ataaaaacga gttgccaaca caaataaaac atttaaggca
      601 gtatagcact acacaaggaa gtattttttt tagtagtaaa agttttgaaa acaatcctaa
      661 tggctggaat gattctttaa aaaataatta ctatgcttat gcagcaatac cacctcccat
      721 gccttggata gatagtataa tgcctattat gccattggta gaaaaaacaa ctaataacca
      781 cttttatgtt acaattgcaa acaataccaa cataaaaggg tttgccgtat ttagtatcga
      841 tgagcagaaa gagccctatt tagaaaatgc aagaatagag cagtatattg ttttaggaga
      901 ctctaataaa attgaacctg acaaatggtt tgagcaaaag agaagaatat ttgtaagctc
      961 tgttagtcgt ggcaataata ttagtgagtg ggttgaatta aaatagtttt aaaagaagtt
     1021 aaattttccc cattcttaca tcaccatctt ttataagatt acatgcaatt aaaactttct
     1081 gttcaaaatc gggtaaatct tcactaataa aaatccagct atttatacta ccatctaaat
     1141 caaactccat tttactggta tgaatttctg cttgcagttc ttcaaaaaac tctgcaatac
     1201 atgcaacaaa aacaccatta aactcagggc ttgtatctga ctctcttaag aaaaaaaata
     1261 ttttcttatt aagctgtaat gtatatccac caagcatttt tttaatggtt ggcctcaaag
     1321 ggtataaata gtttaaaata aaattatagg gaacatggta tttcataaat tattaactaa
     1381 caaaagattt aatttgttga gcatattttt tggtgcttat ttcttgaaaa ataaacatgg
     1441 aaaaaaacaa tagaaaaagt aaagcaaagc taataagaac agcattagaa gttgagttta
     1501 gtagtttatc ttttttaaaa aaaacaaacc acaacatagc caagaaaact aaaacagaaa
     1561 tatactttaa tacctgaata gttattattt ttttttcaac tcttggcaat tcttgtgttt
     1621 taattaattc aggattcata tcaaaagcat aaataatact aattctactt ttagtagcat
     1681 tggcaacttg ttgatagcct acaaatagca taacaatact tagtaggcaa attataatgg
     1741 taggccattt caattcttgt aacttcaaat aaaataaggc atatactgca attgttaaag
     1801 caataagacc aacaattaat aaaaaaaatc caatttgttt ttctgcaatg aaaaattttt
     1861 caatagttgc tttagtaagc attagtaaat aattttaaag tgtgaagata aactttattt
     1921 gaaaggacaa ttaaaccatt ttttttcaaa aaaacaatta caaaaaatgg ctttcatagt
     1981 atagtttaca attaacttta cagccaatga aaaggatata tttataaaag ttatttacaa
     2041 tgagtttaag ccagtcttta cagcaaaaat tattacaaaa attatcccct caacaaattc
     2101 agttaatgaa attgttgcaa gtgcctactg caaatttaga agatagaatt aaagaagagt
     2161 tagaagaaaa tcctgcttta gaacaagttg atgatgcata tgataacgac tataacgatg
     2221 aatttaaaga tgaatttgaa agcaataatg aggaggaagt tttagatggc agcgaagatg
     2281 aatatgaaaa catagacatt agtgaatatg taagtgatag cgatgatgat attgcagatt
     2341 ataaaacaag agatgaaaac tatggcgaca cagaagaaaa taaaataata ccaattaaga
     2401 acgaacaaag cttttatgaa ttattagtta atcaacttgg cttgttacaa ttggatgata
     2461 gaatgtataa agtggcagaa caaattgtag gcagtttaga tgatgatgga tacttaagaa
     2521 gagagttaag cagcattgta gatgaccttg cttttagaca aaacataata acaaatgaac
     2581 aagaagttgc tgaactaata acccaaatac aacagtttga ccccccagga attgctgcaa
     2641 aagatttaca agaatgtttg ttattgcaat taaaaaggga aataaccgaa gatgactatg
     2701 taaaaattgc catcaaaatt ttacaaaaat attttgatga atttactaaa aagcatttcg
     2761 ataaaattca aaaagggtta ggcattaatg aagtggaact taaagaagct attcaacaaa
     2821 ttgtaaaact aaatccaaaa ccaggtggca atattggcga aataaacaaa gcagaaaatt
     2881 atatagtacc agactttttt attttgaata atgctggcat tttagaactt accttaaaca
     2941 ataaaaatgc accagattta agaattagtg aaggttacag agatatgctg aaggactatg
     3001 aaaaaggaag taaaaaagat aaaaaacaaa aagaagcagt attatttatt aaacaaaaaa
     3061 tagatagtgc aaaatggttt attgaaatga ttcagcaaag gcaaagaaca ttatttaaca
     3121 ccatgtatgc cattatgaaa catcaagaaa acttttttct tacaggcgat gaaacagaat
     3181 taaaaccaat gattttaaaa gatattgcag aagtaacctt acaagatatt agcactgtaa
     3241 gccgtgttgc caatagcaag tttgtacaaa cagaatttgg taattacaga ttaaaatttt
     3301 tctttagcga aagtctaagt acagattctg gtgaagaagt aagtacaaga gaagttaaaa
     3361 aaatattgag cgatattatt aatgccgaag ataaaaataa acctttggca gacgaagttc
     3421 ttacagactt gttgaacgag aaaggctata atattgcaag aagaacagtg gcaaaatacc
     3481 gagaacaatt aaacataccc gtagctagat taagaaaaga attataaaat tttttaccgt
     3541 ggcaattcag caacaaacct atcaacaagc aaacaatagt attgtactta tagtagcaaa
     3601 agcaatcagc
//
LOCUS       DAOQPK010000221         3586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_18181_length_3586_cov_4.641461, whole genome shotgun sequence.
ACCESSION   DAOQPK010000221 DAOQPK010000000
VERSION     DAOQPK010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18181_length_3586_cov_4.641461"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="PLU36_09220"
     CDS             complement(<1..577)
                     /locus_tag="PLU36_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ46970.1"
                     /translation="MSIKVAGITKIYGTQKAVNNINFNINKGEIVGFLGPNGAGKSTT
                     MKILTGYLAATVGNAFINEINVQENPILTKKQIGYLPESNALYLDMYVKEYLKFIAQI
                     HQLTNSAKAVLHVIELTGLQIEQNKKIGQLSKGYKQRVGLAAALIHNPQVLILDEPTS
                     GLDPNQIIEIRNVIKEQGKNKTVLFSSHILQE"
     gene            complement(588..887)
                     /locus_tag="PLU36_09225"
     CDS             complement(588..887)
                     /locus_tag="PLU36_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46971.1"
                     /translation="MFYSMLLKLISQYYIYLWLLMAVVIGIKILLTFFFNKNVEGFQG
                     LLYSIFKWYNQDDMDMAEGNKRKKLMSFYNLLTVAMYFMLGLIILFSILPMFLTV"
     gene            1048..>3586
                     /locus_tag="PLU36_09230"
     CDS             1048..>3586
                     /locus_tag="PLU36_09230"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ46972.1"
                     /translation="MKILLFYIKRKYTCYIVSIFFTIFSTPLFYSTTQAQTFAELNKL
                     FISLYDNGKYDSAVLIGEKALIQCEKEYGKKDINYAISYLNIADAYAALKKISEAVLN
                     YSKSIEVFAIVYNTYENIDIALIDNKIGSQFYSIQLYDTATTYFLKSIQFFYKHPNNN
                     YESLYNISLNIIECCIQTANYNTLLLTANQALPFVEANNSKESEAYYTMFYYKGFALY
                     NLEQYDKAEYIYKNILPIAEKLYGKNNSEYITVLKYYFRILSKLEKWNKAEETLSEIL
                     QIQERIGYEDVQDVAELYEEAGNFYASIGNYSKAENCFKLALNTLENAGKKNSDLYYS
                     ILHSKAYVIYQAGKLIETKDILENLLKVYTKKNGRVNATNAEILIVLAGAEIQLNYLA
                     AAKEHLDEGYNMTIKVHGKEYPTIANAKESYGLWYNKTGDSKKSIENLKDGIQLLEKI
                     QTTNYNIQYTRLQASLHSNIGITYLEMGKYADAENHLTQSLQIREKILGIKHPEYALS
                     LINLGVVHLYQARYEEADKMLLAALKIYLDNNLTNTVNFYYLINNIALLAEKTNGANQ
                     AKVLYQNILTILEKNNSTNSAVYSIIYGNLATLSFNKEMYEDALHYCTKAIHYLEQGN
                     NQNSKEYIKILNTQFLAYKNKKEFGMAAKMSNPLIELTKKIMGNDAELTGIVYNNIAM
                     LYEELNDVEKAADFLNKGNGILLNNFKQNFYILSEKEKLTWWNNQSFIFNIFPSMLMQ
                     FNITEGKWVEAFANRQIQIKGFVLSDSKNTLNVARNNKNKEVKNYLDKWQYNKDLLSK
                     LYSQPLQDRIYSTDSLEQKANTLEKKINQLSAGNVQLNHKTYTWQNVQ"
BASE COUNT         1421 a          499 c          476 g         1190 t
ORIGIN      
        1 cttcttgtaa gatatggctt gaaaataaaa cagttttatt ctttccttgt tctttaatta
       61 catttcttat ttcaattatt tgattaggat ctaagccact cgttggttca tctaaaatta
      121 atacttgtgg gttatgaatt aatgctgcag ccaaacctac acgttgttta taacctttag
      181 ataattggcc aattttttta ttttgttcaa tctgtaaccc agttaattca attacatgta
      241 aaacagcctt agcagaattt gttaactgat gaatttgagc aataaatttt aaatactctt
      301 ttacatacat atctaaataa agagcattgc tttcgggtaa ataaccaatt tgttttttag
      361 ttagtattgg gttttcctgc acattaattt cattaataaa tgcattacca acagttgcag
      421 ctaaataacc tgttaatatt ttcattgtag tgcttttacc ggcaccatta ggacctaaaa
      481 atcctacaat ttctccctta ttaatattaa aatttatatt atttactgct ttttgagttc
      541 cataaatttt tgtaattcca gcaaccttaa ttgacattta aaataattta tacagttaaa
      601 aacattggta aaattgaaaa caaaataata agacctaaca taaaatacat tgctacagta
      661 agtaaattat aaaagctcat caacttctta cgtttattcc cttctgccat atccatatca
      721 tcttgattat accatttaaa tatagagtaa agcaagcctt gaaatccttc tacattttta
      781 ttaaagaaaa aagtaagcaa tattttaata ccaattacta cagccattaa aagccataaa
      841 taaatataat attgactaat tagttttaat aacatagaat aaaacacata actgattatt
      901 gttagaacaa gttcatttca ttgttttaca acaatcattt aagctactaa attgtagaaa
      961 atttaaacaa ctgcaaaaaa gcattttttt atctttaaaa tcaagaaaat taagcttatt
     1021 tacataagtt ttgcattaac tttatgtatg aaaattttat tattctacat aaagagaaaa
     1081 tatacttgtt atattgtttc aattttcttc accattttct ctactccttt attttatagc
     1141 acaacacaag ctcaaacttt tgctgaattg aacaaattgt ttatttcatt atatgataac
     1201 ggtaaatacg attctgctgt attgattgga gaaaaagcat taattcaatg cgaaaaagaa
     1261 tatggtaaaa aagatattaa ctacgccatc tcatatctaa acattgctga tgcctatgct
     1321 gcattaaaaa aaatatctga agcggtatta aactatagta aatctattga agtatttgcc
     1381 attgtatata acacctacga aaacatagat attgcactga ttgataataa aattgggtct
     1441 caattttatt ctattcaatt atatgataca gctactactt attttttaaa gagtattcaa
     1501 tttttttata aacaccccaa taataattat gaaagccttt acaatattag tttaaacatt
     1561 atagaatgct gcatacaaac agctaactat aatactttat tacttactgc caatcaagct
     1621 ctaccttttg ttgaagccaa taattctaaa gaatcggaag cttattatac tatgttttat
     1681 tataaaggct ttgccttata taatttagaa cagtatgata aggcagaata catttataaa
     1741 aatattttgc ctatagcaga aaaattgtat ggaaaaaaca attcggaata tataacagtt
     1801 ttaaagtact attttagaat attaagcaaa ttagaaaaat ggaataaagc cgaagaaacc
     1861 ttaagtgaaa tattacaaat tcaggaaaga attgggtatg aagatgtgca agatgtagct
     1921 gaactatatg aagaggcagg taatttttat gcatccatag gcaattattc aaaagcagaa
     1981 aactgtttta aattagcatt aaatacattg gaaaatgcag gaaagaaaaa cagtgattta
     2041 tattactcta tactacacag taaagcttat gttatttatc aagctggtaa actaattgaa
     2101 actaaagata tacttgaaaa cttgttaaaa gtttatacta aaaaaaatgg cagagtaaat
     2161 gcaacaaatg cagaaatttt aattgtttta gcaggtgccg aaatacaatt aaattatttg
     2221 gcagccgcca aagaacattt agatgaaggc tataatatga ctataaaagt acatggtaaa
     2281 gaatatccaa ccattgcaaa tgcaaaagaa agttacgggc tatggtacaa caaaacaggt
     2341 gatagtaaaa aaagtataga gaatttaaaa gatggtattc aacttctaga aaaaattcaa
     2401 acaaccaatt acaatattca atatactaga ctacaagcat ctttacactc taatattggt
     2461 ataacatatt tagaaatggg taaatatgca gatgctgaaa atcatttaac ccaatcgtta
     2521 caaattagag aaaagatttt aggtattaaa catccagaat atgcattaag tttaataaac
     2581 ttaggcgttg ttcacttata tcaggcacgg tacgaagaag cagataaaat gttactggct
     2641 gctcttaaaa tttatttaga taataactta acaaatactg taaattttta ttacttaatc
     2701 aacaatattg cattattggc agaaaaaaca aatggtgcca atcaagctaa agttttatat
     2761 caaaacatac ttacaattct ggagaaaaac aacagtacaa attctgctgt ttatagtatt
     2821 atatatggca atttggcaac cttaagtttt aataaggaaa tgtatgaaga tgctttacat
     2881 tattgtacta aagctattca ttatttagag caaggtaata accaaaattc aaaagagtat
     2941 ataaaaatat taaataccca atttttagca tataaaaata aaaaagaatt tggcatggct
     3001 gctaaaatga gtaacccact tatagagtta actaaaaaaa ttatgggtaa tgatgcagag
     3061 ctaactggca ttgtttataa taatattgct atgctgtacg aagaactaaa tgatgttgaa
     3121 aaagcagccg attttttaaa caaaggcaat ggtattttat tgaataattt taagcaaaat
     3181 ttttatatac ttagcgaaaa agaaaagctt acctggtgga ataatcagtc ttttattttt
     3241 aatatttttc catctatgtt aatgcagttc aatattactg aaggcaagtg ggtggaggct
     3301 tttgcaaata ggcaaattca aataaaaggc tttgtattat ccgattctaa aaacacttta
     3361 aatgtagcta gaaataacaa aaacaaagaa gtaaaaaact atttagacaa atggcagtac
     3421 aataaagacc tactatcaaa attatattct caaccattac aggatagaat atattccaca
     3481 gatagtttag agcaaaaagc caatacactt gagaaaaaaa taaaccaact tagtgcaggc
     3541 aacgtacagc ttaaccataa aacttataca tggcaaaatg tgcaac
//
LOCUS       DAOQPK010000222         3561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_18404_length_3561_cov_4.673132, whole genome shotgun sequence.
ACCESSION   DAOQPK010000222 DAOQPK010000000
VERSION     DAOQPK010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18404_length_3561_cov_4.673132"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PLU36_09235"
     CDS             <1..278
                     /locus_tag="PLU36_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46973.1"
                     /translation="AVIFEGTCRGCAFESTTSFKIYDSLSLIKLLSIKTIDNNPPDMD
                     GGNISKEVELVPLQTGNTTIKVYKIFGQETEKEDTMHATLYKIKITN"
     gene            333..650
                     /gene="paaB"
                     /locus_tag="PLU36_09240"
     CDS             333..650
                     /gene="paaB"
                     /locus_tag="PLU36_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HMZ46974.1"
                     /translation="MEAGSNASNNNKNIAWPLWEVFIRSKQGLDHKHVGSLHAADEQM
                     AIENARDVYTRRNEGVSIWVVESKYITASNPEEAGELYEPAVNKIYRHPTFYKLPDEV
                     DYM"
     gene            707..1444
                     /gene="paaC"
                     /locus_tag="PLU36_09245"
     CDS             707..1444
                     /gene="paaC"
                     /locus_tag="PLU36_09245"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HMZ46975.1"
                     /translation="MQAAKENFLLYLADSTHILSHRNSEWCGHAPILEQDIALTNIAL
                     DLLGQSRNFYQYAAELIGNNATEDSLAYLRIESEYKNLLLVELPKEDWAHTVLRQFYF
                     SVFLFYLYQQLQQNKDVQIAAIATKAIKEVAYHVRWSGEWVIRLGDGTEESNQRMQYA
                     VEAYKNFTGEFFVLTNYEKQMQIDFELIQQQWNDKVAAVFNDATLSFTNSNFHQVGGK
                     EGRHTEYLGYILTDMQYLQRTYPNATW"
     gene            1438..1941
                     /gene="paaJ"
                     /locus_tag="PLU36_09250"
     CDS             1438..1941
                     /gene="paaJ"
                     /locus_tag="PLU36_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HMZ46976.1"
                     /translation="MVNEQSISVANIWKIVETIPDPEIPVISIVELGVVREVKIENDI
                     IEIIITPTYIGCPAMDMITMHIKLALMEAGFKNVKVTSVLSPAWTTDWMTEETKNKLK
                     AYGIAPPNTKQIVCTPDLFIQDEAVACPHCNNFNTKMVSRFGSTACKALYFCNNCNEP
                     FDYFKCH"
     gene            1980..2756
                     /locus_tag="PLU36_09255"
     CDS             1980..2756
                     /locus_tag="PLU36_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HMZ46977.1"
                     /translation="MPTVLFEIKNNIGFITLNRPEKFNAFNREMALLLQDILTTCNNN
                     NQVRAVFITGAGKAFCSGQDLAEVVDPNGPGMNRILAEHYNPIIQKIRSLEKPVIAAV
                     NGVAAGAGANIALCCDIVIAAENATFIQAFSKIGLIPDSGGTYFLPRLIGWQKASAIM
                     FLGDKITAVEADKWGMIYKFFSADVFETEALKIANTLAEMPTKGLYFTKQALSNSFTN
                     TLHEQLVDEDKLQQQAAATFDFKEGVAAFLEKRKPIFKGE"
     gene            2806..3222
                     /gene="paaI"
                     /locus_tag="PLU36_09260"
     CDS             2806..3222
                     /gene="paaI"
                     /locus_tag="PLU36_09260"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HMZ46978.1"
                     /translation="MTTTPQQVVAHMMQHDLFSQWLGIEVLEIKEGYSKIKMLVRPEM
                     ANGFNIVHGGIAFSLADSAFAFACNNRNNLSVALDTSINFTKPVHVGDILIAEAKELH
                     NGKSTGLYHITITNQNNHIVAMFKGTCFRTSKPLVK"
     gene            complement(3228..>3561)
                     /locus_tag="PLU36_09265"
     CDS             complement(3228..>3561)
                     /locus_tag="PLU36_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HMZ46979.1"
                     /translation="SDNTERYSNMAVKKLVAHGHTVVAVGNKDTFIDTIPVYKEKIIE
                     DGIDTVTLYLSPKNQIPFYSYIISLKPKRIIFNPGTENEDLVTLAKQNNIQTIEACTL
                     VMLNTGQY"
BASE COUNT         1318 a          520 c          624 g         1099 t
ORIGIN      
        1 ccgcagtaat ttttgaagga acctgtagag gctgtgcctt tgaaagtaca accagtttta
       61 aaatatatga tagcctttct ttaataaaat tactatccat aaaaacaata gataataacc
      121 cacctgatat ggatggagga aacataagca aagaagtaga attagtacct ctacaaacag
      181 gcaatacaac aattaaagtg tacaaaatat ttggacaaga aactgaaaaa gaagatacaa
      241 tgcatgctac attatacaaa ataaaaataa ccaactaaac tttttttatg aataacttaa
      301 ttaaaaaata tttttatggc gattatggag aaatagaagc tggcagcaat gctagtaaca
      361 ataataaaaa tattgcatgg ccactatggg aggtgtttat tagaagtaaa caaggattag
      421 atcataaaca tgttggcagt ttacatgctg cagatgagca aatggctatt gaaaatgcaa
      481 gagatgttta tacaagaaga aatgaaggag taagtatttg ggtggttgaa agtaaatata
      541 ttactgcaag taacccagaa gaagcagggg agttgtacga accagcagtc aacaaaatat
      601 atcgtcatcc aacattttat aaattgccag atgaagtaga ttatatgtaa aaattatttt
      661 agtatcgtac aaatattaat ccattttctc cataaattat ttaaccatgc aagctgctaa
      721 agaaaatttt ttactctatc tagccgattc aacgcatata ctttctcata gaaatagtga
      781 atggtgtggt catgcaccta ttttagaaca agatattgca cttaccaata ttgcattaga
      841 tttattaggg cagtcaagaa atttttatca atatgccgca gaattaattg gtaataatgc
      901 aaccgaagat agccttgctt atttaaggat agaaagtgaa tacaagaatt tgttattggt
      961 agaattacct aaagaagatt gggcacatac agtattaagg caattttatt tttctgtttt
     1021 tctgttttat ttataccaac aactgcaaca aaataaagat gtacaaattg cagctattgc
     1081 cacaaaggca ataaaagaag ttgcttacca tgttcgttgg agtggggaat gggtaatacg
     1141 tttaggagat ggcacagaag aaagcaacca acgtatgcag tatgctgtgg aggcttacaa
     1201 aaactttaca ggagaatttt ttgtattaac caattatgaa aaacaaatgc aaatagattt
     1261 tgaattaata caacaacagt ggaacgataa agttgcagca gtttttaatg atgcaacact
     1321 ttcttttacc aatagtaatt ttcatcaggt aggaggtaaa gaaggaaggc atacagaata
     1381 cttaggatat attttaaccg atatgcaata tttgcaaaga acctatccca atgctacatg
     1441 gtaaatgaac aatccatatc agtagcaaat atttggaaga tagtagaaac tattcccgac
     1501 ccagaaatac ccgtaatttc aattgtagaa ctaggcgttg taagagaggt aaaaatagaa
     1561 aatgatatta ttgaaattat aattacacca acttatatag gctgccctgc aatggatatg
     1621 ataacgatgc atattaaact agcattaatg gaagcagggt ttaaaaatgt aaaagtaact
     1681 tctgttttat caccagcatg gaccacagat tggatgacgg aagaaacaaa aaacaaacta
     1741 aaagcctatg gtattgctcc acccaatact aaacaaatag tttgtacacc cgatttgttt
     1801 atacaagatg aggctgtagc ctgtccacat tgcaataact ttaatactaa aatggtatct
     1861 aggtttggca gtacagcctg taaagcttta tatttttgca acaattgtaa tgagcctttc
     1921 gattatttta aatgtcatta aaaataataa ttacatctac tttaccataa ataatatata
     1981 tgcccactgt tttatttgaa ataaaaaata atattggttt tataacctta aaccgcccag
     2041 agaaatttaa tgcttttaac agagaaatgg ctttgttatt acaagatatt ttaaccactt
     2101 gcaataacaa taaccaagta agagcagttt ttataaccgg tgcaggaaaa gcattttgct
     2161 ctggtcagga tttggcagaa gtagtggacc ctaatgggcc tggtatgaac agaattttag
     2221 cagaacatta taaccccatt attcaaaaaa taagaagcct ggaaaagcct gtaatagctg
     2281 cagtaaatgg tgttgcagca ggtgctggtg caaatattgc tttatgttgc gatattgtaa
     2341 tagctgcaga aaatgcaaca ttcattcagg cattttctaa aataggattg atacctgaca
     2401 gtggtggaac ttatttttta cctcgcctaa taggctggca aaaagctagt gcaataatgt
     2461 ttttaggaga taaaattaca gcagtggaag ccgacaaatg gggtatgatt tataaatttt
     2521 tttctgccga tgtttttgaa acagaagcct taaaaattgc aaacacttta gccgaaatgc
     2581 caaccaaagg tttgtatttt actaaacaag cattgagtaa ttcttttaca aatactttac
     2641 acgagcaatt ggttgatgaa gataaattgc aacaacaggc tgctgcaacc tttgatttta
     2701 aggaaggtgt tgcagctttt ttagaaaaaa gaaaaccaat ttttaaagga gaataatgaa
     2761 gtgtaagaaa gtatatactt tattattctt taaataattt aatacatgac caccacacca
     2821 caacaagttg ttgcacacat gatgcagcat gatttatttt ctcaatggtt aggaatagaa
     2881 gttttagaaa taaaagaagg atacagcaaa ataaaaatgt tggttagacc tgaaatggca
     2941 aatggcttta atattgtaca tggtggaatt gcctttagtt tggcagatag tgcatttgcc
     3001 tttgcatgta ataatagaaa caatctttct gtggcattag atacctctat taattttaca
     3061 aaaccagtac atgttggcga tatattaatt gcagaagcaa aagaattgca caatggaaaa
     3121 agtacaggtc tataccatat tacaattacc aatcaaaaca accatattgt agcaatgttt
     3181 aaaggcactt gttttagaac aagtaaacct ttggtgaaat aactttatca atattgccct
     3241 gtattaagca ttactaaagt acaagcttct atagtttgaa tattattttg tttggctagt
     3301 gtaactaaat cttcgttctc tgtaccaggg ttaaaaataa ttcttttagg ttttagagat
     3361 ataatgtaag aataaaaagg tatttggttt ttagggctta aataaagagt tactgtgtct
     3421 attccatctt caattatttt ttctttatat actggtatag tatcaataaa agtgtctttg
     3481 ttaccaacag caacaacagt atgtccatga gcaactaact tttttactgc catattgctg
     3541 tatctttctg tattatcaga a
//
LOCUS       DAOQPK010000223         3484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_19149_length_3484_cov_4.271216, whole genome shotgun sequence.
ACCESSION   DAOQPK010000223 DAOQPK010000000
VERSION     DAOQPK010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19149_length_3484_cov_4.271216"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..557
                     /locus_tag="PLU36_09270"
     CDS             207..557
                     /locus_tag="PLU36_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HMZ46980.1"
                     /translation="MSNKKSKADKDGFVYSTNPNFNFNNEEETVKTLLPNLQKLRIWL
                     DTKQRAGKTVTLITGFVGTLADLEKLAKQLKNFCGTGGNAKNNEIIIQGDQREKILQW
                     LIKNGYSNSKKAGN"
     gene            complement(586..1326)
                     /gene="rlmB"
                     /locus_tag="PLU36_09275"
     CDS             complement(586..1326)
                     /gene="rlmB"
                     /locus_tag="PLU36_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HMZ46981.1"
                     /translation="MFIPKKNIIVGRNPVVEALKQHLTIDKILVSKKASGEIVNEIKQ
                     LARANNVPVQYVPIEKINSLTNIQHQGIVAFKSAVRYFNLQHVIDFVVAKGETPLFLI
                     LDGVTDVRNIGAIARTAVCCGAQAIIIPDKGVGALNEDAVKTSAGALEQIHICRVNSL
                     MIAVDELRLNGISVFASEMTATTKLYNLNLNIPCAIVMGSEEKGVYPALMKICENTFH
                     IPMANNFESLNVSVATGMILYEAMKQRK"
     gene            complement(1326..1829)
                     /locus_tag="PLU36_09280"
     CDS             complement(1326..1829)
                     /locus_tag="PLU36_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46982.1"
                     /translation="MTKKIILAGIATVVMIVIMRLLSINLVTDYSPNGIVSFELAKSY
                     RDASAIMMAVGVKPLQINVAVDFAFIIAYCFFLYLCCKALLNQYTKQVLKTIGFIFLE
                     LSIFIGILDLVENIAMLITLGGYGTKISVSVSKWAAIAKFSIAGLVIVYILIASILLY
                     FSSRKKA"
     gene            complement(1853..2932)
                     /locus_tag="PLU36_09285"
     CDS             complement(1853..2932)
                     /locus_tag="PLU36_09285"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HMZ46983.1"
                     /translation="MSITQIPKFPVVKDSLHAELRKRVQQYFDDKGIKATGNISLFTK
                     AIILVTAFVLVYIHLIFFTPHWYIAIPECIILGCLIAAIGFNVMHDGSHGSFSKHKWK
                     NKLAAASLSMLGANHFMWNMKHNMLHHSFTNVDGVDDDIEIGVLMRMAPTQKRYKLHR
                     FQHFYFWFLYMLLYVFWIFFTDYHKYFTQKIGNVPLKKMATKDHIEFWLTKIYHGFIF
                     IALPIIMVGWFYWLMGFLVATLVSGFVLSIVFQLAHTVEHTHFPIADFDNNKLPDEFA
                     AHQIKTTANFATKNKIVSWLVGGLNFQIEHHLFPKISHVHYPAISKIVKSVCAEYQLT
                     YIEYPTVSRAIVAHVRFLKHLGKYD"
     gene            complement(3035..>3484)
                     /locus_tag="PLU36_09290"
     CDS             complement(3035..>3484)
                     /locus_tag="PLU36_09290"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HMZ46984.1"
                     /translation="GKNNQLLWHLPNDLKFFKNTTWGMPVVMGRKTFESLGNKPLNGR
                     LNIVITNQKKWNANGVVVVNSLDDAIFVAKSHSYKEMFIAGGGLIYNLVMNKADKIYL
                     TRVHTTIEGDTFFPEINEKQWQKVHDNFCNADEKHAFSYSFQLWERK"
BASE COUNT         1223 a          575 c          484 g         1202 t
ORIGIN      
        1 ctatcatagt atttttatta gctacacccg attttagaca atggttttgg ttagcactac
       61 ctggcacatg tacttacctt gcttattctt tagatttaat tgactaaata aatatcttta
      121 aacaattaac agcataatta catgttgttt aaatattttt aaagcattct taatattatt
      181 ttgaatgctt tttttatttt agctatatga gcaataaaaa atcaaaagca gataaagatg
      241 gatttgttta tagcactaat cctaacttta attttaacaa tgaggaggaa acagttaaaa
      301 cccttttacc taatttacaa aaattaagaa tttggttaga tactaaacaa cgagctggta
      361 aaacagttac attaataaca ggttttgtag gtacgttagc cgatttagaa aaattagcga
      421 aacaattaaa aaatttttgt ggtacaggtg gcaatgcaaa aaataatgaa attattatac
      481 aaggcgatca aagagaaaaa atactacagt ggcttattaa aaacggttat agcaatagta
      541 aaaaagcagg caattaaatt ataataacgt ttcaataaaa taatactact ttctttgttt
      601 cattgcttca tacaaaatca taccggtagc aacgctaaca tttaagcttt caaaattatt
      661 agccataggt atatgaaatg tgttttcaca aattttcata agggcaggat acacaccttt
      721 ttcttcgcta cccattacaa ttgcacaagg tatatttagg tttaaattgt acagttttgt
      781 tgtagcagtc atttcgctgg caaaaacact aataccgttc agccttagtt catccacagc
      841 aatcattaaa ctatttactc tgcaaatatg aatttgttca agagcaccag cacttgtttt
      901 aactgcatct tcatttaaag cacctacgcc tttatctgga attataattg cttgtgcacc
      961 acaacacact gcagtacgtg caattgctcc aatatttctt acatcggtaa cgccatctaa
     1021 aattaaaaac aagggggttt ctccttttgc tacaacaaaa tctattacat gttgtaggtt
     1081 aaaataacgc actgcacttt taaatgcaac aatgccttgg tgttgaatgt tagtaaggct
     1141 atttattttt tctattggaa catattgaac aggtacattg tttgctctgg caagttgttt
     1201 tatttcattc acaatttcgc cagatgcttt tttggaaact aatattttat ctatggttaa
     1261 atgctgcttt aaagcttcca ctacagggtt tcttccaaca ataatatttt ttttaggtat
     1321 aaacattagg ctttctttct agaagaaaaa tacaataaaa ttgaagcaat gagaatataa
     1381 acaataacca gcccagcaat agaaaattta gctatggcag cccatttgct aacacttaca
     1441 cttattttag tgccgtaacc tcctaaggta ataagcatgg ctatattttc tactaaatct
     1501 aatataccta taaaaatact tagctctaaa aatataaagc caatagtttt taaaacttgt
     1561 ttggtatatt ggttaagtaa agctttacaa cataaataaa gaaagaaaca ataagcaata
     1621 ataaaagcaa aatctacagc aacattaatt tgtaaaggct taaccccaac tgccatcata
     1681 atggctgatg catctctata acttttagct aattcaaaac ttacaattcc gttgggacta
     1741 taatctgtaa ctaaattgat tgatagcaaa cgcatgatta ctatcattac aactgttgca
     1801 atacctgcta aaataatttt ttttgtcatt tactgaaaag taaaaagtac aattagtcgt
     1861 atttacccaa gtgttttaaa aatcttacat gagctacaat tgccctactt actgttggat
     1921 attctatata agttaactga tattctgcac aaacagattt tacaatttta ctaattgcag
     1981 ggtaatgcac atgcgatatt ttaggaaaca aatgatgctc tatctgaaaa tttaaacctc
     2041 caactaacca acttactatt ttattttttg tagcaaagtt tgcagtagtt tttatttgat
     2101 gtgcagcaaa ctcatcgggt aatttgttgt tatcaaaatc tgcaatagga aagtgggtat
     2161 gctctacagt atgtgctaat tgaaatacaa tgctaagtac aaaaccagat accaatgttg
     2221 caacaagaaa acccattaac caataaaacc agccaaccat tatgatgggt aatgcaataa
     2281 atataaagcc atgataaatt ttagttagcc aaaactctat atgatcttta gtagccattt
     2341 tttttagagg aacattacct attttttgtg taaaatattt atgataatct gtaaaaaata
     2401 tccaaaaaac atacagcaac atatataaaa accaaaaata aaaatgttga aacctatgta
     2461 atttatatcg tttttgtgtg ggtgccatac gcattaaaac tcctatttca atatcatcat
     2521 ctactccatc tacattagtg aaactatggt gtagcatgtt gtgtttcata ttccacataa
     2581 aatgatttgc acctaacata cttaaagatg ctgctgccaa tttatttttc catttgtgtt
     2641 tactaaaact gccatggcta ccatcatgca taacattaaa accaattgct gcaattaaac
     2701 atcctaaaat aatacactct ggtatggcta tgtaccaatg tggtgtaaaa aatattaaat
     2761 gaatataaac tagtacaaat gcagtaacta aaataattgc tttggtaaat aagcttatgt
     2821 tgcctgttgc ctttattcct ttatcatcaa aatattgctg tacacgtttt cttaactccg
     2881 catgtaacga atcttttaca accggaaatt tgggaatttg agtgatggac ataggttttt
     2941 ttgaaaactt ttattaaagg cggcaagata acagtttatt gaataaaatt ggctgtacta
     3001 atgctaaagt tttaaactta gtggcaattt gaagttattt tctttcccaa agttgaaaac
     3061 tatagctaaa agcatgcttt tcatcggcat tacaaaagtt atcatgtact ttttgccact
     3121 gtttttcatt tatttccgga aaaaacgtat cgccttcaat ggtagtgtgt acacgggtta
     3181 aataaatttt atctgcttta ttcataacca aattatatat taatcctcca cctgctataa
     3241 acatttcttt atacgaatga ctttttgcta caaaaatggc atcgtctaaa ctatttacta
     3301 caacaacacc attggcattc catttttttt ggttggttat tacaatattt aatcgtccgt
     3361 ttaaaggttt attgccaagg ctttcaaatg tttttctacc cataacaact ggcatacccc
     3421 aagtagtatt tttaaaaaat tttaaatcat tgggtaagtg ccaaagtagt tggttatttt
     3481 tacc
//
LOCUS       DAOQPK010000224         3441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_19565_length_3441_cov_4.740992, whole genome shotgun sequence.
ACCESSION   DAOQPK010000224 DAOQPK010000000
VERSION     DAOQPK010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19565_length_3441_cov_4.740992"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PLU36_09295"
     CDS             complement(<1..424)
                     /locus_tag="PLU36_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HMZ46985.1"
                     /translation="MSTISKNTVAVILGGGAGTRLYPLTAARSKPAVPIAGKYRLVDI
                     PISNCINSNINRMFVLTQFNSASLNKHIKNTYHFSAFSTGFVDILAAEQTPDNQAWFQ
                     GTADAVRQSLRHIDTLDFEYVLILSGDQLYQMDFASMIN"
     gene            complement(567..1736)
                     /locus_tag="PLU36_09300"
     CDS             complement(567..1736)
                     /locus_tag="PLU36_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008621120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HMZ46986.1"
                     /translation="MSLYNQRGVSAQKEEVHNAIKQLDPGLYKNAFCKMYPDFLLGDE
                     NFVNISHADGAGTKSILAYLYWKETNDVSIWKGIAQDAVAMNLDDLLCVGVYDNFLFN
                     STIDRNKSKIPADVLEAIINGTQTFFNELKNYGINIHYLGGETADVGDVVRTIAVNGT
                     MTTRWPKNKLITNDKIDAGNVIVGFASYGKATYETAYNSGLGSNGLTSARHDVLSKYY
                     AEKYPETFEHSLSNNVVYIGKNKLTDTVQVENENIEIGKLLLSPTRTYAPLMKLLLKE
                     NFEDIYGVIHCSGGGQTKCLKYLPKPLLVIKNNLFDAPPIFKLIQENSNADFKEMYQV
                     FNMGHRLEIFTNETAAHKMIATANTLGIEAKIIGYTEQANKSKLILNGSFGEICF"
     gene            1838..2770
                     /gene="ribF"
                     /locus_tag="PLU36_09305"
     CDS             1838..2770
                     /gene="ribF"
                     /locus_tag="PLU36_09305"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HMZ46987.1"
                     /translation="MKVFKDLNQLLAFNNAVITIGTFDGVHEGHKKILKQLKEQAITV
                     NGESIVITFYPHPRKVVGDTTGVLLLTTLEEKIELLKKELIDNVVVVEFNEDFANQTA
                     EEYISDFLFKKFKPHTLIIGYDHHFGYKRKGNYELLEKFGQKLGFSVKEIDEKLLSET
                     AISSTRIRKALLQTDLYTAKQLLGYDYFIEGKVIEGDKLGRTIGYPTANLQLHNEEKL
                     VPGDGVYAVEIIWNKQPLQGMLYIGSRPVVNGKRRVIEVNIFNFNETIYQENLKIIFQ
                     SYIRADINMSNFEELKRQLDVDKTSALTFFKNSK"
     gene            complement(2778..>3441)
                     /locus_tag="PLU36_09310"
     CDS             complement(2778..>3441)
                     /locus_tag="PLU36_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46988.1"
                     /translation="IKVELNDEAKFLKLDDTAGITIQLRYPDNTVKRFAYGTDTLSFT
                     PATIGNNNIAIADFKPLLVQDGEYELMVSGKDKSGNKAGAKQYSVTFNVNNKPMISDV
                     FNYPNPFTSSTAFVFTLTGSQVPSNIRIQILTVTGKIVKEINKDELGPIRIGKNITEY
                     KWDGTDMYGQKLANGVYLYRVITNLNGNSLERFEFKDGFGDNINTQKYFKAGYGKMYL
                     MR"
BASE COUNT         1173 a          537 c          485 g         1246 t
ORIGIN      
        1 cgttaatcat agatgcaaaa tccatttggt acaattgatc gccactgaga attaacacat
       61 attcaaagtc taaagtatca atatgcctta agctttgtct tacagcatct gcagtgcctt
      121 gaaaccaagc ttgattatct ggcgtttgct ctgctgctaa aatatctaca aagcctgtac
      181 tgaatgcact aaaatgatat gtgtttttta tatgcttgtt tagtgatgca ctattaaact
      241 gcgtaagaac aaacattcta ttaatattgc tattaatgca attactaatt ggaatatcta
      301 ctaacctata ttttccggca attggtacag ctggtttgct acgtgctgct gttaatgggt
      361 ataaacgagt acctgcgcca ccaccaagta taactgctac tgtgttttta ctaattgttg
      421 acatacatca agtacattgg agttttaata tttaattatc caaatgtatg gtatgaagtt
      481 aactgttatc tattgatttt aaaaaataaa gcctacaatt tttaaaaata atttgaagtt
      541 ttaagatgct gcaattttat atgcttttaa aaacaaattt ctccaaagct tccatttaaa
      601 ataagtttgc ttttgttagc ttgctctgta tatccaataa tttttgcttc aatccctaat
      661 gtatttgcag ttgcaatcat tttatgtgca gcagtttcat ttgtaaaaat ttctagccta
      721 tgccccatat taaatacttg atacatttct ttgaaatctg cattagagtt ttcttgtatt
      781 aacttaaata ttggaggagc atcaaacaaa ttatttttaa taaccaataa tggtttgggt
      841 aaatatttca agcacttagt ttgcccacca ccactacaat gtattacacc ataaatatcc
      901 tcaaaatttt ctttcaataa taatttcatt aaaggtgcat aggtacgtgt tgggcttaat
      961 aaaagtttac caatttcaat attttcattt tcaacttgaa cggtatctgt taatttattt
     1021 ttaccaatgt acaccacatt attgcttaaa gaatgttcaa atgtttcagg gtatttttca
     1081 gcataatatt tacttaatac atcatgcctt gcactggtta agccattact tcctaatcca
     1141 ctgttataag cagtttcata agtagcttta ccataagatg caaagccaac aattacattt
     1201 cctgcatcta ttttatcgtt agtaattaat ttatttttag gccaacgagt agtcatggtg
     1261 ccatttactg caatagttct taccacatct cctacatcag ctgtttctcc tcctaaataa
     1321 tgaatattaa ttccgtagtt ttttagttca ttaaaaaatg tttgtgtacc attaataatt
     1381 gcttctaaaa catctgcagg tattttagat ttatttctat ctatggtaga attgaataaa
     1441 aaattatcat acacaccgac acaaagtaaa tcatctaaat tcattgcaac tgcatcttga
     1501 gcaatacctt tccaaatgct tacatcatta gtttctttcc aatataaata agccaaaata
     1561 ctttttgtac cagcaccatc ggcatgagaa atatttacaa aattttcatc gccaagtaaa
     1621 aaatctggat acattttgca aaaagcattt ttatatagcc ctggatctaa ttgttttatt
     1681 gcattatgca cttcttcttt ttgtgcagat actcctcttt gattatacaa tgacataatg
     1741 gtatttttaa gctctactaa aaacttgctt taaagtaatt aattgtttgc aaaagtaaat
     1801 ctatgtatcc tatttgccat tcactatttt tgtaaatatg aaggtattta aagatttaaa
     1861 ccaattactt gcatttaata atgctgttat taccattggc acttttgatg gagtgcatga
     1921 aggacataaa aaaatattaa aacagttaaa agaacaagcc attactgtga atggagaaag
     1981 tattgtaata actttttacc ctcatccaag aaaagttgtt ggcgatacaa caggtgtttt
     2041 attacttacc actttagaag aaaaaataga attacttaaa aaagaattga tagacaatgt
     2101 tgttgtagtg gagtttaatg aagattttgc taaccaaact gcagaggagt atatatcaga
     2161 ttttttattt aaaaaattta aaccacacac tttaattatt ggctacgatc atcactttgg
     2221 ttataaacga aaaggtaatt atgaattact tgaaaaattt ggacaaaaat taggttttag
     2281 tgtaaaagaa attgatgaaa aactgctttc agaaacagct ataagcagta ccagaattag
     2341 aaaagctttg ctacaaacag atttatatac tgctaaacaa ttgttgggat atgattattt
     2401 tattgaaggg aaagtaattg aaggagataa acttggcaga accataggct acccaactgc
     2461 taatttacaa ttacataatg aagaaaaact ggtacctgga gatggtgttt atgctgtaga
     2521 aattatttgg aataaacaac cattacaagg tatgttatat attggcagca ggcctgtggt
     2581 aaatggaaaa agaagggtaa ttgaagtaaa tatttttaat tttaatgaaa ctatttatca
     2641 agaaaattta aaaataatat tccaaagtta tatacgtgcc gatattaata tgagcaattt
     2701 tgaggagctt aaaagacaat tagatgtaga taaaacatct gcactaacat tttttaaaaa
     2761 tagtaagtaa aaaattctta cctcatcaaa tacattttac cataacctgc tttaaaatat
     2821 ttttgggtat ttatgttatc tccaaaacca tctttaaact caaatctttc taaactattg
     2881 ccatttaaat tggttattac tcgatatagg taaacgccat ttgctaactt ttgtccatac
     2941 atatcagttc catcccattt gtattctgta atattttttc ctattctaat gggtcctaat
     3001 tcatctttat taatttcttt tactatttta ccagttactg ttaaaatttg aattcgtatg
     3061 ttggagggta cttgactacc agttaatgta aacacaaatg ctgtagaaga agtaaaagga
     3121 ttgggataat taaacacatc agaaatcatt ggtttattat ttacattaaa tgtaacactg
     3181 tattgttttg cccctgcttt gttgccactt ttatctttac cacttaccat taattcatat
     3241 tctccatctt gtacaagtaa tggtttaaaa tcggcaattg ctatgttatt attgccaatg
     3301 gtagctggtg taaaacttaa agtatctgta ccataagcaa aacgctttac agtattatca
     3361 gggtaacgta attgaatagt tatgcctgca gtatcatcta attttaaaaa tttggcttca
     3421 tcattaagct ctactttaat t
//
LOCUS       DAOQPK010000225         3434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_19644_length_3434_cov_5.315774, whole genome shotgun sequence.
ACCESSION   DAOQPK010000225 DAOQPK010000000
VERSION     DAOQPK010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19644_length_3434_cov_5.315774"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PLU36_09315"
     CDS             <1..270
                     /locus_tag="PLU36_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HMZ46989.1"
                     /translation="VIFEEFKGTGNMELQLDRRLANKRIFPAIDLVSSSTRRDDLLLQ
                     PDVLQRMNILRLYINDMNTEEAMNELLKRMKGTKDNEEFLASMNK"
     gene            complement(344..2263)
                     /locus_tag="PLU36_09320"
     CDS             complement(344..2263)
                     /locus_tag="PLU36_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HMZ46990.1"
                     /translation="MYTKQRIKESGGVVTSPFSFQYLLMSDAIKHECGLAYIRLRKPF
                     SYYLQKYGTAMYGLNKLYLLMEKQHNRGQDGAGIAIVKLNTEAGYPYLHRIRSIAPQS
                     IAEIFATINNDLNEIEKYQPDIKQHPGLLKGHLPILGELMLGHLRYGTQGKNNVEFCH
                     PFIKRNIQPGRNLALAGNFNLVNTDELYDLLNIKPGIFQKQSDLAAMMETIHHFLIKE
                     DEQNPNNANIVALLQKAISLFDGGYTIGGLIGNGYSFILRDAHGIRPAYYFINDEFIV
                     AASERSAIRTVFNLNEKEVLELMPAHALIVDNAGNYTIHKIVEPKERRACSFERIYFS
                     RGSDEKIYRERIALGHQLSKTVLESIDYNLKQTIFSYIPNTAEVAFYGLVKGMEEYLN
                     KIKVDRILSWNGNFSQEKLEEMVNRKIRQEKIAIKDVKLRTFITEDKSRNEMVQHVYD
                     ITYGTVKDNEDTLVVIDDSIVRGTTLKQSIITMLARLRPKKIIIVSSAPQIRYPDCYG
                     IDMSKMGDFIAFKAAIELLKERGLEDIIINVQTKIVELQRNNQLHTENVVKQIYKPFT
                     PQEISNKIAALITPPALQIPIQVIFQDIENLHKSCPTNTGDWYFTGNYPTPGGNKVCN
                     KAFLNYLEGKDVRGY"
     gene            complement(2241..2792)
                     /locus_tag="PLU36_09325"
     CDS             complement(2241..2792)
                     /locus_tag="PLU36_09325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HMZ46991.1"
                     /translation="MNIEIISGSPRTESITKRIALFLDKEFKNKCDHNIGLIDLSNWN
                     LGLLQEVFKTVDTTPDKFKPLAERIFAADAFVLVTPEYNGSYSPALKNLFDHFPKQSR
                     KVFGIVTASTGALGGARSSQQMLLFVPALFGITSPYLLITPHVDKKFDENGNLLDLAF
                     QSAISTFKQEFLYLAESVHKTKN"
     gene            complement(2876..3400)
                     /locus_tag="PLU36_09330"
     CDS             complement(2876..3400)
                     /locus_tag="PLU36_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HMZ46992.1"
                     /translation="MLTYKIDAAHSEITFKVKHLMITNVTGNFTNFNASLTTTKEDFS
                     DAEITFEAATNSINTNNEQRDAHLKSDDFFNAEAYPTMHFKSTAFNKKAENEYILEGN
                     LTIRDITKPVVLNVEYNGTMVDPYGQTKAGFEITGKINRKDFNLKWSATTEAGGIVVS
                     DEVRLQLSAQMIKQ"
BASE COUNT         1062 a          549 c          509 g         1314 t
ORIGIN      
        1 gttatttttg aggagtttaa aggaacaggt aatatggaat tgcaattaga taggagattg
       61 gcaaataaaa gaatatttcc tgctattgat ttagtttctt cctctaccag aagagatgat
      121 ttattattgc aaccagatgt tttacaacgt atgaatattt tacgcctata cattaacgat
      181 atgaatacag aagaagcaat gaatgaattg cttaaacgta tgaaaggcac aaaagataat
      241 gaagagtttt tggcaagcat gaataaataa aaagggaatt aataataaaa taggctgcaa
      301 tagtaattgc agcctatttt tatgatataa ggttatataa taattaatat cctcttacat
      361 cttttccttc taaataattt aagaaagctt tgttgcatac tttgttacca cctggtgtag
      421 gataatttcc tgtaaaatac cagtcgccag tattggttgg gcaacttttg tgtaaatttt
      481 caatatcttg aaatattacc tgaataggaa tttgtaaagc aggtggtgta attaatgcag
      541 caattttatt cgaaatttcc tgcggtgtaa atggtttgta aatttgttta accacatttt
      601 ctgtatgtaa ttggttgttt cgttgtaact ctacaatttt tgtttgcaca tttataatta
      661 tatcttctaa acctctttcc tttaaaagtt caattgctgc tttaaaagca ataaagtcgc
      721 ccattttgct catgtcaatt ccataacaat caggatagcg tatttgtggg gcagacgata
      781 caataataat ttttttaggc cttaatcttg ccagcatggt aataatactt tgctttaaag
      841 tggtacctct tacaatgcta tcatctatta ctacaagtgt atcttcattg tcttttactg
      901 tgccataagt aatatcgtac acgtgttgta ccatttcatt acggctttta tcttcggtaa
      961 taaatgttct aagttttaca tctttaatgg caattttttc ttgcctaatt tttctgttca
     1021 ccatttcttc taatttttct tgcgaaaaat ttccattcca acttaaaatt ctatctactt
     1081 ttattttgtt taaatattct tccatgcctt taactaaacc ataaaaagca acttctgcag
     1141 tattgggtat ataagaaaaa atagtttgtt ttaaattata atcaatactt tctaaaactg
     1201 ttttactaag ttgatgccct aatgcaattc tttctctgta aattttttca tcacttcctc
     1261 tactaaaata aatacgttca aaactgcaag ctcttctttc tttaggctct acaattttat
     1321 ggatagtata attgcctgcg ttatccacta ttaaagcatg tgctggcatt agttctaaca
     1381 cttctttttc gttaaggtta aaaacagttc taatggcaga gcgttcactt gcagctacaa
     1441 taaattcatc attaataaaa taataagcag gccttatgcc atgagcatct cttaatataa
     1501 aactataccc attaccaatt aaaccaccta tagtatagcc accatcaaat aagctaatag
     1561 ctttttgtaa aagggctaca atgttggcat tattggggtt ttgttcatct tcttttatta
     1621 aaaagtgatg aatggtttcc atcattgcag ccaaatcgct ttgcttttga aatatgccag
     1681 gtttaatgtt taataaatca tataactcat cggtgtttac taagttaaaa ttgcctgcaa
     1741 gggctaagtt tctccctggt tgaatatttc ttttaataaa agggtggcaa aattctacat
     1801 tatttttccc ttgtgtacca taccttaaat gtcctagcat aagttctcct aaaatgggta
     1861 gatgtccttt tagtaagcca gggtgttgtt taatatctgg ttggtatttt tctatttcat
     1921 ttaagtcgtt atttattgta gcaaaaattt cagcaataga ttgtggagca atgcttctta
     1981 tacgatgtaa gtaaggataa ccagcttctg tattaagttt aactatagca attcctgctc
     2041 catcttgccc acgattatgt tgtttttcca tcaaaaggta caacttgttt aacccataca
     2101 ttgctgtacc atatttttga agataataag aaaaaggttt tcttaatctt atataagcca
     2161 aaccgcattc atgttttata gcatcactca taagtaaata ttgaaaagaa aatggcgaag
     2221 ttacaactcc gccactttct ttaattcttt gttttgtgta cactttctgc taagtataaa
     2281 aactcttgtt taaatgtact tatagctgat tgaaatgcaa ggtctaacag atttccattt
     2341 tcatcaaact ttttatcaac atggggcgtt attaataaat atggcgatgt tataccaaat
     2401 aaagctggta caaataataa catttgctga ctacttctgg caccaccaag ggcaccagtg
     2461 ctagcagtta caattccaaa aacttttctg ctttgtttag gaaaatggtc aaataagttt
     2521 tttaaagcag gtgagtaact gccattgtat tctggagtta ctaatacaaa tgcatcggca
     2581 gcaaaaattc tttctgccaa aggtttaaac ttgtctggtg tagtatctac tgttttaaac
     2641 acttcttgta ataagcctaa attccaatta cttaaatcaa ttaatccaat attatggtca
     2701 catttatttt taaattcttt atctaaaaat aatgctatcc ttttggttat actttctgtt
     2761 cttgggctac cactaatgat ttcaatattc attataccta gttttttaag aaaattttaa
     2821 acaaagatgc tgtttacgga taaaacactt ttattaatta agataaatat tttgattatt
     2881 gttttatcat ttgtgcactt aattgtaatc taacttcatc gctaactaca attccacctg
     2941 cttcggtagt agcactccat tttaaattaa aatcttttct gtttatttta ccggttattt
     3001 caaatcctgc ttttgtttgc ccgtatgggt caaccattgt gccattgtat tctacattta
     3061 atactactgg tttagtaata tctctaatgg ttaaattgcc ttctaatatg tactcgtttt
     3121 cagctttttt gttaaaggct gtagatttga aatgcatggt tggataggct tctgcattaa
     3181 aaaaatcatc actttttaaa tgtgcatccc tttgttcgtt attagtatta atactattgg
     3241 ttgctgcttc aaaagtaatt tctgcatcac taaagtcttc tttagtggtt gttaaagatg
     3301 cattgaagtt ggtaaaatta cctgtaacat tggtaatcat taagtgtttt actttaaaag
     3361 taatttcgct gtgtgcagca tcaattttgt aagttaacat agtttgtttt tttagtgtat
     3421 aaataatgta atta
//
LOCUS       DAOQPK010000226         3420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_19796_length_3420_cov_4.142942, whole genome shotgun sequence.
ACCESSION   DAOQPK010000226 DAOQPK010000000
VERSION     DAOQPK010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19796_length_3420_cov_4.142942"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PLU36_09335"
     CDS             complement(<1..200)
                     /locus_tag="PLU36_09335"
                     /inference="COORDINATES: protein motif:HMM:NF014015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46993.1"
                     /translation="MNNTKKAVFNWSGGKDSALALQKVLANNDYEVIALLTTITTGTN
                     KSSVHAIPLHLLQQQAKSIGIK"
     gene            complement(277..594)
                     /locus_tag="PLU36_09340"
     CDS             complement(277..594)
                     /locus_tag="PLU36_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46994.1"
                     /translation="MKIFIVDFSPNNNFLLHFQKEGLHYTIEKNDGGNAYKTVATELP
                     EKIFVNFASKPSHCIQTIKAILNRKTTSKIEIFFVDGDEKNNEKVKELGTSITYHQIK
                     NYI"
     gene            complement(591..1142)
                     /locus_tag="PLU36_09345"
     CDS             complement(591..1142)
                     /locus_tag="PLU36_09345"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HMZ46995.1"
                     /translation="MPKYISLLRGINVSGQKIIKMNELVALYNDVGFNNVTAYIQSGN
                     VVFSTTKTATQKLATRIEEKIKQHYSFEVSVLVLELLTFKSIVEHNPYSIATDKDSKQ
                     LYITFLYKPSVVTTIDALDKIKLPNEEVTIANNAVYFYFAQGYGKTKMNNNFIEQKLK
                     VIATTRNWKTCLQLLKIANTIQP"
     gene            complement(1235..3121)
                     /gene="rpsA"
                     /locus_tag="PLU36_09350"
     CDS             complement(1235..3121)
                     /gene="rpsA"
                     /locus_tag="PLU36_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HMZ46996.1"
                     /translation="MKFFIKQLNADAQETAGVEETTEVAATTNNLVVEETIAHDDFDW
                     SVDKRNVAVYSKEEKEKYDNVYEGTFVKIEDGEILNGDVVGLTKTDVVVNIGFKSDGL
                     ISLNEFRDVQGLKIGDSVEVMVVEKEDRNGNLHLSRKSARIFRAWERIMEVHKTGEVV
                     KGLVTSKTKGGLIVDVFGMETFLPGSQIDVKPVTDYDQFVGKTMEFKVVKINENIKNA
                     VVSHKALIESDIEAQRAEIIGKLEKGQVLEGTVKNITDFGAFMDLGGLDGLLYITDIS
                     WGRISHPSEVLKMDQKINVVVLDFDDDKKRISLGLKQLTPHPWDVLPEGLTEGNIVKG
                     KVVNIEDYGAFLEIQPGVEGLVHVSEITWANTPINAKEFFKLNDEHEAKVVTLDKENR
                     KMSLSIKQMTDDPWSTIENKYPESSKHKGLVKNITPYGVFVELETGIGGMIHISDLSW
                     LKRLNHPSDFTKVGEQIDIIVLSIDKENRKLQLGHKQLEEDPWAALQETFAIGSIHEG
                     LVTRRDDKGALVQLPYGLEGFAPNRHTFKEDGKQVQADETTQFMVIEFDGNEKRILVS
                     HTRIWEQLKAEEVEAEKKEAKVQAEQTKKAVKAVQSKVEKATLGDLGALADIKAKLDE
                     AKDK"
BASE COUNT         1054 a          601 c          444 g         1321 t
ORIGIN      
        1 aatttaatac ctatactttt tgcttgttgt tgtaataaat gcaatggtat agcatgtacc
       61 gaagatttat ttgtacctgt agtgatagtg gttaaaagtg ctataacttc ataatcgtta
      121 tttgctaaaa ctttttgtag tgctaaagca gaatcttttc caccactcca attaaaaaca
      181 gctttcttag tattattcat ctattaaaaa tatgctactc aagtaaaata aaatatttgt
      241 tgcttggtat ataatacaag cattcatttt acaactttaa atatagtttt tgatttgatg
      301 gtatgtaatt gatgtaccta gctccttaac tttttcgttg tttttttcat ctccatctac
      361 aaaaaatatt tcaatttttg atgtagtttt tctgtttaaa attgctttta tagtttgtat
      421 gcaatggctt ggtttgcttg caaaatttac aaatattttt tcagggagtt ctgtggcaac
      481 agttttgtac gcattgcctc catcattttt ttcaatagta taatgaaggc cttctttttg
      541 aaaatgaagt aagaaattat tatttggcga aaaatctaca ataaaaattt tcatggttga
      601 attgtattgg caatttttaa aagttgtaag caggttttcc aattacgagt tgtagctatt
      661 actttcaatt tttgttcaat aaaattattg ttcatttttg ttttaccata accttgtgca
      721 aaataaaagt acaccgcatt attagcaatc gtaacttctt cgtttggtag ttttatttta
      781 tctaatgcat caatagttgt aactacggaa ggtttgtaca aaaatgttat atacaattgc
      841 ttactatctt tatcagttgc aatagaatag gggttgtgct caacaatact tttaaaagtc
      901 aataactcta aaaccaatac agaaacttca aatgaataat gttgttttat tttctcttca
      961 attctggtgg ctagtttttg ggtagcagtt ttagtagttg aaaaaactac attaccactt
     1021 tgaatatagg cagttacatt attaaaacct acatcattgt atagtgcaac caattcattc
     1081 attttaataa tcttttggcc acttacattt attcctctca ataatgaaat atatttcggc
     1141 attctttttt taatgtaaag tattcattca aatatataaa aaaaacagcc ccaataatgc
     1201 aaggggctgt tgaagaaagt atatctaaaa ttatttactt gtcttttgct tcatcaagtt
     1261 tagctttaat atcagctaaa gcacctaaat ctcctaaagt agctttttca actttagatt
     1321 gaacagcttt tacggctttt ttagtttgtt cagcttgtac ttttgcttct ttcttttctg
     1381 cttctacctc ttctgctttt aattgttccc aaatacgagt atggcttact aaaattcttt
     1441 tttcattacc atcaaattca ataaccataa attgtgtagt ttcatcagcc tgcacttgtt
     1501 tgccatcttc tttaaaagta tgacggttag gagcaaatcc ttctaaacca taaggtaatt
     1561 gaactaaagc acctttatca tctcttctag taactaatcc ttcgtgaatg ctaccaattg
     1621 caaaagtttc ttgtaatgca gcccaaggat cttcttctaa ttgcttatgg cctaattgta
     1681 acttacgatt ttctttatcg atacttaaaa caataatatc tatttgttct ccaactttag
     1741 taaaatcgct agggtgattt aatcttttta accaacttaa atcgctaata tgaatcatac
     1801 caccaattcc agtttctaat tcaacaaaaa caccataagg agtaatgttt ttaactaaac
     1861 ctttatgttt actgctttct gggtatttat tttcaattgt gctccaaggg tcatcagtca
     1921 tttgtttaat agataaactc atttttctat tttctttatc taatgtaacc actttagctt
     1981 catgctcatc gttcagttta aagaactctt ttgcattaat tggagtatta gcccaagtaa
     2041 tttcgctaac gtgtactaaa ccttctacac ctggttgaat ttctaagaaa gcaccataat
     2101 cttcaatatt taccactttt ccttttacaa tatttccttc tgttaaacct tcaggtaaaa
     2161 catcccaagg gtgtggagtt aattgtttta aaccaaggct gatacgtttt ttatcatcat
     2221 caaagtctaa tacaaccaca tttattttct gatccatttt caatacttct gaaggatgtg
     2281 aaatacgacc ccaagaaata tctgttatgt ataataagcc atctaaacca cctaaatcca
     2341 taaaggcacc aaagtctgta atatttttta cagtgccttc taatacttgg cctttttcta
     2401 atttgccaat tatttctgca cgttgtgctt caatatcgct ttctattaat gctttgtgag
     2461 atactacggc atttttgatg ttttcattaa tttttaccac tttaaattcc attgttttgc
     2521 caacaaattg gtcataatcg gtaactggtt taacatcaat ttgagagcct ggtaagaagg
     2581 tttccatacc aaaaacatct acaattaatc cgccttttgt tttgctggtt actaagcctt
     2641 taacaacttc accagtttta tgaacttcca taatacgttc ccaagcacgg aagattcttg
     2701 cacttttacg gcttaaatgt aaattgccgt ttctgtcttc tttctctaca accattacct
     2761 caacagaatc gccaattttt aaaccttgta catcacgaaa ttcgtttaaa gaaattaatc
     2821 catcgctttt aaaaccaata tttacaacaa catcggtttt agttaagcca accacatcgc
     2881 catttaaaat ttcgccatct tcaatcttta cgaaagtacc ttcgtacaca ttatcgtact
     2941 tttctttttc ttctttgctg taaacagcaa catttctttt gtcaacactc caatcaaaat
     3001 cgtcgtgtgc aattgtttct tctactacaa gattgtttgt tgtcgcagca acttcagtag
     3061 tttcttcaac tcctgcagtt tcttgagcat ctgcgtttaa ttgttttata aaaaatttca
     3121 taataaaaat cccgttgttt ttttaaacgg gctgcaaagg taggtatttt atatataatt
     3181 aagctatttt agatatgttt ttatcataac ctatttttta tctaaaatac ctattataac
     3241 atttttttaa ttttagaatg atttaaaatt gttgtctaaa acttttatat gaaaaagaca
     3301 tgggtattat ttttagcgtt gtttttaaca actgcagttt tgcatagcca aacaaaacca
     3361 aagccagccg ataagaaggc ttctaaaaca gtacaaaaac aagcaaaaaa agaagtttca
//
LOCUS       DAOQPK010000227         3396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_20050_length_3396_cov_4.979647, whole genome shotgun sequence.
ACCESSION   DAOQPK010000227 DAOQPK010000000
VERSION     DAOQPK010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20050_length_3396_cov_4.979647"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="PLU36_09355"
     CDS             <1..785
                     /locus_tag="PLU36_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ46997.1"
                     /translation="SFSVFALNAQTVDEVINRFIEANGGLSKLTSINSLQIESSMNIE
                     QMGSTINVLTIKEKNKLFRIQSSSPMSSEESFTVITDTAGYTFVPAMNSPMGSMDASL
                     TKFKEDELEASSYQKDCEGLFAPLVNYAAKGHTATLDGTEKINGIECDKVNLKLKSGQ
                     EMTFYISKANAQVRRLQVSAPIALDMMGMSAMRRSFGGGGERSSRMANRKIDIDYEKY
                     KLFNGIPFPTKQSVSLGMMQLQLDNLSIKVNEPINARWYQVK"
     gene            complement(798..1925)
                     /locus_tag="PLU36_09360"
     CDS             complement(798..1925)
                     /locus_tag="PLU36_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HMZ46998.1"
                     /translation="MNRIYLDNAATTSLDTEVLNAMMPFLTTNFGNPSSIYSYGRESK
                     LGVENARKTVAKILNAHPSEIFFTSGGTESSNTAITASVRDLGCKHIISSPIEHHATL
                     HTIEYMHKTGEATLSYVKLLPNGHIDLENLEFLLANSKEKTLVTLMHANNEIGNIINI
                     NSVGELCKKYNAIFHSDTVQTVGHFPFDLKNTFVHFITGAGHKFHGPKGVGILYINQN
                     IKIEPFVHGGSQERNMRAGTENLYGIVGFAKALELATENYEKDSQYINSLKLFMKELL
                     QKNIEGVSFNGDVDGKSLYTVLSVNFPKTEKSDMILFNLDINNICASGGSACSSGADA
                     GSHVVGALHNNSHTVTVRFSFCKHNTKEEIETVVEKIKTLI"
     gene            2077..2718
                     /gene="pdxH"
                     /locus_tag="PLU36_09365"
     CDS             2077..2718
                     /gene="pdxH"
                     /locus_tag="PLU36_09365"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HMZ46999.1"
                     /translation="MNPHIANIRTNYLMASFSEEDASYNPFEQFEKWWNQAIKSEIGE
                     INAMTLATVSAQGTPSARIVLLKDFDKNGFVFYTNYLSDKGKEIAQNPKVALVFFWKE
                     LERQVRIEGIAEKVSNEESDAYFNSRPISSRIGAWASHQSAVIPYRQVIEQNEKKYAE
                     IFGTENIPRPDHWGGYIVKPTLIEFWQGRASRLHDRLQYTFESNVWVRERLSP"
     gene            complement(2715..3227)
                     /locus_tag="PLU36_09370"
     CDS             complement(2715..3227)
                     /locus_tag="PLU36_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HMZ47000.1"
                     /translation="MSEVFTNKVAESGILTVDLEDFLPKEPIENFDLKEYLFMGLILK
                     EKDFRAALLQINWSYYSNKIVGIYCSTDAIIPVWAYMLVASYLQPYAIYVATCDKEQL
                     KENLLLQKIETLPIQEYTDKRVVIKGCGEVAIPASAYTAITFKLRPIVKSIMYGEPCS
                     TVPVYKKKLN"
BASE COUNT         1178 a          501 c          536 g         1181 t
ORIGIN      
        1 tatcattttc agtatttgct ttaaatgcac aaacagttga tgaggttata aatagattta
       61 ttgaagccaa tggagggtta agtaagttaa catctattaa tagtttgcag atagaaagtt
      121 ctatgaatat tgaacaaatg ggttctacta ttaatgtact taccattaaa gaaaagaata
      181 aattatttcg tattcaaagc agtagcccaa tgagcagcga agaaagtttt actgtaatta
      241 ccgatactgc tggttataca tttgttcctg caatgaactc tccaatgggt agtatggatg
      301 cctctcttac aaaatttaaa gaagatgaat tagaggcttc ctcttatcaa aaagattgtg
      361 aaggcttgtt tgcaccattg gtaaattatg cagccaaagg gcatacagct actttagatg
      421 gaacagaaaa aattaatgga atagaatgtg ataaagtaaa cttaaaacta aaatctggtc
      481 aagaaatgac tttttatatt tctaaagcaa atgcacaagt aagaaggttg caggttagtg
      541 caccaattgc tttagatatg atgggtatga gtgcaatgag aagatcattt ggtggtggtg
      601 gagaaagaag ctctagaatg gctaacagaa aaattgatat tgattatgaa aaatataagc
      661 tatttaatgg cattcctttt cctacaaaac aatcagtttc attaggaatg atgcaactac
      721 aactagataa tttaagtatt aaagtaaacg aaccaataaa tgcaagatgg tatcaggtta
      781 aatagctgaa ccaaagttta aataagagtt tttattttct caaccacagt ttcaatttct
      841 tcttttgtat tgtgtttaca aaaactaaat ctaactgtaa cagtatgact gttgttgtgt
      901 aatgcaccta caacatggct tcctgcatct gcaccactag agcatgcact gccaccactt
      961 gcacaaatat tattaatatc taaattaaaa agtatcatat cgcttttttc tgttttagga
     1021 aagttaacgc ttaacacagt atataaactt tttccatcaa catctccatt aaagcttact
     1081 ccttcaatat ttttttgtaa caactctttc ataaaaagct ttaagctatt tatatattgg
     1141 ctgtcttttt cataattttc tgttgctaat tctaaggctt ttgcaaagcc aacaataccg
     1201 tataaatttt ctgttccggc tctcatattc ctctcttggc tgccaccatg cacaaatggt
     1261 tctattttaa tattttgatt gatgtataaa atgccaacac cttttgggcc atgaaattta
     1321 tgcccagcac cagtaataaa atgaacaaaa gtatttttta aatcgaaagg aaaatggcca
     1381 acagtttgaa cagtatcgct atgaaaaatg gcattgtatt ttttgcaaag ttccccaaca
     1441 gaattgatgt taattatatt tccaatttcg ttgttggcat gcattagggt aactaatgtt
     1501 ttttcttttg aattggctaa caaaaactct aaattttcta aatcaatatg cccatttggt
     1561 agcagtttta catagcttaa agtagcctca ccagttttat gcatatattc aatagtatgt
     1621 aaagtagcat gatgttcaat aggagatgaa ataatatgtt tgcatcctaa atctcttaca
     1681 cttgcagtaa ttgcagtatt cgaactttcg gtaccaccac ttgtaaaaaa tatttctgat
     1741 ggatgtgcat ttaaaatttt agctactgtt tttcttgcat tttcaactcc aagtttactt
     1801 tctctgccat agctataaat agaagatgga ttaccaaagt tagttgtaag aaaaggcatc
     1861 atagcattta acacctctgt gtctaaactt gttgttgctg cattgtctaa ataaattcta
     1921 ttcattgaag catttattat taaaagattt agggcaaaag tataaaattt atttttttag
     1981 cattatagtt ttttgcattt cataaaccat aaaaaagtaa aaatgttttc tgtatgtttg
     2041 tattttaaag cacattacta tcttttatta ttgtttatga accctcacat tgcaaatatc
     2101 agaactaact atttaatggc ttctttctct gaagaagatg ccagttataa cccttttgaa
     2161 cagtttgaaa aatggtggaa tcaagcaatt aaaagtgaaa ttggtgaaat aaatgcaatg
     2221 acattggcta cagtttctgc acaaggtaca ccaagtgcaa gaattgtact tttaaaagat
     2281 tttgataaga atggatttgt gttttatacc aactatttaa gcgataaagg aaaagaaatt
     2341 gctcaaaatc caaaagttgc tttagtattt ttttggaagg agttagaacg tcaggtacga
     2401 attgaaggaa ttgctgaaaa agtaagtaat gaagaaagtg atgcatattt taatagcaga
     2461 ccaataagta gtagaattgg tgcttgggca tcgcatcaaa gtgctgttat accttatagg
     2521 caagttattg aacaaaacga aaaaaaatat gctgaaattt ttggaacaga gaatatacca
     2581 agaccagatc attggggagg atatatagta aaacctacat taatagaatt ttggcaggga
     2641 agagcaagca ggttgcacga tagactacaa tacacttttg aaagtaatgt ttgggtaagg
     2701 gaaagattat ctccttaatt tagtttcttt ttgtaaactg gaactgtgct acatggttcg
     2761 ccatacataa tactttttac aataggtctt aatttaaatg taattgctgt ataggcagat
     2821 gcaggtattg ctacttcgcc acaacctttt attacaactc ttttatctgt atattcttgt
     2881 attggtaatg tttctatttt ttgaagaagt agattttctt ttaattgttc tttatcacag
     2941 gttgcaacat aaattgcata aggttgcaaa taagaagcaa ctaacatata tgcccaaaca
     3001 ggtataatag catctgtgct acaatatatg ccaacaattt tgttgctata ataactccaa
     3061 ttaatttgta gtaaagcagc tctaaaatct ttttctttta aaattaaacc cataaaaaga
     3121 tactctttta aatcaaaatt ttctatcggt tcttttggta aaaaatcttc taaatcaaca
     3181 gttaaaatac cgctttcggc aactttattt gtaaatactt cactcatatt gcaaagttat
     3241 ttttttatta taaaacaatg tttcgtaata agtaaaagaa ataaatatat agtttttatt
     3301 cttttaaaat atctctaata aaattatgat actcatcaaa ctttggtaaa ttattatgcc
     3361 caccaccatt tatttttatt aatgtaactt ttgatg
//
LOCUS       DAOQPK010000228         3393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_20084_length_3393_cov_4.976034, whole genome shotgun sequence.
ACCESSION   DAOQPK010000228 DAOQPK010000000
VERSION     DAOQPK010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20084_length_3393_cov_4.976034"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..>3393
                     /locus_tag="PLU36_09375"
     CDS             247..>3393
                     /locus_tag="PLU36_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HMZ47001.1"
                     /translation="MDSVMQNKVRIVTAASLFDGHDAAINIMRRIMQSKGAEIIHLGH
                     NRSVKEIVECAIEEDAHGIAITSYQGGHVEFFKYMKDLLEENSCGHIKIFGGGGGTIL
                     PDEVRELHDYGITKIYTPDDGRHMGLEGMIEDVINTLKLTSLITHNWNGKLPLDEVKD
                     VRKVARAITLAENGNDYKDLVKQLATANKHQAAVLGITGTGGAGKSSVTDEIVRRFLH
                     TFTDKTIAVISVDPSKKKTGGALLGDRIRMNAISHPRAYMRSLATRDDNTALSAYVKD
                     AIEICKKANYDFIILESAGVGQSDASILDYCDISMYVMTPEYGAASQLEKINMLDYAD
                     VVCINKFDKAGALDAYQDVCKQYKRNHKLFTAKNEDVPVIGTIASKFNDEGVNHLFDK
                     LLQVIETKTNIRFGIFTHQATAKVTDAVIPAKRVRYLSEISSSIRDYNQLAIDQCLLA
                     TKLYKLQGSLEILKTTTDTALLEQLQQQINFYTEQLTPVAKKLIHNWEEKKTAYSKDF
                     YEYKVRDKVIQQEMFTTSLSGTRIPKVVLPKYKDWGDLLKWQCQENFPGSFPFTSGVF
                     PLKREGEDPTRMFAGEGGPERTNKRFHYVSIGQPAHRLSTAFDSVTLYGEDPTHRPDI
                     YGKIGNSGVSIATVDDAKKLYSGFDLCHPKTSVSMTINGPAPIILAFFMNAAIDQECE
                     KYIEQNNLWATVEKISKEKYITSNAPLYYNPSSPERLPEGNNGLGLKLLGLSGEEVLP
                     KEIYEQLKAKALQSVRGTVQADILKEDQAQNTCIFSTEFALKLMGDVQEYFIQQNVRN
                     FYSVSISGYHIAEAGANPITQLAFTLANGFTYVEYYLSRGMNIDDFAPNLSFFFSNGM
                     DAEYSVIGRVARRIWAKAMKYKYKANKRSQMLKYHIQTSGRSLHAQEIDFNDIRTTLQ
                     ALYAIYDNCNSLHTNAYDEAITTPTEESVRRAMAIQLIINKELGSAKTENFIQGSFAI
                     EELTDLVEEAVLTVFDKITERGGVLGAMERMYQRNKIQEESMHYEMQKHTGELPLIGV
                     NTFLNKKGSPTITPTEVIR"
BASE COUNT         1269 a          513 c          640 g          971 t
ORIGIN      
        1 ggatctactg aatagcaaca acaaagaaca gatagattcc tcgtaaactc ggaatgacag
       61 agaattaaag attcctcgca agctctgaat gacaatactt tatatacaaa caatatgatt
      121 tggcttttat aactttgaac tgctcttaat tataacccaa tgtttattta aacatagttt
      181 gtaaaactaa caagcgttat cctttactcg taaaagccta attttgccgt ttttaatatg
      241 taatctatgg atagtgtaat gcaaaacaaa gttcgtattg taactgctgc aagtttattt
      301 gatggacatg atgctgcaat aaatattatg cgtagaataa tgcaaagcaa aggggcagaa
      361 attattcatt tagggcataa cagaagtgta aaagaaattg tagaatgtgc tattgaggaa
      421 gatgctcatg gtattgctat cacatcgtac caaggtgggc atgtagagtt ttttaaatac
      481 atgaaagatt tgttagagga aaatagttgt gggcatatca aaatttttgg tggtggtggc
      541 ggaaccattt taccagatga agtaagagaa ttgcatgatt atggaattac aaaaatttat
      601 acccccgacg atggcagaca catgggcctt gaaggaatga ttgaagacgt aattaacacc
      661 ttaaaactaa ccagcttaat tacacataat tggaatggaa agctaccttt agacgaagtg
      721 aaagatgtaa gaaaagttgc tcgagctatt acacttgcag agaacggtaa tgattataaa
      781 gatttggtaa aacaacttgc tacagcaaat aaacatcaag cagcagtttt aggaattaca
      841 ggtacaggtg gtgcaggaaa aagctcagta accgatgaaa tagttagacg atttttacac
      901 acttttaccg ataaaaccat tgctgttatt tcggttgacc ccagcaagaa aaaaacaggt
      961 ggtgcattat taggcgacag aattagaatg aatgcaatat cacatccaag agcttatatg
     1021 cgtagccttg ctaccagaga tgacaatact gctttaagtg cttatgtaaa agatgctata
     1081 gaaatttgca aaaaggccaa ctacgatttt attattttag aaagtgcagg tgtaggacaa
     1141 agcgatgcaa gtattttaga ttattgtgat ataagcatgt atgtaatgac cccagaatat
     1201 ggtgcagcat cgcaactaga aaaaataaac atgctagatt atgccgatgt agtttgtatt
     1261 aataaatttg acaaagctgg tgctttagat gcatatcaag atgtatgtaa acaatacaaa
     1321 agaaaccata agctttttac tgcaaaaaat gaagatgtgc ctgttatagg caccatagca
     1381 agtaagttta acgacgaggg tgtaaaccat ctttttgata aattattaca agtaatagaa
     1441 accaaaacca acataaggtt tggcatattc acccatcaag caacagcaaa agttaccgat
     1501 gctgtaatac cagcaaaaag agtgagatat ttaagtgaaa tatcctcctc tattagagac
     1561 tataaccaat tagctataga ccaatgcttg cttgcaacta aattatataa actacagggc
     1621 agtttagaaa ttttaaaaac caccaccgat actgctttgc tagaacagtt acaacaacaa
     1681 ataaattttt ataccgaaca gcttacacct gtagcaaaaa aattaattca taattgggag
     1741 gaaaagaaaa cagcttatag taaagatttt tatgaatata aagtaagaga taaagttatt
     1801 cagcaagaaa tgtttacaac tagcttatcc ggcacaagaa tacctaaagt tgttttacca
     1861 aaatataaag attggggaga cttattaaaa tggcaatgtc aggaaaattt tcctggaagt
     1921 tttccattta catcgggtgt atttccttta aaaagagaag gtgaagaccc tactagaatg
     1981 tttgcaggag aaggcggacc tgaaagaacc aataaacgtt ttcattatgt aagtattggg
     2041 cagccagcac atcgtttaag tactgctttt gatagtgtta ctttatacgg agaagaccca
     2101 actcatcgcc cagatattta tggaaaaatt ggtaacagtg gcgtaagcat tgctactgta
     2161 gatgatgcta aaaaattata cagcggtttt gatttatgtc atcctaaaac ttctgtatca
     2221 atgaccatta atggtccagc tcctatcatt cttgcatttt tcatgaatgc tgcaattgat
     2281 caggaatgtg agaaatatat agaacaaaat aatttatggg caaccgttga aaaaattagt
     2341 aaagaaaaat acattacctc aaatgcacct ctttattaca acccatcatc gccagaacgt
     2401 ttgccagaag gcaataatgg tttaggttta aaattattag gtttaagcgg agaagaggta
     2461 ttgcctaaag aaatatatga acaacttaaa gcaaaagctt tacaaagtgt tcgtggtaca
     2521 gtacaggcag atattttaaa agaagaccag gctcaaaata cttgtatttt ttctacagag
     2581 tttgcattaa aattaatggg agatgtacaa gaatatttta tacaacaaaa tgtaagaaat
     2641 ttttatagtg tttctatcag tggttaccat attgccgaag ctggtgcaaa ccctattaca
     2701 caattagctt ttacattggc aaatggtttt acctatgtag aatattattt aagcagagga
     2761 atgaatatag acgattttgc acctaacctt tctttcttct tcagcaatgg tatggatgca
     2821 gaatacagtg taattggcag ggttgcaaga cgtatttggg ctaaagcaat gaagtacaaa
     2881 tacaaagcaa ataaacgcag ccaaatgttg aaatatcata ttcaaacaag tggtagaagt
     2941 ttgcatgctc aagaaataga ttttaacgat ataagaacca ccttgcaagc attgtatgct
     3001 atttatgata attgcaattc attacacact aatgcttatg atgaggcaat tactacacca
     3061 acagaagaaa gtgtaagaag agcaatggct atacagttaa ttattaataa agaattaggc
     3121 agtgctaaaa cagaaaattt tattcagggt agttttgcaa tagaagaatt aaccgatttg
     3181 gtggaagaag ctgtacttac tgtatttgat aaaattacag aacgaggtgg cgttttagga
     3241 gcaatggaaa gaatgtatca aagaaataaa atacaagaag aaagtatgca ctacgaaatg
     3301 caaaaacata caggagaact tccattaatt ggggtaaata ccttcttaaa taaaaaaggt
     3361 agcccaacca ttactcctac agaagtaatt cgt
//
LOCUS       DAOQPK010000229         3385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_20166_length_3385_cov_3.926126, whole genome shotgun sequence.
ACCESSION   DAOQPK010000229 DAOQPK010000000
VERSION     DAOQPK010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20166_length_3385_cov_3.926126"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PLU36_09380"
     CDS             complement(<1..276)
                     /locus_tag="PLU36_09380"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HMZ47002.1"
                     /translation="MNKVVANAQAAIADIPSGSTIMLGGFGLNGIPENCIAALVEKGI
                     NNLTCISNNAGVDDFGLGLLLKTKQIKKMISSYVGENAEFERQLLQGE"
     gene            455..1153
                     /locus_tag="PLU36_09385"
     CDS             455..1153
                     /locus_tag="PLU36_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HMZ47003.1"
                     /translation="MFIAIMFIAFPFILISSSFGIKGGNVIYKICNVWARVWYFFVGV
                     KHQEIYETEHEVNKQYIFVANHISYMDIPPIVTSIHQPFRALGKYEMVKVPIFGWIYR
                     SAVILVDRSSAEKRSKSVRALKAAIQHGISVFIFPEGTFNMTEHPLKNFYDGAFRIAI
                     ETQTPIKPMLFVDSLKRLHYKSLFELTPGINRTIYLEEVSVDGLTMKNLHELKEVVYK
                     KMEEGLKRYNNIKV"
     gene            1159..>3385
                     /gene="priA"
                     /locus_tag="PLU36_09390"
     CDS             1159..>3385
                     /gene="priA"
                     /locus_tag="PLU36_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HMZ47004.1"
                     /translation="MNNDFQNINNDNSETKKLFASVIIPLSLPETYTWEIPDQFINSV
                     KVGVRVEVQLRNKKYAGIIKSITNIVPTSFKPKHILNILDEEPLLYEKQLLLWRWIAN
                     YYMCTEGDVMQAALPANLKLTGESVLVWNEENGIDLNNYTFLSDEEYLITEALELKKE
                     LKISEVQKIIEASHVYPIIRNLIDKKVCFIWEELKEKYKPKTDTFILLHPVFQQESAL
                     EKLLNEWSKAPKQLELLLGYLHFIKTEGEVTQSKLLQKTNASAAQLKALVDKKILITE
                     KRNVNRINTLPKQVVIDFTLSDAQQKAFSNIQQQLQHHNVCLLHGVTASGKTQIYIKL
                     IEQYLQQDKQVLFMLPEIALTTQIIRRIQKHFGGYIAIYHSKFNSNERVELWNRVKTN
                     DIRIVLGARSALFLPFNNLGLIIVDEEHDSSYKQQDPAPRYHARDAAIYYASLFKNET
                     ENNTAKVVLGSATPSIETYFNAQNGKYGLVQLNERFANVDMPTIQLIDLKKVIANEQI
                     NQNSKNEQLKIALSPELLNAMAISLKNKKQIILFQNRRGYSPYMICNACGWIPQCKHC
                     DVTLTFHKAKNKLSCHYCGTSYPVLNSCVVCGSHHFTNKNFGTEKIEEILAEHFTDAK
                     IARMDIDSIKGKNAHDNLIKLFEQQRIDILVGTQMVVKGLDFEHVNLIGIMDADGLLS
                     FADFRVNERAFQLMEQVSGRAGRKDGVGTVYIQANNIHHPVIEFVQQHNYDRLYKFEL
                     EG"
BASE COUNT         1260 a          450 c          548 g         1127 t
ORIGIN      
        1 ctctccttgt aatagctgtc tttcaaattc tgcattttct ccaacatagc tactaatcat
       61 ttttttaatt tgtttggttt ttagtaataa tcctaaacca aaatcatcaa caccggcatt
      121 attagaaata caagttaaat tatttattcc tttttcaact aatgctgcta tgcaattttc
      181 tggaatgccg tttaagccaa atcctcctaa cattattgta ctgccacttg gtatatctgc
      241 aatggcagct tgtgcatttg ctactacttt attcatacta aattcatttg aataacaaat
      301 ataagtctat tggcatttac agtataaaca atgcgttttt gaaagatgaa aatttgaata
      361 aattattctt tattactttc tattatcttt atttgctaat atgaaagggt taataaaact
      421 aattcaattt atatattgtg tatatgcttt gctaatgttt attgcaatta tgtttattgc
      481 atttccattt attcttattt cctcttcatt tggaattaaa gggggtaatg taatttataa
      541 aatttgtaat gtatgggcaa gggtatggta tttttttgta ggtgtaaaac atcaagagat
      601 ttatgaaacg gagcatgaag taaataagca atatattttt gttgctaacc atatttctta
      661 tatggatatt ccacccattg taacttctat tcatcagcca tttagggctt tagggaaata
      721 tgaaatggtt aaagtaccca tatttggttg gatttacaga tctgctgtta ttttggtgga
      781 tagaagtagt gcagagaaaa gatctaaaag tgtaagagca ttaaaggcag ctattcaaca
      841 tggtatttca gtttttattt ttcctgaagg aacttttaat atgacagagc atcctctaaa
      901 aaatttttat gatggtgctt ttagaattgc aattgaaacc caaactccca taaagcctat
      961 gttatttgta gattcgctaa aaagattaca ttataaaagc ttatttgaat taacacctgg
     1021 tattaacaga acaatttatt tagaagaagt ttcagtagat gggttaacta tgaaaaattt
     1081 gcatgagcta aaagaggtag tgtataaaaa aatggaagaa ggattaaaga gatataacaa
     1141 tattaaagtc taaaaaattt gaacaacgat tttcaaaata ttaataatga caattcggaa
     1201 acaaaaaaac tatttgcttc tgttattatt cctttatcct taccagaaac atatacctgg
     1261 gaaatacctg atcaatttat taattctgta aaagtaggcg ttagggtaga ggtacaatta
     1321 agaaataaaa aatatgctgg cattataaaa tctattacca acattgttcc tacaagtttt
     1381 aaaccaaagc atattttaaa tatattagac gaagagccat tgttgtacga aaagcaatta
     1441 ttgctttggc gttggattgc caattattat atgtgtacag aaggagacgt tatgcaagct
     1501 gcattacctg ctaatttaaa gttaacagga gaaagtgttt tagtttggaa tgaggagaat
     1561 ggtattgatt taaataacta caccttttta agtgatgaag aatatttgat aacagaagcc
     1621 ttagagctaa aaaaagaact taaaataagt gaagtacaaa aaattattga agcaagtcat
     1681 gtttatccaa ttatcagaaa cttgatagat aaaaaagtat gttttatttg ggaagagttg
     1741 aaagaaaaat ataagcctaa aacagataca tttattttgt tacaccctgt ttttcaacaa
     1801 gagagtgcat tagaaaaatt attgaatgaa tggagtaaag cacccaaaca attagaactt
     1861 ttattaggct atttgcattt tataaaaaca gaaggagaag ttacacaatc taaactctta
     1921 caaaaaacaa atgcatctgc tgctcaatta aaagcattag tagataaaaa aattttaata
     1981 acagaaaaga gaaatgttaa tagaataaat actttaccga agcaagtggt tattgatttt
     2041 actttatctg atgctcagca aaaagcattt tccaatattc agcaacaact acaacatcat
     2101 aatgtttgct tattacatgg tgttactgca agtggtaaaa cacaaattta tataaaacta
     2161 attgagcaat atttacaaca agataaacag gttttgttta tgctgccaga aattgctttg
     2221 accacccaaa ttattagaag aattcaaaaa cactttggtg gttacattgc aatttatcat
     2281 tctaagttta actctaatga aagggttgaa ctttggaata gagtaaaaac aaacgatatt
     2341 agaattgtac ttggagcaag aagtgctctg tttttaccat ttaataattt aggattaata
     2401 attgttgatg aagaacatga ttctagctat aaacaacaag acccagcacc tcgttatcat
     2461 gcaagagatg ctgcaatata ttatgcttct ttatttaaaa acgaaacaga aaataataca
     2521 gctaaagttg tattaggtag tgcaacacca tccatagaaa cttattttaa tgcacaaaat
     2581 ggaaaatatg gtttggtaca attaaatgaa cgatttgcaa atgtagatat gccaaccatt
     2641 caattaattg acttaaaaaa ggtaattgcc aacgaacaaa tcaatcaaaa ctctaaaaat
     2701 gaacaactga aaattgcttt atcgccagag ttattaaatg caatggctat ttcattaaaa
     2761 aataaaaagc agataattct ttttcaaaat agaagaggct acagcccgta tatgatttgc
     2821 aatgcttgtg gttggattcc tcaatgcaag cattgcgatg ttacattaac ttttcataaa
     2881 gctaaaaaca aattaagttg ccattattgt ggtacttctt accctgtatt aaactcctgt
     2941 gtggtgtgtg gtagtcatca ttttaccaat aaaaattttg gtaccgaaaa aattgaagaa
     3001 attttagcag aacattttac cgatgcaaaa attgccagaa tggatataga cagcattaaa
     3061 ggcaaaaatg ctcatgataa cttaattaaa ctttttgaac aacagcgaat agatatttta
     3121 gtaggaacac aaatggtggt aaaagggttg gattttgaac atgttaattt aattggaatt
     3181 atggatgctg atggattgtt aagctttgca gattttagag taaacgaaag agcctttcaa
     3241 ttaatggagc aagttagtgg ccgtgcaggt agaaaagatg gagttggaac tgtttatatt
     3301 caagcaaata atattcatca tcctgtaatt gaatttgttc aacaacataa ttacgataga
     3361 ttatataaat ttgaattaga aggaa
//
LOCUS       DAOQPK010000230         3359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_20427_length_3359_cov_5.376816, whole genome shotgun sequence.
ACCESSION   DAOQPK010000230 DAOQPK010000000
VERSION     DAOQPK010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20427_length_3359_cov_5.376816"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1699
                     /locus_tag="PLU36_09395"
     CDS             <1..1699
                     /locus_tag="PLU36_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ47005.1"
                     /translation="ITENTPEYRGVAGNMGFNFIPGPWIENIQVTKGIGSVVNGYESI
                     AGQINVELKKPNQKQPFFINGYVNTMGRMEGNFVTSKQLNDKWSTATLVHANGVIAKN
                     DMNKDGYLDIPKGHQINIANRWDYSNKNGLGIHWILKGVSDRRIGGNKNFTTADKLTT
                     NNYGVMLNNDALNATAKIGYIFPKYKHRSIGLILSGSYSNINNVYGLTNYKAKQTGFY
                     SNLIYQSIINNTNHKFKTGLSIVADDVKEHVFNKHYNRKELVPGAFFEYTYTVTPNLS
                     LIAGIREDYHNNFGWITTPRLHVKYDATNNTIFRFAVGTGFRLANIFTENSNAFISSR
                     QFFIDNPTNNYEYGLEPEKAVNFGLSFMQKFRTNNKESFFTLDLYRTSFSQQTVVDYD
                     KNPQEIHFYNLKGKSFSNTVMAELNFYILQNLATRIAYRFLDVQTDYTTGRLQKPLQS
                     QHRAFINLEYELSKGWLVDFTTQWMSEKRIPSTTANPINKRFAAYSPNYFQMAAQITK
                     KFKSGFELYVGGENLTNYKQHHLFIDGKNPFGNYFDGSLVWGPAIGAMIYTGFRYSF"
     gene            complement(1775..3124)
                     /gene="hisS"
                     /locus_tag="PLU36_09400"
     CDS             complement(1775..3124)
                     /gene="hisS"
                     /locus_tag="PLU36_09400"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HMZ47006.1"
                     /translation="MSKISLPQGTRDFSADVVRKRNYIFNTIRTVFELYGFQPLETPA
                     MENIETLMGKYGDEGDKLIFKILNNGLDRIEKREKSAVEFQKVLEGKNNAAITERALR
                     YDLTIPFARFVAMNHGSLTMPFKRYQIQPVWRADKPQRGRYREFYQCDCDVVGSYNLL
                     NEVELANIYSTVFEKLSIDVEIKINSRKILAALAELCGGADKLIDITIAIDKLDKIGL
                     DNVKEELLQRGLNKLQITTIEKYLNITGNNKEKIIALKQLIGNNETGNKGIDELEYIL
                     SFQPKVEIDFTLARGLNYYTGIIFEVKATKVQMGSIGGGGRYDDLTGDFGVPNLPGVG
                     ISFGVDRIYDVMEELKVFPDDLKTSTEVLFFNLGMNESKTSFNLMQQLRDKGVSCELY
                     HETAKFDKQFKYADKKNIHYAVIIGSKEIEEKTCTLKNLKSGEQHSLQQNELLSFSF"
     gene            complement(3131..>3359)
                     /locus_tag="PLU36_09405"
     CDS             complement(3131..>3359)
                     /locus_tag="PLU36_09405"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HMZ47007.1"
                     /translation="LFDKIYVMDTNNYLEVKEIAGKLWNENKVKLILSELYLNENKNV
                     PDPWYGDEKDYYAVYELLDKACDAVITSLNK"
BASE COUNT         1178 a          537 c          462 g         1182 t
ORIGIN      
        1 cattacagag aatacaccag agtatagagg agttgctggc aatatgggtt ttaattttat
       61 tccaggccct tggatagaaa atattcaagt aaccaaaggt attggctctg ttgtaaatgg
      121 ctacgagagt atagctggac aaattaatgt agaattaaaa aagcctaacc aaaagcaacc
      181 tttttttatc aatggttatg taaacacaat gggcagaatg gaaggaaatt ttgttacttc
      241 caagcaattg aatgataaat ggagcactgc aactttagtg catgccaatg gtgttatagc
      301 caaaaatgac atgaacaagg atggttattt agatataccc aaaggacatc aaataaatat
      361 tgctaacaga tgggattata gcaataaaaa tgggctagga attcattgga ttttaaaagg
      421 agtttctgac agaagaattg gtggtaacaa aaattttact actgccgaca aattaactac
      481 aaacaattat ggagtaatgc taaataacga tgctttaaat gctactgcaa aaattgggta
      541 tatttttcct aaatataaac atagaagtat tggtttaata ttatctggta gttatagcaa
      601 tataaataat gtgtatggtt taactaatta taaagcaaag caaactggtt tttatagtaa
      661 cttaatttat caatctataa ttaataatac caaccataaa tttaaaactg gcttaagcat
      721 agtagccgat gatgttaaag agcatgtttt taataaacat tataatagaa aagaactggt
      781 accaggagca ttttttgaat atacttatac tgttacacct aacttatctt taatagctgg
      841 tataagagaa gattatcata ataattttgg atggattact acaccaaggt tacatgtaaa
      901 atatgatgcc acaaataata ccatttttag atttgcagtt ggtactggat ttagattggc
      961 caatattttt accgaaaact ctaatgcatt tattagctca agacaatttt ttattgataa
     1021 ccctaccaat aattatgaat atggtttaga gcctgaaaaa gcagtaaact ttggcttaag
     1081 ctttatgcaa aaatttagaa caaataataa agaaagcttt tttactttag atttatacag
     1141 aacaagcttt agccagcaaa cagttgtaga ttatgataaa aatccacaag aaattcattt
     1201 ctataattta aaaggaaaat cattttcaaa tactgtaatg gccgaactaa atttttatat
     1261 tttgcaaaac ctagctacaa gaattgcata tagattttta gatgtacaaa cagattatac
     1321 aacaggaaga ttacaaaaac cattacaatc tcagcacaga gcatttatta atttagaata
     1381 cgaattaagc aaaggctggt tggttgattt tactacacaa tggatgagtg aaaaaagaat
     1441 tccatcaacc acagctaacc caattaacaa acggtttgca gcctattctc caaactattt
     1501 tcagatggca gcacaaatta ccaaaaaatt caaaagcggt tttgagttat atgttggtgg
     1561 cgaaaatcta actaactata aacaacatca tttatttatt gatggcaaaa atccttttgg
     1621 aaattatttt gatggaagcc ttgtatgggg gccagcaatt ggtgctatga tttatacagg
     1681 atttagatat tctttttaag aaacaaattc tcttaaaaaa tacctaccat aaagtaaaat
     1741 ttggtaggta tttttttgaa aagtttaaac aagattaaaa agaaaaagaa aggagttcgt
     1801 tttgttgcaa gctgtgttgc tcacctgatt ttaaattttt taatgtacaa gttttttctt
     1861 ctatctcttt tgaaccaatg attacagcat aatgaatatt ttttttatcg gcgtatttaa
     1921 attgcttatc aaatttagca gtttcgtgat acaactcaca gctaacacct ttatctctta
     1981 attgttgcat taggttaaaa gaagttttac tttcattcat gcctaaatta aaaaataaaa
     2041 cctctgtgct tgtttttaag tcatcaggaa aaacttttaa ttcttccatt acatcataaa
     2101 ttctatctac tccaaaacta ataccaacac caggtaaatt aggaacccca aagtctccag
     2161 ttaaatcgtc atatcttcca ccacctccaa tactacccat ttgtacttta gtagctttaa
     2221 cttcaaaaat tatacctgta taatagttta agcctctggc taaagtaaaa tctatttcaa
     2281 cttttggttg aaaacttaaa atatattcaa gttcatcaat tcctttatta ccggtttcat
     2341 tattaccaat taattgcttt aatgctatta ttttttcttt attgttaccg gttatgttta
     2401 aatatttttc aatagtagta atttgaagtt tatttaatcc tcgttgcaat aactcttctt
     2461 ttacattatc taatcctatt ttatctaact tgtcaatagc tatagttata tctattagtt
     2521 tatctgcacc tccgcacaat tctgctaaag cagccaatat ttttctgctg ttaattttta
     2581 tttcaacatc tattgatagt ttctcaaaaa cagtagaata aatgttggca agctctactt
     2641 cgttcaataa attataactt cctaccacat cacaatcaca ctggtaaaat tctctatatc
     2701 tgccacgttg tggcttatca gccctccaaa caggttgaat ttgatagcgt ttaaaaggca
     2761 tggttaaact tccatgattc attgcaacaa atcttgcaaa aggtatcgtt aaatcatatc
     2821 gtaaagctct ttcggtaatg gcagcattat tttttccttc taaaactttt tgaaattcta
     2881 cagccgattt ttctcttttc tctattcggt ccaaaccatt attcaaaatt ttaaaaataa
     2941 gtttatctcc ttcatcacca tatttaccca ttaaggtttc aatattttcc attgctgggg
     3001 tttctaatgg ttgaaaacca tataactcaa atactgtacg tatagtatta aaaatataat
     3061 ttcttttcct tacaacatca gcactaaaat ctctggttcc ttgtggtaac gatattttac
     3121 tcataattac ttacttgttt aaacttgtaa taactgcatc gcaggcttta tctaatagct
     3181 catatacagc ataataatct ttttcatctc cataccaggg gtctggtaca tttttatttt
     3241 catttagata aagttcactg agtattaatt taactttatt ttcattccac aatttacctg
     3301 caatttcttt tacttctaag taattattag tatccattac ataaatctta tcaaataaa
//
LOCUS       DAOQPK010000231         3304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_20996_length_3304_cov_5.078486, whole genome shotgun sequence.
ACCESSION   DAOQPK010000231 DAOQPK010000000
VERSION     DAOQPK010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20996_length_3304_cov_5.078486"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..677)
                     /gene="fsa"
                     /locus_tag="PLU36_09410"
     CDS             complement(27..677)
                     /gene="fsa"
                     /locus_tag="PLU36_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HMZ47008.1"
                     /translation="MKFFVDTANLNQIKEANDLGILDGVTTNPSLMAKEGVKGEEAIM
                     NHYKTICEMVDGDISAEVLSVTFNEIIEEGKKLAAIHPNIVVKVPMIKDGIKAIKWFT
                     DNGIKTNCTLIFSAGQAILAAKAGATYISPFLGRIDDSGWDGVQLIEEIAQIYAVQGF
                     KSEILAASIRNPNHIIRCAAAGADVCTCPLDSILGLLKHPLTDIGLAKFLEDAKKM"
     gene            complement(764..862)
                     /gene="ffs"
                     /locus_tag="PLU36_09415"
     ncRNA           complement(764..862)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLU36_09415"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1051..2535
                     /locus_tag="PLU36_09420"
     CDS             1051..2535
                     /locus_tag="PLU36_09420"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47009.1"
                     /translation="MLHPITILLLVTIIAAIATWLLPAGTYNKLSVSEKNFVINSNGI
                     NTHVPLTQNTLDSLHIIIPLEKFTNGDITKPVSIPNTYQATKSNRTTFIQLLSSPIKG
                     IYDAIDIVLLILVMGGFIYVFNETGAMLKGISYLSFKLKGREQFLIIILTALFSFGGS
                     SYGMAEETLVFYPVLVPLFLAAGYDLLIPVAVIFAGSQLGTLSSFSNPFATIIGSNAA
                     GLNWIDGIYERILMYIISTALLIWYILRYAKKIKANPANSLVLKYDGNLHYNYSATVN
                     NEDINNTLTLRTKLLLLLFASAFIIMIAGVIFLKWWLLEITVLFFGASLLLFFINKTK
                     EAEFIQQFIKGAESLLGVAFIVGIARGITIVLNDGNISDTLLFYTAKITGSFPPAFFI
                     LILLGLFIILTLFISSSSGMAVLTMPILGALALIVHVPGREIVNSYLYGMGIMGFITP
                     TGLFLPSISMVNISTKTWFKFITPFLVILFILCAIALLIGIYIK"
     gene            2619..>3304
                     /gene="panB"
                     /locus_tag="PLU36_09425"
     CDS             2619..>3304
                     /gene="panB"
                     /locus_tag="PLU36_09425"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HMZ47010.1"
                     /translation="MSIHKEVKKITTNTLQKMKQSGEKISMLTAYDFSFAKIIDNAGI
                     DIILVGDSASNVMAGHETTLPITLDQMIYHAQSVVRAINRCLVVVDLPFGTYQSNSDI
                     ALASAIRIMKETGGHSVKLEGGAEVVESVRKIVGAGIPVMGHLGLTPQSIYKFGTYTV
                     RAKEQAEADKLLSDAKLLQEAGCFAVVVEKIPAQLAKQVSETIDIPTIGIGAGPYCDG
                     QVLVMHDMLG"
BASE COUNT         1118 a          517 c          508 g         1161 t
ORIGIN      
        1 gcttttaaat tatagtaaga aattaattac atttttttag catcttctaa aaacttggct
       61 aagccaatat cagttaaagg atgttttaat aaccctaaaa tagaatctaa cgggcaagta
      121 caaacatctg caccagcagc agcacaccta ataatatgat ttgggttacg aatagatgct
      181 gccaatattt cactcttaaa cccttgtaca gcatatattt gagcaatttc ttctattagt
      241 tgaacaccat cccaaccact atcatcaatt cttcctaaaa aaggagaaat ataagtagca
      301 ccagcttttg ctgcaagtat agcttgccca gcactaaaaa ttaaagtgca gtttgtttta
      361 ataccattat ctgtaaacca tttaattgct ttaataccat cttttatcat tggtacttta
      421 acaacaatgt taggatgaat agcagctaat tttttacctt cttcaataat ttcattaaat
      481 gtaacactca acacttctgc actaatatca ccatcaacca tttcgcaaat agttttataa
      541 tggttcataa tagcttcctc gccttttaca ccctctttag ccattaaaga agggttagta
      601 gtaactccat ctaaaatacc taaatcattt gcttctttta tttgattaag gtttgcagta
      661 tcaacaaaaa atttcatagc tttttattaa attaattgaa aaattattct ttgcaaatta
      721 tccgtattct aataaaatat atttagaatt tggtagaaga aaaaagtggc aggctactca
      781 catcgcaccg ctcaaccgcc tatccttgct gtattcctac cctggggagg ttcagcagga
      841 gctggtcgta taagacctgc cgctgcaaat gtaaggtttc tgaatttcta ataaaaagaa
      901 aatcgaaaaa taaaatttct ataaaaaatg ctcccttatt ttctatggtt cctaaacact
      961 catttataat aaaagttata tggatactta tatttatttt aacattgtta aagcaatttc
     1021 aattatttat atatgatttc tagtaaaaag atgctgcatc caataacaat tttgttgtta
     1081 gtaaccatta ttgcagcaat tgcaacttgg cttttaccag caggtacgta caataaatta
     1141 tctgtatcgg aaaaaaattt tgtaattaat agtaatggca tcaatacaca tgtgccttta
     1201 acccaaaata ctttagatag cttgcacatc attattcctt tggaaaagtt tacgaatgga
     1261 gatattacaa aacctgtctc aattcctaac acttatcaag ctacaaaaag caatagaaca
     1321 acttttattc aattattatc ttctcccata aaaggcattt acgatgctat agatattgta
     1381 ttgctaattt tagtaatggg tggatttatt tatgtattta acgaaacagg tgccatgcta
     1441 aaaggcatat cctacttatc atttaaatta aaaggaagag aacaattttt aataattatt
     1501 cttacagctt tgttttcatt tggaggctcc tcttatggta tggcagaaga aacattagtt
     1561 ttttatcctg tattagttcc tttattttta gctgctggtt acgatttatt aatacctgtt
     1621 gctgtaattt ttgcagggtc tcaattaggt acactttctt cttttagcaa tccttttgct
     1681 actataattg gctctaatgc tgcaggctta aactggattg atggcattta cgaaagaatt
     1741 ttaatgtaca ttatttctac tgctttatta atttggtata ttttaagata tgctaaaaaa
     1801 ataaaagcaa atcctgcaaa ctcattagtg ttaaaatacg atgggaacct acattataat
     1861 tattcagcaa cagtaaataa tgaagatata aacaacacac ttaccttaag aacaaaacta
     1921 ttattactac tttttgcaag tgcattcatc attatgattg caggagtaat atttttaaaa
     1981 tggtggttgc ttgaaattac agtattattt tttggagcat ccttattatt attttttata
     2041 aataaaacaa aggaagccga atttattcaa caatttataa aaggagccga aagccttttg
     2101 ggtgttgctt ttattgtagg tattgcacgt ggaattacta ttgttttaaa tgatggaaat
     2161 attagcgata cattgttatt ttacacagca aaaattactg gcagttttcc tcctgctttc
     2221 tttattttaa tattgttagg gttgttcata attttaactt tattcatttc atcatcatct
     2281 ggtatggctg ttttaactat gccaatttta ggggcacttg ctttaatagt tcatgtacca
     2341 ggaagagaaa ttgtgaatag ttatttatac ggtatgggaa taatgggctt tattacccca
     2401 acgggtttat ttttaccatc tataagcatg gtaaatatta gtacaaaaac ttggtttaaa
     2461 tttataacac cttttttagt tattcttttt attctatgtg caatagcact gttaattggt
     2521 atttatatca aataactatt cttcaataat tagaattaga aacaaaactt taattttaca
     2581 acgttaaaag ctattcctta tcttcttaaa aattttaaat gtccattcat aaagaggtta
     2641 aaaaaatcac taccaataca ttacaaaaaa tgaagcagtc tggcgaaaaa atttctatgc
     2701 ttactgctta cgatttttcc tttgcaaaaa ttattgacaa tgcaggaata gatattattt
     2761 tggttggcga tagtgcaagc aatgtaatgg ctggtcatga aacaactttg ccaataacct
     2821 tagaccaaat gatatatcat gcacaaagtg tagtaagagc aattaacaga tgtttggttg
     2881 tggttgattt accttttggt acctaccaaa gtaatagtga tattgctttg gcatctgcca
     2941 ttagaattat gaaagaaaca ggaggacatt cagttaaact tgaaggaggt gcagaagttg
     3001 tagaaagtgt tagaaaaatt gtaggtgccg gtattcctgt tatggggcat ttgggtttaa
     3061 cacctcaaag tatttataaa tttggaacct atactgtgag agccaaagaa caagcagaag
     3121 cagataaatt attaagcgat gcaaaacttt tacaagaggc tggttgcttt gcagtagttg
     3181 tagaaaaaat acctgcacaa ttagcaaagc aagtttcaga aacgatagat ataccaacta
     3241 taggtattgg tgcaggccct tattgcgatg gacaagtatt agtaatgcat gatatgcttg
     3301 gaat
//
LOCUS       DAOQPK010000232         3300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_21051_length_3300_cov_4.416025, whole genome shotgun sequence.
ACCESSION   DAOQPK010000232 DAOQPK010000000
VERSION     DAOQPK010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21051_length_3300_cov_4.416025"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2167)
                     /locus_tag="PLU36_09430"
     CDS             complement(<1..2167)
                     /locus_tag="PLU36_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HMZ47011.1"
                     /translation="MKRSITKVAVLGSGVMGSRIACHFAGIGVQVLLLDIQPKKEEGK
                     KHEPNKIVNDALATALKSNPSPIYTADVVKKITTGNFEDNMKDIATCNWIIEVVIERL
                     DIKQGVYEKVEQFRKPGTLITSNTSGIPIHLLSQGRSDDFKKNFCGTHFFNPPRYLRL
                     LEVIPTPDTDPSVVDFLMHYGDLFLGKTTVLCKDTPAFIANRIGVFSIMSIFHLMDKI
                     GLSIDEIDALTGPIIGRPKSATFRTADVVGIDTLVKVAKGVADNCPNDEAKEIFTIPS
                     WLDKIVSNNWLGDKTKQGFFKKVKTPEGKSDIQTLNLSTFEYEPRKKPKFATLETAKP
                     IDDLKTKLKAVNAGTDKAGEFYRQFHYALFSYISHRIPEISDEIYRVDDAMMAGFGWE
                     IGAFESWDVLGVAKMVELMKANGNKVATWVEEMLVNGFTSFYKVENGKRLYYDIASKK
                     YKTIPGADAFIVLSNNADKLVWKNSACRMYDIGDGVAGLEWSTKMNSIGGEVLEGLNK
                     AITLSEEKFKGLVIANDGANFSAGANVGMIFMLAIEQEYDELDMAIRMFQNSMMRLRY
                     SSIPVVTAPHGLTLGGGCEMNLHADKICAAAETYIGLVELGVGLIPGGGGTKEFVLRA
                     SDELHKDEPETNTLKDRFFSIATAKVATSAAEAFTMGILRKGQDEVVMNMGRRIAEAK
                     KSVIELFDQGYVMPQQRKDIKVLGRLGLGAMLAGINGMQR"
     gene            complement(2286..2711)
                     /locus_tag="PLU36_09435"
     CDS             complement(2286..2711)
                     /locus_tag="PLU36_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47012.1"
                     /translation="MLNVEDDVVLDPYHYNEIATISNKFLTGMGCCSINDVAPLLTFI
                     LSTDYKKEFSKQEVIIKLKSFFEQRIGFQNFSHTIENIDFDINEGMDGYGLADGIVNY
                     NTTLDSAIINSENIHFKLFFIYEEKNWKIFFFKFPEFIW"
     gene            complement(2749..>3300)
                     /gene="map"
                     /locus_tag="PLU36_09440"
     CDS             complement(2749..>3300)
                     /gene="map"
                     /locus_tag="PLU36_09440"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HMZ47013.1"
                     /translation="LKEGDVVSIDLGVILNGWHGDHAYTFILGETNNEVLQLVKVTKE
                     SLYKGIEKAIVNNRIGDIAFAIQEHTEKKHGYGVVRELVGHGLGRSLHEDPQIPNYGK
                     RGEGPKLKENLVIAIEPMINMGTKDVFTDEDGWTVRTKDGKPSVHFEHDVCVKRNQAL
                     ILSDYTFIEAAEKANTNLNTSYY"
BASE COUNT         1028 a          660 c          401 g         1211 t
ORIGIN      
        1 cacgttgcat accattaata cctgctagca ttgcacccaa acccaatcgc cctaatactt
       61 taatatcttt tctttgctgt ggcattacat aaccttggtc aaacaattca attacagatt
      121 tttttgcttc tgcaattctt ctacccatgt tcattactac ttcatcttgt ccttttctta
      181 atatacccat tgtaaaagct tctgcagcac ttgtagctac ttttgcagtt gctatagaaa
      241 aaaatctatc ttttaaggta ttggtttctg gttcatcttt atgtagttca tcacttgctc
      301 ttaaaacaaa ttctttagtg ccaccaccac cgggaattaa gcctacacct aactcaacca
      361 aaccaatata agtttctgca gcagcacaaa ttttatcagc atgtaaattc atttcgcagc
      421 ctccgcctaa tgttaaacca tgtggagctg taacaacagg aatactgcta taacgcaaac
      481 gcatcatgga attttgaaac attcttatag ccatatccaa ttcatcatat tcttgctcta
      541 tagctagcat aaaaatcatt cccacatttg cacctgcact aaaatttgca ccatcattgg
      601 ctataacaag ccctttaaat ttttcttcag ataaggttat ggctttattc aatccttcta
      661 aaacctcacc tccaatacta ttcatttttg tactccattc taagcctgca acgccatcac
      721 caatatcata cattctgcaa gcactatttt tccaaactaa cttatctgca ttattactta
      781 atacaataaa agcatcggca ccaggtatgg ttttatattt tttagatgca atatcataat
      841 acaaacgctt accattttct actttataaa acgaagtaaa tccgtttact aacatttcct
      901 caacccatgt agcaacctta ttaccattgg ctttcatcaa ttcaaccatt ttagctacac
      961 ctaaaacatc ccagctttca aatgctccaa tttcccagcc aaagccagcc atcatagcat
     1021 catctactct gtatatttca tcacttattt ctggaatacg atgagagata taagaaaata
     1081 aagcataatg aaactgcctg taaaattctc ctgctttatc tgtacctgca tttactgctt
     1141 ttaatttcgt ttttaaatca tctattggtt tagcagtttc aagtgtagca aattttggtt
     1201 tttttcttgg ttcatattca aacgtagaaa gattgagagt ttgaatatca ctttttcctt
     1261 caggcgtttt tactttcttg aaaaaacctt gtttagtttt atctcctaac caattattgc
     1321 ttacaatttt atctaaccaa cttggtattg taaatatttc tttagcttca tcattagggc
     1381 agttatctgc aacacctttg gcaactttta ccaaagtatc aattcctact acatcggctg
     1441 ttctaaaagt tgctgacttt ggacgaccaa ttattgggcc agttaatgca tctatttcat
     1501 ctatacttaa accaatttta tccattaaat gaaaaatgct cataatgcta aatacaccta
     1561 ttctattagc tataaacgca ggtgtatctt tacacaatac ggtagttttt cctaaaaaca
     1621 aatcgccata atgcattaaa aaatcaacca cagatgggtc tgtatcgggt gttggaataa
     1681 cttctaataa tcttaaataa cgtggtggat taaaaaagtg tgtgccgcaa aaattctttt
     1741 taaaatcatc acttcttcct tgtgataata aatgaatagg tatgcccgat gtattggaag
     1801 taattaatgt acctggtttt ctaaactgtt ctactttttc atacacccct tgtttaatat
     1861 caagcctttc tattaccact tcaataatcc aattgcaagt agcaatatct ttcatgttat
     1921 cctcaaagtt tcctgtagta atttttttta caacatctgc agtataaata gggcttggat
     1981 tactttttaa tgcagttgct aatgcatcat ttacaatttt attaggttca tgttttttac
     2041 cttcttcttt tttaggttgt atgtctaata acaaaacctg tacaccaata cctgcaaaat
     2101 gacaagcaat tctactaccc attaccccac tacctaaaac ggcaactttt gttatacttc
     2161 ttttcatact atttttttat acattttttc aaaaagaaat tattctattt gtatgaaaac
     2221 aaagctaagg aaaaaatagt tagctatgca actattttct ttttatacat aaaagatgct
     2281 gaattttacc aaataaattc tggaaattta aaaaagaata ttttccaatt cttttcctcg
     2341 taaatgaaaa atagtttaaa gtgaatattc tctgagttta ttattgcact atctagcgtt
     2401 gtattatagt ttacaatacc atcagccaaa ccatagccat ccattccttc attaatatca
     2461 aaatcaatat tttcaattgt gtgagaaaaa ttttgaaagc ctatgcgttg ttcaaaaaaa
     2521 gatttcaatt ttattattac ttcttgtttt gaaaattctt ttttataatc tgtagaaaga
     2581 ataaatgtta gcaaaggggc aacatcattt atactacagc aacccatacc agttaaaaat
     2641 ttatttgaaa tagtggctat ttcattataa tgataagggt ctaaaacaac atcatcctct
     2701 acatttagca ttttatatta ggcttttatg ctggtagcaa aattgaaatt aataataact
     2761 tgtatttaag ttagtatttg ctttttcagc agcttcaata aaagtatagt cagatagtat
     2821 taaagcttgg tttcttttta cacaaacatc atgttcaaag tgtacagaag gcttaccatc
     2881 tttggttctt acagtccagc catcttcatc agtaaacaca tcttttgttc ccatatttat
     2941 cattggttca attgcaatta ctaaattttc tttcagcttt ggcccttcgc ctcttttccc
     3001 ataattagga atttgagggt cttcgtgtaa acttcttcct aaaccatgtc caaccaattc
     3061 tctaacaact ccatagccat gttttttttc tgtatgttct tgtattgcaa atgcaatgtc
     3121 gccaattctg ttgtttacaa tagctttttc aattcctttg tagaggcttt ctttggtaac
     3181 ttttacaagt tgtaacactt cgttgttagt ttctcctaaa ataaaagtat aagcatgatc
     3241 tccatgccat ccatttaata ttacaccaag gtcaatagat actacatccc cttcttttaa
//
LOCUS       DAOQPK010000233         3254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_21569_length_3254_cov_3.609878, whole genome shotgun sequence.
ACCESSION   DAOQPK010000233 DAOQPK010000000
VERSION     DAOQPK010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21569_length_3254_cov_3.609878"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1806)
                     /gene="mrdA"
                     /locus_tag="PLU36_09445"
     CDS             complement(<1..1806)
                     /gene="mrdA"
                     /locus_tag="PLU36_09445"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HMZ47014.1"
                     /translation="MSTYNQSRSRVIQIIFLLVFATIIGQLINLQIFSSKYRIMADNN
                     AIYRKVIYPDRGIIYDIKKRAILENTIMFDLMVIPSDTKGIDKVAFSNLLGIDTVEYR
                     KRITTAIIKNTSVKASVFEALLTPEMYARLNENIYKFPGFMLQERSIRSYPYNTAAAI
                     LGYIGEVDTSFLRKYKDEGYEMGDYTGLNGLERTYEKVLMGQRGIKRFIRDNKGRLQG
                     SFEKGLFDTVAIAGRNLYTSIDIEVQQLAEKLLQNKIGSAVAINPKTGSIIAMATSPS
                     YNPNSLTGSTRRKNFGKLLLDTARPLLNRAIKGQYPPGSTFKPLGGLVALDENIITPN
                     YGFPCGGAYYNCGKAVRCTHSGGGHASNLRNAIAYSCNSYFVQVFRMAVDNPLYANPQ
                     KGYLKWKEYMNNFGMGVKLGIDLPGEVKASIPDTNRYNKDFGGPRWNSCNILTLGIGQ
                     DRMTATPLQLSNLMCIIANKGYYYTPHLVDSLENETIDDTVYLSKYRTKHLVTHIADS
                     IYSIIQDGMEDVTIFGTAGRLKVPGHKYCAKTGTAQNPHGNDHAVFTAFAPKENPRIA
                     VAVVVENSGYGGTWAGPVATLLMEKYLNDTLTVESE"
     gene            complement(1923..>3254)
                     /locus_tag="PLU36_09450"
     CDS             complement(1923..>3254)
                     /locus_tag="PLU36_09450"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HMZ47015.1"
                     /translation="VLQYEQTNGGNTTTFFKHRYPIATYLIAFAVSNYSVQQIKMKIG
                     IDTLQYVSYIYPESVADFQNLIIPTINALQLFSNTFGTYPFIKEQYGITQFGWGGGME
                     HQTNSFLSAPDTNLMVHELAHQWFGNKVTCHSWQHIWLNEGLATFAANYYFEKVDSAK
                     LLSFLTTQLNNITKLPDGKIIVSDTFNVGSIFNSRLSYDKAAFMLRMLRYTLGDSIFF
                     KAVFNYINDPLLQYNFVTTNHFIQHVEEVAKQDLSWFFNQWLNGEGFPTFNVSWSNNN
                     FNWVKVIVNQTTSHSSVPFYKTVLPLTFKNNHQQKTIKVFCTKNNQEFWAYLGFKADT
                     VLVDEDLQLISKSNTTIQLPLTIASDNNVLLYPNPVKDNLFLQITEPLAQQLNIQIYN
                     AFGSMVYSNLFKNISNNTLIKIPFSSYLKGFYVVKIQTETGLKGIKKIIKY"
BASE COUNT         1066 a          548 c          459 g         1181 t
ORIGIN      
        1 ttcactttcc actgttaagg tatcattcaa atatttctcc attaataaag tagctactgg
       61 gccagcccat gtacctccat aacctgagtt ttctactact actgcaactg ctattctagg
      121 attttctttt ggagcaaaag cagtaaacac agcatggtcg ttaccatgtg gattttgagc
      181 tgttcctgtt tttgcacaat acttatgacc aggaacttta agccttcctg cagtaccaaa
      241 aatggtaaca tcttccatac catcttgtat aatgctatat atgctatctg caatatgagt
      301 aactaaatgt ttggttctat acttagatag gtaaacagta tcatctattg tttcattctc
      361 taaactatct accaaatgtg gtgtataata atagcctttg ttggcaataa tgcacattaa
      421 attgcttaat tgtaaaggtg ttgcagtcat tctatcttga ccaattccta aggttaaaat
      481 attacaactg ttccaacgag gtccgccaaa atctttatta tatctattgg tatctggtat
      541 gctggcttta acctcaccag gtaaatcaat acctagcttt acacccattc caaagttgtt
      601 catatattct ttccatttta agtacccttt ttgagggtta gcatataaag gattatccac
      661 agccattcta aacacttgca caaagtatga attgcaagaa taagcaatag catttcttaa
      721 attagaagca tgtcctccac cactatgtgt acaacgaact gctttaccac aattataata
      781 tgcaccacca catgggaaac catagtttgg agtaataatg ttttcatcta aagctactaa
      841 tccacctaat ggtttaaatg ttgagcctgg aggatattgc cctttaattg ccctgtttaa
      901 taatggcctg gcagtatcta ataaaagttt tccaaaattt tttcttcttg ttgaacctgt
      961 taaagaatta ggattgtaac ttggacttgt tgccattgca attatgctac cagttttagg
     1021 gtttatggca actgcactgc caattttatt ttgtaacaat ttttcggcta attgttgcac
     1081 ttctatatca atactggtat aaagatttct acctgcaata gcaactgtat caaacaatcc
     1141 tttttcaaaa ctgccttgta atcttccttt attatctctt ataaaacgtt ttatacctct
     1201 ttgccccatc agtacttttt cataagttct ttctaaacca ttcaatcctg tataatctcc
     1261 catttcataa ccttcgtctt tatattttct taagaaagag gtatctactt cacctatata
     1321 tcctaaaatg gcagcagcag tattatatgg atagcttcta atacttcgtt cttgaagcat
     1381 aaacccagga aatttataaa tgttttcatt taatcttgca tacatttctg gtgttaataa
     1441 agcttcaaat acacttgctt ttaccgatgt gttttttata attgctgttg taattctttt
     1501 tctatactct acagtatcta ttcctaataa attgctaaaa gccactttat ctataccttt
     1561 tgtatccgat ggaataacca ttaaatcaaa cataatggtg ttttctagaa ttgctctttt
     1621 ttttatgtca taaataattc ccctatctgg gtaaattact tttctatata tggcattatt
     1681 atctgccatt attctgtatt tagaagaaaa aatttgaaga ttgattagtt gtccaataat
     1741 agtagcaaat actaataaaa aaattatttg aataaccctg cttctgcttt gattgtaggt
     1801 ggacatggta gcttaaataa tacaattttt aataaacaat actagcaaat ttgacgaatg
     1861 tatttggtag taacaataat gtacacataa aaagttattc ataaatgtta acaagcaaat
     1921 tattaatatt taattatttt ttttataccc ttcaaaccgg tttcggtttg tattttaacc
     1981 acgtaaaacc cttttaaata agagctaaat ggaattttaa ttaatgtatt attactaata
     2041 tttttaaaca aattggagta aaccatagaa ccaaaagcat tataaatttg aatatttaat
     2101 tgctgtgcta atggctctgt aatttgtaga aacaaattat ctttaacagg gttaggatat
     2161 aaaagcacat tgttatctga tgcaatagtt aagggtaatt gaatggttgt attgctttta
     2221 ctaattaatt gtaaatcttc atctactaaa acggtatctg ctttaaaacc taaataggcc
     2281 caaaactctt gattattttt tgtgcaaaat acttttattg ttttttgttg atgattgttt
     2341 ttaaatgtta atggcaatac agttttgtag aaaggaaccg atgaatgtga agttgtttgg
     2401 ttaacaataa ctttaaccca gttaaaatta ttattactcc aagaaacatt aaaagttgga
     2461 aagccttcac cattaagcca ttgattaaaa aaccaactta aatcttgctt tgctacttcc
     2521 tctacatgtt gtataaaatg attagtggtt acaaaattgt attgcaataa tgggtcattt
     2581 atataattga aaacagcttt aaaaaaaata ctatcgccta atgtatatct taacattctt
     2641 aacataaaag ctgctttgtc gtagcttaac ctagagttaa aaatactacc aacattaaat
     2701 gtgtctgata caataatttt accatctggc aacttagtaa tattatttag ttgtgtagtt
     2761 aaaaatgata aaagttttgc agaatctact ttttcaaaat agtaattagc tgcaaatgtt
     2821 gccaatcctt catttaacca aatatgctgc caactatggc aagtaacttt gttaccaaac
     2881 cattggtgtg ctaattcatg aaccattaaa ttggtatcgg gtgccgataa aaaactattt
     2941 gtttgatgct ccataccacc accccaccca aattgtgtta tgccatattg ttctttaata
     3001 aaaggataag tgccaaatgt attgctaaaa agttgcagag catttatagt aggaatgatt
     3061 aaattttgaa aatcagcaac cgactcagga tagatataag atacatattg tagggtgtca
     3121 attccaatct tcatttttat ttgttgaacc gaatagttac ttacagcaaa agcaattaaa
     3181 taagtagcaa ttggataacg gtgtttaaaa aaggttgttg tattaccacc attagtttgc
     3241 tcatattgca acac
//
LOCUS       DAOQPK010000234         3244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_21670_length_3244_cov_3.769834, whole genome shotgun sequence.
ACCESSION   DAOQPK010000234 DAOQPK010000000
VERSION     DAOQPK010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21670_length_3244_cov_3.769834"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2777)
                     /locus_tag="PLU36_09455"
     CDS             complement(<1..2777)
                     /locus_tag="PLU36_09455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HMZ47016.1"
                     /translation="MRKLVMLMACLTLFISQILAQNSRTISGKVTDENGAAISNASII
                     VKGTTIGTTTANDGTFTIKVPENAKSLIISSVNFMTVEVSISKESTVNVSLQSTSANL
                     EEVVVVGYGTQKKKEVTGAVSQVNPVNISTLVTPSVDKQLGGRATGVLVTNSSGLVNQ
                     PPRIRIRGVNSINGSSSPLFVLDGVPMESGGFAGYTSDNLLANINPADIESIDVLKDG
                     SATAIFGSRAANGVIMITTKKGKAGKLNVNYSFIQGSSKPSKKFKLLNAQEFVLITNE
                     KLTNAGQQPAAFMNNENTDTDWQSYVYRNNAKSINHNLSIDGGTDKTNFFVSFNYSDQ
                     QGLVFTNTVKRYNIRANVTQKINKWLKLSNLITLSRTEDSDQNNGGNSLSGATANVLR
                     ALPNVRVMNPSLPQFSFYNVTPDGAALGSDANTKLIENNYTNIAYVMAKNKFFSKKHR
                     VINNFGIDLKPLSWLNYSFKANIDFITLDEFLTYDPKHGDGRNSIGRVQNQAANQMRY
                     TIQNFININKSFKEHNVAVTLGHENQMQTSNSFQAIGTNISDPFFQQTNVISNSYATQ
                     QSSGSYSKGPGFVSYFGRVNYDYAGKYFVTASLRRDGLSRFAPENRFGTFPGFSVGYR
                     VSQEDYWKKSPMAKVINEFRVRTSWAKVGNAEIAGGSFPYLSLYGSAPYGAISGIAAT
                     QAGNAGLKWETNEKLGFGFDLGLLDNKINITFDYYKNNNNNLVLAAPQPPSFGVPGNL
                     IYQNIGNMENKGIELAINSKIINKKDFSWDFGINFTTQSNKVKSLYLDQDVIVSNGTG
                     NYNILQVGQPINAIYGYQFAGVNSSNGNPVYVKADGTTLVMGNITNSSYFTISDLNSN
                     VVGAASSLTGADRKVLGSSLPKWFGGITSTIRYKQFTLDMLWRFSGGNKIMNITEQES
                     LLNQAFMNSGK"
BASE COUNT         1094 a          515 c          472 g         1163 t
ORIGIN      
        1 tttttaccac tattcataaa tgcctggttc aataaagatt cttgctcggt aatattcatg
       61 attttgtttc cgcctgaaaa tctccataac atatctaaag taaactgctt ataacgaata
      121 gtgcttgtta tacctccaaa ccattttggt aaactgctac ctaaaacttt tctgtcggct
      181 cctgttaaag aagatgcagc accaacaaca ttactgttta aatctgaaat agtaaagtaa
      241 gaagagttag taatattacc cataactaaa gtagttccat ctgctttaac ataaacaggg
      301 ttaccattag atgaatttac acctgcaaac tgataaccat aaatagcatt aattggttgg
      361 ccaacttgta atatattgta gttaccagta ccattagata ctataacgtc ttggtctaag
      421 tataaagatt ttactttgtt actttgtgta gtaaagttta taccaaaatc ccaactaaag
      481 tcttttttat tgattatttt agaattaata gccaactcaa ttcctttgtt ttccatatta
      541 ccaatgtttt gataaattaa gttacctgga actccaaaag aaggtggctg aggagctgct
      601 aaaactaagt tgttgttatt atttttataa taatcaaaag tgatattaat tttattatct
      661 agtaagccta agtcaaagcc aaaacctagc ttttcatttg tttcccattt taatcctgca
      721 ttaccagctt gtgtagcagc aataccacta attgcaccat aaggagcact accatataag
      781 cttaaataag ggaaactacc acctgcaatt tctgcattac ctactttagc ccaagaagtt
      841 cttactctaa attcattaat aacttttgcc attggacttt ttttccaata atcttcttga
      901 gaaactctat aacctactga aaaacctggg aaagtcccga atctattctc tggtgcaaaa
      961 cgagataagc catctcttct tagagatgca gttacaaaat atttaccagc atagtcatag
     1021 tttacacgcc caaagtaaga tacaaatcca ggacctttac tatagctacc actagattgt
     1081 tgtgttgcat agctattact aattacgtta gtttgttgaa agaatggatc tgaaatgttt
     1141 gtaccaatag cttgaaagct atttgaagtt tgcatttggt tttcgtggcc taatgtaaca
     1201 gcaacattat gttctttaaa tgatttattt atattaatga agttttgaat ggtatatctc
     1261 atttggttag cagcttggtt ttgaacacgt ccaatactgt ttctaccatc accatgttta
     1321 gggtcgtaag ttaaaaattc atctaaagta ataaagtcaa tatttgcttt aaagctataa
     1381 ttcaaccagc ttaatggttt aagatctata ccaaaattgt ttattactct gtgtttttta
     1441 ctaaaaaatt tgtttttagc cattacataa gcaatattgg tatagttgtt ttcaattaat
     1501 ttagtatttg catcagagcc taaagcagct ccatctggtg taacattata aaaactaaat
     1561 tgaggtagag aaggattcat aactctaaca ttgggtaatg ctcttaaaac gtttgcagtt
     1621 gctccagata atgagtttcc accattgttt tggtctgaat cttctgttct tgataaagta
     1681 ataaggttac ttaatttaag ccatttgttt atcttttgag ttacgttagc acgtatatta
     1741 tatcttttaa cagtatttgt aaaaactaaa ccttgttggt ctgaataatt gaatgataca
     1801 aaaaagtttg ttttatcagt gccgccatca atagataaat tatggttaat agattttgca
     1861 ttatttctat acacataaga ttgccaatcg gtatctgtgt tttcattgtt cataaatgct
     1921 gcaggttgct gaccagcatt agttagcttt tcgtttgtaa ttaaaacaaa ttcttgtgca
     1981 tttaataatt taaacttttt agaaggttta gaagatcctt gaataaaact gtaattaaca
     2041 ttcaatttac ctgctttacc ttttttggta gttatcatta ttacaccatt tgcagcccta
     2101 gaaccaaaaa tggctgtagc actaccatct ttaagcacat ctatagattc aatatctgca
     2161 gggttaatat ttgctaataa attatcgctt gtgtaacctg caaaaccacc agattccatc
     2221 ggtacaccat ctaatacaaa tagaggggat gaactgccat taatagagtt aacaccacga
     2281 attctaattc ttggtggctg atttactaaa ccacttgaat tagtaactaa aacaccagta
     2341 gctctaccac ctagttgttt atccacacta ggagtaacta aagtagaaat gttaacagga
     2401 tttacttgac ttacagcacc tgtaacttct tttttctttt gagtaccgta accaactact
     2461 acaacttctt ctaaattggc agaagttgat tgtaggctaa cgttaacagt agattccttt
     2521 gagatagata cttctacggt cataaaattt accgaagaaa taattaatga ttttgcattt
     2581 tcaggtactt taattgtaaa agtaccatcg tttgcagtag ttgtaccaat ggttgttcct
     2641 ttaactataa tggatgcatt agaaatagct gctccatttt catcagttac ttttccggaa
     2701 atggtacgag aattttgggc aagaatctga ctaataaata atgtcagaca agccattaac
     2761 atgactaatt ttctcatttg ctttggttta aggttaagaa tggttaacaa aaagttaata
     2821 gactataaaa ataaatgaat gctgcataca aaaaataata tttttttata tgttaataag
     2881 tagttgaatt ctataaattt aagactacaa ttgagaagga atttttgaaa aataactaat
     2941 attaaaaatg aataaattat tgaattttat tgatattcaa tgtgtaatac tattctaaaa
     3001 ataaataaaa aaaaattcaa gaaaatagtt tcttaaagta tatacccatc ttactttttt
     3061 aacaaatgaa aagtgtaaat tttattttaa ttctatgtta tgcagaaagg ttacttaact
     3121 tagtttttaa ccaactcttg tttactggaa taatccaatt acttagcaaa gtatttttat
     3181 tggtaactgt attattaaaa tataatgtat ttgctgtaat aaaactattg gataatgcat
     3241 agtt
//
LOCUS       DAOQPK010000235         3159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_22668_length_3159_cov_5.673647, whole genome shotgun sequence.
ACCESSION   DAOQPK010000235 DAOQPK010000000
VERSION     DAOQPK010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22668_length_3159_cov_5.673647"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2125)
                     /locus_tag="PLU36_09460"
     CDS             complement(<1..2125)
                     /locus_tag="PLU36_09460"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HMZ47017.1"
                     /translation="MSTAIKPFLRLSGLEPLIIRPETNFVNVGERTNVTGSKKFARLI
                     KENKYEEALSVARQQVENGAQVLDVNMDDALLEGVQAMTTFLNLLQSEPDIAKIPIMI
                     DSSKFEIIEAGLKCVQGKCIVNSISMKEGVEKFIEQAIVCKDYGASVIVMAFDEKGQA
                     DTLERRISISEKAYKILTEQVGFDPQDIIFDLNVFAIATGLEEHNNYGVDFIEATRVI
                     KQKYPLVKISGGISNLSFSFRGNEPVREAMHSVFLYHAIKVGMDMGIVNAGQLVVYDE
                     IDPQLRKLCEDVILNRNNSNNEATDKLIVFAENVKAKDKTEVKDEAWRNESVEKRLAH
                     ALVNGITNYIDADTIEALEKYDKPLDVIEGPLMDGMNIVGDLFGAGKMFLPQVVKSAR
                     VMKKSVAIITPIIEQQKLNNPNEVKQSVAKILLATVKGDVHDIGKNIVGVVLGCNGYE
                     IIDLGVMVPADKILEAAVKENVDIIGVSGLITPSLDEMVHIGKEMKRRGMKQPLLIGG
                     ATTSRIHTAVKIAPEYDNGVVHVLDASRSVTVAGTLLNKDNKQEFINDTKEEYIKIKE
                     NFDNKKTVKQYISYQDALNNKVNIDWENYKPVKPSFTGVKVFEHIDLNELRSYIDWKP
                     YFIAWEMHGNFPAILTDEVVGKEATKLFNDANALLDTIINEKWLTAKAVIGFWEAESN
                     KDEVILPTQNITLQFLRQQIKKAKSQ"
     gene            complement(2261..2989)
                     /locus_tag="PLU36_09465"
     CDS             complement(2261..2989)
                     /locus_tag="PLU36_09465"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HMZ47018.1"
                     /translation="MVTLAFYNLKGGVGKTASAVNFAYLAAANGLKTLVWDLDPQGSS
                     SFYFHVKNVKNESKKIFASEMDIKESIQDSTFENLWVIPADISARNADIIFNQSRRKL
                     KSIVQGLKQFDLVILDCPPGISILHDNVFGAADWVLMPNIPTVLSIRSYETIIQYFKE
                     NDIDKAKIKCFFSMVDHRKNLHQETIQAFYNNKLFFKNYIPYLSDIEKMGVHIQPLFE
                     FANSSYAALCYRELWKEISKYCIG"
BASE COUNT          984 a          589 c          390 g         1196 t
ORIGIN      
        1 gttggctttt tgctttttta atttgttgcc ttaaaaactg taaggtaata ttttgtgtag
       61 gcaatatcac ttcgtcctta ttactttcag cttcccaaaa gccaattact gcttttgctg
      121 ttaaccattt ttcgttaata atagtatcaa gtaaagcatt agcatcatta aataattttg
      181 ttgcctcttt acctaccact tcatcggtaa gtattgctgg aaaattgcca tgcatttccc
      241 atgcaataaa atatggtttc caatctatgt agctcctaag ttcattcaaa tctatatgtt
      301 caaaaacttt aactcctgta aagcttggtt taacaggctt ataattttcc cagtcaatat
      361 taactttatt attcaatgca tcttggtagc ttatatattg ttttactgtt tttttattat
      421 caaaattttc ttttatttta atatactctt ctttagtatc attaataaat tcttgtttat
      481 tgtctttatt taaaagagtt cctgcaacag taacactacg gcttgcatct aaaacatgaa
      541 ctactccatt atcatattca ggggcaattt ttaccgcagt atgtattctt gatgtagtgg
      601 caccaccaat tagtaatggt tgtttcatgc ccctacgctt catttcttta ccaatgtgaa
      661 ccatttcatc taacgatggg gtaattaatc cactaacacc tattatatct acgttttctt
      721 taacagcggc ttctaaaatt ttatcagctg gtaccattac gcctaaatca ataatttcat
      781 acccattgca tcctaacaca acccctacaa tgtttttacc aatatcatgt acatcgcctt
      841 taacggttgc taataaaatt tttgcaacac tttgtttaac ctcattgggg ttgtttaatt
      901 tttgctgttc aataatagga gtaataatgg ctacagattt tttcattacc cttgcagatt
      961 ttacaacctg aggtaaaaac attttacctg caccaaataa gtcgccaact atattcattc
     1021 catccattag tggcccctca attacatcta agggcttatc atatttctct aaggcttcta
     1081 ttgtatctgc atcaatataa tttgttatgc catttaccaa agcatgtgct aatctttttt
     1141 caacagattc gttacgccaa gcttcatctt ttacttctgt tttatcttta gcttttacat
     1201 tttctgcaaa tacaattaac ttgtctgttg cttcattatt actattattt ctgtttaaaa
     1261 ttacatcttc acaaagtttt cttaattgtg ggtctatttc atcatataca actaattgcc
     1321 cagcatttac aatgcccata tccataccaa ctttaatagc atggtataaa aataccgagt
     1381 gcatagcttc tcttacaggt tcattacctc taaaagaaaa agaaaggtta ctaatgcctc
     1441 cactaatttt aactaatgga tatttttgtt ttataactct agtagcttct ataaaatcaa
     1501 ctccgtagtt attatgctcc tctaagcctg ttgcaatagc aaaaacattt aagtcaaaaa
     1561 ttatatcttg agggtcaaag cctacttgct ccgttaaaat tttatatgct ttttccgata
     1621 tactaattcg gcgttctaaa gtatctgcct gtcctttttc atcaaaagcc attacaatta
     1681 cagatgcacc ataatcttta caaacaattg cttgttctat aaacttttca actccttctt
     1741 tcattgaaat agaattcaca atacattttc cttgcacaca ttttaggcct gcttcaatta
     1801 tttcaaattt agaagagtct atcattatgg gtattttggc aatgtctggt tcgctttgca
     1861 ataagtttaa aaaagtagtc attgcttgta caccttccaa taaagcatca tccatattta
     1921 catctaaaac ctgtgctcca ttttctactt gttggcgtgc tacagataat gcttcctcgt
     1981 atttattttc tttaataaga cgagcaaatt ttttagagcc tgtaacattg gttctttctc
     2041 ctacatttac aaaatttgtt tctggcctaa taattaaagg ctctaatcca cttaatctta
     2101 aaaaaggttt aattgcagta ctcataaatt gctcaataaa tttcttgaag acgcaaatgt
     2161 aacgtatgct ttactgattt cttttctaaa taagaaacaa taaaaacaac accattgtat
     2221 cctccataat tactaaaaaa taaagcctgt aaattttaca ttagccaata caatatttac
     2281 taatttcttt ccataactct ctataacaaa gggcagcata gctactattg gcaaactcaa
     2341 ataagggttg aatatgtaca cccatttttt caatatcgct taaatagggt atatagtttt
     2401 taaagaataa tttattatta taaaatgctt gtatggtttc ttgatgaaga ttttttctat
     2461 ggtcaaccat actaaaaaag catttaattt ttgctttatc aatatcattt tctttaaaat
     2521 attgaataat agtttcgtaa gaacgaatgg ataaaacagt aggaatatta ggcattaaca
     2581 cccaatctgc tgcaccaaaa acattatcgt gcaaaataga aatacctggt gggcaatcta
     2641 aaataactaa atcaaattgc tttaaccctt gtacaatact ttttaatttt cttctgctct
     2701 gattaaagat aatatctgca tttcttgcag atatatcggc tggtataacc cataaatttt
     2761 caaaagtaga atcttgaata ctttctttaa tatccatttc gctggcaaaa attttcttgc
     2821 tttcattctt cacattctta acatgaaaat aaaaagaaga agacccttga gggtctaaat
     2881 cccaaactaa agtttttaac ccattggctg ctgctaaata tgcaaagttt acagcagaag
     2941 cagtttttcc aactcctcct tttaaattat aaaaagctaa agttaccata tagtacattt
     3001 ttgagattga aaaataagca tttattggct ctacacaaag tttttgatat ttaagttgta
     3061 gtatcattct ttggatctaa tgagtttcaa tttttttttt ttcatttttt gtcatcctat
     3121 tccctttttt gtcatcctga gcgtaagcga aggatctaa
//
LOCUS       DAOQPK010000236         3153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_22743_length_3153_cov_4.641382, whole genome shotgun sequence.
ACCESSION   DAOQPK010000236 DAOQPK010000000
VERSION     DAOQPK010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22743_length_3153_cov_4.641382"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..689
                     /locus_tag="PLU36_09470"
     CDS             72..689
                     /locus_tag="PLU36_09470"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HMZ47019.1"
                     /translation="MCKGTRGKSWHSETGNNIILSVVLNINWLSVRNQFLLSAWAALA
                     VFDFLSKYVQNGIKIKWTNDIYIDNSKIAGILIENKVKGNKLQWAVVGIGININQINF
                     DSTLSKATSLALVTNKKYDALLLSKELCFCLEKRFQSLINKDANGLINDYNKNLFKLN
                     EVVKLKKDNLLFNCTIKKVTKTGFLVVNNGIEQQLAFGEVEWILS"
     gene            729..1160
                     /locus_tag="PLU36_09475"
     CDS             729..1160
                     /locus_tag="PLU36_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008586651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HMZ47020.1"
                     /translation="MQTVFNLFVYGSLRKGFKNPAYNYVSRYFSLVSNNAKAKGLLFN
                     MGNFPVAKPTANNKFITGELYTIINKDEFSFAIAQLDDYEGVYAEENEKPLYYREKTI
                     VYFENVATEAWVYWFNGEVNGKQIIESGDVLQFFSEQQKGS"
     gene            1160..1915
                     /locus_tag="PLU36_09480"
     CDS             1160..1915
                     /locus_tag="PLU36_09480"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HMZ47021.1"
                     /translation="MTQATAKNIYFLSDFHLGAPNFSDSLLREKKIVAFLDSIKNNAT
                     EVFILGDMFDFWFEYKNTVPKGFTRILGKLAELTDSGIVIHFFVGNHDMWMKDYFKTE
                     LNIKVYFEPIITERFGKKLYIAHGDGLGPGDKGYKFLKKIFRNKICQWMFGILHPTWG
                     IGLANYFSRKSREKTGTADAHFLGEENEWLFIYSKEQLQKQPIDYFIFGHRHYPIKLA
                     IENSYYVNLGDWINNFTYVVLNNETLELKKWEN"
     gene            1903..2718
                     /locus_tag="PLU36_09485"
     CDS             1903..2718
                     /locus_tag="PLU36_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47022.1"
                     /translation="MGKLVFVYIIVISFYFNKSYANDTLLNLNIEQTIPGSFTNCYVD
                     NLGNIYLISATNQIKKLNSNLDSVAVFNDIKHFGTIDFIDVSNPLKILVFYKAFSTIT
                     ILDRFLSKKQTIDLRAINIQQAACIAQGYDNTIWVYDELDASIKKIDDFGKLLTTSTS
                     FRMLFDEAILPTNIIDKDGLLYLYSKNFGWLIFDYYGALKTKVMEKNWSDVQVINKTL
                     FGHNHQELLWLQPTLPLLHKRHFNINLKDVQKLIQSNGYFYTLTSKGVTIYSY"
     gene            complement(2715..>3153)
                     /locus_tag="PLU36_09490"
     CDS             complement(2715..>3153)
                     /locus_tag="PLU36_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HMZ47023.1"
                     /translation="VENNLHTVTAIEETINKTTIASWKIDDIINIERCLKVNSRIDGH
                     FVQGHVDTTAVCCEKLDINGSCTFTFSIAEKFANLIIEKGSICVNGISLTCFNVTKNS
                     FTVAIIPYTFTNTNMHSITVGNYVNIEFDVLGKYINRAKELGS"
BASE COUNT         1163 a          396 c          503 g         1091 t
ORIGIN      
        1 acttatgcca tgaaacaaat tcaggcaaaa gtggcagaac atggtacagc ctattttgct
       61 cataatcaaa catatgcaaa ggaactagag gtaaaagctg gcattctgaa acaggcaata
      121 acattatact atctgttgtt ttaaatatta attggcttag tgtacgaaat cagtttttat
      181 taagtgcttg ggctgcattg gctgtttttg attttttgag taaatatgta cagaatggca
      241 taaaaattaa atggactaat gacatttata tagataatag taaaatagca ggcattttaa
      301 ttgaaaataa agttaaaggc aataagctac aatgggctgt tgttggcatt ggaattaaca
      361 ttaatcaaat caattttgat tctactctat caaaagctac atcgctggca ttagtaacta
      421 ataaaaagta tgatgcttta ttactttcta aagaattgtg tttttgtttg gagaaaaggt
      481 ttcaatcatt aataaacaag gatgcaaatg ggttaataaa tgattataat aaaaatttat
      541 ttaagctaaa cgaagttgta aaacttaaaa aagacaactt attatttaat tgcactatta
      601 aaaaagttac taaaactggc tttttagttg taaacaatgg tattgagcaa caacttgctt
      661 ttggagaagt agagtggatt ttgagttagt aatttgtatt atataattac tgcttgtatc
      721 ttagcattat gcaaacagtt tttaatttat ttgtgtatgg ttccttaaga aaagggttta
      781 aaaatccggc ttacaattat gtgagcaggt atttttcatt agtttcaaat aatgcaaaag
      841 caaaaggatt gttgtttaat atgggcaatt ttcctgtagc caagcccaca gccaataata
      901 aatttattac aggagaactt tatacaataa ttaataaaga tgaatttagt tttgccattg
      961 ctcagttaga tgattatgaa ggtgtttatg cagaagaaaa tgaaaagcct ttatactata
     1021 gagaaaagac tattgtttac tttgaaaatg tagctacaga agcatgggtt tattggttta
     1081 atggagaagt aaacggaaaa caaattattg aaagtggcga tgtacttcaa tttttttctg
     1141 aacaacaaaa aggcagttaa tgacacaagc aactgctaaa aatatttact tcttatccga
     1201 ttttcattta ggagccccaa atttttctga cagcctttta agagagaaaa agatagttgc
     1261 ttttttagat agcattaaaa ataatgcaac tgaagttttt atattgggcg atatgtttga
     1321 tttttggttt gagtataaaa atactgtacc aaaaggcttt acaagaattt taggaaagtt
     1381 agctgagcta acagatagtg gcattgtaat acattttttt gtaggcaacc acgatatgtg
     1441 gatgaaagat tactttaaaa ccgaactaaa cattaaagta tattttgaac ctattattac
     1501 agaacgattt ggtaaaaaat tatatatagc tcatggcgat ggcttagggc caggagataa
     1561 gggctataaa tttttaaaaa aaatatttag aaataaaatt tgccaatgga tgtttggtat
     1621 tttgcatccc acatggggca tagggttagc aaattatttt agtagaaaaa gtagagaaaa
     1681 aacaggtact gcagatgctc attttttagg agaagaaaat gaatggttat ttatttatag
     1741 taaagagcaa ttacaaaaac agccaataga ttattttatt tttgggcata gacattatcc
     1801 aataaaactt gctatagaaa atagctacta tgtaaaccta ggagattgga ttaataactt
     1861 tacctatgta gtgttaaata atgaaactct tgaactgaaa aaatgggaaa actagttttt
     1921 gtttatataa ttgttattag tttttacttt aataaaagtt atgcaaacga tacattatta
     1981 aatttaaata ttgaacaaac aatacctggc agttttacga attgttatgt agataattta
     2041 ggaaacattt atttaatttc tgccaccaat caaataaaaa agctaaactc caatttggat
     2101 agtgttgctg tttttaatga tattaaacat tttggaacca ttgattttat tgatgtttcg
     2161 aaccctttga aaatattggt attttataaa gcattttcta caataactat tttagataga
     2221 tttctaagca aaaaacaaac tatcgactta agagcaataa atattcaaca agctgcatgc
     2281 attgcacaag gttatgataa tactatttgg gtttatgatg agttagatgc ctctataaaa
     2341 aaaattgatg attttggcaa actactaact acatctacca gctttagaat gttgtttgat
     2401 gaagctattt tacctaccaa cataatagat aaagatggtt tgttatactt gtatagcaaa
     2461 aactttggct ggttaatttt tgattattat ggtgctttaa aaactaaagt aatggaaaaa
     2521 aattggtcag atgtacaggt aatcaataaa actttgtttg gacataacca tcaagagctt
     2581 ttgtggttgc aaccaacctt gccattacta cataaacggc actttaatat taatttaaaa
     2641 gacgtacaaa agctaattca aagtaatggt tatttttata ccttaacatc caaaggagtt
     2701 acaatttata gttattaact gcctaattct ttagctctgt taatatactt acctaataca
     2761 tcaaactcaa tatttacata attgccaaca gtaattgaat gcatatttgt attggtaaaa
     2821 gtataaggta taatagcaac agtaaaacta tttttagtaa cattaaaaca tgtaaggcta
     2881 ataccattta cacaaataga gcctttttca attattaaat tagcaaattt ttctgcaata
     2941 ctaaaagtaa aagtacagct accattaatg tctaattttt cacagcaaac tgcagtagta
     3001 tcaacatgcc cttgtacaaa atgtccatca atcctactat taacttttaa gcatctttca
     3061 atattaataa tgtcatcaat tttccaactt gcaatagtag ttttattaat tgtttcttca
     3121 attgctgtta ctgtatgtaa gttgttttct act
//
LOCUS       DAOQPK010000237         3149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_22790_length_3149_cov_4.695540, whole genome shotgun sequence.
ACCESSION   DAOQPK010000237 DAOQPK010000000
VERSION     DAOQPK010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22790_length_3149_cov_4.695540"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /gene="glyA"
                     /locus_tag="PLU36_09495"
     CDS             <1..1001
                     /gene="glyA"
                     /locus_tag="PLU36_09495"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HMZ47024.1"
                     /translation="AALMLAILKPGDDILGLDLSMGGHLTHGSAVNFSGKLYKPHFYT
                     VTREEGLIDYEMLETKAREVKPKLIICGASAYSRDIDYARIRKVADEIGAFVMADIAH
                     PAGLIAKGLLSSPFNHCHFVTSTTHKTLRGPRGGVIMMAKDFENPFGLKDPKGNIRMM
                     SHLIDMAVFPGTQGGPLEHVIAAKAVAFGEILTNDFTLYAKQVQSNAQAMAKEFVNLG
                     YNIISGGTDNHLMLIDLRNKNISGKKAENVLGEADITANKNMVPYDDKSAFVTSGIRF
                     GVAAITTRGMKEQHMNFVVNVIDKALMNADDATILAKTKASVNEFMSQFPLYPELG"
     gene            1023..1439
                     /locus_tag="PLU36_09500"
     CDS             1023..1439
                     /locus_tag="PLU36_09500"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 family protein"
                     /protein_id="HMZ47025.1"
                     /translation="MLQRIQSVWLLIAALLSFATLQFSFFGGNKVENGISTFINYTAK
                     ENLLVLILTVAVAVAALVSVFLYKDRRQQIKIIIATLLVNILTIVLLYFNTKKFESGT
                     IALTSVVYFAVPVFLVMALLAIRKDEKLVKSADRLR"
     gene            1465..1779
                     /locus_tag="PLU36_09505"
     CDS             1465..1779
                     /locus_tag="PLU36_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HMZ47026.1"
                     /translation="MEQQQQNNNQLNIEITEEVAEGTYANLAIITHSHAEFVIDFVNV
                     MPGTPKSKVKSRTIFTPMHAKRFMKALAENIDRYEAAHGPIQDLEQIEIPMNFGGPNA
                     QA"
     gene            2363..>3149
                     /locus_tag="PLU36_09510"
     CDS             2363..>3149
                     /locus_tag="PLU36_09510"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HMZ47027.1"
                     /translation="MRKNVFNFYKKAIFTLALAMLINLHLKAQTYCSSNFTSVSFEHI
                     TNVTFAGINNTSVGTVGGPVDYTSQVATVNVGVPSTLSVTILADASEYIYAFIDWNQD
                     GDFSDSEEVYTVATSVATSGPFTLAITPPNAATPGTTRMRVMLDFAGSTPNPCKSGTY
                     GEAEDYTVNVVTSPCVAPPTAGNATSSKINACSGENFNLSLTGNSLGSGQTYKWQYSL
                     TGNAPWTDVDTAKISPSYTTTQTASTYYRCMVTCSGNSVYSDSI"
BASE COUNT         1100 a          455 c          522 g         1072 t
ORIGIN      
        1 atgcagcttt aatgttggca attttaaaac ctggtgatga tattttaggt ttagatttaa
       61 gcatgggtgg gcatttaaca catggtagtg ctgtaaattt tagtggtaaa ctatataaac
      121 cacattttta tactgttacc agagaagaag gtttaataga ttatgaaatg cttgaaacta
      181 aagcaagaga agtaaaacca aaacttatta tttgtggtgc aagtgcatac agcagagata
      241 tagattatgc aagaataaga aaagttgccg atgagatagg agcttttgta atggcagata
      301 ttgcacatcc tgcaggctta atagctaaag gtttattatc ttctccattt aatcattgtc
      361 attttgttac aagtacaaca cataaaacat taagagggcc aagaggtgga gtaattatga
      421 tggcaaaaga ttttgaaaat ccatttggct taaaagatcc taaaggaaat ataagaatga
      481 tgagccattt aatagatatg gctgttttcc caggtactca aggtggccca ttagagcatg
      541 ttattgctgc taaggcagtt gcatttggag aaatcttaac aaatgatttt acactatatg
      601 caaaacaagt acaaagtaat gcacaagcaa tggcaaaaga atttgttaac ttaggttata
      661 atattattag tggcggaacg gataatcatt taatgttaat tgatttaaga aacaaaaaca
      721 ttagtggtaa aaaagccgaa aatgttttag gagaagcaga tattactgca aataaaaata
      781 tggtacccta tgatgataaa agtgcattcg ttactagtgg tattcgtttt ggtgttgcag
      841 caattaccac aagaggaatg aaagagcaac acatgaactt tgtggtaaat gttattgaca
      901 aagctttaat gaatgcagat gatgctacta ttttagcaaa aacaaaagca agtgtaaatg
      961 agtttatgtc tcagtttcct ttatatcctg agttaggata aactttttaa aaaataattt
     1021 ttatgttaca acgtattcaa tctgtttggt tattaatagc agcattatta agttttgcca
     1081 ctctacaatt ttcttttttt ggagggaata aggtagaaaa tggcataagt acttttatta
     1141 attatacagc aaaagaaaat ttgttagtac ttatacttac agtagctgtg gctgtagcag
     1201 cattagtttc tgttttttta tataaagata gaaggcagca aataaaaatt ataattgcaa
     1261 ctttgttggt taacattctt acaattgtat tattgtattt taataccaag aaatttgaat
     1321 caggaacaat tgctttaaca tctgttgtat attttgcagt acccgttttt ttagtaatgg
     1381 ctttattagc aattaggaaa gacgagaaat tggtgaagag tgcagataga ttaagataaa
     1441 aaaataatta ttataaattt tattatggaa caacaacaac aaaacaataa tcaattaaat
     1501 attgaaatta cagaagaagt agcagaagga acttatgcca acttagcaat tataacacat
     1561 agccatgcag agtttgtaat agattttgta aatgtgatgc ccggtacacc aaaaagtaaa
     1621 gtaaaatcta gaaccatttt tacacctatg catgccaaaa gatttatgaa agcccttgcc
     1681 gaaaatattg atcgctatga ggcagcacat ggtccaattc aggatcttga acaaatagaa
     1741 ataccaatga attttggagg gccgaacgca caggcttaac tcttttgaac aatttgtagt
     1801 tttttttaaa atatagattt ttttttaaaa aaaaatagta ttattgcttt tactttgttg
     1861 aatggattaa taaagtacac atttaattgt tattttatta ttaatcaata gaatgctgct
     1921 tatgataaaa cttctacaac agttatttct gttacatcat catgtagaag gaaatttgtt
     1981 tactataaca aaacaaattt ttactttttt ttctatcgta acagtttctt ttaactcatt
     2041 catggaaaaa tggacttcta tatttattgt tgttccattt gtagcaattt ataaacagat
     2101 atcgtttaaa aaattctgga acttcaaaat acataatttc ctatatccca ttgaatgggt
     2161 taatgttcca atacctcctt ttcttgttgg ttaatttttt taaaagttaa ttaagatatt
     2221 tctcttttaa aaactttaat taactttaat gacaactcca attcttttaa aacaaactat
     2281 taaatattta ttcctgaaat taccttttga tttttaataa atattgttgc taatttcatt
     2341 actaaaaact aaaatttgtc ttatgcgaaa aaatgtattt aacttctaca aaaaggcaat
     2401 atttacattg gcacttgcaa tgttaataaa cctacattta aaagcacaaa catactgttc
     2461 ttctaatttt acaagtgttt cttttgaaca cattacaaac gtaacttttg caggaattaa
     2521 taatacatct gtaggtacag taggtggtcc tgttgattac acttctcagg ttgctaccgt
     2581 taacgttggt gtacctagta cactttccgt tacaattttg gcagacgctt ccgaatatat
     2641 atatgctttt atagactgga accaagatgg agatttttct gattcagaag aggtttatac
     2701 agttgcaact tctgttgcta caagtggtcc atttaccttg gcaataactc caccaaatgc
     2761 tgcaacacct ggtacaacca gaatgagggt gatgcttgac tttgccggat ccactccaaa
     2821 tccatgtaaa agtggtacct atggagaagc agaagattat actgtaaatg ttgttacatc
     2881 gccttgtgtt gcaccaccta cagcaggtaa tgccactagt tcaaaaatca atgcatgttc
     2941 tggagagaat tttaatttat ctcttactgg caattctctt ggtagtggcc aaacttataa
     3001 atggcaatat tccttaactg gcaatgctcc ttggacagat gttgatacag caaaaatatc
     3061 tccaagttat acaactactc aaacagcatc aacctattat cgttgcatgg taacttgtag
     3121 tggtaattct gtttattccg attcaatac
//
LOCUS       DAOQPK010000238         3144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_22864_length_3144_cov_4.241502, whole genome shotgun sequence.
ACCESSION   DAOQPK010000238 DAOQPK010000000
VERSION     DAOQPK010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22864_length_3144_cov_4.241502"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..2382)
                     /gene="recQ"
                     /locus_tag="PLU36_09515"
     CDS             complement(88..2382)
                     /gene="recQ"
                     /locus_tag="PLU36_09515"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HMZ47028.1"
                     /translation="MPISRKAATKKTTTAKKTIKRPVLENNRLPKKKYEFDLQEGLQK
                     YFGFTEFKGTQEKAIKSLLAGDDTFVIMPTGGGKSLCYQLPAMLMPGCAIIVSPLIAL
                     MKNQVDLVRGYSEQDDVAHFLNSSLNKGQIKIVKDDLLSGATKLLFVAPETLTKEENL
                     DFFSDLNISFFAVDEAHCISEWGHDFRPEYRRLREMMDVINPHIPVIALTATATPKVQ
                     SDIIKNLGLREPNIFISSFNRTNLYYEIQPKGKKEQTVKHIVKFIASHKGKSGIIYTL
                     NRKTTEELAELLVANNIKAVAYHAGLDQKVRAKGQDQFLNEDVQVIVATIAFGMGIDK
                     PDIRFVIHFNIPKSIENYYQETGRAGRDGLEGICVLYYSHKDVSKLEHLMRDKPLSER
                     EVGAQLINETVAFAESSVCRRKILLHYFGEEWGDRKCGNCDNCRNPKEKIEAKEDVVK
                     ALKVIEALDERFVADYTINVIMGKLTPQITMFRHDKSPVFGIGKDKDTLFWNSLLRQM
                     MLENLITKDIEEYGLIKFTEKGLEWKKKPSSFKIVINNIFHDTDDEDENIEMGEGTGA
                     GADDALFEMLKELRHKEAKKKGLPPFVIFLENSLQDMSTLYPTTLDELEKCQGVSKGK
                     ALKYGRPFVELIAKYVEENDIEKPDDFVMKSVANKSSNKIFIIQNVDKKIPLETIASI
                     KGLTLNELLEEMETIAASGTRLNLDYAIDDWLDEIDQEEILEYFKSCETSSLEIAQKE
                     LAEYNYNWEQLKIMRIKFLSVYGN"
     gene            2548..>3144
                     /locus_tag="PLU36_09520"
     CDS             2548..>3144
                     /locus_tag="PLU36_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HMZ47029.1"
                     /translation="MSNGNIIKIALETIELEGQSVLGLKKFIDSNFENAVNIIHQAKG
                     RVVITGIGKSAIIAQKIAATFNSTGTPSLFMHAADAIHGDLGMLQKEDVAIIISKSGE
                     SPEIKVLVPLIKNFGNTIISIVGNIESFLALQSNIILNTSVSKEACPNNLAPTSSTTA
                     QMVMGDVLAVSLMYLNGFTGKDFAKYHPGGNLGKRLYLR"
BASE COUNT          962 a          541 c          445 g         1196 t
ORIGIN      
        1 ttaaggttgt aattaaatat ttagaaatat aaataaattt caaaacttgt aattttttta
       61 aagtatataa aaaggaagtt ttaaccatta atttccatac acacttaaaa atttaattcg
      121 cattatttta agttgctccc aattataatt atattctgct aattcttttt gagctatttc
      181 aaggctcgat gtttcgcaac ttttaaagta ttctaaaatt tcttcctggt caatttcatc
      241 taaccaatca tctatagcat aatctaaatt gagccttgtg ccacttgctg caatagtttc
      301 catttcttct aaaagttcat taagtgtaag ccctttaata cttgcaatag tttctaacgg
      361 aattttttta tctacatttt gtataataaa aattttattg ctgcttttgt tggcaacact
      421 tttcatcaca aaatcatctg gtttttctat atcattttct tctacatatt ttgctattaa
      481 ttctacaaat ggccttccat attttaatgc ttttccttta ctaacccctt ggcatttttc
      541 taactcatct aaagtagttg ggtacaaggt actcatatct tgtaaggagt tttctaagaa
      601 aattacaaaa ggtggcagcc ctttcttttt tgcttcttta tgtctaagtt ctttaagcat
      661 ttcgaataaa gcatcatctg cccctgcacc agttccttct cccatctcaa tattttcatc
      721 ctcatcgtct gtatcatgaa atatattgtt tattacaatt ttaaaggagc tgggtttttt
      781 cttccactct agtccttttt ctgtaaactt tattaatccg tattcttcaa tatcttttgt
      841 aataaggttt tctaacatca tctgccttaa caaactattc caaaacaaag tgtctttatc
      901 ttttccaatt ccaaatacag gagatttgtc gtgtctaaac atagtaattt gcggagttaa
      961 tttacccata attacattga tggtgtaatc agcaacaaat ctttcatcta aagcttctat
     1021 tacttttaat gcttttacta catcttcttt tgcttcaatt ttttctttag ggtttctaca
     1081 attatcgcag ttgccacatt ttctatcccc ccattcttcg ccaaaataat gtaataaaat
     1141 ttttcgtcgg caaacactgc tttcagcaaa agcaactgtt tcattaatta gctgtgcccc
     1201 tacttctctt tcacttaaag gtttatctct cattaggtgt tctagttttg aaacatcttt
     1261 atgagaataa tacagcacac aaattccttc taaaccatct ctgcctgcac gtcctgtttc
     1321 ttgataataa ttttctatag attttggaat attgaaatga attacaaacc taatgtctgg
     1381 cttatcaata cccattccaa aagctattgt tgccacaatt acttgaacat cttcgttcaa
     1441 aaactgatct tgtcctttag ctctcacttt ttggtctaag ccagcatggt atgcaacagc
     1501 tttaatatta ttggcaacta aaagttctgc taattcttcg gttgtttttc tatttaaagt
     1561 gtaaataatt ccgcttttac ctttatgtga ggcgatgaat tttacaatgt gttttacagt
     1621 ttgttctttt tttcctttag gctgaatttc gtaatataaa tttgtacgat tgaaagatga
     1681 aataaatata ttgggttctc ttaatcctaa gttttttata atatcacttt gcactttagg
     1741 tgttgcagtt gcagttaaag caattacagg aatatgagga ttaattacat ccatcatttc
     1801 tctcaacctt ctgtattctg gtctaaaatc atgcccccat tcacttatac agtgtgcttc
     1861 atctactgca aaaaaagaaa tattcaggtc gctaaaaaaa tcaaggtttt cttcttttgt
     1921 taatgtttct ggtgcaacaa aaagtaactt ggttgctcca gaaagtaaat catcttttac
     1981 aatttttatt tgccctttat ttaaggagga gtttaaaaaa tgtgctacat cgtcttgttc
     2041 actatagcct ctaactaagt ctacctggtt tttcattaat gcaataagag gcgatacaat
     2101 aatagcacaa ccaggcataa gcattgctgg cagttgatag cataaacttt tgccaccacc
     2161 tgttggcata attacaaaag tatcatcgcc tgcaagtaag cttttaattg ctttttcttg
     2221 cgttccttta aactctgtaa agccaaaata tttttgtaac ccttcctgta agtcaaactc
     2281 atactttttt ttgggtaaac gattgttttc caaaactggt cgcttaatag ttttttttgc
     2341 agttgtggtt ttcttggtag ctgcttttct tgatatgggc ataataagtg ttcttttcct
     2401 ctttatctat aaatctttaa aggttaaaag atacaattta ttcttattac agaaataaaa
     2461 atttatagta aatttttaaa gggtagcgaa gttaatgaaa ttagtattga attacaacag
     2521 taagttgtta aactttacct ttgacaaatg agtaacggaa atatcataaa aatagcttta
     2581 gaaaccattg aactagaagg tcaatcggtg ttaggcttaa aaaaatttat tgattcaaat
     2641 tttgaaaatg cagttaatat aattcatcag gcaaaaggca gggtagttat tacaggcatt
     2701 ggcaaaagtg ctattatagc tcaaaaaatt gcagctactt ttaattctac tggcacacct
     2761 tctttattta tgcatgcagc cgatgctata catggcgatt tgggtatgtt acaaaaagaa
     2821 gatgttgcca ttattattag taaaagtggc gaaagccccg aaataaaagt actggttccg
     2881 ctaattaaaa attttggtaa tactataatt tcaattgttg gtaatataga aagttttctt
     2941 gcattacaga gtaatattat attaaatact tctgttagta aagaggcttg ccctaataat
     3001 ttggctccca caagtagtac tacagcacaa atggtaatgg gagatgtatt ggctgtttct
     3061 ttaatgtact taaacggatt tacaggaaaa gattttgcta aatatcaccc aggcggaaac
     3121 ttaggtaaaa gattatattt aaga
//
LOCUS       DAOQPK010000239         3141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_22904_length_3141_cov_4.497408, whole genome shotgun sequence.
ACCESSION   DAOQPK010000239 DAOQPK010000000
VERSION     DAOQPK010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22904_length_3141_cov_4.497408"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..634
                     /locus_tag="PLU36_09525"
     CDS             14..634
                     /locus_tag="PLU36_09525"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HMZ47030.1"
                     /translation="MYLAKTYWWLKALYPTFIWNKSREEKVIYLSFDDGPNPTVTPFV
                     LDLLKQYKAKATFFCIGKNVVHFPALYHQIINEGHSVGNHTQHHLNGWQTKTEKYIHN
                     IQTAALQIQSNLFRPPYGRIKRAQANKLTKNSSNPFQIIMWDVLSGDFDTNISGEQCF
                     INVTNNTENGSIIVFHDSDKAWERLSYTLPKILELYSKQGFEFKAL"
     gene            complement(656..988)
                     /locus_tag="PLU36_09530"
     CDS             complement(656..988)
                     /locus_tag="PLU36_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HMZ47031.1"
                     /translation="MNWLLLILAGIAEVSFTFCLGKAKQATGMEQIIWYVGFFIALII
                     SMLLLMKAIETIAIGTAYAVWTGIGAVGTVLIGIFVFKEPTNFWRLFFLMLLISSIIG
                     LKVVATKN"
     gene            complement(1025..1456)
                     /locus_tag="PLU36_09535"
     CDS             complement(1025..1456)
                     /locus_tag="PLU36_09535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ47032.1"
                     /translation="MLTSLFIVDDDPITVKICEMVIKNTEFASAVTHFENGQKCINFF
                     SNYFEKNLDKSKNVVMPELILLDLNMPVLDGWGFLDHYERKYANRLTETKIAILTSSI
                     NPQDFMKAQQYSNIIDFIYKPLLTNIVEELKEHEALKMYFS"
     gene            complement(1484..1972)
                     /locus_tag="PLU36_09540"
     CDS             complement(1484..1972)
                     /locus_tag="PLU36_09540"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HMZ47033.1"
                     /translation="MSVAKFSIEIDEFKSIEELDAADVKLLQLAQKIAENAYAPYSNF
                     YVGAVAMLNNNQIITGTNQENASYPVGTCAERVLLAIAATSFPNIPLKTMAISYLNKN
                     GLNNHPISPCGMCRQYLLEYEQRVKQPIRLILGGQVGKVYIIKSVADLLPLNFSADDF
                     KK"
     gene            complement(2042..>3141)
                     /gene="lepB"
                     /locus_tag="PLU36_09545"
     CDS             complement(2042..>3141)
                     /gene="lepB"
                     /locus_tag="PLU36_09545"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HMZ47034.1"
                     /translation="IPSGSMEKTLLVNDFLFVNKMAYGPKVPQTPLSFPFVHNLMPGS
                     QKPSYLKWIQLGYKRVPGYDKLHRNDVVVFNFPEGDTIINLPEYGSLVTYYSLVRQYG
                     RDAVWNRFENDIIVHPADKTDNYIKRCVAVAGDTLEVKDGIVYINKVKQPIPKFAQRT
                     FIVNATKAGLNFDDLKSNYNIDIRTDQYYLAYQDSINASFARTGMAEIIMTEEDAAKI
                     KQLKGVIKVEPAIEQYDAFAAQAYYFPFDSLHHHFSRDNYGPIYIPKAGAKIDLNTNT
                     LSLYYRAITSYEGHTLQVNGNEILVDGKPTTTYTFKYNYYWMMGDNRHNSQDSRFWGF
                     VPETQIVGRASLIWFSWDKGPRWKRLFNIIK"
BASE COUNT         1092 a          503 c          446 g         1100 t
ORIGIN      
        1 ttaatacaga tacatgtatt tagctaaaac atattggtgg ttaaaagcat tgtatccaac
       61 ttttatttgg aataaaagca gagaagaaaa agtaatatac ctaagttttg atgatggccc
      121 aaacccaaca gtaacaccat ttgtattaga tttattaaag caatacaaag ccaaagcaac
      181 atttttttgt ataggtaaaa atgtagttca ttttccagca ctttatcatc aaataataaa
      241 cgaaggccat tcagtaggta atcacacaca acaccatttg aatggctggc aaactaaaac
      301 agaaaaatat atccacaata ttcaaactgc tgccttacaa atacaatcaa atctttttag
      361 accaccctac ggtagaatta aacgagcaca agcaaataaa ttaaccaaaa attctagtaa
      421 tccttttcaa ataataatgt gggatgtttt aagtggagat tttgatacta atatttcagg
      481 cgaacagtgc tttattaatg ttaccaataa taccgaaaat ggaagtataa ttgtttttca
      541 tgatagtgat aaagcatggg aaagattgag ttatacttta cctaaaatac tggagttgta
      601 tagcaaacaa gggtttgaat ttaaagcctt gtaattgata tagtatcttt ttgctttaat
      661 tttttgtagc taccactttt agtccaataa ttgaagatat aagtagcatt aaaaaaaata
      721 atctccaaaa atttgttggc tctttaaaaa caaaaatacc aattagtact gtaccaactg
      781 ccccaatacc tgtccatact gcataggctg taccaattgc tatggtttca attgctttca
      841 ttaataagag catgcttata ataagggcaa taaaaaagcc tacataccaa ataatttgtt
      901 ccatgcctgt tgcttgtttt gctttaccta aacaaaaagt aaaactaact tctgctattc
      961 ctgctaaaat caaaagcaac caattcatag taaaaaatga gagtaaaatt gaatatagat
     1021 tgtattaact aaagtacatt tttaatgctt catgttcttt tagttcttca acaatattag
     1081 ttaataaggg tttgtaaata aaatcaataa tattgctata ttgttgtgct ttcataaaat
     1141 cttgtgggtt aatactagag gttaaaatag caatctttgt ttctgtaagc ctattagcgt
     1201 attttctttc ataatggtct aaaaaacccc acccatctaa tacaggcatg tttaagtcta
     1261 ataaaattaa ttcaggcatt acaacatttt tacttttatc taggttcttt tcaaaataat
     1321 tagaaaagaa gttgatacac ttttggccat tctcaaaatg agttacagca ctagcaaact
     1381 ctgtattttt gataaccatt tcacaaattt ttactgtaat agggtcatca tctacaataa
     1441 ataatgaagt aagcatgttg ttttgattta aaaaattaaa aaatcatttt ttaaaatcat
     1501 ctgcactaaa attaagtggc aataaatctg caacactttt aataatataa actttaccta
     1561 cttggccacc taaaattaat cttataggtt gttttactct ttgttcatac tctaataaat
     1621 actgcctgca cataccacat ggtgaaatag ggtggttgtt caatccattt ttattcaaat
     1681 aactaatagc catagttttt aatggaatat tgggaaagct tgtagctgca atagctaata
     1741 aaactctttc ggcacaagta cctacagggt aagaagcatt ttcttgattg gtacctgtta
     1801 ttatttggtt attgtttaac attgctactg cacccacata aaagtttgaa taaggtgcat
     1861 aagcattttc ggctattttt tgagccaatt gtaaaagttt tacatcggct gcatctagtt
     1921 cttctatact tttaaattca tctatttcaa tactgaattt agctacggac atacattatt
     1981 ctttttaatt aataagctat taaagaaata tataaaagct tatctgatag gaaagataag
     2041 actattttat gatattaaat aatcttttcc atctagggcc tttatcccaa ctaaaccata
     2101 ttaaggatgc tctacctaca atttgagttt cgggtacaaa accccaaaat ctgctatctt
     2161 ggctattatg cctattgtca cccatcatcc aataataatt gtatttaaaa gtataggtgg
     2221 tagtaggttt cccatctact aaaatttcat tgccatttac ttgaagagta tggccttcgt
     2281 agcttgtaat ggctctgtaa tatagagata gtgtatttgt gtttaaatca atttttgcac
     2341 cagctttagg tatataaatt gggccataat tatctcttga aaaatgatga tgtaaagaat
     2401 cgaaaggaaa ataataagct tgtgctgcaa atgcatcgta ttgttctatt gcaggttcta
     2461 ctttaataac tccttttaat tgttttattt ttgcagcatc ttcttcagtc ataattattt
     2521 cagccattcc tgttctggca aaagaggcat tgatagaatc ttgataagcc aaataatatt
     2581 gatctgttct tatatcaatg ttatagtttg attttaaatc atcaaaattt aagcctgctt
     2641 ttgtagcatt tactataaag gttctttgtg caaattttgg aatgggttgt ttcactttat
     2701 taatatacac tatgccatct ttcacttcta aagtatctcc tgcaacagct acacaacgtt
     2761 taatataatt atctgtttta tcggcagggt gaacaataat atcgttttca aatctattcc
     2821 aaactgcatc tcttccatat tgtcttacta aagaataata agtaactaac gaaccatact
     2881 ctggcaaatt aataattgta tctccttcgg gaaaattaaa aacaacaaca tcgtttctat
     2941 gtaatttatc atagcctgga actcttttgt aacctagctg tatccatttt aagtatgatg
     3001 gcttttgaga gccaggcatt aaattatgaa caaaaggaaa acttaatgga gtttgaggaa
     3061 cttttggccc ataagccatt ttgtttacaa ataaaaagtc gttaaccaat aaagtttttt
     3121 ccatactacc cgatggaata a
//
LOCUS       DAOQPK010000240         3139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_22925_length_3139_cov_5.854410, whole genome shotgun sequence.
ACCESSION   DAOQPK010000240 DAOQPK010000000
VERSION     DAOQPK010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22925_length_3139_cov_5.854410"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..559
                     /gene="rsfS"
                     /locus_tag="PLU36_09550"
     CDS             152..559
                     /gene="rsfS"
                     /locus_tag="PLU36_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HMZ47035.1"
                     /translation="MANLSVLNTRKKSTVVKLTRNSKIFKSIINAIEDKKGEHIVSLD
                     LRKIPEASADFFIICQASSTTQLKAICEFIVEEVKLKCNETPNKQEGKQALQWILVDY
                     INIVVHIMYPEARNFYKLEEMWNDAGILKHETN"
     gene            631..2730
                     /gene="ftsH"
                     /locus_tag="PLU36_09555"
     CDS             631..2730
                     /gene="ftsH"
                     /locus_tag="PLU36_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HMZ47036.1"
                     /translation="MDEQKQKNLRPNLNTGGNKSKKSPKFSIYWIYAAIIILLLSYRI
                     FDMGTPETVSTTSLEFKNEMLAKGDVSKIDQVDNAGRKSIKVYLIADSLDKPFYKEKF
                     KNATIKPKEVAKKGLPLFEFKVSDFKNFDDELSKFYSENKNIQEVPKNYKQEGEFWGP
                     IANIVLTLIIVIGSWVLLMRKMGGGSGSGGGAGGIFSIGKSKAQLFEKGTKVNINFGD
                     VAGLDEAKVEVMEIVDFLKNPKKYTALGGKIPKGALLVGPPGTGKTLLAKAMAGEAQV
                     PFFSLSGSDFVEMFVGVGASRVRDLFKQAREKAPCIIFIDEIDAIGRARGKNVMMSND
                     ERENTLNQLLVEMDGFAGDAGIIILAATNRPDVLDSALLRPGRFDRQITIDKPDVKGR
                     EAIFKVHLQPIKVSETLDVHKLAEQTPGFAGADIANVCNEAALIAARKNKAAVDMSDF
                     QDAIDRVIGGLEKKNKIIAPHEKEIIAYHEAGHAICGWFLEHAYPLLKVTIVPRGSAA
                     LGYAQYTPKEQHLYNTDQLMDQICMTLGGRAAEEIFFNKISTGAANDLQQITKIAYSM
                     VTVYGMNSKVGNISFYDPGQENAFQKPYSEETGKIIDEEVRKLIDTAYIRTKELLTEK
                     KEQVEKLAKELLDKEVLHQSDVEELIGKRPFEEKKIIDVQEHPSSENQTTTNTPTETN
                     NINVVENSVTANETNNE"
     gene            2711..>3139
                     /locus_tag="PLU36_09560"
     CDS             2711..>3139
                     /locus_tag="PLU36_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B/C"
                     /protein_id="HMZ47037.1"
                     /translation="MKQTMSDQSKNRILSKIKQALAKPVPEPFTSVKPNLQNIYTPTQ
                     QELEIEFAENFTQLSGKFSFCTNEQELANQLNHLTSSRNWNKIFCREPELKYILSLNN
                     FKNFEHNDLNTCQVSITSCEFLVARTGSIFLSSLQKSGRNT"
BASE COUNT         1249 a          448 c          559 g          883 t
ORIGIN      
        1 aataaaagta ttcccaatag ttggtaaaga aatgttagga gtgtttgatg ttggcaaaag
       61 attgttattt ttggcaaaat tagcagtaat aaaaaccatt tggttttata caggcaacat
      121 aaaaattaat taactataat aatattcatt tttggcaaat ctttctgttt taaatacccg
      181 caaaaaaagt actgttgtaa aacttaccag aaattctaaa attttcaaat ccataattaa
      241 tgcaattgaa gataaaaaag gagaacatat tgtaagttta gatttaagaa aaataccaga
      301 agcatcggca gatttcttta tcatttgcca ggcttctagt actactcaat taaaagcaat
      361 ttgcgaattt attgtagaag aagtgaagtt aaaatgcaat gaaacaccta ataagcaaga
      421 gggtaagcaa gcattacaat ggattttagt tgattatata aatatagttg tacacattat
      481 gtatcctgaa gcaagaaact tttataaatt agaagaaatg tggaatgatg caggaatatt
      541 aaaacacgaa accaattaat ttaattacaa aagccaagaa ggtcaaagaa aaaaacacaa
      601 aattcaacga ccataaacta ttatcaacct atggatgagc aaaaacaaaa aaacttaaga
      661 cctaatttaa atacaggagg aaataaatca aagaaaagcc caaagttttc gatttattgg
      721 atatacgctg ccattattat tctattatta agttatagaa tatttgatat gggtacacct
      781 gaaactgttt ctaccacatc attagaattt aaaaatgaaa tgcttgccaa aggcgatgtt
      841 tcaaaaatag accaagtaga taatgctgga agaaaaagca taaaagtata tttaatagca
      901 gatagcttag ataaaccttt ttataaagaa aaatttaaaa atgcaactat aaaaccaaaa
      961 gaagttgcta aaaaagggct accattattt gagtttaaag tatctgactt taaaaatttt
     1021 gatgatgaat tatccaaatt ttactccgaa aataaaaata ttcaagaggt tccaaagaat
     1081 tataaacaag agggagaatt ttggggaccc attgcaaata ttgttctaac acttattata
     1141 gtaattggct cctgggtttt attaatgcgt aagatgggtg gaggctctgg cagtggtggt
     1201 ggtgctggtg gtatttttag tataggaaaa agtaaagccc agctatttga aaaaggtact
     1261 aaagtaaaca tcaattttgg tgatgtagcc ggtttagatg aagccaaagt agaagtaatg
     1321 gaaattgttg acttcctaaa aaaccctaaa aaatacacag cattaggagg caaaatacct
     1381 aaaggagctt tattagttgg ccctccagga acaggtaaaa ccttattagc aaaagctatg
     1441 gctggcgaag cccaagttcc attctttagc ttaagcggta gcgattttgt ggaaatgttt
     1501 gtaggtgttg gtgcaagccg tgtaagagat ttatttaaac aagcccgtga aaaagcacca
     1561 tgtattattt ttattgatga aatagatgct ataggcagag caagagggaa aaatgtaatg
     1621 atgagtaatg acgaaagaga aaacaccctt aaccaattac ttgtagaaat ggatggcttt
     1681 gcaggagacg ctggtattat tattcttgct gcaaccaaca ggccagatgt tttagatagt
     1741 gctttattaa gaccaggcag gtttgataga caaataacta ttgataaacc agatgttaaa
     1801 ggcagagaag caatttttaa agtacaccta caacctatta aggtttctga aacattagat
     1861 gtacataagc ttgccgaaca aacacctggt tttgcaggtg ccgacattgc aaatgtttgc
     1921 aatgaagctg ctttaatagc tgccagaaaa aataaagcag cagttgatat gagcgatttt
     1981 caagatgcta ttgatagagt aattggcgga cttgaaaaga aaaataaaat aattgctcca
     2041 cacgaaaaag aaattattgc ataccacgaa gctggtcatg ctatatgtgg ttggttttta
     2101 gagcatgctt accctttatt aaaagtaaca attgtaccaa gaggttctgc agcattagga
     2161 tatgcccaat atacccctaa agaacaacac ttatataata cagatcaatt gatggatcag
     2221 atatgtatga ctttgggcgg tagagctgca gaagaaattt tctttaacaa aatatctaca
     2281 ggtgcagcga acgatttaca gcaaattact aaaattgctt acagtatggt tactgtatat
     2341 ggtatgaaca gtaaagtagg taatataagt ttttacgacc caggacaaga gaatgctttt
     2401 caaaaacctt atagtgaaga aacaggtaaa ataatagatg aagaagtaag aaaactaatt
     2461 gatactgcat acatacgcac aaaagaatta ttaacagaga aaaaagaaca agtagaaaaa
     2521 ttagcaaaag aattattaga taaagaagta cttcatcaaa gcgatgtgga ggaattaatt
     2581 ggtaaaagac catttgaaga gaagaaaatt atagatgttc aagaacatcc ctctagtgaa
     2641 aatcaaacca ctacaaatac tccaactgaa accaacaaca ttaatgtagt tgaaaattca
     2701 gttactgcta atgaaacaaa caatgagtga ccaatctaaa aatagaatat tatccaaaat
     2761 aaaacaggca ctggcaaaac cagtgcctga gccttttaca tctgttaaac ccaacctgca
     2821 aaatatatat acaccaactc agcaagaatt agaaatagaa tttgcagaaa actttactca
     2881 actttctggt aaattttcat tttgtacaaa cgaacaagaa ttagccaatc aactaaatca
     2941 cttaaccagt agcagaaatt ggaataaaat tttttgtaga gaacccgaat taaaatacat
     3001 tttaagttta aataacttta aaaattttga acataacgat ttaaacacct gtcaggtaag
     3061 cattacctca tgtgagtttt tagttgccag aactggtagt atttttttaa gcagccttca
     3121 aaaaagtgga aggaataca
//
LOCUS       DAOQPK010000241         3127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_23091_length_3127_cov_4.123047, whole genome shotgun sequence.
ACCESSION   DAOQPK010000241 DAOQPK010000000
VERSION     DAOQPK010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23091_length_3127_cov_4.123047"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PLU36_09565"
     CDS             <1..615
                     /locus_tag="PLU36_09565"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HMZ47038.1"
                     /translation="MDMVGRYDTAHKLTIGGYGTSPVWDNIFTTISNKNLILKFDSSG
                     SGPSDHASFYRANIPVLFFFTGSHSDYHKTTDDWEKINFTAQKDIIHLIYNIVDATNN
                     KGKLAFTKTREPKVGKAKFSVSLGVIPDYGYTGTGLRIEGVSPGKLAEKIGLQAGDVL
                     LQLGEYKFVDVNSYMEALSKFKKGDATKLTIKRGDKEMSFDIVF"
     gene            639..1787
                     /locus_tag="PLU36_09570"
     CDS             639..1787
                     /locus_tag="PLU36_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HMZ47039.1"
                     /translation="MNIGFEAKRAYTNNTGLGHYSRTLITSLATYFANHQYYLFTPKL
                     SNFFNTSSFQNIQTIVPTSFPATLFTAIWRSSWVKKDLKKLNIQLYHGLSHEIPLGIQ
                     HTNIASVVTMHDLIFERYQEQYNSMDVKIYRNKFKNACKNSNKIIAISKQTKADLIDF
                     YKIEEEKIAICYQSCNPIFAQQETKETLETIKQFYQLPNKYFLYVGSIIERKNLLLIC
                     KALKMLQPAIKIPLVVIGEGGAYKKIIKQFIFSNGLENDVIFLSEKYTNDSFKTAKDF
                     PAIYQQAIALIYPSIFEGFGIPVLEALWSKTPVITSNVSCMPETGGDAAMYVNPYSEM
                     EMANAMEEIYKNDSLRESMIVKGISHAQNFTQEKCASEVFKVYKQIIN"
     gene            1790..2353
                     /locus_tag="PLU36_09575"
     CDS             1790..2353
                     /locus_tag="PLU36_09575"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HMZ47040.1"
                     /translation="MNFNSDIEACLHILHNGGIILYPTDTIWGIGCDATNEDAVEKII
                     QLKQRVANKNFIVLVSDEKELMQYVAALDLEIFNYLETQKKPTTVIYHQAIGLANSVI
                     AEDGSVAIRICKDEFCKTLIKRFRKPIVSTSANISNQPSPKLFLDISNEIKNGVDYVV
                     AHKQNDQTPSTPSSIIEWKNGEIINVR"
     gene            complement(2366..2569)
                     /locus_tag="PLU36_09580"
     CDS             complement(2366..2569)
                     /locus_tag="PLU36_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47041.1"
                     /translation="MKTKLLRLTGILAIVMLIAIPVMAQDNFPDPEDDIPVDGGLTLL
                     LAAGAGLGAKKLRDKRKQNRNNI"
BASE COUNT         1147 a          476 c          489 g         1015 t
ORIGIN      
        1 atggatatgg ttggtaggta tgatacagcc cataaactta ccataggagg gtatggaaca
       61 tctccagttt gggataatat ttttaccact ataagtaata aaaatttaat tctaaaattt
      121 gatagctctg gaagtggccc tagcgaccat gccagttttt acagagcaaa tattccagta
      181 ttattctttt ttacaggaag ccattccgat tatcacaaaa caacagatga ttgggaaaaa
      241 attaatttta ctgcacaaaa agatattata cacctaattt ataatattgt tgatgcaaca
      301 aacaacaaag gcaaattagc atttactaaa acaagagaac ctaaagtggg caaagcaaaa
      361 ttctctgtta gcttaggcgt aataccagat tatggctata ctggaactgg tttaagaata
      421 gaaggagtaa gcccaggaaa attagctgaa aaaattggtt tacaagccgg agatgtttta
      481 ctacaattag gtgaatacaa atttgtagat gtaaacagct atatggaggc attaagcaaa
      541 tttaaaaaag gagacgcaac caaacttaca attaaacgag gcgataaaga aatgagtttt
      601 gatatagtgt tttaaaaaat aacttaaatc tgcttataat gaatataggt tttgaggcta
      661 aaagagccta taccaataat acaggtttag gacattatag tcgtacttta attacttctt
      721 tagcaactta ttttgctaac catcaatatt atttatttac accaaagctc agtaattttt
      781 ttaatacttc atctttccag aatatacaaa ccattgtacc cactagcttt cctgcaacat
      841 tatttacagc tatatggcgt agcagttggg taaaaaaaga tttaaaaaaa ttaaatattc
      901 aattgtatca tggcttgagt cacgaaattc ctttaggtat tcaacatact aatattgcat
      961 ctgtagttac catgcatgat ttaatttttg aaaggtatca agagcaatat aattcaatgg
     1021 atgtaaaaat ttaccgaaat aaatttaaaa atgcttgtaa aaattcaaat aaaattattg
     1081 ccataagcaa gcaaactaaa gccgatttaa tagattttta taaaatagaa gaagaaaaaa
     1141 ttgctatttg ttatcaaagc tgtaacccca tttttgcaca acaagaaact aaagaaacgc
     1201 tagaaaccat taagcaattt tatcagctac ccaacaaata ttttttgtat gtaggttcca
     1261 ttatagaaag aaagaatttg ttgctcattt gtaaagcatt aaaaatgcta caacctgcca
     1321 taaaaattcc attagtagta ataggagaag ggggagcata caaaaaaatt ataaaacaat
     1381 ttattttttc gaatggctta gaaaatgatg taatcttttt gtcagaaaaa tatactaatg
     1441 attcttttaa aactgcaaaa gattttcctg ctatatacca acaagcaatt gcgttaattt
     1501 acccatctat ttttgaagga tttggaatac ctgttttaga agcattatgg agtaaaactc
     1561 ctgtaattac aagtaatgta tcttgcatgc cagaaactgg tggtgatgct gccatgtatg
     1621 taaaccctta cagcgaaatg gaaatggcta atgcaatgga agaaatttat aaaaacgata
     1681 gtttaagaga aagtatgata gtaaaaggta tttctcatgc tcaaaacttt acccaagaaa
     1741 aatgtgcatc cgaagttttt aaagtatata agcaaataat aaattaatag tgaattttaa
     1801 ctctgatata gaagcatgct tacacatttt acataatgga ggcattattt tatacccaac
     1861 agataccatt tggggtattg gttgtgatgc tactaatgaa gatgcagtgg aaaaaattat
     1921 acaattaaaa caaagagtag caaataaaaa ttttattgta ttagtaagcg atgaaaaaga
     1981 actaatgcaa tacgtagcag ccttagattt agaaatattt aattatttag aaacacaaaa
     2041 aaaaccaaca actgttattt atcatcaagc aattggttta gcaaatagtg taattgccga
     2101 agatggctct gtggctataa gaatttgtaa agatgaattt tgtaaaacac ttattaaaag
     2161 atttagaaaa ccaatagtaa gtacatctgc caatattagc aaccaacctt ccccaaaatt
     2221 atttttagat ataagcaacg aaataaaaaa tggagttgat tatgtggttg ctcataaaca
     2281 aaatgatcaa acaccctcaa ctccatcttc aattattgaa tggaaaaatg gcgaaataat
     2341 taacgtacgt taatcattaa agtaattaaa tattatttct attttgtttc cttttatctc
     2401 ttaatttctt tgctcctaaa ccagctcctg cagctaacaa taaagttaaa ccaccatcta
     2461 caggtatatc atcttcagga tcaggaaaat tatcctgtgc cattactggt attgctatta
     2521 gcataactat tgctaaaatt cctgttagtc ttaatagctt tgttttcata tctcttttag
     2581 ttttaagtgc ttcgtagtaa aagtaaacaa cgaattataa ttaattaaat tttatttttc
     2641 tttttatgtc atgttgaata aagcgaaaca aaaaccaatg tcatgttgag caaagcgaaa
     2701 catctacttg tgcttcatta ctgctgctca ggagattctt cgcttcgctc agaatgacat
     2761 taatgaggag ctcagaatga cattaatgag gagctcagaa tgacatgaaa tgatgaatct
     2821 gtttgtgttc ttcctattgt catgttgagc aaagcaatac caaaagcact gtcatgttga
     2881 gcaaagcgaa acatctactt atgttttatt aagattcttc gcttcgctca gaatgacatg
     2941 aatgagactc agaattacat ttttattttt tagttcttta tcaatttttc tatgcttcta
     3001 ttgcctttgc tatctgttac ttctactaaa tatgtgccgg ctgttagcct gcctatattc
     3061 atttggcttg tacctgcatt ggctttgtat tgcattacta ccttgcctgc catatcggtt
     3121 acactta
//
LOCUS       DAOQPK010000242         3121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_23172_length_3121_cov_4.714938, whole genome shotgun sequence.
ACCESSION   DAOQPK010000242 DAOQPK010000000
VERSION     DAOQPK010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23172_length_3121_cov_4.714938"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..1023)
                     /locus_tag="PLU36_09585"
     CDS             complement(58..1023)
                     /locus_tag="PLU36_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47042.1"
                     /translation="MLKIIVAILLCFNVNNLFAQEFIVTTPKQKRNDFAKNFVRVLNN
                     AGNYFKDIKDKALKEEDTIFTEGNVFNNKIKLKGAVAARIIEDSTTYAGYFFGNYPNL
                     DAVEAAYVNLTNLIAEALMGKVIFRSVDGSNKTPWVKQTKIAYTQNSGFFMFNMFVEI
                     YQNNADSTLNLILKVKGGKPQYYYKIACNEPIKSFMFVSSLRTQVNTFQKRKFDGCLG
                     DIPMFNCIGERKSKDSLEVVYEKLGFKELPDALKEFEATLTNIRVSLNDYYVYYITNN
                     KNNNIKQVNFLKFDDIDIKHPKSLKLSLVEKLHKNFSLELSFVYK"
     gene            complement(1138..2187)
                     /locus_tag="PLU36_09590"
     CDS             complement(1138..2187)
                     /locus_tag="PLU36_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HMZ47043.1"
                     /translation="MKHFNFYTKQQILSYTKIRRFETKLGERIRCISNKEDFEQAIKL
                     PSVKFVLFGIPEDIGVQANYGIGGTNSVWLPFLQSFLNIQSNDFLDGAEILLLGYFDF
                     SELNKIIDINAENKDEKVEAFRHAVNTIDIEVEQLMHVITQNNKLPIVIGGGHNNAYP
                     CIKGAAKGWHKAGITPLAQINAINLDAHSDYRPMEGRHSGNGFKYAEEDGYLEKYCVI
                     GLHENYIQQNVWVDMVNNPFIDCITYEDIFIHEKKSFIQSVLHATAFTKETLTGIELD
                     IDCIQNALSSALTPVGLTPTHARQYINYAALQSKPAYLHICEGATQLNDGRVDSSTGK
                     LISYLVSDFIKAILE"
     gene            complement(2322..2675)
                     /locus_tag="PLU36_09595"
     CDS             complement(2322..2675)
                     /locus_tag="PLU36_09595"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HMZ47044.1"
                     /translation="MLSIHLNNLLFYSYHGLFHEEKTIGNEFELNITVHQLEVKENIT
                     SLDDTINYVKIYELVAIRMKQPTLLLETLCQDICRIILYNFSLANSVQINIKKKHPPI
                     SQLQGSVAVSFEMKR"
     gene            complement(2693..>3121)
                     /locus_tag="PLU36_09600"
     CDS             complement(2693..>3121)
                     /locus_tag="PLU36_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMZ47045.1"
                     /translation="STVHMIVRRDAMRASKVMQERVLATPNIKIYWNSETDEILGDNK
                     TEGVRIKNTLTNSTIDIAISGFFVAIGHQPNSKIFTGWIDMDDTGYIKTIPGSSKTSL
                     AGIFAAGDVQDKIYRQAVTAAGSGCMAALDAERYLATLEH"
BASE COUNT         1010 a          522 c          384 g         1205 t
ORIGIN      
        1 tataaaaaag ctgaacccct aattatttta cttacacact ttatgggata gtgtccttta
       61 tttatacaca aagcttaact ccaggctaaa atttttatgt aacttctcca ctaaactaag
      121 ctttagcgat ttaggatgct tgatatctat atcatcaaat tttaaaaagt taacttgttt
      181 aatattattg tttttattgt ttgttatata ataaacataa taatcattta ggctaactct
      241 aatattggta agtgtggctt caaattcttt caatgcatct ggcaactcct taaacccaag
      301 tttttcatac accacttcta acgaatcttt actttttctt tctccaatgc aattaaacat
      361 tggtatatct cctaaacaac catcaaattt tcttttttga aatgtattta cttgtgttct
      421 taatgaacta acaaacataa aacttttgat aggttcatta caggctattt tataataata
      481 ctgtggtttg cctcctttta cttttaaaat taagtttaat gtgctatctg cattgttttg
      541 ataaatttca acaaacatat tgaacataaa aaagccgcta ttttgtgtat aagcaatttt
      601 tgtttgctta acccaaggtg ttttattgct accatccaca cttctaaaga ttactttacc
      661 cattaatgct tcggcaatta aattagttaa gtttacataa gctgcttcta cagcatcaag
      721 gtttggataa ttgccaaaaa aatatcctgc ataggtagta ctatcctcaa ttattcttgc
      781 agctacagca cctttcagct ttattttatt attaaacaca ttgccttcag taaaaatagt
      841 atcttcttct tttagtgctt tatctttaat atctttaaaa taattgccag cattattaag
      901 tactcttaca aaattttttg caaaatcatt tcttttttgc tttggggtag taactataaa
      961 ctcttgtgca aacaaattat ttacattaaa acataataat attgcaacaa taattttaag
     1021 catttaataa aaaatttggc aacataaatg taagcttagt ttatagtaat tataacgaaa
     1081 ataattaaca ctatagtatt cgttgtttgc aactttagtt ttaaagtgct tcaactatta
     1141 ttctaatatt gcttttataa aatcactcac taaatagcta atcaattttc cggtactgct
     1201 atctactctt ccatcattta attgtgtagc accttcgcaa atatgtaaat aggctggttt
     1261 gctttgtaat gctgcataat taatgtattg ccttgcatga gttggagtaa ggccaacagg
     1321 ggttaaagca ctacttaatg cattttgtat gcaatctata tcaagttcaa tacctgttaa
     1381 agtttctttt gtaaaagcag ttgcatgtaa tacagattgt ataaatgatt ttttttcgtg
     1441 aataaaaata tcttcgtatg taatacaatc tataaaaggg ttgtttacca tatccaccca
     1501 aacattttgc tgaatataat tttcgtgtag gccaataaca caatattttt ctaaataacc
     1561 atcttcttcg gcatatttaa aaccattgcc actatgcctt ccttccattg gcctgtaatc
     1621 tgaatgtgca tctaaattaa ttgcattaat ttgtgcaaga ggtgttatac ctgctttatg
     1681 ccagccttta gcagcacctt ttatacaagg gtatgcattg ttatgcccac caccaattac
     1741 aattggtaat ttattatttt gagtaattac atgcattaat tgttctactt ctatatcaat
     1801 tgtatttact gcatgcctaa aagcttctac tttttcatcc ttattttctg cattaatatc
     1861 tataatttta ttaagttcag aaaaatcaaa atagcccaaa agtaaaattt cagcaccgtc
     1921 taaaaaatcg ttactctgaa tatttaaaaa agattgtaga aatggcaacc aaacactatt
     1981 agtgccacct atgccataat ttgcttgtac acctatatct tctggtatgc caaataaaac
     2041 aaattttact gatggtaatt ttatggcttg ctcaaagtct tctttattac taatacatct
     2101 aattctttcg cccaatttag tttcaaacct tctaattttt gtataagata aaatttgttg
     2161 tttggtataa aagttaaaat gcttcataag tttttttttg agctaaagca aaagtaattg
     2221 ggttgctgta atgaagctat attttagtaa tatacttgta aacaattaat tccaatactt
     2281 ttaataatta agatatattt tcaattattt acacctttat attacctttt catttcaaaa
     2341 cttacggcaa cgctaccttg caattggctt ataggtgggt gttttttctt aatattaatt
     2401 tgaacagagt tagccaatga aaaattgtat aaaataattc tacaaatatc ctgacaaagg
     2461 gtttctaata ataaagttgg ttgcttcatt ctaatggcaa ccaactcata aatttttaca
     2521 taattaatgg tatcatctaa ggaggtaata ttttctttta cttctaattg atgcacagta
     2581 atatttagtt caaattcatt tcctattgtt ttttcttcat gaaataaacc atgataagaa
     2641 taaaacaata aattatttag atgaatggaa agcataagaa attacattta tattaatgtt
     2701 ccaaagtagc caagtatctt tcagcatcta aagctgccat acagccactt cctgcagcag
     2761 ttactgcttg cctgtatatt ttatcttgta catctcctgc agcaaaaata cctgctaaac
     2821 ttgtttttga agaacctggt attgttttaa tataacctgt atcatccata tctatccagc
     2881 cagtaaaaat tttgctattt ggctgatggc caattgcaac aaaaaaacca cttattgcaa
     2941 tatctatggt tgaatttgtt aaggtgtttt ttattcttac accttctgtt ttgttatctc
     3001 ctaaaatttc atctgtttca ctgttccaat aaattttaat attaggtgtt gccaaaactc
     3061 tttcttgcat tactttactt gctctcatag catctcttct aacaatcatg tgtacagtgc
     3121 t
//
LOCUS       DAOQPK010000243         3119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_23204_length_3119_cov_4.689621, whole genome shotgun sequence.
ACCESSION   DAOQPK010000243 DAOQPK010000000
VERSION     DAOQPK010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23204_length_3119_cov_4.689621"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..846)
                     /locus_tag="PLU36_09605"
     CDS             complement(79..846)
                     /locus_tag="PLU36_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HMZ47046.1"
                     /translation="MSVTDKLKHIRIIRTCVYAVVGVITYPGLRIFNKLKIEGTENLK
                     NLPKKNVLFVSNHQTYFADVITFIHILSAVKWRKKNRLGLPYYLLNPFTNLYYVAAEE
                     TMKGSLISRFFILAGALTVKRTWRAEGKEVRRGLDPSDTRKINRALDSSWVITFPQGT
                     TKPFAPGRKGTGYIIKNNQPTVVPVVINGFWRAFNKKGLAFKKKGSILTVKFKEPMVI
                     DYTQPAEIILEQVMDAIEQSKKYMLKGKHHLMNVLDK"
     gene            980..1648
                     /gene="hxpB"
                     /locus_tag="PLU36_09610"
     CDS             980..1648
                     /gene="hxpB"
                     /locus_tag="PLU36_09610"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: protein motif:HMM:NF008087.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HMZ47047.1"
                     /translation="MNFSTVIFDMDGLLIDSEPLWNKAASKVFNEYGVKLSNEQYNTT
                     TGLRTREFVQWWFKKFNIGDEELLKAETKIIDLIVQMVKENKGAIMPGVNYIIDFFQK
                     KNFKIGLATSSPMQLIDVVIELTNSKNYFQAIASAENLTYGKPHPQVYLDCAQQLNVH
                     PHECICFEDSFNGMIAAKAARMTCVVVPHHTQLKDERWGAADLKLTSLQNFGELHFGL
                     LSNK"
     gene            1726..>3119
                     /locus_tag="PLU36_09615"
     CDS             1726..>3119
                     /locus_tag="PLU36_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HMZ47048.1"
                     /translation="MELSKNFEPQLVDNKWYSHWQTKGYFNSVVDATKQPYTIVIPPP
                     NVTGVLHMGHCLNNTIQDILIRHARMNGKNACWVPGTDHASIATEAKVVQMLRERGIT
                     KSSLSRDEFLKYAWEWKEKYGGIILQQLKKLGCSLDWNRTAFTMDDDYYKSVINTFIQ
                     LYKDGIIYRGLRMVNWDPASKTALSDEEVIFKETDSKLYFVKYAMASDANQFIMVATT
                     RPETILGDVAICVNPTDERYKNLIGKEVIVPIINRKIPIIADEYVDVTFGTGALKITP
                     AHDKNDNELGRKYQLKAINTLDEDGKFTIDELLEDNPSEVVKNYHGIDRFELRKIIAK
                     DISELGQLEKVEEYKSQVGTSERTGAIIESRLSMQWFMNMKMFMEKNPEALNAVLNDT
                     INFHPSKLKNTYRHWLINVKDWCISRQLWWGQRIPAWYDDEGNFVVAATKEEAIIQFK
                     TQFPSSNLQTITQE"
BASE COUNT         1143 a          437 c          515 g         1024 t
ORIGIN      
        1 cacattcatt aaaaaatata tgcttaaagg caaacatcat ttaatgaatg tgttagataa
       61 atctctggtt tttttatgtt atttatctaa cacattcatt aaatgatgtt tgcctttaag
      121 catatatttt ttgctttgtt caatagcatc cattacttgc tctaaaatta tttcagcagg
      181 ttgtgtgtaa tcaattacca ttggctcttt aaatttaact gttaatatgg atcctttctt
      241 tttaaatgct aatccttttt tattaaatgc acgccaaaag ccattaatta caactggtac
      301 aacagttggc tgattatttt taattatata accagtacct tttctgcctg gtgcaaaagg
      361 tttggtagta ccttgcggaa aggtgattac ccaacttgaa tctaaagctc tgtttatttt
      421 tctggtatca ctagggtcta atcctcttct tacttctttg ccctctgcac gccaggttct
      481 ttttacagtt aatgcaccag ctaaaataaa aaatctactt attaaactgc ctttcattgt
      541 ttcttctgct gccacataat acaaattagt gaatgggtta agtaagtaat aaggcaaacc
      601 taatctattt ttttttcgcc atttaactgc cgatagaata tgtataaaag taattacatc
      661 ggcaaaataa gtttgatgat tacttacaaa aagtacattt ttttttggta aattttttaa
      721 attttctgtt ccttctattt ttaatttatt gaatattctt aaaccaggat aggtaataac
      781 accaacaaca gcataaacgc aagtacgaat aattctaata tgtttaagtt tatctgtaac
      841 actcattaca attaatttga gtataatatt aaatgcaata tagctattaa atttttattg
      901 aacctaaagc agtaaataat ttgggtgtat ttaactatct ataaaacatt caaatacatt
      961 tgcaacaatt ataattgata tgaattttag tacagtaatt tttgatatgg atggtttatt
     1021 aattgatagt gaaccattgt ggaataaagc agctagtaaa gtttttaatg aatatggtgt
     1081 taaattaagt aatgaacaat ataatactac aacaggttta agaaccagag agtttgtaca
     1141 atggtggttt aaaaaattta atataggcga tgaagaatta ttaaaggccg aaacaaaaat
     1201 tattgattta atagtacaaa tggtcaaaga aaataaaggt gcaataatgc caggtgtaaa
     1261 ttatattata gatttttttc aaaagaaaaa ctttaaaatt gggttagcca catcatcgcc
     1321 aatgcaatta atagatgtag tgatagaact aaccaactct aaaaactatt ttcaggcaat
     1381 tgcatctgcc gaaaatttaa cctacggaaa accacaccca caagtttatt tagattgtgc
     1441 ccaacaatta aatgttcacc ctcatgagtg catttgtttt gaagattctt ttaatggaat
     1501 gatagctgca aaagctgcta gaatgacttg tgttgtggtg ccacatcata ctcaattaaa
     1561 agatgaacgt tggggagcag ccgatttaaa gttaacttct ttacaaaatt tcggagaact
     1621 tcattttggg ttattaagta ataaataata aactctttat tcttttgctt ttaacttcat
     1681 agcctttgct tatttttaca gccttttata aaataaggct aataaatgga attaagtaaa
     1741 aactttgaac ctcaacttgt agataataaa tggtatagcc attggcaaac aaaaggctat
     1801 tttaatagtg tggtagatgc caccaaacaa ccttatacca ttgttattcc accacctaat
     1861 gtaacaggtg ttttacacat ggggcattgt ttaaataaca ctattcaaga tatattaatt
     1921 aggcatgcaa gaatgaatgg taaaaatgca tgttgggtgc caggaactga tcatgcaagt
     1981 attgctactg aggcaaaagt ggtacaaatg ttaagagaac gtggtattac aaaatcatct
     2041 ttatcaagag atgaattttt aaaatatgca tgggagtgga aagaaaaata tggaggcatt
     2101 attttgcagc aattaaaaaa attaggttgc agtttagatt ggaatagaac agcttttaca
     2161 atggatgatg attattataa aagcgttatc aatacattca ttcaattata taaagatggc
     2221 attatatatc gtggcttaag aatggttaat tgggacccag caagtaaaac agctctaagc
     2281 gatgaggaag taatttttaa agaaacagac tctaaacttt attttgtaaa atacgcaatg
     2341 gcttctgatg ctaatcaatt tattatggtt gcaacaacaa gaccagaaac tattttaggc
     2401 gatgtggcta tttgtgtaaa ccctacagat gaacgttaca aaaaccttat tggcaaagaa
     2461 gtgattgtgc caattataaa cagaaaaata cccatcattg cagatgaata tgtagatgtt
     2521 acttttggaa caggggcatt aaaaataact ccagctcacg ataaaaatga taatgaatta
     2581 ggaagaaaat accaattaaa agcaatcaat acattagatg aagatggaaa atttacaata
     2641 gatgaattgt tagaagataa tccttctgag gttgttaaaa attatcatgg tattgacagg
     2701 tttgagctaa gaaaaataat tgcaaaagac atttctgaat taggccagtt agaaaaagta
     2761 gaagaatata aaagccaggt aggtacaagc gaaagaactg gagcaattat agaaagcagg
     2821 ctttctatgc agtggtttat gaacatgaaa atgtttatgg aaaaaaatcc agaggcactt
     2881 aatgcagtac taaatgatac aataaatttt catccttcta aattaaaaaa tacttacagg
     2941 cattggttga ttaatgtaaa agactggtgt atatcaagac aactttggtg gggtcaaaga
     3001 ataccagcat ggtatgatga tgagggaaat tttgtagtag ctgcaacaaa agaagaagct
     3061 attatccaat ttaaaacaca gtttccatcc tccaatttac aaactattac acaagagga
//
LOCUS       DAOQPK010000244         3102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_23423_length_3102_cov_4.408927, whole genome shotgun sequence.
ACCESSION   DAOQPK010000244 DAOQPK010000000
VERSION     DAOQPK010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23423_length_3102_cov_4.408927"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1232
                     /locus_tag="PLU36_09620"
     CDS             <1..1232
                     /locus_tag="PLU36_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HMZ47049.1"
                     /translation="MPHNLYLHSALVQTRKIGSDTASIKKAIRFNIIDSTIALNAAFL
                     VNAGILVLAASTFFVTGHQNVTKIEDAHKLLSPLLGTTVAPFLFAIALIAAGQSSTVT
                     GTLAGQIVMEGYLKLRINPWLRRLITRLIAVIPAVLVILIYGDEKVDALLVLSQVVLS
                     VHLGFAVIPLIHFVSDKKTMGEFAIKPVTKIAAWVIAIVLIFLNAQMVIGQCIEYFEN
                     GGALFWKIIILLSAALFVWLFLMMTFLPFASNRIRKTSTSIHNSSNKLTNLIVQPPTT
                     IAIALDFSEGDEKLIANALAQGNKESVFILIHIVESASAKYLGSDVDDDEARKDMDNL
                     ENYCNQLKSLGYLAIAQIGYRNRVKEIQRIVQNTNSQLLIMGAHRHSGLKDYIFGETI
                     DAVRHGLNIPVLIVNVK"
     gene            1349..1603
                     /locus_tag="PLU36_09625"
     CDS             1349..1603
                     /locus_tag="PLU36_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47050.1"
                     /translation="MYIQSMHPNTTTGNSYLHIVACKLEELTIKVLDLQGMIAKKITL
                     PVQAGNQQLKINLNDLTGGNYVLNAFNGDVFVKSIPFIKL"
     gene            1756..>3102
                     /locus_tag="PLU36_09630"
     CDS             1756..>3102
                     /locus_tag="PLU36_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HMZ47051.1"
                     /translation="MQFYKNNLTNQELRSIYKALLLPRMIEEKMLVLLRQGKISKWFS
                     GIGQEAIAVGATLALDKEEWIMPLHRNLGVFTTRQMPLKQLFMQWQGNKEGYSKGRER
                     SFHFGTKEHFICGMISHLGPQLAIADGVALSYKLKKQDKVTVAFTGDGGTSEGDFHEA
                     LNVAAVWNLPVIFIIENNGYGLSTPVNEQYKCESLITKAKGYGMEGVQIDGNNILAVY
                     DTIKGVREYCIKNQKPYLIECMTFRMRGHEEASGTKYVPSNLFEEWAKKDPVVNFENY
                     LLQENILTTDIINIIKSEIKEYIESDLQEAYKATGLIPNTQEEINDVFANYSCHSTIQ
                     KVVNEKGEWVEEVGETEDIRFIDAIKQGLQQSMQKHHNLILMGQDIAEYGGAFKITEG
                     FVQEFGKERVRNTPLCESAIIGTALGLSLEGNKSMVEMQFADFVSVGFNQIVNNLAK"
BASE COUNT         1120 a          398 c          568 g         1016 t
ORIGIN      
        1 taatgccaca taatttatac ttacatagtg ctttggtaca aacaagaaaa attggtagcg
       61 atactgctag cattaaaaaa gcaattcggt ttaatattat agatagtaca attgcactta
      121 atgcagcttt tttagttaat gcaggtattt tagttttagc agcatctacc ttttttgtaa
      181 caggccatca aaatgtaact aaaattgaag atgcacataa attattatct ccattattgg
      241 gcacaacagt agccccattt ttatttgcaa ttgcattaat tgctgcaggg caaagcagta
      301 ctgttacagg tacattagca gggcaaattg ttatggaagg ttatttgaag ttaagaatta
      361 atccatggct tagaaggtta attacaaggc ttattgcagt tattcctgct gttttggtaa
      421 ttctaattta tggcgatgaa aaagttgatg cactattggt tttaagccag gttgtattaa
      481 gtgttcattt aggttttgct gtaatacctt taatacattt tgtaagcgat aaaaaaacta
      541 tgggtgaatt tgctataaag cctgtaacca aaattgcagc atgggtaata gcaattgtac
      601 ttattttttt gaatgctcaa atggtaattg gccagtgtat tgaatatttt gaaaatggtg
      661 gagcattatt ttggaaaata ataatattac tttcagcggc tttatttgta tggctttttt
      721 taatgatgac atttttacct tttgcatcaa atcgcattcg taaaacatct actagtattc
      781 ataatagctc aaacaaatta actaatttaa tagtgcaacc tcctacaaca attgccatag
      841 ctttagattt tagtgaggga gatgaaaaac ttattgctaa tgctttagca cagggaaata
      901 aagaatctgt atttatatta attcatattg tagaaagtgc atctgcaaaa tatttaggaa
      961 gtgatgtaga tgatgatgaa gcaaggaagg atatggacaa tttagaaaac tactgtaatc
     1021 aattaaaatc tcttggctat ttagctatag cacaaattgg atacagaaat agggttaaag
     1081 aaatacaaag aattgttcaa aatactaatt ctcaactatt aataatgggt gcacacaggc
     1141 atagtggttt aaaagattac atttttggag aaactattga tgctgttagg catggtctaa
     1201 atattcctgt attaattgta aatgtaaaat aaacctaatt tttccttatt tatccctttc
     1261 aattttatta ttaaagctaa gcaacataat attataatgt tgtatctaat gcatactata
     1321 attttattta ttagataaac tatagcttat gtacattcaa agtatgcacc caaatactac
     1381 aacaggcaat agttatttgc atattgtggc ttgtaaatta gaggaactaa caattaaagt
     1441 attagacctt caaggcatga ttgctaaaaa aattacactt cctgttcaag ctggaaatca
     1501 gcagctaaaa attaatttaa acgatttaac tgggggtaat tacgttttaa atgcatttaa
     1561 tggcgatgtt tttgtaaaaa gtattccgtt tataaaacta taaaaatttg ttagggggtt
     1621 aatttgtgaa agtccttact atttatatag tagggctttt tttattctaa aaaaacgcaa
     1681 ccttttcaca tgtatcaata attaatttgc caacattgta gtacaaaaat tatcttagcg
     1741 ttttaaagaa gtaaaatgca attctataaa aataatttaa ctaatcaaga gttacgatct
     1801 atatacaagg cattgttatt gccaagaatg attgaggaaa aaatgctcgt attacttcgt
     1861 cagggtaaaa taagtaaatg gtttagtggc attgggcaag aggcaatagc tgttggtgca
     1921 actttagctt tggataaaga ggaatggata atgcctttac atagaaattt gggtgtattt
     1981 actaccaggc aaatgccctt gaaacaatta tttatgcagt ggcaaggtaa taaagaaggt
     2041 tatagcaaag gaagagaaag aagttttcac tttggtacaa aagagcattt catttgtgga
     2101 atgatctctc accttggccc acaactagcc attgctgacg gtgtggctct ttcttataaa
     2161 ttaaaaaaac aagataaagt tacagttgct tttacaggag atggtggcac tagcgaaggc
     2221 gattttcatg aagcattaaa tgtagctgcc gtttggaatt tgcctgtaat ttttattatt
     2281 gaaaataatg gctatggttt aagtacaccg gtaaatgaac aatacaaatg cgaaagtctt
     2341 attactaaag caaaaggtta tggtatggag ggtgtgcaaa ttgatggcaa taatatttta
     2401 gctgtttatg atactattaa aggagttaga gaatattgca ttaaaaatca aaagccctat
     2461 ttaatagaat gtatgacctt tagaatgagg gggcacgaag aagcaagtgg tacaaaatat
     2521 gttccttcaa atttatttga agagtgggca aaaaaagacc ctgtagtaaa ttttgaaaat
     2581 tatttattac aagaaaatat tttaactacc gatattataa atattattaa atcagaaatt
     2641 aaagagtaca ttgaatcaga cctacaagaa gcttataaag caactggttt aataccaaat
     2701 acccaagaag aaataaacga tgtttttgca aattattctt gccattcaac aattcaaaaa
     2761 gtagtaaatg aaaaaggaga atgggttgaa gaagttggag aaacagaaga tataagattt
     2821 atagatgcca taaaacaagg tttgcaacaa agtatgcaaa aacaccacaa ccttatttta
     2881 atggggcaag atattgccga atatggcggt gcttttaaga ttaccgaagg ctttgtgcaa
     2941 gagtttggaa aagaaagagt acgcaataca ccactttgtg aaagtgctat aattggtact
     3001 gcattgggtt taagcttaga gggaaataag agtatggtag aaatgcaatt tgccgatttt
     3061 gtaagtgtag gctttaatca aattgtaaat aatttagcta aa
//
LOCUS       DAOQPK010000245         3099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_23466_length_3099_cov_4.499014, whole genome shotgun sequence.
ACCESSION   DAOQPK010000245 DAOQPK010000000
VERSION     DAOQPK010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23466_length_3099_cov_4.499014"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PLU36_09635"
     CDS             complement(<1..282)
                     /locus_tag="PLU36_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HMZ47052.1"
                     /translation="MTDTIINLDSINPIEFFGVNNGKFDLLKKKFPLLKILSRGTHIK
                     LSGAPEQIDSAKEKINLIVQYLERNGHLSENYFEQILGGDDAETIDNFVD"
     gene            complement(457..1467)
                     /locus_tag="PLU36_09640"
     CDS             complement(457..1467)
                     /locus_tag="PLU36_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HMZ47053.1"
                     /translation="MKKLIIVLLIFILGFSANAQDPHFSQFFSSPLTLNPAFTGKFNG
                     NYRIAGNYRNQWPTINNAFITSTASVDFQLMQKKLVYTDNWGVGIMGYTDNSANGAVK
                     FNYASLSTAYHKGLDEDGYNQIGVGFQITYANMLINTTSLKFEDQLTTSGFTGVTSEI
                     FNNPSSLKSNFIDLNAGVLYTGSSSDKNSYYAGVSIYHVTRPKQTFTGANYLLNMRTT
                     IHGGGNFDMNDNTTLHVSALQSFQGGAAETVVGGAIQFNVSGNEAPKPTSFYAGAWLR
                     FKDALIPYIGLEFDDFRLGATYDINTSSLKNASQKRGGLEISLIYIRRPNTDRNIRCP
                     KF"
     gene            complement(1550..2227)
                     /gene="upp"
                     /locus_tag="PLU36_09645"
     CDS             complement(1550..2227)
                     /gene="upp"
                     /locus_tag="PLU36_09645"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HMZ47054.1"
                     /translation="MKRFKKLNMVINLSSKNSLVNNWISELRDINVQNDRLRFRRNLE
                     RFGEVAAYEISKNFNFKTVEIQTPLGTHNSNVIAEQPVLATILRAGLPLHNGMLNYFD
                     KADNAFISAYRKHHRDGSFEISLEYVSCPSLDNRTIIISDPMLATGASLVKTIAILQD
                     RWQINKIYVVCAIASTVGIEYVERECGNNIEIWCGDIDEELTAKGYIVPGLGDAGDLA
                     FGTKAQS"
     gene            complement(2237..2851)
                     /locus_tag="PLU36_09650"
     CDS             complement(2237..2851)
                     /locus_tag="PLU36_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HMZ47055.1"
                     /translation="MLTQAFYTNKNTIDVAKNLLGKILVTKMEGLVTKARIVETEAYC
                     GITDKACHAYNNKRTKRTEIMFANGGVCYIYLCYGMHYLFNVVTNTINNPHAVLVRAV
                     EPLEGLEIMQKRTGKKENDFTITKGPGNVTKALGITNNQNGYSLLQNTIYIEEDDFIL
                     SSKQIINTPRIGVHYAKEDALLLYRFFIKNNLFVSGKKIDNFAN"
     gene            2911..>3099
                     /locus_tag="PLU36_09655"
     CDS             2911..>3099
                     /locus_tag="PLU36_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47056.1"
                     /translation="MLKDFIIALQAYRKAHLFIQKYKLWKWIILPGLFYCILFLVGMY
                     FFGNTANSFIEWLKGFLIN"
BASE COUNT         1001 a          496 c          455 g         1147 t
ORIGIN      
        1 gtccacaaaa ttgtcaatag tttccgcatc atcgccacct aatatttgct caaaatagtt
       61 ttcacttaaa tgtccatttc tttctaaata ctgaacaatt aaatttattt tttcttttgc
      121 agagtctatt tgttctgggg caccacttag tttaatatgt gttcctctag atagaatttt
      181 aagtaatgga aatttttttt tcagtaaatc aaacttgcca ttgtttacac caaaaaattc
      241 aataggatta atggaatcta gattaatgat tgtatctgtc aaaataataa tggttttaaa
      301 tagattaata atttgagttg agttactact aacctttcct acccaaatat aattttaata
      361 attgtagata gtattaatta ttattcacaa actgtggata ggttttacaa cagtttaagc
      421 aataagaagg aagcattttt tgattaaaat ataatactaa aatttggggc atctaatatt
      481 tctatctgta ttaggccttc taatataaat taatgagatt tctaaaccgc ctcttttttg
      541 gcttgcattt tttaaggaag aagtattaat atcataggtt gcacctaatc taaaatcatc
      601 aaactctaat cctatatatg gtattaaggc atctttaaaa cgtagccatg caccagcata
      661 aaagctagta ggttttggtg cttcgttgcc acttacatta aattgtattg caccaccaac
      721 aacagtttcg gctgcaccac cttgaaaact ttgtaatgca ctaacatgta aagtagtatt
      781 atcattcatg tcaaagtttc ctccaccatg tatggtagta cgcatattta ataaatagtt
      841 agcacctgta aaagtttgct ttggtcttgt aacatggtat atacttacgc cagcataata
      901 actgttttta tcgctactgc tacctgtata taaaacccct gcatttaggt ctataaaatt
      961 tgattttagt gaagaagggt tattaaaaat ttcggaagta acaccagtaa aaccagaggt
     1021 ggtaagttgg tcttcaaatt ttaagcttgt agtatttata agcatattgg cataggtaat
     1081 ttgaaagcca acacctattt gattgtagcc atcttcatct aatcctttat gataggcagt
     1141 agataaagaa gcataattaa atttaactgc accatttgca ctattatctg tatagcccat
     1201 tataccaact ccccaattat ctgtataaac taattttttt tgcattaact gaaagtcaac
     1261 agatgctgta cttgtaataa atgcattatt tatagttggc cattggtttc tgtaattacc
     1321 tgcaatgcgg taattgccat taaacttacc tgtaaatgca gggttaagtg taagtggcga
     1381 tgaaaaaaat tgagaaaagt gaggatcctg agcatttgca ctgaaaccca aaataaaaat
     1441 caataaaaca ataattaact tcttcatgaa aattaaaaga tgccgaaagt aagtgaaatg
     1501 ttgtaaatga ataaaattac ttgatgaata ttttagtaat aggtttttat taagattgag
     1561 ctttggtgcc aaatgctaaa tcgccagcat cgcctaagcc tggtacaatg tagccttttg
     1621 cagttaattc ctcatcaata tcgccacacc aaatttcaat attattacca cattcacgtt
     1681 caacatattc aatacctaca gtactggcaa ttgcacatac aacatatatt ttatttattt
     1741 gccatctatc ttgtaaaata gcaattgttt taaccaaaga tgctccagtt gcaagcattg
     1801 ggtcgctaat aataattgtt ctgttatcta aagaagggca gcttacatat tctaagctta
     1861 tttcaaaact gccatcacgg tggtgttttc tataagcaga aataaaagca ttatctgctt
     1921 tatcaaaata attaagcatg ccattgtgta aaggtaaacc tgctcttaaa atagttgcta
     1981 aaacgggttg ttctgcaatt acattactat tatgtgtgcc taatggcgtt tgaatctcta
     2041 ctgttttaaa attaaaattt ttacttattt cgtaggctgc cacttcgcca aatctttcaa
     2101 ggtttctacg aaaacgcaac ctgtcgtttt gtacattaat atctcttaat tcgctaatcc
     2161 agttgtttac taaactgttt ttactgctta agtttattac catatttaat tttttaaaac
     2221 gcttcaaagc taattgttaa ttggcaaaat tgtcaatttt ctttccgctc acaaataaat
     2281 tgtttttaat gaaaaatcgg tataataata gtgcatcttc tttagcataa tgtactccta
     2341 ttcttggggt attaataatt tgttttgaag acagtataaa atcatcttcc tctatataaa
     2401 ttgtattttg caaaaggcta tatccatttt gattgtttgt tatgcctaaa gccttggtta
     2461 cattgcctgg acctttagtt attgtaaaat cgttttcctt ttttcctgtt cttttttgca
     2521 ttatttctaa gccttctaat ggttcaacag ctctaaccaa aactgcatgt gggttattaa
     2581 ttgtattagt tactacatta aataaataat gcattccata gcataaatat atataacata
     2641 caccaccgtt agcaaacata atctccgtac gttttgttct tttattattg tatgcatgac
     2701 aggctttgtc tgtaatgcca cagtatgctt ctgtttctac aattctggct ttggtaacta
     2761 agccttccat tttggtaact agtattttac ctaacaaatt ttttgcaaca tctattgtgt
     2821 ttttattggt ataaaatgct tgggttagca taaaatattc tatttattgt taattgcttt
     2881 ggtatcttag caacggaaat attaagatta atgttaaaag actttattat agcattacaa
     2941 gcctatagaa aggcacattt gtttatacaa aaatataaac tatggaaatg gataatttta
     3001 ccagggctat tttattgcat tttgttttta gtaggcatgt atttttttgg taatactgct
     3061 aatagtttta ttgaatggct gaaaggcttc ttaataaat
//
LOCUS       DAOQPK010000246         3090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_23596_length_3090_cov_4.779242, whole genome shotgun sequence.
ACCESSION   DAOQPK010000246 DAOQPK010000000
VERSION     DAOQPK010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23596_length_3090_cov_4.779242"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="PLU36_09660"
     CDS             complement(<1..495)
                     /locus_tag="PLU36_09660"
                     /inference="COORDINATES: protein motif:HMM:NF024886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HMZ47057.1"
                     /translation="MQVTQQNSTIVDFLNYIKFEKRYSLHTVIAYQTDLEQFFVYINA
                     QYDSPTVQSINSMMIRSWLACLKGDEKLSAKSLNRKISSLKTFFRHLLKNQVITQTPM
                     STVVAPKISKRLPVFVQENNLETLFNHVEFTNNWKGKTEQLVLQIFYHTGIRLSELIQ
                     LKTVN"
     gene            complement(560..757)
                     /gene="rpsU"
                     /locus_tag="PLU36_09665"
     CDS             complement(560..757)
                     /gene="rpsU"
                     /locus_tag="PLU36_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HMZ47058.1"
                     /translation="MLIIDSKDCENIDKALKKYKKKFEKSKTLLQLRERQTFTKPSVK
                     RRGEVLKAIYKQQIASGKIEG"
     gene            1001..2935
                     /locus_tag="PLU36_09670"
     CDS             1001..2935
                     /locus_tag="PLU36_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47059.1"
                     /translation="MSIKNDSCVLSYPIFYFFSNQLNNDIIPFWHFNMHFGFALYADP
                     GTPFLNPIFWLFALANSSIYTYVLYILFHIVLGSIGMFLLGKQLGFEQKTNTILAVAY
                     IAGGYFVAHLQHSNHIIECTYLPFVINFLYALFKKPNLKNTLLLGLFLYLFTISGYPG
                     FAIGMPYYIIILGIGYIINQSYKKKFPEKLRAMGLFCISVAIAIILCLPFLYALYTNM
                     ENFQRASTFSGNSYLFEGGASPVLGFASFILPLASVVKNSPFLSSDMAWNNMYIGIVV
                     LIFFLASIKYKNLFIILPHLMAIAFFLDISFEGQIKQLFFKLPLLNFLRYNGGLRIYA
                     MLSMIIVAGYTINNFFITEKLDTKRLVTIIKILFIIVSAVLVTTIIYSLINHSFIFNN
                     EGSLAKKILHINLSQAIILQSVYVLIVLFFFKKWINNKRSVLLIALVDIIICFWVNLP
                     FSGLSIKSMPTITRDIEASSKFINEQQSFTIQTDSVLKPAINHVVYAPALLSNKVGVI
                     PPHAYPSGKKTYFEFIEKNGLNYFNNKPTCFLQSKNTVALPYTINAESIKIECSTTQQ
                     DTLIIFQNFDKNWKAYSNNNMLTITQWKNNFMQIILPANTKNIQLIYNDNAINKLLFV
                     PIIGLLTILFILLYTYKKNN"
BASE COUNT         1028 a          434 c          414 g         1214 t
ORIGIN      
        1 atttacagtt ttaagttgta ttaattcact tagcctaata cctgtatgat aaaatatttg
       61 aagtactaat tgttctgttt tacctttcca gttattggta aactctacat gattaaataa
      121 ggtttctaaa ttattttctt gaacaaaaac cggtagtctt ttactaattt ttggagctac
      181 tactgtggac attggcgttt gcgtaattac ctgatttttt agtaaatgcc tgaagaaagt
      241 ttttaaggaa gaaatttttc tgtttaatga ttttgccgat aacttttcat cgccttttaa
      301 acaagccagc caacttctta tcatcatgga attaatagat tgtacagttg gactatcgta
      361 ttgggcattt atatatacaa aaaactgttc caaatcggtt tggtaggcta tcactgtatg
      421 caatgaatag cgtttctcaa attttatata attaagaaaa tctacaatag tgctattctg
      481 ttgagttact tgcataaaaa aagtagctca agttagtgta actaaagcta cttaaatatg
      541 tttgggtata aatgttttac tacccttcta tttttccact agctatttgt tgcttgtaga
      601 ttgcttttaa aacctcgcct ctgcgtttta cagaaggttt agtaaaagtt tgacgttctc
      661 ttaattgtaa caaagtttta cttttttcaa actttttctt gtactttttt agagctttgt
      721 ctatgttttc gcaatcttta gaatcaataa ttagcatatt tatatacctc ctttaatgat
      781 gaaatggggt gcaaatatac tagcttagtg cataatacca aatattttat aaatgtatta
      841 tagcaattag tatagtttta cagctattaa aagcattttt aaataagaag tatttataaa
      901 aatgttatta caaactatat taaaatatat ttataaaaaa agttggctta ttgttttaat
      961 aggaataagt attgcttatt ttcctttaat aagctctata atgtctatta aaaacgatag
     1021 ctgtgtattg tcttatccta tattttactt tttcagtaac cagttaaata acgacattat
     1081 tcctttttgg cattttaata tgcattttgg ctttgccctt tatgccgatc ctggaactcc
     1141 tttcttaaat ccaatatttt ggttgtttgc gttggcaaac agttctattt acacctatgt
     1201 actatatatt ttatttcata ttgtattggg ttcaattggt atgtttttac taggcaaaca
     1261 attaggtttt gagcaaaaaa caaatacaat tcttgcagtt gcttatattg ctggtggtta
     1321 ttttgttgca cacttgcaac actccaacca tattattgaa tgtacctact tgccttttgt
     1381 aattaatttc ttatatgctt tatttaaaaa gcccaattta aaaaacactt tattattggg
     1441 tttattcttg tatttgttca ccattagtgg ttaccctggt tttgcaattg gtatgcctta
     1501 ctatattatt atacttggaa ttggctatat aattaatcaa tcttataaaa aaaaatttcc
     1561 agaaaagctt cgagcaatgg ggttgttttg tataagtgtt gcaattgcaa ttattttatg
     1621 cctgcctttt ttatatgcac tctatacgaa tatggaaaat tttcaaaggg catctacttt
     1681 tagtggtaat agttatttgt ttgaaggtgg ggcaagccct gttttaggtt ttgcaagttt
     1741 tattttacca ttagcaagtg ttgtcaaaaa tagccctttc ttatcgtctg atatggcttg
     1801 gaataatatg tatattggta ttgtagtttt aatattcttt ttagcatcta ttaaatacaa
     1861 aaacttattt attattcttc ctcatttaat ggctattgct ttctttttag atatttcatt
     1921 tgaaggacaa ataaaacagc tattctttaa acttccatta ttaaattttt tacgctacaa
     1981 tggtgggtta agaatttatg ctatgcttag catgataatt gttgcaggat atactattaa
     2041 taattttttt attactgaaa aattagatac taaaaggtta gttaccatca ttaaaatatt
     2101 atttataata gttagtgctg ttttagttac cacaataatt tacagtttaa ttaatcattc
     2161 ttttattttt aataatgaag gaagtttagc aaaaaaaatt ttacacatca atctttctca
     2221 ggcaattatt ttacaaagtg tttatgtatt aattgtatta ttttttttta aaaaatggat
     2281 caataacaaa agaagtgtat tgcttattgc tttagtagat ataattattt gcttttgggt
     2341 aaacctaccc ttttctggtt taagcattaa aagtatgcca accataacaa gagatataga
     2401 agcttcttca aaatttatta atgagcaaca aagttttact attcaaaccg attctgtttt
     2461 aaaacctgct ataaaccatg ttgtttatgc acctgcatta ttaagtaata aagttggggt
     2521 aattccgcct catgcttatc catctggtaa aaaaacatat tttgagttta tagaaaaaaa
     2581 tggcttaaac tatttcaata ataaacctac ttgtttttta caatctaaaa atacagttgc
     2641 tttaccttac actattaatg cagagagtat aaaaattgaa tgtagcacca cccaacaaga
     2701 tactttaata atttttcaaa actttgataa aaactggaaa gcatacagca ataataacat
     2761 gctaaccata acacaatgga aaaacaattt catgcaaatt attctgcctg caaacaccaa
     2821 aaacattcaa ttaatttata acgataatgc tataaacaaa ttactttttg tgcctataat
     2881 tggcttatta actatactat ttattttact ttatacttat aaaaaaaata attaactaaa
     2941 acaaattgca aactaatgtt taccggaatt attgaaacca ctggtgtaat taaacaaatt
     3001 attaaacaaa atagcaacct cacatttgtt attgaaacca aatttactaa cgaattaaaa
     3061 gttgatcaaa gcattgcaca caatggtatt
//
LOCUS       DAOQPK010000247         3062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_23992_length_3062_cov_3.357832, whole genome shotgun sequence.
ACCESSION   DAOQPK010000247 DAOQPK010000000
VERSION     DAOQPK010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23992_length_3062_cov_3.357832"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="PLU36_09675"
     CDS             <1..565
                     /locus_tag="PLU36_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47060.1"
                     /translation="AIASLLTYLSIELNFKLSFSPFLLTIIAIAFQIYISIKAAKESK
                     VTKFEDAAINAVWLAFGISIMGLVLYLNIIPNSSIKLATQEGWQFTKHYLDANKPDEP
                     LTPFAPSSYSLFILLYALPTFTTGIVKNYKPMIVGAILTYVLFIISCYTSTKYDMLAG
                     TIAAISCWLIPGLILRRNYLQQKNSNV"
     gene            558..851
                     /locus_tag="PLU36_09680"
     CDS             558..851
                     /locus_tag="PLU36_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HMZ47061.1"
                     /translation="MFKDLDPILHSQLRLAIISLLIGVKEADFNFIKEKTKSTAGNLS
                     VQIQKLKDAGYIEVTKQFKDNYPLTTCSITKQGVHAFEIYVKALQGYLHTDKN"
     gene            894..1415
                     /gene="idi"
                     /locus_tag="PLU36_09685"
     CDS             894..1415
                     /gene="idi"
                     /locus_tag="PLU36_09685"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HMZ47062.1"
                     /translation="MEKVILVDEYDNEIGIEEKIKTHQAGLLHRAFSVFIVNKNGDLL
                     LQQRSLNKYHSAGLWTNTCCSHPRPNENVTNAAERRLQEEMGFTTKLKPIFQFIYNVA
                     FENGLSEHEYDHVLLGEYNGEISINEQEVADFKFVALDAIEDLIATTPEQFTTWFRIA
                     FPRLKQWLQANSH"
     gene            complement(1417..>3062)
                     /gene="rnr"
                     /locus_tag="PLU36_09690"
     CDS             complement(1417..>3062)
                     /gene="rnr"
                     /locus_tag="PLU36_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HMZ47063.1"
                     /translation="TATNNEKVIVRLVKWEKGDKKPVGEVMSILTAADENDAAMKEII
                     AAAGFALSFTDEVITETAQLHETLSEKEIAQRKDFREVLTFTIDPVDAKDFDDAISVK
                     KINDELYEIGVHIADVSYYVKPDTALDEEAYKRATSVYLPDRVNPMLPEKISNELCSL
                     RPNEDKFTFSAVFEISTNGNVKNYWLGKTVIHSNHRFTYEDVQDIINGKIGEYESEIL
                     LLHKIAQIIRTNRFKKGAINFSSQEVRFKLDENAKPISIIVKESKASHQLIEEFMLLA
                     NKTVAEHIDKSSNNNPIPFPYRVHDQPDEEKLLPFIEFAKKFGHTFNISSPEKIAESF
                     NTLLENVKGKPEQQVLEQLGIRTMAKAVYTTKNIGHYGLAIPHYCHFTSPIRRYPDVL
                     VHRILYSFLQGKTIMDKKMEEKCRHCSEREKAAMDCERAGNSYKQVEFLKEHLGEDFE
                     GVISGVASFGFWVETLAHKCEGMVSIVSLSDYDDFKFVETDFCLVGKRSGKTFRIGDK
                     VTIKVVAANLEKRKLDYEWVTNDNMEATKPVKKKIKKQK"
BASE COUNT          998 a          509 c          436 g         1119 t
ORIGIN      
        1 agcaattgct tctttattaa cttatttaag tattgagtta aattttaaat taagttttag
       61 cccgtttctt ttaacaatta ttgccattgc atttcaaatt tatatttcaa taaaagcagc
      121 caaagagagt aaggtaacaa agtttgaaga tgctgcaatt aatgctgttt ggttagcatt
      181 tggaatttct attatgggtt tagtattata cttaaatatt attcctaact caagtataaa
      241 gcttgctacg caagaaggtt ggcagtttac taaacattat ttagatgcca ataaacccga
      301 tgaaccatta accccatttg caccaagcag ctattctctt tttattttat tatatgcttt
      361 accaactttt acaactggta ttgtaaaaaa ttataaaccc atgatagtgg gtgcaatttt
      421 aacctatgtt ttgtttatta tatcttgtta taccagcaca aaatacgata tgttagcagg
      481 tacaatagca gctatttctt gttggttaat tcctggttta attttaagaa gaaattattt
      541 gcaacaaaaa aacagcaatg tttaaagacc tagatcctat attacactct caactaaggc
      601 ttgcaataat tagtttactt attggggtaa aggaagctga ttttaatttt ataaaagaaa
      661 aaacaaaatc tactgcaggc aatttatctg ttcaaataca aaaattaaaa gatgctggtt
      721 atattgaagt aaccaaacaa tttaaagata attatccctt aactacatgt tcaattacta
      781 aacaaggagt acatgcattt gaaatatatg taaaagcttt acaaggatac ttacataccg
      841 ataaaaatta aaacagttga taaactttat tactcaatcc ttatttttat agcatggaaa
      901 aggtaatatt agtagatgaa tatgataatg aaattggtat tgaagaaaaa ataaaaacgc
      961 accaagctgg cttattgcat agagcattta gtgtttttat tgtaaataaa aatggtgacc
     1021 ttttattaca acaaaggtct ttaaataaat accatagtgc aggcttatgg accaatacat
     1081 gttgcagtca tccaagacct aatgaaaatg taacaaatgc agcagaaagg agactacaag
     1141 aagaaatggg ttttacaacc aaattaaaac caatctttca atttatttat aatgtggctt
     1201 ttgaaaatgg tttatccgaa catgaatacg accatgtttt gttaggagaa tacaatggag
     1261 aaattagtat taacgaacaa gaagtagcag atttcaaatt tgtagcttta gatgccattg
     1321 aagatttaat tgctacaaca cctgagcaat ttacaacttg gtttagaatt gcatttccta
     1381 ggttaaaaca atggttacaa gcaaatagcc attaatttac ttttgctttt tgattttctt
     1441 ttttacaggt tttgtagctt ccatattatc attggttacc cattcataat ctaattttct
     1501 tttttctaaa ttagctgcaa caactttaat ggttacttta tcacctattc taaaagtttt
     1561 accacttctt ttgcccacta aacaaaaatc agtttctaca aatttaaaat cgtcataatc
     1621 gctaaggctt acaatactta ccattccttc gcatttatgg gctaaagttt ctacccaaaa
     1681 tccaaaactt gccactccac taattacacc ttcaaaatct tcgcccaaat gttcttttaa
     1741 aaactccacc tgcttataac tatttcctgc acgttcgcaa tccatggcag ccttttctct
     1801 ttcgctacaa tgcctgcatt tctcttccat ttttttatcc ataatagttt taccttgtaa
     1861 aaaactatat agaattctat gtactaaaac atctggatat cttctaatag gagaagtaaa
     1921 atgacaatag tgaggaatgg ctaaaccata atggccaatg ttttttgttg tataaactgc
     1981 ttttgccatt gttcttattc ctaattgttc taacacttgt tgctctggct tgccttttac
     2041 attttctaaa agagtattaa agctttctgc aattttctcg ggtgatgaaa tattaaatgt
     2101 atgaccgaat tttttggcaa attctataaa gggtaaaagc ttttcttcgt ctggttggtc
     2161 atgtactcta taaggaaaag gtattgggtt attgttactg gatttatcaa tatgttctgc
     2221 aacagtttta ttggcaagta acataaactc ttcaattaac tgatgcgatg ccttgctttc
     2281 ttttacaata atacttattg gtttagcatt ttcgtctaac ttaaatctta cttcctgcga
     2341 tgaaaaattt atagcacctt tcttgaacct attggttcta ataatttgtg ctattttatg
     2401 cagtaataat atttcagatt catactcacc aattttgcca tttataatat cttgtacatc
     2461 ttcataagta aatctgtggt ttgaatgaat aaccgttttg cccaaccaat aattttttac
     2521 atttccgttg gtagatattt caaacacagc agaaaaggta aacttatctt cgttggggcg
     2581 taaagaacat agttcgttgg atattttttc gggcaacata gggtttactc tatctggcaa
     2641 ataaacagat gtggctcttt tgtatgcttc ttcatctaaa gctgtatctg gctttacata
     2701 ataactaaca tcggcaatat gtacacctat ttcatacaat tcgtcattta ttttttttac
     2761 agaaatagca tcgtcaaaat cttttgcatc tactgggtca atagtaaaag ttaatacttc
     2821 tctaaaatct tttctttgtg caatttcttt ttcagaaagg gtttcatgca attgagcggt
     2881 ttctgttata acttcatcag taaaagataa tgcaaaacct gcagcagcaa taatttcttt
     2941 catagcagca tcgttttcat ctgctgctgt taaaatactc atcacttctc caacaggttt
     3001 tttatctcct ttttcccatt ttactaagcg tacaattact ttttcgttat tggttgctgt
     3061 ta
//
LOCUS       DAOQPK010000248         3045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_24214_length_3045_cov_5.457525, whole genome shotgun sequence.
ACCESSION   DAOQPK010000248 DAOQPK010000000
VERSION     DAOQPK010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24214_length_3045_cov_5.457525"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1587
                     /locus_tag="PLU36_09695"
     CDS             <1..1587
                     /locus_tag="PLU36_09695"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HMZ47064.1"
                     /translation="TFYNQQKYDSAIVYFKQAVELVDESAKNLFGGSEAKKIYSEDYR
                     KVDLYNKLVAALVKTGKKEDALFYADKSNNQAVKEQMEKVGIVTDDKEKAEALQKGNE
                     LLQKKNAVEKAIAKEKAKPEKEQNKQLIASLESVKSVAESDYTNFIEDLQRKYQDLQA
                     YFSNTNPKDFNRYIDDIPDSTIVVLYIINGNQLFIFTVTNKETGIKTIDLKQDLNKQA
                     TKFLSILRNPNNATGTGAVTLRSTLKKVEDVRGDFKSEATELYNLLITPIAEQLKDMK
                     NICFIPNGKLSNIPFQCLGYNDEKKKFHFLVEDYAIFYTSKIDVFRNRFKKRNLESSL
                     ALFGNPDKTLPGATTEANAIARLVPGATVYIEEQATEDKAKDALAKFSYVHFATHGVL
                     DYEKPENSYLLFAPGLGDNNEGKLTIAEINGVKKPTSSLVVLSACETAVSKEEVKGFY
                     ISPANAFLTNRVDAVIGSLWKVPDETTNLLLQEFYTNIEKKGMTKVEALRHAQAAVSE
                     NPKYSHPFFWSAFVLYGEWR"
     gene            1640..2977
                     /locus_tag="PLU36_09700"
     CDS             1640..2977
                     /locus_tag="PLU36_09700"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HMZ47065.1"
                     /translation="MQSKQQLGFFSLTMIVISFVIGMGIFKTSSTVAAKAGTETIFYA
                     AWLIGGFIAICGALTYAEIGVRLPVTGGYYKIFAYAYHPSIGFTINILVLISNAASLA
                     VVALIGADYVSDLLFAKPSGTLFNIGIAALAIFLFYGVNLLGLQTSSKTQNVLTILKV
                     ALVVLLISSFFSNTQVEPHGYTNDTIYTYNGNNGALLLILSLVAVSFTYGGYQQTINF
                     GEEVKNTRTMQRSIITGMFIVVLLYFAINYVYVHVIGFDKMKNATAIGALLTKAWFGN
                     IGAKIFDACMFLSVLAYVNVVLLSNPRVMYAMSNDGVLPKIFSNKNNKTGVLIPGLTV
                     FALTALIITFFGKGVDDVLSFSIFLDCFGMSLSAATLLILRKRKQGEENVKGFLKNIT
                     PVLCILFVIAYSIVAVAVVIDKPMAALTGVILLAVFMLVYFLFCHKKQTVKLP"
BASE COUNT         1098 a          419 c          529 g          999 t
ORIGIN      
        1 actttttaca accaacaaaa atacgatagt gctattgttt attttaagca agcagttgag
       61 ttagttgatg aatctgctaa aaacttattt ggcggatccg aagctaaaaa aatatatagt
      121 gaagactaca gaaaagtgga tttatacaac aaacttgttg ctgcactggt taaaacagga
      181 aaaaaagaag atgccttatt ttatgcagat aaaagcaata accaagcagt aaaagaacaa
      241 atggaaaaag taggtattgt tactgatgat aaagaaaaag cagaagctct acaaaaagga
      301 aatgagttat tacaaaagaa aaatgctgta gaaaaagcta tagcaaaaga aaaagcaaag
      361 ccagaaaaag aacaaaacaa acaacttatt gcatcgttag aaagtgtaaa aagtgtagcc
      421 gaaagtgatt acaccaattt tatagaagac ctgcaaagaa aataccaaga tttacaagct
      481 tacttttcta ataccaatcc taaagatttt aatagatata ttgatgatat accagatagt
      541 accattgtag ttttatacat cataaacggc aatcaattat ttatttttac agttaccaat
      601 aaagaaacag gaataaaaac aattgattta aaacaagact taaacaaaca ggcaactaag
      661 tttttaagca ttttaagaaa cccaaacaat gccactggta ctggtgctgt aaccttaaga
      721 agtacactaa aaaaagtaga agatgttaga ggagacttta aatcggaagc tacagaactt
      781 tacaacttac taataacccc tattgcagaa cagttaaaag acatgaaaaa tatttgcttt
      841 ataccaaatg gtaaactaag caatatccct tttcaatgtt taggttataa cgacgaaaaa
      901 aagaaatttc attttttagt agaagattat gccatttttt atactagtaa aattgatgtt
      961 tttagaaata ggtttaagaa aagaaacctt gaatcttcat tagcattatt tggcaacccc
     1021 gataaaactt tacctggtgc aactacagaa gctaatgcaa ttgccagatt ggtgcctggt
     1081 gcaacagttt atatagaaga acaagcaaca gaagacaaag ccaaagatgc tttagccaaa
     1141 tttagttatg tgcattttgc tacacatggt gtattagatt atgaaaaacc agaaaactcc
     1201 tatttactat ttgctcctgg attaggagat aataatgaag gaaaattaac cattgcagaa
     1261 attaatggtg ttaaaaagcc aacaagttca ttagtggtat tatctgcctg cgaaactgca
     1321 gttagtaaag aagaagttaa aggcttttac atttcgccag ctaatgcatt tttaaccaat
     1381 agagtagatg ctgttattgg ctctttatgg aaagtgcctg atgaaacaac caatttatta
     1441 cttcaagaat tttatactaa tattgaaaaa aagggtatga caaaagtaga agcattacgc
     1501 catgctcagg cagcagttag cgaaaatcct aaatatagtc atccgttttt ttggagtgcc
     1561 tttgttttat acggcgaatg gagataaaat aaattagcta taataaaatt gcatagctta
     1621 ttttcattca aattatttta tgcaatcaaa acagcaactt ggtttttttt cactaaccat
     1681 gatagtgata agctttgtga taggtatggg tatttttaaa acatcctcta ccgttgcagc
     1741 aaaagcaggt accgaaacta ttttttatgc agcatggtta atagggggtt ttattgcaat
     1801 ttgtggggct ttaacgtatg cagaaattgg cgttaggcta cctgttacag gtgggtatta
     1861 taaaattttt gcttatgctt atcatccttc tattggtttt acaattaaca ttttagtgct
     1921 tataagcaat gcagcatctt tggctgtagt ggcactaatt ggtgccgatt atgtaagtga
     1981 tttattgttt gcaaaacctt ctggaacttt atttaatatt ggtattgcag ctttagctat
     2041 ttttttattt tatggtgtaa atttattggg cttacaaaca agtagtaaaa cacaaaatgt
     2101 gttaacaatt ttaaaagttg cattagttgt attacttatt tcatctttct tttctaatac
     2161 acaggtagaa ccacatggtt atactaacga tacaatatat acatacaatg gcaacaatgg
     2221 tgcactattg cttatactta gcttagtggc tgtttccttt acttatggtg gctaccaaca
     2281 aacaataaac tttggagagg aagtgaagaa tactcgtacc atgcaacgta gcattattac
     2341 aggaatgttt attgtagtgc tattgtattt tgctattaat tatgtttatg tgcatgtaat
     2401 tggtttcgat aaaatgaaaa atgctacagc tattggtgca ttattaacta aagcatggtt
     2461 tggaaatatt ggtgctaaaa tttttgatgc ctgtatgttt ttaagtgtat tggcttatgt
     2521 aaatgtagtt ttattaagta acccaagggt aatgtatgca atgagtaacg atggtgtttt
     2581 gcctaaaata ttttcaaata aaaataataa aacaggtgta ttaatacctg gcttaactgt
     2641 atttgcttta actgcactaa tcattacttt ttttggaaaa ggtgtagatg atgtattaag
     2701 ctttagtatt tttttagatt gttttggtat gagtttatct gcagctactt tacttatttt
     2761 aagaaaaaga aaacaaggcg aagagaatgt aaaaggattt ttgaaaaaca tcactcctgt
     2821 tttatgcatc ctttttgtaa ttgcgtatag catagttgca gttgcagttg taattgataa
     2881 gccaatggct gctttaactg gtgtaatttt attagctgta tttatgctag tttatttctt
     2941 gttctgccat aaaaaacaaa cagtaaaact tccttaacag cataaaaaac aaaaccccga
     3001 aaattcgggg ttttgaatat tagtgaaaat ataattattg tttta
//
LOCUS       DAOQPK010000249         3033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_24379_length_3033_cov_6.127602, whole genome shotgun sequence.
ACCESSION   DAOQPK010000249 DAOQPK010000000
VERSION     DAOQPK010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24379_length_3033_cov_6.127602"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLU36_09705"
     CDS             complement(<1..242)
                     /locus_tag="PLU36_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HMZ47066.1"
                     /translation="MSNNSFKPTLGLTDATMIVAGSMIGSGIFIVSADIVRNTGSAGW
                     LILVWLLTGFMTLIAALSYGELSAMFPKAGGQYVYL"
     gene            complement(333..932)
                     /locus_tag="PLU36_09710"
     CDS             complement(333..932)
                     /locus_tag="PLU36_09710"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HMZ47067.1"
                     /translation="MKNFTTVLISVLTIAVGVLFFLHFSSKKTPSLNNSNTNAVVANA
                     SGFKIAYFEMDSIENHYEYLKDVRNSLRSLEQRKSGELASLRNAGKSKIMEYQKKGNT
                     MTEQEIAMANEEIMRLENELKAQEQIKSQELQDESIRKIQDVKKTIEDFLKEYNKGKN
                     FSYILSSSSDIIYLKDTAYNITSDLIEGLNKNYKEKKKP"
     gene            complement(1069..1491)
                     /gene="aroQ"
                     /locus_tag="PLU36_09715"
     CDS             complement(1069..1491)
                     /gene="aroQ"
                     /locus_tag="PLU36_09715"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HMZ47068.1"
                     /translation="MKIAIINGPNLNLLGSRETKIYGTQTFENFIEQLEKQYPNIDFH
                     YYQSNVEGELINELQQVGFEYNGIILNPGGYTHTSVAIGDCIAAITTPVVEVHISNIF
                     AREEFRKISHISAKCAGTITGLGLKSYELALKWLIENK"
     gene            complement(1542..>3033)
                     /locus_tag="PLU36_09720"
     CDS             complement(1542..>3033)
                     /locus_tag="PLU36_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HMZ47069.1"
                     /translation="SEKEKEMERTKEWEKEWDCIKKMREWITTNGIATNEELDQIHEN
                     AKEDVKQQKNNAWQKYQSPIQEFVNEASELINGIMVNDVDKYNYLQQLAKELSATKDP
                     YRRDVLSTLAKAIQTANPLSPGFEEANNYYKKLLQDNANIYNTHLYSETDKSALKVPE
                     VKPFISFDAPSVNGYEVLNKYFDALFESNPKVFAFGEDVGKIGDVNQGFSGLQEKYGS
                     NRISDTGIRELTIMGQGIGLALRGLRPIAEIQYLDYLLFGLQPLSDDVATTHYRTRGI
                     QSCPVIIRTRGHRLEGIWHSGSPMGMIINALRGMYVCVPRNMVQAVGMYNTLLQSNDP
                     GLIIECLNGYRLKEKLPANLLECTVPLGVPEIIKEGNDITIVSYGSTLRIIQEAAERL
                     NIENINCEIIDVQTLLPFDRHHIIIESLKKTNRIVFIDEDVPGGATAFMFREVMEIQG
                     GYKWLDVAPRTITAKAHRPSYGSDGDYFSKPNAEEIMNTIREMMAE"
BASE COUNT          921 a          537 c          406 g         1169 t
ORIGIN      
        1 tttaaataaa catactgtcc accagcttta ggaaacatgg cacttaattc gccatagcta
       61 agggctgcaa ttaatgtcat aaagccagta agcaaccaaa ctaaaattag ccagccagca
      121 ctgcctgtat ttcttacaat atctgcactt actataaaaa ttcctgaacc aatcatacta
      181 ccagcaacaa tcatagttgc atctgttaag cctaaagttg gtttaaatga attattactc
      241 ataaatagtt ttggtattaa aaaatcccat ttgattaatt aaatgggatt tgaagataaa
      301 gattttataa taatacaaag ctaatatatt atttatggtt ttttcttttc tttatagttc
      361 ttatttaagc cttcaattaa atcactagta atgttatatg cagtgtcttt tagataaata
      421 atatctgaac tgctggataa aatgtaagaa aagtttttgc ctttattgta ttcttttaaa
      481 aagtcttcaa ttgttttttt tacatcttga atctttcgga tactttcatc ttgtaactct
      541 tgcgatttta tttgctcttg agcttttaat tcattttcta acctcattat ttcttcattt
      601 gccattgcaa tttcttgttc tgtcatggtg ttaccttttt tttggtactc cataatttta
      661 gatttgcctg cattgcgtaa agaagcaagt tcgccacttt ttctttgttc caaacttctt
      721 aaactgtttc ttacatcttt taaatattca taatggtttt ctatagagtc catttcaaaa
      781 taagctattt taaaaccact ggcatttgct acaactgcat tggtattgct attatttaaa
      841 gaaggagttt ttttagagga gaaatgtaaa aaaaataaaa cccctactgc aatggttaaa
      901 acactaatta atacagttgt aaaattcttc atgaattatt ttttaaatga tataattgtt
      961 tggttattgc ttgttttgaa aagtatttat gggcaaatgt agttttacat taattattta
     1021 tttgttaaaa gagaggctac aaatataata gtgtgctaaa aattaaggtt acttattttc
     1081 aatcagccat tttaatgcta gttcataact ttttaaacca aggccagtaa tagttcctgc
     1141 acattttgca cttatatgag atatttttct aaattcctct ctggcaaaaa tattgctaat
     1201 atgtacttct acaacaggcg tagtaattgc tgcaatacaa tcgccaatgg caacagaagt
     1261 atgtgtgtat ccacctgggt ttaaaattat tccattgtac tcaaatccaa cctgctgcaa
     1321 ttcatttatt aattcgcctt ctacattact ttgatagtaa tgaaaatcaa tatttggata
     1381 ttgtttttct aattgttcaa taaaattctc aaatgtttgg gtgccgtata ttttagtttc
     1441 tcttgagcct aataagttta agttaggccc attgataatt gcaattttca tagtaaaact
     1501 ttcttgaaga aatagatgag ttaatctagt tgatacataa tttattctgc catcatctcc
     1561 ctaattgtat tcattatttc ttctgcatta ggcttgctaa aatagtctcc atcgctgcca
     1621 taacttggcc tgtgagcttt agcagtaata gttcttggtg ctacatctaa ccatttataa
     1681 ccaccttgta tttccattac ttctctaaac ataaaagcag ttgccccacc aggtacgtcc
     1741 tcatcaataa acacaatacg gtttgttttc tttaaagatt ctataattat atgatgcctg
     1801 tcaaaaggta ataatgtttg tacatctatt atttcgcaat taatattttc aatgtttaat
     1861 ctttcagcag cttcttgtat aattcttaaa gtagagccat aagaaacaat tgtaatatca
     1921 ttaccttctt taattatttc aggtacacct aaaggcactg tacactctaa taaattagca
     1981 ggcagttttt cttttaaacg atagccattt aagcattcta taattaagcc tggatcgtta
     2041 ctttgtaaaa gtgtgttgta catgccaaca gcttgcacca tatttcgtgg tacacaaaca
     2101 tacatacctc ttaaagcatt tattatcata cccattgggc taccactatg ccaaatgcct
     2161 tctaatctat gtcctcttgt tctaataata actgggcaac tttgaatgcc tcttgttcta
     2221 tagtgagtag ttgccacatc atcgcttaaa ggttgtaatc caaataacaa ataatctaaa
     2281 tattgtattt cagcaattgg tcttaagcct cttaaagcca aaccaatacc ttgtcccatt
     2341 atagttagtt ctctaatacc agtatcagaa attctgttgc ttccatactt ttcttgtaag
     2401 ccggagaagc cttggttaac atcgccaatt ttgcccacat cttctccaaa tgcaaaaact
     2461 tttgggttag attcaaacaa agcatcaaaa tatttattca aaacttcata accatttact
     2521 gaaggtgcat caaaggaaat aaacggtttt acttctggca cttttaatgc agatttatca
     2581 gtttcgctat ataaatgtgt attataaata ttagcattat cttgtaatag ctttttgtag
     2641 taattatttg cttcttcaaa accaggagaa agtggattgg cagtttgaat tgctttggct
     2701 aaagtactca acacatctct tctgtaagga tcttttgttg cacttaactc tttagctaac
     2761 tgttgtaaat aattgtattt atctacatca tttaccatta taccattaat taattctgaa
     2821 gcttcattta caaactcttg aataggagat tggtattttt gccatgcatt attcttctgt
     2881 tgcttcacat cttcttttgc attttcgtga atttgatcta actcttcatt ggttgctatg
     2941 ccattagttg taatccattc tctcattttc ttaatacaat cccattcttt ttcccattct
     3001 tttgtccgtt ccatttcttt ttccttttcg ctc
//
LOCUS       DAOQPK010000250         3029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_24437_length_3029_cov_3.572966, whole genome shotgun sequence.
ACCESSION   DAOQPK010000250 DAOQPK010000000
VERSION     DAOQPK010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24437_length_3029_cov_3.572966"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="PLU36_09725"
     CDS             complement(<1..746)
                     /locus_tag="PLU36_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47070.1"
                     /translation="MVQVPPPIHGAALRNLSLVESKIINQALHIKLLPLKFAKNIHDI
                     GKLDISKIYKTIAYFFNLIKTIFVFKPNAAYFTLSPTGFAFYRDCLFVLALKICSVKR
                     MYHLRGLGIYNAYTQSKFKATIYKWAFKGAHVICLSKGHCNDVKGLPYASLHVVQNGL
                     KKELENVVVKEFGDEIELLFFSNYVQSKGVLIFIDICEILINKGYSIKGKLVGANGDL
                     SKEDLEVYITSKKLANKIKVCDGVFGKEKF"
     gene            840..1898
                     /locus_tag="PLU36_09730"
     CDS             840..1898
                     /locus_tag="PLU36_09730"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47071.1"
                     /translation="MKKVLKKIQTIYFCIKFIHWKKFFKLYSKNATTILWYYPKYRKN
                     FYTQSIEREFGIINAFIELDIPFKIYFGKKIGQFHNSTIFFMSTVLYDEYKFDNYTTA
                     LSHISKQLEAQGNYVYPTSHEAMFWENKIHMHQQFKEHGINEPKTTFLTKSNLKSQLE
                     LLKFPLLIKEPHSCSSLGIYKISSKAELENLLTNKKLLEQNEAIIAQQLINMRRDMRV
                     TIVGEDDICLHYWRINKSKEWKPTATGYGSDVDFINFPEKWRSHILHTMKKLNLTSCG
                     IDMTFENDDITTEPIFLEVSPFYQPNPPVDISKLDCPYGVYKKQFKLKNSWDKKFVEI
                     IYTIELKLVKYFLNKKPS"
     gene            complement(1891..>3029)
                     /locus_tag="PLU36_09735"
     CDS             complement(1891..>3029)
                     /locus_tag="PLU36_09735"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HMZ47072.1"
                     /translation="VYIQAMLTVFLLIADYGFIVTGTRDISLFEENIEERNKIASAIF
                     FIKALLISVGFIACVVYFIFFYSGSQFVFLLSMAVLISFFMQSFVPSWFFQGIKKNKY
                     ISSINFITKILFFISVFYFLNKGATLIAVPVLEFTSYLIAIIICIYLLYRYEKFKFKF
                     PSLSLIKDQLKQGASIFIISILNWLILAGSIFFVEKYTSKQSEEYGYYAAFSRLAYYI
                     FAIIQPINQAIFPYFVSSFSNKAANASKFVKSTFLYYSILVIVLVVGSILGSYLFFKF
                     FFNQQFNSVLPNYINTFYILIVWVGLVLYNNFISLQLFVSKGKDIVYRNLYLLNTFVT
                     VILFITLIPKYNSIGAAIALLSGELIMLLIYSRKIFVNKEVVLS"
BASE COUNT         1192 a          450 c          375 g         1012 t
ORIGIN      
        1 ttaaattttt ctttaccaaa caccccatca caaactttaa ttttatttgc taattttttg
       61 cttgtaatat aaacttccag gtcttcttta gataaatctc catttgcacc aactaacttg
      121 ccttttatag aatagccctt attaataagt atttcacaaa tatcaataaa aattagcact
      181 ccttttgatt gtacataatt agaaaaaaat aatagctcaa tttcatcacc aaactctttt
      241 acaactacat tttctaattc tttttttagc ccattttgaa caacatgtaa cgaggcatat
      301 ggtagcccct taacatcgtt acaatgccct tttgataaac aaataacatg tgctccttta
      361 aacgcccatt tataaatagt ggctttaaat ttagattgtg tataagcatt ataaatgcct
      421 aaccctctta aatgatacat tcttttaact gaacaaattt ttaaagccag cacaaataaa
      481 caatctctat aaaatgcaaa gccagtaggc gataaggtga aatatgcagc attaggttta
      541 aaaacaaaaa tggttttaat tagattaaaa aaataagcta ttgttttata aattttagat
      601 atatctaatt ttccaatatc atgtatgttt tttgcaaact ttagtggtaa taattttata
      661 tgtaatgctt ggttaattat tttactttca actaaagata ggtttctaag ggctgcacca
      721 tgaattggtg gtggcacttg aaccatgaat aatatttttt tattgtttat ttgcataaat
      781 atttaaaaac ttataaatta gttggaagct ctaactaagg tcaattttaa aaagtaataa
      841 tgaaaaaagt attaaagaaa attcaaacta tttatttctg tataaagttt atacattgga
      901 aaaaattttt caaactatac tcaaagaatg ctacaaccat actttggtac tatcctaaat
      961 acagaaaaaa tttctacacc caatccatag aaagagagtt tggaattatc aatgctttta
     1021 ttgaattgga tattcctttt aaaatttatt ttggaaaaaa aattggtcaa tttcataaca
     1081 gtaccatatt ttttatgtca accgtattgt acgatgaata taaatttgat aactatacca
     1141 cggcattaag ccatatttca aaacagttag aggcacaagg taattatgtt tatcctacct
     1201 ctcacgaagc tatgttttgg gagaacaaaa tacacatgca tcaacagttt aaagagcatg
     1261 gcattaatga acccaaaact accttcttaa caaaatcaaa tttaaaaagc cagttagagt
     1321 tattaaaatt tccattgtta attaaagaac ctcactcttg ttcttcttta ggtatataca
     1381 aaatttcttc taaagccgaa ctggaaaatt tgttaaccaa taaaaaacta cttgagcaaa
     1441 atgaagcaat tattgcacaa caattaatta acatgcgacg agatatgaga gttaccattg
     1501 ttggcgaaga tgatatttgc ttacattatt ggcgtattaa taaatcaaaa gaatggaaac
     1561 ctacagcaac aggttatggt agcgatgttg actttattaa ttttcctgaa aaatggagaa
     1621 gccatatttt acataccatg aaaaaattaa atttaacctc ttgtggcatt gatatgacct
     1681 ttgaaaatga cgacattact acagagccta tttttttaga ggtaagtcct ttttatcaac
     1741 caaaccctcc tgtagatatt tcaaaacttg attgccctta cggtgtttat aaaaagcagt
     1801 ttaaacttaa aaacagttgg gataaaaaat ttgttgaaat tatttatact atagaattaa
     1861 aattagttaa atacttttta aataaaaagc ctagctaagt accacttctt tattaacgaa
     1921 tatttttcta gagtaaatta ataacataat caactctcca cttaacaatg caattgcagc
     1981 accaattgaa ttatattttg gtattaaagt tataaaaagt attacagtta caaaggtgtt
     2041 taagaggtat aaatttctat acacaatatc tttacctttt gaaacaaaga gctgtaaact
     2101 tataaaatta ttatacaata ccaaaccaac ccaaacgatt agtatataaa atgtattaat
     2161 ataattgggt aatacactat taaattgctg attaaaaaag aatttaaaaa ataagtagct
     2221 tcctaaaatg cttccaacca ctaaaactat tactaatata ctataatata aaaaagtaga
     2281 ttttacaaac ttactggcat ttgcagcttt gttggaaaag gaggaaacaa aatatggaaa
     2341 aatagcctgg ttaattggtt gaattatggc aaaaatataa taagccaatc ttgaaaatgc
     2401 tgcataatag ccgtactctt cagattgttt acttgtatat ttttctacaa aaaaaatact
     2461 gcctgcaagt attagccaat ttaaaattga aatgataaaa atacttgcac cttgttttaa
     2521 ctgatctttt attagcgata atgacggaaa tttaaattta aatttttcat acctgtataa
     2581 caaataaata catataatta tagcaattaa ataagaggta aattctagaa caggtactgc
     2641 tattagcgtt gctcccttgt ttaaaaaata aaatacactt ataaaaaaca atattttggt
     2701 aataaagttt atagacgaaa tgtacttatt ttttttaatg ccttgaaaaa accaggatgg
     2761 aacaaagctt tgcataaaaa aagaaataag aactgccatg gataataaaa acacaaattg
     2821 actaccagaa taaaaaaaaa taaaataaac aacacaggca ataaacccta cactaattaa
     2881 taacgctttt ataaaaaaaa tagcagaagc aattttgtta cgttcttcaa tattttcttc
     2941 aaacaaacta atatctctgg tgccagttac aataaagcca taatcggcaa ttaataaaaa
     3001 aacagtaagc attgcctgta tatacacta
//
LOCUS       DAOQPK010000251         3003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_24825_length_3003_cov_3.206581, whole genome shotgun sequence.
ACCESSION   DAOQPK010000251 DAOQPK010000000
VERSION     DAOQPK010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24825_length_3003_cov_3.206581"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="PLU36_09740"
     CDS             complement(<1..1084)
                     /locus_tag="PLU36_09740"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HMZ47073.1"
                     /translation="MFQKIIFIFIAFVAVCTAKAQKKIAPDRDTLQAGTLLNIIYAER
                     MNFKKEDSLNQFVSLAGNVQIKQGKTFFYADSAVLNQYLNILEAFGKVHINDADSIHT
                     YANYLKYIGKEKKAYLKGNVKLTDGKGILTTDELEYDVTTKIGTYLKGGKVVNGKTVL
                     TSKQANYYGETKDVYFINKVVLKDPDYTINTDTLLYNTYTNITTFVTATTILSGKTKI
                     KTRKGYYDLNKKKAYFGERPEIEDSTGTLKADEVAIDDSTGKSEFKGNVVYKGKDSTS
                     GFDIIANKVERDSKKGSIRATQAPILLIKQGKDSVFIRADTLFSAKLSDLLLERNVHD
                     LRDSSEGKLYKIPSTDKDTTSDKYFEA"
     gene            complement(1088..2086)
                     /locus_tag="PLU36_09745"
     CDS             complement(1088..2086)
                     /locus_tag="PLU36_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HMZ47074.1"
                     /translation="MKISNETKIGVLAVISIVLLIIGFNFLKGKSVFKTGNFIYANFT
                     NTKGILVSNPVLANGYRIGTVYEIVENDKSLQSIKVAIKLKKEYNIPSDSYAMIEANP
                     LGVTSIVIKLGSSKSLLTHNSTITTKENEGLITQLSNTITPTVDQVKNTFHTLDSTLK
                     NINSILDATTKNNLQVTVANINKLTASLLVSSANIEKMLVAQSGSIAQTMDNVNSFSK
                     NLADNNSKITNTIQNLETATGKLANSNIDNAVNSLQQAVDKMNTTLTKLNSSEGSLGL
                     LLNDKTLYNNLTNTVRSANILIDDLKTHPKRYVNISVFGKKDKSTPLKAPLSETKQ"
     gene            2234..>3003
                     /locus_tag="PLU36_09750"
     CDS             2234..>3003
                     /locus_tag="PLU36_09750"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HMZ47075.1"
                     /translation="MKSFLIGVVLFFSFHSNGSTISGFIKDSKGNILPYASILISGTT
                     KGTTANGKGYYSLQISPGSYTIVCQHIGFKSTEKHIEIRNTPITENFILEPQSYNLLD
                     VTVKTGGEDPAYEIIRNAIKNREKNLNEIRKFECEVYLKGQLQLGDYPKKFLGQKVDF
                     EDGDTSKKKMLFLSETIAKYSVEKPNDAKIEVVSTRVSGNSDGFGFSFPQIISFYENN
                     VQIGRGLNPRGFISPISHNALNYYKYKFMGTFFENGKEV"
BASE COUNT          973 a          425 c          448 g         1157 t
ORIGIN      
        1 aagcttcaaa atatttatcg cttgttgtgt ctttgtctgt actgggtatt ttatataatt
       61 ttccttcgga agagtctctt aaatcatgta catttctttc taataataag tcagataatt
      121 ttgcagaaaa taaagtatct gccctaataa aaacgctatc ctttccttgt ttaattaata
      181 aaataggagc ttgtgttgct ctaatacttc cttttttact atctctttct actttatttg
      241 caataatatc aaagccacta gttgaatctt ttcctttata aactacattg cctttaaatt
      301 cagattttcc tgtgctgtca tcaatggcaa cttcatctgc ttttaaggtt cctgtactat
      361 cttcaatttc aggtctttcg ccaaaatagg cttttttctt gtttaaatcg taataacctt
      421 ttcttgtttt aatttttgtt tttccagaaa ggatagtggt tgctgttaca aaagtggtaa
      481 tatttgtata agtattatac aataaagtat ctgtattaat ggtataatct gggtctttta
      541 aaactacttt attaataaaa taaacatctt tggtttctcc ataataattt gcttgcttgc
      601 tggttaaaac tgttttacca tttacaactt ttccaccttt taagtaagta ccaatttttg
      661 tagttacatc atattctaac tcatcagtag ttagtatgcc tttgccatcg gtaagtttta
      721 cattgccttt taaataagct tttttttctt taccaatata ctttaaataa tttgcgtaag
      781 tatgaatact gtctgcatca ttaatgtgca ccttgccaaa agcttctaga atatttaagt
      841 actgatttaa aactgcacta tctgcataaa aaaaggtttt accttgttta atttgtacat
      901 tacctgctaa agaaacaaac tgattgagtg aatcctcttt tttaaaattc attctttctg
      961 cataaataat attcaataga gtaccagctt gtaatgtatc tctatcgggt gcaatttttt
     1021 tttgagcttt ggcagtacaa acagcaacaa atgcaataaa tatgaaaatt attttttgga
     1081 acatttatta ttgcttggtt tcagaaagag gagcttttaa tggtgtactt ttatcttttt
     1141 taccaaatac cgaaatattt acatagcgtt taggatgtgt ttttaaatca tcaattaaaa
     1201 tattagcact acgtacagta ttagttaaat tattgtacaa ggttttatcg ttaagtaaca
     1261 gtcctaaact accctcactg ctatttaatt tggttaaggt tgtgttcatt ttatctactg
     1321 cttgttgtaa actgtttaca gcgttatcaa tatttgagtt ggctaacttt ccagttgcag
     1381 tttctaaatt ttgtatggtg ttggtaattt ttgagttgtt atctgccaaa tttttagaaa
     1441 aactgtttac attatccata gtttgtgcaa tgctaccact ttgtgcaacc aacatttttt
     1501 cgatattagc agagcttaca agtaaagatg ctgtaagctt atttatattg gctactgtta
     1561 cttgtaaatt atttttagtt gtagcgtcca aaatagaatt gatatttttt agagttgaat
     1621 ctaaagtgtg aaaagtattt ttcacttgat ctacagtagg cgtaatggta ttggaaagtt
     1681 gagtaattaa gccttcattt tctttagtag taatggttga attatgtgtt aataaacttt
     1741 tactactgcc taattttatt acaatacttg ttactcctaa agggtttgct tcaatcattg
     1801 cataagaatc ggatggtata ttgtattctt tttttaattt aatggctacc ttaatgcttt
     1861 gtaaggattt atcgttttct actatttcat aaacggttcc tattctatat ccattagcta
     1921 aaacagggtt agaaaccaaa atgcctttag tgttggtaaa gttggcataa ataaaattac
     1981 cagttttaaa aacacttttt ccttttaaaa aattaaatcc tataattaat aacacaatag
     2041 aaataacagc taatactcca atcttggttt cgttagaaat tttcatgtag caaatctaag
     2101 ggttaaaaag tagtggtcaa atggttaacc ttgttaaaga ttaaaaaatt atattttaat
     2161 aaatgctaaa attaacattt tcaatttttt gtaacttttt gttatttatt ttcgttttta
     2221 cacaaaatta tttatgaagt cttttttgat aggggttgta ttgttttttt cttttcattc
     2281 taatggctct acaataagtg gttttattaa agattctaaa gggaatatac taccttatgc
     2341 atcaatactt attagtggaa caaccaaagg cactactgcc aatggtaaag gatattattc
     2401 attgcaaata tcacctggta gttatacaat tgtttgccaa catatagggt ttaagagtac
     2461 tgaaaaacat attgagatac gaaatacacc cataactgaa aattttatat tagagccaca
     2521 atcttataac ctgttagatg ttacagtaaa aacaggaggc gaagatcctg cctatgaaat
     2581 aattagaaat gctataaaaa atagagaaaa aaatttaaac gaaataagaa agtttgaatg
     2641 tgaagtttac ttaaaaggcc aactacaatt gggcgattat cctaaaaaat ttttggggca
     2701 gaaagtagat tttgaagatg gagataccag caaaaaaaag atgctttttt taagcgaaac
     2761 aattgccaaa tattctgtag aaaaaccaaa tgatgctaaa atagaagtag tctctaccag
     2821 agttagtggt aatagtgatg gtttcggttt tagttttcct caaattattt ctttctacga
     2881 aaacaatgta caaataggta gagggctaaa cccaagaggg tttatttcgc ccattagcca
     2941 taatgccctt aattattata aatataaatt tatgggtact ttttttgaga atggaaaaga
     3001 agt
//
LOCUS       DAOQPK010000252         3001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_24850_length_3001_cov_4.921928, whole genome shotgun sequence.
ACCESSION   DAOQPK010000252 DAOQPK010000000
VERSION     DAOQPK010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24850_length_3001_cov_4.921928"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..1173)
                     /locus_tag="PLU36_09755"
     CDS             complement(199..1173)
                     /locus_tag="PLU36_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HMZ47076.1"
                     /translation="MSKGPVSKFIQHHYRHFNAAALMDAAKGYETHLTEGGKMMITLA
                     GAMSTAELGISLAEMIRQDKVHIISCTGANLEEDVMNLVAHSHYKRVPNYRDLTPQDE
                     WDLLENHYNRVTDTCIPEEEAFRKLQKHLYKQWKDAEANGERFFPHEFLYKTVLSGDL
                     KQYYEIDAKDSWIIAAAEKNIPIVCPGWEDSTTGNIFASYVIKGELKASTVKSGIEYM
                     VWLTEWYRNNSSGKGVGFFQIGGGIAGDFPICVVPMMYQDLEWHDVPFWSYFCQISDS
                     TTSYGSYSGAVPNEKITWGKLDINTPKFIVESDATIVAPLIFAWILGW"
     gene            complement(1254..1787)
                     /locus_tag="PLU36_09760"
     CDS             complement(1254..1787)
                     /locus_tag="PLU36_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HMZ47077.1"
                     /translation="MSSLLKNIIRFILFVLVQVYILNQVPPLHKYFVPYIYFLYILWL
                     PFNINRLFLLVVAFVYGLTVDFFTGWYGLYTAPTVLIAYLRPFLLNLLIPQETTEQSY
                     IEPSIRSMGATPYSLYIVLLTFIHQFYFVLIEWLQFGSFNLFIGKVAGSTVLSLLLIF
                     IAELLFFRKAKYRTNAA"
     gene            complement(1780..2628)
                     /gene="mreC"
                     /locus_tag="PLU36_09765"
     CDS             complement(1780..2628)
                     /gene="mreC"
                     /locus_tag="PLU36_09765"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HMZ47078.1"
                     /translation="MKNIFLFIKRYFVLICFLILQVICIVMLSNSSKTHEAFFASTAN
                     ELTGPINKKYYGIKEYFSLKKINKQLNDENAQLRNLLKTNFEVPDSSVITQKDTLLKD
                     TLNQYRKYTYMPARVVGNTISSNTNYLMLERGSKQGVEKNMSVVSPQGVVGIVVEVSE
                     NYCRVMSLLHKNTRISAMLKKNKSMGDVEWNGEDPHFVTMRKVSKSANVIVGDTVVTS
                     TYSSNFPSNIMIGKVVQVKPDVSTSFNNVTVKTATDFFNIQYVYIIKNIYYHEQEKLL
                     NKRTHE"
     gene            complement(2697..>3001)
                     /locus_tag="PLU36_09770"
     CDS             complement(2697..>3001)
                     /locus_tag="PLU36_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HMZ47079.1"
                     /translation="IPKQIMVSYQEIAEALDKSIFKVEEAILKALETTPPELAADIYR
                     RGLYLTGGGALLRGLDKRLSAKIKLPVHIADSPLKSVVRGTGIALKNYQRYPFIMK"
BASE COUNT          983 a          519 c          423 g         1076 t
ORIGIN      
        1 ttccattgta atttctgtct gtgttatcgc ttcgataaag ttttgaataa gggccacaac
       61 tatgaattat tgtcaataca aaaattgcta aaaggaaagt gtgaaaattt cgtttcattt
      121 caaaaaatta aaaattttcc aagatggcta ccaattttcc tttatcagat acagttatac
      181 ataatttttc tttctatttt accagcctag tatccaggca aatattaatg gtgcaactat
      241 agtagcatca ctttctacaa taaatttagg agtattaata tctaatttac cccaagtaat
      301 tttttcgttt ggcacagcac cactataaga gccataagat gtagtgctgt cgcttatttg
      361 acaaaaatag ctccaaaaag gcacatcgtg ccattctaaa tcttggtaca tcataggtac
      421 aacacaaatt ggaaaatcgc cagcaatacc accaccaatt tgaaaaaaac caactccttt
      481 accagatgag ttatttctat accactctgt tagccaaacc atatattcaa taccactttt
      541 aactgtgctt gcttttaact ctccttttat tacatagctt gcaaaaatat ttcccgttgt
      601 gctgtcttcc caacctgggc aaacaatagg aatatttttt tcggcagcag caattatcca
      661 actgtcttta gcatcaattt cataatactg ttttaaatcg ccacttaaaa ctgttttata
      721 taaaaactca tgagggaaaa atctttcgcc atttgcttca gcatctttcc attgcttata
      781 taaatgtttt tgtaattttc taaatgcttc ttcttctggt atgcaagtat ctgtaactct
      841 attgtaatgg ttttctaata aatcccattc atcttgtggc gttaaatctc tataattagg
      901 tacacgtttg taatgagagt gagcaacgag gttcattaca tcttcttcta aatttgctcc
      961 tgtgcaacta ataatatgta ctttatcttg cctaatcatt tcggctaatg aaatacccaa
     1021 ttctgcagtg ctcattgcac ctgccagggt tatcatcatt tttccacctt cggttaaatg
     1081 ggtttcatat cctttagcag cgtccattaa agcagctgca ttaaaatggc gataatggtg
     1141 ttgtataaat ttagaaacag gccctttact catgattaaa tattttatag ttagttggca
     1201 aaagtagttt tttacttggt attaaataaa acaaaaatga atgctttaat tatttatgca
     1261 gcattggttc tatactttgc ttttctaaaa aaaagtaact cggcaataaa aattaataat
     1321 aaacttaaaa ctgtgctgcc agctactttt ccaataaata aattaaagct gccaaactgt
     1381 aaccactcta ttaaaacaaa ataaaactgg tgaatgaaag ttagtaaaac aatatacaaa
     1441 ctatatggcg ttgcacccat acttctaatg cttggttcaa tataactctg ctcagttgtt
     1501 tcttgtggta taagtagatt taataagaag ggacgtaagt aagcaattaa aacagttggt
     1561 gctgtatata aaccatacca gccagtaaaa aaatctacag ttagcccata tacaaaagct
     1621 actacaagta aaaataatct gttgatatta aaaggcaacc ataaaatata taaaaagtaa
     1681 atatagggta caaagtattt atgtaagggt ggtacttgat taagtatgta cacttgaacc
     1741 aaaacaaata gtataaagcg tattatattt tttaacaggc tactcatgtg ttcttttatt
     1801 taaaagtttt tcctgttcgt gataatatat atttttaata atatacacat actgaatatt
     1861 aaaaaaatct gttgctgttt taactgttac attattaaaa ctggttgaaa catctggttt
     1921 aacttgtacc actttaccaa tcataatatt ggatggaaaa ttgcttgaat aagtgctggt
     1981 aactacagta tcaccaacaa taacattggc agatttgcta actttacgca tagttacaaa
     2041 atgtgggtct tctccattcc attcaacatc tcccatgctt ttattctttt taagcatagc
     2101 actaattctg gtgtttttat gtaataagct cattaccctg caatagtttt cgcttacttc
     2161 aacaacaatt ccaacaacac cttgcggcga tacaacactc atgttttttt ccactccttg
     2221 tttgcttcct ctttctaaca ttaagtaatt ggtgttagag gaaatagtat taccaacaac
     2281 ccttgcaggc atataagtat attttctata ttggttgagt gtatctttta agagtgtatc
     2341 tttttgtgtg ataactgaag agtctggcac ttcaaaattt gtttttagca aatttctaag
     2401 ctgtgcattt tcatcattta attgtttatt aatttttttg agagagaagt attctttaat
     2461 gccgtagtat tttttattta ttggccctgt tagttcattt gcagtagatg caaaaaatgc
     2521 ctcgtgtgtt ttactgctgt tactaagcat aactatacaa ataacctgca atattaaaaa
     2581 gcagattaga acaaagtaac gtttaataaa aagaaaaata tttttcacac ttagcagcag
     2641 tttttgaaat aatacatttg ttttaacttg ttaaatcaaa agtactatct cattttctat
     2701 ttcattataa aaggatatcg ctggtagttt tttaatgcaa ttccagtacc tcttacaacg
     2761 ctttttaaag gagagtctgc aatatgtaca ggtaatttta ttttagcact taatctttta
     2821 tctaagccac gtaataaagc acctccacca gttaaataca accctcgtct ataaatatct
     2881 gcagcaagtt ctggtggtgt ggtttctaaa gcttttaaaa ttgcttcttc tactttaaaa
     2941 atacttttat ctaatgcttc tgcaatttct tgatagctta ccataatttg tttaggaata
     3001 c
//
LOCUS       DAOQPK010000253         2999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_24889_length_2999_cov_3.927989, whole genome shotgun sequence.
ACCESSION   DAOQPK010000253 DAOQPK010000000
VERSION     DAOQPK010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24889_length_2999_cov_3.927989"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1698
                     /locus_tag="PLU36_09775"
     CDS             <1..1698
                     /locus_tag="PLU36_09775"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HMZ47080.1"
                     /translation="NGKEVSRIKVIPKRKYEPLFAGYISIIENEWRIFSTELYLLKEQ
                     QMQLLDTLHIQQMYVPIKDKWVIKQQVIYPAGKLLGFKFFGSFVQVYSNFNINPTFKK
                     KHFDNVLLKVNDSANKKAKHYWDSIRPVPLLEEEAIDYVKKDSLEQVRKDPKYLDSLD
                     KKNNKFSLLGLLLTGKTFQKRKTKESIAIEPLFFKINYNTVEGWNLKLSPTYTKRYEG
                     RRSITISPNLIYGFNNKHFNAFLSTRYIFGKKYVNNLSFSAGKAVLQYNNNNPIEERD
                     NTFSTLYYTKNYLKIYETWFTKINYRVGIGKGFDLTTNFQYQDRLKLDNLTKIEQWKQ
                     FGNRIFTPNYPVELGAASVKSHKAAIASFGIQFRPGSKYVELPGRTLNLGSKYPVISA
                     NLTFGIKGLLNSNVNFSKWNFDISDDINLKLGGVVKYKFNVGGFLNAKTVYLQDYQHI
                     QGNRTVTATQYLNSFQLAPYYAFSNTSSLMTSAHIAYHLNGFLTNKIPVFKKWNCFFV
                     LSGSALYIKPNLHYYETMFSIENIFKIIRIDLVQGFGDNNYKTSGIRLSLPGFLTGN"
     gene            1725..2918
                     /locus_tag="PLU36_09780"
     CDS             1725..2918
                     /locus_tag="PLU36_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ47081.1"
                     /translation="MTVIGLIKEGKIPSDNRVALTPAQCKWLKINLPNVKILVESSAT
                     RCFSDAEYKQAGIEVTNDLTSCDILLGIKEVPVDMLIANKTYLFFSHTKKQQPHNRKL
                     IKAIIEKNITLIDYECLEHDDGQRIIGFGFFAGIVGAHNGIMAFGNRTGLFQLPRVYQ
                     CKDYRSLIHHYFGLKLPNIKIAVTGSGRVAHGIIEVMNLMGVHEVEADEYLEKKFSYP
                     VYVQLKGADLYTHQENKTYKREHFHQNPTEYRSRFLPYTKCTDILMNGVYWDENIPRL
                     FELTDVNERFVTTTIADITDDEHGSVPLNLGDQTIENPVYGVDKKTFLKTEPYLPSSI
                     DIVAVGNLPNELPRDASRYFGEQLLKYILGDILNGASDIITRATIINQGKLTKEFSYL
                     ENYVK"
BASE COUNT         1157 a          383 c          489 g          970 t
ORIGIN      
        1 aatggaaaag aagtaagtag aattaaagta attcctaaaa gaaaatacga accattattt
       61 gcaggttata ttagtattat tgaaaatgaa tggagaattt ttagtacaga gttatattta
      121 ctaaaagagc agcaaatgca gttgttagat acattacata tacaacaaat gtatgttcct
      181 ataaaagata aatgggttat taagcagcaa gtaatttatc ctgctggtaa attattaggt
      241 tttaaatttt tcggcagctt tgttcaagtt tattccaatt ttaatattaa tcctactttt
      301 aaaaagaaac attttgataa tgtactttta aaagtaaatg atagtgccaa caaaaaagct
      361 aaacactatt gggatagtat taggccagta ccattgttag aggaagaagc aatagactat
      421 gtgaagaaag atagtttaga gcaagtaaga aaagacccta aatatttaga ttcattagat
      481 aaaaaaaata acaaattttc tctattaggc cttttattaa caggaaaaac ttttcaaaaa
      541 agaaaaacaa aggaaagtat agccattgag cctttatttt ttaagattaa ttataataca
      601 gtagaagggt ggaatttgaa gttatctcca acttatacaa aaagatatga aggaagaaga
      661 tcaattacta tttcaccaaa tttaatatac ggtttcaata ataaacactt taatgccttt
      721 ttaagtacaa ggtatatttt tggaaaaaaa tatgtaaaca atttgtcttt ttctgcaggt
      781 aaagcagtct tacaatataa taataacaac cctattgagg aaagagataa tactttttct
      841 accttgtatt acaccaaaaa ttatttaaaa atttatgaaa cctggtttac taaaataaac
      901 tatagagttg gtattggtaa aggatttgat ttaactacca actttcaata tcaagataga
      961 ttgaaattag ataatttaac taaaattgag caatggaaac agtttggaaa tagaattttt
     1021 acccctaact atcctgtaga attaggtgca gcaagtgtaa aaagtcataa agctgcaata
     1081 gcaagttttg gaatacagtt tagaccagga tctaaatatg tagaactgcc aggtagaaca
     1141 cttaacttag gttctaaata ccctgtgatt agtgcaaact taactttcgg aattaaaggc
     1201 ttattaaata gcaacgttaa ttttagtaaa tggaattttg atattagcga tgatataaat
     1261 ttaaaattag gtggcgtagt aaaatataaa tttaatgtag gtgggttctt aaatgccaaa
     1321 actgtttatt tacaagatta tcagcatata caaggtaaca gaactgtaac tgctacacaa
     1381 tatttaaaca gctttcagtt agctccttat tacgcattca gcaacacttc ttcgttaatg
     1441 acttcagcac atatagctta tcatttaaac ggatttttaa ctaacaaaat tccggtattt
     1501 aaaaaatgga attgcttttt tgtattatct ggcagtgcgt tgtatataaa acccaattta
     1561 cattattatg aaaccatgtt ttctattgaa aatattttta aaattattag gatagatttg
     1621 gtgcaaggtt ttggcgataa caattataaa acaagtggta ttaggttaag tttacctggt
     1681 tttttaactg gtaattaatt tatattgcag cccaaaagac aagaatgact gtaattggat
     1741 taattaagga aggaaaaata ccatcagata acagagttgc attaacacca gcacaatgta
     1801 aatggttaaa aataaattta cctaatgtaa aaattttggt agaaagtagt gctaccaggt
     1861 gttttagtga tgctgaatat aaacaagctg gaattgaagt aaccaacgat ttaactagct
     1921 gtgatatact tttaggcatt aaagaggtac cagttgatat gcttattgca aacaaaacct
     1981 acttattttt cagtcatacc aaaaagcaac aaccccataa tagaaaacta ataaaagcta
     2041 ttattgaaaa aaatattacg ctgatagatt atgaatgttt agaacatgat gatgggcaaa
     2101 gaattattgg ttttggtttt tttgcaggta ttgttggtgc ccataatggc attatggctt
     2161 ttggcaatag aacaggcttg tttcagttgc caagagtgta ccaatgtaaa gattatcgtt
     2221 ccttaataca tcattatttt ggattaaaac ttcctaatat aaaaattgcc gttacaggta
     2281 gtggtagagt tgcacatggt attatagaag taatgaattt aatgggtgtt catgaagtag
     2341 aggcagatga atatttagag aaaaagttta gttacccagt gtatgtgcag ttaaaaggtg
     2401 cagatttata tacacaccaa gagaataaaa cttataaaag agaacatttt catcaaaacc
     2461 ctacggaata cagatcaaga tttttgccat atactaaatg cactgatatt ttaatgaatg
     2521 gagtttattg ggatgaaaat ataccaagac tatttgagct tacagatgta aatgaacgct
     2581 ttgtaactac aacaattgcc gatattactg acgatgaaca cggctcagtg ccattaaatt
     2641 taggagatca aactattgaa aatcctgtat atggcgttga taaaaaaact tttcttaaaa
     2701 ccgaacctta ccttccttca tccatagata ttgtagcagt tggaaatttg cccaatgaat
     2761 taccaagaga tgcaagcaga tattttggag agcaacttct aaaatatatt ttaggagata
     2821 ttctaaatgg agctagcgat attattacaa gagcaacaat aataaaccag ggaaaactaa
     2881 ccaaagaatt ttcttatctt gaaaattatg taaaataaag tttaattatt tagttatact
     2941 tttaatgaat aatcaaagta ttcaattact cagctcaaaa aataaaaaag aattttctt
//
LOCUS       DAOQPK010000254         2976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_25236_length_2976_cov_4.707977, whole genome shotgun sequence.
ACCESSION   DAOQPK010000254 DAOQPK010000000
VERSION     DAOQPK010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25236_length_2976_cov_4.707977"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PLU36_09785"
     CDS             <1..435
                     /locus_tag="PLU36_09785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ47082.1"
                     /translation="SWHFGDGDSLVTNNINASVLHQYNETKLFNACLIVSNSYNCKDT
                     SCNPIQARVVPKVGVPNAFTPNGDGINDKIFVQGFGIQKMTWRIYNRWGKLLFTGISL
                     GRGWDGKFNGEIQPQDVYNYVLDVKFSDGTTQVIKGDFTLLK"
     gene            454..1482
                     /locus_tag="PLU36_09790"
     CDS             454..1482
                     /locus_tag="PLU36_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HMZ47083.1"
                     /translation="MSKYFKILFFLFTTFVVQKSHAQDLHFSQYFNAPLLINPANTGF
                     NPDFDYRVGGNYRNQWSSISNNPYKTMSIWADAQLFTERFDESWVGVGGAFLQDVAGS
                     GNLTSTRAYASIAYHQMLGYNGLLSAGFNIGFTQKRIDLSKLTFNNQWNGNFFDISIP
                     SNEPFVYSSTSYFNLQAGLNYALFVNDNVYLSTGISVSNINKPNESFFNKNNINTKVN
                     SRYTFFADATIRVNNVWILNPNFYYSTIGTAKEIVLGGTLQRDLSAENNGSLQFLLGG
                     YYRLSDAMVPVIGFDVKNFKVTFSYDATSSSLKNYNQSRGAYEVSIVKSGLYKGKDKN
                     IKCPRVRF"
     gene            complement(2015..2503)
                     /locus_tag="PLU36_09795"
     CDS             complement(2015..2503)
                     /locus_tag="PLU36_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HMZ47084.1"
                     /translation="MNIHFTAFKKVCSNRLKLNLFLLTKLPMVFFSGIKLKEFSNEKS
                     ITTVNYKWINQNPFNSIYFAVLSMAAELSTGLLAFGNIYKRKPKVSMLVIKMESFFYK
                     KAVGKISFICKDGLAIEEAIEKAIAYNNATPITCTSIGINEQNEIVAKFIIEWSFKSK
                     PR"
     gene            complement(2543..>2976)
                     /locus_tag="PLU36_09800"
     CDS             complement(2543..>2976)
                     /locus_tag="PLU36_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47085.1"
                     /translation="RLERGLYLTFGKAYSVMRIILIPLFNLIQAYSNIDIHYKADIRG
                     GLLVLHPSVGIVISAFADIGKNLTLTGGNIIGAREGCKLGELKIGNECTLGANAVVLG
                     PINLGNYINIGASACVVKDCVIDGTSLIGVPAKLIPKQPVI"
BASE COUNT         1080 a          460 c          439 g          997 t
ORIGIN      
        1 tcttggcatt ttggcgatgg tgatagcttg gttactaata atataaacgc tagtgtttta
       61 caccaatata atgaaacaaa attatttaat gcttgtttaa tagtgagcaa tagctataat
      121 tgtaaagata caagctgcaa ccctattcag gcaagagtag tgccaaaagt aggcgttcca
      181 aatgcattta caccaaatgg cgatggtata aatgataaaa tatttgtgca aggatttggc
      241 atccaaaaaa tgacttggag aatttataat cgttggggga aattgctttt tacaggcatt
      301 agtttaggta gaggttggga tggcaaattt aatggtgaaa tacagccaca agatgtttac
      361 aactatgtgc ttgatgtaaa gtttagcgat ggtacaacac aggttataaa aggcgatttt
      421 actttattaa aataatcttt gaggttcaat taaatgtcta aatattttaa aatattattt
      481 tttcttttta caacatttgt tgtacaaaaa tctcatgctc aggatttaca tttttctcaa
      541 tactttaatg ccccattact tataaatcct gccaatactg gctttaatcc agactttgat
      601 tatagagttg gaggtaatta tcgcaaccaa tggtcatcta tatctaacaa cccatataaa
      661 actatgagta tttgggcaga tgcacaattg tttaccgaaa gatttgatga atcatgggta
      721 ggtgttggag gtgcattttt acaagatgtc gcaggttcgg gtaatttaac ttctacaaga
      781 gcttatgcat ctattgccta ccatcaaatg ttaggttata atggtttatt aagtgcaggc
      841 tttaatattg gatttacaca aaaaagaata gacttatcca aacttacttt taacaatcaa
      901 tggaatggta atttttttga tatcagtatt ccttctaacg aaccttttgt atatagttca
      961 acatcttact ttaatttaca agcaggttta aattatgctt tgtttgtaaa tgataatgtt
     1021 tatttaagca caggaattag tgtaagtaat attaataaac ccaacgaatc tttctttaat
     1081 aaaaataata ttaatacaaa agttaattca cgttatacat tttttgcaga tgctacaatt
     1141 agggtaaata atgtttggat tttaaacccc aatttttatt atagcaccat tggtactgcc
     1201 aaagaaattg ttttaggagg aacattacaa agagatttaa gtgccgaaaa taatggcagt
     1261 ttgcaatttt tattaggagg ttactataga ttaagtgatg caatggtacc tgtgataggt
     1321 tttgatgtaa aaaattttaa agtaactttt agttacgatg ctacatcctc ttctcttaaa
     1381 aattacaatc aaagtagggg tgcttacgaa gtatctattg taaaaagtgg cttatataaa
     1441 ggaaaagata aaaatataaa atgcccaaga gttagatttt aaagctattc aatgtctatc
     1501 cacaattatt ctttacaaaa aactagtgct ttcacttgct aaaagttagt aaaagtacga
     1561 agcctaaaag tatattttcc ttgctcattt tcagtaaact gaatgtctaa aaaagagaag
     1621 ttttgctctt gttttgggaa taaactgtgt atatgtttgc atagtcaatt tgatacaatg
     1681 gttcacccgt ttttaattca aatatccaga aaaatcaaag tagtataaag aaaacaaaca
     1741 acaacaacat ccgataccta agaaaacaga aacctacgta ttaaaaaaga caacataaat
     1801 ccaaggatct aagaaaagct gaacccgttt ttaaaaaatc caaagtgtga aaaaccaagg
     1861 tacttgtagc ctaaattaca agagaaaaaa aacaatattc taaaaagaaa gacagaaaat
     1921 gtccaacatt cctgagaaac aaaaagactc aggttgaatt tgaaccaggg tctgatactt
     1981 atcagacctt tttttatttt aaaaactgat tatactatct aggtttagat ttaaagctcc
     2041 actcaattat aaactttgct acaatttcgt tttgctcgtt aataccaata gatgtgcaag
     2101 taattggggt tgcattatta taagctatag ctttttcaat tgcctcttct atggccagcc
     2161 catccttaca aataaaagaa attttcccaa ctgctttttt gtaaaaaaaa gattccattt
     2221 taattactag catacttact ttaggttttc ttttataaat attaccaaat gcaagtaaac
     2281 cagtactaag ctcggcagcc atgcttaaca cggcaaaata aattgaatta aatgggttct
     2341 ggttaatcca cttgtaattt acagttgtaa tagatttttc attactaaat tctttcagtt
     2401 taattccact aaaaaatacc attggtagtt tagtaagtaa aaataaattt aactttaatc
     2461 tgttgctaca tactttttta aatgcagtaa aatgtatatt cacaaatcaa tttttgctta
     2521 aaatgaacta aaataaagta gtttatatta caggttgttt aggtattaac tttgcaggta
     2581 caccaatcaa acttgtgcca tcaataacac aatctttaac cacacaagca gaagctccaa
     2641 tattgatata attacctaaa ttaattgggc caagaaccac tgcattagca cctaaagtac
     2701 attcattccc aattttcaac tcacctagtt tacaaccttc tcttgcacca attatattac
     2761 cacctgttaa ggttaaattt ttacctatat ctgcaaatgc agaaataaca atacctacag
     2821 atggatgtaa aaccaataac cctcccctaa tatcagcttt ataatgaata tctatattag
     2881 aatatgcttg aattaaatta aacaacggaa ttaaaataat tcgcattaca ctatatgctt
     2941 ttccaaaagt taaatacaag cccctttcta acctat
//
LOCUS       DAOQPK010000255         2970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_25329_length_2970_cov_4.374614, whole genome shotgun sequence.
ACCESSION   DAOQPK010000255 DAOQPK010000000
VERSION     DAOQPK010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25329_length_2970_cov_4.374614"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLU36_09805"
     CDS             complement(<1..188)
                     /locus_tag="PLU36_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47086.1"
                     /translation="MKLKLSQRIAISYYRAKIKTISLLSKKLAAEKALTIFCTPFSGK
                     PKRAIPHLFHQAEKLVCHT"
     gene            208..972
                     /locus_tag="PLU36_09810"
     CDS             208..972
                     /locus_tag="PLU36_09810"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HMZ47087.1"
                     /translation="MNVVMIGSGKVAALLSKKIHNAGLKVLQVYSPNLQHATLLADII
                     NAEATNNIENIIQSANIYIIAIADDSIEGVASKLNVSKKALVLHTSGAVNMNVLKSCS
                     DFYGVLYPIQSISKTTDNNAVLPFAIDANNDIAMETIKFITNALHSNVEILNDLQRLH
                     LHIAAVFVNNFVNFLYFNASNICKDATINFNVLQPLIEETALRLRNNLPEVVFTGPAI
                     RKDFSTINKHLKVLETNEQLFELYYFLTKCILVKES"
     gene            complement(1077..>2970)
                     /locus_tag="PLU36_09815"
     CDS             complement(1077..>2970)
                     /locus_tag="PLU36_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HMZ47088.1"
                     /translation="HGTKKLQADLFTEMKSRIKEKDESVPTFKNGYYYYSKTEDGKQY
                     FKYCRKKGSLDAPEEILLDVDKMAEGKAYFSVNGFSVSSNNKLLAFGVDEVSRRQYTI
                     YIKNLETGELLKDVIPNTEGSACWAEDNTTIFYTAKHPVTLLSEKIKKHVLGTDAAKD
                     VVVYHEKDNSNYIGISKTKNDKYIYIYSGGTLSSEIFYIDAAKPNDVFKSFQPRIKNV
                     LYSIYALEDKFLILTNNNAINFKLMECPLDKTGIEHWKELVPHRPDILLQDVDVFKNF
                     IVLSERKNGLTQINIKNTKENKEHYLDFGEPTYAAYIGSNEEYNTDKLRYNFTSLTTP
                     NSVFDYDMNSKNKTLLKQQEVLGGYNSKDYVSERLFATAKDGTKIPISIVYKKGFHKN
                     GKSPLLLYAYGSYGNSLDASFSSTRLSLLNRGFAYAIAHIRGGQEMGRQWYEDGKMFK
                     KKNTFTDYIDCAEFLVKENFTSKEHLYAQGGSAGGLLMGAVINMAPNLWNGVIAQVPF
                     VDVVNTMLDESIPLTTNEFDEWGNPKKKEDYFYMKSYSPYENIEKKDYPNLLVTTGLH
                     DSQVQYFEPAKWVAKLRAMKTDKNVLLLHTNMEFGHGGASGRFDYLKDIALNYAFILA
                     LEGINQ"
BASE COUNT          975 a          463 c          403 g         1129 t
ORIGIN      
        1 gtatggcaaa ccaatttttc tgcttgatga aataagtgtg gtattgctcg ttttggtttt
       61 ccgctaaatg gtgtacaaaa aattgttagt gctttttcgg ctgctaattt tttagataaa
      121 agtgaaatgg ttttaatttt ggctctataa tagcttattg ctattctttg cgataacttt
      181 aacttcataa aaattcaaat atacgatatg aatgtggtaa tgattggttc aggaaaagta
      241 gcagctttac tttcaaaaaa aatacacaat gctgggctga aggtattgca agtatatagc
      301 ccaaacttgc aacatgcaac actattagct gatattataa atgctgaagc tactaacaac
      361 atagagaata ttatacaaag tgcaaacatt tatataattg caattgcaga tgacagtatt
      421 gaaggagttg caagcaaatt gaatgtttca aagaaagcat tggtattaca cacatctggg
      481 gctgtaaata tgaatgtatt gaaaagttgt agtgattttt atggggtatt atatcctatt
      541 caaagcattt ccaaaactac cgataataat gcagttttgc cttttgctat agatgccaat
      601 aatgatattg caatggagac aataaaattt attacaaatg cacttcattc taatgttgaa
      661 atattgaacg acttgcaacg tttgcactta catattgcag ctgtatttgt aaacaatttt
      721 gtaaactttt tatactttaa tgcaagtaat atttgtaaag acgctacaat taattttaat
      781 gttttacaac cattaataga agaaactgca ttaaggttga gaaacaattt acctgaagta
      841 gtatttacag gccctgcaat cagaaaagat tttagtacaa ttaataagca tttaaaagtt
      901 ttagaaacaa atgagcaact atttgaatta tattattttt taactaagtg cattttagtt
      961 aaagaaagct agaaaatttt agaatgaaat ttttataaat agaaaataat aaagaaaatc
     1021 ctttagataa tttactaaag gatttttaat tagttaattt tcattaagtt ttttgattat
     1081 tggtttatac cttctagtgc tagtataaag gcataattta aagcaatgtc ttttaaatag
     1141 tcaaaacgtc cacttgctcc gccatgtcca aattccatat tagtatgcag taaaagaaca
     1201 tttttatctg ttttcattgc tcttaattta gcaacccatt tagcaggctc aaaatattga
     1261 acttggctat cgtgtaagcc tgttgtaact aataaattag gatagtcttt cttttcaata
     1321 ttttcataag ggctataact tttcatataa aaataatcct cctttttttt agggttgccc
     1381 cattcatcaa actcgtttgt agttaaaggt atggattcgt cgagcattgt atttacaaca
     1441 tctacaaacg gaacctgtgc aattactcca ttccataaat taggagccat attaataact
     1501 gcacccatta ataaacctcc tgcactacca ccttgtgcat acaaatgttc tttgcttgta
     1561 aaattttctt taactaaaaa ttctgcacaa tcaatataat cggtaaaagt atttttcttt
     1621 ttaaacattt taccatcttc ataccattgc cttcccattt cttgtcctcc cctaatatgt
     1681 gcaatggcat aagcaaaacc tctgtttaat aaacttaatc tggtactgct aaaactagcg
     1741 tctaaactgt ttccataact accgtaagca tataataata aaggcgattt tccattttta
     1801 tgaaatcctt ttttatatac aattgaaata ggaattttag ttccatcttt agcagttgca
     1861 aataatcttt cagaaacata atctttagaa ttgtatccac ccaaaacttc ttgttgcttt
     1921 aatagagttt tgttcttact attcatatca taatcaaaaa cagagttggg cgtagttaaa
     1981 gaggtgaaat tgtaccttaa tttatctgta ttgtattctt cgttactgcc tatatatgct
     2041 gcataagtag gttctccaaa atctaaataa tgttctttat tttcttttgt gttttttata
     2101 tttatctgtg ttaatccatt ttttctttca gataaaacaa taaagttttt aaatacatct
     2161 acatcttgta acaaaatatc aggtctgtgt ggcaccaatt ctttccaatg ttctattcct
     2221 gttttatcta atgggcattc cattaattta aaattaatag cattgttatt agtaagaatt
     2281 aaaaatttat cctctaatgc ataaatggag tataaaacat tttttattct tggctgaaag
     2341 gatttaaaaa catcattagg ctttgctgca tctatataaa atatttcaga tgataatgtg
     2401 ccaccactgt aaatataaat atacttatcg ttctttgttt ttgaaattcc tatgtagttt
     2461 gaattatctt tttcatgata cactacaaca tcttttgcag catctgtacc taaaacatgt
     2521 tttttaattt tttcagataa tagcgttact gggtgttttg ctgtataaaa aatagtagta
     2581 ttgtcttctg cccaacatgc agatccttct gtatttggaa taacatcttt aagcaattca
     2641 cctgtttcta agttcttaat ataaatggtg tattgtcttc tacttacttc atctactcca
     2701 aatgctaaaa gtttattatt actgctaacc gaaaagccat tcacagaaaa atatgcttta
     2761 ccttctgcca ttttatctac atctaataat atttcttctg gtgcatctaa gctacctttt
     2821 tttctacaat acttaaagta ttgttttcca tcctctgttt tgctataata ataataacca
     2881 tttttaaatg taggtaccga ttcatctttt tcttttattc tacttttcat ttcagtaaat
     2941 aaatcagctt gtagtttttt agtgccatgt
//
LOCUS       DAOQPK010000256         2964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_25426_length_2964_cov_4.621176, whole genome shotgun sequence.
ACCESSION   DAOQPK010000256 DAOQPK010000000
VERSION     DAOQPK010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25426_length_2964_cov_4.621176"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2835)
                     /locus_tag="PLU36_09820"
     CDS             complement(<1..2835)
                     /locus_tag="PLU36_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HMZ47089.1"
                     /translation="MKQFIEGVAQKFKQFKPTSWAIDNRTAVYIITILISLYGITKFN
                     TLPKEQFPDIVVPTISINTIYFGNSPNDMENLVTRPIEKQLKGISGAKVKKIESISQQ
                     DFSLIVVEFDTDVKTEIAKQKVKDAVDKAQTDLPTDLTTQPNVQDFDFSEMPIMYVNV
                     SGDYDGMTLKEYADKMQDKFEELSEINRADIVGAPEREIQINVDQYKMQAAKISFTDI
                     SNAISFENRDISGGLVEVGEMKRSIRVKGQFANALEMQNLIVKNLQGAAVYLKDIAQI
                     VDTVKETESYARLDGKNVVTLNIIKRSGENLIEAATKVKAVVAEMQETGQLPPKDKLK
                     IVITGDQSKQTATSFHELINTIIIGFVLVLLVLMFFMGVNNAFFVALSVPLSIFVAFL
                     FLPVADFIVGTSVTLNFIVLFALLFGLGIIVDDAIVVIENTHRIYANGKVKIERAAKE
                     AAGEVFIPVLAGTLTTLAPFFPLLMWKGIIGKFMIYLPVMLILTLAASLIVAFIINPV
                     FAVSFMKPEGRQYEKPKNQIFKANWFWLFLSFGIILNLASWHGLGNFLIFIPLLAVLE
                     RYVLRDIIHFFQERLLPALMNKYEQLLKWILKGRRPAIALVSLFILFPISLFLLIARG
                     NKSTFFPSGDPNFIYVYLKMPVGTKTSTTDSVTAVLEKRVHKILANEMPEKNGIVESI
                     ITNVAVSANNPRDNNRSTQSNLGRIQVSFVEYEKRHGKTTMPFMEEIRKQMVGIPGAI
                     INVAQEDAGPPTDPPVNIEISGDDFQEISSVASALLFHLDSNRIEGIEGLKLDVDLLN
                     PEISIVVDREKALHEGLSTGQVGMEIRNAVFGKEASKLKDGEDDYKIQIRYADLQRNN
                     ITDIMNMRITFRDMNTMGIKQIPLSAIAKVDYTNTTGAVKRKNTKRTIQIQSNVTDPS
                     LAGPVNIELTKKINDFKSKTNIPGDVTIKQT"
BASE COUNT          913 a          510 c          414 g         1127 t
ORIGIN      
        1 tgtttgctta atggtaacat cgccaggaat atttgtttta gatttaaagt cgtttatttt
       61 tttagttaat tcaatattta caggcccagc taaagaaggg tcagtaacat ttgattgaat
      121 ttgaatagta cgtttagtgt ttttgcgttt tacagcacct gtggtattgg tgtaatctac
      181 ttttgcaatt gcactcagag gtatttgttt aatacccatt gtattcatat ctctaaatgt
      241 aatacgcatg ttcataatat ctgtaatgtt attacgttgt aaatcggcat aacgaatttg
      301 aattttataa tcatcttcac catcttttaa tttacttgct tctttaccaa aaacagcgtt
      361 cctaatttcc atacctactt gccctgtgct taatccttca tgtaatgctt tttctctatc
      421 taccactata gaaatttcgg ggtttaataa atctacatct agtttcaaac cttcaatacc
      481 ttcaattcta ttactgtcta agtgaaacaa tagggcagat gctacagatg aaatttcttg
      541 aaaatcatcg ccacttattt ctatgtttac tggagggtct gtaggcggtc ctgcatcttc
      601 ttgtgcaaca ttaattattg caccaggaat accaaccatt tgttttctaa tttcctccat
      661 aaatggcatt gtggttttac catgcctctt ttcgtattca acaaagctta cttgtattct
      721 tcctaaatta ctttgcgtac ttctgttatt atctcttgga ttattggcac taactgcaac
      781 attggtaatt atactttcta cgatgccatt tttttcgggc atttcattgg ctaaaatttt
      841 atgcactctt ttttctagaa ctgctgtaac agaatcggta gtggaggttt tagtgcccac
      901 tggcatttta agataaacat aaataaagtt aggatctccg ctaggaaaga aagtactttt
      961 attacctctt gcaattaata agaataatga aattggaaat aaaataaata aacttactaa
     1021 tgctatggct ggccttcttc cttttaaaat ccattttaat aattgttcat atttattcat
     1081 taatgcaggc aataatcttt cttgaaaaaa gtgaataata tctcttaaaa catatctttc
     1141 cagtactgct aataaaggaa taaatattaa gaaattgcct aacccatgcc agcttgctaa
     1201 atttagtata ataccaaaac ttaaaaatag ccaaaaccaa tttgctttaa atatttggtt
     1261 tttgggtttt tcgtattgcc taccttctgg cttcataaaa ctaactgcaa aaacagggtt
     1321 tatgataaaa gcaactatta acgatgcagc taaagttaga ataagcatta caggtaaata
     1381 aatcataaac ttgcctataa ttcctttcca cattaataaa ggaaagaatg gggctaaggt
     1441 ggttagtgta cctgctaata ctggaataaa aacttcgcca gcagcttctt tggctgctct
     1501 ttctattttt acttttccgt ttgcataaat acgatgggtg ttttctatca caacaattgc
     1561 atcatctaca ataattccta aaccaaatag caatgcaaat agcacaataa agtttagagt
     1621 aacgcttgta ccaacaataa aatctgcaac aggtaaaaat aagaaagcta caaatatgga
     1681 aagtggtaca cttaatgcca caaagaatgc attatttaca cccatgaaaa acattaacac
     1741 caaaagcact aaaacaaacc caataataat tgtattaata agttcatgaa aagaagtggc
     1801 agtttgttta ctttggtctc ctgtaattac aatttttaat ttatcttttg gtggtagttg
     1861 tcctgtttct tgcatctctg caactactgc ttttacttta gttgctgctt caattaaatt
     1921 ttcgccacta cgtttaatga tatttagtgt tactacattt ttaccatcta aacgggcata
     1981 gctttctgtt tcttttacag tatctacaat ttgtgcaatg tcttttaaat aaactgctgc
     2041 accttgtaag tttttaacta ttaagttttg catttctaaa gcattagcaa attgcccttt
     2101 aactctaatg cttcttttca tttctccaac ttctactaat cctccactaa tatctctgtt
     2161 ttcaaaactt attgcattgc taatatcggt aaaagatatt tttgcagctt gcattttgta
     2221 ttggtcaaca ttaatttgaa tttctctttc tggtgcacca acaatatcgg cacgatttat
     2281 ttcggaaagt tcttcaaatt tatcttgcat tttatcggca tattctttta atgtcatacc
     2341 atcataatct ccacttacat ttacatacat gataggcatt tcactaaaat caaagtcctg
     2401 tacattgggt tgtgtagtaa ggtctgtagg taaatctgtt tgagctttat ctacagcatc
     2461 tttaaccttt tgttttgcta tttcagtttt tacatctgta tcaaactcca ccacaattaa
     2521 actaaagtct tgttgcgata tgctttctat tttttttact ttggctccac ttataccttt
     2581 taattgtttt tcaattggtc tggtaaccaa attttccata tcatttggag agtttccaaa
     2641 ataaatggta ttaatactta tggtaggtac aacaatatca ggaaactgtt ctttaggtaa
     2701 agtattaaat ttagttatac catataatga aataagtatg gtaattatat atactgctgt
     2761 tctattatct attgcccagc ttgttggttt aaattgttta aacttttggg ctactccttc
     2821 aataaattgc ttcatacaaa atgttttact acattagttt taaaaataaa actaagcggt
     2881 aggtttttat ttaatgtaga ataatgttta gtaaacaaat tctaaaattg ttttttgggt
     2941 tctcttaaat ttatttttta tcca
//
LOCUS       DAOQPK010000257         2943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_25750_length_2943_cov_4.919668, whole genome shotgun sequence.
ACCESSION   DAOQPK010000257 DAOQPK010000000
VERSION     DAOQPK010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25750_length_2943_cov_4.919668"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PLU36_09825"
     CDS             complement(<1..617)
                     /locus_tag="PLU36_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HMZ47090.1"
                     /translation="MNLNNYTIKAQETIQAAQQLAFNNSNPNIETNHLLKAMLKDEDS
                     PIEFLLKKNDVNVVFVEKKLDESIKKLPKNSNGQTPENIGRDLNNALLKAVSSLKQFN
                     DEFVSVEHLLIGLLSVNDEVSKTLKDAGLTEKGLIAAIKDLRKGSTVNSQTQNQQYNA
                     LQKYSKNLNDLASNGKLDPVIGRDEEIRRTLHILTRRSKNNPILVG"
     gene            869..1915
                     /locus_tag="PLU36_09830"
     CDS             869..1915
                     /locus_tag="PLU36_09830"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HMZ47091.1"
                     /translation="MLVRLVMLCFLFSLTNFSKAQFNADAVNPVLMQNKKQLGNEAVL
                     MVYKDGKVIYKKKTDDYYDERAQTPIGSASSWLTAVLIMQYIENGTISLDTKIADYIP
                     IYIPYLKKYITIGHCLAQLTGIDFKKSASSSKEASKFNSLEEMVNSYAKKEIRANTGS
                     DFWYAEMHINIAARVIEVITKKKFEQLISKNVIKPLGMKGTSFSPSGGGCPNPAAGAV
                     STANDFANFLSMIMNKGMFNGKQILKEETINTMLTVTYTPSMVTYMPKAAENFEYGLG
                     AWILEKDINNKPAVFGTTNFEGIWCVIDKCRNYGMILFTKKELSDARKDVFYTLKNVI
                     DEQLPPTNGTSCNN"
     gene            complement(1934..2497)
                     /locus_tag="PLU36_09835"
     CDS             complement(1934..2497)
                     /locus_tag="PLU36_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47092.1"
                     /translation="MHTIEPYYNWRHIYISEEDKRSPFYKRFYSEFEYTQTVYNYFIH
                     PQWDFFGSKTLYLKIIYVDYELNFAIIELIGEWNDAIENDIMELKREVIDKLMEEGIF
                     KFILIAENVLNFHSGDKEYYEEWFEQVTDSNGWIVALNMPDATQYDFKKRKLNYFIEL
                     QELENWRVYKPYHLFKKIEVELNNRLT"
     gene            complement(2577..>2943)
                     /locus_tag="PLU36_09840"
     CDS             complement(2577..>2943)
                     /locus_tag="PLU36_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HMZ47093.1"
                     /translation="QMVDKAGKGVILYMNQEGRGIGLINKLKAYNLQENGMDTVEANL
                     HLGFKMDERDYGVGAQILRHLNVTKLKLLSNNPRKRAGLAGYGLEIVEVLPIEVTPNT
                     HNEKYLTTKRDKMGHTILK"
BASE COUNT          984 a          438 c          405 g         1116 t
ORIGIN      
        1 ccaactaaaa ttggattatt ttttgaccta cgagttaaaa tatgtaatgt tcttcttatt
       61 tcttcatctc taccaattac aggatctaac ttaccattgc ttgctaaatc gtttaaattt
      121 ttagagtatt tttgtaacgc attatactgt tggttttgtg tttgagagtt tactgtactg
      181 cctttgcgta aatcttttat tgctgctatt agcccctttt ctgttaagcc tgcatctttt
      241 aatgttttac tcacctcatc atttacacta agcaaaccaa ttaacaaatg ttctacactt
      301 acaaattcat cgttaaattg ttttaacgag gatactgctt ttagtaaagc attgttcaaa
      361 tccctgccta tattttctgg cgtttgacca tttgaatttt tgggtaactt ctttatgctt
      421 tcatctaatt ttttttcaac aaaaacaacg tttacatcat tcttttttaa taaaaattct
      481 ataggactgt cttcatcctt cagcattgct tttaataaat ggttggtctc tatatttggg
      541 tttgaattat taaaggcaag ttgctgtgca gcctgaatag tttcttgtgc tttaatagta
      601 tagttattta aattcatatt ttttaataat ttattataga agtactacaa ataaaaaacc
      661 agtatagtaa ataagaaaat gtgtcagtag tttattatca atactgtctg aaagtcttac
      721 tcagtttgaa ttttatgaaa taattacagt ttatggttag cttgattatg gttataatat
      781 taatttaatt ttattaaaaa agtttgttta taaaataatt ctactcaaac aattcggatt
      841 tattaatatt gtttttaaat tttttattat gcttgttcgt ttagtaatgt tatgtttcct
      901 attttctcta actaattttt ctaaggcaca atttaatgca gatgctgtaa acccagtgtt
      961 aatgcaaaat aaaaaacaac ttggcaacga agctgtttta atggtttata aagatggtaa
     1021 agtaatttat aaaaagaaaa cggatgatta ttatgatgaa agagcacaaa ccccaattgg
     1081 tagtgcaagc tcttggctaa ctgctgtttt aataatgcag tatattgaaa atggcaccat
     1141 atcattagat actaaaattg ccgattatat tcctatttat ataccttatc taaaaaaata
     1201 catcactatt ggacattgct tagcacaatt aacaggaatt gattttaaaa aaagtgcaag
     1261 ctcttctaaa gaggcttcta aatttaatag tttagaagaa atggttaaca gctatgctaa
     1321 aaaagagatt agagcaaata caggatccga tttttggtat gcagaaatgc atataaatat
     1381 tgctgcaaga gtaattgaag ttattactaa gaaaaaattt gaacaactta tttctaaaaa
     1441 tgtgattaag ccattaggca tgaaaggaac tagcttttct ccatctggtg gtggctgccc
     1501 taaccctgct gcaggtgctg taagtacagc aaatgatttt gccaattttt taagtatgat
     1561 aatgaataag ggcatgttta atggtaaaca aatactaaaa gaagaaacta ttaataccat
     1621 gcttactgtt acttatacac caagtatggt tacctatatg cctaaagctg cagaaaattt
     1681 tgaatatgga ttaggtgcat ggatattgga aaaagatatc aataataagc ctgcagtttt
     1741 tggtactact aattttgaag gtatatggtg tgtaatagat aaatgtagaa attatggtat
     1801 gatattattt acaaaaaaag aattatctga tgctagaaaa gatgtttttt ataccttaaa
     1861 aaatgtaatt gatgagcaat tgcctcctac aaatggtaca agctgtaata attaattaaa
     1921 aatatttttt gcattaagtt aatctgttat ttaattccac ctctattttt ttaaataaat
     1981 gatagggctt gtacacacgc caattttcta actcttgcaa ctcaataaaa taatttaatt
     2041 ttcttttttt aaaatcatat tgggttgcat ctggcatatt taaagctaca atccaaccat
     2101 tagaatctgt aacttgttca aaccattctt cgtaatattc tttgtctcca ctatgaaagt
     2161 ttaatacatt ttctgcaatt aaaataaatt taaaaatacc ctcttccatt agtttatcaa
     2221 ttacctcacg ttttaattcc ataatatcat tttctatagc atcattccat tcgccaatta
     2281 attcaattat ggcaaaattc aattcataat ctacataaat aattttaagg tataaagttt
     2341 tgctaccaaa aaagtcccat tgtggatgta taaaataatt ataaacggtt tgggtatatt
     2401 caaactcgct gtaaaatctt ttataaaaag ggcttctttt atcttcttcc gaaatataaa
     2461 tatgcctcca attataatat ggttcaattg tatgcattgt tattgcaaaa ttgcatcaaa
     2521 taaatgatta gaaaaagctg gaaaattgtt gtacaaaact tactaaataa aacgagttat
     2581 tttaaaattg tatgacccat tttatctctt ttggttgtta aatacttttc gttatgtgta
     2641 tttggtgtaa cttctattgg taatacttct acaatctcta atccataacc tgcaaggcca
     2701 gctcttttac gtggattgtt gctcagtaac ttaagttttg ttacattaag atggcgtaaa
     2761 atttgagctc caacgccata gtctctttca tccattttaa accctaaatg taaattagct
     2821 tcaacggtat ccattccatt ttcttgaagg ttataggcct ttaatttatt tattaaacca
     2881 attcctcttc cttcttgatt catataaaga attactcctt tacctgcctt atctaccatt
     2941 tgc
//
LOCUS       DAOQPK010000258         2936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_25850_length_2936_cov_4.274557, whole genome shotgun sequence.
ACCESSION   DAOQPK010000258 DAOQPK010000000
VERSION     DAOQPK010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25850_length_2936_cov_4.274557"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PLU36_09845"
     CDS             <1..553
                     /locus_tag="PLU36_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HMZ47094.1"
                     /translation="VQENNLETLFNHVEFTNNWKGKTEQLVLQIFYHTGMRLSELIQL
                     KTVNIDFYYQQIKVLGKGNKERIIPISKELLIDIQAYINLKPEALKQVPQLLVTENGT
                     ALQPRSVYAMVKKYLSMVTTVQKRSPHVLRHSFATHLMNSGADLNAVKELLGHSSLAA
                     TQVYTHNTIEQLKDIFKKAHPKA"
     gene            671..2830
                     /locus_tag="PLU36_09850"
     CDS             671..2830
                     /locus_tag="PLU36_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HMZ47095.1"
                     /translation="MKKIIVSLVFALTAFRSYADEGMWLPMLLGQQVYKDMVKKGLKL
                     KPEQLYSINKSSIKDAIIIFGGGCTGEIVSNQGLIFTNHHCGYGAIATASTVEANYLR
                     DGFWAADKSKEISTNLSVKFLNRIEDVTEKINAELKNLSGAERDKKYREVVAKIVKEI
                     VGTSEFKDAVVASMFKGNQFFVFVYDVYKDIRLVGTPQESIGKFGGDTDNWEWPRHTG
                     DFSVFRVYTNADGSPANYNANNIPLKPKYHLPVSIKGIKDGDYSMIFGYPGGTNRYET
                     SYGVKLATDINNPSLVALRAERLKYMYNQMKNDAAVKLQLASRYASIANYWKFFDGET
                     RQLLKFDTYGAKQKDEAAFIEWAKDKAEYKNIFSDYEANYNAWTPFAKGRQYLNEGIF
                     GSPLVAYVSSWASLERRLARKDYNPEEIKKAMDAADKARKKFLEDENKKSDQQILATV
                     VSMFYSNIDKSQHPKGFYEDLFNNYNDIEQNPYKMFAADVFENTMAFDDAKWKAFMEK
                     PSAINIVEDPAYKVAKAFISNYSDNYAPKYTDFVNKNNDLGRLYMKGLFEMNPTKMNG
                     TYPDANFTMRVSYGNVKSYKPKDAVTYDYYCTLAGTVAKYKKGDYEFDLPAKLLELYK
                     NKDFGDYADKKLGDIVVNFITTNDITGGNSGSPVIDAKGNLIGLAFDGNSEALSHKLA
                     FDKELNRTICVDVRYVLWCIEKVGGAKHIIDELTLVK"
BASE COUNT         1149 a          404 c          510 g          873 t
ORIGIN      
        1 tgttcaagaa aataatttag aaaccttatt taatcatgta gagtttacca ataattggaa
       61 aggtaaaaca gagcaattag tacttcaaat attttatcat acaggtatga ggctaagtga
      121 attaatacaa cttaaaacag taaatattga tttttattac caacaaatta aagtattagg
      181 caaaggcaat aaagagagaa ttatacctat atctaaagag cttttaatag atatacaagc
      241 atatattaat ttaaagccag aagctttaaa acaagttcca cagttattag taacagaaaa
      301 tggtacagca ttacaaccac gcagtgtata tgccatggta aaaaaatatt taagcatggt
      361 taccactgtt caaaaaagaa gccctcacgt attaaggcat agttttgcaa cccatttaat
      421 gaatagtggt gccgatttaa atgcagtaaa agagctactt gggcatagca gccttgcagc
      481 aacacaggtt tatacacata atacaataga acaattgaaa gatatattta aaaaagccca
      541 tccaaaggct taaattatat actaattcat ccaatttact taccatttat atattaatta
      601 aaaatttctt tttaaaaaca aatttttaaa aacacattat ttattacttt gccgcaataa
      661 aatttgcaat atgaaaaaaa taattgtttc tctagtgttt gctttaactg cttttcgtag
      721 ttatgcagat gaaggcatgt ggctaccaat gttattgggg caacaagttt ataaggatat
      781 ggttaaaaaa ggcttaaagc taaaaccaga acagctttat agtattaata aatcatctat
      841 aaaagatgct attattattt ttggtggagg ttgtacaggc gaaatagtaa gcaaccaagg
      901 cttaattttt accaaccacc attgtggtta tggtgcaatt gctactgcaa gtacagtaga
      961 ggcaaactac ttaagagatg gattttgggc agcagataaa agcaaagaaa tatccactaa
     1021 tttaagtgtt aagttcttaa acagaattga agatgtaaca gaaaaaatta atgcagaact
     1081 taaaaattta tcaggagccg aaagagataa aaagtataga gaagtagttg ctaaaattgt
     1141 aaaagaaata gttggcacaa gcgaatttaa agatgcagtt gtagctagta tgttcaaggg
     1201 aaatcagttt tttgtttttg tgtatgatgt gtataaagac attcgcctag taggtacacc
     1261 acaagaaagt attggaaaat ttggaggcga tacagataat tgggagtggc ctcgccatac
     1321 aggcgatttt tctgtttttc gtgtttatac aaacgcagat ggttcaccag caaactataa
     1381 tgctaacaac ataccattaa agccaaagta tcatttacct gtaagcatta aaggaattaa
     1441 ggatggtgat tatagcatga tttttggcta cccaggcggt acaaacagat atgaaacatc
     1501 ttatggtgtt aaacttgcaa cagatatcaa taatccatct ttagtagcat taagagcaga
     1561 aagattaaaa tacatgtata accaaatgaa aaatgatgct gccgtaaaat tacaattagc
     1621 cagccgttat gcaagtattg ccaactactg gaaatttttt gatggagaaa caagacaact
     1681 cttaaaattt gatacttatg gtgcaaaaca aaaagatgaa gctgcattta tagaatgggc
     1741 aaaagataaa gcagaatata aaaatatttt tagcgattat gaagctaact ataatgcatg
     1801 gacacctttt gctaaaggaa gacaatattt aaacgaagga atttttggaa gcccattagt
     1861 tgcttatgta tcaagttggg caagcttaga aagaagatta gcaagaaaag attataaccc
     1921 ggaagaaatt aaaaaagcta tggatgctgc tgataaagca agaaaaaaat tcttagaaga
     1981 tgaaaacaaa aaaagcgatc agcaaatatt agcaaccgta gtttctatgt tttatagcaa
     2041 tatagataaa agccaacatc caaaaggttt ttacgaagat ttgtttaata attacaacga
     2101 tattgagcaa aacccttata aaatgtttgc agcagatgtg tttgaaaaca caatggcttt
     2161 tgatgatgca aaatggaaag catttatgga aaaaccaagt gcaattaata ttgtagaaga
     2221 ccctgcttac aaagtagcaa aagcctttat tagcaactat tcagataatt atgcacccaa
     2281 atacacagat tttgtaaaca agaacaatga tttaggaaga ttatacatga aaggtttatt
     2341 tgaaatgaac cctactaaaa tgaatggaac ttatccagat gcaaatttta ccatgcgtgt
     2401 ttcttatggt aatgtaaaaa gctataaacc aaaagatgca gtaacttacg attactattg
     2461 cactttagca ggtacagtag ctaaatataa aaaaggcgat tatgaatttg atttgcctgc
     2521 taaattactt gagctttata aaaacaaaga ttttggagac tatgccgata aaaaattagg
     2581 cgatattgtg gttaacttta ttactactaa tgatattaca ggtggtaact ctggaagccc
     2641 agtaatagac gcaaaaggca atttaattgg attggcattt gatggcaatt ccgaagcctt
     2701 aagccacaaa ttagcatttg ataaagaact aaaccgtaca atatgtgttg atgttcgtta
     2761 tgtattatgg tgtatagaaa aagtaggtgg agcaaaacac ataattgatg aacttacttt
     2821 agttaaataa aatacatttt aaagtaattg ttgctaaaac cctgaaacat ttcagggttt
     2881 ttttatatat atgtaatcat tttttcaaaa acttaacctc aaatattata gagtct
//
LOCUS       DAOQPK010000259         2924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_26066_length_2924_cov_4.834437, whole genome shotgun sequence.
ACCESSION   DAOQPK010000259 DAOQPK010000000
VERSION     DAOQPK010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26066_length_2924_cov_4.834437"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="PLU36_09855"
     CDS             <1..1100
                     /locus_tag="PLU36_09855"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ47096.1"
                     /translation="YFYGTIFSSEYKKNKTNLTIGGGWSKYDGKHFGKLPWAAIGIEK
                     DYQYYNNPATKTEFNIYSKLQQQVSHSITLFSELQCRNVQQLMNGFRNNPSLLVNRKF
                     DFVNPKIGLTFIKKGWQTYASYAMVGKEPNRDDFEAGTITQPKAEILHNIELGTEKKT
                     SKYNLAANIYVMLYKNQLVLTGKINDVGAYTRINVPKSYRVGIELQGSYVFSNWLSMM
                     ANITISKNKIQQFTEYIDDYDNGTQVQINQSNKTIALSPSFISSASINFLPLKNIEFS
                     LIGKFVGKQYLDNTESNLKQLNSFYTQDVRLIYSIKNCNLSFQVNNILNTLYQPNGYT
                     FNYIYGGKLIIENYYFPMAGINFLAGVNIKL"
     gene            1150..1743
                     /locus_tag="PLU36_09860"
     CDS             1150..1743
                     /locus_tag="PLU36_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ47097.1"
                     /translation="MNVKKIVVVGAESTGKSTLCTMLAQHYKTEWCAEFAREYLLKNG
                     TNYNFESLEIIAKGQLALEDECNNKVSKNWQPIADGKFINSHTLTTINHQPLLFIDTN
                     MYVMKVWCEFVFNKCHQFILDEIVSREYDLYLLCNIDLPWIADELREYPNEEPRQMLN
                     NIYKDILINQSTPWAEIKGSNYINRFKQAVKAINTIL"
     gene            complement(1750..2502)
                     /locus_tag="PLU36_09865"
     CDS             complement(1750..2502)
                     /locus_tag="PLU36_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47098.1"
                     /translation="MAINFIQAQYTTISGVVYDISAKRPIEAVAVISTSGKGTITDSL
                     GRYQITVKNTDSIWFSLIGKSTMKYVVDTISNPFAFDVMIHVHVAQLPEVKVRNKNYR
                     LDSIQNRKDYAYVFNYKKPSISLLQTPSYNSTPGVSVGIDLIEFINMFRFKRNRQILA
                     LQKRLEVQEREKYIDHRFKKLLITKLTGLQQPQLDTFMLNYRPEYYFLQRLNDLELGY
                     YIQQCYQAYKLNLPNPYKTQERNNNERDENLN"
     gene            2644..>2924
                     /locus_tag="PLU36_09870"
     CDS             2644..>2924
                     /locus_tag="PLU36_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HMZ47099.1"
                     /translation="MLFPRLTHTTDIAKLIDAEVLGKVDAFAKGINEIHKVNEGDICF
                     VDHPKYYSKCLNSAATFIIINTKDITIPAGKTIFFVKEPFEAYLKIVHH"
BASE COUNT         1101 a          379 c          431 g         1013 t
ORIGIN      
        1 attattttta tggaactatt ttttcatctg aatataaaaa aaataaaaca aatttaacta
       61 ttggaggggg ttggagtaaa tacgatggta agcattttgg caaactgcct tgggcagcca
      121 ttggtataga gaaggattat caatattata ataatcctgc aacaaaaaca gaatttaata
      181 tatattcaaa attacaacaa caagtatctc attctataac attattttct gaattgcaat
      241 gtagaaatgt tcaacaatta atgaatggat ttagaaataa tccttcttta ttggtaaata
      301 gaaaatttga ttttgttaat cctaaaatag gtttaacttt tattaaaaaa ggctggcaaa
      361 cttatgcaag ctatgctatg gtaggtaaag aacccaatag agatgatttt gaagctggaa
      421 caattacaca gccaaaggca gaaattttac ataatataga attgggaacg gaaaagaaga
      481 catctaaata taatttggct gcaaacatat atgtaatgtt atataaaaac caattagtgt
      541 taacaggtaa aataaatgat gttggagcct ataccagaat taatgtaccc aaaagttata
      601 gagttggtat agaattacaa ggaagctatg tattttcaaa ctggttaagt atgatggcaa
      661 atattaccat cagtaaaaat aaaatacaac aatttacaga gtatatagat gattatgata
      721 atggtactca ggttcaaatt aaccaatcta ataaaaccat tgctttgtca ccatctttta
      781 taagtagtgc cagtatcaat tttttacctc taaaaaatat tgaatttagt ttaattggta
      841 aatttgttgg taagcaatat ttagacaata cagaaagtaa tttaaaacaa ttaaacagct
      901 tttatactca agatgtaaga ttaatttact ctattaaaaa ttgcaacctt tcgtttcagg
      961 taaataatat tttaaacaca ttgtaccaac ccaatggcta tacttttaat tatatttatg
     1021 gtggaaaatt aattattgaa aactattatt ttccaatggc tggaataaat tttttagcag
     1081 gggtaaatat aaaattatag ttagttaaaa atagttatac ttgctacatt atagtaatgc
     1141 acaattagta tgaatgtaaa aaaaattgta gtggtaggtg ccgaaagtac tggaaaaagt
     1201 actttgtgta caatgttggc acaacactat aaaacagaat ggtgtgccga atttgcaaga
     1261 gaatatttat taaaaaatgg tacaaattat aattttgaaa gcctcgaaat tattgcaaaa
     1321 gggcagcttg ctttagaaga tgaatgcaac aacaaagtct ctaaaaactg gcaaccaata
     1381 gcagatggaa aatttataaa tagccatacc ttaactacta taaaccatca accattatta
     1441 tttatagata ccaatatgta tgtaatgaaa gtgtggtgtg aatttgtttt taataaatgt
     1501 catcaattta ttttagatga aattgtaagc agagaatatg atttgtactt gctttgcaat
     1561 attgatttac cttggattgc cgatgaatta agagaatatc ctaatgaaga acctaggcaa
     1621 atgctgaata atatttataa agatattctt attaaccaat caaccccttg ggcagaaata
     1681 aaaggtagta attatattaa cagatttaaa caagcagtta aagcaattaa tacaattctt
     1741 taaaaaaaat taattcaaat tttcatctct ctcgttatta tttctttctt gtgttttata
     1801 cggattaggc aggtttaatt tgtatgcctg ataacattgt tgtatataat agcccagttc
     1861 taaatcattt aacctttgta aaaaataata ttcaggtcta tagtttaaca taaaagtatc
     1921 taactgtggt tgctgcaaac cagttagttt agttataagt agtttcttaa aacgatggtc
     1981 aatatatttt tctctttctt gaacttctaa tcttttttgc aatgccagta tttgcctgtt
     2041 tcttttaaac ctaaacatat taataaactc tattaaatca ataccaacac ttacaccagg
     2101 ggttgaatta taagaaggag tttgtaataa gctaatactt ggttttttat aattgaaaac
     2161 gtaagcataa tcttttctgt tttgaataga atctaacctg taattcttgt ttctaacttt
     2221 aacttctggc aattgagcta catgaacatg tatcataaca tcaaatgcaa aagggttact
     2281 aatagtatcc acaacatact tcatggttga tttaccaatt aaagaaaacc aaatagagtc
     2341 ggtgtttttt actgtaattt gataccttcc taaagagtct gtaatagtac cttttcctga
     2401 agtactaatt acagcaactg cttcaattgg gcgttttgca gaaatatcat acactacacc
     2461 actaatggta gtgtattgtg cttgtataaa attaattgcc aataaacaga ctaataaagt
     2521 aaatagcttt ttaataaaca aaatattttt gtaatattaa taaagcaaca ctgattatag
     2581 cactataatt tattttatta actgtttttt gtagattata tacctttgcc atcaaatttt
     2641 tatatgcttt ttcctcgtct aactcataca acagatattg caaaacttat tgatgctgaa
     2701 gttttaggaa aagtggatgc ttttgcaaaa ggaattaatg aaattcataa ggtgaatgaa
     2761 ggagatattt gttttgtgga tcatccaaaa tattattcaa aatgtttaaa tagtgctgct
     2821 acatttataa taattaatac aaaagatata actatacctg cgggcaaaac tatttttttt
     2881 gtaaaggaac cttttgaggc atacttaaaa attgtacatc attt
//
LOCUS       DAOQPK010000260         2908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_26324_length_2908_cov_5.077112, whole genome shotgun sequence.
ACCESSION   DAOQPK010000260 DAOQPK010000000
VERSION     DAOQPK010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26324_length_2908_cov_5.077112"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="PLU36_09875"
     CDS             complement(<1..735)
                     /locus_tag="PLU36_09875"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ47100.1"
                     /translation="MQSNNNSFSLRFLKNKAAVFALLFIFLVLIIAIFAYLIAPENSS
                     NANRIIPEIGSKKPGFSIYLLKTKQRESAQENFFTIVLNGKSDNFFYTPITEYKILKD
                     SLYYQKFIDEDITENKVIKIQGSNFEENNIIKQTFYLGTDKFGRDILSRLIIGSRVSL
                     GVGCIAAIISLTIGVFLGAVAGYFKGKTDNVIMLLINIVWSIPTLLLVFAITLVLGKG
                     FWQVFIAVGLTMWVNVARLVRGQVMIA"
     gene            complement(749..1861)
                     /locus_tag="PLU36_09880"
     CDS             complement(749..1861)
                     /locus_tag="PLU36_09880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ47101.1"
                     /translation="MAYAIVLLFYRKWFLQLKHFNLPQNYTPTTSFSIIIPARNEEAN
                     IRTCLTSILQQQYPTTLFEIIVIDDHSSDNTSLIVEELQQQYSNLKLIKLVDELDGEM
                     LNSYKKKAIENAISYAKANWIITTDADCFMQTKWLSIFDAWIQENDTVFVAAPVAFTN
                     NGSLLQTFQYIDFMSLQGITAASVNAGFHNMCNGANLAYKKEVFFEVNGFKGIDNIAS
                     GDDMLLMNKIKNHYTNGVSFLFSKQAIVTTHPMPTWSSFFNQRIRWASKAEQFKTKDT
                     SIFIVLVLVYLLNVTLFIMPFLSIFNFIFLWYWLLFIMLKTIAELAFALPVAKFFSQP
                     FIWWFPFLQPLHIVYIVVSGWLGKFGTYNWKGRNVK"
     gene            2023..>2908
                     /locus_tag="PLU36_09885"
     CDS             2023..>2908
                     /locus_tag="PLU36_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47102.1"
                     /translation="MHTAIKKYIITFIFATVIAHSLFAQSCKINYVITDTINNKTLQS
                     FKLLQQFNDSIACINYINQLPNNLQAKGYISASVDSIFIYKNNYTVTIFVGKKFNWKL
                     LKISDTDLISLQLAGIDHTIFSAKPFNPKQVELVQNNLLEYFVNNGYPFASIGFSNLI
                     IEENDITATLRINKGIFYSMDSISIIGNAKISKQFLYKYLNLPKKSLFSLKKLQSIDK
                     KIAELNFINSYHPSQITMLGASYYTNLFLNNKKSNQVDAIIGLLPNNTQNNGSLLLTV
                     DAKLKLENAFAKGETIGVL"
BASE COUNT         1085 a          450 c          371 g         1002 t
ORIGIN      
        1 tgcaatcatt acctggccac gaactaatct ggcaacattt acccacatgg ttaatccaac
       61 tgcaataaaa acttgccaaa aacctttgcc caatacaagt gtaatggcaa acactaaaag
      121 taaagtagga atactccaaa caatatttat taatagcata attacattgt cggtttttcc
      181 tttaaaataa cctgcaacag cacctaaaaa aacgcctata gttaatgaaa taattgcagc
      241 aatacaacct acgcctaaac ttactcgaga gcctattatc aatctgctta aaatatctct
      301 tccaaactta tcggttccta aataaaatgt ttgttttata atattgttct cttcaaaatt
      361 gctaccttgt atttttatta ctttattttc tgtaatgtct tcatcaataa atttttgata
      421 gtataaacta tcctttaata ttttatattc ggttatggga gtataaaaaa agttatctga
      481 tttgccgttg agcactattg taaaaaaatt ttcttgtgca gattctctct gttttgtttt
      541 taataaataa atggaaaagc ctggtttttt gctaccaatt tctggaataa ttctattggc
      601 attgctgcta ttttctggtg caattaaata ggcaaatatt gcaattatta atactaaaaa
      661 aataaaaagt aatgcaaaaa ctgctgcttt attttttaaa aaccttaagc taaaactatt
      721 gttgttactt tgcattatag aaactttact attttacatt tcttcctttc caattataag
      781 taccaaattt tcctaaccat ccacttacca caatataaac tatatgtaag ggttgtaaaa
      841 atggaaacca ccaaataaat ggctgagaaa aaaactttgc aacaggcaat gcaaaagcaa
      901 gttctgcaat agttttaagc attataaaca gcagccaata ccataaaaaa ataaaattaa
      961 aaatacttaa aaaaggcatt ataaataatg taacattcaa taaataaacc aataccaaca
     1021 ctataaaaat actggtatct tttgttttaa attgctctgc tttacttgcc catcttattc
     1081 tttgattaaa aaaagaactc caagttggca tagggtgtgt ggtaacaatt gcttgtttag
     1141 aaaataaaaa gcttactcca ttagtgtaat ggtttttaat tttattcatt aaaagcatat
     1201 catctccgct ggcaatatta tcaattcctt taaatccatt tacttcaaaa aaaacctctt
     1261 ttttatacgc caaattagcc ccattacaca tattatgaaa gcctgcattt acagatgcag
     1321 cagttatacc ctgtaggctc ataaaatcta tatattgaaa agtttgcaac aaggaaccat
     1381 tgttagtaaa agcaacagga gctgcaacaa aaacagtatc attttcttga atccatgcat
     1441 caaagattga taaccatttt gtttgcatga agcagtcagc atctgtagtt attatccagt
     1501 ttgcttttgc ataactaatt gcattctcta ttgctttctt tttgtaagag ttcaacattt
     1561 ccccatctag ctcatctact aactttatca gtttaagatt tgaatactgc tgctgtaact
     1621 cttctacaat taaagaagta ttatcagagc tatggtcatc tatcacaata atttcaaaca
     1681 aagttgtagg gtactgttgc tgtagtatag atgttaaaca agttcttatg ttagcttctt
     1741 catttctcgc aggaattata atactaaagc ttgtagtggg agtataattt tgggggagat
     1801 taaaatgttt taattgtaag aaccattttc tataaaacaa caaaacaatg gcgtaagcca
     1861 caaaacacaa aaaagtaaaa attaaaaaca agttaagcca catgcataca aagaaacaat
     1921 atagtacaat ataatgcttt gtttagccaa tattaaatga aatttaaaca aattgcgtcc
     1981 ttcatactct gtaatatttt agctttacaa ctgttaatac tgatgcatac tgctataaaa
     2041 aaatatatta tcacctttat ttttgctaca gttatagctc attccttatt tgcacaaagc
     2101 tgtaaaataa attatgttat aacagataca attaacaata aaactttgca gagctttaaa
     2161 cttttgcagc agtttaatga tagcatagct tgtattaatt atatcaatca attacctaat
     2221 aacctgcaag caaaaggata tattagtgca tcggtagata gtatttttat ttataaaaac
     2281 aactatacag taacaatttt tgttggcaaa aaattcaatt ggaaattatt gaaaatttct
     2341 gatactgatt taatttcttt acaattagca ggtatagacc atactatttt ttcggctaaa
     2401 ccatttaatc caaagcaggt tgaattagtt caaaataatt tgttagaata ttttgtaaac
     2461 aatggctacc cttttgccag tataggtttt agtaatttaa taatagaaga aaatgatata
     2521 actgcaactt taagaataaa taaaggtata ttttattcaa tggatagtat tagcataatt
     2581 ggtaatgcta aaataagtaa gcagttttta tacaaatatt taaaccttcc taaaaaaagt
     2641 ttatttagtt taaaaaaatt acaaagtatt gataaaaaaa ttgcagaatt aaattttatt
     2701 aattcttatc acccaagcca aataaccatg cttggagcaa gttattatac aaatctgttt
     2761 ttaaataata agaaaagtaa tcaggtagat gcaattattg gcttattacc aaacaatact
     2821 caaaacaatg gttctttact tttaactgta gatgcaaaat taaaattaga aaatgctttt
     2881 gcaaaaggtg aaactattgg agttttgt
//
LOCUS       DAOQPK010000261         2892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_26599_length_2892_cov_4.190342, whole genome shotgun sequence.
ACCESSION   DAOQPK010000261 DAOQPK010000000
VERSION     DAOQPK010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26599_length_2892_cov_4.190342"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..2706
                     /gene="cphA"
                     /locus_tag="PLU36_09890"
     CDS             85..2706
                     /gene="cphA"
                     /locus_tag="PLU36_09890"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HMZ47103.1"
                     /translation="MRIEEIKVLKGPNFWSIRRNKLIQMKLNLEELEQKPTNKIDGFS
                     ERLEAMFPSMYSHRCSEAKPGGFFSRVKEGTWMGHVIEHIALELQTLAGMDTGFGRTR
                     GTGEEGKYYVVFSYMEEDAGTYAAKAAVKIAQALTDGTEYDLANDIQRLREIREDTRL
                     GPSTGCIVDEAAKRGIPHLRLNKHSLVQLGYGVHQKRIRATIASTTSNIAVDIACDKE
                     ETKLLLEAAEIPVPKGMVIRTEVGLKEAVDKYGYPLVIKPIDGNHGKGNTTNITTWEQ
                     ALKALENAKQYGRSVIVERFITGFDFRILVINYKFICAALRTPAAVIGDGTHNIQWLI
                     DETNKDPRRGYGHEKVLTQITVDHSTMKMLEEKGYTLETIPVKDELVLLKTTANLSTG
                     GTSTDVTDEVHPDNIVMCERIAKIVGLDICGIDIMAKDLHTPVTENGGAILEVNAAPG
                     FRMHIEPAIGLPRNVAEPVVEMLFPDRSNGRIPIIAVTGTNGKTTTTRLTAHISKSAG
                     KKVGYTTSDGVYIQNQLLMKGDCTGPISSQFVLKDPTVDFAILECARGGILKSGLAFQ
                     NCDVAIVTNVAEDHIGLGGINNLEQMAKVKAVVPETVFKHGYAVLNADDDLVFNMIPT
                     LSCNIALFSMNENNPRIVEHCNAGGLATVYENGFISILKGTWKIRVAHVKDIPITFEG
                     KAVHNIANCLPSVMATYLFKSIKIEDIRSGLQTFIAGSNTTPGRLNFFNFKNFTFLAD
                     FAHNPHGLKLLGQFVSKLDYKTKVGVISGTGDRRDEDIRELGAISAEYFDEIIIRCDK
                     NLRGRTAEEIIGLLQEGINTVNPNIPQTVIANENLALEHIYANHKPGALYTIMCDVVA
                     GALDKIKELKDRENKNG"
BASE COUNT         1067 a          408 c          565 g          852 t
ORIGIN      
        1 agtttggatc tccgaacaaa ggcctggttg actctataaa atatatttta tttatcatca
       61 aacatttcaa ccaataatta gtgtatgaga attgaagaaa ttaaagtttt aaaaggacca
      121 aatttttgga gtattcgtcg taataagctg atacagatga agcttaatct ggaagaatta
      181 gaacaaaaac caaccaataa aatagatggt tttagcgaaa gattagaagc tatgtttcct
      241 agtatgtata gtcatagatg cagcgaagct aagccaggtg gtttttttag cagagtaaaa
      301 gaaggtactt ggatggggca tgtaattgag catattgcat tagagctaca aaccctagca
      361 ggcatggata caggctttgg cagaacacgt ggcaccggcg aagaaggaaa atattatgtt
      421 gtatttagtt atatggaaga agatgcaggc acttatgctg caaaggctgc agttaaaatt
      481 gcacaagctt taaccgatgg tacagaatat gatttagcta acgatattca acgattaaga
      541 gaaattagag aagataccag acttggacca tccacaggtt gtattgtaga tgaagcagca
      601 aaacgtggta ttccgcattt acgtttaaac aagcacagtt tggtacaatt gggttatggt
      661 gttcatcaaa aaagaataag agcaactatt gcaagtacaa cctctaacat agctgtagat
      721 attgcctgcg ataaagaaga aaccaagctt ttattagaag ctgcagaaat tccggttcct
      781 aaaggaatgg ttatcagaac agaagtggga ttaaaagaag ccgtagataa atatggttat
      841 ccgttagtga taaaaccaat tgatggaaac catggcaaag gaaacaccac aaatataacc
      901 acctgggaac aagcactaaa agctttagaa aatgcaaaac aatatggtag aagcgttatt
      961 gtagaacgat ttattaccgg tttcgatttt agaattttag ttattaatta taaatttatt
     1021 tgtgcagcat taagaacacc tgctgcagtt attggagatg gaactcataa tattcaatgg
     1081 ttaatagacg aaaccaataa agacccaaga agaggttatg gtcacgaaaa agtattaaca
     1141 caaattaccg tagatcatag caccatgaaa atgctggagg aaaaaggata taccttagaa
     1201 actattcctg ttaaagatga attagtttta cttaaaacaa ctgctaacct tagcacaggt
     1261 ggtacaagta cagatgttac agacgaggtg cacccagata atattgtaat gtgtgaacgt
     1321 attgcaaaaa ttgttggttt ggatatttgt ggtattgata ttatggcaaa agacttacat
     1381 acacctgtta cagaaaatgg aggagccatt ttagaagtta atgctgctcc gggttttaga
     1441 atgcatattg aacctgccat tggtttacct cgtaatgttg cagaacctgt tgttgaaatg
     1501 ttgtttccag atagaagcaa tggcagaata cccattattg cagttacagg tacaaatggt
     1561 aaaactacaa ctacccgttt aactgctcat attagcaaaa gtgcaggcaa aaaagttggt
     1621 tatacaacga gcgatggagt gtatatccaa aatcaattat taatgaaagg agattgtaca
     1681 ggcccaatat cttctcaatt tgttttaaaa gatcctactg tagattttgc cattttagaa
     1741 tgtgcaagag gtggtattct taaaagtggt ttagcatttc aaaattgcga tgttgcaata
     1801 gttactaatg tggctgaaga tcatattggc cttggcggaa taaataattt agaacaaatg
     1861 gctaaagtaa aagctgttgt tccggaaaca gtttttaaac atggatatgc tgtattaaat
     1921 gcagacgatg atttagtatt taatatgatt cctactctct cttgcaatat tgccttattt
     1981 agcatgaatg aaaataatcc aagaattgtg gaacattgca atgctggagg cttagcaaca
     2041 gtttacgaaa atggctttat aagtatttta aaaggtacat ggaaaataag agttgcacat
     2101 gtaaaagata ttcctataac atttgaagga aaagcagttc ataatattgc caactgctta
     2161 ccaagtgtta tggcaactta tttattcaaa tcaattaaaa ttgaagatat aagaagcgga
     2221 ttacaaactt ttattgcagg tagtaataca actcctggac gattaaactt tttcaacttt
     2281 aaaaacttta catttctagc cgactttgct cataaccctc atgggttaaa gcttttaggg
     2341 caatttgtaa gtaagctaga ttataaaacc aaggtaggtg ttattagtgg aacaggtgac
     2401 agaagagacg aggatattag agaacttgga gcaatatctg ccgaatattt tgatgaaata
     2461 ataattagat gtgacaaaaa tttaagaggt agaacagccg aagaaataat tggcctttta
     2521 caagaaggta ttaatacagt aaaccctaat attcctcaaa cagttattgc caacgaaaat
     2581 ttagcattag aacatatata tgccaaccat aaacctggtg cactttatac aataatgtgc
     2641 gatgtagtgg ctggtgcttt agataaaata aaagaactaa aagacagaga aaataaaaat
     2701 gggtgataat taatttatta ctgcatttat aattactatt tccattattt aagtacaaaa
     2761 atgcttaaaa aatatagttt actaaccact ttattattgg ttatatttat tggtacatct
     2821 tgtaaaaaaa taaaaagttt tgaatacaga gatgtaagaa atattgaagt aaaatcatta
     2881 gggtttaaca ag
//
LOCUS       DAOQPK010000262         2872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_26917_length_2872_cov_4.117856, whole genome shotgun sequence.
ACCESSION   DAOQPK010000262 DAOQPK010000000
VERSION     DAOQPK010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26917_length_2872_cov_4.117856"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..599)
                     /gene="rpsG"
                     /locus_tag="PLU36_09895"
     CDS             complement(132..599)
                     /gene="rpsG"
                     /locus_tag="PLU36_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HMZ47104.1"
                     /translation="MRKAQAKKLPLAPDARFNDKQVTRFINNLMWDGKKSTAITIFND
                     AVDKVSSITGEDGYEVWKKALANVTPAVEVRSRRIGGATFQIPSEVRADRKISLSMKW
                     LIRYSRERNGKTMADKLASEIISASKGEGAAFKKKEDVHRMAEANKAFSHFKV"
     gene            complement(681..1061)
                     /gene="rpsL"
                     /locus_tag="PLU36_09900"
     CDS             complement(681..1061)
                     /gene="rpsL"
                     /locus_tag="PLU36_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HMZ47105.1"
                     /translation="MPTIQQLVRKGREIIRAKSKSRALDACPQRRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNKIEVIAYIPGEGHNLQEHSIVLIRGGRVKDLPGVRYHIVRGSL
                     DTAGVKDRKQSRSKYGTKKEKAKK"
     gene            complement(1249..2313)
                     /locus_tag="PLU36_09905"
     CDS             complement(1249..2313)
                     /locus_tag="PLU36_09905"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HMZ47106.1"
                     /translation="MTAAVDINVIKAESSKLKEMSLENIPFGKYFTDHMLEADYENGE
                     WKNVEIKPYQPLVIEPSMASLHYGQAIFEGIKAYKSPNGDAFIFRPKDNYNRFNVSAE
                     RMQMPAVPEDIFMEGMRMLVELDKSWIPGHTDHSLYIRPFMFSTDHLIGVRPSETYKF
                     MIILSPTGPYYNAPMKIYVEEKYTRAAPGGVGFSKNAGNYAASMYPTAMARKAGYDQV
                     LWTDAFEHKYLQEVGTMNVFFIIGNKAITPSLEDGTILAGVTRDSAITILKKMGLEVV
                     ERKITTDELIDVYKSGELKEVFGTGTAATISLIKELKYKDFIMEFNVDSWKVSTELKE
                     RLNGIRYGTATDEFGWMYKL"
     gene            2385..>2872
                     /locus_tag="PLU36_09910"
     CDS             2385..>2872
                     /locus_tag="PLU36_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47107.1"
                     /translation="MNEPISLSKYVIAKLYKDNIVITDKIEENLEPEKSADVNILFLG
                     ENKKNIVILLKEEDAMHLKDDSFQFLSSILQACKLNMADVAIINYNNQSINLNKVTKQ
                     FAPKFVIIFGELPNTIKQDATIDFYTPININSAVWLKAAVLDEMKENSTNAKVEKSKL
                     WN"
BASE COUNT          933 a          497 c          383 g         1059 t
ORIGIN      
        1 caacattcct gagaaacaaa aagactcagg ttgaatttga accagggtct gatacgtatc
       61 agaccttttt ttatgcaaaa aaaatggcta caaaatttgt aaccattact gtataataaa
      121 aaataagtaa attaaacttt aaagtgagag aatgctttat ttgcttctgc catacgatgt
      181 acatcttctt tctttttaaa tgcagcacct tctcctttac ttgcagatat aatttcactg
      241 gctaatttat ctgccattgt tttaccattt ctttcacggc tataacgaat taaccatttc
      301 atgcttaaag aaatttttct atctgcacga acttcacttg gtatttgaaa agtagcacca
      361 cctattcttc tgctacgtac ttcaacagct ggtgtaacat tggctaatgc ttttttccat
      421 acttcataac catcttctcc tgttatgctg ctaactttat ctacagcatc attaaaaatg
      481 gtaatggctg tgcttttttt accatcccac attaaattgt taataaaacg agttacctgt
      541 ttgtcgttaa atctagcatc tggtgctaaa ggcaactttt tggcttgtgc tttacgcatt
      601 ggttttaatt tttaagttta caaactgaat ttcttcaagt attaaaactt tactcagttt
      661 ataataatta ttgaaattga ttatttttta gctttttctt tctttgtacc atatttacta
      721 cggctttgtt ttctatcttt tacacctgca gtatctaaac taccacgtac aatatgataa
      781 cgtacacctg gtaaatcttt tacccttcca ccacgtatta aaactattga gtgctcttgc
      841 aaattatgac cttctccagg tatataagca attacttcaa ttttatttgt taaacgaact
      901 ttggcaactt tacgtaaagc tgagttaggt tttttaggtg tagtggtata tactcttgta
      961 caaacaccac gacgttgagg acaagcatct aaagctcttg atttgctttt tgctctaata
     1021 atttcacgac cttttcttac taattgttga attgtaggca ttgtaacctt ttatttttta
     1081 ggacggcaaa agtagtagtt tttgcttata atccaaaaga tttaataaaa tattattgtt
     1141 tttttttcac atttaatgta aaaaatttat tggaaaacag atttataccc atttattcac
     1201 aaaaagaggg caaaaaccct ctttttgata atttatataa agtaataatt ataatttata
     1261 catccatcca aattcatctg ttgcagtacc atatctaatt ccatttaatc tttcttttag
     1321 ttctgtagat accttccaac tatcaacatt aaattccata ataaaatctt tatatttcaa
     1381 ttcttttatt aaagaaatag ttgctgcagt tcctgtacca aaaacttctt ttagttcgcc
     1441 agatttatat acatcaatca attcatctgt tgtaattttt ctttcaacca cttccaaacc
     1501 cattttcttt aaaatagtta tggcactatc tctggttaca cctgctaaaa tagttccatc
     1561 ttctaatgat ggtgttatgg ctttatttcc tataataaaa aacacattca ttgtacccac
     1621 ttcttgtaaa tacttatgtt caaatgcatc tgtccataaa acttggtcat agccagcttt
     1681 tcttgccatt gcagttggat acatacttgc agcataattt cctgcatttt tgctaaaacc
     1741 aacaccacca ggtgctgctc ttgtatattt ctcttctaca taaattttca ttggggcatt
     1801 ataataaggg cctgttgggc ttaatataat catgaattta tatgtttcag aaggtctaac
     1861 accaattaaa tgatctgtag aaaacataaa tggcctgata tataaagaat ggtctgtatg
     1921 cccaggaatc cagcttttat ctaattcaac taacatacgc atgccttcca taaaaatatc
     1981 ttcgggtaca gcaggcattt gcattctttc ggcagataca ttaaatctat tataattatc
     2041 ttttggccta aatataaaag catctccatt aggcgattta taagccttta tgccttcaaa
     2101 aatggcctgt ccgtagtgta acgatgccat tgaaggttca ataactaatg gctgatatgg
     2161 tttaatttct acattcttcc actcaccatt ttcataatca gcttcaagca tgtgatcagt
     2221 aaaatatttc ccaaaaggaa tattttctaa actcatttct tttaatttgc ttgattcagc
     2281 cttaataaca ttaatatcta ctgctgctgt cataactaaa tatatttgct gcaaagaaac
     2341 gtaatttttt aaaactaaca attgttaata ttgcacatgt aattatgaac gaacctattt
     2401 cactatccaa gtatgtaatt gccaagttat ataaagataa tatagtaatt acagataaaa
     2461 ttgaggaaaa tttagaacct gaaaagagtg cagatgttaa tattttgttt ttaggcgaaa
     2521 acaaaaaaaa tattgttatt ctgttaaaag aagaagatgc tatgcattta aaagacgata
     2581 gttttcagtt tttatcatcc attttacaag cttgtaagtt aaatatggcc gatgtagcaa
     2641 taattaatta caacaatcaa tcaataaatt tgaataaagt aactaaacaa tttgccccta
     2701 agtttgttat tatttttggc gaattgccca atacaataaa gcaggatgct actatagatt
     2761 tttatactcc aattaatatt aatagtgcag tttggttaaa agctgctgtt ttagatgaaa
     2821 tgaaggaaaa tagtaccaat gctaaagtag aaaaaagcaa attatggaat tg
//
LOCUS       DAOQPK010000263         2854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_27236_length_2854_cov_4.138264, whole genome shotgun sequence.
ACCESSION   DAOQPK010000263 DAOQPK010000000
VERSION     DAOQPK010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27236_length_2854_cov_4.138264"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PLU36_09915"
     CDS             <1..225
                     /locus_tag="PLU36_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HMZ47108.1"
                     /translation="DALLNKAKEILQTVNSKAPLAVAACIKAANAVWKETVNGFDEEI
                     QLFGNCFGTNDMKEGTNAFLEKRKAVFTGN"
     gene            341..577
                     /locus_tag="PLU36_09920"
     CDS             341..577
                     /locus_tag="PLU36_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HMZ47109.1"
                     /translation="MSDIATRVKKIIVDKLGVDEAEVTTEASFTNDLGADSLDTVELI
                     MEFEKEFSISIPDEQAETITTVGQAVTYLEEHAK"
     gene            765..2018
                     /gene="fabF"
                     /locus_tag="PLU36_09925"
     CDS             765..2018
                     /gene="fabF"
                     /locus_tag="PLU36_09925"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HMZ47110.1"
                     /translation="MTLKRVVVTGLGAITPLGNNVETYWQNLVNGVSGADMITLFNAE
                     KFRTKFACEVKNFDPTEFLDRKEARKIDRFTQFALVVSDQAMNDAGLNKENINPDRIG
                     VVFASGIGGLITFQHEVQEFAKGDGTPRFNPFFIPKMILDIAAGQISMRHNLRGPNFA
                     VVSACASSTNAIMDAFNLLRLGKADIILTGGSEAVISEAGVGGFNAMKAMSERNDEPK
                     TASRPYDKDRDGFVMGEGAGMLVLEELEHAKARGAKIYCEVMGCGATADAHHITAPHP
                     DGLGAKNVMLAALEDANMQPTEIDYINTHGTSTPVGDGAEIKAVTEVFGEHAFKLNIS
                     ATKSMTGHCLGAAGALEAIASIMAVVHDTVPPTINHFTDDPEIDTQLNFTFNKAQQRT
                     VNAALSNTFGFGGHNACVIVKKYKA"
     gene            2025..2732
                     /gene="rnc"
                     /locus_tag="PLU36_09930"
     CDS             2025..2732
                     /gene="rnc"
                     /locus_tag="PLU36_09930"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HMZ47111.1"
                     /translation="MQFLKNIFNITSKTVFIKQVENVLGFKTHKSNLYITALSHRSIK
                     DTPDQNNERLEYLGDAILSSIVADYLFKRYPYKGEGFLTEMRSKMVNRNQLNDIALKM
                     GLKKITLYNKIDNSLRNSQIFGNTLEAVVGAVYLDKGYKKTQDWVLKQILIPHMFVDD
                     LENIDINLKNKLIGWANKNNKELAFEVAEEKIISGRRLFIINTVIDGEVLSQGSGYNK
                     KDASHTAAQLAIEKLGL"
BASE COUNT         1048 a          433 c          520 g          853 t
ORIGIN      
        1 gatgccctat tgaataaagc aaaagaaata ttacaaacag taaattccaa agctccttta
       61 gcagttgcag cttgcataaa agcagccaat gctgtgtgga aggaaacagt caacggtttt
      121 gatgaagaaa ttcaactttt tggcaattgc ttcggtacaa atgatatgaa agaaggcact
      181 aatgcatttt tagaaaaaag aaaagccgtt tttactggta attagccttt tagggcagta
      241 aaaaactaca ttttcttccc taattttaat aaaaattatt attaattttg cattttaact
      301 aaaagctcta tttttgccat tataactaaa actttttaaa atgtcagaca ttgcaacaag
      361 agtaaagaaa atcatcgttg ataagttagg tgtagatgaa gcagaagtaa ctacagaagc
      421 ttcttttact aacgacttag gtgcagattc tttggatact gttgaattaa tcatggaatt
      481 tgaaaaagaa ttcagcatct ctattcctga tgaacaagca gaaacaatta caaccgttgg
      541 gcaagctgtt acttacttag aagagcacgc caaataaaag atttactcca taattaattg
      601 gaataattaa cataaatccg ccattcatag atataattat cagagttggc ggatttattt
      661 atttaataaa ctttcttagc tttaatatta tatttgtagt attaacctgt tatgcatttt
      721 tttaattgct tagcaatgtt tataaaacta aaagcttatt gtatatgact ttaaaaaggg
      781 tagtggttac aggtcttggg gccataacac cattaggtaa taatgtagaa acttactggc
      841 aaaatcttgt aaatggtgtt tctggtgccg atatgattac tttatttaat gcagaaaaat
      901 ttagaaccaa atttgcctgc gaagtaaaaa actttgaccc taccgaattt ttagatagaa
      961 aagaagccag aaaaattgat aggtttacgc aatttgcctt agttgttagc gaccaagcaa
     1021 tgaacgatgc aggcttaaac aaagaaaata ttaaccccga tagaattgga gttgtatttg
     1081 ctagtggaat tggtggatta atcacctttc aacacgaagt acaagaattt gcaaaaggcg
     1141 atggaacgcc acgtttcaac ccatttttca taccaaaaat gattttagat attgctgcag
     1201 gccaaataag tatgaggcat aatttaagag gccccaactt tgctgtagta agtgcctgtg
     1261 caagtagcac caatgcaata atggatgcat ttaacttact gcgtttaggc aaagcagata
     1321 ttatactaac tggtggcagc gaagctgtaa ttagcgaagc tggtgttggt ggctttaatg
     1381 ccatgaaagc catgagcgaa agaaacgatg aacccaaaac agcatcaagg ccctacgata
     1441 aagatagaga tggttttgta atgggagaag gtgcaggtat gctggtattg gaagaattag
     1501 aacatgctaa agctcgtgga gcaaaaattt attgcgaggt aatgggttgt ggagcaactg
     1561 ctgatgctca tcatattact gctcctcacc cagatggctt aggggcaaaa aatgtaatgc
     1621 tggctgcttt agaagatgcc aatatgcagc caactgaaat tgattatatt aacacacacg
     1681 gcacaagtac gcctgttggc gatggtgcag aaattaaagc tgtaacagaa gtgtttggag
     1741 aacacgcttt taaactaaat attagtgcta caaaaagcat gactgggcat tgtttaggtg
     1801 cagcaggtgc attagaagcc attgcaagca ttatggctgt agtgcatgat acagttcctc
     1861 ctactatcaa tcactttaca gacgatcctg aaatagatac tcaattaaat ttcactttta
     1921 ataaagctca acaacgtaca gtaaatgctg cactaagcaa cacatttggc tttggtgggc
     1981 acaatgcttg tgtaattgta aaaaaatata aagcataaaa atttgtgcag tttttaaaaa
     2041 acatttttaa tataacttct aaaactgttt ttataaagca ggtagaaaat gtattaggat
     2101 ttaaaacaca caaaagtaat ttatacataa ctgcacttag ccatagaagc ataaaagata
     2161 ctccagatca aaataatgaa cgattagaat atttaggcga tgccatttta agcagcattg
     2221 tagccgatta tttgtttaaa agatatccat ataaaggcga aggattttta accgaaatgc
     2281 gtagcaaaat ggttaacaga aatcaactaa atgatattgc ccttaaaatg ggcttaaaaa
     2341 aaattaccct gtataataaa atagataata gcttacgcaa tagtcaaatt tttggtaata
     2401 cattagaagc agttgttggt gctgtgtatt tagataaagg atataaaaaa acacaagact
     2461 gggtgcttaa acaaatatta ataccacata tgtttgttga tgacttagaa aatattgata
     2521 ttaacttaaa aaacaaacta attggctggg ctaataaaaa taataaagag ttggcatttg
     2581 aagttgccga agaaaaaatt attagcggca gaaggctttt tattattaat acagttatag
     2641 atggggaagt tttatctcaa ggaagcggat acaataaaaa agatgcaagc catacagcag
     2701 ctcaactagc aatagaaaaa ctaggtttat aagtacccat ccaaaaaaaa atttacctac
     2761 ccattcgttt tataaattta taacactccc actttttcca ttaactatta attattcaac
     2821 attaaaattt attttttaat attgtacatc taaa
//
LOCUS       DAOQPK010000264         2852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_27284_length_2852_cov_3.894887, whole genome shotgun sequence.
ACCESSION   DAOQPK010000264 DAOQPK010000000
VERSION     DAOQPK010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27284_length_2852_cov_3.894887"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PLU36_09935"
     CDS             <1..582
                     /locus_tag="PLU36_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HMZ47112.1"
                     /translation="KGALIEVNTDLLAAARSLCGNQKGIACILGTGSNSCYYDGKKII
                     KSNPSLGFVLGDEGSGAYLGKKVLQYYLYQTFDEELTINFDKSYNTNKATILNSIYKS
                     NTPNRYLASFASFLSENRGHYMIENIIEDGLNDFFQNHIYKYRESWLHPIHFTGGIAY
                     TYKDVIKELCHSYELEVGNFSKSPMQGLLQYHQ"
     gene            complement(636..1103)
                     /gene="greA"
                     /locus_tag="PLU36_09940"
     CDS             complement(636..1103)
                     /gene="greA"
                     /locus_tag="PLU36_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HMZ47113.1"
                     /translation="MSEYVTQETFDKMKETLQKMKAIDRPAASKAIAEAREKGDLKEN
                     AEYDSAKEAQGLLEARIKQLEGAIATAKIVDTTSIDTSRVSILTKVTITNVATKKTVT
                     YQIVGEKEADLKMGKISSSSPIGKGLIGKQIGEIAEVQAPNGILKFKVEEIAI"
     gene            1290..>2852
                     /locus_tag="PLU36_09945"
     CDS             1290..>2852
                     /locus_tag="PLU36_09945"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ47114.1"
                     /translation="MIKSIVITLVNLLFINSLIAQSKIYGTVKGNKNKNLIAASITIV
                     GTYDGTITDSLGKYKITTYEKGQFTVEVKCLGYKTTTQPLYLDNKNVEINFILKEEIN
                     ELSAVTVTAGSFEAGDKKRTATVLTALDVYTTGGANGDITSAIKTLPGTQQVGEQEGL
                     FVRGGAGYETKQMIDGLVVNNPFFSGAPDIATRGRFSPSLFKGNVFSTGGYSALYGQA
                     LSSVLLLESIDMPERSEASASITSVFAGAGFQHLAKNKKSSIGINYGYTNLQPYFSVV
                     KQKQTFSIFPTYQNVEANFRIKTKRNGMIKFYSSFSDGKMGLRTIDIDSVTLKNQFEL
                     RNNNWYNIITYKENLGKGWKLNTGISFSTNTDKINNKVIDNNNQPTITGKIYIDSKNF
                     ILRTKQELTVLRAVAEKRIKGISVVRFGTEYWFANNEYSFNGFKTNFKDRFTSLFAEA
                     DIYLTNNLAFKAGSRFENSSLINKNKEKLPKQMPFTITKYKMSNKDTKTEMDIQNQEK
                     TINFALQAAEVLN"
BASE COUNT         1072 a          399 c          452 g          929 t
ORIGIN      
        1 aaaggggcat taatagaagt aaatacagat ttgttggctg ctgcaagatc tttatgtggt
       61 aaccaaaaag gtattgcttg tatattagga acaggctcca attcttgcta ttacgatgga
      121 aagaaaatta ttaaaagtaa cccttcctta ggttttgtgt taggagatga aggaagtggt
      181 gcttatttag gtaaaaaagt tttacagtat tatttatatc aaacttttga tgaagaacta
      241 actataaatt ttgataaaag ttacaatact aacaaggcta ctattttaaa ctcaatttat
      301 aaaagcaata cacccaatag gtatttagca agttttgcaa gttttttatc agaaaataga
      361 gggcattata tgatagaaaa tattattgaa gacggcttaa atgatttttt tcaaaatcac
      421 atttataagt atagagaaag ttggctacac cctattcatt ttactggtgg tatagcatat
      481 acatataaag atgtaattaa agaactttgc cacagttatg aattagaagt tggcaatttt
      541 agcaaaagcc caatgcaagg tttattacaa taccaccaat aaaaaagcct caatttaata
      601 attgaggctt ggagaatttt ttgaaagcta ttgctttaaa tggcaatttc ttctacttta
      661 aatttcaata taccattagg tgcttgcact tcggcaattt ctcctatttg cttgccaatt
      721 aaacccttac caattggaga agaggaagaa attttaccca tttttaaatc ggcttctttt
      781 tcaccaacaa tttgataagt aactgttttt ttagtagcta cattagttat agttacttta
      841 gttaaaatgc ttactctact agtatcaata gaggttgtat caacaatttt ggcagttgca
      901 atggcacctt ctaattgttt aattctagct tccaataaac cttgtgcctc tttagcacta
      961 tcatattcag cattttcttt taaatcgcct ttttctcttg cttctgcaat tgcttttgaa
     1021 gcagcagggc ggtcaatagc tttcattttt tgaagggttt ccttcatttt atcaaatgtt
     1081 tcttgtgtta catactcact cattgtacaa atatttacag cttataaaaa aaatacaacg
     1141 ctacatatac tttaatacat gaagcgttgc atgaactaca aatataaaga aaaatgaatt
     1201 aatttataga acaaaatttt ttaattaata aaaaataatt tggtttatat tataaactac
     1261 tttacgtttg tgctatcaaa agaagtaaaa tgataaaaag tatagttata acactagtta
     1321 atttattatt cattaattca ttaattgctc aatcaaaaat ttatggaaca gtaaaaggta
     1381 ataagaacaa gaatttaatt gcagcaagca ttaccattgt tggtacttat gatggtacca
     1441 taacagactc tttaggtaaa tataaaatta ctacttacga aaagggacaa tttactgttg
     1501 aggtaaaatg tttaggttat aaaaccacca ctcaaccatt atacttagat aacaaaaatg
     1561 ttgaaataaa ctttatttta aaagaagaaa ttaatgaatt gagtgcagtt acagtaactg
     1621 caggcagttt tgaagctggt gataaaaaaa gaactgctac cgtattaact gctttagatg
     1681 tttatacaac aggtggtgca aatggagaca ttactagtgc aataaaaact ttgccaggca
     1741 ctcaacaagt tggagaacaa gaaggcttgt ttgtacgtgg tggtgcaggc tacgaaacta
     1801 aacaaatgat tgatggttta gttgttaata atccgttttt ttctggtgca ccagatatag
     1861 caacacgtgg tagattttct ccatcattat ttaaaggcaa tgtatttagc acaggtggct
     1921 atagtgctct ttatgggcaa gcactaagca gtgttttatt attagaaagt attgacatgc
     1981 ctgaacgttc agaagctagt gcatctatca catcagtttt tgcaggtgcc ggttttcaac
     2041 atttagccaa aaacaaaaaa tcttccatag gaataaatta tggttatact aacctacagc
     2101 cttacttttc ggtagttaag caaaaacaaa cctttagtat ttttccaact tatcaaaatg
     2161 tagaagcaaa ttttagaatt aaaacaaaga gaaatggaat gattaagttt tattcaagtt
     2221 tttctgacgg caaaatgggt ttaagaacaa ttgatattga tagtgtaact cttaaaaatc
     2281 aatttgaatt aagaaataat aactggtata atattataac ttataaagaa aatttaggta
     2341 aaggttggaa actgaataca ggaattagct ttagcactaa taccgataaa ataaacaata
     2401 aagtaataga caacaataat cagccaacaa ttacaggtaa aatttatatt gattcaaaaa
     2461 attttatttt aagaacaaaa caagaactta cagtattaag agctgttgca gaaaagagaa
     2521 taaagggaat tagtgtagta agatttggta cagaatattg gtttgcaaat aatgaatata
     2581 gctttaatgg ttttaaaaca aatttcaaag accgcttcac atctcttttt gccgaagcag
     2641 atatttacct aaccaacaac cttgcattta aagcaggtag caggttcgaa aattcaagtt
     2701 taataaacaa aaacaaagaa aaattaccta aacaaatgcc ttttacaatt acaaaatata
     2761 aaatgagtaa taaagacaca aaaacagaga tggatataca aaatcaagaa aaaacaataa
     2821 attttgcttt gcaggcggca gaagtactga ac
//
LOCUS       DAOQPK010000265         2828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_27688_length_2828_cov_3.241976, whole genome shotgun sequence.
ACCESSION   DAOQPK010000265 DAOQPK010000000
VERSION     DAOQPK010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27688_length_2828_cov_3.241976"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1096)
                     /locus_tag="PLU36_09950"
     CDS             complement(17..1096)
                     /locus_tag="PLU36_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HMZ47115.1"
                     /translation="MFQIFQYKQLFHFTEQVFLKIGCSANDATIATKVLLNADIRGID
                     SHGVARLVGYVRLWQAKRINANPAISIIHQTPSTATVDGDKGLGLVVAPYAMQLAINK
                     AKECGTGWVAVQNSNHFGIAGYHAMMALEHDMIGIAMTNASALVAPTFSIEKMLGTNP
                     IAVAIPANKQPAFVADFATTTAANGKLEILQRKNLDTPLGWVQDKDGYPSTDAHILKN
                     DGALLPLGSDKEHGSHKGYALGSIVDIFSAVLSGASYGPWAPPFPAYIAMPQNMPGKG
                     LGHFFGAMRIDAFRSATDFKQHMDNWIERFRNAKPANGYEKVLIPGDPEREMQEERMN
                     NGIPVIEAVVKDLHNLAQTLDIALL"
     gene            complement(1114..2145)
                     /gene="ruvB"
                     /locus_tag="PLU36_09955"
     CDS             complement(1114..2145)
                     /gene="ruvB"
                     /locus_tag="PLU36_09955"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HMZ47116.1"
                     /translation="MSNPNLNKEGEQLSAAEREFENSIRPTIIDDFSGQPQLIENLII
                     FIKAAKIRGEALDHILFHGPPGLGKTTLSRIVANELGVNIKETSGPVIEKPGDLAGLL
                     TNLQPNDVLFIDEIHRLSTVVEEYLYAAMEDFRIDIMIDSGPNARSIQLNLNPFTLVG
                     ATTRSGLLTAPLLSRFGIKSRLEYYNAEVLKKIIERSSAILQTQISKDAAAEIARRSR
                     GTPRIANGLLRRVRDFAQVLNDGKIDIGITQHALKALNVDEHGLDEMDNRILQAIIEK
                     FKGGPVGITTIATAVGEEPGTIEEVYEPFLIQEGFLQRTPRGREVTLKAYEHLGKKPL
                     HGNQPNLFL"
     gene            complement(2220..>2828)
                     /locus_tag="PLU36_09960"
     CDS             complement(2220..>2828)
                     /locus_tag="PLU36_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47117.1"
                     /translation="IVAKAISYIFHPLFIPTYIFWFLTIYFPFEFPGITQKLLGLRIF
                     SVFWMTAFFPALSVFLLWRLKFIDNVFLRTQKERIIPFFITMFFYWWMYYLSRNFTDQ
                     PIALKFFFFGIFISTSVGVVINNYIKISLHGIAMGGALMAIILISNYYQINLSIPICV
                     AIMLTGMVASSRFIAGNHTDAEMYIGIVVGIVCQLFGYWFVL"
BASE COUNT          903 a          524 c          439 g          962 t
ORIGIN      
        1 aagagagtat aattagttat aataaagcta tatcaagcgt ttgtgctaaa ttatgtaaat
       61 ctttaaccac agcttcaatt accggtattc cattgttcat tctttcttct tgcatttctc
      121 tttctgggtc gccgggtatt aacacttttt cgtatccatt ggctggtttt gcatttctaa
      181 atcgttcaat ccagttatcc atgtgctgtt taaaatctgt tgcactacga aatgcatcaa
      241 ttctcattgc tccaaaaaaa tgacccaagc ctttaccagg catgttttgt ggcatagcaa
      301 tatatgcagg aaaaggaggt gcccaagggc catagcttgc accacttaat actgcagaaa
      361 aaatatctac aatacttcct aaagcatagc ctttatggct gccatgttct ttatcgctac
      421 ctagtggtaa taaagctcca tcatttttta agatatgagc atctgtagag gggtaaccat
      481 ctttatcttg aacccaacct aaaggtgtat ctaagttttt tctttgcaat atttcaagct
      541 taccattggc agcagtggtt gttgcaaaat ctgctacaaa agcaggttgt ttattggcag
      601 gtatggcaac agcaattggg ttggtgccca acattttttc tatagaaaaa gtgggtgcta
      661 ccaatgcact ggcattggtc attgcaatac caatcatatc atgctctaaa gccatcatag
      721 catgataacc tgcaattcca aaatgattac tattttgtac agcaacccaa cctgtaccac
      781 attcttttgc tttattaatg gctaattgca ttgcataggg agccactaca aggcctaacc
      841 ctttatctcc atctactgtt gcagtagatg gggtttggtg tattattgaa atggcagggt
      901 tggcattaat tcttttagcc tgccaaagtc ttacataacc tacaagcctt gctacgccat
      961 ggctgtcaat tcctctaata tcagcattta ataatacttt tgttgcaatg gttgcatcat
     1021 ttgctgagca gccaattttt aaaaaaacct gttcggtaaa atgaaataat tgtttatact
     1081 gaaatatttg aaacataata tttaaaacaa aaattataaa aataaattag gctgattacc
     1141 atgaagtggt ttttttccta agtgttcgta agcttttaaa gttacctctc tacctcttgg
     1201 tgttctttgt aaaaaaccct cttgtattaa gaatggttca tatacttctt caattgttcc
     1261 aggctcttcg cctactgcag ttgcaatggt agtaatacct acagggccac ctttaaattt
     1321 ttctattata gcttgtaaaa ttctgttgtc catttcatct aagccatgtt catctacatt
     1381 caaggctttt aaagcatgtt gtgtaatgcc aatatcaatt ttaccatcat ttaaaacctg
     1441 tgcaaaatct cttactcttc ttaataagcc attggcaata cgtggtgtac ctctgcttct
     1501 tcttgcaatt tctgctgcag catctttaga aatttgggtt tgtaaaattg cactacttct
     1561 ttcaataatt tttttcagga cttctgcatt ataatattct aatctgcttt ttattccaaa
     1621 tctgctgagt aatggtgctg ttaataaacc acttcttgtt gttgcaccta ctaaagtaaa
     1681 tgggtttagg tttaattgaa tacttcttgc atttggtcca ctatctatca ttatatcaat
     1741 tctaaaatct tccatagcag catataaata ttcttcaaca acagtactta atctatgaat
     1801 ttcatcaata aataaaacat cgtttggttg taaattggtt aataagcctg cgaggtcgcc
     1861 aggtttttct ataactggcc cactagtttc ttttatattt acacctaatt cattagcaac
     1921 aattctgctt aatgttgttt tacctaaccc tggtggtccg tgaaataaaa tatggtctaa
     1981 tgcttcgcca cgaattttgg cagctttaat aaaaatgatt aaattttcta ttagttgagg
     2041 ttgtccacta aagtcatcaa taatggtggg cctaatactg ttttcgaatt cacgttcagc
     2101 cgccgaaagt tgctcccctt ctttattcaa attaggatta ctcatattat ttcaaaacta
     2161 caacaatgaa agggtatgta aaaattaaaa cgtaattaat tgtataagca atgtatattt
     2221 tacagtacaa accaataacc aaaaagttgg caaactatac ctaccacaat accaatatac
     2281 atttctgcat cggtgtgatt gccagcaata aaacggctac ttgcaaccat gccagtaagc
     2341 ataatagcta cacaaatagg aatacttaag ttaatttgat aataatttga aattaaaata
     2401 atagccatta atgcaccacc catggctatg ccatgcaaac taatttttat gtaattattt
     2461 attactacac ctaccgaagt ggagataaat ataccaaaga aaaaaaactt taatgcaata
     2521 ggctgatctg taaaatttct gcttaggtaa tacatccacc aatagaaaaa catggtgata
     2581 aaaaaaggta ttattctttc tttttgtgta cgtaagaaaa cgttatcgat gaactttaaa
     2641 cgccacaaca aaaaaacaga taaagctggg aaaaaagctg tcatccaaaa tacactaaat
     2701 attcttaaac ctaatagttt ttgtgtaatt ccaggaaact cgaatgggaa gtaaatggtt
     2761 aaaaaccaaa aaatataagt tggtataaat aatggatgaa aaatatagct gattgctttt
     2821 gctactat
//
LOCUS       DAOQPK010000266         2772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_28726_length_2772_cov_4.296651, whole genome shotgun sequence.
ACCESSION   DAOQPK010000266 DAOQPK010000000
VERSION     DAOQPK010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28726_length_2772_cov_4.296651"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..>2772
                     /gene="sprA"
                     /locus_tag="PLU36_09965"
     CDS             167..>2772
                     /gene="sprA"
                     /locus_tag="PLU36_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HMZ47118.1"
                     /translation="MFAQTNKSTDTSKKVNNDSLHYPIKDRWGDRYTWQNKNPFNIRD
                     TSLLKQQIEYDPITKRYYIIEKIGSKTYRTPTYLTFEEFQKIQNYQAEVEYFKHRAQA
                     LTSLNRKVLRPKMRVFDKLFDRIFGLGKDGFKVDIKPQGSVDILAGYQGQITKNPTLP
                     EAARKNGGFDFDMNANLNVNANIGDKLKLPISYNTLANFDFENQLKLDYKGMDDEILK
                     SVEAGNISWQSKGTLIPSTQNLFGLKTQLQFGKLFLTGAIANQRSIRQSQSLQGGSAT
                     TPFQKKLDDYEENRHFLLGQYFKNNFNTAMRNLPVVNSQVQIQRIEVWVTNRTGATTD
                     ARDVVGFADFGESNPSNAVNLPGNGTPSGLPGNQANALYNKLIAGGMGSPFRQPSQVT
                     SQLQALGLKPVDDFEKTYARKLTTNEYYFNPQIGFLSINTQLQPDEVLAVAYQYTYNG
                     QVYQVGEFSQDIALDSTQGVQKILFLKLLKATSARVNLPIWSLMMKNVYSLDVPGISR
                     EDFQLNILYEEPSGGLKRYLPQSATNVEGKPLLRILNLDRLNNRNDPQPDGQFDYIEG
                     YTILPQHGKIIFPVLEPFGKDLMQLAFNNGIGLPPGVDPTKYVYQILYDSIKAIAQTY
                     ANYNRFVMQGQAKGTGGSDIYLGAFNIPQGSVKINAGGQVLTEGVDYTIDYNLGSVKI
                     VNQAILNSGIPVSVSYENNAGFGMQQRGFLGLRADYLASKKLSLGLSMVRLGERPFFT
                     KMNYGEDPIRNTMYGFDFSYKTESPGLTRLVNKLPFYNSNAKSTITAYGEAAYLKPGH
                     PKQIGKGNEGLIYIDDFEGTRNSFDLRFPSIAWTLASTPTGKFAEASLFDSIDYNFNR
                     AKIAWYNIEPNL"
BASE COUNT         1028 a          422 c          482 g          840 t
ORIGIN      
        1 aaataaataa tattgaagaa ttaattaaaa acgctttaaa aaatttgtag tttatagatt
       61 tgatttttta cttaaccaag gtgtaggaat tttgtatttt actctatata ataattgcct
      121 taaaaggctg ttcgtatttt ttgcatttct atgcagtagc actgctgtgt ttgcacaaac
      181 caataaaagt acagatactt caaagaaagt aaataacgac tctttacatt atcctataaa
      241 agatagatgg ggcgatagat atacctggca aaacaaaaac ccatttaata ttagagatac
      301 atccttactt aaacaacaaa tagagtacga ccctataaca aaaaggtatt atattattga
      361 aaaaattggt agtaaaactt accgaactcc tacctattta acttttgaag aatttcagaa
      421 aatacaaaac taccaggctg aagtagaata ttttaaacac cgtgctcaag ctttaacctc
      481 tttaaacaga aaagtattaa gacctaaaat gagggttttt gataaacttt ttgatagaat
      541 ttttggttta ggaaaagatg gttttaaagt agatattaaa ccacaaggct ctgtagatat
      601 tttagcaggg tatcaaggtc aaataaccaa aaatccaaca ttgcccgaag cagcaagaaa
      661 aaatggtggc tttgattttg acatgaatgc caatttaaat gtaaatgcaa atattggcga
      721 taaacttaaa ttaccaatta gctataatac ccttgctaat tttgattttg aaaaccagtt
      781 gaaattagac tacaaaggca tggatgatga aatattgaaa agtgtggaag ctggcaatat
      841 ttcatggcaa tcaaaaggta cattaatacc aagcacacaa aatttatttg gattaaaaac
      901 gcaattacaa tttggtaaat tatttttaac aggtgcaata gctaatcaaa gaagcataag
      961 gcaaagccaa agtttacaag gcggttccgc aaccactcct tttcaaaaaa agttagatga
     1021 ttatgaagag aacagacatt ttttactagg tcagtatttt aaaaataatt ttaatactgc
     1081 aatgcgtaat ttaccagttg taaatagcca ggtacaaata caacgtattg aagtttgggt
     1141 aacaaataga acaggagcaa caacagatgc aagagatgta gtaggttttg cagattttgg
     1201 cgaaagtaac ccatcgaatg ctgtaaactt accaggcaat ggcacaccaa gtggtttacc
     1261 aggcaaccag gcaaatgctt tgtataacaa attaattgca ggtggtatgg gatctccttt
     1321 caggcagcct tctcaagtta caagtcaatt acaagctttg ggtttaaaac ctgtagatga
     1381 ttttgaaaaa acttatgcca gaaaacttac taccaacgaa tactatttta atccgcaaat
     1441 aggattttta tctattaaca cacaattgca accagatgaa gttttagcag ttgcctatca
     1501 atatacttac aatggccaag tgtaccaagt aggagaattt agccaagata ttgccttaga
     1561 ctctacacaa ggtgtgcaaa aaatattatt cttaaaatta ttaaaagcaa cttctgcaag
     1621 agttaattta ccaatatgga gtttaatgat gaagaatgtg tattcattag atgtaccagg
     1681 tattagcaga gaagattttc aattaaatat tttatatgaa gaacctagtg gtggtttaaa
     1741 aagatattta ccacaaagtg caaccaatgt agagggtaag ccattactta gaattttaaa
     1801 tttagataga ctaaacaaca gaaacgaccc acaaccagat ggacaatttg attacattga
     1861 aggctatacc attttaccac agcatggaaa aataattttt cctgttcttg aaccatttgg
     1921 taaagattta atgcagcttg cttttaataa tggcataggc ttaccacctg gtgtagatcc
     1981 tacaaaatat gtgtatcaaa ttttatacga ttctattaaa gcaatagcac aaacttatgc
     2041 aaactataat aggtttgtta tgcaagggca ggcaaaaggt accggtggca gcgatattta
     2101 tttaggagcc tttaacatac cacaaggctc agtaaaaata aatgcaggag ggcaagtatt
     2161 aacagagggt gtagattata ccattgatta taatttaggt agtgtaaaaa ttgtaaatca
     2221 ggcaatatta aattctggca ttccggtaag tgtttcttac gaaaacaatg ctggttttgg
     2281 aatgcagcaa agaggatttt taggcttaag agcagattat ttagcaagca aaaaattaag
     2341 tctaggttta tctatggttc gtttgggcga aagaccattt tttaccaaaa tgaattatgg
     2401 agaagaccca ataagaaaca ccatgtatgg ttttgatttt agctacaaaa ccgaatcgcc
     2461 agggttaact agattggtaa ataagttacc tttctataac tctaatgcta aaagtactat
     2521 tacagcttat ggcgaagctg cttacttaaa accaggtcac ccaaaacaaa ttggtaaagg
     2581 taacgaaggt ttaatttata tagatgattt tgaaggcaca agaaatagct ttgatttacg
     2641 tttcccatct attgcatgga ccttagcctc tacaccaaca ggtaaatttg cagaagcctc
     2701 tttatttgat tctattgatt ataatttcaa ccgagctaaa attgcttggt ataatattga
     2761 gcctaatttg ca
//
LOCUS       DAOQPK010000267         2770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_28758_length_2770_cov_4.598158, whole genome shotgun sequence.
ACCESSION   DAOQPK010000267 DAOQPK010000000
VERSION     DAOQPK010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28758_length_2770_cov_4.598158"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..424)
                     /locus_tag="PLU36_09970"
     CDS             complement(8..424)
                     /locus_tag="PLU36_09970"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HMZ47119.1"
                     /translation="MSNRTFTMIKPDATAKGFTGGILEMMLKAGFSIKAMKWTKLSTE
                     QAGAFYAIHKERPFYRELVEFMSSGPIVAAILEKENAVADFRTLIGATNPANAEEGTI
                     RKKYAASVGENAVHGSDSDENAIIESNFFFSELERY"
     gene            complement(536..1084)
                     /locus_tag="PLU36_09975"
     CDS             complement(536..1084)
                     /locus_tag="PLU36_09975"
                     /inference="COORDINATES: protein motif:HMM:NF025946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMZ47120.1"
                     /translation="MHFNDYLQYFETILNNTQNLQEPYNDPEFMEYTKLNWSRLNRWI
                     KTGELLDETKKTIQNIQTKQRWVLISEPWCGDAAHAVPFIVMMANLNPNITLEIQLRD
                     SGSEIEKYLTNGSKSIPLLIIRDLSGNDITKWGPRPKDCQADFSKLKAEGLEMADLKI
                     ALQKWYNNDKGVKIQQEIIALL"
     gene            complement(1262..2524)
                     /locus_tag="PLU36_09980"
     CDS             complement(1262..2524)
                     /locus_tag="PLU36_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012792322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HMZ47121.1"
                     /translation="MERFQGLIGIVLILGIAFLFSNNKKRINYRLVISGILLQVAIAL
                     LIFKVPPVTRFFQMLGHGMEKIEKFAREGASFVFGGIMTQQYDGTAANYVKGGFIFAF
                     NVTATIILVCVLVAIFYHAGIMQRIVAIIAKAMNFIMKVSGAEALSNVASAFVGQIEA
                     QVMIRPYLPTMTKSELLASMSGSLACIAGSILIVYANMGAAAGMDLAPKLITASLMAA
                     PGALVISKIVFPETEESQTMGKVKLEVKSNYTNLIDAISHGASDGFKISMNVIAMLIG
                     FIALITCVDYILLKTGHLFSANFDLSLNYIFGKIFYPMAWSMGVPAQDVQSAATLLGQ
                     KITVNEFVAFTNLTGKTVPIVTEKGLLIVSIAICGFANFSSVGMQIGGIGEIAPNRRT
                     DLAKLGLKALLCGTLASYLSATLAGIFI"
BASE COUNT          961 a          470 c          369 g          970 t
ORIGIN      
        1 actattatta atatctttct aattcagaga aaaagaaatt gctttcaata attgcatttt
       61 catcgctatc gctaccatgt acagcatttt cgccaacaga ggctgcatac ttttttctga
      121 tagttccttc ttcggcattt gcaggattag tagcaccaat taaagttcta aaatcggcaa
      181 ctgcattttc tttttctaaa attgcagcaa ctattggtcc actactcata aactctacta
      241 attctcgata aaaaggacgt tctttatgaa tagcataaaa tgcaccagct tgctcggttg
      301 atagcttagt ccatttcatt gctttaatgc taaaaccagc ttttaacatc atttctaaaa
      361 taccacctgt aaaacctttt gcagtagcat cgggtttaat cattgtaaaa gttctattac
      421 tcattttttt tattttcaat tgccgcaaaa gtagtgttat ataatttaca ccaaaataat
      481 agttaacagc tttatgattt tataaagaaa atgaataata aaagtgttat atcttctaca
      541 ataaagcaat tatttcttgc tgaattttta ctcctttatc gttattatac catttttgta
      601 gagcaatttt taaatcagcc atttctaatc cttcggcttt tagtttacta aaatctgcct
      661 ggcagtcttt aggtcttggt ccccatttag taatatcatt tccacttaag tctcttataa
      721 taagtaatgg aatagattta ctgccattgg ttaaatattt ttcaatttca gagccactat
      781 ctctcagttg tatttctaaa gtaatattgg ggtttaagtt tgccatcatt actataaatg
      841 gcacagcatg tgcagcatcg ccacaccaag gctctgaaat taatacccaa cgttgtttgg
      901 tttgaatatt ttgaattgtt ttttttgttt catctaataa ttcacctgtt tttatccagc
      961 ggtttaaacg agaccaattt aacttggtat attccataaa ctcaggatcg ttatatggtt
     1021 cttgcagatt ttgtgtattg tttaaaatgg tttcaaaata ctgtaaatag tcattaaaat
     1081 gcataattgt tattttattt atataaatca ggataattaa gtctttataa attatttgca
     1141 aataaataga ataaaactga acatacgtgt aacaaaagtt acaacatatt ttgtcataca
     1201 aacaaaaact ctcaatagtt tatattgaga gtagtttata aattgaatta acttaaataa
     1261 tttaaataaa aattccagct aaagttgcag aaaggtaaga ggccaaagta ccgcataaca
     1321 atgcttttaa gccaagcttc gctaaatcag ttcttctatt aggtgcaatt tctccaatac
     1381 caccaatttg cataccaaca ctgctaaagt tagcaaagcc acaaattgca atacttacaa
     1441 ttagtaatcc tttttcggta actataggaa ctgttttacc tgttaagttg gtaaaagcaa
     1501 caaattcgtt tactgtaatt ttttgaccta gtagggtagc agcactttgt acatcttgtg
     1561 caggaacgcc cattgaccat gccataggat aaaatatttt accaaaaata taatttaaac
     1621 ttaagtcgaa attagcacta aataaatgac cagttttaag taaaatataa tcaacacaag
     1681 taattaaagc aataaaacct attagcattg caataacatt catagaaatt ttaaaaccat
     1741 cgctagctcc atgagaaatg gcatcaatta aattagtata attacttttt acttctaatt
     1801 ttactttacc catagtttgg ctttcttctg tttcgggaaa aacaatttta gaaataacca
     1861 aagcccctgg tgcagccatt aaacttgcag taattagttt aggggctaaa tccatgccag
     1921 cagcagcacc catattggca tacacaatta atatactacc agcaatacag gcaaggctac
     1981 cactcatact tgctaataat tcacttttag tcatagttgg taaatatggc cttatcatta
     2041 cttgagcttc aatttgacca acaaatgcac tggcaacatt acttaaagct tctgcaccac
     2101 ttactttcat aataaaattc attgctttag ctataattgc aacaatacgt tgcataatac
     2161 ctgcatggta aaatattgca accaatacac aaactagaat aatagtagca gtaacattaa
     2221 aggcaaaaat aaaaccgcct tttacatagt tggctgctgt accatcgtac tgttgagtca
     2281 ttataccacc aaatacaaaa gatgctcctt ctcttgcaaa cttttcaatt ttttccattc
     2341 catgacctaa catttgaaaa aatctggtaa ctggtggaac tttaaaaatt agtaatgcta
     2401 ttgcaacctg aagtaaaata ccactaataa ctaaacgata attaattctt tttttgttgt
     2461 tggagaataa aaaagcaatt cctaaaataa gtactatacc tattaagcct tgaaatcttt
     2521 ccataagaaa tgttggttgt tagttttgtt ttaataagat tgaaagataa gataaaataa
     2581 aatatcaaaa tgaaaatttt tatataatac tatcaattaa aaaattttga aaatgtatac
     2641 ttcaacttta tcaattaaac tattgtacat tagctgcttt aaataagtac tgcttacatg
     2701 acttttgaaa aattattgca acagagttta gtgtggcgtg ggttatactt tttttcttta
     2761 ttattagtaa
//
LOCUS       DAOQPK010000268         2747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_29171_length_2747_cov_5.407504, whole genome shotgun sequence.
ACCESSION   DAOQPK010000268 DAOQPK010000000
VERSION     DAOQPK010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29171_length_2747_cov_5.407504"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /locus_tag="PLU36_09985"
     CDS             complement(<1..1347)
                     /locus_tag="PLU36_09985"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HMZ47122.1"
                     /translation="MSVQNNILSSPIEYLKGVGPLRADWLKKELNIFTFNDLLHLFPY
                     RHIDKSTITAINNIETNAEYIQVVGKLTNLQIIGERKGKRLTATLKDETGSIELVWFQ
                     GISWIQKILTEGSIYLVFGKLSYFNNTPQIIHAEIELYVPLQNNERKYFEPVYPSTEK
                     LKAKGLNGRQIAKLTFALWQQITIESIPENIPENILTELNLLNRYKAYKSIHYPANKI
                     EYNQALNRLKFEEFFIAQVRLNLVKIQRHQCSKGVIFEKVGALFNNFYSNHLPFELTN
                     AQKNVLKQIRQDAAKGHQMNRLLQGDVGSGKTIVALLSMLLAADNGYQSCLMAPTEIL
                     AMQHYNSLNSLLQPMNISVSLLTGSTKAKERKAILTDLQEGKIKFIVGTHALIEENVQ
                     FNNLGFAVVDEQHRFGVAQRAALWKKSVIPPHILVMTATPIPRTLAMTAYGDLDYSI"
     gene            1521..2372
                     /gene="panC"
                     /locus_tag="PLU36_09990"
     CDS             1521..2372
                     /gene="panC"
                     /locus_tag="PLU36_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HMZ47123.1"
                     /translation="MVICKTAAILENTLKKYYTNNINIGFVPTMGALHNGHIALIEES
                     KQQCAITVASIFVNPTQFNDKNDFEKYPITIENDIFLLEKAGCDIIFLPSVNEIYSDG
                     IDNLKHYELSYLEEILEGKYRKGHFQGVCNVVERLLLMVKPTILFLGQKDYQQCMVVS
                     KLVELMNWQNKLKLVIVPTIREENGLAMSSRNRRLTEEERTKAAAIYNNLLYMKNNFE
                     RLPFNGLIKRAEEELLKNGFLKIDYISICNAQNLQPIAEYKEGVKLVVLIAAFIGEVR
                     LIDNLLL"
     gene            complement(2411..>2747)
                     /locus_tag="PLU36_09995"
     CDS             complement(2411..>2747)
                     /locus_tag="PLU36_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HMZ47124.1"
                     /translation="ERMNYVTLVSCQLETGRTHQIRVHMKFIGHTLFNDWEYGGDKIL
                     KGTIFTKYKQFVDNCFAICPRCALHAKTLGFIHPTTQKEMFFEAELPEDMKNVISKWR
                     GYVGNKQLD"
BASE COUNT          903 a          397 c          432 g         1015 t
ORIGIN      
        1 aatactataa tctaagtcgc cataagctgt catagctaat gttctgggta tgggtgttgc
       61 agtcattacc aaaatgtgag gtggtataac agactttttc caaagtgcag cacgttgtgc
      121 aactccaaag cgatgttgct catcaacaac tgcaaatcct aaattgttaa actgaacatt
      181 ctcttcaatt aaggcatgcg tacctacaat aaattttatt tttccttctt gtaaatcggt
      241 taaaattgct tttctttctt tggcttttgt actacctgtt aataaactta cagatatatt
      301 cattggttgt aataagctgt ttaaactatt gtagtgctgc atagctaaaa tttctgtagg
      361 cgccattaaa caactttgat aaccattatc tgctgctaat aacatactaa gtaatgctac
      421 aattgttttt ccacttccaa catcgccttg taataaacga ttcatttgat ggcctttggc
      481 agcatcttgt ctaatttgtt ttaatacatt tttttgtgca ttggttaatt caaaaggtaa
      541 gtggttgctg taaaaattat tgaataatgc acctactttt tcaaatatta ctcctttgct
      601 acattggtgc ctttgtattt taactaagtt taatcttacc tgtgctataa aaaattcttc
      661 aaactttaat ctgtttaaag cttggttgta ttctattttg tttgcaggat aatgaataga
      721 tttatatgct ttatatctat ttaataaatt taattcggtt aaaatgtttt ctggaatatt
      781 ttctggaata ctttcaatgg taatttgttg ccataatgca aacgtaagtt tggcaatttg
      841 tctgccatta agacctttgg cttttagttt ttctgttgat ggataaactg gttcaaaata
      901 ttttctctca ttattttgta gtggtacgta caattcaatt tctgcatgaa tgatttgtgg
      961 agtgttgtta aagtaggaga gttttccaaa aaccaaatag atacttcctt ctgttaaaat
     1021 tttttgaatc caactaattc cctgaaacca aactaactca atacttcctg tttcatcttt
     1081 taaagttgca gtaagccttt tccctttcct ttcgccaatt atttgtaagt tggtaagctt
     1141 tccaacaacc tgtatatatt ctgcatttgt ttcaatatta ttaatagcgg tgatggtact
     1201 tttatcaata tgcctgtatg ggaaaagatg caataaatcg ttgaaggtaa aaatattcaa
     1261 ttcttttttc aaccaatcgg ctcttaaagg tccaactcct tttagatatt ctattggtga
     1321 tgataatatg ttattttgta cactcaattt atcaataaat agttctttgc aatgttacaa
     1381 tacctttagc tattaatact ttataaaaaa gctaaaataa tattgtttat acaatatttt
     1441 gcttttgaat taagaaggtg gaaagtaata gcatatactt agttttgttc ttctttttgt
     1501 aataaataat atgctttaaa atggttattt gtaaaactgc agcaatactt gaaaatacgc
     1561 taaaaaaata ttatactaac aatataaata ttggttttgt gccaacaatg ggagctttgc
     1621 ataatggaca tatagctttg atagaagaaa gcaaacaaca atgtgcaata acagtagcaa
     1681 gcatatttgt aaaccctaca cagttcaatg ataaaaacga ttttgaaaaa tatcctatta
     1741 ccattgaaaa cgatattttt ttattagaaa aagctggttg tgatatcatt tttctccctt
     1801 ctgtaaatga aatttattcg gatgggattg ataacttaaa acattatgaa ttgagttatt
     1861 tggaagaaat tttggaaggt aaatacagaa aaggtcattt tcaaggtgta tgtaatgtag
     1921 tggaaagatt gctgctaatg gttaaaccca ctatattgtt tttaggtcaa aaagattatc
     1981 aacaatgtat ggttgtatca aaattggttg agttaatgaa ttggcaaaac aaacttaaat
     2041 tagttattgt acctaccata agagaagaaa atggattagc catgagcagt agaaatagaa
     2101 gactaacaga agaagaaaga actaaggctg cagctattta taataactta ctgtacatga
     2161 aaaataattt tgaaagattg ccttttaatg gcttaatcaa gagggcagaa gaagaattac
     2221 taaagaatgg ttttttgaaa attgattata taagcatttg taatgctcaa aatttacaac
     2281 ctattgcaga atataaagaa ggggtaaaat tagttgtact aattgcagca tttattggcg
     2341 aagtaagatt aattgataat cttttattgt aatgctacag ttattattta agtaagttag
     2401 ggcacaagta ttagtctaat tgtttgtttc caacatagcc tctccatttg ctaattacgt
     2461 ttttcatatc ttcaggaagt tccgcttcaa aaaacatttc tttttgtgta gttgggtgta
     2521 taaagcccaa tgttttagca tgtaaggcac acctagggca aatagcaaaa caattatcta
     2581 caaattgttt gtatttggta aaaatggtac cttttaaaat tttatctcct ccatactccc
     2641 aatcattaaa taaagtatgg ccaataaatt tcatgtgcac tctaatttgg tgtgttcgcc
     2701 ctgtttctaa ctggcaactt actaaagtaa catagttcat tctttct
//
LOCUS       DAOQPK010000269         2731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_29505_length_2731_cov_4.245516, whole genome shotgun sequence.
ACCESSION   DAOQPK010000269 DAOQPK010000000
VERSION     DAOQPK010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29505_length_2731_cov_4.245516"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="PLU36_10000"
     CDS             complement(<1..808)
                     /locus_tag="PLU36_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HMZ47125.1"
                     /translation="MGLFSFFTQEIAMDLGTANTLIIHNEDIVVNEPSIVALNRNNPK
                     EVLAVGKKALLMHEKTHESIRTVRPLKDGVIADFNAAELMIRELIKLVYPKKPLFPPS
                     WRMVICIPSSITEVEKRAVRDSAEQAGAKEVYMIHEPMAAALGIGIDVEEPVGNMIID
                     IGGGTTGITVIALAGIVCDQSIRVAGDEFTADIMEALRRYHSLLIGERTAEQIKIHVG
                     AAIKDLDSPPDDMPVNGRDLVTGIPKQIMVSYQEIAEALDIPVKELFDFEI"
     gene            complement(1035..1784)
                     /locus_tag="PLU36_10005"
     CDS             complement(1035..1784)
                     /locus_tag="PLU36_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ47126.1"
                     /translation="MKVIPLSEGAFTIDKTKIFVPFNLKVDDLQQRPIGSLLVEVQPF
                     CIVTNQDILLIDTGLGFSKDGEPQLYSNLKQNGIQPQQITKVILSHLHKDHAGGISFR
                     NNIGHYSLSFPNATYYVQKKEFEFAFETGYPSFITDEFAILHNSPNVTWLNGNGNINN
                     YIYYQITGAHSPYHQVFFIKENNETIFFGGDEAPQLQQMKSKFVAKYDFDGRKSMELR
                     QDWWQQGNEENWQFLFYHDIKKPMYQNQPTV"
     gene            complement(1810..>2731)
                     /locus_tag="PLU36_10010"
     CDS             complement(1810..>2731)
                     /locus_tag="PLU36_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HMZ47127.1"
                     /translation="TALDKHIDAILKYPLVDVSAIKKAKFKIVIDAINSSGAFAVPAL
                     LKALGVNLKDIVVLNEEVNGKFAHNPEPLPQHLSELCNAVQKQNAHIGIAVDPDVDRL
                     CFVCEDGSLFGEEYTLVAVSDFVLKNKKGNTVSNMSSTRALKDVTEKHGGTHYPSAVG
                     EVNVVNKMKEVNAVIGGEGNGGIIVPDLHYGRDALIGIALFLTHLANEKKSVRQIRNT
                     YPNYFISKNKIDVENGIDVKKIFTEIKKKYKNNPINTEDGLKIDFDNDWVHLRTSNTE
                     PIIRIYSESNFETTADNIAKRIMHDIKEIM"
BASE COUNT          841 a          484 c          371 g         1035 t
ORIGIN      
        1 tgatctcaaa atcaaataat tctttgaccg gtatatctaa tgcttctgca atttcttgat
       61 agcttaccat aatttgttta ggaataccag taactaaatc tctaccattt acaggcatat
      121 catctggtgg gctgtctaaa tcttttatag ctgctcctac atgaatttta atttgttctg
      181 cggtacgctc accaattaat aaactatgat aacggcgtaa agcttccata atatctgcag
      241 taaactcatc gcctgcaaca cgaatgcttt ggtcgcaaac aatacctgct aatgcaataa
      301 ctgtaatacc tgttgttcct ccaccaatat caataatcat atttcctact ggttcttcca
      361 catcaatacc aatacctaag gctgctgcca ttggctcgtg tatcatatac acttcttttg
      421 ctcctgcttg ctctgcacta tctcttacag cacgtttttc aacttctgta attgatgatg
      481 gtatgcaaat taccattctc caacttggtg ggaaaagtgg ttttttagga tataccaatt
      541 ttattaattc tcttatcatt aattcggctg cattaaaatc ggcaattaca ccatctttta
      601 atggacgaac tgttctaata ctttcatggg ttttttcatg cattaataat gcctttttgc
      661 caacagctaa aacttctttt ggattatttc tatttaaagc tactatgctt ggttcattaa
      721 caacaatatc ttcgttatga ataattaatg tattggcagt gcccaaatcc atagcaattt
      781 cttgtgtaaa aaagctaaaa agtcccatgt attaaaggtt atataatttt ttaaaataat
      841 agcgaacaaa ggtgaatgaa ataacttgaa ttaacaaagg aaaaagcagt gttttttaca
      901 atgtttcttc attttttgcc taaattaact gttttatttc ttttatgaat aattattaaa
      961 aatgttttat aaaaagaaat gttgcgattt acttttttag ggaagaaagg tattaaactt
     1021 aaattgaata attgctacac agtgggctga ttttgataca ttggtttttt tatatcgtga
     1081 taaaagagaa actgccaatt ttcttcgttg ccttgttgcc accaatcttg tcttaattcc
     1141 atactttttc taccatcaaa atcatatttt gctacaaatt tacttttcat ttgttgtagc
     1201 tgtggtgcct catctcctcc aaaaaatatg gtttcgttat tttctttaat aaaaaaaact
     1261 tgatggtaag gactatgagc tcctgtaatt tggtaataaa tgtaattatt tatattccca
     1321 tttccattta accaagttac attagggctg ttgtgaagta ttgcaaattc atctgtaata
     1381 aaggatgggt aacccgtttc aaaagcaaat tcaaattctt ttttttgaac ataatatgta
     1441 gcattaggaa aggataaact ataatgtcca atattatttc taaaactaat accaccagca
     1501 tggtctttat gcaaatggct taatattact ttagttattt gctgtggctg aatgccattt
     1561 tgtttaaggt tggaatatag ttgaggctct ccatctttac taaaacctaa gcctgtatca
     1621 attaataaaa tatcttggtt agttacaata caaaagggtt gtacttctac caataagcta
     1681 ccaatagggc gttgttgtaa gtcatctacc tttaaattaa atggtacaaa tatttttgtt
     1741 ttgtcaatgg taaatgctcc ttcagataat ggtataactt tcattataaa taatgcattg
     1801 gttaaatgct tacattattt ctttaatatc atgcattata cgttttgcaa tattatctgc
     1861 tgtagtttca aaattgcttt cgctgtaaat acgtataatt ggctcggtat tacttgttct
     1921 taaatgcacc caatcgttat caaaatctat ttttaaacca tcttcggtat taattgggtt
     1981 atttttatat ttcttcttta tttctgtaaa tatttttttt acatcaatcc cgttttctac
     2041 atctatttta tttttactga taaagtagtt aggataagta tttcttattt ggcgaacact
     2101 cttcttttca ttggctaaat gtgttaaaaa caaagcaata cctataagtg catcacgacc
     2161 ataatgcaaa tctggtacaa taattccacc attgccttct ccaccaatta cagcatttac
     2221 ttctttcatt ttattcacca catttacttc gcctacagca cttggataat gtgtaccacc
     2281 atgtttttct gtaacatctt tcaacgctct ggtactgctc atattgctca cagtatttcc
     2341 ttttttgttt tttaaaacaa aatcagaaac tgctactaaa gtatattctt ctccaaataa
     2401 actaccatct tcacaaacaa agcaaagtct atctacatca gggtcaactg caattccaat
     2461 atgtgcattt tgtttttgca cagcattgca taactctgat aaatgttgag gtaagggttc
     2521 tggattatgg gcaaattttc cgtttacttc ttcgtttaaa acaacaatat cttttaaatt
     2581 aactcccaaa gcttttaata aagcaggtac agcaaatgct ccactgctat taattgcatc
     2641 tataacaatt ttaaatttag cttttttaat agcacttaca tctaccaacg gatattttaa
     2701 tattgcatca atatgtttgt ctaaagcagt t
//
LOCUS       DAOQPK010000270         2698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_30149_length_2698_cov_3.965191, whole genome shotgun sequence.
ACCESSION   DAOQPK010000270 DAOQPK010000000
VERSION     DAOQPK010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30149_length_2698_cov_3.965191"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PLU36_10015"
     CDS             complement(<1..286)
                     /locus_tag="PLU36_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47128.1"
                     /translation="MKRSKQYLLFILLFGAFINLGSYNSVFSQIFDHNGNTLNGTGST
                     STNSGIDEPVFEDNSTSTGNTAANGGGFAGTTSVDADDDDPGGPIDPIDDI"
     gene            complement(441..677)
                     /locus_tag="PLU36_10020"
     CDS             complement(441..677)
                     /locus_tag="PLU36_10020"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HMZ47129.1"
                     /translation="MEENEKIYILKKKKYIYGPYNFETLKQNNLKAGDMIWYEGLKDW
                     VPVEEIEGLASFIISDNTNNNGSKKSFFKKILGL"
     gene            complement(729..2318)
                     /locus_tag="PLU36_10025"
     CDS             complement(729..2318)
                     /locus_tag="PLU36_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HMZ47130.1"
                     /translation="MDNNKKQIWLPLFFAIVMIFGMLVGYNLHKHTTSSNFFSSGKTN
                     SIQEVVSLIQNKYVDKIKIDSISEATINDLLSHLDPHSIYIPQNDVQAINDDLIGNFY
                     GIGVEFQMFEDTVTVISVVKNGPSEKAGVQAGDKIIFVNDTTKIAGVKITTDVIRNLL
                     RGEENSKVNITVIRDQKQQKITITRGSIPVSTIDASYMIEPQTGFIRLNKFGEKTYEE
                     FMEQLEKLQKEGMKNLILDLRGNTGGLLNEAVDIADEFLSNNKLVVYTEGAKSARYEF
                     KCKRDGLFEEGKLIILVDETSASASEVLAGALQDWDRATIIGRRTFGKGLVQQQFSLS
                     NGGALRLTVSRYYTPLGRNIQKSYTNKSKEEYRRDALGIEPHEKYENEDSAHPKPSFK
                     TPSGKTVFGGGGILPDILVTTDSITLTNNIAKIYHTNFLNKVALKLYIQNKNYLSKIN
                     SADKFYAEKLPTENDLSLVEKIAKSDSISLQKFSEKEKETLLFSIKKLIAKQMFGNNG
                     YYQLANKTDKVVLKAMEQVKQ"
     gene            2506..>2698
                     /locus_tag="PLU36_10030"
     CDS             2506..>2698
                     /locus_tag="PLU36_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HMZ47131.1"
                     /translation="MATLIADCGATKCDWSLLENNKVKKIVTQGFSLYFLSAENVKQL
                     LITELLSKIKNTTVLKVYFY"
BASE COUNT          883 a          444 c          319 g         1052 t
ORIGIN      
        1 gaatatcatc tattgggtca attggtccac ctggatcatc atcatctgca tcaacagaag
       61 ttgtaccagc aaaaccacca ccattagcag cagtattacc agtagaagtt gagttatctt
      121 caaaaacagg ttcgtctatt ccactattag tgcttgtaga accagttcca tttagtgtat
      181 tgccattatg gtcaaaaatt tgggaaaaaa cactattgta acttcctaag ttaataaaag
      241 ctccaaataa taaaataaat aataaatact gtttgcttct tttcatgctt taaatttatt
      301 ccaagtcaaa aaatcttaat tataggttta ggtgattact tagctgacaa aattataata
      361 attatttata cattatacaa tttggtctaa ataaatttcc atattcttcc ttaaacctga
      421 tttatttgaa aaaatataga ttataatcct aatattttct taaaaaacga ctttttagat
      481 ccattattat ttgtattatc agaaataata aatgatgcta aaccttctat ttcttctaca
      541 ggtacccaat cttttaagcc ttcataccaa atcatatcac ctgcctttaa attattttgt
      601 tttaaggttt caaaattgta gggcccatat atatactttt tctttttaag aatgtatatc
      661 ttttcatttt cctccataga atagttctta gatactgaaa tttataaacc tgctgtatga
      721 atataaaatt attgtttaac ctgttccatt gcctttaaaa ctactttatc agttttgttg
      781 gcaagttgat aatacccatt atttccaaac atttgtttag caattagttt tttaatgctg
      841 aaaagtaagg tttccttttc tttttctgaa aatttttgta agctaatgga gtcagatttt
      901 gcaatttttt caaccaaaga taaatcattt tcggttggta atttttcagc ataaaactta
      961 tcggcagaat taattttact taaataattt ttgttttgaa tatataactt aagagcaacc
     1021 ttatttaaaa agttagtatg ataaatttta gcaatattgt tggttaatgt aatagagtct
     1081 gtagtaacta atatatcagg taaaattcca cctccaccaa aaactgtttt accactaggt
     1141 gttttaaatg aaggtttagg gtgtgccgaa tcttcatttt catatttttc gtgtggttca
     1201 atacctaaag catctctcct atattcctct tttgatttat tggtataaga tttttgaata
     1261 tttctgccta aaggtgtata atacctagat actgttaatc ttaatgctcc cccattgctt
     1321 aaagaaaatt gttgctgcac caatcctttt ccaaaggttc ttcttcctat aatagttgct
     1381 ctatcccaat cttgtaaggc accagctaaa acctcactag cacttgcact tgtttcatcc
     1441 actaaaataa ttaacttacc ttcttcaaac aaaccatctc ttttacactt aaattcatac
     1501 ctggcagatt ttgctccttc ggtatataca actaatttat tgttcgataa aaattcatct
     1561 gcaatatcta cagcttcatt tagtaatcca cctgtatttc ctcttaaatc aagaatcagg
     1621 tttttcattc cttctttctg caatttttct aattgctcca taaattcctc ataggttttt
     1681 tcaccaaatt tatttaatct aataaaacct gtttgtggtt ctatcatgta agatgcatct
     1741 atagtgctta caggaattga gcctcttgta atagttattt tttgttgttt ctgatctctt
     1801 attactgtaa tatttacttt gctgttttct tctcctctta ataaatttct tattacatct
     1861 gtggttattt ttacgcctgc aattttagta gtatcattta caaaaataat tttatctcca
     1921 gcctgtaccc ctgctttttc tgaaggcccg tttttaacta cagaaattac tgtaacagta
     1981 tcttcaaaca tttgaaattc tacaccaatg ccataaaaat ttccaattaa atcatcatta
     2041 attgcttgta catcattttg gggtatataa atagagtgtg ggtctaaatg agatagtaaa
     2101 tcatttatgg tagcttcaga aatgctatct atttttattt tatcaacata tttattttga
     2161 attaagctaa ctacctcttg tatggaattt gttttacccg aactaaaaaa attactgcta
     2221 gtagtatgtt tatgtaaatt ataaccaact aacattccaa atatcattac aatagcaaaa
     2281 aatagaggta gccaaatttg tttcttatta ttatccatca aaatttgata ctttaggtga
     2341 gtacttttta ttaaaacacg aatatcagta tatcttcaaa acttgcctaa attcttttta
     2401 gttcaaccaa aaaatgtttt tattccttaa caaaattcct taaattgaat tatatttcat
     2461 atttaaccga aactttcttt tactgtaatt tacaaaaata aaattatggc tacattaatt
     2521 gcagactgtg gtgcaacaaa atgcgattgg agtttgcttg aaaacaacaa agtaaaaaaa
     2581 atagttaccc aaggttttag tttatatttt ttaagtgccg aaaatgtaaa acaactttta
     2641 ataaccgaat tactaagtaa aattaaaaat actactgttt taaaagttta tttttatg
//
LOCUS       DAOQPK010000271         2689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_30309_length_2689_cov_4.712604, whole genome shotgun sequence.
ACCESSION   DAOQPK010000271 DAOQPK010000000
VERSION     DAOQPK010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30309_length_2689_cov_4.712604"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="PLU36_10035"
     CDS             complement(<1..580)
                     /locus_tag="PLU36_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HMZ47132.1"
                     /translation="MPNHHPHHSLSDVHETVDTTQPRKGIKRFLAYVGPAYLVSVGYM
                     DPGNWATDLQGGAKFGYQLIWVLLMSNLMALLLQSLSARLGIVRRRDLAQANRETYPP
                     LINFCLYILAEIAIAACDLAEVLGMALGIYLLTGLPLIWGVSITVLDTFLLLWLQRFG
                     IRKLEAFIIALVIVIGLSFLIQIFLAKPNATEL"
     gene            757..1344
                     /locus_tag="PLU36_10040"
     CDS             757..1344
                     /locus_tag="PLU36_10040"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ47133.1"
                     /translation="MKSLLILFISVFLWNNIVKAQPPCINGHINTDLSASLSAGLYYI
                     DTDVYVTSDITITDAELLVIGNVVFLSQNLYNAITNEHRIFTTSCEIRSTEFPDSFVT
                     NNYVAKPANKIIENNTLSVYPNPVNNNVTVKGNDIAYIEVYTIYGIKAYSSQHYNYKG
                     AAVINLPLKNLAQGMYILKVYDRQNKSLSRKLIKQ"
     gene            1346..>2689
                     /locus_tag="PLU36_10045"
     CDS             1346..>2689
                     /locus_tag="PLU36_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47134.1"
                     /translation="MKYWVTILLVLQGYIGNTQYNLVPNYSFEDSVVCPSDINGGPLP
                     APWYLPQNVPNKLCYFHSCSTSSNYSVPNNVGNTSFQYAKTGQGYAGVDVYNSLSFFD
                     GRYYLQTKLKSLLQIGGCYYAEFWVSLLNYSRIATNNIAMLVTDTAILVDASYDAQFG
                     IGLIPANPQIFNYGNPVITDTLNWVKISGVFTAQGGEEYLTIGNFKDDTNTDTLRVNS
                     GVNTYEKAAYYIDDIYLIPLDSMFLHADAGTDKTITQGDSVYIGSYINGINNITWYNS
                     TGSIIATGIPGMYVQPAASTFYVIEQTVCGQYSKDTVYITVGVVPLVIKNYELIIKNE
                     GVVNKWITLNEINVSHLNVQRSSNGVEFNTIQQTAAKNNTYNEYSITDEQPLNGTSYY
                     RIEAVDKDGKRTYTKTEKVQSKIENEKLKIYPNPAKDIVNVSFPKIKQVTVADIIG"
BASE COUNT          983 a          420 c          444 g          842 t
ORIGIN      
        1 ctaactctgt tgcatttggt tttgccaaaa atatttgaat taaaaaagac aagccaataa
       61 ctattactaa agctataata aaagcttcta attttcttat tccaaaacgt tgtagccata
      121 gtagtaaaaa agtatctaat acagtaatac ttacccccca aattaaaggc aagcctgtaa
      181 gtaaatatat acctaatgcc atacctagta cttcggctaa atcgcaagca gcaatagcaa
      241 tttcggctaa aatgtataaa cagaaattga ttagtggagg ataagtttct ctattagcct
      301 gggccaaatc tcttcttcta acaataccca acctagcact taaactttga agtaataatg
      361 ccattaaatt gctcattaat aaaacccaaa ttagttggta tccaaatttg gctcctcctt
      421 gcaaatctgt tgcccaattt cctggatcca tataacctac gctaaccaaa tacgctgggc
      481 ctacataggc taaaaaacgt ttaatacctt ttctgggttg tgtagtatct acagtttcat
      541 gtacatcgct taaagaatga tgaggatgat ggttgggcat atatacaact aaaataaaac
      601 tttaaattta attattagtt taaactattt tatactatat acaactagtt aaaaaaaata
      661 aaattactac actttaagcc actccaatgc tgcaattttt attatgagtg cataaaatta
      721 aaacactaac tttaccctaa cataaaaata atgtttatga aatcgctgct cattcttttt
      781 ataagcgttt ttctatggaa taatattgtt aaggcacaac cgccttgcat taatggtcat
      841 attaatacag atttaagtgc ttctctttct gctggtcttt attatataga tacagatgtg
      901 tatgttacaa gcgatattac tataaccgat gcagaattat tagtaattgg caacgtggta
      961 tttttaagcc aaaacctgta caatgccatt accaatgagc atagaatatt tactacaagc
     1021 tgcgagatca gaagtaccga gtttcctgat agctttgtta ctaataacta tgtagctaaa
     1081 cctgccaata agattataga aaataatacc ctaagtgttt accccaaccc cgtcaataat
     1141 aatgttacag taaaaggaaa tgatattgct tatatagaag tatatactat ttatggtata
     1201 aaagcatata gctctcaaca ctacaattat aaaggagcag cagttattaa cctaccatta
     1261 aaaaatttgg cacagggaat gtatatacta aaagtatatg ataggcaaaa caaaagttta
     1321 agtcgtaagc tgataaagca ataaaatgaa atattgggta acgatattat tagttttaca
     1381 aggttatatt ggcaataccc aatataacct tgtacctaat tacagctttg aggatagtgt
     1441 tgtatgtcct agtgatatta atggtggacc attgcccgcc ccttggtact taccacaaaa
     1501 tgtaccaaat aaattatgtt attttcattc atgttccact tcatcaaatt atagtgtgcc
     1561 gaataatgtt ggcaatacca gttttcaata tgctaaaaca ggacaaggtt atgcaggagt
     1621 ggatgtatat aatagtcttt ccttttttga tggtagatat tatcttcaaa caaagcttaa
     1681 aagtctatta cagatcggtg ggtgttatta tgctgagttc tgggtttctc ttttaaatta
     1741 ttcaagaatt gctaccaata atatagctat gctcgttaca gatacagcaa tattggtaga
     1801 tgccagttat gatgctcaat ttggtattgg tttaatacct gctaatccac aaatatttaa
     1861 ttatggtaat cctgtaatta cagataccct gaactgggta aaaattagtg gtgtatttac
     1921 ggctcagggt ggggaggaat atttgactat tggtaatttt aaagatgata ctaatacaga
     1981 cactctaaga gttaattcag gagtaaatac ttatgaaaaa gcagcctatt acatagatga
     2041 tatatattta atacccttag acagtatgtt tttacatgca gatgcaggta cagataaaac
     2101 cataacacag ggggatagtg tatatatagg cagctatata aatggtatta ataatattac
     2161 atggtacaac agcacaggca gcataatagc aacaggcata cccggtatgt atgtacaacc
     2221 tgctgcaagt actttttatg taatagagca aaccgtgtgt gggcaatact ccaaagacac
     2281 tgtatatatt actgtaggtg tagtaccgtt agtaattaag aattatgaat taataattaa
     2341 gaatgaagga gtagtaaata aatggattac tttgaatgaa ataaatgttt cgcatttgaa
     2401 tgtacaacgc agcagtaatg gtgtagaatt taatactata caacaaacag cagcaaagaa
     2461 taatacttat aatgaataca gcattacaga tgagcaacct ttaaatggaa cgagttatta
     2521 tcgtatagag gcagtagata aagatgggaa aaggacttat actaaaacag agaaagtgca
     2581 atcgaaaatt gagaatgaaa aattgaaaat ttatccaaat cctgcgaaag atattgtaaa
     2641 tgttagtttt cccaaaataa aacaagtaac tgttgcagac ataatagga
//
LOCUS       DAOQPK010000272         2682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_30444_length_2682_cov_3.689760, whole genome shotgun sequence.
ACCESSION   DAOQPK010000272 DAOQPK010000000
VERSION     DAOQPK010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30444_length_2682_cov_3.689760"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="PLU36_10050"
     CDS             <1..877
                     /locus_tag="PLU36_10050"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ47135.1"
                     /translation="LMFNEAAFIYCYLMFYIENKKDEALEFIKSKKLDVVNNHLFSYM
                     AANLSLNNKQINNAAIILQNRNKSGDYLNTPVWDFQMGFVCLYRMQTQQAINYFENYL
                     SQFKGNFYVKDIYQKLSWCYYLQGNMAKAEDARKKCINRGTQVTEADKQAYKDAKKNK
                     WANIVLLKARILNDGGFNNEAIKVLHGFSTSSFKDEAEQLEFNYRVGRINDDLGKEED
                     AIAFYLKAITIGEHRTEYYAARAALQIGMIHEKKGNKKLAITYYEKCMDMENHDYKNS
                     LDQRAKSGIARCYGN"
     gene            928..2580
                     /gene="recN"
                     /locus_tag="PLU36_10055"
     CDS             928..2580
                     /gene="recN"
                     /locus_tag="PLU36_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HMZ47136.1"
                     /translation="MLSKLHIQNYAIIDEIEINFFNELNIITGETGAGKSIIMGALNL
                     ILGERADSTYLLNQNKKCFIEGLFITEKKEEIVNFLRTNDFDILDELVLRREIATNGK
                     SRAFINDSPATLIQLKQLAALLVDLHQQFDTLELSNSHFQRNVVDALANNAQILSNYQ
                     RLFLQWQSNQKKLEELILQRNNFSKEADYNKYLLQELEEINLKENEIEGIENELEILN
                     NSTEIKTALNALYFELKDNETPIIVKLKQLSSQLNSYAAYHQNIQSITDRIQTLQIEL
                     DDIANEAATLNNAINLDENKIEILNEKLSLCYKLYKKHNVQSSAGLIAIQNELEEKLK
                     AVLNIDEVIKEKEKEAKDLLSKISIMAKEISASRNAQLIPIEKKVNSLLHQIGMPNAN
                     IKIELKEVPLNYYGKDNVEFLFDANKSNKFEPINKVASGGELSRLMLCIKSLVAQSID
                     LPTLIFDEIDSGISGEAAKQVGIIMQQLAAKRQIICITHQPQIAGKANAHFYVYKEIK
                     NDIVKTNIKLLNNDERIINIAKMLSGEKPSTAAIESAKEMMN"
BASE COUNT         1119 a          336 c          422 g          805 t
ORIGIN      
        1 attaatgttt aacgaagctg cttttattta ctgttattta atgttttata tagaaaataa
       61 aaaggatgaa gctttagagt ttattaaaag taaaaaatta gatgttgtaa ataatcactt
      121 gtttagctac atggctgcta acctaagttt aaataataaa caaataaata atgccgccat
      181 tattttacaa aacagaaata aatcgggaga ttatttaaat actccagtat gggattttca
      241 gatgggattt gtatgtttat acagaatgca aacccaacag gcaattaatt attttgagaa
      301 ctatttatct caatttaaag gaaattttta tgttaaagat atttaccaaa aactaagttg
      361 gtgctactat ttacaaggca atatggctaa agcagaagat gccagaaaaa aatgtatcaa
      421 tagaggaaca caagttacag aggctgacaa acaagcctat aaagatgcca agaaaaacaa
      481 atgggcaaac atagtattac ttaaagccag aatattaaat gatggtggtt ttaataacga
      541 ggcaataaaa gttttacatg gctttagtac ttcaagtttt aaagacgaag cggaacaatt
      601 agagtttaat tatagagttg gcagaataaa tgacgactta ggtaaagagg aagatgctat
      661 tgctttttat ttaaaagcta ttacaattgg agaacataga actgaatatt acgctgctag
      721 ggctgcttta caaattggta tgatacatga gaaaaaaggt aataaaaaat tagctatcac
      781 ctattacgaa aaatgtatgg atatggagaa ccatgattat aaaaacagct tagaccaaag
      841 ggcaaaaagt ggcattgcac gttgttatgg aaattaaagt ttcctcactt aatcatcatt
      901 aataataaat atcttttaac tttaaatatg ctctcaaaat tacatattca gaactatgca
      961 atcatagacg aaattgaaat taatttcttc aatgaactaa atattattac aggagaaact
     1021 ggtgctggca aaagtataat tatgggtgct ttaaatttaa ttttaggaga aagagcagac
     1081 agtacttact tattaaacca aaataaaaaa tgttttattg aaggcctttt tataacagaa
     1141 aaaaaagaag agatagttaa ttttttaaga actaacgact ttgatatttt agatgagtta
     1201 gtattaagaa gagaaatagc aaccaacgga aagagtagag cttttattaa cgactcacct
     1261 gctacactta ttcaattaaa gcagctcgca gctttattgg ttgatttaca ccaacaattt
     1321 gatacattag aattaagtaa cagccacttt caaagaaatg tggttgatgc tttggcaaat
     1381 aatgctcaaa tattatccaa ttaccaacgc ctttttttgc aatggcaaag caatcaaaaa
     1441 aaattagaag aacttatatt gcaaagaaat aatttcagta aagaggcaga ttataacaaa
     1501 tatttattgc aagaattaga agaaatcaat ttaaaagaaa atgaaattga aggtatagaa
     1561 aatgaacttg aaattttaaa taacagtacc gaaataaaaa ctgctttaaa tgcactttat
     1621 tttgagctaa aagataacga aactccaatt atagtaaaac ttaaacagct ttcatctcaa
     1681 ctaaactctt atgctgcata ccatcaaaat attcaatcta taacagatag aatacaaaca
     1741 ttgcaaattg agttagatga tattgctaat gaagctgcaa cacttaataa tgctattaat
     1801 ttagatgaaa ataaaattga aattttaaat gaaaaacttt cgctttgtta taaactatac
     1861 aaaaaacata atgtacaatc atcagcaggt ttaattgcta ttcaaaacga attagaagaa
     1921 aaattaaaag cagttttaaa tattgatgaa gtaataaaag aaaaagaaaa agaggctaaa
     1981 gatttactat ctaaaatttc aataatggca aaagagattt ctgcttcaag aaatgctcaa
     2041 ttaataccta ttgaaaagaa agtaaattct ttgttacatc aaattggtat gccaaatgca
     2101 aacattaaaa tagaattaaa agaagtaccg ttaaattatt acggaaaaga taatgttgag
     2161 tttttatttg acgctaataa aagcaacaaa tttgagccaa ttaataaagt tgcaagtggt
     2221 ggagagttaa gcaggttaat gctttgcata aaaagcttag tagcacaaag tatagattta
     2281 cctactttaa tttttgatga aattgactct ggaattagcg gtgaagctgc aaaacaagtt
     2341 ggaattatta tgcaacaact agcagctaaa aggcaaataa tttgtattac acatcagccc
     2401 caaattgctg gtaaggcaaa tgctcatttt tatgtttata aagaaattaa aaatgatata
     2461 gtaaaaacca atattaaatt attaaacaat gatgaacgca ttataaacat tgccaaaatg
     2521 cttagtggag aaaagccaag tactgcagca atagaaagtg caaaagaaat gatgaactag
     2581 ttttggttga aaataagtac attttacgaa caacaaatta gtaaaactac gaagcctaaa
     2641 agtatatttt ccttgctcat tttcagtaaa ctgaatgtct aa
//
LOCUS       DAOQPK010000273         2678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_30530_length_2678_cov_4.948151, whole genome shotgun sequence.
ACCESSION   DAOQPK010000273 DAOQPK010000000
VERSION     DAOQPK010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30530_length_2678_cov_4.948151"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..1301)
                     /locus_tag="PLU36_10060"
     CDS             complement(60..1301)
                     /locus_tag="PLU36_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HMZ47137.1"
                     /translation="MIHFNKHTLHNGLKIIVHEDNSTPMAVVNIMYNVGARDEDPQKT
                     GFAHLFEHLMFGGSINIPEYDEPLQKAGGDNNAYTTNDLTNYYCVLPAQNIETAFWLE
                     SDRMLSLAFNKKSLNVQRKVVCEEFKEHYINKPYGDIWHNMRNLAFDVHPYKWMTIGK
                     ELSHIENATIEDVKAFFFKHYRPNNAIVVVAGNVQSNQIFQLVEKWFGNIPSSENSIK
                     NIPKEPQQNNAKFLELQANVPLDAIIKTWHIDARLTKGYYAADLITEIFGGGASSRLY
                     NSLVKNKKLFSSINCYHFGSIDPGLLTIEGKLIKGIKMQEAENAIEEELEKLKSTLIE
                     NNELQKVKNRTESMITFEDISVESRANSLAMYELLGDAAWMNTELEKYQEITIHDIQQ
                     YAQTIFNTKNSSTIHYYSKNN"
     gene            complement(1362..>2678)
                     /gene="dnaB"
                     /locus_tag="PLU36_10065"
     CDS             complement(1362..>2678)
                     /gene="dnaB"
                     /locus_tag="PLU36_10065"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HMZ47138.1"
                     /translation="ILTVVEELKRKELLQQVGGAYYITKLTNTVVSTANIDAHARIIL
                     QKFIQRELIRISGEIIGDAYEDSTDVFDLLDDSETKMFNITNNYLKKNFEDIGSVLTT
                     TINRIDYLRTREDDISGVPSGFSNIDSITFGWQPTDLLILAARPGVGKTAFALNLARN
                     AALHPIKPTAVGFFSLEMSASQLVQRILSAESEIPLQKISRGRLEEHEYQQLHTKGIK
                     RLEQAPLFIDDTAALNIFEFRAKARRLVNNHKVGLIIIDYLQLMSGSSENRNTNREQE
                     ISTISRNLKALAKELSVPIIALSQLSRAVESRKESKVPQLSDLRESGAIEQDADMVMF
                     IYRPGYYEITANENGENIKDLAELKIAKHRNGSLETLQLKMIPEIQKFEVWDRGDSGL
                     GGNYKSLGPSPNNPSDSQNGGKFLYQTSKMNNGNFDDGYDNEQPID"
BASE COUNT          763 a          456 c          393 g         1066 t
ORIGIN      
        1 ctggtgcctg ttttctgaaa gacattgttg taattaaaat ttaaaaacta taatataaat
       61 taattgtttt tgctgtagta atgtatagta ctactatttt ttgtattaaa aattgtttga
      121 gcatattgtt gaatatcatg aatagtaatt tcttgatatt tctctaattc ggtattcatc
      181 catgctgcat ctcctaaaag ttcgtacata gctaagctat ttgccctgct ttccacactt
      241 atatcttcaa atgttatcat actttctgtt ctgtttttta ctttttgtag ctcattgttt
      301 tcaattaaag tagattttaa tttttcaagt tcttcttcaa ttgcattttc tgcttcttgc
      361 attttaatgc ccttaattaa cttaccttct atagttagta aacctggatc tatagaacca
      421 aaatggtagc aattaataga ggagaaaagt tttttatttt ttactaaact gttgtacagc
      481 cttgaggatg caccacctcc aaatatttcg gttatcaaat ctgcagcata ataacccttg
      541 gttaaacgag catctatatg ccatgttttt ataattgcat ctaaaggaac attggcttgc
      601 aattctaaaa attttgcatt gttctgttgg ggttctttag gtatattttt aatgctattt
      661 tctgaggaag gaatattgcc aaaccatttt tctactaatt gaaaaatttg gttgctttgc
      721 acattgcctg ccactactac aattgcatta ttaggacgat aatgtttaaa gaaaaatgct
      781 ttcacatcct ctatagttgc attttcaata tggcttaatt ctttacctat cgtcatccat
      841 ttataaggat gtacatcaaa ggcaaggttt ctcatattat gccaaatatc gccataaggt
      901 ttgtttatat aatgttcttt aaactcttcg caaacaactt tacgttgtac atttaaactt
      961 tttttattaa aagccaaact cagcattcta tcactctcta accaaaatgc agtttcaata
     1021 ttttgtgctg gcaatacaca gtaataattt gttaaatcgt ttgttgtata agcattgttg
     1081 tctccaccag ctttttgtag aggctcatca tattctggaa tatttatact tccgccaaac
     1141 attaaatgct caaataaatg tgcaaagcct gttttttgcg ggtcttcgtc tcttgcacca
     1201 acattgtaca taatatttac aacagccatt ggggtgctgt tatcttcgtg tacaattatt
     1261 tttagtccat tatgtagagt atgtttatta aaatgaatca ttaaacttta gtaaaagttg
     1321 aaagaatata gttatagaat aagagttgaa ttaaataaat tctagtcaat tggttgttca
     1381 ttatcgtagc catcatcaaa attgccatta ttcattttgc ttgtttggta taaaaacttt
     1441 cctccatttt gtgaatctga agggttattt ggcgatggtc ctaaagattt ataatttcct
     1501 cctaaaccgc tgtcgcctct gtcccaaact tcaaattttt gaatttctgg aatcattttt
     1561 agctgaagag tttctaagga gccatttcta tgttttgcaa tttttaattc ggctaaatct
     1621 ttaatattct ctccattttc gttggctgtt atttcgtaat agcctggacg ataaatgaac
     1681 attaccatat ctgcatcttg ttctattgca ccagactcac gtaaatcgct taattgtggt
     1741 actttacttt ctttacgaga ttctactgca cggcttaact gagataatgc aataattggt
     1801 acacttagtt cttttgctag tgcttttaaa tttctggata tggtagaaat ttcttgctct
     1861 ctgtttgtat ttctattttc agaggagcca ctcattaatt gtaagtagtc tattataatt
     1921 aaacctactt tatgattatt tactaatctt cttgcttttg ctctaaattc aaaaatattt
     1981 agggcggctg tatcatcaat aaataaagga gcttgctcta atctttttat accttttgta
     2041 tgtagttgtt ggtattcgtg ttcttctaat cggcctcttg aaattttttg gagtggtatt
     2101 tcactttctg cacttaatat acgttgtact aattggctgg cactcatttc taaactaaaa
     2161 aacccaacag cagttggctt gattggatga agtgctgcat tgcgtgcaag gtttaacgca
     2221 aaagcagttt tacccacacc tggccttgca gcaagtatta ataaatcggt tggctgccat
     2281 ccaaaggtta tactatctat attagaaaag ccacttggta cgccactaat atcatcttct
     2341 ctggttctta aataatctat tctatttatt gtggttgtta aaacgcttcc aatatcttca
     2401 aaatttttct ttaaatagtt attggttatg ttaaacattt tggtttcgct gtcatctaat
     2461 aaatcaaaca catctgtact gtcttcataa gcatcgccaa ttatttcgcc acttattctt
     2521 attaattctc tttgaataaa tttttgtaaa attattcttg catgagcatc aatatttgcg
     2581 gtacttacaa ctgtattggt taatttggtt atataatatg caccgccaac ttgttgtaat
     2641 aattcttttc ttttaagttc ttctactact gttaaaat
//
LOCUS       DAOQPK010000274         2664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_30818_length_2664_cov_3.784209, whole genome shotgun sequence.
ACCESSION   DAOQPK010000274 DAOQPK010000000
VERSION     DAOQPK010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30818_length_2664_cov_3.784209"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(272..643)
                     /locus_tag="PLU36_10070"
     CDS             complement(272..643)
                     /locus_tag="PLU36_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47139.1"
                     /translation="MKQRYQINKEAKVGCECTCPSCGTIFIKTNYQQVFCKSKTGTIC
                     KDKYWNTVDPKKRCNRTRISPASATYMANQIEIRGTVKKHFTTEGYEIIRGVAYDEFG
                     DPVYNVDPFDDTHPFSEDAFN"
     gene            795..971
                     /locus_tag="PLU36_10075"
     CDS             795..971
                     /locus_tag="PLU36_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47140.1"
                     /translation="MNWRNKGLYNGTPNFGWDIDHIIPLSSAVNETDIIKLNHYTNLR
                     PLCSYVNRYVKRDK"
     gene            complement(982..1548)
                     /locus_tag="PLU36_10080"
     CDS             complement(982..1548)
                     /locus_tag="PLU36_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47141.1"
                     /translation="METTISTKALQVIKAVKEATSKSITGVSFVSVKNYTNSKGEVSN
                     NLINIGIKYESAKKKDIAFLNELNIEKAVKEFGLKSEISLLEQARKDLMVSFIKPEVN
                     RSNGQKDAYTVIFEGVKVHNETGVLYIYGYREKKDVLVKGVYPIVNSKPLTNAKDELR
                     KLLKTGKFTQYALEVGNTLKLNGERLEL"
     gene            complement(1642..1764)
                     /locus_tag="PLU36_10085"
     CDS             complement(1642..1764)
                     /locus_tag="PLU36_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47142.1"
                     /translation="MNRKISRDIELENNLRINHHKVHKSKKAYNRKESKKINWD"
     gene            1971..2261
                     /locus_tag="PLU36_10090"
     CDS             1971..2261
                     /locus_tag="PLU36_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47143.1"
                     /translation="MTPFETAQQLAAEGKFATPRVSYGAKGVDYFGYSLATHHFNLKL
                     MAKGMTFKGIKFTDIKNYYGLTGRSAKDCLPQFEKIMADYKEKLAKEQKTTE"
BASE COUNT          782 a          405 c          461 g         1016 t
ORIGIN      
        1 aaaaaaaatt aaaaaagggg taggtctact ttttaggtca aaaatttccc aaaaaattta
       61 tttacatctt catagcccct ataagagggg gtagacgggg gggctacaaa ggggggtaac
      121 ggagggaggg ctcccgtagg ggaggggtcg gtgcccctct taatgtttgt gtgctgtaaa
      181 gataagatta ttttttcatg tgtgcaagtt ttttagttag aagttattaa caaagttatg
      241 aacaagggct ttcgcccttg cttttatagt tctaattaaa tgcgtcttca ctaaatggat
      301 gtgtatcatc aaaggggtcg acattataaa cagggtcgcc aaactcatca taagctactc
      361 cgcgtatgat ttcatacccc tcagttgtaa agtgtttttt aactgttcct cttatttcaa
      421 tttggttagc catatatgtt gcacttgctg ggcttattcg tgttctatta cagcgttttt
      481 tggggtcaac agtgttccaa tatttatctt tacaaattgt acctgtttta gacttacaaa
      541 aaacttgttg atagttagtt tttataaaga ttgtcccaca acttgggcaa gtacattcac
      601 accctacttt agcttcttta ttaatttggt atctttgttt cattttcata ctgcaaatat
      661 aggtataata ttttaaacca ccaaatttat tttgttaaat tattgttcaa tggttgacac
      721 ttttattaaa atttcgtata tttatagata ttgaaacatt taaactacat ttagaaaacc
      781 agttttaatc ttggatgaat tggcgaaata aaggattgta taatggtaca cctaattttg
      841 gatgggatat agaccatatt ataccgttaa gtagtgcagt taatgaaact gatataataa
      901 agttaaacca ttatactaat ttacgtccgt tatgtagtta cgttaatagg tatgtgaaaa
      961 gggataagta atccctttcg gctacaactc taatctttct ccgttcaatt ttaaagtgtt
     1021 tccaacttct aaagcgtatt gagtaaattt acccgttttt aaaagctttc ttaattcgtc
     1081 ttttgcattt gttaaaggtt tgctgtttac aataggataa acacccttaa ctaaaacgtc
     1141 ttttttctct ctgtaaccat agatataaag cacgcctgtt tcattgtgta cctttacacc
     1201 ctcaaagata accgtataag cgtctttttg accattagag cgattaactt cgggcttaat
     1261 aaagcttacc attaaatctt tgcgagcttg ttctaaaagg cttatttcgc tttttaaacc
     1321 aaattcctta actgcttttt caatgtttaa ttcgttcaaa aaagcaatat cttttttctt
     1381 tgcactttcg tacttaatac ctatgtttat aaggttatta gatacttcgc cttttgagtt
     1441 agtgtagttt ttaacactta caaaagatac gcctgtaatg cttttagatg tagcctcttt
     1501 tactgcttta ataacttgta atgcttttgt tgagattgta gtttccattg ttttattgtt
     1561 taattgtgtg gtacaaatat aaggacttta ttttaatcta ccaaatttat tttgttaaat
     1621 tttaaagaaa gtttgggatt gttaatccca attaattttt ttactttctt tgcggttgta
     1681 tgcttttttg cttttatgta ctttgtggtg gttaattcgc aagttatttt ctaattcaat
     1741 atccctgcta attttacggt tcataaccat tatttgttcg gtggttattt tcatgttttt
     1801 atttgacttt gccattggta gttgttttta attgttatag ggcaaagata atactttttt
     1861 ttatatctgc aaactttttt taaaaataat cctggtattt ttcctggttt ttatttggtg
     1921 gtttaaaata atatttgtat atttgccgta tcaattaaaa cattataacc atgacaccat
     1981 ttgaaaccgc acaacaatta gcagctgaag gtaaatttgc cacgcctcgt gtatcttatg
     2041 gggcaaaagg ggttgattat tttggttact cattagctac tcaccacttt aacctaaagt
     2101 taatggcaaa gggtatgacc tttaaaggta ttaagtttac cgacattaag aactattacg
     2161 ggttgactgg tcgcagtgca aaggattgtt taccacagtt tgagaagata atggctgact
     2221 acaaagaaaa gttagcaaaa gagcaaaaaa ctaccgaata aatttggtag tttcaaagta
     2281 aatgcttatc tttgtcctgt cagtgagacg ttcaagtgct gggaagcacg gggaagtata
     2341 ctccgacctg cattatattt tttttaggct attgcgacat ttattgcgac atggtgtcgc
     2401 aataggtgtc gggatgtaca caattgtgta ctaagttgtt ctaatacgca caccaataaa
     2461 tggtacttca atccaaatac tacggttaaa gcgttgccaa aaaaatggtt ttttgttgtc
     2521 gataaaaggg atgtttctgt aaaattctag tttcatggtt atttgttttt ggtatggtgc
     2581 aaagatagta aaaaatttta atttaccaaa aataattgta cttttgttaa taactttggt
     2641 tctttatacc ctttaaaata gatt
//
LOCUS       DAOQPK010000275         2642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_31287_length_2642_cov_3.425203, whole genome shotgun sequence.
ACCESSION   DAOQPK010000275 DAOQPK010000000
VERSION     DAOQPK010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31287_length_2642_cov_3.425203"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="PLU36_10095"
     CDS             complement(<1..910)
                     /locus_tag="PLU36_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HMZ47144.1"
                     /translation="MIAGILKEPNFENRVSLLPEQVQALIKKNVQVLVEEDAGKNASA
                     FNQAYINAGAEMVSRQKALTANLVLSIHPLTNNDLSCLNPNAVVLGVYQPLFNKDLMQ
                     SWASNNISCFSIDMIPRTTRAQSMDVLSSQANIAGYRAVLLAATLLPRYFPMFMTAAG
                     SIAPAKVLILGAGVAGLQAIATAKRLGAVVEVFDTRPAVKEEVQSLGAKFIEVEGAAD
                     ASKAGGYAVEQSDEYKQKQQHKIAESVAKSDVIITTAQIPGKQAPILITQAMLDTMKT
                     GSVIIDLAAATGGNTPKTKNNENFTTE"
     gene            complement(999..1796)
                     /locus_tag="PLU36_10100"
     CDS             complement(999..1796)
                     /locus_tag="PLU36_10100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ47145.1"
                     /translation="MYSPIIVALKYMQYYLKANNGKGHGAHSPFVYDFIINVLNDKRN
                     FYAYENIEKLREGLLKENSILHVEDFGAGSKIGLTKQRTVSAIASSSLKPAKYAQLLF
                     RMVNYYQPKTILELGTSLGITTAYLAAANNNAFVTTMEGSKEISSKATKNFELLELKN
                     IQQIQGNFDITLPALLPELSNIDFAFLDGNHRYLPTINYFNSILPKISNQTIVILDDI
                     HWSKEMEQAWNEIKNHESVTLSIDLFFIGILFFKSEFKVKQHFTIRF"
     gene            complement(1800..2324)
                     /locus_tag="PLU36_10105"
     CDS             complement(1800..2324)
                     /locus_tag="PLU36_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47146.1"
                     /translation="MLFEFIGTAYSCTILFLIIVYLFTKGRIKYLFKNFIAVSNTLLI
                     LYSTYLMYQFYQLVRYIYDLNIQLPKNASEKPIDISWFQIKFVLLIFLPFLFLSKQLK
                     SNIILAGIMLVLLQWDVAADMYQSIFMQSQTAGVIFYFPYLLFYKILNYLALFISVYA
                     LLWLAKKLPYQSLK"
     gene            complement(2325..>2642)
                     /locus_tag="PLU36_10110"
     CDS             complement(2325..>2642)
                     /locus_tag="PLU36_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47147.1"
                     /translation="KQGWKEGTFEFVIQQISLLESVKENLTKKVELKLDIQNLTKERI
                     DFITNNIIQNPGNATIKFRIKDEYEKMDLNLSHGTKGFTMNNDMAEFLLSNADVEVKV
                     NSN"
BASE COUNT          877 a          409 c          367 g          989 t
ORIGIN      
        1 tttctgttgt aaagttttca ttattttttg ttttaggtgt attaccacct gttgctgcag
       61 ctaaatctat aattacactt cctgtcttca tggtatctaa catggcttgt gtaattagta
      121 taggtgcttg tttgcctgga atttgtgcag tagtaataat tacatcgctt ttggctacac
      181 tttcggcaat tttatgttgt tgtttttgtt tgtattcatc cgattgttct acagcataac
      241 caccagcttt agaagcatct gctgcacctt ctacttctat aaatttagct cctaaacttt
      301 gcacttcttc tttaactgct ggccttgtat caaaaacttc tactacagct cctaagcgtt
      361 tggcagtagc tatggcttgt aaacctgcta cacctgcacc taatattaaa actttagcag
      421 gtgcaatact acctgcagct gtcataaaca taggaaaata tcgaggtaat aaagttgcag
      481 caagtaaaac tgctctatac cctgcaatat tagcttgact acttaataca tccatacttt
      541 gagctcttgt tgttctaggt atcatatcta tactaaaaca agaaatatta ttggatgccc
      601 agctttgcat taagtcttta ttaaataaag gttggtaaac accaagtact acagcattgg
      661 ggtttaagca acttaaatcg ttattggtta atggatgaat gcttaatact aaattggccg
      721 ttaaagcttt ttgcctgcta accatttctg cacctgcatt aatatatgcc tgattgaagg
      781 cactagcatt ttttcctgca tcttcttcta ccaatacctg tacatttttt tttattaagg
      841 cctgaacttg ttcgggtaat aaagaaactc tgttttcaaa attgggttct tttagtattc
      901 ctgctatcat tgttttggta ttaaaggcgt gaaaatataa attttaatga aaaaaggata
      961 ataatctatt taattgtttt tagttattgt aaaaaatatt aaaatctaat agtaaaatgt
     1021 tgctttacct taaattctga tttaaaaaat aatattccaa taaaaaataa atctatactt
     1081 aatgtaacag attcatgatt ttttatttca ttccatgctt gctccatttc tttgctccaa
     1141 tgaatatcat ctaaaattac aattgtttga ttagaaattt tgggtaaaat gctattaaaa
     1201 taatttatgg ttggcaagta tcggtgatta ccatctaaaa aagcaaaatc tatatttgat
     1261 aattctggca acagtgcagg taaagttata tcaaaattgc cttgtatttg ttgaatattt
     1321 ttcaattcta ataattcaaa attttttgta gcctttgaag aaatttcttt agaaccttcc
     1381 atagtggtaa caaaagcatt gttatttgca gcagctaaat atgctgttgt aatgcctaat
     1441 gaagtgccta actctagaat agttttgggc tgatagtagt taaccattct aaaaagcaac
     1501 tgagcatatt ttgcaggttt taaagaagat gaagcaatgg cacttacagt tcgttgtttg
     1561 gttaaaccaa ttttagaacc agccccaaaa tcttctacat gtaaaatact gttttctttt
     1621 aataaccctt cccttagttt ttcaatattc tcataggcat aaaaatttct tttatcgttt
     1681 aatacattta taataaaatc atatacaaaa ggcgaatgtg caccatgccc tttaccatta
     1741 ttagctttta agtaatactg catatatttt aaagcaacaa tgattggaga atacatattt
     1801 tattttaatg attgataagg taatttttta gcgagccaaa gtaaagcata aacactaatg
     1861 aatagtgcta aatagtttaa tattttataa aataacaagt aaggaaaata aaaaattaca
     1921 cctgctgttt gtgattgcat aaaaatagat tgatacatat ctgctgcaac atcccattgt
     1981 aacaaaacaa gcataatacc agccaaaata atatttgatt taagttgttt agataaaaac
     2041 aaaaatggta aaaatatcag caaaacaaac tttatttgga accaagaaat atctattggt
     2101 ttttcgctag catttttggg taattgaata tttaagtcgt aaatgtacct aactagttgg
     2161 taaaattgat acattaaata ggtgctatac aaaatgagta aagtattact aactgctata
     2221 aaatttttaa ataaatattt tattcttcct ttagtaaata aataaactat tattaaaaaa
     2281 agaatggtgc aggaataagc tgtgccaata aactcaaaca acatttaatt actatttact
     2341 ttaacttcca catctgcatt ggataataaa aattctgcca tgtcattatt catagtaaac
     2401 ccttttgttc catgcgataa atttaaatcc attttttcat actcatcttt tattctaaac
     2461 ttaatggttg cattgccggg gttttgaata atattatttg ttataaaatc tattctttct
     2521 ttggttaaat tttgaatgtc taactttaat tctacttttt tagtaaggtt ttcttttacc
     2581 gattctaata aacttatttg ttgaattaca aactcaaaag ttccttcttt ccatccttgt
     2641 tt
//
LOCUS       DAOQPK010000276         2638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_31369_length_2638_cov_4.668215, whole genome shotgun sequence.
ACCESSION   DAOQPK010000276 DAOQPK010000000
VERSION     DAOQPK010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31369_length_2638_cov_4.668215"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="PLU36_10115"
     CDS             <1..623
                     /locus_tag="PLU36_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HMZ47148.1"
                     /translation="KNDIVFAIGPAGTGKTYTAVALAVRALKNKVVKKIILTRPAVEA
                     GESLGFLPGDLKEKIDPYLRPLYDALDDMIPADKLGYYMTTRTIEIAPLAYMRGRTLD
                     NAFIILDESQNTNDLQLKMFLTRIGANAKAIITGDPTQIDLPRNQRSGLEKAVRILKN
                     IDGIAHIELDEEDVVRHKLVKAIIKAYDKEFEKEEAEYEKKYNHPR"
     gene            757..1158
                     /locus_tag="PLU36_10120"
     CDS             757..1158
                     /locus_tag="PLU36_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47149.1"
                     /translation="MKIFFSLIAFLLLACNNSSITYLPATRPLEAAREFIEAFKAGKF
                     EKASFYMLHDKENDKLLKEAKDKYYQFTKKDKRLLSEASIQNVTIETISTTETIIYYN
                     NSFDKERRKVKAVNQNNTWLIDFKYTFNPNL"
     gene            1158..1520
                     /gene="crcB"
                     /locus_tag="PLU36_10125"
     CDS             1158..1520
                     /gene="crcB"
                     /locus_tag="PLU36_10125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HMZ47150.1"
                     /translation="MLKNILLVGIGGGLGSIFRYFIGLMITNKHFPYATITVNVVGSF
                     FIGVIMAILAKQTHNVEHWYLIITTGFLGGFTTFSAFSWNTFQLMQQQQYAASFLYIG
                     LTFFLTLIAVSIGFFIFK"
     gene            1539..2087
                     /locus_tag="PLU36_10130"
     CDS             1539..2087
                     /locus_tag="PLU36_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HMZ47151.1"
                     /translation="MQQLVQHPWHGVNPGEQAPRKVNAVIEIPQGSRCKYEIDKPSGL
                     LKLDRIIYSSFYYPCNYGFIPQTYGGDKDPLDILVITSIPVVPLTLMEAKVVGVMQMI
                     DSGDADDKIIAVASNDPGVNHYNNIEELPKHFFDELRHFFEEYKKLENKEVKVEEFGD
                     KTTALKIVSEAIEFYKKSFLNK"
     gene            2095..2529
                     /locus_tag="PLU36_10135"
     CDS             2095..2529
                     /locus_tag="PLU36_10135"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HMZ47152.1"
                     /translation="MTVNTIIILIIIGVAAGMLSGLVGVGGGIIIVPALIYILGFSQK
                     TAQGTSLGLLLLPVFIVSLIYYYKAGEVNIKAIPFLAIGFLVGGYLGSKLALSLPDAT
                     VKKIFAFLMIIVAVKMLFFDKPKKDTNKIHATEAQKNKSVGS"
BASE COUNT          974 a          376 c          411 g          877 t
ORIGIN      
        1 ataaaaacga tattgttttt gcaataggac cagcaggtac tggtaaaaca tatacagcag
       61 ttgcattagc ggttagagca ttaaaaaata aagtagttaa aaaaattatt ttaaccaggc
      121 cagccgttga agctggagaa agcttaggtt ttttaccagg tgatttaaaa gaaaaaattg
      181 acccttattt aagaccatta tacgatgcat tagacgatat gatacctgcc gataaactag
      241 gttattatat gactaccaga actattgaaa ttgccccatt agcctatatg agaggcagaa
      301 ctttagataa tgcatttatt attttagacg aatcgcaaaa tactaacgac ttacaattaa
      361 aaatgttttt aacccgtatt ggtgccaatg caaaagctat tattactggc gaccctacac
      421 aaatagattt gccacgcaac caacgtagtg ggttagaaaa agctgttaga attttaaaaa
      481 atattgatgg tattgctcat attgaattag acgaagaaga tgtagtaaga cataaattag
      541 taaaagccat tattaaagca tacgataaag aatttgaaaa agaagaagct gaatacgaaa
      601 aaaaatataa ccatcctcgt taaaaaatta cttactaacc attcatataa attaggcggc
      661 tttaaaagct gccttttcta ttcaccaaaa tcaaccttac ctttgtttga atatcatgaa
      721 ctaggttttg tattttcaat ttacataaag ttatccatga agatattttt ttcacttatc
      781 gcctttttac ttttagcttg taacaattca agcattacat atttaccagc aaccaggcca
      841 ttagaagctg ccagagaatt tattgaagct tttaaagcag gtaaatttga gaaagcaagt
      901 ttttatatgc ttcacgataa ggaaaatgac aaactattga aagaagcaaa agataaatat
      961 tatcaattta ccaaaaaaga taaaagactt ttatccgaag cctccattca aaatgttact
     1021 atagaaacta tttctaccac agaaacaatt atttattaca ataactcatt tgataaagag
     1081 agaagaaaag tgaaagctgt aaaccaaaat aacacttggc tcatagattt taaatataca
     1141 tttaacccca atttataatg cttaaaaata ttttattagt tggcataggc ggtggtttag
     1201 gttctatttt tagatatttt attggtttaa tgattactaa caaacatttt ccttatgcta
     1261 ccattacagt aaatgttgta ggctcatttt ttattggtgt tattatggca attcttgcaa
     1321 aacaaacaca caatgtggag cattggtact taataattac tacaggcttt ttaggtggct
     1381 ttaccacatt ctctgctttt agctggaaca catttcagtt aatgcaacag caacaatatg
     1441 cagcatcttt tttatatatt gggcttacgt tttttttaac cttaatagct gttagtattg
     1501 gtttctttat ttttaaataa aaattatatt tttaaaatat gcaacaatta gttcaacatc
     1561 cttggcatgg tgttaaccca ggagaacaag caccaagaaa agtaaatgca gttattgaaa
     1621 ttccgcaagg tagcagatgt aaatatgaga ttgataaacc cagtggctta ttaaaattag
     1681 acagaataat ttattcttca ttttattatc cttgtaatta tggttttata cctcaaacct
     1741 atggtggaga caaagaccca ctagatatac ttgtaataac ttctattcct gttgttcctt
     1801 taaccctaat ggaagcaaaa gttgtaggag taatgcaaat gatagatagt ggcgatgctg
     1861 atgataaaat aattgcagta gcatctaacg acccaggtgt taaccattac aataatatag
     1921 aagaattacc caaacatttt tttgatgagt taaggcattt ttttgaagag tataaaaaat
     1981 tagaaaacaa agaggttaaa gtagaagaat ttggtgataa aactactgca ttaaaaattg
     2041 tttcagaagc aatagaattt tataaaaaat cttttttaaa taaataaaaa aactatgaca
     2101 gttaacacta ttattatatt aattattatt ggcgttgcag ctggcatgct tagtggttta
     2161 gtaggtgttg gtggtggaat aattattgta ccagcattaa tttatattct tggtttttct
     2221 caaaaaacag cacaaggtac ttcgttaggc ttattattat tacctgtatt tattgtgtcg
     2281 ttaatttatt actataaagc aggcgaggta aatataaaag caattccatt tttagccata
     2341 ggctttttag ttggcggata tttaggaagt aaattagcct tatcgctacc cgatgctact
     2401 gtaaaaaaaa tatttgcttt tttaatgatt attgtagcag taaaaatgct tttttttgat
     2461 aaacctaaaa aagatacaaa taaaatacac gccacagaag cacaaaaaaa taaaagcgtt
     2521 ggaagctaat tcctctaacc ctatctttgt aaaaatttga agaaaatgga aaaaacaaaa
     2581 tcaaaaagca agtactattt attatttatt ttttggttag ctatcatagt atttttat
//
LOCUS       DAOQPK010000277         2621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_31688_length_2621_cov_4.432970, whole genome shotgun sequence.
ACCESSION   DAOQPK010000277 DAOQPK010000000
VERSION     DAOQPK010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31688_length_2621_cov_4.432970"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..275
                     /gene="rpmD"
                     /locus_tag="PLU36_10140"
     CDS             93..275
                     /gene="rpmD"
                     /locus_tag="PLU36_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012789305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HMZ47153.1"
                     /translation="MAKIKITQIKSGIDRTELQKQTLIALGLRKLNASKEVEATPQIL
                     GMVNKVSHLIKVEEIA"
     gene            376..825
                     /gene="rplO"
                     /locus_tag="PLU36_10145"
     CDS             376..825
                     /gene="rplO"
                     /locus_tag="PLU36_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HMZ47154.1"
                     /translation="MKLHSLKPAKGSTHKEKRLGRGEASGKGGTSTKGNKGGQSRAGY
                     QRKNAHEGGQMPIQRRVPKRGFKNINRVEYQVFNLGQIEQLQEKYALPEFSLENLYVN
                     GLISRTDKVKVLATGEIKSKLNFKINAISDKAKAAIEALGGTVEIVK"
     gene            905..2260
                     /gene="secY"
                     /locus_tag="PLU36_10150"
     CDS             905..2260
                     /gene="secY"
                     /locus_tag="PLU36_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HMZ47155.1"
                     /translation="MKKLIQTIKNIWSIEELRGKIIVTLLLILLYRFGTHIVLPGLDA
                     AALESIKASNKTEGLLGLFDAFAGGAFSQASILALGIMPYISASIFMQLMAILVPQLQ
                     KIQKEGESGRKKINQWTRYLTVIVTAFQASAYIAYLNSTYSAAIIPAYASFFPFSTVI
                     ILTAGSLFVMWLGEKIQDKGLGNGASIIIMVGILARLPISIIQEWTAKGEKGGGGLLI
                     FLIEIAVFIAVVVGLVLLVQGVRKIPVNYAKQIVGNRQFGGARQFLPVKVNSAGVMPI
                     IFAQAIMFLPTLVSFTNLDSKEGIVRIFNDHTHVVYMLVYAVMVIGFTFLYTALIFNP
                     KQIAEDLKRNNGFIPGVKPGQPTADYIGSIMDKITLPGAIFLAFVGILPGFADRLGVT
                     QSFSTFFGGTSLLIMVQVILDTLQQIETYLLMRQYDGLMSSGRVMGRQSSNSSLPTTT
                     I"
     gene            2329..>2621
                     /locus_tag="PLU36_10155"
     CDS             2329..>2621
                     /locus_tag="PLU36_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HMZ47156.1"
                     /translation="MTLRPNGMIIYKTEAEVALMKQSALLVSKTLAEVAKVLKPGMNT
                     LEIDKMCEQFVRDHHAIPSFKNYKNTYPFSICASVNDVVVHGFPNTTPLKEGDV"
BASE COUNT          940 a          362 c          465 g          854 t
ORIGIN      
        1 ttaagtaaag tatttaacgg ataaattaaa ttgaaggcaa aaaattaaaa ctagttacgt
       61 taattttttt aaacttctaa aacttattta aaatggcaaa aattaaaata actcaaatta
      121 aaagcggtat agatagaact gaattacaaa agcaaacttt aatagcttta ggtcttagaa
      181 aattaaatgc atctaaagag gttgaagcta caccccaaat attaggaatg gtaaataaag
      241 taagccattt aattaaagtt gaagaaatag cttaaaatat taaatgtttg aactaataac
      301 tcaaacattt tattatttca taaattatca ttgcgagggg tgataccccg taagtccaaa
      361 atcaataaaa ataacatgaa attacattct ttaaaacctg caaaaggtag tacacacaaa
      421 gaaaaaagat taggccgtgg tgaggcaagc ggtaaaggag gaacatctac aaaaggaaat
      481 aaaggaggcc aaagccgtgc aggttaccaa cgtaaaaatg cacacgaagg tggtcaaatg
      541 cctattcaac gtcgtgtacc taaacgcgga tttaaaaata ttaacagagt agagtaccaa
      601 gtgtttaact taggtcaaat tgaacaatta caagaaaaat acgctttacc agagttttca
      661 ttagaaaatt tatatgttaa tggtttaata agcagaacag ataaagtaaa agtattagca
      721 accggtgaaa taaaatctaa actaaacttt aaaataaatg ccattagcga taaagcgaaa
      781 gcagcaattg aagctttagg cggtacagtt gaaattgtta agtaattttc agctatattg
      841 cacgacacat tttaaaatgt gtctttttga ttttatgtat taactgtttc aatcgaataa
      901 tcaagtgaaa aaactaattc aaactataaa aaatatatgg agcattgagg agctaagagg
      961 caaaatcatt gttacattat tattaatttt attataccgt tttggtacac atattgtttt
     1021 accagggtta gatgcagcag ccttagagag cattaaagca agtaataaaa ccgaaggttt
     1081 gttaggtttg ttcgatgcat ttgcaggtgg tgccttttca caagcttcta ttttagctct
     1141 tggcatcatg ccttacattt ctgcttctat atttatgcaa ttgatggcaa tcttagttcc
     1201 tcaattacaa aaaattcaaa aagaaggaga aagtggccgt aaaaaaataa accagtggac
     1261 tcgttacctt actgtaattg taactgcttt tcaggcaagt gcttatattg cttatttaaa
     1321 tagcacctat tcagcagcaa ttattcctgc ttatgcaagt ttctttccat tctctactgt
     1381 aattatactt acagcaggtt cattgtttgt aatgtggctt ggcgaaaaaa ttcaagataa
     1441 aggcttaggc aatggtgctt ctatcattat catggtgggt atcttagcta gattgcctat
     1501 aagcattatt caagagtgga ctgcaaaagg tgagaaaggc ggtggcggat tattaatatt
     1561 tttaattgaa atagctgtat ttattgcagt agttgtaggg cttgttttac ttgttcaagg
     1621 agttagaaaa attccggtaa actatgcaaa acaaattgtt ggtaatagac agtttggtgg
     1681 agcaagacaa tttttacctg ttaaagtaaa tagtgctggt gtaatgccta tcatatttgc
     1741 tcaggcaatt atgtttttac ctacattggt ttcatttaca aatttagatt ctaaagaagg
     1801 tattgttaga atatttaatg atcatacaca tgttgtttat atgttggtgt atgctgttat
     1861 ggtaattgga tttacctttt tatatactgc attaattttt aatcctaaac aaattgccga
     1921 agatttaaaa agaaacaatg gctttatacc tggtgtaaaa cctggtcagc caactgcaga
     1981 ttatattggc tctattatgg ataaaattac tttaccagga gctatatttt tagcatttgt
     2041 tggtatttta cctggctttg cagatagact aggtgtaacc caatcattca gtacattttt
     2101 tggaggtact agtttattaa ttatggtaca ggtaatttta gatactttac aacaaataga
     2161 aacttactta ttaatgcgtc aatacgatgg attaatgagt agtggtagag tgatggggcg
     2221 tcaaagttct aatagtagct taccaacaac aactatttaa tttttaagtt ataaatatac
     2281 tagaaacctg ttggagtaat atctaacagg tttcttactt tatatcaaat gacattaaga
     2341 cccaacggta tgataattta taaaacagaa gcagaggttg ctttaatgaa gcaatcagca
     2401 ttattggtta gcaaaacact tgcagaagta gctaaagttt taaaacccgg aatgaatact
     2461 ttagaaatag ataaaatgtg tgagcaattt gtaagagacc atcatgccat tccatctttt
     2521 aaaaattata aaaatactta tcccttttct atttgtgcaa gtgttaatga tgttgtagtt
     2581 cacgggtttc caaatactac tcctttaaaa gaaggggatg t
//
LOCUS       DAOQPK010000278         2615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_31803_length_2615_cov_5.082813, whole genome shotgun sequence.
ACCESSION   DAOQPK010000278 DAOQPK010000000
VERSION     DAOQPK010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31803_length_2615_cov_5.082813"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..197
                     /gene="ccoS"
                     /locus_tag="PLU36_10160"
     CDS             39..197
                     /gene="ccoS"
                     /locus_tag="PLU36_10160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HMZ47157.1"
                     /translation="MSVIIIMLIGSLLVAGGFLFAFLWCVKDGQYDDAYSPAHRILFD
                     DINTTTKK"
     gene            213..2333
                     /gene="ccoN"
                     /locus_tag="PLU36_10165"
     CDS             213..2333
                     /gene="ccoN"
                     /locus_tag="PLU36_10165"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HMZ47158.1"
                     /translation="MELEKFYYDNRIPKYFAIATMFWAVVGMLAGVLAAFQLAFPVLN
                     FDLAATTFGRLRPVHTNAVIFAFVGNGIFTAVYYSIPRLLKTKMYSDLLGKINFWGWQ
                     LIIVVGAITLMAGYTSGKEYAELEWPLDIAITIVWVIFGINMIGTILTRRERHLYVAI
                     WFFLATWVTVALLHIVNSFEVPVTLFKSYSMYAGVQDALVQWWYGHNAVAFVLTTPYL
                     GLMYYFVPKAANRPVYSYRLSIVHFWSLIFIYMWAGPHHLLYTALPQWAQSLGTAFSI
                     MLVLPSWGGMLNGLFTLRGAWDRVREDPILKFFVVGITCYGMATFEGPMLSLKNVNAI
                     SHYSDWTVAHVHIGALGWNGFLTFGMLYWLIPRLFKTELYSKKLASTHFWIGTLGIIL
                     YAIPMYWAAFRAYFMLKAFTPEGQLQYQFIDVVQTTVPFYAMRAVGGTVFLTGVLFMV
                     YNLYKTAKQGTFVEREEAEAAPLAKKYVSTGKSFWHSVIERKPILLTVLSLFVVGLGG
                     VFEIIPTFLVKSNIPTIASVKPYTPLELQGRDIYVREGCYNCHSQMIRPFRYEVMRYG
                     EYSKAGEFIYDHPFQWGSKRTGPDLARIGGKYPDSWHFNHMLEPTSMAPGSIMPKYPW
                     LFVRDLDISTTNSKIHAMRNLGVPYERGYEAKANDDLKEQSEKIANSLKADKIKISSK
                     KEIIALIAYLQRVGKDIKGEKTSN"
BASE COUNT          808 a          412 c          513 g          882 t
ORIGIN      
        1 gcattgcagt tttatatttt tgggcgtaaa tttaaaagat gagtgtaatc ataatcatgt
       61 taatcggtag cttgctcgtt gctggtggtt tcttgttcgc cttcttatgg tgcgtaaaag
      121 atggccagta tgacgatgct tactcaccag ctcatcgaat tttatttgac gatataaaca
      181 caactactaa aaaataatca atcagtatca atatggaatt agaaaaattt tattacgaca
      241 accgcatacc taagtatttt gctattgcga cgatgttttg ggcagtagtg ggtatgttgg
      301 caggtgtttt ggcagccttt caattagcgt ttccagtctt aaacttcgat ttagcagcaa
      361 ctacttttgg ccgtctaaga ccagtgcata caaatgcagt aatttttgct tttgttggaa
      421 atggtatttt cacagctgta tattattcta ttcctcgttt gttgaaaaca aaaatgtata
      481 gcgacttatt aggaaaaata aatttctggg gctggcaatt aattattgtt gttggtgcaa
      541 ttactcttat ggcaggctat acatctggta aggaatatgc tgaactagaa tggcctttag
      601 atatcgctat cactattgtt tgggtaattt ttggtattaa tatgattggc actatcttaa
      661 ctaggcgaga acgacattta tacgttgcca tctggttttt cttagctaca tgggtaacag
      721 ttgctttatt acatattgta aactcttttg aagttccagt aacattattc aaaagttatt
      781 ccatgtatgc tggtgtacaa gatgcattgg tacaatggtg gtatggtcat aatgctgtcg
      841 cattcgtatt aacaacaccc tatctaggtt tgatgtatta tttcgttcca aaagcagcga
      901 atcgtcctgt atattcatat agattaagta ttgtacactt ttggtcatta atatttattt
      961 atatgtgggc aggtcctcac catttattat atacggcatt gccacaatgg gctcaatcat
     1021 taggaacagc attttctatt atgttggttt taccaagttg gggcggtatg ttgaatggtt
     1081 tatttacact tcgtggtgct tgggatcgtg ttcgagaaga tccaatatta aaattcttcg
     1141 tcgtaggtat cacttgctat ggtatggcta cttttgaagg accaatgctt tctttgaaaa
     1201 atgtaaatgc aatttcacat tatagcgatt ggacagtagc gcacgtacat attggtgctt
     1261 taggctggaa tggtttctta actttcggta tgttgtattg gttaatacca agattgttta
     1321 aaactgaatt atactctaaa aaattagcaa gtacacattt ctggattggc actttaggta
     1381 tcatattata tgcaattcct atgtactggg ctgcattcag agcttacttt atgctaaaag
     1441 catttactcc agaaggtcaa ttgcaatacc aatttattga tgttgttcaa actactgttc
     1501 ctttctatgc aatgcgtgct gttggtggca cagtgttctt gactggtgtt ttgtttatgg
     1561 tttataactt atacaaaaca gctaaacaag gtacctttgt tgagcgtgag gaagcagaag
     1621 cagcaccatt agcaaaaaaa tatgtgtcaa ctggaaaatc attctggcac agtgttatcg
     1681 aacgtaaacc aatcttatta acggttttaa gtttattcgt tgtaggctta ggtggtgttt
     1741 ttgaaattat tccaacattc ttagttaaat caaatattcc aactattgca agtgtaaaac
     1801 cttacacacc attggaatta caaggtagag atatttatgt tcgcgaaggt tgttataact
     1861 gccactcaca gatgattcgt cctttccgtt acgaagtaat gcgatacgga gaatattcaa
     1921 aagcaggtga gtttatttat gaccatccat tccaatgggg aagcaaacgc acaggtccag
     1981 atttagcacg aatcggtgga aaatatccag atagctggca ttttaaccac atgttagagc
     2041 ctacttctat ggcgcctggt tctatcatgc ctaagtatcc gtggttgttt gttcgcgatt
     2101 tagatatcag caccacaaat tctaagattc atgctatgcg caacttaggt gtaccttatg
     2161 aaagaggtta tgaggcaaaa gcaaatgatg acttgaaaga acaatcagaa aaaattgcca
     2221 atagcttaaa agcagataaa ataaaaatta gcagtaagaa agaaattatt gcattgatag
     2281 cttatttgca aagagtaggt aaggatatta aaggcgaaaa aacaagcaac taatatcaaa
     2341 ttatcaccat taaaaacaga aaaatgaaat ttatcaatta tttagaaggc ataacaggag
     2401 tgagcgtatt tccgatgact tcattattgt tgttcgtcat attttttata ggcgtgatga
     2461 tttgggtaat gcgtgcagat acaggtttta tcaatcacat gagaaatata cctttggaaa
     2521 acgatataaa tgaaacgaat aaatagggaa aaataaaata gttgactaaa tcacggtcga
     2581 ctattttatt atagctgcta aaccaattac ttaaa
//
LOCUS       DAOQPK010000279         2613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_31847_length_2613_cov_4.768178, whole genome shotgun sequence.
ACCESSION   DAOQPK010000279 DAOQPK010000000
VERSION     DAOQPK010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31847_length_2613_cov_4.768178"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PLU36_10170"
     CDS             <1..298
                     /locus_tag="PLU36_10170"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HMZ47159.1"
                     /translation="REFNGKMYLMEHALYADFAIVKAWKGDKLGNLVFRKTTRNFSTS
                     MAKAGKITIAEVEELVEPGMLDPDSIHVAGVYVHRIFEGSNYEKRIERKTVRLK"
     gene            302..1048
                     /locus_tag="PLU36_10175"
     CDS             302..1048
                     /locus_tag="PLU36_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47160.1"
                     /translation="MFVSADFLAINYGVHDEIANFFTEREPPTNNLYWKDKLMYLRHE
                     PGYLFIPIIVDTLNRLGIKKQILLGNEYIHLLEEVGHIAALEETNSITKQKAIELCIN
                     LTQAKAKNKYFYDALEKYMTGDNNSFINELLLPYKALHRGDIFLFSVAILDFDNEVAH
                     KIIQYWFVIIGSFLLLDDASDIEADKQNNHENAFLECGLDAKGITEIKLFLGSLLTTI
                     KTFNKPLARAIDNQFVKMAELPHIKQYLKN"
     gene            1052..1687
                     /locus_tag="PLU36_10180"
     CDS             1052..1687
                     /locus_tag="PLU36_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HMZ47161.1"
                     /translation="MALDKFGIAKRIAQELKDGMYVNLGIGIPTLVANYIPDDITVIL
                     QSENGILGMGPYPTEDDLDADLINAGKETVTLIKGAALFDSAESFGMIRAGKIDLTVL
                     GAMEVSEKGDIANWKIPGKMVKGMGGAMDLVASAQNIIVAMMHTNPKGESKLLPSCTL
                     PLTGLGCVKKVVTDLAVLNITQDGFELIETAPGVTIDEVKAKTAGKLIVKM"
     gene            1701..>2613
                     /gene="pgi"
                     /locus_tag="PLU36_10185"
     CDS             1701..>2613
                     /gene="pgi"
                     /locus_tag="PLU36_10185"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615651.1"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HMZ47162.1"
                     /translation="MLSKINPTTTQAWLLLTKHFEAEMNHASMRKLFAENENRFQEFS
                     IIDGDILFDYSKNIINNKTIQLLLQLANECKLQEAINEMFSGAKINETENRAVLHTAL
                     RNFSDTPIYNDGEDVMPSIRKVLKQMETFCNKVHKKEWLGYTGKPIKYIVNIGIGGSD
                     LGPVMVTEALKPYWINSIETYFVSNVDGTHIAETLRKINAEETLFLIASKTFTTQETM
                     TNAHTARSWFIEFAKNEKYVAKHFIALSTNEQEVKKFGIDTANMFEFWDWVGGRYSLW
                     SAIGLSIALTIGYKNFEQLLQGAFDIDN"
BASE COUNT          978 a          331 c          471 g          833 t
ORIGIN      
        1 aagagaattt aatggcaaaa tgtatttaat ggaacatgca ctttatgccg attttgcaat
       61 agttaaagca tggaaaggtg ataaattagg taatttagtt tttagaaaaa caacaagaaa
      121 tttttcaacc tctatggcta aagcaggtaa aattaccatt gccgaagtag aagaattagt
      181 agagccaggt atgttagatc cagactcaat tcatgttgct ggtgtatatg tacacagaat
      241 ttttgaaggc agtaattacg aaaaaagaat tgaacgaaaa actgttcgtt taaaataaga
      301 tatgtttgta agtgcagatt ttttggcaat aaattatggt gttcatgatg aaattgctaa
      361 tttttttaca gaacgtgaac caccaaccaa taatctttat tggaaggata aattaatgta
      421 tttacgtcat gagccaggct atttatttat tccaattata gtagatacat taaataggct
      481 tggtataaaa aaacaaattt tattaggcaa tgaatatatt catttattag aagaagttgg
      541 gcatattgca gcattagaag aaaccaattc tattaccaaa caaaaagcta tagagttatg
      601 tattaatcta acgcaagcaa aagcaaaaaa taaatatttt tatgatgctt tagaaaagta
      661 tatgactggg gataataaca gttttataaa tgaattatta ttaccttaca aagccttgca
      721 ccgaggagat atatttcttt tttcggtagc cattttagat tttgataatg aagtagcaca
      781 taaaattata cagtattggt ttgttataat aggctctttt ttattacttg atgatgctag
      841 tgatattgaa gcagacaaac aaaacaatca tgaaaatgct tttttagaat gtggcttaga
      901 tgccaaaggc attacagaaa taaaattatt tttaggcagc ttactaacaa ccattaaaac
      961 ttttaataaa cctttagcta gagcaataga taatcagttt gtaaaaatgg ccgaactacc
     1021 acatataaaa caatatttaa aaaattaaat tatggcatta gataaatttg gaattgcaaa
     1081 aagaattgca caagaattaa aagatggtat gtatgttaat cttggaattg gtattccaac
     1141 tttagttgca aattatatac ccgatgatat tacagttatt ctacaaagcg aaaatggtat
     1201 tttaggcatg gggccttatc caacagaaga tgatttagat gcagatctta taaatgctgg
     1261 gaaagaaaca gttactttaa ttaaaggtgc tgctttattt gatagtgcag aaagttttgg
     1321 tatgatacga gccgggaaaa tagaccttac cgttttagga gcaatggaag taagtgaaaa
     1381 aggagacatt gctaactgga agataccagg caaaatggtt aaaggcatgg gtggtgcaat
     1441 ggatttagtt gctagtgccc aaaatataat tgttgcaatg atgcatacta accctaaagg
     1501 agaaagtaaa ttattgccat catgcacatt acccttaact ggtttaggtt gtgttaaaaa
     1561 agttgttaca gatttagctg ttttaaatat tacacaagat ggttttgaat taatagaaac
     1621 tgcaccagga gtaactattg atgaggttaa agctaaaact gcaggtaaac taattgtaaa
     1681 aatgtagatt aaacaatttt atgctttcta aaataaatcc aactactaca caagcctggt
     1741 tattgcttac aaaacatttt gaagcagaaa tgaaccatgc aagcatgagg aaactttttg
     1801 cagaaaatga aaatagattt caggaatttt caattattga tggggatatt ttatttgatt
     1861 attctaagaa tataattaat aacaaaacca ttcagctttt attacaatta gccaatgaat
     1921 gtaaactgca agaagctatt aacgaaatgt tttcaggtgc taaaattaac gaaaccgaaa
     1981 accgtgcagt tttacatact gcattaagaa atttttctga tacgccaatt tacaatgatg
     2041 gtgaagatgt tatgccatcc atcagaaagg ttttaaagca aatggaaact ttttgtaaca
     2101 aagtgcataa aaaggaatgg ttgggttata caggaaaacc tattaaatat attgtaaaca
     2161 ttggaattgg tggcagcgat ttaggccctg ttatggtaac tgaagcttta aaaccatact
     2221 ggattaattc tattgaaact tattttgtaa gcaatgttga tggtacacat attgcagaaa
     2281 ctttaagaaa aattaatgca gaagaaactc tatttttaat tgcatcaaaa acatttacta
     2341 ctcaggaaac catgactaat gctcatactg ctcgttcatg gtttattgaa tttgcaaaaa
     2401 acgaaaagta tgttgcaaaa cattttattg cacttagtac taatgaacaa gaggtgaaaa
     2461 aatttggtat tgatactgcc aatatgtttg aattttggga ttgggttgga ggaagatata
     2521 gcctttggag tgcaataggt ttatccattg cattaaccat tggttacaaa aattttgaac
     2581 agcttttaca aggtgcattt gatattgata acc
//
LOCUS       DAOQPK010000280         2604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_32048_length_2604_cov_4.760690, whole genome shotgun sequence.
ACCESSION   DAOQPK010000280 DAOQPK010000000
VERSION     DAOQPK010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32048_length_2604_cov_4.760690"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..853)
                     /gene="fabG"
                     /locus_tag="PLU36_10190"
     CDS             complement(98..853)
                     /gene="fabG"
                     /locus_tag="PLU36_10190"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HMZ47163.1"
                     /translation="MKLLENKTSIVTGAARGIGAAIAVKLAEHGSNIAFSYVSDSSAA
                     KAAELENQLTALGVKAKAYKSNAGDFAQCETFVNEVMADFGNIDVCVNNAGISKDNLL
                     LRMTAEQWDDVMNINLKSVFNITKLVTRPMMKAKAGSIINMSSIIGIRGNAGQSSYAA
                     SKAGIIGFTKSVALELGSRNIRCNAIAPGFIETDMTHYLKDGDAANDFLKKIPLGRFG
                     KADEIANTTLFLASDMSSYITGQVLSACGGLNI"
     gene            complement(1000..2511)
                     /locus_tag="PLU36_10195"
     CDS             complement(1000..2511)
                     /locus_tag="PLU36_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012788543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HMZ47164.1"
                     /translation="MKLLSPAISRLARLRYWRIEQWLQNPFAAQREVLQDLVTHAQYT
                     EIGRKYGLTNLFKIRDFKKAVPIHTYDDLKPYIERLMQGEQNLLWNTPVYWFAKSSGT
                     TSDKSKFIPVTEESLTNCHYQGTKDVLSTYYHLNPNSDLLTGKGLLIGGSHQILPVNN
                     EASYGDLSAVLFQNTPIWANWLRTPELSIALMDDWGEKVEAIAKNTIQENVTSISGVP
                     TWTLVLIKRILEITGKQTLKEVWPNLELYLHGGVSFTPYKEQFKHLIGNEIRYLNSYN
                     ASEGFFAAQNNFDDEGMLLFLDNGVFYEFLPIEEYGKENPETIGLRDVEIGKQYALVI
                     STNGGLWRYLIGDTIEFTSLNPFKINVSGRLKHFINAFGEEVIVDNTDKAIAQACKKC
                     NAKVNDYTAAPIYFTNNSNGAHEWLIEFEQLPTCLKEFTYELDCALKNINSDYEAKRH
                     LDIALRMPIVQAVDKSVFTNWLKSKNKLGGQHKVPRLSNDRKYIDEIKQFINN"
BASE COUNT          823 a          462 c          361 g          958 t
ORIGIN      
        1 ataagcaatg taccataact gtataaatca aaaataatga aaaatagttt tcagtataat
       61 tgaaaactat tttttaaatt gaaaggttag atggttttta aatgtttaat ccaccacatg
      121 cacttaacac ttgtcctgta atataagaac tcatatcgct agcaagaaat aaagttgtat
      181 tggcaatttc atctgcttta ccaaatctgc caagtggtat tttctttaaa aaatcatttg
      241 cagcatcacc atcttttaaa taatgtgtca tatctgtttc aataaagcct ggtgcaatgg
      301 cattacatct aatatttcta ctacccaact ctaaagctac actttttgta aagccaatta
      361 tacctgcttt acttgctgca tagctacttt ggcctgcatt tcctctaata ccaataattg
      421 aactcatatt aattatgcta ccagccttag ccttcatcat tgggcgtgta accaattttg
      481 taatattaaa aacgctttta agattgatgt tcattacatc atcccattgt tcagcagtca
      541 ttcttagtaa taaattatct ttgcttatgc ctgcattgtt tacacaaaca tcaatatttc
      601 caaaatctgc cattacctca ttaacaaagg tttcgcattg ggcaaagtct cctgcattgc
      661 ttttataggc tttagctttt actcctaatg ctgttaactg attttctaat tctgctgctt
      721 tggctgcact actatcacta acatagctaa aagcaatatt actaccatgc tctgcaagtt
      781 ttactgcaat ggctgcacct ataccacgag ctgctcctgt aacaatggag gttttatttt
      841 ctaacaattt cataagttta cttttattgg gtggttttta ttattcaatt aaaaaataat
      901 aattaataca aattaatgaa agtttttgaa tgaaaatgaa attgtagtaa aaaagaaaaa
      961 tgaactttga attaggagta atttttttag aaaagagtgc taattattaa tgaattgttt
     1021 tatttcatca atatattttc tatcgttact taaccttggc actttatgtt gcccacctaa
     1081 tttgttttta ctttttaacc agtttgtaaa aaccgattta tctacagcct gcacaatagg
     1141 cattcttaat gcaatatcta aatgtctttt agcttcataa tcgctattaa tattttttaa
     1201 tgcacaatct agttcataag taaattcttt taaacaagta ggtaactgtt caaactcaat
     1261 caaccattcg tgtgcaccat tgctattatt agtaaagtaa attggtgcag cagtataatc
     1321 attcactttt gcattacatt ttttacaggc ttgtgcaata gctttatcag tattatctac
     1381 aattacttct tcgccaaagg cattaataaa atgttttaat ctgccagata catttatttt
     1441 aaatgggttt aaagatgtaa actctatagt atcaccaatt aagtacctcc ataaaccacc
     1501 attagttgaa attaccaaag catattgctt gccaatttct acatctctta atccaatggt
     1561 ttcggggttt tctttaccat attcttcaat tggtaaaaat tcataaaaaa caccattatc
     1621 taaaaacaat aacattcctt cgtcatcaaa attattttga gcagcaaaaa agccttcgct
     1681 ggcattgtaa gaattgaggt aacgaatttc gtttcctatt aaatgtttaa actgttcttt
     1741 ataaggtgta aatgaaactc ccccatgcaa gtacaactct aaatttggcc aaacttcttt
     1801 aagtgtttgt ttacctgtaa tttctaaaat tcttttaata agtaccaatg tccatgttgg
     1861 aacacctgat atagaagtaa cattttcttg aatggtattt tttgctatag cttctacttt
     1921 ttcgccccaa tcatccatta aagcaatgga tagttctggt gttcttaacc agtttgccca
     1981 aatgggtgtg ttttgaaaaa gtactgcact taaatcgcca taagatgctt cattatttac
     2041 tggtaaaatt tgatggcttc ctccaattaa taatccttta cctgtaagta aatcgctatt
     2101 aggatttaga tgataataag tacttaatac atcttttgta ccttgataat ggcaattggt
     2161 aaggctttct tcagttacag gaataaactt acttttatcg ctggttgtac cactgctttt
     2221 tgcaaaccaa taaacaggtg tgttccaaag taaattttgt tcgccttgca ttagcctttc
     2281 tatatatggc ttaagatcat cgtatgtatg aattggaact gcttttttaa agtctcttat
     2341 tttaaataaa ttggttaacc catactttct gcctatctct gtatattggg catgggttac
     2401 taaatcttgc aatacttctc tttgtgctgc aaaggggttt tgcagccatt gctctattct
     2461 ccaataacgt aaacgagcaa gccttgatat ggcaggacta agcagtttca ttttaaaaat
     2521 aacgttagca agttaagcaa tgataagaaa aaaatacttg aaagtgatat tcttagagcg
     2581 tctctaatct ctttatcttg tacc
//
LOCUS       DAOQPK010000281         2590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_32358_length_2590_cov_3.187377, whole genome shotgun sequence.
ACCESSION   DAOQPK010000281 DAOQPK010000000
VERSION     DAOQPK010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32358_length_2590_cov_3.187377"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PLU36_10200"
     CDS             <1..363
                     /locus_tag="PLU36_10200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMZ47165.1"
                     /translation="GVFGKEKFKVIEASDIFIFPTYYPQEVFPGVVLEAMQCAKPIIT
                     TTTGVIPEIIDDGINGLIAHKKDTALFVELAESLINNKKMAMQMGKAAREKYLSSYTQ
                     EIFENNVKDTIINVVELN"
     gene            complement(355..933)
                     /locus_tag="PLU36_10205"
     CDS             complement(355..933)
                     /locus_tag="PLU36_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47166.1"
                     /translation="MFNIKQLIKNILSYPIAYFILLKTRKLIIGSSNKRIFLFDIDNT
                     IADSWHSLTLNIWANENERLKGLAIFLRMRKLVTILQKNKNNQVIFLTARSYFSRQTT
                     ENWLHENGILQTSKNLIITRTAKDKINIIKSLPLAKLKIFYIDDLSHKHEKGTVEMFE
                     DEINCIKKLEKLNTKNFTYYGKSEIDKFCSLV"
     gene            1042..2337
                     /locus_tag="PLU36_10210"
     CDS             1042..2337
                     /locus_tag="PLU36_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47167.1"
                     /translation="MELAGINSRNIANGLYIINQYPKYLQSQYFSAEKIKLIQLKKLE
                     AIVRVAINTVPYYKALNVKINFNNFSQQELLKFPIITKAIIRDNYNSFISEKYFNKKL
                     QYKSTSGSSGQPFKVPKPFYSDGIEQCMMFRAWSMNPAFKYSFHKPCIVLRSFSPKQG
                     EPPYKVDKILNYWYLSPFDINQQNLETYVQFIKKSKAQLLRGYPSSIYILTLLLKENN
                     IKLPQIKTLFTSSENLLPNYRKVIEDYWQMPLIDWYGQNERTVTVQMCEHGNYHNNDE
                     YGIVELDENNQIIATSLNNNIMPLIRYATNDIAIPAINPSCNCGRGLSIPFSGIDGRA
                     DDLLIKEDNTIIATVNIYNAMEKFPDVKQFKIIQQKDKSLELYLSENTKLSPEYICNI
                     EKELKQRLGNVRIITKVVEEIERNKNTEKIKSIESHIKL"
     gene            2341..>2590
                     /locus_tag="PLU36_10215"
     CDS             2341..>2590
                     /locus_tag="PLU36_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47168.1"
                     /translation="MPIKGLTNEQAAMVQHIKLLKQQAGSHSPSLFTIKEQLPDLKMN
                     IDACFLSNPYATDLFLRYFKNELLGDDLLIRDMLEFYPS"
BASE COUNT          974 a          347 c          370 g          899 t
ORIGIN      
        1 ggggtgtttg gtaaagaaaa atttaaagta atagaagcat ctgatatttt tatttttcca
       61 acttattacc ctcaagaggt ttttcctgga gttgttttag aagctatgca atgtgctaag
      121 ccaattataa caactacaac aggtgtaata cctgaaatta ttgatgatgg aataaacggt
      181 ttaattgcac ataaaaagga tactgcttta tttgtagaat tggcagaaag tttaatcaat
      241 aataaaaaaa tggctatgca aatgggtaag gctgcaagag aaaaatattt aagcagctac
      301 actcaagaaa tttttgaaaa caacgtaaag gatacaatta ttaatgttgt agagttaaac
      361 taaactacaa aacttgtcaa tttcactttt gccatagtaa gtaaaatttt tagtatttaa
      421 tttctctaat tttttaatac agttaatttc atcttcaaac atttccactg ttcctttttc
      481 atgtttgtga gataagtcat ctatataaaa aattttcaat tttgctaatg gtaaactttt
      541 tataatatta attttatctt ttgcagttct ggtaataatt aaatttttag aggtttgtaa
      601 aatgccattt tcatgtaacc agttttcggt tgtttgcctc gaaaaataac tacgtgcagt
      661 taaaaaaata acttgattgt ttttattttt ttgcaatatt gttactagtt ttctcattct
      721 taaaaaaatg gccaaccctt ttaacctttc gttttcgttt gcccaaatgt taagtgttaa
      781 agaatgccaa ctatctgcaa tggtattatc aatatcaaat aaaaaaattc ttttattgct
      841 actaccaata attaattttc ttgtttttaa taatataaaa taagcaatag ggtagcttaa
      901 aatatttttt attagctgct ttatgttaaa catattttgt gtaagtaatt accaatacaa
      961 atatgcagca aaataattgt tcaatttatt tttctttctt tttataaaat cacacaataa
     1021 atttttaggt aatttaagtt tatggagcta gcaggaataa atagcagaaa tattgccaat
     1081 ggtttataca taattaacca atatccaaaa tatcttcaat cgcaatattt ttctgccgaa
     1141 aaaattaaat taatacagct aaaaaagtta gaagctattg taagggtagc aataaatact
     1201 gttccttatt ataaagcact aaatgtaaaa ataaatttta acaacttttc tcagcaagag
     1261 ttattaaaat ttccaataat taccaaagct ataataagag ataattacaa cagttttatt
     1321 tcagagaaat atttcaataa aaagctgcaa tataaatcta cttcaggctc cagtggacag
     1381 ccatttaaag tgccaaaacc tttttatagc gatggtattg agcaatgcat gatgtttagg
     1441 gcttggtcta tgaatcctgc atttaagtat agctttcata aaccttgtat tgtgttaagg
     1501 agcttttcgc caaaacaagg tgagccgcct tataaggtag ataaaatttt aaattactgg
     1561 tacttatctc catttgatat aaatcaacaa aacttagaaa cctatgtaca gtttattaaa
     1621 aaatctaaag cacaactgct acgtggttat ccaagctcta tttatattct tactttatta
     1681 ttaaaagaaa ataatattaa gttacctcaa attaaaaccc tttttacatc gtccgagaat
     1741 ttattgccta attatagaaa agtaattgaa gattattggc aaatgccttt aatagattgg
     1801 tatggacaaa atgaaagaac tgttactgtg caaatgtgtg aacatggaaa ttatcataac
     1861 aacgatgaat atggaatagt agaattagat gaaaataatc aaattattgc tacttcttta
     1921 aacaacaata ttatgccttt aataaggtat gcaactaacg atattgccat acctgctatt
     1981 aatccttctt gcaattgtgg acgaggactt agcattcctt ttagtggtat tgatggcaga
     2041 gcagatgatt tattaattaa agaagataat accattattg ctactgtaaa tatttacaat
     2101 gcaatggaaa aatttccaga tgtaaaacag tttaaaataa ttcaacaaaa agataaatct
     2161 ttagaattat atctttcaga aaacaccaaa ctttctccag agtatatttg taatattgaa
     2221 aaggagttaa agcaacgatt aggtaatgta cgcattataa ctaaagtggt agaggaaata
     2281 gaaagaaata aaaataccga aaaaattaaa tcaattgaat cacatataaa attataaagt
     2341 atgccaatta aaggattaac taacgaacaa gctgcaatgg tgcagcatat aaaattgcta
     2401 aaacaacaag ctggctcaca ctcgcctagt ttgtttacta taaaagagca attaccagat
     2461 ttgaaaatga atatagatgc atgttttctt tcaaacccat acgcaacaga tttgttttta
     2521 agatatttta aaaatgaatt gttgggagat gatttattga taagagatat gttggagttt
     2581 tatccttctc
//
LOCUS       DAOQPK010000282         2586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_32438_length_2586_cov_3.958910, whole genome shotgun sequence.
ACCESSION   DAOQPK010000282 DAOQPK010000000
VERSION     DAOQPK010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32438_length_2586_cov_3.958910"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..146
                     /locus_tag="PLU36_10220"
     tRNA            76..146
                     /locus_tag="PLU36_10220"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:108..110,aa:Cys,seq:gca)
     gene            255..932
                     /locus_tag="PLU36_10225"
     CDS             255..932
                     /locus_tag="PLU36_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47169.1"
                     /translation="MKKNVLFITLFLFFGVANAQKLVKLIQKNDAYFVEYDNKVQKEI
                     IQGKGNSVIYYSKKNQYVLYKKLIKKSVLMNKEDGREDAADMYSIYIFNILNNKTIEV
                     FKSCLDGNDGTKPSYANSKIYPFNSICNPDNFMLSPDENIIFFNSKAWAVSNAIHYFS
                     INDLMLKFFHSGSLIKVDNNGVTIEISDVETVRENGEIVSKGRYWQKCLYDFNGKFIK
                     YIGEKNR"
     gene            978..>2586
                     /locus_tag="PLU36_10230"
     CDS             978..>2586
                     /locus_tag="PLU36_10230"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HMZ47170.1"
                     /translation="MQKSSTLLLQFFYLILLVVCSYTLCRIFFVLFNLKELDIPTFNS
                     FATILWHGLRFDVAAIAVTNSLFIILFFLPVKLATNKLYQLVLKVIFIIINSFCIIAN
                     LMDVAYFPFVHKRSQADALLFFNGQKGNDFFRLIPTFLAQYWYLLFIVGLLIYLLYFF
                     YKKIIINSNLKNTLTIKHYSLRAIVFLMAIGITIIAIRGGFQTKPLDIIHASEVVSAK
                     NIAAVINTPFSIIKTVDKKSLEEKIYFPTSVLSELNNGIHLPPSNHSFTKKNVVIIIL
                     ESVSKRHIHYFGSKAATPFLDSLFNQSLVFTNAFANAKESIQGIPAILSSIPSLQTDP
                     FIFSSYASNKITSLPEVLKAEGYTTSFFHGGFNGTMGFNSYAKLAAFDNYYGMNEYNN
                     SKDYDGKWGIWDEPFLQFTSQKISETKEPFMASIFTLNTHHPFQIPQKYSHKFNKHQE
                     PFLNCIEYLDFALQQFFNISKNKPWFNNTLFVITADHTAPLLKNETTTLMDDFRIPLV
                     FFQQNNHDLKGINNSIANQIDILPSVLA"
BASE COUNT          905 a          381 c          352 g          948 t
ORIGIN      
        1 cgcttccagc aactggaaaa aaaatgaagc ctcacttgct aattatcaat ttctttatac
       61 atttgtcgcc ctaatggtcg tgtggccgag tggctaggca gagctctgca aaagcttcca
      121 caccagttcg aatctggtcg ggacctcttt cctaactttt tttcttatta tttaccttaa
      181 ctttatttgc tctaaccccc tttcttctta actacaattg gtatatttga gaattaaagt
      241 ataaattaat gtgtatgaaa aaaaatgtat tgtttattac attattttta ttttttgggg
      301 tagcaaatgc acaaaagcta gttaaattaa ttcaaaaaaa tgatgcctat tttgttgagt
      361 atgataataa agtacaaaaa gaaataatac aaggaaaagg aaattctgtt atatattact
      421 ctaaaaaaaa tcagtacgtt ttatacaaaa aattaattaa aaaatctgta ttaatgaata
      481 aagaagatgg aagagaagat gctgcagata tgtattctat ctatatattc aatatattaa
      541 acaataaaac cattgaagta tttaaatctt gtttggatgg aaatgatggt acaaaaccaa
      601 gttatgcaaa ttctaaaatc tatccattta actcaatttg taatccggat aattttatgt
      661 tgagcccaga tgaaaatatt attttcttta actccaaagc atgggctgta tctaatgcta
      721 ttcactattt tagtattaac gatttaatgt taaagttctt tcattctggt tcactaatta
      781 aggttgacaa caatggtgta acaattgaaa tatctgatgt tgaaacagtt agagaaaatg
      841 gtgaaatagt tagcaaagga cgttattggc aaaaatgttt atacgatttt aacggtaaat
      901 ttattaaata tattggcgaa aaaaacaggt aaaaaagtag tgcttaacta taattttgct
      961 actgaattta ttagagaatg caaaaatctt caaccctact tcttcagttt ttctatctta
     1021 ttttacttgt cgtttgtagc tatacgcttt gtagaatatt ttttgtttta tttaatttaa
     1081 aagaattaga cattccaacc tttaattcat ttgcaactat actatggcat ggtttaagat
     1141 ttgatgttgc agccattgca gttactaata gtttatttat aattttattt tttttaccag
     1201 taaaattagc aaccaataaa ctatatcaat tagtgttaaa agttattttt attataataa
     1261 atagcttttg cattatagcc aatttaatgg atgttgcata ttttccgttt gtacacaaac
     1321 gttctcaggc agatgcatta ttatttttta atggtcaaaa aggtaatgat ttttttaggc
     1381 ttatccctac ttttttagct cagtattggt atttactatt tatagttgga ttactaattt
     1441 atttgttata ttttttttac aagaaaatta tcatcaatag taacctaaaa aatactttaa
     1501 ctattaaaca ttacagttta agagcaattg tatttttaat ggctattgga attacaataa
     1561 ttgcaattag aggaggtttt caaacaaaac ctttagatat tattcatgcc tcagaagtag
     1621 tttctgcaaa aaatattgct gcagtaataa atacaccatt ttccatcatc aaaacagtag
     1681 ataaaaaaag tttagaggaa aaaatatatt tccccacctc tgtattatca gaattgaata
     1741 atggcattca tcttccacct tccaatcatt cttttacaaa aaaaaatgta gttattatta
     1801 ttttagaaag tgtttctaaa agacacattc attattttgg aagtaaagct gctactccat
     1861 ttttagactc cttatttaac caaagccttg tttttactaa tgcctttgcc aatgcaaaag
     1921 aatccattca aggcattcct gcaatacttt cttctattcc ttctttgcaa acagatcctt
     1981 ttattttttc ttcttatgca tccaacaaaa ttacatctct accagaagtg ttgaaagcag
     2041 aaggatatac aaccagcttt tttcatggag gttttaatgg tacaatgggc tttaacagct
     2101 atgctaaact tgctgctttt gataattatt atggtatgaa tgaatataat aattcaaaag
     2161 attatgatgg aaaatgggga atatgggacg aacctttttt gcagtttact tcccaaaaaa
     2221 tatctgaaac taaagagcct tttatggctt ccatttttac tttaaataca caccatcctt
     2281 ttcaaatacc tcaaaagtat tcacataaat ttaataagca ccaggagccg tttttaaatt
     2341 gtattgaata tttagatttt gcattacaac agttttttaa tatttcaaaa aacaaacctt
     2401 ggtttaataa tactttgttt gttataacag ccgaccatac tgcaccattg ctaaaaaatg
     2461 aaacaacaac actgatggat gattttagaa ttccattggt attttttcag caaaataacc
     2521 atgatttaaa aggcataaat aatagcattg ccaaccaaat agatatttta cccagcgttt
     2581 tagcta
//
LOCUS       DAOQPK010000283         2580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_32595_length_2580_cov_3.971485, whole genome shotgun sequence.
ACCESSION   DAOQPK010000283 DAOQPK010000000
VERSION     DAOQPK010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32595_length_2580_cov_3.971485"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2232)
                     /locus_tag="PLU36_10235"
     CDS             complement(<1..2232)
                     /locus_tag="PLU36_10235"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HMZ47171.1"
                     /translation="MKDFSYITNSHPSFIENLYNEFLKDPNSIDPDLKKFFEGFDFAV
                     NFGNFNQIISSDQTSSATTSSSQNIDWMNEIKVYRLILGYRNKGHLLANTNPIRPRKD
                     RGANLDLNFFGLSDADLNQTFYAGNLIGLGPTTLKNILEYLQKIYANDVGIEFKYISN
                     QNKIDWLVNEIEQKFSSPLSLNKKKRILEKLNEGVMFEKFLHTKYIGQKRFSLEGGEN
                     TITALDQIITTAANNNVEEVVIGMAHRGRLNVLANIMGKTYEQIFSEFEGTGSIDQTM
                     GSGDVKYHMGFGSKVTTEDGKEVHLKLMPNPSHLEAVNPVVEGFSRAKADILYNSEYK
                     KILPILIHGDSSVAGQGIVYEVLQMSELEGYDTGGTIHFVINNQIGFTTDFDDARSAD
                     YCTSIAAMVQAPVLHVNGDSAEAVIKCAEIATRYRQQFNSDIFIDMVCYRKHGHNEGD
                     DPKYTQPKLYALIDKHANPREVYVKHLLDNGESDAQNLAKEMEKKFWADLQERLDEVK
                     QKPLPYNYQQSELAWKSLRRAKAEDFDFSPSTAISDTEIRRLFNGLMHIPSNFQPLKK
                     IEKLLFDKVKLLETENKIDWATAELLAYSSILIEGKIVRMSGQDVKRGTFSHRHAVIR
                     DEVTDEFYNRLNSIQPNQEKFRIYNSLLSEYGVLGFEYGYAMANPNALTIWEAQFGDF
                     CNGAQTMIDQFIASSEQKWNRMNGVVMLLPHGYEGQGPEHSSARLERFLQLSAELNMV
                     ITNI"
BASE COUNT          781 a          455 c          388 g          956 t
ORIGIN      
        1 aatattggta ataaccatgt tcagctctgc agagagttgt aaaaaccttt ctagtcttgc
       61 actgctatgc tcaggccctt gcccttcgta gccatgaggc aaaagcataa ctacaccatt
      121 cattctattc catttttgtt cactagatgc aatgaattga tctatcattg tttgagcacc
      181 attacaaaag tctccaaact gggcttccca aatagttaaa gcatttgggt tggccattgc
      241 atatccatat tcaaacccta aaacaccata ttcacttaat aaagaattat aaattctaaa
      301 tttttcttga tttggttgaa tggagtttaa acgattataa aactcatccg ttacctcatc
      361 tctaattact gcatgacgat gagagaaagt gcctctttta acatcttgcc cactcattct
      421 cacaattttt ccttcaatta aaatagagga ataagctagt aattcggctg ttgcccaatc
      481 tattttattt tcagtttcta atagttttac tttatcgaat aataatttct ctattttttt
      541 taagggctga aagttagatg gaatatgcat taaaccatta aataaccttc taatttcggt
      601 atcactaatt gctgtagaag gagagaagtc aaagtcttct gctttggctc ttcttaaact
      661 cttccatgca agttcagatt gttgatagtt gtatggtaaa ggcttttgct ttacttcgtc
      721 taatctttct tgtaagtctg cccaaaactt tttctccatt tctttcgcta aattctgtgc
      781 atcagattcg ccattgtcta ataaatgttt aacatacact tctcttggat ttgcatgttt
      841 atcaattaat gcataaagtt ttggttgggt gtatttaggg tcatcgcctt cattatggcc
      901 atgtttacga taacaaacca tatctataaa aatatcgctg ttaaattgtt gacgataacg
      961 agttgcaatt tctgcacatt ttattacagc ttcggcacta tcgccattta catgcaaaac
     1021 aggggcttgt accattgcag caatagatgt acaatagtct gcacttctgg catcatcaaa
     1081 atctgtagta aaccctattt ggttattaat aacaaagtgt atagtgccac ctgtatcgta
     1141 accttctaat tcactcattt gtaatacctc atatacaatt ccttgcccag ctaccgatga
     1201 gtcgccatga attaatatgg gtaatatttt tttatattca ctattgtaaa gtatatcggc
     1261 ttttgctctt gaaaatcctt ctacaacagg gtttacagct tctaaatgcg atgggtttgg
     1321 cattagtttt aaatgaactt cttttccatc ttctgtggta actttactgc caaatcccat
     1381 gtggtatttt acatctccac tacccatggt ttgatctatt gatcctgtac cttcaaactc
     1441 actaaaaatt tgttcgtagg ttttacccat aatattagca agtacattta atctgcctct
     1501 gtgtgccatg ccaattacca cttcctcaac attattattg gctgcagttg taataatttg
     1561 gtctaatgct gtaatagtat tttcgccacc ttctaatgaa aatctttttt gacctatgta
     1621 ttttgtatga agaaattttt cgaacataac cccttcattc aatttctcta aaattctttt
     1681 ctttttattt aaagatagtg gagagctgaa tttttgttct atttcattca ctaaccaatc
     1741 aattttattt tgattgctga tatatttaaa ttcaatacca acatcattgg cataaatttt
     1801 ttgtaagtat tctaaaatat tttttaatgt ggtggggcct aacccaatta aattacctgc
     1861 ataaaaagtt tggtttaaat ctgcatcgga taaaccaaaa aagtttagat ctaaatttgc
     1921 acctctgtct tttcttggtc taatggggtt ggtatttgct aataagtggc ctttatttct
     1981 ataacctaat ataagcctat aaacctttat ttcattcatc caatcaatgt tttgagaaga
     2041 tgaagtggta gcagaagatg tttgatcaga actaataatt tgattaaaat ttccaaaatt
     2101 tacagcaaag tcaaaacctt caaaaaattt tttcaagtca gggtcaatgc tattagggtc
     2161 ttttaaaaat tcattgtata aattttcaat aaaacttggg tgggaattgg taatgtaact
     2221 aaaatccttc attattggtt gaaattaaac ttaattatag atagatttgt tggcaaatat
     2281 cgggctttga ggccataaaa aaactaaagt tttgtactaa taattcttta atcaatatta
     2341 agggattttc taagaattag ctctttgatg ttaaattgat tatttatata tttatatgtt
     2401 caaataggag gtcattccta ctatgagtgt aatactgagc atttctttaa gtcatcctga
     2461 gtggagcaaa ggatcttctg aataacatta aaggaacaag cagatgtttc acttcgttca
     2521 acatgacggt actatcttat aaactgtgta agtcttcata aagttggggt accccaactt
//
LOCUS       DAOQPK010000284         2557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_33125_length_2557_cov_5.031575, whole genome shotgun sequence.
ACCESSION   DAOQPK010000284 DAOQPK010000000
VERSION     DAOQPK010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33125_length_2557_cov_5.031575"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1427)
                     /locus_tag="PLU36_10240"
     CDS             complement(<1..1427)
                     /locus_tag="PLU36_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HMZ47172.1"
                     /translation="MQHIHKYDAQGKQLCCTQEEKINLIANKQLKKEHKEVGCCEADE
                     PKHSEHSDDDGHDHSSGSDSTFKMFLPAIISLVLLLIAIAFDNWFPQSWFRGWVRIIW
                     YIVAYAPVGLPVLKEAIQSIGKGEVFSEFLLMSIATIGAFAIGEYPEAVAVMLFYAVG
                     EVFQSLAVKRAKANIKTLLDQRPDEVTIIENNQPKTIKAETVQIGNFIQLKPGEKLGL
                     DGELLTETASFNTSALTGESKPDTKAKGETVLAGMINLNTVAQVKVTTAYTDSKLSKI
                     LELVQNATAQKAPTELFIRKFAKIYTPIVVLLALLITALPYFFVENYVFSQWLYRALV
                     FLVISCPCALVISIPLGYFGGIGAASKNGILFKGSNFLDIIANIQNVVMDKTGTMTEG
                     VFKVQEVILKPEFNKNEILQMVNALESQSTHPVATAIHNYVGKVDSNIKLENVEEISG
                     HGLKADVNGKELLVGNFKLMDKFNI"
     gene            complement(1432..2031)
                     /locus_tag="PLU36_10245"
     CDS             complement(1432..2031)
                     /locus_tag="PLU36_10245"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HMZ47173.1"
                     /translation="MNWLKIYLPIYLLLYLSVAFLIPTYRTYKKTGINPITFGKADNA
                     HDYIGLIMKILICLLFGTVLLFSFSQSLYYYLSPILYLEDPLILIIGIVIIHISLVWI
                     CIAQYQMSDSWRIGIDEINKTELITKGIFSISRNPIFLGMIVSVLGLFFVIPNALMFC
                     LTITTYIVIQIQIRLEEQFLQKQHGQVYINYKQKTRRLI"
     gene            complement(2043..>2557)
                     /locus_tag="PLU36_10250"
     CDS             complement(2043..>2557)
                     /locus_tag="PLU36_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47174.1"
                     /translation="KVGQTIHFTLTNNPTTEYDAVVYSIGTAFENDSKTIPVHCNVKG
                     NKSGLIDGMNITGIVSLNNVTTPAVPTDAIVEADGKFYIFIVTNKEPEEHHEEGEEGQ
                     DHSEAEKGKETEPKKHTNFEKIEVAKGVSNMGYTSITFINEIPKDAKIVSKGAFFINA
                     KLSNTGGHEH"
BASE COUNT          769 a          433 c          390 g          965 t
ORIGIN      
        1 ataatattga atttatccat tagcttaaaa ttgcctacta ataattcttt tccgtttaca
       61 tcagctttta atccgtgacc tgatatttct tctacatttt ctaactttat attgctatca
      121 accttaccta catagttatg aatagcagta gcaactgggt gtgtactttg actttctaaa
      181 gcatttacca tttgcagtat ttcattttta ttaaactctg gttttagtat tacttcttgc
      241 actttaaaga caccttcggt cattgtgcct gttttgtcca ttactacatt ttgaatattt
      301 gcaataatgt ctaaaaaatt actgccttta aacagaatac catttttaga agctgcacct
      361 atgccaccaa aataacctaa tggaatacta attactaatg cacaagggca actgataact
      421 aagaatacta atgctctata taaccattga ctgaacacat aattttctac aaagaagtaa
      481 ggaagtgctg taattagtaa ggcaagtagt acaacaatgg gtgtatatat ttttgcaaat
      541 tttcttataa acaattcggt tggggctttt tgtgctgtgg cgttttgtac taattctaaa
      601 attttactca acttactatc agtgtatgct gttgtaactt taacttgtgc tactgtgtta
      661 aggtttatca ttcctgctaa taccgtttcg cctttggctt tggtgtctgg tttgctttcg
      721 cctgtaagtg ctgaagtgtt gaaagaagca gtttcagtta aaagttctcc atctaaacct
      781 aatttttctc ctggtttaag ttgtatgaaa ttacctattt gaacagtttc agcttttatg
      841 gttttaggtt ggttattttc tataattgtt acttcatctg gtcgttggtc aagcaaggtt
      901 tttatattag ctttagctct ttttacagct aaggattgaa agacttcgcc cacagcatag
      961 aataacatta ctgctacagc ttctgggtat tcgcctatgg caaatgctcc aatagtggca
     1021 atactcatta ataaaaattc tgaaaatact tctcctttgc ctatgctttg aattgcttct
     1081 ttcaatacag gtaagccaac tggtgcataa gctactatat accaaataat acgaacccaa
     1141 cctctaaacc aagattgagg aaaccaatta tcaaaagcaa tagcaatgag taacaaaacc
     1201 aaacttatta tggctggtaa aaacattttg aaagtactat cgctaccact tgaatggtcg
     1261 tgtccgtcat cgtctgaatg ttcgctatgc ttgggttcat ctgcttcaca gcaacctact
     1321 tctttatgtt ctttttttaa ttgcttattg gcaataagat ttattttttc ttcttgtgtg
     1381 caacaaagtt gtttgccttg tgcatcgtat ttatgaatgt gttgcataat attatattaa
     1441 tcttcttgtt ttttgtttgt aattgatata aacttgaccg tgttgctttt gtaaaaactg
     1501 ttcttctaac ctaatttgaa tttgaatgac tatgtaagtg gtaatcgtta agcaaaacat
     1561 taacgcattt ggaattacaa aaaataagcc caatacactt acaatcattc ctaaaaaaat
     1621 aggatttcta cttattgaaa aaatgccttt tgtaattaat tcagttttat tgatttcatc
     1681 tataccaata cgccaactat cactcatttg atattgagct atacatatcc aaactaaaga
     1741 aatgtgaatt attacaatac ctataattag aattaatgga tcttccaaat aaagaatggg
     1801 acttaggtaa taataaaggc tttgtgaaaa tgaaaacaat aagactgtac caaataaaag
     1861 acaaatgagt attttcatta tcaatccaat ataatcgtgt gcattatcag ctttgccaaa
     1921 tgtaattgga tttatacctg ttttcttata ggtacgataa gttggaatta gaaatgctac
     1981 tgataaataa agtagtaagt aaataggtaa ataaattttt aaccaattca taattttata
     2041 ttttaatgtt cgtgtcctcc tgtgtttgat aattttgcat tgataaagaa agcacccttt
     2101 gatactattt tagcatcctt tggtatttca ttaatgaatg taatagaagt ataacccata
     2161 ttggaaacac cttttgccac ctctattttt tcaaaattag tgtgtttttt aggttctgtt
     2221 tccttgcctt tttctgcttc gctatggtct tgtccttctt ctccttcttc gtggtgttct
     2281 tctggctctt tgttggttac gataaaaata taaaatttgc catcggcttc tacaatagca
     2341 tctgtaggca ctgctggtgt tgttacatta tttaagctaa caatacctgt tatgttcatt
     2401 ccatcaatca accctgattt attgcctttt acattgcaat gtacaggtat ggttttgctg
     2461 tcgttttcaa aggcagtacc tatactatat acaactgcat cgtattcagt agttgggtta
     2521 ttagttaatg taaaatgaat ggtttgccca actttta
//
LOCUS       DAOQPK010000285         2550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_33295_length_2550_cov_3.864529, whole genome shotgun sequence.
ACCESSION   DAOQPK010000285 DAOQPK010000000
VERSION     DAOQPK010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33295_length_2550_cov_3.864529"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2212)
                     /locus_tag="PLU36_10255"
     CDS             complement(<1..2212)
                     /locus_tag="PLU36_10255"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ47175.1"
                     /translation="MKLLIILAIVGIALTGNAQFTKINTDSDIAYKMAKEYWQKEQYS
                     LAYPIFKNIYTQDNYNSNLKEHIKTESKFYFIICGLQLNDPTIETLAVDFITLEANAQ
                     KVGIVSFYLGEYYYRKKDFNTANNYFEKASIENLTNQQIAQMKFHQAYGYFTTQNFVK
                     AKPLFNSIRQLTESPNYIDANYYYGFIMFFEKKYKEALESFKLVEKTETYQNIVPFYI
                     AEIYYFAGERDKAIEYAKTALQKGNQYYKLELTQLIGHSLFEKRMYKEALPYLEEYVT
                     KSKKVRREDLYELAYCYYEAGKWNKAIDGFKQLGGKEDSLAQNSMYLLADAYLKIDDK
                     VGARNAFLFCATNSSNAVQKEVSQFNYAKLSYEQGYLDIAHKEIKSFLKTYPKSVYLN
                     EAKELQIAILANTSNYREAMALIDSLPQQSENIQKIYPKILYGRAVENINDQQLVVAN
                     ELLSKLLQVPFNANYIQYTYFWKGEIAYRNGNTNEALDYFTLYLKNPQTNEEINTNNA
                     RYNAAYCFLKKENYTAALELFQKVVLNVSASSSLLIQDAYIRSADCFFMLKNYNQALK
                     MYEVVIEQNLSQADYALYQKAIIAGANNKFTEKINLLQSIAIRYPNSPLITEAYLEVA
                     NTYLAEEKWADAIPYLDKVIKDKNATAFHPQAYLNNGIAYFNLTKYANALELFKTLVI
                     KYPNSPESDGAIEYIRKIFIEDQKPGEFISFMRENGKPVSYNEEDSLTFKAALIK"
BASE COUNT          797 a          357 c          371 g         1025 t
ORIGIN      
        1 actttattaa tgctgcttta aaggttaagg agtcttcttc gttataagaa actggtttac
       61 cattttctct cataaaggat ataaactctc ctggtttttg gtcttcaata aatatttttc
      121 taatatattc tatggctcca tcactttctg gagaatttgg atattttata accaatgttt
      181 tgaacagctc taatgcattg gcatattttg ttaagttaaa atatgcaata ccattattta
      241 agtatgcttg aggatgaaag gcagttgcat ttttatcttt aattacttta tctaaatatg
      301 gaatagcatc tgcccatttt tcttctgcca aataagtgtt agctacttct aaataagctt
      361 ctgttattaa tggagaattt ggatatctta ttgctatact ttgtagtaaa tttatttttt
      421 ctgtaaactt attattggca cctgcaataa ttgctttttg gtataaggca taatctgcct
      481 ggcttaaatt ttgctcaatt actacttcat acatttttaa agcctggttg taatttttta
      541 acataaaaaa acaatcagca cttcttatat aagcatcttg aatgaggaga gaagaggaag
      601 cagaaacatt tagaactact ttttgaaata actctaaggc tgcggtataa ttttctttct
      661 ttaaaaaaca atatgcagca ttgtaacggg cattattagt atttatttct tcgtttgttt
      721 gagggttttt taaatataaa gtaaaataat ctagagcttc attggtgttg ccatttctat
      781 aagcaatttc tcctttccag aaataagtat attggatata atttgcatta aatggtactt
      841 gtaaaagttt tgataataat tcattagcta caactaactg ttggtcgtta atattttcta
      901 cagctctgcc atataaaatt ttagggtaaa ttttttgaat attttcactt tgttgcggca
      961 gagaatctat taaagccatt gcttctctgt aattacttgt atttgccaaa atggctatct
     1021 gtagttcttt tgcttcgttt aaataaacag attttgggta tgtttttaga aaagatttta
     1081 tttctttatg ggcaatatct aaatagcctt gctcgtaact aagcttagca taattaaact
     1141 gcgatacctc tttttgtaca gcgttactac tgttggttgc acaaaataaa aaagcatttc
     1201 ttgctccaac tttatcgtca atttttaaat aggcatcagc taaaagatac atgctgtttt
     1261 gagctaaaga atcttcttta ccacctaatt gtttaaaacc atcaatagct ttattccatt
     1321 taccagcttc gtaataacag tatgctaatt cgtataaatc ttctcttctt acttttttag
     1381 attttgttac atattcttct aagtagggta gtgcctcttt gtacattctt ttctcaaaca
     1441 aagagtgccc aataagttgt gttaattcta atttataata ctgatttcct ttttgtagtg
     1501 cagttttagc atattcaata gccttgtctc tttctccagc aaaataataa atctcggcaa
     1561 tataaaatgg cactatgttt tgataggttt ctgttttttc aactagttta aagctttcta
     1621 atgcttcttt atatttcttt tcaaaaaaca ttataaagcc gtagtaataa tttgcatcta
     1681 tataatttgg gctttctgta agttgcctaa ttgaattaaa taaaggttta gcttttacaa
     1741 aattttgagt ggtaaaataa ccataagcct gatgaaattt catttgggct atttgttggt
     1801 tggttaggtt ttctatgctt gctttctcaa aataattatt agctgtattg aaatcttttt
     1861 ttctgtaata atattcgcct aaataaaaac ttacaatgcc taccttttga gcattggctt
     1921 ctaaagtaat aaaatcaact gctaaagttt caatggttgg atcgttaagc tgtaatccgc
     1981 aaataataaa gtaaaatttt gattcggttt ttatatgttc ttttaaattg ctattatagt
     2041 tatcttgagt ataaatattt ttaaaaattg gataggcaag gctgtattgc tctttttgcc
     2101 aatattcttt agccatttta taagcaatat cagaatcggt attaattttt gtgaattggg
     2161 cattgccagt aagagctata cctacaatag ctaaaataat taatagcttc attttatgtt
     2221 gatgcataga ataataaact tatgaataac aaatattgtt ttttattttc ttaacaaagt
     2281 gcaaaatcgg ttgtttatgc aaagtgtaca agtgcctttt gtacccatta tttattattt
     2341 gtggataaac tgtataatgt ataaatttta taaaccataa atcaaagtat aaatagtaat
     2401 tactttattt ttggtacata taccaataca atgattcaac aattttctta ttttatacaa
     2461 gatgtacaac gaatgttggg aaagtataaa attagaatta tacatatatg gcttagccga
     2521 cttttttggg gagttttttt atataggtta
//
LOCUS       DAOQPK010000286         2493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_34723_length_2493_cov_4.157506, whole genome shotgun sequence.
ACCESSION   DAOQPK010000286 DAOQPK010000000
VERSION     DAOQPK010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34723_length_2493_cov_4.157506"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PLU36_10260"
     CDS             <1..409
                     /locus_tag="PLU36_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HMZ47176.1"
                     /translation="LHLRMQRHCENGEKIAHWLRKHPKDKQVYWPGFEDNKNYSIAKS
                     QMNGFGGMISFELVNESVEEAKRVLSSTHLFSLAESLGGVESLINHPASMTHASIPRE
                     ERIKNGLSDGLIRLSVGIEDADDLINDLDKAIG"
     gene            complement(418..2397)
                     /locus_tag="PLU36_10265"
     CDS             complement(418..2397)
                     /locus_tag="PLU36_10265"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HMZ47177.1"
                     /translation="MLIKWKVPKTIQWVIKLFFIFLFIFTLFRISTYIAFKPDDIKLN
                     DVWSCFAMGLRFDIRWISILLIPIVLISLIPNFSPYSSTTTKRFWIVYLAICTFLLMF
                     FFGADYGHFSYVSTRLNASALNFAEDAKISFEMLWESYPIFWMILALIILAFLFYKLY
                     TLTHQRIEANNITSINYLRRWYLVISLFLIWGIYGSINSKPLKWKDAFIYKNNFKSYL
                     ALNPLQNFFTTLRFRKPVADGNLVHKYYNDIANFLQFDSLTKQQASFKRWIYPHSHAL
                     ESKPNVVLVICESFSMYKSSMSNNPLNATPYFNTLSKQGIFFNRCFTPHFATARGIFA
                     TLTGIPDVQLSKFSSRNEESLSQHTIINNFDDYNKFYFIGGSSEFNNYKGLLKNIKNL
                     QLFEEGSYTSPKVNVWGISDKDLFTESNNILAKQEKPFFAIIQTAGNHRPYTIPENDK
                     EFKRKIIDKDTLQKYGFLSEEEYNAFAYMDYSIEHFIKTATKEKYFQNTIFVFVGDHG
                     VEGNASAMYPNAWTINRLSDEHVPLLFFAPHLLQPKQYQKVVSQIDVLPTIAGLVYQP
                     YFNTTLGRDILKPGIKNNGAFIIHHDEGKIGWLTDSFYFVKNIRFEQEELIPIQSNIA
                     NWSPTQETEIKKKYSVFTSAFYETAKWMLINNKQK"
BASE COUNT          875 a          364 c          375 g          879 t
ORIGIN      
        1 tttgcattta cgcatgcaac gccattgtga aaatggagaa aaaattgctc attggttacg
       61 caagcatcca aaggataaac aagtttattg gccaggattt gaagataata aaaactatag
      121 cattgctaaa agtcaaatga atggttttgg tggaatgata agttttgaat tagtaaacga
      181 aagtgtagaa gaggctaaaa gagttttatc cagcacacac cttttttcat tagcagaaag
      241 tttaggaggt gtagaaagtt taattaacca tcctgcaagt atgacacatg caagtatccc
      301 aagagaagaa agaattaaaa atggtttatc tgatggatta attagattaa gtgttggtat
      361 tgaagatgca gatgatttaa ttaacgattt agataaagca attggatagt taataaatta
      421 tttctgttta ttattaatca gcatccattt tgctgtttcg taaaaagcag atgtaaaaac
      481 agaatatttc ttttttatct ctgtttcttg tgtgggtgac cagtttgcaa tattagattg
      541 aataggaatt aattcttctt gttcaaatct tatgtttttt acaaagtaga aagagtctgt
      601 tagccagcca atttttcctt catcgtgatg tataataaat gctccattgt tttttatacc
      661 tggttttaag atatctctgc ctaatgttgt attaaaataa ggttgatata ctaaacctgc
      721 aatagttggt aatacatcta tttgtgaaac aactttttga tattgtttag gctgtaacaa
      781 atgtggtgca aaaaataata gaggtacatg ctcatcgcta agcctgttta ttgtccatgc
      841 atttggatac attgcagaag cattaccttc cactccatga tctcctacaa aaacaaaaat
      901 agtattttga aaatattttt ccttagtggc agttttaata aaatgttcta ttgaataatc
      961 catataagca aaagcattgt attcctcttc cgataaaaat ccatacttct gaagtgtatc
     1021 tttatcaata atttttcttt taaattcttt atcattttct ggaatggtat aaggcctgtg
     1081 gttgccagca gtttgaatga tagcaaaaaa tggcttttct tgctttgcta aaatgttgtt
     1141 actttcagta aataaatctt tatcgctaat accccataca tttacttttg gcgaggtata
     1201 acttccttct tcaaacaatt gtaaattttt tatatttttt aataaccctt tgtagttatt
     1261 aaattcacta ctgcctccaa taaaataaaa tttattatag tcatcaaaat tattaataat
     1321 tgtatgctgg cttaggctct cttcatttct actactaaac tttgataatt gtacatctgg
     1381 tatgcctgta agtgttgcaa aaatgccacg tgctgttgca aaatgtggtg taaaacatct
     1441 attaaaaaaa ataccttgtt tgcttaaagt gttaaaataa ggtgttgcat ttagcggatt
     1501 attactcatg ctacttttat acatactaaa actttcgcaa ataaccaaca ccacatttgg
     1561 tttgctttct aatgcatggc tatgtggata tatccacctt ttaaaactag cttgctgttt
     1621 tgttaagcta tcaaattgta aaaaattagc tatatcgtta taatatttat gaacaagatt
     1681 accatcggct acgggttttc taaaacgaag tgtagtaaaa aaattttgta aagggtttag
     1741 tgctaagtac gacttaaaat tatttttata tataaaagca tctttccact ttagaggctt
     1801 gctgttaatg ctaccataaa tgccccatat taaaaaaagt gaaataacca aataccatct
     1861 tcttaagtag tttatgcttg ttatattatt tgcctcaata cgttggtgtg ttaaagtata
     1921 taatttataa aacaaaaaag ccaatataat taaagccaaa atcatccaaa aaattggata
     1981 cgactcccaa agcatttcaa aagaaatttt tgcatcttcg gcaaagttta atgcacttgc
     2041 atttagcctt gtactaacat aagaaaaatg tccataatct gcaccaaaga aaaacattag
     2101 taaaaaagta caaattgcca aatacactat ccaaaaccgt ttggtagtag tagagctgta
     2161 aggagaaaaa ttaggtatta aactaattaa tacaattgga attaataaaa tgcttatcca
     2221 gcgaatatca aatcttaaac ccattgcaaa gcaactccaa acatcattta gcttaatatc
     2281 gtctggttta aaagctatat atgtagaaat tctgaaaagt gtaaatatga ataaaaaaat
     2341 aaaaaataat tttataaccc actgtattgt tttgggaact ttccacttaa taagcatgat
     2401 tatgggtttt gctttttatt atttaataac atataccttg ctgtttcata aaaagcatct
     2461 gtaataaaat gtaattgctt tttaatacta tcg
//
LOCUS       DAOQPK010000287         2487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_34878_length_2487_cov_3.865543, whole genome shotgun sequence.
ACCESSION   DAOQPK010000287 DAOQPK010000000
VERSION     DAOQPK010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34878_length_2487_cov_3.865543"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="PLU36_10270"
     CDS             <1..892
                     /locus_tag="PLU36_10270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ47178.1"
                     /translation="NMNLFTTNTTNIADLIPTPATGLTILGKDSICSSEIYTVNVPTG
                     TSVSFTVSVSPNSSYASYSASGNEISVTRNGSNIGTVKITATVTDDCGNQYEVNRTVY
                     LGPIPAVIMGPYDPVEHTVMGVACVGEEYYFAPYSIDPTATYTWKFIPPVWSQDWEPT
                     YLASGPYAYFTMPDTPRCYTVRGEKTNACGGTVVTQKKICVVECNAMRLLASPNPATS
                     LLTVTLIDEKEKISKQIKQENIQIEFYNLNVGIKQKEWKYNTTAQRQFTLNIADMAKG
                     MYVVKVTKGKLQSTTKIIKD"
     gene            1054..1602
                     /gene="rfbC"
                     /locus_tag="PLU36_10275"
     CDS             1054..1602
                     /gene="rfbC"
                     /locus_tag="PLU36_10275"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008581998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HMZ47179.1"
                     /translation="MKIETTPLKDCFIIHDTIIKDSRGYFFESFNKRKFKELTNIEID
                     FVQDNQSQSSKGVLRGLHYQKDMYAQAKLVRVLKGKVLDIAVDLRKNSPTFGQHFAVE
                     LSEDNNLQFFIPRGFAHGFIVLSDETVFFYKCDNYYNKEYDTGIKFNDPTLQINWLLK
                     EEEIIVSEKDANLPLFKDAVYF"
     gene            1633..>2487
                     /gene="rfbA"
                     /locus_tag="PLU36_10280"
     CDS             1633..>2487
                     /gene="rfbA"
                     /locus_tag="PLU36_10280"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HMZ47180.1"
                     /translation="MKGIILAGGSGTRLYPITKGISKQLMPIYDKPMIYYPLSVLMLA
                     GIKEVLIITTPEDSSQFQRLLGTGEELGCVFSYAVQHTPNGLAQAFIIGADFVGSDKV
                     ALVLGDNIFYGSGFSKLVQSFNNVTGAAVFAYEVNDPERYGVVEFDEKFKAISIEEKP
                     IKPKSNYAVPGLYFYDNSVIEIAKNIAPSARGEYEITDVNRVYLEKGNLQVGVMSRGT
                     AWLDTGTFDSLSDASEFVRVIEKRQSQKIGCIEEVAYRMGFINHQQLMTLANKYAKSG
                     YGEYLRKVK"
BASE COUNT          920 a          352 c          451 g          764 t
ORIGIN      
        1 taatatgaac ttatttacta caaatacaac caatattgca gatttaatcc ccacacctgc
       61 tacagggttg actatattgg gtaaagacag tatttgttca tcagaaatat atacagtaaa
      121 tgtgccaaca ggtacatctg taagttttac tgtttccgtt tccccaaatt caagctatgc
      181 atcttatagt gcaagtggta atgaaatatc agtaacacgc aatggatcta atataggtac
      241 ggtaaaaata actgctactg taactgatga ttgtggtaat cagtatgaag taaacagaac
      301 tgtttatctt ggaccaattc ctgctgttat tatgggtcct tacgaccctg tagaacatac
      361 tgttatgggc gtagcctgtg tgggtgaaga atattatttt gctccctatt ctatagaccc
      421 tacagcaact tatacatgga aatttatacc tcccgtatgg tctcaggatt gggaacccac
      481 ttatttagct tcgggtcctt atgcttattt taccatgcca gatacacctc gttgttatac
      541 tgtaagagga gaaaagacaa atgcctgtgg aggtactgta gttacacaaa aaaagatttg
      601 tgttgtagag tgtaatgcca tgaggctatt agcctctcct aaccctgcta caagtttgct
      661 gacagtaacc cttatagatg aaaaagaaaa aattagcaaa caaataaagc aggaaaacat
      721 acaaatagaa ttttataatc ttaatgtagg tattaagcaa aaagaatgga aatataatac
      781 cactgctcaa cgacagttta ccttaaacat agcagatatg gctaaaggta tgtatgtggt
      841 aaaagttaca aaaggcaaac tgcaatcaac cacaaaaatt ataaaagatt aagaaagtga
      901 tttatttaat gagaagtagc aatatgccaa atagaaatat tatcttggta gtagtggaat
      961 ttaaagaaaa gggttaaggt tatttagaac agaaaatacc taaccaaaac taaattttat
     1021 cacaaccaat ataaagccaa ttaattaact aaaatgaaaa tagaaaccac gcctttaaaa
     1081 gattgtttta ttattcacga tactattata aaagatagta gaggctattt ttttgaaagt
     1141 tttaataaaa gaaaatttaa agaattaact aatatagaaa tagattttgt acaagacaat
     1201 caatcccaat cttctaaagg agttttacga ggtttgcatt accaaaaaga catgtatgct
     1261 caagctaaat tggtaagagt attaaaagga aaagtattgg atattgctgt agatttaaga
     1321 aaaaactcac ccacatttgg gcaacatttt gcagtagaat tatccgaaga caataattta
     1381 caattcttta ttccaagagg ctttgctcat ggttttattg tattgagtga tgagacagtt
     1441 ttcttttata aatgcgataa ttactacaac aaagaatatg acactggcat taaatttaac
     1501 gaccccactt tacaaataaa ttggttatta aaggaagaag aaattattgt ttctgaaaaa
     1561 gatgcaaacc ttcctttatt taaagatgca gtatattttt aaaactaaat ttgctcaaaa
     1621 aataatagta caatgaaagg aattatttta gcaggaggta gtggtacaag gctttaccct
     1681 attaccaaag gaattagtaa gcaactaatg cctatttacg ataagcccat gatttattat
     1741 cccttatcag ttttaatgtt agctggtatt aaggaggttt taattattac cacacccgaa
     1801 gattcttcac aatttcaacg tttgttaggt actggcgaag aattaggttg tgtattttct
     1861 tatgctgtac aacatacacc caatggttta gcacaggcat ttataatagg tgcagatttt
     1921 gttggtagcg ataaagtagc attggtatta ggtgataata ttttttatgg ttctggcttt
     1981 agtaaattag tacaatcttt taacaatgta acaggggcag cagtttttgc ttatgaagtt
     2041 aatgaccctg aaaggtatgg tgtagttgaa tttgatgaaa agtttaaagc aatatccatt
     2101 gaagaaaaac ctataaaacc aaagtctaac tatgccgttc caggattata tttttatgat
     2161 aactcagtaa tagaaattgc aaaaaatatt gcaccaagtg caagaggtga atatgaaatt
     2221 acagatgtaa acagagtgta tttagaaaaa ggaaatttac aggtaggtgt aatgagcaga
     2281 ggaacagctt ggttagatac aggaactttc gattcattaa gcgatgcatc agagtttgta
     2341 agagtgatag aaaaaagaca aagtcaaaaa attggatgta tagaagaagt tgcttatcgc
     2401 atgggtttta tcaatcatca gcaattaatg acattagcga ataaatatgc aaaaagtggc
     2461 tatggagagt atttgagaaa agtgaag
//
LOCUS       DAOQPK010000288         2477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_35118_length_2477_cov_4.451693, whole genome shotgun sequence.
ACCESSION   DAOQPK010000288 DAOQPK010000000
VERSION     DAOQPK010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35118_length_2477_cov_4.451693"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PLU36_10285"
     CDS             <1..608
                     /locus_tag="PLU36_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HMZ47181.1"
                     /translation="QKKARIMWIIAGIIFRCSLLLALQWLLDQKGKAIFTIYEKPFDL
                     ASLVMLAGGLFLMYKSVKEIHHKVEGEEDATKQNNKPLSFTNAIFQIMIIDIVFSFDS
                     IITAGGTAKHIEIMIAAVVIAMVIMFIFSTNIANFIQQHPTIKMLALSFLVMIGLSLI
                     IEGWNPVAAHDLHLKNYIYFAMAFSFAVELLNMRMRKKVKL"
     gene            complement(598..1200)
                     /locus_tag="PLU36_10290"
     CDS             complement(598..1200)
                     /locus_tag="PLU36_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HMZ47182.1"
                     /translation="MNYFTHLKKDKTLAICLLNEMPTLSFQKNIPLRLIAAIMSQQLS
                     TKVAKIIYQRFLDLYNGKVPSPQMVLDTPFATLRNIGLSNAKVNYVHNVATFCIENKI
                     TDKKLQKLSNEEIIELLIKIKGVGKWTVEMLLMFSLGREDVFSADDLGIQQAMIKLYK
                     LDTTNKKLLLANMQKIAEKWKPYRTYACLHLWRWKDTVKA"
     gene            1407..>2477
                     /locus_tag="PLU36_10295"
     CDS             1407..>2477
                     /locus_tag="PLU36_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase SNF2"
                     /protein_id="HMZ47183.1"
                     /translation="MALPYLINHIYNNGSEEVVRRGKKIHQSGNVELIENNEFLGNLT
                     FRIKDDTYHTFYKVYITNFKDEKNIGTRCTCPYNLSEICRHKAGTLFYLQELLDKNLL
                     GSTSRAYNQQHTILKMKQLDLKFIKMLSSSKAFADAEEFLRSKKANIIEAKDECVLAE
                     VEWNNKTWKVIIRKNEERNFDTSCTDTSETNYPLCTQKIIVLLQLLNRYGSNYFDSIR
                     NWDAVKNKLLGIYGYTLSDDLTNKFEFTYKDGKPFLRVLDQNIKRIDAPLNAEIKPKP
                     SWKETYGETKNEPIETEETLKKNIEQNSTIKLGIVISNNNSQYPFFQIDAIQGEAAED
                     YSSYVGKVEKLDLNKFVDTTLFN"
BASE COUNT          935 a          343 c          364 g          835 t
ORIGIN      
        1 tacaaaaaaa agcaagaata atgtggatta ttgcaggtat aatttttaga tgctctttac
       61 ttttggcttt gcaatggctg ttagaccaaa aaggcaaagc tatttttacc atatatgaaa
      121 aaccttttga tttagctagt ttagtgatgc ttgcgggtgg tttattttta atgtacaaat
      181 cggttaaaga aattcatcat aaagtagagg gtgaagaaga tgcaacgaag caaaataata
      241 aaccactatc ttttactaac gcaatttttc aaataatgat tattgatatt gtttttagtt
      301 ttgatagtat tattactgca ggcggtactg caaaacatat tgaaataatg attgctgctg
      361 ttgtaattgc aatggttata atgtttatat ttagtaccaa tattgcaaat tttattcaac
      421 aacatccaac cataaaaatg cttgccctat cttttttggt aatgattggt ttatctttga
      481 ttatagaagg ttggaaccca gtagcagcac acgatttaca tttaaaaaat tatatttatt
      541 ttgctatggc tttctccttt gctgtagaac ttttaaatat gagaatgcga aaaaaagtta
      601 agctttaact gtgtctttcc atctccacaa atgcaaacaa gcataagttc tataaggctt
      661 ccatttttca gcaatttttt gcatattagc aagtaatagt tttttatttg ttgtatctaa
      721 tttatacaac ttaatcatag cctgttgaat gcccaagtca tctgcagaga aaacatcttc
      781 tctgcctaag ctaaacatta acaacatttc tactgtccat ttaccaacac cttttatttt
      841 aataagtaat tcaataattt cctcgttgct cagtttctgc aatttcttat ctgtaatctt
      901 attttcaata caaaaagtag caacattgtg tacataattt actttagcat tgcttaatcc
      961 aatatttctt aaagtggcaa aaggagtgtc taaaaccatt tgagggcttg gtactttacc
     1021 attatataaa tctaaaaatc tttgataaat tattttggca accttagtac ttagctgctg
     1081 gctcattata gcagcaataa gccttaatgg tatatttttt tgaaaagata gtgttggcat
     1141 ttcattcagt aaacaaatag ctagagtttt atcttttttt aaatgtgtga aataattcat
     1201 ctttaaaatt gagtgatggg aaaaagtata tatcaaaatt tatggataaa aaaaaacggc
     1261 acaagttaca tctttttagc aaataatcct taataataac tgaataaaat atttcttcca
     1321 caaactatta agtatagagt aaaataaggg taacattctt ttatttttgc tacttaacct
     1381 tttctattaa tccacatttt tcatcaatgg ctctacccta tttaattaat cacatttaca
     1441 ataatggctc agaggaagtt gtaagaagag gtaaaaaaat tcatcaatct ggcaatgtag
     1501 aacttataga aaacaatgag tttttaggaa atttaacttt tagaataaaa gacgatacct
     1561 atcatacttt ttataaagta tatattacca attttaaaga tgaaaaaaat attggtacaa
     1621 gatgtacttg cccttataat ttatctgaaa tatgcagaca caaagcaggt acattatttt
     1681 atttacaaga attattagat aagaacttgc taggctctac aagcagagct tataaccaac
     1741 aacatacaat tttaaaaatg aagcagcttg atttgaagtt tatcaaaatg ctgtcttcat
     1801 caaaagcatt tgccgatgca gaagaatttt taagaagtaa aaaagcaaat attatagagg
     1861 caaaagacga atgtgtttta gcagaagtag aatggaataa caaaacatgg aaggtaataa
     1921 taagaaaaaa cgaagaaaga aattttgata caagttgtac agatacaagt gaaaccaact
     1981 acccattatg tacacaaaaa ataattgttt tattacagtt attaaacaga tatggtagta
     2041 attattttga tagtattaga aactgggatg cagtaaaaaa taaacttctt ggtatttatg
     2101 gttacacttt atcagatgat ttaacaaaca agtttgaatt tacttataaa gatggaaagc
     2161 ctttcttaag agtattagac caaaatatta aaagaattga tgcaccttta aatgcagaaa
     2221 taaaacctaa gccatcttgg aaagaaactt atggcgaaac taaaaacgaa cccatagaaa
     2281 ctgaagaaac cctgaaaaaa aatatagaac aaaattctac cataaaatta ggtatagtaa
     2341 tatccaataa taattcacaa tacccatttt ttcaaataga tgccattcaa ggcgaagctg
     2401 cagaagatta tagtagttat gtagggaaag tagaaaaatt agacttgaat aaatttgtgg
     2461 ataccacttt atttaac
//
LOCUS       DAOQPK010000289         2449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_35838_length_2449_cov_3.500418, whole genome shotgun sequence.
ACCESSION   DAOQPK010000289 DAOQPK010000000
VERSION     DAOQPK010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35838_length_2449_cov_3.500418"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PLU36_10300"
     CDS             <1..257
                     /locus_tag="PLU36_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HMZ47184.1"
                     /translation="IVRKELLIPIFCGIFLVGNLSVIIQVSYFKYTKKKYGEGRRVFL
                     MSPLHHHYQKRGYHENKIAIRFWIITVLLVIASIATLKMR"
     gene            339..1679
                     /gene="murD"
                     /locus_tag="PLU36_10305"
     CDS             339..1679
                     /gene="murD"
                     /locus_tag="PLU36_10305"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HMZ47185.1"
                     /translation="MAHRIVILGAGESGVGAALLSKKKGYEVFVSDAGVIKEKYKKKL
                     FDNEIEFEEGKHTAEIILNANEVLKSPGIPETNKMVIAIREKGIEVIGEFELAYRCKG
                     DSKIVAITGSNGKSTTTALIYHICQKAGLDCALVGNIGYSIAAQIAEDPKMLYVAEIS
                     SFQLDDIKTFRPDIAVLLNITEDHLDRYNYQFENYINSKFRVILNQNKNDYFIYNDDD
                     EIITKKLKTLIPNTNPLPFSMKHEIKKGGNIKGDQMMLRIQEERVSMSIYDFALKGKH
                     NNYNTMAAGIAAATLGIRKDKIREAVKDFQSLEHRMEFVATVRGVEFINDSKSTNTNS
                     TWFALESMNKPTVLILGGVDKGNDYELMNELVAQKVKAIVCLGNDNKKILEAFKDKVP
                     VIVEADSAKKAVNAAFKLSVKGDVVLLSPACASFDLFKNYEDRGVQFKEAVKEL"
     gene            1723..>2449
                     /locus_tag="PLU36_10310"
     CDS             1723..>2449
                     /locus_tag="PLU36_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HMZ47186.1"
                     /translation="MFNKSNIPFSKTSPLSKLSKADIPFASLNKESLLKRTSGDKYIW
                     GIVLVLALISILVVYSATGSLAYKMNKGNNEVYLFKQLSFTVIGLMVMYFLHRINYTL
                     FSRIATLLFFISIPLLIYTLVFGAKINEGSRWIKLPVINMTVQTSDIAKLALFMYISK
                     LLSKKQEVIKDFKKGFLPIITPVFVICVLIMPANLSTALLTGATSLLIMFIGRISLKH
                     ILLTILVASIPILFIVSVAWLTYN"
BASE COUNT          932 a          311 c          445 g          761 t
ORIGIN      
        1 ttattgtaag aaaagaatta ttaattccaa tattctgcgg aatatttctt gttggaaatt
       61 taagcgtaat cattcaggtt tcttatttca aatataccaa aaagaaatac ggagaaggaa
      121 gaagggtttt tttaatgagc cctttacatc atcattatca gaaaagaggg tatcacgaaa
      181 ataaaattgc aataaggttt tggataatta ctgtgttatt agttattgct agtatagcaa
      241 cattaaaaat gcggtaaaag ctgtaagtga acttttaaag taggagttaa aatttactgc
      301 ttgttgaaag aaaaatatta aagagaattt aaaaaaatat ggcacataga atagtaatac
      361 ttggggcagg agaaagtggt gtaggtgctg cattattaag taaaaagaaa ggatacgaag
      421 tttttgttag cgatgctgga gtaataaaag aaaaatataa aaaaaagctc tttgataatg
      481 aaatagaatt tgaagaagga aaacatactg ctgaaattat attaaatgcc aacgaagttt
      541 tgaaaagccc gggaattcct gaaactaata aaatggttat tgctattaga gaaaaaggaa
      601 tagaagtaat tggtgaattt gagttggcat atagatgtaa aggagatagc aaaatagttg
      661 caattacagg tagcaatggt aaaagcacaa caactgcact tatttatcat atctgccaaa
      721 aggcaggatt ggattgtgca ttggttggta acattggtta cagcattgca gcacaaattg
      781 cagaagatcc taaaatgttg tatgtagccg aaatcagttc atttcagtta gatgatatta
      841 aaactttcag gccagatata gctgtgttac taaacattac agaagaccat ttagacagat
      901 ataactatca gtttgaaaac tatataaaca gtaagtttcg agtcatcttg aatcaaaaca
      961 aaaatgatta ttttatttat aatgatgatg atgaaataat aaccaaaaaa ttaaaaacat
     1021 taatcccaaa tactaaccca ttaccatttt ctatgaaaca tgaaataaaa aaaggaggca
     1081 acattaaagg agatcaaatg atgctccgca ttcaggagga aagagtaagt atgagtattt
     1141 acgactttgc tctaaaaggt aaacacaaca actataacac catggcagca ggtattgcag
     1201 cagctacact cggaattaga aaagataaaa taagagaagc tgtaaaagat tttcaaagcc
     1261 ttgagcatcg tatggagttt gttgctactg ttcgtggtgt agagtttatc aatgatagta
     1321 agtcaacaaa taccaacagt acctggtttg ctttagaaag tatgaacaaa ccaacagtac
     1381 ttattttagg tggtgttgat aaaggcaatg actacgaatt aatgaatgaa ttagtagccc
     1441 aaaaagtaaa agctattgtt tgcttaggca acgataataa aaaaattttg gaggcattta
     1501 aagataaagt acctgttata gtagaggcag actcagcaaa gaaagctgtt aatgctgcat
     1561 ttaagcttag tgtaaaaggc gatgtagttt tattaagtcc tgcatgtgca agctttgatt
     1621 tgtttaaaaa ctacgaagac agaggagttc aatttaaaga agctgtaaaa gagctatagt
     1681 atttatcaaa caataaaagc aaaaatagaa taacaagaat taatgtttaa taagtctaac
     1741 ataccatttt ctaaaacctc tccactttct aaattaagca aagcagatat tccatttgct
     1801 tcactaaaca aagaaagttt actaaaaaga actagtggag acaaatacat ttggggaatt
     1861 gtattggtat tagcacttat ttccattctt gtggtttata gtgcaaccgg atctttggca
     1921 tataaaatga acaaaggcaa taatgaagtg tatttgttta aacagttatc ctttacagta
     1981 atagggttga tggtaatgta tttcttgcat agaataaatt atacgctttt ttcaagaatt
     2041 gcaactttgt tattctttat ctctattcct ctgttaattt atacattagt ttttggagca
     2101 aaaattaatg aaggtagtag gtggataaag ctacctgtaa ttaatatgac tgttcaaacc
     2161 agcgacattg ctaaacttgc tttgtttatg tatatttcaa agctattaag caaaaagcaa
     2221 gaagtaataa aagattttaa gaaagggttt ctgccaatta tcacaccagt gtttgtaatt
     2281 tgtgttttaa taatgcctgc aaacttaagt acagctttgc ttacaggggc aacttcttta
     2341 ttaattatgt ttataggtag aattagttta aagcatattt tactaaccat attagttgca
     2401 tctataccaa tattatttat agtatctgtt gcttggctta cctacaata
//
LOCUS       DAOQPK010000290         2432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_36316_length_2432_cov_4.724022, whole genome shotgun sequence.
ACCESSION   DAOQPK010000290 DAOQPK010000000
VERSION     DAOQPK010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36316_length_2432_cov_4.724022"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="PLU36_10315"
     CDS             complement(<1..750)
                     /locus_tag="PLU36_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47187.1"
                     /translation="MKKIYLLIPVLLFIKINLHAQLNYSFSATSSSYSAIVGGANPTL
                     LATTSGGKTDEGYANNISIGFTFNYNGTNYTTLGICTNGFIYLGGSLTNSSITYVNNI
                     ATEMAGVRPIIAPLWDDIDVQTETNIKYLVTGTAPNRVLTIEWANALWDPFAASAAIS
                     FQVKLYETSNIIEFVYKQEAGSIENIFGGVGASIGITNGNTGDGNYLSLNNSSNNPVV
                     SSLVNTNSINTKPATGQLYRFTPTDSCNGQPS"
     gene            complement(1205..1327)
                     /locus_tag="PLU36_10320"
     CDS             complement(1205..1327)
                     /locus_tag="PLU36_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47188.1"
                     /translation="MKTSIVKILLVVLVLSTVASSCSIFGKGRKDKSCMCPNYR"
     gene            1379..>2432
                     /locus_tag="PLU36_10325"
     CDS             1379..>2432
                     /locus_tag="PLU36_10325"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ47189.1"
                     /translation="MNKKFNIQTVLVAPLDWGLGHATRCIPIISALLKNNYTVILAAS
                     GKQKILLQQEFPEIKIIDLKGYNIQYSKTSFGLPFKIFLQIPKIVWAIYYEHKWLKKI
                     VNKHAIDCVISDNRYGLFSKKIPTVFITHQLTIKMPFLWLQSFVQKINYKFINQFTAC
                     WIPDVEGKDKMAGILSHPTKYPSMPIQYLGNLSRFEWQNTVEAYTYKYCILLSGPEPQ
                     RSILEKIIIKDLPNITQQVLLIRGLPNDNTVLPTNNNVIVKNHLQGVEICKALQQSEW
                     VICRSGYTTIMEILSLRKKAVLIPTPAQTEQEYLANFLMQKQWCYSVAQNKFVLSKER
                     NNIENFSFHVPVIGANN"
BASE COUNT          859 a          369 c          354 g          850 t
ORIGIN      
        1 actaggttgc ccattacaag aatctgttgg ggtaaacctg tataactgcc cagtggcagg
       61 cttagtatta atactattgg tgtttaccaa actacttaca acagggttat tgctgctgtt
      121 atttaacgat aaataattac catctcctgt attgccattt gtaattccaa tagaagcacc
      181 aacaccgcca aaaatatttt ctatactacc tgcttcttgc ttatatacaa attcaattat
      241 attgctagtt tcatatagct ttacttgaaa agaaattgct gcagatgctg caaatgggtc
      301 ccatagggca ttagcccatt caatagttaa cactctatta ggtgcagttc ctgtaactaa
      361 atacttaata tttgtttctg tttgtacatc tatatcatcc cataaaggtg caataattgg
      421 tctcactcct gccatctctg ttgcaatatt attaacataa gttatactgc tattggttag
      481 gctgccacct aaataaataa aaccattagt acaaatacct aaggtggtat aattagtacc
      541 gttataatta aaagtgaaac caatggatat attgttggca taaccttcat ctgttttacc
      601 accagatgta gtggctaata acgtagggtt tgcaccacca acaattgcac tataactact
      661 gcttgtggca ctaaagctat aattaagttg tgcatgaagg tttattttaa taaacaataa
      721 aacgggtatt aaaaggtaaa tttttttcat ctttaaaatt ttctaattaa tgtacttatt
      781 aaagaattat tagttgaaat atttagtaaa gctattattt ttttagaatg taaagtaata
      841 agatgtttcc aaactgcata cagcattaac atggtaaagc tttggttctt taaatacgca
      901 ttcaagtttt gttataatta gttggtgttt tttagataaa tagataagca ataaaagcaa
      961 ctaaaagata taataatttg taagaaaaca aattggaaaa taatttttaa gcataaaaag
     1021 atttttgttt tttactttat tgatgagttg ctactaccaa caaaactaac attaaggggg
     1081 ctatatattt ttttaaaaat ccaactttag gttgcatttt tacttacaaa taattgtgtt
     1141 aaactgtaac aagttttaat taatgtaaat gtaaaaaagt atccctagca tttacttcaa
     1201 tatattatct gtaattaggg cacatgcaag atttgtcttt tcttccttta ccaaatatac
     1261 tgcaagaact tgcaactgta cttaaaacta atacaaccaa tagtattttt acaatgcttg
     1321 ttttcataat ggcgtgtaaa ttaatattta atttgtaaat aattactttt atggcctgtt
     1381 gaataaaaaa tttaacatac aaactgtttt ggtagctcct ttagattggg gcttaggcca
     1441 tgctacaagg tgtattccta ttattagtgc tttactcaaa aataattata ctgtaatact
     1501 tgctgccagt ggcaagcaaa aaattttatt acaacaagag tttccagaaa taaaaattat
     1561 tgacttaaaa ggctataata ttcagtacag caaaacttct tttggcttac catttaaaat
     1621 ttttttacaa atacctaaaa tagtatgggc tatttattac gagcataaat ggctaaaaaa
     1681 aattgtaaat aagcatgcca tagattgtgt aataagcgat aatagatatg gtttattttc
     1741 aaaaaaaata cccacagttt ttattactca tcagcttacc attaaaatgc cttttctatg
     1801 gttgcaatct tttgtgcaaa aaattaatta taagtttata aaccaattta cggcttgttg
     1861 gatacccgat gtagaaggta aagataagat ggcaggcatt ttatctcacc ctactaaata
     1921 tccttccatg cctatacaat acttgggtaa tttatctagg tttgaatggc aaaatactgt
     1981 tgaagcctac acatacaaat attgtatttt actttctggg cccgagccac agcgttctat
     2041 tttagaaaaa attattataa aagatttacc caatatcact caacaagtat tattaatacg
     2101 tggcttgcct aatgataata cagttttacc aactaataac aatgtaatag ttaaaaatca
     2161 tttacaaggg gtagaaattt gtaaagcctt acaacaaagt gagtgggtta tttgccgtag
     2221 tggctacaca accattatgg aaatattaag tcttagaaaa aaagctgtat taatacccac
     2281 acctgcacaa acagagcaag aatatttagc caatttttta atgcaaaaac aatggtgtta
     2341 ttctgttgct caaaataaat ttgtattaag taaagagagg aataatatcg aaaatttttc
     2401 ttttcatgtt ccagttattg gtgctaataa tt
//
LOCUS       DAOQPK010000291         2431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_36347_length_2431_cov_3.697391, whole genome shotgun sequence.
ACCESSION   DAOQPK010000291 DAOQPK010000000
VERSION     DAOQPK010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36347_length_2431_cov_3.697391"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1293)
                     /locus_tag="PLU36_10330"
     CDS             complement(<1..1293)
                     /locus_tag="PLU36_10330"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMZ47190.1"
                     /translation="MQISKTKQIVLIAILLINYSTIKAQTKHSFTITQTVEYASKNNA
                     QVKNALLGILAQEQVNREVTANAYPTIGADITVNNNIVVSTQVIPGDMFGQPGQLVPV
                     KFGIKYNSNAMIQLRQLLFDGQVFVGLQARKASMEFAKKNAEVVVENIKVNIYKIYYQ
                     LVVSKTQIELLNTNIERIEKLKHDANQLYINGFAEKLDVDKIDVTLTNLKTERVKALN
                     NIEIGYLGLKTLIGMPLNDTLELTDKITDNDIKQNALTDSINFNDRKDFQYLQTVKKL
                     NEYNIKRYKLSYLPTVAISGVYGKQAYRQKFTFLGKGDWFDVSNIGLNISIPIFSGFA
                     RDSKIKQTKIELQQTENNIGNLKLAINNEVESAKLRFNSAIESMDFQKKNMQLAETVY
                     NQTKKKFEAGTGSNTEINGASARRSMSPPFWSASRVDDT"
     gene            complement(1299..1904)
                     /locus_tag="PLU36_10335"
     CDS             complement(1299..1904)
                     /locus_tag="PLU36_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMZ47191.1"
                     /translation="MQQRIVEEAHKLFLTYGIRSISMDEIALKLGISKKTIYQYYKDK
                     DELVDAVFNIEMHQHKVDCECNIVKCKDAIHEVFLALDSVKETFKAMHPLIFFDLMKY
                     HPGVYKKFNTHKTNFFYEVIKSNIERGVQEGLYRPDINIDIISKYRVVCIFQILENEQ
                     FQEVKVPIVEIMQETTEHFLYGLATPKGARLVKKYTQKRTK"
     gene            2075..>2431
                     /locus_tag="PLU36_10340"
     CDS             2075..>2431
                     /locus_tag="PLU36_10340"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HMZ47192.1"
                     /translation="MKLMLKKYFKILLQFFLQGVVVLAPIGITVWVIVSLFNVIDDLL
                     PNLLSNVFPNYIKKDINGNFEKITGLGFVMVIMLVLFVGWLSSLLQIGKALNIFDKVL
                     ENTPGIKIIYSSVKDFF"
BASE COUNT          690 a          362 c          344 g         1035 t
ORIGIN      
        1 cgtatcgtcc acgcgcgacg cggaccagaa cggcgggctc atcgaccgcc gcgcacttgc
       61 accattaatt tctgtattgc taccagtacc tgcttcaaat ttctttttgg tttggttgta
      121 aactgtttca gctaattgca tatttttttt ctgaaaatcc atactttcaa tagccgaatt
      181 aaaacgtagt ttggcacttt ctacttcgtt atttattgcc aactttaggt tgccaatatt
      241 attttcagtt tgttgtaatt caattttagt ttgtttaatt ttagaatctc ttgcaaagcc
      301 actaaaaata ggaatcgaaa tatttaatcc aatattagat acatcaaacc aatcgccttt
      361 acctaaaaaa gtaaactttt gacgataagc ttgtttgcca tatacaccac taattgcaac
      421 tgtaggtaag tagcttaatt tgtatctttt aatattgtat tcattcaatt tttttactgt
      481 ttgtaaatat tgaaaatctt ttctatcgtt aaaattgata ctatcagtta gagcattttg
      541 cttaatatca ttatctgtta ttttgtctgt tagttctaat gtatcgttta aaggcatacc
      601 aattaatgtt tttaaaccta aatagccaat ttcaatatta ttgagggctt ttactctttc
      661 tgtttttaaa ttagttaatg ttacatcaat tttatctaca tctaattttt ctgcaaagcc
      721 attaatataa agttggttgg catcgtgttt taacttttct attctttcaa tattagtatt
      781 taataattct atttgtgttt tactaactac tagttgatag tatattttgt agatatttac
      841 ttttatattt tccactacta cttcagcatt ttttttagca aattccatag atgcttttct
      901 agcttgtaac ccaacaaata cttgaccatc aaataatagt tgtcttaact gaatcattgc
      961 attggaattg tatttaatac caaattttac aggtacaagt tgtcctggtt gtccaaacat
     1021 atctcctggt ataacttggg tagataccac aatgttattg tttactgtaa tgtctgcacc
     1081 tattgttggg tatgcatttg cagttacttc tctatttacc tgttcttgtg ctaaaatgcc
     1141 taatagtgca ttttttactt gtgcattatt tttacttgcg tactctaccg tttgggtaat
     1201 tgtaaatgaa tgtttggttt gtgcttttat tgtagaatag ttaatgagta ggattgctat
     1261 tagtacaatt tgttttgttt tacttatctg catatattct attttgttct tttttgtgta
     1321 tattttttta ctagtcttgc tccttttggt gttgctaagc catataaaaa atgttctgtt
     1381 gtttcttgca taatttctac tattggcact tttacttctt gaaattgttc attttctaga
     1441 atttgaaata tgcatactac cctgtattta ctgatgatgt caatattaat atctggcctg
     1501 tacaaacctt cttgtactcc tctttcaata ttgcttttaa tcacttcgta aaaaaaattg
     1561 gttttgtggg tattgaattt tttatacaca cccggatggt atttcattaa atcaaaaaat
     1621 attaaagggt gcattgcttt aaaagtttct tttaccgagt ctaatgccaa aaacacttcg
     1681 tgaatggcat ctttacactt aactatatta cattcacaat caactttgtg ctgatgcatt
     1741 tctatattaa aaacagcatc taccaattca tctttgtctt tatagtattg ataaattgtt
     1801 tttttactga tacctaattt taaagctatt tcatccatac ttatacttct aatgccatag
     1861 gttagaaata atttatgggc ttcttctaca attctctgct gcataactaa attattttgt
     1921 gtttttcagg gtgcgaaact atggaaactt tttattgcac aaaagttttt tacgtttttt
     1981 ttgaaaattt tttgtgaatg ggtattagtt tttatgaatg gcaaagtaaa caacatgcat
     2041 atttgtatta cttttaagct cagattatat ttttatgaaa cttatgttaa aaaaatattt
     2101 taaaatttta ttgcaattct ttttgcaggg tgtagttgtt ttagcaccaa ttggtattac
     2161 cgtttgggta attgtttctc tttttaatgt aatagacgat ttgttgccca acttattgag
     2221 taatgttttt cctaattata ttaaaaaaga tatcaatggt aattttgaaa aaattacagg
     2281 attagggttt gtaatggtta ttatgcttgt gctttttgtt ggttggctct cttctttatt
     2341 acaaattggc aaagcattaa atatttttga taaagtttta gaaaatacac caggcattaa
     2401 aattatttat agttccgtta aagatttttt t
//
LOCUS       DAOQPK010000292         2412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_36869_length_2412_cov_3.531608, whole genome shotgun sequence.
ACCESSION   DAOQPK010000292 DAOQPK010000000
VERSION     DAOQPK010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36869_length_2412_cov_3.531608"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PLU36_10345"
     CDS             <1..489
                     /locus_tag="PLU36_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014219891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HMZ47193.1"
                     /translation="ENKKKLPFGLYVVNAVQEEIGLRGAEMIARRIKPNVAIITDVTH
                     DTQTPMINKIIEGDVACGKGPSPTYGPAVHNKLLQLVEDTANKKKIPLQMRTVSRSTG
                     TDTDSFAYANDGCPSVLISIPLRYMHTTVEMLHKKDIENTIQLIYETVVALTPKTNLS
                     YL"
     gene            603..1241
                     /locus_tag="PLU36_10350"
     CDS             603..1241
                     /locus_tag="PLU36_10350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HMZ47194.1"
                     /translation="MIITNSLAEKYAEWLTNNDDELLTQIHQSTSQNHAHAHMLSGHV
                     QGMLLTFMSTLLNPHNILEIGTFTGYSALCLAKGLTVDGQLHTIEIREEDARIAQENF
                     NKSQYSKQLFLHIGNALEIIPTLNKTWDLIFIDADKTGYIKYYEMVLPLLNKNGLIIV
                     DNILFHGQVLEEDIKGKNAIAIDNFNKHIAQDTSVNKVLLTVRDGLLFIKKK"
     gene            1243..2301
                     /locus_tag="PLU36_10355"
     CDS             1243..2301
                     /locus_tag="PLU36_10355"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HMZ47195.1"
                     /translation="MRWTILIALLINFCSYGIAQTEKANNYISLYKEVAINEMRRSGV
                     PAAIILAQGMLESGYGESELSKKSNNHFGIKCKNDWQGEKTYFDDDEKGECFRVYKSV
                     AESYKDHSDFLKSRSWYAFLFKLPPTDYEGWAHGLKKAGYATANDYAPKLIKIITDYN
                     LQQFTLEGLKNSEANTTTKTDKQDSTTIPYHSEDNTETTEEDSTETSTNETRTNISKP
                     LNQDTLKPKHPIYATDSVFTINHSKVIFSAEGTSLLAIANKYNISLSSLFAFNDMKPI
                     ELIDTFRLIFIEKKLKKGAEDNFISNNNETLYDICQLKGVQLESILQYNKGIKKDVKL
                     SKGFIVYLRPQNKIKKGK"
BASE COUNT          946 a          336 c          378 g          752 t
ORIGIN      
        1 gaaaacaaga agaaactacc ttttggttta tatgtagtga atgcagtaca agaagaaatt
       61 ggattgcgtg gtgccgagat gattgcaaga agaataaaac ccaatgtggc aattattact
      121 gatgttacac atgatacgca aaccccaatg ataaataaaa ttattgaagg agatgtagct
      181 tgtggcaaag gcccatctcc tacctatggc cctgcagttc acaataaact tttacaatta
      241 gtagaagaca cagccaataa gaaaaaaatt ccattacaaa tgagaacagt aagtagaagc
      301 actggtacag atacagatag ttttgcttat gccaatgatg gttgcccaag tgttttaatt
      361 tctattcctt tacgttatat gcacactact gtagaaatgc tacataaaaa agatattgaa
      421 aatactattc aactaattta tgaaacagta gtagcattaa cgcctaaaac taatttaagt
      481 tatttatagt tttatacttg attttttttt ctaactgctt ctatagtata ggagcagttt
      541 tttatttaca atttaagagc aattaaaata attttaaaaa caaatgctat tagttttgtg
      601 ctatgattat tacaaattct ttagcagaaa aatatgctga atggcttacc aataatgatg
      661 acgaattgct aacacaaatt catcaatcaa catctcaaaa tcatgctcat gcacacatgc
      721 ttagtggcca tgttcaaggt atgttgttaa catttatgag taccctatta aaccctcata
      781 atattttaga aattggcact tttacaggtt atagtgcttt gtgccttgca aaaggattaa
      841 cagtagatgg ccaactgcat actatagaaa ttagagaaga agatgctaga attgctcagg
      901 aaaactttaa taaatcgcaa tatagcaaac agttattttt gcatataggc aatgctttag
      961 aaattattcc aacattaaac aaaacatggg atttaatttt tattgatgct gataaaacgg
     1021 ggtatattaa atactatgaa atggttttgc ccttattaaa caaaaatgga ttgataattg
     1081 tagataatat attatttcat gggcaagtgc tagaagaaga tattaaaggt aaaaatgcaa
     1141 ttgctataga taattttaac aagcatattg cccaagatac cagtgtaaac aaagttttat
     1201 taacagtaag agatggtttg ttatttatta aaaagaaata gaatgagatg gacaatttta
     1261 atagcactat taattaattt ttgtagttat ggcattgctc aaacagaaaa agcaaataac
     1321 tacattagtt tatataaaga agttgctatt aacgaaatga gaagaagtgg tgtgccagca
     1381 gcaattattt tagcacaagg aatgctagaa agcggatatg gcgaaagtga acttagtaaa
     1441 aaaagcaata accattttgg aataaaatgt aaaaatgact ggcaaggaga aaaaacttat
     1501 tttgatgatg atgaaaaagg cgaatgtttt agagtgtaca aatctgttgc agaaagttat
     1561 aaagatcatt ctgatttttt aaagagccgt tcttggtatg cttttttatt caaattaccg
     1621 ccaaccgatt atgaaggttg ggcacatgga ttaaaaaaag caggatatgc aactgctaat
     1681 gattatgcac ccaaactaat aaaaattatt accgattata atttacaaca attcacttta
     1741 gagggattaa aaaattctga agcaaatact accactaaaa cagataaaca agatagtaca
     1801 actattccat atcattcaga agataataca gaaacaactg aagaagactc tactgaaact
     1861 tctactaatg aaacaagaac aaatatttca aagcctttaa atcaagacac tttaaaacct
     1921 aagcacccaa tttatgcaac cgatagtgtt tttaccatca atcactctaa agtaattttt
     1981 tctgccgaag gcacttcttt gctagcaatt gccaataaat acaatatttc tctaagttct
     2041 ttatttgcat ttaacgatat gaaaccaata gagttaattg atacttttag attaattttt
     2101 attgagaaaa aattaaaaaa aggtgcagaa gataatttta tttccaataa caacgaaacc
     2161 ctctatgata tttgtcagtt aaaaggtgta caacttgaaa gtattctgca atacaataaa
     2221 ggaattaaaa aagatgtaaa attaagtaaa gggtttattg tatatcttag accacaaaat
     2281 aaaattaaga aagggaaata acccattatt ctattatcct taaaatcata aatagttatg
     2341 caaaatagta tttcacttca taataaagta tttgttcctt ttatttcaga gcaagaaatt
     2401 gctgataaaa tt
//
LOCUS       DAOQPK010000293         2395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_37345_length_2395_cov_4.148291, whole genome shotgun sequence.
ACCESSION   DAOQPK010000293 DAOQPK010000000
VERSION     DAOQPK010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37345_length_2395_cov_4.148291"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="PLU36_10360"
     CDS             <1..1179
                     /locus_tag="PLU36_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HMZ47196.1"
                     /translation="GIDWYINQLRYKVNESAPIDVIWSADQIEGRKRDYVPFKPNPKF
                     PESAYYDLYDVMKNYIGSDNPAFIDNSRGEPLNTFPVKKFAIPVNKDEIIKNGIVNAT
                     DSILAELRFDLNKDRLLKNDLAVLNIIAANKWKRPICFTSNNPYQIGLGFERFLRMDG
                     LTYRLVPVDNSNGTFNTNAPLVNTDWAYDKMMNNFAAGNANVKGVYFDEENRRHLNSI
                     REAYTELAFDLISKNRMNDAKKVLQHCDSLMLQENVPYGMVSRANQHNRNSLRFLTAC
                     YEVGDKDLAAKVLKSLKTDIEQQQVYYASLGDGKTSVEAYKKLMDDYITRYNSARTRQ
                     DQVTADNILQSNLSQRQINSLNDIRMSYMLLVELNNVEITNNKTQQVPAQTSVDTTKK
                     "
     gene            complement(1244..1741)
                     /locus_tag="PLU36_10365"
     CDS             complement(1244..1741)
                     /locus_tag="PLU36_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HMZ47197.1"
                     /translation="MAKTVKKEQTTNESIVLDNKDFAILKLLQQNAKATVKDIAKQIH
                     LSTTPVHERIKRLEHLGVIKQYATLLDYAKVKKSLMVFCYVSLKEHSKNAGSKFIKHI
                     HSLPEVIECFNISGEFDFMLKVVAESMDDYYHFHVNKLSQSENIANVQSVFVMGVLKQ
                     THIVI"
     gene            1861..>2395
                     /locus_tag="PLU36_10370"
     CDS             1861..>2395
                     /locus_tag="PLU36_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HMZ47198.1"
                     /translation="MSTLTKTIDFTLPYKVADISLAEWGRKEIRLAEAEMPGLMSIRA
                     EYGASQPLKGARIAGCLHMTIQTAVLIETLVALGAEVKWSSCNIFSTQDHAAAAIAAA
                     GIGVYAWKGQTQEEADWCIEQTLFFGGADRPLNMILDDGGDLTNMVFDKYPEFVSGIK
                     GLSEETTTGVHRLYERMV"
BASE COUNT          869 a          318 c          411 g          797 t
ORIGIN      
        1 ggtatagatt ggtacattaa tcagttgcgt tataaagtaa acgaaagtgc accaattgat
       61 gtgatttgga gtgcagacca aattgaaggt aggaaaagag attatgtacc atttaaacca
      121 aacccgaaat ttccagaaag tgcttattac gatttgtatg atgtaatgaa aaattatata
      181 ggtagtgata atcctgcatt tatagataac tcaagaggtg agcctttaaa tactttccca
      241 gttaaaaaat ttgctattcc tgttaataaa gatgagataa ttaaaaatgg cattgtaaat
      301 gctacagatt ctattttagc agaacttaga tttgatttaa ataaagacag attattgaaa
      361 aacgatttgg ctgtattaaa tattattgct gccaataaat ggaaacgtcc tatatgtttt
      421 acaagcaata acccatacca aataggctta ggttttgaaa gatttttaag gatggatgga
      481 ttaacttaca gattggtgcc tgtagataac agcaatggaa catttaatac caatgctcct
      541 ttggtaaata ccgattgggc atacgataaa atgatgaata attttgctgc aggaaatgca
      601 aatgtaaaag gagtatattt tgatgaagaa aatagaaggc atttaaactc tataagagaa
      661 gcctatacag aattagcatt tgatttaata agtaaaaaca gaatgaatga tgctaaaaaa
      721 gtattacaac attgcgattc attaatgcta caagagaatg ttccttatgg aatggtaagt
      781 agagcaaatc agcacaatcg taatagttta aggtttttaa ctgcatgcta tgaagtagga
      841 gataaagatt tagctgcaaa agtattaaaa agcctaaaaa cagatattga gcaacaacaa
      901 gtttactatg caagtttagg tgatggtaaa acatctgttg aggcttataa aaagctaatg
      961 gatgattata ttactcgata taattctgct cgtacaaggc aagatcaggt tactgctgat
     1021 aatattttac aaagcaattt atctcaacgt caaattaatt cattaaacga tattagaatg
     1081 agctatatgc tgcttgttga attaaataat gtagaaatta ctaacaataa aacacaacaa
     1141 gttccagcac aaacaagtgt ggatacaaca aaaaaataat aaataaaact agtgtaaaat
     1201 aaggttatgt aaaatttcaa ttaaatttta tgtagcctta tttttatatt acaatatggg
     1261 tttgttttaa tacacccatt acaaaaacac tctgcacatt tgcaatattt tcactctggc
     1321 taagtttgtt aacatgaaaa tgataataat catccatgct ttctgctact acttttaaca
     1381 taaagtcaaa ctcgccagaa atattaaaac actcaattac ttctggtaaa ctatgaatat
     1441 gtttgataaa ttttgagcct gcatttttgc tatgttcttt taaacttaca taacaaaaaa
     1501 ccattaaact ttttttcact tttgcataat ctaataaagt ggcatattgt tttattactc
     1561 ccaaatgttc aagcctttta attctttcat gtacaggcgt tgtgcttaaa tgaatttgct
     1621 tggcaatgtc ctttacggtt gcttttgcat tttgttgaag tagttttaaa atggcaaagt
     1681 ctttattatc taatacaatg ctttcattag tggtttgttc tttttttact gttttagcca
     1741 taagttactt attatttgtt ctaaatatta ctataaaatt aataaaatat tctaaattat
     1801 taatagtttg caaaaaatta ttctttatta aatacttttg cgttctaata aaaataaaat
     1861 atgtcaacgc ttacaaaaac aattgatttt acattgccat acaaagtggc agatattagt
     1921 cttgcagagt gggggagaaa agaaattcgt ttagccgaag cagaaatgcc aggtttaatg
     1981 agtattcgtg ctgagtacgg agcatctcaa ccattaaaag gtgcaagaat tgcaggttgt
     2041 ttacacatga ccattcaaac tgcagtttta attgaaacat tagttgcttt aggtgcagaa
     2101 gttaaatgga gttcttgtaa cattttttct acacaagatc atgctgctgc tgctattgct
     2161 gctgctggca ttggagtgta tgcttggaaa ggtcaaactc aagaagaagc tgattggtgt
     2221 atagaacaaa cattattttt tggtggtgca gatagaccat taaatatgat attagatgat
     2281 ggaggcgatt taacaaatat ggtatttgat aaataccctg aatttgtttc aggcataaaa
     2341 ggtttgagtg aagaaactac aacaggtgtg catcgtttat atgaaagaat ggtaa
//
LOCUS       DAOQPK010000294         2386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_37609_length_2386_cov_3.341484, whole genome shotgun sequence.
ACCESSION   DAOQPK010000294 DAOQPK010000000
VERSION     DAOQPK010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37609_length_2386_cov_3.341484"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="PLU36_10375"
     CDS             <1..891
                     /locus_tag="PLU36_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HMZ47199.1"
                     /translation="KKIALFVKEGNIVILESTSPVGTTEKVAEVLSNNGVNINNVYVA
                     YCPERVLPGKIMKELVENDRIVGGINTQSTNKVVAFYKTFVQGEVLATNARTAEMCKL
                     TENSYRDVNIAFANELSMICDKADINVWELINLANHHPRVKILQPGAGVGGHCIAVDP
                     WFIVSDFPTEAKLIKAGREVNDYKTEWVVEKIKNTALQFELNNKHKPVIACMGLAFKP
                     DIDDLRESPALEIAKCINDEVCTVVCVEPNVNELKGLTLVSLNQAIEQADIICYLVKH
                     SAFKGLKNTTNKIELDFCDV"
     gene            946..2241
                     /locus_tag="PLU36_10380"
     CDS             946..2241
                     /locus_tag="PLU36_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="HMZ47200.1"
                     /translation="MKLSTLIDSKNINWPLYFYLLILTQSIFFIKFFAFPLFFLFHFT
                     DFKIVRKYGIQYKFYIAIITYAALFCLLYLLKDFNLQYLIVVLLGLFVWLLCLINNFI
                     VNYSVHKLQLKKLYATIQFYFLLNIVLSFFQFLLLIAENKTINPFSTALGTSAGDFIK
                     GYFANSSINMIVSSFAFVFFYARKNYLLAILSLMVLILTTYMSGVLIFIVIFVLYFLL
                     TSTTSLIQKIQLLILGFIFSISFYLLSKDNIDYAVRVGSTVVEEVKPRKQVSFEQTGE
                     AILSSTSVFFFGYGMGNFSSRLAFIAGGEYVSWYPTALVRKSVEFYNNHFQLWNYEVL
                     DVPFSDGTANQPFSVFNQFLGEYGFIGTIILLYFYFYSLFKQSKKYSFIRLLLVLLFF
                     YMLLDYWFEFFSVIVVFELIVAYWLKEASEKIKHKQIVA"
BASE COUNT          800 a          294 c          381 g          911 t
ORIGIN      
        1 aaaaaaatag cactctttgt taaagaaggt aatattgtaa ttttagaatc aacttctcct
       61 gtgggcacaa cagaaaaagt agcagaagtt ttaagcaata atggggttaa tatcaataat
      121 gtttatgtag catattgccc tgaacgtgta ttgcctggaa aaataatgaa agaattagta
      181 gagaatgata gaattgttgg tggcattaat acacaaagta ccaataaagt agttgctttt
      241 tataaaacat ttgtacaagg cgaagtgtta gcaaccaatg ccagaactgc agaaatgtgt
      301 aaacttacag aaaactctta ccgagatgta aacattgcat ttgccaatga actaagtatg
      361 atttgtgata aagcagatat aaatgtttgg gagttaatta atttagcaaa ccatcatcca
      421 agagtaaaaa ttttacaacc aggtgcaggt gttggtgggc attgtattgc tgttgaccct
      481 tggtttattg tatctgattt tcctacagaa gccaaattaa taaaagctgg tagagaagta
      541 aatgattata aaacagaatg ggttgtagaa aaaataaaaa acacagcttt acagtttgaa
      601 ttaaataata aacacaaacc agttatagct tgcatgggat tggcgtttaa gccagatatt
      661 gatgatttaa gagaaagccc tgcattagaa attgccaaat gtattaatga tgaagtttgt
      721 actgttgtat gtgttgagcc aaatgtaaat gaactaaagg gcttaacatt agtttcacta
      781 aaccaagcaa tagagcaagc ggatattatt tgttatttag taaaacacag tgcatttaag
      841 gggttaaaaa ataccaccaa taaaattgag ttagattttt gtgatgttta atttaggtat
      901 ttaattatca acaattaaac tactgcaaat aagaaaagta atttaatgaa actttctact
      961 ttaatagata gtaaaaacat caattggccg ttatactttt acttattaat acttactcaa
     1021 agtatttttt ttataaaatt ttttgcattt ccactttttt ttctttttca ttttaccgat
     1081 tttaaaattg taagaaaata tggaatacag tataaatttt atattgcaat tattacttat
     1141 gcagctttgt tttgcttgtt atatttatta aaagatttta acctgcagta ccttattgta
     1201 gtattgttag gcttgtttgt ttggttgtta tgtttaataa ataattttat tgtaaactat
     1261 agtgtacaca agctacaact taaaaaattg tatgctacca tacaatttta ttttctcctt
     1321 aatattgttt tatcattttt tcaattttta ttattaatag cagaaaataa aactatcaac
     1381 cctttttcta cagctttagg taccagtgca ggagatttta ttaaaggcta ttttgccaac
     1441 tcttctatca atatgatagt ttcctctttt gcatttgttt ttttttatgc tagaaaaaac
     1501 tacttactgg ctatactttc cctaatggta ttaatactta caacgtatat gtctggtgtg
     1561 cttattttta ttgtcatatt tgttctatat tttttactta cttctacaac aagcctaatt
     1621 caaaaaattc aattattaat attaggattt attttctcca tttcatttta tttactctca
     1681 aaagataata tagattatgc agtaagagtt ggaagtacag ttgtagaaga agtgaaacct
     1741 agaaaacaag tgtcttttga gcaaacaggc gaagccatct tatcttcaac ttcagttttc
     1801 ttttttggat atggaatggg aaatttttca tcgagattag catttattgc tggtggagaa
     1861 tatgtatctt ggtatcccac agcattggta agaaaatctg tagagtttta taataatcac
     1921 tttcagctat ggaattatga agttttagat gttccatttt ccgatggtac tgctaatcag
     1981 ccattttctg tttttaatca atttttaggt gagtatgggt ttattggaac aatcatttta
     2041 ttatattttt atttctatag tttatttaag caatcgaaaa agtatagttt tattaggctg
     2101 cttttagtat tgctgttttt ttatatgcta ttagattatt ggtttgaatt tttctctgtt
     2161 attgttgttt ttgaattaat agttgcttac tggctaaagg aggcttcaga aaaaattaag
     2221 cataaacaaa ttgttgctta attattatta ttttgaagaa agagataatc ataaatactt
     2281 ttcatttatt ttttaacaaa ggggttaatg ctcttgttcc tttgctttta ataccattat
     2341 taaacgaaaa atttggaata gttggctatg gacagttagt gtatat
//
LOCUS       DAOQPK010000295         2384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_37649_length_2384_cov_4.923572, whole genome shotgun sequence.
ACCESSION   DAOQPK010000295 DAOQPK010000000
VERSION     DAOQPK010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37649_length_2384_cov_4.923572"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(741..1004)
                     /locus_tag="PLU36_10385"
     CDS             complement(741..1004)
                     /locus_tag="PLU36_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47201.1"
                     /translation="MMEKIELLHGRATPKIDRATLLHKKKVLMNEKNVLLHDGATPEI
                     DRATLLHNKIVLMNEKNVLLHSGATPKTDGAALLHEKIVHMER"
     gene            1432..2130
                     /locus_tag="PLU36_10390"
     CDS             1432..2130
                     /locus_tag="PLU36_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47202.1"
                     /translation="MNSTISYIPNSDSERNVWLNNFSTKISTYANLVGISAAEVTAIQ
                     KDTAMFNYIINMLEAYKQTVNNITSYKNLLKHAVGQQHLGAIPSPPTLGTAPATVPEG
                     VFDRISKLVKRIKASANYTEAMGNDLGIIAPTQTIDITAMQPDLKVSLDADRPHIKCS
                     KGYADAIDLYVDRKDGAGFVLIGRLLKLDYIDVVSLPTNTVLAEWDYKARYVIGNDPV
                     GLMSAVVSVVVKKS"
BASE COUNT          782 a          387 c          397 g          818 t
ORIGIN      
        1 ttattaaata tttattgccg tctttatcaa aaatagtatc aaatacacta cccggagtta
       61 aaggatccag gccaggtact tgagctctta aatctgatat actaatagta taaatcagaa
      121 aaaataatag tgttagtttt tgtttcataa ttggttgatt ttgttttaca agattaattt
      181 atttttttaa ttgagtatat gatttttgtt aactgtatct tatttttaaa taagtgtgcg
      241 gcttttttgt atagatgatt atatgcttat agccataaat tgtatatcat taaactgtac
      301 ttaaattcac agtcgtattt gttatttttc ataaaacata taattaaact aaagttcaat
      361 aagctaacat aattttatat tcattacaaa aatatagtgg cggcacaaca aactatcaca
      421 agtttacgca cctgatattt tagtaaaatc aatacaatat taaattgatg ataaaactat
      481 gttagttttt atacagtatc aataggtatt tgtacctgtt tttgtgttgt tacttacagg
      541 tataaatacc tgtcattatt ataagcaata aacataatat aattagccat aaaaagcatc
      601 cgtatttata ctgatttttt ctacgtattt atacgtacgt ataaatacgg atatataaac
      661 taaaattgta gtttttgcaa taagtaaagt aactacataa cattaatatt acttttacca
      721 tttacacaga taagtggaca ttacctctcc atatgtacta ttttctcatg gagcagagct
      781 gctccgtcag ttttgggagt tgctccactg tggagcagaa catttttttc attcatgagt
      841 actattttat tgtggagcag agttgctctg tcaatttcgg gagttgctcc gtcgtggagc
      901 agaacatttt tttcattcat gagtactttt tttttgtgga gcagagttgc tctgtcaatt
      961 ttgggagttg ctctgccgtg gagcagctca attttttcca tcatatgtac tattttttaa
     1021 cggagcagaa ctgctccgtt attattggga gctgctcttt taaaaagcaa ttgttaaaac
     1081 cggctttctg attttgctct gcaataaata atgcttgtgt ttttggtacc acttacaaaa
     1141 taaaatctac cacttattgc caggttacag ctaacggtta aaaaactggc gtttttacat
     1201 tttttaagac actgttaaaa ttatttttgg cacaagcatt gaaaacacta gtatcaacaa
     1261 gctttttttt aaaccataaa ataaaaatat aatatgccac caaaaattaa attataatga
     1321 gtataaaaaa accaaccagt gttttattat actacaagtg ctaaagccat acttgttgac
     1381 tcatttggct ttaaaaataa attaaagtca aatttttaaa ttattattat tatgaactca
     1441 acaataagtt atattcctaa ctccgatagt gagaggaatg tatggttgaa taacttttca
     1501 accaaaatta gtacctatgc caacctggtt ggtatatcag cagccgaggt aactgccata
     1561 caaaaagata ccgccatgtt taattacatt attaatatgc tggaagcgta taagcaaacg
     1621 gtaaataata ttacctctta caaaaatcta ttaaaacacg ctgtggggca acaacactta
     1681 ggagctatcc ctagcccccc aactttaggt accgctccgg caacagtgcc cgaaggagtg
     1741 tttgatagaa ttagtaaatt ggtaaaacgc atcaaagcta gtgctaacta taccgaggca
     1801 atgggtaacg acttaggtat tattgctcca actcaaacca tcgatattac tgccatgcaa
     1861 cccgatttaa aagtaagttt ggatgccgac agacctcaca ttaaatgcag taaaggttat
     1921 gctgatgcga ttgaccttta tgtagacaga aaagatggtg caggttttgt gttaattggt
     1981 cgcttactta aactcgatta cattgatgta gtgagtttac ctaccaacac cgtacttgcc
     2041 gaatgggact acaaagcacg ttatgtaatt ggtaatgatc ctgtaggttt aatgagtgct
     2101 gtggtaagtg tagtagttaa gaagtcgtag caaacatagc ttgtcacatc gagtgattcg
     2161 acgaaggaat cccgatagct atcgggagta tcgagatgtg agtttagact accgttctct
     2221 atataatttt gttgcaagag gcaagcaaca aaatcactcg aactgacagt ttttaaaaac
     2281 aacaataata aaccccctct gttagcaata gcagaggggg ttttattttt attttactct
     2341 cgataataat gtaacatgtc cggttttaat tactttttct cccg
//
LOCUS       DAOQPK010000296         2377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_37866_length_2377_cov_4.381568, whole genome shotgun sequence.
ACCESSION   DAOQPK010000296 DAOQPK010000000
VERSION     DAOQPK010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37866_length_2377_cov_4.381568"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="PLU36_10395"
     CDS             <1..826
                     /locus_tag="PLU36_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ47203.1"
                     /translation="IGLYHQPPFYREMRRYDGTINNNLKAQKSLQFSAGMDYNFTMFN
                     RPARITTEAYYKNMWQVVPYDIDNVRIRYTGENNAKAYAIGLETRLYGELVKDAESWL
                     SFGIMQTKENLTNDAYQKYTLDNNNIPIDSTLINVGWLRRPTDRLITFGMFFQDYLST
                     NKNFKFYMNALYGSNLPYNIPGSVRYRNALRIEPYIRVDIGISALLLQPEKQNRRSYS
                     PFKNFESIWVSLEAFNLIDRANTISYLLIKDYQNNTFAMPNRLTPRLINLKLIAKW"
     gene            complement(828..1979)
                     /locus_tag="PLU36_10400"
     CDS             complement(828..1979)
                     /locus_tag="PLU36_10400"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HMZ47204.1"
                     /translation="MKLQTSRIESIDILRGIVMIIMALDHTRDFYSNILFDPLDLTKT
                     NVPLFFTRWVTHFCAPIFVFLAGTSAYLYLSKGKTKKQAATFLFTRGLWLIFVELTII
                     NFAWSFDYTFSFVGVQVIWAIGWSMIFLSLLVYLKPLYIGIIGILIIATHNSLDNIHV
                     ESFGNYKYLWAFLHEQYFFPYNNNSRFIGIMYPIIPWIGVIAAGFWFGTLYKLEFAKR
                     KNILLLLGIACLLLFVLIRYSNQYGDLNIWTKQSNWYFTILSFINCTKYPPSLLFLLI
                     TLGIAFIVLAFLENVQSKILNVVKVYGKVPFFYYILHILFIHASAKLVAIVMGIQATN
                     VFDNVNNQWGFGLTGVYVAWAVNVALLYIPCLWFMKVKQKRKDWWLSYL"
     gene            complement(2078..>2377)
                     /locus_tag="PLU36_10405"
     CDS             complement(2078..>2377)
                     /locus_tag="PLU36_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HMZ47205.1"
                     /translation="RHIERNSCLLFVIPADSNNHKNEFEILVNELKEYNPELLQKDFL
                     IAVSKTDMLDEELKNEISKELPNNIPHIFISSIMQQGLTELKDALWKILNKPATL"
BASE COUNT          913 a          341 c          326 g          797 t
ORIGIN      
        1 aataggctta taccatcaac caccatttta cagagaaatg agaagatacg atggtactat
       61 aaataataat ttaaaagcac aaaaaagcct acaatttagt gcaggcatgg attataattt
      121 taccatgttt aacaggcctg caagaattac tacagaagct tactataaaa atatgtggca
      181 agtggtgcct tatgatattg ataatgtaag aatacgttat acaggagaaa ataatgctaa
      241 agcttatgcc attggtttag aaaccagatt atatggcgaa ttggttaaag atgcagaaag
      301 ctggttaagc tttggtatta tgcaaacaaa agaaaactta actaatgatg cttatcaaaa
      361 atatacttta gataataaca atataccaat tgatagtact ttaataaatg taggttggtt
      421 aagaaggcct acagataggc taattacttt tggaatgttt tttcaagatt atttaagtac
      481 taacaaaaat tttaaatttt acatgaatgc cttgtatggc agcaatttgc cctataatat
      541 acctggtagt gtacggtata gaaatgcttt aagaatagaa ccttatataa gagtagatat
      601 tggtattagt gcattattat tacaaccaga aaaacagaat agaagaagtt atagcccttt
      661 taaaaatttt gaaagtattt gggtaagttt agaagcattt aatttaattg atagggctaa
      721 taccattagt tatttattaa taaaagatta ccagaataat acttttgcta tgcctaacag
      781 gcttacccca cgattaataa acttaaagct aattgctaaa tggtagttta taaataactc
      841 aaccaccaat cttttctttt ttgttttacc ttcataaacc ataagcatgg tatgtatagt
      901 aaagcaacat taactgccca agctacataa acaccagtta gcccaaagcc ccattggtta
      961 tttacattat caaaaacatt tgttgcttgt atgcccatta caatggctac taattttgcc
     1021 gaagcatgta tgaataaaat atgcagtata tagtaaaaga aaggcacttt gccatatacc
     1081 ttaactacat ttaaaatttt agattgtaca ttttctaaaa atgctaatac aataaatgca
     1141 atacccaaag taattaataa aaataaaaga gatggaggat atttagtaca gttgataaaa
     1201 gataaaatgg taaaatacca gttgctttgt tttgtccaaa tatttaaatc tccatattgg
     1261 ttgctgtatc ttattaaaac gaataatagc aaacatgcaa ttcctaataa taataaaata
     1321 ttttttcttt tggcaaattc taatttatat aaagtaccaa accaaaagcc tgcagcaata
     1381 acaccaatcc acggaattat tggatacatt attccaataa atctggaatt attattatag
     1441 ggaaagaaat attgttcatg caaaaaagcc cataggtatt tataatttcc aaagctttct
     1501 acatgaatat tgtctaaact attgtgtgtt gcaataatta aaataccaat tatgccaata
     1561 tacaatggct ttaaataaac caataaagat aaaaaaatca tactccaacc aattgcccaa
     1621 ataacttgta caccaacaaa agaaaaagtg taatcaaaac tccaggcaaa attgatgatt
     1681 gttaattcta caaaaattaa ccacaaacct cttgtaaata aaaaagttgc tgcttgtttt
     1741 tttgttttgc ctttacttaa atataaataa gcacttgtac cagctaaaaa aacaaaaatg
     1801 ggtgcacaaa aatgagttac ccaacgagta aaaaataatg gaacatttgt tttagttaaa
     1861 tctaaagggt caaataaaat attgctataa aaatctcttg tatggtctaa tgccattatt
     1921 atcattacaa taccacgtaa aatatcaata gattctattc tacttgtttg cagtttcatt
     1981 ggttttagtt ttaaagcaat tactaaaagt aagtactctt caataaaaat gtaaaatttt
     2041 tatttaaact aaaattagca atggtttgta attacaatta aagcgtagca ggtttattta
     2101 atattttcca tagtgcatct tttaattcag ttaacccctg ttgcattata gatgaaataa
     2161 aaatatgtgg aatattgtta ggcagttctt tactaatttc attttttagt tcttcatcta
     2221 acatatctgt tttgcttaca gcaattaaaa aatctttttg taataactcg gggttatact
     2281 cttttaattc atttactaat atttcaaatt catttttatg gttattgcta tcggcgggta
     2341 ttacaaaaag caaacaactg tttctttcaa tatgcct
//
LOCUS       DAOQPK010000297         2376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_37894_length_2376_cov_5.246445, whole genome shotgun sequence.
ACCESSION   DAOQPK010000297 DAOQPK010000000
VERSION     DAOQPK010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37894_length_2376_cov_5.246445"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PLU36_10410"
     CDS             <1..263
                     /locus_tag="PLU36_10410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HMZ47206.1"
                     /translation="FNNIVVDKKDYNIKLIAHCNLKEKVPLQKISIKENTMLLIGPEG
                     DFTEDEIFLAEKNNFIAVSLGNNRFRTETAGIAAAVLLKLNN"
     gene            266..1441
                     /locus_tag="PLU36_10415"
     CDS             266..1441
                     /locus_tag="PLU36_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47207.1"
                     /translation="MQIKEVNTKSLENDFITVNVLMNKSNKAYIRPLDNDINDIFNPN
                     KNKAYQNGNAKRWVLVDENGNLIGRIAAFISSKYINKNTNYLTGGIGFFDCINNQNAA
                     NTLFNTAKDWLQQNGAEAMDGPINFGDRDKWWGLMVEGFNDEPVFGMPFNPAYYQNLF
                     EQYGFKNYYNQYWYKMMVHDPLPARFKERYEKFAAKPDYYAKHADLKNLEKYAADFAK
                     VYNTAWAQHGEGKEVTKEQILKLFNTMKPIMDVRVIWFAYFKDEPIAMFINIPDINQY
                     IKYFNGKLNLLNKLKLLWMKKNRKHKRLTGLAFGVIPKFQALGVDSYIIQSCANLIQN
                     KNWYESYEMGWAGDWNPRMVNIYKSLGGSQNRRMVTYRYIFDESKYSFERHPVMEYN"
     gene            1488..1874
                     /gene="apaG"
                     /locus_tag="PLU36_10420"
     CDS             1488..1874
                     /gene="apaG"
                     /locus_tag="PLU36_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HMZ47208.1"
                     /translation="MISKISEGIKVNVECNYNTKHSKPILGDFIFSYCITIENYNAFT
                     VQLLKRKWHIFDSLGAYREVEGDGVVGVQPIIKPGDHFQYVSFCNLKTEIGKMQGSYE
                     LLNLNTLETFYVTIPEFELIAPLKYN"
     gene            complement(1871..>2376)
                     /locus_tag="PLU36_10425"
     CDS             complement(1871..>2376)
                     /locus_tag="PLU36_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HMZ47209.1"
                     /translation="RGKTKVGNNCLIMAYTHVAHDCIIGNNCILSNSVQMAGHVVVGD
                     YAIIGGVSAVHQFVHIGQHTFIAGGSLVSKDVPPFMKAVRNPLSYGGVNSVGLKRRGF
                     TLEQINHILDIYRIIYNKGLNTSQAIDYIEQEIQASDERDDIIMFIRESGRGIIKRFT
                     KGANDED"
BASE COUNT          890 a          326 c          378 g          782 t
ORIGIN      
        1 gttttaataa tattgtagtg gataaaaaag attataatat taagcttatt gcccattgta
       61 accttaaaga gaaagtaccc ttacaaaaaa tttcaattaa agaaaataca atgcttttaa
      121 ttggccccga aggagatttt acagaagatg aaattttttt agccgaaaaa aataatttta
      181 tcgctgtatc attaggtaat aacaggttta gaacagaaac tgcaggtata gcagcagcag
      241 tattattaaa attaaataat taaaaatgca aataaaagaa gtaaatacaa aatctttaga
      301 aaatgatttt attactgtaa atgtgttaat gaacaaaagt aataaggcat atattaggcc
      361 tttagataat gatattaacg atatttttaa ccctaacaaa aacaaagcat atcaaaacgg
      421 caatgctaaa agatgggttt tagtagatga aaatggaaat ttaattggaa gaattgcagc
      481 ttttatttct tcaaaatata tcaataaaaa taccaattac ttaacaggcg gtataggttt
      541 ttttgattgt ataaataatc aaaatgctgc caatacttta tttaatacag caaaagattg
      601 gcttcagcaa aatggtgcag aggctatgga tggaccaatt aattttggag acagagataa
      661 atggtggggg ttaatggttg aaggttttaa tgatgagcct gtttttggaa tgccttttaa
      721 ccctgcttac taccaaaatt tatttgagca atatggcttt aaaaattatt ataaccaata
      781 ttggtataaa atgatggtac atgacccttt accagcaaga tttaaagaaa gatatgaaaa
      841 atttgctgct aagccagact attatgcaaa acatgccgat ttaaagaatt tagaaaaata
      901 tgctgccgat tttgcaaaag tttataatac tgcatgggca caacatggcg aaggaaaaga
      961 agtaaccaaa gagcaaattt taaagctttt taatactatg aaaccaatta tggatgttag
     1021 ggtaatttgg tttgcatatt ttaaagatga accaattgct atgtttataa acataccaga
     1081 cattaaccaa tatattaagt actttaatgg taagttaaat ttacttaata aacttaaatt
     1141 gctttggatg aaaaaaaaca gaaaacataa aaggcttaca gggcttgcat ttggtgtaat
     1201 tcctaaattt caagcattag gggtggatag ttatatcata caatcttgtg ctaatttaat
     1261 tcaaaacaaa aactggtatg aaagttatga aatgggttgg gcaggagatt ggaatcctag
     1321 aatggtaaat atttataaaa gcttaggtgg tagccaaaat agaagaatgg ttacctatcg
     1381 ttatattttt gatgaaagta aatattcgtt tgagaggcat cctgtaatgg agtataatta
     1441 acaagattta aaaagtttta aacttatctt tacttaaaca ataatcaatg atttcaaaaa
     1501 tttctgaagg cataaaagtt aatgtagagt gtaattataa cactaaacat agcaagccta
     1561 ttttgggaga ttttatcttt tcttattgta taacaattga aaattataat gcttttaccg
     1621 ttcaactact aaaaagaaaa tggcatattt ttgatagcct tggagcttac agagaggtag
     1681 aaggtgatgg tgttgtgggt gtacaaccta ttattaaacc aggagaccat tttcaatatg
     1741 ttagcttttg caatttaaaa acagaaattg gcaaaatgca aggcagttac gagttattaa
     1801 atttaaatac tttggaaact ttttatgtta ctattccaga atttgagttg attgcaccat
     1861 taaaatataa ctaatcttcg tcgttggcac ctttagtaaa acgttttata attcctctac
     1921 cactttctct aataaacatt ataatatcat ctctttcatc actagcttga atttcttgtt
     1981 caatatagtc aatagcttgc gaagtgttta accctttatt atatattatt ctataaatat
     2041 ctaaaatatg atttatttgc tctaaagtaa agcctctgcg ttttaagcct acactattta
     2101 caccaccata gcttaatgga tttctaacag ctttcataaa aggaggtacg tctttactaa
     2161 ctaaactacc acctgcaata aaagtatgtt ggccaatgtg cacaaattga tgtacagcac
     2221 ttacacctcc aataatagca taatctccta ctacaacatg cccagccatt tgtacactat
     2281 tacttaaaat acaattattg ccaataatac aatcatgtgc cacatgggtg taagccataa
     2341 ttaagcagtt attgccaact tttgttttac ctctat
//
LOCUS       DAOQPK010000298         2348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_38746_length_2348_cov_3.279546, whole genome shotgun sequence.
ACCESSION   DAOQPK010000298 DAOQPK010000000
VERSION     DAOQPK010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38746_length_2348_cov_3.279546"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PLU36_10430"
     CDS             complement(<1..587)
                     /locus_tag="PLU36_10430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HMZ47210.1"
                     /translation="MKKKTALIIGFIGLKFLFHFFLISNEYDLQRDEFLHLDQANHLA
                     WGYLSVPPFTSWTSYIIQLFGNSFFWVKFFPALYGALTIFIVWKSIEELNGNIFALTL
                     GATCVLFSALLRINILYQPNSLDILCWTAFYFILLKYIHTENFKWLYIGAVVFALGFL
                     NKYNIIFLLIGLLPALLLTTHRKIFFKPNLYFAALL"
     gene            complement(629..1516)
                     /locus_tag="PLU36_10435"
     CDS             complement(629..1516)
                     /locus_tag="PLU36_10435"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HMZ47211.1"
                     /translation="MKSGIRFVMFFFTLTTLSVQAQRVRLSGALGGSAYYGDLIQGTP
                     LLKQISPAVTLGGSYDLNEKLRVRASLSGLSVKADDKDNSNIKFKNRNLNFKSFIWEF
                     AVAAEYDILNNFDNYAYTPYIFIGPGIFGFNPTTVDRFGNKVNLREWGTEGQGLAAYP
                     NRKPYSRTQFNIGFGGGIRVDLSESLQIGTEIFIRKTFTDYIDDVSINSYVDPSLFVL
                     QGNPYSQYVAFRGDEVGKSFSTTMPRGNPKNKDLFYSFQLKLTYKLLNLDWGGPMGYY
                     GGGSPGRTRDRVRTPRSVL"
     gene            complement(1699..>2348)
                     /locus_tag="PLU36_10440"
     CDS             complement(1699..>2348)
                     /locus_tag="PLU36_10440"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HMZ47212.1"
                     /translation="NHKPIQYLTNDAWFCNKKYYVNEHVLIPRSETEELIYLLKEDEH
                     SFNNIIDIGTGSGCIAIEVKNKFKNSTVVAIDKSLDSLEIAKKNALTFNTKINFKHLD
                     FLEENNWADLGNFDLIISNPPYVLKSEQNTLKENVLNFEPHLALFVPDNDPFLFFRKI
                     ALFGKKHLNKHGKIVVEINELFGYETKQLFIEFGYLSYIIKDMQQKDRFIVSNFS"
BASE COUNT          875 a          368 c          287 g          818 t
ORIGIN      
        1 agtaaggctg caaaatatag attgggttta aagaaaattt ttctatgtgt tgttagcaac
       61 aaagcaggca atagaccaat taataaaaaa ataatattgt atttattcaa aaaacctaat
      121 gcaaaaacta cagcacctat atacaaccat ttaaaatttt ctgtatgtat gtattttaaa
      181 agtataaagt aaaaagctgt ccaacataat atatctaaag agttgggttg atacagtatg
      241 tttattcgta atagtgctga aaataacaca caagttgcac ctaatgtcaa agcaaaaata
      301 tttccattta attcttctat agatttccat acaataaaaa ttgttaaagc accatataaa
      361 gctgggaaga atttaaccca aaaaaatgaa ttgccaaata attgaattat gtacgatgtc
      421 caagaagtaa agggtggaac agaaagatat ccccaagcta aatggtttgc ttggtcaaga
      481 tgcaaaaatt catctcgttg caagtcatac tcattgctaa ttaaaaaaaa atgaaacaaa
      541 aattttagcc caatgaaacc aattattaat gccgtctttt ttttcataaa gggctataag
      601 taaaaccaat aattacaaaa aataaatatt atagtacaga acgaggagtt cttactctat
      661 ctcttgttct tccagggctt ccaccaccat aataacccat tgggccaccc caatctaagt
      721 tcaataattt ataagttaat tttaattgaa aggagtagaa caaatctttg tttttagggt
      781 tgccacgagg cattgtagta gaaaaagatt ttcctacttc atctcctcta aacgctacat
      841 actgagagta tgggttgcct tgtaaaacaa ataaagaagg gtctacataa ctgttaatac
      901 ttacatcatc aatataatcg gtaaaagttt tacgaataaa tatttcggta ccaatttgta
      961 aactttcact taaatcaact cttataccac caccaaaacc aatattaaat tgtgtacgag
     1021 aataaggttt tctattagga taagcagcta aaccttgccc ttctgtaccc cattctctta
     1081 aatttacttt attcccaaat ctatctactg ttgtagggtt aaatccaaaa ataccagggc
     1141 caataaaaat atatggtgta taagcatagt tatcaaaatt gtttaaaata tcatattcgg
     1201 ctgcaactgc aaattcccat ataaaactct taaagtttaa atttctattt ttaaacttaa
     1261 tattactatt gtctttatca tctgctttca cacttaaacc agataagctt gctctaactc
     1321 ttaatttttc atttaaatca taactacctc caagagttac tgcaggtgaa atttgtttta
     1381 ataatggcgt tccttgaatt aaatctccat aataagcact accccctaat gcaccagata
     1441 atcttactct ttgagcttgc acagataagg tagttaaagt aaagaaaaac atcacaaaac
     1501 gtattccaga tttcatactc ataaaattta acttttcagt tattgccaaa attaatgcaa
     1561 aatattaaac aaaatatttt ttatgctaca ataataggct tttcattaca aaatattaat
     1621 tttcaataat tttttttaca aaagtatata aaaagggtgt tattttttgt tgaaaaatgc
     1681 tttataatta ttgaattatt aagaaaaatt acttacaata aacctatctt tttgttgcat
     1741 atctttaata atataggata gataaccaaa ctctataaat agctgctttg tttcataacc
     1801 aaataattca tttatttcta caacaatttt tccatgttta ttcaagtgtt tttttccaaa
     1861 caaagcaatt tttctaaaaa acaagaaagg gtcattgtcg ggtacaaata aagccaaatg
     1921 tggttcaaag ttcagtacat tttcttttaa tgtattctgc tcacttttta aaacataagg
     1981 tgggttacta attattaaat caaaatttcc taaatctgcc cagttatttt cttctaaaaa
     2041 atctagatgt ttaaaattta tttttgtatt aaaagttaaa gcatttttct tagcaatttc
     2101 taaactatct aaacttttat ctattgctac aacagtagag tttttaaatt tattttttac
     2161 ttcaattgca atacaaccag aaccagtacc aatatcaata atattattaa atgagtgctc
     2221 atcttctttt agtaaataaa taagttcttc tgtttcagaa cgaggtatta aaacatgttc
     2281 atttacataa tattttttat tgcaaaacca tgcatcattg gttaggtatt gaatgggttt
     2341 atgattta
//
LOCUS       DAOQPK010000299         2336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_39111_length_2336_cov_4.373959, whole genome shotgun sequence.
ACCESSION   DAOQPK010000299 DAOQPK010000000
VERSION     DAOQPK010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39111_length_2336_cov_4.373959"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="PLU36_10445"
     CDS             <1..1197
                     /locus_tag="PLU36_10445"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HMZ47213.1"
                     /translation="ALAVLATNFYNNPSKKINLIGVTGTNGKTTIATLLFKLFTQLGY
                     KCGLISTVQNQIENKISPSTHTTPDAIKLNELLKQMVEENCTHVFMEVSSHAIHQHRI
                     TGLEFKGGIFSNITHDHLDYHKTFDEYIKVKKKFFDDLPSNAFAISNADDKRGAVMLQ
                     NTKASKHFYSLKSIEEFKGKILDNALTGLQMLVNDKEVHFRLIGEFNAYNLLAVYATA
                     ICLNENKEKVLTALSMLTGAEGRFDYIIGNQLIIGIVDYAHTPDALENVLTTINKLRK
                     GHEQIITVVGCGGDRDKTKRPLMAQTACDLSDKVILTSDNPRTESPEQILKDMEVGLN
                     TAAKRKYITITDRREAIATAVNMAKGEDIILVAGKGHEKYQEINQIKYPFDDKEILNN
                     LLNKQQ"
     gene            1212..>2336
                     /locus_tag="PLU36_10450"
     CDS             1212..>2336
                     /locus_tag="PLU36_10450"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HMZ47214.1"
                     /translation="MLYHLFTWLKNEFNLPGSGLFQFLTFRIAMAVLLSLLIATVYGK
                     RIIIYLQKKQIGESVRDLGLEGQMQKKGTPTMGGIIILLSILIPTLLFANLDKVYIRL
                     MILCTVWLGIIGFLDDLLKIRARKAAQQKGEAYKKKDSDGLAGISKIIGQIGLGIIIG
                     ATLYFSNAVTVEREVIGTTYQKQAEEKFAKDSTIFSRNIDGVEKRYVTVKTPITTIPF
                     VKNHEFNYSKLISWIGSGAEKYTWIVYIFIVTLIITAVSNGANLTDGLDGLAAGVSAI
                     IGIGIGLFVYVSGNYKFADYLNIMYIPNLGELSIFVAAFVGACIGFLWYNAYPAQVFM
                     GDTGSLALGGIIASLAIIVRKELLIPIFCGIFLVENLSVII"
BASE COUNT          858 a          310 c          432 g          736 t
ORIGIN      
        1 gcattagctg tattagctac aaatttttat aacaaccctt ctaaaaaaat taatttaatt
       61 ggcgttacag gcaccaatgg aaaaacaact attgcaacac tattatttaa attatttaca
      121 cagttaggat acaaatgtgg gttaataagt actgtgcaaa atcaaataga aaataaaatt
      181 tctccttcca ctcatacaac accagatgca ataaaattaa atgagttgct gaaacaaatg
      241 gtagaggaaa attgtactca tgtgtttatg gaagtaagca gccatgcaat acatcaacat
      301 agaattacag gcctagaatt taaagggggc atttttagca acataaccca tgatcattta
      361 gattatcaca aaacctttga tgaatatatt aaagtaaaga aaaaattctt tgatgattta
      421 ccgtcaaatg cttttgcaat aagtaatgca gatgataaac gtggtgctgt tatgcttcaa
      481 aacaccaaag cttctaaaca tttttatagc ttaaagtcaa tagaagagtt taaaggaaaa
      541 attttagata atgcacttac tggtttgcaa atgctggtaa atgacaagga agttcatttt
      601 agattaatag gagaatttaa tgcttataat ttattagcag tgtatgcaac agcaatttgt
      661 ttaaatgaaa acaaagaaaa agtattaact gctttaagca tgttaacggg tgccgagggt
      721 agatttgatt atattatagg caaccaatta attattggaa ttgtggatta tgctcataca
      781 ccagatgctc tagaaaatgt attaactaca ataaataaat taaggaaagg gcatgagcaa
      841 attattactg tggtaggctg tggtggagat agagataaaa ctaagaggcc tttaatggca
      901 caaactgcct gcgatttaag cgataaagta attcttacca gcgataaccc aagaacagaa
      961 tctcctgaac aaattttaaa agatatggaa gtaggattaa acactgctgc caaaagaaaa
     1021 tatataacaa taaccgatag aagagaagcc attgctactg ctgtaaatat ggcaaaaggc
     1081 gaagatatta ttttggttgc aggcaaaggg catgaaaaat atcaggagat aaaccaaata
     1141 aaataccctt ttgatgataa agaaatatta aacaatttat tgaataaaca acaatagtag
     1201 aaaatcaatt tatgctatac catttattta catggctaaa aaacgaattt aacttaccag
     1261 gctctggctt gtttcagttt cttacgtttc gtattgcaat ggcagtgctt ttatcgttgc
     1321 ttatagctac tgtatatggt aaacgaatta ttatttactt acaaaaaaag caaattggag
     1381 agagtgtgag agaccttggc cttgaaggtc aaatgcaaaa aaaaggtacg ccaacaatgg
     1441 gtggaataat tattttatta agcattttaa ttccaacatt gttatttgca aacttagata
     1501 aagtgtatat acgattaatg attttatgca ctgtgtggct tggtattatt ggctttttag
     1561 acgatttatt aaaaataaga gcaagaaaag ctgctcagca aaaaggcgaa gcttataaaa
     1621 agaaagacag tgatggccta gcaggtataa gtaaaattat tggtcaaatt ggtttaggta
     1681 ttattattgg tgccacatta tattttagta atgcagtaac tgtagaacgt gaagtaatag
     1741 gcacaaccta tcaaaaacaa gcagaagaaa aatttgcaaa agactccact atattttcta
     1801 gaaatattga tggtgtagag aaaagatatg ttactgttaa aacaccaatc acaactatac
     1861 cttttgttaa aaatcatgaa tttaattaca gtaaattaat tagctggata ggaagtggtg
     1921 ctgaaaaata tacttggata gtgtacattt ttattgttac actcatcata acagctgtta
     1981 gtaatggtgc aaatttaaca gatggtttag atggtttggc tgcaggtgta agtgcaatca
     2041 ttggtattgg tattggcttg tttgtatatg taagcggtaa ttacaagttt gccgattatt
     2101 tgaatattat gtatataccc aatttgggtg aactaagcat ttttgttgca gcatttgttg
     2161 gtgcatgtat cggatttttg tggtacaatg cttatcctgc acaagttttc atgggcgata
     2221 ctggtagttt ggctttaggt ggcatcattg cttcattagc cattattgta agaaaagaat
     2281 tattaattcc aatattctgc ggaatatttc ttgttgaaaa tttaagcgta atcatt
//
LOCUS       DAOQPK010000300         2319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_39650_length_2319_cov_4.332155, whole genome shotgun sequence.
ACCESSION   DAOQPK010000300 DAOQPK010000000
VERSION     DAOQPK010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39650_length_2319_cov_4.332155"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="PLU36_10455"
     CDS             complement(<1..1007)
                     /locus_tag="PLU36_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ47215.1"
                     /translation="MKFVFHILFIVFVFTSPTIFGQKTATITGKIIDEEHKPIVGASI
                     AILGKNVGTISNETGFFSLKITSNKVISVVFSFTGYVPVQKNMVLNTGETDFITIQLK
                     KDTATLLEVEVKDDRKKREPGSIHIDPSKAGVNPSPIQGIEQLIKIFVGSNNELTSQY
                     SVRGGSYDENLIYVNDFEVFRPYLIRNGQQEGLSFINPELTSGIKFYNGGFASKYGDK
                     MSSVLDITYKKPKQFGGSVYIGLLEQGLHIEGNNKKNNLSYLLGVRNRSNRNLLSSQE
                     TKGNYIPSSSDVQALINYRINNKWQVELFGNLSASKFTLYPEESKLTSSIFSPFYTAS
                     FG"
     gene            1135..2298
                     /locus_tag="PLU36_10460"
     CDS             1135..2298
                     /locus_tag="PLU36_10460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMZ47216.1"
                     /translation="MKRIFTLSLLFTFFSTSLLAQEVIARWNFEVLDFSSATGNTFTV
                     GAGSVVADSGALVTGSEFSANHAAATSAWTTPAGNGSANSASSSGWASGDYWQFKAST
                     TGFNSITIVYDQMGSNTGPKSFLVQYSTDGVTFTDVPSSGYDIANDAWSASTYKPVST
                     RNLSLAAITALNNQPAIYIRLTVAAGSTAINGSAIATGGTSRIDNFYIGGIALAPLTL
                     NSFTASAVNGKANLNWFTTNEVNVDGFDIEKSNNGKDFTSVGFVKANNKTENAYSFTN
                     ELNGISYYRLKMIDKDGTFKYSKTVSLNGKTTVKLDLYPNPVRNTTTLSHDKATDRAT
                     IKITTLGGKTVNVVYVQNGATQTTIDVSKLLNGNYIMVYENNGTRSSVQFVKQ"
BASE COUNT          777 a          366 c          363 g          813 t
ORIGIN      
        1 ccaaaactag cagtataaaa aggagaaaat atggaactgg ttaatttact ttcttcagga
       61 tataaagtaa actttgaagc agataaattg ccaaataact caacctgcca tttattgttt
      121 atgcgataat taattaatgc ttgcacatcg gacgaggagg gtatataatt tccttttgtt
      181 tcctgactgc ttagtaaatt tctattgctt ctgtttctta cacccaataa ataacttaaa
      241 ttattttttt tattgttacc ttctatatgc aaaccttgtt ctaataaacc aatataaacc
      301 gagccaccaa actgctttgg ttttttataa gtaatatcta atacactact cattttatcg
      361 ccatatttag aggcaaagcc accattgtaa aatttaatgc cacttgttaa ttcaggatta
      421 ataaaactta acccctcttg ttgcccattt cttattaaat aaggtctaaa cacttcaaaa
      481 tcgtttacat aaattaaatt ttcatcgtaa ctgccaccac gtacagagta ttgagaagtt
      541 aattcgttat tactacctac aaaaatttta attaattgtt caattccttg aatgggcgaa
      601 gggttaacac ctgctttaga tggatctata tgtatgctgc ctggttctct tttttttcta
      661 tcatctttta cttctacctc taaaagtgtt gcagtatctt tttttaattg aatagtaata
      721 aaatcggttt cgccggtatt taataccatg tttttttgta ctggcacata acctgtaaaa
      781 gaaaacacta cacttattac tttattggaa gtaattttta agctgaaaaa accagtttcg
      841 ttactaatag ttcctacatt ttttccaaga attgcaatac ttgcaccaac aataggttta
      901 tgctcttcat ctataatttt accagttatg gtagctgttt tttggccaaa aattgtagga
      961 gaagtaaata caaaaactat aaataatata tggaaaacaa acttcattaa gtacttttaa
     1021 agcaatgaag ataagtattg taccatttta atattaacct aagattaatt tttaataaaa
     1081 atatatgttg aattaatttt tattgcttat tttgtggcaa ctaaaataaa ttttatgaaa
     1141 agaatattta cgctttcgtt gttattcact tttttttcta caagcctgct tgcacaagag
     1201 gtaattgcaa gatggaactt tgaggtatta gatttttctt ctgcaacagg taataccttt
     1261 actgttggtg ctggttctgt agttgcagat tctggtgctc tagtaacagg ctcagaattt
     1321 tctgccaacc atgctgctgc tacttctgcc tggactactc cagcgggtaa tggttctgct
     1381 aattctgcaa gtagtagtgg ctgggcatct ggcgattatt ggcaattcaa agcatctacc
     1441 acaggtttta attctattac aatagtttat gaccaaatgg ggagcaacac gggtcctaaa
     1501 tcttttttag tacaatacag tacagatggt gtaacgttta ctgatgtacc aagttctggt
     1561 tatgatattg caaatgatgc atggtctgca agtacctata aaccagtttc tactcgtaat
     1621 ttaagtttag ctgcaattac agcgttgaat aaccagcctg ctatttatat tagattaaca
     1681 gttgctgcag gtagcacagc aattaatgga agtgctattg caacaggagg cacttctaga
     1741 attgataatt tttatattgg tggtatagca cttgcaccac ttactttaaa ttcttttact
     1801 gcatcggcag ttaatggtaa agctaatctt aattggttta ccactaatga ggtaaatgta
     1861 gatggttttg atattgaaaa aagcaataat ggtaaagatt ttacaagtgt tggctttgta
     1921 aaagcaaata acaaaacaga aaacgcttat agctttacta acgagttaaa tggaatttct
     1981 tattatcgtt taaaaatgat agataaagat ggtacattta aatacagcaa aacagtaagc
     2041 ttaaatggta aaacaactgt taagttagat ttatatccta accctgttag aaatactaca
     2101 actttatctc atgataaagc tacagataga gcaactatta aaattactac tttaggaggc
     2161 aaaacagtta atgtagtgta cgtacaaaat ggtgcaacac aaactacaat agatgtaagt
     2221 aaattattaa atggtaatta tattatggtg tatgaaaata acggcacaag aagctctgta
     2281 caatttgtaa aacaataatt atattttcac taatattca
//
LOCUS       DAOQPK010000301         2304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_40118_length_2304_cov_2.845265, whole genome shotgun sequence.
ACCESSION   DAOQPK010000301 DAOQPK010000000
VERSION     DAOQPK010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40118_length_2304_cov_2.845265"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /gene="proS"
                     /locus_tag="PLU36_10465"
     CDS             <1..1184
                     /gene="proS"
                     /locus_tag="PLU36_10465"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HMZ47217.1"
                     /translation="DPNNKGKLMVDPEAKLEEELVVRPTSEAIIWNTYKGWIQSYRDL
                     PILVNQWANVVRWEMRTRLFLRTAEFLWQEGHTAHSTKEEAIIETEKMLDVYADFVEN
                     WMALPVIKGIKTANERFAGAEETYCIEALMQDGKALQAGTSHFLGQNFAKAFDVKFSD
                     KNNKLEYVWATSWGVSTRLIGGLVMAHSDDDGLILPPKIAPIQVVIVPIYKTENEKAL
                     IDEKVNTLVNQLKVNNIRVKYDDNDNARPGWKFAEYEMKGVPIRLAIGARDLANNVVE
                     IARRDTKEKQSVSMDELNIYIQNLLATIQENMFNKAKNFRDEHITNVNTWDEFITTLD
                     NTTGFVNAHWDGTPETEEKIKELSKATIRCIPLNNKEEAGNCILTGKPSKQRVLFARA
                     Y"
     gene            complement(1240..1695)
                     /locus_tag="PLU36_10470"
     CDS             complement(1240..1695)
                     /locus_tag="PLU36_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HMZ47218.1"
                     /translation="MKSYQGKIICNTVLMNDENFIDTTIFITEHNQNGAIGFIINKPF
                     YKSLNQLEEFKNCLAVPLYFGGPVDNEHLFFIHQRNDLIKGGTLITNNFYFGGDFKQA
                     ITLLNQQLITTSDIKIFIGYCGWNMYELEEEINEGCWTVDTYNINLVFQ"
     gene            1927..>2304
                     /locus_tag="PLU36_10475"
     CDS             1927..>2304
                     /locus_tag="PLU36_10475"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HMZ47219.1"
                     /translation="MDRKVTNIGVLTSGGDSPGMNAAIRAVTRTGLYHGLGVYGIMRG
                     YAGMIEGDIKKLDGISVANIIQRGGTFLKTARSKEFHTYEGRKKAYNNLKQLNIDGLV
                     IIGGDGSFNGAQIFSNEFDIPCIG"
BASE COUNT          845 a          288 c          419 g          752 t
ORIGIN      
        1 cagaccctaa taataaaggg aaattaatgg tagatcctga agccaaatta gaggaagaat
       61 tagtcgttcg ccctaccagt gaagctatta tttggaatac ttacaaagga tggatacaat
      121 cttacagaga tttaccaatt ttagtaaatc aatgggcaaa tgtggttcgt tgggaaatgc
      181 gtacaagatt atttttacgc actgctgagt ttctttggca agaaggacat acagcacatt
      241 ctactaaaga agaggccatt attgaaactg aaaaaatgtt agatgtatat gccgattttg
      301 tagaaaactg gatggcttta cctgtaatta aaggaattaa aacggcgaat gaaagatttg
      361 ccggagcaga agaaacctat tgtattgagg ctttgatgca agatggaaaa gcattacaag
      421 ctggaacatc tcatttctta ggtcaaaatt ttgctaaagc atttgatgtt aaatttagcg
      481 ataagaacaa taagcttgaa tatgtttggg ctacaagttg gggtgttagt accagattaa
      541 ttggtggttt agtaatggca cacagcgatg atgatggttt gattttacct cctaaaattg
      601 ctcctataca agttgtaatt gtacctattt ataaaacgga aaatgaaaaa gcattgattg
      661 atgaaaaagt aaacacttta gtaaatcaat taaaagttaa taatattaga gttaaatatg
      721 atgataacga taatgcaaga ccaggctgga aatttgcaga gtacgaaatg aagggtgtgc
      781 ctattcgttt ggctattggt gcaagagatt tagctaataa tgttgttgaa attgccagaa
      841 gagatacgaa agaaaagcaa agcgtaagta tggatgaatt gaatatttat attcaaaact
      901 tacttgcaac aattcaggaa aatatgttta ataaggctaa aaattttaga gatgaacata
      961 ttaccaacgt aaatacttgg gatgaattta ttacaacatt agacaataca acaggctttg
     1021 taaatgcaca ttgggatggc acacctgaaa cagaagaaaa aattaaagaa cttagcaaag
     1081 ctactattcg ttgtattcct ttaaacaata aggaagaagc aggaaattgt attttaacag
     1141 gaaaaccaag caaacaaagg gttttatttg ctagggctta ttaatttata attttatttt
     1201 atagtaaaaa gctacaataa gtttattgta gcttttgttt tattggaata ccagatttat
     1261 attgtaagta tctactgtcc aacaaccttc atttatttcc tcttctaatt catacatatt
     1321 ccaaccgcag tatccaataa aaatttttat atcgctggtt gtaataagtt gttggtttaa
     1381 taaggtaata gcttgtttaa agtctccacc aaaataaaag ttgtttgtaa ttaaagttcc
     1441 tcctttaatc aagtcatttc tttgatgtat aaaaaataaa tgttcgttat ctacaggtcc
     1501 tccaaaatat aaaggtacag caaggcaatt tttaaattct tctaattggt tcagtgattt
     1561 ataaaatggt ttattaatga taaaacctat agcaccattt tggttatgtt ctgtaatgaa
     1621 aatggttgta tctataaaat tttcatcatt cattaaaact gtattacaaa taatttttcc
     1681 ttggtaagat ttcattcttg taaagttatt gcaaagttta taaaattttg ttgattttga
     1741 agtacaaact aatagtccaa taaaatttta aaatattaaa aaaatagctt ttaaccaact
     1801 tacttgaaag agttacaaac ttcatttttt tgaattgttt atataataga gtaatattgc
     1861 cggctgctaa caaaagaaca atttgcaata taattacctt tactagttac tgtaaataag
     1921 tttgaaatgg atagaaaagt taccaatata ggagtgctaa catctggtgg agatagccca
     1981 ggaatgaatg ctgccataag agctgttacc agaacaggtt tataccatgg tttaggagtt
     2041 tatggtataa tgagaggata tgcaggtatg atagaaggcg atataaaaaa attagatgga
     2101 attagtgttg ccaacattat tcaaagagga ggaacttttt taaaaactgc tcgtagtaaa
     2161 gagtttcata cctatgaagg cagaaaaaaa gcatacaata atttaaagca attaaatatt
     2221 gatggtttag taattatagg aggagatggt agttttaatg gtgctcaaat tttttctaac
     2281 gagtttgata ttccttgtat tggc
//
LOCUS       DAOQPK010000302         2296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_40347_length_2296_cov_4.427934, whole genome shotgun sequence.
ACCESSION   DAOQPK010000302 DAOQPK010000000
VERSION     DAOQPK010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40347_length_2296_cov_4.427934"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PLU36_10480"
     CDS             complement(<1..320)
                     /locus_tag="PLU36_10480"
                     /inference="COORDINATES: protein motif:HMM:NF025167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HMZ47220.1"
                     /translation="MGNFQLYFDIGFKHIVNIGALDHILFMAAIALRYQFFDFKKLLI
                     IVTAFTIGHAITLALAILQLISLPKNWIEFLIAVTIIVTSFSNLFVKKFVYKGKFPPI
                     YYFA"
     gene            432..920
                     /locus_tag="PLU36_10485"
     CDS             432..920
                     /locus_tag="PLU36_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47221.1"
                     /translation="MVNSLFNWLLVGCIALLHPFYVSKVEINHNAKDKNVEISIRIFT
                     DDLELTLRKFGNTPINLNAPKDIVATDKLINKYIQDKLRITIDDKISSIQYLGFEINK
                     ESVWIYYEIPQINTIKKVGINCSLLYDYKQEQMNIIQLTANGKDVSYKLDNPKSYVSF
                     SF"
     gene            complement(940..>2296)
                     /gene="accC"
                     /locus_tag="PLU36_10490"
     CDS             complement(940..>2296)
                     /gene="accC"
                     /locus_tag="PLU36_10490"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HMZ47222.1"
                     /translation="LASLHMFKKILIANRGEIALRVIRTCREMGIKTVAVYSTADKES
                     LHVRFADEAVCIGKPAGADSYLNIQHIMAAAEITNADAIHPGYGFLAENAKFSQICAD
                     HSIKFIGPTPEMINKMGDKITAKETMIAAGVPVVPGSGGLLQSVEQAKDLAKNKVGYP
                     IILKATAGGGGKGMRVVWKEEELEKAYDTAKAEAAASFKNDGIYMEKFVEEPRHIEIQ
                     VAGDQYGNFCHMSERDCSIQRRHQKLVEESPSPFMTPELRKAMGEAAIKAAAAINYES
                     VGTIEFLVDKHRNFYFMEMNTRIQVEHCVTEEVINFDLIKEQIKIAAGEKISGKNYEP
                     GMHAIECRINAEDPYNDFRPSPGKITTLHTPGGHGVRVDSHVYAGYTIPPYYDSMIGK
                     LITIARTREEAIDTMYRALSEYVIEGIKTTIPFHLQLMQNEDFRNGNFTTKFLETFKL
                     K"
BASE COUNT          741 a          378 c          344 g          833 t
ORIGIN      
        1 aatgcaaaat aatagatggg cggaaacttg cctttgtaaa caaatttttt tacaaataaa
       61 ttactgaatg atgttacaat tattgtaacg gctattaaaa actcaatcca gtttttaggt
      121 aaactaatta attgaagaat ggctaatgct aaggttattg catggcctat tgtaaaagct
      181 gttacaataa ttagcagttt tttaaaatcg aaaaattggt atctaagtgc aatggcagcc
      241 ataaacaaaa tatggtctaa tgctccaata tttacaatat gtttaaatcc aatatcaaag
      301 tataactgaa aatttcccat agatttaagt cagtaattat ttgattaaaa ttaaaaaatt
      361 aagccaacta tgttcttttc taaacttgaa atttaagtag gtttattacc ttagcaaaat
      421 aatgatttta aatggtaaat agtttattta attggttatt ggttggttgt attgctttat
      481 tacatccttt ttatgtaagt aaagtagaaa ttaatcataa tgcaaaagat aaaaatgtag
      541 aaatatctat aagaatattt acagatgatt tggaacttac tttacgaaaa tttggcaata
      601 ctcctattaa ccttaatgcc ccaaaagata tcgttgcaac tgataaactt atcaataaat
      661 atattcaaga taaattaaga attaccatag atgataaaat atcttctata caatatttag
      721 gctttgaaat aaataaggaa agtgtatgga tttattatga aatacctcaa ataaatacca
      781 taaaaaaagt tggcattaac tgttctcttt tatatgatta taagcaagaa caaatgaata
      841 ttattcagct aacagcaaat ggtaaagatg taagctataa gttagataat cctaaaagct
      901 atgttagttt tagtttttag tttaatattt cttataaaat tattttagtt taaatgtttc
      961 taaaaatttt gtggtaaaat ttccatttct aaaatcttcg ttttgcataa gttgtaaatg
     1021 aaaaggaatg gttgttttaa ttccctcaat tacatactca ctaagtgcac ggtacatagt
     1081 atctatagct tcttcccttg ttctggcaat agttattaat ttaccaatca tactatcgta
     1141 ataaggagga attgtatagc ctgcataaac gtggctatct acacgaactc catgcccacc
     1201 aggtgtatgt aaagtagtaa tttttcctgg cgatggtcta aagtcgttat aagggtcttc
     1261 tgcatttatt ctacattcaa ttgcatgcat tccgggttca taattttttc ctgaaatttt
     1321 ttcgcctgct gcaattttta tttgctcttt aattaaatcg aaattaatta cttcttcggt
     1381 tacgcaatgc tctacctgaa tacgagtatt catttccata aagtaaaagt tgcgatgttt
     1441 atctactaaa aactcaatgg tacctacgct ttcataattt attgcagctg cagctttaat
     1501 agcagcttct cccattgctt tacgaagttc tggggtcata aaaggcgatg gtgattcttc
     1561 tactagtttt tgatggcgac gttgaatact acaatctctt tcgctcatgt ggcaaaaatt
     1621 gccatattgg tctcctgcaa cttgtatttc tatatggcgt ggttcttcta caaatttctc
     1681 catatagatt ccatcgtttt taaacgatgc agcagcttct gcttttgcag tatcgtaagc
     1741 cttctctagt tcttcctctt tccaaacaac tctcattcct ttaccaccac caccagcagt
     1801 tgcttttaaa ataatggggt aacctacttt attttttgct aaatcttttg cttgttcaac
     1861 actttgcaat agaccgccac taccaggtac aacaggtacg cctgcagcaa tcatggtttc
     1921 tttggctgtt attttatcac ccattttatt aatcatttcg ggtgtagggc caataaattt
     1981 tatactatga tcggcacata tttgagagaa ttttgcattt tctgctaaaa aaccatagcc
     2041 tggatgaatt gcatcggcat tggtaatttc tgcagcagcc attatatgct gaatgtttaa
     2101 ataactatcg gcacctgctg gttttcctat acatactgct tcgtcggcaa aacgtacatg
     2161 taaactttcc ttatctgctg ttgaataaac tgcaacggtt ttaattccca tttctctaca
     2221 agtacgtatt acacgtaatg caatttcgcc tctattggca attaatattt ttttaaacat
     2281 atggagtgat gctaat
//
LOCUS       DAOQPK010000303         2288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_40602_length_2288_cov_3.732199, whole genome shotgun sequence.
ACCESSION   DAOQPK010000303 DAOQPK010000000
VERSION     DAOQPK010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40602_length_2288_cov_3.732199"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(255..917)
                     /gene="plsY"
                     /locus_tag="PLU36_10495"
     CDS             complement(255..917)
                     /gene="plsY"
                     /locus_tag="PLU36_10495"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HMZ47223.1"
                     /translation="MNEFLFIILAYLIGSIPTSIWVSKRFFNIDIREYGSGNAGATNT
                     YRVLGPKWGTFVMIIDMLKGVVATSLYIFLPSYLGISNEWDRTNLMIGLGLAAVVGHI
                     FPIWANFKGGKGVATLFGMAVAIQPLVAVCCIAVFLFVLYLTRFVSLSSILAGVAFMV
                     FILFIFNEETTLYRVFAVMVALMVILTHQKNIGRIIKGTEGKVPILKFRDNRKKRNRK
                     PF"
     gene            complement(999..1811)
                     /gene="prmA"
                     /locus_tag="PLU36_10500"
     CDS             complement(999..1811)
                     /gene="prmA"
                     /locus_tag="PLU36_10500"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HMZ47224.1"
                     /translation="MNYIQLKIEVSNEAMQELVIALLADNGYNGFETTLTNVLAYIDE
                     IEYSESLVKKILQPMQIKFIATVIEKQNWNEIWETNFPSVLVDNFVGIRANFHQALHN
                     VQHEIIITPKMSFGTGHHETTFSVIQLMQEIDFNNKTVFDFGTGTGILAILAEKLGAK
                     KVVAVDNDEWCIENSIENVNANNCKNIIVQNAENAITNQKYDIVIANINKNIILQNIQ
                     ALHNAVKNNGFILLSGLLKDDEDEIKMVTSQIGWQPIKTIAKNNWIALCYTS"
     gene            complement(1871..>2288)
                     /locus_tag="PLU36_10505"
     CDS             complement(1871..>2288)
                     /locus_tag="PLU36_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HMZ47225.1"
                     /translation="IQEIQKVTGTTINIEEVGNTGEVSIFSQEKESLLKAVEWVKGIV
                     AVPQVGEVYDATVKSIKEFGAFVEFLPKKEGLLHISEISWKRLESMDGIFKEGDKVKV
                     KLLEIDQRTGKFKLSRKALMPRPERQDKPQQPTVEG"
BASE COUNT          767 a          415 c          272 g          834 t
ORIGIN      
        1 gaccgtggtg gaaatggcgg tggttataac agatattaat ccttaatatt aacacacact
       61 ttatagaaaa aatcgtttca attttgaaac gattttttta ttttatagat aaacccttat
      121 ctaatttgaa tattaagttt tatgtttaat gcacctcaca aaactatttt ttaattgaat
      181 agaaattaca atggtattga tgcaaataaa gaaattgaac aaaaactaca gcaaagcatt
      241 tcaggagtat aaatttaaaa tggtttccta tttctttttt ttctattgtc tctgaatttt
      301 aaaataggca ctttcccttc cgttccttta ataattcttc ctatattttt ttgatgggta
      361 agaataacca ttaatgcaac cattactgca aaaaccctat ataatgtagt ttcttcatta
      421 aaaataaata aaataaaaac cataaaagct acaccagcca aaatagaact taatgaaaca
      481 aaccgagtaa gatacaaaac aaataaaaat acagcaatac aacaaacagc aaccaaaggt
      541 tgtatggcaa ctgccattcc aaataaagtt gcaacacctt tacccccttt aaaatttgcc
      601 catataggaa atatatgccc aaccacagca gccaagccta aaccaatcat taaattggtt
      661 ctatcccact cattgcttat tcctaaataa gatggcaaga aaatatataa ggaagtagct
      721 accacccctt tcagcatatc aataatcatt acaaaagtac cccatttagg tcctagaact
      781 ctataggtat tagttgctcc agcattacca cttccatatt ctctaatatc aatattaaag
      841 aaccttttgc ttacccaaat agaagtaggt atagagccaa ttaaatatgc caaaataata
      901 aacaaaaact cgttcattgc gttggttgaa ggttggaaaa ttcaaatata caatctgttt
      961 gtaaataaaa atgcacaatt tattaaaaca acaagttatt aacttgtata acacaaagct
     1021 atccaattgt ttttggctat agttttaata ggttgccagc caatttgaga tgtaaccatt
     1081 tttatttcat cttcatcatc cttcaataat ccacttaata aaatgaagcc attatttttt
     1141 acagcattat gtaatgcctg aatattttga agaataatat ttttatttat gtttgctatt
     1201 acaatatcat atttctgatt agtaattgca ttttctgcat tttgaacaat gatattttta
     1261 cagttgtttg cgtttacatt ctcaatactg ttttcaatac accactcatc attatctact
     1321 gcaacaactt tttttgcacc taatttttct gcaagaattg ctaatatacc agtgcctgtg
     1381 ccaaaatcga aaactgtttt attattaaaa tcaatctcct gcattaattg aattacagaa
     1441 aatgtagttt catgatgtcc tgttccaaaa ctcattttag gagtgataat tatttcatgt
     1501 tgtacattat gtaaagcttg gtgaaaattt gctctaatac ctacaaaatt atctaccaat
     1561 acagacggaa aatttgtttc ccatatttcg ttccaatttt gtttttcaat aactgttgca
     1621 atgaatttta tttgcattgg ttggagaatt tttttaacca aactttcgct gtattctatt
     1681 tcatcaatat atgctaacac attggttaat gttgtctcaa aaccattgta cccattatct
     1741 gcaagcaatg caattactaa ctcttgcatg gcttcattgg aaacctctat ttttaattgt
     1801 atataattca ttttaaaaaa taatcccccg aatttttgta ctcgggggaa tttaatttag
     1861 attaacaact ttagccttca acagtaggtt gttgtggttt atcttgcctt tctggtcttg
     1921 gcattaatgc ttttctgctt agtttaaatt taccagtacg ctggtcaatt tctaataatt
     1981 tcacttttac tttatctcct tctttaaata ttccatccat actttctaat cgtttccaac
     2041 tgatttcgct aatatgtaat aagccctctt tcttaggtaa aaattctaca aacgcaccaa
     2101 actctttaat actttttaca gtggcatcat aaacctctcc tacttgaggt actgctacaa
     2161 tacctttaac ccattctact gctttaagta aactttcttt ttcttgagag aagatgctta
     2221 cctcaccagt attacctact tcttctatat taatagttgt acctgttact ttttgaattt
     2281 cttgaatt
//
LOCUS       DAOQPK010000304         2257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_41603_length_2257_cov_3.727066, whole genome shotgun sequence.
ACCESSION   DAOQPK010000304 DAOQPK010000000
VERSION     DAOQPK010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41603_length_2257_cov_3.727066"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PLU36_10510"
     CDS             complement(<1..271)
                     /locus_tag="PLU36_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMZ47226.1"
                     /translation="MSVLIKNGRIITADSDQTADIFISGETVQAIGKNLNVQSDKEID
                     ATGLLIFPGGIDPHVHLDMPFMGTFSSDSYETGTRAALYGGTTMVL"
     gene            complement(327..704)
                     /locus_tag="PLU36_10515"
     CDS             complement(327..704)
                     /locus_tag="PLU36_10515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HMZ47227.1"
                     /translation="MTENEISNKVIGNGIEVHSALGPGLLESAYKECLYYKLISSGFL
                     VEKEKAMPLVFENVKLECGYRIDLLVEKKLVIEIKSVEALNDIHLAQTLTYMKLGNYK
                     LGLLMNFNVLRLKDGIKRVVNGL"
     gene            complement(727..2154)
                     /locus_tag="PLU36_10520"
     CDS             complement(727..2154)
                     /locus_tag="PLU36_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HMZ47228.1"
                     /translation="MNNSLYNEDLAPIPQDKRTWGTWNYAALWISMSLCIPTYMLASG
                     MIKEGLNWWQAIVIFLLGNSIVLIPMILNGHAGAKYGIPFPVFARASFGTRGANIPAI
                     LRAIVACGWFGIQTWIGGESIYNLIRTWNPTLEEIHVTGLFPQALPFICFLAFWFLNM
                     LIIYYGVESIKKLLVFKAIFLPVATLALLFWAITQANGLDNILNQESKLHGSDFWKYF
                     FPALTGMIGFWATLSLNITDFTRYAKSQKAQITGQAIGLPPSMTLFAFVGVVVTLATT
                     IIYGQTEWDIVKLASKFESKLVVSFAMIGIIISTLATNIAANIVSPANDFANLNPKKI
                     NFKIGGYITGVVGILILPWKLIADPNGYIFTWLIAYSGLLGPIGGIMIADYFFIRKQK
                     LIVEDLYKHNGMYGYKNGFNIIALIAFIIGVIPVVPGFLVTVKLIDPNSVATWLSILY
                     HYAWFVGFFVSGILYLILTAKNAKD"
BASE COUNT          792 a          425 c          300 g          740 t
ORIGIN      
        1 ctaataccat agttgttcca ccatacaaag cagcccttgt accagtttca taactatcgc
       61 tactgaatgt acccataaag ggcatatcta aatgcacatg agggtctatg cctccaggga
      121 aaattaataa tcctgttgca tcaatttcct tatcggattg tacatttaga tttttcccaa
      181 tggcttgaac tgtttcgccg ctaataaaaa tgtcagcagt ctgatcagaa tctgctgtaa
      241 tgattcttcc gtttttaatt aatacactca taagttattt ttttaaaccg caaagggcac
      301 aaagaattac gcaatgttta caaagattaa agtccgttaa caactctttt gatgccatct
      361 tttaatctca gaacattaaa gttcatcaac aaacctaatt tataattacc aagtttcata
      421 taagtaagtg tttgtgccaa atgaatatcg tttaatgcct caacactttt tatttcaata
      481 accagttttt tttctactaa taagtctatt ctataaccgc attctaattt aacattttca
      541 aacactaatg gcattgcttt ttctttttca accaagaatc ctgagcttat caatttataa
      601 tagagacatt ccttatatgc actttccaat aagcctggac cgagagctga atgtacttca
      661 atcccattac caatcacttt atttgaaatt tcattttctg tcataaataa cttagagttc
      721 tttgcgttaa tccttagcgt tctttgcggt taatattaaa tataaaatac cactaacaaa
      781 aaaaccaaca aaccatgcat aatgatacaa gatggaaagc catgttgcaa cagaatttgg
      841 gtctatcaac tttacagtaa ctaaaaagcc tggaacaaca ggaattactc caattataaa
      901 agcaatcaat gcaataatgt tgaagccatt tttgtaacca tacattccgt tgtgtttata
      961 taaatcttct actattaatt tttgtttgcg aataaaaaaa taatctgcaa tcattattcc
     1021 tccaatgggt cccaacaaac ctgaatatgc aataagccaa gtaaaaatat atccattagg
     1081 gtctgcaatt aatttccatg gtaaaattaa aataccaaca acacctgtaa tataaccacc
     1141 aattttaaaa tttatttttt taggattaag gtttgcaaaa tcgtttgcgg ggcttacaat
     1201 attggcagct atattggttg ctaaggttga aataataatt ccaatcatag caaaacttac
     1261 tactaattta ctttcaaatt tgcttgctaa ttttactata tcccattctg tttgtccata
     1321 aattatagta gtagccaatg ttaccactac gcctacaaaa gcaaataaag tcatactagg
     1381 tggtaagcca attgcttgcc cagttatttg tgctttttgg ctttttgcat acctagtaaa
     1441 atctgtaata tttaatgata aagtagccca aaaacctatc atacctgtaa gtgctggaaa
     1501 gaaatatttc caaaaatctg agccatgtaa ttttgattcc tgattaagaa tattgtctaa
     1561 accatttgct tgagtaattg cccaaaataa taatgctaaa gttgcaactg gtaaaaaaat
     1621 agctttaaaa accaataact ttttaatact ctccacacca taataaataa tgagcatatt
     1681 taaaaaccaa aatgctaaaa aacaaataaa aggtaatgct tgagggaaca atcctgtaac
     1741 atgaatttct tctaaagttg ggttccaggt tctaattaaa ttataaatag attcgccacc
     1801 aatccatgtt tgaataccaa accatccaca agcaacaata gcccttaata tagcaggaat
     1861 attagcacct cttgtgccaa aacttgctct ggcaaaaaca ggaaaaggaa tgccatattt
     1921 tgcacctgca tggccattta aaatcattgg aattaaaaca atagagttac ctaacaaaaa
     1981 tattacaatg gcttgccacc agtttaaccc ttcttttatc ataccacttg caagcatata
     2041 agtaggaata cataaactca tacttatcca taaagctgca taattccaag taccccaagt
     2101 tcttttatcc tgtggaatag gagcaaggtc ttcgttgtat aaactattat tcatgttttt
     2161 tgggtataaa gtagtatcga attaatagtt attttaaaca caagcttcat cggcaatttt
     2221 tagtgcttcg ctaataatag ccaagccttc atctatt
//
LOCUS       DAOQPK010000305         2227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_42585_length_2227_cov_3.804328, whole genome shotgun sequence.
ACCESSION   DAOQPK010000305 DAOQPK010000000
VERSION     DAOQPK010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42585_length_2227_cov_3.804328"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PLU36_10525"
     CDS             complement(<1..702)
                     /locus_tag="PLU36_10525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HMZ47229.1"
                     /translation="MNQTGTGISKFSLESLVAAFLAGTNDAYAVIDSQFNITIFNHQY
                     SLFHKQFAYCEINEGQNYYNEINGTLLHFLIEPIQECFTSLQNKKFNHKISSTPEKFY
                     DIEIHTLINTDGKIGGVALGFFDVTQRYKVAEEIKKSELLFKSLVVNATDAFQLTNEE
                     LKITYISDSVKNVLGYEAGELLHNNFFNLIHPDDKIIISTWLHWLIHNPGVVNSVELR
                     IRNKNNEWIYIEITGN"
     gene            complement(795..>2227)
                     /locus_tag="PLU36_10530"
     CDS             complement(795..>2227)
                     /locus_tag="PLU36_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HMZ47230.1"
                     /translation="IEPEETIELNNEIYLLEGEERKEILKILKTLTVSLKPFAPLIQS
                     YLIIAGEFDFIKAKSKLALSYQGNMPNLTDKAYINIKEAYHPLLYLYNKNNHKKTTPL
                     NLKLDEKNRILIISGPNAGGKTVTMKTIGLLQLMLQSGLLVPVHPTSEMGIFKQIFIH
                     IGDTQSIEFELSTYSSHLLAMKHFIEMANGKTLFFIDELGSGSDPVLGGAFAEVILEE
                     LCKKHAMGIVTTHYLNLKVMANHTKGIINGAMAFDEKNLKPMYVLTIGKPGSSYTFAI
                     AQRIGLSPHLISKAKALVDDNHFKLDNLLNNTEKNLHALYVEKKQLQLLLKSNEKLKK
                     EMEQVINKEKHNQQIELLKNQNKISEERIVYLKEMERKLKQIVLDWKKNEDKNEVIKN
                     LNNLLFKKKEQTVVNKLAKKTNQTFKEIQNVLEVGKLVKLKKNYQVGVLKEIRGKRGI
                     VQIGQLPINVELTDLVTVEKIDTNYK"
BASE COUNT          677 a          340 c          298 g          912 t
ORIGIN      
        1 attgccggtt atttctatat aaatccattc attattttta tttcttattc ttagttcaac
       61 agagtttaca acaccagggt tatgaatgag ccaatgcagc caggttgaaa taattatttt
      121 atcatcgggg tgaattaggt taaaaaaatt attgtgcaat aattctccag cctcatatcc
      181 taatacattt tttacagaat cggaaatata ggtaattttt aattcttcat ttgttaattg
      241 aaatgcatct gtggcattta ctacaagaga tttaaaaagc agttcacttt ttttaatttc
      301 ttctgcaact ttataccgtt gtgtaacatc aaaaaagccc aatgcaacac ctccaatttt
      361 accatcagtg ttaattaagg tatgtatttc tatgtcataa aatttttcag gagtggaaga
      421 aattttatgg ttaaattttt tattttgtaa agatgtaaaa cactcttgaa taggctcaat
      481 taaaaaatga agtaaagtgc cattaatttc attgtaataa ttttgccctt cattaatttc
      541 acagtacgca aattgcttat gaaataaact gtattgatgg ttaaaaattg taatattaaa
      601 ttggctgtca attactgcat aagcatcatt agtgccagct aaaaaagctg ctacaaggct
      661 ttctaaagaa aatttactta taccagtacc agtttggttc atttagtttg caattatggg
      721 catttttttt tacaaggtta ttgtaacgta aaaattagag ataaaaatat gtggattata
      781 ttttttttaa aaatttattt ataattggta tctatttttt caactgtaac taaatctgtt
      841 agttctacat ttataggtag ctgcccaatt tgcactatac ctctttttcc tcttatttcc
      901 ttcaaaaccc ctacttgata attctttttt agttttacca gtttaccaac ttctaaaaca
      961 ttttgtattt ctttaaatgt ttgatttgtt tttttagcaa gtttattcac cactgtttgt
     1021 tctttctttt tgaagagtaa attgtttaaa ttttttataa cttcgttttt atcctcattt
     1081 tttttccagt ctaaaacaat ttgctttaat tttctttcca tttcctttaa gtaaacaatt
     1141 ctttcttcgc taattttatt ttgatttttc aataattcaa tttgctgatt atgtttttct
     1201 ttatttataa cctgctccat ttcttttttt aatttttcgt tgcttttaag caaaagctgt
     1261 aattgtttct tttcaacata caatgcatga aggttttttt cagtgttatt taaaaggttg
     1321 tctaatttaa aatgattatc atctaccaaa gcttttgctt tagaaattaa atgtggcgat
     1381 aacccaatac gttgtgcaat ggcaaatgtg taactactac ctggtttacc tatggttaat
     1441 acatacatag gttttaaatt tttttcgtca aaagccatag caccatttat tattcctttt
     1501 gtatggttgg ccattacttt taagtttaag taatgtgttg taacaatacc catagcgtgt
     1561 ttcttgcata attcttctaa aataacttct gcaaatgcac cacctaaaac tgggtcgcta
     1621 ccactgccta attcatcaat aaagaacaat gttttaccat tagccatttc aataaaatgc
     1681 ttcattgcca ataaatgact agagtaagta ctcaactcaa attctatact ttgtgtatcg
     1741 ccaatatgaa taaatatttg cttaaaaata cccatttcag atgtaggatg cactggtact
     1801 aataaaccac tttgcagcat tagttgtagt agcccaatgg ttttcattgt tacagttttg
     1861 cctcctgcat ttggcccact aataattaat attctgtttt tttcatctag ttttaaatta
     1921 agtggagttg tttttttatg gttgttttta ttgtacaaat acagtaaagg gtggtaagcc
     1981 tctttaatat taatatatgc tttatctgta aggttgggca tattaccctg atagcttaaa
     2041 gctaatttgc tttttgcttt aataaaatca aattcgcctg caataattaa atagctttga
     2101 attaatgggg caaatggttt taaactaacg gttagtgttt taagaatttt aagaatttct
     2161 tttctctctt cgccttctaa aaggtaaatt tcattattaa gttcaatggt ttcttctggc
     2221 tcaataa
//
LOCUS       DAOQPK010000306         2223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_42725_length_2223_cov_3.596402, whole genome shotgun sequence.
ACCESSION   DAOQPK010000306 DAOQPK010000000
VERSION     DAOQPK010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42725_length_2223_cov_3.596402"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2154
                     /locus_tag="PLU36_10535"
     CDS             <1..2154
                     /locus_tag="PLU36_10535"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HMZ47231.1"
                     /translation="IKLPTNCNNIEYKYGVFDIEKKQFVHYEDGDNRVLNKQLCFDDL
                     TIVHDGFVQLPFNSWKGAGVNIPVFSLRSENSFGIGEFYDLKLLAKWAAKVSLKLIQI
                     LPVNDTTENFSDSDSYPYAAISAFALHPIYICLDELIDKSDKVIVNNFNSLKKKLNQS
                     SQVSYSMVLKHKWHYLQQIFTKKHTLVFATKEYQTFYQKNKFWLIPYAAFCFLRNKYG
                     TANFALWKEFAIYNEKKIALLVSEKGKHFKEVSIHFFVQFYLHKQLSEAVQEANKLGI
                     ILKGDIPIGINKNSVDCWQYPQLFNCNMQAGAPPDDFTEKGQNWGFPTYNWQVMQEDN
                     FLWWKLRFQQMQCYYDAFRIDHILGFFRIWSIPSNAVEGVMGYFVPAIPLQLNDFNKS
                     GIFFNYVRYTQPFITDEILESFFDEYKEEVINQFLIKDDFSDNYTLTEKFSTQILVEN
                     FFSALPNNTKNNKIKQGLFNLISNVILLDIDGNENEFHFRFNMFKTTSFKQLSPIVQQ
                     QLLKLYNNYFFEQQNELWKKEGIYKLTTIKCATNMLVCGEDLGLVPAIVPEVMKSLGI
                     LSLEIQRMPKQNQQHFLQLNQLPYLSVAMPSTHDMSTIRSWWQEDFTNTKLFYNTVLN
                     KTNHLPQECTVDISKEIIKQHLYSSAMWSIFLLQDLFGIDENLRIENHQQERINNPAN
                     KNQIWNYRMHISLEKLLRQFTFNAELKKQIQLSGR"
BASE COUNT          834 a          275 c          342 g          772 t
ORIGIN      
        1 attaaactac caacaaattg taataatata gagtataaat atggtgtttt tgatattgaa
       61 aaaaaacaat ttgtacatta tgaagatgga gataatagag tattaaataa acaactttgt
      121 tttgacgatt taaccatagt gcatgatggc tttgtacaac ttccttttaa ttcatggaaa
      181 ggtgcagggg taaatattcc tgtatttagt ttaagatctg aaaatagttt tggtataggt
      241 gagttttatg atttaaaact attggcaaaa tgggctgcta aggtatctct taaattaatt
      301 caaattttac ctgttaacga cactacagaa aatttctctg actcagattc ttatccttat
      361 gctgccattt cagcctttgc tttgcatcct atttatattt gtttagatga gttaatagat
      421 aagtcagata aggttatagt aaacaatttt aactcattaa aaaagaaact aaatcaatct
      481 tctcaagtta gctattcaat ggttttaaaa cataaatggc attacttaca acaaattttt
      541 actaaaaagc atacattggt ttttgctact aaagagtatc aaacattcta ccaaaaaaat
      601 aaattttggt taatacctta tgctgctttt tgttttttaa gaaacaaata tggaacggcc
      661 aattttgcac tgtggaaaga atttgctatt tataatgaaa aaaaaattgc tctattagtt
      721 tctgaaaaag ggaaacattt taaagaagta tccattcatt tttttgttca attttattta
      781 cataaacaat tatcagaagc tgtacaagag gcaaataagt taggcattat tttaaaagga
      841 gatattccaa ttgggataaa taaaaatagt gttgattgtt ggcaataccc acaattattt
      901 aactgcaata tgcaagcagg agcacctcca gatgatttta cagagaaagg gcaaaactgg
      961 ggctttccta cttataattg gcaagtaatg caagaagata attttttgtg gtggaaacta
     1021 aggtttcaac aaatgcaatg ttattatgat gcttttagga tagatcatat tttaggtttt
     1081 tttagaattt ggagtattcc atctaatgct gtagaaggtg taatgggtta ttttgtacct
     1141 gcaattcctt tgcaattaaa tgactttaat aaaagtggta ttttttttaa ttatgtaaga
     1201 tatacccaac cttttataac agatgaaatt cttgagtctt tttttgatga atataaagag
     1261 gaagtaatta atcagttttt aataaaagat gatttttcgg ataattatac tctcactgaa
     1321 aaattttcaa ctcaaatatt agtagaaaat ttcttttctg ctttgcccaa taatactaaa
     1381 aacaataaaa ttaaacaagg tttatttaat ttaataagta atgttatttt gttggatata
     1441 gatggaaatg aaaatgagtt tcactttagg tttaacatgt ttaaaacaac ttcttttaaa
     1501 caactttcac caattgtaca acagcagtta ttgaagttgt ataacaacta tttttttgag
     1561 caacaaaatg agttatggaa gaaagaaggg atttataaac taacaacaat taaatgtgca
     1621 accaatatgc ttgtttgtgg cgaagattta ggtttagtgc cagcaattgt tccagaggta
     1681 atgaaaagct taggcatttt aagtttagaa attcaaagaa tgcctaaaca aaatcagcaa
     1741 cattttttac aacttaatca actaccatat ttaagtgttg caatgccctc tacacacgac
     1801 atgagtacaa taagaagttg gtggcaggag gactttacta atactaaact tttttataat
     1861 actgtattaa ataaaactaa tcatttgccc caagagtgta cggttgatat tagtaaagaa
     1921 attattaaac agcatttata ttcttctgca atgtggagca tttttttact acaagattta
     1981 tttggaatag atgaaaattt gagaattgaa aaccaccaac aagaaagaat aaataaccca
     2041 gccaataaaa atcaaatatg gaattataga atgcacatca gtttagaaaa gttattacga
     2101 cagtttactt ttaatgcaga gttaaaaaag caaattcaat taagtggaag ataagtttat
     2161 aaatatcttt cttttaaaac atctatatgg tgtaaaatat gagcataaat aataaaaaca
     2221 att
//
LOCUS       DAOQPK010000307         2216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_42979_length_2216_cov_5.111060, whole genome shotgun sequence.
ACCESSION   DAOQPK010000307 DAOQPK010000000
VERSION     DAOQPK010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42979_length_2216_cov_5.111060"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2058)
                     /locus_tag="PLU36_10540"
     CDS             complement(<1..2058)
                     /locus_tag="PLU36_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47232.1"
                     /translation="MKQNLKKLIFSCLLITGISTSKLAMAQSPCTTTTNSCNYLSNGG
                     FENMDGLNCDDLHATGASVYCIGNNLFTDNTFHGCSWTAGGWTYSGTAQTADYFNACA
                     STCWVPGVNSTDNHGSNGIGSISPHDGNGYAGIFVYTSATAFPGGSASYKESIKQTLS
                     TPLIAGKTYIVSMWVRLAHISKYAVSDFWLNGINFTSSPITDKNNWTFLSTCYTPTAT
                     VTQIEIGSNATTLTDLDPTGTSYGTNYGQLNTVSYYYIDDVSIKPFNVNAGPDIISLF
                     PGCGTQIGTANGGYSCLPAGATIAYNWSPSTGLSASNVATPIASPNATTNYTVTATVT
                     YTNEAGVTAVCSSTDAVLVDVVTPTINITPSFTCNFNGVSSFTATPSPAGSYTYSWTV
                     KDVATGTVVAVAGSGTTSATPSFSLHNVIRPVNICVSITNIYGCISTPTCYYLPNCCP
                     TGTNIINYANTTYSTSTVLASATGTNAIVLSGTITVNSGGTLIISSKEVLMNPNTKIV
                     LNGSGKISIASDYVHGCNYMWDGIYANGSTSGVTINNSRVEDAKRVVIDSLGGASVTL
                     LNSYLNKNNMGIVFKATKTSASVVSIKNTLLTCASISTAGIVPKIPTAVNLTNAVTLG
                     AFTSTVMLPPYNNQKSYCGIYFENASHTGKANAAITIGGTTGQENVFDKMQHGAFVNI
                     SKAN"
BASE COUNT          773 a          385 c          418 g          640 t
ORIGIN      
        1 attagctttt gagatattta caaaagcacc gtgttgcatt ttatcaaata cattttcttg
       61 cccggtagtt ccaccaatag taattgcagc attggcctta cctgtatgcg aagcattttc
      121 aaagtaaata ccacagtaag acttttgatt attgtatgga ggaagcatca cagtagaagt
      181 aaaagcacct aaagttaccg cgttagttaa gttaacagct gtaggtattt taggaacaat
      241 gcctgcagtt gaaatgcttg cgcaagtaag taaagtgttt ttgatactta caacagatgc
      301 agaagttttg gttgctttaa aaacaatacc catattattt ttattgaggt agctattaag
      361 tagtgtaacc gaagcaccac ccaaggaatc aataacaaca cgttttgcat cttctacacg
      421 ggaattatta atagttacac ctgatgtgct gccatttgca taaataccgt cccacatgta
      481 attacatccg tgaacataat cactagctat tgaaatttta ccagaaccat ttaaaacaat
      541 tttggtatta gggttcataa gaacttcttt ggaactgata attaatgttc cgcctgaatt
      601 aacagtaata gtaccactta aaacaatagc attagtgcct gttgcagaag ctaaaacagt
      661 agaagttgaa tacgttgtgt ttgcatagtt aattatatta gtacctgttg gacagcaatt
      721 tggtaaatag taacaagtag gtgtggaaat gcaaccataa atattagtaa ttgaaacaca
      781 aatattgaca gggcgaataa cattatgtag actaaaagaa ggagttgcgg atgttgtgcc
      841 agagccagca acagctacaa ctgtgccagt agccacatct ttaaccgtcc agctgtatgt
      901 ataagaacca gctggactag gagtagccgt aaaactacta acgccattaa aattacaagt
      961 aaagctcgga gtgatattaa tagtaggagt aacaacatct actaaaactg catcagtaga
     1021 actacaaacc gcagttacac cagcctcgtt agtataagtt acggttgctg taacagtgta
     1081 attagtagta gcattagggc ttgcaatagg agtagctaca tttgaagcag ataaaccagt
     1141 gcttggggac cagttatacg caattgttgc accagcagga agacaagaat acccaccatt
     1201 agcagtgcca atttgagtgc cgcaaccagg aaataaagaa ataatatcag ggcctgcgtt
     1261 aacattaaaa ggtttgatag aaacatcatc aatataatag taagaaaccg tatttagttg
     1321 accataattt gtaccataag aagtaccagt aggatcaaga tcagttaaag tagtagcatt
     1381 agaaccgatt tcgatttgag taacggttgc cgttggagta taacaagtag aaagaaaagt
     1441 ccaattattt ttgtctgtaa taggagaact agtgaaatta ataccattta accaaaaatc
     1501 agaaactgca tatttactaa tatgagccag gcgaacccac atacttacaa tataagtttt
     1561 acctgcaatc agtggcgtac tcaatgtttg tttaattgat tctttataag aagcagagcc
     1621 cccaggaaat gcagtagcag aagtataaac aaatatacct gcataaccat taccatcatg
     1681 aggcgatatt gagccaatac cattgctacc atgattatct gtagaattaa cgcccggcac
     1741 ccaacaagta gaagcgcaag cgttaaaata atcggctgtt tgtgctgtcc cactatatgt
     1801 ccagccacct gctgtccagc tacaaccatg aaatgtgtta tcggtaaata aattattacc
     1861 tatacaataa acagaggcac ctgtagcgtg taaatcatcg cagtttaaac catccatatt
     1921 ttcaaaccca ccatttgaaa gatagttaca ggaatttgta gttgttgtgc atgggctttg
     1981 agccatggca agttttgaag ttgaaatgcc cgtaatgagc aagcaactaa agattaattt
     2041 tttaagattt tgtttcatta attttttgtt ttaataattt gtgttttagg tttaattgat
     2101 tgtgtgcgaa aatatcattt ataagttcat ttacagtgac gataaaaaaa taacattact
     2161 gatgaaagaa attttaaaaa atatataaat aataccgagc tgccgcaggc gagcgc
//
LOCUS       DAOQPK010000308         2208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_43285_length_2208_cov_4.313052, whole genome shotgun sequence.
ACCESSION   DAOQPK010000308 DAOQPK010000000
VERSION     DAOQPK010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43285_length_2208_cov_4.313052"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /locus_tag="PLU36_10545"
     CDS             complement(<1..1261)
                     /locus_tag="PLU36_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012791086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMZ47233.1"
                     /translation="MKTETLSQAHEIIQDAQDYTLFSWSKQKGTNPIAIKHAEGVYLY
                     DYDDNKIIDFSSGLINMNIGYGNQRITNAVVQQMQEVSYVSPSCVTKVRADLGKKLAE
                     ICPGDLNKSFFTLCGATSIENGIKLARLYTGRHKIVTRYQSYHGATMGAISAGGDPRR
                     LPVDAQQAPNFVHFDLPYLYRWEYGEENLLKEAVASFERIIAYEGPTNIAAVLLEGES
                     GTSGCLKYPKGFLKAVREITQKHGILLIIDEVMSGFGRTGKWFGFQNHDIVPDMIAMA
                     KGLTCGYMPLGCLQVSDKIAAKYDDTVLPLGLTYSAHAVSCAAALETIKVYEEDNLFE
                     NAIAMGKYMEQQIEKLKEKHPSIGDFRNTGLLGCMELVKNRKTKEPLAPFNAKPTEME
                     VMNKVAAKIKALGMYTFVRWNFIFCAPP"
     gene            complement(1321..>2208)
                     /locus_tag="PLU36_10550"
     CDS             complement(1321..>2208)
                     /locus_tag="PLU36_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMZ47234.1"
                     /translation="KEAGLPDGVFNVVHGDVEIVEAICDHPGIEAVSFVGSTKVAKIV
                     YQRATQNLKRCLALGGAKNHLLVLPDAIPDMTAQNVAASMSGCAGQRCMAASAMVAVG
                     SVQHIIDKICEEAKKIIPGKNLGAVINKESQLRIEKYITEAEQQGAKILVDGRNTKVE
                     GQEYGTYVGPTVIDYVTPEMSVAKEEIFGPVISIMRTKTVDEALAIENANPYGNAASV
                     FTQNGGMARYIIDKASAGMIGVNVGVPVPREPFSFGGWNESKFGVGDITGKSSIEFWT
                     KLKKSTTKWNAEAGINWMS"
BASE COUNT          644 a          461 c          302 g          801 t
ORIGIN      
        1 atggtggtgc acaaaaaata aaattccaac gtacaaaagt gtacatgcct aacgctttta
       61 ttttagcagc cactttattc ataacttcca tttcggttgg tttggcatta aatggagcta
      121 aaggttcttt agtttttctg ttttttacta attccataca ccctaataaa ccggtatttc
      181 taaagtcgcc aatagatgga tgtttttctt ttagtttctc tatttgttgc tccatgtatt
      241 tgcccattgc aattgcattt tcaaacaaat tatcttcttc gtacacttta attgtttcta
      301 aggctgcagc acaacttact gcatgagctg aataagttaa accaagtggt aaaacagtgt
      361 catcatattt agcagctatt ttatcgctaa cttgtaagca acctaaaggc atataaccac
      421 aagttaaacc ttttgccata gcaatcatat caggaacgat atcatgattt tgaaacccaa
      481 accatttacc tgttctacca aagccactca ttacttcatc tataattaat aatattccat
      541 gcttttgggt aatttctctt acagctttta aaaatccttt tggatatttc aagcagcctg
      601 atgtgccaga ttctccttct aataaaactg ctgcaatgtt tgttggtcct tcataagcaa
      661 taatacgttc aaacgatgct acagcttctt tcaataaatt ttcttcacca tattcccatc
      721 ggtataaata aggtaaatcg aagtgaacaa aattgggtgc ttgctgagca tctactggta
      781 atcttcttgg gtcgccacct gcagaaatag cacccattgt agcaccatga taagattgat
      841 aacgtgtaac aattttatga cgacctgtat ataaacgtgc taatttaatt ccattttcta
      901 tagaggttgc accacataaa gtaaagaacg atttgtttaa atctccagga caaatttctg
      961 ctaatttttt tcctaaatct gctcttactt tagttacaca acttggcgaa acatacgata
     1021 cttcttgcat ttgttgtaca actgcattgg ttattctctg gttgccatag ccaatattca
     1081 tatttattaa tccactagaa aaatctatta ttttattatc atcataatcg tataaataaa
     1141 caccttctgc atgtttaatg gcaataggat ttgtgccttt ttgtttactc caactaaaca
     1201 aagtataatc ttgagcatcc tgaattattt catgagcttg tgataaggtt tctgttttca
     1261 ttttttatat tgattatcgt tattaattaa tatttaaata cgctacgctt tcaggtttat
     1321 ttaactcatc cagttgatgc ctgcttctgc attccatttt gtagtagatt tttttagttt
     1381 agtccaaaac tcaatactac tttttcctgt aatatcgcct acgccaaatt tgctttcatt
     1441 ccatccgcca aaactaaatg gctcacgtgg tacaggtaca ccaacattta caccaatcat
     1501 tcctgcactt gctttatcaa ttatataacg tgccatacca ccattttgtg taaacacact
     1561 tgcagcattg ccataagggt ttgcattttc tattgccaaa gcttcatcta ctgttttagt
     1621 acgcatgata gaaataactg gcccaaaaat ttcttctttg gcaacactca tttctggtgt
     1681 tacatagtca ataactgttg gaccaacata agttccatac tcttgtcctt ctacttttgt
     1741 atttctgcca tctactaaaa tttttgcacc ttgttgctct gcttcagtta tatacttttc
     1801 aattcttaat tgcgattctt tattaattac agcacctaaa tttttgcctg gaattatttt
     1861 ttttgcttcc tcacaaattt tatcaataat atgttgtaca ctgcctacag caaccattgc
     1921 acttgctgcc atacaacgtt gtcctgcaca accactcata cttgcagcaa cattttgtgc
     1981 agtcatatca ggtattgcat ctggtaaaac caataaatga ttttttgcac ctcccaatgc
     2041 caaacatctt tttaaatttt gtgttgcccg ttgataaaca attttagcca ctttggtaga
     2101 acctacaaaa gaaactgctt ctatgccagg atggtcgcaa atagcttcta caatttctac
     2161 atcgccatga actacattaa atacaccatc aggtaaccct gcttcttt
//
LOCUS       DAOQPK010000309         2204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_43447_length_2204_cov_4.096789, whole genome shotgun sequence.
ACCESSION   DAOQPK010000309 DAOQPK010000000
VERSION     DAOQPK010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43447_length_2204_cov_4.096789"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(167..1267)
                     /locus_tag="PLU36_10555"
     CDS             complement(167..1267)
                     /locus_tag="PLU36_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HMZ47235.1"
                     /translation="MTIQDIINVLENYAPPALQESYDNVGLLIGNKNWNCTGIICCLD
                     STEDVIKEAISKKCNLIVAHHPIIFSGLKKLNGSNYVEQTIIKAIKNDIAIYAIHTNL
                     DNLIDGVNKSIADKLNLKNTSILLPKNNMLQKLYTFVPINYAEKVKEALFAVGAGEIG
                     NYSECSFNTKGIGTFNANQLSKPFVGNKGLRHEEVEIKVEIIYPIWLKSKIINTLKNV
                     HPYEEPAFDIISLQNQYNKIGSGLIGELAKPISAKMFLQNLSKVFDLQIIRHSKLNKK
                     NIKKVAICGGAGNFLTTFAMQASADIFITSDIKYHEFFDTNSSMILADIGHFESEQFT
                     IELLQNYLHKKFPNFAVLKTKVKTNPVEYFIV"
     gene            complement(1407..>2204)
                     /locus_tag="PLU36_10560"
     CDS             complement(1407..>2204)
                     /locus_tag="PLU36_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HMZ47236.1"
                     /translation="MVKGTLPIPAINVNDSVTKSKFDNKYGCKESLVDSIRRATDVMM
                     AGKVAVVGSYGDVGKGSAASLKGAGCRVIVTEIDPICALQAAMDGFEVKPMIKAVAEA
                     DIIVTASGCRDLITEKHFRLMKDKAIVCNIGHFDIEIDMAWLNDNFGHTKNTIKPQVD
                     LYTIDGKDVIVLAEGRLVNLGCATGHPSFVMSNSFTNQTLAQIELWTNTDKYENKVYV
                     LPKHLDEKVARLHLAKIGVELDVLSDEQAAYLGIPKNGPFKAEHYRY"
BASE COUNT          740 a          365 c          282 g          817 t
ORIGIN      
        1 gtaagaactg aaaccagttt ttcctgtact gaaaattctt taactgctgc catatactta
       61 tttaaagttt acatttttgt gatagtgggt tctgcaatta cttaaagtag ttattacatt
      121 actcacttgt actatttatt aacaaagttg ctaaaacatt attgtattaa actataaagt
      181 attctacagg gtttgtttta accttagttt taaggacggc aaagttaggg aattttttgt
      241 gtaaataatt ttgtaataat tctatagtaa attgttcact ttcaaaatgt ccaatatctg
      301 ccaaaatcat ggaagaattt gtatcaaaaa attcatgata ttttatatca gatgttataa
      361 aaatatctgc acttgcttgc atagcaaaag ttgttaaaaa gttgccagca ccaccacaaa
      421 ttgctacttt tttaatattt tttttattta atttactatg cctaattatt tgtaaatcaa
      481 atactttaga taaattttgt agaaacattt tagcactaat tggttttgct aattcaccaa
      541 ttaaaccact ccctattttg ttatattggt tttgtaaact aataatatca aaagcaggct
      601 cttcgtaagg gtgtacattt ttcaatgtat ttattatttt acttttaagc caaataggat
      661 aaataatttc tacttttatt tctacttctt catgccttaa tcctttattt cctacaaatg
      721 gtttagacag ttgattagca ttgaaagtac caataccttt tgtgttaaaa ctacactcac
      781 tataattgcc tatttcgcca gcccctactg caaacaaagc ttctttcact ttttcggcat
      841 aatttattgg cacaaaagtg tacagttttt gcagcatatt atttttgggt aataaaatag
      901 aagtattttt taaatttaat ttatcggcaa ttgatttgtt tacgccatct attaagttat
      961 ctaaattggt atgaattgca taaatggcaa tatcattttt aatagcttta ataattgttt
     1021 gttcaacata attactacca ttaagctttt ttagaccact aaaaattatt ggatgatgtg
     1081 caactattaa attgcatttt ttggatattg cttcttttat aacatcttct gtagaatcaa
     1141 ggcaacaaat aattcctgta caattccaat ttttatttcc tattaataat ccaacattat
     1201 cataactctc ttgtaatgct ggtggagcat aattctctaa aacattaata atatcttgta
     1261 tagtcatttg ctacaaagct ataagaagta atttaaaaat ataaagtatt ggcttattta
     1321 ttcaataata ccaccaccct attaataata taacaaaaaa ggtacaatta cattgtacct
     1381 ttaatagtaa gtgtatgtga gaaagattaa tacctgtaat gctctgcttt aaaagggcca
     1441 tttttaggaa tacctaaata agcagcttgt tcatcagata atacatcaag ttcaacacca
     1501 atttttgcaa ggtgcaaacg tgctactttt tcatcaagat gctttggtaa aacataaact
     1561 ttgttttcat atttatcagt atttgtccaa agttctatct gagctaaagt ttggttggta
     1621 aatgaattac tcattacaaa acttgggtgt cctgttgcac aacctaaatt tactaaacga
     1681 ccttcggcta aaactattac atctttacca tcaatagtgt aaagatctac ttgaggttta
     1741 atggtattct ttgtatgacc aaaattgtca tttaaccaag ccatatcaat ttcaatatca
     1801 aagtgcccaa tgttacaaac aatagctttg tctttcatta atctaaaatg tttttcagta
     1861 attaaatcac gacaaccaga agcagtaact ataatatcag cttctgctac agctttaatc
     1921 attggtttta cttcaaagcc atccatggca gcttgtaaag cacaaattgg gtctatttca
     1981 gtaacaatta cacgacaacc agcacccttt aatgaagcag cagaaccttt acctacatct
     2041 ccataagaac caacaacagc aacctttcca gccatcataa catctgtagc tctacgaata
     2101 gagtcaacta aagattcttt acagccatat ttgttatcaa atttagattt agtaacactg
     2161 tcgtttacat taattgcagg aattggtaat gtgcctttta ccat
//
LOCUS       DAOQPK010000310         2191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_43920_length_2191_cov_2.964888, whole genome shotgun sequence.
ACCESSION   DAOQPK010000310 DAOQPK010000000
VERSION     DAOQPK010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43920_length_2191_cov_2.964888"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..1070)
                     /locus_tag="PLU36_10565"
     CDS             complement(18..1070)
                     /locus_tag="PLU36_10565"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HMZ47237.1"
                     /translation="MNQNNRFTDSGIEINKVYTKEDLPSSLTKEMPGDFPFTRGVQAD
                     MYRGKLWTMRQYAGFSTAEESNKRYHYLLSQGVMGLSVAFDLPTQIGYDSSHELAEGE
                     VGKVGVAIDSLEDMEQLFDGITLENVSTSMTINATGFILLAFYVALAKKQGADLKKIS
                     GTIQNDILKEYAARGTYIYPPKPSMRIITDIFEWCSKELPKWNTISISGYHIREAGST
                     AVQEIAFTLSNGKAYVKAALEKGLDINVFGKRLSFFFNAHNNLFEEVAKFRAARKMWA
                     TIMKDLGATDEKALMLRFHTQTGGATLTAQQPHNNICRVTIQTLAAVLGGTQSLHTNG
                     FDEALSLPTENADACA"
     gene            1219..2094
                     /locus_tag="PLU36_10570"
     CDS             1219..2094
                     /locus_tag="PLU36_10570"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HMZ47238.1"
                     /translation="MSTKEELLQDVVIKFAGDSGDGMQLTGQQFTNNTALLGIDLATF
                     PDFPAEIRAPIGTLPGVSGFQLHFSSERVFTPGDTCDVLVAMNAAALKVNLRSLKKGG
                     KIIANTDGFDTKNLRLANYKDGDNPLENGSLEGYELIKIDVTKITREALKDFAGMGTK
                     ERDRTKNMFVLGFVYWMYNRSLDSTIAFLKEKFGKNETILESNLKVLQAGYNYGDTTE
                     TFSSRYKVEKAKMPNGSYRSIMGNQALAYGLIAASNKSGLPMFLGTYPITPASDILHE
                     LSKHKNFGIKTFFDN"
BASE COUNT          755 a          370 c          350 g          716 t
ORIGIN      
        1 aatcttcatg aaaattccta agcgcacgca tcagcatttt ctgtaggtaa gcttaaagct
       61 tcatcaaaac cattggtatg taaactctgt gtaccaccta aaacagcagc aagtgtttgt
      121 atggtaactc tgcaaatatt attatgcggt tgttgtgctg ttaaagttgc cccaccagtt
      181 tgtgtatgaa acctaagcat taaagccttt tcatcggttg cacctaaatc tttcattata
      241 gtagcccaca tttttctggc tgctctaaac tttgcaactt cttcaaataa attgttatgg
      301 gcattaaaaa agaaagataa tcttttgcca aaaacattta tatctaatcc tttttctaat
      361 gcagctttta cataagcctt tccattactt aaggtaaaag caatttcttg cacagcagtg
      421 ctgcctgctt ctctaatatg ataacctgat atagaaatag tattccactt aggtaattct
      481 ttactgcacc attcaaatat atctgttata atacgcattg atggcttagg aggataaata
      541 taagtgcctc tagcagcata ctcttttaaa atatcgtttt gtatggtacc cgaaattttc
      601 tttaaatctg ctccttgttt ttttgctaat gcaacataaa atgccaataa aataaaacca
      661 gttgcattaa tagtcataga ggtggataca ttttctaaag taataccatc aaatagttgc
      721 tccatatctt ctaaactatc aattgctaca ccaactttac caacctcccc ttcagccaat
      781 tcatggctgc tatcataccc aatttgagta ggtaaatcaa atgctacact taaacccatt
      841 acaccttgag ataacaaata gtgatagcgt ttattgcttt cttcggctgt tgaaaaacca
      901 gcatactgcc tcatcgtcca aagttttcct ctgtacatat ctgcttgcac ccctctggta
      961 aaaggaaaat caccaggcat ttctttggta agtgaagaag gtaaatcttc tttagtatat
     1021 actttattta tttcaatacc actatcagta aatctgttat tttggttcat ataaaacaat
     1081 ttgttttaca actttttcag ctaaatattt tctatttcag taaaagttca aaggtaaaag
     1141 ataaaatagt aaaaaagtaa cttcatttaa tcaatagcat atagctttgt aacagtttca
     1201 aatcttaaat attattctat gagtacaaag gaagaattat tgcaagatgt agtaataaaa
     1261 tttgcaggag atagtggaga tggtatgcag ttaacaggcc agcagtttac taataatact
     1321 gcattattag gaattgactt agcaactttc ccagattttc ctgcagaaat aagagctcct
     1381 attggtacac taccaggtgt tagtgggttt cagttacatt ttagtagcga aagggttttt
     1441 actcctggag atacttgcga tgtactagtt gctatgaatg ctgcagcttt aaaagtaaac
     1501 ttaagaagcc taaaaaaagg cggaaagatt atagctaata cagatggctt tgatacaaaa
     1561 aatttaagat tagccaatta taaagatgga gacaaccctt tagaaaatgg aagtttagag
     1621 ggatatgaac tgataaaaat agatgtaacc aaaattacaa gagaggcctt aaaagatttt
     1681 gcaggcatgg gtacaaaaga aagagataga actaaaaata tgtttgttct tggctttgta
     1741 tattggatgt ataatagaag tttagatagt accattgcct ttttgaaaga aaagtttgga
     1801 aaaaatgaaa ccattttaga aagtaaccta aaagtattac aagcaggcta caattatggc
     1861 gatactacag aaactttttc atcaaggtat aaagtggaaa aagctaaaat gccaaacggt
     1921 tcttacagaa gcattatggg caaccaagca ctggcttatg gcttaatcgc agctagtaat
     1981 aaaagtgggt taccaatgtt tttaggcacc taccctatta ctcctgcttc agatatttta
     2041 catgaactaa gcaaacacaa aaattttggt attaaaactt tttttgacaa ctaaaaaatt
     2101 gctaaataat ctgacattat ttttgttgca actccatcta ctctggataa ttggatcatt
     2161 gttgatataa aaaaacatat ttacattttt t
//
LOCUS       DAOQPK010000311         2167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_44835_length_2167_cov_3.163352, whole genome shotgun sequence.
ACCESSION   DAOQPK010000311 DAOQPK010000000
VERSION     DAOQPK010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44835_length_2167_cov_3.163352"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PLU36_10575"
     CDS             <1..526
                     /locus_tag="PLU36_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ47239.1"
                     /translation="KLREQISYVPQDVFLFSDSIKNNINFGLSNEAEMELIQNVAKLA
                     SVHNDILEFENGYETIIGERGVTLSGGQKQRISIARALLKSPEIIVFDDCLNAVDAKT
                     ENTIIQNFYTFLQNKTAIIITHRIFSSFKFDKILVLNEGKIIEAGTHETLMALNGYYA
                     ALYKKQLTEEENNL"
     gene            720..>2167
                     /locus_tag="PLU36_10580"
     CDS             720..>2167
                     /locus_tag="PLU36_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HMZ47240.1"
                     /translation="MEIRNIAIIAHVDHGKTTLVDKILHATKIFRENQDSGELIMDNN
                     DLERERGITIFSKNAAVKYNGVKINVIDTPGHSDFGGEVERVLKMADGVILLVDAFEG
                     PMPQTRFVLQKALQLNLHPIVVINKVDKPNCRPDEVHDAVFELFFNLDATEEQLNFPT
                     FYGSGKNGWFNDSLTPTDNILPLMDGILKYVPAPKVAEGALQLQITSLDYSSFLGRIA
                     IGKVTRGSIKENMPIALVQADGTIKKSRVKELYVFEGMGKSRVNEVKAGDLCAVVGLE
                     DFNIGDTIADAENPEALPIISVDEPTMSMTFSINNSPFFGKDGKFVTSRHLRDRLMKE
                     TEKNLALRVEDTDSADSFLVYGRGILHLGVLVETMRREGYELTVGNPQVIVKQINGKK
                     HEPYEVLVVDVPSEYSGKVIDLVTQRKGEMLVMESKGEMQHLEFDIPSRGLIGLRSQM
                     LTGTAGEAVMAHRFSEYKPWKGSIPGRNNGVL"
BASE COUNT          791 a          299 c          396 g          681 t
ORIGIN      
        1 taaactaaga gaacaaatta gttatgttcc acaagatgtt tttttattta gcgatagtat
       61 aaaaaacaat ataaactttg gtttaagtaa tgaggcagaa atggaattaa tacaaaatgt
      121 agcaaaatta gcttctgttc acaacgatat tttagagttt gaaaatggat atgaaactat
      181 tattggcgaa agaggtgtta cattaagtgg tggtcaaaag caaagaattt ccattgctag
      241 agctttactt aaaagccctg aaataattgt ttttgatgat tgcttaaatg ctgttgacgc
      301 taaaactgaa aatactatca tccaaaattt ttataccttt cttcaaaata aaactgcaat
      361 aattataacc catagaatat tttcatcttt taaatttgat aaaattttag ttcttaatga
      421 agggaaaatt atagaagctg gcactcatga aactcttatg gctcttaatg gatactatgc
      481 agccttatat aaaaaacagt taaccgagga ggaaaataac ttataacaac tttcttcaaa
      541 acatttacct tcgttagcta taataaatta attatcaata ttttaaaact ttttatttac
      601 caaaacaata gaaagcccta acttcgcagc cctaaaattt cacttcgcct tttataaaag
      661 cttggtttat tttatttttt actaattcta aaaaattaaa gtcataatta gactactata
      721 tggaaatacg caacattgca attattgcac acgtagatca tggtaaaaca actttggttg
      781 ataaaatttt acatgcaact aaaattttca gagaaaacca agactcaggt gagttaataa
      841 tggataataa tgatttagaa agagaaagag gaattaccat ttttagcaaa aatgctgctg
      901 ttaaatataa tggcgttaaa ataaatgtaa tagatacccc aggccacagt gattttggcg
      961 gtgaggttga aagagttttg aaaatggctg atggggtaat acttttagta gatgcttttg
     1021 aaggtccaat gcctcaaact cgttttgtat tgcaaaaagc attacaatta aacctacatc
     1081 ctattgtagt tattaataaa gtagataaac caaactgccg cccagatgaa gtgcatgatg
     1141 cagtttttga acttttcttt aatttagatg caaccgaaga acaattaaat tttcctacat
     1201 tttatggtag tggaaaaaat ggctggttta atgatagctt aacacctaca gataacattt
     1261 tgcctttaat ggatggtatt ttaaaatatg ttcctgctcc taaagttgct gaaggtgcat
     1321 tacaacttca aataacttct ctcgattatt cttctttctt aggaagaatt gctattggta
     1381 aagttaccag aggctctata aaagaaaata tgcctattgc attagtgcag gcagatggaa
     1441 caattaaaaa aagccgtgta aaagaattat atgtttttga aggaatgggc aaaagcagag
     1501 taaatgaagt aaaagctggt gatttatgtg ctgtagttgg cttagaagat tttaatattg
     1561 gcgataccat tgctgatgct gaaaaccctg aagcattacc aattataagc gttgatgagc
     1621 ctactatgag catgactttt agtattaaca actcaccctt ttttggtaaa gatggaaaat
     1681 ttgttacaag ccgtcactta agggataggt taatgaaaga aacagaaaag aatttagctt
     1741 taagagtaga agatacagat agtgcagata gttttttagt atatggcaga ggcatattac
     1801 atttaggtgt tttagtagaa actatgcgta gagaagggta tgaattaaca gttggtaacc
     1861 ctcaagtaat tgtaaaacaa attaatggta aaaaacatga accttacgaa gtattagtgg
     1921 tagatgtacc aagtgaatac agtggtaaag taatagattt agtaacacaa cgtaaaggag
     1981 aaatgttggt aatggaaagc aagggagaaa tgcaacattt agaatttgac atcccttcaa
     2041 gagggttaat aggtttacgt tctcaaatgc ttactggcac tgctggcgaa gctgtaatgg
     2101 cacatcgttt tagcgaatac aaaccatgga aaggatctat cccgggtcgg aacaacggtg
     2161 tgctgat
//
LOCUS       DAOQPK010000312         2153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_45369_length_2153_cov_4.022402, whole genome shotgun sequence.
ACCESSION   DAOQPK010000312 DAOQPK010000000
VERSION     DAOQPK010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45369_length_2153_cov_4.022402"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..1149
                     /locus_tag="PLU36_10585"
     CDS             157..1149
                     /locus_tag="PLU36_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HMZ47241.1"
                     /translation="MNSFGKIFKVQIFGESHGNCVGVVIDGVTAGLPLNEEDFLSDLV
                     RRQGGIQKGTTPRKEIDVPIFKSGIFKDKATGTPLTILFENNNIQSEVYDKQRSIPRP
                     GHVDFTANKKFGGYEDYRGGGHFSARLTTGLVAAGVVAKKMMPEININAVVVEIAGER
                     DLEKGIQKAINEKDTVGGIIECKVLGLPIGLGEPYFDSLESLLAHILFSIPAIKGVEF
                     GSGFNAAKMFGSEHNDAIENKEGKTSSNFAGGIVGGLSNGNELVFRIAVKPASSTPKE
                     QESLNWETNTMEKFSIQGRHDLCVALRAPVIVEAVTAIVLADCMLLEQKIQRVL"
     gene            complement(1144..1827)
                     /gene="radC"
                     /locus_tag="PLU36_10590"
     CDS             complement(1144..1827)
                     /gene="radC"
                     /locus_tag="PLU36_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HMZ47242.1"
                     /translation="MAGIKDWSEDDRPREKMLLKGANSLSNSELMAILINNGTKDKTA
                     VELAKDLLQSVDNDLRKLSKLTIQDILKLKIKGLGPAKAISIVAALELGIRRDAANKE
                     TQSYSSSNQIALYLKAKFQYLNKEVFAVVYLNNSNKIIKYEIVSEGGITGTVVDNRII
                     LKNALACSATNIVICHNHPSGSLTPSNADKKITDKIKQAANLLDIQVLDHIIVSDEGY
                     YSFADEGML"
     gene            complement(1895..>2153)
                     /locus_tag="PLU36_10595"
     CDS             complement(1895..>2153)
                     /locus_tag="PLU36_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HMZ47243.1"
                     /translation="YEPIWAIGTGKTASNEQAQEMHQHIRNVLANKYGKEIAEQISIL
                     YGGSVKGSNAADLFAQNDVDGGLVGGASLNAEEFTTIINAL"
BASE COUNT          740 a          323 c          352 g          738 t
ORIGIN      
        1 aggcggtaaa gtaaccaatt taaatggtga tttttatagc ccttatcaac caggtataat
       61 tgctacaaat ggcaaaatac acgatgaaat ggtgagaatt gtaaacggag gttctttata
      121 atttaatcaa gttcaaaaac gcatcccaaa cacatcatga atagctttgg aaaaattttt
      181 aaagtgcaaa tatttggcga aagtcatggt aattgcgttg gtgttgtaat tgatggcgta
      241 acggctggat tgccattaaa tgaagaagat tttttatcag atttagttag aaggcaagga
      301 ggaatacaaa aaggtacaac ccctagaaaa gaaatagatg tacctatttt taaaagcgga
      361 atttttaaag ataaagctac tggtacacca cttacaattt tatttgaaaa taataatatt
      421 caaagtgaag tttacgataa acaaagatct ataccaagac caggacatgt agattttact
      481 gctaataaaa aatttggtgg ttatgaagat tatagaggag gaggacattt tagtgccaga
      541 cttacaacag gcttagttgc tgctggagtt gtggctaaaa aaatgatgcc tgaaattaac
      601 attaatgctg ttgttgtaga aattgcagga gaaagggatt tagaaaaagg aattcaaaaa
      661 gcaatcaacg aaaaagatac agttggtggt attattgaat gtaaagtttt aggcttacct
      721 attggtttag gagaacctta ttttgactca ttagaatctt tattagcaca tattttattc
      781 tctattcctg caataaaagg agtagagttt ggaagtggat ttaatgctgc taaaatgttt
      841 ggaagtgagc ataacgatgc tatagaaaat aaagaaggta aaacatcttc aaattttgca
      901 ggaggcattg ttggaggcct aagtaatggt aatgaacttg tatttaggat agctgtaaaa
      961 cccgcatctt ctacacctaa agaacaagaa agtttaaatt gggaaacaaa tacaatggaa
     1021 aaattttcta tacaaggtcg tcatgattta tgtgtagcat taagggcacc agttattgtt
     1081 gaagcagtaa cagcaatcgt attagcagat tgtatgttac ttgaacaaaa aatacaaagg
     1141 gttttataac ataccttcgt cggcaaaact ataataacct tcatcgctta caatgatatg
     1201 gtctaaaact tgaatatcta ataggtttgc agcttgtttt attttatcgg ttattttttt
     1261 atctgcatta cttggtgtta aacttccact tggatggtta tgacaaataa caatattagt
     1321 tgcactacaa gccaatgcat tttttaaaat tattctatta tctaccactg ttccagtaat
     1381 tcctccttcg cttacaattt catatttaat tattttgttg ctattgttta aatatactac
     1441 agcaaaaact tctttattta aatattgaaa ttttgctttt aagtacaatg caatttggtt
     1501 agaggaagaa taagattgtg tttctttgtt tgcagcatct cttctaatac ccaattctaa
     1561 agctgctaca atactaatag cttttgcagg ccctaatcct tttattttta gttttaaaat
     1621 atcctgaata gttagtttac ttaattttct taaatcatta tctacacttt gtaataaatc
     1681 tttagcaagt tctacagcag ttttatcttt agtgccatta ttaataagta tagccattaa
     1741 ttcagaattg cttaaagagt ttgctccttt aagcaacatt ttttctctag gtctatcatc
     1801 ttcactccaa tcttttatac cagccatata aaattatttg atgaagaaat aaaacctata
     1861 agcataaaaa aactcatagg tttttgtaaa gaaattataa agcattaatt attgttgtaa
     1921 actcttctgc atttaaagat gcaccaccaa ccaaaccacc atccacatca ttttgtgcaa
     1981 ataaatctgc agcattgcta ccttttacac taccaccata taaaatagaa atttgttctg
     2041 caatttcttt tccatattta ttggctaaaa cattacgaat atgttggtgc atttcttgag
     2101 cttgttcatt acttgccgtt ttccctgtac ctattgccca tattggctca taa
//
LOCUS       DAOQPK010000313         2146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_45656_length_2146_cov_4.421808, whole genome shotgun sequence.
ACCESSION   DAOQPK010000313 DAOQPK010000000
VERSION     DAOQPK010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45656_length_2146_cov_4.421808"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..667)
                     /locus_tag="PLU36_10600"
     CDS             complement(77..667)
                     /locus_tag="PLU36_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47244.1"
                     /translation="MEKELTPTESLAIIENMINKAKSNYSDDSTLYLLWGWVIFICSI
                     GHFILLQTNIIKYPEYIWVLTWGAVIYQIYYILKKEKKKKVKSYTDEIVGYVWMGFGF
                     TMFFISIIIGNQNNWITLYPIILFMYGTPTFISGVAIRFLPLKIGGIVCWGLAFIAKF
                     TADIYVLLLLALAVLAAWIVPGYLLKAKYKNNSYEE"
     gene            complement(699..1331)
                     /locus_tag="PLU36_10605"
     CDS             complement(699..1331)
                     /locus_tag="PLU36_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47245.1"
                     /translation="MKKIILCLTLFISTSIIAQSDKFNAAMSSSIKEYEAAKTAEENI
                     AIAQKFIRIAEAEKTEWLPYYYAATIKAKMSIMGIGGDKDEVADEAQSLIDKAEALSK
                     NNCEILVIKSMIATAKMIVDPQNRYMEFGTLINKYLAEAKKADANNPRPYILESSNLV
                     NTPEQYGGGCNAAKPIAQKAMELFNSFKPASEIHPNWGKEMVEQILKNCK"
     gene            complement(1359..>2146)
                     /locus_tag="PLU36_10610"
     CDS             complement(1359..>2146)
                     /locus_tag="PLU36_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ47246.1"
                     /translation="KLGNKGQMSVAYGTFFQKPELDQIMQYNNTVINTGFNKATHYLI
                     NYIKNTSLQTFRVEAFYKKYNNLIKTFPDTTVNGNGYAKGFEIFWRDKKTIKFIDYWI
                     SYSYLDTKRDFLNYPSTLQPSFATPHTLSIVTKTFILKWKTGFNLTYNFATGRPYYNI
                     VYNNNLSKYEILDEGKTIDYHNLGFSLNYLPNLGKKNAKTFIVLVASVTNVLNSNQIY
                     SYRYSYNNLNKVAVTPTAKQFYFIGCFLSWGVDKTQDAINNNL"
BASE COUNT          687 a          338 c          294 g          827 t
ORIGIN      
        1 ttcatgataa cttgcttttg cattttgtat catggatgtt attaaatcta aactttcttt
       61 ttctgttaat ggtttattac tcttcataac tattgttttt atatttagct tttaataaat
      121 atcctggaac aatccatgct gccaaaacag ctaatgccaa tagtagtaaa acataaatat
      181 ctgcagtaaa ctttgctata aaagccaaac cccagcaaac aattccacct atttttaatg
      241 gtaaaaacct tatggcaaca ccacttataa aagtaggtgt gccatacata aataatatta
      301 tgggatataa agtaatccaa ttattctgat taccaattat tatagaaata aagaacatgg
      361 taaatccaaa ccccatccaa acatagccta caatttcatc ggtataagat ttaacttttt
      421 tcttcttttc tttttttagg atgtaataga tttggtaaat aacagctccc caggttaaaa
      481 cccaaatata ttctggatac ttaattatat tagtttgcaa aagtataaaa tggccaattg
      541 agcatataaa aataacccaa ccccataaaa ggtataaagt gctatcatcg ctataattgc
      601 ttttggcttt gttaatcatg ttttcaatta tagccaaact ttctgtagga gttaattctt
      661 tttccattgt aacactttta ataaatttgg taaatatttt acttgcaatt ttttaaaatt
      721 tgttcaacca tttcttttcc ccaatttgga tgaatttcag atgcaggttt aaaactatta
      781 aacaactcca ttgctttttg tgcaataggt ttggcagcat tacaaccacc accatactgt
      841 tctggtgtgt taactaagtt actcgattct aaaatatacg gacgaggatt attggcatct
      901 gctttttttg cttctgctaa atatttgttt attaatgtgc caaattccat atacctgttt
      961 tgtggatcta caatcatttt agcagttgca atcatacttt taattactaa aatttcacaa
     1021 ttgtttttag ataatgcctc tgctttatca attaaagatt gagcctcgtc tgcaacttca
     1081 tctttatctc caccaattcc cataatactc attttagctt ttatggtagc tgcataataa
     1141 taaggtagcc attctgtttt ctcagcttcg gcaatacgaa taaatttttg agcaatagca
     1201 atgttttctt ctgctgtttt agctgcctca tactctttaa tgctactgct cattgctgca
     1261 ttaaatttgt cggattgggc aataatactt gtactaataa atagtgttaa gcataagatg
     1321 atttttttca tgatgtgtgt tttagttgtt tgtaaatttt ataaattatt atttatagca
     1381 tcttgtgttt tatcaactcc ccaacttaaa aagcagccaa taaaataaaa ctgcttggct
     1441 gtaggggtaa cagcaacttt atttaaatta ttataagagt aacggtaact ataaatttgg
     1501 ttagagttta aaacattggt aacactcgca accaatacaa taaatgtttt agcatttttt
     1561 ttacctaagt tgggtaaata gtttaaacta aaacctaggt tatgataatc tattgttttg
     1621 ccttcgtcta aaatttcgta tttacttaaa ttgttattat aaacaatatt gtagtaaggc
     1681 ctgcccgttg caaaattata tgttaagtta aagccagttt tccattttaa aataaatgtt
     1741 ttggttacaa tgcttagggt atgtggtgtt gcaaagcttg gttgcaatgt tgatggatag
     1801 tttaaaaaat ctcttttagt atctaaataa ctataactaa tccaataatc aataaattta
     1861 attgtttttt tatctctcca aaatatttca aaacctttgg cataaccatt tccattaact
     1921 gttgtgtcgg gaaacgtttt aattaagtta ttatattttt tatagaaagc ctccactcta
     1981 aaagtttgaa gtgaagtgtt tttaatatag ttgattaaat aatgggtagc tttattaaaa
     2041 cctgtattaa ttacagtatt gttgtattgc attatttggt ccaattctgg tttctgaaaa
     2101 aaagtaccat aagctacact catttgtccc ttattgccta gtttgt
//
LOCUS       DAOQPK010000314         2132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_46239_length_2132_cov_2.684160, whole genome shotgun sequence.
ACCESSION   DAOQPK010000314 DAOQPK010000000
VERSION     DAOQPK010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46239_length_2132_cov_2.684160"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PLU36_10615"
     CDS             <1..332
                     /locus_tag="PLU36_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ47247.1"
                     /translation="NLCVDYFRKVKRTPTIKTSDDRDIFETINVVEESSETKLIANQS
                     HDRVRKMIDMLPDEQREVLVLRHFADLSFKEIADITNCSINTALGRMRYGLINLRKLM
                     LEKQIAL"
     gene            complement(417..>2132)
                     /locus_tag="PLU36_10620"
     CDS             complement(417..>2132)
                     /locus_tag="PLU36_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47248.1"
                     /translation="IVDDILVERLRKFYSQKVNLNYQPITKKDIAFRSKRITAYLNLV
                     HKMVEAKVESMKQECFEKKGELVKYFELLPEHSALKKMYIKFIAEENSLTKQKLEQEL
                     KQNIVQGAIDVNIMSKVDKLNLNKEGEVINSDALESFKGFADSTLNAAVVLSAGMNPK
                     LYNYIENFSDFFPDENNQLRKRIILKVSDFRSALIQAKFLAKKGLWVSEFRVESGLNC
                     GGHAFATDGLLTGPILEEFKQNRNAMLNELFEMYQKALETKNINCNVCPTQKITYQGG
                     IGNHEEDIFLQQFYQLDGTGWGSPFLLVPEATTVDEATLNNLAKATKDDFYISGSSPL
                     GVPFNNFKLSSSEKQRIERIEKGRPGSPCIKKLLVSNTEFTKEPICTASRQYQNLKIK
                     QLQEQGLNQEEYQEQFNIITEKACLCEGLATAAYINNDIVEKNESTAVAICPGPNTAY
                     FNKIYSLNDMVKHIYGKINLLDGVKRSNMYINELHLYIEYIKKDINHSIKNLNDKKIK
                     YYNKFKEQLENGITYYKNIIPQIFNQSEAYKKQMLTELDEAVNKINQIFSILSNTEEK
                     QTKVA"
BASE COUNT          689 a          326 c          293 g          824 t
ORIGIN      
        1 ataacttatg tgtagattac tttagaaaag taaaaagaac acctactatt aaaaccagcg
       61 atgatagaga tatttttgaa acaattaatg ttgtagaaga aagctctgaa acaaaattaa
      121 ttgccaacca aagtcatgat agagttcgta aaatgataga tatgctacct gatgaacaaa
      181 gagaggtttt agttttacgc cattttgcag acttaagctt taaagaaatt gcagatatta
      241 ccaattgcag tattaataca gcattaggta gaatgcgtta tggcttaatt aatttaagaa
      301 aactaatgtt agaaaagcaa attgctttat aaataataat agtaatcatt ataaaaaagt
      361 ttacagtttt atttggctgt aaactttttt tatggctata gtaatgctaa aaggcattaa
      421 gcaaccttag tttgcttttc ctcagtattg ctaagaatag aaaaaatttg attgatttta
      481 tttacagctt catctaattc ggttaacatt tgctttttat aagcttctga ctgattaaat
      541 atttgaggaa tgatattctt ataataggta ataccatttt caagttgctc tttaaactta
      601 ttataatatt taattttttt gtcgtttaaa tttttgatgc tatggttgat atctttttta
      661 atgtactcta tatataaatg caattcatta atgtacatat ttgatctctt aacaccatct
      721 aataaattta ttttaccata aatatgtttt accatatcgt ttaaactgta aattttatta
      781 aagtaagctg tattggggcc agggcaaatt gctactgcag tgctttcatt tttctccaca
      841 atatcgttat taatataagc tgcagttgcc aaaccttcgc ataggcaagc tttttcggta
      901 attatattaa attgttcttg gtattcttct tggtttaaac cttgctcttg taattgctta
      961 atttttaaat tttgatattg cctggatgct gtacaaattg gctctttagt aaactcggta
     1021 ttagatacta aaagtttttt aatgcatggg ctacctggtc ttcctttttc aattctttca
     1081 attctttgtt tctctgaact actgagttta aaattattaa aaggcactcc taaaggcgat
     1141 gaaccactaa tatagaaatc atctttagtt gctttggcta aattatttaa tgtagcctca
     1201 tccacagtag ttgcttctgg tactaataag aatggactgc cccaaccagt gccatctaat
     1261 tgataaaatt gttgtaaaaa aatatcttct tcatgattgc ctatgccacc ttgataagta
     1321 attttttgtg tagggcaaac gttacaatta atatttttgg tttctaatgc tttttggtac
     1381 atctcaaaaa gttcgttaag cattgcattt ctattttgct taaactcttc taaaattggt
     1441 cctgttaata atccatctgt agcaaaagca tggccaccac agtttaaacc agattctact
     1501 ctaaattcag aaacccataa tcctttttta gcaagaaatt tagcctgaat taatgcagaa
     1561 cgaaaatcac ttactttaag aataattctt ttgcgaagtt gattattttc atcagggaaa
     1621 aagtcagaaa aattttctat ataattatag agtttagggt tcatacctgc agataaaact
     1681 actgcagcat ttaaagtgct atctgcaaag cctttgaagg attctaacgc atcgctattg
     1741 ataacttcac cttctttatt caaattaagt ttatctactt tagacattat atttacgtct
     1801 atagcacctt gtacaatatt ttgtttaagc tcctgctcta atttttgttt tgttaagcta
     1861 ttttcttctg caataaattt tatatacatt ttttttaaag cagaatgctc tggtaaaagc
     1921 tcaaaatatt ttactaattc tccttttttc tcaaaacatt cctgcttcat actttctact
     1981 ttagcttcaa ccattttatg tactaaattt aagtaagcag ttattctttt agaacgaaat
     2041 gcaatatctt ttttagtgat tggttggtag tttaaattaa ccttttgaga ataaaatttt
     2101 ctcaatcttt ccaccaaaat atcatctaca at
//
LOCUS       DAOQPK010000315         2130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_46298_length_2130_cov_4.772048, whole genome shotgun sequence.
ACCESSION   DAOQPK010000315 DAOQPK010000000
VERSION     DAOQPK010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46298_length_2130_cov_4.772048"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /locus_tag="PLU36_10625"
     CDS             complement(<1..1269)
                     /locus_tag="PLU36_10625"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMZ47249.1"
                     /translation="MYQHNNDKDNLEQFLQTVADKNKMYSDEKVWNAIYKELHGYKRW
                     NALTIISFIVITVLTVFTILFNNTVKLNFIASNTFKEVKNKVPKKATTALISENIIPQ
                     QKIFSQSNKSYNTYPTLAFIEKESLPEAVHSKTNKSISLAFLDKLFLNNYPNLNGYIA
                     TTNSIEEPTINSIYNLKHSTLNINANTSLLAALNTVPKQEEQIILPKVIIQENPTTEI
                     EKYISKTAFTKNKKSAIELQVYATPSMSYRKLVDDKTRNTISLRDGNTTTPLPSVYYV
                     DVNEVVHHKPALGLEAGVGILYKLTDKVKLRTGLQFNIRKYYIDSYKSGLNIAQIAVI
                     TNNGLDTINQFATSSTTGPLNKSLKNSLYQISIPLGIQWSFLQSKNFEIGLGLSIQPT
                     LTLNKNVYLISTDYKYYTDGTSFFRKWNINT"
     gene            complement(1283..1858)
                     /locus_tag="PLU36_10630"
     CDS             complement(1283..1858)
                     /locus_tag="PLU36_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HMZ47250.1"
                     /translation="MIDQSLIQGCIDNDATAQRALYNTFSPKMMSICYRFAYNKNDAQ
                     DILQEAFLKIFSQIHTYQFKGSFEGWVRRIVVNSCINYIKRNKKFNDNVDIANAYNIQ
                     IKENNIGQMMFTKQVVECIRLLPIGFRTVLNLYAIEGYSHKEISEILDIQESTSRSQY
                     TRAKVILENILIKKGLIKNKEEELNWIAELK"
BASE COUNT          672 a          288 c          301 g          869 t
ORIGIN      
        1 agtattaata ttccattttc taaaaaatga agtaccatct gtataatatt tataatcggt
       61 tgaaattaaa taaacatttt tatttaaagt taaagttggc tgaatactaa ggcctaaacc
      121 aatttcgaaa tttttagatt gtaaaaaact ccattgaata ccaagtggaa ttgaaatctg
      181 gtataaacta ttttttaaag atttatttaa aggccctgtg gtactagaag tggcaaattg
      241 attgatggta tctaaaccat tattggtaat tactgcaatt tgagcaatgt ttaatccact
      301 tttataacta tcaatgtaat atttacgaat attaaattgt agtccagttc ttaacttaac
      361 tttatcagtt aatttatata aaatacccac accagcttct aaacctaatg caggtttatg
      421 gtgtaccact tcattaacat ctacataata aacagaaggt aggggagttg ttgtattacc
      481 atctcttaaa ctaatggtat ttctagtttt gtcatcaact agttttctgt aactcatgct
      541 aggggttgca taaacttgta actctatagc agatttttta ttttttgtga aagctgtttt
      601 agaaatgtat ttttctattt ccgttgttgg attttcttga ataataactt taggtaatat
      661 tatttgttcc tcttgtttag gtactgtgtt taaagctgct aataagctag tgtttgcatt
      721 aatatttaag gtagaatgtt ttaaattata aatactatta atggtaggct cttctattga
      781 gtttgtagtg gcgatatagc catttaaatt ggggtaatta ttcaaaaaca acttatctaa
      841 aaaagctaag ctaatactct tgtttgtttt tgaatgaacg gcttctggta gcgattcttt
      901 ttcaataaat gctaaagttg gatatgtatt gtatgattta ttagattggc taaaaatttt
      961 ttgctgcgga ataatatttt ctgaaattaa tgctgtagtt gcttttttgg ggactttatt
     1021 ttttacttct ttgaaagtgt tactggctat aaaattaagt ttaaccgtat tattaaataa
     1081 aatggtaaaa actgttaaaa cagtaataac aataaaagaa ataattgtaa gggcattcca
     1141 ccttttataa ccatgtagtt ctttgtaaat tgcattccaa actttttcat cagaatacat
     1201 tttattttta tcagcaactg tttgtaaaaa ttgctctaag ttatctttat cgttattatg
     1261 ttggtacact ttaaacttac atttatttta gttctgcaat ccagtttagt tcttcttctt
     1321 tgtttttgat taaccctttt ttaattaaaa tattttctaa aattactttt gctctggtat
     1381 attggcttct gctagtactt tcttgaatat ctaaaatttc tgaaatttcc ttgtggctat
     1441 acccttcaat ggcgtataaa tttaatactg ttctaaaacc aattggcaat aatcttatac
     1501 attcaactac ttgttttgta aacatcattt gcccaatatt gttttcttta atttgtatat
     1561 tataagcatt ggctatatcc acattatcat taaatttttt atttcttttt atataattaa
     1621 tgcaactgtt tacaacaatt cttcttaccc atccttcaaa agagccttta aattggtagg
     1681 tgtgaatttg agaaaagatt tttaaaaaag cttcttgtaa aatatcttga gcatcgtttt
     1741 tattataggc aaaacggtaa caaatactca tcatttttgg gctgaatgta ttgtacaatg
     1801 ctctttgtgc agtagcatca ttatctatac aaccttgtat taaactttgg tcaatcattt
     1861 tgagtactga ttatttaacc ctaaatgtat gacagttttt ttagaaatct gctgctttta
     1921 tacttaattg tataggcggt atttaaaagt aaaggaaaat atatttatct aaacccttct
     1981 tgctttttgt tatgcctttt ccttttggga ttataaattt tttcggcagt aaataatagc
     2041 cttaaaaact cttgtttagc atattcattg ggtaatacag ttggtagagc aatacgataa
     2101 atagtttcaa aatcgtaccc atttgcatat
//
LOCUS       DAOQPK010000316         2130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_46299_length_2130_cov_4.423133, whole genome shotgun sequence.
ACCESSION   DAOQPK010000316 DAOQPK010000000
VERSION     DAOQPK010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46299_length_2130_cov_4.423133"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PLU36_10635"
     CDS             complement(<1..373)
                     /locus_tag="PLU36_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HMZ47251.1"
                     /translation="MSDQTYSFFQSVEKSFDKAAKFTKWDQGLLEQIKACNSVYRMRF
                     PVKMDDGRIEVIEAYRVQHSQHKSPCKGGIRFSDEVNQDEVMALAALMTYKCAIVNVP
                     FGGGKGGIKINPHKYSAYELEK"
     gene            complement(423..641)
                     /locus_tag="PLU36_10640"
     CDS             complement(423..641)
                     /locus_tag="PLU36_10640"
                     /inference="COORDINATES: protein motif:HMM:NF040113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HMZ47252.1"
                     /translation="MGRIKLIASLCIILFSCYVVNAQELITEESRKAARNEQSIYIVM
                     AVVLTLMLGIFLYLFNLDKKLSKLEKEG"
     gene            complement(634..1323)
                     /gene="ccsA"
                     /locus_tag="PLU36_10645"
     CDS             complement(634..1323)
                     /gene="ccsA"
                     /locus_tag="PLU36_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HMZ47253.1"
                     /translation="MIRKSWWKLFAFILLMYTCSMGFLVKIPQQLPGVPLFHSIRNVF
                     FHVPMWFAMMIFFTVSLIYAIKYLIKPSTKHDIYSAIYAKTGVLFGVLGIITGAIWAN
                     FQWGAYWSGDPKQNGAAVALLIYFAYFILRGSLTDEEKKARVGAVYNIFAFFMLFPTL
                     WILPRMVESLHPGGQGGSGGNPALNGGDMSAEMRMVFWPAVIGFTLLGVWMSTLYIRF
                     QILKDKKLNYG"
     gene            1510..>2130
                     /locus_tag="PLU36_10650"
     CDS             1510..>2130
                     /locus_tag="PLU36_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47254.1"
                     /translation="MKYWLLFFFSVTTFFVTNAQKVFEFNSTCQQAYQEITQLKLNNG
                     QVLINKAKQQNPDNLIPLVLESYIDFFTLFFNENPNDFKIRYPNFEKRISALEEGPKS
                     SPFYDFCLSTVYLHKSAVNIKFNNKTRAGFDFQAAYKLIKQNLKAYPTFTPNHLLFGS
                     LQAVAGTIPKGYQWIAGLFGIKGSITKGLKTVNHFVNNPYDPWSKLM"
BASE COUNT          752 a          383 c          263 g          732 t
ORIGIN      
        1 ttttttctaa ctcgtatgca ctgtatttat gtgggttaat tttaatacca cctttacctc
       61 caccaaatgg tacatttaca attgcacatt tgtaggtcat taaggcagct agagccatta
      121 cttcatcttg gtttacttca tcgctaaatc ttataccacc tttacatgga cttttgtgct
      181 ggctatgctg cactctatag gcttcaatta cttcaattct gccatcatcc attttcactg
      241 gaaaacgcat tctgtaaaca ctgttacaag ctttaatttg ttctaataat ccttggtccc
      301 acttagtaaa ttttgccgct ttatcaaagc ttttttctac gctttgaaaa aaactgtaag
      361 tttgatctga cataagtttt taatttttaa ttaagcaatc attattattt ataaataaag
      421 ttttatccct ctttttctag tttagataac tttttatcca aattaaataa atacaaaaat
      481 attccgagca ttaaagttaa aacaactgcc attacaatat atatgctttg ttcatttctt
      541 gctgcttttc tactttcttc tgtaatcaat tcttgtgcat taactacata acaagaaaac
      601 aaaattatac ataaactggc tattaatttt attctaccca taattcaatt tcttatcttt
      661 taatatttga aacctaatat ataaagtact catccaaaca cctaacaaag taaacccaat
      721 aactgctggc caaaaaacca ttctcatttc tgcactcata tcgcctccat ttagagcagg
      781 attacctcct gagcctcctt gcccaccagg atgtaaactt tctaccattc ttggtaatat
      841 ccaaagtgtt ggaaacaaca taaaaaatgc aaaaatatta tacacagcgc caacccttgc
      901 tttcttttct tcatcagtta aacttcctct taatataaaa taagcaaagt aaattaataa
      961 ggcaacagca gctccgtttt gtttggggtc tccgctccaa tatgctcccc attgaaagtt
     1021 agcccaaatt gctccagtta taattcctaa aacaccaaat agaactcctg tttttgcata
     1081 gatagctgaa taaatatcgt gtttagtaga aggtttaatt aaatatttga tagcataaat
     1141 taatgatact gtaaaaaata tcatcattgc aaaccacatt ggaacatgaa agaaaacatt
     1201 tctaatagaa tgaaataatg gaacaccagg taattgttga ggaattttca caagaaagcc
     1261 catactgcat gtgtacatta gtaaaataaa tgcaaatagt ttccaccaag actttctaat
     1321 cataactgat gataccaaat aatggtttgc aaatgtagga taaagcccaa cactaaaaaa
     1381 agtaaaattt aacttaattg ctcaatagtt actaactctt ctatatttca ataaaaaata
     1441 ttccttataa aattgctaag aaaagtcaaa aaattacttt attaaagttg catcaaatac
     1501 atttgaggca tgaaatattg gttactattt ttttttagtg ttacaacttt ttttgttaca
     1561 aatgcccaaa aagtatttga atttaatagc acttgccaac aggcttatca ggaaattaca
     1621 caactgaaat taaacaatgg acaagtatta attaataaag ccaaacaaca aaatccagat
     1681 aatcttatac ctttagtttt agaaagctat attgattttt ttactctatt ttttaatgaa
     1741 aatccaaacg attttaaaat aagatatccc aattttgaaa aaagaattag tgccttggaa
     1801 gaagggccta aaagctctcc tttttatgac ttttgtttaa gtactgttta tttacacaaa
     1861 tcggctgtta atattaaatt taataataaa acaagagcag gttttgattt tcaggcagct
     1921 tataagctaa ttaaacaaaa tcttaaagcc tatcctactt ttaccccaaa ccatttatta
     1981 tttggcagct tacaagctgt tgctggtact attccaaaag gttaccaatg gattgcaggt
     2041 ttatttggaa ttaaaggtag tattacaaaa ggactaaaaa cggttaacca ttttgtaaac
     2101 aacccttacg acccatggtc taaattaatg
//
LOCUS       DAOQPK010000317         2120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_46731_length_2120_cov_3.283293, whole genome shotgun sequence.
ACCESSION   DAOQPK010000317 DAOQPK010000000
VERSION     DAOQPK010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46731_length_2120_cov_3.283293"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PLU36_10655"
     CDS             <1..617
                     /locus_tag="PLU36_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMZ47255.1"
                     /translation="NELRSIVANTNIFILSDEVYEHLIFDDLKNLSILRYQDLLERSF
                     VCFSFGKTYHCTGWKMGYCISSAALMQEFRKVHQFNCFSSNSPLQFALASYLNNQDAY
                     NLLGKQFQQKRDFFRNLMQQTPFKFIPSQGSYFECYSYEGLSNLSDKDFAIQLTKKLG
                     VTVIPVSAFYHNEEDNKVIRFCFAKQEATLQLAANRLMQFENLF"
     gene            complement(614..1744)
                     /gene="bshA"
                     /locus_tag="PLU36_10660"
     CDS             complement(614..1744)
                     /gene="bshA"
                     /locus_tag="PLU36_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HMZ47256.1"
                     /translation="MRIGIVCYPTFGGSGVLATELGKALADKNHQVHFITYQQPVRLN
                     IFNANIFYHEVQVPTYPLFDYPPYEIALSSTMVDVIIKHDLDLLHVHYAIPHASAAYM
                     AKQIVLKKTGRNIPVITTLHGTDITLVGRDKTYEPVVTFSINESDAITAVSENLKEET
                     LKHFEINKPIEVITNFVDIERFGKKPIDAFKKVIAPNAEKIFLHASNFRKVKRVEDVI
                     IIFDEVRKHLPAKLLMVGDGPERPAAEELVRTLNLEPDVRFVGKQEQMEEILAVSDVF
                     VLPSEYESFGLAALEAMAAKAIVLSTNAGGLPEINIHCKTGFMADVGDVKTLANFAIT
                     ILQDEAKLNKMKMAAYQQACKFDLNSIIPLYEKLYSRFCRMN"
     gene            complement(1808..>2120)
                     /locus_tag="PLU36_10665"
     CDS             complement(1808..>2120)
                     /locus_tag="PLU36_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HMZ47257.1"
                     /translation="LLEFKNAKGAWSKFFDVFGKNPLFIFVLSGFLPRLLTLFRINDH
                     TDENGNSVYLSAFGWFYEHICKNISTDLRVGSLVYAISMIVMYWFIVYLLDKKKIYIK
                     V"
BASE COUNT          702 a          345 c          311 g          762 t
ORIGIN      
        1 ttaatgaatt aagaagtatt gttgccaata ccaatatttt tattttgagc gatgaagtgt
       61 atgagcattt aatttttgat gacctaaaaa atttaagtat tcttcgttat caagatttat
      121 tagaaagaag ttttgtttgc ttttcttttg gcaaaaccta tcattgtact ggctggaaaa
      181 tgggttattg tattagtagt gctgctctaa tgcaagagtt tagaaaagta catcagttta
      241 attgtttcag cagcaacagc cccttacaat ttgcattagc aagttactta aataatcaag
      301 atgcatataa tttactaggc aaacaatttc aacaaaaaag agattttttt agaaacttaa
      361 tgcagcaaac accctttaag tttataccct ctcaaggtag ttattttgag tgttacagtt
      421 atgaaggatt aagtaattta agcgataaag attttgctat acagctaaca aaaaagttag
      481 gggtaacagt aatacccgtt tctgcatttt atcataacga agaagataat aaagtaattc
      541 gtttttgttt tgcaaagcaa gaagctactt tacaacttgc agcaaataga ttaatgcagt
      601 ttgaaaattt attttagttc attctgcaaa acctactata caatttttca tataatggta
      661 taatactatt caaatcaaat ttacaagctt gttgataagc tgccattttc attttattta
      721 atttggcttc gtcttgtagt attgttattg caaaatttgc caaagttttt acatctccaa
      781 catctgccat aaaacctgtt ttgcagtgta tatttatttc tggcaaacct cctgcattag
      841 tgcttaatac aatagctttt gcagccattg cttctaatgc agctaagcca aaactttcat
      901 attcgctggg tagtacaaaa acatcactta ctgcaagtat ttcttccatt tgttcttgct
      961 tgcctacaaa ccttacatcg ggttctaaat ttaaagttct aactaattct tctgctgcag
     1021 gtctttctgg tccatcgcca accattagta atttggcagg taaatgtttt ctcacttcat
     1081 caaatattat aattacatct tctactcttt taacttttct aaaattacta gcatgtaaaa
     1141 atattttttc ggcatttggt gctattactt ttttaaaagc atcaataggc tttttgccaa
     1201 atctttcaat atctacaaag ttggttatta cctcaatagg tttatttatt tcgaagtgtt
     1261 ttaaggtttc ttcttttaag ttttcgctta cagcggtaat agcatcactt tcgttaatag
     1321 aaaatgttac aacaggttcg taggttttat ctctgccaac taaggttata tctgttccat
     1381 gtaatgtagt aattacagga atatttcttc cggttttctt taatacaatt tgcttggcca
     1441 tatatgcagc acttgcatgt ggtatggcat aatgaacgtg cagtaaatct aaatcgtgtt
     1501 taataattac atctaccatt gtactagata gggcaatttc gtatggtgga taatcaaaca
     1561 aaggataagt aggaacttgt acttcgtggt aaaaaatatt agcattaaaa atgtttaacc
     1621 taaccggctg ctggtaagta ataaaatgta cctgatggtt tttatctgct aaagctttgc
     1681 ctaattcagt tgccaaaact ccgcttccgc caaaagttgg ataacaaaca attcctatac
     1741 gcatttttta ttttttatac tttaaagatg atgaaggtaa gcttatactt tcaaggaaga
     1801 aaagggttta tacttttata taaattttct tcttatctaa taaataaaca ataaaccaat
     1861 acataacaat catactaatt gcataaacta atgaacctac tcttaaatcg gttgaaatat
     1921 ttttgcaaat atgttcataa aaccaaccaa aggcagatag ataaaccgag ttgccatttt
     1981 catcagtatg atcattaatt ctaaacaggg taagcaaacg tggtaaaaag ccactcagca
     2041 caaatatgaa caaaggattt tttccaaata catcaaaaaa tttactccat gcacctttag
     2101 catttttaaa ttcaagcaaa
//
LOCUS       DAOQPK010000318         2110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_47144_length_2110_cov_3.988808, whole genome shotgun sequence.
ACCESSION   DAOQPK010000318 DAOQPK010000000
VERSION     DAOQPK010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47144_length_2110_cov_3.988808"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /gene="rpsE"
                     /locus_tag="PLU36_10670"
     CDS             complement(<1..509)
                     /gene="rpsE"
                     /locus_tag="PLU36_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HMZ47258.1"
                     /translation="MSKVSDNKVKAGGDMELKEKVVAINRVVKTTKGGRTFSFSALVV
                     VGNENGVVGQGLGKAKEVQEAITKGIEDAKKNLVKVPIMRGTIPHEQFIKSGAAKVLI
                     KPAAQGAGVIAGGSMRAVLESAGFTDVQAKSLGSANPHNVVKATIKALTALREPVQVA
                     KNRNIKLSKV"
     gene            complement(534..887)
                     /gene="rplR"
                     /locus_tag="PLU36_10675"
     CDS             complement(534..887)
                     /gene="rplR"
                     /locus_tag="PLU36_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HMZ47259.1"
                     /translation="MSSNISNKQKIKYRIRKKISGTAVKPRLSVFRSNAEIYAQLIDD
                     NNGVTLTAAASTDKEIAAQKSTKSEKSKLVGQAIARKAIELGLSTCVFDRGGNLYHGR
                     IKAVAEGAREGGLKF"
     gene            complement(940..1494)
                     /gene="rplF"
                     /locus_tag="PLU36_10680"
     CDS             complement(940..1494)
                     /gene="rplF"
                     /locus_tag="PLU36_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017722933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HMZ47260.1"
                     /translation="MSRIGKQPISIPSGVTVSIANGNVVTVKGPKGELKESIDRDIAI
                     EVKDGQILITRPTDQIRHRALHGLYRALINNLVKGVTDGYKKELELVGVGFKAANTGN
                     TLDLALGYSHNIIFEVPKELKVATATEKGKNPIITLEGSDKQLIGQVAAKLRSLRKPE
                     PYKGKGVKFVGEVLRRKAGKSAGK"
     gene            complement(1630..2028)
                     /gene="rpsH"
                     /locus_tag="PLU36_10685"
     CDS             complement(1630..2028)
                     /gene="rpsH"
                     /locus_tag="PLU36_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HMZ47261.1"
                     /translation="MVTDPIADYLTRIRNAQMAGHRIVEIPASNLKKRITEILYEQGY
                     ILRYKFEDDSKQGIIKIALKYDASTKQPAIRNLERVSRPGLRQYAKPTEIKRVINGLG
                     VAIISTSKGVLTDKQAKVQNVGGEVLCYIS"
BASE COUNT          595 a          409 c          288 g          818 t
ORIGIN      
        1 actttactta atttaatatt tctattttta gcaacctgaa ctggctctct taatgcagtt
       61 agtgctttaa tagtagcttt taccacatta tgaggattgg cagagcctaa gctttttgct
      121 tgaacatctg taaaaccagc actttctaaa acagcacgca tgctaccacc agcaattaca
      181 ccagcaccct gagctgcagg tttaattaaa actttagctg caccagattt aataaactgt
      241 tcgtgaggta tagtaccacg catgatggga acttttacta aatttttctt agcatcttca
      301 atcccttttg taatagcttc ttgtacttct ttggcttttc ctaatccttg cccaactaca
      361 ccattttcat tacctacaac cactagtgct gagaaagaaa atgtacgacc accttttgta
      421 gtttttacta cacgattaat ggcaactact ttctctttta attccatgtc gccgccggct
      481 tttactttat tatcgcttac tttagacatt attgttaatt tgaaatttat ttattaaaat
      541 ttaaggcctc cttctcttgc tccctcagca actgctttaa tacgaccatg atataaatta
      601 ccaccacgat caaaaacaca tgtagataaa cctaattcta ttgctttacg tgcaatagct
      661 tgacctacta gtttagactt ttctgattta gtactttttt gtgcagctat ttctttatct
      721 gtacttgcag cagcagttaa tgttacgcca ttgttatcat caattaattg agcataaatt
      781 tctgcattgc ttctaaaaac acttaaacga ggctttactg ccgtaccgct aattttttta
      841 cgaatacgat acttaatttt ttgcttatta ctaatgttac tactcatgat tttatttttt
      901 acttaccgat tctgccggta atatttttat aaaattttat tatttaccag ccgatttacc
      961 agcttttctt cttaatactt cacctacaaa tttcacccct ttccctttat atggttctgg
     1021 tttacgtagg cttcttaatt tagcagctac ttgaccaata agttgtttat cggatccttc
     1081 taaagttata attgggtttt tacctttttc ggtagcagtt gcaactttta attctttagg
     1141 cacttcaaaa ataatattgt gagagtatcc taaagctaaa tctaaagtgt tgcctgtgtt
     1201 agctgcttta aaaccaaccc ctactaattc taattctttt ttataaccgt ctgttacgcc
     1261 tttaactaaa ttgttgatta aggcacgata taggccatgt aatgctctat ggcgaatttg
     1321 atcggttgga cgagtgatta atatttgacc atcttttacc tcaatagcaa tatcacggtc
     1381 aatagattct ttcaattctc cttttgggcc ttttacagtt actacgttac cgtttgcaat
     1441 gctaacagta acaccagaag gaattgaaat gggttgttta cctatacgag acataatctt
     1501 atttttattt ttttatacta caattttgat aattttagaa gtgttcagct accgtatagt
     1561 aagcttaaat tgtcttcata ttgttaatgt aaagtggtta ttatcaaacc tactttacaa
     1621 aattttatat taactaatat agcaaagtac ttctccgcct acgttttgta ctttagcttg
     1681 tttatctgtt aacacacctt ttgatgtact aataattgca acacctaagc cattaattac
     1741 tcttttaatt tcagtaggtt ttgcatattg acgtaaacca ggtcgactaa ctctttctaa
     1801 gttacgaata gcaggttgct tggttgaagc atcgtatttt aaggcaattt taattatacc
     1861 ttgtttgcta tcatcttcaa acttgtatct caaaatatat ccttgttcat acaatatttc
     1921 agtaattctt ttcttcaaat ttgaagcagg aatttctact attctatgac ccgccatttg
     1981 agcgttacga atccttgtta aataatctgc tataggatca gttaccattt gtattaaatt
     2041 aaatcagttc ttaaatattg tttcaaattg ttactctaca aagagatttt ttgttgttac
     2101 cagcttgcct
//
LOCUS       DAOQPK010000319         2091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_47944_length_2091_cov_4.389980, whole genome shotgun sequence.
ACCESSION   DAOQPK010000319 DAOQPK010000000
VERSION     DAOQPK010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47944_length_2091_cov_4.389980"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="PLU36_10690"
     CDS             <1..786
                     /locus_tag="PLU36_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMZ47262.1"
                     /translation="EGDIRDTALLEKCIIGCDYVFHMAALRINACAANQQDGFDVMLQ
                     ATFSVAEFCKKHAIKKVIYSSSASVYGLAQNFPTPETDNPYNNQTFYGAAKMWGEQLF
                     RSYKFMHGLDYVALRYFNVYGERMDTDGKYTEVMIKWLDCIRNGKQPAIFGDGSDTMD
                     FVHVKDVAHSNILALLADVTDEVFNVGVQRETSLKELLETLLKVNNCHLEPVFKEANS
                     VNPVKKRIADLNKINTLLGYKPSITLEAGLKSLSDWYFNLQQK"
     gene            793..1920
                     /locus_tag="PLU36_10695"
     CDS             793..1920
                     /locus_tag="PLU36_10695"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HMZ47263.1"
                     /translation="MIPLAKPYLDKEDAQNAYDTILTGWVTQGPRVQEFEEKFAQYVG
                     SKYAVALSNCTTALHLAMIVAGVGNDDEVICPSMSYVATANSIRYTGARVVFAEVNPL
                     TYNLDVNDVRNKITSKTKAILLVHQIGMPADIDAFKTLCTEKNLILIEDAACAAGSAY
                     KGKKIGSHSDLVCFSFHPRKVITTGDGGMITTNNEAYRDRIKLLRQHAMSVNDRVRHE
                     SDKVIFEDHIELGYNYRMTDIQAAVGIKQLEKLDFIISERRKIAFQYHEAFKEIDCIR
                     LPLEREGYFTNYQSYSIYLKPNCKISRNDLMQALLDEGISTRRGISTAHRETVYKGYQ
                     ENYSLSISEDASDRSIVIPLYYPMTGEEVDYVITNISSILK"
BASE COUNT          745 a          295 c          407 g          644 t
ORIGIN      
        1 gaaggtgata tacgtgatac tgcgttatta gaaaaatgta ttattggttg tgattatgta
       61 tttcacatgg cagccttgcg tattaatgca tgtgccgcaa atcagcaaga cggttttgat
      121 gtgatgttgc aggcaacatt tagtgttgca gaattttgta aaaaacatgc tattaaaaaa
      181 gtgatttaca gttcgagtgc gtctgtatat ggattagcac aaaattttcc aacacctgaa
      241 acagataatc catataacaa tcaaacattt tacggagcag caaaaatgtg gggcgagcaa
      301 ctgtttcgtt cctacaaatt tatgcatggt ttagattatg tggcgctccg ctactttaat
      361 gtatatggtg aacgtatgga tactgatgga aaatatacag aagtaatgat aaagtggctg
      421 gattgcattc gcaatggtaa acaaccagct atttttggtg atggttctga tacgatggat
      481 tttgtccacg tgaaagatgt agcacacagt aatattttag cattattagc agacgttaca
      541 gacgaagtat ttaatgttgg tgtgcaacga gaaacttctc tgaaagaatt attagaaaca
      601 ttattgaaag taaataattg tcatttagaa cccgtattta aagaagctaa ttcggtaaat
      661 ccagtaaaaa aaagaattgc tgatttgaat aaaataaata cattgttagg atacaaaccg
      721 agtattacac tggaagctgg tttgaaatct ttatcagatt ggtattttaa tcttcaacaa
      781 aaataaaaaa ttatgattcc tttagcaaaa ccatatttag acaaagaaga tgcacaaaat
      841 gcctacgata ctatcttaac tggttgggta acacaaggtc cacgtgtgca agagtttgaa
      901 gagaaatttg cacaatatgt aggtagcaaa tatgcagtgg cattgtcgaa ttgtacaacg
      961 gcattacatt tagccatgat tgttgctggt gttggcaatg acgatgaagt tatttgccca
     1021 tctatgagtt atgtggcaac agcaaacagc attagatata caggagcaag agtagtattt
     1081 gctgaagtaa atccgcttac ttataattta gatgtaaatg atgtaagaaa taaaattaca
     1141 tctaaaacaa aagcaatatt gcttgtacat caaattggaa tgccagcaga tattgacgca
     1201 tttaaaacat tgtgtacaga aaaaaatctg atattgattg aagatgctgc atgcgcagct
     1261 ggttctgcat ataaaggaaa aaaaattgga agccattcag atttagtatg cttttcattt
     1321 catccacgta aagtaataac aacaggcgac ggtggcatga ttactaccaa caatgaagcc
     1381 tatagagata gaataaaatt gttgcgccaa catgcaatga gtgtaaatga cagagtacgc
     1441 cacgaatctg ataaagttat ttttgaagac catatagaac ttggatataa ttacagaatg
     1501 actgatatac aagctgctgt tggtattaaa cagttggaga aattagattt tattatttca
     1561 gaacgaagaa aaatagcctt ccaatatcac gaagcattta aagagattga ttgtattcga
     1621 cttccattag agcgagaagg ctattttacc aattatcaat cgtatagtat atatttaaaa
     1681 ccaaactgca aaataagtag aaatgatttg atgcaggcat tgttagacga aggtatttca
     1741 acgagaagag gcatctctac tgcacataga gaaacagtgt ataaaggcta tcaagaaaac
     1801 tattcattat ctatttcaga agatgctagt gatagaagta ttgttattcc tttatattat
     1861 cctatgactg gagaagaggt agattatgtg attactaaca ttagctcaat tttaaagtaa
     1921 aatcatgatt tataagttat ttccgttatt gtttacaagc acattattgg aattccaaga
     1981 aaaaagtgga caatatacgt tgagcattat tcgtattgct gcttgaaatg cttgtatgct
     2041 attgttagtg taatattctt catatcgatc ataagaacta cgtttatcct g
//
LOCUS       DAOQPK010000320         2075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_48618_length_2075_cov_5.219802, whole genome shotgun sequence.
ACCESSION   DAOQPK010000320 DAOQPK010000000
VERSION     DAOQPK010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48618_length_2075_cov_5.219802"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PLU36_10700"
     CDS             complement(<1..203)
                     /locus_tag="PLU36_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HMZ47264.1"
                     /translation="MTSTVVYKGDLRTELTHLQSGKVIETDAPTDNHGKGERFSPTDL
                     MATSYASCMITTMAIKAADMNLN"
     gene            246..1064
                     /locus_tag="PLU36_10705"
     CDS             246..1064
                     /locus_tag="PLU36_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47265.1"
                     /translation="MKKNIVVLIFSFIPALLLAQQTKNLTPRQQKKADRREKIENLIK
                     KEEEGALIFNKQTVFGIKLSTDGYGLHFEKGKFKTNKLTTLWWSELNEHKHAKEQKFA
                     TGDPNWGFTIGTPYVYGKINNFYNYKLGYGQQRLIGGKGTKNGVATSLIYGGGIAIAI
                     LKPYFLDVQDPLSLETKTIRYKDDEQLFLDQTRIIGSAGIFKGIGYSKFIPGFHVKTA
                     LRFDYGRYNEMISALEAGINFEYYTKEIEHMALNKNHKMFFNAYVALVFGKRKN"
     gene            complement(1113..1718)
                     /locus_tag="PLU36_10710"
     CDS             complement(1113..1718)
                     /locus_tag="PLU36_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HMZ47266.1"
                     /translation="MISNDTKNILGLQLPTDPRWANLTEISLEAILTDHAYCEQKAAT
                     TCISLIQRFSKREKLVKELAPIVTEEWGHFRLVLNELHKRNLKLGNQRKDEYVNQLIA
                     FQQKGGNEEGRLLDQLLTMALIEARSCERFKLLSENLSDKYLRNFYRRFMESEAGHYT
                     LFITLAETYIDVLKVKKRWKEWLAYEADIMKSLELRGDRIH"
     gene            complement(1729..>2075)
                     /locus_tag="PLU36_10715"
     CDS             complement(1729..>2075)
                     /locus_tag="PLU36_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family peptidase"
                     /protein_id="HMZ47267.1"
                     /translation="NIDYALANINLDTITNKAILTLKRIGKMPMPIDVLITYKDGSSE
                     MYYIPLNLMYGNKPNESNTKNFVLTEWKWTHPEYTFSINKNINEIKSIEIDPTQRMAD
                     INRINNKILIPD"
BASE COUNT          682 a          315 c          316 g          762 t
ORIGIN      
        1 aaatttaagt tcatatcggc agcttttatt gccattgtag taatcataca acttgcatag
       61 cttgtagcca ttaaatctgt tgggctaaaa cgttctcctt ttccatgatt atcagtaggt
      121 gcatctgttt caattacttt tccactttgt aagtgtgtta attctgttct taaatctcct
      181 ttgtaaacaa ctgtacttgt cataatacta aattttgccc taaatttaca accaataatt
      241 tattcatgaa aaagaatatt gtagtactga tatttagttt tatacctgct ttattacttg
      301 cacagcaaac aaaaaacctt actccacgcc agcaaaagaa agcagataga agagagaaaa
      361 tagaaaacct aattaagaaa gaagaagaag gagcattgat ttttaataag caaactgtat
      421 ttggcattaa actaagtaca gatggttatg gtctgcattt tgaaaaagga aagtttaaaa
      481 caaataagtt aactaccctt tggtggtcgg aattaaatga gcataaacat gctaaagaac
      541 aaaaatttgc cacaggtgat cctaattggg gatttacaat tggtacacct tatgtatatg
      601 gtaaaataaa taatttttat aactataaat taggctatgg tcagcagaga ttaattggag
      661 gcaaaggcac taaaaatgga gtagcaactt cgttaattta tggaggagga atagccattg
      721 caattttaaa gccttacttt ttagatgtgc aagacccact tagtttagaa acaaaaacta
      781 taaggtataa agatgatgaa caattatttt tagatcaaac cagaataata ggctctgcag
      841 gaatttttaa aggaattggt tattcgaaat ttattccagg ctttcatgta aaaactgctt
      901 taaggtttga ttatggcaga tataacgaaa tgatatctgc tttagaagca ggtataaact
      961 ttgagtatta tactaaagaa attgaacaca tggctcttaa taaaaaccat aaaatgtttt
     1021 ttaatgctta tgtagccttg gtatttggta aaagaaagaa ctaagtacca attcttttct
     1081 gttatttatg aaaaacatgc aagcttaaaa aattaatgta ttctgtcgcc ccgtagctct
     1141 aatgatttca taatatctgc ttcgtaagca agccactcct tccaacgttt ttttactttg
     1201 agtacatcaa tgtaagtttc tgcaagtgtt ataaacaaag tataatggcc tgcttcgctt
     1261 tccataaaac gcctgtaaaa atttcttaaa tacttatcgc ttaaattttc gcttaataat
     1321 ttaaatcttt cacaacttct ggcttcaatt aaagccatag taagtaattg gtctaatagc
     1381 cttccttctt cattaccacc tttttgctga aacgcaatta attggtttac atattcatct
     1441 tttcgttgat ttcctagttt taaatttctt ttatgtagct cgttaagaac aagtctaaaa
     1501 tgtccccatt cttctgtaac tattggtgct aattctttta ctaatttttc tcttttacta
     1561 aatctttgaa ttaatgaaat acatgttgta gctgcttttt gctcgcagta agcatgatcg
     1621 gttaaaatag cttctaagct aatttctgtt aaattagccc aacgtggatc ggttggtaat
     1681 tgtaacccta atatattttt tgtatcgttt gaaatcatgt ttaatttctt aatccggaat
     1741 aagtatttta ttgtttatcc tgtttatgtc tgccattctt tgagtagggt caatttcaat
     1801 agattttatt tcattgatat ttttattaat gctaaaagta tattcaggat gtgtccactt
     1861 ccactctgtt aatacaaagt ttttggtatt actttcgttt ggtttgttgc catacataag
     1921 gtttaaaggt atataataca tttcgctact accatcttta taggttatta aaacatcaat
     1981 tggcataggc attttaccaa ttctttttaa agttaatatt gctttgttag taatagtatc
     2041 taggttaata ttagccaaag cataatctat atttt
//
LOCUS       DAOQPK010000321         2066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_49030_length_2066_cov_3.642964, whole genome shotgun sequence.
ACCESSION   DAOQPK010000321 DAOQPK010000000
VERSION     DAOQPK010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49030_length_2066_cov_3.642964"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2066)
                     /locus_tag="PLU36_10720"
     CDS             complement(<1..>2066)
                     /locus_tag="PLU36_10720"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HMZ47268.1"
                     /translation="LYVDELPLPALSTGMVLVENEFSLISAGTEKGTVKVAQSSLLGK
                     AKQRPDLVAQVLQNIKKEGLMATLEKVRTKLDSLKALGYSTAGVVKASLDTNGKFKVG
                     DRVACAGQDYASHAEVVSIPQNLVAKIPDNVSFEEASFTTLGAIALQGVRQANPTLGE
                     NICVIGLGLLGQITCQLLSANGCRVFGIDVSDKMVQLAKEVSNAEAYNRNNAALLTAI
                     ETFTRGNGFDKIIITAAAPTNDPIELSVQIARKKGQIIVVGAVKMDVPRDPHFYRKEL
                     DLKISCSYGPGRYDNVYEEQGHDYPFAYVRWTEQRNMEAFLDLIALGKLNLKALITHT
                     FDIDDAVRAYDLILGKVQEPFIGILLKYPENEKRKLSTFQTKNSNTNHTIKASFIGAG
                     SFAQSYLIPNLKGLPVSLDAVVTNNGINAKNVSSKFGFARASTNANEIIEDKNSNTIF
                     IASRHDSHAQYVIKSLQENKHVFVEKPLCITYEELEAVTEVVNQKPNQLLMVGFNRRF
                     APVSVELNKTFSKITEPKVINIRVNAGFIPLDHWTQIATIGGGRIVGEMCHFIDLMQY
                     FTNSNPVQVYAQCISSTSSQVKNDDNIAIVVTFADGSIGNLVYVANGDKAMPKERIEV
                     FAGGCIGVINDFKDGQLYKGNKQTTIKLSGKGHKQEVESFIHAVKNGTSSPIAFNSIY
                     YTTLTT"
BASE COUNT          654 a          382 c          286 g          744 t
ORIGIN      
        1 agtagttaat gtggtataat aaatagaatt aaaagctatt ggcgaagaag tgccattttt
       61 tactgcatgg ataaaagact ctacttcttg tttatgcccc ttaccagata gtttaatggt
      121 tgtttgttta ttgcctttat ataattgtcc atctttaaaa tcgttaatta caccaataca
      181 gcctcctgca aaaacttcaa ttctttcctt aggcatggct ttatctccat tagcaacata
      241 aactaaattt ccaattgagc cgtctgcaaa agttactaca atagcaatat tatcatcatt
      301 tttaacttga gaacttgtac ttgaaataca ttgtgcataa acctgcacag gatttgagtt
      361 ggtaaaatat tgcatcaaat caataaaatg acacatttct ccaacaattc tgcctcctcc
      421 aattgttgct atttgtgtcc aatggtctaa aggaataaaa cctgcattta ctcttatatt
      481 aataactttt ggttctgtta tttttgaaaa agttttattc aattctacac ttactggtgc
      541 aaatcttcta ttaaaaccaa ccattaaaag ttgatttggt ttttgattta ccacttctgt
      601 aacagcttct aactcctcat aggtaataca caaaggtttt tcaacaaaaa cgtgtttatt
      661 ttcttgcaaa gattttataa catattgtgc atgactatca tgacgagaag caatgaaaat
      721 tgtattacta tttttatctt caataatttc attagcattg gtagatgctc ttgcaaagcc
      781 aaattttgag gaaacatttt ttgcattaat accattgttg gtaacaacag catctaagct
      841 aacaggtaag ccttttaaat tgggtatcaa ataactttga gcaaaactac cagcaccaat
      901 aaaagaagcc tttatagtgt gattagtatt actattttta gtttgaaaag tacttagttt
      961 tcttttctca ttttctggat attttaataa aataccaata aagggttctt gtacttttcc
     1021 taaaattaaa tcataggctc taactgcatc atcaatatca aaagtatggg ttattaatgc
     1081 ttttaaattt aattttccta atgcaattaa atctaaaaaa gcttccatat ttctttgctc
     1141 tgtccatctt acataagcaa agggataatc atgtccttgc tcttcatata cattatcgta
     1201 cctgccaggt ccataggaac aagagatttt caaatctaac tcttttctat aaaaatgagg
     1261 gtctcttggt acatccattt taactgcacc taccacaatt atttgccctt tttttctggc
     1321 aatttgtact gataattcta tggggtcgtt tgttggagca gcggcagtaa taattatttt
     1381 atcaaatcca ttacctctgg taaaagtttc tatagcagta agtaaagctg cattatttct
     1441 attataagcc tctgcattac ttacttcttt tgctaattga accattttgt ctgatacatc
     1501 aataccaaaa actctacaac cattagcaga taaaagctga caagtaattt gccctagtaa
     1561 acccaagcca ataacacata tattttctcc taaggttggg ttagcttgcc ttactccttg
     1621 caaggcaatt gcacccaaag tagtaaaact tgcttcttca aaagaaacat tatcaggaat
     1681 ttttgctacc aagttttgag gaatagaaac tacttcggca tgagaagcat aatcttgccc
     1741 tgcacaagct actctatctc caactttaaa tttaccatta gtatccaaag aggcttttac
     1801 tacacctgct gtgctgtaac ctaaggcttt taaagaatct aatttggttc ttactttttc
     1861 aagcgttgcc attaaacctt ctttctttat attttgcaaa acctgtgcaa ctaaatcggg
     1921 gcgttgtttt gcttttccta ataaagatga ttgagctact ttaactgttc ctttttctgt
     1981 acctgcactg attaatgaaa attcattttc taccaatacc atacctgttg ataatgcagg
     2041 taaaggaagt tcatcaacat ataatt
//
LOCUS       DAOQPK010000322         2059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_49345_length_2059_cov_4.289920, whole genome shotgun sequence.
ACCESSION   DAOQPK010000322 DAOQPK010000000
VERSION     DAOQPK010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49345_length_2059_cov_4.289920"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PLU36_10725"
     CDS             complement(<1..554)
                     /locus_tag="PLU36_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMZ47269.1"
                     /translation="MHIQSKLPQIGTTIFTTMSSLATEYNAVNLGQGFPDFEMNKELI
                     DLVHAAMLQGNNQYVHSNGLALLRDRIAEKYANLYQSNIDADKQITITPGGTYAIYTA
                     LTTVLNAGDEVIIFEPAYDSYIPNIVVNGAKPICISLQYPNYAIPWNEVRAKVNSKTK
                     MIIINSPHNPTGAVLSQEDINELR"
     gene            complement(612..>2059)
                     /locus_tag="PLU36_10730"
     CDS             complement(612..>2059)
                     /locus_tag="PLU36_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HMZ47270.1"
                     /translation="FINRFKSSKEKKETLQKLQEGKIDIIIGTHGLLGKDVKFKDLGL
                     LVIDEEQKFGVAHKEKLKLLKTNVDSLTLTATPIPRTLQFSLMGARDLSIINTPPPNR
                     QPIHTEIQIFNDEFIRDTIYFETERGGQVFFIHNRVVGLPEMAAFIQAQCPDLQVAYA
                     HGQMEGSQLEEKILDFIDKKYDVLICTNIVESGVDIPNVNTIIINNAHQFGLSDLHQL
                     RGRVGRSNKKAFCYLLAPPISTLPVDSRKRLQTLEQFSDLGSGFQIAMRDLDIRGAGD
                     MLGGEQSGFIAEIGFETYQKILDEAIRELKRTSFKELFKEEISKQDDYVNDCTIDTDL
                     QILIPDFYIESITERLSIYTRMNDCKKEEELQLLYTEMIDRFGNIPTEVDDLFTTVRC
                     RWLAIDLGFEKMSLKENTMRCYFVNKNDSPYFESNTFKNIINFLQKETNKGRLKQVAK
                     NFLLVVDNINGMSEMLTFLNKMHNFVIAKNS"
BASE COUNT          631 a          362 c          289 g          777 t
ORIGIN      
        1 cttcttaatt cattaatatc ctcttggctt aaaactgcac cagttgggtt atgtggcgaa
       61 ttgataataa tcattttagt tttactattc actttagccc ttacttcgtt ccatggaatg
      121 gcgtagtttg gatattgaag agaaatgcaa ataggctttg ccccattaac tacaatattg
      181 ggtatataac tatcatacgc tggttcaaaa ataattactt catctccagc atttaaaaca
      241 gttgttagtg cagtgtaaat agcataagtg ccacccggag tgatagtaat ttgtttatct
      301 gcatcaatat tggattgata aagattggca tacttttctg caattctatc tcttaataaa
      361 gcaagcccat tactatgtac atattgattg ttgccttgca acatagcagc atgtaccaaa
      421 tcaattaact ctttattcat ctcaaaatcc ggaaaccctt gccctaaatt aacagcattg
      481 tattctgtag ctaagctgct cattgtagta aaaatagttg tacctatttg aggtaattta
      541 gattgtatgt gcatgtaacg aagataaaaa gaagcagcaa gaataaatat agcttagggg
      601 agattttttg cttaactatt tttagcaatt acaaagttgt gcattttatt taagaaggtg
      661 agcatctcac tcataccatt gatattatca actactaaca aaaaattttt agctacttgc
      721 tttaaccttc ctttattggt ttctttttgt aaaaaattaa ttatgttttt aaaggtgtta
      781 ctttcaaaat aagggctgtc atttttattt acaaagtagc aacgcatggt attttctttg
      841 aggctcattt tttcgaaacc taaatcaatt gcaagccaac ggcaacgaac agtagtaaac
      901 aaatcatcta cctctgttgg tatatttcca aatctatcta tcatttcggt gtataataat
      961 tgtaactctt cttctttttt acagtcattc attctggtat aaatagaaag tctttctgta
     1021 atgctttcta tataaaaatc tggaataaga atttgcaagt cagtatctat tgtacaatcg
     1081 tttacataat catcttgttt actaatttct tctttaaata attctttaaa cgaggttctt
     1141 ttaagttctc taattgcttc atctaaaatt ttctgatagg tttcaaaacc aatttctgca
     1201 ataaaaccac tttgttcacc accaagcata tctcctgcac cacgaatatc taaatctctc
     1261 atggcaattt gaaagccact gcctaaatcg ctaaactgct ctaaggtttg taatcttttt
     1321 ctactatcta caggcaatgt actaattggt ggggctagca aataacaaaa agctttttta
     1381 ttgcttcttc ctactcttcc tcttaattga tgcaaatcgc ttaaaccaaa ctgatgagca
     1441 ttatttataa ttatggtatt aacgtttgga atatctacac cactttctac aatattggta
     1501 caaattaata catcatattt tttatcaata aaatctaaaa ttttttcttc caactggctg
     1561 ccttccattt gcccatgtgc ataagcaact tgcaaatcgg ggcattgtgc ttgtataaaa
     1621 gctgccattt ctggcaaccc aactaccctg ttatgaataa agaaaacttg ccctcctctt
     1681 tctgtttcaa aataaatagt gtctctaata aattcgtcat taaatatttg tatttcggta
     1741 tgtatgggct gcctgtttgg tggtggtgta tttattatac ttaaatctct tgcacccatt
     1801 aaactaaatt gaagtgtacg tggtatagga gttgctgtta atgttaaact atctacattg
     1861 gttttaagta attttaattt ttctttatgt gctacaccaa atttttgttc ctcatcaatt
     1921 actaataacc ctaaatcttt aaacttaaca tctttgccta ataagccatg agtgcctatg
     1981 ataatatcta tttttccttc ctgtagtttt tgtaaggttt cttttttttc tttagatgat
     2041 ttaaatctat ttataaaat
//
LOCUS       DAOQPK010000323         2047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_49887_length_2047_cov_4.745984, whole genome shotgun sequence.
ACCESSION   DAOQPK010000323 DAOQPK010000000
VERSION     DAOQPK010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49887_length_2047_cov_4.745984"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..781)
                     /locus_tag="PLU36_10735"
     CDS             complement(14..781)
                     /locus_tag="PLU36_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47271.1"
                     /translation="MNKIFWKYFIAFGLIIIFKNTNAQKIDSLLKEAEKLEFKLKEVD
                     ALNKYKEVLTIYGDNFKALIKASELSIAIGARLPALKDKKLYFESANSFANRAFSVDS
                     SHADACYVIALVNSNYILIEKENKALGAYINTTKQFAEKAIQLNNKHAKAHFILGNWY
                     LEMVNLSGLKKTAVKLFYGTLPKYSLEDAIKNLELCRLYDQYYMINYLQLAKAYIQDN
                     NPTKAIEVLQKLKKLPLRTADDAAIKEEGVSLLSSLQ"
     gene            complement(805..1935)
                     /gene="hppD"
                     /locus_tag="PLU36_10740"
     CDS             complement(805..1935)
                     /gene="hppD"
                     /locus_tag="PLU36_10740"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HMZ47272.1"
                     /translation="METSVARKVADQTANDFLPLHGTDYVEFYVGNAKQAAHFYKTAF
                     GFQSVAYAGPETGVKDKASYVISQNKLTFVLTTPLKTNNPIADHIYKHGDGVKVLALR
                     VDDATDAWHQTTKRGGKSYLEPVTLKDNDGEVVLSGIHTYGDTVHIFVERKNYKGAFM
                     PGYVKWNSTYNPASTGLLYVDHCVGNVGWNQMNPWVKFYEDVLGFKNILSFDDNDIST
                     EYSALMSKVMSNGNGFVKFPINEPAEGKKKSQVEEYLDFYDGEGVQHVAIATNNIIET
                     VTDLQNRGVEFLKIPPSYYETVLDRVGKIDEDLKPLSELGILIDRDEEGYLLQIFSKP
                     LEDRPTLFFEIIQRKGAKSFGKGNFKALFEALEREQDLRGNL"
BASE COUNT          643 a          346 c          266 g          792 t
ORIGIN      
        1 ccatattgtt ttattattgt aatgatgata ataaactgac gccttcttct ttaattgcag
       61 catcgtcggc tgttcttaat ggaagttttt ttaacttttg taaaacttct attgctttgg
      121 tagggttgtt atcttgtata tatgctttag caagttgtaa ataatttatc atataatatt
      181 gatcgtacaa acggcatagt tctaaattct taatggcatc ttctaaacta tactttggca
      241 atgtaccata aaacaattta acagcagttt ttttaaggcc agataagtta accatttcta
      301 aataccaatt acctaaaata aaatgtgcct ttgcatgctt gttgttcagt tgaattgctt
      361 tttctgcaaa ttgttttgta gtattaatgt aggctcctaa tgctttattt tctttttcta
      421 ttaaaatata gttactattt actagggcaa taacatagca ggcatctgca tgtgatgaat
      481 ctaccgaaaa tgctctgttt gcaaaggaat tagcagattc aaagtataat tttttatctt
      541 ttaatgcagg tagccttgcc cctattgcaa tacttagttc agatgctttt atgagagctt
      601 taaaattatc cccatatata gttaatactt ctttatattt atttaaagca tcaacctctt
      661 ttaatttaaa ttctaacttt tcagcttctt taagtaaaga atctatcttt tgagcattgg
      721 tatttttaaa aattattatt aaaccaaaag caataaaata cttccaaaat attttgttca
      781 tggtaatttt aatagaaact taatttataa attacctctt aaatcttgct ctctctctaa
      841 ggcttcaaat aatgctttaa aatttccttt accaaagctt ttagctcctt ttcgttgtat
      901 aatttcaaaa aataaagtag gcctatcttc taatggtttt gaaaaaattt gtaataagta
      961 gccttcttca tctcggtcta ttaaaatgcc taattcactt agtggtttta ggtcttcatc
     1021 aatttttcca actctatcta aaactgtttc ataatagctt ggtggaattt ttaaaaattc
     1081 taccccccta ttttgtaaat ctgtaactgt ttcaataata ttatttgttg caatagctac
     1141 gtgttgtaca ccttctccat cataaaaatc taaatactct tctacttggc tttttttctt
     1201 gccttctgct ggttcgttga tagggaattt tacaaatccg tttccattgc tcattacttt
     1261 actcattaat gctgagtatt ctgtagaaat atcgttgtca tcaaaactta aaatattttt
     1321 aaatcctaaa acatcttcat aaaacttaac ccatgggttc atttgattcc aaccaacatt
     1381 tcctacacaa tgatctacat ataatagtcc tgtacttgca gggttataag ttgaattcca
     1441 ttttacatag ccaggcataa atgcaccttt gtaatttttt ctttcaacaa aaatgtgtac
     1501 tgtatcgcca taagtatgaa taccacttaa cacaacttct ccatcattat cttttaaggt
     1561 aacaggctct aaataacttt taccgcctct tttggttgtt tgatgccatg catctgtagc
     1621 atcatctact cttaatgcca aaacctttac accatcgcca tgtttataaa tatgatctgc
     1681 aatggggtta tttgttttta aaggagtagt aagaacaaaa gttaacttgt tttgactaat
     1741 tacatagctg gctttatctt ttacacctgt ttctgggcca gcataagcca cactttgaaa
     1801 accaaaagca gttttataga agtgagctgc ttgtttggca ttacctacat aaaactctac
     1861 ataatcggta ccatgcaatg gtaaaaaatc atttgctgtt tgatctgcaa ctttccttgc
     1921 tactgatgtt tccataattg aaataatatt aaaataataa tttaaaaaag cgtgtcaaat
     1981 gtagtttatt taagaatatt atacatggat aaataattaa attaacttaa aaaagctgaa
     2041 tatgatt
//
LOCUS       DAOQPK010000324         2012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_51502_length_2012_cov_4.540112, whole genome shotgun sequence.
ACCESSION   DAOQPK010000324 DAOQPK010000000
VERSION     DAOQPK010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51502_length_2012_cov_4.540112"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="PLU36_10745"
     CDS             <1..902
                     /locus_tag="PLU36_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HMZ47273.1"
                     /translation="YHWHKDVHLADLGNIKIGTTIQDTYAALKTVVRELINNNKKVII
                     LGGSHDVTTAQYGAYSALNKIIEVTNIDATIDLDIESNLLSNYFLMEMLCGSPNFIKH
                     YNHIGFQSYLVHPTMLETIDKLRFDCFRVGKVKENIEEMEPVIRNSNMVSFDIASIQN
                     AYAPYNKVSPNGFTGEEACTLMQFAGLSNNVSTVGIYGFNNAKEDVHNLTAKQISQML
                     WYFMDGVYKSKHESAIEHSQNFNEFHLFLAGEPASFLQSKRTGRWWMQLPDGKYIACS
                     QSDYILAASEELPERWLRAIERS"
     gene            complement(909..>2012)
                     /locus_tag="PLU36_10750"
     CDS             complement(909..>2012)
                     /locus_tag="PLU36_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ47274.1"
                     /translation="VATGNYHELIKNKNSLTGKYLSKELQINFNQKERKFFDKITIEG
                     ARQNNLKNITVDFPLNVICVVTGVSGSGKTTLVKQILYPALQKIKGEFAEMPGLHKCI
                     KGSIDVVSQIEMIDQNPIGKSSRSNPVTYIKAYDAIRTLFSNQPLSKIRGFQPKHFSF
                     NVDGGRCDTCKGEGEQIIEMQFLADVHLTCEVCNGKRFKEEVLEVLINNKNIDDVLQM
                     SVEEAILYFETVKDGKQVAKALQPLQEVGLGYIKLGQSSSTLSGGEAQRVKLASFLGR
                     GKGHGNILFIFDEPTTGLHFHDIKKLLASFNALIEQGHSLIIIEHNTDVIKCADWVID
                     LGPEAGDGGGNLVFAGTPSQLKKCKESYTGKYV"
BASE COUNT          666 a          334 c          296 g          716 t
ORIGIN      
        1 tatatcattg gcacaaagat gttcatcttg ctgatttagg aaatattaaa ataggtacaa
       61 ctattcaaga tacctatgct gcattaaaaa ctgtagtaag agaattaatt aataataata
      121 aaaaagtaat tattcttgga ggtagccacg atgtaactac agcacaatat ggagcttatt
      181 cggctttaaa taaaataata gaagtaacca atattgatgc tactatagat ttagacattg
      241 aaagcaactt attaagtaat tattttttaa tggaaatgct ttgcggcagc cctaatttta
      301 tcaaacacta taaccacatt gggtttcaaa gttatttagt acatcctacc atgttagaaa
      361 caattgataa attaaggttt gattgtttta gagtaggaaa agtaaaagag aatattgaag
      421 aaatggaacc tgtaataaga aatagcaata tggtaagttt tgatattgct tctattcaaa
      481 atgcttatgc accttacaat aaagtttctc ccaatggatt tacaggagag gaagcttgta
      541 cccttatgca gtttgcaggt ttaagtaata atgttagtac agttggtatt tatggattta
      601 ataatgctaa agaagatgtt cataatttaa ctgctaaaca aataagccaa atgttatggt
      661 attttatgga tggtgtttat aagagcaaac atgaatcggc cattgagcac tcacaaaatt
      721 ttaatgaatt tcatttattt cttgcaggcg aacctgcctc ctttttacaa agcaaaagaa
      781 caggaagatg gtggatgcaa ttgccagatg gaaaatatat tgcttgcagc cagtcagatt
      841 atattttagc tgcatccgaa gaattgcctg aacgatggtt aagagccatt gaacgtagct
      901 aaattatttt atacatattt tcctgtataa ctctccttac acttttttag ttgagaaggt
      961 gtgcctgcaa atacaagatt tccaccacca tcaccagctt ctgggccaag atcaattacc
     1021 caatctgcac atttaattac atctgtatta tgctcaataa taattaagga atggccttgc
     1081 tcaattaatg cattaaaaga ggcaagtaat ttttttatat catgaaaatg aaggccagta
     1141 gtaggttcat caaaaataaa aagtatatta ccatgcccct ttcctcttcc taaaaatgaa
     1201 gctaatttta cacgctgtgc ttctccacca gataatgtgc ttgagctttg tcctaattta
     1261 atatatccta atcctacctc ttgcaaaggt tgtaatgctt tagctacttg ttttccatct
     1321 tttactgttt caaaataaag tatagcttcc tctacactca tttgtaacac atcatcaatg
     1381 tttttattat taatcaaaac ttctaacact tcctccttaa atctttttcc attacacact
     1441 tcacaagtta aatgcacatc tgctaaaaat tgcatttcaa taatttgttc tccttctcct
     1501 ttacaggtat cacatctgcc tccatctaca ttaaatgaaa aatgtttggg ttgaaagcct
     1561 cttattttag ataatggttg gttcgaaaat aatgttctaa tagcatcata agcttttata
     1621 taagtaacag ggttgcttct tgatgatttg ccaattggat tttgatcaat catttctatt
     1681 tgagaaacta catctatact gcctttaatg catttatgca ggcctggcat ttctgcaaac
     1741 tctcctttaa ttttttgtaa agcaggatat aaaatttgtt ttaccaaagt agttttacca
     1801 ctaccactta cacctgttac cacacatatt acatttaaag gaaaatctac agtaatattt
     1861 tttaagttat tctgccttgc tccttcaatt gttattttgt caaaaaattt tctttccttt
     1921 tggttaaaat taatttgaag ttctttactt aaatatttac cagttaatga gtttttattt
     1981 ttaattaact catggtaatt acctgttgct ac
//
LOCUS       DAOQPK010000325         1999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_52132_length_1999_cov_4.110597, whole genome shotgun sequence.
ACCESSION   DAOQPK010000325 DAOQPK010000000
VERSION     DAOQPK010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52132_length_1999_cov_4.110597"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PLU36_10755"
     CDS             <1..236
                     /locus_tag="PLU36_10755"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014221694.1"
                     /note="Catalyzes the reversible hydrolysis of the amide
                     bond within dihydroorotate. This metabolic intermediate is
                     required for the biosynthesis of pyrimidine nucleotides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HMZ47275.1"
                     /translation="NRGYIKEGYFADLVLVNCNQPYTINKENILYKCAWSPLEGFTFP
                     ATITHTFVNGNVVYNNGIFKEEFKGSRLLFERK"
     gene            262..1578
                     /locus_tag="PLU36_10760"
     CDS             262..1578
                     /locus_tag="PLU36_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HMZ47276.1"
                     /translation="MQTDKVTLQDLSIFNADESLSVLHYLNFTTTNRGKPFLYNILSH
                     PLSTIEEIEDTQTTIQYLNTLIPKWNIKITNGTLMVVEKFYETAFNHYPTNPSTINSF
                     FYKLTNNADFSLTKYSVTHCVYFLKGINQVLLFLSDAKSKKLLFYKEKLSLILNKTIV
                     QEIILIDDVKKIKNTSILKYAYFLKHEFKHNMQQLIVVFEQLDAYLSLAKACEKFQLT
                     FPKFIESDQPFIEAEGLFHILLPKPVSYTICLNNSENFLFLTGANMAGKSTFIKSVGI
                     AVYLAQLGLGVPAKKMTISLLDGMLSNIQISDNIINGESYFYNEVQRIKQTVEKISGN
                     KKWLVLIDELFKGTNVQDAMKCSSIVVEGLLKSSKTLFILSTHLYEIAEDLKKHSNIQ
                     FKFLETTLEKEQLVFNYQLRNGISEDRIGYVILKKEGVIEMLNKLN"
     gene            complement(1575..1868)
                     /locus_tag="PLU36_10765"
     CDS             complement(1575..1868)
                     /locus_tag="PLU36_10765"
                     /inference="COORDINATES: protein motif:HMM:NF015069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HMZ47277.1"
                     /translation="MNLLFTFLSSQIELIGYIGSFLTSITFLPQVFQAWKTKSVGDLS
                     IWMISIVIISTIVWLVYGFGIKGGGPVIVANFIVLALSLVLLYFKLSFKKNKN"
BASE COUNT          774 a          283 c          276 g          666 t
ORIGIN      
        1 aaaacagagg ctatattaaa gaaggttatt ttgccgattt ggttttagta aattgtaacc
       61 aaccatatac cataaacaaa gaaaatattt tatacaaatg tgcttggagc ccattagaag
      121 gctttacttt tcctgcaaca attacacata cttttgttaa tggtaatgta gtatataata
      181 atggtatttt taaggaagag tttaaaggaa gtagattatt gtttgaaaga aaataattag
      241 ctcatatttt ataccttgta aatgcaaaca gataaagtaa ccctgcaaga tttatctatt
      301 tttaatgccg atgaaagttt atctgttttg cattacttaa actttactac cactaacaga
      361 ggaaaacctt ttctatacaa tattttaagc catcccttat ctaccattga agaaattgaa
      421 gatacgcaaa caaccattca atatctaaat acattaatac caaaatggaa cattaaaata
      481 accaatggca ctttaatggt tgtggaaaaa ttttatgaaa ctgcatttaa ccattaccca
      541 acaaacccta gtacaataaa tagttttttt tataaactaa ccaataatgc agacttctct
      601 ttaaccaaat attccgtaac acattgtgtt tattttttaa aaggaataaa tcaagtttta
      661 ttgtttctat cagatgcaaa aagcaaaaag ctactttttt acaaggaaaa actaagctta
      721 attttaaaca aaactattgt acaagaaatt attttgatag atgatgtaaa gaagattaaa
      781 aatactagca tattaaaata tgcatacttt ttaaaacatg aattcaaaca taatatgcaa
      841 caactcattg ttgtgtttga acaattagat gcctatttaa gtttagcaaa agcctgcgaa
      901 aaatttcaat taacttttcc aaaatttatt gaatctgatc aaccatttat tgaagctgaa
      961 ggtttgtttc atattttatt accaaagcca gtaagctata ctatttgttt aaacaattca
     1021 gaaaattttt tattccttac aggtgcaaac atggcaggta aaagcacttt tataaaatcg
     1081 gttggcattg ctgtttattt ggcacaactt gggttaggag tgcctgccaa aaaaatgacc
     1141 attagtttgt tggatggtat gctaagcaat attcaaataa gcgataatat tattaatggc
     1201 gaaagttatt tttataatga agtacaaaga attaaacaaa cagtggagaa aatttctggc
     1261 aacaaaaaat ggctagtttt aattgatgaa ctttttaaag gtactaacgt acaagatgca
     1321 atgaaatgta gtagtattgt tgtagaaggt ttattaaaat ctagcaaaac attatttatc
     1381 ctatctacac atttgtacga aattgccgaa gatttaaaga agcattcaaa cattcagttt
     1441 aaatttttag aaacaaccct tgaaaaggaa caattagtat ttaattatca attaagaaat
     1501 ggaattagcg aagatagaat tggctatgta attttaaaaa aagaaggtgt aattgaaatg
     1561 ctaaataaac taaactaatt tttattcttt ttaaaactta gcttaaaata tagcaatact
     1621 aaagacaagg ctaatactat aaaatttgca acaataaccg gacctccacc tttaattcca
     1681 aatccataaa caagccacac tatagtactg atgattacaa tagaaatcat ccaaatactt
     1741 aaatctccta cgcttttggt tttccatgct tgaaaaacct gaggaagaaa agttatagat
     1801 gttaggaatg aaccaatata accaattaac tcaatttgag aggataaaaa agtaaaaagt
     1861 aaattcatag gtttataaaa caggctgttt caaaaaaaat attttttttg aaacagccta
     1921 ctaatttatt gttttactaa tttctctgtg cttctattgc ctttgctgtc tgttacttct
     1981 actaaatatg tgcctgctg
//
LOCUS       DAOQPK010000326         1999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_52142_length_1999_cov_3.455761, whole genome shotgun sequence.
ACCESSION   DAOQPK010000326 DAOQPK010000000
VERSION     DAOQPK010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52142_length_1999_cov_3.455761"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="PLU36_10770"
     CDS             <1..925
                     /locus_tag="PLU36_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ47278.1"
                     /translation="KKVYKSYGNNIILNGFDYTFNKGERIGIIGKNGVGKSTFLNILQ
                     QLEKADSGKVNIGDTIVFGNFSQQGLEITENLRVIEYVKTFAENFPLANGGSLSAAQF
                     LELFLFTPDKQYTYISKLSGGEKKRLQLLIVLFKNPNFLILDEPTNDLDLPTLSVLEQ
                     FLETYQGCVIIVSHDRYFMDKLVDHLFIFEGNGIIKDFPGTYTQYRIWQKEQDKTEEK
                     VVVKPTQTSNSTNQTNINKKKASFKEKREFEILEKEISALEKEKEALTTELSNTTNNY
                     ETIEKISTRLLEINNHLEVKEMRWLELSELL"
     gene            complement(960..1033)
                     /locus_tag="PLU36_10775"
     tRNA            complement(960..1033)
                     /locus_tag="PLU36_10775"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(997..999),aa:Arg,seq:acg)
     gene            complement(1109..1618)
                     /locus_tag="PLU36_10780"
     CDS             complement(1109..1618)
                     /locus_tag="PLU36_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47279.1"
                     /translation="MKITSIYQVLNFLLLPFALLLGFTAFPMIIAALANATALLPLFM
                     IVCIIIYIYCSIRFFNRGVKQQQICNKSLKDWIKVNAYVCIAFVLLMFVQGSVIAANP
                     MLISDQLMADLAKQSNNSLPPQVNAALVGKMLKYVTYFMLFYSVVLSVHIFMSFKLIK
                     TNNHLFQEQ"
     gene            1741..>1999
                     /locus_tag="PLU36_10785"
     CDS             1741..>1999
                     /locus_tag="PLU36_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47280.1"
                     /translation="MKILIAEDELLMAKTLEKKLLLDGHEVLIANDGRTALTLYETEN
                     PAAWKLLINLIKVLNKTPEQIETVLDVDQTLWMLALNNTLVN"
BASE COUNT          796 a          283 c          304 g          616 t
ORIGIN      
        1 gaagaaagta tataaaagct acggtaacaa tattatttta aatggttttg attatacttt
       61 taataaagga gaacgaattg gaataatagg taagaatggc gtagggaaaa gtaccttttt
      121 aaatattttg cagcaattag aaaaagcaga tagtggtaaa gtaaatattg gcgataccat
      181 tgtttttggt aatttttctc aacaaggttt agaaataact gaaaacttaa gagtaataga
      241 atatgtaaaa acatttgccg aaaattttcc tttagctaat ggtggctcat taagtgcagc
      301 acaattttta gaactatttt tatttacacc agataaacaa tacacctaca ttagcaaatt
      361 aagcggaggc gaaaaaaaac gattacaatt attaatagta ttatttaaaa atcctaactt
      421 tttaatttta gatgagccaa ccaacgattt agatttacct actttatctg tattagaaca
      481 gtttttagaa acttatcaag gttgtgtaat tattgtaagc catgataggt attttatgga
      541 taaattggta gatcatttat ttatttttga aggcaatgga atcatcaaag attttcctgg
      601 aacctataca caatatagaa tttggcaaaa ggaacaagat aaaacagagg agaaagttgt
      661 tgtaaaacct actcaaacca gcaactcaac taaccaaacc aatattaaca aaaagaaagc
      721 aagctttaaa gaaaaaagag aatttgaaat attagaaaaa gagatttcgg ctttagaaaa
      781 agaaaaagaa gcccttacta cagaacttag taacactacc aataattatg aaaccattga
      841 aaaaataagc actcgattat tagaaataaa taaccattta gaagtaaaag aaatgcgttg
      901 gttagaactg agtgaattat tataagaata aaaaagctgc taattaaagc agcttttagt
      961 gaccccgtca ggattcaaac ctgaaacctt ttgatccgta gtcaaatgct ctattcagtt
     1021 gagctacgga gccatttata tcccatttaa gggctgcaaa tataaacact tgttgctaaa
     1081 aaaaacaaat taaattaatt tagaaactct attgctcttg aaataaatga ttgtttgttt
     1141 ttattagttt aaaactcata aaaatatgta cactaagtac tacagaataa aacaacataa
     1201 aataggtaac atattttagc attttaccaa ctaatgcagc atttacttga ggaggcaggc
     1261 tattgttact ttgtttggct aaatctgcca ttaactgatc ggaaattaac attgggtttg
     1321 cagcaattac acttccttgc acaaacatca acaacacaaa tgcaatacat acataagcgt
     1381 ttactttaat ccaatctttt aaagatttat tacaaatttg ttgctgcttt actcccctat
     1441 taaaaaacct tatgctacaa taaatgtaaa taattataca aactatcata aacagtggta
     1501 gtaaagcagt agcatttgct aaagcagcaa ttatcatagg aaaagcagta aaacctaata
     1561 gcagagcaaa gggtaataat aaaaaattta atacttgata tatagatgta attttcattt
     1621 agttatttta ttaaaataaa aaattgatac aataatttta tattaatgat atttgattaa
     1681 tagattgtat tttacaaata aagaaaatta tttttacttt tagctcaacc cgtatataat
     1741 atgaaaattt taattgccga agatgaactt ttaatggcta aaactttaga gaaaaaactt
     1801 ttattagatg ggcatgaagt tttaatagca aatgatggaa gaactgcatt aactctttat
     1861 gaaacagaaa atccggcggc ctggaaattg ctcatcaacc tcatcaaggt gctgaacaaa
     1921 accccggagc aaattgaaac cgtattggat gtcgaccaaa ccctttggat gctggccttg
     1981 aacaacacgc tggtcaacc
//
LOCUS       DAOQPK010000327         1996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_52280_length_1996_cov_5.098403, whole genome shotgun sequence.
ACCESSION   DAOQPK010000327 DAOQPK010000000
VERSION     DAOQPK010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52280_length_1996_cov_5.098403"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /locus_tag="PLU36_10790"
     CDS             <1..1434
                     /locus_tag="PLU36_10790"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ47281.1"
                     /translation="KSGKDINRLCFFSCDSNIVINYEAQKYLVQHEEVKQTTIAPVQH
                     FTKGTDDLSIFQKCVELTNNKMAYVEGNRNNYVNLLANNCNRNGLSELSATQYINSHY
                     NYNDAEVSQTIKSAYHSHTAEHGKFSKQSTPVVPIKDFSNTGAVPLKQLIHRANTEPD
                     TPFIWSGIKENSFGFIFGPSKSGKTTICENLAMALVAGLTEFMGQSITKGDYKVFFLS
                     LEEYWKPRSQRNAKQTQYLIEHIGNDNWLDNYSANDDTVPRQISSEEEWLLLETLIKK
                     NGANFVIIDSLSRLYEGGIEDSGLAKKVALRLRELCNRLKITLIIIHHTPKQIGRPIT
                     IDSLAGSRMLAQEADFMIGVGKSPEGKRYIKDVAFRYKQEDSENVMTFDINSYQYAVE
                     GIQLPETVLLKETDGREDSTNADAILEYIDEKSKSQIGGCYLKDLTEHFVTGKIISRA
                     TLFNCLEKLQKDGKISKPSKGQYKSTQ"
     gene            1556..1846
                     /locus_tag="PLU36_10795"
     CDS             1556..1846
                     /locus_tag="PLU36_10795"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ47282.1"
                     /translation="MSNHFGFRIRQLREHNNLLLRQVAPFIDIDTPLLSKIERGERVL
                     KKEQIPLLAKILKTDEDELMTLWLADQMYNVIDGEPLANEAIKQLTKNIKKP"
BASE COUNT          741 a          363 c          351 g          541 t
ORIGIN      
        1 aagtcaggca aggatataaa ccgactatgt tttttcagtt gcgatagtaa tattgtaatc
       61 aattacgaag cccaaaaata cttagtacag cacgaagaag taaagcaaac aactattgca
      121 ccagtgcaac attttacaaa aggtacagac gacctaagta tttttcaaaa atgcgtagag
      181 cttaccaata acaaaatggc ttatgtagag ggcaatagaa acaattatgt aaatctatta
      241 gccaataatt gcaatcgtaa tggcttatcc gaattgtcag caactcagta cataaattca
      301 cattacaatt acaacgatgc agaagtaagc caaacaatca aaagtgccta ccatagccat
      361 acagcagaac acggtaaatt ttccaagcaa tctacgccag tagtaccaat aaaagatttt
      421 tctaacacag gagcagtacc actaaaacaa ctcattcaca gagccaatac cgaacctgat
      481 acacctttta tttggagtgg cattaaggaa aattcctttg gttttatttt tggaccttcc
      541 aaaagtggta aaactaccat ttgcgaaaac ttggctatgg cattggtggc agggcttaca
      601 gagtttatgg gtcaatccat taccaaaggt gattacaaag tgttttttct atccttagag
      661 gagtattgga aaccaagaag ccaacgaaac gccaaacaaa cacaatacct tatagagcat
      721 attggtaacg ataattggtt agataactat tcagcaaatg atgatacagt acctcgtcaa
      781 atttcaagtg aagaagaatg gttattatta gaaacattaa tcaaaaaaaa tggtgcaaat
      841 ttcgtcatta tagacagttt aagccgtctt tacgaaggtg gtatagaaga tagtggatta
      901 gctaaaaaag tggctctacg gctacgagaa ttgtgcaata gattaaaaat taccctcatt
      961 atcatacacc atacacccaa gcaaataggc agacccatta ccatagatag tttggcaggt
     1021 agtcgtatgt tagcacagga agccgatttt atgataggcg taggcaaaag cccagagggt
     1081 aagcgttata taaaagatgt tgcatttcgt tacaagcaag aagatagcga aaatgttatg
     1141 acctttgata taaacagcta tcaatatgca gtagagggca ttcaattacc tgaaaccgtt
     1201 ttactcaaag aaacagacgg cagagaggac agcaccaatg cagatgctat tttagaatac
     1261 attgacgaga aatctaaatc acaaataggt ggttgctatt tgaaagacct tacagagcat
     1321 ttcgttacag gcaaaataat ttcaagagct actttgttca actgccttga aaaacttcaa
     1381 aaagacggca aaatatccaa gccctcaaaa ggacagtata agtccaccca ataataaccc
     1441 cctacttttt acttagacta cttagactgc tcaaagtttt gcacttcatt actttggttt
     1501 gctttattat gcttatattt gtcaaatatt ggcaagtcaa aataagcaat tcaatatgtc
     1561 taatcatttt ggttttagaa taaggcaact tcgggaacat aacaacctgc tacttcggca
     1621 ggttgcccca tttatagata ttgatacacc tcttttgagt aaaatagaac gaggcgaaag
     1681 agtattaaag aaagaacaga taccactatt agccaaaata ttaaagactg atgaagatga
     1741 gttaatgacc ctatggcttg ccgaccaaat gtataatgta atagatggag aaccattagc
     1801 aaacgaagca attaaacaac ttactaaaaa tattaaaaag ccctaattta ttgtatatgg
     1861 cagaacaaaa cttaatatta aatatcctaa atttttcaca cccagtacaa acagtagagt
     1921 gttcttttca attggcaaaa gccgatggct tttaccctgt taaagtcagt agcttaccag
     1981 tcaatattga agacct
//
LOCUS       DAOQPK010000328         1966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_53831_length_1966_cov_3.538985, whole genome shotgun sequence.
ACCESSION   DAOQPK010000328 DAOQPK010000000
VERSION     DAOQPK010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53831_length_1966_cov_3.538985"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="PLU36_10800"
     CDS             complement(<1..1032)
                     /locus_tag="PLU36_10800"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HMZ47283.1"
                     /translation="MYKLLLLIIFIPSITLNAQSKKKKHKNKLAAEQKINAVLIKNLQ
                     QHVQYLASDSLEGRRTGTKGEIAAMNYIVKQYTQEGIEPKGTTGYIQEFEINEGKKVD
                     ENSYLKINNVNLALHKDYFPLATSNNGKFTTSTAAIALKEISEPWFIDVKEELEANKN
                     NPHFDINVYIKEEATNATKKGALALFFYNSSNITDNIMFNKNDPSAIEQIPIIYIKKE
                     AIEKYFKDVSATFKLEYKIAIAPSIRKARNVVAYINNHAANTIILGAHYDHLGYGEDK
                     NALDALHEVHNGADDNASGTAALIELAKMLKHSNAKNNNYLFIHFSGEELGLFGSKYW
                     IENPSINITP"
     gene            complement(1105..>1966)
                     /locus_tag="PLU36_10805"
     CDS             complement(1105..>1966)
                     /locus_tag="PLU36_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018617143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMZ47284.1"
                     /translation="NYLKEKINQFKKLNQPIEWINPLDSTQKNKVFYGESKLLKEVRY
                     FPMIQLLVAGLFVLIIVATQYVSYKSTQNQVWAGLAKETAHQLGTPISSLQGWVEMLK
                     EINGNEKIVNEIDKDINRLLLISDRFSKIGSKPNMEEKNIIEQIENMIDYIKKRAGAK
                     VFFKFDSNNETDLPVMISPPLFDWVIENLLKNALDAMDGKGNISVKIIDTASNIIIDV
                     SDNGKGISKKNIGKVFKPGFTTKKRGWGLGLTLTKRIIEHYHKGQIFVQHSEPNIGTT
                     FRIMLPKSNV"
BASE COUNT          590 a          307 c          260 g          809 t
ORIGIN      
        1 tggagtaata tttatagaag ggttttcaat ccagtattta ctaccaaaca atcctaactc
       61 ctctccacta aaatgaatga ataaataatt attatttttt gcattggaat gcttaagcat
      121 tttagctaac tctattaaag ctgcagtgcc actagcatta tcatctgcac cattgtgcac
      181 ttcatgtaaa gcatctaaag catttttatc ttcgccatat cctaagtggt cataatgtgc
      241 tccaagaata attgtattgg ctgcatggtt gttaatataa gctactacat ttctggcctt
      301 tcttatgctt ggtgcaatag caattttata ttctaatttg aatgttgcac ttacatcttt
      361 aaaatatttt tcaatagctt ctttcttaat atatataatt ggaatttgtt caatagcaga
      421 tggatcgttt ttattgaaca ttatattatc tgtgatatta gaggaattgt aaaagaacaa
      481 tgctaatgct ccttttttag tggcattggt tgcttcttct tttatataaa cgttaatatc
      541 aaaatgtgga ttgtttttat tggcttctaa ttcttcttta acatctataa accaaggttc
      601 gctaatttct tttaaggcaa ttgctgcagt agatgttgta aattttccat tattacttgt
      661 tgcaagtgga aaataatctt tatgtagagc caagttgaca ttgttaattt ttaaataaga
      721 attttcatct actttttttc cttcattaat ttcaaattct tgaatatagc cagtagtgcc
      781 tttgggttca ataccttctt gtgtatattg tttaacaata taattcattg cagcaatttc
      841 accctttgta cctgttcttc ttccttctaa agaatcgcta gctaaatatt gcacatgttg
      901 ttgaagattt ttaattagta cagcatttat tttttgttca gcagcaagtt tatttttatg
      961 cttcttcttt ttgctttgtg catttagtgt aatagatggt ataaatataa ttagaagtaa
     1021 aagtttgtac atgtttaaaa taatttttac aaaaatagga gttgtagtta catataaaag
     1081 taatagaaat gtaaaattgc tttgttaaac gttactttta ggaagcatta ttctaaaggt
     1141 agtaccaata ttaggctcac tatgttgtac aaatatttgt cctttatgat aatgttcaat
     1201 aattcttttg gttaaagtta ggcctaagcc ccatcctctt tttttggtag taaatcctgg
     1261 tttaaaaact tttccaatat tttttttact aattcctttt ccattatccg aaacatcaat
     1321 aataatatta cttgctgtat caataatttt tacagatata ttgcctttac catccatagc
     1381 atctagtgca ttttttaata aattttcaat tacccaatca aataatggtg gcgaaatcat
     1441 tacaggtaaa tctgtttcat tattagaatc aaacttaaaa aacactttag caccagccct
     1501 tttcttaata taatctatca tgttttcaat ttgctcaata atattttttt cttccatatt
     1561 gggcttacta ccaattttac taaacctgtc tgatattagt aataatctat taatgtcttt
     1621 atctatttca tttactattt tttcattgcc atttatttct ttcaacattt ctacccaacc
     1681 ttgtaaactt gaaattggag tacctaactg atgtgcagtt tctttagcca agcctgccca
     1741 aacctggttt tgtgtgcttt tatacgaaac atattgtgtg gctactatta ttaaaacaaa
     1801 caaacctgca accaataatt gtatcattgg aaagtagcga acctctttca agagtttgct
     1861 ttctccatag aatactttat ttttttgagt gctatctaat gggttaatcc attcaattgg
     1921 ttggtttaat tttttgaatt ggttaatttt ttcttttaaa taattc
//
LOCUS       DAOQPK010000329         1953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_54547_length_1953_cov_4.632771, whole genome shotgun sequence.
ACCESSION   DAOQPK010000329 DAOQPK010000000
VERSION     DAOQPK010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54547_length_1953_cov_4.632771"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1642)
                     /locus_tag="PLU36_10810"
     CDS             complement(<1..1642)
                     /locus_tag="PLU36_10810"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HMZ47285.1"
                     /translation="MAEVILMPRLSDTMTEGVIAAWHKQVGDLVNVGEVLAEIETDKA
                     TMELESYKQGTLLHIGTPNGGKLQVNDLLAIIGNKEEDIAALIAQHSLGNKTANTTTV
                     ILPTTNESISKETIQTPTPSSKVNFDEVVLMPRLSDTMTEGVIASWQKNIGDTVKKGE
                     VLAEIETDKATMELESYKDGILLYHGANAGEKIQVNDLLCIIGSNATDVAAILASVKN
                     GSHVATAATTSQPAIPLDSNANTTSTSSNNTQTNVNEGRVFASPLAKKIAIDKGIDLK
                     NVKGSGDNGRIIKSDIETYQPPQATTSLNITETKTTTPQFTATGVESFNEVPVSQMRK
                     TIARRLGESKFTAPHFYLTMRIDMDAAVEARTKLNADGKTKISFNDMVLKAVAIALKQ
                     HPKVNSSWLEDKIRYNHHINIGVAVAVEEGLLVPVVRFADTLSLTQISSIVKDYAQKA
                     KDKKLQPSDWEGNTFTISNLGMYGIDQFTAIINPPDACILAVGGIAQVPVVKNGMVVP
                     GNVMKVTLSCDHRVVDGATGSAFLQTLKSEELEESLYLI"
BASE COUNT          587 a          363 c          291 g          712 t
ORIGIN      
        1 gaattaaata caggctctct tcaagttctt cactttttaa tgtttgtaaa aatgcagagc
       61 cagtagcacc atctacaacc ctatggtcgc aacttaaagt aactttcatt acattgccag
      121 gtactaccat tccatttttt acaactggca cttgtgcaat accgccaact gctaaaatac
      181 aggcatctgg tggattaata attgcagtaa attggtcaat accatacatt cctaaattac
      241 taattgtaaa agtatttcct tcccaatcag atggttgaag ttttttgtct ttagcctttt
      301 gggcatagtc ttttacaatg gaagaaattt gggttaaact taacgtatct gcaaagcgta
      361 caacaggtac taataaacct tcctctacag ctacagcaac gccaatatta atatgatggt
      421 tataacgtat tttatcttcg agccaactgc tattaacttt tggatgttgt tttaaagcaa
      481 tagcaactgc ttttaaaacc atatcattaa agctaatttt tgttttgcca tctgcattta
      541 atttagttct ggcttccaca gcagcatcca tatcaatacg cattgttaag taaaaatgag
      601 gggcagtaaa tttgctttcc cctaatcttc ttgcaatggt tttacgcatt tggctaactg
      661 gtacttcatt aaaactttct acgccagtag cagtaaattg tggagttgta gttttcgttt
      721 ctgtaatatt taaactggtt gtagcctgtg gtggttgata ggtttcaata tctgatttta
      781 ttattctacc attatcgcca ctacctttta cattttttaa atctatgcct ttatctatag
      841 caattttttt agccaatggc gaagcaaaaa ctcttccttc atttacatta gtttgtgtat
      901 tattggatga agtagaagtt gtatttgcat tactatctaa tggtatagca ggttgagaag
      961 ttgtggcagc agttgctacg tggctaccat tttttacaga tgcaagaatt gcagcaacat
     1021 ctgttgcatt gctacctata atacaaagta aatcatttac ctgaattttt tctcctgcat
     1081 ttgcaccatg atacagcaaa attccatctt tatagctttc taattccata gtggctttat
     1141 ctgtctcaat ctctgctaac acttctcctt tcttaactgt atcaccaata tttttttgcc
     1201 aacttgcaat tacgccttct gtcatagtat cgcttaaacg aggcattaaa actacttcat
     1261 caaagtttac ttttgatgat ggtgttggtg tttgtatcgt ttcttttgaa atgctttcat
     1321 tagtagtagg caaaataaca gttgtagtat ttgcagtttt gttacccaaa ctgtgttgtg
     1381 ctattaatgc agcaatatct tcttctttat ttccaataat ggcaagcaaa tcatttactt
     1441 gaagtttgcc accatttggt gtaccaatat gtaacaaagt gccttgtttg tagctctcta
     1501 attccattgt tgctttatca gtttcaatct ctgcaagtac ttctcctaca tttaccaaat
     1561 cgccaacttg tttgtgccaa gctgctatta caccttcagt catggtatcg ctaaggcgag
     1621 gcattaaaat aacttcagcc ataaaatatt ttaattatac ttttttaatc ttttagagcc
     1681 gtgaaaatac agtaaaataa agttttgaca agtattcctc aaatttttgt tattaaaaat
     1741 caacaaatag aaataaatag ctcaattttt aataaaattt gaaagaaata aaacctgttt
     1801 tttaaagttt tgactattag gatgaaaaac ttacattact tttttccatt ttaatcttaa
     1861 actatttact gctaaaacaa catcacttaa gcccattatt aaagccccaa aagttggact
     1921 taaaaagcct aacgcagcaa caggtatggc aac
//
LOCUS       DAOQPK010000330         1937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_55444_length_1937_cov_4.437832, whole genome shotgun sequence.
ACCESSION   DAOQPK010000330 DAOQPK010000000
VERSION     DAOQPK010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55444_length_1937_cov_4.437832"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="PLU36_10815"
     CDS             complement(<1..1065)
                     /locus_tag="PLU36_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012793470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HMZ47286.1"
                     /translation="MQFIPQILFLIITGIAIYLFYKKASEIRRNILLGKEEDFSDNKN
                     KRWNNMILLALGQKKMFRNPIVALLHFIVYAGFIIINIEVLEIVLDGILGKHRLFAGL
                     LPMGLYQFLINSFEILALLVLTACVVFLIRRNIIKVKRFTGNDLKGWAYNDANYILFT
                     EIVLMSMFLIMNASDTLLQERNFATYAQHSTGNFIISSYIQPLINSFSDNSLVLIERV
                     CWWLHIIGIFAFLNYLPYSKHLHVLLAFPNAYYARLEPKGKMKNMPSVQNEVLYAMQP
                     ELAPTNAAPPERFGAKDVFDLSWRNLLDAYSCTECGRCTAACPANSTGKLLSPRAIMM
                     KTRDRLEEVGRNITQNKTFIE"
     gene            1205..1705
                     /locus_tag="PLU36_10820"
     CDS             1205..1705
                     /locus_tag="PLU36_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018626088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HMZ47287.1"
                     /translation="MPDKKYILNKQEASLKIKRLALEVAEILISNKNEIIIIGIKKNG
                     VTIAEKVAELLKQYVQQPLTVLQLSLNKATANFIELSENIDFTNKYVLLCDDVVNSGK
                     TLLYALKPILEQYPASIQTLVLVERMHTLFPIKADFVGISLTSTLQNNIVVEIENNEI
                     TGAYLE"
     gene            complement(1716..>1937)
                     /locus_tag="PLU36_10825"
     CDS             complement(1716..>1937)
                     /locus_tag="PLU36_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HMZ47288.1"
                     /translation="DMNLNFDGTSINVTKIMSTDAPRRIAGIVTELNFTEKFTPSNEQ
                     KEQLIRIARSCPVEKSLHPDVQLTVTFNW"
BASE COUNT          697 a          283 c          272 g          685 t
ORIGIN      
        1 ttctataaaa gttttatttt gagtaatgtt tctgcctact tcttctaatc ggtctcttgt
       61 tttcatcata atagctcttg ggcttaaaag tttacctgtt gaatttgcag ggcaagcagc
      121 agtacatcgt ccacattcgg tacaactgta tgcatctaat aaatttctcc agcttaaatc
      181 aaaaacatct ttggcaccaa atctttctgg aggtgctgca tttgtaggtg caagctctgg
      241 ctgcattgca tacaaaactt cattttgtac gctgggcata tttttcattt ttcctttagg
      301 ctctaacctt gcataatagg cattaggaaa agctaacaaa acatgcaaat gtttactata
      361 tggtaaatag tttaagaaag caaatatgcc aataatatgt aaccaccaac atactctttc
      421 tattaaaact aaactgttat ctgaaaaact atttataaga ggttgtatat aagaagaaat
      481 aataaaattg cctgtggaat gttgagcata ggttgcaaaa tttctttctt gaagaagagt
      541 atcactggca ttcattatta aaaacatact cattaaaact atctcagtaa ataagatata
      601 attggcatcg ttataagccc aaccttttaa gtcgttgcca gtaaaacgtt ttactttaat
      661 aatatttcta cgtataagaa atacaacaca agctgttaat accaataagg ctaaaatttc
      721 aaaactatta attaaaaact ggtataaacc cataggcaat aagccggcaa acaacctatg
      781 ttttcctaaa atgccatcta aaactatttc taaaacttca atattaataa ttataaaacc
      841 agcatataca ataaaatgca aaagagcaac gattgggttt ctaaacattt ttttttgacc
      901 aagagccaat agaatcatat tgttccatcg tttattttta ttgtcgctaa aatcttcttc
      961 tttacctaat aaaatgtttc ggcgtatttc acttgctttt ttataaaata aataaattgc
     1021 aatacctgtt ataattaaaa ataaaatttg tggtataaat tgcatataaa ttaaattaaa
     1081 atattaaaat agcttttcaa agataagttg taaattatta tttaatacaa tacaattcag
     1141 tgtattaaaa cattacttgt acattcattt atttgtattt attttgaggt agaaaaatta
     1201 agtaatgcca gataaaaaat atattttaaa taagcaggaa gcctctttaa aaattaaaag
     1261 attggcttta gaagttgccg aaatattaat aagtaataaa aatgaaatta ttattattgg
     1321 tattaagaaa aatggagtaa ccatagccga aaaagtagct gagttgttaa aacagtatgt
     1381 acagcaacct cttactgtgt tgcagttaag tttaaataaa gccactgcaa attttataga
     1441 gctttcagaa aatattgatt ttactaataa atatgtttta ttatgcgatg atgttgtaaa
     1501 tagtggcaaa acattgttgt atgctttaaa gccaatttta gagcaatatc ctgcaagtat
     1561 tcaaacattg gttttggtag aacgtatgca tactctattt ccaattaaag cagattttgt
     1621 aggtattagc cttacctcta cactacaaaa taatattgtt gtagaaatag aaaacaatga
     1681 aattactgga gcctatcttg aataaaaagt atttattacc aattaaaagt tactgttaat
     1741 tgtacatctg gatgtaagct tttttcaaca gggcaacttc ttgcaattct tattaattgt
     1801 tctttttgtt cgttgcttgg tgtaaatttt tcggtaaaat ttaattcagt tacaattcct
     1861 gcaattcttc taggtgcatc ggtactcata attttggtta cattaatact ggtgccatca
     1921 aaatttaagt tcatatc
//
LOCUS       DAOQPK010000331         1876 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_58953_length_1876_cov_3.812740, whole genome shotgun sequence.
ACCESSION   DAOQPK010000331 DAOQPK010000000
VERSION     DAOQPK010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58953_length_1876_cov_3.812740"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            369..>1876
                     /locus_tag="PLU36_10830"
     CDS             369..>1876
                     /locus_tag="PLU36_10830"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HMZ47289.1"
                     /translation="MRNCYTPSFFKSLFLFAFATIATLSTKAQTYCTTGLYTTGCVDD
                     QIVSFSTTGGITNITNNNSGCGGGAAGYTFYNTMTHSTTQGGVVNFSITSDPSWTEGY
                     KIWVDYNNDGDFDDAGENVYDPATTTPAGSVITGNFTVPVSTIPGIKRMRVRCVFSSA
                     AFTPCSSVTYGEVEDYNLQVISLTPCSGIPNAGTVSATPLTGCVGVPINLTSAGIDLT
                     ASGLKYQWQVSTDGGTTWTSPVNDTFPSLTTTQVTTSQYRIIVTCTATGGGADTSNIV
                     TVTSPPIPGGNYTINKNQPTTWPPGTNFNSFADAYASLNCGISAPVVFNVVAGSGPYN
                     EQLIVNGLVPGSSATNTITFKGNGETISFNTNNANERAVIKLKKTKFFVFDSLVVNAG
                     GGTYGFGFHITQDADSNTIRKCTVNTNATATSANYAGIVISGADNNAIGTGTTTALCD
                     GNIIDSNTVNGGMYGITLTATFSGGANGFNRITNNTIKDFYTDGIHVIASYNS"
BASE COUNT          626 a          310 c          331 g          609 t
ORIGIN      
        1 caatatgcaa caatggctgc gaaactgcag tatttaagtt tacaaaatat taatttaaag
       61 gctcccaact aagggagcct ttaaaattta aggcttttat attttattaa acaataatat
      121 ttataaatac tgaataacta atttaatatt aaagcagcct tttatgacat aaatgtctct
      181 taattatgat tttttaaaaa aaattactgt ttatgtattg aatataacat taatttagcg
      241 gctcctaaat aaaaccaaaa actaaaggaa gtagaattaa taagcttatc tcaacaagaa
      301 tataattgag aagcattgtt aaaactataa ttagcttaaa accatactta tttttaattt
      361 gaattaatat gagaaattgc tacaccccct cgttctttaa aagtttattt ttgtttgcat
      421 ttgctaccat tgcaacttta agtacaaaag cccaaactta ttgtactaca ggtttatata
      481 ctactggatg cgtagatgac cagatagttt ctttttcaac aactggtggt attactaaca
      541 ttaccaataa caactctggt tgtggtggtg gggctgcagg ttatactttt tataacacca
      601 tgacccactc aacaacgcaa ggtggagttg taaatttttc aataacaagc gacccttctt
      661 ggactgaagg atacaaaata tgggtggatt ataacaatga tggagatttt gatgatgctg
      721 gagaaaatgt gtatgacccg gcaactacaa ctccagctgg tagtgttatt acaggtaatt
      781 ttacagtgcc agttagcaca attcctggta ttaaaagaat gagagtaaga tgtgtatttt
      841 catctgcagc ttttacacca tgttcaagtg ttacatatgg agaggtagaa gattataatt
      901 tacaagtaat atctttaaca ccatgttcag gtattcctaa tgctggcaca gtaagtgcta
      961 cgcctttaac tggttgtgtg ggtgtaccca ttaatttaac ctctgcaggt attgacctta
     1021 cagcctctgg tttaaaatat caatggcaag ttagcacaga tggtggaact acatggacaa
     1081 gtccagtaaa tgatacattc ccttccttaa ctacaacaca ggttacaaca agccaatatc
     1141 gtataattgt tacttgtaca gctactggtg gcggtgctga tacatcaaat attgtaacag
     1201 taacaagccc accaattcct ggtggtaatt atacaattaa taaaaatcaa ccaactacat
     1261 ggccaccggg tactaatttt aatagttttg cggatgctta tgcttcttta aactgtggaa
     1321 tatctgcacc tgttgtgttt aatgtagttg caggttctgg tccttacaac gagcaattaa
     1381 ttgtaaatgg attagtacct gggtctagtg ctaccaatac tataacattt aaaggcaatg
     1441 gcgaaaccat cagtttcaat accaacaatg caaatgaaag agcagttata aaacttaaga
     1501 aaacgaaatt ttttgtattt gacagtttag tggtaaatgc tggtggtggc acttatggtt
     1561 ttggctttca cattacacag gatgcggatt caaatactat tcgtaaatgt actgttaata
     1621 caaacgcaac tgcaacctct gcaaactatg ctggtattgt aattagtggt gccgataaca
     1681 atgcaatagg tacaggaaca acaactgctt tatgcgatgg taatattatt gattccaata
     1741 ctgtaaatgg tggtatgtat ggtattacac ttactgctac tttctctggc ggtgcaaatg
     1801 ggtttaatag aattaccaac aatactatta aagatttcta cacagatggt attcatgtta
     1861 ttgccagtta taattc
//
LOCUS       DAOQPK010000332         1862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_59773_length_1862_cov_4.330382, whole genome shotgun sequence.
ACCESSION   DAOQPK010000332 DAOQPK010000000
VERSION     DAOQPK010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59773_length_1862_cov_4.330382"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PLU36_10835"
     CDS             complement(<1..250)
                     /locus_tag="PLU36_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HMZ47290.1"
                     /translation="MFRSRTCGELTIKNVGEIVTLAGWVQTIRKFGAITFIDLRDRYG
                     ITQILFGENLNNQLEENSLGREFVLQVNGTVTERSNKNK"
     gene            complement(453..1136)
                     /locus_tag="PLU36_10840"
     CDS             complement(453..1136)
                     /locus_tag="PLU36_10840"
                     /inference="COORDINATES: protein motif:HMM:NF025221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HMZ47291.1"
                     /translation="MRRVYLALLVSCSMFFSSCDTLKSVADSILTERDAISAIKEMLT
                     IGSNGNALISKDVLMNAILPKDVNTVLQKLQQMGLSKEIDKFTNTLTNAATQTATNSI
                     PVFADGIKRMNITDAINIVKNGGTAATDYFRTTIGNDLRTTVTPIMKTALDQYQVTKE
                     WDKLVAPAKIFLGNKINLNLNLDNLLAGVVTNAMFNKIAEQEVNIRTKAEARTSPLLQ
                     RVFGKNWNN"
     gene            1247..>1862
                     /locus_tag="PLU36_10845"
     CDS             1247..>1862
                     /locus_tag="PLU36_10845"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47292.1"
                     /translation="MIRLLLFISIFFYKVNAQEMENRTVSNLSLTNTILTAPKFNTPA
                     ISKASNNFHIHYLKFKLKVNPAIRYIDGSVDIYFIPNILTDSIELDLSYQLKVDSIVF
                     NNQKLSYIQHPNNTLIIYFQTALVAAQNYSMNIFYKGVPGDSGYGSFIQTTHNNTPVI
                     WTLSEPFGSKDWFPCKNELSNKIDSVDMFITHPSVYKATANGVLQ"
BASE COUNT          621 a          302 c          240 g          699 t
ORIGIN      
        1 ttttattttt attacttctt tcagtaaccg ttccatttac ttgcaaaaca aattcacgtc
       61 ctaaagaatt ttcttctaat tggttgttca ggttttcgcc aaaaagtatt tgtgttatac
      121 cataacggtc tcttaaatca ataaaagtaa ttgcaccaaa tttcctaatt gtttgtaccc
      181 atcctgctag ggtaactatt tcaccaacgt ttttaattgt taattcacca caagttctag
      241 aacgaaacat actatttaga ttattttaat tttttgtaat caacggttgt tatataatga
      301 tacaattgtt gcgtcgcacc cttcaacttt cgatgcttta ttaggcaaag tttgtaaggc
      361 ttacagtatt tgtacaacag ttgcaaatat atattaaata aaaagagagt agcgtttaac
      421 taccctctac ttatattttt tttaaatatt atctaattat tccaattttt tccaaaaact
      481 ctttgtagta gtgggcttgt tctagcttct gctttagttc taatatttac ctcttgctca
      541 gcaattttat taaacatagc attggtaaca acaccagcta acaaattatc taaatttagg
      601 tttaagttta ttttattacc caaaaatatt tttgctggtg caaccaattt atcccattct
      661 ttagtaacct gatattggtc taaggctgtt ttcataattg gcgtaacagt tgttcttaaa
      721 tcattgccaa ttgttgttct aaagtaatct gttgcagcag ttccaccatt tttaacaata
      781 tttattgcat cggttatatt cattcttttt ataccatctg caaaaacagg tatagagtta
      841 gttgctgttt gtgttgcagc attggtaagg gtattggtaa atttatcaat ttctttactt
      901 aggcccattt gctgcaattt ttgtaaaacc gtatttacat ctttcggtaa aatagcattc
      961 attaatacat ctttgctaat taaagcattt ccattactgc caatagtaag catttctttt
     1021 atggcactta tagcatctct ttctgttaaa atactatctg ctacactttt taatgtgtcg
     1081 caactactaa aaaacatact acaactaacc aataaggcta aataaactct tctcataaca
     1141 taataaaatt aaggggtcaa acataagatt tataactaac atattgtaat aaagtattgg
     1201 tgataattta ataaaatact tttattcaat taattaattt tatgcaatga ttaggctttt
     1261 actatttatt agtatcttct tttataaagt taatgcacaa gagatggaga acagaacagt
     1321 ttccaatctt tctttaacca atactatact aacagctcca aaattcaata caccagctat
     1381 ttcaaaagct tctaacaatt ttcacattca ttacttaaag tttaaattaa aagtaaaccc
     1441 agcaataaga tatattgatg gcagtgtaga tatatatttt attccaaaca tactaaccga
     1501 tagtatagag ttagatttat cctatcagtt aaaggtagat agtatagtat ttaataatca
     1561 aaaattaagt tacatacaac acccaaataa tactttaatt atttacttcc aaacagcctt
     1621 agttgctgca caaaactatt ccatgaatat attttataaa ggggtgcctg gcgatagtgg
     1681 ctatggttct tttatacaaa ctacacataa taatacacct gttatttgga cattaagcga
     1741 accttttgga agtaaagatt ggtttccttg taaaaatgaa ttaagcaata aaatagatag
     1801 tgtagatatg tttattacgc atccttctgt ttataaagca acagcaaatg gtgtgttgca
     1861 at
//
LOCUS       DAOQPK010000333         1838 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_61178_length_1838_cov_5.339877, whole genome shotgun sequence.
ACCESSION   DAOQPK010000333 DAOQPK010000000
VERSION     DAOQPK010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61178_length_1838_cov_5.339877"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PLU36_10850"
     CDS             <1..667
                     /locus_tag="PLU36_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ47293.1"
                     /translation="NLKLVGWQWNAKNSNGKKVLIAHGFDSCSYKFDKLIQHLLSAGF
                     TVLAFDAPAHGLSDGKTVNALDYKKALLAINKQVEDLYCIIGHSLGGLAASLAAEEMH
                     ELKKLILIAPATETQRAIDNFFKYVPLGKDIKDEIEKIIYNFEQKPVSYYAVSRSVQT
                     ITIPVFWVHDSHDWICPFEDVKPIVDLHLNHIQFYISNGLGHNKIYRNNTVMQKITDF
                     LIH"
     gene            complement(664..1713)
                     /locus_tag="PLU36_10855"
     CDS             complement(664..1713)
                     /locus_tag="PLU36_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HMZ47294.1"
                     /translation="MKIKTALCSFGTSGKFFHAPFIYNHPGFDLYAVVERTNKQAKEK
                     YPNIISYNSFYELINDAAVDVVIINTPNITHFEYATMALHANKHVIVEKPFTTTVKEA
                     QALITLAKEKNKLLTVYHNRRYDSDFLTVQDIVIKKVLGEIHEARFCFDRYKPELSNK
                     IHKETPGVGSGILFDLGSHLIDQTIQLFGLPNAVFADIGKQRTNSKIDDYFDLKLYYK
                     KSSVSLHSSLLVKENNIGYILHGTNGSFIKPKTNIQEEFLLQNLLPTLHNFGAESEFD
                     FGVLNATINNKLVREKVPSVTGNYMQFYTSMYNAIINQAPLPVSAEEAMQVIQIIEAA
                     YISNRERRVIELSTS"
BASE COUNT          614 a          280 c          281 g          663 t
ORIGIN      
        1 taatttaaaa cttgttggtt ggcaatggaa tgcaaaaaat agtaatggga aaaaagtact
       61 tattgcacat gggtttgata gctgtagcta taaatttgat aaacttatac aacacttact
      121 gagtgcaggt tttacagtac tagcttttga tgctcctgca catggtttaa gcgatggcaa
      181 aactgtaaat gcactagact ataagaaagc gttactggca ataaacaagc aagtagaaga
      241 tttatattgt atcattggtc attcgctagg tggtttagct gcttctttag cagcagaaga
      301 gatgcatgaa ttgaagaaac ttattttaat tgcaccagca acagaaaccc aaagagcaat
      361 tgataatttt tttaagtatg tacccttagg aaaagatata aaagatgaaa tagaaaaaat
      421 aatttacaat tttgagcaaa agcctgttag ctattatgct gttagcagaa gtgttcaaac
      481 tatcaccatt cctgtatttt gggtacatga tagccatgat tggatttgcc cttttgaaga
      541 tgtaaaacct attgtagatt tacatttaaa ccatattcaa ttttatattt ccaacggatt
      601 ggggcataat aaaatttata gaaataatac agttatgcaa aagattactg actttctgat
      661 tcactaagaa gtagataatt ctatcactct cctttctctg ttgcttatat aagctgcttc
      721 tataatttga ataacttgca ttgcttcttc tgcacttact ggtaatggag cttggttaat
      781 tattgcatta tacatagatg tataaaactg catatagtta cctgtaactg atggtacttt
      841 ttctctaact aatttattat ttatagtagc atttaataca ccaaaatcaa actcactttc
      901 tgccccaaaa ttatgtaaag tgggtaacaa gttttgtaat aaaaactctt cttgaatatt
      961 ggttttaggt ttaataaatg aaccattagt gccatgtaaa atgtatccaa tgttattttc
     1021 tttaactaat aaactactat gtaagctaac actagatttt ttataataaa gttttaggtc
     1081 aaagtagtca tcaatcttgc tattggttct ttgtttacct atatcagcaa aaactgcatt
     1141 tggtagtcca aataattgaa ttgtttggtc tatcaagtga gagcctaaat caaacaaaat
     1201 accagaacct acacctggtg tttctttatg aattttattg cttaattcag gtttatacct
     1261 atcaaaacaa aatcttgctt catgtatttc gcccaatact ttttttataa caatatcttg
     1321 aacagttaaa aaatcgctat catatcttct gttatgataa acggttaata atttattttt
     1381 ttcttttgct agagttatca atgcttgggc ctcctttact gttgtagtaa atggtttttc
     1441 aactattaca tgcttattag catgcaaagc cattgtagca tattcaaaat gagtaatatt
     1501 tggtgtgtta atgataacta catcaacagc agcatcattt attaattcat aaaaactatt
     1561 gtaagaaata atgttaggat atttttcttt tgcttgcttg tttgttcttt ctacaacagc
     1621 atacaaatca aacccaggat gattataaat aaatggtgca tgaaaaaatt ttccactagt
     1681 gccaaaacta cacaaggctg ttttaatttt cataattggt atttgtttgc cattactttg
     1741 taaaccaact taataaaata cctgtagcaa ctgctgcatt taaactttct gcaccacctt
     1801 ttttaggtat tgttacagga tagttgatga ctggcaat
//
LOCUS       DAOQPK010000334         1821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_62304_length_1821_cov_3.661382, whole genome shotgun sequence.
ACCESSION   DAOQPK010000334 DAOQPK010000000
VERSION     DAOQPK010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62304_length_1821_cov_3.661382"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="PLU36_10860"
     CDS             complement(<1..1092)
                     /locus_tag="PLU36_10860"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HMZ47295.1"
                     /translation="MANRLKKKNIETPNPEELTTDKVADVTVAEVVKDERTTKIMGAV
                     SLLFTIFLFVAFTSYLFTWQEDQDKVQKFGVKIFSISDVQVSNLLGLLGAYIAHVFIY
                     KGFGIASFLFCTFFFVLGVNLLFGKKIFSIKRNIKYLITGLIVLSVAFTFMAAKAEFS
                     WGGAVGELLNSWLIKWIGKIGTGTILLLSVFAYFIWRFNPTFTYPEQFFKNIFAPKPK
                     KEIPTEDVAETQDNETINKLTVDSNDEEELQNFELKENINKEPTSNNRLKTEGNAVTV
                     IMPDVEENNLPIFDIVDKPEIELNEVLVAEAPLSVNDKFANAENSNHDLSLEIKPITD
                     ENNSVEEQEIETTNKIEEELDEVDIDEQEE"
     gene            complement(1239..>1821)
                     /locus_tag="PLU36_10865"
     CDS             complement(1239..>1821)
                     /locus_tag="PLU36_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018614962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HMZ47296.1"
                     /translation="YTEFEGKYSQNFNVKKDLLKIGVINQTTQLATDTQAIADYLKMV
                     MIKKFNLDAKSVSNHFADTRDTLCYATNDNQSAVSGMLNTPADLALVIGGYNSSNTSH
                     LVELCEQKLPTYFIESAAKIISKSELIDCNWKTKEIKTVQHYLPQTTPVKILITSGAS
                     CPDALVEEVIRKIASFYNAEEKIEEMEKIFSVN"
BASE COUNT          576 a          311 c          246 g          688 t
ORIGIN      
        1 ttcttcttgt tcatcaatat ccacttcatc caattcttcc tctattttat ttgttgtttc
       61 aatttcttgt tcttcaacac tattattttc atcagttatt ggttttattt ctaaacttaa
      121 atcatggtta ctgttttctg catttgcaaa cttatcattt actgatagcg gagcttctgc
      181 cactaaaact tcgttcaatt caatttctgg tttatctaca atatcaaaaa ttggcaaatt
      241 attttcttcc acatctggca taattacagt tactgcatta ccttctgttt ttaatctgtt
      301 attgctggtt ggctctttat ttatattttc ttttagttca aaattttgca attcttcttc
      361 atcattacta tcaacagtaa gtttgtttat tgtttcatta tcttgtgttt cagcaacatc
      421 ttctgtagga atttcttttt taggctttgg agcaaaaata tttttaaaga attgctcggg
      481 gtatgtaaat gttggattga aacgccaaat aaaatatgca aaaacactta gtagcaatat
      541 tgtgcctgtt ccaatttttc caatccattt aattaaccaa ctatttaata actctccaac
      601 agcaccaccc cagctaaact cagctttagc tgccataaaa gtaaaggcaa cacttaaaac
      661 tattaaacct gtaattaagt atttaatatt tcttttaatt gaaaatattt tttttccgaa
      721 aagtagattt actcctaata caaaaaagaa agtgcaaaat aaaaacgatg caatgccaaa
      781 gcctttgtaa ataaaaacat gagcaatata agcacccaat aaccctaata aattactaac
      841 ctgaacatca gatattgaga aaatttttac gccaaatttt tgtactttat cttgatcttc
      901 ttgccaggta aataaataag atgtaaaagc aacaaataaa aaaatagtaa acaataaact
      961 aacagcaccc attattttag tagtacgttc atcttttaca acttcggcta ctgtaacatc
     1021 tgcaacctta tctgttgtta actcttcggg gttgggagtt tcaatatttt tcttttttaa
     1081 acgattggcc ataatgtagg caaattaaat attgcctaag ctacaaatgc atctacagtt
     1141 ataacaacca tgtggattac ttttggttaa aaactacatt aaatgcccaa agtggataac
     1201 ttatggaatt tttttattgc aaaaaggaag aaggaatact agttaactga aaatattttt
     1261 tccatttcct ctattttttc ttcggcatta taaaagctgg caatttttct gattacttct
     1321 tcaaccaatg catcggggca gcttgcacca cttgttatta aaatttttac tggtgtagtt
     1381 tgtggtaaat aatgctgcac agttttaatt tctttggttt tccagttgca atcaatcagt
     1441 tctgatttgc tgataatttt tgcagcagat tcaataaaat aagttggtaa tttttgttca
     1501 caaagttcca ctaaatggga agtgtttgat gaattgtagc caccaattac caatgccaaa
     1561 tctgcaggtg tatttaacat accagataca gcagattgat tatcgtttgt ggcataacaa
     1621 agcgtatctc ttgtatctgc aaaatgatta gacaccgatt tagcatcgag gttaaacttt
     1681 tttatcatta ccatttttaa ataatctgca atggcttgtg tatctgtagc taattgtgtt
     1741 gtttgattga ttaccccaat tttcaataaa tcttttttaa cattaaagtt ttggctgtat
     1801 ttgccttcaa actcagtata a
//
LOCUS       DAOQPK010000335         1790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_64469_length_1790_cov_4.473199, whole genome shotgun sequence.
ACCESSION   DAOQPK010000335 DAOQPK010000000
VERSION     DAOQPK010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64469_length_1790_cov_4.473199"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1790
                     /locus_tag="PLU36_10870"
     CDS             <1..>1790
                     /locus_tag="PLU36_10870"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMZ47297.1"
                     /translation="DAAFVLALNPSNNNIYFAGGTASTDLLGDKTNVLFSSNQGGGSN
                     VDGFVSILKNDGSQFIKTTYMGTNGIDIVFGIQFDKFSYPYIMGITTGNWPVIAANYS
                     QTNGKQFISKLQPDLSGFIFSTKFGTNQSSPNISPVAFLVDRCENIYVSGWGGGINGD
                     PSANYVPNQSTVGLPVTADAIKSNSDGSDLYFFVLEKNAQSLLYGSFFGQNDNAASIG
                     EHVDGGTSRFDRNGVIYQSVCANCGGGATFPTTPGTWSPNNGTGGQRCNLAAIKIAFN
                     FAGVGSSVQSSVNGVKDTSGCVPLTVTFKDTIANATKYIWSFGDGSPDATTTTPIVQH
                     LYTAIGDYNVRLVSIDSNTCNIADTSYTILRVRNDDAFPSFTVTRLLPCTSLNYSFTN
                     TSNFPSGKPFKSNSFTWDFGDGTTQTAGGQTVTHSYAAAGTYNVKLILNDTNYCNSPD
                     TAFESLKVAPLVVAKFETNTAGCIPYNATFKNTSTAGTDFIWDFGDGSPISTATNPTH
                     NYTTAGNFTIKLKAIDNGTCNKIDSTSFTITVSPNPNSSFDFNPKPPQINTPVTFINN
                     SVGGNKYSWHFGDGDSLVTNNINASVLHQY"
BASE COUNT          551 a          296 c          323 g          620 t
ORIGIN      
        1 atgatgctgc ttttgtatta gctcttaatc catctaataa caatatttat tttgcaggag
       61 gtactgcaag tacagatttg ctaggcgata aaaccaatgt actttttagt tctaatcaag
      121 gtggtggcag taatgtggat ggctttgttt ctattttaaa aaatgatggc tcacagttta
      181 ttaaaacaac ttatatgggt accaatggaa ttgatatagt ttttggtatt cagtttgata
      241 agtttagcta cccttatata atgggtatta caacgggcaa ctggcctgta attgcagcaa
      301 actatagcca aacaaatggt aagcaattta tttcaaaact tcaacccgat ttatctggtt
      361 ttattttttc aacaaaattt ggtactaatc aaagtagtcc taatatttct cctgttgctt
      421 ttttagtgga tcggtgtgaa aatatttatg tttctggctg gggtggtggc attaatggag
      481 acccttctgc caattatgta cctaaccaat caactgtagg tttacctgta actgcagatg
      541 ctataaaatc taacagcgat ggtagcgatt tatacttttt tgtgttagaa aaaaatgcac
      601 aaagtttatt atatggtagt ttttttggac aaaatgataa tgctgcttct attggcgaac
      661 atgtagatgg tggaacgagc aggtttgata gaaatggcgt aatttatcaa tcggtttgtg
      721 ccaattgtgg cggtggtgct acttttccta ctacacctgg tacttggtct cctaacaatg
      781 gtacaggtgg gcaacgttgt aatttagctg ccattaaaat agcatttaat tttgctggtg
      841 taggcagttc tgttcaatca tctgttaatg gtgttaaaga tacttctggt tgtgtgcctt
      901 taacagtaac ttttaaagat acaattgcta atgccactaa atatatttgg agttttggag
      961 atggcagccc agatgctaca actaccacac caattgtgca gcatttatat acagctattg
     1021 gagattataa tgttagattg gtttctatag attctaatac ttgcaatatt gcagacacat
     1081 cttatacaat tttaagagta agaaatgatg atgcctttcc tagctttaca gtaacccgtt
     1141 tactaccctg tacaagttta aattatagtt ttaccaatac ttccaacttt ccaagtggaa
     1201 agccttttaa aagtaatagt tttacttggg attttggtga tggtacaaca caaacagcag
     1261 gtgggcaaac agtaacgcat tcttatgctg ccgctggtac ttataatgta aagctaattt
     1321 taaacgatac taactattgt aattctccag atactgcctt tgaaagttta aaagttgcac
     1381 ctttagttgt agcaaagttt gaaacaaaca ctgcaggttg tatcccttac aatgcaacat
     1441 ttaaaaacac atcaacagct ggaactgatt ttatttggga ttttggtgat ggctcaccta
     1501 tttcaactgc tacaaatcca acgcataatt atactactgc tggtaatttt acaattaaat
     1561 taaaagccat agataatggt acttgtaata aaatagatag cacttcattt actataactg
     1621 ttagcccaaa tcctaattca tccttcgatt ttaatcctaa acctcctcaa ataaatacac
     1681 cagttacgtt tatcaataat tctgttggtg gcaataaata ctcttggcat tttggcgatg
     1741 gagatagctt ggttactaat aatataaacg ctagtgtttt acaccaatat
//
LOCUS       DAOQPK010000336         1777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_65320_length_1777_cov_5.112660, whole genome shotgun sequence.
ACCESSION   DAOQPK010000336 DAOQPK010000000
VERSION     DAOQPK010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65320_length_1777_cov_5.112660"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..1326)
                     /locus_tag="PLU36_10875"
     CDS             complement(169..1326)
                     /locus_tag="PLU36_10875"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018612764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HMZ47298.1"
                     /translation="MSVKRIGIFGSTGSIGTQALEVIAANKNLFCASILTAQNNHELL
                     IKQAVEFKPDVVVIGDEKKYALVKEALANTNVKVMAGEHAIEEAAGMDCYDMMLAAIV
                     GYAGLKPTLKAIDAAKPIALANKETLVVAGEIVMQKAVEKNVGIIPVDSEHSAIFQCL
                     VGEGKNKIEKIILTASGGPFRGKKPNFLVNVKRDHALQHPNWSMGAKITIDSATLMNK
                     GLEMIEAKWLFNLKPEQIQVVIHPQSIIHSMIMFEDGSIKAQMGLPDMKLPIQYAMAF
                     PYRIENNFPRYDFRKPDTLNFEEPDVKTFRNLILATNALVQGGNMPCILNAANEIAVF
                     AFLRNRIGFLDITEMVERTINKIPFIANPTLEELFVSDGEARNFAASLVEL"
     gene            1488..1560
                     /locus_tag="PLU36_10880"
     tRNA            1488..1560
                     /locus_tag="PLU36_10880"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1521..1523,aa:Ala,seq:ggc)
BASE COUNT          558 a          303 c          281 g          635 t
ORIGIN      
        1 acacaactaa aattgaaaac gataatacaa actgccctgc ttgtattaaa ccaccattcc
       61 aatcaactgc tatcaattcc ataattattt aaacttttat tttatatttt gttattgtaa
      121 aaaatatttt ttttgagaaa atcttttgta gcaatagttt taaaaatttt acaattctac
      181 taaagaggct gcaaaattac gtgcctcacc atcgctaaca aacaattctt ctaacgtggg
      241 attagcaata aagggtattt tgttaatagt tctttctacc atttcggtaa tatctaaaaa
      301 gccaattctg ttgcgtaaga aggcaaatac agctatttca ttggctgcgt ttaaaataca
      361 tggcatgttg ccaccttgta ctaaagcatt agttgccagt attaaatttc tgaaagtttt
      421 aacatctggc tcttcaaaat ttagggtatc tggtttgcga aaatcgtaac gaggaaaatt
      481 attttctatt ctataaggaa aagccattgc atattgaata ggcaatttca tatcgggtaa
      541 gcccatttgt gcttttatac tgccatcttc aaacattatc atactatgaa taatactttg
      601 tggatgaata actacttgta tttgctctgg ctttaaatta aataaccact tggcttctat
      661 catttcaagc cctttattca taagtgttgc agaatctatt gtaattttag cacccattga
      721 ccagttaggg tgttgtaatg catggtctcg ttttacatta actaaaaaat taggtttttt
      781 gcctctaaat gggccaccac ttgcagttaa aataattttt tctattttat tttttccctc
      841 acctaccaag cattgaaata tggcagaatg ttcactgtca acaggaataa tgcctacatt
      901 tttttctact gctttttgca ttactatttc tccggcaact actaaggttt ctttattggc
      961 caatgcaata ggtttggctg catctattgc ctttaatgtt ggttttaatc cagcatatcc
     1021 aacaatagct gccaacatca tatcataaca atccataccg gctgcttctt caattgcatg
     1081 ttcgcctgcc ataactttta cattagtatt ggctaaagct tctttaacga gagcgtattt
     1141 tttttcatcg ccaattacaa ctacatctgg tttaaactct actgcttgtt ttattaataa
     1201 ttcatgatta ttttgagctg taagtatgga tgcacaaaat aaatttttat tggctgcaat
     1261 tacttctaat gcttgcgtac caattgaacc tgttgaacca aatatgccta tacgtttaac
     1321 gctcattttt tttgcttttg gcagaagcgt aaagataggg ctaataggaa aatttgaaac
     1381 attaaattaa atcaaaattt ttcttgtgaa tattttagtt ggctaaaaaa aataataatc
     1441 tacatttgta aacttcgcca taaatacctt tactttgtca accttttggg ggtttagctc
     1501 agttggtaga gcgtttgcat ggcatgcaaa aggtcagcgg ttcgaccccg ctaatctcca
     1561 ctaaataagt tcataaaatt ttttttgttt ttaaataagg attgcaagtt gcaactacgt
     1621 atttgctgag tagatgaaag tggaaggtaa acaagtatga acgaattaaa atgtagagtt
     1681 atcgtgtgta atagttagat taagggcata aaaaaagccc gaagtatcag gcattactgc
     1741 ttctaaagaa ctgttgctgt gtaaaagtag gagaaaa
//
LOCUS       DAOQPK010000337         1761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_66456_length_1761_cov_5.948417, whole genome shotgun sequence.
ACCESSION   DAOQPK010000337 DAOQPK010000000
VERSION     DAOQPK010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66456_length_1761_cov_5.948417"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PLU36_10885"
     CDS             complement(<1..353)
                     /locus_tag="PLU36_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47299.1"
                     /translation="MMQSIKTIVIILFVLFCYSVSAQTSYSLLGHKEYNILDRLEIKS
                     KLPAFGASFIKPYNIRLLVKEVETIDSLQRNNNRIVASLSTVDKYNITRFLMQHKEYT
                     SLTSSFNNKKSAFNL"
     gene            388..1731
                     /locus_tag="PLU36_10890"
     CDS             388..1731
                     /locus_tag="PLU36_10890"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008582325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HMZ47300.1"
                     /translation="MNHYLIKDTHIVNEGKIVSGDVLIKNGIIEKVGGVINTKNNPIE
                     VDGSNQHLLPGIIDDQVHFREPGLTHKATIYSESKAAVAGGVTSFMEMPNTSPPTFTQ
                     ALLEEKYAIAKQTSLANYSFFMGTGNDNYEEVMKTNEKKNEVCGIKIFMGSSTGNLLV
                     DNPILLDKIFANAELLIATHCEEESIIKNNLEKLIATKTNLEAADHAVIRNEEACFEC
                     SFKAIQLAHKHNTRLHILHISTQKELQLFGNIVPLKEKRITAEVCVHHLHFTANDYAI
                     LGNKIKCNPAIKAAYNKEALWQALLNDKLDIIATDHAPHTIEEKNGTYLKAHAGLPLV
                     QHSLMLMLHYVQQGKITLEKVVEKMCHSVADCFTIKNRGYIKEGYFADLVLVNCYQPY
                     TINKENILYKCTWSPLEGFTFPATITHTFVNGNVVYSNGVFKEEFKGSRILFDRK"
BASE COUNT          682 a          251 c          275 g          553 t
ORIGIN      
        1 attaaattga aagcagattt tttattattg aaggaagagg ttaaagaagt gtattcctta
       61 tgctgcatta aaaaacgagt aatattgtat ttatcaacag tagataaact ggccacaatt
      121 ctgttattgt tcctttgtaa actatctatt gtttctactt cttttaccaa taaccttata
      181 ttatatggct taataaagga agctccaaaa gcaggcaatt tagactttat ctctaaccta
      241 tctaatatat tatattcttt atgccccaat agagaatagc ttgtttgtgc cgaaacacta
      301 taacaaaata aaacaaacag aattataaca atagttttta tactttgcat cataaatatt
      361 gaaaaattgt tgtcaaaaat aaatgctatg aatcattatt taattaaaga tacacatata
      421 gtaaacgaag gcaagattgt tagtggtgat gtgttgataa aaaatggaat tatagaaaaa
      481 gttggaggag ttattaacac caaaaataac cccattgaag tggatggaag taaccaacat
      541 ttattacctg gtattattga tgatcaggta cattttagag agccaggtct tacacataaa
      601 gcaactattt attccgaatc taaagcagca gttgcaggtg gcgtaaccag ttttatggaa
      661 atgcctaaca cctcgcctcc tacttttaca caagctttgc tagaagaaaa atatgcaatt
      721 gcaaaacaaa catctttagc taattattca ttttttatgg gtacaggaaa tgataattat
      781 gaagaagtaa tgaaaacaaa tgagaaaaag aatgaggtat gtggtataaa aatttttatg
      841 ggtagtagta ctggaaattt attagtagat aacccaattt tattagataa aatttttgca
      901 aatgcagagc tgcttattgc tacacattgc gaagaagaat caattattaa aaacaatctg
      961 gaaaaattaa tagcaacaaa aaccaactta gaagcagcag accatgcagt tattagaaat
     1021 gaagaagctt gttttgaatg ttcatttaaa gcaatacaac ttgcacacaa acacaatacc
     1081 aggttacata ttttacacat cagtacccaa aaagaacttc agctttttgg aaatattgta
     1141 ccattaaaag aaaaaagaat tactgccgaa gtatgtgtac atcatttaca ctttactgct
     1201 aatgattatg caattttagg taataaaata aaatgcaacc cagccattaa agctgcatac
     1261 aacaaagaag cactatggca agccttatta aatgataaac tagatattat tgctaccgac
     1321 catgccccac atactattga agaaaaaaat ggaacatact taaaagccca tgctggctta
     1381 cctttagtac aacattcttt aatgttgatg ttacattatg tacaacaagg taaaattact
     1441 ttagaaaaag tagttgaaaa aatgtgccac tcagtagcag attgttttac tataaaaaac
     1501 agaggctata ttaaagaagg ttattttgcc gatttggttt tagtaaattg ctaccaacct
     1561 tataccataa acaaagaaaa tattttatac aaatgtacat ggagcccatt agaaggtttt
     1621 acttttcctg caacaattac acatactttt gttaatggta atgtagtgta tagtaatggt
     1681 gtttttaagg aagagtttaa aggaagtaga atattgtttg atagaaaata attagctcat
     1741 attttataca ttgataatgc a
//
LOCUS       DAOQPK010000338         1755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_66894_length_1755_cov_4.127059, whole genome shotgun sequence.
ACCESSION   DAOQPK010000338 DAOQPK010000000
VERSION     DAOQPK010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66894_length_1755_cov_4.127059"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="PLU36_10895"
     CDS             complement(<1..980)
                     /locus_tag="PLU36_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HMZ47301.1"
                     /translation="MFNNLSEKLEIAFKNLKGQSRINDLNIANTIKDIRRALLDADVN
                     FKIAKEFTDKVKVEAIGSKVLHAVSPGQQMVKIVQDELTALMGGEPAEFNISGNPAII
                     LIAGLQGSGKTTFTGKLATYLKDKKGKSPMVVAADIYRPAAIDQLTVLAQQIGVDIYK
                     EPETKDAVLIAQNAIKEAKAKNKNVVIIDTAGRLAIDEIMMTEVANIKAAVNPSEILF
                     VVDSMTGQDAVNTAKAFNDKLDFSGVVLTKLDGDTRGGAALSIKYTVNKPIKFMSSGE
                     KMDTLDVFYPDRMAQRILGMGDITTLVEKAQQQFDEEQAKKLEKKIRKNQ"
     gene            1252..1635
                     /locus_tag="PLU36_10900"
     CDS             1252..1635
                     /locus_tag="PLU36_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HMZ47302.1"
                     /translation="MSKKQLFTLEFKVRCSPSILFEFLSTPTGLQEWFADKVDEWEGE
                     LSFSWSGGTPDKAVVIDAEQDKFIRYRWLHAPKEEYFEFKIAKTEISNQTILVISDFA
                     EKSEIKDQTQLWDYQVKELLHRLGS"
BASE COUNT          569 a          291 c          235 g          660 t
ORIGIN      
        1 aattgatttt ttcttatttt tttctctaat tttttagctt gctcttcatc aaattgttgt
       61 tgtgcttttt ctactaaggt ggtaatatct cccattccta aaatacgctg agccattcta
      121 tctggataga aaacatctaa agtatccatc ttttctccac tgctcataaa ctttatgggt
      181 ttatttactg tgtattttat agaaagagca gcaccacctc tggtatcacc atctaattta
      241 gttaaaacca caccactaaa atctagttta tcattaaaag cttttgcagt gtttacagca
      301 tcttgccctg tcatgctatc tacaacaaat aaaatttctg aagggtttac agctgcttta
      361 atattggcaa cttcggtcat cattatttca tctattgcaa gcctacctgc agtatctatt
      421 ataacaacat ttttattttt tgcctttgct tctttaattg cattttgtgc aattaaaact
      481 gcatcttttg tttcgggttc tttataaata tctacaccaa tttgttgtgc tagtacagtt
      541 aactggtcta tagcagcagg tctataaata tctgcagcaa ccaccattgg cgattttcct
      601 tttttatctt ttaaataagt agccagtttt cctgtaaaag ttgttttacc actaccttgt
      661 aagccagcta ttaaaataat agcagggtta ccactaatat taaactctgc aggttcgcca
      721 cccattaatg cagttaattc atcttgaaca atttttacca tttgttgccc aggactaaca
      781 gcatggagca ctttactacc aatagcttct acctttactt tatctgtaaa ttctttggca
      841 attttaaaat tcacatctgc atctaataag gcacgcctaa tatctttaat agtattggct
      901 atatttaaat catttattct agactggcct ttaagatttt taaatgctat ttctaatttt
      961 tcagataaat tattaaacat tatattttat ttttattact ctttgatgta aaataaggct
     1021 gcaaatataa tagcactaaa gttgttgccc taaatacctt tttatctttt aataaattgt
     1081 actaattagc tttcatctta attatcaatt atttacttaa cctaaattgt atgaattatt
     1141 tttttatata gaattctgaa taaagaatta aaataaaatt ataaaaacat ttgtttaaac
     1201 tgtattaatt gctattattg cagcacttga taaaggttta aagacttact aatgagtaaa
     1261 aaacaactat ttacacttga gtttaaagtt agatgttcac catcaatttt atttgaattc
     1321 ttaagcactc caaccggttt gcaagaatgg tttgcagata aagtagatga gtgggaaggg
     1381 gaattgagtt ttagttggag tggtggcaca ccagataagg cagttgtaat agatgcagag
     1441 caagataaat ttattagata ccgatggtta catgcaccaa aagaagaata ttttgaattt
     1501 aaaattgcca aaacagaaat ttcaaaccaa actattttag taatttcaga ttttgccgaa
     1561 aagtccgaaa taaaagatca aacgcagcta tgggattatc aggtaaaaga attattacat
     1621 agattaggaa gttaaaaact atctcttcta caaattaatc actaaaaaga ttctctcaca
     1681 cttcacaaat tttttctagc ttttttacac ttttgtacat tattgttata gagtttctgc
     1741 tacataaata agtac
//
LOCUS       DAOQPK010000339         1688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_71986_length_1688_cov_3.650949, whole genome shotgun sequence.
ACCESSION   DAOQPK010000339 DAOQPK010000000
VERSION     DAOQPK010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71986_length_1688_cov_3.650949"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1613)
                     /locus_tag="PLU36_10905"
     CDS             complement(<1..1613)
                     /locus_tag="PLU36_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47303.1"
                     /translation="MQISSQIFNPFPGLRSFEENEDLLFFGREKQVTELVKKLRQEKF
                     LAVIGSSGSGKSSLVKSGLIPALHSGFMSGAGSSWKICTFRPGNNPIGNLATALSSDN
                     LLYHNIETTEDSFTYTAIIESTLRRSSQGLVEAYKQSRIDKKNNLLILVDQFEEIFRF
                     SRVEKDAKEGKRDSIAFINLLLKASEQKEFPIYVVFTMRSDFLGDCTEFRGLPEAINE
                     GQYLVPRMTRDERREAITGPVAVGGATITPQLLNQLLNDVGDNPDQLPILQHALMRTW
                     NIWSSKSNNNTPIDIDAYEEIGTMSNALSQHAEEAYNDLNTDRKKQICESLFKSLTDR
                     GSDTRGIRRPTKLSEICKLANASSNEVIEVIDVFRANGRSFLMPPSEVAINEDTIIDI
                     SHESLMRGWNRLLNWVEEENQSAEIYLRLCEAANLYELGKGGLWRDPELQLAWKWKEE
                     AKPNVTWASRYNNYFEKAILFLDYCKQQSELAIQHKEKMHKRRLKRARLIAIFISCIA
                     IAALLLAIFSFDQRNKANQQKIIALNKTE"
BASE COUNT          486 a          314 c          254 g          634 t
ORIGIN      
        1 tcctctgttt tatttaatgc aattattttt tgttggttag ctttattacg ttggtcaaaa
       61 gaaaatattg ccaataacaa agcagctatg gcaatacagg aaataaaaat agcaatcagc
      121 cttgcccttt ttaaccttct tttgtgcatt ttttctttat gctgaatggc taattcgctt
      181 tgttgtttac aataatctaa aaaaagaatt gctttttcga agtagttatt gtaacgagaa
      241 gcccatgtta catttggttt agcttcttct ttccatttcc aggcaagttg taattctggg
      301 tctctccata agcctccttt tcccagttca tacaaattag cagcctcaca aagacgtaaa
      361 taaatttcgg cagattggtt ttcttcttct acccaattta ataatctgtt ccaacctctc
      421 attaaacttt cgtgagaaat atcaatgatg gtatcttcgt taattgctac ttcgcttggt
      481 ggcattaaaa agcttctacc atttgctcta aaaacatcaa ttacttctat cacttcgtta
      541 gagctggcat tggcaagttt acatatttct gatagtttgg ttggccttct tatacctctt
      601 gtatcgctac ctctatctgt taatgattta aaaaggcttt cgcaaatttg tttttttcta
      661 tctgtgttta aatcgttata tgcttcttct gcatgttgag ataaagcatt agacattgtt
      721 cctatttctt cataagcatc aatatcaata ggcgtattat tattactttt ggaagaccaa
      781 atattccatg ttcgcattaa tgcatgttgc aaaatgggta attggtctgg gttatcgcca
      841 acatcattta acagttgatt taatagttga ggggttatag ttgccccacc aacagctact
      901 ggtcctgtaa ttgcttctct tctttcgtct cttgtcattc taggtacaag atattgccct
      961 tcatttatcg cttctggcaa tcctctaaac tcggtacaat ctcctaaaaa gtcagaccgc
     1021 atggtaaata caacataaat aggaaattct ttttgttcgg atgcttttaa aagtaaatta
     1081 ataaatgcaa tggagtctct ttttccttct ttagcatctt tctcaaccct gctaaatcta
     1141 aaaatttctt caaattgatc taccagtatt agcaggttat tttttttatc aattctactt
     1201 tgtttatagg cttctaccaa gccttggctg cttcttctta aagtgctttc aataatagca
     1261 gtataagtaa agctgtcttc ggttgtttca atattatgat ataataagtt atcagaagaa
     1321 agggcagtag ctaaattgcc aattgggtta ttgcctggcc tgaaagtaca aattttccaa
     1381 ctagagcctg caccagacat aaagccacta tgcaatgcag gaattaatcc tgattttact
     1441 aaggaagatt ttccactacc cgatgaacct ataactgcta aaaatttttc ttgcctcagt
     1501 tttttaacca attctgttac ttgcttttct cttccaaaaa acaataagtc ttcattttct
     1561 tcaaatgaac gaaggcctgg aaatgggtta aaaatttggc tacttatttg catctgtatt
     1621 ggcaattaaa atatcattta ttcaacaaaa tgaataattt gttttataac ttttagatgt
     1681 acaatatt
//
LOCUS       DAOQPK010000340         1684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_72312_length_1684_cov_4.106814, whole genome shotgun sequence.
ACCESSION   DAOQPK010000340 DAOQPK010000000
VERSION     DAOQPK010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72312_length_1684_cov_4.106814"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PLU36_10910"
     CDS             complement(<1..479)
                     /locus_tag="PLU36_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HMZ47304.1"
                     /translation="MGKTKKPKHIAVAGNIGAGKTTLTEMLSKHYRWIPQFEDVDHNP
                     YLMDFYEDMPRWSFNLQIYFLNSRLNQLLDIQRGTETIIQDRTIYEDAHIFAPNLHDM
                     GLMSKRDFENYFRFFQTLKTMVQPPDLLIYLKASVPTLVGQIEKRGREYEENIRLDY"
     gene            complement(578..1582)
                     /gene="trpS"
                     /locus_tag="PLU36_10915"
     CDS             complement(578..1582)
                     /gene="trpS"
                     /locus_tag="PLU36_10915"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HMZ47305.1"
                     /translation="MNKEIVLSGIRPTGFLHLGNYFGAMRNYIKMQEDYNCYFFVANW
                     HSLTTHPDTKELKNAVRRVIAENIACGLNTDKAVLYVQSDVPEIAELYLYLNMLAYKG
                     ELEKTTTFKDKARSQPDNVNAGLLTYPVLQAADILIHRAVKVPVGKDQEQNLEMARNF
                     AQRFNHRYGDIFPMPFAFNYGNELVKILSLDGNGKMSKSENQLATLYLADDDDTIRKK
                     ILKAKTDSGPTETNAIKPDYIQNLFTLMSFASSEDTVQKFEEDFNKSSSGNCIIRYGD
                     MKKQLAEDMINFIKPIREKAADLQQNTDLLNKIIKQGADKARDSASQTLQQVRKAVLG
                     "
BASE COUNT          539 a          267 c          224 g          654 t
ORIGIN      
        1 aaataatcca gtcttatatt ttcttcatat tctctaccac gtttttcaat ttgtcctaca
       61 agtgttggta cagatgcctt taaataaatt aataaatctg gtggttgaac catggttttt
      121 aacgtttgaa agaatctgaa ataattttca aaatctcttt tactcattaa acccatgtcg
      181 tgtaagttag gagcaaaaat gtgtgcatct tcataaatcg tcctatcttg aataatggtt
      241 tctgtacctc tttgtatatc taataattga tttaagcgac tatttaaaaa atatatttga
      301 agattaaaac tccagcgtgg catatcttca taaaaatcca ttaaatacgg attatgatct
      361 acatcttcaa attgtggtat ccaacgataa tgtttactta acatttctgt aagtgttgtt
      421 ttacctgcac caatattacc tgcaacagca atatgttttg gtttttttgt tttacccata
      481 atattttata ttctaaaaaa aattattcca tttaaagtcg tgaaagtaag atttatcctt
      541 tataatttca caaataaata gcatcaaaaa attatattta acccaaaacg gcttttctaa
      601 cttgttgtaa tgtttgcgat gcactatctc ttgctttatc tgctccttgt ttaattattt
      661 tatttaataa atctgtattc tgttgtaagt ctgcagcttt ttctctaatt ggttttataa
      721 agtttatcat atcttctgct aattgttttt tcatatcgcc gtatcttatt atacaattac
      781 cagagctgct tttattaaaa tcttcttcaa acttttgtac agtatcttct gaactggcaa
      841 aagacattaa ggtaaatagg ttttgtatat aatctggttt tattgcattg gtttctgttg
      901 gtccactatc ggttttagct ttaagtattt tttttctaat ggtatcatca tcatctgcta
      961 aatataaagt agcaagttgg ttttcacttt tactcatttt accatttcca tcaaggctta
     1021 aaatttttac aagctcatta ccataattaa aagcaaatgg cataggaaaa atatctccat
     1081 aacgatgatt aaaacgctga gcaaaatttc ttgccatttc taaattttgc tcctgatcct
     1141 taccaacagg tactttaaca gctctgtgaa ttaaaatatc tgcagcctgt aatacagggt
     1201 atgtaagcaa tcctgcatta acattatctg gttgagaacg agctttatct ttaaaggttg
     1261 tagttttttc taattcacct ttataagcta acatattcaa ataaaggtat agctcggcaa
     1321 tttcgggtac atcgctttgt acatataaaa ctgctttatc tgtattaagt ccgcaagcta
     1381 tattttctgc aataactctt cttactgcat ttttaagctc ctttgtgtcg ggatgtgtgg
     1441 ttaatgaatg ccagttagca acaaagaaat agcaattata atcttcttgc attttaatat
     1501 aattacgcat ggctccaaaa taattaccta aatgtaaaaa tccggttggt ctaattccgc
     1561 ttaaaacaat ttctttattc ataatggcaa aaatagttgt ttaagaataa gatgttaaat
     1621 tgaaatgtat tttaaaacga ataaattaaa agataaaaac atttaaaaat atagtttcta
     1681 tttt
//
LOCUS       DAOQPK010000341         1663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_74111_length_1663_cov_4.200871, whole genome shotgun sequence.
ACCESSION   DAOQPK010000341 DAOQPK010000000
VERSION     DAOQPK010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74111_length_1663_cov_4.200871"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1347
                     /locus_tag="PLU36_10920"
     CDS             <1..1347
                     /locus_tag="PLU36_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018616311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HMZ47306.1"
                     /translation="HNVLNAKQHAREAQIVAEAGLAGAVTIATNMAGRGTDIKLGPNV
                     KEAGGLAIIGTERHESRRVDRQLRGRAGRQGDPGSSQFFVALEDDLMRMFGSERIAKV
                     MDFAGYKEGEVIQHSMITKSIERAQRKVEENNFGIRKRLLEYDDVMNKQRTVIYTKRH
                     HALFGKRLALDIDNAFYSVAKGLINNFKAINDFEGFKLAVIVNFGIDTSINIEELNKA
                     NDNSLTEKLYSEAINNYQRKKQEIALKSVPVFKNIRLHQGNNIENVVVPFSDGKKTLQ
                     VLASMQKTLDSEGVELLDSLERNLALALIDEAWKEHLRAMDDLKQSVQTATYEQKDPL
                     VIYKMEAYNLFENMNAEVNKNIVEFLCHCGIPSDEEGQQLKEGRQQKTDLSKTSNNKD
                     DIDSRGDSYGANENDYYDPSANVKQQPVIAAPKIGRNDLCPCGSGKKYKACHGKDA"
     gene            complement(1434..>1663)
                     /locus_tag="PLU36_10925"
     CDS             complement(1434..>1663)
                     /locus_tag="PLU36_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014217230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47307.1"
                     /translation="GKLDKVDNPLKNAPHTQSIVCANEWNHQYTRQQAAFPLFYVSQN
                     KFWPSVARVNNTHGDRNLICTCEPVESYVEA"
BASE COUNT          627 a          238 c          326 g          472 t
ORIGIN      
        1 cataatgtat taaacgctaa gcaacatgcc agagaagctc aaattgttgc tgaagcaggt
       61 ttagcaggtg cagtaacaat tgctaccaat atggctggtc gtggtacaga tattaaactt
      121 gggcctaatg ttaaagaagc tggtgggttg gcaattatag gcacagaaag acatgaatcc
      181 agaagggtag atagacagct tcgtggacgt gcaggccgtc aaggagatcc aggttcttct
      241 caattttttg ttgcgttaga agatgattta atgagaatgt ttggcagcga aagaattgca
      301 aaagtgatgg attttgcagg atataaggaa ggagaggtta tacagcatag tatgattaca
      361 aaaagtattg aacgagctca aagaaaagtt gaagaaaaca actttggtat cagaaaaagg
      421 ctattagagt acgatgatgt aatgaataaa caaagaactg ttatttatac caaacgtcat
      481 catgcattat ttggtaaaag attagcctta gatattgata atgcttttta cagtgtggca
      541 aaaggtttaa ttaataattt taaagctatt aatgattttg aaggttttaa attagcggtt
      601 attgtaaatt ttggtataga tacaagcatc aatattgaag aacttaacaa agccaacgat
      661 aattcattaa cagaaaagtt atattcagaa gcaataaata attaccaaag aaaaaaacaa
      721 gaaatagcct taaaatcggt tcctgtattt aaaaatattc gtttacacca aggtaacaat
      781 attgaaaatg tggtagtacc ttttagcgat ggtaaaaaaa ccttacaggt acttgcatct
      841 atgcaaaaaa cattagactc agaaggtgta gaacttttag actctttaga aagaaacctt
      901 gcattagcac ttatagatga agcgtggaaa gaacatttaa gagctatgga tgatttaaag
      961 caaagtgtac aaacggcaac ttacgaacaa aaagacccat tggttattta caaaatggag
     1021 gcatacaatt tatttgaaaa catgaatgcc gaagtaaata aaaatattgt ggagtttttg
     1081 tgtcattgtg gtattccatc agatgaagaa gggcagcaac taaaagaagg cagacagcaa
     1141 aaaacagatt taagcaaaac aagcaataac aaagatgata ttgatagcag gggagatagc
     1201 tatggagcaa atgaaaacga ttattacgat ccatctgcca atgttaaaca acaacctgta
     1261 attgctgcac ccaaaatagg cagaaatgac ctttgccctt gtggtagtgg taaaaagtac
     1321 aaagcctgtc atggtaaaga tgcataaacc atcagcctaa aaaaaatcct cattctgata
     1381 aagaatgagg atttttttat atcctaaatt gaaaaggagt gtactaaaat tttttaggct
     1441 tctacataac tctctacagg ctcacaagtg caaattaaat ttctatcacc atgtgtatta
     1501 tttacccttg ctacacttgg ccaaaattta ttttgagaaa cataaaataa cggaaatgca
     1561 gcttgttgtc tagtatattg atggttccac tcattagcac aaacaataga ttgtgtatgt
     1621 ggagcatttt ttaatggatt atctacttta tcaagcttgc cat
//
LOCUS       DAOQPK010000342         1653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_74908_length_1653_cov_4.458698, whole genome shotgun sequence.
ACCESSION   DAOQPK010000342 DAOQPK010000000
VERSION     DAOQPK010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74908_length_1653_cov_4.458698"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            244..1536
                     /locus_tag="PLU36_10930"
     CDS             244..1536
                     /locus_tag="PLU36_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012790909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HMZ47308.1"
                     /translation="MKYLLIILGLLFCNIKSFAQTETEQVNNAVLQIKNKVIEWRRHF
                     HQFPELSNREFNTSKKVAEHLKNLGLEVRTGIAKTGVVGILKGGKPGPVVALRADMDA
                     LPVTERNNLPYKSTVTTTFNGQNTGVMHACGHDAHTAMLMGAAEVLSKLKASVPGTVV
                     FIFQPAEEGAPEGEEGGASLMVKEGVMDNPKVDVVFGMHIESDIPAGDLEYKEGAFMA
                     SADLFKIKVKGKSSHGSQPWLGVDPIYVSAQIINAFQGIVSRNSDLTKAPVVITVAAI
                     HSGLRFNIIPEEAEMLGTVRNLDKDIRIKVLEELKAIAEGIAKLHHATAEVTIENKTL
                     VTYNTPQLVKKMLPSLEKAAGKNNVRSRTWVTGAEDFSFYGEKAPAFFFYIGGMSATI
                     KSEQAAPHHTPDFIIDDSKLDVGVKALCQLVFDYSKVK"
BASE COUNT          579 a          214 c          312 g          548 t
ORIGIN      
        1 atttcgggtt ccgatagaat agttttcaaa tgaagtaatt tattaaagat tataatttaa
       61 aaagctaaaa tactaataaa agtttaatgt taccattgct ttatttagtt gtaaaaaagt
      121 gaaatactca aataaaaaag aaagatgttt agcaacagaa accccttcac atgtatatta
      181 tcaatttcat attttgttac tctatatttt ttttttagtt tagtaattta taaatgtaaa
      241 tacatgaaat atttattaat tattttaggc ctcctttttt gcaatataaa aagctttgcc
      301 caaaccgaaa cggaacaagt taacaatgct gttttacaaa taaaaaataa agttattgaa
      361 tggcgtaggc actttcatca atttcctgaa ttgagtaacc gagaatttaa taccagtaaa
      421 aaagtagcag aacatttaaa aaatttaggt ttggaagtta gaactggaat tgccaaaacc
      481 ggtgtggtag gtattttaaa aggtggaaag ccagggcctg ttgtagcact tagagcagat
      541 atggatgctc tgcctgtaac agaaagaaat aatttgcctt ataaatcaac ggtaacaacc
      601 acatttaatg ggcaaaatac tggtgtaatg catgcatgtg gccacgatgc tcatacagca
      661 atgctaatgg gtgctgcaga ggttttaagt aaattaaaag catctgttcc tggtactgtt
      721 gtatttattt ttcaacctgc cgaagaaggt gcaccagaag gtgaagaagg tggtgccagt
      781 ttaatggtaa aggaaggtgt tatggataac ccaaaagttg atgttgtttt tggaatgcat
      841 attgaaagtg atattcctgc aggagattta gaatataaag aaggggcatt tatggctagt
      901 gccgatttgt ttaaaataaa agttaaggga aaaagttctc atggctctca gccttggtta
      961 ggagttgatc ctatttatgt ttctgctcaa attattaatg cttttcaagg tattgttagc
     1021 agaaacagtg atttaaccaa agctcctgtt gtaataactg ttgctgctat tcatagtgga
     1081 ttaagattta atattatacc tgaagaagca gaaatgttgg gtactgtaag aaatttggat
     1141 aaagatatta gaattaaggt tttagaagaa ttaaaggcaa ttgcagaagg tattgcaaaa
     1201 ctgcatcatg caacagccga agttactata gaaaataaaa ctttggtaac ctacaacact
     1261 ccacaactag ttaaaaaaat gttgccatca ttagagaaag cagctggtaa aaataatgtt
     1321 cgttctagaa cttgggtaac tggtgcagaa gatttttctt tttatggaga aaaagcacct
     1381 gccttctttt tttatatagg tggaatgtct gctactataa aatcagagca agctgcacct
     1441 catcatacac cagattttat tattgatgat agtaaattag atgttggtgt taaagcattg
     1501 tgtcaattag tttttgacta ttcaaaagta aaataagtag atattataaa attaataggg
     1561 gctgaattaa aattcagccc ctattaattt atttttcaat tgaattaaat aatttttttt
     1621 ctatttgtgt ttcactttgt gtttggagga acg
//
LOCUS       DAOQPK010000343         1653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_74918_length_1653_cov_3.381727, whole genome shotgun sequence.
ACCESSION   DAOQPK010000343 DAOQPK010000000
VERSION     DAOQPK010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74918_length_1653_cov_3.381727"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..>1653)
                     /gene="aspS"
                     /locus_tag="PLU36_10935"
     CDS             complement(49..>1653)
                     /gene="aspS"
                     /locus_tag="PLU36_10935"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008585009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HMZ47309.1"
                     /translation="ERSNKNKNIATGEIEVTVNNFTILNKSETPPFTIQDETDGGDDL
                     RMKYRYLDLRRNAVKKNIELRYAVNRSARNYLHENGFIDIETPFLIKSTPEGARDFVV
                     PSRMNVGQFYALPQSPQTFKQLLMVSGYDKYYQIVKCFRDEDLRADRQPEFTQIDCEM
                     SFVEQEDILNMFEGLIKSIFKEVKNIDYTSVVERMTWQEAMWQYGNDKPDIRFGMQLC
                     NLKFPAHSFPEKNNISDLIAADLAVFNDAETVVAIAVPDCSEFTRKQTDELTEWVKRP
                     QIGMKGLVFIKCNTDGTFKSSVDKFYSEEKLKAIAQAANAKAGDLILILAGQEERTRK
                     AISELRLEMGKRLGYRKEDEFKLLWVVDFPLFEYAEEENRWVARHHPFTSPKPSDIET
                     MIHNNPAIENAADYLKHPYANIKANAYDMVLNGNEIGGGSIRIYQKELQQKMFAALGM
                     NEEEQQHKFGFLLGAFEFGAPPHGGIAFGFDRLCSILGGSESIRDFIAFPKNNSGRDV
                     MLDAPSSIDTKQLNELQIKLTPKEIN"
BASE COUNT          489 a          321 c          205 g          638 t
ORIGIN      
        1 tactataatt taaaagctca gataaattat ctgagctttt tattttgctt aattaatttc
       61 ttttggagtt agtttaattt gtagttcatt taattgcttt gtatctatac tacttggtgc
      121 atcaagcatt acatctctac cactattatt tttaggaaaa gcaataaagt ctctaatact
      181 ttcgctaccc cctaaaatag aacataatct atcaaaacca aaagcaatac caccatgtgg
      241 cggagcacca aattcaaaag ctcctaataa aaaaccaaat ttatgttgtt gctcttcttc
      301 attcatacct aaggcagcaa acattttttg ctgtaattct ttttgataaa ttctaatact
      361 tcctccacca atttcattac catttaaaac catatcataa gcattggctt taatatttgc
      421 atacggatgt tttaaataat ctgcagcatt ttctatggct gggttgttat gaatcattgt
      481 ttcaatatca cttggcttag ggcttgtaaa aggatgatga cgtgctaccc atctattctc
      541 ttcttcggca tattcaaata aaggaaaatc tacaacccac aatagtttaa actcatcctc
      601 ttttctataa cctaatctct tacccatttc taatcttaat tcgctaatag cttttctggt
      661 tctttcctct tggcctgcaa gtattaaaat taaatctccg gcttttgcat ttgctgcttg
      721 tgcaattgct tttaattttt cttcgctata aaatttatct acactgcttt taaatgtacc
      781 atcagtatta cacttaataa ataccaaacc tttcattcca atttgaggtc gttttaccca
      841 ttcagttaat tcgtctgttt gtttgcgggt aaactcacta caatcaggaa cagcaatggc
      901 tacaacagtt tctgcatcat taaatactgc aagatctgct gcaattaaat cagaaatatt
      961 gtttttttct ggaaagctat gagcaggaaa ttttaagttg catagctgca tgccaaatct
     1021 aatatctggt ttatcatttc catactgcca cattgcttct tgccaagtca ttctttcaac
     1081 tacactggta taatcaatat tttttacttc tttaaaaata ctttttatca aaccctcaaa
     1141 catgttcaaa atatcttctt gttcaacaaa actcatttca caatctattt gtgtaaattc
     1201 tggttgacgg tcagctctta aatcttcatc tctaaaacat tttacaattt gatagtattt
     1261 atcgtaacca cttaccatta ataattgttt aaaagtttgt ggactttggg gcaaagcata
     1321 aaactgccct acattcattc tacttggtac tacaaaatct cttgcacctt ctggcgtaga
     1381 ttttattaag aaaggtgttt caatatctat aaaaccattt tcatgtaaat aatttctggc
     1441 acttctgttt actgcataac gtagttcaat atttttcttt acagcatttc ttctcaagtc
     1501 taaataacga tatttcattc taaggtcatc gccaccatcc gtttcatctt gtatggtaaa
     1561 tggtggtgtt tcacttttgt ttaaaatggt aaagttgttt actgtaactt ctatttctcc
     1621 ggttgcaata tttttatttt tattacttct ttc
//
LOCUS       DAOQPK010000344         1642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_75849_length_1642_cov_3.366730, whole genome shotgun sequence.
ACCESSION   DAOQPK010000344 DAOQPK010000000
VERSION     DAOQPK010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75849_length_1642_cov_3.366730"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1226
                     /locus_tag="PLU36_10940"
     CDS             3..1226
                     /locus_tag="PLU36_10940"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HMZ47310.1"
                     /translation="MKEGLTNTILICNKLNNPQNKFKSVHIAGTNGKGSVSHMLAAIL
                     QTAGFKTGLYTSPHLTDFRERIKVNGKKCSQEFVVQFTEQMKPFIEEFKPSFFEITVG
                     MAFEYFAKEKVDIAIIETGLGGRLDSTNIITPILSIITNIGLDHVQMLGNSLPEIAVE
                     KAGIIKQNVSVIIGETNAATKTVFIKSAQEKNASIQFADELFKVNNYKYSIDNLTIEI
                     ENIKQHTKEVFTLDLTGLYQLKNIITVIAALKKLQDLDWNINNSHIKTALNKTKQLTG
                     LKGRWQVIRKNNPPVFADVAHNSDGVKQVIQHLQLLSFNQLHLIVGMVKDKDVSAVLS
                     LLPSNAKYYFTQAQIPRAMPAEDLQKLAAKFNLEGYSFSNVNLAIEYAIQKASKSDLV
                     LIMGSVFLIGEINSI"
BASE COUNT          636 a          221 c          236 g          549 t
ORIGIN      
        1 ctataaaaga gggcttaacc aatactattt taatctgtaa caaattaaac aatccacaaa
       61 ataaatttaa gtcagtacat attgcaggca ccaatggcaa aggctctgta agccacatgt
      121 tagctgcaat tttgcaaact gctggattca aaacaggctt atatacatcg ccacatttaa
      181 cagattttag ggagagaatt aaagttaatg gtaaaaaatg ctcgcaagaa tttgttgtgc
      241 aatttactga gcaaatgaag ccttttatag aagaatttaa accttctttt tttgaaatta
      301 ccgtaggcat ggcttttgaa tattttgcaa aagaaaaagt ggatattgcc attattgaaa
      361 caggtttagg tggtagatta gatagcacaa atatcatcac accaatttta tccatcatta
      421 ctaatattgg attagaccat gttcaaatgt tgggcaattc attaccagaa attgcagtag
      481 aaaaagcagg aattattaag caaaacgtat cagtaataat tggcgaaaca aatgcagcaa
      541 caaaaacagt ttttattaaa tctgcacagg aaaaaaatgc ttcaatccag tttgcagatg
      601 aactgtttaa agtaaataat tataagtaca gcattgataa cttaaccata gagattgaaa
      661 atattaaaca acacactaaa gaagttttta ctttagatct aactgggttg tatcaactta
      721 aaaatattat cacagtaatt gcagctttaa aaaaattaca agatttagat tggaatatca
      781 ataatagcca tattaaaact gcattaaaca aaacaaaaca gctaacaggt ttaaaaggca
      841 gatggcaagt aatcagaaaa aataatccac cggtttttgc cgatgttgct cataattcag
      901 atggtgtaaa gcaagtaata caacatctac aattactgtc atttaatcaa cttcatttaa
      961 ttgtaggtat ggttaaagat aaagatgtaa gtgcagtttt atctttatta ccttcaaatg
     1021 caaaatatta ctttacacaa gctcaaattc ctagagcaat gcctgcagag gatttacaaa
     1081 agttggctgc taaatttaat ttagaaggct actcttttag caatgtaaat ttagccatag
     1141 aatatgcaat acagaaagca tctaaaagtg atttagtact tataatgggt agtgtttttt
     1201 taattggcga aataaattca atataaccaa taaaaaatat caccccaaaa gggtaaagtt
     1261 ttaaattttt atttctttta caaactagta caaaagcagt ataattttat ttttgtagag
     1321 ctttaataga aaaaagcatt aaagtcatta atatccttat aatcattcag ttttatttag
     1381 ggcttaagct tattttaatt taattattgt ggttatttag aacatattta aattggcatc
     1441 attaacttat tgattgaatg tgctttataa ctatttttta tttattttaa ttaatattag
     1501 gtagaaacta tataaatttt aaggataaaa aataattctt ttaacttcaa aattggctca
     1561 aaatattttt ttcaaaacct gaatgttcca aaacttgcta ttttcttcta acttaaattt
     1621 taatgtttga aatgtgaata aa
//
LOCUS       DAOQPK010000345         1602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_79508_length_1602_cov_3.968326, whole genome shotgun sequence.
ACCESSION   DAOQPK010000345 DAOQPK010000000
VERSION     DAOQPK010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79508_length_1602_cov_3.968326"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="PLU36_10945"
     CDS             complement(<1..472)
                     /locus_tag="PLU36_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HMZ47311.1"
                     /translation="MQHIHKYDAQGKQLCCTQEEKINLIANKQLKKELKEVGCCATDE
                     PQHNEHSAEDGHDHSSDSDSTFKMFLPAIISLVLLLIAIALDNWFPQSWFTGWVRIIW
                     YIVAYAPVGLPVLKDAFTSINKGEIFSEFLLMSIATIGAFAIGEYPEAVAVMLFY"
     gene            complement(477..1076)
                     /locus_tag="PLU36_10950"
     CDS             complement(477..1076)
                     /locus_tag="PLU36_10950"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HMZ47312.1"
                     /translation="MTWLKIYLPIYLLLYLLVAFVIPTYRTYKKTGINPVTFGKSENA
                     HDYIGFVMKILIALLFFSVLIFSIFQNFYLYLCPIHFLQNSIVQLIGLVLIHFSLIWI
                     SVAQFQMSNSWRIGIDENNRTELVTTGIFSISRNPIFLGMILSVLGLFLITPNAIMFC
                     LTFTTYIIIQIQIRLEEEFLERQHTKNYINYKQKTRRLI"
     gene            complement(1088..>1602)
                     /locus_tag="PLU36_10955"
     CDS             complement(1088..>1602)
                     /locus_tag="PLU36_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018628292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47313.1"
                     /translation="KVGQTIHFTLTNNPTTEYDAVVYSIGTAFENDSKTIPVHCNVKG
                     NKTGLIDGMNITGIVSLNNVTTPAVPTDAIVEADGKYYIFIVTNREPEEHHEEGEEAH
                     DHKEAEKSTETEPKKHTNFEKIEVVKGVSNMGYTSITFINEIPKDAKVVTKGAFFINA
                     KLSNTGEHEH"
BASE COUNT          508 a          254 c          239 g          601 t
ORIGIN      
        1 catagaataa cattactgct acagcttctg ggtattcgcc tatggcaaat gctcctattg
       61 tagcaatgct cattaataga aattcagaga atatttctcc cttattaata cttgtaaaag
      121 catcttttaa tacaggtaag ccaactggtg catacgctac gatataccat ataatacgaa
      181 cccaacctgt gaaccaagat tgaggaaacc aattatctaa tgcaatggct ataagtaata
      241 aaaccaaact tattatggct ggtaaaaaca ttttgaaagt gctatcgcta tcacttgaat
      301 gatcgtgtcc atcctctgca ctatgttcat tgtgttgagg ttcatctgtt gcacagcaac
      361 ctacttcttt aagttctttt tttaattgct tattagcaat aagatttatt ttttcttctt
      421 gtgtgcaaca aagttgtttt ccttgtgcat cgtatttgtg aatgtgttgc atattattaa
      481 attaatcttc ttgttttttg tttgtaattg atataattct ttgtgtgctg tctttctaaa
      541 aattcttctt ctaatcttat ttgaatttga ataattatat aggtagtaaa tgttaaacaa
      601 aacattattg catttggtgt tattaaaaac aagccaagca cacttaaaat catccctaaa
      661 aaaataggat ttctacttat tgaaaaaatg cctgttgtaa ctaattcagt tctattgttt
      721 tcatctattc caatacgcca actattgctc atttgaaatt gtgctacact tatccaaatt
      781 agagaaaagt gaattaacac aagaccaatc aattgaacaa tagaattttg taaaaaatga
      841 attgggcata agtacaaata gaaattttga aaaattgaaa atattaaaac agaaaaaaat
      901 agtaaagcaa taagtatttt cattacaaat ccaatataat cgtgtgcatt ttcagacttg
      961 ccaaatgtaa caggatttat acctgttttc ttataggtac gataagtcgg tattacaaat
     1021 gcaactaata aataaagtag taagtaaata ggtaaataaa tttttaacca agtcatattg
     1081 ttatagttta atgttcgtgt tcgcctgtgt tagataattt ggcattgata aagaatgcac
     1141 cttttgttac tactttagca tctttgggta tttcatttat aaatgtaata gaagtataac
     1201 ccatattgga aacacctttt accacctcta ttttttcaaa gtttgtatgc tttttaggtt
     1261 ctgtttctgt acttttttct gcttctttat ggtcgtgtgc ttcttctcct tcttcgtggt
     1321 gttcttcagg ttctctattg gtaacaataa aaatataata cttgccatcg gcttctacaa
     1381 tagcatctgt tggtactgct ggtgtggtta cattatttaa gctaacaatg cctgtaatgt
     1441 tcataccatc tataagccct gttttattgc cttttacatt gcaatgtaca ggtatggttt
     1501 tactgtcgtt ttcaaaggca gtacctatac tatatactac agcatcatat tcagtagttg
     1561 ggttattagt taatgtaaaa tgaatggttt gcccaacttt ta
//
LOCUS       DAOQPK010000346         1592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_80492_length_1592_cov_2.372804, whole genome shotgun sequence.
ACCESSION   DAOQPK010000346 DAOQPK010000000
VERSION     DAOQPK010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80492_length_1592_cov_2.372804"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..375
                     /locus_tag="PLU36_10960"
     CDS             85..375
                     /locus_tag="PLU36_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47314.1"
                     /translation="MTYLINLYYSVYTLATTFENSEEILDVLITVHEEAYSGYCCSLT
                     KDNKVDWSCCNFWESIKVTVKAAFTCNLVQFPSREDVYNFYLCYQSIICQTC"
     gene            complement(834..1556)
                     /locus_tag="PLU36_10965"
     CDS             complement(834..1556)
                     /locus_tag="PLU36_10965"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HMZ47315.1"
                     /translation="MLKIKETIEKVAITDARVLITGENGSGKELVAKWLHEKSNRNTG
                     PLIEVNCAAIPSELIESELFGHEKGSFTSAIKQRIGKFEQANGGTLFLDEIGDMSLSA
                     QAKVLRALQEGKITRVGGDKEINVDVRVVAATNKNLIAEVEEKNFRLDLYHRLSVILI
                     HVPSLNERREDIPLLVDIFLEDVANEYRQTKKAIDKKALEALQQHNWTGNIRELRNVI
                     ERLVMLSSKNICLEDVKNYVVY"
BASE COUNT          512 a          266 c          228 g          586 t
ORIGIN      
        1 ctatttgctg ttttgaacga tatctttatt gccagcgtgt gaggcggttt tttaatcaat
       61 tattgtatgg ataaaattac cacattgacc tacctaatta acttatatta ttcagtatat
      121 actttagcga caacgtttga aaattcagaa gaaattttag atgtattaat tactgtgcat
      181 gaagaagctt attcagggta ctgttgctcg ttaacaaaag ataataaagt agattggagt
      241 tgttgtaatt tttgggaaag tataaaagtt actgttaaag cagcttttac ttgcaattta
      301 gttcagtttc catctcgtga agatgtttat aatttttatc tttgttatca atcaataatt
      361 tgtcaaacat gttaaactaa taaatcatga agaagagaaa attcataaag tattccctta
      421 tcagcgtggt ttcaatattg cttattttct ttgtttatca atataaaaaa gcaagagata
      481 caggcaattt aacaccagag gagtgggtaa aaaagtatag ataaaaagtt aggttgtctt
      541 aaattacaag tagcccacct ataaatagct tattttatta aatgacgtaa actcggcata
      601 cgcttttagc tatgccgttt tttatgtagg tttatactgc caatgataga taaaatactt
      661 tacataattg aaggtgcaac ggagcaggga aaactacaac ttcatttact attttgcctg
      721 aaattataag ccaacaaaaa ttttgttacc agcgatacca ttcgcaacga acttttacga
      781 ttgggtgtac agttaaaaga tgaaaaagat ggaaatataa gttggagtat agactaatac
      841 accacatagt tttttacatc ctctaaacaa atattttttg aagaaagcat aaccaacctt
      901 tcaataacat ttctaagttc tctaatattg cctgtccagt tatgttgttg taaagcttct
      961 aatgcttttt tatctatagc ctttttggtt tgcctatatt cattagcaac atcttctaaa
     1021 aatatatcca ctaaaagagg tatatcttct cttctttcat ttaaagaagg cacatgaatt
     1081 agtattacac taagcctatg gtacagatct aatctaaaat ttttctcctc tacttctgca
     1141 attaaatttt tattagttgc tgcaactact cttacatcaa catttatttc tttatcacca
     1201 cccactcttg taatttttcc ttcttgtaaa gcccttaata ctttagcttg tgcactcagg
     1261 ctcatatcgc caatttcatc taaaaaaagg gtgcctccat tagcttgctc aaattttcct
     1321 attcgttgtt ttatagcact ggtaaacgaa cctttttcat gtccaaacaa ttcgctttct
     1381 atcaattcag aagggatggc tgcacaattt acttcaatta gtggccctgt atttctgttg
     1441 cttttttcat gtaaccattt tgcaacaagt tctttaccac ttccattttc gcctgttatt
     1501 aaaacccttg catcagtaat agcaactttt tcaattgttt ctttaatttt taaaatagct
     1561 tgaacaaaat ggtacagtta aaattgtaaa tt
//
LOCUS       DAOQPK010000347         1584 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_81276_length_1584_cov_4.243950, whole genome shotgun sequence.
ACCESSION   DAOQPK010000347 DAOQPK010000000
VERSION     DAOQPK010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81276_length_1584_cov_4.243950"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PLU36_10970"
     CDS             complement(<1..560)
                     /locus_tag="PLU36_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HMZ47316.1"
                     /translation="MDIKNNILETIGNTPLIKLNKVTKDFPCTVLAKVDYFNPGNSIK
                     DRMALKMVEVAEKGGKLKPGGTIIECTSGNTGMGLALAACVKGYKCIFVTTDKQSKEK
                     ADILKAVGAEVIVCPTNVMPEDPKSYYSVAKRLATEIPNSYHFNQYDNLANRLAHYES
                     TGPEIWKQTDGKITHLVCTAGTGGTIT"
     gene            complement(637..1443)
                     /locus_tag="PLU36_10975"
     CDS             complement(637..1443)
                     /locus_tag="PLU36_10975"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ47317.1"
                     /translation="MTKNEQYFSINKDAWNKKTPVHVESSFYNQEAFLNGKNSLNDIE
                     LDLLGNIQDKNILHLQCHFGQDTISLSRLGAIATGVDIAENAINIAKQTANQLNLQTQ
                     FICCNIYDLSNYLNQKFDIVFTSYGTIGWLPDLDKWAKIINHFLQPNGQFIMVDFHPV
                     VWMFDNDFQQVTYNYFNTGAILENEVGTYANKKTDIQFQTASWNHSIGEILNSLISNQ
                     FSINSFDEYDYSPYNCFNNTIEVETNKFRIKHMGNKLPMLYAIKATKDKT"
BASE COUNT          503 a          249 c          212 g          620 t
ORIGIN      
        1 gtaattgtac ctcctgttcc tgcagtgcaa acaagatgtg ttatttttcc atcagtttgt
       61 ttccaaattt ctggtcctgt actttcgtaa tgtgctaaac ggtttgctaa attatcgtac
      121 tgattaaagt ggtatgaatt gggtatttca gtagctaagc gtttggccac actataataa
      181 cttttaggat cttctggcat tacattggtt gggcaaacaa taacttctgc acccactgct
      241 tttaaaatat ctgctttttc tttactctgc ttatctgttg taacaaaaat acatttgtat
      301 ccttttacac aagctgctaa ggcaagtccc atacctgtgt tgccactagt acattcaata
      361 atggttccac ctggtttaag tttacctcct ttttcggcta cttctaccat ttttaaagcc
      421 attctatctt taatgctatt gcctgggtta aaataatcta ctttagctaa aacagtacag
      481 ggaaaatctt ttgtaacttt atttaactta attaagggtg tattaccaat agtttctagt
      541 atgttatttt taatatccat agcaaaaaat ttgatgcaaa tgtaagagtt tagaattgga
      601 tacccttaaa cactattatt gaaaattagt ttatgtttac gttttatctt ttgtggcttt
      661 tatagcataa agcatgggta gtttattgcc catatgtttt attctaaatt tattagtttc
      721 cacttcaatt gtattattaa aacaattgta aggtgaataa tcatactcat caaaactatt
      781 aatactaaat tggttactaa ttaaactatt aagtatttcg ccaatgctat gattccagga
      841 agctgtttga aattgaatat cagttttttt gtttgcataa gtacccactt cattttcaag
      901 tattgcacct gtgttgaaat agttataagt tacttgttga aaatcgttat caaacatcca
      961 aacaacaggg tgaaaatcta ccataataaa ttgcccattg ggttgtaaaa aatggttaat
     1021 aatttttgcc catttatcta aatctggcaa ccaaccaatg gttccataac tggtaaaaac
     1081 tatatcaaat ttttggttta aataatttga taaatcataa atattacagc atataaattg
     1141 tgtttgcaag tttagttggt tagcagtttg ttttgctatg tttattgcat tttctgctat
     1201 atctacacct gttgcaattg cacctaacct acttaatgaa attgtatctt gcccaaaatg
     1261 acattgcaaa tgcaaaatat ttttatcttg tatatttcct aataaatcta attcaatatc
     1321 gtttaaggaa ttttttccat ttaaaaatgc ttcttggtta taaaaagaag attctacatg
     1381 caccggtgtt ttcttattcc atgcatcttt attaatacta aaatattgtt catttttagt
     1441 cataaaaatt taattatatc tgtttgccat aaattaagaa ccatattaaa aataaagcaa
     1501 cattatttat aaattctatt ttattttcgc cactttagaa aaataaattt atgaaacttc
     1561 aattaattaa tccaattgcg ttta
//
LOCUS       DAOQPK010000348         1579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_81748_length_1579_cov_5.484908, whole genome shotgun sequence.
ACCESSION   DAOQPK010000348 DAOQPK010000000
VERSION     DAOQPK010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81748_length_1579_cov_5.484908"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="PLU36_10980"
     CDS             complement(<1..1284)
                     /locus_tag="PLU36_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47318.1"
                     /translation="MKYLFPILYLLILINSVNAQIAPPQAFNYSGVARNASNSPIANQ
                     TIGIQLSILKGSAVTGTVVYKENHFTNTDALGLFNIAVGTGAVQSGTFSTIDWSNDTY
                     YLRVGLDATGGTNFVTMGSTQLLSVPYALYAKSAGTATSATDHDTSATNELQSLSVRK
                     DTIFLTNGGFVKVPASSFDHDTSASNELQTLSIRNDTIFLTNGGFVKLPTGSASGTKP
                     TVTTGSATSITSTSATLQGNVTNDGGEIILTRGLCLSTSTNPTTANLLMTTSGYLGSY
                     NFNASNLTTGTVYYVRAFATNTNGTSYGNQVSFTTLNTCFQQEDKLYGYYGGAINYLS
                     GGGIDSVTIDNNDSVDISNTIFSDCILNYLNDRFSAASYTNISLPSGGTSFTTPNQTV
                     SNIYLNRSNNESYIQNPTVNITCQNITATQFSIIIN"
BASE COUNT          459 a          267 c          288 g          565 t
ORIGIN      
        1 attaatgata atactgaatt gagtggccgt tatattctga catgtaatat ttactgttgg
       61 attctgaatg taagattcat tattacttct atttaagtat atattactca cagtttgatt
      121 aggtgttgta aatgatgtac cgcctgatgg taacgaaata ttggtgtaac ttgcagcgct
      181 aaacctatca ttaaggtaat ttaagatgca gtctgaaaaa atggtgttac ttatgtctac
      241 tgaatcatta ttatcaatgg ttactgaatc aattccacct ccggataaat aatttatagc
      301 tccaccataa taaccataaa gtttgtcttc ttgttgaaaa caagtgttca gtgttgtaaa
      361 ggaaacttga tttccatacg aagttccgtt tgtgttagta gcaaatgctc ttacataata
      421 tacagttcct gtcgttaagt tacttgcgtt aaaattatag ctgcctaaat agccgctggt
      481 tgtcattaat aaattagctg ttgtaggatt agtcgaagtg cttaagcaca gtcctctggt
      541 caatatgatt tcacctccat cattagtcac attaccttgt agtgtggctg atgttgatgt
      601 tattgaagtg gcgctgccgg tcgttaccgt tggtttagta ccactggcac ttcctgtcgg
      661 caattttaca aaacctccgt tagttaggaa aatggtatca tttctaatac ttaaagtttg
      721 taattcattt gatgcactcg tgtcgtgatc aaaactactt gcaggtactt tgacaaaccc
      781 tccattggtc aagaaaatag tatcttttct tacacttaat gactgtagtt cattagttgc
      841 gctggtatca tggtccgttg cgctagttgc agttcctgcg cttttagcat ataacgcata
      901 cggtacactt aagagttgtg ttgaacccat tgttacaaag tttgtacctc cggttgcatc
      961 caatcctact ctgagataat aagtatcatt gctccagtct attgtagaaa aagttccgct
     1021 ttgaacggca cctgtgccaa cagcaatatt aaacaaacct aatgcgtctg tattggtaaa
     1081 gtggttttct ttgtaaacaa ctgttcctgt tactgcacta ccctttaata tacttagctg
     1141 tatacctatt gtttgattag caattgggct gttactggca tttcgcgcca cgccactgta
     1201 gttgaatgct tgaggcggag ctatttgtgc atttacacta ttgattaaga ttaataaata
     1261 caggatggga aaaagatact tcatagtttt aagaatttta aaggtgtatt aattttatga
     1321 ctttcggtaa aaatacgaat atttataatt ataaacctgt tgttgcaaat ttttttaaat
     1381 aaagtttttt acaaacaaat tgctatccgt tttgaagcct tagacatctt tgcagtgtaa
     1441 tgcagtaatc tgtgtatatc tttctacttt ataacatgca aagaatcagg ctaataatta
     1501 aatctccgcc ttagttggtg ttcttcacca taagtgttcg gaagattacg ttgtattata
     1561 agaatagaga aattgattt
//
LOCUS       DAOQPK010000349         1576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_82076_length_1576_cov_3.005260, whole genome shotgun sequence.
ACCESSION   DAOQPK010000349 DAOQPK010000000
VERSION     DAOQPK010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82076_length_1576_cov_3.005260"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PLU36_10985"
     CDS             <1..211
                     /locus_tag="PLU36_10985"
                     /inference="COORDINATES: protein motif:HMM:NF037182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ47319.1"
                     /translation="HLFNDYISGPAQPVVNRIRNLYLWEILIKLPKNADLMHQCKTII
                     KQQAAIIHNTKNLSGVIIIPDVDPV"
     gene            303..>1576
                     /locus_tag="PLU36_10990"
     CDS             303..>1576
                     /locus_tag="PLU36_10990"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HMZ47320.1"
                     /translation="MKNKFYQIALIVTVIIISSFSFKENRTSPSIQFPYKKQGLTKEQ
                     AAAHLLSRFTFGVSQNDINQVVNEGLENWFLHQLDGNLPDDVLEEKLQGNDLLTFSNE
                     DILKKYFKNNQILRAALKEGVITKEEATNKKEADIKQKLAAYMKQKGWLPVRELMRLQ
                     INNKVLRAAYTNNQLVEVMTDFWYNHFNIAATKNQCVQFIPVYERDAIKRNALGNFEN
                     LLIATAKSPAMLLYLDNAMSSSNNNELATKLENRRNNFSNKENKKLNKKRSSGLNENY
                     AREVMELHTLGVDGGYTQNDVTEVARVLTGWTIYPISDNGPLSNLKKMIEKNKDKFTE
                     NGFVRDGDFLFAANRHDDGEKIILGKKFNSDGGYQEGIMLLKMLANHQSTAKFISKKL
                     AVRFVCDNPSSALIDKMAKTFLLTNGNIKQVIL"
BASE COUNT          623 a          203 c          257 g          493 t
ORIGIN      
        1 acatttattt aatgattata ttagtgggcc tgcacaacca gttgtaaata gaattagaaa
       61 tttataccta tgggaaattc taattaaact tcccaaaaat gccgatttaa tgcatcaatg
      121 taaaactatt attaaacaac aagcagctat tattcataat actaaaaact taagtggtgt
      181 tattattatt ccagatgtgg atcctgtgta aatatttctg attttcttct ttaaaccttg
      241 gtatcccaaa taagtctatt gttatgtgtt aaattagata cgcaactaat aaagtacaga
      301 acatgaaaaa taaattctat caaattgctt taattgttac tgtaataatt atatcctctt
      361 tttcatttaa agaaaataga acttctcctt ccattcaatt tccttataaa aaacaaggtt
      421 taacaaaaga gcaagcagca gcacatttat taagcagatt tacttttgga gtttctcaaa
      481 atgacattaa tcaagttgta aatgaaggct tggaaaattg gtttttacat caattagatg
      541 gcaatttgcc cgatgatgtt ttagaagaga aattacaggg taacgattta ttaaccttta
      601 gtaacgaaga tattttaaaa aaatatttta aaaataacca aatattaagg gctgctttaa
      661 aggagggtgt aattacaaaa gaagaagcaa caaataaaaa ggaagcagat ataaaacaaa
      721 aacttgcagc ttatatgaag caaaaaggtt ggttgcctgt aagagaatta atgaggctac
      781 aaataaataa taaagtttta agagccgctt atactaataa ccaacttgta gaggttatga
      841 ctgatttttg gtataaccat tttaatatag cagctaccaa aaaccaatgt gtacaattta
      901 ttcctgtgta cgaaagagat gcaattaaaa gaaatgcgtt aggtaatttt gaaaatttat
      961 taattgcaac agcaaaatcg cctgctatgc ttttgtattt agataatgca atgagttcct
     1021 ccaataacaa tgaattagca acaaaattag agaacagaag aaataatttt tccaacaaag
     1081 aaaataagaa acttaacaag aagagaagta gtggattaaa cgagaattat gcaagagaag
     1141 ttatggaatt gcacacttta ggagtagatg gtggttatac acaaaatgat gtaacagaag
     1201 tggcaagggt tttaacaggc tggacaattt accccatttc tgataatggg cctttaagca
     1261 atttgaaaaa aatgattgaa aaaaataaag acaaatttac tgaaaatggt tttgtaagag
     1321 atggagattt cttgtttgct gcaaatagac atgatgatgg tgaaaaaatt attttaggaa
     1381 aaaaatttaa ttcagacggt ggttatcaag aaggaattat gcttctaaaa atgttggcta
     1441 atcatcaatc cacagccaaa tttatcagta aaaaattagc tgttaggttt gtatgcgata
     1501 atccttcctc agcccttatt gataaaatgg ctaaaacatt tttattaacc aatggtaata
     1561 ttaaacaagt aatact
//
LOCUS       DAOQPK010000350         1550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_84765_length_1550_cov_3.995318, whole genome shotgun sequence.
ACCESSION   DAOQPK010000350 DAOQPK010000000
VERSION     DAOQPK010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84765_length_1550_cov_3.995318"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="PLU36_10995"
     CDS             complement(<1..991)
                     /locus_tag="PLU36_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HMZ47321.1"
                     /translation="MQLIKRTLWLLLILTVSFSYAQNTPLPVDPSVKIGQLANGLKYY
                     IKSNKKPEKKVELRLVINAGSILEDDDQQGLAHMVEHMAFNGTTNFKKNDIISYLQSI
                     GVGFGNDLNAYTSFDETVYMLPIPTDKPGNLEKGFQIIEDWAHNVTFLDDDIDGERNI
                     ILEESRLGKGAEDRMFKKVYPILFEGSKYGNRLPIGIESIIKTFKHDAIRRFYRDWYR
                     PNLMAVVVVGDIDIATAEAYVKKHFTGLKNPENERPRTNFDVPAYKANQGIVVTDKEA
                     TMYVSMLNYSAEKHHEATNIDEYKASIIQSLFTTILNQRLREVTQQTNPPFLGA"
     gene            complement(1224..>1550)
                     /locus_tag="PLU36_11000"
     CDS             complement(1224..>1550)
                     /locus_tag="PLU36_11000"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HMZ47322.1"
                     /translation="SSLTMNLVYFNPNEFGVDLRKVNCDVFIDNNFFGKYSLDTLMHI
                     KRRSEFALPSRIDIEMKGIYKNLYNVIFNKEIQLNVKGTAKVGKAGIFVNMPFNYNGK
                     HTINFF"
BASE COUNT          499 a          245 c          214 g          592 t
ORIGIN      
        1 atgcacctaa aaatggtggg ttggtttgtt gggtaacttc tcttaatctt tgattaagaa
       61 tagtggtgaa taaagattga ataatacttg ctttgtactc atctatattg gttgcttcat
      121 gatgtttttc agcactatag ttaagcatag aaacatacat ggtagcttct ttatctgtaa
      181 caacaattcc ttgattggct ttataagctg gcacatcaaa gtttgttctt ggcctttcgt
      241 tttccggatt ttttaagcct gtaaaatgtt tctttacata ggcttcagct gttgcaatat
      301 caatatcacc tacaacaaca acagccatta aatttggacg ataccaatct ctataaaaac
      361 gacgaatagc atcgtgttta aatgttttaa ttatactttc aattccaatt ggtaaacgat
      421 taccatattt actgccttca aaaagtatag gatacacttt tttaaacatt ctgtcttctg
      481 cacctttgcc taaacggctt tcttctaaaa taatatttct ttccccatca atatcatcat
      541 ctaaaaaagt aacattgtgt gcccaatctt ctataatttg aaatcctttt tcaaggtttc
      601 ctggtttatc tgttggtatg ggtaacatat aaacagtttc atcaaaactg gtatatgcat
      661 tcaaatcgtt accaaagcct acaccaatac tttgtaaata ggatataata tcattctttt
      721 taaagttggt ggtaccatta aacgccatat gttctaccat atgtgctaaa ccttgttggt
      781 catcatcttc taaaattgaa cctgcattaa ttactaaacg caattcaact tttttctctg
      841 gttttttatt gcttttgatg tagtacttta agccatttgc taattgacca attttaacac
      901 ttgggtcaac cggcaatgga gtgttttgtg catagctaaa acttacggtt agtataagta
      961 aaagccacaa tgttctttta ataagttgca tattttaaaa tttattgttg cgaagtaatg
     1021 aaatgaaaaa gaagtaaaca aaaaagagtt tagattattt tgtggttatt tgcaaaggag
     1081 ctattatttc ttatttttta acttttctat ctatacaaat tatagaaaaa gaaagtggtt
     1141 tttattttaa ttgtacaatt aataaataag ttgtaaaaat tgccaaaaca ctatatttac
     1201 ttttctattt taattcgttt aacttaaaaa aagttgatag tatgcttgcc gttgtaatta
     1261 aaaggcatat ttacaaaaat accagctttg ccaacttttg ctgttccttt tacatttagt
     1321 tgaatttctt tattaaaaat tacgttgtat aagtttttat agatgccttt catttctata
     1381 tcaatacggc ttggcaaagc aaattcactt cttcttttaa tgtgcattag ggtatctaaa
     1441 gaatattttc caaaaaaatt gttgtctata aatacatcac aatttacttt tcttaaatca
     1501 acaccaaatt catttgggtt aaagtacacc aaattcatgg ttaaactaga
//
LOCUS       DAOQPK010000351         1543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_85482_length_1543_cov_4.229167, whole genome shotgun sequence.
ACCESSION   DAOQPK010000351 DAOQPK010000000
VERSION     DAOQPK010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85482_length_1543_cov_4.229167"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..643
                     /gene="rsmD"
                     /locus_tag="PLU36_11005"
     CDS             104..643
                     /gene="rsmD"
                     /locus_tag="PLU36_11005"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HMZ47323.1"
                     /translation="MRIISGKFGGRKINPPSKMPHTRPTTDIAKEGLFNILQNRISFD
                     QIKTLDLFGGTGSISYELASRGASHLTIVEKDAVMYNFIKNNIAQLKIENCQVFKLDI
                     FNFLATCTEKYDFIFAGPPYALGSIDEIPKIIMQKQLLAPNGYFVLEHTPRNNYETFT
                     GFSFVRNYGTTLFSFFIAE"
     gene            717..1481
                     /gene="lptB"
                     /locus_tag="PLU36_11010"
     CDS             717..1481
                     /gene="lptB"
                     /locus_tag="PLU36_11010"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018615084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HMZ47324.1"
                     /translation="MSLTIHTNNLIKTYKSRTVVNNVSIKLQQGEIVGLLGPNGAGKT
                     TTFYMVVGLIKPDDGNVLLNDSNITKLPMYKRAQMGIGYLPQEASVFRKLSVEDNIMS
                     VLEMTKKNKTQRKEKLEELLDEFHLHHVRKNNGDSLSGGERRRTEIARALAVDPKFIL
                     LDEPFAGVDPIAVEDIQSVVAKLKYKNIGILITDHNVNETLSICDRAYLLIDGKIFKH
                     GTAEELANDDQVKRLYLGTNFELKRKDWIIEMGNSQ"
BASE COUNT          585 a          211 c          242 g          505 t
ORIGIN      
        1 tttcaaatgt attaattgca tctcccaatg ccgatgaaat ggcatttgaa actaatgcac
       61 aatttccagc atattatttt accccatttt ttaatttaca agtatgagaa taatttcagg
      121 aaaatttgga ggaagaaaaa taaatccacc ttcaaaaatg cctcatactc gtcctacaac
      181 agatattgcc aaagaaggat tatttaatat tctgcaaaat agaatttctt tcgatcaaat
      241 aaaaacatta gatctttttg gtggtacagg aagtattagt tacgaattag catcacgtgg
      301 tgctagccat ttaaccattg tagaaaaaga tgctgttatg tacaatttta taaaaaataa
      361 tattgcacaa ttaaaaattg aaaattgcca ggtttttaag ttagatattt ttaatttttt
      421 agcaacctgt acagaaaagt acgattttat ttttgctggt ccaccttatg cattaggctc
      481 tattgatgaa attcctaaaa taattatgca aaaacagcta cttgcaccca atggttattt
      541 tgtgttagaa cataccccaa gaaacaatta tgaaacattt acaggctttt cttttgtaag
      601 aaattatggc actacattgt tcagcttttt tattgctgaa taaaatgatc ttaaataaaa
      661 tattttaaaa taaaatatca tgtacattaa ctttattttt gtttaaatct tttacattga
      721 gcctaacaat tcataccaac aatttaataa aaacatataa gagcagaact gttgtaaata
      781 acgttagcat aaaattacaa caaggcgaaa tagttggctt acttggtcca aatggtgctg
      841 gtaaaacaac tactttttat atggtggttg gcttaataaa accagatgat gggaatgtgt
      901 tattaaatga tagtaatatt acaaaattac caatgtacaa acgtgcccaa atgggcatag
      961 gttatttacc acaagaggca agtgttttta gaaaactatc tgttgaagac aacattatga
     1021 gtgtattaga aatgactaaa aaaaataaaa cgcaacgaaa agaaaaatta gaagaattgc
     1081 tagatgagtt tcacttacat catgtaagaa aaaataatgg agatagttta agtggtgggg
     1141 aaagaagaag aacagaaatt gcaagagcat tagcagtgga tcctaaattt attttattag
     1201 atgaaccttt tgcaggtgtt gacccaattg ctgtagaaga tatacaaagt gtagttgcaa
     1261 agctgaagta taaaaatata ggaatattaa ttaccgacca taacgttaac gaaacccttt
     1321 ctatttgcga tagagcctat ttattaattg atggtaaaat atttaaacat ggcactgccg
     1381 aagaattggc aaatgatgat caggtaaaac gtttatacct tggaactaat tttgaattaa
     1441 aacgaaaaga ttggataatt gaaatgggaa attcccaata ataaattatt tactttcata
     1501 ttaccattga ttttcagttt cattctcctt aatattattt tac
//
LOCUS       DAOQPK010000352         1520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Chitinophagaceae bacterium isolate AMR_MDS_0446
            NODE_88057_length_1520_cov_3.343345, whole genome shotgun sequence.
ACCESSION   DAOQPK010000352 DAOQPK010000000
VERSION     DAOQPK010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810078
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chitinophagaceae bacterium (wastewater metagenome)
  ORGANISM  Chitinophagaceae bacterium
            Bacteria; Bacteroidota; Chitinophagia; Chitinophagales;
            Chitinophagaceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:27:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,169
            Genes (coding)                    :: 2,165
            CDSs (with protein)               :: 2,165
            Genes (RNA)                       :: 34
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 1 of 4
            Pseudo Genes (internal stop)      :: 2 of 4
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Chitinophagaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88057_length_1520_cov_3.343345"
                     /isolate="AMR_MDS_0446"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1869212"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..1489
                     /locus_tag="PLU36_11015"
     CDS             53..1489
                     /locus_tag="PLU36_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018611374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HMZ47325.1"
                     /translation="MFKKLDTLILRSFVAPFFATFFISLFVLVMQFFWLYIDDLVGKG
                     LEVTTIFKLIAYVTATLVPLALPLAMLLSSIMTFGNLGETFELVAIKSSGISLLRFMR
                     PLLIVAIAISGLAFVFANNVIPVAQLKLAALKYDIIIAKPAFDIKEGVFYNQIKDFAI
                     KIGKKEKDDSTIKNIVIFEKRYGIQDNIIVADSGIMKVSNNKKFLEFVLKDGWRYEEK
                     AQANSATNTDYIRLGFKEYKKIFDLRSLQMNQSADSSFRYDPKMLSIRQLNYSIDSFK
                     TIQQKFIKRNNTEVAETLPFVQYVNATFKQAKPLKHVNNITAIIPDSIRTIIFDNANS
                     KLNIARNTTAIAASDYEFKNESKINHQIEWHRKFTLSFACIVLFLIGAPLGSIIRKGG
                     LGLPLVFSIIFFIIFHMINTFGEKFVEESVASPFVGMWLSSMILLPIGFFLTFKAMRD
                     SQLFNQEFYYRTFKGVREFIKKFKKIKV"
BASE COUNT          555 a          191 c          237 g          537 t
ORIGIN      
        1 gctacataaa taagtacatt tgctacattg aataaaaatt agaaaagcaa tagtgtttaa
       61 aaaattagat acattaatac tccgatcttt tgtagcccct ttctttgcta ctttttttat
      121 ttcgttgttt gtattagtaa tgcaattctt ttggctttat attgatgatt tagtaggcaa
      181 aggattagaa gttacaacca tttttaaatt aattgcttat gttactgcaa ctttagttcc
      241 attggcttta ccattggcta tgttactttc atccataatg acatttggca acttaggcga
      301 aacctttgaa ttagttgcaa taaaatcatc gggtatttct ttattaagat ttatgaggcc
      361 cttactaatt gtggctatag caattagtgg cttggcattt gtttttgcaa ataatgtaat
      421 acctgttgct caattaaaat tagcagcttt aaaatatgat atcataattg caaagcctgc
      481 ttttgatata aaagaaggtg tattttataa ccaaataaaa gattttgcaa taaaaattgg
      541 taagaaagaa aaagatgata gtactataaa aaatattgta atttttgaaa agaggtatgg
      601 catacaagat aatattattg ttgcagatag tggtataatg aaggtttcta acaacaaaaa
      661 atttttagaa tttgttttaa aagatggttg gcgatatgaa gaaaaagcac aagcaaatag
      721 tgctacaaat accgattata tcagacttgg atttaaagaa tataaaaaga tattcgattt
      781 aagatcgttg caaatgaacc aatctgccga ctctagtttt aggtatgatc ctaaaatgct
      841 aagcattagg caacttaatt attcaataga ttcttttaaa acaattcaac aaaaatttat
      901 taaaagaaat aatacagaag tagcagaaac cctaccattt gtgcaatatg taaatgctac
      961 tttcaaacag gctaaaccat taaagcatgt aaacaatatt actgctataa taccagatag
     1021 tataagaact attatttttg ataatgctaa tagtaaatta aatatagctc gcaacacaac
     1081 tgctattgca gctagtgatt atgaatttaa aaatgaatcc aaaattaatc atcaaataga
     1141 atggcataga aagtttactt taagttttgc ttgtattgtt ttatttttaa ttggtgcacc
     1201 tttgggctcc ataataagaa aaggtggctt aggcttacca ttagtatttt ctattatttt
     1261 ctttatcatt tttcacatga taaacacttt tggcgaaaaa tttgtggaag agtctgtagc
     1321 atctcctttt gttggtatgt ggttaagttc aatgatactg ttgcccattg gcttttttct
     1381 tacctttaaa gcaatgagag actctcaatt gtttaaccag gagttttatt atagaacatt
     1441 taagggagta cgtgaattta ttaaaaaatt taaaaaaata aaagtttagt gtatgaaaaa
     1501 taacaaaata aatagaagaa
//
